LOCUS       DACECR010000001        72767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.001, whole genome shotgun sequence.
ACCESSION   DACECR010000001 DACECR010000000
VERSION     DACECR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.001"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(103..1728)
                     /gene="groL"
                     /locus_tag="IWO17_000001"
     CDS             complement(103..1728)
                     /gene="groL"
                     /locus_tag="IWO17_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ0288911.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(1779..2063)
                     /locus_tag="IWO17_000002"
     CDS             complement(1779..2063)
                     /locus_tag="IWO17_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ0288912.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            2288..2950
                     /locus_tag="IWO17_000003"
     CDS             2288..2950
                     /locus_tag="IWO17_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0288913.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(3026..4318)
                     /locus_tag="IWO17_000004"
     CDS             complement(3026..4318)
                     /locus_tag="IWO17_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0288914.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            4488..5117
                     /locus_tag="IWO17_000005"
     CDS             4488..5117
                     /locus_tag="IWO17_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAQ0288915.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(5220..6029)
                     /locus_tag="IWO17_000006"
     CDS             complement(5220..6029)
                     /locus_tag="IWO17_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0288916.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(6084..6953)
                     /locus_tag="IWO17_000007"
     CDS             complement(6084..6953)
                     /locus_tag="IWO17_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0288917.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(6954..8270)
                     /locus_tag="IWO17_000008"
     CDS             complement(6954..8270)
                     /locus_tag="IWO17_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0288918.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(8267..10102)
                     /gene="walK"
                     /locus_tag="IWO17_000009"
     CDS             complement(8267..10102)
                     /gene="walK"
                     /locus_tag="IWO17_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ0288919.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(10107..10811)
                     /locus_tag="IWO17_000010"
     CDS             complement(10107..10811)
                     /locus_tag="IWO17_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0288920.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(11001..11861)
                     /locus_tag="IWO17_000011"
     CDS             complement(11001..11861)
                     /locus_tag="IWO17_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0288921.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(11848..12417)
                     /locus_tag="IWO17_000012"
     CDS             complement(11848..12417)
                     /locus_tag="IWO17_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ0288922.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            12798..12870
                     /locus_tag="IWO17_000013"
     tRNA            12798..12870
                     /locus_tag="IWO17_000013"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12831..12833,aa:Lys,seq:ctt)
     gene            complement(13366..13752)
                     /locus_tag="IWO17_000014"
     CDS             complement(13366..13752)
                     /locus_tag="IWO17_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0288923.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(13749..14270)
                     /locus_tag="IWO17_000015"
     CDS             complement(13749..14270)
                     /locus_tag="IWO17_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0288924.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(14272..15306)
                     /gene="sppA"
                     /locus_tag="IWO17_000016"
     CDS             complement(14272..15306)
                     /gene="sppA"
                     /locus_tag="IWO17_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0288925.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(15339..16754)
                     /locus_tag="IWO17_000017"
     CDS             complement(15339..16754)
                     /locus_tag="IWO17_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAQ0288926.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(16723..18876)
                     /locus_tag="IWO17_000018"
     CDS             complement(16723..18876)
                     /locus_tag="IWO17_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAQ0288927.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            19134..19370
                     /locus_tag="IWO17_000019"
     CDS             19134..19370
                     /locus_tag="IWO17_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ0288928.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            19389..19604
                     /locus_tag="IWO17_000020"
     CDS             19389..19604
                     /locus_tag="IWO17_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ0288929.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(20307..21059)
                     /locus_tag="IWO17_000021"
     CDS             complement(20307..21059)
                     /locus_tag="IWO17_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0288930.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(21074..22321)
                     /locus_tag="IWO17_000022"
     CDS             complement(21074..22321)
                     /locus_tag="IWO17_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0288931.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(22397..22543)
                     /locus_tag="IWO17_000023"
     CDS             complement(22397..22543)
                     /locus_tag="IWO17_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ0288932.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(22647..22958)
                     /locus_tag="IWO17_000024"
     CDS             complement(22647..22958)
                     /locus_tag="IWO17_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0288933.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(22960..23157)
                     /locus_tag="IWO17_000025"
     CDS             complement(22960..23157)
                     /locus_tag="IWO17_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ0288934.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(23551..25278)
                     /locus_tag="IWO17_000026"
     CDS             complement(23551..25278)
                     /locus_tag="IWO17_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0288935.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(25271..26464)
                     /locus_tag="IWO17_000027"
     CDS             complement(25271..26464)
                     /locus_tag="IWO17_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0288936.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(26651..27295)
                     /locus_tag="IWO17_000028"
     CDS             complement(26651..27295)
                     /locus_tag="IWO17_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0288937.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(27389..27523)
                     /locus_tag="IWO17_000029"
     CDS             complement(27389..27523)
                     /locus_tag="IWO17_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ0288938.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(27525..29657)
                     /gene="feoB"
                     /locus_tag="IWO17_000030"
     CDS             complement(27525..29657)
                     /gene="feoB"
                     /locus_tag="IWO17_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ0288939.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(29654..30127)
                     /locus_tag="IWO17_000031"
     CDS             complement(29654..30127)
                     /locus_tag="IWO17_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ0288940.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            30528..30752
                     /gene="nrdH"
                     /locus_tag="IWO17_000032"
     CDS             30528..30752
                     /gene="nrdH"
                     /locus_tag="IWO17_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ0288941.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            30911..33070
                     /gene="nrdE"
                     /locus_tag="IWO17_000033"
     CDS             30911..33070
                     /gene="nrdE"
                     /locus_tag="IWO17_000033"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ0288942.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            33120..34085
                     /gene="nrdF"
                     /locus_tag="IWO17_000034"
     CDS             33120..34085
                     /gene="nrdF"
                     /locus_tag="IWO17_000034"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ0288943.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(34174..35313)
                     /locus_tag="IWO17_000035"
     CDS             complement(34174..35313)
                     /locus_tag="IWO17_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0288944.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(35622..36335)
                     /locus_tag="IWO17_000036"
     CDS             complement(35622..36335)
                     /locus_tag="IWO17_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ0288945.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(36589..37290)
                     /gene="nagB"
                     /locus_tag="IWO17_000037"
     CDS             complement(36589..37290)
                     /gene="nagB"
                     /locus_tag="IWO17_000037"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ0288946.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            37524..39056
                     /locus_tag="IWO17_000038"
     CDS             37524..39056
                     /locus_tag="IWO17_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0288947.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(39247..40449)
                     /locus_tag="IWO17_000039"
     CDS             complement(39247..40449)
                     /locus_tag="IWO17_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0288948.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(40531..41220)
                     /locus_tag="IWO17_000040"
     CDS             complement(40531..41220)
                     /locus_tag="IWO17_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0288949.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(41326..41694)
                     /gene="rplL"
                     /locus_tag="IWO17_000041"
     CDS             complement(41326..41694)
                     /gene="rplL"
                     /locus_tag="IWO17_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ0288950.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(41747..42250)
                     /locus_tag="IWO17_000042"
     CDS             complement(41747..42250)
                     /locus_tag="IWO17_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ0288951.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(42660..43349)
                     /gene="rplA"
                     /locus_tag="IWO17_000043"
     CDS             complement(42660..43349)
                     /gene="rplA"
                     /locus_tag="IWO17_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ0288952.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(43453..43875)
                     /gene="rplK"
                     /locus_tag="IWO17_000044"
     CDS             complement(43453..43875)
                     /gene="rplK"
                     /locus_tag="IWO17_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ0288953.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            44218..44886
                     /gene="sdaAB"
                     /locus_tag="IWO17_000045"
     CDS             44218..44886
                     /gene="sdaAB"
                     /locus_tag="IWO17_000045"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ0288954.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            44899..45771
                     /gene="sdaAA"
                     /locus_tag="IWO17_000046"
     CDS             44899..45771
                     /gene="sdaAA"
                     /locus_tag="IWO17_000046"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ0288955.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(45862..46881)
                     /locus_tag="IWO17_000047"
     CDS             complement(45862..46881)
                     /locus_tag="IWO17_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0288956.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(47054..47941)
                     /locus_tag="IWO17_000048"
     CDS             complement(47054..47941)
                     /locus_tag="IWO17_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0288957.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(48018..48563)
                     /gene="nusG"
                     /locus_tag="IWO17_000049"
     CDS             complement(48018..48563)
                     /gene="nusG"
                     /locus_tag="IWO17_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ0288958.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(48664..48834)
                     /gene="secE"
                     /locus_tag="IWO17_000050"
     CDS             complement(48664..48834)
                     /gene="secE"
                     /locus_tag="IWO17_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ0288959.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(48858..49010)
                     /gene="rpmG"
                     /locus_tag="IWO17_000051"
     CDS             complement(48858..49010)
                     /gene="rpmG"
                     /locus_tag="IWO17_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0288960.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            49172..49816
                     /locus_tag="IWO17_000052"
     CDS             49172..49816
                     /locus_tag="IWO17_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0288961.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(49872..50375)
                     /locus_tag="IWO17_000053"
     CDS             complement(49872..50375)
                     /locus_tag="IWO17_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0288962.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(50388..50434)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            50972..51937
                     /locus_tag="IWO17_000054"
     CDS             50972..51937
                     /locus_tag="IWO17_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0288963.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(52036..53208)
                     /locus_tag="IWO17_000055"
     CDS             complement(52036..53208)
                     /locus_tag="IWO17_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0288964.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(53195..54340)
                     /locus_tag="IWO17_000056"
     CDS             complement(53195..54340)
                     /locus_tag="IWO17_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0288965.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(54354..55278)
                     /locus_tag="IWO17_000057"
                     /pseudo
     CDS             complement(54354..55278)
                     /locus_tag="IWO17_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            55395..56474
                     /locus_tag="IWO17_000058"
     CDS             55395..56474
                     /locus_tag="IWO17_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0288966.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            56506..57099
                     /locus_tag="IWO17_000059"
     CDS             56506..57099
                     /locus_tag="IWO17_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0288967.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(57209..57436)
                     /locus_tag="IWO17_000060"
     CDS             complement(57209..57436)
                     /locus_tag="IWO17_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0288968.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(57539..60127)
                     /locus_tag="IWO17_000061"
     CDS             complement(57539..60127)
                     /locus_tag="IWO17_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ0288969.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(60147..60806)
                     /locus_tag="IWO17_000062"
     CDS             complement(60147..60806)
                     /locus_tag="IWO17_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0288970.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(60855..61358)
                     /gene="trmL"
                     /locus_tag="IWO17_000063"
     CDS             complement(60855..61358)
                     /gene="trmL"
                     /locus_tag="IWO17_000063"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ0288971.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(61579..62445)
                     /locus_tag="IWO17_000064"
     CDS             complement(61579..62445)
                     /locus_tag="IWO17_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0288972.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            62554..63195
                     /locus_tag="IWO17_000065"
     CDS             62554..63195
                     /locus_tag="IWO17_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ0288973.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(63257..64618)
                     /gene="mgtE"
                     /locus_tag="IWO17_000066"
     CDS             complement(63257..64618)
                     /gene="mgtE"
                     /locus_tag="IWO17_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ0288974.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(64636..65535)
                     /locus_tag="IWO17_000067"
     CDS             complement(64636..65535)
                     /locus_tag="IWO17_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0288975.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(65537..66334)
                     /locus_tag="IWO17_000068"
     CDS             complement(65537..66334)
                     /locus_tag="IWO17_000068"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ0288976.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(66312..67016)
                     /locus_tag="IWO17_000069"
     CDS             complement(66312..67016)
                     /locus_tag="IWO17_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ0288977.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            67147..67722
                     /locus_tag="IWO17_000070"
     CDS             67147..67722
                     /locus_tag="IWO17_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ0288978.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            67840..68517
                     /locus_tag="IWO17_000071"
     CDS             67840..68517
                     /locus_tag="IWO17_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0288979.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(68596..70404)
                     /gene="pepF"
                     /locus_tag="IWO17_000072"
     CDS             complement(68596..70404)
                     /gene="pepF"
                     /locus_tag="IWO17_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ0288980.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(70580..71578)
                     /locus_tag="IWO17_000073"
     CDS             complement(70580..71578)
                     /locus_tag="IWO17_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ0288981.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(71804..72457)
                     /locus_tag="IWO17_000074"
     CDS             complement(71804..72457)
                     /locus_tag="IWO17_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ0288982.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        21786 a        15013 c        12375 g        23593 t
ORIGIN      
        1 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt tccagcctct ttttatctgt
       61 ctttcactaa tgatcagttg tagataagca agttgctttt tcttacatca taccgcccat
      121 cattgatgga tccattgcag gagctgctgg cgctgctggt tctggtttat ctgcaacgac
      181 tgcttctgtc gttaataaca aggcagaaac ggaagcggca ttttgcaagg cagaacgtgt
      241 tactttagtt gggtctacga tacctgcttc aaccatgttt acccattctc cagtagcagc
      301 attgaaacca gtgcctagtt ccacgttctt caatttatca acaatcactg atccttcata
      361 accagcgttt tcagcgattt gacgaattgg ttcttctaaa gcacgaacaa cgatcttgat
      421 acctgttgcc acgtctcctt ctgcttctac agcagaaact ttgctgataa cgttcactag
      481 agctgtacca ccacctgaga ccattccttc ttctactgcg gcacgtgtag cattcaatgc
      541 atcttcaatg cgtaatttta attctttcaa ttctgtttca gtagctgcac caactttaac
      601 aacagctaca ccacctgcta atttagctaa gcgttcttgt aatttttcgc ggtcaaaatc
      661 agaagttgtt tcagcaattt ggtttttgat caattgcaca cgagcttcga ttgcttcttt
      721 ttctccagaa ccttctacga tcgttgtatt gtctttatct acaacaactt tagaagcatt
      781 tcctaagttt tcgattgttg catctttcaa ttctaaacct agatcgtcag taattactgt
      841 accacctgtc aaaatagcga tatcttcaag cattgcttta cgacgatcgc cgaaaccagg
      901 agcttttaca gctactacgt tgaatgttcc acggattttg ttcaatacaa gagttggcaa
      961 tgcttcacca tctacatcgt cagcaataat caacaatgga cgtgattgtt gcaagatttg
     1021 ttctaacaat ggcagaatat cttggatgtt agagattttt ttgtctgtga tcaagatata
     1081 tgggttttct agaacagctt ccattttgtc gttatctgtt accatatatt gtgataagta
     1141 accacgatca aactgcatac cttctacaac atctagttct gtttcgatcc cttttgattc
     1201 ttcgattgtg atgacaccgt cgtttccaac tttttccatt gcatcagcaa tcaaatgtcc
     1261 aactttatca gaacccgaag aaacagctgc tacttgtgca atggcttctt ttgaatcaac
     1321 aacagttgag atattatgca attcttcgac tgctgctttt gttgccaatt cgatcccgcg
     1381 acgaatgcct agaggattag cacctgcagt tacgtttttt aaaccttcgc gaacgattgc
     1441 ttgtgtcaga acagttgctg tcgttgtacc gtctcctgca atatcatttg tttttgatgc
     1501 tacttctgat acaagttttg cacccatgtt ttcaaaatga tcttctaatt cgatttcttt
     1561 tgcgatcgtc acaccatcat ttgtgatcaa tggagagccg taagattttt ctagtacgac
     1621 atttcggcct ttaggtccta atgttacttt cactgtatct gctaatttat ctaccccgcg
     1681 taacatagct gcgcgtgcgt cttctgcaaa tttcaattct tttgccatga ttgattcacc
     1741 tcattgatta tttttgatta tttttttatc ttccaaattt actcaacaat cgccatgata
     1801 tcttttcctg cgacgatcag atattctttt ccttcgtatt ttacttctgt accggcgtat
     1861 ttttcgaaca taacttggtc gcctgctttt acagcggcag gaactttttc accgttttct
     1921 aacaaacggc cttcaccgac tgcaacaact gtcccagttt ggggtttctc tttagcagca
     1981 gaagctaaaa cgattcctcc gacagttttt tcttcttctt ttgcgacttc gataatgacg
     2041 cgatcaccta atggttttaa cacgataaat ccctccgata actctttttt taatctgtta
     2101 gcactcttaa ccttcgagtg ctaacctaca tttttaataa tactcattct ccctcttttt
     2161 tgcaagcttt taaatcaaag aaaagaaatt ttattctgac agctgcttat tgaaattatg
     2221 atataattga aatcaatcga tataagcttg ccttgcatgc ttgttatccc ttaaaaggag
     2281 gaattacatg tctttaaaaa aatatagctt cctcagcatt ttttgctatg gtctaatttt
     2341 tatctcaccc ttgatcctag cttcgatagg acttgtaaaa tctacgtctg aattgatcac
     2401 agcaactgca gtcatgtata tactaggcgc cgttgtccta gctatttttt attttaagca
     2461 acgtgaaccc ttagcgatcg aatcagcggt aaaaaaagct tctgctccaa agattgttat
     2521 ttatggattg gttggaatct ttgtcgcatt gattctgcaa agcatcgctg tcatgttaga
     2581 atctgtattg tttggcaaag caaccccttc cgagaataca caaaatatca tccagatgat
     2641 catggaagca cccgctttta ttttggcgac aactgttgct ggacccatca tggaagagtt
     2701 cgtattccgc cgcagtatac tggggatcat cagtcgatat tcaaattttt gggtcggagc
     2761 agtgatcagc tctttgctgt ttgcgtttgc tcataatgat ggtcatctgc taatctactt
     2821 tttcctaggt ttcttcttca gcctagagta caaagcaact ggtcggatct ggacatcgat
     2881 gatcacacac gtaggaatga acacattagt tgttcttgtt caattagctg tccaaaaagg
     2941 gctagtataa acatgactat aaatactcct gtcatcctag attggtgatt ttcccaatct
     3001 aaagcgacag gagtattttt gttttttagt cgttcatctt ttctgctgct ccatcatttt
     3061 ttgtctgtct ttcctttttg cagtttgcag aatctatttt tttcaaaaat gggaaataaa
     3121 tgaaaaaact aattgacaac acgatacctt gtaaaatcgc tgcaggcact ccgcctacta
     3181 agaaacccga gaggatcggc ggtgttgtcc aagggacttg gactgctgta aataaaggaa
     3241 cgaatccaaa atataaagcg gaataggtaa tcaatcctga aacgaccggt gcgaaaataa
     3301 aaggaacagc catcatcggg ttcatcacga tcggtgtgga aaaaataatt gattcattga
     3361 tgttgaaaaa ggctggtaaa agagcaagat ttcctaattc tctgtatttc ttcgatttcg
     3421 caaaaaccgt cataaaaaat acagccccta acgtcaatcc agcacccgta accgtcatga
     3481 actgatcgag aaattgctga gtaacgatat gtccgccatt tgccaccgtc aactcttgtc
     3541 ctgaggctaa gatttctgca tttgctaatg aattcgcttg caagacggga ctaaccaacc
     3601 cattgaccat cgcagatcca tgaagaccaa ataaccataa aaacggaaca aaaaaaccta
     3661 ataaaagcac accaaacaac gtgtctatga acgattgaat gggaagctgg acatatgtcc
     3721 atactccttg aacaacagtc attctgaaca aatgttgaaa aaccgctgaa ccgataagcg
     3781 taaaagtgac tataacaatg attggaatca atgaagtaaa agactgccaa acattttcag
     3841 gtatcttctc tctttgcttc caccctttcg caagtcttac taaacgtgca tagccccatc
     3901 cgatgatgag cccagttgta actgctccta gtatcccttc agcacctgtc cagacagtat
     3961 ctatctgagt atttagtggc tgaataagca gtagataagc agacaaggct gttatgccga
     4021 ctggtaagcg atcgatgtct tcttcttttg cataagcgta tgcaatggca aaaacgatcc
     4081 aaaaggctat cacatcaaat gtagcctggt atacttgtgt aaaatacata gaaaatcctg
     4141 ttttgtctat ccactgagcg atgtaatcca caggaaaatg agcaaataag aaaaacagcg
     4201 agccaacaat aattattggg atcgtgtaag tcattccatt acgcaaagca gttaagggtc
     4261 taagattcac aacagatgtt atcattggta aaaaggcacg atctaaccac tctttcattt
     4321 cttatctcct tcaacttcta tttttatcaa tcagttttct taatttaact atacccaatg
     4381 atcacgaacc atgaaaggaa tgcgcactga gcttttcaaa aatcggattc tttgacgaat
     4441 attcagcaac tgcttacaat ggacaggaat aaaaaaggag gttttttatg aaaattgcac
     4501 tcattggagc tactggccat gcaggttcat ttattttaga cgaagcattg tccagagatt
     4561 tagacgtgac ggcaatcgtg cgtcatccag aaaaattgcc atcagatgtt ccgtttatac
     4621 aaaaggattt atttgagttg acaactactg atcttgcatc ttttgatgtt gtcgttgatg
     4681 ctttcaacgc tcccaaagga aaagaagaaa tgcatcaaac ttctttgtcc catttgattg
     4741 agatgcttga aggaaccgat acacgcttaa ttgtcgttgg gggtgcctct tcgttattct
     4801 tagatacaga aaagacgact cgtatgattg accaagtacc taaaaatgca cccttctatc
     4861 caacagcttt caatatgtac gaaggattgt taaaattgaa acaatctaaa aatctacatt
     4921 ggacttatat cagtccagct tctttatttg atccacatgg caaacgtaca ggtacttatt
     4981 tgttaagtga tgattattta caaagaaacg ctgctagcga cagtacgatc agcatggcag
     5041 attacgcgat tgcattagta gatgagatac tggatgagaa acatgaaaag cagcacatca
     5101 gtgtagtcag tcgataatac ggttattttt ttaatatgct aaaaaaataa aaatagaaga
     5161 taaaaccaac cctgattgat tttatcttct gctttttata tttttattga ttgaactgtt
     5221 taaatcgaaa atagctcagt tgcttcatct ggatctgtat catagatttc aaaatcatgt
     5281 ccaacgccaa aatctttttc tttcaaaaca ttttccattg ttaaatgagc aagttctttc
     5341 atattgtttt ccttgctaag atggccaaga tagattcgtt tggtccgatt accgatcaca
     5401 tcagtcatga caagtgcacc gtcttcgttg gaaagatgtc ctctatctcc taaaatacgc
     5461 tgttttaaat tccacggata aggtcccatg cgtaacatct caagatcatg attactttcc
     5521 atcaaataac catcggcatt ttcaatgaca cctcgcatat aatcactgca atatccggtg
     5581 tccgtcaaca tgacgaatga ccgattttct ttatggaaac gataaaattg cggagccgct
     5641 gcgtcatggg agacgccgaa actctcaata tcgatatcgc cgaatgttct gactttcccc
     5701 atctcaaata gatgtttttg ttccactggt acatttccaa tcagtggttc catcgcttcc
     5761 caagtctgct gattggcaaa aacatccaaa tgatacttac gagccaagac acccacgcca
     5821 tgtatatgat ctcgatgttc atgcgtcaca agtattccat ctaattggtc cggtgtccga
     5881 tcaatttgag ccatcaagga agtgattttt ttaccgctaa gtcctgcatc gaccaaaagc
     5941 cgtttttttt cactttcgat ataaagtgaa ttacccgaac ttccacttgc taggatactg
     6001 attttaaaag ctggtttttc ttcagccatt tgactcctac ttcccttctt actttgtaaa
     6061 cttttttcat tatactcgct tttttattga tcttccactt ttggtacagt attgtttgtc
     6121 acgatcgtat tgctgaaagc gttgacgctc tcaatttgga ccactttatc gcttgtttcg
     6181 atcgctacat gccaaacagg aacatacaca tttttttccc ggactttcag aatacaggaa
     6241 taagctagct gacgccataa aatttttgat cctcttggta ttttgttatt gatgtacaat
     6301 gtattgattg cttcctcttc cgtatacagt tccgtctttt cgcgcagctg ctcgatatca
     6361 gataaatgcg tctgcatgta tttcgtgatg ctataatcat tatcgttttt ttctaaagaa
     6421 agaacgattt ttgctgtatc atcattgaat gggatgcctt catactcttg gcttgcttgg
     6481 atttctagtg gttcttcttc taaatgagaa aagtttttcg tgtacacata atcattaccg
     6541 aataaaatat tgtctttgtt agataaaaaa tcttccaagg aactactaat tttatctgga
     6601 tcgatcacgt tcttttcagc atctgaactg gataaagatt tcgtcaaaac atcaccgtct
     6661 aaaaacacac cacttgccag caagccgctt ttaccagcct tgcgataatt cgctaatgaa
     6721 gtgctaagat tggtctgctc cgcgctaaga taatagcctt gcttgttctc agacgaaatc
     6781 ttccccgtgt aagtgatatc gtcgctagcc agacgttgtt ggattgattc agtctgatta
     6841 gagcgaatga cattgttttc ttggtatacc gcgtcatgat aactgctgaa taaaaacaca
     6901 ttcaacccta aaaaagcgac gaaaaatatc cattcgattt ttttgaaatc caattaattc
     6961 gcctccaatc caggtaattt ttccagaagg tcgttagctg aataccagtt atctttatat
     7021 ttcacatacc attcaggtaa gagatcaacg acacgattcg tttctttgac attttgccat
     7081 gtatagccaa cgatcactga accaatcttg ttgctatctg ctcccttagc ttttaactgc
     7141 tggatgattt cattcgtact tgcaagttct acttcttcgt ctgaaggtat cggcacttga
     7201 atcgtattca aactggtttg gatattcact tgcacagaac cattcgtttc accgccaact
     7261 tccacaccaa tctgtcctct gctgtctgtc ccaaaaaccg ggaagccttc cacgaatatc
     7321 cgataatcga tcgtattgtc actgcggtca aaatatctga ggttccctaa agaactgcct
     7381 aatttgctaa tataggggaa actttcagaa aatacactag cattttcttt tttctctggc
     7441 agctctccac gaaaatcaac tacttgcgtt tccgaatgga tagacattgt ttcattccca
     7501 ccgtaaaaaa tcaattcatt gctttcatca ttatttttca cgctatctgg tttctggaaa
     7561 aaagcatttc taaaaagtgt atagggttgc tgtgacagga tataactata cttcttcagt
     7621 ttgatcgaat gtttcgtagc atattgcgtg tcgatcgtcg atggttctgt tgtcatttct
     7681 gtccattttg tgtcttttgc tgttaattct ttattgatat ctgtccaatt caacgaaata
     7741 tttgcttcat atacgagatg gttcgaataa ttcaaaaatc ggattttttt atttccttga
     7801 tcgaattgta ttctagtaaa atacatattt tcgctttctt ttgccaagtt cgacacatct
     7861 aaatctaaat caaatgcttc cttgtattct cttagcaaaa aaggcgcatt ataggccaac
     7921 tcaaaaccag agttcagatt tttcacctgt tcaaactgtg atttgtctcc tgaaactact
     7981 tcagaaagct ttccaaatct tgctttatcg atgatcaatt gcaatcggta cacgagattt
     8041 tcactgttgg tttcttggat cattcccgaa tggtgccatg taacatgaag cggaagaaag
     8101 acttcggaag tttttcgatt gctttgagac tcgatcattt gagtgtttgt ctcatctacg
     8161 ttgattgaac tttttcctgc tgggctcagc cagattgcat aagagaagta tatgcttaac
     8221 gcaaccatca aaatgagtcc tatacgaacg actttttcag agattctcat tcccaccatt
     8281 cctcctcata aggttcatat ggcagcgaga tataaaaagt cgaccctttt ccttccttgc
     8341 tctctgccca aatagcgcct cgatgcgcct taatcacttc tctagaaata gctaatccaa
     8401 gtcctgtccc accttgtgct cttgcacgtg ctttatctac tcgatagaac cgctcgaaca
     8461 ctcggttcag atctttttta ggaatcccca atccttgatc ggtgatactt aaaatcacgt
     8521 tattatgcgt ttctaataag cgacaagtaa tcgttccccc atctggtgaa tacttgatcg
     8581 cgttattcat gatattatcg actacttgga tgattttatc tgtatctact tcgacccaaa
     8641 gatctctttg cgtaaattca cgacggatct tataattttt ttcctgattt cccaccatca
     8701 tatcaaaccg gtcaagaaca aaattcacta actcgttgaa attgacatat tctaattgaa
     8761 gctgggtatt acctgtatcc atgcgagata gatttaagag gtcattgatc attcgaatca
     8821 ttcggtctgt ttcatctagc gttaccttca aaaaatttgg tgctacttcc tcgtctttcc
     8881 acgcaccttc gctcaatgct tcgatataac tacgcatact tgtcaacggt gtacgaagtt
     8941 catgggaaac attagagacg aattcacgac gttcccgttc tgttttttct tgttctgtca
     9001 catcatgcaa gacagctact aatccgctga taaaacctga ttcgcgacgg atcatagaaa
     9061 agtctactcg caagatcatc tgatcacttt ccaatgtact ggttgaacgg tcaagtacca
     9121 tttcgtttgg atcttctaat aatttccgca acgtatattc ttcttcgata tctaaaagtg
     9181 tcaaaatcga ttgtccgatt gcttcctcgt ttttgatatc tagcaaggaa acagccatat
     9241 cattgatcgt aatgacttta cccctgcgat ctgtcgcaat gacgccgtct gtcatatggg
     9301 atagcacgct atccaaacga ttgcgttcag cttccattgt atcttgagct tcttcaatac
     9361 gttcagctaa ctgattgaag gtatcagcca gctgtccaag ttcatcttgt ccatgcactt
     9421 tgactttccg actgtagtct cctctagcga ttcgaatggc ttgttctctc atttcaccga
     9481 tcggctgtgt aatcgaacga gcaactaaaa cagatacgat cattgaaata aatgccgcaa
     9541 ttagtgaggc agtaaagaaa atgactgctg tgttattgat ttccgaatat ttttgttcga
     9601 tattactttt gacgtaaaga gccccgataa cggcatcgcc ggtcggggac aggatcggtt
     9661 gaacattgat attcacccgt ttatcgtcgt tatctaaagc aacataacgt ttagtcgtga
     9721 aatcattgat atcaacataa tcatttttct ttcccacagc gctttgttca ttgacatcgg
     9781 ttgttcctcg gatgatccct ttttcgtcaa cgacccgtat ttcgatgata tcgctagtcg
     9841 cactgttttc aataaatctt ttgatattcg catttacctc ttcatcatct gcattatctt
     9901 ttcgattcaa ttccgggctg attgttgttg ctaatgattc caccgtttga ttcatatctt
     9961 taataaatgt attgatcgtg gaacgttcta aaccacgtat aaagtaggca ccaataattt
    10021 cgatcgagat caataaaatc agaataaagg taatagcgat cttaaaattg actgatcgaa
    10081 aaaagcggac ttttcttttc atccaattac tcctgttctg ggttacgcaa atagtaaccg
    10141 acaccacgac gtgtcactaa ataatttgga tggcttggat tatcttcgat cttttcacgc
    10201 aaacgacgta cagttacatc cactgttcgt acatctccaa aataatcata accccaaacc
    10261 gtttgtaata aatgttctct tgtcatcact tgtccaaggt gcttcgccaa gtaaaagagt
    10321 agttcgaatt cacgatgtgt taactcgatt gtttctcctc gtttcgtcac catgtaagct
    10381 tctggatgga tcgtcaaatc accaatcgtc aattccgcag gtgctgcctc ttctggttcc
    10441 ttcgcagccg tggcaccgcg acgaagattt gctttgacac gtgcaactaa ttctcggttg
    10501 gaaaatggct tggtcacgta atcatcggcg cctaactcta agcctaggac tttatcaatt
    10561 tcagaatctt tggctgttac cataatgatc ggcatatcat aagttttgcg tacctcacga
    10621 gcaacttcta agccgtccat tttaggtagc atcaaatcaa gtaaaatcaa atcaggttct
    10681 acctcagcaa ctttttccaa ggcttcttcc ccatcatatg cagtataaac gtcatagcct
    10741 tctttggcta aattgaattt gacgatatcc gaaatcggct tctcatcatc gacaactaaa
    10801 acttttttca taaagagcca cctcttatat attattgtca taccttctca ttataccgta
    10861 tttctgcttt tgtttcatct ttgcctataa aacaaaagag aaaattaaaa tgaaagtgca
    10921 aaatacttta ttattttttg ttctaacttc aataaaaaag gaaaacactg attttcacct
    10981 cagtgtttcc tttttttgat ttatccttct aaaacttgta caccgacacc caataatccc
    11041 ggaccagtgt ggactactaa tgccggagaa atctggccaa agtagatttg gtttgcttgt
    11101 gggaactctc ttttaagtct cgcttccatt tcaattgctt cttctttcgc atccccatgg
    11161 gcaacagcta agttgaagac ctttgcatta ccgatttttt ctttgacgta agcaaccgtt
    11221 ttatccaaac tttttttacg tcccctagct tttcctactg tgtaataaat cccttcttca
    11281 ttacatgaga tgattgggtt aagctttaaa gcggttccta caatagaagc aactaaaccg
    11341 atccgtccac ctttttgaag atattccaat gtcgcaacat taaagaaaac ttttgttctt
    11401 tcgactaact cattcagatt actgatcagt tgatcccatt ctactccttc ttccagccat
    11461 tttgctgctt ggatcgcaga aaaacctgca ccaataccaa tatttttcgt atctagtaca
    11521 tatgcttcca attcttcttg ctgttgtgca attaaacgaa ccacattata tgtcccgctc
    11581 aatccactgg aaattgttac cgcaagtacc ttttcataac cgtctttttt gatttgctca
    11641 aagatttctg tgatcgactc accatccggc aaagatgtac cagggatttc ctctggcaaa
    11701 cgttgataga tctcttcagg ggtaatgtca attttatctg tatatacctg atctttataa
    11761 ataattttca atgggatcat atacatctcg tattttttta tcaattcttc tggaacatcc
    11821 gtacctgaat ctactaaaat tgcgatttta ttgtttttca tttgttttct cctcttttaa
    11881 atattcggtt tctagaacaa tcgttttttc cgcaagaagt ttcattgcaa acgataaagt
    11941 tgccgctctt acagcaatat attctacagg ggttgcctgg tcgaattttt gcggatgaga
    12001 tttaccttgt gctaattggc tgaccatcca cacagctgct tcttgttcat cgcaaaatag
    12061 attataggct tcacgaattc ctactgtttt tccttggaac aatatccctt ctttgatttc
    12121 aggaatagtc aatacttgtt tcaacgtagt gattgcaagt aaaaatgcta cgtgttcttt
    12181 gttatatctt tttttaactg gtgctggtat caaacctagt ttaacgtaat tgtttaccat
    12241 agaagaggta agcaatggat gcttttctcc ttgtatcaca ggtgaaagat aacgatcaac
    12301 taacgttctg acttggtcca tataaagttc taagtcaggg agttcttccc agtgaggcag
    12361 acggaatgtt tctagttcct tcccccactc cattaattgc tgacgcattt tttccatcat
    12421 aaccgctcct tttttcattt cataaacatg atatcagact tatgataaac attcaatcta
    12481 gttgctttaa ctaggtgatt tgattagcgg gattccatga aaacaaaaaa caagaaggct
    12541 aagccttctt gtcttttatc gttaagaaca ggagacccgc cggggagacg gcgggaaagg
    12601 agtaaaaaat gaaaaaagtg ttagggtttg ttctcttgca agaactttgt ttgttggtat
    12661 gcttatataa tagcgagcaa atgtgaataa tctatgaatg atttcctata aggaagtaaa
    12721 gatttttctg attttcttta aatgtagtct tgcattttaa aattcttagt gctactatta
    12781 ttaagtactt tttgtacggg ccgttagctc agttggtaga gcagctgact cttaatcagc
    12841 gggtcgcggg ttcgagcccc tcacggccca ttgggtgcca aacccacgga acaggtaaat
    12901 gttggtgcga aagcatttcg aaaacattta cgctgttctt tttttatttt tcaaaataac
    12961 tagggctttt tatttttaac tcaaagtcca ctgtagccct ttggactttg agttaaaaaa
    13021 gaagcataat tatgaacagt aaagtttcta atataacaat aattcgagaa gctttgcaaa
    13081 atcatgctgc caatgaactc tctaaacata taggattaaa tctaagtact ataaaaggtt
    13141 aaaaagtgaa gaatatttaa tagaaaaact aaatttactt gctgctttcc ccttttccat
    13201 taatacaaca atcagagagt gtcttttttt cgaaaaaatt agaataaata aaagacacgt
    13261 agttttttta tctacatgtc ttccatcgac tacaagttat catttctcaa aacgtattta
    13321 gcttttaatt gattaatcgt ttcacgttgt ctacacattg cctaattaag attttcttat
    13381 tagaagcatc aatcccgcaa taatacctaa aactccagga attacccaca ataatgttaa
    13441 aagaaaaaat ggaataatag ataaaatgag taaactaata ccatagacca ctgttttttg
    13501 attttttctt actaaaaaac ctattatgaa aatcattatc gaaaaaacta gggtaattcc
    13561 tccaaataaa acaacattcc cgcttagatt gactaacata tctatatcct tactggttag
    13621 ttctgtagta tttaaagtag ttgaatcaat agaagataga aacataccac ccatcaatgc
    13681 taaaagacta cataaaatcc ctgttattcc actaattaaa atcaaaataa actctgtttt
    13741 tctcttattc atttaattct ctccagatta agtgttttag aattagaaat tttttgacca
    13801 tagttaatag cttctaatga acgaataaca accactgacg tgttagaaaa ataatcccca
    13861 atatgttgtt tcttgggcgt gaaagcagcc accaaatacc caatttgaaa tacaaaattt
    13921 tttaatataa acctacatat acactcacga ataaagatag tttgccaact tagctctttt
    13981 tcctcaaagc tcactactct tatcccaaaa atcattttcc caattgtttg tcctgaattc
    14041 agcttagtca aaagtacaaa ataggttaaa taaatagcta atgacgctat accataaaaa
    14101 gtaataatgg atttgtcaga ttccagtccc atgattttgt aaaagagacc tatagtagca
    14161 tgggtgatga tgctaataca aattaaatca ataagaaaag caaaaaatcg taaccaaaat
    14221 cccgcaaata cataattagg caaagtttca atgccactct tcttattcat tttatagccc
    14281 tccatagtaa tacattggtt ttggagtatc agaaaatcca agtttttcaa aaactgaaat
    14341 tacacgatct gatgtagttg cctttagccc ttgccattca gcaatcttat tacctaacca
    14401 agaagaagca aagccagttg tgtcattttt atattctata accgtcgcat cagttaattt
    14461 cttctctttt tttagggaat ctaatgcatc ttcagagtaa ccaatttgat ccaccaaccc
    14521 attttctact gcttggttac catcataaat tcttccatcc gcaagttctt tcactgcatt
    14581 agtatccata tttcttcctt ttgctacaac attgacaaaa cgatcataag cactcatcac
    14641 aaattcctgt ataactttat tctcttcctc tgaaggtttg tgttgaggtg acatcatatc
    14701 ttttaatgca ccacttttat acgtcgcatc agttacgcca agtttttcta ataagccaga
    14761 ataatttaaa ccagaaataa tgacaccaat tgatccagtt gttgtttctt ccgtagcaaa
    14821 tattttatct gaaccagcag aaatgtagta ccctccactt gccgctgtat ttttaaatgc
    14881 agtatagata ggaatatcta gctttttaat ttttgccatt tctttagcaa tctcagcact
    14941 ttcatatatt cctcccccag gtgaatttac ttctaataac actcccttaa ccgctttgtc
    15001 ttcttgaatt ttctttagct gtgttaaaaa attctgatgg ttatattgtt ccctactaaa
    15061 aagattgctt tcacctgtat ctgcaataac tccattgacg gatagtttga caatcttttt
    15121 atttgatgcg ccttcttcta acgtttcttc tgtgatttcg ttagatccgt aaaaaatctt
    15181 atttattcct gttagggttg tatttgatgc ttcctcttta tcttttcctg gaattagcgc
    15241 gacgataatg gatagtaaca ataatccgca cgctactccc acagcaatcc atcttctttt
    15301 attcatataa actcctccta tattcttagt aaaaataatt attttttatt tagaaacaca
    15361 tcgacaattt gttgccaata tgttttttct cctacaatta aagacaactc tccagttaat
    15421 ccataacgaa acggaaacga ttgagttgtt tttaacgttc ctttcactgc ataaaaaaca
    15481 cctttctcag tggcagtaga attttctgaa atttcatcta aagttccgtc gatcgtaatc
    15541 ggagccccac ctttttgatc caatttaaaa tgaaccttca tcccttcttt aatataggta
    15601 ctttcattcg caggtaaaag tgctgtaaat ttcatttttg atttttcgtg gatcggataa
    15661 atttctgcaa gcactgttcc tttggctagt tctgcttgtc cctttgtttc ttcatttaaa
    15721 tgaaccgttc cagatacagg agcttttagt attccttttg aaagttgatc gtccaaactt
    15781 ttgataactg actggttctt ctcttgtttt tcatttagct ctgtaactgt ttgttttaca
    15841 gttgttattg cttgttctag ctcttgcttt cctttttccg tttgactaat tctgtcattc
    15901 tcagaagtag ttggtttaac taatttagct ttttctgact gatactgttc tacttctttt
    15961 tttagttgct caatttgttc atttatgctt gctgaaattg tcagtttcac ttgatttttt
    16021 tgatcatcag aaacattatt tagttgttct tgccaattct catagtttgc catcatttct
    16081 tttgaaaaat cttttaatga ttgattatta ctccaagcta cctgtacttg ttgccattct
    16141 tgtaatttac tatctgaact ctcaatttgt ttttctagtt gtctttttgt cttttcgtat
    16201 acttctaatt gcttttgatc atttaattca ctttgcttca aagcgtagcg gagtgattct
    16261 ttttctgaca acatgctctt taattgatta ctgtagccaa aactgtcatc cgttgaaaac
    16321 aaattttttc ctttacttat actttcaata aatagctgag ccatctttat ttgttgagta
    16381 atacgttggt tttcttgttc tatctgagcc ttttcattct ctattaaaga acaatccaat
    16441 ctaaccaaca catcgtcctt atgaacaaat tgattttctt ttaggaagtt ttccattatt
    16501 ttagcttcta atggcacttg taacttctct attttttcgg ggttcaattg agccgttgtc
    16561 cgaataacaa cctctttttt agcaaaggtt aaaaacaatc caataataca taaaaaaaga
    16621 attacagggt ataacaccca acggtagaag ctatgatgtt gtttagtata gatactagaa
    16681 tgttcgaacc aattattatt ctgcataaat tgtttccctc tcctagattt cccataattt
    16741 ttgatagata ccatcggcaa aacgaagttc atcgtgcgtt cctttttcta ccaaatgccc
    16801 ttcatggaga actaaaattt gatcacatgc ttttgctatt ggtaaatgat gcgcaataaa
    16861 aataatcgtt ttttctttta aattgaggag ataattcata attgcatgtt ctaacaaggt
    16921 atctaatcca ctagttgctt catctaaaat taatatgtcc ggatttttta ataaagcccg
    16981 tgctaaggct aatcgttgtc gttgccctcc tgaaagatta tttccacctt cttccaacaa
    17041 tgtctcgtaa cgtaatggct gttgattaat aaattcattt agttggacta tctcacaaac
    17101 ttctattatt tgttcgaaac ttggtgggct atctaaaccg aatataagat tctcaatgat
    17161 tgttccacta aagaaaaatg actcttgagg caaatatgtc actcgatcgc gtaatatctg
    17221 aaaaggaatg tctaagtgat taatgtttcc ataatggatc gatccttctg atgcttcata
    17281 aaaacgaaca aataactttg caagagttga ttttccagaa ccactaactc caactatagc
    17341 cgttttactg tgcgaaggaa ttgtacatga aatttcgttc aacgttgggg ccttcatgct
    17401 atacgagaat gaaacgtttt ttacagtgat atcttgttga aataattgtt cagaaagcat
    17461 atattcaaaa gactggttcg atttttctga atcaatcgcg aaaatttcat ttaaacgatt
    17521 atttgcaact tgagccgttt gcattttcac ttgtaaatta ataatgtttt gtaaaggatc
    17581 tgtcaaaaat actagtaaag cattgtatgt gataagttga cctagactaa ttctattatc
    17641 cataacaaaa taagaaccta gccataaaat taatgcactg ctaattaatt gaatcaaatg
    17701 tttaattcct tgttgaatat tatcaagcgt aaccgtacga aatgattttt tcattaattt
    17761 aacaaattca tggtctatac ggttatatac tttctcttca ccgctatatg ctttaattgt
    17821 ttctattcct ttaagacttt ctatgatact agaatttaca gctgcaccgg ctgccatttc
    17881 ttccctgttc gatttttcat agcttttcac gaaagcgaat attgcaacta cataaaaagg
    17941 caaagagcat agagtgatta aaaacaacgt actattctga atagctaacg taaccccaac
    18001 aagcaacacc ataccaatat ctaaaaatat tgataacgta gcacttgcta aagcatcaat
    18061 aatcttactg gcatcaagaa aacgagaaat aatttcccct gattttctcg tagcaaaaaa
    18121 attcattggt aaagttaaca catgtttgaa atactgaagc ataattgcca tactcattcg
    18181 ctgtcctaaa ataataagaa gataactacg actatattca aacaataccc gaaataaata
    18241 gacagcgatc aagccaatgg aaattatatt taaagttgaa cgtgcttgat tagggatgaa
    18301 aaaatcaaga ataccttgaa aataatatga gcttcctatg ccaaaaaacg taatgaaaat
    18361 cgatgctaaa acgatgtgga atactaatcc tttttgtttc caaattatag gcaaaaaaga
    18421 acttaatcca gctacttttt ctttaatagg aatataagtt tctctaggca taggtaataa
    18481 aagaatacct gtccattctt tttgaaacgc ttcaatcgtt tttctatttt ttccattagc
    18541 gggatctgca atccatagta cgtttccctt cgttttataa attacaacgt agtgcatata
    18601 gctgttatca accaatatat gtgcaattaa tggtaagata agatctcttt ctttccagat
    18661 ctcactatct gcttgaatag ccatacaatc aaaagacaat ttttcaaatg tctttttcaa
    18721 tccaaatgct gaagtaccct ctaaatcagt cccagatagg tcccgtaatt tatgaatcgg
    18781 gatatcagtt ttaaaatatt ttaaaatcat cgaaaggcaa gcaacaccac aatctttttc
    18841 atcttgttgc tgcacaaaag gaaatgattt tatcacttat ctaacacaac tccttctaaa
    18901 aaatttttat agaaaaattc tcaatatagc tttaatacag tgaaaatatt gtctcataat
    18961 tccacaaaaa ctttgaaaac agttcttgaa cactaacttt ttcattcctg aatcaaattt
    19021 attttggaaa ttttactatt caagaataaa cttgataagt ttcaggaaga tttttactgt
    19081 taatcaaata cgtatgttac agtagcaccg taaaaaatat tatttaaaga agcgtgttta
    19141 aacatgcaaa atgtaaagga actaagtatt gtggaaatgc acaaactgtt ggtggaggca
    19201 ataatgcttg gggaaaattg gggcaagttg taggtggact taccacaggg gcagtaggtg
    19261 gtgcaggttt aggaacagca atttgcggac cagcttgcgg tgttgttgga gggttatatg
    19321 gagctgtcgc tggaggtgct gcagctggct gggatgctcg aaaaaaataa aaaaggagaa
    19381 taagtagtat gcatattaaa aacacaaaaa caacatttat attatcttca gaagagttga
    19441 aaaatattca aggtgggagt gccgtgggag ttttaggcac tactttttct ggggcaacag
    19501 caggagtaaa actttgttca gccggaggac cttatgcaat agctgcttgt ggtgtaggtg
    19561 gagctttatt aggtgctgga ttcagcatgt ggaccggtgc gtaaacaatt aaaaaatatc
    19621 aaacctcaag attggtttgt gtcgattacg aacataggta tgttctttat cttttcaaaa
    19681 gtttggaatg gacagaactt tttactaaag cttcttgtgc tagtttttat aattatatta
    19741 aatttttatc ttttattaaa gtcagataaa aattctttgg aaatgtaatg gtagagatat
    19801 ttttacaaaa ggacttgcag cgctgtagtt ggtgctgcag gaggacctat ttcggccgtt
    19861 acaggaggta ttggtaatgt acttgtcgga tgtgcattaa agcctgttcc tgcaagttaa
    19921 atttataaaa acaggaaagt aattatctca aatcctatca agtacaatca taggattgca
    19981 cttgacagga caaatttttt aactaaaaaa tgaaaaacac tttctaaaaa ttagtagaat
    20041 ttgccaaatc tttcgcatct tccaccatac atttaatttt aattcccctt tatggtactt
    20101 aataaaaaca agaaaagaac tattcatttg gccctttttt ggttccagac catagaaata
    20161 ttcctccagt aactaatatt gctgttatgc acgtaactaa taacagctat aaaaattggt
    20221 acaactttaa taggattcag aaaaatttgc actagtgaag gaatctattt tattagaaaa
    20281 atgcaatctc cctaaagaaa aaaccactaa acagtgcttt ctttcaactt ctccttaatt
    20341 ttttgtgctt tccctaaagt aaagtttctg gccaactctg aatcaaaata tagtgaacgt
    20401 tttttaaaat ctatttcttt aacgttaaag atatttacta aacacgctcg actaattctt
    20461 gttaacatag gatatttctt ttcaagctca ctaattcttc cataaaattc gtactgccca
    20521 tctttagtac acaatgataa tctatgtggt aattttgaag cttctacaaa ataaatgtca
    20581 tttatatcaa aagtaaatgt ttgagaacct atggaaaaaa caaatgcttg attcgtaacc
    20641 tgttttgttg catctatcct ttcttgcgct aataacaata actcaacaat ctcgtttcga
    20701 tactcatcta gtgtttgatc ttttgttaca aatcctaacg tttcaactct tctttttatt
    20761 gttacgggta acatctcatc atgagtagtt gtaaaaatga tttttgcttg aacatcatat
    20821 tttctgatta cttctgctaa ttctataccg ttaacttcat gatttaaatc aatatctaag
    20881 aaatatattc cttgctttgg tcggaattgt ttgaggtatt ttttaacttc taacggactt
    20941 tgtgttttca atactatctt aaatacttta tcgtgaaaaa gaatgaaatt atcaataatt
    21001 cgttctaact gatttaattg tataatttga tcttcacata atattattgg ataactcata
    21061 aatcctcctt ccctcaaaac aacaaaataa cttgagtagt aaattgatac ggatcgtttt
    21121 gatagttcac aaacatattg ggattctttt tattgatttc ctctatacta gataaaccta
    21181 acccttgatg tccctttttt gttgttattc cttttttcat tagagtatct atagagagta
    21241 gtatctctct acaactattc gatatcgaaa tttctatctg tttatcatct cggtaaatag
    21301 ctagagacag ttctctatct tgactctcta tagatgcttc aattgcatta tcgagaataa
    21361 ttccaagcac cctaatacag tcaaatattg gaacaggaac ttcaataata gtacttggac
    21421 attcgaattt acaacaaatc ttattgttat ttgcttggta tagcttcgct atcaacaaac
    21481 ttttcaaata ttcatttttt atatttttta aataacgata ttcaaactta ttggttagat
    21541 aagaattaga atagatctct aattctttta tttctttttc caatatcgaa tcatggtttt
    21601 gattagcgat ttcttttaaa ctcagcaata aatttttata gtcatgtcta aatctggcaa
    21661 atttttcttg atctttttcc agtgaatcag tatatttttt tagataaacc aattcttttc
    21721 ttttcaattg ctgttcatat tgttctcgtt gcctaatagt tattctaata aataaaaata
    21781 gaacaacaaa catttgaatg attaaaaata ttaagacacc aaacacaaaa tgatcaaata
    21841 cttgatattg atgagcaaca taagaaacaa ataaaccaat tgtaagtaaa taaatcatga
    21901 atatagcagt aagtttagaa ctggactgtt caacaatcct atttatagct aattttctat
    21961 ataaccaaat aaacatcagc aaaaacaaag aatcaagcat tatctggaat actacataac
    22021 catacccttt tatcccttca aatgaaaaca tatacgtcat aaccatggaa gtaataatag
    22081 atctgaaatc atcaataagc atacataata gtaatttatt taaaagcact atattagttt
    22141 tcttcttttc ctttaatata aaataacttc cgattatagg tatagctgcg gaaacatcag
    22201 taaataattc aacaaaaaaa gtgagtggta tcaataaaca tacaaaaaga tattttcttt
    22261 tgttaagatc agtattagtc atataaaata agagaaaaaa gaaaaagttt cccacgaaca
    22321 ttcttaatag ataagctttt gtatcgatag taatcaagag atcccctcct gattttagga
    22381 tatcataaaa aaaattttag ttacactttt taaataatga aaaaacacat tccactaaat
    22441 ttgatgccgg ttttccttga ggttttgttc cagcacctcc tttaattttt ttaagctctt
    22501 gatctgaaca ttgtttagca tttagtctat ttttttcttc cattacgatt ccccctacaa
    22561 actataaata cttttatgtc actgattcaa tatacctatt tttctaatca aaaaattttg
    22621 agacatattt aatattataa gtatcattaa aattgagatt tatctccata atctgctcga
    22681 tataaaccat ttattccagc agttcttcca atttcactca aatttcttat taattcctct
    22741 tgatattcag tgaatcttat tttattagta taagcagtaa aatatagata attcaccaat
    22801 ctatttatta aaggaccggg atctaattca tatttttgtt tttctaattt taaatatacc
    22861 ttttgtaaaa cctcaagaat atcagaatat ttaggatctt tacttataga tatatcatta
    22921 tataattcat gaacaatttg cttagcattt tttttcattt tagcacttcc ctggaattgc
    22981 tccacctaaa aaaccaccta tagacattcc tgcaatacaa gtagttgcct tggcccaatc
    23041 gaccgtacat ttatttttag tgcaatacac tccatttcca taatattttc cactatgagt
    23101 ggtaccccca tagataagtt gtgtctcttt aatagacaaa atttttaaat gtttcataat
    23161 ctaatcaccc ctaaattaat gtttttagac tattattcta acattcatta aatttcaaaa
    23221 aacaattcct tcttgaaacg tagcaaaata attcctgaat aagaatttaa tcacttttaa
    23281 gtatataaac tatgtatatt ctttaatatt ttttgagaat gcacttcgta cataataaac
    23341 tttaatcaca ttagtctttc atacctacga tcgatctctt tgactcgcac ccataaattc
    23401 ataatagttt gtcggcgtct ttggacgttt cgaagatgag ctacgcattc tcttttttta
    23461 tagacaaaaa agatagagac gaacatggag cttccaaatt tggatatttg atccaatttt
    23521 ggaagctcca tttatttcgt gtctgtcttt ttattttttc cagctttcga ctaattcttg
    23581 gttctctttg atccagtctt ttgctgcttg ctgtggatct tttccattgt tgatttccaa
    23641 cataactttt tccatatctt tttctgacca atggaaattg tctaatactt tgtaagcttc
    23701 tggctggtct tctttcaatc cttttcgaac catcgtatgg atctgctctt cgcttcccat
    23761 tccgttttca ggatcttcta agtatttcag atcatatttg gcaaacatcc aatgaggtgt
    23821 ccatcctgta actacaattg gttcgtgttt tttgatcgct tgacctagtg caacggtcat
    23881 tgctcctgaa gaagatgtct ctactttcca gtctttcaag ttgtcatatt tttggatcgt
    23941 gttttcagca gccgtaacta caccagctcc tggctcgatt cctatgattt tcttacctgc
    24001 ctgatcggtt aactcatcga tcgaatttac atccatgtag gctggcactg ctaaaccaac
    24061 ttttgctcct gtcaaattgg cacctaggtc ttcgacttga tctttatatt gtgcatattg
    24121 tgaaccatga gttttaggta gccaagctga aaccatggca tctgactcgc catttgaaac
    24181 agatttccac atgattgaat tatctaatgg cgtcatcgtt acatcatagc ccatttgttt
    24241 caatacttca ccgactacat ttgttgaagc aacttctgta tcccattcaa cataagataa
    24301 gttgatacgt tttgtttctt tggcagatga aaaactccct ataccaatcg cacctaagat
    24361 cactactact acagcaccaa tgatcagtcc ttgacgtttt ttacttttct gtgttgttgc
    24421 tcctgcagct tttttattgt ttggctggtt caaatgctgt gtaaaacggt caatgatgat
    24481 tgctaagata actaatgcaa ccccgttgac aaatccattc ccgacttgtg ctctttgcaa
    24541 tgctgataat acgccgcgtc ctaagccggg tgcaccgatc atagaggcaa taacgaccat
    24601 tgaaagtgac agcattgttg tttgattgat ccctgccatg atcgtacttt tagctaaagg
    24661 caattctagt ttgaatagtt tttgacgact tgtgctcccg aatgaatcag aagcttctac
    24721 taattcttta ggaacttgtc gaatccctaa attcgtaaaa cgaacggttg gtggcagagc
    24781 aaagatgact gaggcaaata cccctggaac catcccgata ccgaaaaatg caactgctgg
    24841 aatcaaataa acgaatcctg gcattgtctg cataaagtca aggatcggtt tgataatact
    24901 ttgggcttta ctgctttttg ccatcaagat ccctaatggt accccaatga tgatcgagat
    24961 cacactagat aaaagaacaa gtgtgacagt attcatcaaa tcattccata gaccttggtt
    25021 ataaataaac catagaccga ttaatgtgaa caacgtaagt cctggacgtt tcttagaaat
    25081 aaagaaagct gctaccgtca ataacaagat aaacaaagga gctggaatca ctaacagtaa
    25141 atttgtaatc cccgacatca ctgcctgtcc aatcgtttgt aaaaatgaaa ataggccaga
    25201 aaaagttgtc gtaaaccatt ctgtgatatt ttctacccag tttgccactg gtaattgata
    25261 gttcaataga ttattcatgt tcactcacct cgcttgtttc tgctaatgct tcaatcacac
    25321 tacctcggat gacgacacct tttaattttc catcctctac aactgcaagt ggagtcggtg
    25381 agtcataaat aatattgaaa atatcactta ccaccatgtc tttcccaatc accgtcacat
    25441 tttcatctag gaagtcgatc aatggacgtt tttgcttcct agcttctaac gcttgatctg
    25501 cagtgatgat ccctttcagg tgtctctttc tgtcaacagc catcagcatg ctgacttctt
    25561 cattacgcat ccttgtcaat gcaacgtttg gtccatcaga ttcgatattc gtcgtcaatg
    25621 caggtaccat gatgttttga gccgtaagca ctttggaacg atcgacttct tccacaaatt
    25681 cacgtacata atcatttgct ggattggtca atatttcttc acctgttccg atctgcatga
    25741 tttcgccgtc tttcatcaag gcgattctgt ccccaatccg taaagcttca ttcaaatcat
    25801 gggtaatgaa aataatcgtt ttttgaacat tggcttgcag gtccagtaat tcgtcttgca
    25861 tttctcgccg gatcagcgga tcaagagccg agaacgcttc gtccatcagc aagatctcag
    25921 gatcatttgc taaagctctg gctaggccaa cccgttgttg catcccacca gataactgac
    25981 taggatattg gtctttaaat gaaagcaaac tagaattttc taatgctttt tctgcttttt
    26041 cttgtcgttc ttctttaggt acaccacgga cttctaaccc gtattctgta ttttccaaga
    26101 tcgttcgatg tggaaacaga ccgaagtttt ggaaaaccat attgatttta tgacgacgga
    26161 cttctcgcaa ttcttctttg cttaacttcg atacatcttc tccatcaata tagatactgc
    26221 ccgaagttgg ctcaatcaga cgattcaata gacggatcaa tgtggatttc ccactcccgg
    26281 aaagccccat gatcacgaag atttctcctt cttttacgtc aaaattcacg tcatacacac
    26341 caaccgttgc accggttttc tttaaaatct ccgttttcgg ctggtgatca ttcatcatat
    26401 ctaaagcggc ttgtgtcttt ttaccaaata tctttgttaa atgttttact tctactttta
    26461 ccaaacaaat tctccttttt tatgtaaatt catttttctt tctgaaaaaa gtgaccacac
    26521 tatgttaaca tcatgtagtc actttgtcaa aaatgaaaga aatttgcttg tccaaactga
    26581 aaaaaacttg gctaaatctt tgtaaataaa aggtttagcc aagttaatat ttttttaata
    26641 tttcacacaa tcattctgca agctgatgct ccgggtaaaa aaagtttgtc atacgctgta
    26701 gtgtatattc atttccaata aaatgaacga tgtcccctgc cgtaaattta gcataggggc
    26761 caggtgaaac aagcagttct tcattatgag aaattgcgat aactgtggcg ccagtcgcct
    26821 gccagatatt caactcgcta attgaacggt ccagatgcat tgctttttct gataaagata
    26881 attcaaacgg aagtaaagga ttcatcttat gtattcgctt cgtttgattg accagctgat
    26941 ccaataattg agaaaaatga ttcagctcat cttgttgctt ctccacactt gccatgattt
    27001 ctgcttttgt atcttggatg gattgtacat cttgatattg caataggaaa tcttttgctt
    27061 tttccttcga agcaacaaat gccccacttc catgccgtac ttccatgatt ccaagatcta
    27121 ccagcacatt gatagccttg cgggtagtct caggagaagc attgtacgta ctggcaagtg
    27181 ttgagcgggc atggatcttc tcacccactt tatatcggtt ttcaacgata cgttcggcta
    27241 aatcaacagc aatctgctgg tagcgcgggc ggacgatttt tttctttcca gacattttct
    27301 ttcctctttc tttttttact ttccctcatc ttactatgct ctagcaaaat aagcaaaaaa
    27361 ggggagaata gcctcttgca acgactgatc atcgcttgat ctgttccttt tttactgggc
    27421 aagctgtttg acagtcatca caattcttac cggattttaa acgagtatag acaattgcgc
    27481 ctgcacttcc aaagattaac acacttaata agaatgtcgc catcctattc ctctccttct
    27541 aatgacaata tagtataaaa ctggttcttg gattttggct gacggaacaa aacataaacg
    27601 agtcctatca tcagtaaaat agcaagatac gtccctaacg ctacctgtcc accctccaat
    27661 aagtgtccca actgataaat cacaaaacta actaaataag ctaaaccgca ttgatagcct
    27721 atggcgatcc aagtccattt cttggcattc atttcccgat ggatagcccc aattgccgca
    27781 aaacaaggcg cacaaagtaa attgaaggct aacaatgaat aagctgctgc gggagtatat
    27841 gtgtgttgta gcacttgcca gatttctctg ccattttcgg atacttccgc atgaccaaac
    27901 aaaataccaa atgtgccaat aatattttct ttagcgacca atcctgtaat cgctgcgact
    27961 gttccttgcc agtttcccca accaagcggc tgaaaaacaa ctgccaggat tcgcccgaaa
    28021 aaggctaaga tactttggtc ttcagaaaca ggttgagcat aaaaattata agaagagctg
    28081 aaccagatca cgatactaga aacaaagata atcgttcctg cttttttaac aaaagacttg
    28141 ctgcggtcat aggtttgtct taggaccgct gaccattgag gaaaatgata tgcgggcaat
    28201 tccatgataa aaggagctgt atctccagca aacaaacgtg tttttttcaa gctgattcca
    28261 gagaaaataa ttgcagtcat accaagaaaa taggcagaag gagctaccca agattgatga
    28321 ggaaaaaagg cccctgctac taaggcaata atcggcaact ttgcagaaca aggcatgaag
    28381 gttgtgacca tgatcgtcaa acgacgatct tgctcgtttt caatcgtgcg actggccatg
    28441 acgcctggca ctccacatcc tgtagcaatc agcataggaa tgaaggattt tcctgaaaga
    28501 ccgaatttcc tgaaaagtcg atccatgaca aaagcgatcc tcgccatata tccgcagtct
    28561 tccagaaagc tcaagcataa aaacaatata atcaattgcg gtagaaatcc taacactgct
    28621 cccacgcctg cgatgatgcc gttcaagatc agctcctgca tccatcccgc tacttgccat
    28681 tcagctaaca atctgccaac atgttctggt aaccatgtac caaacaatgt gtcattgatc
    28741 caatctgtcc ccattgttcc tactgtttga atagccagat aatagatcag ccacatcaca
    28801 aatgcgaaga tcggcaaggc taaccaacga ttcgttgcaa tctgatcgat tttgtcactc
    28861 atcgataatt ggaaagaatc atttgacaca acacagagtg tacatagttg ggtaataaat
    28921 tcataacgtt cattgaccag aatcgtctca ctatcgtcgt ccaatagttt ctctgttaac
    28981 tggatcagtt gctcgatctc tttttgctga gaaactgcta tctcgtaatc ctctcttacc
    29041 ttctgatctc gttcaaaata tttcattgca taccagcgtt tcttgccgct ttgttctcct
    29101 agtacatctt caatttcatg taaagctgct tctaaacgtg gatcatatgt acagggaaca
    29161 ggaatagaag gccgcttttt tgcagactcc tgaatctttt cgattagttt ttcgattcct
    29221 tgctttttca atacgctgat tggtaaaact ggtacaccaa gaccatagga aagcttttct
    29281 agattgattt tttttccttc ttttttgatg acatccatca tattcagacc aatgatcata
    29341 ggaatgtcta aatccaataa ctgcgtagtt aaataaagat ttcgttctaa attggtagca
    29401 tcaataatgt tgatgatcag ttcaggctgt tcctgaacca aataatctct tgcaatcact
    29461 tcttctggtg tataaggaga caaagaatag atgccgggta ggtcctgaat atgaatgttc
    29521 ggattttttt tcaacgatcc ttcttttttt tctactgtta cgcccggcca attccctaca
    29581 tattgattcg aaccagtcaa gagattgaac aagctagttt tcccgctgtt tggatttccg
    29641 actaacgcga tattcattct ttttcctccc tttgtatcaa gattagttcg gcctctgact
    29701 ttcgtaacgt cagttcatac cctcgcactg tcagttcgat tggatctccc agaggggcta
    29761 gttttcggat aactattttt acatttcgcg tcaatcccat atccattaac cgtcgcttca
    29821 ctgcgccttg tccgtggatt gctacgacga tcccagtctc tcctgctttt aattgatcaa
    29881 gagaagtcca gctttcagag gaagcagtct ttttattgac aaagatttga cctagtattt
    29941 cccgacttac tgccactcga tgacttttta ccagtaaaat ggcattgttg cctgataaat
    30001 tgacgacgac gattttactt ccggcaacga tcccgatatt ttgcaaatgg cgttttgttt
    30061 tttcttctgt ttcgattcgt tcgacttgat aaacgaaatt ctgctttgcc tgcgctaagg
    30121 tctgcatatt gattcctctc ttgtgaaata aatcatgtat taaaatcatt cattatatgt
    30181 tctaattttc ttacgtgtta cgcaaataaa tgagaatcat tctcacatag aatactctcg
    30241 gtaattgaga atgtcaatta tacttgtgat attatgagcg aatctttagt attgatagat
    30301 aaaaatgaga aaaaaatatt tcttcatagt tttccacaat tttttctcta ttaaatttgc
    30361 tattttccca cacgatactt gttttcagct cttttttcaa aaacacaaaa cttagtattc
    30421 cacagtttcc acaacacaat atgtagtgtt tgttgtttga cttttttact aagagtttta
    30481 tactatctat tatagataat aagaatgtgt tagaggaggc actcaccatg aacgtaaaaa
    30541 tcttttctaa aaataattgt atgcaatgta agatggtcaa acggttttta acagaaaatc
    30601 aaattgcttt tgaagaagtc aatatcgatg aacagcctga agcagtagac tggttgaaag
    30661 cacaaggatt ccaaagtgtt cctgtcatta cttctgatgc agaaacagtt atcggattcc
    30721 gtcctgatca gttgcgtaaa cttgccagct aacatgacta aaagaatcaa aacgtgatac
    30781 atggatttga agggcttgag acagcaccag tttttgtcgc aaacttccaa atcctttttt
    30841 aaagcttaaa aacaatgcaa tgatttgcaa tcaagcatca tcaaatacga aatgaaggtt
    30901 gggcgtacgt atgagtctta aaaatttaca agatgtcagt tatttcaagc tgaataatga
    30961 aatcaatcgg ccagtcaatg gtcaaatccc tttgaataag gataaagaag ctttagttgc
    31021 cttttttgag gaaaatgtca aaccaaatac gatgactttt ctatctatta tggaaaaaat
    31081 caattacttg atcgatcaag aatatataga agcagaattc attcaattgt attcaccatc
    31141 atttattgaa aagctatatc aatttttgga cgagcaacat ttttctttca aatcttttat
    31201 ggctgcttac aaattttatt ctcaatatgc gttgaaaacc aacgacggtt ccgcttatct
    31261 ggaaacttat gaagatcgag tagccttcaa tgcgctttac tttgcgaacg gggatgaaga
    31321 attagcactt gctcttgcag acgaaatgat ccatcaacga tatcagccag ctacaccttc
    31381 tttcttgaat gctggacgta aacgtcgggg cgaattggtg tcttgtttct tgactcaagt
    31441 cactgacgat atgaactcta tcgggcgctc aatcaactct gctttgcaat tgtcccgtat
    31501 tggtggcggc gtagggatta ctttatcaaa tcttcgtgaa gccggcgctc ctatcaaagg
    31561 atatgagggt gctgcaagtg gtgtcgttcc agtcatgaaa ttattcgaag atagtttcag
    31621 ctattctaat caattaggtc agcgtcaagg agctggcgtg gtttatttga acgttttcca
    31681 tccagacatt attgctttcc tttcagcaaa aaaagaaaat gcagatgaaa agattcgcgt
    31741 aaaaactctt tctcttggcg tgatcgtacc tgataaattt tatgaattga cacgtaaaaa
    31801 cgaagatatg tatttattta gtccatatag tgtcgaaaaa gaatatggtg tcccattctc
    31861 ttatgtaaat attacagctg agtatgatca tctagttgca aatccaaaca ttcgcaaaca
    31921 aaaaatcaaa gcgcgtgatc ttgaaaatga aatctcaaaa ttacaacaag aatctggcta
    31981 tccttacgta atcaatattg atacagcgaa ccgcgagaat ccaatcgatg gcaagatcat
    32041 catgagtaat ttatgttcag agattttaca agttcaaacg ccttctgtga ttaacggcaa
    32101 acaggaatat gaagtattag gaacggatat ctcatgtaac ttaggctcga caaatatcgt
    32161 gaatttgatg caaagcccta actttggtca atctgtgcgt acgatgactc gtgcattgac
    32221 atttattaca gatgcatctg atatcgatgt cgtaccaccg atccaaaacg gaaataagct
    32281 gaatcatacg attggattag gtgccatggg cttacacact tatcttgcaa aagaacaaat
    32341 ggaatatggc tcagaggatt cattagactt tacaaatatt tacttcatgc tgttgaatta
    32401 ttggacactt gttgaaagca acaatatcgc tcgtgaaaga aaacaagttt tccataattt
    32461 tgaaaaatca gcttatgctg acggcactta tttcgaaaaa tacgtaactg gagaattcca
    32521 gccaaaatca gaaaaagtca aagcattgtt tgaaggcatt tttatcccaa ctgctgaaga
    32581 ttggaatgct ttgaaacaag cagtaatgaa agatggactg tatcatcaaa atcgcttggc
    32641 agtagcacca aatggctcaa tttcatatat caacgatacg agtgcaagta ttcatcctat
    32701 cactcgcatg atcgaagaac gccaagagaa gaaaatcggt aaaatttatt acccagcgcc
    32761 ttatctatcc aatgagacga ttccgtatta cacttctgct tatgatatgg acatgcgtaa
    32821 agtaatcgat gtttatgcaa tggcacaaca acacgtcgat caaggcatga gtttgacttt
    32881 attcatgcgt tctgaaatcc cagaaggcat ttatgaatgg aagacaacaa cgaagcaaac
    32941 gacacgtgat ttgaatattt taagacatta cgctttccac aaaggaatca aatccattta
    33001 ttacgtacgt acctttactg atgatgcaga agaaatcgga agcaatcaat gtgaaagctg
    33061 cgtgatctaa tatcatagat agattcgctt attcatattt taattaaagg agcattctca
    33121 tgtcagaaac atattatgaa gcaataaact ggaatgaaat tgaagatatc attgataaat
    33181 ccacttggga gaagctgaca gagcaatttt ggctagatac acgtatccca ctttcaaatg
    33241 acctagatga ttggcgtaca ttgtctcaat tggaaaaaga tacagtagga catgtatttg
    33301 gcggtttgac actgctagat actgtccaat ctgaaagtgg catggatcag ttgcgtaaag
    33361 atgtccgcac tccccatgaa gaagccgttt tgaacaatat tcaattcatg gaatccgttc
    33421 atgccaaaag ttattcttct atttttagta cactgaatac gaaaaaagaa atcgaagaaa
    33481 tttttgactg gacaaatacg aatccttatc tgcaaaaaaa agctgagcgt atcaatgaaa
    33541 tctacaaaca cggtacaccg ctagaaaaga aaattgccag cgtcttttta gaaacattct
    33601 tgttctattc aggtttttat actcctcttt attatttagg aaacaacaaa ttggctaacg
    33661 tagcagagat catcaaacta atcatccgag atgaatctgt acatggaacc tacatcggtt
    33721 acaaattcca attaggattc aacgagttac ctgaagcaga acaagaaacg ttaaaagact
    33781 ggatgtacaa tcttctttat gagctttacg aaaacgaaga acgttataca gaagaattat
    33841 acgatccaat cggctggaca gaagaagtca agacattcct acgctataat gccaacaaag
    33901 cattgatgaa cctagggatg gatccactct ttccagatac tgccaatgat gtcaacccaa
    33961 tcgtaatgaa cgggatttca acaggcacaa gcaaccatga cttcttctca caagtcggaa
    34021 acggctattt gttaggtcat gtagaagcga tgaaagacga cgattatttg attggattgg
    34081 aataaggatt tattttaatt caaaaatttt tcgaaaaata aaaagagttt agcgtacaga
    34141 tattttaaat gtacactgaa ctctttttta tgcttattca cttgtttctt ccagcacttt
    34201 tggactgtct aacttttccg aaacatgttc cattcttctc aataaataga ttttccggat
    34261 atttaatatt atcgtcttaa tcactgcata gacaggtatg caaatcagca tgcctaatat
    34321 tcctgcgaca cttcctgctc caatcaatac caagatgatc gtcaaaggat ggatattcat
    34381 tgatttccca aataacaacg gcttgatcaa atttccatct aattgttgga tcactaaaat
    34441 cgacaacgcc atatacaaag cttgaaatgg agagataaac aaaccgacaa tgattgctgg
    34501 taccgctcca ataaaagggc cgacatacgg gataatattc gtgattccgc aaatgaccgc
    34561 aagcaacaag ctataaggct gatcgaatat tctcattgcc aaaaagctca ttacaccaat
    34621 aatcaaagca tctaagatcg tactgctgat ataggcagat aatgtttcgt ttaattcttt
    34681 taccgtttgg cgtaactctt tacgaatcgc tttcggaaag aagtgagaca acgcatcaat
    34741 aaatttgtgc ccatccttga acataaatag caaaataaat ggaacggtaa ataacaaaat
    34801 aaaaaattgc atcataaatg aaatgatttt tgagacactt gcagataaac tgactaatat
    34861 gatattgaac aaattactaa gagataaatt tgctgatgct aattgttctt ccaaattgaa
    34921 atttttaaac tcttcccgct gcgccaattc atttaaccat ttgcttgttt catctgcata
    34981 cattggcaag ctctgggtca attgaattgt ctgttccatt aactgaggca ctacattcat
    35041 gacagttaaa acaactaaaa tgattacgac caaaaacgtc aataaaaaac cgaacactct
    35101 cgggatccga tgcttttcga gaaaaacaac gattggatcg aacatataat agataaaacc
    35161 agcaacaagc aatggtaaaa aaactgccga aagcattttc aatattggac taaaaatatt
    35221 cggcatttgt aaaataaagt acacgccgat gatcgtgagt actagctcca ctgtccagaa
    35281 aaataatttt gattgtttaa aacgtgattg catacatctc ctccaaggtc ctttgatact
    35341 tctattctat catttaacaa agagttacgc gctgagattt tcatttgctt aaaaaaaaca
    35401 aaaaaaataa acatatttta atgagtagac gagtacttaa acaaaaacac ggtttggagt
    35461 agtgacaaaa attatactgc agtccctttt tccaaataaa cggtgttcaa aagcttgcat
    35521 cttcgtaata agcccagcca aacgaaaaat ataaggaact atttttattt ggctgggctt
    35581 attacttgat gtaggacatc tattttacaa cctcagatca tttattgcgc aatactgact
    35641 aaatgcaact ttgtgatatt tttgtgtacg gacaactcct cgatcacatc ggcataagtg
    35701 agcccttttg gaagatcaat cgtatagata tttttgtaca ctcggtagtc atcaacgaat
    35761 tttacgtcaa aattcacatc ttcgattacg atatttttct cttcaaaata atcggtaaga
    35821 aaatctttgg tttcttttct atggataaat tggatttcta actttttagt cgtaggtaca
    35881 tggatcacgc gtttcactac tgtgagtgca atagcaatcc cgataaaact gatgatcgca
    35941 atggaatagt aacccatgcc gatggcgatc cctaatccag caattgccca taaagaagcg
    36001 gcagtcgtaa gtcccgtcac cgattgtttc gtgacgatga tcgttcccgc gcctaagaaa
    36061 ccgacaccac tcaccacttg agcaatcagc cttgcttcat ctgaacgaat cgttccagca
    36121 agctgtggat actctaaagc atctcttaat gcaccactag cgatctcaac ttggatcaat
    36181 gcaatgatac atgctcccat acaaactaaa atatgcgtac gcatacctgc tggacgattt
    36241 ttatattgtc gttcaaaacc gattgctccg ccaaatagca tagcgatccc taaacgtaaa
    36301 ataatctctg gaattgtcaa taccatatca ttcatctaaa caccctccag tccaatatat
    36361 catgtctgaa aggagcagac aaaaaaagtg caatttttac ctcttacgaa aaaaggactg
    36421 tgacaaaatt tttgtcatag tcctttttat gaataaacgg tgttcaatca gctgaccctc
    36481 ggtattactc aacaaacggc aaaatatggg gagctatttt tcctttgtct tctctaatac
    36541 tcaggtcaaa acgctgattt cacaacctct ttagcctact attgaaaatt acaacttact
    36601 tgctgcttct tcatcgatga tcacaacaac attgtcgtga ttttgcaata cacttgctgg
    36661 caaatgattc gttacaggtc cttcaatcat ccctttgatt gcatcagctt tgctttcgcc
    36721 aaatgccatc aagacgattt ctttactttt cataattgaa ccgattccca ttgaaacagc
    36781 ttttgttggt acatcttctg ctttatcgaa gaatcgtttg tttgcattga ttgttgattc
    36841 tgttaagtca actactgaag tcgttccgtc aaatgaagca ccaggttcat tgaaaccgat
    36901 atgtccattt tgcccgattc ctaagatttg gatatcgatt ggatgtgcat cgatgatttg
    36961 atcatacttt ttgcatgctg cttccagatc ttctgctttt ccatctggta caaacgtttc
    37021 tttgaatggt tttttatcaa ataattggac atccatgaaa tgacggtagc tttgttcatc
    37081 tgaaccgcct agtccaacgt actcatctaa gtttacagaa gtcatttcac tgaaatccaa
    37141 atcgcttgaa gtcatttctt tatataaagt aactggtgtg cttcctgttg ccaatcctaa
    37201 tacttttgca cctttgttca taccttcttt gatgatttca aatgcttttt ttccgccttc
    37261 ttcagcgttt tttacacgaa tgatttccat agtgattgct cctttatatt ttggtatagc
    37321 ccaattatag cattcttttt ttctttttac aataattagt atagaccaaa ttgcattttt
    37381 ttcattttct tgacaataaa taggaatccg tgttaggttt cataagatgg ttcaatcaaa
    37441 caatccatgt tgcatgaaaa ccaagcaagt gattcactgt ggacgcttta tgtctgcagt
    37501 ttttttatga aaaaggagtt attatgagta tactgacatt aaacaacatt acccagcaat
    37561 ttggcgacaa aatcctctat gaaggtgtcg atctgcaatt aaatgctggc gaacatcttg
    37621 ggctgattgg acaaaacgga gccggtaaaa gtacattgat taaaatcatc actggtgaga
    37681 tcttaccaga tgacggacag atccattggc aaaaaaatat tcacaaaggt tacctcgatc
    37741 aatatgtgga agtcgatgaa acattaacaa tcgaagaatt tctaaaaact gcgtacgcaa
    37801 aagaatacga aaaggaagaa aaaattgctt ttctttacca aacatatagc gagaccttcg
    37861 atgattcctt gctggaaaaa gctgggaaac tccaaacgga attagaccaa ggagtgtttt
    37921 atcaaatcga caccttagtt gctgagatga gcagcgggtt ggggatcgat gtcttaggtt
    37981 tagattcact gttgaaaaat ctaagtggtg gacaacgttc aaaggtgatt ttagcaaaac
    38041 tacttttaga aaacccagat gttctgatct tagatgaacc aaccaatcac ttagatgatc
    38101 agcatatcca atggctgacg cagtttcttc aggattttga cggtgcgtat atcgtgattt
    38161 cccatgacca agaatttctt gatcagatca ccacccatat cgcagacatc gagtttggaa
    38221 agattactaa gtatactggc catctaaaac aagcattgaa acaaaaggaa caaaatcgcg
    38281 aaagctattt gcgccaatat catgctcaac aaaaacaaat cgaaaagacc gaagcttata
    38341 ttcgaaaata caaagccggt tcccgatcaa cgatggcaaa aagccgacaa aaacaattag
    38401 ataaaatcga acggctgacg cctccagctt cagcaagtaa agctgtcttt gattttccat
    38461 tctcgcctat tgtcacgaca ttagcagtgg aaacaactga tttagtcatc ggatatgatc
    38521 gaccgctact tgcacccatc aatttgacca tccgctttgg tgaaaatatt gccattcgag
    38581 gcttcaacgg gattgggaaa tctactttat taaaaacact gatcggtgag attgaaaaaa
    38641 taagtggcga tttccacttc ccagataaca caaaaatcaa ttacttttca caagatctgt
    38701 actgggaaaa tccattagaa acaccactac aatatttaag cagtcaattt ccaaaagcga
    38761 ctatcaaaga attacgtcgc caactagcta aagcagggtt agttaatcaa ttagcaagtg
    38821 aacctctcac tacattaagt ggtggggaac aaactaaggt gaaacttgca cagttgacaa
    38881 tgaaccaagg taatttactg attctcgatg aaccgacgaa ccatatcgat caagaaacga
    38941 aagaaagctt acaagacggg atacagaaat ttccaggaac agtaatcatc gtctctcatg
    39001 aacaagaatt ttatcaagat cttgtgcaac gagtcattga gattgaacca aaataaacgt
    39061 ttgattgaat agttgaaaaa aaggagctgt gacaaaagtt ttgtcacaac tccttcttct
    39121 gaataaatgg tggccaatta gctgaccttc ggtattagct caaaaaacgg caaaatatga
    39181 ggagctattt ttatttggct attcttatta cttgatgcag aacagctttt cacaacctct
    39241 tttcgtctat tttttatttt cgagatttct ctttccttta aaaacaactg tttgaaagaa
    39301 gaacgcacat ccttgaaaaa caagcccgct aaccgttaca cctaaccagc catactgctg
    39361 ccacattgtc gtccctagca atgaaccagc cgcacctcca ataaagtaca taaacataaa
    39421 tacagtatta tttcggttgc ttgctgcttc acctagccct tgtactcgtg tttgattggc
    39481 gacttgacca aattgattcc cgacgtctaa taaaataata gctaaaagca aaatggccaa
    39541 atggcttccg ccaaccaata aagcaataaa gcttattgtt tgcataacaa gtcccatata
    39601 cacgattttt ctttcagaat aagaatctgc cagtcgtccc acgataggtg cagcaactgc
    39661 tccagacaaa ccaaaaatcg ctaatatccc tgcttcgaat gttccccaat tgtaggcggg
    39721 actgctgata taaaaaatca acgtagacca aaagatcgaa aatgttccaa acatgaaaaa
    39781 tccactgact gccgcttctc ttaatagacg ttgagaagca actaaatgcg gcaaactttt
    39841 aagtgaatca aggtatgaaa cagctgtact actttgctgg acagaacgcg gcattttcca
    39901 gtaaagcaga agtgtaagaa ttccgactgc acataaagca aataaataga tcatccgcca
    39961 agaaaaaaga ctggcaacaa taccagatac cgtccgagaa agcaaaatcc ccacgagtaa
    40021 accgctaagc aatgtaccca tgaccttccc tcttgctttt ggtcccgcta gtaccgcacc
    40081 gtaaggaatg atgatttgcg gcacgatcga aagtaatcct attaaaaaag aagcgcatgc
    40141 aaataacgta aacgaaggcg caaaaaaagc ggccaacaga gagacggcag ataacgctgc
    40201 cattcgaatg atcaattttg gacgatccac cacgtcccct aaaggaacta aaaacagtaa
    40261 ccctaacgca tagcctaatt gggtcaacat cgtcaatatc ccgactgcac ttgtgctaac
    40321 agataaacta gctgctactt ttgttccaat cggctggatg taatacatat tggcaacaac
    40381 aacgccacaa gtgattgata atagtaaaat aagcgaagtt gttaaaggtt ttttttcatt
    40441 actcttcatt gatttccccc atttcctatc ttagtttaca acttatggaa aagattggaa
    40501 atagaaaaat acaactattc gccacactta ttatttgtgc ttttgttttc cttgtttttt
    40561 cttgcgatct ttataaatga agacaactaa ccctactagc acagtaacac ttgcaaccaa
    40621 cgtgactgca gttttgttat atccgtgaaa cagagcttct ccaccacacg catacaacac
    40681 agacgaaggg atgactccaa tcacgatcga caatatgatt tgctgtagag gcaatttcat
    40741 cgcatctgca gcaaaattga ccatagaaga tggaataacc ggtatcatat agcctaacat
    40801 tacacctatt tttggatgtt tcatttgtcg gattgcttgc acccaatgat tcgtttcggt
    40861 tttcttatct aaaaatttta agcgatgcat aaggaaaatt gcagtgatgt taccaagagc
    40921 attacccaaa atgttgatga tcgcacctgt cagtggtcca aagctcaaac cagcaatgac
    40981 tccaactact gaagtaggaa tacctggaac agcacacatc accgctgtca atacgatcaa
    41041 gagtatcgat gcaaaagtcc catgcgaacg aatatgtgcg agtagctctg ccttactcgc
    41101 tctcggattt aaaaataatt ggatgtctgt ctgatattct aaatacaacc gatagcccac
    41161 taccagtatc agaatgattc ccaacgagat caatatcaat cggattattt tctttttcat
    41221 atttcctcct gaaaaaaatc tatattcatt aaataatagt ttaacataaa aacaaaaaag
    41281 caccgaaatc caacatagga tctcagtgct tttaaatagt aaaaattatt taacagttac
    41341 agatgcgcca acttcttcaa gtttagcttt caattcttca gcttcttctt tagatacgcc
    41401 ttctttaact ggtgcaggag cgccatcaac tacagcttta gcttctttca agcctaaacc
    41461 agttgcttca cgaactgctt tgataacttt aactttttgg tctccagctg cagttaattc
    41521 tacagtgaat tctgtttgtt cttcagcagc agcagcgcca ccagcagcag ctacagctac
    41581 aggagcagca gcagatacgc caaattcttc ttcaatagct ttaactaggt cgttaagttc
    41641 taggatagtt gcttctttta actcagcaac gatgttttca atgttcaatg ccattataat
    41701 ttcctccgtt taattgttct tttttattta atgctttatg cggcaattaa gctgcatctc
    41761 catcttcttt gtctgccact gctttgacag cgtaagccac gttgcggact ggcgcttgta
    41821 gtacagatag caacatagat agaagtcctt cgcgatttgg caattttgcc aatgctgtga
    41881 tttcttccaa tgaagagact ttaccttcga tgataccgcc tttgatttct aatgcttttg
    41941 cttctttagc aaattcgtcc atgattttcg ctggtgcgac tacatcttcg ttactgaatg
    42001 caacagctgt aggtccagtg aatacttcgt ccatgccttc aaggccagct tttttagctg
    42061 cacgtgaaag aatagaattt ttgataactt tcatttctac gttagcatca cgaagttgtt
    42121 tacgtaaacg tgttacttca tcaactgtta atccgcggta gtcaacgact actactgatg
    42181 cagctgctgt aaatttttcg cttacttcat caacgattgc tgcttttttt gcgataattg
    42241 cttcactcat ttttgagttt cacctcctga attttgatgg aagagttttt tgcactgaaa
    42301 aggcaagtca cttttatagt tactttgctc aaccagtatt attcatcacc tattcacttc
    42361 gttcatagtg attctactcg ctttgctcga aaggaaactc ctttcgcttt gctcaaccag
    42421 tattgttcat catctcttca cttcgttcat agtgattcac aataaaaaac tctatgccac
    42481 cgcagacata gagggactat tgaatatatt caataaacgt cctcggtagg aaattaaggc
    42541 ttgatgccac ctactgtctt cggtacaagt taattattaa cctcaactag cttagcatgc
    42601 taaactagtt gagtcaagaa ttatttttca gttggatcaa tcaacattct aatatttaat
    42661 tagaaagaag cttggtcaac atggatacca ggtccaaatg ttgttgtgat cgttacattt
    42721 ttcatgtatt gacctttagc tgctgatggt ttagctttta gcaatacatc atggattgct
    42781 gcaaagtttt caactaattt ttcatcgtca aatgaaactt ttccgattgg tacatggata
    42841 tttccagctt tgtccacacg gtaagttact ttaccagctt tcacttcgtt tactgctttt
    42901 gttacgtcca ttgtaactgt accagtttta gggtttggca ttaagccttt tggtcctaat
    42961 actcgtccta gacgacctac agtagccatc atatcaggtg ttgcaacaac tacatcaaag
    43021 tcaaaccatc cgttttggat tttttgaacc atgtcgtcgt cacctacgaa atctgcacca
    43081 gctgcttcag cttcttttgc tttgtctcct ttagcaaaaa ctaaaacagt ttgtgtttta
    43141 ccagttccgt ttggtaatac aacagcacca cggatttgtt ggtctgcttt tttagggtct
    43201 acgttcaaac ggtatgcaac ttcaactgtt gcatcaaatt ttgcaatgtt tgtttctttt
    43261 gctaaagcta ctgcttcggc aacaggataa actttacttg aatcaacttt ttttaatgca
    43321 tcttgcattt ttttgctctt tttagccatt ttggttcctc cttgattgtg gttataacgg
    43381 ttgtacctcc cacgtgttct atatctttca atatagagcc aactgagaag ctgctgtctt
    43441 agggtgtata gattattcta cagtgatccc catgcttcgt gcagttcctt caaccatgcg
    43501 cattgctgct tcaacgttag ctgcgtttag gtcttccatt tttaattcag cgatttctct
    43561 tacttgatcg ctagaaactt tcgcaacttt atttttgttt ggttcgcctg aacctttttc
    43621 gattttagct gcttttttca atagaacagc agctggcggt gtttttgtaa cgaatgtgaa
    43681 tgaacggtct tcgtatacag aaataacaac cggaatgatc aaacctgctt gatcagctgt
    43741 acgagcgttg aattcttttg tgaatcccat gatattgata cccgcttgac ctagtgcagg
    43801 acctactggt ggagctggag ttgctttacc tgcaggaatt tgcaatttaa cgattttttc
    43861 tactttcttt gccacgagac atacctcctt aagtccgtga tgtggttaat tggagatgca
    43921 tatttctcct cccacgtttt atctgatctg aaaaaatcca gacaaataat gtgcaaaaca
    43981 atgcacactg gtacagtata gcaaatttat ttgttttttc aagatttttt tctgctattt
    44041 ttacattttg cttgaataaa cgctaaattt attctaaaaa aacaacaatt tttcgttctc
    44101 atttcttctt ttatgttaca cttgcgctag catggttcaa cagatgaaag caaacaataa
    44161 cacggttttt tagacccttg tacaaagatt cctacataac aaaatgaggt ggagaaaatg
    44221 aaagatttaa ggtatagaag tgtctttgat attattggac ctgtgatgat tggtccaagt
    44281 agctctcaca ctgccggagc tgctagaatc gggaaaatca tacgtagtat atttggcgaa
    44341 cagcctgatt ctgtagatat ttatctatat gaatcatttg caaaaaccta tcgggggcac
    44401 ggaacagata ttgcattagt tggcggcctg ttagggatgg aacccgatga tgaacgttta
    44461 gctgattctt tgaaattggc atatgaagcc ggtatggaag tcttgtttgt tcctaagagt
    44521 gaaaaagccg aacatccaaa ctctgtaaag atgcttctaa aaaaaggaac acgaaagctt
    44581 tcagtcactg gcatttcgat cggcggtggc aatatccaga tttctgaatt gaatggattc
    44641 aaaatttctt taagcatggg tactcctacc ttgatcgttg tccaccaaga tgttccaggt
    44701 atgattgcta aagtgactag tatactttct gagaccaata cgaatatcgg cactatgaca
    44761 gtgacacggg aatcaaaagg tgaaaatgcc attatgataa ttgaagtaga tgatcctcaa
    44821 gtagaagaaa cggtaaaaag actcaaacag ctgccaaata tcgacagcgt aaattatttc
    44881 gattaaggag ctaaaaaaat gtttttatcg attgaagaat tagtagatca agcaaaagct
    44941 tatccaagtg tagcagaatt gatgattgca gttgaaatgg aaatgagtgg ccgttcccgt
    45001 agtcacatca tcgagatcat ggaaaaaaac ctctcagtaa tggaacaatc aatcaacgag
    45061 ggaaaagaag gtgttacctc cgtcacagga attaccggtg gtgatgcaaa aaaattagat
    45121 gattatttaa atgaggcaga ctttttaagt ggagaaacaa tcttaaaagc tgtacgcaat
    45181 gctgtagctg ttaatgaagt caatgcaaaa atggggttga tttgtgctac accgactgcc
    45241 ggaagcgcgg gtgttgttcc tggtgtgctg atggctgtcc gagatcggct tgatttgaca
    45301 catgaacagc aattggaatt cttatttacc gctggcgctt tcgggttagt catcgcaaac
    45361 aacgcttcta tcagtggtgc agaaggggga tgtcaagcag aagttggttc tgcaagtgca
    45421 atggcagcag cagctttggt ttgtgcaaaa aatgggactc ctcatcaagc agcacaagca
    45481 gtagccatta cattgaaaaa tatgatggga ctgatttgcg atcccgttgc aggcttagtt
    45541 gaagtcccgt gtgtcaaacg taatgcttta ggctcttctc aagcttttat ctctgctgat
    45601 atggcattag cgggtatcga aagtgtcatt ccgccggatg aagtcgttgc tgcaatgtac
    45661 caagtcggac gtcaaatgcc gtcgattttc aaagaaacag cagaaggtgg cttagctgtt
    45721 acgccaactg ctaaacgact tgcggaagag attttaaata agagggcgtg ataaagctcc
    45781 ttaatttcga gtattaagag attagttatc ttcataacaa aaaacgaaga gaggactaat
    45841 tgcccttctc ttcgtttttt attaatccat caatttcgta ttttttactt catcgatcgt
    45901 ttttgttcct gcaagttgca tcgtgatcga caattctttg ttcaggtgtt caaaaacaga
    45961 tgttactcct tctgccccac ctagattcaa cccataaatc actggtcggc caatcgcaac
    46021 taagtcagca ccacttgcca gagctttgaa gacatgctct cctcgacgga caccagagtc
    46081 gaaaatcact ggtacacgtt tgttgactac ttcggcaatt tttggcaata cttcaaagga
    46141 cgcaggacct ccatctagtt gacgtccacc atgattggat acccagatac catctgcccc
    46201 tgcagagata gcgattactg catcttctgg cgattgtatt ccttttacga taactggaag
    46261 atgggtgatt tctttgattg ttttgatgtc atctggtgtc aagccttgtt ttgcagctgc
    46321 ataaatttct gcgattcctt ttccttcgcc attaccactt gcactttgtt cgctatacgc
    46381 agctaagtta ggcattggta atggaaattg gaattgattg atgacgtctt cttcacggta
    46441 accaccgagc gttgaatctg ccgttaaaat gatagcttta gcccctgctt ctactgcttt
    46501 gttcaaaatg aactcattga atctgtcgtc tttactcata tataattgga aaaactgcgg
    46561 tgcgcctggc gctgcatcgg ctgcgtcttt gattgtcgta tttgcataag tgctgatgga
    46621 aaagattgat ccagcagcag ctactccttt agctgtatca gcttcccctt tttcatgtgc
    46681 caatccttgg gccgctgatg gcgcttggat aatcggtgta tctaattcaa ttccgaaaac
    46741 agatgtatgc aaatcggctg agtcgattcc gcgtaatatc cgtggcatga ttttctttgt
    46801 attaaaagaa gtcgtatttt ctttcatcgt ccactcatct tcagaaccac cgcgaatata
    46861 gccaaaagct cctgcttcca tgcgctcttt tacacgacct tctagttagg caatgtttac
    46921 aatatcgatt ggatgttctt cattacttgt atgataagtc atagtagatc cttctttcat
    46981 cttcttgatt acatataaat catactaaac ttacaaaaag taagcaaaca tttactctca
    47041 gatactttta tcgctattct ttccaaataa atcgacttac aagctgatct acttcttggt
    47101 tttcatgaag catactatga gaggcatttt cacctgtcac tactttttcc tctatttcat
    47161 ttccgcgttg cttcaacaac gcatagaccg ctaatgcact gtttaacggt actgttccat
    47221 cactcaagtc tgtttcatct agttgaccag caatcaagaa aaatcgagtg gttataggaa
    47281 tattaccgat catttgctgg taatcttgat agcggttaga aacgacagca ggcccctttg
    47341 ctaactcatc tgtcagagac tgcgcactat catcgacaaa atcattaaaa ggcgcgccaa
    47401 ttgctgttaa tgtctgaact tgtggcaatg acgtatcctg tccatacgtt cctaaatagc
    47461 gtagtgcact aactcctccc atggaatgcc ccactatgtt gactttatct atgccgtagt
    47521 tttctttcag atacaaaaga caagttttga tccattctgc ttgattccac tcgttatttt
    47581 tattatcttc aaacaatact tggaccatcg gattgtctgc ttttccagac aaatctcctg
    47641 ttgcttgtat ttgtccatct gttcctactg ttaaaaccaa ttcttttact gcttgctgat
    47701 ttttttccaa acgccgcaac atccctttaa aggaattttc tgtcccgctg tagccgtgga
    47761 aaaaaagtgt tggaatagtg gttttttcta tcactttact ggattctgcc gttttttctt
    47821 tatcagaaac ttcagtagta tttatcttcg tcgtctggca accacttaac gcaaacaaac
    47881 tgataaggac taaagctgct gcagttgcac gtttgcataa atttttgatt ggttctgtca
    47941 tacacctctt cctttccata tagaaaaaag aaagacttga aaggccaatc atatgatcga
    48001 catttcaagt ctctttatta atcgatttta tctacttgat cgaaatcaag ttctgtactg
    48061 gtttcacgtc caaacatatc gatgttgact ttcaattttt ctttttctcc gtcaacttct
    48121 gttacttgtc cttctaatcc agcaaatgcg ccttcgatga ttttcacgac ttcgccaact
    48181 tctacttcta gatcattttg tcgcaggctc ataccgatag aacgcaagat atgattgact
    48241 tcttctggaa gtaatggtgc tggtttgctt ccggctccgt gtgaaccaac gaatcctgtc
    48301 acaccaggtg tgttgcggac aacataccat gaagcgtctg tcatgatcat ttcaacaagt
    48361 acgtatcctg ggaatgtttt atgaacgatt tctttttctt tcccattttt gacttcagtt
    48421 tctttttctt caggaacaac gacacggaaa ataaagtcgg ccattttcat actttgcgca
    48481 cgagattcga tatttgcttt tactttgttt tcatatcctg aatatgtatg caagacatac
    48541 cagttttttt caaaagattc cattgaaggc tcctcatttc attgtaaaat aaaaaaacct
    48601 cagtacaccc gaagtttttc tttcgcttca ttatagcact aaaagtacgt tgtggctagc
    48661 gagttatttt aaaatccagc caaataagct ttggatggct gtatccatta cgtaaaacat
    48721 caatccgaaa ataatagaag tttcgatgac tactaacgta tctttacgga gttgttttct
    48781 gcttggccaa gttacttgtt tgatttcatc ttttacgctt tttagaaatt tcatgagaca
    48841 acctcccttg ctactgttta tttcgtttct ttatgtaatg tatattgatt acaatattta
    48901 caaaacttat tgatttccaa acgttcgccg ctttttcctt cactgactga ttttgtgtag
    48961 ttgcgagacc cacaaacgga acaagcgagt gctgcttttt tctttgccat ttacttctgc
    49021 ccccttgctg agaatcagac ttatacttca taacagtatc acgtaacttt ttacttgtca
    49081 atttgtgaaa taaactatct attgttttgg ataaaattat gatatgatta atttgaaatt
    49141 aagctaaaat gagacaattg gaggtatagc gatgctatta aaaacacttg tatataagaa
    49201 aaaagatctg acaaccgtta cagagactag tacattagaa gaagcattaa aaattttaga
    49261 aacttccgga taccgttgtg tgccgatctt agattcttca ggtaatattt tccgtggaaa
    49321 catttacaaa atgcatatct atcgtcataa agcaaatggc ggtgatatga cgcttcctgt
    49381 cactcatctt ttgaaaaacg ctacaaagtt catttacctg gatacttctt tcttcaaagt
    49441 attctttacg atcaaagagc ttccttatat tgctgtcctt gacagtgaaa atcattttta
    49501 tggtattttg acacatagta cgttgcttaa tatgctctct caatcttgga gcgtagaaca
    49561 aggaagctat gtactgacaa ttgcttccac aggaaagcaa ggagatttgg ctgctattgc
    49621 aaaaatcatt tcaaaacaca gcagcattgc cagctgtatt actcttgata tcgaaaaaga
    49681 cgaatacatt cgacgtacat tgatcacgct accggctaac actgaagcga aagtatgtga
    49741 aacgatcgtc aaccacttag agaaaaagaa ttataaagtg gtagaaattg aaaatttgca
    49801 aaaccaagaa gcataatcaa aaagcgaaaa aagaaaacag ctaatctgct ttcttttttc
    49861 gctttttata attagacacg cttttcaaaa tcaagcgcgc gatctaaata atacatcaat
    49921 ggtgctgaaa cagccatgac gataaactca cttaatgcaa gcgtgccata ggttgtccaa
    49981 aatgcttggc cacctgttgg aactaacata taagcaatca gtcccatgct gatggtaaag
    50041 aagatcgtgt tcaatactaa acgcatcttt acatttttca ctttgttttg caataaagct
    50101 gtcaggccta aagaaagcaa tgtttggccg ccaccaaaaa ccacatccat gataccaaaa
    50161 ccaaagaagg cattatagac gatgaccccg cctaaaactc cccaaagcaa tttacggtta
    50221 aagacaacta aatgattcaa actttctgaa atacgaaact ggatcggccc tgaggataca
    50281 ggcgcaacta aaatagttaa cgctagataa agagccatga tgatgccatt tacaacgatt
    50341 acggataacc gttgtctgct tttcgttttt tgcattttct ctcctctttt ctcctagttt
    50401 ttttaacgcg ggatggttga tgaaccgcgg ttatgtgaaa caaacaataa ttataacaga
    50461 cttttcattt atttcaatcc gtcttatctg cttttaaaaa agcaaaagct taaacaatgt
    50521 aaacgtttgc taaaaatcgt gggatatggt ataaagtaag tgtaaggggt cataagaatt
    50581 cccagtaaag cagacgaagt aggatctgca agaacagaat tgttgtgaaa cttaaataaa
    50641 taaaaaagga tgtgggaatt tgagcaacct cttactagag aacggtctag caagcagctg
    50701 aagatctaag tggacatgcc gaaccttgga acttctcctt acgtaagtaa tggatcatgc
    50761 gcttgctagg cttttttgtg tttttcggtc ttcggacctt tttcattttc ctagacttct
    50821 gctataataa ccaagaatta attttgtaac agattttttt gaaaaaacaa gtttctttca
    50881 ttcaaaaaat agaaaaagct tgctaattat tggttaacca gcaaacagga gtattaaaat
    50941 atcttgtttt attgaatagc aggaggaaat catgaaattc cattatcata tattgatcaa
    51001 tccatcggca ggcagcggaa atggctataa agtagcagaa agaattttac cggtattaaa
    51061 aaataagcat attgactata cacttcatta tagtgaatac aaaggacacg atgctgagat
    51121 tgctgaaact ttggcaaaag aaacattgat tccttggata gaagaagaac agaaaacaat
    51181 cgatactttc ccccttttga tcatcgtagg aggtgacggc accttacatc aggtacttga
    51241 tactttctat cagatggaag tagaattccc agttgcttat attccagcag gttctggaga
    51301 tgattttgct cgaggggcag gccttccaaa aaatcccaaa aaaggacttc agctgatttt
    51361 agcagctcaa tcaccagaaa aagtccacat aatggcttat gaagaaaaaa taagtgaaaa
    51421 aaaaggaata gctgttaata atttcggtat aggattagat gcagctatcg tccatgcgac
    51481 aaatcattct tccacaaaaa agcgattgaa caaatacaac ttaggatcat tttcgtattt
    51541 attctctatt ctccgtgctt tattcactca aaaaggattt ccaattctgg tagatgcagg
    51601 aggaaaacaa ttaagttttt caaacgcatt tttatgtaca gcaacgaagc atcctttttt
    51661 tggcggagga atcgcgattg ttccaactgc tgatgcgtct aaaccagtca ttgattttgt
    51721 cgtggtcgaa cgaatcaatt tctttaaaat cttgtggctc attcttttac ttatacagaa
    51781 aaaacaattg aaatcgaaat atttccatca ctacacaaca aacagattac gtatcgtttc
    51841 aacaacaccg caacatggtc aagaagatgg agaagaaatg gaaaaacgac cttttgatat
    51901 cacaataacc acaactaaac agttgttttg gtgttaataa aagaggtttt gtcagaagct
    51961 acatctggaa caccgtttat tcggaaaaaa ggactgtgac acgacttttg tcacagtcct
    52021 ttttttacta tgcagttaat tctttgccca attgggttgc catcgatagt ttctttcttt
    52081 acccaaaata aattggatca ttgaaaacac cgcagcaaaa gcaactaaga cacaaccatt
    52141 gaaccaaaaa gattctgcaa atgtttgatt gtaactgagt gtttcactga tcaagttatt
    52201 attttgaaca aaccaatatg ttggtgtgaa tgccgctatt ttgctaacca tttctggcaa
    52261 gtattctgct ggtacaaaca ccccaccgat aaagcaactg cctgtgataa aaatatcttt
    52321 gatcccaccg ctggtttctg agttttttac caatgaagtg atacaagccg aaaagctaat
    52381 gatcggcaat aaaaacaaga aagtatttaa aatatgatac cataccgtta tatcccacga
    52441 ggtgtggatc actaacagaa ccattagaag taagaagcca ctgaacaaca gcagcgagta
    52501 actaatcaaa ctaaaggtta ctttgcgatt caagtttcgt ttggtaatgg gcgcactctg
    52561 gtttcgttta ctgatctctt tgcgattaaa tgctagattg atgacgctga aaccgctaaa
    52621 aatggataga aacaacccat aagacaagaa attaaaggtt gtgcccgtca acagcttttt
    52681 catccgctga ctatatcccg cacttagctc aacttctcca gtaaccgaga gtgccatttg
    52741 tgtctgttcc atagctgcat cgattgtttt atcttcttct tgaaacagaa tcagtgtttg
    52801 caaaaaggtg ttcacttgct gtgtaacaac tgttttagaa aaggcatctg ggcgactctg
    52861 tatcttgatc gtcggcatct ttcctacttc gacttgttcg gtaaagtctg caggcagatg
    52921 taagataatg tttacacgat taaaataaag agcatcatcg atttcttttt gtgaggttcc
    52981 atccaatgta acgactgttt ccttgtcatt caaatattta cgaaggccat cgactacagg
    53041 cgactcttct tcactaatca aggcgatttt cgcatcttgc agagaggtat tttcaacatc
    53101 aatattttgc gcaaaaaaga atgtggcaac aacagtgata attacaccta tcagtaaatt
    53161 cgatttattt gctttaagca atgtccaaat cgttttataa agcgtcataa cgcacccctc
    53221 tttccatcca agcatttcct aacgcaaaaa ggaaaataaa tccgatcaac catagaagat
    53281 tggtataaaa actagccaaa tcagtgtaga aataaagttt atacaaactt tcgctgatca
    53341 agttgacgag atttaattga ccaacaatcg gaatatgaag atcaatccaa tacttcactt
    53401 gttccgaccc catcatacca gcaaaaaagc tcatcgcaat cgtcactgaa atcaagatgc
    53461 tatccttttg caccagattc atttttggta tcaaattacc aaccaatgta cctaacaaaa
    53521 tcgcacacaa cgaccccaga gcggtaacaa tcatcaacca ttgccaacgt tcaccaaagt
    53581 ctacttgata gataaaacga aagacagcta acataatata aatctctgtc aaaacaatcg
    53641 taaagcttgc tgccaaatta gcgattgaaa ctaacgattg ctttctagga atcaaggaca
    53701 agcgaatacc atttgacgat tgatttgctt gctgatcctg tacattgcgg accccccaca
    53761 tcgtcccgta gagaatcgcc attccaatca gagtgaagaa ataaaaactt ttgaaactca
    53821 tattacgacc tgcatgaact tcttgaaaag atacttcttg ttcaaaaaca gtcatgatgg
    53881 gcgcgagatc aacgttctcc acttctgcca ctttactcga aattatacca atacgctgta
    53941 aaaattgatc catgacgttt ttcatcaacg tctgactcat tcctgcttga cccacatgga
    54001 gcgaaacaga tgaaccatca atgagataaa aaccagccaa atcatctgca tttagctttt
    54061 cattcgcttc tacttccgat agttcctgta cttcaaataa tggtttatca ttgacttcga
    54121 cttgttctaa gactgtggta aaattttccg aaagcgctgt ttgatccgct ttgattacaa
    54181 ttccagtatt aatcgtttcg agattgccac cttgatcaat ctcgccaaag gccgccataa
    54241 acatcaatcc taaaagaatt ggaaaggcca atgtccaaaa caacatggat ttatttcgca
    54301 gcagtatttt tgtgcgatat ttataaaaat gccaaaacat tatttcccct cctctagtct
    54361 ctcaaatcct tgccagtaat ctctaaaaag acatcattca aggacgcttc tttggttgct
    54421 atttttccgt aactgatctt ttcttcttgt aagattgtaa gaatcggcaa caatactgta
    54481 tttagttcag tagttgttac ttggagctga ccttttttat aagcgattcc atcagaagct
    54541 attgttcgct gacaacacgt tttctgggag taatgggatg tcgatcgtca aacgatccgc
    54601 cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct gtccttgatc
    54661 catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg ttgtatagac
    54721 aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat tccgactttg
    54781 ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt gggcaatccc
    54841 acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac gaaacgaaac
    54901 atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc ttttctcttt
    54961 ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta gttcgtcaaa
    55021 gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat tttcaggagt
    55081 cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag aaagcataca
    55141 gttgatggca gtcgatttcc cactgccgtt tggtcctaac agacctaaga cttctccttc
    55201 ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt tgactaattt
    55261 atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat ctgactataa
    55321 taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca tttgtcacaa
    55381 tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt ctactggtta
    55441 tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc tatttattga
    55501 tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat tggattttcc
    55561 aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt ctgccagcga
    55621 cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg ttaggttttt
    55681 tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc gtaattttgc
    55741 tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt gagaaaaagt
    55801 acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa caaaataaag
    55861 cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa cggaaccgaa
    55921 ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt ttacaaattg
    55981 gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa aatttaagtg
    56041 ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc catgagacat
    56101 cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct tctgttacga
    56161 ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc ttaatggtca
    56221 tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt gttttacatt
    56281 ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc agcccacaaa
    56341 aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa gaacaaaagg
    56401 gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt ttaccaaggg
    56461 agggacacca atgataacag tagtcattat tgagatgatc catttgtgac gaaatcgtta
    56521 caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca ttgtgccaag
    56581 gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    56641 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    56701 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    56761 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    56821 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    56881 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    56941 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    57001 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    57061 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    57121 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    57181 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    57241 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    57301 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    57361 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    57421 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    57481 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    57541 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    57601 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    57661 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    57721 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    57781 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    57841 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    57901 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    57961 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    58021 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    58081 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    58141 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    58201 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    58261 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    58321 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    58381 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    58441 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    58501 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    58561 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    58621 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    58681 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    58741 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    58801 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    58861 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    58921 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    58981 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    59041 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    59101 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    59161 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    59221 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    59281 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    59341 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    59401 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    59461 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    59521 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    59581 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    59641 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    59701 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    59761 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    59821 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    59881 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    59941 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    60001 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    60061 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    60121 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    60181 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    60241 gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc
    60301 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    60361 agtgggccat ctttatgttg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    60421 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    60481 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    60541 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    60601 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    60661 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    60721 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    60781 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    60841 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    60901 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    60961 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    61021 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    61081 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    61141 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    61201 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    61261 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    61321 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    61381 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    61441 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    61501 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    61561 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    61621 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    61681 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    61741 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    61801 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    61861 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    61921 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    61981 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    62041 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    62101 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    62161 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    62221 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    62281 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    62341 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    62401 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    62461 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    62521 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    62581 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    62641 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    62701 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    62761 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    62821 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    62881 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    62941 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    63001 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    63061 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    63121 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    63181 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    63241 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    63301 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    63361 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    63421 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    63481 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    63541 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    63601 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    63661 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    63721 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    63781 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    63841 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    63901 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    63961 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    64021 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    64081 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    64141 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    64201 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    64261 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    64321 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    64381 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    64441 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    64501 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    64561 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    64621 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    64681 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    64741 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    64801 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    64861 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    64921 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    64981 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    65041 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    65101 ctttgcaaaa agcatcaaac cagatgtatc ccgatctagt cgcgtcacca catggattac
    65161 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    65221 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    65281 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    65341 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    65401 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    65461 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    65521 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    65581 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    65641 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    65701 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    65761 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    65821 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    65881 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    65941 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    66001 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    66061 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    66121 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    66181 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    66241 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    66301 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    66361 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    66421 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    66481 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    66541 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    66601 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    66661 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    66721 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    66781 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    66841 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    66901 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    66961 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    67021 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    67081 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    67141 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    67201 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    67261 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    67321 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    67381 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    67441 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    67501 accggaaagc ttacaacaaa acgggctgaa ttccctattg aagaaggact tttagctatt
    67561 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    67621 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    67681 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    67741 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    67801 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    67861 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    67921 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    67981 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    68041 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    68101 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    68161 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    68221 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    68281 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    68341 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    68401 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    68461 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
    68521 aatacgtcga ctcccttaca agagagccga cgtatttttt tcgtttctat tcgagaaaat
    68581 caaaaataga aagccttata aacgatcgat caattcttct aattcattta gacgttgttc
    68641 aaacatcgac attgcatctt caatatatgc agcctgtgtc atatcaactc ctgctttttt
    68701 cattacttcc acaggataat cgctgttacc tgcttttagg taagccaagt agttctctag
    68761 tgcctctggt tcctggttta gtattttctt cgccagtgct gaagcagcag aaaagccagt
    68821 cgagtattgg aaaacatagt aattataata aaaatgcggt atccgtgacc attcgaattt
    68881 aatttctggg tcttcttcga ctgctggacc ataatatttt gcattcaatt taccataact
    68941 atcacttagg tattcactag tcaatggcac acctttttca tcttccgtat gcatgaaatg
    69001 ctcgaattca gcgaattgtg tctggcggaa aactgttcct ttaaacccat ccaagtaatg
    69061 gttgagtaca taagcccgta cacgagggtc tttttctgtt tccaataaat actccgttag
    69121 gatattttca ttcgttgtcg aagcaatttc tgctaaaaag atggagtagt cgccgtacac
    69181 ataaggctgg ttcgaacgag tgaaataact atgaacacta tgtcccattt cgtggacaag
    69241 cgtaaatagc tgatccagcg tatcatgcca attcaataaa atatatggat ttgtgtcata
    69301 gcttcccgaa gaataagcat cgcttcgttt ccctttattt tcgacaacat cgatccaacg
    69361 ttcagagaat gctttttcta cgatggccat gtattcttca cccattggtt tcagtgcttc
    69421 taaagctttt tcttttgctt cttcgtacgt aaaggtaatt ggagcttccc ctaagaccgg
    69481 tgtataaaga tcatacatgt gcagtttttc cacttctaat aaacgtttcc gtaattccat
    69541 gtatcgatgt aacaaaggca aatgcttgtt taccacgtct accaaagtat cgtatacact
    69601 ttcaggaata tgattattgc tcaaagacgc ttctctggca gagctgtaat tacggacttt
    69661 cgctttaaaa ttatgtcctt ttatatgtgt gcctaaagta gaagcaaatg tatttctaaa
    69721 ttgttcgtaa acactgtaca atcccttaaa tgctgcttcg cgcacccttc gatccgtgct
    69781 ttctagcaac tgaccataca cgccatgaga taattggact atttcaccat tttctccttc
    69841 aatcgttgga aaaactagat ctgcattatt caaaacagca aatgtatcac ttgaagcatc
    69901 aaagatttca cctgctccag caagaagaga ttcctgctca gcagataaga catgggctcg
    69961 attatctact atttgctgga tataatggcg atagacttcc aattttggtt cttctttgaa
    70021 atattgccaa atctggtcat ctgacaattg caatatttct ggttcaaacc aggaaacggc
    70081 ttcactaact ttcgaaaaca gactgcttgc tcttgcataa agcgcttggt aatctgtatt
    70141 tccagtgtct tgatcgtttt taaggtgcgc atatacataa atgacttcag tttggcgata
    70201 aaccctcaat acgaattcaa tcgcatttaa aaattgagaa gcgccttgat caagtgttcc
    70261 tttgtgtttt tcagattgtt ttaactcttc tgataattcc aaatatttct catcaaactc
    70321 ttggtcgctt gaaaagatct tggtcaagtc ccaagttaaa ttttcaggca attcttctcg
    70381 ttttggcaac tgctttactt ccatgaaatc cactcctttt cttcccttag ctctatctat
    70441 caaaatagat catagtctaa catccacatg tcacagcatc taacttttct actcattcat
    70501 gtgtctgctt ccatcaattt tagcataact aaatcttgtt ttacatagaa aaccgattcc
    70561 tagcctattt ttatacttac tattttttca aagaagacaa gtgtatgcat tcgttaaaaa
    70621 actgctgata gaccatctct tcatcaatca taggaaaaag ccattccctt ttatgttctt
    70681 ttatttggca aagccatgag ttgaaatcag gaactttctg gttttccaag gcttcttcgt
    70741 acaacattcg ataaagaaaa acctgttctt gtaaataaaa gaaaaaccga cttttttgat
    70801 agatccacga tgatagatgc aaaagatgtt tatgacgcaa gtagcattgt tcttgtatgg
    70861 atactgtatt cttttgcttg tgaataagtt ggcgtgtcag ccatttcttc ccatcttcac
    70921 tcactttttc tatttttttc atttggttca ttttaatttc gttactagtg aatatttctt
    70981 taaggtccct actagaaaaa gtccagcagc tactgcaaaa ggaaatatgc cctgatacag
    71041 tctctgtaat atgatgatac aaataaagct tttgtttctt gagatcagct ttccagcaat
    71101 gcactcctct ttttctatta tagtagcagc aacttttttc tatcaatgag aattgcgatt
    71161 gtccaagaaa agaattacca agtatccacc atacttggta actgttattc aaataatttt
    71221 gggttctttc aataaatctc tggtgagaca gtctagaaca ttggatttct attgctagat
    71281 tcgccaacaa aatatccggc ctttgccgca acctcggcaa ataggcttcc aaaaatactg
    71341 gttctactga ctgtcccagc caatccatga agcattcctt taactgtaag tgctcttttg
    71401 attctctttc actaaagacc gaacagttat ctgatttttg atgggcaaaa tgagcacatt
    71461 ttttctttcc ctttttcaaa atcactcttt ttcgacagcc acgacaataa tatttctttc
    71521 ctttttctgc atgtttagca aaacagcact tattatattc attcattgca agcagcattt
    71581 ttctccctct gaacttattt acgtctaaga atttatttac acgaaaaaag agggcgtgaa
    71641 aaagctattc tgcatcaagt aataagaaca gctaaacaaa aatagttccc aatatttttc
    71701 gtttggttgg gcttgttacc gcagatgcaa gcttttgaac acatttatac gaaaaaaaga
    71761 ctgcgacaca atttttgtca cagtctctta ctataatgct gatttataca aaataaaatt
    71821 tcgttcgctc gatagcatta cgcgccatca gacattgtcc atattcattc aatgtttctg
    71881 gtgtacgatc gctcttttca gcaaactcca aaattcgagc aatctgattc tctacatccg
    71941 cttggttgag attttccatc cagaagtgaa cgactaggta atagcgctct tcgaataaat
    72001 acaaatcagt ccaagcagac tctagttcga catctgttgc aagttggatc acttgttcaa
    72061 aatcttcaaa agcaaaagtt gcttgtcttt cctgttcttc tagggcgctt ccagataatt
    72121 cttcctccgg atcgctgcca ataatctgct gacgaagaaa gtcactcaca tcttctgaat
    72181 caacagtaga atcttctgat tgagaaattt cttcatccgg aagattttta ctgataaaca
    72241 gttctaatcc gtctcctttt ggcaaaactt ggaaagtgac agcttcgctt cctcggaact
    72301 cctcttcgat atcaacttct tccaaaatac tataaaagaa attttcgatt tctttgtgat
    72361 tgccaagtaa atccaaaaat gaaacgcccc gttcctctaa atcttcatga ccaattagta
    72421 cacggatcgt attttcatta atatgttcca tttccatttt gctacacctc actttgccac
    72481 aatgtttaat atagattcat tgtagcgaaa tcagcttaaa tgtaaagaaa aaaaatctcg
    72541 ctgttaagcg aatttaataa aaccagcgga tttttcaacc tttcaagcta tcttatttaa
    72601 ctataacatt tttggcagac gaatactata tagccgactt ttcagctaat tttccctctc
    72661 tatttttcgt gcaatcatca tcttctacta cctttttata catctaatca agaatacttt
    72721 ttgtaaaaac aaaaaaactt ggaaaaacta tctttgtaat agtcttt
//
LOCUS       DACECR010000002        72222 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.002, whole genome shotgun sequence.
ACCESSION   DACECR010000002 DACECR010000000
VERSION     DACECR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.002"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            5..1087
                     /locus_tag="IWO17_000075"
     CDS             5..1087
                     /locus_tag="IWO17_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0288983.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="IWO17_000076"
     CDS             complement(1305..2447)
                     /locus_tag="IWO17_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0288984.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="IWO17_000077"
     CDS             complement(2452..2637)
                     /locus_tag="IWO17_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0288985.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="IWO17_000078"
     CDS             complement(2991..3236)
                     /locus_tag="IWO17_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0288986.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="IWO17_000079"
     CDS             complement(3230..3631)
                     /locus_tag="IWO17_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0288987.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="IWO17_000080"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="IWO17_000080"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ0288988.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..7415)
                     /locus_tag="IWO17_000081"
     CDS             complement(5649..7415)
                     /locus_tag="IWO17_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ0288989.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7428..8222)
                     /locus_tag="IWO17_000082"
     CDS             complement(7428..8222)
                     /locus_tag="IWO17_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0288990.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8232..9608)
                     /locus_tag="IWO17_000083"
     CDS             complement(8232..9608)
                     /locus_tag="IWO17_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0288991.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9728..10375)
                     /gene="eda"
                     /locus_tag="IWO17_000084"
     CDS             complement(9728..10375)
                     /gene="eda"
                     /locus_tag="IWO17_000084"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0288992.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="IWO17_000085"
     CDS             complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="IWO17_000085"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0288993.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="IWO17_000086"
     CDS             complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="IWO17_000086"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0288994.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13042..14673)
                     /locus_tag="IWO17_000087"
     CDS             complement(13042..14673)
                     /locus_tag="IWO17_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0288995.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14977..15648
                     /locus_tag="IWO17_000088"
     CDS             14977..15648
                     /locus_tag="IWO17_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0288996.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(16373..16708)
                     /locus_tag="IWO17_000089"
                     /pseudo
     CDS             complement(16373..16708)
                     /locus_tag="IWO17_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16805..17257)
                     /locus_tag="IWO17_000090"
     CDS             complement(16805..17257)
                     /locus_tag="IWO17_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0288997.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(17264..17812)
                     /locus_tag="IWO17_000091"
     CDS             complement(17264..17812)
                     /locus_tag="IWO17_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0288998.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(17857..18189)
                     /locus_tag="IWO17_000092"
     CDS             complement(17857..18189)
                     /locus_tag="IWO17_000092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0288999.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(18203..18433)
                     /locus_tag="IWO17_000093"
     CDS             complement(18203..18433)
                     /locus_tag="IWO17_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289000.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(18655..19197)
                     /locus_tag="IWO17_000094"
     CDS             complement(18655..19197)
                     /locus_tag="IWO17_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289001.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(19218..20309)
                     /locus_tag="IWO17_000095"
     CDS             complement(19218..20309)
                     /locus_tag="IWO17_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ0289002.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(20317..22527)
                     /locus_tag="IWO17_000096"
     CDS             complement(20317..22527)
                     /locus_tag="IWO17_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289003.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(22543..25071)
                     /locus_tag="IWO17_000097"
     CDS             complement(22543..25071)
                     /locus_tag="IWO17_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0289004.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(25123..25533)
                     /locus_tag="IWO17_000098"
     CDS             complement(25123..25533)
                     /locus_tag="IWO17_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289005.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(25546..25764)
                     /locus_tag="IWO17_000099"
     CDS             complement(25546..25764)
                     /locus_tag="IWO17_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289006.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(25761..26753)
                     /locus_tag="IWO17_000100"
     CDS             complement(25761..26753)
                     /locus_tag="IWO17_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0289007.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(26832..27743)
                     /locus_tag="IWO17_000101"
     CDS             complement(26832..27743)
                     /locus_tag="IWO17_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0289008.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(27762..28106)
                     /locus_tag="IWO17_000102"
     CDS             complement(27762..28106)
                     /locus_tag="IWO17_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289009.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(28126..28682)
                     /locus_tag="IWO17_000103"
                     /pseudo
     CDS             complement(28126..28682)
                     /locus_tag="IWO17_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28763..29263)
                     /locus_tag="IWO17_000104"
     CDS             complement(28763..29263)
                     /locus_tag="IWO17_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289010.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(29264..29809)
                     /locus_tag="IWO17_000105"
     CDS             complement(29264..29809)
                     /locus_tag="IWO17_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289011.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(29806..31062)
                     /locus_tag="IWO17_000106"
     CDS             complement(29806..31062)
                     /locus_tag="IWO17_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0289012.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(31423..31734)
                     /locus_tag="IWO17_000107"
     CDS             complement(31423..31734)
                     /locus_tag="IWO17_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289013.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(31757..33067)
                     /locus_tag="IWO17_000108"
     CDS             complement(31757..33067)
                     /locus_tag="IWO17_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ0289014.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(33681..34133)
                     /locus_tag="IWO17_000109"
     CDS             complement(33681..34133)
                     /locus_tag="IWO17_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289015.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(34135..34500)
                     /locus_tag="IWO17_000110"
     CDS             complement(34135..34500)
                     /locus_tag="IWO17_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289016.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(34599..38882)
                     /locus_tag="IWO17_000111"
     CDS             complement(34599..38882)
                     /locus_tag="IWO17_000111"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAQ0289017.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(39029..39268)
                     /locus_tag="IWO17_000112"
     CDS             complement(39029..39268)
                     /locus_tag="IWO17_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289018.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(39358..39630)
                     /locus_tag="IWO17_000113"
     CDS             complement(39358..39630)
                     /locus_tag="IWO17_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289019.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(39713..39964)
                     /locus_tag="IWO17_000114"
     CDS             complement(39713..39964)
                     /locus_tag="IWO17_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289020.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(40960..41871)
                     /locus_tag="IWO17_000115"
     CDS             complement(40960..41871)
                     /locus_tag="IWO17_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0289021.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            complement(42067..43902)
                     /gene="lepA"
                     /locus_tag="IWO17_000116"
     CDS             complement(42067..43902)
                     /gene="lepA"
                     /locus_tag="IWO17_000116"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ0289022.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            44049..45269
                     /locus_tag="IWO17_000117"
     CDS             44049..45269
                     /locus_tag="IWO17_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0289023.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            45289..45783
                     /locus_tag="IWO17_000118"
     CDS             45289..45783
                     /locus_tag="IWO17_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0289024.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            45776..46447
                     /locus_tag="IWO17_000119"
     CDS             45776..46447
                     /locus_tag="IWO17_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289025.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(46598..49207)
                     /gene="clpB"
                     /locus_tag="IWO17_000120"
     CDS             complement(46598..49207)
                     /gene="clpB"
                     /locus_tag="IWO17_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ0289026.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(49458..50504)
                     /locus_tag="IWO17_000121"
     CDS             complement(49458..50504)
                     /locus_tag="IWO17_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ0289027.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(50515..50787)
                     /locus_tag="IWO17_000122"
     CDS             complement(50515..50787)
                     /locus_tag="IWO17_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ0289028.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(50822..51730)
                     /locus_tag="IWO17_000123"
     CDS             complement(50822..51730)
                     /locus_tag="IWO17_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ0289029.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(51862..53094)
                     /gene="pepT"
                     /locus_tag="IWO17_000124"
     CDS             complement(51862..53094)
                     /gene="pepT"
                     /locus_tag="IWO17_000124"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ0289030.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(53109..54230)
                     /locus_tag="IWO17_000125"
     CDS             complement(53109..54230)
                     /locus_tag="IWO17_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ0289031.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(54227..54928)
                     /locus_tag="IWO17_000126"
     CDS             complement(54227..54928)
                     /locus_tag="IWO17_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ0289032.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            complement(55072..55512)
                     /locus_tag="IWO17_000127"
     CDS             complement(55072..55512)
                     /locus_tag="IWO17_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0289033.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(55641..56567)
                     /gene="dnaI"
                     /locus_tag="IWO17_000128"
     CDS             complement(55641..56567)
                     /gene="dnaI"
                     /locus_tag="IWO17_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ0289034.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(56567..57940)
                     /locus_tag="IWO17_000129"
     CDS             complement(56567..57940)
                     /locus_tag="IWO17_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ0289035.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(57963..58454)
                     /gene="nrdR"
                     /locus_tag="IWO17_000130"
     CDS             complement(57963..58454)
                     /gene="nrdR"
                     /locus_tag="IWO17_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ0289036.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(58766..59098)
                     /locus_tag="IWO17_000131"
     CDS             complement(58766..59098)
                     /locus_tag="IWO17_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0289037.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(59150..59779)
                     /locus_tag="IWO17_000132"
     CDS             complement(59150..59779)
                     /locus_tag="IWO17_000132"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ0289038.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            complement(59743..60579)
                     /gene="mutM"
                     /locus_tag="IWO17_000133"
     CDS             complement(59743..60579)
                     /gene="mutM"
                     /locus_tag="IWO17_000133"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ0289039.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(60624..63269)
                     /gene="polA"
                     /locus_tag="IWO17_000134"
     CDS             complement(60624..63269)
                     /gene="polA"
                     /locus_tag="IWO17_000134"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ0289040.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(63395..64078)
                     /locus_tag="IWO17_000135"
     CDS             complement(63395..64078)
                     /locus_tag="IWO17_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ0289041.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(64071..64556)
                     /locus_tag="IWO17_000136"
     CDS             complement(64071..64556)
                     /locus_tag="IWO17_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ0289042.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(64863..66197)
                     /locus_tag="IWO17_000137"
     CDS             complement(64863..66197)
                     /locus_tag="IWO17_000137"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ0289043.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(66328..67089)
                     /locus_tag="IWO17_000138"
     CDS             complement(66328..67089)
                     /locus_tag="IWO17_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0289044.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(67105..67587)
                     /locus_tag="IWO17_000139"
     CDS             complement(67105..67587)
                     /locus_tag="IWO17_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ0289045.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(67607..69787)
                     /locus_tag="IWO17_000140"
     CDS             complement(67607..69787)
                     /locus_tag="IWO17_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ0289046.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(69931..70662)
                     /locus_tag="IWO17_000141"
     CDS             complement(69931..70662)
                     /locus_tag="IWO17_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ0289047.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(70731..71672)
                     /locus_tag="IWO17_000142"
     CDS             complement(70731..71672)
                     /locus_tag="IWO17_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ0289048.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            complement(71852..72220)
                     /locus_tag="IWO17_000143"
     CDS             complement(71852..72220)
                     /locus_tag="IWO17_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0289049.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT        21124 a        15163 c        11247 g        24688 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggtac aataggccaa atatcttgtt tatctttaac taatgtaatt
     6241 gcttggtcag ccacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccaga
     6301 gcttcttgct taggtaataa aatttgttct tttgcttttt tgtgtaatcc caatttagct
     6361 tttaacccta aaattctttt taaggcatct aataaccgat catcagtgat aattccttta
     6421 cgatagcctt ccatcatata attaaagtct tcatctggat cattaaaaaa tagaaataga
     6481 tcacatccag ctgcaataga agtgggcaat aattctgatc gtttcattgc cccagtcatt
     6541 cctaacatat gactagcatc agtaactact aatccattaa atccgagttt tcctctaagt
     6601 aagtcagtga ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca
     6661 ggattaaaat gttttgaata agctggcata tggatatgac ctgccataat tgaaggtaat
     6721 ccagcttcta ttaagcctga gtatactttt ccaaaagaat tatcccattc ttccgtacct
     6781 aaactattaa tactaaaaga tagatgctga tctcgttcgt caataccgtc accagggaaa
     6841 tgtttagctg caggcaaaat tccactctcc tgaatacctt tcatataagc taaactaagt
     6901 tctaaaactt tatcagggtt ttgtccaaaa gatcgactag aaatgatagg atttctccaa
     6961 tttctattaa tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca
     7021 atcccagaaa ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt
     7081 acttctacac caacttcagt accatctgta caagctccgt taccacctgc ttccgtatta
     7141 gcagcaatta atagaggaat tttactttct gtttgtaaga tatagttttg atcatatacc
     7201 tcatcagcag ttcctggatt atatcgtaca gctccaatat ggtactgatt gaccatatta
     7261 ctcaagtaat ttttatcacg actagatccc atattaacaa acaattgacc tattttttct
     7321 tccaaagtca tagaatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatta
     7381 tatggttttt ttcgtaaatc tacttttata gtcattttct ttctcctcta cttattagta
     7441 agatttttcc aaaaagtatc tatatatttt tgttcaatat ctacataatc tcccgataaa
     7501 catttaaata aaacatcagt tcttaagttt tcccatgatt ctttttcttt attgaccaaa
     7561 ataggataaa agaaaacttt atttttattt gcagcttcta gatcaccagg agaatcacca
     7621 atcattaata tcttatccaa atcacaacca ttttcaatta cacttcgaat aatatcttct
     7681 ttttttcctt tatcttgaca acacagttca tcaactaatg ataataattc atgtctttcc
     7741 cattcttctt gaacagcttc cttattagct gagcttacaa taattatctt cccatatttt
     7801 ttcagagctt ttatagtatc tctagcactt aaaaagggtt tatctaatcc ttttgcttga
     7861 ctaatctgtt gattgacttc ttccgaccat tttaaagctt ttaataaatc ctctgactga
     7921 gaatattcta tttccctctt caaagatgga ttagacaatt cttttgttag agatacccag
     7981 tgctttaatc gattaatcga tgttacatca tttttttcta atgttttaac taaacctaaa
     8041 aatctgttta ttccacgagt ctctgaatat aaattaatat tattccaatc ctgtaggaat
     8101 ttttctctat gatgaacatc aaaaatctct gcagcaattg gtccgaaaaa gagattgtgt
     8161 ttataagtca tcgtatccat aacacagcca tctgaatcaa tacaaaatat ccaattattc
     8221 atatttctct cctatttatg tttttgtttt aaaagctcgc aattttctaa gactttatct
     8281 ttagataatg gataaaggaa aaataaaatt aatcctacaa tcagaaaaca aattgatgga
     8341 attaatgttg aagcgttata taacttttct aaaacatctc ccgtttgtac atttgctaaa
     8401 gagtcatagc ctataataga aagagtccaa cctccgacgc ctccagctag agcttgacct
     8461 atttttctag caaaagaata taccgcatat acggtcccat cttctctttg atcagacttt
     8521 acttcttgat catcaataac atcagtaata tttgcccaaa taacagtatt aaacacaccc
     8581 aaacccatat agccaaccga actaagaatt aagaatacat acatattact agcttttatc
     8641 aaaaataaga tcaaatatac tattgctgaa aagaacattc ctactgatgc cagttctttt
     8701 tttccatatt ttttacttaa aaacaaagtt aaaggcgaaa caactattaa aaccaaaata
     8761 ggattgataa aattcattaa aactattccc tgtgcatcat tatagtaatc aggaaataca
     8821 taattattca tactcataat taataattga gcaaggagta agaaaatagc cgcaccaatt
     8881 atagaaagta atgccctatt tttaactaac ccttttaaag attttaacaa tgaaactttg
     8941 tattgagatt ggtcaattct tactcgttca gtagttagtt tataacatat taaataacaa
     9001 ataagtgcac atatcgaaaa tattcccgca acaattgtaa aagttgatcc accacgaact
     9061 atttgagcgc ctgaatcatc tcttgtataa ataaataatg gtgttaatac accaatgacc
     9121 aaacttgcta aagtagctcc aacatttcgg aatgtcgata gcattgttct ttcttctgta
     9181 atagaagtaa tagctgatgc cattgatcca taaggaatat ttatagatgt gtaaaaaata
     9241 ctcccccata atatatacgt aacatacatg tatactacct taacgctcat agaagctgaa
     9301 atcatagaag tttgatacat taaaaaactt gctaaagcga ctggaccact cattcttaaa
     9361 atccacggtt taaacttacc ctgcttagtt gctttcgatc tatcaacaat acgccccata
     9421 gttacatcag taactgcatc gataaatctt gctactaaaa ataatgttcc aacggcagct
     9481 ccgcttatcc ccaatacttt tgtataaaag atcattaaaa aagaactagc aaaaatgaaa
     9541 gtaaaatcgt ttccaaaatc tccaaacata tatcctattt tgtctcttat accaaatttt
     9601 ctttccatac aattctctcc cttctgttgt taagcgtttt cattctgatg aaagtacgta
     9661 ctatactctg aagcgtaaag agtcagtatt tattttttac atttcagagt attatttgtt
     9721 ttttctccta ataattcttt atctcataaa acttatctat atatttacta gctactcgac
     9781 ttacttcttc aaaatcacct ttttcaacag aagccaataa attcccacct acaccaactg
     9841 caacaactcc tacttcaaac caatcttcta agttctctat acttactccg ccagtaggca
     9901 ttatatttac gtgtggtaat ggagcttgat aggcttttac aatggatgga ccaaaagcag
     9961 aacctgggaa tagtttaatg atatctacgc cagcagtaag tgcctccttt atctcagtaa
    10021 ttgtcataca acctggtaaa taaggaattt gatataaatt acaaatctca gctgtttgtc
    10081 gatcaaaaga tggactaacg atatattctg ccccagccat aattgcgata cgagcagtaa
    10141 acgcatctaa aaccgtaccg gctccgatta ttacatcttt ttgatcagca tattttttag
    10201 caatttcttt gattatttct tcagcattag gaactgtaaa agttagttca atccctttaa
    10261 ttccacccgc aataattgaa tttattgctt taatggcctc atccttattt ttcccacgta
    10321 caaccgcaat gactccagcc gcttctaact tacttaaaat ttctactttt ttcatatcta
    10381 tctccttcct ttatggatac atactttgtc tagctgtact aacttacaaa gaataaattt
    10441 aagcttttat ttcaagtatt attttctact tccgatttca aaaaaattaa aataattata
    10501 cgcattacga taacaaatat tctccactat ctgactaaga tacatttcat catctggaat
    10561 tcggcctgct ttaacccact cgcttagtaa ttgacataat actcgacgaa aatactcatg
    10621 gcgaggatag cttagaaaac ttcgagaatc agtcaacata cctgtaaaat tcgacaacaa
    10681 actttgttgt gccatcgtag tcaactgttg acgcattcct tcagctgtat cattgaacca
    10741 ccaagcacat cccaattgga ttttttgaat cataccccct tggaagcttt gcattgctgt
    10801 tgctagtgga agccaatcag ttggatttaa tgaatataaa attgtttttg gtaagcaccc
    10861 actcttttca gcggtatcga acaatgctac aacattttgt gtcaaactag cttgatcccc
    10921 catcgaatca ccaccgacat ctactccaat ttgttccttt aatatacttg aattatttct
    10981 taaagcattt aaatgcattt gcatcgtcca accatatctt gtatactcag acatcaaatg
    11041 taattgaata cctgtaacaa attgtgcaat ttctgataaa tggaaatccg tttttcctgc
    11101 taaagctttt attaacagat tgtcgagtat tttttcatct gctatttcat aaaaataagt
    11161 attcaaaccg tgatctgcta atcgacagcc aatttgatga aaatgatcca ctcgttggct
    11221 caatgcttgc ttcaacccat taaagtcttt gattggtata gccgctgttt gactaagttt
    11281 taaaatatag ttcgaaaagc ctgatgattt gatactccag accttatctg gacggaaagt
    11341 aggtaatacc ttgaaagaat tatcctgttt agaaagctca gtatgatacc acaagtcatc
    11401 tgtaggatca tccgttgtac agatggcttt cacatccatc atctccaaca attctttcgg
    11461 gcgataggaa tccgtttgaa ttttttcgtt agcacgtcgc caaatttctg gtgcattctt
    11521 ctcagaaatt gtcaaatcaa tatcaaagta acgttgtaat tccaaatgag accattcaaa
    11581 aataggatta cctggcgcct tctccactgt ttttacaaaa gctaaaaatt tttcataatc
    11641 atcacctgga ccactgatca actcttcttc tataccatta gctcgcatca atcgccattt
    11701 ataatgatct ccagctcctt tatcatttaa ccatacctga gtcaaattct tgtattgctt
    11761 attttctagc aaatatgctg ggtctaaatg acaatgatag tcgataattg gttggtgttt
    11821 tgcaaattta tgaaaaagtc ttttagctat ttcattctct aataaaaagt tcttatctaa
    11881 aaacatattt aaacactcct ttccttccgt tagtttttag aagagaaaat ttttcctcta
    11941 aataaactcc cttatataat tatttactcg cccccttttc aagcgcttcc caaataccat
    12001 ttaaataaac tgcacctaga gctcggtcat ataatccata accaggtcta ccggtttctc
    12061 cccaaatcat ccgaccatga tctggacgaa tatagccatt aaatccatta tcgtacattg
    12121 tttttaatat cttatacata tctagagaac catattctga tttatgcggc gcttcgtaaa
    12181 aatcgagtcc attgtcactt aaatatttga tattacgaac atgtgcaaaa ggaatccgat
    12241 cccgttgaca aaattcagat agaatttcat agacatcatt atctggtcgt tctgcaatac
    12301 ttccagtaca taatgtaata ccattagaag gagaatccac tacattacac aaccaatcta
    12361 aatcgtcacg atttttaaca atacgaggta atccgaaaat ggaatatggt ggatcatctg
    12421 ggtgaactgc cattttaatc ccaacttctt cacatgtcgg gataattgct ttgagaaaat
    12481 atgcaaagtt ctctcgtaat ttctcttcat caacttcttt ataggcttct aataattgct
    12541 taatttgtga taaccgatga ggttcccaac ccggcaattc aaaattatta gtagaatttt
    12601 ctacttctct aataatttcc tccggatcag tagggatatt ttttgaaata taggctaacg
    12661 tagaagaccc atcatctaaa cgataatcca aatccgattt tacccaatca aatactggca
    12721 taaaattata acagataacc tcaatcccaa attctgccaa attgcgaatc gtctgtttat
    12781 aattctcaat gtattgatct cgtgttggta aaccaatttt gatatcatca tgaacattaa
    12841 cactctcaat aactttcaag gtaagtccat tggcttcgac ttcatctttt aaagatttaa
    12901 ttcgttctct cggccaaacc tctccggctg gtatatccat taatgttcca acaattcctt
    12961 tcattgctgg gatctgacga atttcttgta gtgagatttt ttcttcattt gccccatacc
    13021 atctaaaagt catttccata gttactttcc tccaacttct gttatatatt tattgattgt
    13081 tttacgaact gcttgaggac ctactagcat ttcgcaaaag attttttcag ttttctttcc
    13141 taccccaact tgatataaat ctaaaccaaa gatttcttta ttttctaaaa tttcctgtac
    13201 cgcacaatga atctttgaag gatcttgttc acctaatttc aatactgata atttatcctg
    13261 caactctacc aataacggat caggacttgg cacaaaattt tccccttgat catcgactgc
    13321 cagtaaataa cgtaaccaag cagcaatagc gataggaata aatgttagat tttctgcatt
    13381 tccgtctttt tcgacatact ttttaattgt ttcaccatag cgaatcgcta ctttttgaga
    13441 agtatctgtt gcaattcgtt gtggtgtatc tggaatatag ggattaggca aacgtttatt
    13501 aattacttca tcaataaatt gttttggatc aataatgcct ggattttcaa ctactggtaa
    13561 accttctaaa taacctattc ctttgattaa accaaccaaa tcatcatcct gcatctctgc
    13621 tgaaattgat tgatatccta gcagacaacc tgttactgcc aaagcagtat gaagtggatt
    13681 caagcttgtc gtaaccttca tgatgtctgc tttatcaacc gtctctctat ctgtcataat
    13741 cacaccagcc ttttccaatt taggccggcc atttggaaaa ctatcttcaa taactaaata
    13801 atgaactacc tcagtattag tgaaagcagc aatattggta tgtttttttg tgtgaatgat
    13861 atctaaatcc tcgagcccca tcatcaatag atcttccttt acattctctg aaggatttgg
    13921 cgtaattcga tcaatcatac tccaaggaaa agtcactttt tctttatcct ttaaataagc
    13981 aataaatttt tcagaaacat atccttcttt ttgccaagct tctgcaatcg taaaaatact
    14041 atcttgaaaa cgttgaccat tctgtgagaa attatcagta gaaaccatcg caatcggcaa
    14101 ctctccagca ttaaatctgc tatataacaa ggatgttaca atactcatcg tatgttgagg
    14161 attgctaggt ccagatttga tatcagcttt gactaccgaa aagaattgcc ctttagaatc
    14221 ttttaaactg taaccttttt cagtaatagt aaacgttgta aactgtaatt taggatcttc
    14281 aaaatattta accactcttt gatagtccgc tatattcttc ggattgcaat ataaactatc
    14341 agccgttact gccaaaagtc tttttttgag ttcaccatct ccactcataa caatttccaa
    14401 aatgtcattg ttatagtcat gataagcttt ttccactact tcttcatcaa atgtttcaca
    14461 cacaactact cctgtttcta attcattttg atctgctaag cattgagcaa tttctgcatg
    14521 aaatcctcga taaagatttc ctccaccaaa atgaagccat actggatgtt gctggccttc
    14581 ttgcttaact tctttgacat caaaacacgg cagtagaatc tgcatttcat tgaattcttc
    14641 gatagaattt tgccaatttt taatcgaaac cataattaac ctcctcaaac tgatatgtta
    14701 gttttgatac attttatatt ttaaaaccca ttgtaaccgt tgtcaatata aaaagggaat
    14761 aaacttgctt ttttctacca tatcagaatg attgtatttt ttattcagaa aaataaaata
    14821 ctccttcttt ttggactgat tttatcgaaa cttatttttt ctttttttca tgtattcttt
    14881 tatttttata actagtgtat tacttcaaaa tttgatatta tatggcatga acagaattaa
    14941 aatatcagtt aaaataaaag ttaaggagca ttgtgtatga caattaaaac tagtctccaa
    15001 aatcaagctt accaatctat tcgccgacaa attatctatg ctgacttaga acctggaaaa
    15061 aaagtatcag aaaaaaaatt agaagaaaca ctttctattg gtcgtacacc aatacgtgaa
    15121 gcactgattc aactccgaca acaaggtctt gttgatacaa ttcctcaaag cggaaattat
    15181 atctcattaa ttgatttaga ttcggccaaa aatgctcgat ttgtcagaga acatttggaa
    15241 cgacaaatta tgttagagtg ttgtgccaaa atgaataacc aaaaaaaacg gattttagaa
    15301 actataattt cagaacaaga aaaagctgtt agtcaaaaaa atgctcgtag tttttttcat
    15361 aatgataatt tattccataa agcttgtttt gaaattgcag agcgagaaga agtatggcaa
    15421 tggttagaaa ataacaatac acacttagaa cgatttcgat ggctacgagt aaaaacggaa
    15481 ggattaaagt gggaaaaaat tatggaacaa cattatcaac tatttgaggc attaacaaaa
    15541 caagatccag aagaagctaa ctttctaaca tcgttgcatc ttcatttaat gcttaatgag
    15601 caaaatattg ttttagctac tcatccaaaa tatttcaaaa atatataact tttaactctt
    15661 tttaaattaa atggttgttc tgatcagaaa aatatcgctt gttttcttat tatgtaatat
    15721 atttcataaa ctattctgca taacaactat tgtttgtact ttttacattg gataactaat
    15781 tatgactgct gataactttt gatattaaac tagtatctcc aatacaaata aaaaatagat
    15841 atcttgttca tcaaaattag ttcgtggata cttaccttca tttacatcat tcagcggttt
    15901 cccattttct tttattctat tatttcattt tctaatagac ttggcttcat ttcgcaatac
    15961 tttttttcaa taggcatcaa attggtgttg tttagatatt tagttgtagt tttgtgacat
    16021 gataagtatg ctcctttttc tcttttccac taatacaacg atcaaagggt gcttttttca
    16081 aaaaactaga aaaatcaaaa taagattcgt attttgattc tatatatcct gtttcaacta
    16141 catcttatat ttcacaatag aataaatagc cttttgcata gctgcttaac ttataagaaa
    16201 ttgcaattta cttcttcaaa gtgaaaacga atatgagtaa aataaaatta ttcttcttac
    16261 agataattaa aaatagcgcg taacaaaaat cggcattcaa tcacataatt aggattgatg
    16321 ccgattactt atattctacg tatagtttta gtattcttat tttttatcac cagtaaccaa
    16381 ttgagaacag ccctaaagca gaatagtctt ttatctcaaa acgcttaatt tcaggcttag
    16441 gaatatgaat aatcttttga gtatattctt tctcttttaa tcttactgaa gaaataccac
    16501 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
    16561 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
    16621 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
    16681 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
    16741 gacacagcat taagagatat accaacacaa cagtgcaaat aactctacat cttccttcgc
    16801 aaccttattt caataaacca taaaaattat tttttaatag aaactgaaaa ttaaaactag
    16861 aaaaagtagt tttaccgaat gtaagagcat catcaccatc aatatagaaa tacattcttt
    16921 tccagttaca tttaattttt aaacaaaatt ctccccaaaa aggaactttt tctagtgaaa
    16981 acttggtaat ctctttgtaa ttaattcttg ttaaagaata attaaacact tcatttttac
    17041 ttgttatttt ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat
    17101 cgacaaacag taaatgatat aatccatttc cacctccaag catattcaat actggtgaac
    17161 atgccttaga aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt
    17221 gtttaaaagc catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc
    17281 tagcagcagc tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag
    17341 cttttcctaa ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa
    17401 aagcttttaa tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc
    17461 caggaagaga attaatggct acacatccag catatcgttt ccaatcaact ttctccattc
    17521 taggaacaat aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac
    17581 tgattcgttc acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa
    17641 aaccaactgt atcaaaataa aaagttaatt catctactaa tttattcttt atttctaaat
    17701 caactgtatt cactttttct gtggcataca cacttgttcc agtactaaca aaagtactta
    17761 aacctaataa gaaaacacct aaaacaccaa atattttaat tattcttttc ataatttctc
    17821 ctccataaaa aataagttac tttttaaata tcttgtttat gaattcattc cccaaaggcc
    17881 ttttaagatt gatacagcag tccgttcact tcttttagac ttgtcataca agtcttttaa
    17941 atgatcccat atcagactag tttgtctatc tagcttcaat ccattaaacg ctaatgaacg
    18001 gttcattttt tctattactt tagataaagc tacagaaatt tttatacctt cacaacgcgc
    18061 atcttggatt tcaataatag tatcatcaat tagtttttga atttcttgcc tatcaacata
    18121 ttcaatctta gaaagagaat cttttaaagc tactaatgat tcaacgattt cttttttctc
    18181 ttcagacatt ttcttcctcc ttctagtgac ccgtaaagcc atgtagagca gagtccattg
    18241 aaccagtaat tacactatct acaacgccat tccaatttac tctacatttt ccatttttca
    18301 gatttgtcgg tccatacgga tagccttttt gacattcaat gttcggatta taccagatag
    18361 gattatacca ttcagccgca gaagcagatt cagtcgatac aaacaatccc acaccaacaa
    18421 atccaataca catcacacta tagccaatta gttttttcat ctcgtatcca tctcctaaaa
    18481 aatagaatag ttcttatagt tattcataag acatattttt tccgtttttg ttttatcctt
    18541 tctgtcctat aacaactttt ttcaagtaaa gaatgacata gtttttctta aaaaaacagt
    18601 ctgttttttt ctatttcttc ggtgtataat aaatatgagt gttcattttt tattttaata
    18661 cgcactccgt acataataaa tcttgatcgc atcaatcttt tgagcttctt gatctacttt
    18721 caattgaagt aatacaaatt gagtaacctt attcattgtt tcttctccaa ataacaaata
    18781 ttgatccgga ttctctatat cttgtgtcac ctggtaaagt ttcccagttg ttttcatttg
    18841 tacatgcggg tctacatcaa tactattgtc tttaatacaa cgatctgtca tatagtccct
    18901 cacagcttga ttgcgtttat aaacatctgt gaaattactc cattttctag caaaatcttt
    18961 taagaaattt tttgtcattt catctatttg catttgttgc gctgtctcgt cttctaatga
    19021 accaatttga ctgttacttt cagtactttg aagagttcca cttgtttctt ttactgcttc
    19081 gattgtttct tctgtcgttc tcaattgtgt tttttgtttt tcagaagaaa accaatgaca
    19141 accagtaaga cctatcaaac ataaccaact aaatactaaa attatccctt ttttcacgtt
    19201 tctagtcact gcctttctta tttcacctta cgaaccagac tacgaatttt tgtttgattg
    19261 tacgtgcgtt gataccgagc cataatccgt ccttgctcgg cattctgttc cacactagag
    19321 aaatgttgtc cgccatatga aacatctgtt ataattccgg tgtgaccata gattccagga
    19381 gacaatgcac cacctgcttg ccaattcacc acatctcctg cttgtaaatc acttggtttt
    19441 gggttcatga ttacttgcca accataggtt gtccagttat aatcttcccc tattaacatc
    19501 gcaaacatgt gtccagaacc cattaactgt gggccaccta attgagatac ataccatgcc
    19561 gtaccaccat aacattcccc attatttacg gatcgtccaa tgatccgatc caaaaccttc
    19621 gcattgcctt tactttttcc tgcaataagc gaagtttttt ttgttggtag gctattccaa
    19681 tgcttgatcg caaaagtatc atattccgtt aagttatagg acgttatgat actcattgct
    19741 cctcgataat acggactccc ttcggtaata cctgctgccg catagcctcc acgacctaga
    19801 atcaataaac aacttccagg atctttattg cccacacatt gattaaagtt gctttggctc
    19861 gccaccataa agccataata gtcataacaa gcttttaagt tcggaaactt catatagaaa
    19921 ccaccttcag aaccaccaat tccacgtgca gaaccttgtg ggtagggaca accaggaaac
    19981 caggtaatcc caaaaaagtt cgtatcgttt cttccagatt gtgactgtcc ataagctgat
    20041 tcatagccta attgagaaag aatcacactc ggtaataact gatactttac agctgcacca
    20101 actgctgctt gcgcatgttc atctgtgatt ggaccacgtc ctttaatctc tgaaaaaatc
    20161 ggtaatcctt cggtatattc cccattaaaa gcgacagcta gacctgtagc ttctcctcca
    20221 tgctcagaaa gttctcctga aaaaaagaat ggcaatagaa aaaagggaag aataaagaaa
    20281 agaagacaac ctagacaccc tttcttcatt tcttcctcat cctttcttcg ataatggttt
    20341 ttggactgtt cgtccttttg gtaaagtaaa atcagttcct ggttttgcgt ccaaaatcgg
    20401 cagtgtttta ggatcctttt ctattttttc cgattgctct gttggcttat ctaaagaaga
    20461 aaatgcaggt tgtaacgatt ttggtgcgga aatcgacggt ttactttcag acgaacgttg
    20521 ccccggttgt tgcacagaac gatttcctga tagagtggct accgacgaat taggaacaga
    20581 gtattggttc aataactgct gcattgctgg ataactccca tttttcgcct gatttttcac
    20641 ttggcgaatc tgttgtgaaa gagaaccagc agctgttaat tgtggcacgg gtacttttcc
    20701 agaaggcaac ggaatcgatt gaccaacaaa agcgatacct ttatttaaca atggttttct
    20761 ttgattattc ccttgttgct tactcaattg tgccaatgat tgtacctgtt gttttggtct
    20821 tactgaattc atggacgaat ttggaatcaa accatttgaa agaccagaat tccactctgt
    20881 tttcgatttg gtagaagaca tttcaggctc taccccatga taacgaacat gacgaccact
    20941 ataaggcaag gcttgatttg aacgcccacc tagttcaaag ggattaacca cttgcctccc
    21001 ttttaccgat tcttgtggtg tacgtttttg ttgaaacttt tgaaatccat gtatcgctgc
    21061 agaagcgagt ggattcgttg ctcgatacac attccctgct aatcctgcac ctttgattgc
    21121 caaattttgt ccagcagttg atagttgacc aggcagttgt ttcatttgag acaaagaatg
    21181 acttgctatg tttttcgtgt tgtttatagt ttgatctgca gctcctaacc ctagtttctt
    21241 taataaatct ttccgtttta caatgagtaa aattgatagg agtgctttga caaacatatt
    21301 tcctaaaaaa ccaaaaacag tattcattgg aattaaatcg tctactatgt tatagatcaa
    21361 aaatgccacc aaaataatca cactgtaaaa agatttttga aacatcaagc ccaaaaagga
    21421 ctttaaggta ttcatgagca tataatcaaa tactggaaca aaagaagcga gtaatgaaaa
    21481 aggaatcaca agtaataaac acaacaccaa catttgaaag ataaaacgaa tcatgccaat
    21541 cactacaagt ggtaatccaa tacttagatt aagcaatggc accatagaac caacgactgc
    21601 tttataaaca gctttagttg gttgaatata gacacgataa gccttctttt tccctctatc
    21661 ttctcctgca gcagtggtca cttttgtctc aatatcagat aattcttcac ttgacgcttc
    21721 cttggctaga aattcttgtg gttcaatctc tgcttgatac aattcttcta aatttgttgt
    21781 cccataattt aacaatatat acggatcaac aacagccgct ttataataaa gttggcgcac
    21841 tttttgaatg gcttcctttt gtgtcgttgg cactaaaaca ccattgacat cttttgtcgc
    21901 caattcatct agactatttg tagcagaaaa aataaagcct tctacctcca cggacaaatc
    21961 atttaaccct ttggtaaaag cttctccttt actaggtacg ttactaccat agccaaacca
    22021 aaccattgaa aaaatgacaa cgaagagaaa tcgaataaac accatttttg cgtaatgttt
    22081 ccctctaaat atacgaagag taaatagata taacacatac aagacgacaa tccccgcacc
    22141 aataggatcg tatatttttg aataaatagt atccgaaaaa gtgaaaaact ggtgaattaa
    22201 ctcgttcatt gcagctcctt catagagctt ctcaatgaca aaatcaaggc cttgccaaat
    22261 caatttactt aaactaaaga acatattcgt catcattttt agaaaatctg tgccatttgc
    22321 cccaaataac attttgccta atgaaaattc actatcagat aaataagaag aaaaggtatc
    22381 aatatgaaat ttcccaatct catagatcga ttgatccacc tgcttttctg ccgcatacac
    22441 caatccacca acaaaaagca gtccgaatcc aatcgttaaa aaaaacaaaa cgcaccattt
    22501 cttttcctta tgctttatat aagccattgt ttcacctcat ttctattgca tgattaacgt
    22561 atcctgttct tctacttgag agtgacttgt tttttccgtt gtttgtaatg cagcggtcat
    22621 ttcttcaaac aaactgtgta cattgagttt tcctacacgt ccatagggat ctaacatcca
    22681 acactgtcct ttaatcatgt ggctcatttc agctaagttc gcttcattta cttccaaacg
    22741 taaatgattc aaaatctttt ctcgctcact gtcttcatca aaagcaaaga cgacaccaaa
    22801 attccctttt tctttatctg aacctaagtc accaataaat tgtgtatcta acactaattg
    22861 attcatctgg gaacgcccca cacgtgccat tgctgcaata atactacgtc ctgcgttcga
    22921 attattaaat acccatgctt ctgtaaaata aatcgctgtt ttcttactac tatctcgttt
    22981 tccaaacatt tcacaaaaac gacctaaact aatcatcaaa cacaaagact ttcgatccgc
    23041 atctgtatat agttctggtc ggacattgtc atttggtaat ttcaaatctt ggatctctaa
    23101 aatcgttgtt ttggcattaa agtctaatcc cgggttttct ccataagaaa atcctaaacg
    23161 taaaatcgaa tcttctaccg tcaagcgtaa aaactcacca tattctcgaa cctctttttg
    23221 ttccatttgt tctaaatagt cgaccacatg catcaagcca acttgttgtc cggcttcttt
    23281 ttgaactaaa acttcttgga ttccctttaa taccgcccct tttaaaaggt gattggtacc
    23341 taacggactt aactcatcaa tcatatcctg ggctacttgt ttcgcatcta ctcgggataa
    23401 acaaacaatt ggatctaata caccataatt acggggattt gtagcatcta acgtgacata
    23461 actcattttt tgcaaatgtt ctacaaacaa agggtaatgt ttctgataat acgggtcttg
    23521 aatgagtttc gtaaaccatt tgcgcttttc tcctttggga tccacaaata aactttgtac
    23581 attcataaac gaactataca aaaagagcaa gccaactaaa aaggtcttcc ctttaccggt
    23641 ttttccagtt actgctacat gaggcgcatc aaacaacgca cctttgattc cttccgcaat
    23701 ggctagaaga ttcaaaaaga caaaacgatt tgacgcataa attaattctt ctaatggtgt
    23761 tttttctcct cgagaaccac catcccctaa tgtatcttgc cgaccaatat accagccgga
    23821 ttccatacct aattctgata ctgctgaaaa caaccattcg ccaactgctt ctacagttgt
    23881 tcggtgcttc caatgtaact gccgtcccaa tggttgccct aataaacatt tatagaacaa
    23941 ttctcgttga tcattttttg cacggtggat atggataatc gtatgaagtt gttgaataac
    24001 cgttgcacta ttttgacgaa cttcttcaac cgtttgaccg tatacaataa aacatgcgat
    24061 ccaatcgacg acaggttgcc cattatctac tcgttgcttt aaatgtttta ctaatcctcg
    24121 aacagttaat aagcgttcac taggttcttc atcattttga accaattcct tatgctctgt
    24181 tttgaaccgt tccgatagcc aattcagttt taaaccaata cctccctgtc ctttcaagcg
    24241 tggatagtga attttcattc gcaattctgc tgggaaagaa agatcctgag caaattgaaa
    24301 tagatcacaa tttctcatat tttcaggtgt ttccccaata ggcaacaatg ctaaataact
    24361 actccctaac tcatcttcta attgaagaag gcctttatgt tccttcggta tacgtacata
    24421 cgttccttga ttgtctttca gtacttccca attattttgt tcttctactt tgtgagccat
    24481 tcctcgaata tattgcagac gattgagata caacagctct ttttttacta agggacgaat
    24541 agaaaagcta taaagtaatt gtgtcacttc ttttacataa ggatcacttt cagaaaacaa
    24601 agtttccaaa tcatctactt ctaatccaaa taagcgtgct aaacttcctg ctaaatctaa
    24661 ccagattaga tttattacat catgaaaact ctccaccgtt gctttttctt tgactttgat
    24721 tcctaaaata aatgaaggac gatagacttg accttctcgt tccaacaatg tcactgtttc
    24781 ttttgcataa tacgcaccaa ttgcttgact atcgggagaa aatccctttc ctaatgtgtc
    24841 aaacttcgtc cgtaaattca ttgttcgtgg atacatcacc aactccacat cttcataatc
    24901 tgctaaaagc aacagtaaac gagccatcat ttcttttatt tcctgtgtct tttttggtgt
    24961 gttttttgaa attgttcgtg gaaaaacatg atagtaggcc cagatacaat cctctccatt
    25021 taacaaacaa tttttcttga atgcttttac tggttctttt acaagcacca atgaatttca
    25081 cctcccaata aaaaaggaac aaccagttga ttgtcccttt ttctaatcct ctaaataaac
    25141 atacttattc acaaaaagta attcttccaa tgcattgttt gataccttgt cccagttata
    25201 atacacacaa tcaccattca tcatacgaat catataacaa aaccaatccc ataaataaaa
    25261 atgcactttt tttccatcag gttttagttt tgccagtgct aacacacata aataggggag
    25321 tcctatataa aaaacaactc ctgctggtgg aaaaaaagaa atgacccaac caataaatga
    25381 acgtaaaaat aaatattcaa agattacgac caacaaaaaa gttgctgcat tgactaatgc
    25441 aactggaaat ggaagacgcc aattgcttgt aatctgttgt acgcgataag gttccgcata
    25501 aacaatcgaa taatcataag gttcccaatc cacttcacta tcctcctaat ccataaataa
    25561 tttttcaatg attttaccaa acgcatcaaa tacacgtgtt ggatcattta agaagaaata
    25621 aactgcggca cctgctgcga tagatattaa tgcatttcct acttttgatt tcataaaatt
    25681 tcgcaatccc agataaacaa ccaccattat taaaaaatag ccaccttgcg ttaatataaa
    25741 atctacaatt cccttcagtg tcatttccgt tcttctcctt tcctatccaa ccaattatgt
    25801 gttaattttt caacataata ctgatttgtt tttttcgtaa tgacaatcgt catttgttct
    25861 ttttgtaaaa gtgacgtatc accatctcga aagacgaccg ttgcttttac aataaaaggt
    25921 tttttcgttt ctttataacg caatactttc atctctactt gatcaacttt agctgtttga
    25981 ttgagtcctt caggttcttt catcatgtat gacatatctt ttgcattgtt tttcgcatac
    26041 ttatctaaga aatcttccac aaatccttca acatctttcg cttccgacgc atcaaccgtt
    26101 tcgtataatg tgtcatcata agtaatcatt cgttgttttt ctgtattctt tggtagtgcc
    26161 gtaaaataag gataagcaat aatacgacag cccttgtttg aaatagaaaa gggaagattg
    26221 agtacttgtg tgattgtcac acttgtttct ttcttactct ctggattcat cgagcgaata
    26281 tcataagtta ctttataact agcagttgga atggtttcaa tttcttttaa tccaataaac
    26341 gaagcagcag ttaattttcg aacacctgaa acacttcctg gttcttgaat aaagttttgt
    26401 gcataaaact ctttttttaa tgtttcttga cgttgttgtt cttgttcatg attatcgaat
    26461 gtattcatat acaaagcaac aaaacgattc ataaaggatt caatttcagg tgtgtacgtt
    26521 aattctgacc gactttgtac ttctttcttt tgtaattgtt ggatttcatc tttcaaaaac
    26581 gacacttgaa ccttcgtttt catggattga aaaaatgcca agccccctga aaaacaaaga
    26641 aataaaacca aacaccaaat ccatttcact gctttttttg aatggatttt cttttgcttc
    26701 ggtactttct ctgtataaac tttttctttc cgccgtagtt gtcgtttttt cataagctac
    26761 tccttctagt atgtttttac tagtacgtac gattgacaac gataagcttg tttttctgaa
    26821 aaatagtcca ttcaattacc ctcattttct attccttttc tctttctttt atatccaaca
    26881 aatccagcaa tacttgtcaa cactcctcct agaacagaaa gtgcaacatg tatttcttca
    26941 ccagtctttg gtaagacttt tgtttttcca tctgctgttt tgatttcaat attgccactt
    27001 ggaagtttct ttacaggaat cgacaaatcc tttaccaaat tcccttcact atcttggatt
    27061 aatggaatac catcttttac accaacaatt gtttgatcac ctgctacttt taatggggtg
    27121 gttggtgttg gaatatgggt caattcctct acattactag tgacaatcgg tacactcatt
    27181 ccttgagaag tattcgtcgt aatttcaccg cttggggtta ccgatactgt gacttctttt
    27241 gctggaacaa taggtttatt ttcttttgga attgcttctg atttttcaac aggttgtttc
    27301 ggttcttctt tttgttcttc actggtagac gacatcgttg gctgttccgt acttgacgaa
    27361 tcagtattct tttcagaaga actggttgtt cctgattgtt ccggagttgt gactatactt
    27421 gaatccgttg ttgttgaact atgcacacct tcttcttcga tcgaatcagt cggtaaagtt
    27481 gattcaggaa actctggttc tggtgtcact ggctcttttt caggtttctc tgactgcaat
    27541 tgttcaggtt ctgctggttt ttccgttgtt cctccattta gctctgtctc aacatctggt
    27601 aatccttccc ctggcaatgt ttctacaatt tctgacttag gtgtttctac agtttcatct
    27661 gcataaacca atgaactccc taattgaaac gaaaataatg aaattcctag tgttgttacg
    27721 actgctaatg ttgttttttt catgattatt cctccaattt tttatgcaat tcgtgttttg
    27781 ttcatagacg ccgcactaat attatctgaa cgataaaaga actcccgtcc gttttcttct
    27841 aacccaacat atggattatt tttgaataat tgaatcaata gttcactggt gttgtatttt
    27901 ggatagtttg caaaaaaata atactgtgct tgtcgcccat ccgaaaattc aacaagtaat
    27961 tcataacgat accgcttcaa tccttttcgt tgttttaaag gatctctata ccaacttgtc
    28021 catcgttcca ttcgtggata gagatagaca cgatacacat agaacaatgc cataccacat
    28081 accaccaaaa ataataatcg ccacataaaa attccccctt ttcttctacc tttcttgaat
    28141 catcgaacat tcataccctc tatagagatt tgaaaaatcc atcatcaatt gttcatataa
    28201 acgttgttgc aaatagcgat tgtttcgaat acataattct tcatagccat caatcacttg
    28261 atcttctttt tcatagcatc ttgtagctct atgacgctca gcttgcatga cataaccaaa
    28321 ctctgcacca tatcgttcta ctgttaataa ctgttgacta cgcaataatg caatataaca
    28381 atcacaaaaa tgttcaaccg acatttgatt tttctcacgt ttgtgaaata ggtattcttt
    28441 agaaagcaca ttattcgtta aacaatctga taaatagcga taatctggcg gtcggttatt
    28501 ttcatctgtg aatttttccc ataataaact gatttttatt cgttcagcgg ctagatagtc
    28561 ttgttcgcgt ggataaaatt catcaatcgc acaaagtttt ttatcacctg aataaacaaa
    28621 tccatataat gcgaaacgaa ctcttccttt tcaattggct ctcctaaaaa ttccagtgac
    28681 ataccatctt ctccttccta aaaaaaataa aaaagcagaa accgatttgg cttctacttt
    28741 tctttccttt tatagctctt ttttacggaa gaatccgcaa atgacatttt ttcaaaaaat
    28801 caatcggcac aagttcacga aaacttggag caattttgga tagaatcact tccccatctt
    28861 gtaaaataca atcataaaaa tcatcagtta cttgatactt acgcttctta caataattga
    28921 taaaatagta gcgatcattt tctatttcca ttcgttgttc tactaagcgc atacgaacat
    28981 catcatagaa ataagccaca aatcgccctt caagttcatg tttttcaatg ggatactctt
    29041 ctggtaacaa tctatttttt cgtcgtttat acattgctgg ataccttaga agaagttcat
    29101 ctaaacgtct gtcagtatat ggtaactgac gaatcaacga ttctaatcgt tctttataca
    29161 gatagaataa ttggcgcaag caatagtgta ccgcctctgg attttctaat actgttggat
    29221 cacgtgacca atacgcacca acataaatca taattttttt catttagaca atcacaccag
    29281 caatactaat gaccgatcca caataggtag aacaagtggc atagcgacca atttcctcta
    29341 attctatctc aatctcttct aaagaaagat atgtctgttc ttctgttggc tggcgactca
    29401 tttcccaagt gacagtcacc aatgaaattt cttccataag aggtttttcg ctgctaagtg
    29461 ctgccacaca gttttcatat atcttcattt tatcgcagcc ttttttaacc gctaaaatcg
    29521 caggtattgg caatgaaaat cgataaaagc gaatgaattc tagccttcct ttccttcggc
    29581 taaattccaa ctcttcgagt tcttgttcgt atcccttacg aacttctaat aagcgtgcaa
    29641 tttcttgcca gttctctttt tcctgataac gtccagcttt taccgcttgt tcaatataag
    29701 gaactcgaaa acgaaaataa gacaaatcaa ccatcttttc atttaatgct tcgattgcag
    29761 cctttaaatc caacaggcgc agccattctg ttgtcgtata tgcattcatg caattttccc
    29821 tcctgacgtt cccatccatt ctagatagtt ttgttgctcg tcatttagtt cggcattatc
    29881 aatcccttct tgtacaaagt ctcttcctaa aatacgatct gcttcttgta acaactttaa
    29941 tgtcggagct acttgtttca aaaaccagcg ttctgaacga tctaatgaga atttttctgg
    30001 ctttactgta aaatccaatt cttccatcga acgaaataat tttgcccatc gtttatcaac
    30061 aatccgtgca tcactttcca gtctatccca aacaataagt tttgcattaa ttaaaccaac
    30121 gccaatctct cctaaacgtt tcggtgaatc acctaaaata aatgcttcta ctgcttgtgt
    30181 tgctttctca ttggctaacc gtaactcata tcgattataa ataccatatt tcgttaatgc
    30241 ttctaattct gaaatttttt ctcggttcgc aatttcaaaa cgtttctcat aaaaattaaa
    30301 aaagagagga ctctttcgcg atccaaaata caaagataat ccttcacttc gaaaatagcc
    30361 atcatctaaa aaacaaccgc caatattctg gaatctctta aagtccattt ctaataaacc
    30421 tgctttttgt ttttctagta attcaaagag atcgaaatgt tcttccgttt ccaatcccca
    30481 aagttcatct aacgcaatat cgaatcggct acattgcaca cgtgtataac cagcttccaa
    30541 ctttccttga tttttccaaa tggaatatag ccatgttcgc caatctgttt gttgttcacg
    30601 catgacttcc tcatattccc ggcaaccttg tcctgaaaat tgcacaagta cattttctgt
    30661 tacagttcgt tttgaatgcc agaacaccca gatattccca cgaacagaaa gacagtcaaa
    30721 aagatacaag cccgttggac gtgtttcaaa ttctgtcaga tccattcgta atacacgttg
    30781 aatcactgtt ttgtagtctg tcccaaaaaa ggaaagacgc aaataatcaa tcattgctcg
    30841 aagatctatt ccgcttttca tatacaatcg aaagcgttcc ttgattcgtt gttcaaaatc
    30901 ctctgggatt tttcttttcc cttgttccat ctttctaatg taatcttttg agcagcccat
    30961 caattgcgca aatcttgctt gtgatagcgg ctttttatct ccacttatat tatgaattgc
    31021 acttaacttc tttctgtagc tttttagctc aatcggtgtc attttctcgc tccttttata
    31081 aaataacaaa atcgattttt tttaaccctt tagaaacacc gatatatcaa ctagtccccc
    31141 tcgttttttg ggaaaagtag tacaactgct gaccttagat aatcacttga tacaaaaggg
    31201 tttataaaac ttttttctat gtgttatgtt atttctgccc cccatattat atatcggggg
    31261 gctttacaca aagaggccgt gcgcaagcgc acgacctctt tgtgagctgc acacgctgct
    31321 agcgagtgcg cattataggc ccatgcttcg cagccttaat gcgcaccgcc aggcagagtg
    31381 cggctaccag actacgtcta gagccctccc gatagataat aactacaaaa ttaaatgatc
    31441 taattttgtt atttcactat atttttcagg agaaaaaacg cgataaattg acaaagttaa
    31501 atcctcttct aaatctactc gataatgata caatcgagtc aatcgttcta tatctttttt
    31561 cgttagcaat acttgattaa taacttttcc tgtaaaaaca cgttctttgc gcaaaaagcg
    31621 gtaatcagtt acttgttcaa tacgattcaa ccaatcataa aacgttctgg aagaaagaga
    31681 aggattaatc attcgtattg ctgtagctac tctataataa caactctctg ccatttaact
    31741 tcacctcctt agttgattac aaaatatgcg catcagaaaa acttgttcct tttggatctt
    31801 ttgctaaata catttggcgc tctagttgtt cttgtaacaa ctgattatca tctcccatcg
    31861 tttgatcttg actatcctct tttactgtaa aatcatagac aatcatttca gtaaatttct
    31921 caaattcttt gataaagtga taatctttgt aaacaaatgg cgcaaaaaac tctacaggta
    31981 catcaaatcc tggaaaagca tatccacgac caggttgatc tttgttgtaa aatggcttat
    32041 ttttaaaatc ttctccaaaa gtcattaaat aaccaatggg agacaatcga ccaacagaaa
    32101 tacggaatgt taaattatcc cgaacaccac ttggaaaata ttctgcatct ggtcgttgca
    32161 ttgctaaaat aatgtacatc cctgcttgac gaccctttaa tactaatggc tgaatcaatt
    32221 tatcgactct atttctcgct tccatatcca acgaaccata aaaagctgcc cattcatcaa
    32281 aaaagataaa atgcggtggt aacccataat aagcataatt tttccctggt tcatatttag
    32341 gatctttcat ggttttcata taggcatacc gttgattcat ttcttttaat gcatttcgta
    32401 aacaaataat catttcttca tttattttgt aaaacacatg tcctttgaat acatcgacgt
    32461 cacctaatgc tgctaaatct gccgcttttc catcgcaaat atctactgtc ccaacactta
    32521 aacaagcttg aattaaagaa tacaataaat aggttttccc accaccgatt gcaccagcaa
    32581 tcaacatatt tggtgattga tcatataccc aatccaaagt tttcattaaa tgaatacgtc
    32641 cattttctgc tatacattga tcaataggaa tccgaccaga atctgaaagg aatacataat
    32701 tgattcgggc tttttctcgt tcttcacgaa ttaaatcacg caaaaatact tcagataaca
    32761 aatgacccat ttttaaaaat cgttcgtgaa aacgcccacc atctaaagga aaagaaacca
    32821 ctaattcttg tttcttttga taaaaataaa cttttggtaa tctcattttt tcccttgttt
    32881 gtttctcact aacttgtttc tttgtataaa caaaccgatt gtcaattaac attcgaacaa
    32941 gtaactgacg ccaataaaca gaggcaaaat atccttcttt tagtcgcgac caatgcatca
    33001 accctaaaat taacccacta accagcaaca aatacaacac aatacttatt ccaggaataa
    33061 gatacatttg ccaattcgaa aacgtactta aacgtggata aaatactttc caactaaacc
    33121 aacctaacag aatcaaaaat ggaaacaaaa agaaaccata atagcaaaac cataaataac
    33181 gattgaaata gcgaattcgc cgaccacgat acttgaaaaa atgagctttc aattgtcaca
    33241 ttcctccttt ctgtttttac ttttcctgtg ctaaaaactt ttgacaaaaa gcaactaata
    33301 caggaatatc acatttagta aaaaaacata atcgaaaaag atttaatcga tttttataag
    33361 ggtgtgcaca catattacat attcgtacta tacgaatcca aatcacttcc tgatgatctt
    33421 gtttccactg gtacggtaat gaaaaacgaa caagatcacg taaaaacata cactgcccaa
    33481 taaccgcaca aatatatgca tttgtttgta caaaaatcgt tggattgtct ccaaaatact
    33541 caatcaattg ttggatattt tgataacaag cacaatctct caaagtattt gattggtttt
    33601 atctgaattc aaaatctccc cttccttcta tcttagataa aaagaatagg aagactcctt
    33661 cctattcttt caaaaaaacc ttattttttc tctttttcct tagaatcaac acttggttgt
    33721 ttttgatcta aacctttttc ttgtgattgt ttcatattag gattatttgt tggagcttct
    33781 actttctcta atcctttcgc ataaatcact aaatctgaat attcacgatt gccatcttgt
    33841 gctggaatat ataccacatg atattctgga tcaattaatt tcacaggatc acgtcgttta
    33901 aaaccagtca aatctacatc tgctggaaat ttaactcgtc gtgcaatctt tggttcatct
    33961 ggatttccaa aaactaattg tgctcgctgt gatcgaattt ctcccgtata atcgcctgtt
    34021 tcattatcaa atacacgatc agactcttct aatgcgccaa agcgtacggc tcctaaacaa
    34081 gtttcatcta atgcttctgt aatgtcataa ttatttggaa attttaatcc catcttacaa
    34141 aacctcccca atttttacac cagttaaaaa gacaccaaca tcttgatagc cagttgttcg
    34201 acgctctgtt tgtcccatat catttgtttc acgactattc actcgttctt tacgaaaaac
    34261 aaaataagct tcttcaaatg aaatacgcac ctctttttct ggtaatcgta atcttttatc
    34321 ctcttcagct gtgagataaa caaaaaaagc aggctgttct tctcgcgaac actctgcttg
    34381 taattgaaca tatcgcccag acacaccatt aatttccttt ggtggattag gttcccgtga
    34441 acccaataaa aatacttgac ctaaaaactc tttcttcata cgcaatgctt tttctgccat
    34501 aaagtttttc ctcctcttaa aaaccaaaat aaattctaaa tatttctaca ttacaatcaa
    34561 acaagcggtt aaaaatccta accgcttgtt cctcatgact aatctttggc tttacgtact
    34621 ttataaagtg ttatacctaa agcaatgact aatactacta atccaagaac cataagtata
    34681 ctacttgttt gttcacctgt ttttggcaac attggtacaa cagtagctgg tttaacccgt
    34741 tgttctggtt tcactggttg cggtggtttt ggcgcttcca caatttgaac cgtttgatct
    34801 ttgtttgtta gatctacatt gtgtttcgcc acttcttcaa acgtttcttt ctcatcttcc
    34861 gttttcgctt ggagccattc cgcaaaactc aagccttttc ctgcaagatt gcgaccgtcc
    34921 actaagaatt caaataagaa ttccatcatt tctttatcgg ccgcaaagac tttttccgca
    34981 tgagtgcctt caaatggtgt gccgtctggt gttaagtagc cttgaacaat taagcgatag
    35041 atttgaccaa tcgcaagacc gtctactttg acccattcaa gcacagtttg atttggcaag
    35101 gccaagatct cttttccgcc atttttattc gtcgctttcg tcgtaatggt tggattactg
    35161 attttgatgg tttggtcatt gtcggtaata tctgcgtgag aagccaataa tacgccttct
    35221 ctaaacagat cttcaaagac cacgacttct ttgcctcgca acgcacgtgc gtctagaata
    35281 aagtctagat tgactgtacc atccgttgtt tctgccacaa acgttaaggt acttgtgact
    35341 tcttttccat ctactagcac tggtttttcc gttactttgt ccattagaac accgttgact
    35401 gtgtatgttt ttccaacgac caaatcgtgg tatttcacag cgtctgtaat ggtgactttc
    35461 gttaatggat taacctcttt caagccagtg ggtttatgtg tagccgttgt acgtacactt
    35521 gggttagtta ctcgtaccgt ttgccctttg tcgttgatgt ctttatggtt tgctagttct
    35581 tcatcatttc taaaaagact ttcaaacacg acgatttcac gaccatttat acctgtcaaa
    35641 tcaaaggtaa atggtacgat caccgtttca ttttctgctt tggcaataaa gccaacactc
    35701 gtttctaccg tatttccatg cacaagcaag ggttcaccgg ttgctttatc cattaagatc
    35761 cctttgacca agtatgcttt tcctggaaga atctcggtaa tttcaacggt atcgtttaag
    35821 gtaaacaatc ctgttgggtc tgcgactgtc aatccgtctt caaagacagc cgtggtatga
    35881 acggttggat tattcacttt cacactttgg gcttcatcgt tgatatccgc atgtgctgca
    35941 acttcaatgc catcacggaa aagtttttca aaaacaacga tcgtttgacc tttcagaaca
    36001 ctagcattaa ccacaaattc aaggtctttt gtgccattga ctttgtctgc aataaaggcc
    36061 aaggtacttg tgatttcttt gccatcttgt aaaattggcg cattcgttgc cttgttcatc
    36121 aatactcctg aaatggtgta ttcttttccg acaaacaagt tggtataaga aacactgtct
    36181 ttaatgacga tcgtttctaa tggatcaacc actttttcac catcggcttt atttgacgct
    36241 tgtgtttcta gttttggtgg aatcaagtaa ttacgtacag aaaccactaa cgtttcccca
    36301 tcatctggcg cattttgttt gaatgcaacc gtgaatgctt gtttatctgt atttggttgg
    36361 aaactttcta atggggcatt ttccaagaag taatagtcat cggcaactag acctttcact
    36421 aaaatttccc cgttttgatc agtggtatat tcaccaattg cttgattttt accgttatac
    36481 aaggtaaagt tcacacctgc gagcggcgtt ttcttcttgt tccattcttc atacttgaat
    36541 aattttacac ttttccgtgc ttgacggttc gcaaaatgga tcactttgat cggttctgta
    36601 tcattggtaa tcgagaacac taatgggttt tgattcaaaa cgtaatttac gggggctttg
    36661 atttcttcca aacgatagcg atcttttcca taagtaagtg taccgttagt taggaaaaag
    36721 cctttttcat tggttttaaa gatatccgtt gtttctgtat catttggaat ctccattgtg
    36781 acataacgat tggctaaagt atcaaaaatc ttgaattctg tattagcaag tggaatcact
    36841 tttcctgttt cttgatccac tttcaccact ttaagacgtt gttcaattac tttatcttct
    36901 aaaacataat ggaaagtttg cccattttca gaaattgtga cttcaagtgg ttcaaatagt
    36961 ttataccctt cttttccttt ggtttcagaa agaatgtacg tatcaaagac aacggtattt
    37021 ttaccaaaag tagcataacc atcattgtct gtcacaacgg ttttcaccac tttgccagtt
    37081 gtttttgacg tcaaagtgat ttcaatgcca gctaattttg gtttgttatc tggattcgtt
    37141 gaatcgacta aatctttatt agcaactttc actaaatcaa tggttccttt gatcacttga
    37201 tcttttgcgg tcgcattgac agtttttcca ggttcaacaa caatttcaat tgcttcatca
    37261 gaaagcacat aaccttttgg cgctttgatt tcttttaaaa catatttgcc aggatcaaga
    37321 tctttaattt ctgcctgtac tttgccgtta atttttttca aggtaatttc tttaatcttt
    37381 ttcccatctg cttgatacaa accatagacc gcattttcta aagaagctgc accttgtgct
    37441 ttcacaccag tttccgcatc ttctttaaac acacgaatgt ttccttgttt tagaatttgt
    37501 aaattaataa cagacgcatt aggttctgtt ggatgtaaca caccaattgt ttgagctcct
    37561 ggacgacgat aggctaatgg cgcaccttga actttaattt tattcaattg aatttttgct
    37621 ttatccggtg cctcttttgt gactgtaatc catacttcat tgccgtgaat agatacattg
    37681 acaccttttg gtttttgaat gtctttcaca tatttcaaaa aatcattttt attctttact
    37741 ttcaacgttt cgccaatttt taccgtatgt gtttgattat taaaagttgc tttttcacca
    37801 aagttattaa tcaaacgttg aatttctgct tttcgacgat cataatcaac acggatattt
    37861 ttgattggga tttttgtgcc caaagcttcc caaatcataa attgtgccgc aatgtattgt
    37921 tcatcggatt tgtctttatt tgtgacatat cctaaataag agatcaagct tgtttttttc
    37981 cgtacatttg aatttttcaa atagtttttc aaagcagtag ctgtataaac ttgaccttct
    38041 gcggctgaga caccaggctc aatacaaaac gccacttgtc cattaatctc aaaacgaacc
    38101 atataattta aagtgaagtt tagattgttc ggactagaag taacaccaat attttcgccc
    38161 caatcaattt tccgaccacc taaaacagct gcattggttg cacggcgaac acgaaccgaa
    38221 cgattggttg gtactgttac taacagtgta ggtgtacctg tttttggaat gttctgctct
    38281 tttttctttt gattttcaat actagccgta gattctttgt ttggtttcgt tggttgattt
    38341 tgactagctg cggttgaaga gattgttttt tcaacgtttt cctcattttt tccttctttg
    38401 tcagcagtag ccgctggttg ttccactgtt tcttctattc gtttcttttc ttccgcttgt
    38461 ttctcagaag actctggctg cacagaaggc gttgcttcgg cagctttttc tccttctaga
    38521 gtggacgttt ctgaagcagg ggttgtaatt gaggcagttt cttcaactgg ttgtgaagta
    38581 ccaggactat tccctgggtg ttgtgtttcc ccaatcgtct gatcgagagt cgttttggaa
    38641 ttgaccgttg gcgcttctgt cacaggggcc tgttcaacag tggcttcacc agctgggacc
    38701 ccggcttctg ctggttggac ttctgtcact tcagaagcga cttgggcaat ggcttccatc
    38761 gtaggttgta tagccgtcgt ttcttctgca tgtgcttgag cagttcccac caagaaacag
    38821 aattgagaaa aagccaccaa tgcgagtgct ttcaaccaat tcattttcgt ttttgcatac
    38881 acaagaattc ctccttttcc tgagaatttt atataataaa acaaaacgcc gttaccaaaa
    38941 gtgataacga cgtgaactgt ctcttctatt tttatctggc gctcttatct gaacacattt
    39001 catcaatgat tttaccctcc cactataatt acagaacaaa aaaagaacct gcttcgtgga
    39061 aatcaactgt ccatttccca attggaatac cagctttttt ggcaacttca ccatcaccaa
    39121 atctcatgcc ataccaatta ctaccttcat ccttaatttc tttccaacca tatgacatag
    39181 cttcatctaa tgaatcaaaa atcatgacac tattgccaat tgttacagga tctggcttaa
    39241 gatcttctgg aagagaaata tcaatcatac cataatgatc cgattcctca gtattattgt
    39301 cattactagc tactggtttg gttgttctac tggcttattt tgatacaaca agtactctta
    39361 ttcttctttt acttctccgt tttcaatttc ccattcaaac cactccgcaa agtcttgctt
    39421 aaaaccatct actcgatacc tattttgtaa ataggcatct tctgacgtcg taggatctaa
    39481 ataggtaggt ttattctctg gacgagcata gacacctacc catgtttcgt aatctccttc
    39541 aaaataacta tctaccaaat agcctttttt tctaagaatc tcccgaactt gatttttaat
    39601 tattgatact tgttcaaaac catcactcat tctatcacct caaataactc tagattctca
    39661 taaatattcc cgacaatttc aatcctatca atgatctcta acaaatcgct acttatgctt
    39721 ccccattcgt ataagaattt cccttccgca aaataaattt tccataaact tcttcatgat
    39781 cgtcccaacc aatatcactc tcgaaaaatt ccacgttgtt cttatctttt agctcagtaa
    39841 atgccataag ttcaacatgt tttccaacct cgaaacaatc acaccaccca tcaaccgtta
    39901 cacgtgtgcc ttcaatacct aagaaacttt gtcctcgtgt attaaacact aaaactttcc
    39961 gcattttttg ctcattaatt aatcacgcgc gaaatctagg aatcacaact tattcgcttc
    40021 ttctttataa ataaaaaaac tccaataatc ggaaattact atcttttcct cattcctttt
    40081 ttaaatcttc atacttcttc ttatctttgt cctaacctaa taacacaaat tttgaagaaa
    40141 aactcagtaa aacctgattc tttttttatc ttcacaacaa ccaacttttt cttcagatcc
    40201 ttttactaga acttgtatac aaaaattgtt gaatgctgat ataatttgcg gctacttgaa
    40261 aaaacgatag atgtaaaaag ataaatagaa tcatttttta ttctatatca gtctatgatt
    40321 cattgcttat caatcaccca acccattcga tgccgttttt tttagagaaa tttaaaaaag
    40381 accgtacgaa tctcgcatta tccgattact tttccagaaa agatccccaa ttctgtataa
    40441 ataaccctat cttcttgttg gtttcatttt ctcatataag aattcagtca atctacccac
    40501 tatgaccatt actacgtcca acacaactga catattaacc acgtaagtat tccctcacag
    40561 atctccgaat ctccatggta tggattccca tcctgacgct gcagaggaca acatatttca
    40621 tgtggctctg tacccttttg atttggaaac actacaaatc aatactcatc tataacttat
    40681 caagcgaact ttcgctaggc ttctagtgat tactaaattg ttggataaca tacatagccc
    40741 attttatcta gctgttattc ataacagtta gttcttgtat tacttcttcc gcttgtgttg
    40801 ccattgagct tgtcaaaaag ttacttagaa aaaagctgcc agtaaagcaa ctttcaacaa
    40861 accctttttg tttcttatca tatcaagaaa cctcctttta taaatttttt ctataataaa
    40921 aaacgtcgat aacttaataa gtatcgacgt aaactatttt tatatcccgt aaatcaaaaa
    40981 ttctaaagaa agatttccta tataagcata ttggataatt cgattagtat tgggtatatc
    41041 tctaccattc tcatacctat ctatggacca ttttgaaatt gaaggagaaa aaagctttcc
    41101 aaactcctcc ctttctagat aataatcttt cctaattttc ctcatcctta atcccatact
    41161 aaaagaattt atctttttaa aatcgctaac tctttcatct tttggaagta tattcttata
    41221 atcttctccg tacataaggt aaggaacagt gaccttacca agataggcaa tattcattag
    41281 tttttcaata tctggaatat taataccttt ttcccatcta cttataacat ttttgcctgt
    41341 atagatggta aagtttttac caaatttttc aagcgtctct tcccggttct ttcggacttc
    41401 tcgtattctt tttcccataa caattttatc gtcttttgat aaactaaata tctcactatc
    41461 acgattaatc tccttcaaaa tttctttttc attacttaat agaaaaatat cgattttttt
    41521 ttgcatttct tttgacggat ttctctttct atttaacaca taactgagta atacttcact
    41581 aattcctata ttatcagcta actctttgtt aagtaactta ttcatatctc tgtatgcaat
    41641 tagtttaccg atgagaatct caatcttatc ttcctcatct tttattagag tttctatatt
    41701 cagaatcttg cttagagtat tatctacgtc tttagggatt tttcttgacc ctttttcata
    41761 tttaactaaa gtactttgtg aaatacctgc taaatcagct aacttttttt gagatagttt
    41821 tttctctttt cttagagatt taagaaccct tccacttgaa ttatcaacca tgaaaaaagc
    41881 acctcctatg agatgctttc atcatatact gacagctaaa aatcttcaag aaaaatattc
    41941 caattttgtt aattttagta tcgccgtgat aaccaaatga cagtttaaaa tcttttatac
    42001 agataatcct cagtgttgca tctactcaaa aaggtttatt taataggttt ttcgattgat
    42061 tataagttat ttcacactat cttgctcatc cattttcaga acggccatga aggcttcttg
    42121 cggaacttcg acggagccga tttgtttcat ccgcttcttc ccttcttttt gtttctctaa
    42181 gagtttacgt ttacgagaaa catctccacc ataacattta gccaagacgt ttttacgcaa
    42241 ggctttgatg tctgaacggg caacgatttt ttgtccaata gctgcttgga ttggcacctc
    42301 aaattgttgg cgtgggatta gttttttcag tttttcaacg attgcttttc cacgttcata
    42361 ggcaaagtct cggtgaacga taaagcttaa tgcatcgact ttttctccat tcagcaagat
    42421 gtccatcttc actaacttgc ttttttgata accagacatt tcataatcca atgaggcata
    42481 gcctttcgtg cttgatttca atttgtcgaa aaagtcaaag acaatttcag aaagcggaat
    42541 attatagacg acatttactc ggtaatcatc taaataatcc atcgtaatga attctccccg
    42601 ttttcgttga gaaagttcca ttactgcacc gacaaaatca tttgggacca tgatctgtgc
    42661 tttgacgaat ggttcttcta catcctgaat cgttacaggt tcaggaaaat ctgccgggtt
    42721 atctactgta gcagtcgtac catccgtttt attaacatgg taaataacgg atggtgcagt
    42781 cgtgatcaat tctagattga attcccgttc caagcgttct tggacgacat ccatatgaag
    42841 aagtccgagg aatccacagc ggaacccaaa acctaatgct tgagaggttt ctggttcgaa
    42901 ttgtaaagca gcatcattca attgcagttt ttctaaagct tcccgcaaat cgttgtaacg
    42961 agaagtatca attggataaa gaccacaata aaccatcgga ttcatttttc ggtaacctgg
    43021 taaagcttca gcggcaggat tgtctgctaa cgttactgta tcccctactc gtgtatcttg
    43081 gacagttttg atacttgctg tgatataacc gacatcaccg accatcaaaa agtctctagc
    43141 aactgctttt ggcgagaaga ccccgacctc tgtaacatca aatgttttgc cattactcat
    43201 cagtttgatt ttgtctccgg gtttgaccat accgtccgtg atccggacat ttagaacaac
    43261 accgcggtaa ctatcataaa tagagtcaaa gatcaaagct tttagcggtg catctaagtc
    43321 tccacttggt gcaggaacat attccacgat ctgctcgaga atatcttcaa tcccaatccc
    43381 tgatttggca ctggctaata cagcttcact agcatcgatt ccgattacgt cttcgatttc
    43441 ttgacggaca cgttcgggat ctgctgcggg taggtcgatt ttattgatga ctggcaggat
    43501 ctccaaatca ttgtctaacg ctaaataaac attggccaat gtttgtgctt cgattccttg
    43561 tgctgcatcg accactaaaa cggcaccctc acaggcagca aggcttcgag agacttcata
    43621 ggtgaagtcg acgtgccctg gggtgtcaat caagtgaaag atatatgttt ctccattttt
    43681 cgctgtgtaa ttgagttcta cagcatttaa tttaatcgtg attccacgtt cacgttccaa
    43741 atccatcgag tctaacagct gatcttgcat ttcgcgagac gtcacagtgt gcgtcatttc
    43801 taaaatacga tcggccaatg tagatttccc atggtcgata tgagcgatga tcgaaaaatt
    43861 acgaatctgc tcttggcgtt ctttcatttt ctttatatcc atagcgtctc ctcttttcat
    43921 ataatcagca ctttccatta taccaacgaa aagcagtaat ttaaagagtt tttaattagt
    43981 cagctataga agtttttttt gtagaatttt gctatactgt tatcaagcta ataaatggag
    44041 atgaaaaaat ggatcaaaaa gattttagag aaaatcttga attaaaaccg gtcactcaag
    44101 aacatatcga tcaattcaat gaattgcttt cctatgtgtt ccaagtgact gaagcagaca
    44161 tcgaggaaag cgggtttgag aataaacgag cttttatccg ttccaaaaaa ccgattttgg
    44221 aagtatcgaa agtgttcgga tggttccacg aaaataaact gatctctcaa atcgctatct
    44281 atccttgtga agtcaatatt cacggtgcac gttataaaat gggtggtgtg actggagttg
    44341 gaacttatcc agaatatgct aaccatggat tgatgcaaga tctaattatc gttgcattgg
    44401 ataatatgcg caagaataag caatggatct cttatttgta tccttacagt atcccctact
    44461 atcggcgtaa aggatgggaa atcatgtctg ataaactttc ctttaaaatc agagatacac
    44521 agctacctaa acaaatccca gtaagtggta tggttgagcg attaccagtt gatcatccag
    44581 acgtattttc cgtttatgac aaatttgccc aacaaaacca tggtgcttta ttccgcagtg
    44641 attttcattg ggaagagtat tggcgtttcg aaaatgaaga tgagcgcaca gccgctgtct
    44701 actacgatag caaccatgaa ccaagaggtg ttctgttcta ctgggtagct gaagaaatat
    44761 ttcacgtaaa agaaatgttc tatcttgatc aagaagctcg aaatggctta tggaacttta
    44821 tttccgcaca tttttccatg atctactggg tacatggaga tatttataaa aatgagcctt
    44881 tagcattttt gattgaagat agtcagatca aagagcagat cgaaccatat tttatggctc
    44941 ggatcgttga tgtaaaagaa ttccttcaac ggtttccgtt tgttggaaca gctgatgctt
    45001 ttcatttcat cattgaagat cctgtcgctc cttggaataa tggtgtcttc gctttaacat
    45061 gggatgaaca gggacaagtc cgtgtcttaa atgaacctat aggaaaatcc gttcgactgg
    45121 atatccaaac attgacttgc ttgatgatga attatcgccg tgcttcttat ttggcaagaa
    45181 tcgaacggtt agaaacagat gaagaaacac tgaagtcact agagcgtatc attccaaaca
    45241 tggaagctta ctttagcgat tatttttgat aaaaaggatg atgtttagat gaaaatttat
    45301 tttgccgcac caatgtttgc taaaagtgat ttgttatata accaacagtt agttaaagaa
    45361 atcagagagc tctcttctga gctttcgatc tatcttcctc aagaaaatga agcaatcaat
    45421 gacaaaactg cttatgcaga cagtcgaatg attgctcttg ctgacacaga aaaagtgttg
    45481 gctagtgatt taatgattgc tttactagat gggctgacga tcgatgccgg ggtagcatcg
    45541 gaaatcggtg tggcttatgc gaaaggcatc ccagtgatcg gcttatatac tgatactcga
    45601 cagcaaggcg gaacccaccc gaaaaaaatc gctgcactac aagaaacagc tgaaaatcaa
    45661 tttcactacc tgaacttata tacaataggt ttgatcaaat tgaacggaaa agtggtttct
    45721 tcagaaattg agttgctttc agaagtaaaa agatttttag atggagggac tttcagtgat
    45781 taaagaaatc aaaaaggtgc aaatagcttt acttgctttt ggtgtattcg ttgtctgcta
    45841 taatttttat gaatttatca cacaaaaata ttcgacttct caaggaatca catttatcgt
    45901 ggaatcttta ttagggattg cattgatttt catgccacaa gtcattttga aagttttcaa
    45961 acttaaaata ccagcagcaa tcgttttgtt ttactggttt ttcttgttca tctctgtctt
    46021 tttaggcaca gggatgcatt tgatcagtat catctctttt tgggacaaaa tcttacatgc
    46081 ggtgagtcca atggtcctga cagctcttgg ctatggattg attggttact tgatgaaaga
    46141 tgctgagata tcaaaaacca gtccttggtt gttcctctta ttcggctttg cgtttgctgg
    46201 actttgcggc gtgttttggg agttctggga attcttatgc gaccagttct taggcatgaa
    46261 tcttcaacgt tttgctgctt ctgatggcac actgtttgtc ggacgagcag cattgatgga
    46321 tacgatgggc gacttgctga caaatacgat aggtgctgcc ttaatgggat tgtttgcatg
    46381 gtctcaaagt aaaaaagatg agcgttactt tgaaagttat aaattagaaa aagtgaaaaa
    46441 tacctaataa aagaggttgt gacagaagcg ggcagcttca agaaatttcg gcttattttt
    46501 cgaagaaact acttctggaa cactgtttat ttgaaaaaag gaggtctgac actagttgtg
    46561 tcagacccct tttttctttt atagtctcaa tggaagatta gacgatttct tctagctctt
    46621 ctgttttgaa atgaagttgt ccatcaagta aagtaatcgt tacttttgat ttaggcataa
    46681 catgtcctgc aacgatttct ttagctagtg gtgtttctac ttccttagtg ataaatcgtt
    46741 tcaatggtct tgcaccatat gctggttcat aagcattttc agcaatccac gttttggctt
    46801 catcactgat tgtcagcagg atttcttgat gttccagacg ttgtgccaat tgcgccacca
    46861 ttttgtcaac gattcctttc acgttgtcta ggctcaatgg tgtaaataaa atcgtgtcgt
    46921 cgattcggtt caagaattct ggtttgaagt ttccacgtaa tagcgtgttg acttgttcag
    46981 ccactgcttc tggaatcgtt ccatcagcag tcacgccttc cagcagtaac tgtgaaccga
    47041 tattgctggt catgatcaga accgtatttt tgaaatctac tactcgccct tttgaatcag
    47101 tcaagcgtcc gtcatctagg acttgtaaca agatattgaa gacatcggga tgcgcttttt
    47161 cgatttcatc caacaagacg attgtataag gatttcgtct gacagcttcc gttaattgac
    47221 caccttcttc gtaacctaca tagcctggag gagctccgac tagacgagac acagcatgtt
    47281 tttccatata ttcactcata tcgatccgta ccatatgatc ttctgagtca aacaagttct
    47341 cagctaacgc tttcgctaat tcggtttttc cgacaccagt tggtcctagg aaaaggaaag
    47401 aacctagagg acgatttggg tcttgcaatc cagcccgtga acgaattacc gcgtcactga
    47461 ccgcatcaac tgcttcatct tgaccaatca ctcgtttatg aagtgtttca ttcaatttaa
    47521 ttagtttttc tcgttcacct tctacaagtt tcgttacggg gatacccgtt aatctgccaa
    47581 cgacttgagc aatctcattt tcggtaacgg actcttgaac catcttaatc tcgctgtctt
    47641 ttgctttagc ttctaattcc ttcaattctt tttctaattg aggtatcgta ccatgacgca
    47701 atacagcagc gcgttctaaa tcatagttat tttctgcatc ttctaattcg tgttttgctt
    47761 tatcgatttc agcacgtttt gctgaaacag aattgacttc ttctttttct gtttcccact
    47821 gcatcttcat cgcatttgct tcttcacgaa gttcagctaa ttcttcttgc aaatttttca
    47881 agcgtttttt acttgcatca tctgattctt ttttcagtgc agcttcttcg atttccaact
    47941 gcatcaaacg tctagtaact tgatctaatt ccgttggcat agagttcatt tctacccgaa
    48001 tcgttgcact ggcttcatcg atcaaatcga ttgctttgtc cggtaagaac cgatcagtaa
    48061 tataacggtc agataaagta gctgctgcta ctaatgcgtt gtcatggatg ttaacaccat
    48121 ggtggatctc aaaacgttct tttaatccac gcagaatact gatcgtatct tccactgttg
    48181 gttctttgac taataccttt tgaaagcgac gttccaacgc tttgtctttt tccatatatt
    48241 gacggtactc atctaaagtt gtggcaccga tcaaatgtaa ttcaccgcga gcaagcattg
    48301 gtttcaacag atttccggca tccatacttc cttctgtttt tcctgcccct acgatattgt
    48361 ggatttcatc gatgaacaag aggatacgtc catcactttt tttgacttct ttcaagactg
    48421 cttttagtct ttcttcaaat tcaccacgga attttgctcc agcgatcaat gcacccatat
    48481 ctaatgaaaa aatcgtttta tcttttaaat tttcaggcac atctttgcgt acgatacgtt
    48541 gagcaagccc ttcaacgatt gccgttttcc ctacaccggg ttctcctatc agaacaggat
    48601 tattttttgt ttttcgtgaa agaatacgga tgacatcacg gatttcttca tcacgaccga
    48661 tgattggatc catttttccg cttttgactt gctgtactaa atcaacgccg tatttttcta
    48721 atgctttgta ttgttcttct tggttttgtg aggtcactct ttctcctcct ctcatctctt
    48781 cgatgttttt acgcaattct ttttcagata atccgttttt attcaaataa acagttaacg
    48841 gataattttt taatttcatc aacgctaaaa taacgacttc cgttgaaaga aactcgtcgc
    48901 cgaagctttc acgtatttgg tctgcttcat tcaataatcg aaagaaattc tggctgaggt
    48961 tttgtccata ttggacgttt ccaccagaaa caactggata ctcatccagt agacgatcaa
    49021 tttcatgttc aaaagattcg acatccaatc cggcatccgt atagaaattt cgaccaaaat
    49081 gattagattg taaaaagatt ttccatacat gggcaatgtc gatatcttga tgttttcgag
    49141 tgaccgcaat ctgttgagct tcagcaatcg cttcttgaag cgtcgtagtc attttctcaa
    49201 tattcataca ggtataccct cctaattaaa ggtaagatca cttatttctt tgatctttac
    49261 aaaaactatt atatcgcttt ggtcaatttt ggtcaaaata tacacatagt tttttgactt
    49321 tttttctcga cttatataaa tcttaaaaaa acaaaattga ttgtatgtat ataaaaaaag
    49381 agccaaacaa acgtaaaatt atcttatcca taacgttttt ctttgatttg ataaaaaggg
    49441 tagatcttat aaaagaccta cccttgattt tctttattct ctttttttac ttctattatt
    49501 tcttctttga tcactcttcg ccgttcagca atcacataca caaatccata gagaaatggc
    49561 aagataaagg aactgaaacg atacagtaaa agcgaagaaa ctgcgtcaac tgttccaaca
    49621 actggtctga ataataacaa atagacaaat tcaaaggaac caataccagc aggcgtagga
    49681 ataacccctg aaagcactac tgcaaagctg ataaacgaaa tggtcagtag aaaatctatt
    49741 ttaggatggt tttcaatcag aatcaaataa ggaagtacat accaaaatac aagttttaac
    49801 atattccata gataaatccg caatagcgca gtcctgtctt ggataatggt ttggaccgtt
    49861 tcccttaaag agtagatctg ctgattgcat gtatccaccc atttccgcaa acgctcactt
    49921 ttaaataact tattcgataa gctgacaaaa aatatttgaa cattcaagct gacggacatc
    49981 gccaataaaa aggcaacaat cacgatcgtc aaaaggatac cagcgatgat aaaccagatc
    50041 attgatggac cattagaata aaaaagagag aattggatga ccaagccagc taatgcataa
    50101 gtgatcactg ccagtttgta cataatcata tggagtgctg tcacccctgc tccctgggat
    50161 agcttcattc cttttttgtt ataaaaataa atctccgaga tcagcgttcc tgtaccaaac
    50221 gaaacgattc ggtaaaacgc aatatagcta gaggtcaaga acccatcttt cgttgtaaaa
    50281 ttcggcgcga aaggttcagc aatttctttg attgagcgcc cttcagcgat ttggtaaact
    50341 gtccctaata taacagccgc tgccaaaaca acaacactgg tagacagcaa ttcggcaaag
    50401 atatctgcaa atgattgatt gattaaataa taaataatgg caaaaatcac aatcagcaaa
    50461 agcagattca gaatgatttt agttttcgtt cctttagacg acatctcctg ctccttaatg
    50521 gaaaaaataa atgagaatca aatcaacaag tacacctgca gcgaatccta tagtaaactt
    50581 ctttttccga gttttatgat ggaaaacttt tcgagagatc aatcctccca aaccaccgcc
    50641 taacaggctg acaaatatca aattattctc aggaacacgc cagccgctag ttctagcctg
    50701 atgtttatca taccccatca gacaaaataa gtaaaaattc acaatgaaaa ggtaaaacca
    50761 caaaggattg tgtaacaata catccattac tcttcttcca actttctgtt aactactatt
    50821 tctaagcttt ttcttctttc tttggtgaat aaactgttaa tccttttgat acaacagata
    50881 attcaactgg aagatcatct gtcgtgtctc catccgttcg agtgcccaca gatttttcct
    50941 gcgcttggat tttcatgcgt ttggctgtga aataagtaat gccggggatc gtatagaatt
    51001 tgcctctcat gattctaggc aaataaaaaa taaatcggaa aatatctaag gaaggtacga
    51061 tcgttacatg aaattttcca tcatctaggg tcgctgagtc gtcaaaattt gtaaaaccgc
    51121 ccacggagtt agacatcgtg actgtcaaca gttgggattt tcctttccaa tgctcctcgt
    51181 ctgtttctaa atccaaatga tattttttct tcttcagcaa taacttaaaa aacttcttga
    51241 taaagatcca cgagccgtaa cgctgttttt cttcttgact gatccaaact gctgtatctg
    51301 ctaagattcc gattgtcagt gtagagataa tgacttcatc attgacttga ccatagtcta
    51361 ttttccgtac attgccttcc aggatcacgt cagcagcttc ttcttttttg aggggaatct
    51421 caagaacacg tgcaaggttg ttgacagttc cacctggaag taatccgaca tggtattgag
    51481 ataatgtttt ttggaacgct tggacgatat gatgaatcgt gccgtctcca cctataatta
    51541 ctaaagtatc agcttctatg gcttctgctt tcttgactaa ttccgcatca tcaatcgatg
    51601 ggccagtttc agctagttct acatataatt ctggctgctt ttcttttaca taatcttgaa
    51661 accagcgtgc taatttctca ccctcatctt tccctgaact tttgttataa aaaactagca
    51721 gcttttccat tctttacctc ctatttactc ttcaaaaaag agggcgggac aaccaattcc
    51781 attgtcctgc cctttagcct tagacagatg acattccatc attgggacga acgccttgtc
    51841 ataagctgtt ctttttcgat tttaactctt tgtgttcaat tctgcaattt ttacaatcac
    51901 gtctgttgct ttttccatta cttgtaaaga aacaaattcg aatcgtccgt gcatattttc
    51961 tcctccggca aatagatttg gcgtcggaat acccatgtaa gagatttttg aaccatctgt
    52021 tcctccacga actggttcga tcactggaga gataccaagc tcgatcattg cttgtttcgc
    52081 taattccacg atgctcatat ctttttcaat gatttctttc atgttgtaat attgatcata
    52141 catatctact ttgattcgtt cttgatcaaa tcgctgattc aactcttctt ggatatgtgt
    52201 gatttgtgct ttgcgtgctt cgaatttttc acgatcatga tcgcgaatga tataactcat
    52261 agaagcttct tcaggattcc catccaatgc catcaaatgg aagaaacctt cgtatccttc
    52321 cgttttttct ggaacttcat cggctggaag ctgattatga aaatcgattg ctaactgtag
    52381 agcattgatc attgtatctt ttgccgtccc tggatgtaca tttttccctt ggatcgtaat
    52441 atttgcttga gcagcactaa acgtttcata ctgcaactca cctaaaggac caccatcgac
    52501 tgtataagca aaatctacat cgaattgctc aacatcgaat ttatccgctc cgacaccgat
    52561 ttcttcatca ggaccaaaag ccactttgat ggtaccgtga gggatagaag gattattgat
    52621 caagatctcc attgcagtca tgatttccgc aatccctgat ttgtcatctg ctcccagtaa
    52681 tgtactgcca tctgttgtga tcaatgtgtg gccttcataa tttttcaaat ttggaaaatc
    52741 ttttgtattc aatgtatagc gttcttccgc atctagtacg atcgtagact cgccatcata
    52801 gttttcaatg atttgtggat tgactcctac tgcattaaaa tctgctgtat ccatgtggga
    52861 aataaatcca atggaacgca cttcatgatc aacattgctt ggtaatgttc ccataacgaa
    52921 tccgttatct tcattataga aaacatcagc taagcctaac tcttctaatt cttttttcaa
    52981 ttcttgcgca aaagcaactt gtgtctgagt cgatggagtc gttttgcttg ttgcatcgga
    53041 gcgtgtttct gttttgacat atcgtaaaaa acgtggcaaa agattttcgt acatagtatt
    53101 ctctcctttt aattaaattg aaatgggttt gtatttgttt cagaaacgaa aaagttaata
    53161 tcccaatttt cttcttgttt ccaagattca aatttctcga taaatcgttg cttgcataac
    53221 gattcaatat aatgaccggg atcaattgca atcaacccag cgctttgcat atcatgagcc
    53281 gtatgataat agatatctcc cgtgatatag acatctgctc tttgggcgat tgcttgcgga
    53341 taaaattttt caccgcttcc accgcaaatc gcaatgcgct tcacagagga ttttgcattt
    53401 tttggctgga ctattctcag tccatctagt tggaacgctt cttttacttg ttctacaaat
    53461 gcctctatag aaatttcttg cggcaattca cctactcgtc ctaacccaaa catttcgact
    53521 ggttcatcta tagcgaaaag atcataagca ggttcctcat aaggatgagc tgaccgcatg
    53581 gcttgaatca cttgtttttc gatagtttcc ggcaaaataa cctcaacttt tgcctcttga
    53641 acttgttctg tcttccctac tttcccaatc gctggttgtg caccagcttc cggagtaaat
    53701 cgaccgtgtc caatcgaggt aaaacttgta ccggtataat caccttgtgt accagcacca
    53761 gctgcagcta atacttcacg cattttctgt gcatgatcca caggtacata aacggccagt
    53821 tttttataat gaatctcatg agtctttact aaataactct ccacttcaat accgagaagt
    53881 tcacaaaacc agtcattcag tccatcccaa ataatatcca tgttcgtgtg tgctgcatag
    53941 acggcaatat catgtttcaa caaatctgca tacatttttt cttgtggctg atcagtaacc
    54001 agccgtttta caggacgaaa gattggagga tgtttagcaa tcagcaaatc gatttttttc
    54061 ttgattgctt cttcgaccac ttctggacga acgtctaatg tcatcattac tcgttggatt
    54121 ggcttatcca atgttccgat atgcagtccg actgggtctc cttcttcagc tagccactga
    54181 ggacaatagc tttcaaactt ttggatgaaa gtgcgtccgc tgattgtcat ggctttaaaa
    54241 cctcctcaat catttggatt tcctgctcga tttgtttttg acgtgcctct tgattagctg
    54301 cttgtttgag ctgttcacgg atagcttttc tttgttgaag ctctcttgtc cattttgatt
    54361 tgaagatctc acttttctct tcaagcagaa atggcccgaa catcagctcc atttcattgt
    54421 aagtaactgg tttttgttct ttttcggcta caatgatttc atatattttt ttattttctt
    54481 caagtatttt ttctgattgg attttatact gattttcttg cagccaagat cgtaatgtac
    54541 gctctccgat gtttggctga aggatcaaac gttcttgtcc gcttaagcgt tgattcaact
    54601 tgccagcttc taggatatct cggatcagtg aacctcccat acccgcaatc gtgacagctg
    54661 taataccatc ttgtggctgg atagcatcta gaccatttgc aaggcgtacg gtgatacggt
    54721 cttctaatcc atcttttttg acttgcttac gtgctgattc ataaggacct tgaacgactt
    54781 caccagcaac tgcatattcg atttttcctc ttaatatcaa tgcaacgggt aagtaagcgt
    54841 gatctgatcc aatgtctgct aaacgagcgg attcagggac aaagtcaccc actgcggcta
    54901 agcgctgaga taaatctaaa tggttcatag cttcactcct tctagataat tatagcattt
    54961 tccaaataaa ataaggagac taggaataaa aaaattccca tatctcctta aaaaatatac
    55021 cttttcgttc tataaaacaa cattttctta tttttgaaaa aatgttattg gttatttgtt
    55081 caatgaaaca tcaataatcg aatctccagg aacttgcggt gggatagaca tcgtcatgat
    55141 cggcatctca actaatgtaa attggacttt gtcaccgatt ttcaattcat ttatgtccac
    55201 gcttattttt tcagcagtag catttaagat cactccgtca ttgctgaatg acgttcccat
    55261 attcgctgga tcttctttgg ccatgacatc gacgagtttc aactgaagcg tttcttcttc
    55321 tgcttctact tgagtgatac tttcaatcgt tccgacaaat atgctttgtc tctccgtttt
    55381 cgtactgctt gtttttactg tttgacccgc atcaatcgaa tcaacagaac ctgtgttgct
    55441 atctgcgcta ccgcagcctg ctaaaagaaa aaatagacca actacagata tactagaaaa
    55501 aatagccctc ataaaaaatc ctccgtttaa gtttctgcat tacttgttgc ggtgattgtc
    55561 tccggtcatt tctgtccata taatgattcg ctgacctgct tttactacta ggtcaaatgt
    55621 cagttggact tgtcaactgt tcaaccattg cgtcggttgc gaccagtcat ttcgatttct
    55681 tttgtcaaat aacgtatgcg ttccattatc cgtttcgctt ttagcggctc ttcatctcca
    55741 cgttgagtga cggacaaatg ctgttccaat tcatccattg taaaattcga tgagaagaag
    55801 gttggtaact gttcctgcat ccgatattga aggataacac cgaatacttc gtctcgtatc
    55861 caactactca tcgcatctgc accaatgtca tcgatcatca aaataggtga acgtttcact
    55921 gcatctaact tttctgccac ttgatctttt ccgattgcct gtttcatctc aacggcaaat
    55981 gtcggaaaat ggactagcgt cgtggtgaat ccagcaatcg ctaattctct cgctgttgct
    56041 cctaataaat atgtttttcc aataccaaaa cttcctacta gatacagtcc tttatgatac
    56101 ccttttggat cagcagtata tttctctaca aaatctaggg cttctaagct cgcatatcct
    56161 cgtccatctg tacgttcaaa gttttcaaaa gaagccgatt gaatatcttt tggcatatcc
    56221 attgctttta ctcggcttct gatttcttct tgtctttgcc gtgctaacaa tgcttccgtt
    56281 ggtacataag tgacatccac ataatgaaaa ttcaacgtca gcttcggctc atagccggga
    56341 gcgatcatcg ttggatcgtt tttttgatat ttacgctttt cttgcacgaa ttcatacaat
    56401 ttagcgtaac ttttttcaat atccgcttgt gtcaattctt cttggtgtgc gcttagaaaa
    56461 gcttgaacat ccgcatcatt taagacttca gcgatcattt cagcatactt ttcttgataa
    56521 ttgcgtttgc tgatgatcct cttcaattct ttgcctacgt cttccactag atatcaccct
    56581 ccttttttgc caggtattct ttaaaacggc ggtcaatctc tgcttttttc tcgctgctga
    56641 tctgtttttc atcttttggc tggttgaccc aatcaggaag cttttcccgt cgaacatttc
    56701 gcttgccagt ttgataattt gtttgcttct tttgttttga ttgttttgct tgattactta
    56761 tttgccgaat gtgctggatc gcttcttctg ctgtggcgat tttcttttgt gcccattcat
    56821 ttgccactgt atttacataa ctggcattta agctagcatt attcttgatc actagtacat
    56881 aattcagcaa aacattgatc acgctactgg aaagacctga ttgagaaaca agatcttgca
    56941 gtaaccagtt ttcttgttta gaaacatatc cgcctttttc tgacttgatt gcttccagat
    57001 atttcatcgg tggaatgctc tgtgcttctt ttatcagctg aagcgctgcg tttgtcaaat
    57061 gttcgtttga cgagggttca ttactgtttt cagaaaccgc aactgtttct tttgtttgct
    57121 tcaccggacg tctctgcatt gccaaaatgg ctttttgcag ttctttttca gatacttttt
    57181 gttcactaaa gtcataagat tgactgatca gttcgccaag tgtcaattca tcgaaaccgt
    57241 aaagctgatg atacatgtcg acttgtttga tgattggatc atcaagactt gcaatatgtt
    57301 tttttgccag ccaatcattc aaaaactgcc aatcgaatgt cgaatctgct tcaagtaatg
    57361 ctactgattc agattcttca aaagatttat tcagcgattc gatgttttct tgattagcag
    57421 cgtacactgc ttcattgaag cgataaacat ctaaaaattt ttttgtgatt ttttgatatc
    57481 cctctgctgt atgcctttta ggttcaaaga attgggtaag cttctggaat cgtcgttcac
    57541 caactttttc cagaagaaga aaagccagaa cagagtcttt gaaaaatgct ttaggaatga
    57601 gtggttctaa cagctcataa ataaaacgag gtcccagatc actatcttct ttaaaataaa
    57661 catccagtaa gccgattcct tctagctttt ttcttgcatc ataaaattgc gggataccgc
    57721 agcctagact actaagcaga tctgcatgca tcccttcagg acctttgcca gattcttgag
    57781 agatttctga taacagggtg aaatagatac tcaaggcttc tccgccgatt agtggctgat
    57841 aaagatacag caaggcgttc ttgctgtctt ctctcagcgg agccgctttt cttccttggt
    57901 aaatattttt tggttgaagc tctttccaag cattttccaa ggaaatgcct cctttttgtt
    57961 tcttaattgt tttctttatt attctcttta ttattttctt ttgctttatc cacgatatct
    58021 tgcaactctt ttaagaaaac agacatatct ttgaactggc gataaacact tgcaaaacga
    58081 atataagcga tttcgtctaa atcaactaaa tcttccatca catattcacc aattagattc
    58141 gtcgatactt cgttttctcc gagactgcgt acgcggtttt cgacatggtt gacgatttgt
    58201 tccatttgtt ccatagctac aggtcttttt tctgctgaac ggatgagtcc tcttaaaatt
    58261 ttatcgcgat tgaactcttc gcgttccccg tttttcttga ttactaataa cggagcagct
    58321 tcaattcgtt caaatgtagt gaatcggaac gaacaattct cacattcgcg acggcgacgg
    58381 atcgcacgtc catcatctgc ttgacggctg tcgattactc gtgaattatt atgttgacat
    58441 ctgggacatc tcattttgtc tcacctcttg catcgcattt tatataaagg atgtgtcttt
    58501 ctttacaact agttgtatta taacacgaaa caaatcacaa acgcgactgt cctacccaaa
    58561 aaacacggat ataagaacaa ttttttctac tttcatgcgt aattgtgtcc cctgtcctta
    58621 ctaaatttca attttccaat actgatcacc ttgattactg agaaataact tacacaaaag
    58681 ctgatttagt catctagcac aaaaaacttg aagcagatga acaccttctc atctgcttca
    58741 agtcacatct cgatttttta ctattttata acttctcttc gattcctgta tagtgtttca
    58801 ttcgctgttc ctcgattttt tgctcatcgg gaatgtggtc actttctggg agcggtgttt
    58861 tactacgatc aggtgtcgga ttttttcgtt cttgagtcaa atcttgccgc ttcgcttctt
    58921 cttctgcctg ctggtcttct atttcatcga ttcttgcttg gtactgcttt tctttttctt
    58981 ctgctgtttc tgattgcgta cgaccttcat cgattagatt ttctttattg agcatctcgc
    59041 cgattttttc ttttgcgtta cctacgactt tatccttttg attttttaat gatcccatcg
    59101 ctatccctca ctttctatat actcaggata gcaaaattga cacagatact caaagaaagc
    59161 gattagcaaa aagccaattt tcgatttgtc ccacaagttt ttcttttgtt ccattgttgt
    59221 cgaacaaaat atccgcacgc tgcttttttt cttcgattgg aagttgacta ctaattctct
    59281 gctgcgcttg ttctagcgtc agttgattac gtttcatcaa acgctgaagc tgagtttctg
    59341 gcgtagtata aacgacagct actgcatcca tatagtggtc ataatgacct tcgtagagca
    59401 atgggatatc tacaattact aaaggatgat tggaacttag agaggcgatt tgatctttta
    59461 tcgcttttcg gataaacgga tctaatagct gatcaagttt ttgccgtttt tcctttgatg
    59521 gaaaaacaat catcccaagt ttccctcgat ctagttgccc atcaggctgc aatacctctt
    59581 ggccaaacac tgatacaatt ttcctcagag caagcgttcc aggctcaaca gtttctctgg
    59641 caatcacatc gccatcgatc aaaggaaatc catgagactg aaagactttg gctgccgtac
    59701 ttttaccggt agctattcca cccgtcaaac ctaaaacaaa tcctactttg cgatttttag
    59761 cctttgacaa ttcggacaat agtgcgtccc cctttgtgcc acttttgttt tcacgatcgg
    59821 cgttccacaa cgtatacatg gtttgccagt ttgctgatag acatgcagtg ctacttgaaa
    59881 tccgcctgat tctcctaatg cattcaaata actgcgaatc gtggtacctc cggcttctat
    59941 tgcacgatcc aaaacatcaa tgatcgatag acgtaattgt tctacctctt tggggcgcag
    60001 tgtgttcgcc ggttgttctg gatggatctt cgcttcccat aatgcttcat ctacataaat
    60061 attacctaga ccagtcacta gacgttggtc tagcagcaat ggcttgattg ctttgtggga
    60121 ctttttcagt ccatttgcga aatctgctaa taagaagcta tctggcaaag gctcaggtcc
    60181 caatttcatg attcctcggt aagttgcact tgctcctttt tcgacgattg tcatacgacc
    60241 aaattttcgt acatcgttgt agcgaagttg gcttccatcc tcaaaaaaga aaagtacatg
    60301 cgtatgttta tcgattggtg tattttcttt cgtaaattga tattttcctt ccattcgcaa
    60361 gtgtgagatc agttcacaat gatcgagatg aaaaattaaa aacttccctc tacgtccaat
    60421 ggattggatg gtttcgccaa caagagaagc ctcaaagatg ggtgtctctg gttgctcgat
    60481 gatcttgggc cacaagacct gaactttttg aatcgttttt cctactacta accgttccaa
    60541 tcctttacga acggtttcta cttctggtaa ttctggcatt ttttcttttc accttcatta
    60601 tattgctctg tcttaaacaa tgtttatttt gcttcgtacc acgtttttcc ccaactgctg
    60661 tcagtgatca gtggtacatg aagagaaaca gcttgttcca taacttcctt caccaattta
    60721 tctaatgatt caagttcttt ttccggtacc tcgaagacaa gttcatcatg aacttgtaac
    60781 agcatcgtag cttgaagacc tgtttctttt aatcgtttat ccaattcgat catcgcgatt
    60841 ttcaaaatat cagcagcact tccttggatt ggtgtattga tagctgttcg ttccgcaaaa
    60901 gaacggatat tgtaatttct tgaattgatg tctggcagat atcgacggcg gtgatacagc
    60961 gtttcgacgt agccttggtc ttttgcttcg cgtacgattc tttccatata ttctttgaca
    61021 cctgggtatt tttcaaagta cgtatcgatg tattgctgtg ctgcttttcg gctgattcct
    61081 agattttgtg aaagtccgta atcagagatg ccatacacga taccaaaatt cactgctttt
    61141 gcctgcctac gcatatttgg tgtgacatcc tctgcctttt cgattccaaa tacacgcatc
    61201 gctgtgctgg catggatatc ttgaccttct aggaaggcag ctttcaaatg ttcatcattt
    61261 gagatatgcg caagtacgcg taactcgatt tgtgaatagt cagaagaata aatcagccag
    61321 tctttttctc taggaacaaa tgcctgacgt atctttctcc cttcatctaa tcgaatcggg
    61381 atgttctgca aattaggatc aacagagctt aatcgtcctg tttgggtcaa tgtctggaca
    61441 taacgggtat gcacttttcc atctgattgg atcactttca gcagtccctc gacataggta
    61501 gattgaattt ttgcaatttg acgataggtg aggatatcct ctacgattgg tgcttgttca
    61561 cgaagctgtt ccagtacatc tacggctgtg gaatatccag tttttgtttt tttgataacg
    61621 ggtaatccca ttttttcaaa taagatcact cctaattgct ttggtgaatt caaattgaat
    61681 tcttcgccgg cttcttcgta gattttttgt tcgatctctc ttaagcgagc agagaactcg
    61741 cccttcattt cttgtaagcg agtcgcatct actttgatcc cgcttatttc catctctgcc
    61801 aaaatgatag acaatggcag ttccattttc cggaaaagat cttcttggtt tttttctgcc
    61861 agttcttggc tcaattttgg tgtcaacgca ttgattgcag ctacttttcg cgctaaatga
    61921 gcaaaaaaga gttcctcttc ttctggtaaa ccttttttcg cccctttgcc gtatacagct
    61981 tcatctgatt ggatatcgtt gtatccgtaa tgagcagcta ttccttcgat atctgtgctt
    62041 ttttcattcg tatcgagaag gtatgcacct agcaagacat caaaatcgat tcctttcgtt
    62101 tttccagtat agcggtttaa agcaacataa gttcgtttcg catcatatac ttttttcagc
    62161 cgagtcggat cagtgatcca agaatggaac gctcggtttt cgaatacaga tagatcgttc
    62221 gtgacataaa ttttcttttc tgtcccccac gctactccga cgatttcctc tgtatgataa
    62281 ttgtcgccca tcatttccac gtacaacgcc atatccgtag taaacatttc ctctttgaat
    62341 tcatgaacga cttcaaacaa aatatcatcc atttttactt cttcttctac gatattcaat
    62401 ttagaaagga actgattgaa atccatttct ttataaaacg gaacgagttt atcgagattt
    62461 ttcccttcat acttcaatga gtcgatagag acatccacag gcgctgaagt attgatggtc
    62521 gccaattttt tcgataagaa agcctgctct ttgtcattta tcagattttc tttcattttg
    62581 ctttgcttca tttcatcgat atgttcataa attccttcga ctgagccata ttgtttcaat
    62641 agcttgatag ctgttttttc accgatttta gtcacaccgg ggatattgtc agaggcatct
    62701 ccggctagtc ccttcatgtc aataatctgt tttggcgtca acccatcata tttttccgct
    62761 acatgttctg gtgtatagct ttcgatatcg ctgacacctt ttacagtgat atccactttt
    62821 acttctttac tagctaattg agtcaaatcc cgatcacctg aaagcacgac gacatcaaat
    62881 tgatctttgt ctactttttc tgccaaagta ccgataatat catctgcttc ataattgggc
    62941 aattcgtaat actgtacccc tagtcctgtc aacaaatcac gtatatatgg catttgttct
    63001 ttgaattcgc caggtgtttt ggatcttcca cctttgtact ccgcatacat ttctgtccga
    63061 aaagtcgttt ttcctgcatc gaacgcaacc agaacatgag tcggttgctc tttttgcata
    63121 acattttcaa acatcgtatt gaatgcatag attgcatttg tatgaagacc gtttctattt
    63181 ttgaaacgtt caagagaatt gtgaagcgca aagaatgcgc gaaaagccac actgttccca
    63241 tcaacgagta ataatttatt ttttgtcatc ttcttaactc cttactgtaa agttcttatt
    63301 cattctaaca aagaatcctg cggaatgcga aaagaaaaag cgggaccata ccaaataatt
    63361 cggtatagcc ccaactgatg aatttttttc ttttttagtt gttccgtcca aagattctca
    63421 gaaacgccaa gaacaagttg ataaagtcca aatacagctt caatgccatg aatacagcaa
    63481 ttccactatg tgctgttccg tttgtctgga catagatatt acggatgctt tggttatcat
    63541 aagctgtaat ccctgcaaag atgacgacta acagtaggga gatgaacagc tcaactacgc
    63601 tacttctcaa aatgaagatg ttcaagacta gtgcaatgat cactccgatt aaagctgcct
    63661 gtcctgctct tccaaccccg ctaagatctc tttttgttgt tactccgaca acagacatcc
    63721 ccatgaacat caaagtagct gtcaagaaag cttgcgttac tgttccaata tcgtagtatg
    63781 caagagtaac cgctaatgtt atgccattca aaagagagta ggcaataaat ccaccgattg
    63841 ctaagctcgg attcttttgt gcttttacgc ctagaaccat tactaaaatc agttcaacga
    63901 tccacagacc cacaaagcct agtggaaaat tattgataaa agttgctaat tgcaaaggaa
    63961 atacccaaag agccaagtaa gaagtgactg cactaagggc aagtcccata cctaaaaatc
    64021 cataaacttt tgcataaaaa cgattgatac ctgacattga tgtacttgga ttattcattc
    64081 aattcacctt cactttcgat tgctatttcc aattcaggaa cttgtggcgg ttggaccata
    64141 acgacagcat ccaacacacg atcttcgttc gtattctccg cttctacaga aatcgtcacc
    64201 acatccttca tcttatctac ttttatgacc tcaaactgaa aagtcagtgt ttcataatgg
    64261 aaaaccggtt caataaaatt gacagaaaaa ttgacaacat gtgatcctgg tccaggaaga
    64321 tgctttgaaa ttgcacttgt gatgatcccc atcagcatca ctgatggaac gattggctgt
    64381 tgatattctg ttttttgtgc aaagtcatgt tggatataca aaggatttgc atcattcgtc
    64441 aatcccaaat acagcaaaag ctggttatct tcaatagatt ctgttaagga taacgagtcg
    64501 ccttcctcga tttcatttat tgtttttcct agttttctcg gcttacctat gttcaagata
    64561 gaaaccctcc agtttatttt taatcataca ttattgtacc aaaagtagcg taaaaggcaa
    64621 gttctgctcc cttgatttaa cagattccta tagtttacag aggtcgtgaa aaagctgtcc
    64681 tgcatcaaat aataagaaca gccaagaaaa aacagcttct catgtttttg atttgattgg
    64741 gcttattacc gcagatgcaa gcttttgaac accgtttatc ataagttgca aaaaaggggg
    64801 cttggcaaga tttttgccac tccccctctt tctattttta taaggacact gcagtaattg
    64861 tattacagta catttttcgt cgtgctgctt aaaagttttt catacgcttg ttcaaatttt
    64921 tgaacatctc cagcacccat gaagattact actgcatcat gataatctaa caatggagat
    64981 acattatttt ctttgatgac ttctccacct tttttgattt tcgctccaag gtcttcaatc
    65041 ttaacgtttc cttgttcttc ccgagccgaa ccaaaaatat cgcataagta aactttatca
    65101 gctaaatcaa gtgcttctgc aaattcatcc atcaatgcaa tcgtacgcgt aaacgtatgg
    65161 ggttggaaaa ctgcaattat ttctttatct ggatattttt ggcgtgcgcc atcaatcgtt
    65221 gctttgatct ctgcagggtg atgcgcataa tcatcgacta cggtcatgtc tgctacgatc
    65281 ttttcactga atcgacgttt tactcctggg aaagtcagca tttcttctgc aacttctttc
    65341 agatccagtt tttcgaaata agctactgca ataacaccta aagcatttaa gatattgtgt
    65401 ttaccgaatg ctggtacagt gaagtgccca acaaattcat ccccatgata gacatcaaaa
    65461 gcagaaccag aagttgtgcg ttcgatgttt cgtgcttgga tatcatcgtt ttctgtgaca
    65521 ccataataat aaatcggcac atttgctttt aatttgcgta aataagcatc gtcaccatac
    65581 gcaaaaatag cttttttcac ttgtccagcc attgtttgga aagctgtaaa tacatcgtcg
    65641 atacttgtat aataatccgg atgatcaaaa tcgatattgg tcatgattgc atagtctggt
    65701 gaataggcaa ggaaatgacg gcgatattca caggcctcaa atgaaaagaa ttccgcttgc
    65761 ggatcaccat gtcctgttcc atctccaatt agataacttg ttggacggac accactcaat
    65821 acatgagaga gcagtccagt cgtacttgtt tttccgtgag agcctgttac agcgatactc
    65881 gtataatttt ggataaaatg accaataaag tcgtgatagc gaatcacttc caaacctaat
    65941 tcttttgctc gctggatttc ttcatgtgaa tcaggaaatg catttcctgc aatgatcgtc
    66001 atccctggtt ttacattatc tgcgttaaat ggcagaatcg tgatatttgc tttttctaaa
    66061 tctctttgtg taaagaaata tttttcaatg tctgatcctt ggacatttag accttgttca
    66121 tgtaaaacaa gagccaagga gctcattcct gaacctttga taccaacaaa atgatacaat
    66181 ttattttgat tttccatttg aatcctcttc tcttatctag cgtccattct tttagctatt
    66241 acattcaaca cgctcctcat tatcttataa acgattgaaa atttcaacca aatttatttg
    66301 gaaaggagga ttcgcttgct aaaatggtca agtacgagaa aaatccgttt ttattgactc
    66361 tttaattctg gtgtaagaag accaaacttc ctctgatata tcaatgtgag gaataaaatc
    66421 ttttcctttc ggaccatagc cgttctcatc gtcatggaga cgttccattt ctcctaatac
    66481 tagcgcatgg aaatattcct taggccattg atgattcagt tcttttgctg taaagatgat
    66541 ctcttcagaa aagtgaagga tttccggcac aaatggcaca gcattcgcga aaatagtttg
    66601 ttcattttgc catactttct caaaagttgc tcgagtcctg cgttgcagtg tcggatcatt
    66661 catcaataag attttttctg gtacagctcc taaggaatga agaatctcca gtgaaaaagt
    66721 ggcgttctct ccagaatttg tagacttcga ttctatcaga aaagccttat cgggcaaatc
    66781 atagacttct tttagatact gacgacagat ttcactttca ctcatgccat cttcaaaatg
    66841 aaatccttgc ttttcaaaat tctcataaag gattctagtc gcatgcccca cccctccgac
    66901 caaaaacaat tgatctactt ggccatttaa atacatttgt gctgctttat cagctaagat
    66961 aggaagacag tttccagcta aaataagtgt aggtgccgtc agttcctcaa aatttccctc
    67021 ttcaggtgcg gctaaaaaat ttaatagctg attccagtct tttagattcc tctgctcact
    67081 tttttccaaa ggccttctat cctttcatct gattttttgt gcttcattaa agtcttctct
    67141 ttttgtctca atccatttga tttctccatg acatcttcca caacgatatt tgcgtacatc
    67201 gattcttctc ttgcggcaga tcgtggcaga acaatcttgg catcgataat actccatctt
    67261 tatctgtttc ttcgtcaagg ctggtgcata gcgcaaccct cctgtttttt tcaaaagata
    67321 cttgaaatcc atatctttat gctgataccc tcttccagct agatgaagat gataatggca
    67381 aagttcatgt ttgatgattc ctgcaatcgt ctcttcgtct gactcttcaa atagcttcgc
    67441 attaaaatct aaatgatgat cgtttaaatg atagcgcccg ccagttgtac gtaagcgtgg
    67501 attgaacatt gcttggtgct tgaaaggaag ctggaagctt tttaatgata tctcttctac
    67561 taactgctgc agttcttctt gtgtcaattt ttgttactcc tcattgttac tcttcataag
    67621 gggaaagcat cgtcaaactg attctgcctt ttttcgtatc gacttgttct atccaaactg
    67681 ttacgatatc tcctactgac acgacatctg taggatgctt gacgaatttt ttgctgagtt
    67741 tcgatatatg gacaagtccg tcttgcttca caccgatatc aacaaatgcg ccgaagtcaa
    67801 tgacattacg gaccgttcca gtcaattcca tccctggttt caagtcttcc atactcaata
    67861 catctgtacg taataagggc gctggcatct catcacgcat atctcgtcca ggttgtgtca
    67921 gcccagctaa aatatcggct aacgtttctt ctccgactga taggctttca gccagtttct
    67981 ctacggataa tcgtgtcaat ttttctactg cttcttctgt accaagctct ttttctgaaa
    68041 gttggactga agttaatatt tcttttgcaa tagaataact ttcgggatgg atacctgtgt
    68101 tgtccaaaat gtttttccca ccagggactc gcaagaatcc gattgcttgt tcatatgctt
    68161 ttggtcctaa gcgtggcact tttttcaatt gagttcgtgc ggtgaattct ccgttttctt
    68221 cacgataaat gacaatgttt tgggcagttg ttttattcaa tcctgatata tgctgcaaga
    68281 gctgcgggct tgctgtattc acatccacac ctacttggtt aaccgctgtt tccacaacaa
    68341 aatccaattg ttcagccaaa cgtttttgag aaacgtcatg ttggtactgg cctactccga
    68401 cagctttagg atcgattttg actaattcag ctaaaggatc ttgcaggcga cgggcaatag
    68461 aaacagcact tctttcttca acttgtagat gcggaaattc ttttcgtgca atttcgctag
    68521 cggagtaaac agaagcgcct gcttcgttga cgatagcgta ataggtttta tggtcggcgg
    68581 atttcaactg ctcagccaca aaaagttcag attcccgact ggctgtacca tttccgatag
    68641 ctaccatatc tacttgatat ttattaatta attggataaa agctggtcca gcagcttctc
    68701 tttttgcttg cgctgcaggt ttatgaggat aaataacttc gattgctaaa actttccctg
    68761 tcgcatctac gactgctagt ttacatccag tacgataagc cggatcaaat cccaggacaa
    68821 ctttaccttt tagtggcggt tgcaacaaaa gattacggag gttttcgccg aaaatagcga
    68881 ttgcttgctc atctgccttt tcagtcaatt cattgcgcag ttctcgctcg attgctggct
    68941 gaataaagcg tttataacta tcctgatagg cttctctgac gaatgaagaa gaaggagagg
    69001 caggattttt gactatttgg cgatccaagt aagctaaaat tgctgcttca tctacttgaa
    69061 agaatacttt cagaatatct tctttttctc cacgattagt agctaatatt cgatgagaaa
    69121 ccattttatg gacgggttct gcaaagtcat aatacatttc atatacgcct ttttcatctg
    69181 cttgctcatc cttgacattg ctgacataca tacctttgtt atacgtataa gaccgaatcc
    69241 aagtccgaaa ttttgcatta tcgctgactt gttcagcaat aatctcatga gcaccgtgca
    69301 acgcttcttc ggcagaagag acttcttttt ctttgtcaat atatttttct gcttcagatt
    69361 gcacctctcc atctgtaagc tgcatcagcc acttcgcgag tggttcaagt cctttttctt
    69421 tagcaatcgt tgcttttgtc cgtcgttttt gtttgtatgg acgatacaaa tcttccactt
    69481 gttgcatttt cacggcttgg gtgatttctg cttctaactc gggagtcagt ttcccttgtt
    69541 catcgattag ccgaatcact tcagttttac gtttttctaa gttttcaaga taggcatacc
    69601 gctcttcgat ttcacgaatc tggacttcat caagacttcc agtcatttct tttcggtaac
    69661 gcgcgataaa aggaacagtg tttccttcat ttaacaaatt aagtacagta gtcaactgct
    69721 taggacgata gtctgaaagt tctttttgta caagctgaat gatcgtctgg ttcaaatttt
    69781 ccgtcattaa aatgcctctt ttctgctaaa aattgcttct ttattctatc atacttttta
    69841 tcctgggtta aactactgaa agaaacaata ttctctataa aaataaaaag agccgcaaca
    69901 aagtcacagc tccttcattc tttccattag tcagattcca tgccaaggtc cttcgttgtt
    69961 ctgatattca atatcttgaa gaataccttc cttgtcaaaa acaaccccat gcaatgaccc
    70021 tccgaataca gccccaccgt ccattccgat tttatgatcc tgaatccaga ggtctgttgt
    70081 ctgcatatct ccatggagca ttggtgtaat cgtatgtcca aatacaattg tcttacctgt
    70141 attgtttttc ccttcatgaa atggttccct tatccaaata aagtctctta gacttgtctt
    70201 acgccagtct ttctttgtca aatccacacc ggcatggaca aaaagataat tttcccattc
    70261 ataatacaag gggcgctgga ctaaaaattt aatcagttct gaatatctcg attggatcat
    70321 catcgagatt tcagttgggg agtattcctc cgatgcccct gggtgaatca ggctttcaaa
    70381 cgtttctttt ccaccatttc tcatataagg cgcataccag tcctccggtg attgcagaaa
    70441 ttgcaaaaaa tattcttcat ggtttccccg gaggtaaatg gctcctgttt cttcgactaa
    70501 ttgctttcct aataaaaaac attcttttgt acgaggacct cgatcattga gatcccctaa
    70561 taaaacaagc tgatggatct ttggatcata atagtgaata agtttttcaa aaagctcatg
    70621 tttgccatgg acatccccaa tagcataaat caatgatttc atgtggatca ccatcctcaa
    70681 cgctagtata gcgcatattg gaaatggtcg acaatcaaaa ctattgtttt ctatttaatt
    70741 aaagctgtaa accgcagttc tgcttcttct ggagagattc catggtcttg aacatagcga
    70801 ttgcgcggat gtttcgcaca ctctatgcta catgctccta aatgtttttc ttcattttca
    70861 atggaagcaa gaatctgacg attgcactca ggattgctac aattgatata tcgttcacat
    70921 ggtgttccgt caaaccagtc gcgaccgacg atttgtttat ctacttggtt gatatcgaca
    70981 gcgatccgtt catcaaacac atacatcttg ccgtcccata attctccttg gacttcagga
    71041 tccttaccat aagtagcaat accgccatgc agctgtccga catctttgaa tccttcacgg
    71101 acaagccaac cagaaaactt ctcgcaacga atgccaccag tacagtaggt cactacccgt
    71161 ttttccataa attcttcttt gtgctcgcgg atccagtctg gaagctctcg aaaactgcgt
    71221 atatctgggc gtacagcccc tcgaaaatgt cccaagtcat attcataatc attccgtgca
    71281 tcaatcacca ctgtctgttc atctaaaata gcttctttaa attcttgcgg tgaaagatag
    71341 gcacctgtca cttggtttgg atcaatgtcc tcttctagct ttaaacttac aagctctgga
    71401 cggtatctta caaacatctt cttaaaggca tgtccttcgc ttggatcgat cttgaacatc
    71461 gtatcagcaa atcgaggatc agctttcatt gctttcatat aggcagtcgt gttttcaacg
    71521 gttccagaca cagtaccatt tattccttct tcagctacta gaatacgacc tttaagatcc
    71581 aaagatttgc aaaaggctaa atgatttttg gcaaattcct ctggttcttc gatttttgta
    71641 taattgtaat aaagcagtac ttgataatcc atgtatttct tccttccttc ttttcgttcc
    71701 taaacagtat aaagaaggcg ttcgcatttg acgatttatt tgcttgtgta taattacgca
    71761 tggaaagttt tttgtttaca taatattatt atttgaatta aaaagacttc gctttcctgt
    71821 ttcgcacaga aaaacaaagt ctttatttct attatttacc tgtaaccgca aagtagttgc
    71881 catcgggatc agcaaagtta aagacatatt cttctccaag ctgtaccata tcacctaatg
    71941 tgactgattg cgtctgcatt tttttatata gtccaaagat atctttactg taaaacatca
    72001 aagatggggc actagtagct gtgccaggtg aatgggactc aacaaatgct cgatcatata
    72061 acagaaatga ggtttgactg tcctcaactc ctacttcaat tataagcgtc ccatccattt
    72121 cttgacgatc tttttccgca aagccaactg ctctccaaaa cgttgcattt ttttctacat
    72181 cgtccacata aagcatgatt tttatttcat tcgtaaacat ct
//
LOCUS       DACECR010000003        63403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.003, whole genome shotgun sequence.
ACCESSION   DACECR010000003 DACECR010000000
VERSION     DACECR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.003"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            83..970
                     /locus_tag="IWO17_000144"
     CDS             83..970
                     /locus_tag="IWO17_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0289050.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IWO17_000145"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IWO17_000145"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ0289051.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IWO17_000146"
     CDS             2272..2718
                     /locus_tag="IWO17_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0289052.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IWO17_000147"
     CDS             2991..4343
                     /locus_tag="IWO17_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ0289053.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IWO17_000148"
     CDS             4514..4801
                     /locus_tag="IWO17_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289054.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="IWO17_000149"
     CDS             5027..5470
                     /locus_tag="IWO17_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0289055.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="IWO17_000150"
     CDS             complement(5476..6660)
                     /locus_tag="IWO17_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ0289056.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="IWO17_000151"
     CDS             6928..7626
                     /locus_tag="IWO17_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ0289057.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="IWO17_000152"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="IWO17_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ0289058.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="IWO17_000153"
     CDS             complement(8436..8969)
                     /locus_tag="IWO17_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289059.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="IWO17_000154"
     CDS             complement(9266..11647)
                     /locus_tag="IWO17_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0289060.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IWO17_000155"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IWO17_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ0289061.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12887
                     /locus_tag="IWO17_000156"
     CDS             12348..12887
                     /locus_tag="IWO17_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ0289062.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12977..13396
                     /gene="gpsB"
                     /locus_tag="IWO17_000157"
     CDS             12977..13396
                     /gene="gpsB"
                     /locus_tag="IWO17_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ0289063.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13418..13785
                     /gene="rnpB"
                     /locus_tag="IWO17_000158"
     ncRNA           13418..13785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWO17_000158"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13994..15160
                     /locus_tag="IWO17_000159"
     CDS             13994..15160
                     /locus_tag="IWO17_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ0289064.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15206..16702
                     /locus_tag="IWO17_000160"
     CDS             15206..16702
                     /locus_tag="IWO17_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ0289065.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16783..17466
                     /locus_tag="IWO17_000161"
     CDS             16783..17466
                     /locus_tag="IWO17_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0289066.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18111..19301
                     /locus_tag="IWO17_000162"
     CDS             18111..19301
                     /locus_tag="IWO17_000162"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ0289067.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19403..20356
                     /locus_tag="IWO17_000163"
     CDS             19403..20356
                     /locus_tag="IWO17_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ0289068.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSGNVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20425..21774
                     /locus_tag="IWO17_000164"
     CDS             20425..21774
                     /locus_tag="IWO17_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0289069.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21957..22280)
                     /locus_tag="IWO17_000165"
     CDS             complement(21957..22280)
                     /locus_tag="IWO17_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289070.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22409..23572
                     /locus_tag="IWO17_000166"
     CDS             22409..23572
                     /locus_tag="IWO17_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289071.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23642..24991
                     /locus_tag="IWO17_000167"
     CDS             23642..24991
                     /locus_tag="IWO17_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0289072.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25036..25467)
                     /locus_tag="IWO17_000168"
     CDS             complement(25036..25467)
                     /locus_tag="IWO17_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0289073.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25855..28497
                     /gene="alaS"
                     /locus_tag="IWO17_000169"
     CDS             25855..28497
                     /gene="alaS"
                     /locus_tag="IWO17_000169"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ0289074.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKEHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28697..29293
                     /locus_tag="IWO17_000170"
     CDS             28697..29293
                     /locus_tag="IWO17_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0289075.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29415..30563
                     /locus_tag="IWO17_000171"
     CDS             29415..30563
                     /locus_tag="IWO17_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0289076.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30601..31014)
                     /locus_tag="IWO17_000172"
     CDS             complement(30601..31014)
                     /locus_tag="IWO17_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ0289077.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31093..31524
                     /locus_tag="IWO17_000173"
     CDS             31093..31524
                     /locus_tag="IWO17_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ0289078.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31623..31856)
                     /locus_tag="IWO17_000174"
     CDS             complement(31623..31856)
                     /locus_tag="IWO17_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ0289079.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32039..33709
                     /locus_tag="IWO17_000175"
     CDS             32039..33709
                     /locus_tag="IWO17_000175"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ0289080.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33856..34242
                     /locus_tag="IWO17_000176"
     CDS             33856..34242
                     /locus_tag="IWO17_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289081.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34229..34927
                     /locus_tag="IWO17_000177"
     CDS             34229..34927
                     /locus_tag="IWO17_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0289082.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34932..35396
                     /locus_tag="IWO17_000178"
     CDS             34932..35396
                     /locus_tag="IWO17_000178"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ0289083.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35393..36304
                     /locus_tag="IWO17_000179"
     CDS             35393..36304
                     /locus_tag="IWO17_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0289084.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36539..37078
                     /gene="pyrR"
                     /locus_tag="IWO17_000180"
     CDS             36539..37078
                     /gene="pyrR"
                     /locus_tag="IWO17_000180"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ0289085.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37092..38375
                     /locus_tag="IWO17_000181"
     CDS             37092..38375
                     /locus_tag="IWO17_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ0289086.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38388..39314
                     /locus_tag="IWO17_000182"
     CDS             38388..39314
                     /locus_tag="IWO17_000182"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ0289087.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39333..40613
                     /locus_tag="IWO17_000183"
     CDS             39333..40613
                     /locus_tag="IWO17_000183"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ0289088.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40618..41694
                     /gene="carA"
                     /locus_tag="IWO17_000184"
     CDS             40618..41694
                     /gene="carA"
                     /locus_tag="IWO17_000184"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ0289089.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41699..44884
                     /gene="carB"
                     /locus_tag="IWO17_000185"
     CDS             41699..44884
                     /gene="carB"
                     /locus_tag="IWO17_000185"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ0289090.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44938..45714
                     /locus_tag="IWO17_000186"
     CDS             44938..45714
                     /locus_tag="IWO17_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ0289091.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45711..46643
                     /locus_tag="IWO17_000187"
     CDS             45711..46643
                     /locus_tag="IWO17_000187"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ0289092.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46640..47356
                     /gene="pyrF"
                     /locus_tag="IWO17_000188"
     CDS             46640..47356
                     /gene="pyrF"
                     /locus_tag="IWO17_000188"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ0289093.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47363..47992
                     /locus_tag="IWO17_000189"
     CDS             47363..47992
                     /locus_tag="IWO17_000189"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ0289094.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47980..48294
                     /locus_tag="IWO17_000190"
     CDS             47980..48294
                     /locus_tag="IWO17_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289095.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48332..48973
                     /locus_tag="IWO17_000191"
     CDS             48332..48973
                     /locus_tag="IWO17_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0289096.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49004..49708
                     /locus_tag="IWO17_000192"
     CDS             49004..49708
                     /locus_tag="IWO17_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ0289097.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            49705..50607
                     /locus_tag="IWO17_000193"
     CDS             49705..50607
                     /locus_tag="IWO17_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0289098.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50765..52471)
                     /locus_tag="IWO17_000194"
     CDS             complement(50765..52471)
                     /locus_tag="IWO17_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ0289099.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52661..53284
                     /locus_tag="IWO17_000195"
     CDS             52661..53284
                     /locus_tag="IWO17_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289100.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53734..54783
                     /locus_tag="IWO17_000196"
     CDS             53734..54783
                     /locus_tag="IWO17_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0289101.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54780..55661
                     /locus_tag="IWO17_000197"
     CDS             54780..55661
                     /locus_tag="IWO17_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0289102.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55664..56434
                     /locus_tag="IWO17_000198"
     CDS             55664..56434
                     /locus_tag="IWO17_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0289103.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56713..57567
                     /gene="aroE"
                     /locus_tag="IWO17_000199"
     CDS             56713..57567
                     /gene="aroE"
                     /locus_tag="IWO17_000199"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ0289104.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57745..58908
                     /locus_tag="IWO17_000200"
     CDS             57745..58908
                     /locus_tag="IWO17_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0289105.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59025..59435
                     /locus_tag="IWO17_000201"
     CDS             59025..59435
                     /locus_tag="IWO17_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0289106.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59609..61837
                     /gene="topB"
                     /locus_tag="IWO17_000202"
     CDS             59609..61837
                     /gene="topB"
                     /locus_tag="IWO17_000202"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0289107.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            61975..62292
                     /locus_tag="IWO17_000203"
     CDS             61975..62292
                     /locus_tag="IWO17_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289108.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            62452..62688
                     /locus_tag="IWO17_000204"
     CDS             62452..62688
                     /locus_tag="IWO17_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0289109.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62685..63084
                     /locus_tag="IWO17_000205"
                     /pseudo
     CDS             62685..63084
                     /locus_tag="IWO17_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
BASE COUNT        20986 a        10952 c        13565 g        17900 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
    12661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
    12721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
    12781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
    12841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
    12901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
    12961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
    13021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
    13081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
    13141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
    13201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
    13261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
    13321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
    13381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
    13441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
    13501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
    13561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
    13621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
    13681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
    13741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
    13801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
    13861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
    13921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
    13981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
    14041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
    14101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
    14161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
    14221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
    14281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
    14341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
    14401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
    14461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
    14521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
    14581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
    14641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
    14701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
    14761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
    14821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
    14881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
    14941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
    15001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
    15061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
    15121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
    15181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
    15241 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
    15301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
    15361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
    15421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
    15481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
    15541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
    15601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
    15661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
    15721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
    15781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
    15841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
    15901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
    15961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
    16021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
    16081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
    16141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
    16201 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
    16261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
    16321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
    16381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
    16441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
    16501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
    16561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
    16621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
    16681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
    16741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
    16801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
    16861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
    16921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
    16981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
    17041 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
    17101 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
    17161 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
    17221 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
    17281 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
    17341 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
    17401 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
    17461 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
    17521 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
    17581 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
    17641 cggaattggc aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc
    17701 acagtccttt tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt
    17761 aaaaattgct gataaacgaa gatggaaatt ttatgtattt ttattggtct atgcaataaa
    17821 taaattaaat attcagaata caattgattt taacgaaagg gcttctatca atggttgtgt
    17881 tgtatttatc tgcccaggta gagtaaaata gatcattatc taaaaggtaa ttgttcatac
    17941 tatataagtc atttgaaagt aaaaagatac tgtaaataaa aaatgtggtt aaataattct
    18001 cataatcagt gtaagcgtta tcaaaaatat agcatacttg ctttgtagat gaatgacacg
    18061 atatatgttt ggtatgttct gcaagacaga tgaatatgga ggtggagaca atgtcttcca
    18121 aagaactaac aatgtattta gagaaagaat tggatcaact gaaagaaaag gggttataca
    18181 atacgatcga catactagaa agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga
    18241 tgatcaatct agcatccaac aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag
    18301 catgtattga agcaaccgaa acttatggtg taggagcagg agcagtccga acgatcaatg
    18361 gttcgctgaa aatccatcag caattagaag aaaaaatcgc agaattcaaa gggacagaag
    18421 cagcaatcgc ctttcaatct ggattcaact gcaatatggg cgctatctct gcagtaatga
    18481 caaaagaaga tgctatctta tcggatgaat taaaccatgc atctattatt gacggttgcc
    18541 gcttatccgg agcaaaaatc atccgaatta aacatcaaga tatgaaagac cttgagaaaa
    18601 aagcaaagga agcgatagaa agtaagaaat ataaaaaaat catgtatatc acagatggtg
    18661 ttttctccat ggatggagac attgcacgat tgccggaaat tattccgatt gttgaaaaat
    18721 atggactgat cacctacgtc gatgatgcgc acggctcagg cgtaactgga aaaggtgcag
    18781 gaaccgtcaa gcattttgga ttaagcgaca aaatcgatat gcaaatgggc acgttatcga
    18841 aagctatagg tgttgttggg ggttatgtag ctggatcgaa aacactaatc gactggttga
    18901 aagctagaag tcggccattc ctattttcta cttcgctaac tccaggtgca gctgcagctg
    18961 cattggcttc gattactttg atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa
    19021 atgctaatta ttttaaagaa gaattgaaaa aagtaggcta taacatcggt atgtctgaaa
    19081 cacctattac tccagtcatt ttgggagacg aaaaagtgac tcagactttc tctaagaagc
    19141 tgatcgaaca tgggatttat gctaaaccaa tcgtttatcc aactgtgcct cttgggacag
    19201 gcaggatcag aaatatgccg actgcggaac atacgaggga aatgcttgat gaagcagtag
    19261 cagtttatca aaaaatagga aaagaaatgg aaattattta actaaattta gttttttcgt
    19321 ctatttaaaa ttagagaaca tgttcacgca tatattgtta atcatctgaa gatagtagta
    19381 aaaaagatca gggaggatca tcatgaaaaa agtattggtg acaggatgtt taggacaaat
    19441 tggttcagag ttgatcacac ggctacgtca agaactaggc agtggaaatg tcattgcgac
    19501 agatatacgc atgcctgaag tcattgagaa aaacgatatc tttgagatcc tagatgtaat
    19561 ggattacgaa aaaatgcgca gcatagcaga acaatatcaa gtggacacat tgattcattt
    19621 ggctgctctt ttgtcagctg ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt
    19681 gactgggctg gtcaatgcat tggaagtcgc tagagaattc cagctgaaat tttttacgcc
    19741 aagttcaatc ggcgcgtttg gtccaaatac tccaaaagat cataccccac aagatactct
    19801 tcagcgtcct acaaccatgt atggggtaac gaaggtagct ggagaactac tatgtgatta
    19861 ttattatgtg aaatatggcg tagatactcg tggtgtccgt ttccctggat tgatttctta
    19921 tgcaaccttg ccaggaggcg gtacgacaga ctacgcagtc gatatttatt attcagcaat
    19981 caaagaagga aagtacacta gtttcattga tcaaggtaca tcaatggata tgatgtatat
    20041 gccagatgcg atcgatgcca ttataaaact catgaatgca gatcctgatc gattggttca
    20101 tcgaaatgcc ttcaatatat cggcgatgtc ttttgacccg gaagagatca aagcatctat
    20161 tcaaaaaatc ataccagatt ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat
    20221 agcagaatct tggccaaatt cactagatgt ttcttgtgcg caagaggaat ggggcttttc
    20281 gccgaaatac gacttagatg tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt
    20341 ggtagagacg gtataaaaca agaaccacat ccaaaaagtt ttttatactt ttgggattgg
    20401 ctatggtatg aaaggggaca agggatggaa gaatatcaag ccacgccagt aaaagaagta
    20461 aaaatagaaa atgaattaaa acgtacgatg ggatttttta ctgctttatc gactgttatg
    20521 ggcacagtga tcggtgcagg ggtatttttt aaagccgcaa gcgtagcaga agttacagga
    20581 tccgccagtt tgcatatgtt ttcttggttt ctcggtggta tgatttctgt ctgtgcagga
    20641 ctaacaggag ctgaactggc agctgctatt ccagaaacag gagggatgat taaatatatc
    20701 gaacggattt acggaaatac agcagctttt ttattgggct gggcgcaagt cgtgatttat
    20761 tttccagcaa atgtagcagc cttgtcgatc attttcggta cacagttcgt caatctattt
    20821 ggtctttccc agtcaatgat tgttcccgta gctgttacag ctgctgtatc gatcctgttg
    20881 atcaattttt taggttctaa agctggaggg gcttttcagt cgattacgct tgtctgcaag
    20941 cttattcctt tgtttgtgat cgtcattttc ggtttgttcc gacaagaagg agtagacttc
    21001 caactgtttc cgatccaagc aggagaaaat ctttcatttt tctcagcctt gggagcgggc
    21061 ttgctagcaa cgatgttcgc atacgacggt tggatacacg taggaaatat ttcgggagaa
    21121 ttgaaaaagc cggcgaaaga tttgccaaaa gcaatttctt taggaatcat cggaatcatg
    21181 atcgtttatt tattagtaaa tgcggtgttc ttaagaaccg catctatcga cggagtcgca
    21241 ggaaacagca atgcggcaag tgacgttgca aaaatgattt ttggcggctt cggtggaaga
    21301 ctggtcacag ttggtatctt gatttctgtt tatggaacca tcaatggcta tacccttact
    21361 ggaatgcgtc tcccttatgt tatggcaaaa gaaaacaacc tgccttttag caagctcttt
    21421 gctaaacttc atgataaaac taaagttcct gtagccgcag gtatcttaga gctagtcatc
    21481 gctattggca tgatgatgat cggtggtttt gatacactaa cggatatgct gatctttgtc
    21541 atttggattt tttatacgat ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct
    21601 gatttgtttc gaccatacaa agttccaatg tatccgttta ttccactagt agcaatcatt
    21661 ggagggactt ttatcgtatc aagcacgctt atcactcaga cctttttagc ttcgatgggg
    21721 atagtattga cacttgctgg tatcccgatt tatctctatt taaaacgtag atagctaaaa
    21781 cattcgaatt ttattagaac tgcacttctc tgccaaacaa aatattttat ttaaatgagg
    21841 agtacgtgga agaaatgtca aaagtactcg ttaattttga tcacgtaaat gtcgttttgt
    21901 ctacgatgaa atagcaaagt aaaagacaga aatcgggctc aaccgatttc tgtcttttac
    21961 tttgatttgc gtaactgaac tattcggcgt acgccgcgat aaatatttaa taaaaccaag
    22021 caaccagaaa tgaaaatggg aagccaaatc agatagacag ggtagccaag cactttagac
    22081 aaagcatgtg gagtcatttt atccatttca agaagtgctt gctgccaagt ctgaagattg
    22141 aagactagca tgaaacccga aataatcagg agaataaatc ctaaataatg aattgtagtt
    22201 ctttttctta cataagcgat cgtagcataa aggaacagta aaaaaatagg tacgccaaaa
    22261 acaaagtaca tattcagcat ctcgacaccc cctttgtgtt tatcttactt tactttgaaa
    22321 gaaatgagaa ataatagcct cgttacttgc ttgaaacagc aaaggactgc taagataaga
    22381 gagatacaaa gaatcaagga ggagtcatat ggcaagttat atcgaaccga ttcgttttgc
    22441 gctgattgtt tttccatttt tagccttagc gatctcatct gtttttttta tttatgaata
    22501 tcgaaagtat ggaacatttc tagtaacaag ggcagttatt ttgtattctt tcgtttttta
    22561 tttattgtgt gcgtattttt tggtgatctt accacttccg gcaaaggaga cggttgccca
    22621 aatgacaggg ccaaagatgg agctgcattt atttgcttca tggcaacatt tcagagacaa
    22681 cacggtattg gtcttgtctg aacctagtac ctacctgccg gcaatgaagc agagtgtatt
    22741 tttggaacca gcttttaatg ttttattact cataccattt ggcatttatt taagatatta
    22801 tttccggctt tcttttatga aaacgatgat tgctagtttc tgcctttctc tattttttga
    22861 attgacgcag ctaagtggac tttattttat ctacccaaga ccataccgct tatttgatgt
    22921 gaatgattta gtgacaaata cattgggtgg aatcattggt tttgtcgtta cgccgttatt
    22981 tacatttatg cttcctacaa gaagacgaat ggatgaactt tcttatgaaa aaggaaagac
    23041 tgtctcattg actcgacgct tggtcgctta tttgatcgac tggacatttt tatctcttat
    23101 taatttggta gcggcgattt tctttaaaat tgcgttcgat tcaggggcag ttacttctca
    23161 tatctggtgg cttttattag aagtaggggt atactttatg attgtaccgt ttataaccaa
    23221 tggatggaca cttggcaaaa aaattgtgcg tatccgtttg gtggaagaag accgcgaacg
    23281 aatttcttta aaagcattga tgatccgcta cgggtatctg tactttcttt tttatggttt
    23341 aagctggttg gggcagttgc ttcagcaaat catgaattcg acaacgaata cggtgttgca
    23401 gatgagtgca gtattcattt ctctcggtgt cttgttacta caaggtattt ttattttaaa
    23461 tatcttgcta tcatttttga gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac
    23521 atatactgtt agcacgattg gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa
    23581 aaacgctttg tctgtgttat aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg
    23641 aatgaataat tttgatatat tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa
    23701 gggcttcaaa cagcccacag aggtccagga acgtttgatt cctttaattg caaaaggaaa
    23761 aaatatcatt gggcaatctc aaactggtac aggaaaaaca catacgtttt tgttgccttt
    23821 gtttgaaaaa atcaatgcag aaaaagagga agtacaagtt gttattacag ctccaagtag
    23881 ggaactagct gcacagatct atgaagcagc tacacaaatc gctaaatttt ctacaccgga
    23941 gatccgtgtc tcgaattttg tcggcggtac agacaaacag cgtcaattaa atcgtctgca
    24001 tcaccaacag ccacaggttg tagtaggcac gcctggtaga atattagaca tgatgaatga
    24061 acaagcgctg aagattcaca ccgcatttgc atttgttgtc gatgaagcgg atatgacatt
    24121 ggatatgggt tttttaaatg aagtagacca aattgcaagt cgattaccag aaaagctgca
    24181 gtttttagtt ttttctgcca ctattccaga aaaacttcgt ccattcttac gaaaatacat
    24241 ggaaaatcca atcattgaag aaatcaaacc aaaagcagtt atttctgaag acattgaaaa
    24301 ttggctgatt tcaacaaaag gtaaagatga gaatcaaatg atttatcaac tgctgacaat
    24361 tggtcatcct tatttagcaa tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc
    24421 cgattacttg aaaaaccaag gcttgaaagt agcaaagatt catggagaca tcaccccaag
    24481 agagagaaaa cgagtgatgc gacaagtaca gaatctggag tatcaatatg ttgtagcgac
    24541 tgatttagct gctagaggga tcgatatcga aggtgtctct catgttatta atgcagaaat
    24601 tccagaagat ctagacttct ttatccatcg tgtcggacgt actggacgta atggcttaaa
    24661 aggaacggcc attacgttat attcgccaaa tgatgagcaa gccattgatg cgatagaaaa
    24721 attgggcgtc tcatttgaac caaaagaaat caaaaacggg gaaatcgtca aaacatacga
    24781 tcgtaataga cgtgccaaac gagaaaaatc aaaagatgta ttagatccca cgctgattgg
    24841 tcttgtgaag aagaaaaaga aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc
    24901 aattgcagaa aaaaataaaa aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc
    24961 tcgtaaaaat aagaaaaatt cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt
    25021 cacaatctct tgtttttaag ctagactttt tttcattccg atacaaagta cttcatcttc
    25081 aataaaaggt tctgtgcaag tatgataccc ttgtttttca taaaaatcta cagccgtcat
    25141 ttcagctact acataactag tctgacaacc ttctgaaacc gcccgttttt cagcagcttc
    25201 aagcaactga ccgcctaacc cttttcctcg gtattctttt gctacgcaaa atcgttccgg
    25261 cttgaagcaa gtaggtgttt tcttttcata acgcaaagta gctactggtc ttttttgatc
    25321 aagaaataga taataggttt gttgcgggat atcttttccg tcgaattcta gttctgcagg
    25381 aatcccttgt tctaatacaa aaacttgata tctcaaaaag cagctagctg cgcgtatcca
    25441 ttcatccgct ccaactattt ttttcatcat attcctcctt gtcatgaata gcagagatgt
    25501 attgattcct ttattcttat cgtaccaaaa atcgtggagt atgaccaaaa ggaaatgaaa
    25561 ataaaaattg agatattgac agtgatcaat agaatgcgta tactaaataa gtacgaaaag
    25621 gatatgattt gttttttaag actcacagaa aattctttct tggctgaaaa aagaatagtc
    25681 actaatcaaa ttgctccctt tttctatttt ggtagaaata ccgacgcagc tcagcgttat
    25741 cgagtttaag aggtaatgat tacacatcat tgcaatcaag gtggtaccgc gagttttcgt
    25801 ccttggctgt gatggtgttt ttttgtattt aatttataga aagcaggtaa tgacatgaaa
    25861 caattaagta gtgcacaagt tcgacaaatg tttcttgatt tcttccaatc taaagaacat
    25921 acgattgaac ctagtgcgtc tttgatcccc gtgaacgatc caaccttatt gtggatcaac
    25981 tctggtgttg caacattgaa aaaatatttt gatggttcag ttgtgccaga aaatccaaga
    26041 atcacaaatg cccaaaaatc tattcgaaca aatgatatcg aaaatgtcgg taaaacagca
    26101 cggcaccata cgatgtttga gatgctgggt aatttttcga ttggtgacta tttcaaaaat
    26161 gaagccatcc actgggcatg ggaatttttg actgatccaa aatggatggc attcgatcca
    26221 gaaaaacttt atgtaacagt ttatccaaaa gatacagaag caaaacgcat ctggcatgaa
    26281 gaagttggcc tgccactaga tcacattatt gatgtagaag acaacttctg ggatatcggt
    26341 gcaggcccat gtggtccgga tacggaaatt ttctatgacc gtggagaagc ttataacgat
    26401 ttagcagaag acgatcctga aaactatcca ggaggagaaa atgaacgtta tttagaaatc
    26461 tggaacttag tattttcaga attcaaccat aagcctgata acacatttga accacttcct
    26521 cataaaaaca ttgatacggg gatgggattg gaacgcatgg tctcaatcgt tcaagatgcg
    26581 tcaacgaact ttgagacaga cttgtttatg cctattatcc acgccgtaga aaagctaagc
    26641 aaacaagtaa aatatggtga aaacccagta acagatattt cttacaaagt aatcgcagat
    26701 catattcgcg ccctttcatt tgctattggc gacggtgcat tgccatcgaa tgaagggaga
    26761 gggtatgtct tacgccgttt gcttcgtcgt gctgtcatgc atgggaaaaa attaggtatt
    26821 gaacaagcat tcttatatcg tttagtccct gttgttgggg aaatcatggt aagttattat
    26881 cctgaagtac ttcaacaaaa agaatttatt gaaaaagtta tccgtacaga agaagaacgt
    26941 ttcaatgaaa caatcaatga aggtttggaa atcttaaatc aagtgatcca agaagtaaaa
    27001 aacgaaaacg ggacgactct agcaggaaaa gacatcttca aactatacga cacatatggc
    27061 ttcccggtag aattaacgga agaggtggca gaagatgaag gactgaaagt tgatcatgct
    27121 ggttttgaaa aagaaatgga tgcacaacgc gaacgtgctc gctctgcccg cagtactgaa
    27181 aaatcaatgg gggtacaatc tgctttattg acagatatca aagtagaaag taaattcatt
    27241 ggatacacag aacaagaatc agatagtcaa ttattggtca tcgttaaagg tgagtctctt
    27301 ttgaatgaag tcgcagaagg tgaagctcaa atgatctttg atcaaacacc tttctatgca
    27361 gaaatgggtg ggcaagtagc ggatcaagga tggatcatga atgaagtagg cgaaatcgta
    27421 gcagttgtta cagatgttca aaaagcgcct aatggtcaat ttatgcacac cgtagaagta
    27481 aaagcaccaa tgagtgaagg acaaacctat catctattga tcgatcaatc gatgcgcaat
    27541 cggatcatca aaaaccatac agcaacccat ctactgcaca aggcattgaa agaagtactt
    27601 ggcgagcatg caaatcaagc tggttcttta gtcgctcctg gacatttacg attcgacttt
    27661 acccactttg gtcaaatcac ttcagatgaa ttagctgaaa tggaaagaat cgtcaacgaa
    27721 aaaatctggg aagctatccc agttgaaaca attgaaacag atatcgatac agccaaaaac
    27781 atgggtgcaa tggctttgtt tggtgaaaaa tacggacatg acgttcgagt agtaaatatt
    27841 ggcggctggt caatcgaact gtgcggaggc acacatgtag caaatacaga aaacatcgga
    27901 atcttcaaaa tcgtttctga atcaggaatt ggtgcaggcg tgcgacgaat cgaggctgta
    27961 acaagtaaag aagcgtacga actacttgaa gaagaagaaa aacaactaaa acaagtagca
    28021 acaattgtta aatcacctca attgaaagaa gttgtttcaa aagcggaaca attgcaacag
    28081 caagtaagag agctacaaaa ggaaaatgaa caattagcta gcaagttagc gaatcaacaa
    28141 gctggtgatg tattcaaaga tccagaaacg atcaatggaa aaacaatcat tgcagcacaa
    28201 gtaaaagtga aagatatgaa tcagctgcgt caattagctg accaatggaa acaaaaaaat
    28261 atttcagata ttcttgtttt agcaaacgaa caagaaggaa aagtcagtct gctagcggca
    28321 atgacgaaag aatcaaatga agctggacta aaagctggcg acttgattaa agcaatcgca
    28381 ccgaaagtag gcggtggcgg tggtggacgt ccagacatgg cacaagctgg cgggaaaaat
    28441 cctgctggta ttccagatgc acttaaagct gcgaaagaat gggttgcgca acaatagggc
    28501 gtttacgttt gacagaaaaa caaaagttca aacattgaac gaaacatgat tatgggggcg
    28561 aagacaaaca agtcttgccc tcatactttt ttcaattgga tggcgcatct aatcaaatgg
    28621 tcggattcgg atggtcttta gttgacgaaa gtgacagaaa tttttacgat tagtataaat
    28681 aaaaaggggt gaaaaaatga atagtagaaa taagaccttt cgactagtct tacgtgcgat
    28741 cttgttagca atcatcatcg tacaagcgat ggttccttgg ctggggttta ttccattagg
    28801 attcattagt ttaacaatta tccatatcac agtaattatt gctgcagtag tattaggccc
    28861 aaaagatggc atggtgattg gcttgttttg gggaatcgct acaattgttc gtgcctatgc
    28921 catgccaaca acgccatttg atacattagt gttcacgaat ccgattatct cggtagttcc
    28981 cagagtactt gttggcttag tagcaggctt ggttttccac tggatttatc aaaaaaataa
    29041 atcaataacg atcagctcag cctttgcagg ggtactgggt tcattggtca atacagtctt
    29101 agtgttagga ttcatgggat tattttatac aggtgcaacg gcagatgcct atggtgtaga
    29161 ctctgctctt ttatttaaaa ccttagcagg ggttgcagca atcaatggga taccagaagc
    29221 aataggtgcg ggaatcataa caccattact agcaaaagcg ttatttgcag caacaccgtt
    29281 gaaaccagaa taatgaccat cttttaatag taataggacg cacgatagat agaaaaagga
    29341 agagaggaga ggaaaacaat cgtcctctcc atctttttac ctgagcaaca agcaaactag
    29401 aaggggtaaa aaacttggct aagaaacaag aagaaaaaaa gaaacaaata ccgcaacagc
    29461 ggacaaaaaa aggagtacta gcgggtatct taggattggt tacgaatctg atcttgtttg
    29521 tctctaagct aatgatcggg cttttttcag gaagtgtctc cattatggct gatgcagtga
    29581 acagtctatc agatactgct tcatccatat tgactttgat cggatttaaa attgctgcaa
    29641 agccagcaga tcaggaacat ccttatggac acgaacgctt cgaatatatc agtgggttat
    29701 ttgtttcgat catcattact tatgtaggat ttcaattttt agagtcttct gctgaaaaaa
    29761 tcatccatcc agaaaatgtc tctttaacac ccatcgtctt ttttgtccta atcatttcca
    29821 tctttttgaa atttgcacaa ggcaaaatgt atcaggttat tgcgcaatcc attgattctg
    29881 aaacattgag agctacagca acagatagtt ataatgatgt ctttactaca ctggctgtat
    29941 tgctttcagc agcaatcgaa tgggtaactg gatggcgtat tgatggatat ataggttttg
    30001 ctttagcatt atatatcctt tatagtggat tgacgatgct tcgaaatttt gtatacgaac
    30061 tattaggaag tcgcccaacg ccagcagaga ttgaagaaat ggaagagcat ttaaatgctt
    30121 gcgatgatat acttggctat catgatttat tggtgcataa ctatggaccc aataaacgct
    30181 ttgcatcagt ccacatcgaa gtagatgaat caatgaattt gaatgtggct cataagatta
    30241 ttgatctgat agaaaaagac tttaaaaagt ctttggatgt ggaacttgtc tgtcacttgg
    30301 atccaatgcc tgttgataac gaaaaatact ataatatctt gcggcagtta aagcaaattc
    30361 ttcttgaaac aggtacaggg cttaatattc atgattttag agtaattcat caaggaaaaa
    30421 cgctgcaatt tgatgtagtg gtccctgaga aattccagtt tgttgataca gaactagaaa
    30481 gaatcttacg tagtcaaata gaagaaaaaa tcggagatta ccaattggaa attacgtttg
    30541 atcacaatta tcttttatat taaatgagaa actataaaaa gactccttaa agtttttaat
    30601 ttaaagagtc tttttttctt ttaattgttt atggagagcc tgacgtgcaa gattatctgc
    30661 tcctttattt ttgttttcgg gcaaccattg caggatcaat aaagaaaatt cggatagaag
    30721 ttcttggatt ctttgaaggt agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac
    30781 cgtagaaact agtgttttgc tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt
    30841 tttctttaga aattctaaag tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc
    30901 tatagaaagc gggatagata gctgttcttg tttaccttga tagacaatca agattccacc
    30961 tccactagga ccagggttgc cttttgtaga ggcatcaatg tatactttta acataaaaag
    31021 agattctcct ttttgttcaa actaatgtta tggtaagatt atatcattac tgaatcggtg
    31081 agaggttaga gaatgaaaaa aaatattcga tggcagcctg aactggcaca tacgatcatc
    31141 tattggtcat tgaccttgat cattctgttt tttagtctga ttctttcttt agaaaacaca
    31201 ggtccttatt ggaaaagcaa ttttgtttta ggtatttttc ttgtttttct atttctaggt
    31261 ttgcggcgtt cttttatttt aaaatcaaac tctgtgatta tacgttatgc tgctttttgg
    31321 aaagaccgag aaatatctgt cggacagatt cgatctttga cgccaactaa tactggggtg
    31381 caaatgatgt tgcaagatga gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa
    31441 gtagtccttt ttgatcattt tgaaaaaaac ggacccaaat cagaatcaat tccttatcct
    31501 tatacgaaaa aaagaaaaag ctaattttaa aaatcagaac ctaaaaaaac gaatttttac
    31561 aggaaaagaa aagagtgaga cgtacggttc atcccgttta gcgtctcact ctatttgttt
    31621 atctattcat gcatactagt atcggtagtc tgaacgttgc ctttcttgac atgggcagct
    31681 tgtgttcctc gattcccttc gatgatctca aattcgacca cttgattttc ttccaaagtt
    31741 ttgaatccct cttcttcaat cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa
    31801 ctgataaagc cgtatccttt tttgttgtca aaccatttta caattcctgt ggtcaaagca
    31861 ctcacgacct ttctaaagtc tgatcatagc cttcattata aagagtattt ataagtacgt
    31921 caaataaatt aaaaacctgt aattttatgt tttttttctg aataaaaaaa ggtataattg
    31981 gcatatctta acaaagaaga tattttgttg aataaagttc gtgaaaaggg gtaatcaaat
    32041 gaagaatgat ctgcaaattt cacaagaaac agagctagaa ccgattgttg aagtggctag
    32101 taaattagat cttaaggaag acgatttgga actgtatggg aaatataagg cgaaaatcaa
    32161 tttttctgga atcaatcgat caaaggataa cgctgagggg catttgatct tagttacttc
    32221 catcaatcca acaccagctg gcgaaggaaa gtcaacgatc actgtagggt taggagacgc
    32281 gctcaataaa atcaataaaa aatctgttat tgcattgcga gaaccctcac ttggtcctgt
    32341 gatgggaatc aaaggtggtg ctgcaggcgg aggatatgca caggtattgc ctatggaaga
    32401 tatcaacttg cattttactg gtgacatgca tgctatcaca acagctaata atgcattatc
    32461 tgctctttta gataatcata ttcatcaagg aaatgaattg aacatcgatg cccgccgagt
    32521 gatttggaaa cgggtagtcg atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt
    32581 gggtgggcca atccaaggtg ttccaagaga agatgggttt gatatcacag ttgccagcga
    32641 aatcatggct atcttgtgtc ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag
    32701 gatcgttgtc ggctatacat ttgaccgtca gcctgtgact gctggagact tgaaagcaga
    32761 aggcgctctg gcattactgc tgaaagatgc aatcaagccg aatcttgtac aaacaattta
    32821 cggaacacca gcttttgtcc acgggggacc gtttgccaat atcgctcatg gctgcaatag
    32881 tattttagct accaagacag cattgagatt agcagattac gttgtcacag aggcaggatt
    32941 tggtgcagat cttggcggag agaaattttt agatatcaaa gtacctaact tgaaaaaagc
    33001 gccggatgct gtggtgattg tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa
    33061 aaaagatgaa cttaaaaatg agaatcttga tgctttgaaa gtaggttttg caaatttaaa
    33121 gcgccatata cgtaatatgg aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt
    33181 cgtaacagat acagacaatg aactcacact gttggagcac ttgtgtgaaa atcaggggat
    33241 tcttgccaag agagcgagtg tatgggcaga tggagcagaa ggcggcgttg atttagcaaa
    33301 agctgtagta cgattgattg atagaaaaga agctaattac aagccattat accgcctaga
    33361 agaaacgata caagaaaaga cagaaacaat cgtcaagaaa atttacggag gaaatggtgt
    33421 tgttttcagc aaaaaagcgc aaaaacaaat agaagagttt acgaagaatg gatgggacaa
    33481 actgccgatt tgtatggcaa aaacacagta ttcattttct gatgatcctt cactgctagg
    33541 tgcaccggaa gatttcacca tcacgatccg cgaaatcatc ccaaaattag gggcaggatt
    33601 tttagtagcc ttgactggag atgttatgac gatgcctggt ttgcctaaaa aacctgctgc
    33661 attgaatatg gacgtgacaa atgacggaga agtgttggga ttgttttaag cttgattata
    33721 atctaccatc gaaggagctg tattttgact acagcttctt tttttagacg atagggaatt
    33781 aattttgttt acactaggat cattcttttt ctttattgta ttttgagcta tactaaagca
    33841 taataggagg gtatgatgaa aacgacattt ataagaaaaa ccggttttta cggtatgggt
    33901 agctcgattc agctaagaat caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag
    33961 ataacgcttg atctggaagt gccctttacg atgcaggtca ccttttattg gctaaaaagc
    34021 cccgtttata cagtgaaaga agcaaagcct gcttatgtga tcacaatgaa ttacttgatt
    34081 cttcaaatct atccgttttt atttctagca actgggttgg gggccgtttt cctacaaaat
    34141 ttatttttca gtttttttgc agttattgtg ttgattgcgt tctttttcta tataaaaaac
    34201 cgggcatatt tgatcaagga ggaaaattat ggcaaactct gatctttttt taatcagttt
    34261 caatcgaatc gaaaaatgga tgagagacga gatgggaaat gcacgaaata tgggatttac
    34321 cgaacttgtc agacgcctag ctcaaagaaa acaattaatg atcagaaaat acgaagatga
    34381 tcttttacag cttgctcagt taaggaatgc gatcgtccat gatcggatcg cagtcgattt
    34441 tgtcattgca gagccaaatg attggacagt caagcgaatc cagctgatcg aacaagaact
    34501 gattcagcct gaaaccgtgc tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga
    34561 tcttccgtta cttgaactgt taaagatagt agcagaaaaa aggtattcac aatttccgtt
    34621 atacaataaa ggaaaatttg tagcattgat tactttacgt aatatcgggt tttggctagc
    34681 aaaagaaagc caaaaaggtc cggttgattt aacaaataaa cgagcgttgg atttgatcat
    34741 ccaaaacgga aagtacacaa attatcattt tgttccagct aaaacacata tttacgaggt
    34801 tgaagcgatg tttcgtgagc aagggacttt ggaagccgta cttatcacta aagatgggaa
    34861 tcccgatggt aatctgctcg ggatcgtacg acctcgtgat atttataaac aaatagaaaa
    34921 ggattgattt tttgttaatt atttattgga tattcagcgc tttgctagta ggagtggatc
    34981 agttggtcaa atggtggatc actgataatc tagcgcttgg agagacgaaa aatcttatac
    35041 caggtatttt gtcactgaat catattcgaa acactggagc cgcatggagt atgctagaag
    35101 ggaagatgtg gttctttact atagtgacac tcattgcagt cgttgtgatt ctcacattga
    35161 tgattaaaaa tcgtgaaaaa ggtaatcgct ggttcatgat tggattgagt ttgattctag
    35221 ctggtgcgat tggcaatttt atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc
    35281 agacagattt tatgagcttt ccgattttta acgtagcaga tgtgacactt gtatgtggag
    35341 tgatttgtat gttaatttat attattttag atgaaaagga tcaaagaaaa aaatgacaga
    35401 acttaaaacg acgattcacc atgaatctgg taggatcgat aaagtattga ctaatttatt
    35461 caccgattac agtcgttcgc aaattcagct gtggctgaaa aatggagcgg tttctgtcaa
    35521 tggacagcca gtcaaagcaa attacaaagt aaaacaagga gacgagatta ctgtcttgat
    35581 ccccgaacca gaaacattag atgtggtccc agaaaatatc ccgttgacga tcgtttacga
    35641 agatgaagat gtagcggtga taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg
    35701 tcatacgcat ggcacaatgg tcaatgcgct gatgtaccat atcaaggatc tttctacgat
    35761 caacggcgtt attcgtccag gtattgttca ccgaatcgac aaagatactt ccggactgct
    35821 aatggttgcc aaaaatgacc aggcacacga atcattagct cagcaattaa aagataagac
    35881 ctctttgcgc aaatatgttg ctttagtcca tggagagatt cctcacgaaa aaggaaccat
    35941 caatgcgcca atcggccgtt cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg
    36001 gaagtcggct gtcacacatt ttactgtatt ggaacgtttc gaaggattta ctctggttga
    36061 attaaaacta gaaaccggaa gaacacacca gatccgtgtg catatgaagt atatcggtta
    36121 tccgttagct ggagatccag tatacggacc gaagaaaact ttgaaaggca atggtcagtt
    36181 tctccatgcg caaatgttag gttttacgca cccgactaca aacgaaaaga tgatctttga
    36241 agcacctctt ccagaagttt ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt
    36301 ttaatttaga taacggtata gtaattaaca acgaaagcat aaaacttaat agaattcctt
    36361 taagtttagt cccgtgaggc taagaagggt gggagcaagg atacgtatgg tgcgccataa
    36421 gtagattgct cattgacata gtatagctat cagtgtatca gtatacgctc agtctaaaca
    36481 ttcctcctgt cctcttaggg caggagtttt tttatgcaaa aaagtagggg gaatgaaaat
    36541 gcaagcaaaa gaagtagtag atcaggtaac catgaagcga gcattgactc gaattactta
    36601 tgagattatc gagagaaacc atagtatcca agatatcgta ttggtaggta tcaaaacacg
    36661 aggaatctac attgcatcaa gaattgccga acgcctgaag cagctagaag atatcgatat
    36721 tcctgtagga gaactggata tcactttata ccgagatgac aaaaaagaaa atccagaaga
    36781 accggaactt cattcttctg acatcccagt ttccttagaa ggaaaagaag ttattttgat
    36841 tgatgatgtt ttgtatacag gacgaacgat tcgagcagca atggacgcag taatggattt
    36901 tggcagacca agaaaaattt cattagcagt cctggtcgat cgtgggcatc gagaattgcc
    36961 aatccgtgca gattatgttg ggaagaacat tccaacatct cgggcggaag agattttagt
    37021 agagatgcaa gagcttgatg gacaagatcg catcatgatt ttgaaagagg aagattagag
    37081 gagagagaaa catgtcggag aaaaaaggat ttcataatga tgacgtggtg ttagatatcc
    37141 acgatcgccc aaccccagga cattgggtag gattaagttt acaacacttg ttcacgatgt
    37201 tcggagcaac cgtgttagtc cccatcttag taggtatcga tccaggtatt gcgctagtca
    37261 gttcaggact aggtacattg gtgtacttat ttactactaa aggaaagata cctgcgtact
    37321 taggaagcag cttcgctttc attgcagcga tgcagctttt gatgaaaaca gatggttatc
    37381 cagcgattgc acaaggagca gtcacaactg gattagtcta tttgattgtt gcgctgatca
    37441 tcaaaaagat cggttcagca tggctggata aaatcttgcc tccaatcgtc gtcggtccag
    37501 tcatcatggt tatcgggttg ggacttgcgg caaatgcagc aacaaatgct atgtataatc
    37561 agggcacgta tgatttcaag tatattgctg tagcgctgat cacattagga ttaacaattt
    37621 tcttcaacat gtggctaaga ggttttctcg gattgattcc gattttacta ggtattatta
    37681 gcggatacat cgtagccttg ctgtttggaa tcgtggatac taagccaatc gtagacgctg
    37741 cctggtttgc tgttcctaac tttgagattc cctttgtaca atatgaacca aagctatatc
    37801 ttggagcaat cacaacaatg gcacccatcg cttttgtgac gatgacagag catatcggcc
    37861 atcttatggt attgaataaa ctaactaaac gtaatttctt tgaagaaccg ggtcttcata
    37921 agacgttgac aggtgatgga ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg
    37981 taacaagtta cggtgaaaac attggtgttc tggctatcac acgtgtccat agcgtgtttg
    38041 tgatcggtgg agctgcagta tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt
    38101 tgattttaag tatccctgga ccagtcatct caggaatcag ttttatctta tttggagtaa
    38161 ttgccgcaag tggaatgaag attttaatcg agaacaaaat cgattttgat aaaaagaaaa
    38221 atctgttgat tgcttcagtt atcttagtag taggaattgg cggactcgtc tttgaagtag
    38281 gcacatttac attgtcggca atggcattag caactgtact tggcatcatt ttaaatctta
    38341 tcttaccaga aacttcacga agcgaagaaa actaaggagg aaacattatg atcatcaaat
    38401 cagaacgtat cagcttaaag catttattga ccgttgaggc acttactgat caagaagtta
    38461 tgggcttgat ccgcagaggg caggaattca agcaaggtgc taattggact ccgcaaaagc
    38521 gccagtactt tgctacaaac ctctttttcg aaaatagtac tcgcacacat aaaagcttcg
    38581 atgtagcgga gaaaaaacta gggctagaag tgattgaatt tgaagcaagc acaagctctg
    38641 tccaaaaagg ggaaacacta tacgatacag tactgacgat gtcagcatta ggcgtagacg
    38701 tagcggttat ccgtcatggt gatgagaact attatgacga actgatacaa agtaaaacaa
    38761 tccagtgttc gattatcaat ggaggagacg gaagcggaca gcatccaacc caatgcttgc
    38821 ttgatttgat gaccatttat gaagaatttg gtcattttga aggtctgaaa gttgccatcg
    38881 taggtgatat cactcactct cgcgtagcta aatccaatat gcagatgtta aaacgtctag
    38941 gcgcacaagt attcttctca ggtcctagag aatggtacga cgatgaattc gaagtgtacg
    39001 gacattatat gccattggat gaactactag atcaagttga tgtcatgatg atgcttcggg
    39061 tgcaacacga acgccatgat ggaaaagaaa gtttctcaaa agaaggctac catcaggaac
    39121 atgggttaac agttgagcgt gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag
    39181 caccagttaa ccgtgatgta gaattagctg attcattagt ggaaggatta cagtcgcgga
    39241 tcgttcagca aatgagcaat ggagtattcg tcagaatggc aatcttagaa gcagtgctaa
    39301 atggaaaagc gtaattatag gaggatgaaa aaatgaaaac actcattaaa aacggtcaga
    39361 tcaatacacg aaaaaatatg acgacaccag cagaaatttg gatagaagat gggcgaatca
    39421 aagcaatcgg tacaggattc tcagaagccg aatttgatga agtgtttgat gcaaagggac
    39481 aattgatcac acctggttta gtggacgtcc atgttcattt gagagagcca ggatttacat
    39541 acaaggagac gatcgaagct gggactcgct cagctgctcg tggcggtttc actactgtat
    39601 gcgcgatgcc gaatttaaat cctgtacctg atacggcaga aaagctgaga caagtatatg
    39661 acatcattag aaaagatgca gttgtgaagg tattgcagta tgcgccaatc acagaaaatc
    39721 tgcgaagtga gaaattagtt gatcaagaag cactgattga agaaggtgct tttgctttca
    39781 caaatgacgg tgtaggtgtc caaacagctg ggacgatgta tctggctatg aaagaagctg
    39841 caaaaaacaa taaagcccta gtagcccata cagaagatga atctttatta tttggcggcg
    39901 ttatgcacgc tggcaagaag gcagaagaac ttggtcttcc cgggatctta agcgtaaccg
    39961 agtcttctca aattgctaga gatctgttgc tagctgaagc aactggtgtt cattaccatg
    40021 tttgccatgt ttctactaaa gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa
    40081 tccacgtaac agcagaagtt tcaccccatc atcttatttt gatcgacgaa gatattcccg
    40141 aagatttcgg cttttggaaa atgaacccgc cgctaagagg acgggaggac agagaagctt
    40201 tgatcgaagg cttgcttgac ggtacgatcg attgcatcgc tacagaccat gccccccatg
    40261 gattagaaga aaaaagccaa agtttcatga aatcaccatt tggtatcgta ggaagcgaaa
    40321 cagcgtttca actgatctat actcattttg tagaaactgg tcgattcacg ttggaacaag
    40381 tgatcaattg gttggcagtt aaaccggctg agatttttgg tttgaatgca ggaacgctaa
    40441 cggttggtgc ccccgctgat gtagcagttt ttgatattac acaaacttgc acgatcgata
    40501 aagaagactt tttatcaaaa ggggagaata caccttttat cggctggaaa gtaaaaggcg
    40561 aaacacaaat gacttttgtc aacggaaaac ttgtgtggca gaagggagaa taaaagtatg
    40621 gaacgtttgc ttatattaga agatggaaca gttttcgaag gaaaggcttt tggtgcagat
    40681 gctagtgtct tcggagaaat cgtttttaca acaagtatga ctggttatca agaaactatc
    40741 actgatcaaa gttttaacgg tcaaatcatt acatttacct atcctatggt gggtaattat
    40801 ggggtaaata gagatgatta tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa
    40861 gaacatgcac gtgtagcttc aaactggcga aaccaaatga cgctagattc atttttgaaa
    40921 caaaaaggaa ttcctggaat atcaggtatc gatacgcgcg cccttactcg caaaatccgt
    40981 gaagtcggaa cgatgaaagc tagtatagtt gatgctggag attccttcga acatgcattt
    41041 gatcagctga aagcaaccgt tctagcaacc aatcaagtgc agcaagtctc aactaacaaa
    41101 ccttatccaa gtccaggaac aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat
    41161 agtattttac gagaattgag taaaagaaac tgtaacctca cagtacttcc ctataacaca
    41221 gatgccgaga cgattttagc gctatcacca gacggagtca tgctgacgaa tggacctggt
    41281 gatccaaaag atgtgccaga agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg
    41341 atttttggta tttgtctcgg tcaccagtta tttgctttag ccaacggtgc agatacctat
    41401 aaaatgaaat tcggacatcg aggattaaat cacccggtac gagaagtagc gaccggaaga
    41461 attgatttta cttctcaaaa tcatggctat gcagtcgatg aagccacgat tgatccagaa
    41521 aaactcatcg taactcatgt agaagtaaat gacggaacag tagaaggagt caaacacaga
    41581 gattatcccg ctttcagtgt gcagtttcat ccagatgctg cccctggtcc gcatgatgca
    41641 ctacatttat ttgatgaatt tatggaaatg atggacgcag gaaaggagca gtaaacagat
    41701 gccgaaacgt acagatatca agaaaattat ggtgattgga tctggtccga tcatcattgg
    41761 tcaagccgca gaatttgact acgcaggaac acaagcctgc cttgccttaa aagaagaagg
    41821 atatgaagtg gttttagtaa attctaatcc tgcgacgatc atgacggata aagaagtggc
    41881 agatcaagta tatatcgaac cgatcacact tgaatttgta tcaaggattc ttcgaaaaga
    41941 gcgtccagat gctctccttc caactttagg cggacagaca ggcctcaata tggcaatgga
    42001 gctggcagca tcaggtattt tagaagaatt gaacatagaa cttctaggaa caaagctttc
    42061 tgcgatcgat caagcagaag atcgtgatct attcaaacag ttgatgaaag aattgaatca
    42121 gccaatcccc gagtctgaaa tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa
    42181 aatcggctat cctgtcatcg ttcgtccagc ctttacttta ggcggaactg gtggaggtat
    42241 gtgtgaaaat gaagaagaat tacgtatcgt atcagaaaat ggattaagtc tttcccccgc
    42301 tacccaatgt ttgattgaac gaagtattgc aggttttaaa gaaattgaat atgaagtgat
    42361 gagagattcg gctgataatg caatcgttgt ctgcaatatg gaaaattttg acccggtggg
    42421 tatccataca ggtgattcta tcgtatttgc ccctagtcaa acattgtctg accatgaata
    42481 tcaaatgctt cgtgatgctt ctctttcgat tattcgagca cttaagatcg agggaggctg
    42541 taatgtccaa ctcgctcttg atccacatag ttttaattat tatgtgattg aagtcaatcc
    42601 gcgtgtttct cgttcatctg cgttagcaag taaggcgact ggctatccga tcgctaagct
    42661 agctgctaaa atagcgcttg gattaacatt agacgaaatg aaaaatcctg ttacaggaac
    42721 gacttttgca gaatttgaac ctgcattaga ttatgttgta gctaaaattc ctcgctggcc
    42781 gtttgataag tttgaaaacg gagaacggct tttaggcacg cagatgaaag caactggtga
    42841 agtgatggct atcggtcgga atatcgaaga gtctttattg aaagctgtgc gttctttaga
    42901 aatcggtaca caccatatcg aacttcctga gttggcagac atcacggacg atgacctaat
    42961 ccaaaaagtg gttcgagcac aagatgatcg cctgttttat gtagctgaag ctattagaag
    43021 agggtacacc atagaagaat tagcggattt gacaaaaatc gatttattct ttttagataa
    43081 actgttgcat atcgtagaaa tcgaacagca gttgaaacag cagccgttgg atgacaagat
    43141 attgaaaaca gcgaaacaaa acggttttac agacaaaaaa gtggcggagc tgtgggaaat
    43201 aaacgaagaa gccgttcgcc agatgagaaa agaaaatgat ttgattcctg tatataagat
    43261 ggttgacact tgtgcagcgg aattcgaatc aaaaacacct tatttttata gttcctatga
    43321 atatgaaaat gaaagttcac gatcagaaaa gccctcaatt ctagtactgg gatctggtcc
    43381 tatccgtatc ggacaagggg ttgaatttga ctatgcgact gtgcattctg tcaaagcaat
    43441 ccagcaggca ggatacgaag caatcatcat gaacagcaat cctgaaacag tttcgactga
    43501 cttttctatt tcggataaat tatattttga gccattgacc ttcgaagatg tgatgaatgt
    43561 gatcgagcta gaacagccga taggagtaat cgttcaattt ggtggtcaga cagcaatcaa
    43621 cttagccgaa cctttgactg ctgctggtgt aaaaatctta gggacaacga tcgaagactt
    43681 ggatcgtgcg gaaaatcgtg atttattcga gcaagcgctg aaagagttgg atattcctca
    43741 acctttaggg gatactgcta caagtaaaga agaagccgtg gttatcgctt cgagaattgg
    43801 ttatccagtt cttgttcgtc caagctatgt attaggcgga cgtgcgatgg aaatcgttga
    43861 aaaccagcga gatttagaag attacatgga acatgctgtg aaagcatctc cagaacatcc
    43921 agtactagtg gaccgttact tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg
    43981 tgaaacagta ctcatcccag ggatcatgga gcatattgag cgtgctggtg tccattcagg
    44041 ggattcaatg gctgtgtatc cacctcaaac attatctgaa tcgatcaaga aaacgatcga
    44101 agattacacg atccgcttag cattaggttt gaattgtgtc gggatgatga atatccagtt
    44161 tgtcatccat gaaaatgagg tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt
    44221 tccattctta agtaaggtga caggaattcc aatggcacaa attgcgacga aagctatttt
    44281 gggagagaaa ctaaagacac ttggttatga aaacggactt tatccagaaa caaaagaagt
    44341 tcatgttaaa gcacctgtct tttcttttac gaaattgcag aaagttgata cttacttagg
    44401 gcctgaaatg aaatcgactg gagaagtaat gggatctgac agacaattag acaaagcttt
    44461 gtacaaggca ttcgaagctt ctggattaca cttgcctgaa tatggagccg tattatttac
    44521 gattgcggac gaaacaaaag aagaagcact agaattagct aaacgtttta cagaaattgg
    44581 ctatagtctg ataggaacga aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt
    44641 tgaaacagta gcgaaaattt ctgaggaagc agcagaaaaa aatgtagtcg acttaataag
    44701 agatggtaaa acacaagtgg ttgtcaatac gattgataaa gatcggggaa atgcttctaa
    44761 agacggtttc atcatccgaa gagaagcagt tgaacatggt attccattgt ttacttcttt
    44821 agacacagca gatgcgatcg ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat
    44881 ttaacggcga gcacatgtta agacaaaaag acgaaaaacc gttgcaggag gggacagatg
    44941 aaacaagaaa tgatggaaat cgttcagcaa aaacagctgg caccaaagat ttttgaaatg
    45001 acattgaaag gtgatctagt aaaagaaatg cagcaaccag gacagttctt gcatattctc
    45061 gtgccgcgtg cagacttgct attacgccga ccgattagct tgaatcatat cgaccacgaa
    45121 acaaatactt gccgtatcat ttatcgggtg gaaggtgagg ggactagagt cttctctact
    45181 ctttctgccg gagaccattt agatgtcatg ggtcctttag gcaatggttt tgatttgtcc
    45241 aatcttcata caggagacga agtatttata atcggtggag gaatcggtgt ccctcctcta
    45301 tatgagttat caaaacagtt agtcgaaaaa ggaattaagc caacgcattt tttaggtttt
    45361 gccacacatg aagtcgttta ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc
    45421 gctactgacg atggaacata cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg
    45481 aagacggaac ctacagctgt ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa
    45541 cagctgtttt cttcgcatca aaatgtccag ttatcattag aatcccgcat ggcatgtggg
    45601 ataggggctt gctatgcttg cgtttgtcat acgccggaag atgaaacagg aacaaaaagt
    45661 gtgaaagttt gtgacgaagg accgattttt aaaataggag aggtaattat atgaatgaga
    45721 atcctttagc ggtcaaactt cctggcctag aattaaaaaa tcctatcatg ccggcaagcg
    45781 gctgtttcgg ttttggcaaa gaatacggaa aatactatga tttgaatcag cttggatcga
    45841 tcatggtcaa ggcaacgact ccaaatgccc gctttggcaa tcccacgcca agagtggcgg
    45901 aaacaccatc tggcatgttg aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta
    45961 tgcataaact tcttccagaa ttagaggagt acaatgagtt acccatcatc gcaaatgttg
    46021 ctggtgcttg tgaagaagac tatgtagaag tttgtagcaa aattggcgaa gcaccaaatg
    46081 tgaaagcaat cgagctgaac atttcttgcc caaatgtcaa acatggcgga attgcttttg
    46141 gaacagattc tgatgttgct tttcaattaa cacaagcagt aaagaaagtt tcttctgttc
    46201 ctgtctatgt caaattgtcc ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg
    46261 aagctggagg ggcagatggg tttacgatga tcaatactct tcttggtatg cggattgacc
    46321 ttaagacaag aaaaccgatt ttagccaatc aaacaggtgg actctccgga ccagcaatca
    46381 agcctgtcgc tattcgtttg attcaccaag tagcaagtat atccagcctt ccaatcattg
    46441 ggatgggcgg cgtgcagaca gtggatgatg ttcttgagat gtttatggct ggagcaagtg
    46501 ctgtagctat agggaccgcg aatttcaccg atccgtatat ctgtcctaaa ctaattaaag
    46561 agctccctgt gcgaatgagc gaactaggca tcgaatcgct ggaaagatta atcaaagaag
    46621 tgagagagga acgtgaacga tgaatcaact agaccaacga ccaattattg cattagattt
    46681 ttcaactagg caagaggtag aagactttct tcgatttttc ccgaaagaag aaaaattgtt
    46741 tgtgaaaatc ggaatggaat tgttctacca agaaggacct gaaatcgtac gttatctaaa
    46801 agaggctggt cataatgtat ttttagatct gaaacttcac gatattccga atacggtcga
    46861 aaaagcaatg cggggattgg caaagcttgg tgttgatctc acctgtgttc atgccgctgg
    46921 cggtattcgt atgatggagg cggcaatgcg aggattagaa gaggggactc cagaaggtgg
    46981 aaaacgtccg ctacttctag ctatcactca gcttacttct acaagtgaag aagaaatgca
    47041 tgccgatcaa ctaatcgaag tttctttaga aaaaagtgtg atccattatg ctagctgtgc
    47101 caaaaaagca ggattagatg gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga
    47161 aatggcagga gacgaatttg tttgcttaac tccgggtata agaccagaag gaacagtcgc
    47221 aggagatcag acaagagtag tcacaccttc tcaggcaaaa aagatcggct caacttttat
    47281 cgttataggt cgaccaatca ctcagtcaac taatccttat aaagcatatc aaacgattca
    47341 aacagaatgg agctaaccta aaatgaatgt tgaacaaagt atcgcaaaag acttattaga
    47401 aattgaagca gtatttttaa atccaagtaa tccttttaca tgggcaagtg gaatcaaaag
    47461 cccaatttac tgtgataacc gaatcacgat gagctatcca aaagtccgaa aagagatcgc
    47521 aaaaggatta gcaagcaaaa tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac
    47581 tgcaacagca ggaatccctc atgccgcatg ggtagcggaa atactggatt tgccaatggt
    47641 ctatattcga agtaaggcga aagatcatgg gaaaggtaac caaatcgaag gacgtatatt
    47701 tgaaggtcag aagatggttg tgatcgagga tttgatttct acaggcggaa gtgtcttgga
    47761 agcagctgaa gcagcaaaaa gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac
    47821 gtatgagttg ccaaaaggaa aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt
    47881 gaccaattat tctgtattga ttgaagcagc tttagaagat cgttatatcg atgagcaaga
    47941 attaactctg ctaaaagaat ggaaaaaaga tcctgaaaat tggcagattt aagcgcgctt
    48001 gatgaagata gagaaatccg atttgtgtct ccagaatcat caattggaat agcaaaaatc
    48061 caaaaactga ttgcgagaag catcttttca tcaggtatga aaagaaactt ctatcgtgat
    48121 cagtttttga tttttttaaa attcattttt ctaagactgg ttagcagaaa gaagagaata
    48181 atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa gggagctgtt
    48241 tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat atgatattcg
    48301 gaaaaagaaa tctcaaaggg aggtaacttt tatggggttg aatgaattta ttttaggatt
    48361 gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag agcatacagt
    48421 tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc
    48481 gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc atgactacac
    48541 agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg
    48601 gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag agattccagc
    48661 tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg
    48721 agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg atgaaatcat
    48781 caaaaataac ccggaaaaag tttataagga gatgttcatt gaggcagtga ctacgattaa
    48841 aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc cggaactatt
    48901 gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa
    48961 agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa agaatatgga
    49021 tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt tatgtgattg
    49081 gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca agggggaaga
    49141 aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt ttactgacaa
    49201 ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg tagagttcaa
    49261 gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac atatcatcaa
    49321 cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa aagagaatct
    49381 agttgtgggt gtcatgtacc agctaatggt acaggaaggt tggctgtata atcaagaaaa
    49441 tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg tgttctttcc
    49501 ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta ccagcgggcc
    49561 aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagagtttc ttctcccctt
    49621 cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg gaagagaaat
    49681 ttattatttc gaggagtttg agcaatgaat attcagcaga tgaaatacgt ggtcgctgta
    49741 gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca gccaagtctt
    49801 tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac tcgaacgaat
    49861 cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga gaaaatattg
    49921 gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga caatttttcg
    49981 atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact cgaggaattc
    50041 aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt gatcgaagaa
    50101 gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa tcaagctggt
    50161 atcagaagat atttacaaca aggagagcta agtggccgca gcattggaaa attcaaaacg
    50221 catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac caaagaagaa
    50281 ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc gtatttttat
    50341 gaagacctta tcgagatccc tgatcaagaa acagtgattt atacgagtga tcgaggtact
    50401 ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat tgtgattgga
    50461 gggattcgcg agcacttgcg gttaatccct ttggcagaag gcgaactaaa tgaattttat
    50521 atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat caaagagctt
    50581 actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt tattgaacac
    50641 cgttgatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt tttgtagcaa
    50701 acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta gaaacactca
    50761 ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc acgataatcg
    50821 tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt cggatatgct
    50881 ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa aaataggcag
    50941 ctaactcggc agcttctgta attgtttcat ctgagggctt gtcactttta atgatgacgt
    51001 gcgaaccggg tatatttttt gcatgaagcc aatagtcggt ttttttagct gtcttcattg
    51061 tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg tcagaagaaa
    51121 agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc ttctttttca
    51181 ggtatccttc tgaagtcaat tcttctttaa ttgcttcgat atccattggg ccggcaattt
    51241 ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct tggatttgtt
    51301 cgccaatcag cttaacagcg ttcttcagct tttggtatcg atggaaatat ttctgtgcat
    51361 tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct tcttcataat
    51421 aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta
    51481 gttcgccatt tctacggtaa ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt
    51541 ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc agttctccgc
    51601 cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct aataattgac
    51661 tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga taagcaatcg
    51721 gtgtgaaaaa atctttattt tccgtttcgt aaaaagtagg aataggctga tggctgatgg
    51781 ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt aatcttttgg
    51841 tcagctcttg agcgatatcg aaaccgattc cctgaaactg acgttggatt gcttttggat
    51901 ctagttcagt ttgggaaagc cgctgaaaga ttttttcttt ctcggagcta aatgggttca
    51961 agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa cgataagtgt
    52021 tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct cgattaacta
    52081 agacgatcgt actatgacgt cccattaatt caacgatcaa tatgatattt tgtagatcac
    52141 ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg atttgttcga
    52201 tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag ttaggcggat
    52261 tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt gggtgcgcag
    52321 ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt tcattttcat
    52381 aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat tcgttgacca
    52441 tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt ttcataagta
    52501 tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc
    52561 tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa atccaaattt
    52621 ttaggtataa taaaaaatag gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt
    52681 agttaaatcg agagtacaag aaatgaatga acatttctca gtgtcattcg cagaaaatcc
    52741 aagtaagtat tttcgacgtc ttgtttttct gctcatctta tcattgctgt taacagttgc
    52801 aatgttttta ttgccggaaa aaatccgaga agagcaagta aatagtctcg tttattcgtc
    52861 cataccaacc agcaaattga aaccttttga ttacagcaaa gctgatcaga aaatcaaaca
    52921 aagccgggcg atatcggtga tgttcagcaa accgaacggg aaaacctatc aggacatgct
    52981 tgatgttttt aatgatcctg agcttatgga agaattgaat cgaccgatct tttattatcc
    53041 gatcgtttat gatgtacatg aactggaaca aaaatataac atacgaacgg atgaagtgac
    53101 atttattttc tttgatggag gaaaagaagc aaatcggata actgcgggta agggaggaat
    53161 aactgatttt gataaggagt tgatccctga actgaatcga cttccgttag ccaatatcaa
    53221 acaattagaa gaagaattgg atagaacggg agtaacttcc caaaattccg agattgctaa
    53281 ttgagacatt ggcgcgatga aacggtgggt ctatatagcg ctatttttaa ttatcagaac
    53341 aagtgatgtt tgatcttcaa aataacaaat aaaaaattaa aaaatatagt tgacgaaatt
    53401 caaaaattcg attagactta aatcaattct taaggaaagg gtgacctgta atgaacttat
    53461 caaaacgtat ttcagcagaa aacaaaaaaa attggcgcag ctggcgtaga taagttgtat
    53521 taatggtgag actttcccat agatacaact ttttagagtg catttctaaa gcttgtatct
    53581 atgctagaca tgaacattga ctgccgcata gattagaatc tatgcggttt tttgtgtaaa
    53641 gacaatgacc gctttggcca ttgtcttttt ttgtagactg gatacttgcc tgattataat
    53701 aaataaaaag attgactttg caggaggaaa aaaatgaaaa acaaacgatt aataacagtt
    53761 gtagtaatta ttttagttta tttagcaggt gcatttattt atggaaaagc ctcaggcgaa
    53821 aaagaaagta cttcgacgaa ccagacaaaa aaaacaacag ttgttggggt attgcaatac
    53881 gtgagccatc cggcattaga tgagatttat cgaggaatca aagatggtct gaaagaagaa
    53941 ggattaaaag aaggagaaaa tcttgagatc aaatttcaaa atggacaagc cgatcagagc
    54001 aagctagcca caatgagtca acagctagta caatctgatc cagatgcctt gattggaatc
    54061 gcaacacctg ccgctcaatc attagcaaac acgacttcat cgatcccagt tgtgttaggt
    54121 gctgtaacgg atcctgtggg ggctggatta gtcgcttctt tagagaaacc tggtggtaat
    54181 gtaacaggtg tttctgatca gccgcctgtt acttcacaaa tcaagcttgc ttctgatttg
    54241 ttgccaaatg caaaaaaagt cggaatcttg tatgcttctt cagaggataa ttcaaaatac
    54301 caagtagcac aagcagaaga agctgcaaaa aaagaagggt tagaatcagt aaaatatgcg
    54361 gtgccttcca ctaatgagat tgcacaaacg gttcaagtga tgagccgaca agtcgatttt
    54421 atctatgttc cattagataa taccattgcc aatgcgatgc aaacagtggt aaaagaagca
    54481 aacaaagcaa atatccccgt cattccatct gtggatacaa tggtggaaca aggtggacta
    54541 gcaacaatcg ggatcaatca atatcaatta gggcttcaaa gcggtaagat ggcggcagaa
    54601 cttgcgagtg gaaaagaaaa accggaaacg acgcctgttt atacatttga tcaaggggat
    54661 acagtgatca atcaatctca agctgatcgc ttaggaatca ttgttcccca atcgatgaag
    54721 gaaaaagcca aaatcattac agatgactcg cagcaagaaa catctaagga ggatgacaaa
    54781 tgatcgtatc agctatttca caaggcatgc tttgggcaat attaggatta gggattttca
    54841 tgacttatcg gatcttagac tttcctgata tgacgacaga agggtccttc ccattaggtg
    54901 ctgctgtctg tgtgacagct attactcatg gggtagcgcc gattgtagct acattattag
    54961 gcgtaggtgc tggcatgata gcaggactcg ttacggggtt actttataca aaaggaaaga
    55021 taccggttat tttagcaggg attttagtga tgtcaggatt gaactcagtt attttgtttg
    55081 tgatgcgaac accaaatctt tctttattga atcatccgat tctacaagac agcttccatc
    55141 gctttgggtt acctgattac tttgacacag tccttcttgg cttcatctct ttgggcatct
    55201 tgattgctgt actgctattc ttcttcaata cagatttggg tcaaggctat attgcgacag
    55261 gagacaacga aacgatggct cggtcgttag ggataaaaac cgatcgtatg aaaattttag
    55321 gtttaacttt gtcgaacggt gtcattgcat tatccggtgc gctgattgcc cagaacgacg
    55381 gatatgcaga tgtaaataaa gggattggcg taatcgtcat cggtcttgct tccatcatca
    55441 tcggtgaagt gatcttccaa gaattgacgc tagcagaaag attgattgct attgttgtcg
    55501 gcagtatcat ttatcagcta ttgattttag tagtaatcaa actagggttt gacacgactt
    55561 atctgaagtt gttctccgct atcatcttag ctatttgtct gatgatcccg caattgaaga
    55621 aagcactgaa cttaaaaacg ggttttgaga aggaggcata gtaatgactg atatattgac
    55681 aatcaaaaat ggcagaaaag tcgtaaacaa tggaatgaat gaagagaaag tactattgga
    55741 tgaaattaac cttacaatca gagaagggga cttcatcact gtccttggcg ggaatggtgc
    55801 tggaaaaagc acactgttca atacgatcgc tggtacgctt caactcagtc aaggatcgat
    55861 ctcttttaaa gatcagctga tcacaaaaga ttcagaagaa aaaagagcac gattcctttc
    55921 tcgagttttc caagacccca agatgggaac ggctcctcgg atgacagtag cagaaaattt
    55981 gctacttgca caaaaaagag gtcaaaaacg gaaattccgt ttacgtaact tgaaaaatca
    56041 aaaagaagat tttttcagac tatgtcaaga agtcggtaat ggcctagagc aacacttgga
    56101 tactccagcg ggtgaattat ctggtggtca acgccaagca ttaagcttgc tgatggcgac
    56161 gatcacagct cctgagttac tattattaga tgaacatact gcagctttag atcctaaaac
    56221 agctaaagca ttaatgcgat taacaaatca gcggatcaat gaacaaaaat tgacttgtct
    56281 catgatcacg catcgaatgg aagacgcctt gaaatacgga aatcgtctga tcgtactcca
    56341 aaaagggaaa atcgttcaag atatttctga aaaagaaaag caacatttga caatgggcga
    56401 ccttttgcag ttctttgaag aagtagtgga ttgatttcga cagaaaaatt cctttcagta
    56461 gaaggacttg aaaactcatt gacatcttcc tttgattcat cctatactta aaacaataaa
    56521 caattaaaca tatttgggtg ttttgtagga tcacgtagtc aatgatttta acaattaaat
    56581 agaatattct ttgctctgac aagtaaacga tgagaaaaga atttgctact ttgccaccta
    56641 gacgtttcgt actttttcgt atcaaacgct aagtggcttt ttttattgaa gtaagaacaa
    56701 aggaggaata agatgatttc tggacaaacc aaattagctg ggctctttgc tgatccggct
    56761 tctcacagtt tatccccatt gatgcacaat actgcttttg aagcaaacaa catcgatgcg
    56821 gtgtatttag ctttccaagt aaatccaatg aatttgaagc aagcagtcga aagtattcga
    56881 acttttgata tgttaggtgt aaatctttcg atgccgaata aaacagcggt tattccttat
    56941 ctggatgagg taagccagga agcgcaattg atcggagcag tgaatacgat cgtgcacgaa
    57001 aagggtcgtt tgattggcta taatactgat ggtgcgggct tcatgcaatc cgtgcaagaa
    57061 gctgggattt cgatcaaaaa gaaaaaaatg actctactgg gagctggagg tgcagcaaag
    57121 gcagtcactg ttcaagcagc gttagacggc gctgaggaaa tcgtagttta taaacgaaac
    57181 aaccaaacat tcgctcaagt aaaagaaaca ttcgaacaac tcagctatcg aacttcttgt
    57241 aagataaaag ttcgtgactt tgccgatacg catcaattaa gaaaagactt gaaagaaagc
    57301 tgtcttctag tcaatgcaac agatatcggg atgggaaaga aaatcaatca gtcaccgctc
    57361 ttagatataa gcatgatgca tgagaatcta gcggtttttg atttgattta tgcacctaga
    57421 gaaacacgtt tattaaaaga agcaaaaaaa gtaggagcaa aaggatacaa tggacttggc
    57481 atgttgatct accaaggagc aatcgcgttt gaattatgga caaaggaaaa gatgccgatc
    57541 catgtgattg aaaagctgtt tgattaaaaa aggataatag ataaagatct tttattctcg
    57601 cataaacggt ctcaagaaaa gtaatccttt atgtatcgaa gcatttattt cgttattttt
    57661 tattaaatta tcaaaaaatg ataggctttt gtcgattggt tatcgtatcc tttatttatc
    57721 agtaagaaac gaggaggaac cacaatgagt cggatggatc gctataagga catccataag
    57781 aaggcgaaac ctctgaagcg ggatacgggg agtatttttg ctagaagaga acgaaaacga
    57841 acgcgtatgg aaaatgagga agatgctacc cgtatttatg aaagagagca gcattcattt
    57901 gaacatttga accaatacga gcaacagata tacagagatg caccaagagc agaagaacaa
    57961 caaaaaaata aaaaattttt caatcggcaa aagaagaaca aaccgttaaa acctcgaaga
    58021 aagcgatcgt ggaaaaaaat cattctatcg attttgcttg ttttgatcct ttattcaggt
    58081 atctcatttt ttattggtaa atatgtagct gaacatgaca caagcttaga ggcagcgact
    58141 tcagaaacct ttcatgggca aaaagcgacg aatggtgcgc ataatatttt gatccttgga
    58201 agtgatacac gcggagaaga tgccgggcga gccgatacga tcatggtctt acagttggat
    58261 ggtccttccc ataagccgaa actgatttct tttatgcgtg acagcttcgt tacgatccca
    58321 ggggtaggac aaaataagat caattcagct tatgcatacg gaggagccga tctagtgcgt
    58381 caaactttag tagaaaattt tggaatcgat tgtcaatact atgcgaaagt cgatttcaaa
    58441 tcgtttgaaa aagtgatcga tgccttattc atgaacggag tgaagatcga tgcagaaaaa
    58501 gatctgaatc tggatggtgt agatattaaa aaagggggcc agaagatgga tggacatgtt
    58561 cttttgcagt acgcacgttt ccgaatggat gaacaaggag actttggccg tgtcagacgc
    58621 caacagcaag taatgaacgc tattttcagc caattgaaga atccattgaa tctgatccgt
    58681 gcaccttatg cagctggtaa agctttagga tatacatcca cagatgtcac tgcttggttc
    58741 ttgacgaaaa acctcttttc gattgcacga ggagctggtg gcattgatcg aataagtgtg
    58801 ccagtagacg gttcgtggaa ttatgggcaa agtgattatg ctggcagtat tcttgaaatt
    58861 gataatcaaa tgaatcaatc tgctattaca gattttttga ataaatgatc ataaaaaaga
    58921 tcaagaagaa gctgcgcaag gcttttgtca tgaatcaatc aaacgattca ctcgtaaaaa
    58981 acgctttctt atggtagatt taaccaagag aggagctgtt tacattggat atctatctga
    59041 acttcaaaaa tgagtcgcgt gaagccatcg ctttttatga aaaagttttt cagactgaag
    59101 catcaggtat caccacatac ggtgaaatgc ctggaataga acaaatgaac ttgccagaag
    59161 aagcaaaaaa tctaatcacg aatgcacggc tgaacattta tgatacatta gttatgtttg
    59221 cagatacgcc agaaggaatt ggtcctgaat tagttgtggg aaataatgtt tctttagtca
    59281 tccagacaag aaatgaagaa gaaacgaagc gcctatttga aagcttagct gagggtggta
    59341 agattgagat gccactggat gctgttccgt gggctcagtt gtatgggatg gtaactgatc
    59401 gatttggcat caactggcag attaaccaag agtgacagct aaagatgaat attttctgga
    59461 aacatcaagc agcaaacgga tcatcgtttg tttgcttgat gtttttgtgt ctatttcgac
    59521 tatacggtat ctttttttta ttgccagatg cgaaaatatg ttccataata gagaagtccg
    59581 caaaaaatta agtaacggag gtcagaagat gaagacggtg atactagctg aaaaaccttc
    59641 acaagcaaaa gcctatgccg aaaccttcca acagcaaaag cggcatgaag ggtattatga
    59701 aatcagcgat ccattgtttg ctggtgaagt aaccatcact tatggttttg gacatttggt
    59761 agatatggtt gctccaggag cttatgaaga gcgttgggca aaatggtctt tagaaaattt
    59821 gcctattttc ccagaaacct tccgttacga agttccaatg gacaaacgtg cacaattttc
    59881 gatcgtaaaa cgagaattgc agtcagccga taccatcatt attgcaacag atggtgatcg
    59941 agaaggtgaa gccatcgcgt ggtcgattat tcgacaagca aacgcatttt ctaaggaaaa
    60001 gaactattta cgtttatgga tcaattctct tgaaaaagaa gcaatctatg aaggttttaa
    60061 gaatctccaa cctggaaaga actattttcc aaaatataaa gaagcgcaag caaggcagaa
    60121 cgctgattgg ctgattggaa tgaatggaag tccattatat agcttgctac ttcaacgaaa
    60181 agggattgat ggcagttttt ctcttggtcg tgtacaaaca ccgacactct atatgattta
    60241 tcagctgcaa gaagaaatca aacacttcaa aaaagaaacg tactttgaag gagaaggttt
    60301 aattacgaca aacaaagggc agttttctgg aaaaatcgta ccaaaaaaag catttaaaac
    60361 ccaagaagag ttaacgaagg aaatagaaaa acttggagcg catttaggta agcaaaacgg
    60421 acggattctt gaagttacga aaaaggaaaa gaggttgcat agtccacgtt tattttcgct
    60481 atctagtttg cagacaaaga tgaatcagct aatgaaagca agtgccaaag ctacacttga
    60541 tgctgctcaa ggattgtacg aaagcaagtt tctaagctat ccaagaacgg attcctttta
    60601 cattacagaa aatgagcatc agtatctggt taaacatctg caaaattata aaaaattttt
    60661 aggaatagaa gaagtacaga caactgagat caaagcaaag aaacgatatg tcgatgcgaa
    60721 aaaagttcaa gaacatcatg caattattcc gacaaagacg attccgactc ctgcacgctt
    60781 tgcgaagctt tccaaattac agcaagcaat ctatttgcaa gttttgcgca ctactgtggc
    60841 tatgtttgct gcagattata tctacgaaga gacgaccgta atgacgggcg tacagcaatt
    60901 aaagctaaaa agcatcggaa agatcccatt acagcaaggt tggcaggaaa tcttacgcat
    60961 caagtcaaaa aagaaagaag ctcagactct tcctcctatc gtagaaaatg aagaagcaga
    61021 agtagaactg aaaagtatgg agaaagaaac acaacctcca aaaccctata ccgaaggaac
    61081 gttgatcaca gcaatgaaaa ctgcgggaaa aacgctggat aatgatgaag cgcaagaaat
    61141 tctcaaagaa gtagaaggaa tagggacaga agctacacga gccaatatca ttgaaaatct
    61201 taagcagaag cattacatcg aagtcaataa aaacgaaata actgttacgc caaaaggaat
    61261 cactctttgt aaggcagtag ccaatgaacc attactgacg agcgcagaaa tgactgcgcg
    61321 ctgggaaggg tacttgagaa aaattggtaa acaagagggg acaccatctg tttttttaga
    61381 gaatatcaaa aaattcatcc tacatttatt ggcagaagtt cctaatcaaa tcgacaaggt
    61441 agacttttca gatgagatta gcgggacacg tcagataaaa gcgatggaga agaaaaacga
    61501 tcaattagga agatgtccaa aatgtaaaaa gggaatagtc atgctttatc ctaaaatcgc
    61561 tacatgcttg aatccggaat gcgacttcaa attgtggcca acagtagcta aaaagaagct
    61621 gactaaaacg aatctcagag atttgctttc aaaaggaaag acaagtaaag ttgtcaaagg
    61681 ttttactggt aaaaaaggga aattcgatgc agttttagta ttaaaagaag atatgacaat
    61741 cggtttttct tttccatgga tcgaaaacag tggaacaaaa gcagttgatg gaaacaaaga
    61801 aacatcaaga aaaaataatg aaaacgaagt agagtaaatg aaaacaatga aatagcggaa
    61861 tatttattcc gaaaagtgag aatataaata ccaataaaac taaaaaatat cagtgaatca
    61921 agcaaaaacg ttggggtagg ctgattttat atagatagca aggagttttt ttatttgaaa
    61981 aaagtgcatt catttttaaa ttatcttatg ggagcaatca cgattgtggc tgtgtaccaa
    62041 gcatttctaa agtatcctga ttacgggacg aagttgttgt cattgacgca caatcaaaca
    62101 gcatattcac caacgttgat tttatcgact ttgactgcag gagctaaaag cgctacttat
    62161 ttttttcata aaacgacaag catgctaaag cgaaagaaac agcgaaaaca attgttggtg
    62221 gtggtagcgg ctatgatcat tatttatttt ctaccagtta ttacacaagt atttcatcaa
    62281 tattttggtt aagaatacga gagtttgtga caaaagtcat gtcacaaact tttttctttg
    62341 tcattttttc gtaagaaaaa gcaaaataaa agtatataca ttgattaaaa gtatatactt
    62401 ggatataatg tacttactat agaatgagaa tggtatagag ggtggcgaaa tatgaaaaaa
    62461 atgaaaaagt ataaaattag aaaatctggc aatagcgatg taacaactat cccaccagaa
    62521 gtaaaagaat tcatgggtgt gcagacaggt gacgcaatca gttatgtttt tcaatctgat
    62581 ggttctgtac gaatgatcaa agcacaagaa gaacctgata ttgattcatt agttgattcg
    62641 atcatgaatc aatatgaaga cgcgttaaaa gatttggttg atttatgagg tatctttcga
    62701 ctaaagaaat tatcaaaatc aacgcgaaag ttattttacc gtcactcgcc aggcgaaatg
    62761 atcggtgtaa aagatgcaaa tgcattagat atggccgtca aacagccttc acaagcagta
    62821 ttcaatcaag aactctatcc tgaaatctat gaaaaagcgg cgattttagc aattaattta
    62881 gctaaaaaac atccttttca taatggcaat aaacgaactg ctcttgttgc catgttgttg
    62941 tttcttcaat tgaataattg tccagttgct ttttctcgtc aagaagctgt tgattttatc
    63001 ataatgatta ccactagttc attggatttc gattcactaa agtccaagat aaccagttat
    63061 ttacgacaaa ccgcaataaa atgaacgaat taaaaacttt atttctcaat tagatcagca
    63121 tgtcattaag gaatatgctg atttttttat gtttaatcat acaaaatagt tttttcataa
    63181 aaatatggaa aatatggatt aattttgaaa taattgattt aaaaggaata caaaagagtg
    63241 aatagtagga aatagtggtt tagaactagt gggctgattt tttatatatt ttagtttaca
    63301 taatatatat tatacaaagt agatttaaaa aatgataaac ctctctcgaa cggtttttta
    63361 tcaaaaatcg tcatttttcc atcaaaatct gcttatgcaa aat
//
LOCUS       DACECR010000004        57822 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.004, whole genome shotgun sequence.
ACCESSION   DACECR010000004 DACECR010000000
VERSION     DACECR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.004"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="IWO17_000206"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="IWO17_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0289110.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="IWO17_000207"
     CDS             853..1041
                     /locus_tag="IWO17_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0289111.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="IWO17_000208"
     CDS             1054..1611
                     /locus_tag="IWO17_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0289112.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="IWO17_000209"
     CDS             1643..1885
                     /locus_tag="IWO17_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0289113.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2226..3440
                     /locus_tag="IWO17_000210"
     CDS             2226..3440
                     /locus_tag="IWO17_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0289114.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            3471..4898
                     /locus_tag="IWO17_000211"
     CDS             3471..4898
                     /locus_tag="IWO17_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0289115.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            4921..5847
                     /gene="glsA"
                     /locus_tag="IWO17_000212"
     CDS             4921..5847
                     /gene="glsA"
                     /locus_tag="IWO17_000212"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ0289116.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6055..7515
                     /locus_tag="IWO17_000213"
     CDS             6055..7515
                     /locus_tag="IWO17_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289117.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            7736..9499
                     /locus_tag="IWO17_000214"
     CDS             7736..9499
                     /locus_tag="IWO17_000214"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ0289118.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            9583..10041
                     /locus_tag="IWO17_000215"
     CDS             9583..10041
                     /locus_tag="IWO17_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ0289119.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10150..10833
                     /locus_tag="IWO17_000216"
     CDS             10150..10833
                     /locus_tag="IWO17_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0289120.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            10980..11594
                     /locus_tag="IWO17_000217"
     CDS             10980..11594
                     /locus_tag="IWO17_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ0289121.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            11610..11816
                     /locus_tag="IWO17_000218"
     CDS             11610..11816
                     /locus_tag="IWO17_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289122.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            12355..12912
                     /locus_tag="IWO17_000219"
                     /pseudo
     CDS             12355..12912
                     /locus_tag="IWO17_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            12970..15009
                     /locus_tag="IWO17_000220"
     CDS             12970..15009
                     /locus_tag="IWO17_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ0289123.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(15118..16269)
                     /locus_tag="IWO17_000221"
     CDS             complement(15118..16269)
                     /locus_tag="IWO17_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0289124.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(16423..16953)
                     /locus_tag="IWO17_000222"
     CDS             complement(16423..16953)
                     /locus_tag="IWO17_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289125.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(17052..17402)
                     /locus_tag="IWO17_000223"
     CDS             complement(17052..17402)
                     /locus_tag="IWO17_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ0289126.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(17559..19088)
                     /gene="ahpF"
                     /locus_tag="IWO17_000224"
     CDS             complement(17559..19088)
                     /gene="ahpF"
                     /locus_tag="IWO17_000224"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ0289127.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(19098..19661)
                     /gene="ahpC"
                     /locus_tag="IWO17_000225"
     CDS             complement(19098..19661)
                     /gene="ahpC"
                     /locus_tag="IWO17_000225"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ0289128.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(19805..20467)
                     /locus_tag="IWO17_000226"
     CDS             complement(19805..20467)
                     /locus_tag="IWO17_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0289129.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            20651..22297
                     /locus_tag="IWO17_000227"
     CDS             20651..22297
                     /locus_tag="IWO17_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0289130.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            22288..23370
                     /gene="uxuA"
                     /locus_tag="IWO17_000228"
     CDS             22288..23370
                     /gene="uxuA"
                     /locus_tag="IWO17_000228"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0289131.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(23478..25031)
                     /locus_tag="IWO17_000229"
     CDS             complement(23478..25031)
                     /locus_tag="IWO17_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0289132.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            25179..26227
                     /locus_tag="IWO17_000230"
                     /pseudo
     CDS             25179..26227
                     /locus_tag="IWO17_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(26353..27432)
                     /locus_tag="IWO17_000231"
     CDS             complement(26353..27432)
                     /locus_tag="IWO17_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0289133.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(27535..27975)
                     /locus_tag="IWO17_000232"
     CDS             complement(27535..27975)
                     /locus_tag="IWO17_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0289134.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(28032..28442)
                     /locus_tag="IWO17_000233"
     CDS             complement(28032..28442)
                     /locus_tag="IWO17_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0289135.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(28622..29056)
                     /locus_tag="IWO17_000234"
     CDS             complement(28622..29056)
                     /locus_tag="IWO17_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0289136.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(29156..30004)
                     /locus_tag="IWO17_000235"
     CDS             complement(29156..30004)
                     /locus_tag="IWO17_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0289137.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            30393..32159
                     /locus_tag="IWO17_000236"
     CDS             30393..32159
                     /locus_tag="IWO17_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ0289138.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            32261..33520
                     /locus_tag="IWO17_000237"
     CDS             32261..33520
                     /locus_tag="IWO17_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0289139.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            33667..34968
                     /locus_tag="IWO17_000238"
     CDS             33667..34968
                     /locus_tag="IWO17_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0289140.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            34971..35822
                     /locus_tag="IWO17_000239"
     CDS             34971..35822
                     /locus_tag="IWO17_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0289141.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            35835..36650
                     /locus_tag="IWO17_000240"
     CDS             35835..36650
                     /locus_tag="IWO17_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ0289142.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            36801..37967
                     /locus_tag="IWO17_000241"
     CDS             36801..37967
                     /locus_tag="IWO17_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0289143.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            38197..38844
                     /locus_tag="IWO17_000242"
     CDS             38197..38844
                     /locus_tag="IWO17_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ0289144.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            38928..39872
                     /locus_tag="IWO17_000243"
     CDS             38928..39872
                     /locus_tag="IWO17_000243"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ0289145.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            40007..41458
                     /locus_tag="IWO17_000244"
     CDS             40007..41458
                     /locus_tag="IWO17_000244"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ0289146.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            41479..42315
                     /locus_tag="IWO17_000245"
     CDS             41479..42315
                     /locus_tag="IWO17_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0289147.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            42315..42974
                     /locus_tag="IWO17_000246"
     CDS             42315..42974
                     /locus_tag="IWO17_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0289148.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            43100..43789
                     /locus_tag="IWO17_000247"
     CDS             43100..43789
                     /locus_tag="IWO17_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289149.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            43795..44751
                     /locus_tag="IWO17_000248"
     CDS             43795..44751
                     /locus_tag="IWO17_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ0289150.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            44795..45307
                     /locus_tag="IWO17_000249"
     CDS             44795..45307
                     /locus_tag="IWO17_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0289151.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(45563..46111)
                     /locus_tag="IWO17_000250"
     CDS             complement(45563..46111)
                     /locus_tag="IWO17_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0289152.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(46200..47381)
                     /locus_tag="IWO17_000251"
     CDS             complement(46200..47381)
                     /locus_tag="IWO17_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ0289153.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(47617..49125)
                     /locus_tag="IWO17_000252"
     CDS             complement(47617..49125)
                     /locus_tag="IWO17_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ0289154.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            49284..49475
                     /locus_tag="IWO17_000253"
     CDS             49284..49475
                     /locus_tag="IWO17_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289155.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            49678..50595
                     /locus_tag="IWO17_000254"
     CDS             49678..50595
                     /locus_tag="IWO17_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ0289156.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            50588..51661
                     /locus_tag="IWO17_000255"
     CDS             50588..51661
                     /locus_tag="IWO17_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289157.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            51809..52327
                     /locus_tag="IWO17_000256"
     CDS             51809..52327
                     /locus_tag="IWO17_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289158.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            52308..52700
                     /locus_tag="IWO17_000257"
     CDS             52308..52700
                     /locus_tag="IWO17_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ0289159.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            52776..53711
                     /locus_tag="IWO17_000258"
     CDS             52776..53711
                     /locus_tag="IWO17_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ0289160.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            53844..54623
                     /locus_tag="IWO17_000259"
     CDS             53844..54623
                     /locus_tag="IWO17_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ0289161.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            54616..54933
                     /locus_tag="IWO17_000260"
     CDS             54616..54933
                     /locus_tag="IWO17_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0289162.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            55177..56787
                     /locus_tag="IWO17_000261"
     CDS             55177..56787
                     /locus_tag="IWO17_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0289163.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            56944..57792
                     /locus_tag="IWO17_000262"
     CDS             56944..57792
                     /locus_tag="IWO17_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0289164.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        19227 a        10208 c        11288 g        17099 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt tatatgaagt
     1981 attctttatt ccttatgaaa aaatatcttt aaaaataaaa aaagacgaaa ataaacggtt
     2041 ttctataaaa agaagaccgt ttattttttg tctttatttg tgtgaaaaac gaaaaagagc
     2101 acaacacatc aagcttttat taattgtctg tcttttataa atgcaagtat taattcaaaa
     2161 aaacagaaga aaaaaacaac tacaccttct tttatactcg taatcgtaga aaggaggcga
     2221 aaaaaatgaa tacattacct atcaatacag gagatacagc attcatgatt ttatgtacag
     2281 caatggtttg cctgatgact cctggtctag ctttttttta cggcggatta gcacggaaga
     2341 ataatatact aacgatcatg tctcaatctt ttctctccgt tgggataaca acgattatgt
     2401 gggtatttgg tggattcgga ttggcctttg gacgggatat cggcggtgtt attggaaacc
     2461 cggcagattt ctttttgatg agacatgtta caagcttccc caacatgttt catggtgcaa
     2521 cgattccatt tttaatgttc tttctttacc aactgatgtt tatcgtgatt accgttcctt
     2581 tgatgacagg agcttttgca gggagattga atttaaaagg ctatattata cttgttattt
     2641 tttggaatct gctgatttat ttccctgttt gccactggat atggggtggc ggatttttgg
     2701 atcagatggg atttagagat ttcgctggtg gagcagtcat ccacacgaca gctggattcg
     2761 gctctttagc atgtatcctc acacttggtc aaagaaagat tcctttagga gaaagaagtc
     2821 agcatagcag tcttatggca gctgctatcg gtacaggact gctttggttt ggctggttcg
     2881 gttttaattc cggtggtgca cttcgagcag atgaacaagc agtcaatgca tttggttcaa
     2941 cattcatcgc tcttgcattt gcgatgatta cttggctgat cattgcaaaa gtaaaaggaa
     3001 atggctttga ttttgtcgac gtactaacag gatccgttgc tggattggca gccattacgc
     3061 cttgtgccgg ttatgtagaa gcaaaaagtg ccatgctgat tgggatcatt gcgggtatcg
     3121 tctgtcatgc tgctgttgat tttcgtaaaa agaaacaatg ggatgatgca cttgatgtgt
     3181 ggggtgttca tggcatgggc ggattcacag gtacgattct gatagggatc tttgcatcta
     3241 acagccattt actaacgaat ccaagcagct ggtacttctt agggattcaa gtgattggtg
     3301 tcatcattac tgccgtgtat gcctatgtgc tgacaacagt gattttaaaa tcggcgatgc
     3361 actttacaac gatcactact acaaaagaag aacaagaaga aggattggac tttttcttct
     3421 ttggcaacaa actcaattga aacaaattca aatagaaaag aggaagattc atgcaacaaa
     3481 gcaaaacgaa aattcctgca aaaaaattaa cgttctttgg ctttttggct atgaccatct
     3541 caatggtcgt ttcattatat gaatatccca cattcgcgac ctcaggcttc agcttagtat
     3601 ttttcttgtt attaggcggt ttgctatggt ttatccctgt ggcattgtgc gcagcagaga
     3661 tggcaacggt gaaaggatgg gaaaaaggcg gtgtctatac ttgggtcagt cgcacattgg
     3721 gaaaacgatt cggttttgcc gcaatctttt tccaatggtt tgagataaca gttggctact
     3781 tgaccatgct gtatttctta actggtgcat tatcctatgc aacgggtatt ggtgctatac
     3841 aaaacaataa atatctgaaa ttggcaatcc tattgattat tttctgggct attttactct
     3901 cacagttaaa aggaaccaaa tacacttcat tgattgctcg gatcggtttt gtagctggta
     3961 tcttgttgcc agcattagtc ttgtttggct tagggatcca ttatgtcgct tcaggcgctc
     4021 cactacaaac gatactgtct atgaaaacat tgattcctga tttttcgaaa ttgcctacgc
     4081 tggttgtttt tgtttccttt attttagcat atatgggcgt agaaacatct gccagtcatg
     4141 caaatgaaat ggaaaaccct aaaaaggatt atccattagc aatgtttgta ttggttattc
     4201 tggcaatcat tttagatact tttggcggat tgaccgtcgc tactacagta cctcaacatg
     4261 gtttgtctct taatacaggg gtgattcaat cgttagaatt cctattaaga cacattgatc
     4321 caaatcttgc atggattgcg aaaattcttg cgattcttgt ttgtttaggt gtcatcggag
     4381 aaattgcttc ttgggtaacc tctccttcaa aagcacttca cgtagcagca gaagaaggat
     4441 tacttcctga atattttgca aaagaaaaca cacacggtgt tcctgctcat ttgatgatcg
     4501 caaatgggat cgttgcaaca atctgggcag ccgtctttac attaagcggc ggggggaata
     4561 atctttcatt tcttgcagcg atgtcattga ctgttgtaat ctacctgatg ttgtacttta
     4621 tgtttttcgc gggctatttg aagctggttt ttaaagatga cagtcttgaa agagcctatc
     4681 aggttccagg cggaaaagtt gtaaaagtaa tcgttgcttg tttaggtctt ttgacttcac
     4741 tttttgcttt ttgtatctca tttgttccac ctgctagtat cgcagctgga cagcatggga
     4801 tttatgaaac gattctgatt gtttcttatc ttatcgtctt agttattcca tttacgatct
     4861 acgcatgccg tcataaattc cagcatgcag aaaaataaaa aatgaaagct ggttattcaa
     4921 atggaagtaa aagaattaga acaattgatt ggaaaaaatt tccactatta tcaaacagga
     4981 caagtggcgg attacattcc agcattagct gaagtggatc ccaaacaatt aggtatggcg
     5041 atttatgatt tggatacaaa acaactgatt gagggaggag acgccgatgt gcgctttgcc
     5101 atcgaaagca tgtcaaaagt tcccgtattg ttattagcaa tcaaagacaa cggaatagaa
     5161 aaagtctttc aaacaatcaa cacggaacca accggttttg ccttcaattc tatcatgaat
     5221 atggaaatca atcatcgcaa acatccaatg aatccttttg ttaatgcagg agcgattgca
     5281 acaacctcgt tgattgccgg taaagatgca gatgaaaaat ttgagcgaat cttgtcattt
     5341 atgaaagaga tctgtgatga tcctaatatc acattgaatg aagaaatcta tcattctgaa
     5401 tcaagaacag gtgatatcaa tcgctctctt gcttactata tgaaaggtaa tcaaatgatc
     5461 gaaggagacg taccggagat actcgatgct tatttcagac aatgctccct caatgtcacc
     5521 gctacaggca tagcaaaatt agctgctgtg ctggcaaata aaggaattgc tccttggaat
     5581 ggcaaacaaa tcattactga agaaagtgct acaatcgtca aatcaattat gacaactgct
     5641 ggtttgtatg atgaatcagg agagttttcc gtccatgttg gtgtacctgc aaaaagcggt
     5701 gtaggcggcg ggttgatggc agctgtgcca aatcgttacg gcataggtgt cttcagcccg
     5761 gcactagatc catttggtaa cagcgcagct ggtatccaat tgttgaaaga tgtagtcaaa
     5821 gaactagacg ctgacatctt tgaataacca ttcttgaaag aggctggggt aaaagtccaa
     5881 acggctcaaa gctacctaca atgaggctga aattgtgagc ttgtttgact tttgtcccag
     5941 tttctttttt tattggtttt tgaaagggct tttctaaata aaaaggaaac aaaaatacga
     6001 tttacagata tgattaaata aaatcataag aaaaactgga ggcgtgtatc aggaatgctg
     6061 gaaaattaca tcgagcgaaa tatatttaga aaagtgtatc tgtgtgaaca attatttgaa
     6121 ttccaagaaa tagacatcga acaaacagct atttctctaa gggtgactac accaacgatc
     6181 cttcacgatc tagaaagtct agccgagtgt ctagaatatt gtataaaaga acaagtaaga
     6241 gagaagcata agtataaact cgtattcaaa catggtattg ctctttctga gctcacacag
     6301 tttctgtacg gtcaatctta ttttttaaag tttttaagtt attattttaa tggtatattc
     6361 acgagtacag aactggctga tcttgaattt atttctttga gcaaagtata tacaatcaaa
     6421 aaaatcgtat tggatttttt taaagaaaat gattatttga aaaataaaga aatcattatc
     6481 ccagaatttg actcgcggaa catcttgcta gctttagtac gttatgtcaa ttgggaaggc
     6541 tatgaaaata aaaatccgta tgtagaagaa gcttgtaacg agttgattga ttatgtagag
     6601 actcattttt ttaaaagaag atattcggaa gatgaaaagt cctttatcat acgaggaata
     6661 gagatcgcaa tcggaagaaa ggcatttccc cttggactga aagaagaaga taagaaaagc
     6721 gcagaaacga agccgttatt tcaattagtc tgtcaaggat tggaagagca aaaaggaatt
     6781 ctagacttac aagaagaaga tgtctattat attttttctt tgttcaacag tagaaattat
     6841 accaatgaga acatggagct gttaagaaaa gatttcgaag ttgtttataa aaattttgtt
     6901 ctcaatgacc tatcactgaa tgaattgatt ttacagatta ttagtatcgc tgaaatcgac
     6961 gatgcagaaa attttatttt caagcaatcc tttttacaat ttgtccgtac gttgtgggca
     7021 gatggacaag tatttttacc ggaaaggatc tatcttctat cagaaaaaga acaacttctg
     7081 tacggaaaaa ttgtagaaat attggaagag tggaaaaaga aaaatcaaat ccaactaaga
     7141 tggaatgaaa atttcattcg aaaattcgtc aaaagtcttt ctttgatcaa cagtgaacaa
     7201 gcggggagac aggaaataga aatttttgtt gtttcagaaa gttcagtcaa acaatttttt
     7261 tatcgaaccc aatttcattt gtatgcggaa gaaggcatcg ctgaaataaa tcctatttta
     7321 tttcgttcat tagaagaatt gcctgatgaa tgtctatgtg cgaaaaaaag agtggttttg
     7381 tgcgatgctg ctagctatca aaatggatta gaaactgaaa agacaaagat ctatccaatc
     7441 tcattaaaag gtgcaaacat ccatttgtat catattgctc aggaacttca cctattaaaa
     7501 aaacatattt cttgatgaga ccttaattag gagaccttct acaaagcttt ttaattgtct
     7561 gtagaaggtt tttatttgag tcctgattcg tgcgaagatc tacttatata acaagctttc
     7621 tctgaaattt ttttaaaatg ctattgactt agagtcaact ctaagtagta gaatagataa
     7681 agtgtaacaa atctattttc attaaaagaa gatagtacgg aggagagaat gtattatgag
     7741 tacagtatta catgtattgg atgcaatcaa tagccccgca gatgtcaaaa aattatctgt
     7801 tgaggaaatg aaacagttgg cagaagagat gcgccaaaaa atcttgcaaa aggacagcga
     7861 tgtaggtggg cacgttggtc cgaatctagg tgtagtagaa ttaacgattg cgtttcatta
     7921 tgtatttgat tcaccaaagg ataaaatcgt ttgggatgtt tcccatcagt cttatggtca
     7981 taagctttta actggtcgaa aaaatggttt tgaaaaagga cattaccgtg aagtgagcgg
     8041 atattccaac caacatgaaa gtaaacatga ctattttaca gtggggcaca catctacatc
     8101 tatcagctta gcaacaggtt tagctaaagc gagagatctg aagaaagaat caggaaatgt
     8161 ggtagcattt atcggtgacg gctccctaag tggtggctta gcttttgaag gattgaacaa
     8221 tggtggtgtt ttacaatcca atctaatcgt attagtaaac gataatgaga tgtctattga
     8281 tgagaatcat ggcagtttat ataaaaactt agctgagctt cgtcaaacca acggtaaaag
     8341 cagcaataac ctatttaaag caatgggatt tgattatcgt tacgtggata atggaaacga
     8401 tctggaaacg gtgatccgtt tatttgaaga agtaaaagat attgatcatc cgattgtact
     8461 tcacgtccat acggaaaaag gtcgcggtta tcagccggca atcgataata aaatgaaata
     8521 tcattggcat gtaccatttg atctgtccac cggggaggca aagcaagtaa cttctggaga
     8581 aacatttaat atgatcatca tggattattt ggataagaaa atgcaaacaa gtacagctcc
     8641 tattgccgca gtcaatgcag cgataccagg cgcctttgga ctgaaaacat tccaagaaaa
     8701 acatccagat cattattttg acgtggggat cgctgaacag cactctatca ctttcgcttc
     8761 tgctttggct tctcagggag tacgtccagt cgtcttccaa tcaagtactt tcttgcaacg
     8821 ggcatatgat caattatcac atgatttagc gatcaacgag aatccagtga ttattttagt
     8881 cggtggcgga acgatctcag ctagtgaccg aacacaccaa ggaacatttg acattcctta
     8941 cttatcaagt atcccgaaca tcaaatatct tgctccagca acaaaagagg atatgatctc
     9001 tatgctagat tgggcgttcg aacaaacagc gcagcctgta gcaatccgaa ttcctacaca
     9061 tggtgtagaa catggtacag ctatcgcaaa tgattatagt attccagcct atcaaacagt
     9121 aaataatgga ggaaaagtag caattttagc tttaggcggt tttcttgaat taggaaaaga
     9181 aacagtaaaa gcttttagaa aaaaaggaat cgaagcaacg ttgatcaatc caggaacgat
     9241 cacagaaaat gacgtagaaa cattagataa attaaaagaa acacacgaat tagtcatcac
     9301 attagaagat ggtagtttgt caggaggatt tggcgaaaat atcagtcgtt attatggtga
     9361 ttcttcaatg aacgtattaa attttggtgc agagaaagaa ttcacggata gcgttcctgt
     9421 agggcagctt tatgaacgtt atcatttaac accaaaacaa atcgttgaag atagtctgcg
     9481 tgtcttgaat attcaataat tcattatgct caccatccag ataattgttt atactagtat
     9541 ctggatggtt ttttcattat aaaaagcaaa ggggtggatg ttatgtatca gcaagtagaa
     9601 gaatatgtca aaaagttcca agaaaaatat cctagttatc aatcagaaaa attaggtttc
     9661 ttttcatttg gggatacaga aaaaatggca gacgagttga gcgaactagt tgtaaaaaag
     9721 ataaagactg gtacatcaag tggttacgat ctgtataaac aagagaatga gcctttacca
     9781 aaagttggtc aattagacgt ggtcctgaat ggaaaaaatg aaccagtttg tatcatcaaa
     9841 aatataaatg tgaccatctt gccttttagt gaagtcacga aagagcatgc ttggaaagaa
     9901 ggagaagggg atcggacatt agcttattgg cggaaagtcc atgctgagtt ttttaaacat
     9961 gcttataaag aagcgttgaa catagaattt acagaagaga agctggtcgt atacgaagaa
    10021 tttgaagtaa ttttctttta gtaagaaagg tctgctgaaa gaaatagctc attattgtct
    10081 cctgaataga aaagggttac atttttatat gaagctgtat ttttatataa cattactgga
    10141 ggagatattg tggatacact ggaacaaatc aaaggcggag tcgtcgtttc ttgtcaagct
    10201 ttggaaaacg agccgctaca cagttctttt attatgggga gaatggcttt ggctgcaaaa
    10261 gaaggcggag cagttggtat tcgagcaaat acgacagaag atatcaatga aatcaagcga
    10321 atcgttgatt taccggtaat agggattatc aaaagagatt acgatgggtc agaagtgttt
    10381 attactgcga cagaaaaaga agtggaagaa cttttggaga cgcaagcaga aatcattgca
    10441 ctggatgcaa cttctcgtaa acgtccgaca gaaaaaacaa ctgctgaact tgtccaaatg
    10501 attcacgaac acggtcggtt agctatggca gatatttcga cttttgagga agcgcttcaa
    10561 gcgcaagaag atggttttga tcttttgtct acgactttag caggctatac agaatattca
    10621 cgaaagacgc agacacctga ttttgattta ttgaatcaaa tcatagaaca agtcgatatc
    10681 ccagtaatta tggaaggaca tactgactct cctgaacaag tagagaaagc atatgaatta
    10741 ggtgcttttt cagtcgtagt tggatctatt atcacccgtc cgcaattgat cactaaaaga
    10801 tatgtcgaag ccgcaagtaa agcaaataac taaaaaacac gcacggcaca gattcttgac
    10861 aaaaaagaaa aattgataga tagtttaaag cagattctaa gaagatgaaa aaagcattac
    10921 ttttcatttt gtcttcaaga tctgcttttt aacgaatttt atagaaagaa ggcgaaacca
    10981 tgtttgaacg gagtccttca gaattgacag aacgtgaaaa ttacaagttg ctgattggtt
    11041 cagtcatccc tagaccagta gcagtagtaa cgactaaatc agcaaaagat atagtgaata
    11101 tcgcaccctt cagttacttc aatatcgtca gttcgaatcg acccatctta tccttggcga
    11161 tccagagaaa acaagatgca gtaaaagaca cagcaaagaa tattcttgag acaagagaag
    11221 ctgttatcca tatccttgat gaggacaacg tagaagaagt aaataaaaca gcagccaatc
    11281 tgcctaatga ggaaagcgaa ctgtacctga ctgacttgac tttaactgat tcagtaatca
    11341 tttcagtacc agcattgaca gaagcaaaag ttcgttttga aacatcctta tatgaacatg
    11401 tcgagatacg gaataaaaat aaagtaacag ctgatttatt tttgctagaa gtacagaaat
    11461 atcatttaaa tgaaacagtc tatgatgata agacaggaag gatcgatcca agaaaattga
    11521 atgctgtaag ccgtttggca ggaaatgatt atgcagcaat aggagaaatt ttcacgattg
    11581 cacgtcccga ataaaaagga gcactagtta tgttaaaaat aagctataaa ccatcgacag
    11641 attcaaaaga aatgaaaaaa gaatatgaaa cagtgaatga ttttctgcaa gggcagtact
    11701 tagaagttcc gccattgcaa gaccattttg tcgttactac tgttacttta gatggtaagg
    11761 aaatagaaat gccagatcaa acgattagcg gattgtttaa ttactttaac aaataaatat
    11821 ttattccgaa aagtgaaaaa acatataaaa aaacacataa aactaaaaaa ttatcaaaga
    11881 atcctggtgc aactgaaaga atttgcaacg ggattctttc aacttatttg ttattttttt
    11941 ctaaaaaata ttaaagattg ttaaatttca ctatttcatt tcaaattcca ttgcctaatc
    12001 attaggaaaa gtctatcatg aactcgtaaa tagaatatag taataacctt gttaggtgag
    12061 gctcctatac aaacataggc tgttgcccag aaacgtcgaa agtcgccaat gggtaaaaca
    12121 gaaactgttg gattaaggct ttttccaata gctaagaatg ttttctttac gttgtatagt
    12181 gctaaaactc ggacgatgag atgatgctag gtcagaattc ttgttcgaaa aggtttctga
    12241 ccttttcttt atatttttgt gactgactta tttgatgaga caaactcgct cctcacctga
    12301 caagtggatt gctgtgtgag gaggagatgc ggtgaaaaaa gatcgattgg tggaggtcgc
    12361 tcggattcac ttctggttgc acaatacaac cagaaaacac aacaagcaaa actgatctct
    12421 atcatgagag attcatatgt cgatattcca ggttacggaa tgaataagat aaatgcagct
    12481 tactcttatg ggggagttga tttattaaat caaacattaa aggaaaattt caaatttgaa
    12541 gctccctatt atgcaagtat cacatttcaa gattttatcg attgcgttaa tgaactgttt
    12601 cctggtggag tcaagatcga tgcagaaaaa tctctagatt tagatggcgt atatataaac
    12661 aaaggagagc aagtgatgga tggtaatact ttactccagt atgctcgatt tcgggaagac
    12721 aaagaaggag acttcgggag gatcagaagg cagcagcaag tgatcaaagc tatctctcag
    12781 cagctgaaag acgtaacttc aatattcaag ctacccaaag cggttgggaa attacttggt
    12841 agtatccaaa cgaaccttcc agaaagtgtg cttctggatt gtggtatgga tttcctcaaa
    12901 gacatatgtt agaataaaca ggtataaata gtgaaataaa ggaatgacaa gcaagagaag
    12961 gaggaaaaaa tgaaaagaag tgacaagcac ggcaaaaatc gaacaggcgc ttatattgcc
    13021 ggcgcagtga tcttaatagc agctgcgggt ggcggttatt tttactacca gcactatcaa
    13081 gaaacccaag cagtagaagc tggagaaaag acagttgagc aatttgtcca agctttgaac
    13141 aagggagatt ataacaaagc tgcagaaatg acatcaaaaa aggcggcaaa taaaagtgca
    13201 ttatctgaaa aagagatctt agatcaatac cagaatatat acggtgctgc tgatgtcaaa
    13261 ggacttcaga tatcgaatct aaaagtagat aaaaaagacg attctactta cagtttttca
    13321 tataaagcaa agatgaatac ctcattaggt gaattgaaag atctttctta taaaggaaca
    13381 ttagacagaa atgatggtca aaccacgatc aactggcaac ccaacttggt ttttccagaa
    13441 atggaaggaa atgacaaagt aagcctgacc acacaagaag cagcaagagg gaatattata
    13501 gatcgaaatg gtgaaccatt agcaacaaca ggcaaactaa aacaattagg agtcgttcca
    13561 agtaaacttg gagatggagg cgaaaaaaca gccaatatca aagcgattgc ttcctccttc
    13621 gacttaacag aagatgctat caatcaggcg atttcacaaa gctgggtaca acccgattac
    13681 tttgtcccat tgaaaatcat tgatggagca acgccagaac ttccagctgg agctaccatt
    13741 caagaagtag acggcagata ttatcctttg ggtgaagcag ctgctcagct gattggttac
    13801 gtgggagata tcacagcaga agatattgat aagaatccag aattaagcag taatggaaaa
    13861 atcggtcgct caggtttgga gatggctttt gataaggatc ttcgtgggac aacaggtggg
    13921 aaattaagca ttacagatgc agacggtgta gagaaaaaag ttctgatcga acatgaagtt
    13981 caaaatggca aagatatcaa attgacaatc gatgcgaagg cacaaaaaac agcttttgac
    14041 agtctaggag gaaaagctgg atcaactgtt gcgacaacgc caaaaaccgg tgatcttctt
    14101 gcgcttgcta gctctccaag ctatgatcca aacaaaatga caaacgggat ctcacaagaa
    14161 gattacaaag cttatgaaga aaatcctgaa catccattca tcagccgatt tgcgacaggt
    14221 tatgctcctg gatctacgtt taaaataatt acagcagcaa tcggtctcga caacggcact
    14281 atcgatccaa atgaagtgtt gacgatcaac gggcttaaat ggcaaaaaga cagttcttgg
    14341 ggatctttta aaacaacgcg tgtcagtagt gatgtatcac aagtagactt aaaaactgct
    14401 ttgatctatt ccgataatat atatgcggca caagaaacgt tgaaaatggg tgagaaaaaa
    14461 tttcgtacag gcttagataa attcattttt ggtgaagacc ttgatttgcc aattagtatg
    14521 aatccagcac aaatttctaa tgaagatagc tttaactcag atatcttgct agctgatact
    14581 ggatatggac agggcgaact tctaattaat cctatccagc aagcagcaat gtattctgtt
    14641 tttaccaaca atggcacact tgtctatcct aaattgattg cagataaaga gacaaaagat
    14701 aagaagaatg tcatcagcga aacagcagta caaacgatcg tgccagatct gagagaagtt
    14761 gtgcaagatg taaatggtac agcacattct ctttctgctt tagggattcc gttggcagcg
    14821 aaaactggta cagcggaaat caaagaaaaa caggatgtaa aggggcaaga gaacagtttc
    14881 ttgtttgctt tcaatcttga taaccaaggt tatatgatgg tcagcatgtt ggaaaataaa
    14941 gaagatgatg attcagcaac caaacgagca tcggaactat tgcaatacct caaccaaaat
    15001 tatcaataaa aaattgcttt ttaagtacag taaaaggact gtggcgttat cgtcacagtc
    15061 cttttttcaa ataaacggtt ttcacgatgt ctctttcaca acttcttttt gatttactca
    15121 cacttcataa actttttttg taaaatattt gagaacaagt ggagaaataa gtgtacttaa
    15181 taaaatcacg ataactaaag gtgaatagta ctgtggttcg atcaattgac tttgctgtcc
    15241 aatttgtaaa atgatcaaag ccatttctcc acgcgaaatc ataccagcac cgaccatcaa
    15301 tgaactatgt tgagaaaaac cggcaatctg agcaccaaaa taacctccaa gcaatttggt
    15361 caaaatcgct acaagcgtca atatagaaat aaagagtagt tgttcactaa agtcagaaaa
    15421 atcaacttct aatcctacac tgacaaaaaa tactggtata aatactgcat atcctaaagc
    15481 ctctacattg tggtatactt cttctttaac ctttgtctga ctgacagcta tcccagcaaa
    15541 aaaagcacca atgacagagc taagcccaac aagatccgct acgtaagaca ttcctaaaca
    15601 aataactagc gatacgatga taatagaaga atttgcgtac actttttctg caaatgaaag
    15661 taggaaaggc gcaatccatt tgactaataa gaaaataaaa acaaaataaa ttagctgttc
    15721 aatcaaaaca agcggtaaag aagcattgct agaagcttcc cccgttagaa aagataggct
    15781 gaaacttaga actaaaacga ctaaaatgtc atctaccaca gaagcaccca aaattgttga
    15841 tccttctttt gtattcacta cgtttagttc ttttaatact tctactgaaa tactgacaga
    15901 agttgccgca agaataatcc caaagaaaat cgcttcattt cccgaaactt ggaatgcttt
    15961 gcctgtcaac cagccaaaaa caaccgggaa gatgatccca agcaaagcaa cgaacatacc
    16021 aggtcggaaa tattttttca aaagagataa atcactttcc agaccagcta aaaacattaa
    16081 taagatcaca ccaatctccg aaaagtcatg gactagaata tcaggtttga cccaattcaa
    16141 tccagcttgg cctagcaaaa caccaacaag taattgtcca atcactgctg ggatacctaa
    16201 tctccgcgag atatgtgagc caatcgttgt tgaaatcaat atcaggcata aaataccaat
    16261 aaattccatt ctcatcattc ctttgtataa aatatagccc gaacttagca taacatacat
    16321 tattgaaaaa ataggaaaat atactcacaa aaaaaccaac aatccaaaag atttttttgg
    16381 attgttggtt tttatacggt ctattttaat aactaaaaat gattaacgta tagtcatttt
    16441 tcttattctg atgaaattga taatctcctg gatgcccatg aaaatcaaaa taaagccgaa
    16501 tatttggaat agaagcatca aacttttgaa tggattgaac aataagacga atccgccaat
    16561 caacaacaga atactactat aaacactcca acttttagct tttgtattca tgctgaagaa
    16621 caactggaac aagctgttga tgataacagc taatccaaga agaatcggca ggatagaaac
    16681 gattgcaggt gcaaaagcat agacgatcag agctaagatc aggaaaaaaa gaccactgaa
    16741 cagaccgata ctccaagaac ctgttttcct tttaaaacgg taagcctcaa aaagattcaa
    16801 caacccaagc aaaacaagat aggcggtgat cagataaccg ataaaatgaa aaacagcaga
    16861 agggtttagc acaatcgcaa tcccagaaat aatataaaca gcagctctta ataaggcgta
    16921 tttttgaata ttttgtttaa tcgtctcaaa catataagaa aacctccttt gacttatttt
    16981 aacgtgaact agtcatttta ccaaattaaa tgttacaaaa tgagcgaagg agaagaatat
    17041 ctctggaaaa atcaacttct gaggatcttt tccatttttt tgccttttgc tagctcatcg
    17101 atcattttat ctaaataacg aatttctttc atcaagttat cctcgatgtc ttctactcgt
    17161 acaccacaaa caacacctgt aatcaaggaa cgtgaaggat tggggacagg ggcttccgca
    17221 aaaaattgtt cgaaagtcac ttgtttttgt agttgatatt ctagttctgt ttgggaataa
    17281 cccgtcagcc aacaaatgat ttcatctact tcctctttta tacgatcttt tctctccgct
    17341 tttgctacat acataggata aacgcttgca aaacttgtcg taaaaatacg attttgcgcc
    17401 ataatcagtc acctttcttt ttttcaaatt atttactact tatagtatat aagaaaaaga
    17461 gaagaaaaag cgattattca aaaaaacaaa aaggattgtg acaaaattct tgtcaccatc
    17521 cttttttccg ctctctgact ttttacaatc ttcatagctc aagtgcgcat cagataatcg
    17581 aatgctccta aagctgcagt agctcctgaa cccattgaaa caatgatttg tttgtatgga
    17641 ctatctgtac aatcaccagc tgcgaaaacg ccggggatat tcgttgcgcc atttttctcg
    17701 gtcactattt catttcttgg attcatttcg atcgttccat ctagccaatc cgtattaggg
    17761 agtaagccga ctagaatgaa tactcctgaa acagctagtg tatgcatctc catcgtacgg
    17821 cgatcgatat aggagagaga ttctacctga ttcgtaccgc tgatttcttt cgtcgctgca
    17881 tgtttgataa cagtgacatt cgcaagagag actagtttat cctgtaagat ttgatctgct
    17941 tttaattctg gtaagaattc tagaacatat acatgttttg ctaacccagc caaatcgatt
    18001 gctgcttcaa caccagaatt accaccaccg ataacagcaa tctcttttcc tttaaataaa
    18061 gggccgtcac agtgtgggca ataagcaatt cctttatttt taaactcttt ttcgcctggt
    18121 acattgatag aacgccagtg cgcacctgtc gcgatgatgg cggtttttgc tttaagtgat
    18181 ataccgttag ctaagccaat attaataaaa tgttgctctt tatttaagga aacggcctgt
    18241 tgattcatca taatgtcgat agggtaacta cgcacatgac tttctacttg ctgcatcaat
    18301 tttggacctt ctgtgtaaga ggtaccaatc atattttcaa tccctagtgt ttcaacgact
    18361 tgtccgccga aagaatcagc gacaagtcct gtacggattc ctttacgtgc tgcatagata
    18421 gctgcactac tagctgctgg tccaccgcca attactaata catcataagg ttctttttca
    18481 aacaactctt cttcaatcaa agggccagtg attttttcaa caatttgttc gatcgtcatg
    18541 cgtccgctac taaattcttc tccatttaaa aacacagttg gcactgccat gattttttta
    18601 tcatcgattt ctttttggaa catcccacct tcaatcatcg tatgagtaat atttggattt
    18661 aatacagcta atatattcaa tgcctgtaca acgtcggggc aattatgaca agtcaagcta
    18721 acataactgt caaaatgcaa tgtttgtgtt atttttttga ttttaagact caaagattca
    18781 ctgattttcg gtgcccgtcc actaatttgt aacatagcca gtaaaaaaga ggaaaattca
    18841 tgaccgaggg ggataccagc aaaaacgatt ccactttctt tgccaactgg attgatagaa
    18901 aaactaggtg tgcgcatcga atctcttttt tcccaagaga ttttaggaga catttctgct
    18961 atttccttcg taaattcagc tagtttttga ctattttcac tattatccaa actaacagaa
    19021 aaaacaatag gagattcaat cagtgctaaa tattgttcga gattttgttt agtttctttg
    19081 tctaacatat ccaccaccta aatctttcca acaagatcaa aacttggttt caatgtttcg
    19141 ccactttctt tccattttgc cggacaaact tcacctgggt gggtacgtac atattgagca
    19201 gcgcgaatct tatcaatcag tgcactagcg tcacgaccga ttccatctgc attgatttcc
    19261 atagcttgaa cgattccgtc tggatcaata ataaatgttc ctcgctgagc taatccttgc
    19321 tcttcatcca aaacatcaaa aatacgagaa atctgatgag acggatcacc gatcatcacg
    19381 tattcgattt taccgatagc atctgatgta tcatgccatg ctttgtgagt aaagtgggta
    19441 tcggtagagc aagaaaagac ttcaacacct aaagatttca atgtactgta ttgttcttgt
    19501 aagtcttcta attcagtagg acaaacaaaa gtaaaatcag ctggatagaa gcaaagaata
    19561 ctccagttcc ccataagatc tttttcactg acttctaaaa attctccttg atgataagca
    19621 tgtgcattaa attcttcgat ttttttccca atcaatgaca tagtaaaacc tccaataata
    19681 attatttatt tataattatt gtaatttagt tggagttgca aagtcaatgg atattttgtt
    19741 atttaataaa gattactcaa aaacgaaggt taattataaa aatagaagag gtcgcaagaa
    19801 aatgctactg ctggaaatac tcgggataac gttgagtaac agtttcctgt tctttcagca
    19861 tcatgtgtaa gtgcaatccc gtttgaaatc gagcttcttc tggtttttta tcaactaaag
    19921 ccttgagtaa atcataatgt tgttccatga tcgtttccca tggaagcccc tgaatggtca
    19981 ctcgtaacca gcggaaacgt tctaaatggg tattatggtc atctaaccag ttccaaactt
    20041 cttttcttcc tgcaatttcg aaacaagctt catgaaataa attatctgca cggaaaaaag
    20101 cgcgttcgtc atttattttg actgcttttt cttgttcttc taaaatcgtt tctagcacta
    20161 ttcgtgtttg tgtgtccaat ttggaacaac attcaatcat gatttgctct tccaactgtt
    20221 ctcggacaaa gcgagcattt tctgcagaaa caagattaat tttggaaaca tacgtaccac
    20281 tttgaggtat cgtatacacg agttcttgct gacgtagttg aatcaatgct tcgcgaatgg
    20341 gagtccggcc gatagcaaga tgttcctcca gtgccttttc agatattttc cttccaggtt
    20401 ctaagtcagc atagataatt tgtttacgaa tcgcttgata agcttgtgat tgcaagctgc
    20461 ttttcatctt tattcctcca aaatcatttc tatgaacgta gtataacaga agaggtctga
    20521 aaaacaatct ggtatgtcac attaactagt aaatttgtat aaatagtaaa attttggttg
    20581 acatagcgat ttttattgta taaaattcac ttgaatacaa aaactaatat gttagtttag
    20641 gaggggaaaa atgattgatt taaacaactg gaggaacttt atccctgaat tatctcaaaa
    20701 agaagtagaa atacctcatt ttgaccgtgc atcattaaga gaaaatggac aaaaaaatcc
    20761 tgtctggatc catttcggtg gcggaaatct ttatcgagga tttcatgcag aaatcgctca
    20821 acgtttagcg gatctaggcg aattgtccac tggtgtcatt gtttgtgaaa cattcgatga
    20881 agaaataatt gaaagggctt accaagattt tgatgatttg attcttgaag tggtcatgcc
    20941 aagcagtgga gagttcgtca aacgtatatt agctgcgaca gctgacagtc tgtattgtca
    21001 tccagattgt tctgaacact ttgataagat gattcgacac tttgaaaatc ctagtttaca
    21061 attagttact tttgctatta ctgaaaaagg ttatcgattg aaagatacaa ataataaata
    21121 tataggaccg attgcagtcg atataaaaaa tggtccaaag aagccatcac atacaatgag
    21181 tattcttgct gcgctacttt ataaacgttt tttatctgga gcttggccga ttgcacttgt
    21241 ctctacagat aatttttcac aaaacggtag tcaattacgt acttctttgc taacgatcgc
    21301 aaaagaatgg caaaaggaag gttatgtaca aaatacattt atccaatact tatcgaatga
    21361 agaaattgtt gcgtttccat ggacgatgat tgatcgtatc accccgaatc cttccttgtc
    21421 agttagcaaa atgttggcaa aagagggctt ttcaaatatg tcgattttcc aaaccgcaaa
    21481 aggaaataat attgctccat ttgtcaatac agaggacatc cattatttaa tcatggaaga
    21541 tcattttccc aatggacgtc cgaaattcga aaaagctggg gtattcttga ctgatcgtgc
    21601 aacggtagat aaagcagata cgatgaaggt gacgacttgc cttaatcctt tgcatacgtc
    21661 tttagctatt atgggttgtt tactggggta taggagcata gctgaggaga tgaaagatcc
    21721 agatttatcg ctgctagttt ctgaaatcgg gtacaatgaa ggcctcccag ttgtggagtc
    21781 tccaggtatc attgatccaa agatctttct ggaagaagta attactgtgc gcttttctaa
    21841 tccaaatatt cctgatatgc ctcagcgaat cgccacggat acctctcaaa aggtggcgat
    21901 tcgttatggt gagacgatta aaaaatatat ggaaatagaa gttaaagacc cgcagacact
    21961 tgtattcata ccattgactc tcgctgcttg gcttcgatac cttattggca ttgatgatca
    22021 aggaaaacca ttcatcccga gtccagaccc actattgaca gactttcagc ataagctttc
    22081 cgatattcaa ctgggtacgc aagatacatc acatattcat gagaaaatgg ctcctatttt
    22141 gtcaaataaa gaaatttttg gtgtagatct ttaccaagca gagcttggca gaaaaataga
    22201 agaaatgttc atcaagatgg tagcaggacc gaatgccgta cgaaaaacat tacaggatta
    22261 cttacaagaa catggaggaa atttatcatg gaaatgactt ttagatggta cggatcacag
    22321 gaagaaaaga ttaccttaga tgaaatcaga cagattcctg cggtcaaagg gattgtaggc
    22381 acattgatgg atataccagt aggagaggta tggcccaggg agaggatcaa agaactaaaa
    22441 gaagaaattg agtctcgtgg gttaacacta aaagtaattg aaagcgtcaa tgtgcatgat
    22501 gccattaaaa ttggattacc ttcccgtgag cagtatatcg aaaactataa gcagacaatc
    22561 cgaaacttgg cagaatatgg aatagaagtc atctgctata attttatgcc tgtatttgac
    22621 tgggtgaaat ctgaccttga ctaccgctta gaagacggct catccacgtt agcttttata
    22681 tctgatgata ttccagatga tccaagagaa atcgtcgcgc gcatcgaaca ttccagcaat
    22741 gaatttgaat tgccgggatg ggaacctgag cgcttagctc atatcaaatc cttgcttgaa
    22801 gcctatgcat cagtagacga agaaaaactt cgagaaaatt ttgcctattt tctccaatca
    22861 atcatcccaa catgtgaaga ggtaggggtc aaaatggctg tacatccaga tgatccgcca
    22921 tactcgattt ttggattacc tcgaatcgta aagaatcgag aagatttaga ttggttgtgc
    22981 cgcatagtag acagccctta taacggaatc actctttgta cagggagtat tgcagaagat
    23041 ccagaaaatg atgtatatgg gattttagca gaattttgtc aaagagatcg aatcccattt
    23101 gcacatgttc gaaatattaa atttttagct aatcgagatt tctatgaggc accgcataaa
    23161 tccgaatacg gttcattaga catgtaccgc atactaaaaa caatgtatga caacggattt
    23221 aacgaataca tccgtcctga tcatggaaga atgatctgga atgagacagg aagaccagga
    23281 tacggcttat atgatcgagc attaggtgct gtttatctaa atggattatg ggaagcaatc
    23341 gaaaaaggaa acaaaagtaa agataattag tgaaaaaaac tgtaatttta ttatgtaaat
    23401 aagaaggatg ttcaaattct tgatacataa atccggaggt tgttaataag cattcggatt
    23461 tatgtatctt atttggtcta ggaagtaact tgattttctt ttttcgattg atccatgagt
    23521 gcttcttctt ttaattcaaa gatatattct gagatcctca taatcacttt tttccagtct
    23581 tctggtgtga tgattccttt taaaatatag atttttttga tatgagaagg caattgtttc
    23641 aaagggcagt cgctgataac gatgttgcag ttctccataa aatcattttg tattttgatc
    23701 gattcatttg aaaaaatttg ttctatctta acgatcagat ctttactgat ataaaaatca
    23761 cgcgtatagt tcacaccaat cacaattcgc ggctgttttt tgtagcgatc atacaaatgt
    23821 actaggtctt caaccatcca ttgcacattt tccttttcta aaataactgg taacaagtgt
    23881 tgattttgtt ctctgaaatc catataaaat tgttcaatgg cggtttggac ttctttcaac
    23941 acgctatgtt cttgataaag catgaaagta ggaaggggcg tatttggttg attgaagtta
    24001 agaaatccat tataaagttt atttcgtgcg aatgccaatg agtaaagaat cttttctttt
    24061 tcaggtataa acaaatcaaa tacagtacaa aaggactcca ccatttctct tgaataaaga
    24121 acatgaggaa cgtttgcttt ttttaaaagt ttgtattgct ccaagcttgt ttcttccgat
    24181 tcgattttcg tgatagaaag tcgagctaaa atattcacta agatgcgttg ttcgcgacaa
    24241 attggcagat taggtcgcaa aatatctaca gagggatcaa taaaaaagtc aatatattca
    24301 tactctgtga attctttttg tacgtctttc acagaagttg caggaaaatt ctctttacat
    24361 atttttaata gaaaatataa tttatctatc ataccgctat ttagtcggtc aagtacttct
    24421 tttttaaaaa aagtattcat cataaaagcc actacttgcg gatcttcatc ataaaattct
    24481 gtagcagtac ttgaaaagat gttccaataa atatctaatg ctgcatattg aatttgtacc
    24541 tcttttccag aaaaagactt tctgccatta ttagaaaagg agatagacaa accgtacaat
    24601 gctaaaaatg cattaatctt tttcattttc gtatacaaat aagaggcact atagtttgtt
    24661 tgctgactta aactgataat cgaatttgtc tgacagctga acaaagaagt caagatctga
    24721 taactgactg attcgttgca gtatcttcca aatagacgat aatagcagta agccggatca
    24781 attccttcaa aattcaactt taaaaaaggt ttttgcttat caatatgcag ttctgttaga
    24841 ttgagacgct caaagtcttg ttctaattct ttgatgtata gtaaaaaatt tgattcagac
    24901 atatggaaat gatctgctaa atattgtgta gtaataaaga aacgatgttc agataggaaa
    24961 agaaaaatat ctaattttgt attgcttttt ttcttcaaga gaaacgtttt aatcgcttct
    25021 tgagtttgca tgaaagaggc tcctttttac ttaaattgtt aaaacttgaa aatattactg
    25081 tttttttcat aaatttagac aaaaaaacag taaacatata ggtagaatct ctactatctt
    25141 atttcttgca tacaaaaaaa tcaaggagtg agagagttat gagatattca accatcttaa
    25201 tatcaacatt tgcagcctta agcttatcgc ttactataaa tcagacagtc cacacagagg
    25261 agatagaaac agccaaatgg acagaaacca agtgggccaa cgtcaatcga attacctttc
    25321 aggatgaatt ttcatccttc aattcaggag tcgcaaaatt aagaaaagga caaacagcga
    25381 ccatcaaagc aaaaattgac tattcaggag acttagcacc tatactacaa tcagctgtta
    25441 catttgagaa tgttgatccc gctttgtcga ttggctatga cgagaacact ttgactatcc
    25501 aacacaagca agcgatattt accttcaccg ttacattaaa tcaggacttg ttaaaaccag
    25561 cgctttttac tgtcaaggta tcagatggac taccgaacga tcatcatcat ttaacgccgt
    25621 atagtcagcg gcagacaatt gagcaagatt cagctatcga ctcaggagga gatcttgttg
    25681 aagaaccaac agataaacca gaaaatgaaa ataagccaga agtaccgcct acagaaaatc
    25741 cagacggaga acagaaaccg gaaatagaac ctggagaaga acccgataca gaaactcaac
    25801 cagagccaga caatgaatca aaacctgaaa taacccctgg agaaaaacca gatgtagacc
    25861 cagaagaaaa accagatgtg actccagaac cagatacgga cgcaagagac caaggaatac
    25921 cagaaaaaat aaacaaaaaa acaattcaag aggatggaaa aaaagagagt aaaaaatcaa
    25981 atctagcaat tctaaaaata aatgaagagc agctgaataa aaaatcacga atatttgata
    26041 gcgcccaatc agctgaaacc ttaaaaagta gcaaggatac aacctttgct gtcctgaaac
    26101 gaaaaataaa cagctaccaa aatccggaga gtcacaaaac aaagttatac tgtggagcgg
    26161 aatcattcta ttaagtatag caacaatgct ttctgcaaaa agatttaaac aaaacagatc
    26221 tctctaacat ttgaataatg tctgtgaacg catttttcaa ataaaaagaa atatccagaa
    26281 aatgacccca aaataccaga ttgaaatctg atatttgggg tcgttcatac tttatctttc
    26341 ttcatattta ttttaaggga tatctgtttc ttttggaaga tggttcggga tctttttcac
    26401 tttcaataca tctaaaaaga aggtaaagat cggaatgccg acaatcaagc cccaaacgcc
    26461 aaagaaccgt tcgctaacaa gtaagaccac aaatgtataa aaaatcggca gttctgtttt
    26521 gctggacatc aacttcggat tcagcacata agactcaatt agatggacaa tcaaaatcgt
    26581 aatcaggata taaacaacat ctcggatgcc tccttgggaa taagctatga aagatagagg
    26641 aatacaagaa atgataacac ctgctactgg gatcagactt aaaatgaaaa tcatgatcgc
    26701 taaactcagc agctgatgaa agccaaatac agccaaggcg attgtagtga gcaacgtatt
    26761 taaaagagcg acaacaaatt gagcttctaa taccaaacca aatgtattga caaacttatc
    26821 ggcaaaatag taaatatctt gaaagaacca agaaaattca ctttttaaaa acaatcttga
    26881 gaatacaatt gtttttttct tttcaatcat aaaaaagaaa cttaagatga atgacatagc
    26941 aaacgccact gccatttttc ctatatcctg taaatagcca agcgcaatcg aagcgccatt
    27001 ttgaagctgt tcaaaaaaat cgtttcgctc aatataagta tggatatatt gtaaaacagg
    27061 atctgcatct tttggctgat gctcgtaaaa attaataacg gactggatca tgtcaaatgt
    27121 ttgatgaata agcatcggga tatactttgt gaatgccaga tagataaggt aaacgatcag
    27181 tgtataaatc atgatagcta aaacacctgt agggattcgg acaaatcgct gaatgatttg
    27241 aacaagatga atcatcaaat aagtaaagat aaatgtgagc aaaatggttg taatcatact
    27301 tctaactaag aaaagaacaa aaacgacaaa taataaaaca gtgagacggc gtattttttc
    27361 attcgccaaa aaacgctgat aaacggtcaa atcgacaacc tccaaataac aaaaaataaa
    27421 cacgttattc attatatgat ggaatatgaa aataagaaag agcaaaaaga aaaaccgcta
    27481 aatgacgtta tcttatgagt tggttttcct cataaaatcg ttcatctagc agtttcagcc
    27541 ctttttctgg tcttgatgaa aattatctag catttttcgg gtcagatgtt gcagtgtatc
    27601 caattcttcc tgttcaagtg gaagataaga cttgatcttt tgtggaatac cttctgcttg
    27661 tttttgcaac gcagcacctt gagcagtcag cgtaacattt acgattcttt catcacccgt
    27721 ttcacgctga cgattaatat aatttttttc tgctaacttt tttagtaagg gagtcagcgt
    27781 accagaatcc agacacaaat catttcctag ctctttcact gaagtctttt tcttttccca
    27841 taaaactaac atcacaagat actgggtata ggtaagattc agcggcttga ggaggggagt
    27901 gtagtgtcta atgatttctt tagaggcggc atataaagga aaacatactt gattaactaa
    27961 atatagatct tccatttttc cacctccgct gttttactat agtgtatcat agcggattta
    28021 cacaaaaaag tctattcact aactgctttc agagtaactt ctatatttcc agctgtagct
    28081 tttgaatacg gacacacttt atgcgctgcc tctaataatt cttgcgtttc ttccagagaa
    28141 agaccttcga tagatccttc aatctctaca cctaagacaa agctcgtttc actttctgaa
    28201 taaagggaaa cttgtgcact gacagtagaa gccccagaga tatttttttg ctgtttgatc
    28261 aattctaatg cactgttaaa acatgcactg aaaccagctg caaacaattg ttcagggttg
    28321 gttgcatttt cgacgcgttt accaggagct gcaatattca attcgaatgt acgatcggga
    28381 ttatgcactt ctccagagcg tccgcctgtg ttgatcattt tcgtagaata catttttttc
    28441 ataaaattac cacctttaat tatattttat acaattgaat tttacacaat ataattgtgg
    28501 aaagcaaaga aatggattgg cataagataa ataagagaaa gcacatgaaa cagacgcgct
    28561 ttctcgaata gcaaacatat acaaaaaata tcagagacaa aaaataatct catcataaac
    28621 attacatata tttctcaaaa agttgttttc tttcttcaaa aattacttca cgatggacct
    28681 gatgaagcat ccataaataa ccaaaaggat ctctgaacat tgagttagtc acaccaaagt
    28741 ccatcatttc tgtcatttct tgaatctcta cacagccatt tcgtaatgct ttattatgtg
    28801 tgtcctcgat atcagagacg atgatattga accagatcgt atttggatgt tcagcagtag
    28861 gagcagtcaa accaagagac gtgttttcat ccagcatatg gaatctcgta ccatacaagg
    28921 taaaaatgac ttcattattg cctctagggt aatctgttgc ctcaatcagt tgtatttcat
    28981 caaagattgt ttgatacagt tctagtgctt tcaaactatc atttaataca aaatcgattt
    29041 ccgttccttt atgcataaag atctcctctc cttttcagtc gaacagttac tagattcttt
    29101 tattatacca gctaaagaaa acgcattcaa aaaaatagcg gatttactta ccaactcatt
    29161 cgattggatg ataagtaaat ccgcgtccca ttacttctgc tacgctgctg acaatgacaa
    29221 aggcacttgg atcgatttct tctatcaatg gtttcattcg ggaaaattca tgatcagtca
    29281 caacgatcaa taaaacctct tttgactttc gattataccc gccttttgcc tctagcaggg
    29341 taaggccacg tccaatctca gcacttaagc gaaggcggat gtcttccaaa taatgttcac
    29401 tcatgaccat aacagatttc tttcgattga tacctgtttc gatataagtc atgacaattg
    29461 aagtgattgc aatagataaa atagcataaa gaaactcttc tattccaaaa ataaacagat
    29521 taagagaaac aacgaatgcg tctgtaaaaa ataagccaat cgaagtattc aagtggaaat
    29581 attttttaaa aatgagggga gggatcgtcg taccgccgct agaagagttg tttttatata
    29641 aaatcgcaac tcctaaagca aaaatcgcac ttccaaaaat gacagacaac aaccgatctg
    29701 aagtaatcat tgtttctgga actattgcaa tgattaaagg aaaaaccaag cttccgaaaa
    29761 gtactttaaa aaagggtttt ttacctaaaa atagaagagc tagtaccaac ataacgatat
    29821 tcaacactaa aataactata gctcggtcaa agcctaaagc agcttccagt aatattccta
    29881 gaccgctgac acctccagca gcaatatgat gaggccctaa aaacatattg atagcgactc
    29941 ctaatatgaa gagggcaaaa ataatcttaa tgtatgtcca tataaattgt ctcgtttctt
    30001 tcatttattc acttccttaa ttcattaata atactcttat cttattataa cttacttttc
    30061 accttaagtt aactttaatt aataaaaaaa ttaggctagt ctaaattatt atgtttcatt
    30121 cttctagaat atacttctac gccaaaaact gttaaaaaac ttttacttta gaaaattgga
    30181 gaaattgatc gcttaagata aattcaggac actaaatagt acaggaagta gactaaaact
    30241 tttaaaagtt ttaagtctat ctatttggca agtttaaaaa ataaaaattc ggtatcaaaa
    30301 aactgttacc gataacattt ttgaaagcgt tgtcttttgc ttctctatca cttatagtga
    30361 tgacgtcata ttaaaacaca aggaggaaat caatggatac agctgcaatt tatcatcgac
    30421 cggaaagtga atatgcattt ctatatacga atacaagatt tcgtattcgg cttcgtacta
    30481 ggaaaaatga tatcaaaaaa gtatatgtgc tatgtggaga tccatatacc ataacaactg
    30541 aaaaatggta tcaaaaacaa cgaccaatga aaaaatgtct aagtaccaat gttcatgatt
    30601 attgggaaat tgaagtgacg agcgaaacaa gtcgcttaca gtatgcgttc catgttgttg
    30661 gggaagataa cacggactgt ttttacggtg atcaaggaat ttttccttat cgagaaaatg
    30721 tgattactga accaaatttt tattttcgaa taccttattt tcatcaaatc gatcgattca
    30781 ctattccaga atgggtaaaa gaaacagtat ggtatcaaat ttttcccgaa agatttgcaa
    30841 acggggacaa agcaaatgac cccgaacaca ctttaccttg gggaagtaaa gatccagacc
    30901 gagaagactt tttcggggga gatttgcaag gtgtgatgga tcatttggat tacttgacgg
    30961 accttggcat caatggcatc tatttttgtc ccatatttaa agccacatcc aatcataaat
    31021 acgatacgat tgattattat gaggtggatc ccgcttttgg cgataaaaaa cttttaaaaa
    31081 acttgattga tgaagcacat aaaagaggga tacgtatcat gttcgatgct gtattcaatc
    31141 atatgggtgt ccattcacca cagtggcaag acgttctcaa aaacggagaa aaatcgatct
    31201 ataaagactg gttccacatc cgttttttcc cagtagattc ttatcagatg actgacttac
    31261 cagaaactgc tgaaaatatc ccatatgata cttttgcttt tactccattc atgccgaaac
    31321 taaatacagc aaatccagaa gtgcaaaaat atttgctgga tatcgcaact tattggataa
    31381 aagaattcga tattgatgga tggcgtcttg atgttgctaa tgaagtcgat catcattttt
    31441 ggaaaaaatt cagagaagca gttacagaaa tcaaaccaga catctatatc cttggagaga
    31501 tttggcattc ctctcaagct tggctgcaag gggatgaatt ccatgcagtg atgaattatg
    31561 catttacaga ttcgattaaa gattattttg caaaaaagaa aatcacagct tcacaaatgg
    31621 tcagtggaat gaatcatcag caaatgcttt acagagatca ggtgaacgaa ggaacattca
    31681 acttattaga ttctcatgat actgccagga tcttgaccct ttgccaaggt aacaaagaat
    31741 tgatgaagtc tgtgatggcc tttatgtttc ttcaaaaagg gtctccttgt atctattacg
    31801 ggacagaaat agggatgact ggtgaagatg acccggattg ccgcaaatgt atgatatggg
    31861 agaaggagga acaagatcta gagctattcg gttttataaa agaacttgtt tctctaagga
    31921 aacagttgtc taagattatt tctgaaggtt ctactcagtg gctaatagta aatgatcgtg
    31981 aagataagct ctatttcaca cggaaactag agggacagat catctatgtt tactttaatc
    32041 agtcaaaaga accttgggta gtagaacagg aaaatgaggt gattcttagt caaaattgtc
    32101 agttgttaga agacgggaaa gcagagatac gacaatacgg atttttgatc atgtgttgaa
    32161 aactgttacc ggtaacaaca agaaagcgtt tgcaaatgaa gatttgtgcc cattataata
    32221 agggtgtaac agcacgttga atacaaagga ggaaatgaag atgaaaaaaa gaagtgtgaa
    32281 acagttgggt ttagggttaa tctctttagg aatcttaggc ggattagcgg cttgtggcaa
    32341 tggtaactct tcagcagaag gggaagccga tgaaggaaaa acgttaacag tttccgttga
    32401 ttctggatat aaagattata taaacgaaat aaaaaacact ttcgaaaaag aaaacgatgt
    32461 gaaaatcaag ctagtagaaa aggacatgtt cgatcagttg gaatcattag cgttagatgg
    32521 gcctgctgga aagggacctg acgttatgat ggcagcatat gaccggatcg gtgcgttagg
    32581 acagcaagga catctggcgg aagtaaaact gggaaatgaa gcagcttacg atgaaacaga
    32641 caaagcccaa gtgacttatg atggaaaaat ctacggagaa cctgcagtaa tcgagaccct
    32701 tgttttgtac tataacaaag atttagtcga tacagcacca tcaacgttta aagatttgga
    32761 gaatctatcc aaagacagtc gttttgcttt tgagtcagaa gcaggaaaaa atacaggatt
    32821 tttagctaaa tggactgact tttattattc ctatggttta attgcaggtt acggtggcta
    32881 tgtatttggt gatgatggca caaatccttc agatatcggt ttgaataatg caggtgctgt
    32941 agaaggaatc tcttatgcaa cagattggtt caaaaatgtt tggccaaagg gaatgcaaga
    33001 cataaaaagt gcaggagact tcgccagcca gcaattcatg tccaataaaa ctgcagcaat
    33061 cattgacggt ccatggcaag cgcaaacata caaagaaaac aatatcaatt atggtgtagc
    33121 caaaattcct actctaaata atggacagcc ttatcaaccg tttggcggag gaaaaggatg
    33181 ggtagtcagc aattatgcta aaaacaaaga cctctctcaa aaatggcttg attatgtaac
    33241 gaatcaggaa aatcaagaaa aattctttga aatggtaaat gaaatccctg caaaccaaca
    33301 agctagggaa acagcaaagg ctaaaaacga tgaattaaca acagctgtga ttgaacaata
    33361 tgaaacagct caagcgatgc caaacatccc agaaatgggc gaagtttgga caggtgcaga
    33421 aaacttgatg ttcgatgcag catcaggttc aaaaacacca aaagaatccg cagatgaagc
    33481 agtaaaaaca atcagtgaag ccatagaaca aaaatactga ttaaaatgat aaccgtccat
    33541 ttttataaaa aatggaacag gtgcttcaga taaaagtggc aagagcgaga tggaaaatta
    33601 tcttgctctt gttgccatta tcttaaaagc agtttgcggt ttagcaagtt tagagggggc
    33661 attcagatgc agcaagataa aacaagcaat atcaacgaaa aaaaaatcac gctttgttca
    33721 cttattccag ggatggggca gtttgtaaat ggccaagctt ttaaaggtat cgtttttttc
    33781 gccatattcg tcctattcat tggtcagatg cttctgggcg gatttcatgc attggtagga
    33841 ttggttactt tgggaagtat accaatggaa gatcattcat tatttatttt gattgaaggc
    33901 actttgcaat tcatcattac aatcattttc ttgatttttt acggtttgaa tcttttagac
    33961 gcttatcgag tagcgaagat gcgtcaagag ggcaaaaaaa tcaatcggac gatgaaggaa
    34021 gtcatacata atataggtga tgaaggtttt ccttatctat tgacacttcc cgcttatctt
    34081 ctcatgattt ttacgatcat tttcccagtc ctagttacat tgttcactgc atttactaat
    34141 tatgacttca atcatattcc accagccagt ttaattgatt gggtaggggc taaaaacttt
    34201 tttagtatct tctttttaag ctcatatcgt aatacttttt ttgcagtctt ttcgtggacc
    34261 gtgatttgga ctttatgtgc aacaactttg caaatcaccc ttggtattct cacagcggtt
    34321 gttgctcacc agaaatttat taaattcaaa cgtattttcg gtatcatttt cttattacca
    34381 tgggcggtcc cagcttttat tactatcatg agtttttcga atatattcaa cgatagcgta
    34441 ggtgccatca atactcaagt gattcctttc ataaaccagc ttccttttgt agatatcagt
    34501 gcaatctctt ggaaaacgga cccgaactgg acgaaagtag caattatctt agtgcagggc
    34561 tggttaggat ttccctatat ctatgtgatg gttacaggaa tcttacaatc gatttcagaa
    34621 gacttatatg aagcagcaaa aatcgacgga gcaaatgcat ggcagcgctt ttcaaacatc
    34681 acgcttcctg ctgtcttcct agtggcagcg ccaacattta ttacacagta tacggggaat
    34741 ttcaataatt tctccatgat ctatctgttt aacaatggcg gtcccggaag cgtaggcgga
    34801 ggagctggtt ctactgatat tcttatttcc tggatctata aacttacgac tggaacttct
    34861 ccgcagtatt ctatggcttc agcaatcacg ttgattatct ccgtcatcgt tatctgtgtg
    34921 tcattgctgg tatttaaaaa gacaaatgcg ttcaatatgg gggattaaca atgaaaaaat
    34981 ttcattcgag tgctcgattc caaaaacgac tttctcactt ttttacttac ttttttcttt
    35041 taatactgtc agtcattatc atctatccgc ttttgatcac agcaagtact gccttcaagt
    35101 caggaaatat tgcagctttt agtttagatc tttcaagtga ctggacttta ggaaacttta
    35161 ctcggctatt cagtgaaaca ctttacggga cttggtataa aaatacattg atcatttctg
    35221 tcctaacaat gatcgtacaa gtgactgtag taactttggc gggctatact tatagtcgct
    35281 atcgttttat cggccgcaaa tcaagtttga tgttcttttt agtgattcag atggtaccga
    35341 ctatggcagc attgactgcc ttttatgtca tggctttctt attagaagcg ctcgataagt
    35401 actggttctt gacaatgatc tatataggcg gtggaattcc aatgaatact tggctgatga
    35461 aaggatactt tgacactgta ccaatagatt tagatgaatc tgctaaactt gatggggcag
    35521 ggcatttcag gatttttttc cagattgtat taccgcttgt caaaccgatg ctcgccgttc
    35581 aagcattatg ggcttttatg ggaccttttg gggatttcat gttagctaag ttcctgcttc
    35641 gaacacctga aaatcagact gttgctgtcg gcttacaaac atttatttct gatgtgacaa
    35701 accaaagagt ctctcttttt gctgctgggg caattttgat tgcactaccg atttgtctat
    35761 tattcttctt tttacaaaag aactttgtta ccggcctgac atctggcgga acaaaaggat
    35821 aggagcagag tatcatgcaa acatttccgt taaactattt ttccgcatta cgtacgccaa
    35881 ctcaagtgtt tgcgggacgc aaactattat catggcctaa atttttcttg atatttgtat
    35941 ttttagtttc cttgatggtc atgcctgtga ctctttttta tgccaatcag atccaagcga
    36001 ttccattaga acaatttcta tccgttcatt cgctgatcga tgaacaagga acccaaaaat
    36061 tttctgaact tgaattatct gaaactgggt tacaagcatc gcaacaaacg atagctgtga
    36121 cccctgaaat tttggtaggt gtgtctttat ctgagaaaca acaatccgat catggaactt
    36181 ttattgattt tgagaaggaa caatgggtga tccaacaaaa ggataaatct ggtatacgcc
    36241 gctatacgat gaattatagc ccatctttcc aaccagattc tgtacgtacg cccgaagatt
    36301 tccaacgatt tttagaaaga gaattttatg ccagtaatcg tccaacaatc attttatctt
    36361 atagtttaag tcttggtctt gtcctatttg tcatgacgag tctcatttta ttcggggctt
    36421 cctttttctt gtggatgaca agaaaaagcc aactttcatc cattcataca ttcaaagaga
    36481 gtgcaaattt gatgctgaat gttatgggaa taggttccat ggcagcagca gttgtagggt
    36541 ttatccactt tgactttatc ttgatgctaa gtgtccaaac aacgataact gtcttgctgc
    36601 ttttatgggt ttttgtcaaa acaagattca aagataagcg tgtcgaatga caagtttaag
    36661 cgatttatgc gacaatgaat gtgaaaaaaa tgaaaaataa agtagttttt gaaaaatttc
    36721 tgaaaaaatt aaacttctta gttgattctt ttctttttta gtattaacgt aatagggtag
    36781 tttttttaag gaggaaaatc atgttagaga aaactttata caatcaatta ttttcacgtt
    36841 ctttctcgct ccctgtcgaa gtaaattatt gggacggtac aacgaaacaa tacggagata
    36901 cggacactcc tccccaaatc aaaatcaata tccatgaaga aatcccaatg aaagagatta
    36961 caagtaacgc ttctttggca ctaggagaag cctatatgga tcatcgaatc gagatcgagg
    37021 gaagtatcca agcattgatc aacgatgctt atcagcaagc aaacagcttt ttacgaggaa
    37081 aagattatct aaaatggctg ccaaagaaag aaaaacatac gaaacaaaaa aacaaagaag
    37141 atatccatgc gcattatgac ttaggtaacg acttttataa actttggcta gatccaactt
    37201 tgacttattc ttgtgcttat ttcaatacac ctgaagatac gttggaacaa gcacaaatca
    37261 ataaagtcca tcatatctta gacaaactct ttatcaaaga aggcgaaacg ttgcttgata
    37321 tcggttgtgg ctggggaaca ttgatgttca ctgctaccaa agagtataat gtcaaggcaa
    37381 ctgggatcac actaagtgaa gaacaatacg atttgattac caaaagaata aaagaagaac
    37441 atttagaaga taaatgccgt gtattattaa tggattaccg cgaactaaaa ggcgaaacgt
    37501 ttgatcatat cacaagtgta ggtatgttcg aacatgtagg ttctgaaaat cttgaaggtt
    37561 atttcaaagt ggtaaaagat ttattgaaac caaaaggaac agctttgatc catggaatca
    37621 gccgacaaca aggcggtgcg acaaatgctt ggatcaataa atatattttc cctggcggtt
    37681 atgtccctgg tgttactgaa ttagttgata atattacaaa aaacgatttg caactgattg
    37741 atttggaaag cttacgtcgt gactaccaat tgacattaga acattggaca aagaattttc
    37801 atcaagtcca agatcaagtc acgaaagaaa tgggtgattc cttctatcga atgtgggatc
    37861 tatatttaca agcttgtgca gcttcatttg aatcaagtaa tatcgatgtg attcaatatt
    37921 tgcttgttca acctggaaac aatgacatcc caatgcatcg tgcataattg aggcaaaagt
    37981 actgtttaaa gactatttaa aaagaagcta agttttatga tgctccagaa gtgaactttt
    38041 ttgtctaact tctaggattg tctctaaagc ttagtttctt tttctttata ttttcataat
    38101 aaaagtttgg ttctttaatt tttaaacatg acagaaaatt ttttcttcgt tatacttgta
    38161 atagaatact attttataac aaaggggatc gaatcaatga aaaaaactgt tatttatact
    38221 agtttatttg cagctttgtt tttagctgct tgtcaatcaa ataccaatca aggcacatct
    38281 gggaatacgt cttccagtag cgaacagcgc tcttcacagg tatcggactc ctcaacaaaa
    38341 gcaagcacat cttcgtctag ccagacaaaa agttctagtg aagcaagtac tcagtcagat
    38401 atcaaagtgt ctgcagctcg tgcgatcgaa ttgttccaag ataaataccc agatactgca
    38461 attactagtt tggaattaga tagtgattgg ggatcttact tctacaagat agaaggtgtc
    38521 gatgatcaaa aagaatacga agcaagaatt gatgctcaaa atgaaaaaat ggagacagag
    38581 aatccagaaa ctctagatcg agatgaacaa aatggtcaaa agaaaaaaga agatggtctg
    38641 gatgttgata aattgatcac gattgaggaa gcagcaaaaa tcgccgtcga caaagtagga
    38701 agcggtacgg caacagattg ggatttggat aaagatttag gtactactta ttgggaagtc
    38761 aaagtaaaag acggcaatca aagtacatca gtcaagatca atagccaaac aggcgaagta
    38821 ctgtcaactg aacaagatga ttaagaattt cttcggaaaa caagagttga tttttctttt
    38881 agtcatgata cactagacaa aataacttgt ttgaaggagc gatttacttg agtttagaaa
    38941 cgacctttgc caatcatatc tttgccaatc ctttgatgaa tgcatctggt gtccactgca
    39001 tgactaccca ggaattagat gaactggctc attcagaagc aggagcattt atcactaaga
    39061 gctgtacgat caatgaacga aaaggaaatc ccgaaccacg ctattttgat gttcctttag
    39121 ggagtatcaa ttctatggga cttccaaact taggtttttc ttattatcta gagtatgctc
    39181 ttgcgtatga aaaagtgcag gaaaaccaaa atcaaccgtt atttttttcc attgccggga
    39241 tgagtgtcca ggaaaacttg gaaatgcttg agaaaataga aaaaagtggg tttaatggga
    39301 tcactgaatt aaatttatct tgtcccaatg tgcctggaaa accacaacta gcttacgatt
    39361 ttgaggccac ttatgaaaca ctgaaagaag tattcagcat cttttctaag ccgctaggaa
    39421 tcaaattgcc tccttatttt gattttgctc attttgatca gatggctgat atcttgaatc
    39481 agtttccatt gacctatgtc aatgctatca acagtgtggg caatggtttg tatatcgata
    39541 cagaacaaga agcagtagtc atcaaaccaa aagaagggtt cggcggcatt ggcggagagt
    39601 atatcaagcc gactgctttg gcgaatgttc gcgcttttta tacacgtttg aaaccagaaa
    39661 tccaaatcat tggtacaggt gggattcgga caggacaaga tgcatttgaa catctgctat
    39721 gtggcgcaag catgttacag attggtaccg agcttcataa agaagggccg gaaatttttt
    39781 ctagaatcat aaaagaatta acgcagatta tgtcagaaaa aggatatacg tcaattgacg
    39841 aatttaaagg caaattacgc accatttcat agcaaagaga cgaaggtcat gattgatctt
    39901 cgtctttttt tataaaaaac actcgtctgt tcattcctgt gcaagcgtac tcattgtatg
    39961 ttagacgaac ttgtgggaaa ataacagagg caaaaaggag gataatatga atgatgcaac
    40021 agaattagca aaagagctta aagaaaaaga atttagtttt caagaactag ttacggatat
    40081 acagaaaaaa ataaagaagc aaaatcatga attgaatgcg tttgtcgcgc ttgaaccatt
    40141 cgagagtaat tcccacgagc tattgattcc ccagtcagaa aaagggccat tggctggtat
    40201 cccatttcct ttaaaaatgc tcggtcaaga aaaaaaggga tggctagcta cttcaggatc
    40261 tcgtttattt gaaacacacc gagcttctca tacatctaat tatgtgcggc aagcagaagc
    40321 gataggtcta gttccttttg gacaaaccaa tgcgccagag tttggtttca aaaatattac
    40381 agacccagtc atctatggcc cagcaagaaa cccttggaac cttgaatgta caccgggcgg
    40441 atcaagtgga ggagctgctg ctgcagtttc atcaggaatc gttcctttag ctggtgctag
    40501 tgatggcggt ggttctatta gaatacccgc ttcgttttgc ggattgattg ggctgaaacc
    40561 ttcgcgaggg acgatgccag ttggtccata tgcttggaga ggatggcaag gagctgctat
    40621 tgatttcggc ttgacagttt ctatgagaga cacggaggct ttattttatg ggatgagaag
    40681 tatccattcc ggtgcgccat atcaagtatc gccagttgag tggcaaacac atccaagaaa
    40741 aaaacggttg aagatagctt tttgtgttga ttcaccgatc cactcacaga ttagtgaaga
    40801 ggcagtactt gctgtaaaaa aagctgtaga tttccttgga aaagaagggc atgatttgac
    40861 agagattcct tacccactag atggtcgtaa actgatcacg tcttattatc agatgaatgg
    40921 cgcagaaaca gcagcaatga tccgctcgat tgaaacaggc atccagcgag ctgtcagaaa
    40981 agaagaactt gaacccattt cttggacttt gttccaatat ggtcagaagc tctctgccgc
    41041 tacttacatc gattctctgc atgtatggga tgaagcagca attgtcatgg aaaacctaca
    41101 tcaagaatac gatctattcc tttcaccaac gactgcattt accgccccta aaatcagcga
    41161 agatttgcaa agtgatgcga taagagcaca gatggaacac atcagtgatt gttctgaagt
    41221 agaaggttta gacgttgttt cttccatgtt tgaggagagc ttgaagatca caccttatac
    41281 acagttggct aatctgactg gacaaccagc aatcagcttg cctactcatt tgacaaaaga
    41341 aaacatgcct ctaggaatcc aatttatggc tgccagagga agagaagatt tgctatttca
    41401 aatcggctat ctatttgaag caaatcatca gttccattta ccaaatccat ggaaatagca
    41461 gaaacaagga gaagaagaat gaagaaaaaa agattgtttg ctctaataac tagtgccttt
    41521 tttattttaa gcgcttgcgg tacagctgcc gctaatcagg aagataacag ctggagtaat
    41581 gtagaaaaaa agaaagaatt agtagtagcg acttctggca cactgtttcc cgcatcttat
    41641 tataatgatc aaaatgaatt agttggttat gatgtagatt tggcaaaaga ggtggctaaa
    41701 cggttaaatg tcaaaatctc ctttaaagaa tacaacgtgg atggacaaat cacttctgtt
    41761 tcaaaaagag aatcagattt tgctgcgaac gattttggta tcacaaaaga aagagaaaaa
    41821 aaattctccc tttcagagac tttcaaatat tcatttgaca gtatgatcgt acgaaaaaaa
    41881 gatgattcag gaatccactc acttgaagat cttaaaggga aaaaagcagc tggcgaaccc
    41941 aatactagct atatgcgttt agcggaaaaa cacggagcta aattagtcac atatgacaat
    42001 gccacgaatg atcagtattt aagcgatgta gcaaatggtc gaacagatgt tattctaaat
    42061 gattattatc tgcagaaaat ggccgtatct gctttaccag atatccctgt aaaaatactg
    42121 gagaacgttt atttcaatga aaacgaaagt ggttttttat tgaaagaagg aaatgatgct
    42181 ctgaaaaaga aaatcgatgg cgtattaaaa gagatgaaag aagatgggac gacaaagaaa
    42241 atatctgaaa aatattttca aactgatgtt tcagtcaaac cgaaagaaac gatggctgag
    42301 tcggtgagtg agtgatgtac tttcctctaa ttgatttttc cctcatgtgg acttcacttc
    42361 ctttcgtact gcaaggcttg gtatacacat tagggattgg cttcgtgtcg tttcttttag
    42421 gaaatctggt agggttgctt ttgacagttt taggtctttt ggattggctg cctctgaatg
    42481 tatttattcg tttttattta tcgttcttta gaggcattcc cgcactagtc ctgctgtttc
    42541 tgttatattt tgggttacct tatcaattaa gtgccttaac ggcttcagtc atctgcttta
    42601 cgattaccag cagtgcattt attggtgaaa tctatcgagg atcactagca ggcgtttctt
    42661 caggtcagtg ggacgctggt ttagcttctg gtttttcttt ctttggggta ttgaaatata
    42721 tcgttttgcc gcaagctttt cgaatctcag taccggcact cagtaacgta gctatggatc
    42781 tagtgaaagg tacttcgtta gcagcaacaa tcacagtgcc agaaatattt caaaaagcaa
    42841 agatcatagg cggtcggaca ttcgattatt tttctctcta tattttagtt gcactgattt
    42901 attgggtcat ttgtatcggg atcgaaaaag tgcaacagta tttagaaaaa cgttctatgc
    42961 atcagtcaat ctgaaaaata gaaatggtgc tagcgtggct ggcaccattt ctattttttg
    43021 tttcttgtat attctgccta gtattttaaa accatttcat gtaaaatgta aatcgaaaat
    43081 atgttcggag gtagaaaaca tgaagacccc gctaaccatc gaaatagaat cctcgttata
    43141 ccaatattgc atcgagcaag gcggcttggt tgtagaagaa gtcacgatgc ctgatgacca
    43201 aggaattgta gatacgctta gctgcttgac aaaatttgat ggaagccgag aatggcgatg
    43261 ctatgagctg aaagtgacaa aagcagattt tcgctctaca gccaaactgt catttgttgg
    43321 acattataat tattttgttt taccaagtaa actatatgaa gaagtaaaaa ctgaaatccc
    43381 tacaggaata ggtgttttag tgtatcgtcc gtacacggtt gaagaggaga tgcctgcctc
    43441 tggtacattt ctgattgcga aaaaagcagc aaagaaagag cttgctgtac cggaagaagc
    43501 tttgacaaac cgcttcatgg cttctttatt cagggaagta agaaaagcaa agcaaatgga
    43561 gcgaggcgta aaatattttc caacagatca attgtacaaa gaattgaaaa gacgttcaga
    43621 aaatcaagat ccattttcta acgaacgata ttatgaacgg tttttagagg aaatagctag
    43681 tcaggcaatc aatgagttgc aagaagaagt tatcgcgctt cagcatgatt atgaatattt
    43741 gaggcaacaa cagcagatcc gtcggttgcc tacagagcca ttggagtgaa aaatatgtac
    43801 tatccttatt taagaggaaa acaatttgac ctattagcat taaaagaagc actgtcgcga
    43861 ggattgctct ccaataaaat ccaaccagtg atcgaaccag tacgtgattc cgctacgctt
    43921 aaaaatgtga tcgaattatt tcaaaaaaag caccatccaa tcgctgtgat ccaaaatccc
    43981 caagtaggac aattcaaatt attcgatcag catgtgcaca gttggacagt caaagaaaat
    44041 agctctgtcg tccctgcaca gatcttaacg ccagaaaatc tcagtgaagt actggattca
    44101 cctccagcgt ttcttgtttt cgatggacag cactacccaa aagatacaga ggtgtggaaa
    44161 cagttggcag gagttgattc gaaatttttg ataccagata ctagtcgctt tcgcatctgg
    44221 ctgcccgaaa acaaaattgt gatccgtgac tcttttcaaa cgagaaagca tgttgaaagt
    44281 tatgcagata aaacagatga cttttttagt gatgactatc tttttttcca cgcagacgga
    44341 tatattggat tttctgattt cacgatcgaa ggtagccgtt atttcgataa agggttccct
    44401 agccgagcgc ttgctatcca tctaacgtat attgatgctt acggcaatat acgcgtgaaa
    44461 cattttgtct ctgacagtaa cgactctgct aaagaccaag cattgaagtt tcttgaagca
    44521 ggagataaaa tgaaaaaatg gatcatgcgt caccatcatc aactcttgat tacttcagga
    44581 atgattgaat tattggctct ttaccagcaa caaaaattcc ctggactggg agtgttgaaa
    44641 aagtggtcac ttatgcatca tttggaactt attagtcacc tgctagatca tccaaaagat
    44701 tggcttaaat attattcaaa agttcaggaa ctatatgaag tgatccaatg attagataat
    44761 gtaaatgaaa aaggaataga aaagaggaaa ctggatgtat attcggcaaa caaggaaaga
    44821 agaagtagaa gaagtggtag aaatcattaa tgccgcagcg gatttcattg cttcgcaagg
    44881 ctctcctcaa tggcaaaacg ggcagaaacc aacaaaagaa acgattttga acgatattga
    44941 aagaaaagaa agctatgtat taatagtgga agatcaaatt gctggaacag ctgcattagt
    45001 ctcaagcgtg gatcccgttt acacaaaaat agatggtagt tggaaagaat caactggtcc
    45061 ttatttatcg attcatcgtg tcgctttagc agaaagtact cgaggaaaaa aactagcaaa
    45121 accgttctta gaacatttgt tgacgattgc agccagcaaa aatatccaag atatccgtat
    45181 cgatacttat ccagctaatg aaccgatgga aaagaccatc tattctgctg gattcactta
    45241 tcaagggatg attgaatttc cttttgaaca cggcgaaaga aaagcatatc aaataatcat
    45301 caattgacta taaaatcatc tgagcaaaga taaagacatt aacactactg aaaaaaacaa
    45361 tcatatgata gccaagaggt tgtaacagaa gtggccagct tcaaaaaaca agaaagaatt
    45421 tccgaaaatg cttctcaaat ttttgtggaa ttttagcgta ttttacgaaa tagctgcttt
    45481 ctgaaactcc cttcatcgag aggtcgtgac atagcttttg tcacgacctc tcggcttatc
    45541 ctacgagtat tattcgatac atttatatca ataatccttt ttttatttta tctgcagctt
    45601 cttggaacgc tccatcgttt gacacgacta aaagtgaggg gatagctttc aaacgtttca
    45661 actgtgctaa atcttctaca taaaccgcca gtctggctga aatctgttca gatgaatctc
    45721 cacgtttttt caggcgcgat tgcaatgttt ctttagagat cgtaataaag ataggacgga
    45781 ctaccgtccc gaaatactca tataaatgcc aaaaaccatt tgctgtcaaa acggcgtaac
    45841 aatttcctac tgtcagtttc tttttaattt ctgctttact ggaaccatat ttatgttgag
    45901 catattggtc ccactctgca aactcatttc ccttgatcat ctgattgaat gtattgtcag
    45961 aaacaaaata ataatcctct tgatcttttt ccccaggtct tggtgatcga ttcgtgtaag
    46021 taatgatctt ttgttccggc tgaaaaatag cttctgcgag tgaagttttt ccagaaccag
    46081 aaggaccgat aaatacatag caataattca ttgtcatttc tcctttgatg attgaacaag
    46141 tgaaaaagct ttggaaaata tttccaaagc ttttaaaagt aactttgctt ttttgtatat
    46201 tataccagca agccagtcac aatagcactt aaaaagctga ctaaagtcgc tgttagcaaa
    46261 attttcaatc cgtgttttgc gacaagattt cctttttctt catttaatcc cttcatcgca
    46321 ccagaaatga tcccgataga agagaagttg gcaaatgaaa cgaggaagac agatataatt
    46381 gctgttgtac gctcagtaaa atggaaatta ccatttgcta attctgtcat agcaacaaat
    46441 tcatttgtaa ccagtttagt cgccatgata ctaccggcat ccactgcttc tttccatggg
    46501 attccgctga tgaaggcaag aggggcaaag atgtaaccta ggatctcttg gaaagtgatg
    46561 ccgaatatcc cgtggaagat agcattgatc atggcaatca aagcaacaaa accgataagc
    46621 atagcagcaa ccgtgatcgc cacatggaaa ccatcaagaa tgtattctcc caacatttgg
    46681 aaaaatgtct gttctttgtt ttcttcaatg atcagctcat cttctttttc ttctaataca
    46741 tagggattta caattgaagc cagaatataa ccgccaaata agttcaaaac aagggcagtg
    46801 atcacatatt tagaatcgat cagtgccata taagatccga cgatcgacat agaaactgta
    46861 gacatagccg atacactcat ggtgaacaaa cgtttctcag ataggaaggg taattccttt
    46921 tttatggaaa taaaaacttc cgattgtcct aaaatagcag aagctactcc attataagat
    46981 tccaatttgc ccatcccgtt gattttactt aatcctagac caacagcttt cataaataat
    47041 ggaagaatct tgatatagcg tagaataccg ataatagcag aaataaatac aattggcatc
    47101 aatacggaaa gaaagaaagg tgcacttcct ttatttgcta caccggcaaa aacaaagtca
    47161 acaccttgcc cagcaaaagc aagaagatga tcaaacattt ttgccaatac agaaacaacc
    47221 gctgtaccaa aagttgtacg taataatacg aaaccaagaa caagctgtaa aacgaacatc
    47281 acaattagtg gtttcaattt gattgctttt ttgttactgc tgatcaaaaa agcaagccct
    47341 aatgtgacaa ataaaccaat aatacctaat aaataactca aataatcact cctattccaa
    47401 attccgtttt attttacctg ttttgcaaat aaaatactaa tctttagtga aattttcatt
    47461 acttttttag agtgaaacgc attcattttc cattagaaaa taaagcgaac aaaataaaag
    47521 gtttaaaaag tgtaacgtct tagtaaagtc tataaaaaaa cgaaactatc gattaagcta
    47581 atagtttcgt tttcaacatt tataagtaaa ctttttctat tggtaatata gattttcgga
    47641 agggtagaca ggtgcgcagg taatatcgat tcctagtttt ttcaatgttt gttcatcatt
    47701 tttattgacc atcacagtag agtgggcttg tgctccctca agttcagcta gtttatcata
    47761 agcaacttga gcgattggat tcgttactgc actgattgat aaagcgatca agacttcatt
    47821 gatgcttagt gcatccaaag aggaatgaag tgtctttgat ttcaatcctt gtattgtttc
    47881 aagaactaat ggtgacaata aaggaatgtc atctgcgata tgggctaaat gttttactgc
    47941 atttaagata actgccgctg acgcatccat caatgaactt gtacgcccgg taatgatgac
    48001 ttcatctggt aattcgattg caatcacagc agaaggatct tttgtaggac tttgcgcttg
    48061 aatcttatct gcatagtcat gggcagggac taccacgttt cggtcttcag gacgtaaacc
    48121 gacttcttcc ataatcagtt tgattcgatt aactgcttct tcgtcgatca atcctttttt
    48181 gaagtcgcat tctgtttgga aataacgacg gatgatttct tgtttagagg cttcttggac
    48241 aacggtatca tccacgatcc caaatcctac acgattcaca cccatgtcag taggagattg
    48301 ataaacagat tctttaccag taattttttc aataatacgt ttgattacag gaaatgtctc
    48361 aacatcccga ttatagttca cagctacttt attgtaggca tcaaagtgga aggaatcgat
    48421 catattgaca tcttttagat cgactgtggc tgcttcataa gcaatgttca gcggatgttt
    48481 caacggaaca ttccatactg ggaaagtttc gaacttggag taaccagctg catgtccttt
    48541 ttggctttca tgatacagtt ggttcaagga agtagcgagc tttccacttc ctgggccagg
    48601 agctgtcact acaacgattg gtttagttgt ttcaatatac tcattttttc caaaaccatc
    48661 ttctgagacg attttttcta cgttgactgg atagtcttcg atttctgagt gtttatagac
    48721 cttgatgttt cgtctttcta gtttattgat aaagactttc gtggcaggct gtccactata
    48781 gcgagtgata acgacactgt tgattaataa gccataacca cgtaattcat cgattaaccg
    48841 caagatatcc atatcataag taatcccata atcaccacgg atcttattac gttcaatatc
    48901 tccagcatag acgcaaatca gtatttcagc ttgatctttc aatttttgca gcaatttgat
    48961 tttcgcatct tcttcaaatc ctggaagtac acgtttcgca tgcttgtcgt caactagctt
    49021 cccaccaaat tctaaatata atttgtcata gtggttcact cgttccaaaa tatacgctga
    49081 ctgctcttca atatattttt ctgaatcaaa accaattttt ttcactaggt tccctcctta
    49141 agtactaaag cattttttca tttttttgag gattcgtcaa ctggaagagc gaaattcctc
    49201 aaaaaaacaa agaaaatggt ttaaaccaac ttgtctagat agttattcat tggtatgata
    49261 gtttttgata aggaaggtgc tacatgaaaa aaagacagaa aaagaaaaac gcatataaac
    49321 actatatccg ttcgattttt accggttatg aaagaatgct tgaagatccc gaattggaac
    49381 aattgacgtt tacttatcta aacgaagaaa cgcagctaac tcgtgatgag catcagcgca
    49441 ttcatttcac aacaagagat ctgcctagta aataaaaaat aagataaagg tcatcggatt
    49501 cgattctgtc ctatcagatg gaaagactag ttgatcgaaa aaaaggctgt accaaatttt
    49561 ggtgcagcct gttttatgga gcattgcccg ctctttttta taacaatttt ttacagaaaa
    49621 tggtactatt gaagtattaa taagtgaaaa acagatgagt aagaggaggc aagtagtatg
    49681 aaagagccag atacaaatgc aaattatgag ccagacgaac tagaggagac gaatgcggat
    49741 caggatacag gacaagaatc gtcttctttt tacgatatca aagaaaaagc aagcatgaat
    49801 ctttcccaat tattagaaga cttacaggct tttgaaaaag ctgtccgcga agaagatatc
    49861 cctgagatct accgtttata caatggccga ttgaatgaag aattaaaaag aagctcaaat
    49921 gaaaatcacg agatcgatca attgctgatg cgtaagatcc atgatcgttt tttacaggaa
    49981 tttcctttta tggaacaagt agaaaacatc tcgccaacga tgagctatta caaaatcggc
    50041 acttattacc atgatcggcc cacggtcggt attgatgcaa gtctgccaga aatttttgtt
    50101 ttgcctaaga tcgatgaaga gtgggagaaa tattcccaac cggagtcaac ggactatgac
    50161 aaaaaaatca atgagctaga cgcaaaggtg atcactgctc agacagaaat tgaacgttta
    50221 gatgaacaaa taaaagaaat caatcgacaa atgactgatt tgaacgacaa taaaggattc
    50281 ttgaatcgta aaaaaatcga ggaagaaatt caagaattag agaagaagaa gcaagtacta
    50341 tctaatgaaa aattaggctg gcttccttat attgagacgc ctgaaacaat ccagcagcaa
    50401 aaagaagcat tgaagcaaga agcaagagca gaccagcttc gtgcagccat cgtagaaaaa
    50461 gaacaaaggc aaatcagacg ctatttcggt agcaaagagg gattcggtca agcaatccat
    50521 gaattcttga tgaattatct tggcaatgag aatcctaata atcaatctaa tgaaggaggt
    50581 tccgagtatg aatgaacacg atgaagcaga atggacaaaa aatgaagaac aaacagaaga
    50641 tcatgaagaa acgaacactt cattggaaga acaaacaagt aatcctctgg atgacacaga
    50701 agaaatccct atgctagagg aaacaaaaat acacgaaaaa gaaaaagtaa ctccgacgaa
    50761 tacgaatact gcagaaaaca cccatgctta cgaaataact gaaaatcccg ggtatttaga
    50821 aggaaaagag caagatcatc aagatatcgt cagtttgatt gaaacagcgg aagcagaatt
    50881 agcggattta cgtatgagaa aagatttgat cgaatcagat tttccacaat tattagaatc
    50941 atatgatcgt tttatattga atgaagaacc aatctccacg atcggaaaga caagcctgat
    51001 cgaatacttc acgtatgaat tactgaaacc gttgaatgat attggtctgg aacggtcaaa
    51061 ttcagcttat gacacatgga aaacaagaca tttttcgatc aattatacgt tgaatggaca
    51121 aaacgaatta gtcttttcct ttgtattacc gaccatcgat caacgatacc aagtggaatc
    51181 gatgagtctc ctaaacgtat caccagagac aatggaaatc aatgtccaag atgaccgggt
    51241 gctttctttg atccgacact ggtcagtgga tcgagtattc tctgcagggc agatcacgat
    51301 attcaatcac aaattgaatc aattattagc ccatgcaaga acacttggtt ttaccgttaa
    51361 tcagacatta ctagacaata cgaagccact tcgtttgaat cttcagagcg aatttgaatt
    51421 gacgaatgaa gtattggatg atatttttat cgtggcaatg aaaaatccat cctacgatat
    51481 ggaaaagatc aacgaaacta cttatcaagt acgattagat aaaggacagt ctttaacgat
    51541 aacaacaaac gaaaaagatc aaacagtctt gtcgatctca tctggagact acccacgatc
    51601 tgtcattgat ttctttatca attacgaatt cttggtccca ttaatggtga gaaagtcctg
    51661 attggataag ttggattacc agatgtcatt cagataattc tgttccaaag gacttgcggc
    51721 agttatccag aataattgtc acaagtcttt tctgtatgta ctaaatggac gtgaataact
    51781 tgctgatgaa ggagggattt cattaggaat gtccacaaat aaaatagcac agcttagctt
    51841 attgtctgct gcttgtgtag caggtaggat cgcttttcaa tttattccga atttccagcc
    51901 aatgactgct atttttttat ttatagtact ttacttaagc ttaaaagatg cgttaattgt
    51961 catgagtctt tctattgcga tcagcagttt ttattttggt gttggaccat gggtcatcgg
    52021 ccaatggatc agctatactg ttgttctgag tttgttttca atcgtctgct tgagaaaaac
    52081 agtgcaaagg aatctatggt taaagggagt ctgttttttt ctggcgggca tggtttacgg
    52141 aataggtatg accgtttttg atacgctgtt atatcgtttg cctcaacctt ggatctatta
    52201 ccttcaaggg gtgtcgtttg atttgatgca cagtataggc aatgttgtgt tctttcttgt
    52261 ttttctgcca gtcgtcaaac gtttttacaa tcattcagga ggaaaaaatg aaaaaaataa
    52321 tttatagttt gcttgcaatg agtgtcagta tactcatttt gacaagttgc agtacaacct
    52381 atacacaatc ggcttccaac caaaaggagg aagtaaaaac atcgcaaaaa gcaacaatca
    52441 cgttacaaga aaatggtcaa aacttttcgg aaaaagaagt gttcttcaaa aaaggcgatg
    52501 atttactgag tattttgaaa cgaaattttg aagtcaaaga agataacggc ttcatcactt
    52561 cgcttgaagg gcactcacag gatgagaaaa atcaactcta ttggatgttt actgtggacg
    52621 ggaaaagtgc tactactggt gccaaagaga tcaaattgaa tgatggacaa tcggtcattt
    52681 tcaatttgtc taaactataa gtttcataaa aaactgttaa agggcgaata ttgccataat
    52741 tcagtgagtt cagttgatga aagaaggaaa ataacatgga aaattttaaa aaaagcggaa
    52801 tgttttccgt aattgctgct ctaggggcta atattctagt tgctatcagc aaatttgtcg
    52861 gctatgcaat cagtggcagt gccgccatgc taaacgaaag catccacagt atcgttgatt
    52921 gcagcaatca gattttgctg ttattcggtg ataaacaagc caataaagga cagagcgagt
    52981 tgcatcaatt tggtgaagga cgagcaaaat actttttcag tacaatcgtc gcaatgatgc
    53041 ttttcttcgg cggtggggca cttggggtga tggaagcttt tgaaaagttg acgcatccat
    53101 cccatgaagt gggtaatccg atcattgtta tagcaatact gttatttggt ttggtaatcg
    53161 aaggaagttc gttacgcgtg gcaatgaaag aaatcaaaga attgaatact gaaaaacttt
    53221 ccacgatgcg ctttttgcgc gaaagtcgac acagtgaaat actgattatt tttacggaag
    53281 acttgtgtgc tgtaatcggt ttggtcttgg ctttagcagg aacaatattg acttatgcaa
    53341 ccgggattgc tgcatttgat gcgatcagcg gattgctgat cggtttcttg ctgatgggag
    53401 ccgccatttt cctagcaaaa gaattttata gtctgatcat cggtgaaagc gtgacagcaa
    53461 gcgatttatc aaaaatcaag ttagcttttg aacgtcctga gatcagtcgg ctgatcgata
    53521 taaaaacagt tcatttaagt ccaacggaga ttttagttgc agcaaaagca gatatcgttg
    53581 gtaacaaaga gaatgaagct tactcggtca tcaatgatat tgaaaaaaat atgcgtcagt
    53641 ctcttcctga caagaagatg tatatataca ttgaaacaga taaatatgac ccaaattatt
    53701 ctcgtaaata aaacgtttat tcatgaattg aagcaaaaat gagaaaaata taatcttgtt
    53761 tttaaataaa acggctacaa tttagcgaat ttatgttaca atgagtccgc tgtttatttg
    53821 ttgtaattaa aggagatatt ttaatgaaca aaaagaaaag tgcgccatgg cttcaagctt
    53881 tgcatgcagt gatgccactt tgtataagct atataccagt aggattagct tgcggagtat
    53941 tgttgcaaaa agttggattt gatcctctct tatcaggact gttatcgatt ctggtctttt
    54001 ccggtggcgc gcagttttta gttgcatcta tgcttacgac gcaagcatca tttgcaacga
    54061 cattattgat ggttttcttt ttagaattac gctatacatt attaggagca agtcttgccg
    54121 gttttatgaa aaaagaaaag cggacgtttt tggctgtctt ttctcaatca ctgaatgatg
    54181 aaaactatgc ggtgaactac ttaaagtttt caactgaccc ttcctggaat aaaagaaagg
    54241 ctttatatgt caattggttc tcaatgactg catgggccgg tagcaatatg atcggtaatt
    54301 ttttcggctc ggtgattcga gtagatgctg accttgtcca ttttgctttg acggccatgt
    54361 tcattttcat gtttatcatg cagatgaaaa gcgcactact gatctttaca ggtttattct
    54421 caggcgtatt aggggtcgta ttcatggtat tattccaaaa tactttcgga ttgattgcag
    54481 caacagtcat tgcttcattt gccggattta tgctggagaa ggcattcaaa aaagaaaaag
    54541 ctaagaaact aagagaccgc ggtaaagatg tcaatgagcc gctcttcaac ttcccggaac
    54601 aggaggccaa tcaccatgac taaactatat ttgctacttt tagttgcttg tctgttcatc
    54661 gtggcatata tcccgcgctt gttcccgatg ctttatttta ctcatcgaaa agtgccatcc
    54721 tggttcagtg actggatgaa atatgtaccg gtggcattat ttgcggcgtt agcttttaaa
    54781 gatgttttca ttactcatga acatctggat attgcttgga atatcaaaat agctgcaatg
    54841 attctagtag caggcgtggc ttacaaaaca cgttcgatgg cgttgtcagt tttgacagga
    54901 cttgcgtctg tctttttgct gtctatgctt taaactgttt tattttatta aaaacagaga
    54961 ttgcgacata agttgttcaa actgatctat ccgaactgca tttgcaacag tcaattctgg
    55021 atgcaagttt attcgtaggg ataaaaaaga ttagtgttgg cttatgatgc aagctctttt
    55081 ttattttagg aattagagag ggatttgtcc ttctatgatt ttttgctata atagggaaga
    55141 tgtttatttt atgaaagaat agttgaggga atttaaatga ataaaaaatt gatgcaagga
    55201 acattttggc taacctttgc taatcttcta tgtaaagttt taggggtagt ctatttgatt
    55261 ccctggctaa gtatgatggg gaacaatcaa gatggtatgc ttgctacgac attgtataat
    55321 gttggctatc ttccttatgg attgttcctg atgcttggta cagtcggatt tccaaatgcg
    55381 attgcaaaaa aagtagctgt agccacaaaa gaaggagata acgctgcttg tcggctgatt
    55441 tttagaagta cgatcaatat catgtttgtc atcggtatcg cctctgctgc actgatgtat
    55501 ttatttgctc ctttactagc cggaatcagt ccgattgcta acgttaataa tgggatacta
    55561 gctatcagaa gtttatgccc atctttgatt gctatcccaa ttcttagtgc aatgagagga
    55621 tatttccaag gaaaaaatga tctgcggcct tatggaactt ctttgattat cgaacaggct
    55681 gtgcgagtga tcgttatctt ggctggtact tattacttgc gcgtattgac tgacggaacc
    55741 atattagaag ctgtattgat cagtacagtc gcttctttct tcggtgggtt agctgcgatt
    55801 atccatatgt atatcgtcgg acgcagaaaa gacttcttcg aacttaaaga ttttttgatt
    55861 tccagccgtt atttcgaaag agaaaaccga tctgcctctg tctctatcat tcgggaaaca
    55921 ctaccgttta tttttgtcgg ctcagtcatt actatcgttc aaatgatcga ccaagtaacg
    55981 atgaaaccac tgcttcactt tttccgtcca gagatcgcag atcagcaact ggaattctta
    56041 ttcagccgtg cttctgtcaa tccaaataaa ttgacattga tcctgatctc catggtagga
    56101 acagtagcta taagcagctt gccaatatta agcacgatga agaaaaaaga tcggatacaa
    56161 atcgaaaaaa cagtaggaga tagtttttcg attgcgttat tgattcttct tccttcattg
    56221 actggaatgt ctttattagc tggaccacta tacacattgt tttttggcta tgatcctgaa
    56281 agtgtcggat atttccaaat ggctttgcta gcttctttgt ttttctcttt attcacgatc
    56341 ctatctacga tgcttcaatc tttgaatcac catcgttttg cgatccgatt aactgtagaa
    56401 gcaattatcc tgaaagtcat cttccaagta atcggattag gattagttgg cggatacggg
    56461 atgagcttat caaatggctt agcattcggt attgtctttg ttcgcggcta tcactttatg
    56521 tgcaaagaat accgaatagc acctttagca aagatcagca attttttctt aaaaactttc
    56581 cgctccaccc tgattatgct tgcagtatgt tttgccgttt tctttttgct gaatcagcga
    56641 ttgtcaatgg aatcaaaaag ctatgcaatg atttattgtg tggctgtagg aagtattggc
    56701 ggcttagtat ttttattttc tcaattcgga aaaaatgggt ttcgaatgtt gaaaaatgtt
    56761 aaacatcata gcagaagcaa agaataggtt aactaatcgt tttttgctgt cacttgaaaa
    56821 acggcaactc gtatgattac gcttgtcgtt tttctatttt ccaattttag tttcatatct
    56881 aatcttactt atgatatagt tataaggtct ggaaaaattc tttgaaacga ggaaattaaa
    56941 cacatgaaac ttgcagtagt tacagatagt tcggcatatc tgccggaacg tatcaagaac
    57001 catcccgact tattcgttat tccaatccct ttgattatgg atgggaaaat ttataacgaa
    57061 ggaatcgata tcgaagcgga tgaatattat ggacttttaa aaaacagtaa agattttcct
    57121 actacttcac aacctgtcgt aggggaagtt ttgggtttat atgaggaatt gaaagagaaa
    57181 gggtatgaca cgattttaag tatccatctt tcttcaggga tttctggctt tgtcaacaca
    57241 ttatttgcga tgaaagatga catcagcgga gtaacggtca ttccttatga ctctaagatc
    57301 actagtatgc cgatgggaca catggtagaa gctgccttag acttaaatga tgaaggtcag
    57361 tcccttgatg aaatacttgc acatatcgat cgaatccgag ataataccta tgcttatctg
    57421 attgtagatg atttgaacaa tttagtacga ggaggacgtt tgacaaatgg tgcagcgctg
    57481 attggcagct tattaaaaat caaaccgatc ctgacatttt ctgaagggaa aattgtttta
    57541 ttcgaaaaaa tccgttcaag taaaaaagca tttgctcgag ctgaagaaat tattggtaga
    57601 agaaataacg aaatacaacc gccagtcaaa ctttacgtga tacatgcaaa taatttagaa
    57661 gtagcgattg cggagaaaga gaaattgcag aaaaaatatc caaatgcaga aatcgaaatt
    57721 ggtcatttcg gaccagtcat tggtacacat ttaggtgaaa aagccatcgg cattgccatt
    57781 tcagcgcaat aattcctttt attcaaaaaa cataccaaaa at
//
LOCUS       DACECR010000005        57556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.005, whole genome shotgun sequence.
ACCESSION   DACECR010000005 DACECR010000000
VERSION     DACECR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.005"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..816
                     /locus_tag="IWO17_000263"
     CDS             4..816
                     /locus_tag="IWO17_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ0289165.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IWO17_000264"
     CDS             813..1613
                     /locus_tag="IWO17_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ0289166.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IWO17_000265"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IWO17_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ0289167.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IWO17_000266"
     CDS             3110..4819
                     /locus_tag="IWO17_000266"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ0289168.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="IWO17_000267"
     CDS             5031..9383
                     /locus_tag="IWO17_000267"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ0289169.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="IWO17_000268"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="IWO17_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ0289170.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="IWO17_000269"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="IWO17_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ0289171.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="IWO17_000270"
     CDS             11221..11514
                     /locus_tag="IWO17_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ0289172.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="IWO17_000271"
     CDS             11511..11822
                     /locus_tag="IWO17_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ0289173.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="IWO17_000272"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="IWO17_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ0289174.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="IWO17_000273"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="IWO17_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ0289175.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="IWO17_000274"
     CDS             complement(14623..14844)
                     /locus_tag="IWO17_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0289176.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17655
                     /locus_tag="IWO17_000275"
     CDS             15292..17655
                     /locus_tag="IWO17_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAQ0289177.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAKKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18023..18946
                     /gene="truB"
                     /locus_tag="IWO17_000276"
     CDS             18023..18946
                     /gene="truB"
                     /locus_tag="IWO17_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ0289178.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18950..19891
                     /gene="ribF"
                     /locus_tag="IWO17_000277"
     CDS             18950..19891
                     /gene="ribF"
                     /locus_tag="IWO17_000277"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ0289179.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20243..21028
                     /locus_tag="IWO17_000278"
     CDS             20243..21028
                     /locus_tag="IWO17_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0289180.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21041..21892
                     /locus_tag="IWO17_000279"
     CDS             21041..21892
                     /locus_tag="IWO17_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ0289181.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22016..22468)
                     /locus_tag="IWO17_000280"
     CDS             complement(22016..22468)
                     /locus_tag="IWO17_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0289182.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22933..23301)
                     /locus_tag="IWO17_000281"
     CDS             complement(22933..23301)
                     /locus_tag="IWO17_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ0289183.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23451..23933
                     /locus_tag="IWO17_000282"
     CDS             23451..23933
                     /locus_tag="IWO17_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ0289184.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            23970..24980
                     /locus_tag="IWO17_000283"
     CDS             23970..24980
                     /locus_tag="IWO17_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ0289185.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25095..25808)
                     /locus_tag="IWO17_000284"
     CDS             complement(25095..25808)
                     /locus_tag="IWO17_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289186.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(25998..26588)
                     /locus_tag="IWO17_000285"
     CDS             complement(25998..26588)
                     /locus_tag="IWO17_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ0289187.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27072..27299
                     /locus_tag="IWO17_000286"
     CDS             27072..27299
                     /locus_tag="IWO17_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ0289188.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27315..28481
                     /locus_tag="IWO17_000287"
     CDS             27315..28481
                     /locus_tag="IWO17_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ0289189.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28715..29758
                     /gene="hrcA"
                     /locus_tag="IWO17_000288"
     CDS             28715..29758
                     /gene="hrcA"
                     /locus_tag="IWO17_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ0289190.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29852..30415
                     /gene="grpE"
                     /locus_tag="IWO17_000289"
     CDS             29852..30415
                     /gene="grpE"
                     /locus_tag="IWO17_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ0289191.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30466..32295
                     /gene="dnaK"
                     /locus_tag="IWO17_000290"
     CDS             30466..32295
                     /gene="dnaK"
                     /locus_tag="IWO17_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ0289192.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32446..33612
                     /gene="dnaJ"
                     /locus_tag="IWO17_000291"
     CDS             32446..33612
                     /gene="dnaJ"
                     /locus_tag="IWO17_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ0289193.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(33694..34359)
                     /locus_tag="IWO17_000292"
     CDS             complement(33694..34359)
                     /locus_tag="IWO17_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ0289194.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            34335..34508
                     /locus_tag="IWO17_000293"
     CDS             34335..34508
                     /locus_tag="IWO17_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0289195.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(34736..34942)
                     /locus_tag="IWO17_000294"
     CDS             complement(34736..34942)
                     /locus_tag="IWO17_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0289196.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(34968..35387)
                     /locus_tag="IWO17_000295"
     CDS             complement(34968..35387)
                     /locus_tag="IWO17_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0289197.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            35891..36244
                     /locus_tag="IWO17_000296"
     CDS             35891..36244
                     /locus_tag="IWO17_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0289198.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            complement(36304..36471)
                     /locus_tag="IWO17_000297"
     CDS             complement(36304..36471)
                     /locus_tag="IWO17_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0289199.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(36590..38509)
                     /gene="tet(M)"
                     /locus_tag="IWO17_000298"
     CDS             complement(36590..38509)
                     /gene="tet(M)"
                     /locus_tag="IWO17_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0289200.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            complement(38525..38641)
                     /locus_tag="IWO17_000299"
     CDS             complement(38525..38641)
                     /locus_tag="IWO17_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0289201.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(38886..38963)
                     /locus_tag="IWO17_000300"
                     /pseudo
     CDS             complement(38886..38963)
                     /locus_tag="IWO17_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(39117..39752)
                     /locus_tag="IWO17_000301"
     CDS             complement(39117..39752)
                     /locus_tag="IWO17_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0289202.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            40264..41406
                     /locus_tag="IWO17_000302"
     CDS             40264..41406
                     /locus_tag="IWO17_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0289203.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            41399..41605
                     /locus_tag="IWO17_000303"
     CDS             41399..41605
                     /locus_tag="IWO17_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAQ0289204.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            41684..42877
                     /locus_tag="IWO17_000304"
     CDS             41684..42877
                     /locus_tag="IWO17_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0289205.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(42884..43042)
                     /locus_tag="IWO17_000305"
                     /pseudo
     CDS             complement(42884..43042)
                     /locus_tag="IWO17_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
     gene            complement(43048..43785)
                     /locus_tag="IWO17_000306"
     CDS             complement(43048..43785)
                     /locus_tag="IWO17_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0289206.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(43787..45289)
                     /locus_tag="IWO17_000307"
     CDS             complement(43787..45289)
                     /locus_tag="IWO17_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0289207.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            45548..47542
                     /gene="uvrB"
                     /locus_tag="IWO17_000308"
     CDS             45548..47542
                     /gene="uvrB"
                     /locus_tag="IWO17_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ0289208.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            47552..50368
                     /gene="uvrA"
                     /locus_tag="IWO17_000309"
     CDS             47552..50368
                     /gene="uvrA"
                     /locus_tag="IWO17_000309"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ0289209.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            50631..51896
                     /locus_tag="IWO17_000310"
     CDS             50631..51896
                     /locus_tag="IWO17_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ0289210.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            51900..52631
                     /locus_tag="IWO17_000311"
     CDS             51900..52631
                     /locus_tag="IWO17_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ0289211.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            52766..53650
                     /gene="rapZ"
                     /locus_tag="IWO17_000312"
     CDS             52766..53650
                     /gene="rapZ"
                     /locus_tag="IWO17_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ0289212.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            53647..54645
                     /locus_tag="IWO17_000313"
     CDS             53647..54645
                     /locus_tag="IWO17_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ0289213.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            54679..55614
                     /gene="whiA"
                     /locus_tag="IWO17_000314"
     CDS             54679..55614
                     /gene="whiA"
                     /locus_tag="IWO17_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ0289214.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            56157..56807
                     /locus_tag="IWO17_000315"
     CDS             56157..56807
                     /locus_tag="IWO17_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289215.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            56994..>57556
                     /locus_tag="IWO17_000316"
     CDS             56994..>57556
                     /locus_tag="IWO17_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ0289216.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        19160 a        10055 c        12049 g        16292 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac taggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccactcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg aaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgacc aataaaatga taaaatcgat cccgcaaaat agaatttttt acattgatta
    17701 agaaactgaa aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg ccagtctttt
    17761 ttttcgtttt tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat
    17821 caaatggtgc aaagaaaaaa tcttccattc tattccctcc tttaaaaaag cataacatga
    17881 tttctattta cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt
    17941 cattatgatg tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc
    18001 aaaaatgaaa tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat
    18061 gacaagtcat gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca
    18121 tgggggtacg cttgacccag acgtagacgg tgtgttgccc atttgtattg gaaaagccac
    18181 aaaagttatc gaatatttga cagattctgg gaaaatctat gtaggcgaga taactctagg
    18241 tttcagtaca acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat
    18301 gtctttatct gaaaaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca
    18361 gataccacca atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg
    18421 caataatgaa acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac
    18481 aagtgaattg acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg
    18541 cgggaaagga acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc
    18601 tgcgcacatg tctgatctga cccgaacagc aagtgccggt atgtcccaga aggatgctct
    18661 gacattagaa gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc
    18721 aatagaaaaa ggagtggaac ggttcaaacg agtagatatt actgatgaag tatggcagaa
    18781 agtaaaaaac ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga
    18841 agaaattgct ttgttttata aaggaaaagt tgtcagtatt tatcagccaa acccgaaaga
    18901 aaaaaacaaa ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat
    18961 caagatcaga catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtgct
    19021 gggatttttc gacggcgtgc atatcggaca ccagaaagtc ataaagacag gtatggaaat
    19081 tgcacaaaaa gaaggattga aattagcatt gatgactttc aatcagcatc catctattgt
    19141 ctttaaaaag atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga
    19201 aaaaatggcc gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc
    19261 tcatttagca ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc
    19321 tgtttccggt tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc
    19381 tgaatatgcc aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga
    19441 aaaaatcagc tctacccgta ttcgtggatt actagacagc ggacaagtaa aagaagcagc
    19501 aaaactatta ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg
    19561 cagactacta ggattcccta cagcaaatgt caaagtaaag agcacggttc atttgcctaa
    19621 agaaggtgtt tatgtgtcgg aaataaaaat tggggaaaca tggtatccag ccatgggttc
    19681 aattggacat aatgatacat tcggtgaagg acgggagctg actgtagagt tgtatatttt
    19741 ggactttaat caagatatct atggtgaaca tgtcgcggta cgctggaatc aatttttaag
    19801 agatcaagtg aaattttcta gtgtagagga attgattgaa caactcaagg aggatgaaca
    19861 agcaacagct gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg
    19921 tataactagc aattacaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa
    19981 aagtcttgtc acagcctttt tttccaaata aatggttttt cagaagtagc ttcttcggaa
    20041 ataagccgaa atttcttgaa gctgaccact tctgtcacaa cctctttctt tatagaaata
    20101 aaaggacact agcaattcat gaaaacgatt gctagagcgt atcatttgct ttagactata
    20161 tatcttggtg tacactcact ttaaggcgga aataaaagtt atccgtaata acaggtttag
    20221 gtatgaaaaa ggagtgggta ctatgtacaa ggaattagaa aacaaagtcg cagtagtaac
    20281 tggtggctca aaggggattg gaacagcgat tgctcaacga ttcgggaaag aaaaaatgaa
    20341 tgtagttgtc aactataact cggataaaga aggagcagaa aaagcagtca aagcaatcga
    20401 agaggcaggc ggaaaagcag tctctgtaca agcagatgtc agttctgaag aaggaataca
    20461 gcgtttactt gatacggctt tagaaaattt tgggacactg gacgtttggg ttaataatgc
    20521 tgggatggaa aatcagcatc caacccatga acttagcctt gaagattggg aaagagtgtt
    20581 aaaagtcaat ttgactggcg tttttatcgg aacgaaagcg gctttaaatt actttacaga
    20641 gaataataaa aaaggaaata tcatcaatat gtcttctgtt catgaacaga tcccatggcc
    20701 aacgtttgct cattacgctg cttcaaaagg cggggtaaaa ttatttaccg aaacggtagc
    20761 aatggagtat gcaccgaagg gaatcagaat caatagtata ggccctggtg ctatccgtac
    20821 acctattaat gcggcaaaat ttgatgatcc tgaacaaaaa gaactcttgg aaagtatgat
    20881 cccactagga cgcgtaggtg aaccaaaaga aatagcggcg acagctgcat ggttggcttc
    20941 agacgaatcc agttatgtta caggtattac actatttgca gatggtggga tgactttata
    21001 tccttctttc caaggtggaa aaggataagg gtgaataaaa atgggtttat tgattgcatt
    21061 gatcccggca gttggctggg ggatccagcc actgattttg aaaaagattg gtggcagacc
    21121 aacaaatgag atattaggaa ctggtattgg agcggtgctt gtcggcttac tcgttcaatt
    21181 attcgcttct ccagctggta tttcagtgac tacatttttg attagtttac tttctggtgc
    21241 attttgggtt attggacaaa caggacagta tacatctttc aacttgattg gtgtttctaa
    21301 aacgatgcca atttcgactg cattacagtt agtcggaacc tcgttgattg gtgtcttcgc
    21361 ttttggagaa tggtctggaa gtatggggaa aatcattgga gcagcagcaa tcattcttct
    21421 tgtatttggt tctgctttaa cggcaatcag tgatgatcct ggaaaaaaag gcggtatcac
    21481 aaaaggaatt agtctattag catcaacaag tattggttat tgggtataca gtgcattgcc
    21541 taaactagtt gatgcaagcg gactttccat tttctttccg caaatgatcg gtgtcttttt
    21601 aggggcagtg atttatgtga ttttccgtaa accaaccgca ttttctgaag acaagagttg
    21661 gaaagcaacg atcgttggag ccatctttag tgtgtcagca ttggcttata ttttttcagc
    21721 aaatgaaaat ggggttgcta ctgcatatat cattacacag ctaaacgtag ttatctctac
    21781 attaggcggt atgtacatcc tgcatgaaaa taagacacca aaagaaaaaa aattaacgat
    21841 tatcggatta ttgctgatcg tagtaggaag catggtcacg gtctttattt gaggataaag
    21901 atggtaaaat ttcatttatt aaaaaaatat ttgtttgaac caaacagtaa gtacttataa
    21961 aaaaatagcg ctagaatttc caacatctct ggaatttcta gcgcttttta attgtttaag
    22021 atacaaggga aatatctgtc tggatcaata atttttcatt gatccattga ctaaagttta
    22081 cgacataatt gaagttacca ccgattagta tctccgcatg attgtcttta taaagatcgt
    22141 attgtaaaag acggtctttt ttatttgcgt aaacatcgtt atggtactgg actaattgtt
    22201 ctcggaattg attgattgct gtaaaatcac gatattgaag gttgaaacgc atcgtgattg
    22261 gtgcatttag catgaatcgc tgcatttctt ttgctgcttc ctctgcttga tctccgtata
    22321 ggaactcaaa aagattacgt tcagaaaatt tccattgccc gccaaccttt gttcctttta
    22381 gttttccttc atgaagataa tttctgatag aacgtgtagt aacacctaat tgttcggcta
    22441 attcatttac tgagtaaaaa tctttcaatg ttcaccctcc tatgtgaatg gttctactga
    22501 cttagttgat atttgcttaa tcacgaaatg tagaaagaac gattttaatt aaggtcattg
    22561 taaaaatcaa gtcagaaaat tctaactttt atttctctaa agaaattcgt tctctcctaa
    22621 agtgatatta tatttaaata tacctgaaaa taatgggcag tattatgaaa gatcactgat
    22681 attttctcgt attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt
    22741 ttactaagta gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact
    22801 ccttataaaa atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga
    22861 taggattgaa caaaacctgt ataaggtgat gaatagtttc ctcgccagta aaaggtttat
    22921 tcgtataaag ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga
    22981 aatcaattgt tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt
    23041 atccgtatat acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt
    23101 ttccggtgcg atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg
    23161 gtcagtaaac ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt
    23221 tatcttagca aaaaggtctt tgatcttttg ttgatgttct ttttctttga tttgattgat
    23281 gatcggctga aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc
    23341 agaaaaacaa tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat
    23401 atttatatga taagatgtct caaataataa aatagaaaag agacataaaa atgattgact
    23461 tggcaaagaa attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt
    23521 atacaaatga agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca
    23581 aaagcttatt cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga
    23641 aacaaacgga tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat
    23701 cagaacgcat ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg
    23761 aaaaacagat tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg
    23821 caccaggtag accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc
    23881 agctacttga gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta
    23941 aaatagtgca tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg
    24001 caacgattgg ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg
    24061 aatcagcttt aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt
    24121 ggacgcaaga taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa
    24181 tgatgcaaga tgaaaatatc caaatgatct ttccgacttg gggaggagaa ttattgattg
    24241 agattctcga ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag
    24301 atattagtac gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa
    24361 ctaaccttat tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg
    24421 tactgaaaac aagagaagga gaatcgattc tacagcaatc ttctgaaaag tatcaaaaag
    24481 tgtggcccca tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc
    24541 aatcagtcag cggagatgca gtaaggatga agggacgatt attaggtggt tgtattgata
    24601 cgatcaggca tttaatcggt actccttatg gagatgtcca atcttttaaa agaaagtata
    24661 atgatggaaa gccgatcttg tggtttctag aaaactgtga gctatcgtca gttgatttaa
    24721 gaagatcact tgttcagatg aaactggcag gctggtttga tggcagctcg gggatcatgt
    24781 tcggtagaag tgcggcagag gatttgttag attataaaat gaaggatgta tacgaagatc
    24841 tgtcaaagga attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac
    24901 aactgacatt gattaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg
    24961 ttttgcaaac gtttatttaa aaaatttttt cctgccttgg agtctatcaa agaccaataa
    25021 atctaaatag aaaagaaagt gaaagaagct gacaaaatgc tgtcagcttc tttttgtata
    25081 acatattaga aagtctaatt atctataact gtcacatcga ttaactgttt cacgtcattg
    25141 ggatttcctg taggaatagc tactacagat gcattatcaa tgatttcttg agttgtttct
    25201 ctaatgaacg ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga
    25261 taactagtga ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat
    25321 ttcccagtga gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg
    25381 ataggtgttc ctgcaggata atgaataaca tgtaattcga aatcacccga tgtatctcta
    25441 aaagtattac ctctaatagc tagccaaaca cgtgcgtact caatttgtag atcagaatat
    25501 cctgctaacg gatctattga ctgagaagac tgtgaggagg ttgtgtctat tgttgaagaa
    25561 gaaatatttg tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta
    25621 ctgcttgttg aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca
    25681 gctgaactgg tttgtctgat tgaacttttg tctgtggtcg cagatggatc actattttct
    25741 ttactaggag acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac
    25801 tttttcatat aaaatacctc attatttttt taataccagt atagcataat cctgaatttt
    25861 tgaatccgta aaaccttgtc tatgaatata aatagagtgt atcttctttg ataaatgtca
    25921 gaaaaagtgt gacaaataat catcagtaaa attacttgtc acactattga taaggagtat
    25981 ttaattgaag agaatgatta ggagaaagga tctccgtact ttttatagta atcatagaat
    26041 ccaagtttgt atagacgaat cgttttttct cttatttctt ctgtttcaaa atggactgct
    26101 tgaagaatca agtctgtgaa ttctatagga gctgttccat tcgcagtaat caaattacga
    26161 tctttcacag cttgttgttc tataaaatct tctttattct gataattttc cgattctaac
    26221 caaggaaatt gcgcatttcc ggtgtgtttg taaccttgca ataatccgct ctgagctaaa
    26281 taatcaactg ccccacaaat agcagctact ggtacgtctg tttttaacaa tttatcgatt
    26341 atttgatata aatgatgatt ttttatgtcc catgaattgc caccgatcat aataaaaagg
    26401 tcaacttttt caggtatttg ttcgattaag taatcgattt ttgtttttaa tcctccaatg
    26461 gaagtgacct ctgattggat agaagcagtt ttgttttgcc atttctcatt ttggtttagt
    26521 gtgctagtaa tataagcacc ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca
    26581 gttttcattt tcaagctctc ctttactatc tataacaagg acaacagttt taacctacag
    26641 acattcatta aaattgggta gcgacagcga aaacaacaat tcttaagttt tagacagagt
    26701 atacagatag tcaacttaat taaatacgaa ctaaataaaa atgacaggta atcatttttg
    26761 tttaccagca ttataacatt ttgcatacat ggatgacaaa agaaccaatt cccacttgct
    26821 tgagtacaat attaaaagaa actggaaggc aaaaagtaat tatagaaacg aaaaataaat
    26881 catatcgggc actcagacat gaaaacaacc ctagagatat ataactaggt aaccagttta
    26941 atgaaagaaa aaatcatcca agaagtcgat tcttggattt tttagtggat ttgttcgcca
    27001 aatacgttta gtatgcgtat atgagaggtt gtaagcaaag cgttgtggtc tgagcactta
    27061 aggaggaatc tgtgaaaata gcttttcata tttttgcaga ttttgactta atgccgaagg
    27121 tcagctttgc gattaccgtt tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca
    27181 ataagcaagc atttttagag ctaggtttcc ttgattctaa atatgcggct tatttcaatg
    27241 agttggtgct tgaacgcgat tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag
    27301 gagaaagaat ttcaatgata gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca
    27361 ttcatatccc attttgcgag catatctgct attactgtga ttttaataaa gtttttttag
    27421 aagggcagcc agtagatgag tatgtggaaa tgctgctgaa tgaaatgaaa ataatgctag
    27481 aaagtcatcc gacagcgaat ttagaaacac tttacgtagg gggcgggaca ccaacttctt
    27541 tatctgctaa acaacttgat cgactgttat caggtgctag agagatcctg ccttttcaaa
    27601 atggtaatga gtttactgta gaagcgaatc caggtgattt gacccgtgaa aaacttcaag
    27661 taatgaaaaa ctatggcgtc aatcgtttat ccatgggagt gcagacattt gataatcgct
    27721 tattgaaaaa aattggacgt aaacacaccg cagaagatgt gtatcaaact atgcaatttt
    27781 tagaagcaga aaattttacc aatgtaagca tcgatttgat ttatgcatta ccgggacaaa
    27841 cactagaagg atatctggat actttggaaa aagctttggc tttggatctg cctcactatt
    27901 ctttgtactc attgatcctc gaaaacaaaa caatgttcat gaactgggta cgacaaggaa
    27961 gactcgaact gcctgatcag gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa
    28021 tggaaaagca tggacgtcac caatatgaaa taagtaactt tgggctctcc ggacatgaat
    28081 ctcaacataa cctcatgtac tggaacaatg accactattt tgggtttgga gctggtgcta
    28141 gcggttatct tggagatatt cgttaccgca acaaaggacc aatccaacac tatttacgcc
    28201 cattacgagc aaatgaacgc ccggtattgg aagaagaaaa attgacacga aaaaatcaaa
    28261 tagaagaaga aatgttctta ggactaagaa aaaaaacagg gattactaaa aaacatttct
    28321 atgatcgcta tcaaaaaaca attgaatcgg tgtacggtga tgtgctaagg gaattagagc
    28381 aagaagggtt acttgttaat gaagaagacc gtgtttattt gacaccaaga gggacctttt
    28441 tggggaacaa tgtgtttgag cgctttttgt ttgaaaaata gtttaaagaa tatttgtttt
    28501 ttatctgaat ttaaataaaa aaagttcaaa aatacaataa aggactattg aaacgttgat
    28561 ataactggat ttacatcaat tatttagaat tagcactctt gtgtttagag tgctaaaaaa
    28621 tggagtcttt ctcttgacag gagaagggct tcttgctata tttataagtg gattagcact
    28681 tagaatatat gagtgctaat gagaggtgag gaagatgtta acaaagcgac aaaacgacat
    28741 tttacgtctg atcatccaac actatacgag tagtggcgtg cccgttggtt ccaaaacatt
    28801 gatgtctgag ggagtcaagg ccagctcagc aacgattcgc aatgatatga aagcattaga
    28861 agatgaagga ttgctgttga agactcactc ttcttctggg cggattccat ctgctttagg
    28921 ctatcgttat tacgtggatc atttgttaaa acctgctcgc gtagcagaag atgatttgct
    28981 gctgattcgt cagtctctag gcagagaatt tcatgaaatc aatgagatta ttaagcaatc
    29041 tgctgagata ttatctgagc tgacaagcta tacgactttt tcattgggac ctgaaataaa
    29101 agaccgccga ctgactggct ttcgtatcgt acccttgaat aatcgacaga ttattgctat
    29161 cgtcgtaaca gatatgggga atgtagaaag ccaagtgttc actcttcccg aatatatggg
    29221 aagtcaggaa ctagaaaaaa tggtacagat tgtaaacgac cgtctagtcg gagatcctct
    29281 gatgacggtg taccataaac ttcgtacgga gattccgatg attcttcata aatatttcca
    29341 aacgacagaa gggatgtctc agttatttga caccgttctt ggtcatgttt ttgaagataa
    29401 ggtttatgtc agtggacaaa tgaatctctt ggattatgag ccccgtcaag atcttgatca
    29461 gttcaaatcg atgttttctt ttatgaaaga ctcagacgga cttacacaaa tgatcactcc
    29521 gatggatagc aatattcata tcaagattgg tgctgaatta ggcaatgatt tattgcagaa
    29581 tatgagtatg attcaagcaa gttacgagat agatggtcat ggaagaggta caatagcctt
    29641 gttagggcct actagtatgc cttattctcg gatgttcggt ctagtcgatg tttatcgaag
    29701 agaactagca gctaaacttg ctgattacta ccgttcgctt gacttgtcaa actcgtaatg
    29761 tgaataacga agctatttgc agctgatcgt tctctctttc ttagtaagag caatcaaaca
    29821 gctgactgaa taatgaaagg aagactgtat cgtgaaagaa aatcaagaag aactagataa
    29881 agaaatgaca gatgctcagc cagaaccgga aatcgatgtc gaagctgcag aagattcggg
    29941 tatttcagaa gctgaagcgg aagaattcga aacagcgaaa ttaaaagctg agttagaaga
    30001 aatggaagat aaatacttgc gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa
    30061 aaatgagcgt gagcaattac aaaaataccg ttcacaagat ttagccaaaa aattacttcc
    30121 atcaattgac aacctggaac gtgcattagc cacagaagtg tcagatgatc aaggggcagg
    30181 tttgaaaaaa ggcgtggaaa tggtcttgga aagtttgcgt aacgcactgg aagaagaagg
    30241 aattgaaaaa atccctgcta aaggagaagc ctttgatcct aatcttcatc aagcagtaca
    30301 aactgttcca gcgactgaag atacaccagc cgatacgatt gtagaagtac tacaagaagg
    30361 ctacaaatta catgatcgcg tgttaagacc aaccatggtc attgttgcac aataattgtg
    30421 cggatataaa acttacactt tcaattaaag aaggagacgt taattatgag taaaattatc
    30481 ggtattgact taggtacgac taactctgca gtagcagtat tggaaggaaa cgaagcaaaa
    30541 atcatcgcta acccagaagg aaaccgtaca acaccttctg ttgtatcatt caaaaatgga
    30601 gaaatccaag taggggaagt tgcaaaacgt caagctgtga caaacccaaa tacgatttca
    30661 tcaatcaaac gccatatcgg tgaagcaggt tacaaagtag atgtagaagg taaatcatat
    30721 acaccacaag aaatctcagc aatgatcctt caatatatca aaggattcgc agaagactat
    30781 ttaggtgaaa aagtcgaaaa agcagttatc actgtgccag cttactttaa tgatgcacaa
    30841 cgtcaagcaa caaaagatgc tggtaagatc gctggtctag aagtagaacg tatcgtcaat
    30901 gaaccaactg cagctgctct tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc
    30961 ttagtattcg accttggtgg cggtactttt gacgtatcta tccttgaact aggagacggt
    31021 gtctttgatg tattgtcaac agctggcgac aaccatctag gtggggacga ctttgataac
    31081 aaaatcatcg actacatggt agcagaattc aaaaaagaaa acagcattga cttgtctcaa
    31141 gacaaaatgg ctttacaacg cttgaaagac gctgctgaaa aagcgaaaaa agatttatct
    31201 ggcgtatcaa gcacacaaat cagcttgcca tttatcacag caggagaatc tggaccattg
    31261 catttggaaa tgacattgac acgtgcaaaa tttgatgaat taacagctga cttagtagaa
    31321 cgtacaaaag taccagtacg tcaagcgcta aaagatgcag gtttatcaca atcagaaatc
    31381 gacgaagtta ttttagtcgg tggatctact cgtatccctg cagttgtaga agcagtacgt
    31441 aaagaaacag gaaaagaacc aaacaaatca gttaaccctg atgaagtagt tgctatgggt
    31501 gctgctatcc aaggtggcgt gatcactggt gatgtgaaag atgttgtttt acttgacgtt
    31561 acaccattgt cattaggtat cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc
    31621 aatacaacga ttccaacaag taaatcacaa gtattctcaa ctgcggcaga taatcaacca
    31681 gcagtagaca tccatgtatt acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta
    31741 ggaagattcc aattgacaga tattccagca gctcctcgtg gtattccaca aatcgaagta
    31801 acattcgata ttgataaaaa cgggattgta aatgtatctg cgaaagatct aggtacacaa
    31861 aaagaacaaa aaatcacgat caaatcttct tcaggtctaa cagacgaaga aatcgaacgt
    31921 atggtaaaag atgcagaagc aaatgcagaa gcagataaag ctcgtaaaga ggaagttgac
    31981 ctacgtaatg atgtagatgc attgctattc tctgtggaca aaacattgaa agaattagaa
    32041 ggcaaagtgg acgaagaaga agtgaaaaaa gcagaaacag ctcgcgatga attgaaagcc
    32101 gctgttgaag caaacaacat cgaagaaatg aaaactaaac gcgatgcatt gaacgaaatc
    32161 gttcaaaact tgactgttaa actatacgaa caagcggcac aacaacaagc tcaagaaaac
    32221 ccagaagcag ctcaaggcgg agcagatgat gtcgttgatg ctgattttga agaagtcgat
    32281 ggcgacgata aataatcagt tcactgaatt tcgagagaag gccaaagttg ctactttggc
    32341 tttttctttt gaaaaatagt ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga
    32401 taacaagcga cgtgaaaatg atgagaacca ataggaggaa agcctatggc aacaaaacgt
    32461 gattattatg aagtcttagg gctctcaaaa ggagcatcag aagatgaaat aaagaaagct
    32521 tatcgtaagc tttcgaaaaa ataccatcct gacatcaata aagaacctga tgcggaagaa
    32581 aaattcaaag aagtatcaga agcatatgag attttaagtg acccgcaaaa acgcgctgca
    32641 tatgaccaat acgggcatgc aggcacagat ccaaattacg gtgctggtgg agctggtggc
    32701 ggatttggtg gattcggcgg cttctctggt ggcggcttcg gtggttttga agatatcttc
    32761 gactctttct ttggaggagg cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct
    32821 gatttgcaat atacggtcga cttgtctttt gaagaagcga tttttggtgt agaaaaagaa
    32881 atcaagtata atcgggaaga aatctgtcat acttgtggtg ggaacggtgc aaaaccaggc
    32941 acacaaccaa ctacttgtca taaatgtcat ggtgcaggta cgattaatgt agaacgccaa
    33001 acaccacttg gacgagtgat gagccgtcaa acttgtgatg tttgtcatgg gacagggaaa
    33061 gaaatcaaag aaccttgtcc aacctgtcat ggctcaggac atgagaagaa agcacattca
    33121 gttaaagtga atgtaccagc tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa
    33181 ggagaagctg gagaaaacgg cggaccattt ggtgatttgt atgtggtctt ccgtgtagaa
    33241 gaaagtgata tctttgatcg tgaaggttct gaaatttact atgaattgcc tttaagtttt
    33301 gtacaagcag ctttggggga tgaagtccaa gtaccaactg tccatggaga tgtgaaattg
    33361 aagatccctg ctggaacaca gacaggtacg aacttccgtt tacgtggaaa aggcgctccg
    33421 aagttacgtg gtggtgcaac aggcgaccaa catgtcaaag taaaactgat cacaccgaaa
    33481 aatctaaatg aacaacaaag agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa
    33541 gttgaagaac agcaagaaga cggtttcttc gataagttta aagatgcttt tggcgggaaa
    33601 aagcggaagt agtttttcta gtaaaaacgc taagtcatta ataattaaaa accagacgaa
    33661 caatcatatg ttcgcctggt ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag
    33721 ctttagaata tgataatcgt catagtttct aggggtataa ccagtgagag aatggaactt
    33781 gttcaatcga tattgcaagg tgtttttatg aataaataat tcttctgagc aatttttgat
    33841 gcttccgtta tttttactga agcatgccaa agtcttcttg aacaaatgta cttcttcttc
    33901 tgtcaggctt ttcaaaatac gttttacata taatgttcgt tgatctgacg ggattgctgg
    33961 taaaacgatc ccgaggtcta aatcttcaaa aaagcggatc tcgttcacag attgatggaa
    34021 agctatccat tcgcaaactt gtttggcatg aaggaaagat tgcggtgtct cttgctctgt
    34081 ataacaaagc gacccaattc caaagtttag ctgtaactga tacattttat taaactttgt
    34141 ctggatttta tcaagcaatg atagcaatga ggctctggaa atccgattga ctaggataat
    34201 gatttgttgt ctatgtacag tgagcaaagc ctgtccgtaa taagagaaga ccctttcgag
    34261 caactccaat aaaggattga tccgttcttg acacaattgc tgattgtcag aaagttgtcc
    34321 cacaactaca taatatggag aagaaaaatc aagctcaatt aagtgagggt ggtatcgact
    34381 atgtacttga tatcatcaat gaaccgatgg tactttctta tgcgatggaa caggtagagc
    34441 gattgcttgt ttgtgcagga aaacaagcga tgcggctgat acgattaggt gagaaaataa
    34501 gaagataaaa atacacggtc ccttcatggc gaccgtgcat ttttggtttt tattaaattt
    34561 tatatctaat tttcaattat ccgcataaac actatacatt caagcatttt gaagtataga
    34621 aaaacactca tcttaccacg aatttaccac gattgaatga gccttgatat gaccgtaaaa
    34681 ctactaaggg agataacacg aaactgtgta tctcccgatt tttttataca gttgattact
    34741 tcactccgat ttcaaatttc aatattgctt tcatcatgtc tgtatgtatt tctcctttta
    34801 gttcttcatc aacctgcata atgattttac cactttcaga gtggtaaaaa ggacgcaagc
    34861 ataatttaga aatataaccg tcataaaact tcaaaatctc tttaactgcc atttcatctc
    34921 cgcttgacgc tttgcaaatc aattcaaaag agggatagcc ataatcttta cttcctttcg
    34981 ctcaaaagca tttttaactt ccttagtcct ctagttcttc tactgcaaat agcagaacgt
    35041 gatacatggt acagttctga aatttctctg tcgttcattc cttgaaaata acgtaataaa
    35101 atcgcactac gctgtttttc tgatagtcta tgtaaaactg taccaagtgt ttcatcagat
    35161 acataaattg taaagtgcaa gactttaaat gatacacaat cataaaaaca ggtatcaatc
    35221 gttccacgtt caacttgttt cgtttcggct atttcacaaa acaatacttc acgtttagaa
    35281 tgtcttgcaa gctgtctgtt tcgacttttc actttgttct taatgacaat cagcattaaa
    35341 gaattgaact gtaatctgac gatatgctcg aaagaagatg tcctcataat ctcaccccct
    35401 ttctgtctag gaaaggaagt gagccttgct cgtttatctc ctttcactct tagtcccaat
    35461 gtgaaagggg gatttgttgc attactgata aataaacttt gtaaaaaagt tctgaatagc
    35521 caaaaaagca tataaacaga tttatttctc tgtttacatg cttctgttat tctatctata
    35581 tgatttataa aaccacattg gtggacgtac ttatctattg cagatagacg actttttttg
    35641 acaagaaccc aatgtaagga aatttattgt atatgatgta cttcatggcg acgttgacct
    35701 ccaacaaacc gccatttgga agtaatatac aatattttaa cagcgtaaat agcactacca
    35761 tataacggtt ttttttattg gcgtttagta gtgcttttta ttaaatataa acctataaac
    35821 catataacac gtttttctat acctgttttt aattcagtag gaacaataaa atgtatagag
    35881 gtggtctact atgcgtaaaa aagaagataa atatgatttt agagcctttg gtttagccat
    35941 taaagaagct cgattgaaac gaggtttaac tcgtgaacaa gtgggagcat tgattgaaat
    36001 tgacccacgg tacttaacta atattgaaaa taaagggcaa caccccagca tacaagttct
    36061 ttatgacctt gtatcgttac ttcatgtttc cgttgatgaa tttttcttac ctgctaataa
    36121 cttggtaaaa agcacccgac gattacagat agagaaatac atggatagct ttacagacaa
    36181 agaactatcc ttaatggaat ctttagccag cggtatcaac gaagcaagaa acatcgaaga
    36241 ctaattaaaa gaatccatac ataacggaaa gagccgataa aatgagattg tattaatctc
    36301 attttatcgg ctctgcgtct ttgcgtctgg ctctgtaatc acagttactt tgaactgctt
    36361 tatttcaatt aaattttctt gtctgcattt cggacaatag agggggaatt tttttaattc
    36421 agtatcttcc cttatcttta atcgtgtttt atttccacat acaggacaca atatccactt
    36481 gtagtttata ataactatct cctcctttac actttaattc aaatctttat taaaaaatat
    36541 ttcatcttat ttaacaagaa accatattta tataacaaca taaaatacac taagttattt
    36601 tattgaacat atatcgtact ttatctatcc gactatttgg acgacggggc tggcaaacag
    36661 gttcaccggt agtaacatgg taccctttta actctgttaa acaaacacta cgtccatttg
    36721 taaagaaagt taaatcacta cgatattctt gaatacaccg agcagggatt tctccactaa
    36781 gaatgacctc attatttttc aattgagtgt ctacgatgtt cgcacaatat ttaggagcat
    36841 cgttgtatgc tcgtgaaaga tattcctgtg gcgcataaat tttaaaacta agatatggct
    36901 ctaacaattc tgttccagct ttttttaaga cttgttccaa tacaatagga gcaagcatcc
    36961 gaaaatctgc tggggtacta acagggctat agtataagcc atacttaaaa cagattttac
    37021 agtccgtcac attccaacca tacaatcctt gttcacagcc atagcgtatc ccctccataa
    37081 ctgcattttg aaacgattga tttaagtatc caagagaaac cgagctctca tactgcattc
    37141 cacttcccaa cggaagcggt gatacagata aaccaatgga agcccagaaa ggatttggcg
    37201 gcacttcgat gtgaatggta tattctgcat tttttaacgg tctctccata taaatgactg
    37261 taggctcttt tagttctatc tccacatgat acttttcttg caacagtgca ctaatcactt
    37321 ccatttgtac tttccctaag aaagaaagta taatttcatg tgtcgtagaa tccacgtaat
    37381 atcgtagaag cggatcacta tctgagattt ccaaaagggc atcaagcaac atttctctct
    37441 gttcaggttt actcggttca acagttgttt gtagtagagg gtgcggattt tcaatctttt
    37501 ttctctgtgg caatagtttt gtatctccaa gaacactatt taacttcaaa aactcatttt
    37561 gcaaaataac aatttctcca gaataagctc tatcaatctt acataattca ccatttattg
    37621 aagtatacat ttctgtaact tttatttttt ctttttctga tactctaacc gaatctcgta
    37681 aatgtagtac tccactataa aggcgtatat atgcaagacg ttgtcttttt tttgtatatt
    37741 caattttgaa aacatttccg caaagttcag acggacctcg atgtgttgat gaataaaatt
    37801 tattagtaat aacttctata aggttatcaa tccctatatt actttttgca cttccatgat
    37861 aaagagggaa cagagaacaa ttctgaaatc ttatgctttc ctcttgttcg agttccaatg
    37921 cttctaatga tttaccggac atatatttct ctaaaaggtc atcgtttccc tctattaccg
    37981 tatcccattg ttcagattcg gtaaagttcg tcacacacat attaggatac agttctacct
    38041 tctgtttgat tacaatttcg gcagaaagtt tctctttaat atcctgataa accgttgata
    38101 aatcaattcc attttggtca atcttattga taaaaaagat tgtgggaatc cccattttcc
    38161 taagtgcatg aaataatata cgagtttgtg cttgtacgcc atcttttgca gaaatcagta
    38221 gaattgcccc atctaaaact gataatgaac gatatacttc tgctaagaaa tccatatgtc
    38281 ctggcgtgtc tatgatgttc accttcgtat tttcccactg aaaagaggtt attcctgtct
    38341 gaattgtaat tcctctctga cgttctaaaa gcgtattatc cgtcctcgtt gtacctttgt
    38401 ccacgcttcc taattctgta atcgctccac tgttatataa taagctttct gttaaggtag
    38461 tttttcctgc atcaacatga gctaaaactc caatattaat aattttcatg tgattttcct
    38521 ccattcaaaa acccaaaagg gcataaaaat cccagtgata aatactttta tcactgggat
    38581 ttttatgcat aaccataggc atacaaagca tacagatatt ctccggatac tttagaatca
    38641 catgataaag gtattcttaa actgggtaca aaaaactaag ccctcctaaa aaaggacatc
    38701 caattatttg ttcccactat caaattgaca gtttatttaa gaataccttg ccgcatattt
    38761 attaactcct tttaaataga tacttaaata atagcacgta agagcatatt tgtaaaggaa
    38821 tctccaattt tttatcaaag agagtacgtg attacaaaat agctgtaata atgtaccaat
    38881 atttgttatt ctataatctt ccaattactc ccgttctttt caagtaccaa atcaaattga
    38941 gatacctgcg ttgcctttgc ctgatttacc acaaatcaga ttttatccct ttcttcctct
    39001 tgattgccca caacagaaaa gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc
    39061 tttgcactct tgatcgggct ttttgtttgc tgtatcttct cgcaagagga aaaaagttac
    39121 tcgtcctcat catcaaaatt gttattcctt aatacttctc gtaacagttc catatcttct
    39181 tttgagttcg ggtttatcat tttcacgact tgataaggtg tcttatactt atgtccctct
    39241 atgctttccc caaacatatc catactgtat aaatcatcat agcggtctaa taatttttga
    39301 aacttcaaat gctggataaa ttctttgatt gggtaaaggt cggacgcttc aatgcttttc
    39361 ccgttgatat agtctgtaat atattcccgt aacttttcta actcatattc cagccattct
    39421 tcctcgctgt caaagaagtt atcatagtgt ccatgtttca gttctgcatt tagacgttct
    39481 tcggctctta aaaactgaca gacttccttt tcagattgat tgacatactt ccattctccc
    39541 gacaacaatt cattgggcgt aacgtccagc acttccatta tcttttccag cgtttcatag
    39601 gcaggatagt tcaaccctct ttcaatcttg gaaaggctct gcatattgat accgattttg
    39661 tccgcaagtt cctgttgttt cattccccta aattttctta tggtctgtat attatttcct
    39721 aaatggctta ttttcattgc ataaccctcc atatctcgtt atatcttcgc tattataagt
    39781 ctatcatgct taatatgatt tgtaaaggca aaaaaagata ttgacaaata agcatataag
    39841 aattactatg ttcttgtaat cgaataagca caatagaatg aaatataaaa acattcaaaa
    39901 agaaatacct atctgattaa atccgtagat atgaatatcg gttgaaatgg aacttgttag
    39961 aaaaggcaaa acagaacttg ctgacaaagt atttctgaca cggtggcgga agcctctcag
    40021 gcttaccgcc atgacacctt gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg
    40081 cgagccttgt ctgtgccccc gttatctaac aggggggcac tatttcacaa aagacaagtc
    40141 aaaaaccata caatcccaaa gttgtgagta gtaatgagaa gttttaagaa gatagtgcat
    40201 atcacacttt ttgaatagag gtttacaggt atttcaaggc tattgtggga ttggaggaat
    40261 acaatgaaca atttagaatt tgcacttgaa atgaagaaca aacgtcttgc aagcggtctt
    40321 tcacaaggag aacttgcaag tcttacccat gtatcaagat atagtatcaa ccgctttgag
    40381 aacggaaaag ccaacgccag caaggaaaca caaaatatta tcctgcgttg tctgaattat
    40441 tatatctgcg ataagccttt ttatctgctc gttgattatc tgtctgtccg ttatccaacg
    40501 actgacgcat tggaaattat ccgaaaggtg cttggcatga aagcagatta ttttatccat
    40561 tatgactatg ggtattatgg ctataaagag cattacgcct acggagaaat caaggtcatg
    40621 gcttctgatg acgaacacat gggcgtattc ttggaattaa aaggggcagg ctcacgcaac
    40681 atggaatatg tcttgcaggc acagaacaga gattggtatt catttttaaa ccgctgtctt
    40741 gactgtgacg gtgttatccg acgttttgac ttggcaatca atgatatgtg tggtctgttg
    40801 gatattcccg ttttgtctga aaaatacaga aatggcggtg cggattgtcg ctgtaaaaat
    40861 tatgaaaatg tgcagggtgg aaaactaagc ggaaaaaacc gtaatttagc aagcaccctt
    40921 tatatcggtt caaaggcaag cacaaaatac ttctgcctgt atgaaaaaca aaaggaacag
    40981 gcaaccaaaa agaaacatac ggatattatc aatcgttttg aaattcgtct gcgtggtaca
    41041 aaagcagtac aggcagttga ggaattgtta ttgacatata accctcatgg gctggtgttt
    41101 taccttatta ctgattttgt gcaatttccc gactatccgc tgtgggagat attcatttcc
    41161 catgacagtt taccttttga aatgaacccc gtacctgtca atatggaacg cactctgcaa
    41221 tggctggaaa gacaggtcat gccgtcaatc gtgatgatag aggaaatcga caggctgaca
    41281 ggctcaaact acatgaaaat gattgatgaa tgtacccacc tctctgaaaa gcaggaaatg
    41341 cttgtggaac agatgtgtac ggacatagca gacgtaatcg aaagtgaggg ggtgttttat
    41401 gagtaatgca caggatattc ccgtatggga aaaatacacc cttaccattg aagaagcgtc
    41461 aaagtatttc cgtatcggag aaaacaagtt aagacgattg gcagaggaaa acaaggacgc
    41521 tggctggctc attatgaatg gcaaccgcat acagattaaa cgccgacagt ttgaacaggt
    41581 cattgataaa ttggacgcta tctaatgcaa atgagccttg tatgtgttat gatgaacaca
    41641 agtcatatca aggctctttc caacaaggaa aggagcagac accatgaaag aaaaaagacg
    41701 ggataacaaa ggtcgtatcc tgcatactgg agagagccaa cgaacagaca gaaaatactt
    41761 atataaatat gtagacgcat ttggaaacac aaaatatgtg tatgcttgga gattgacacc
    41821 cacagacccg acaccaaagg gaaaacggga aaaaccctca cttcgagaac tggaacagca
    41881 gataagacgg gatattgagg acggtatcga cagcactggc aagaaaatga cgctttgcca
    41941 actttatgcc aaacagaacg cacagagggc aaatgtgaag aaaagcacac agaaacaacg
    42001 ggaacaactc atgcggttat tgaaagagga caagttaggt gctaggagca ttgatacaat
    42061 caaaccctct gacgctaagg aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa
    42121 taccattaac aaccataaac gctcgttaaa agcgtcattc tacatagcca ttcaagatga
    42181 ttatgtgagg aaaaatccct ttgatttcaa gttgagtgaa gtcctagaaa atgataccaa
    42241 aaagaaagtc gcattgacag aggaacagga acaagcctta ctctcattca tcaagacgga
    42301 caatgtgtat cacaagcatt atgatgatgt gctgatactg ttaaagacag gacttcgtat
    42361 ttcggaactg tgcggactga cagtagccga cattgatttc aagaatgaag ttgtgattat
    42421 cgaccaccag ttactaaaga gcaaggaaca gggctattat attgaaacgc ctaaaacaaa
    42481 aagcggaata aggcaagtac cattaagcaa agaaacgata caggcatttc aacaggttat
    42541 gaaaaaacgt ccaaaggcag aaccatttgt gatagacgga cggggcaact tcctatttgt
    42601 caatcataag ggcaagccca aagttgcgat tgattataac gccttattta tccgtatggt
    42661 aaagaaatac aacaagcacc acaaggacaa tccattgcca catatcacac cgcatacgct
    42721 acgccataca ttctgcacaa gactggcaag caagaacatg aacccaaaag atttacagta
    42781 tatcatgggg cattcaaata tcagtatcac aatgaactgg tatgctcatg cgtccataga
    42841 taccgcaaaa tcagaggttc agcgtctaat tgcatagaag tatttaccac gattttaacc
    42901 acgcttgata gtgaaaatat aagaagatag acctagatat gtgaggttta ccacaaaagc
    42961 aaaatgcccg tagagcctat aaaataaggc tttgcggaca tttaagaaga tataaaaaga
    43021 tagtcaaaaa gacatatata atttatttta aatatttaat actttattca agaaatcttt
    43081 tgtccgttca ttttgcggat tttcaaagat ttgttgaggc gttccatctt ctaaaaagtt
    43141 tccgtcgtca atgaacatga cgcgattggc cacttcttta gcgaacccca tttcgtgcgt
    43201 aacgataacc atcgtcatac cttgttctgc taactttttc atgacgttca agacatcacc
    43261 gaccatttca ggatcgagtg cagaagtagg ctcatcaaaa agcatgatgt cagggttcat
    43321 cgctagagca cgagcaatag ccacacgctg tttttgtcca ccagaaagca tttcaggata
    43381 catatctttt ttatcttcta gaccaactgt ctgcagtaat ttgattgctt ttttctctgc
    43441 ttcttctcta gattctttct tcaggtccat cggtgctaaa atgatgtttt ccaaaataga
    43501 aagatgaggg aataggttaa agtgttggaa taccattccg atatgttgtc tcaccaagtt
    43561 gatatctgtg cttttatcca tcaagtgggc gccgtcaata ataatttcac cgtcagaagg
    43621 ttcttctaaa cgattcaagc aacgcaagaa tgtacttttt cctgaacctg aagggccaat
    43681 gatacagacg acatctcctt cgttgatgga tacattgatg tcgtttaata ctgtgttatc
    43741 gccgtatttt ttgactaaat gctcaactaa gattttttct gccatcttac ttcaccttct
    43801 tttctaaaac ttttgctaat tttgtcaatg ctgtgatcaa aatcaagtac atgatcgcaa
    43861 tgatcaagta aacatacgta ctttgtgttg tacgtgcaac aatgatctta ccagtttgca
    43921 gcaattcgac tacaccgatt gcggaaatga tcgttgtgtc ttttaatgag ataacgaact
    43981 gattgacgaa tgatgggatc atgatgcgta tcgcttgcgg caaaatgatt ttttgcattg
    44041 tccgattata agaaagacct aaacttctgg aagcttccat ttgtccgaca ggaacggcgt
    44101 tgatcccgcc tcgaacaatc tctgcgatat aagcacttgc gttaagagtc aaggtgataa
    44161 caccagcaag gaagtcagga atcgtaaatc caacgatacc aggcaaccca aagaaaataa
    44221 agaacgcaag aaccatcatt ggaattcctc ggatgatatc gacataaata ctagcaattg
    44281 tgcgaagagc gcgtattggt gctacactga ataatccaaa aatcacaccg attactatag
    44341 ccaaagcaaa ggagatcaaa gttaggagaa ttgttctcca aagtcctttc aacaatgctt
    44401 gatagttgtt ttggataagt ccaagaaatg ttgattcatc agcagtggaa gactcactgg
    44461 aagtttctga accatccgct acatattctt taataatctt atcgtattcg cctgttcttt
    44521 ttaattcttt caatccttca ttgaacatat taagaagctc aggattttgt ccttttttaa
    44581 cggcaaatgc atattttccg ccagattctt ttttgatcgg tgtttccaat tcttgtcctt
    44641 gtttgatcgc ataaccgatt accggataat catccatcat tgcatcgatt gcgccaactt
    44701 ttaacccgtc gtacaattga tcagcagcat caaaatattt gatagaatag ccatattcag
    44761 attcatggtc gcttaaaaat tcagcacttt cggtaccaac ttttgctccg actgtttttc
    44821 ctttcaaatc ttcataagac ttgatcgaag tatctccttt tttgacagct aattggatcc
    44881 cgctatcaaa gtaagaatca gaaaaatcat agcttttctt tcgttcatct gttacagtca
    44941 ttccggcaat cattccgtct gcttgtcctg attccaaagc ttgcaaagca gcagagaaac
    45001 cacggtagtc cattcggatg ttaaatcctt gcagtttggc gatgttttcc atcaatttta
    45061 catcgatgcc ttcgtactcg ccctttgcgt tttgaaattc aaatggagca aaagcagagt
    45121 cactggcaat tacgtatacg tcttttttgg gctctatttt ttgcattgtc tcgcttgtgc
    45181 ttgactctgc cgttgtagtg ttgtcagctt gtgcaacatg accaaacgca agtgtcacaa
    45241 gtcctgtcat taagaaaaca aatgagtgaa ataaatgctt tcttttcata atcaacctcc
    45301 taaatttcca atattttatt attataggcg aaaagtgctg aatcagacaa gactgtaagc
    45361 tcgaaaaaat gaaaaaaatg cgagtttcta acattttatg ttataaaaca taacatgtat
    45421 aataagataa aaaagtggcg aaagtatgtt cggtgtggta taatttttta gtaaggaatg
    45481 cctttgcttt ccttttcttt ttattggtcg taaaatgaaa aggaatgaat gaaaaggagg
    45541 acgaagaatg attgagagag atacctcccg acactttgag cttgtctcga aataccaacc
    45601 ggctggtgat cagcctgaag cgattaacca attagtggat ggcgtagtcg gcgggaaaaa
    45661 agcacaaatc ttattaggtg cgactggaac agggaaaaca tatacgattt ccaatcttat
    45721 tgagaaagtg aataaaccaa cactgattat tgcccataac aaaacattgg ctggtcagct
    45781 gtacggggaa ttcaaggaat ttttccctaa caatgctgtt gaatattttg tttcttatta
    45841 tgattattat cagcctgaag cttatgtgcc ttcaagcgat acgtatatcg aaaaagattc
    45901 aagtgtaaat gatgagatcg acaaattacg acattcggct actagttcat tattagaaag
    45961 aaatgatgtg atcgtcattg catccgtttc atgtattttt ggattaggct cgccttttga
    46021 gtaccaaaaa caagtagttt cgattcgaca gggagcagaa ctagatcgca atcagttgat
    46081 tcgtgactta gtcagtatcc aattcgaaag aaatgatata gattttcagc gcggtcgttt
    46141 tcgagtgcgc ggtgatgtcg tggagatttt tccggcttca agagatgaac gtgccttacg
    46201 tgtggagttt tttggagatg aggtcgaacg tatccgagaa gtcaatgcac tgacaggtga
    46261 agttttagga gaaacggaac acgtagcaat cttcccggcg actcactttg tgacgaatga
    46321 tgaacatatg gagcatgccg tagcgaatat caaagaggaa ttagaacagc gtttaactgt
    46381 cttgcgaaat gaaaacaaac ttctggaagc tcaacgttta gaacagcgaa ccaattacga
    46441 tattgaaatg atgttagaaa tgggctacac gtcgggaatt gaaaattatt ctcggcatat
    46501 ggacggacgt aaagaaggtg aaccgcctta tacgctattg gatttctttc ctgaagattt
    46561 tttgatcgtg gcggacgaat cgcacgtaac catgcctcaa atcagaggaa tgtataatgg
    46621 tgaccgagcg agaaaacaga tgctagtaga ttacggtttc cgtttgccat ctgctttaga
    46681 caatcggcca cttcggttag aagagtttga aaaacatgtc aatcagatca tttatgtctc
    46741 tgcgacgcca ggaccatatg aacatgaaca aacagatacc gttatccaac aaatcatccg
    46801 tccgacaggg ctgcttgacc cagtgatcga agttcgtcca atcatgggac aaatcgatga
    46861 tctagtaggc gaaataaatg aacgagtcga aaaagaccag cgggtgtttg taacgacatt
    46921 gacgaaaaaa atggcagaag acctcacaga ttatttcaaa gaattaggga tcaaagtcaa
    46981 atatttgcat agtgatatca aaacgctgga acgaacagaa atcattcgag atcttcgatt
    47041 aggtgaattt gatgtgttag ttggaatcaa tttgcttcgt gaaggattag acgtacctga
    47101 agtttccctt gttgctatct tggatgcgga taaagaaggc tttttacgta gtgaacgttc
    47161 cttagtccaa acgattggac gagctgctcg gaatgaagaa ggtaaagtaa tcatgtacgc
    47221 agacaaagta acagattcta tgcgtctagc gatggatgaa acgtcaagac gtcgtacgat
    47281 tcagcagaag tacaatgaag aacatgggat cgttccaaaa acgatcatta aagaaatccg
    47341 agatttgatt tctattacga aagaatcgga agatgataca aaagaagcgg tacaagtctc
    47401 gtacgaagaa atgacgaagg aagaaaaaga cacgttattg atgaaattag aaaaagaaat
    47461 gaaagatgcg gcaaaagctt tggattttga aacagccgcg aatgtgcgag atatgatttt
    47521 agaattaaaa gcttcaaagt aggaggacaa catggctaac gataaaatta cgattcatgg
    47581 tgcacgtgcc cataacttga aaaatataga tgtaacgatt ccacgagata aattcgtggt
    47641 cgttacaggt ttgtccggtt ctggaaaaag ttccctagca tttgatacgc tgtacgctga
    47701 agggcagcgg agatacgtag agagcctttc tgcttatgct cgtcagtttt taggacaaat
    47761 ggataagccg gacgtagata gcatcgacgg attgagtccg gcgatttcta ttgatcagaa
    47821 aacaacaagt aaaaatcctc ggtcgacagt aggtactgtg acggaaatca atgactattt
    47881 acgcttgttg tttgcacgtg taggacatcc gatttgtccc aacgatcata tcgagattac
    47941 cagtcaatca gtagatcaaa tggcagatca agtaatggct ttgcctgagc gcacaaagat
    48001 ccaagtgttg gcaccgatcg tggtaaagaa aaaaggacaa cataaaaaaa tattcgaacg
    48061 aatccaaaaa gagggatacg tccgcgttcg agtcgatggt gagatctatg atttaagtga
    48121 agtgcctgag ctggagaaaa acaaaaaaca tgatatcgcg atcgtcatcg accggatcat
    48181 tgtgaaagaa gggattcgtt ctcgcctatt tgattcttta gaggcggccc tgcgattagc
    48241 agacggctat gctctggttg acgtgatcgg tgaagaagaa atgttgttta gcgaacacta
    48301 tgcctgtcct tattgcggat ttactgttgg cgaactagaa ccaagactgt tctcattcaa
    48361 tgcgcctttt ggagcttgtc cagactgtga tggtttaggt gtcaaattag aagtcgatca
    48421 agatttagtg attccagata gaacgaagac acttagtcaa ggtgctatcg ttccatggaa
    48481 tccaatcagc tctcagtatt atcctcaaat gttagcgcag gcttgtgaaa gtttcggtat
    48541 cgatttagat acaccattcg aagaattacc ggaagagcat caggagatca tcttgaatgg
    48601 gacagatgat cttttccatt tccattatga aaatgatttt ggtggcgttc gcgatgtaga
    48661 aacgactttc gaaggggtat tgaagaatat cgagcgtcgt tatcatgata cgaacagtga
    48721 tttcacgcgt gagcaaatgc gtctttatat gacagaatta acttgtcaga catgtaaagg
    48781 ttatcgattg aatccccaag ctctatctgt tcaaatcaat ggaacgaata tcgggcaaac
    48841 aaatgagttg tctattcaaa aagcagtaga tttcttctca aacttaacgt tatctgaaca
    48901 agagaaagta attgccaaac cgattttaaa agaagtaaat gacagacttt cattcttgga
    48961 aaatgtggga ctagactatc taactttgag tcgcgcttcc ggaacattat ctgggggaga
    49021 agcgcagcga atcagattgg ctacacaaat tggatcgaat ctttctggag ttttgtatat
    49081 tttagatgaa ccatcgatcg gattgcatca acgcgacaac gatcgactga ttggctcatt
    49141 aaagaaaatg cgtgatcttg gcaacaccct cgttgtagtc gaacatgatg aagatacgat
    49201 gtgggcagca gattatttga ttgacgtcgg acctggggcc ggagaacaag gcggacaaat
    49261 cgtggctgca ggcactcctg aagaagtagc taatgatgaa aattctttga cagggaaata
    49321 tctttcaggg aaaaaatcaa tccctgtacc aaaagaacgc cgtaaaggta atgggaaagc
    49381 aatcaagatc actggtgctt cagaaaacaa tctgaaaaat attagtgttg aatttccatt
    49441 aggagaattt gtagcagtca caggtgtgtc tggttctgga aagagtacat tggtcaatag
    49501 tatcttaaag aaatcgttag cgcaaaaatt aaataagaat tctgctaagc caggtaaatt
    49561 caagacaatt tccggctacg aaagtatcga aaagatcatc gatatcgatc aaagcccaat
    49621 cggccggacg ccgagaagta atccagcgac ttatacaagt gtatttgatg atatccgtgg
    49681 gttatttgct caaacgaacg aggcaaaaat gcggggttat aagaaagggc gttttagttt
    49741 caacgtaaaa ggcggtcgtt gtgaagcttg tcgcggggat ggaattatta agatcgaaat
    49801 gcactttttg cctgatgtct atgttccttg tgaagtatgt catggtaaac gatataattc
    49861 tgaaacatta gaagtgcatt acaaaggaaa aagcattgct gatattttgg aaatgacagt
    49921 agaggatgct gtagaattct tcaagcacat tccaaagatt catcgcaaac tgcaaacgat
    49981 tgttgatgtt ggcttaggtt atgtgacgat ggggcaacca gcaacgacat tgtccggtgg
    50041 tgaggcacaa cggatgaaac ttgccagtga attgcacaaa atctctaatg gaaagaattt
    50101 ctatatacta gatgaaccaa cgacaggact tcatagcgat gacatcgccc gcttgttgca
    50161 tgtattacaa agattagtag atgctggtaa cacagtttta gtgattgaac acaatctaga
    50221 tgtaatcaaa acagcagatt atatcattga tttaggacca gaaggtggag aaggtggagg
    50281 aacgatcctt gcgactggaa caccagaaga aatcattaac gtaaaagaaa gttatacagg
    50341 tcactatttg aaaaaaataa tggtataaag aggatagata gctgagatag ttaaaaaaac
    50401 ataaaaaaat gaatttttgc ttgttttatt aatatcagtg ttctagaatc attacatggt
    50461 agttctttct acactgattg gtatagataa aaacttccta taagttcaat ccgttcaaaa
    50521 aagaattgag caatcggagg actttccttt gattgctttt ttctttatgg tatgatattc
    50581 cttgtgtagt tggaaaagaa aaacaagaga ttatgagtaa gtgagagata atgatgaaca
    50641 gtattgaaaa tgaagaattt atccctatat tattaggaag cgacatgaac gtttatggta
    50701 tggcacgttc ttttaatgaa gcatacggca aaatctgcca agcatatgct tcggatcagt
    50761 tagcccctac ccgctacagt aaaattgtca atgtagaagt tattccagga tttgataaag
    50821 accctgtatt tatcgagacg atgcttcgtt tggcaaaaga aagatattct gataagagta
    50881 aaaaatatct tttgattgct tgtggtgatg gatatgcaga attgatctcc caacataaac
    50941 aagagctgtc agaatatttt atctgtccat atattgatta ttctttattt gaaaggctga
    51001 tcaataaagt aagcttttat gaagtttgtg aagaatatga tcttccgtat cctaaaacat
    51061 tgatcgtcag agaagaaatg ttagtaaatg gacatttgga acaggaattg ccttttgaat
    51121 tcccagttgc tttgaaacct gcaaacagtg tggagtatct ttctgtccaa tttgaaggaa
    51181 gaaaaaaagc ttttatttta gagactagag aagagttcga tttaatttta ggccggatat
    51241 acgaagccgg ttataaaagt gaaatgattg tacaagattt tatcccgggt gatgatagta
    51301 atatgcgtgt gctaaatgcg tatgtagatg aagatcatca agttcggatg atgtgccttg
    51361 gtcatccact attagaagat cctacaccag catcgattgg taattacgtt gtgatcatgc
    51421 cagattataa cgaaaaaatc tatcaaacca ttaaggcatt tttggaaaaa atcgaatata
    51481 caggctttgc caatttcgat atgaagtatg atcctagaga tggagaatat aaattatttg
    51541 aaatcaattt gagacaaggt cgtagcagtt tctttgtgac attgaacggc ttgaatctgg
    51601 ctcgttttgt cacagaagat cgagtgttca acaaaccctt tgttgaaacg acctatggaa
    51661 cgaatcaatc agataaagca agattatgga tgggtgtgcc caaaaaaatc tttttagaat
    51721 atgctcggga aaatgaagac aaaaaactag ctgagcaaat gatcaaagaa aatcgttacg
    51781 gtactactgt attttatgaa aaagaccgat caatcaagcg ctggttgttg atgaaatata
    51841 tgttccataa ctatattcca agattcaaaa agtattttca tgtgaaagaa ggctagttca
    51901 tggagaattt tttcagtatt ttaggcggaa tgggcacgat ggcgacagaa agttttgttc
    51961 gcttgatcaa ccaccgaacg aaagctacaa aagatcaaga atatttgaat tatgtcttat
    52021 tcaatcatgc aacagttcct gatcgtacag cttatatttt agatcgatca gaagaaaatc
    52081 caatgccgtt tctgctggat gatattgaga aacaaaattt attgcgacca aacttcattg
    52141 tgttgacttg taacaccgca cactactttt ttgaagagtt acaagcggca acggatattc
    52201 ctatcttgca tatgccaaga gaagcagcaa atgaacttgt ccgtcagcat acgacaggga
    52261 gagtagcgat tctaggaaca gaaggaagta tgaaagctgg aatctatgaa cgcgaagtca
    52321 aaaatcttgg tttcgaaaca gtgattcctg atactgcgct tcaagaaaaa atcaattacc
    52381 tgatctatca tgagatcaag gaatcggatt atttgaatca agagctctat tacgaaatct
    52441 tagaggaagc ggtagagcgc ctaaactgtg aaaaagtcat cctaggttgc acagaattat
    52501 ctttgatgca tgaatttgct gaagataatc attacccagt gatcgatgct caatctattt
    52561 tagcggatcg aacgatcgaa cgtgcattag ctgaaagaag cgaggcattg gatacagcat
    52621 cggaaaagta aaaaaattgg atttggggca aatatcctaa aagatatttg gtccaaatct
    52681 ttttgtaaaa gaaaaagaaa actggtaatg atacgcccgg gatgatataa ttttattatg
    52741 cacaatgtga gaggggttcg taaatatgac agataatttg caattagtca tcataacagg
    52801 tatgagtggt gcagggaaaa cagtagctat ccaaagtttt gaagatatgg gctatttttg
    52861 tatcgataat atgccgcctc gattgattcc aaaattctgg gaattgatca aagaatcagg
    52921 taaagtcaca aaaatggctt taggagtgga tttgcgttct cgttccttct ttgaagaaat
    52981 ccaaaacatg ctgatcgaaa tcgaaaatac gaacttcatc gacacttcta tcttattttt
    53041 agatgcttca gacgaagaac tagtggctcg ctacaaagaa acaagacgag cacatccaat
    53101 ggcgatggac ggtttgatca ctgaagggat acgtaaagaa cgggcgatac tagacgactt
    53161 gaaagcccaa gcttccgtga tcattgatac gacaacattg acaccacgac aattacgtga
    53221 aaaaatcaat gagtcattca aatcaaaggg agaccaagga tttcggatcg aatttgtatc
    53281 atttggtttt aaatacgggt tgccgatcga ttcggatatt gtgatggatg tgcgtttttt
    53341 gcccaaccct cattatatcc cggaactaag accattgacc ggaaaagatc agccagtcta
    53401 tgattatgtg atgtcctttg atgaaacgca atcattctac cataaatttt tggatcttct
    53461 ggaaactgta ttgcctggat acattaaaga aggaaaaagc agcttgacag ttgcaatcgg
    53521 ctgtacaggc ggtcaacacc gttctgtggc attgactgaa cgtttggcaa atgctttagg
    53581 gaaaaactat aaagtcaatg tgacacatcg ggacaaggat aaaaggaaag agacggtgaa
    53641 ccggtcgtga aaatgaaaac ctaccggata agaaaaccaa agatcgtagt ggtcggcggt
    53701 ggcactggtc tgccagttat cctgaaaagt ttacgaaatc aaggagcaga tatcacagca
    53761 gtagtgactg ttgcagatga tggtggaagc agcggtgcaa tcagagaatc gatcgctatg
    53821 gctcctcctg gtgacttgag aaatgtctta gtggcgttat cggatatgcc acaattctat
    53881 gaagatattt tccaataccg tttcaaaaag gaagatcagt ttttagccaa tcatacgatc
    53941 ggtaatctga tcattgccgc agtttccgag atgcggggaa gcacttatga ggcgattcaa
    54001 ttattagcaa aaatgatgca tgtagatgga catgtttatc cttcttcaga aactcctttg
    54061 accttgcatg ctgtttttaa agacggaacg actgcagtag gagaatctaa aatcgcgatc
    54121 aaccgtaaaa ccattgaccg tgtgtatgtc tgcaacacaa atgataaaag tgaagccaaa
    54181 gcggctcgaa aagtcgtttc agcaattatg gacgccgaca tggtcgtttt gggacccgga
    54241 agtttattca ccagtatcct tccaaattta gtgataccag aaatcggcga agcaatgctc
    54301 aagacaactg ctgaaaccgt ctatatctgt aatatcatga cccaaaaagg ggaaacggaa
    54361 catttcacag atgccgatca tatacgtgtt ttacacaagc atttgaatca gccttttatc
    54421 gatacagtat tggtcaatac ggaaaaagtg cctgaagatt atatggaccc agagatctat
    54481 gatgagtatc tagtacaagt cgcacacgac ttcaacggat tgagaaatga aggatgtcga
    54541 gtgatttcca ccgacttttt agaattgaaa gatggtggtg tcttccacga tggagacaaa
    54601 gtagtagaag aattgttccg tttagttttc ggttcaaaat attaagcaat ttattgttgg
    54661 agaaaggagg agaataggat gtcatttgct gcagatgtga agaaagaact aactggtttg
    54721 gaagttcatc gagaacatgc gaaagcggaa ttagctgctt tgctgcgaat gaacggctct
    54781 ttaggtattg tgaaccagca gtttattttg aacgtccaaa cagaaaatgc tgcaattgcc
    54841 agacgtatct attctctttt gaaagatcat tatcaggtac gaagtgagct actcgttcgt
    54901 aaaaaaatga aattaaaaaa gaacaatgtc tatatcgtcc ggttgaagca aggcacaaaa
    54961 gatgtgttga tggatcttga tatcatggat ggtatgatgt tcaatggaca cgtttcggac
    55021 gagattatgg gaaattcgca aaaaatgcgt tcttacttac gtggtgcttt tatggcctca
    55081 ggttcagtca ataatccgga aacgagtcgt tatcatttgg aaatattttc tatttatgaa
    55141 cagcataacc aagacatttg cgatatgtta aattattacg gtctacatgc tcgaacgctt
    55201 gaacggcgca atggctatat ttcgtattta aaaggagcag aaaaaattgc tgatttctta
    55261 acattgattg gtgcaaccaa ctcaatgctc cgatttgaag atgttcgaat cgtacgggat
    55321 atgagaaatt cggtcaatcg tctagtcaat tgtgagacag caaatctgaa caagacgatt
    55381 gatgcggctt ctaaacaaat cgaaaacatc gaattgatcg aagcaaaggt cggtctacat
    55441 gcccttcctg aaaaacttca ggaaatcgca gaacttcggc ttcaacatcc agaggtcagc
    55501 ttgaaagaat taggggaaat gattccctca ggtgcaatat caaaatcggg aattaatcac
    55561 cgaattcgca aaataaatga atttgcggaa aaactgcgtg aagaagcagt ttaatccaaa
    55621 gaaaaaactg atacatgaag caggagatgc gaatgtatca gtttttttta aaagctatac
    55681 ttgcttttct tccgtcaaac tataattcca actttactgg agtaacataa tgttgaaagg
    55741 cttctattgt tttttaatac aatcgtataa aaagcgggga gacaactgaa tgaagatttt
    55801 agttttatac ataaactttt gcttaatata gaagtcctga tatagatagg atcaagtatt
    55861 tatctataac tggagattaa ttaaaaactg gaaggaactg ctcgatcaac cttgagaact
    55921 gtttttctta cttctggtca agattctcgt ggttggtgga taaggatggt tgataagttc
    55981 attggattag ctagtcgaaa acaaccaggt gtaattaatg tagaagtttt aacaagggga
    56041 tggagtgatt tattcagtga taatcataag ttcataaata gtaattggga tgtatggttg
    56101 gtatacgtta tacctgaact tgaactatca agatttggtt tagaatttga gaatcgatgt
    56161 ttataaaggg agggcaaaaa atgtgctttt ttacaacttt tactatcatt atgattgcag
    56221 tactttttat ttttctagat atagcaaagc gaaatacagc atttttatta tatcgggttt
    56281 tacttcgagc aggtctcata acatttatat cgatcgttgg attttttcta ttcacagtta
    56341 ttgtatttat ttggagaact acagcaccac cattgccaga gattacatat ggagaatttc
    56401 cgtttagtct tgaatatgaa atagatgagg aaagaatttt gattgaagat acggtgattg
    56461 cagaatttat aggaagtttt agagggaatg tgacaactca acctagacga agttggagta
    56521 taaaactttt aaataatagt ggagagttga aagcatttca ttatgaattc ttaataatag
    56581 aaagtgaaga tgtaacaatc actttaagta gtggctctgc tggatatttt atgaatgatg
    56641 gtggaagcaa gccacagctc acagtccgtg atcgtattgc aggttttatg catttttctc
    56701 actatgactt agaagaagct cgtaaaatac ttgcagaata tgggatcaca ttaatcaatt
    56761 gggaatatgc gccaccgata agaaattttt tcaatgatta ttattagtct tataaaaaac
    56821 agaatgtaaa atttatgtgc gcgagtattt agttttccat tttttgtttg atagatatat
    56881 agcattgttg aagcatggga tagtggtgcc aattttatca atgaggttgg tcaggcagtt
    56941 aacataattg gtgatgctat aaatccattt aattggaatt ggaggaatta ggtgtggaaa
    57001 cagtgagatt atggatattc atgaaaaatt ttcgttcggc tttgagatat gttattctgg
    57061 aatcaaaatc aggttattat ctgattgaca cagatcacta tactttttgg ggaggctacc
    57121 tttttccgat ttttaattgg ataatccccc ataaggttat gaaaattaac ttgcctgcag
    57181 aagaaataaa tagattaata ctcaacaaag atttacaaag gaaaagagag aaagaacatt
    57241 ctaatgttgc ttggataggt ccagcatttg cagtagtgac ttcttcaatt gtcggtccta
    57301 tgctagagag tatttatagt gatttttcta ttggaatacg ttatttattt gttactttaa
    57361 gtatagctgt tcttttgtgc ataaagggtt atgttagcaa gaagaataaa gatattttaa
    57421 cactaatcgg tgtaaacaat ttatctagta ctaaaattat actgtttcct gaatctataa
    57481 aagaaggatc aaggttgttg ataattttta taatgcttat actgttttct gttttatttg
    57541 gggtgatgtt tataga
//
LOCUS       DACECR010000006        52210 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.006, whole genome shotgun sequence.
ACCESSION   DACECR010000006 DACECR010000000
VERSION     DACECR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.006"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            290..1135
                     /locus_tag="IWO17_000317"
     CDS             290..1135
                     /locus_tag="IWO17_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0289217.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="IWO17_000318"
     CDS             1416..2636
                     /locus_tag="IWO17_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ0289218.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="IWO17_000319"
     CDS             2633..5353
                     /locus_tag="IWO17_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0289219.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="IWO17_000320"
     CDS             5337..6281
                     /locus_tag="IWO17_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0289220.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="IWO17_000321"
     CDS             complement(6350..7360)
                     /locus_tag="IWO17_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0289221.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="IWO17_000322"
     CDS             complement(7540..7884)
                     /locus_tag="IWO17_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0289222.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="IWO17_000323"
     CDS             complement(7877..8311)
                     /locus_tag="IWO17_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ0289223.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="IWO17_000324"
     CDS             8449..9186
                     /locus_tag="IWO17_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0289224.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="IWO17_000325"
     CDS             9179..10390
                     /locus_tag="IWO17_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0289225.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10597..11376
                     /locus_tag="IWO17_000326"
     CDS             10597..11376
                     /locus_tag="IWO17_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ0289226.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="IWO17_000327"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="IWO17_000327"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ0289227.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IWO17_000328"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IWO17_000328"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ0289228.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="cpaF"
                     /locus_tag="IWO17_000329"
     CDS             14092..15021
                     /gene="cpaF"
                     /locus_tag="IWO17_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0289229.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="IWO17_000330"
     CDS             14978..16027
                     /locus_tag="IWO17_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ0289230.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="IWO17_000331"
     CDS             16024..16302
                     /locus_tag="IWO17_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ0289231.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="IWO17_000332"
     CDS             16277..16747
                     /locus_tag="IWO17_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ0289232.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="IWO17_000333"
     CDS             16764..17066
                     /locus_tag="IWO17_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289233.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="IWO17_000334"
     CDS             17083..17454
                     /locus_tag="IWO17_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ0289234.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="IWO17_000335"
     CDS             17426..17785
                     /locus_tag="IWO17_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289235.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="IWO17_000336"
     CDS             17957..18964
                     /locus_tag="IWO17_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0289236.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="IWO17_000337"
     CDS             18989..20173
                     /locus_tag="IWO17_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ0289237.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="IWO17_000338"
     CDS             complement(20610..22193)
                     /locus_tag="IWO17_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0289238.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="IWO17_000339"
     CDS             complement(22771..22914)
                     /locus_tag="IWO17_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ0289239.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23136..23615)
                     /locus_tag="IWO17_000340"
     CDS             complement(23136..23615)
                     /locus_tag="IWO17_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0289240.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23688..23972)
                     /locus_tag="IWO17_000341"
     CDS             complement(23688..23972)
                     /locus_tag="IWO17_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289241.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24050..24244)
                     /gene="ssrS"
                     /locus_tag="IWO17_000342"
     ncRNA           complement(24050..24244)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWO17_000342"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24316..25602)
                     /locus_tag="IWO17_000343"
     CDS             complement(24316..25602)
                     /locus_tag="IWO17_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ0289242.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25740..26411
                     /locus_tag="IWO17_000344"
     CDS             25740..26411
                     /locus_tag="IWO17_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ0289243.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26377..26865
                     /locus_tag="IWO17_000345"
     CDS             26377..26865
                     /locus_tag="IWO17_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ0289244.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26878..27825
                     /gene="prmA"
                     /locus_tag="IWO17_000346"
     CDS             26878..27825
                     /gene="prmA"
                     /locus_tag="IWO17_000346"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ0289245.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27826..28590
                     /locus_tag="IWO17_000347"
     CDS             27826..28590
                     /locus_tag="IWO17_000347"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ0289246.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28823..31036
                     /locus_tag="IWO17_000348"
     CDS             28823..31036
                     /locus_tag="IWO17_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ0289247.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31047..31493
                     /locus_tag="IWO17_000349"
     CDS             31047..31493
                     /locus_tag="IWO17_000349"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ0289248.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31664..32923
                     /locus_tag="IWO17_000350"
     CDS             31664..32923
                     /locus_tag="IWO17_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ0289249.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33012..33959)
                     /locus_tag="IWO17_000351"
     CDS             complement(33012..33959)
                     /locus_tag="IWO17_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0289250.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34353..35645
                     /locus_tag="IWO17_000352"
     CDS             34353..35645
                     /locus_tag="IWO17_000352"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ0289251.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35660..37429
                     /gene="aspS"
                     /locus_tag="IWO17_000353"
     CDS             35660..37429
                     /gene="aspS"
                     /locus_tag="IWO17_000353"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ0289252.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37538..38413
                     /locus_tag="IWO17_000354"
     CDS             37538..38413
                     /locus_tag="IWO17_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0289253.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38679..40562
                     /locus_tag="IWO17_000355"
     CDS             38679..40562
                     /locus_tag="IWO17_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0289254.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40637..40921)
                     /locus_tag="IWO17_000356"
     CDS             complement(40637..40921)
                     /locus_tag="IWO17_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0289255.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40911..41645)
                     /locus_tag="IWO17_000357"
     CDS             complement(40911..41645)
                     /locus_tag="IWO17_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ0289256.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41774..42397
                     /locus_tag="IWO17_000358"
     CDS             41774..42397
                     /locus_tag="IWO17_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ0289257.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42589..43710
                     /locus_tag="IWO17_000359"
     CDS             42589..43710
                     /locus_tag="IWO17_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ0289258.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43717..46845
                     /locus_tag="IWO17_000360"
     CDS             43717..46845
                     /locus_tag="IWO17_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ0289259.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46864..50058
                     /locus_tag="IWO17_000361"
     CDS             46864..50058
                     /locus_tag="IWO17_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0289260.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50204..50911)
                     /locus_tag="IWO17_000362"
     CDS             complement(50204..50911)
                     /locus_tag="IWO17_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0289261.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51676..52074
                     /gene="spxA"
                     /locus_tag="IWO17_000363"
     CDS             51676..52074
                     /gene="spxA"
                     /locus_tag="IWO17_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ0289262.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        17132 a         9077 c        10803 g        15198 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 ttgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatctcaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggctt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg aaactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccctggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttat tttacttgaa ctcattgact gttttccctt tattttaaac
    23041 attataaaga ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa
    23101 aaaaacctgc cgatagttgg caggtcgtct acgtgttaag ctttggtgtg gtctttagct
    23161 ggcaattggt tttctaaatc agttcggaca acaagcacat cgcaagctgc gtttctaata
    23221 acatattcag aaacagaacc aataaataaa cgttctacag cattcaatcc tgtggctcct
    23281 aacataatca aatctacttg attatcttct gggatttctc tggcaataat ttgttttggt
    23341 gagccatatt ctacgacaga ggttacatta ttcaagccag ctttcttcgc attgctttca
    23401 taatctgcca atgtttgctt tgccatttca gtagcttgtt ctgcaagcat tccatcaaag
    23461 gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg ctaatagaag ttctccgtta
    23521 tttcggatag ctacattgac cgccttttta aaagctaatt cagcttcatc agaaccatct
    23581 acggcaacca tgattttttt gtattgttgt aacataagca ttcactcctc aagtcaatcg
    23641 tattgtactc ttttattgta gaacacttta gggaaaaaga gaattattta ctgctttggt
    23701 tcagcaattt tttttgcaaa tcggatactg aaggtggcag aaacaataat tacgatcagt
    23761 aaaaagacca aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc cattacgcct
    23821 aaaacagcat atgcagcacc gtaaaattgt aggaatcctt gatttttatc atttttgttg
    23881 atcaatacaa aaaagatatc ctgtttatta aataaataat agctaacgaa taataatact
    23941 aaagtggcga taatcattaa aatcctaatc atgaacaaat cctccaaaat aagcaacaaa
    24001 tagaaaaagc aatttgccaa gagcaaattg ctttattctt taaaagatgg taatctccgt
    24061 taatgccgtt gtttgtcctg tagtattgat cccgcgaatt caagcaggtg ggtcccatgg
    24121 tcgtagcttc gaactaccaa atgataaggc atgttaccag cagtgacaca agacgggtcc
    24181 caagatatca ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa catcacagat
    24241 ttcccacctc tatagtagca tatgaattta aaaagagcaa tgagagagct cgcagaaaca
    24301 gaaccgtttt cttgtctagt tttttggagg atgctttttc cactctttta tacggtaata
    24361 ctgttgaccc agagcctgtt catatttccc tgtatctttt ggctgataat actgagcatt
    24421 cttcagttta tctggtaaat attgctgatt gacccaagcc tgatcaaaat gatgaggata
    24481 ctgataaccc acgccacggt tgagtgattt cgcacctttg taatggctat ctctcaaatg
    24541 atcaggaaca tctccagctt tgccctctcg aatatcagca agtgctgcat ctaatgccat
    24601 gtaggcagaa ttggattttg gcgacaagca aagatcgacg acgacgtctg ctagtgggat
    24661 acgtgcctca ggcaatccca atttttcagc agctaataca gcgttcactg tacgagccgc
    24721 agcagcagga ttacctaaac ctatatcttc ataaccgata accatcagac gccggcaaat
    24781 acttgccaga tcacctgcct caactaaacg ggcgagataa tgaagtgcag catccacatc
    24841 acttccacga atcgattttt gaaaagccga aataacgtca taatgagcat ctccattttt
    24901 gtcatgggta agagcttttc tttggacaca ttcttcaatg atcgacagtg taagatgaat
    24961 ccttccttct ttatctgaag gagtcgatag tgtagccagt tccagcccat tcaatgcact
    25021 ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat
    25081 tgcttgttgt ccaaggccgc gttctttatc ccttaaagca tgttcgactg ccaactggat
    25141 gtcttgttct gttaatggtt tgacttcaaa gatctgtgta cggctgcgga tcgctggatt
    25201 gatggtaata taaggattct cagtagtcgc tccgatcaag atgattcggc cactttctaa
    25261 atgaggaagc agaaaatcct gttttgtttt atctaatcgg tgtacttcat ccaataagag
    25321 gatcactgta ccactcattt ttgcttcttc cgctacaact tgtagatcct tttttgaatc
    25381 tgtagcggcg tttagcatgc gaaatgcata attagtagag ccggcgatcg cacttgcgat
    25441 actcgttttt ccagtaccag gaggtccata tagaatcatg gaagagagca tgcgagcatc
    25501 gaccatgcgg cgtatgattt ttccttcccc gactagatgc tgctgaccaa cgacttcatc
    25561 gatcgttcga ggtctcatgc gataagccaa tggctgttgc ataggatttt cctccttctt
    25621 gtttttacgt cccttaatta tatcatattt agcaagatgc ctagaaagaa cagccttctg
    25681 ttgttttgtt ttcatagctt atccaactaa tattgtgtat gataaagtag gtgagaaaaa
    25741 tgacggataa acaaaagata gatgaatttt tttggaataa aacgacgcca gaagtggcac
    25801 gggatttatt ggggatgtat ttggagcatg acacaccaga aggaagatta gctggatata
    25861 ttgtagacgc ggaagcatac ctaggtccag aagacgaggc cgcccatagc tatggtttaa
    25921 gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac ctttacacga
    25981 tgcatactca tcggattttg aacatcatta ctcagccaga agggattcct caaggagtaa
    26041 tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa
    26101 aaactggtcc agatatcagc aacggtccgg gaaaattagt ggaagcatta gcaattcctc
    26161 aagaacttta tggtcagtct atagcagata gttcgttacg cttagttttt gagaaaaaaa
    26221 agacaccaaa aaagatcaat gctttgccta gaatcggtat cccaaacaaa ggtgtatgga
    26281 cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa
    26341 accaaatcga gaaagattgg ggttggagga aagaaaatga aaaagaagga tctattaaca
    26401 tatttagatg aaataattga aaaacaagtg acagactatg atgtagcttt agattgggat
    26461 acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg cagaaaataa agcacaattt
    26521 gagattgatg atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc
    26581 gtacttcttt ttaatcctca gaaatctgtt tttgatgaac gcaattatct agcagtaatc
    26641 ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg attctttagt ggaatatcta
    26701 aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt ttttggatga agacgatgac
    26761 gaagctgtat tcgagcttca ctgggataat caacaactag cagaattagt agaagtaaaa
    26821 cagcaaaatg aagcagatac ttatttaccg tacccaagtt attaaggagt gacagatatg
    26881 aaatggaatg aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt tgctaatatt
    26941 ttgatggagg caggggcgag tggtgtcgcg attgaagatt ctttagatgt tgaacatttt
    27001 cagtcggatc aatatggtga gattttagat aaaaatgatt ttacatcgct gaaagaaggc
    27061 gcactagtca tggcttattt tccggaaacg actttcttac cggaaatctt gccttttatc
    27121 aaagatcgta tcacacaatt acctgagttt ggtctagcta ttggaaaaaa taaagtaaca
    27181 gttagtgaag tagaggaaag caactgggca acagcctgga agaaatacta tcatccggtg
    27241 cgtattacgc gaatgctgac ggttgcgcca agctgggaaa actaccagac agaagatcct
    27301 ctagaaaaaa ttatcacact agatcctggt atggcatttg gcacaggcac tcatcccacg
    27361 acttccttga cattgacagc actagaaaca gtcttgcgtg gtggtgaaac attgctagat
    27421 gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc
    27481 tatgcctttg atttagacga agtagccgtt cgttcggcga aagaaaatat ggatatgaat
    27541 gaaatagcca aagatgtcca tgtatcagct aacgatcttt tgacaggaat cgagattgaa
    27601 agtgatgtaa tcgttgcgaa cattttagca gatatcatct tattgatgat cccagatgct
    27661 tggcgtttac tgaagcaaac aggcacgctg atcgtttcag gaatcattga agagaagaag
    27721 caaatggttc tggatgccat gttagaacaa gggtttatcg tggatcaaat tttccagcaa
    27781 aaagactggt atgcaattat tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt
    27841 ttaaaagaat catatgaatc aaaagactat tttgttttag aaggggaaaa ctatcaccac
    27901 attgtacggg ttatgaggat ggaacccgag gatgaagttt ttttggcatt caatgatcaa
    27961 atagccattg ttgcagaaat cacggagatt actgaagagg ctgtttatct gaaagaaatt
    28021 aaaaaagaag aaaatcaaaa ggaactgcca agttcgatta ccatagcgag tggctatcct
    28081 aaaggggaaa agctggattg gatcgttcag aaatccactg aattaggagc gcaagctttt
    28141 cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga
    28201 caacaacggc ttgaaaagat tgctcaagag gcagcggaac aatcccatcg gcagatcgtg
    28261 ccatcagtag agttattgga aacgaaacaa gatttatttg agcggctaga tgattatgac
    28321 gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa
    28381 attcttagtg aaacaaaacc agatagtcga atcttagctg tctttggacc agaaggtgga
    28441 ttatcccgcg atgaagtcga ttattttgta gaacacaaag ggatactttg tggattagga
    28501 ccaagaatcc ttcgtacaga aacagcacct ctttatttat taagtgctgc tagctatcaa
    28561 tgggaattaa tgaatccttc tttcagctaa cgttcttcat taataaaaga gtatcgacag
    28621 aaagaattga aaaaatagtc aacaatttgt ataatagaaa caattattta tacaatggat
    28681 acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac
    28741 tgcacggatt ttgggacaat tatcaaacag atatttgtcc cgattcccgt tttaccgaaa
    28801 aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat
    28861 aaagctcgtt agtcaataca tgggtcccga acatgtagca tttgttgaga aagcatgcga
    28921 atacgcgact gctgcacatg acggacagtt tagaaaatca ggcgaaccct acattattca
    28981 tcctatccaa gtcgcaggta tattagcaga tttaaaaatg gatccccata cagtggctac
    29041 aggcttctta catgatgttg ttgaagatac agaaatcact ttagaagatc tgagagaaga
    29101 atttggcgat gacgttgcta tgttagtgga cggcgtaacc aaattaggga aaataaaata
    29161 taaatcccac gaagagcagc tagcagaaaa ccaccgaaag atgctgctag caatggctca
    29221 agatttacga gttatcatgg tcaaattagc tgatagacta cataacatgc gtacgttgaa
    29281 gcacctgcga gaagataagc aaagaaggat cgctcaggaa actttagaaa tctatgcgcc
    29341 tcttgcacat cgtctaggga tcagccggat caaatgggaa ttagaagata cagcacttcg
    29401 ttatctaaat ccaaaacagt attaccgtat cgtccacttg atgcagacga agagagaaga
    29461 acgtgaaaaa tacgtaagtg gtactgttga agatattcga atagcgacag aagagctggg
    29521 gatttttgca gaaatctatg gacggccaaa acacatttat tcaatctatc gtaaaatgaa
    29581 agatcagaaa aagcagttca acgaaattta tgacttgcta gctattcgag tgatcgtaga
    29641 ttcaatcaag gattgttatg ctgtattagg agcaatccac acaaaatgga aaccaatgcc
    29701 cggcagattt aaggattata ttgccatgcc aaaagctaat atgtaccaat ctttgcatac
    29761 taccgtgatc ggtccggcag gaaatccggt agaaattcaa atccgaacac aggaaatgca
    29821 tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa
    29881 agtagaacca gatggtatga cgaaacaatt aagctggttc catgagatac tcgaacttca
    29941 agacgaaagc tatgatgctt ctgaatttat ggaaggcgta aaaggagata tctttagtga
    30001 taaagtctac gtcttcacac caaaaggaga cgttactgag ttaccaaaag gatccggacc
    30061 attggacttt gcatacagta tccatacaga tatcggtaac aaaaccactg gtgcaaaagt
    30121 aaatggcaaa atggtgcagc ttgattacaa attgaaaaac ggagatatca ttgagatcat
    30181 gacttctcca aattcatttg gcccaagtcg cgactggttg aaattagttg ctactagcaa
    30241 agcaagaaat aagatcaaac gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa
    30301 aggccacgaa tccgtggtca aatgtattac agatctagga tttacgccta aagatatttt
    30361 gacgaagaac aaactgcaag aagcactcga tcgttttaat tatcaaacag aagatgatct
    30421 ctatgcagct gtagggtatg gagaagttag ccccttgacg atggccaatc gtctgactga
    30481 aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa
    30541 tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca
    30601 aggggtagaa aacttactaa ttcgtatcag tcgctgctgt aatccgattc ctggtgatga
    30661 tatcgttggt tatatcacta aaggcagagg gatatccatt catcgtcgag attgtccgaa
    30721 tgttcagcct gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat gggaagatac
    30781 atcgaataca cgaaaagagt atgatgcaga tttggaaatt tacggctata atcgttcagg
    30841 cttattgaat gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca acagcgttga
    30901 agcacggacg aataaagata aaatggcgac gatccatttg acggttggta tccagaattt
    30961 atcccaccta aagagtatcg tggataaaat caaagcagta cctgatgtct acagtgtacg
    31021 ccggacgaat ggataggagg acagatatga gagcagttgt tcaacgggta agtaaagctg
    31081 ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta cggcctactt gttttattag
    31141 gcattcatga aaccgatacg caaaaagatg tggactatct ggtaaaaaaa attgcacaaa
    31201 tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag tgtagaagat gtgggaggac
    31261 aaattttaag tatctctcaa ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa
    31321 gctttgtgga agccgcacga ccagaaacag ctattccttt atacgaatca ttcaatgaag
    31381 gaatcagaag tcgcggcatc acagtcgcta ccggacaatt cggagcagat atgtcggttt
    31441 ctctcgtcaa tgatggacct gtaacgatca ttattgattc taaaaacaaa taaagtaatc
    31501 tggttttcac tagtaaaaac tatgaaacac gtcgcttaag ttagactgat aaactctaac
    31561 ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta
    31621 agcttaaatt atacaagtta ttttttatag gaggttgctg aagatgaaag aagtaccagt
    31681 agtagatatc aaaatcacag atttaaaaaa attgtatcaa aaaggataca acatgttgga
    31741 ggagttacgc catgaagcgg acgctcccgt cgtcaaagca aagattttca ataaagaagc
    31801 tatcacgatc tacggatcat cagcagcaaa agtattttat gatccaagga atttcaaacg
    31861 aaaaggtgct atgccaaaat tagtattgaa gacacttttt ggtcaaggtg gagtacagac
    31921 tttggatgga gctgctcatc accatcggaa aaatattttt atggatctaa tgacaccgga
    31981 aagaatggaa gattatcatc gtatcttaga taagaatctg acacaagcac ttgaagcaca
    32041 gcatggtcaa tttgaactat ttgatttaag taagatggtt ttctttacat ctatttgcga
    32101 gtgggcaggt atcaatttat cagcgatttc taaagatgag gtcgaaaaat tagcagaata
    32161 tcaaatctct atgatcagcg gaacgtttac ttctccgatt gatcacataa aaggtgtcga
    32221 gaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt gaagaagctc gacaaaatcc
    32281 tgtggctgga aaagaaaatg tggcattata tgcttttgcc aatgccacag atttagatgg
    32341 tcagctttta cctctggaag tggcagctgt tgaattattg aacatcatcc gtccaacggt
    32401 tgctctaact gtgtgggcgg cattgatggg acatgcactt ttcagtagac cagatttgta
    32461 tcagcaatta aaaaatgact tttcgacgtt acaagatccg tttattcaag aaatgagacg
    32521 ctattatcct ttcttcccaa tgctacctgc gatttcgctg aaagaagtag aagtagatgg
    32581 ttatcgtata ccagaaggca gttgggttat tttagattta tatggaacag accatgatga
    32641 acgaacagtt gaagcgccag attcctttat gatcaaacgg tatgtaggga aagcaaaaga
    32701 tatttcctat aaagaagaat atgagatgat tgcacaaggc gggggaaatt ttcgacaaat
    32761 gcatcgctgt gcaggagaat ggatcactct tcatagtcta cgtgtgtttt ccgatcagct
    32821 agtaaataag tttgagttct ctgtacctga acaagattgg acgattccat tcaatcagtt
    32881 tccgacttat ccgaatagcc gagcgctact ttacaagaat tagattagca taaacttatc
    32941 gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt catattacta taaattgata
    33001 aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg tttgataatc ttcattggat
    33061 aaatgcaaat ctaaagcttg gacattgtca gatacttgct ctggacgctt agctccaggt
    33121 ataactacag acactcgtgg attcttcatg taccaagcta aaacaagttg cgcaggagtg
    33181 gcttggtatc ttttagcaat ggggcgaagt acatcgacag cggttaaaat ttctctgaaa
    33241 cgttctcctt ggaaatctgg atttcttttt cgaggatctc cttcaccaaa ttgtttttcc
    33301 tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa
    33361 ctgattttgt ttttttcaag ataagggaat aattcttttt cagcctgccg atggatcaaa
    33421 ctgtatttat cttctactac atcgacatat ccatctgcat tcgcttcttt caattgttct
    33481 aacgtgaagt ttgacacacc gactgctcgg atttttcctg cttcttttaa ttcgtgcaat
    33541 gtagcaactg attcattttt tggtgtgctt tcatccggaa agtggatata aaaaatgtcg
    33601 atgtaatcag tttgcagacg ttttagtgca tcttccaccg attgtttgag aaactcagga
    33661 gaattatcaa atgttcgccc tttatttggt acatgagcag cttttgtagc gatgacgaca
    33721 cgtgagcgat catattcttt ttccttcagt acctcgccaa tcagttcttc ggaacggccg
    33781 tttccgtaag cataagcggt atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact
    33841 acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt
    33901 gctcctaatc caagctgttc tgcgtagact tgtgaatgtc cgattcttat ttctgccata
    33961 gctgattcct cctttttgtc tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt
    34021 tttgtgaggg gaaatagtca gatatctctt gactttgaga ctggttttta gtaggttata
    34081 agtaatatcg aatgtcaatg aaagagcgag taagtttttg aaactttaca agagaggatc
    34141 atcgaggctg aaaatgatct aaagtttcat tagcctaaag acactctgga gatggacgag
    34201 tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga aagagaaatc tttcaaaatt
    34261 aggtggtacc gtgtatgaat ttcattcacc cttgttcatg atgaacaagg gtgatttttt
    34321 tatttacata gctaagaaag ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa
    34381 atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat
    34441 tattcagaga ttatcagtat aatgaaatgc gtacaccgat ttttgaacac tatgaagtga
    34501 tatcccgaag tgtaggggat acaacggata tcgtttcaaa agagatgtat gatttttatg
    34561 acaaagggga acgccatgtg acgcttcgac cagaaggtac agcaccaatc gttcgttctt
    34621 ttgttgagca taaattgttc ggcccagagt atgcgaaacc gttcaaaact ttttatatgg
    34681 gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc catcaaatcg
    34741 gagtagaagc atttggatca gaaaaccctg caactgatgt ggagagcatg gtgatggcac
    34801 ttgatttctt caaacagttg gggatcacgc aaatccggct agtcatcaat tcattaggtg
    34861 ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga ctatctttta ccttttgaat
    34921 cagagctgag tgaagactct aaacgccgcc ttcatgaaaa tccattacgc gtattggaca
    34981 gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc atctatctta gattatctaa
    35041 gcgagcctgc tcaaaaacat tttgataccg ttgtttccat gctggatgca ttggctgttc
    35101 cttatgaaat cgacagtaat atggttcgcg gactagatta ttacacacat acgatttttg
    35161 aaatcatgag tgacgcacca aaaatggggg cacaagcaac gatttgcgca ggcggacgtt
    35221 atgacaatct agtggaagaa ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg
    35281 gtatcgagcg attactcttg actatggagg cagaggaagt agttatccct gcatttaatg
    35341 agttggatgc ttacgtggtc gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg
    35401 tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa
    35461 aagcgcaatt taagacagca gataaagcga atgccaaatt agtattggtg atcggtgaag
    35521 atgagcttgc tagtcaaacg attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt
    35581 ttccattaga agagatatat gaacattttg atgaagtcta tgatgaaatg actttattcg
    35641 agtaggagga aaaaaataaa tggcacaaag aacgatctat tgcggattag tttccgaaca
    35701 acaattagaa caaacagtta cattaaaagg atgggtacaa aaaagacgag accttggggg
    35761 agtaatcttt atcgacctac gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga
    35821 aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt
    35881 gactggggaa gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga
    35941 attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca
    36001 aattgaagat gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt atttggatct
    36061 gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat gcgacaacaa aagcgattcg
    36121 tcattattta gatgataatg actttctcga tatcgaaaca ccttacctag ggaaatctac
    36181 accagaaggc gcaagagact acctagttcc ttcacgtgtc catgctggac atttctatgc
    36241 gttgccgcaa tctcctcaat tgtttaaaca attgctgatg aatgctggat tcgaccgtta
    36301 ttatcaaatc gttcgttgtt tccgcgatga agatttacgt ggagaccgtc agcctgaatt
    36361 tacgcaagta gatattgaaa caacgttctt aacagcagaa gaaatccaaa cttacacaga
    36421 gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc
    36481 tcgtatgacc tatgatgaag caatggctcg ctacggcagc gacaaaccgg atacacgttt
    36541 tgatatggaa ttgatcgatc taagcgatac agtcaaagaa gtagaattca aagtcttcca
    36601 aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg
    36661 ctactcacga aaagacatgg atcagttagg tcaatatgta ggacaattcg gagctaaggg
    36721 gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt ccaattgcta aattcatggg
    36781 agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt
    36841 tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga cacgtttagg
    36901 aaaagaatta ggactaattg atgaatcgaa attcaatttc ttatgggtaa ctgattggcc
    36961 acaatttgaa ttttcagaag aagaaggtcg atatgtctct gctcaccatc cattcacgat
    37021 gccaaaagaa gaagatatcc cgttattggc tgaagaccct gcaaaagtct atgcagaagc
    37081 ttatgatatc gtcttgaacg gttatgaatt aggcggaggt tctatccgta ttcatactcg
    37141 tgagcttcaa gaaaaagtac tagaaactct aggctttaca aaagaacaaa tggaagaaca
    37201 atttggtttc ttgttaacag ctttagatta cggattccct ccacatggcg gtattgcatt
    37261 aggattggac cgttttgcaa tgttattagc cggagaagat aatattcgtg aagtcattgc
    37321 ctttcctaaa aacggaaaag cagcagatgt gatgagtgat gcaccaagta tggtttcgcc
    37381 tttacaattg ttcgaattga atattgatgt aacgaatata gaagaatagc cgataaaaag
    37441 gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt gatttaattg aaaaacactt
    37501 tataatgaag ccataacata taaagcgggg cttttccatg aaaaaaacat tcgaaaattg
    37561 ggcttatcat gattacacga ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc
    37621 agtcgcattg aatttctttt atcagccagg acatatttat tcaagtggta ttactggtct
    37681 agctcagatt ttgacgactt tgtcaaaatc actgatagga ttcgaagtac cggtatcaac
    37741 aactttgtat ttactgaata tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa
    37801 gtttacaatt ttcactttta tcacagtcac tttgacctcc ctttgtatcc atattttacc
    37861 ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg tttggggggg ctgtgatggg
    37921 actaggtatt gggctagcgt tgagaaacgg cctatcatcg ggcggtctag atatcgtgag
    37981 tatcacgatt cgtaaaaaaa caggaaagtc gattggttct atttcgatct acttcaatgc
    38041 attgatcgtt tttgttgccg gaattctatt tggctggcaa tatatgtttt acagtacgtt
    38101 gtctattttc gtcagtggaa aagtaactga tgtcgtttat actaaacaaa agaagatgca
    38161 ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa actgtatgcg
    38221 tagagggatc acgatcatca atgaagcaga aggtgcttat aagcatgata agcagacagt
    38281 attgatgacg atcgttacac gatacgaact gcctctactt agagaagcaa tgaaagcttc
    38341 tgatccacaa gcttttgtaa gtattgcaga aaacgtccag atacttggtc gtttctatga
    38401 ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag acagacgttc
    38461 gtttgtctgc agtttttttc taccatagta gaatcagcaa agaaaaactg cttttttatt
    38521 tgaaattttc ttaaagttga aaaaaagcat gtttattttt ttaaatttat gggatgatag
    38581 aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat
    38641 tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa
    38701 aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta
    38761 tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt
    38821 tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa
    38881 aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt
    38941 ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc
    39001 ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg
    39061 gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag
    39121 cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg
    39181 ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct
    39241 tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca
    39301 ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt
    39361 taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc
    39421 ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg
    39481 gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg
    39541 gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct
    39601 atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata
    39661 ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg
    39721 aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc
    39781 aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc
    39841 caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg
    39901 taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag
    39961 aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt
    40021 acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg
    40081 attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag
    40141 atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac
    40201 aagatttgtt gaaagacact gttctgattg gactaggaac gaatggctca ttctcggaag
    40261 ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc
    40321 gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa
    40381 aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat agtgactggt
    40441 tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca
    40501 gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat
    40561 gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt
    40621 accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat
    40681 aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc
    40741 ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag
    40801 tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat
    40861 atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca
    40921 tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt
    40981 ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt
    41041 gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct
    41101 tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca
    41161 tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca
    41221 tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa
    41281 taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct
    41341 ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt
    41401 cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt
    41461 ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt
    41521 ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg
    41581 gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga
    41641 agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa
    41701 aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg
    41761 aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt
    41821 agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat
    41881 actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc
    41941 caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat
    42001 attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa
    42061 aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac
    42121 aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa
    42181 aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag
    42241 agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga
    42301 aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt
    42361 gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg
    42421 gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac
    42481 gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat
    42541 ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta
    42601 catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag
    42661 cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg
    42721 attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat
    42781 gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat
    42841 cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt
    42901 ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc
    42961 tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga
    43021 acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa
    43081 aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa
    43141 acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat
    43201 tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat
    43261 agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc
    43321 gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt
    43381 gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat
    43441 cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac
    43501 cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa
    43561 caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca
    43621 ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa
    43681 acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt
    43741 aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg
    43801 ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc
    43861 ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg
    43921 tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc
    43981 tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa
    44041 aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa
    44101 atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa
    44161 cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca
    44221 agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc
    44281 aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa
    44341 gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg
    44401 tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg
    44461 gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac
    44521 tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa
    44581 aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat
    44641 gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa
    44701 atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat
    44761 tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa
    44821 gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt
    44881 aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag
    44941 aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta
    45001 ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat
    45061 gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata
    45121 agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa
    45181 cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt
    45241 cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata
    45301 caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg
    45361 gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat
    45421 gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata
    45481 caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata
    45541 acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa
    45601 agcaagatgg ccaatgcaag aatagagctg gaagaaataa gacaacaagc agaaaagctc
    45661 gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa
    45721 ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta
    45781 gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt
    45841 gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat
    45901 cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata
    45961 gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca
    46021 caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa
    46081 ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca
    46141 gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa
    46201 gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt
    46261 ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt
    46321 tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag
    46381 gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat
    46441 cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa
    46501 atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt
    46561 gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa
    46621 gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa
    46681 gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc
    46741 ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat
    46801 acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta
    46861 actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa
    46921 ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg
    46981 ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa
    47041 tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta
    47101 cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa
    47161 gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat
    47221 cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag
    47281 tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg
    47341 aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa
    47401 acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa
    47461 gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg
    47521 aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa
    47581 gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa
    47641 caaaacgatc aaggaataca attttccgat caaaacaaac ctttatcatt caaagtggaa
    47701 aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat
    47761 tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt
    47821 tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa
    47881 aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta
    47941 gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt
    48001 ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc
    48061 tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac
    48121 acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa
    48181 actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa
    48241 ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat
    48301 ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat
    48361 attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg
    48421 ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa
    48481 gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa
    48541 ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc
    48601 aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga
    48661 taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt
    48721 tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg
    48781 ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa
    48841 agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg
    48901 agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa
    48961 gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat
    49021 ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc
    49081 aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt
    49141 gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg
    49201 attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt
    49261 gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg
    49321 ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat
    49381 ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag
    49441 aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct
    49501 cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta
    49561 ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg
    49621 ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc
    49681 agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat
    49741 gtttttctga tttccttaaa ggctggggga actggattga atctcacggg agcagatacg
    49801 gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca
    49861 catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt
    49921 gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa
    49981 ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta
    50041 ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc
    50101 taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat
    50161 cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct
    50221 tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt
    50281 caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta
    50341 gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt
    50401 ctttctcagc cagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa
    50461 tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct
    50521 gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat
    50581 cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt
    50641 tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt
    50701 ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga
    50761 agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa
    50821 caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat
    50881 catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga
    50941 ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata
    51001 aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt
    51061 ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt
    51121 agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcgaaaga tttatttttt
    51181 taatacagtc tcaatataac tattcaaaca taaaataacg ttatttgatt tatttgttta
    51241 tagtaaattt tatttagtaa tcattgcttt aaaatactac gcttatataa cagagaaaat
    51301 aagcgttata ataaagaaca gtattattta aaatgacaga aaacattact attaaagaaa
    51361 taaaaaacat aaaaaatctt aagaaatcgg tttcatttag cttttgagat aaaataatag
    51421 tacagtttta tacgctgttt catatgttta gaaaatttga attagatttg aaaactctaa
    51481 tttttaaatg ataaaaaaag cgagaatgct ggttttactg tatattaacc ttagataact
    51541 ttcatttttt taaaaaaatt atgaaaaaaa ggtagctatt taaaagttga ttatgtataa
    51601 tagaaacaga tgggagatta ttatgatctt ctgttacaaa aaagtgattc ttatcacgaa
    51661 aaggagtggt gcacgttgtt aacattatat acttccccaa gttgtacttc ctgcagaaag
    51721 gctcgtgcgt ggttgcaaga acatcagatt ccatttgttg aacgtaatat tttttcagaa
    51781 ccattgaaca gttctgaatt aaaagcgatt ttacaaatga cagaagacgg aacagaagaa
    51841 atcatctcga cacgttccaa agtattccaa aaactgaaca tggatttaga tgatctgccg
    51901 ttacaagaat tattggaact tgtgcaaaac aatcctggat tgcttcgccg tccaatcatg
    51961 attgatgata aacgtctgca agttggtttt aatgaagatg aaattcgtcg gttcctaccg
    52021 cgcgatgtac gacaattaga attacgtcaa gcacaattga tggctggatt atagaaggat
    52081 aatacgaaac ttggaaaaga ctggctcttt gtcaaatgat gttggtaaag agaaatctta
    52141 gaataatttg gacagaacag aaagaagaaa acctctttct gttctgttta tttggttaaa
    52201 aatgttttgc
//
LOCUS       DACECR010000007        51762 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.007, whole genome shotgun sequence.
ACCESSION   DACECR010000007 DACECR010000000
VERSION     DACECR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.007"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(132..935)
                     /locus_tag="IWO17_000364"
     CDS             complement(132..935)
                     /locus_tag="IWO17_000364"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ0289263.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(955..1797)
                     /gene="kduI"
                     /locus_tag="IWO17_000365"
     CDS             complement(955..1797)
                     /gene="kduI"
                     /locus_tag="IWO17_000365"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAQ0289264.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(1813..2820)
                     /locus_tag="IWO17_000366"
     CDS             complement(1813..2820)
                     /locus_tag="IWO17_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ0289265.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(2827..3471)
                     /locus_tag="IWO17_000367"
     CDS             complement(2827..3471)
                     /locus_tag="IWO17_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0289266.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(3571..4329)
                     /locus_tag="IWO17_000368"
     CDS             complement(3571..4329)
                     /locus_tag="IWO17_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAQ0289267.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(4456..5607)
                     /gene="fucO"
                     /locus_tag="IWO17_000369"
     CDS             complement(4456..5607)
                     /gene="fucO"
                     /locus_tag="IWO17_000369"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAQ0289268.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(5689..6000)
                     /gene="rhaM"
                     /locus_tag="IWO17_000370"
     CDS             complement(5689..6000)
                     /gene="rhaM"
                     /locus_tag="IWO17_000370"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAQ0289269.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(6002..6847)
                     /gene="rhaD"
                     /locus_tag="IWO17_000371"
     CDS             complement(6002..6847)
                     /gene="rhaD"
                     /locus_tag="IWO17_000371"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAQ0289270.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(6861..8117)
                     /gene="rhaA"
                     /locus_tag="IWO17_000372"
     CDS             complement(6861..8117)
                     /gene="rhaA"
                     /locus_tag="IWO17_000372"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAQ0289271.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(8114..9574)
                     /gene="rhaB"
                     /locus_tag="IWO17_000373"
     CDS             complement(8114..9574)
                     /gene="rhaB"
                     /locus_tag="IWO17_000373"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAQ0289272.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            9727..10596
                     /locus_tag="IWO17_000374"
     CDS             9727..10596
                     /locus_tag="IWO17_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0289273.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(10722..11621)
                     /locus_tag="IWO17_000375"
     CDS             complement(10722..11621)
                     /locus_tag="IWO17_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0289274.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(11786..12610)
                     /locus_tag="IWO17_000376"
     CDS             complement(11786..12610)
                     /locus_tag="IWO17_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0289275.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(12621..13916)
                     /locus_tag="IWO17_000377"
     CDS             complement(12621..13916)
                     /locus_tag="IWO17_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAQ0289276.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(13932..14411)
                     /locus_tag="IWO17_000378"
     CDS             complement(13932..14411)
                     /locus_tag="IWO17_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ0289277.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(14417..15445)
                     /locus_tag="IWO17_000379"
     CDS             complement(14417..15445)
                     /locus_tag="IWO17_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAQ0289278.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(15462..16568)
                     /locus_tag="IWO17_000380"
     CDS             complement(15462..16568)
                     /locus_tag="IWO17_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0289279.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(16797..19394)
                     /gene="adhE"
                     /locus_tag="IWO17_000381"
                     /gene_synonym="adhC"
     CDS             complement(16797..19394)
                     /gene="adhE"
                     /locus_tag="IWO17_000381"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ0289280.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(19699..20079)
                     /gene="yajC"
                     /locus_tag="IWO17_000382"
     CDS             complement(19699..20079)
                     /gene="yajC"
                     /locus_tag="IWO17_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ0289281.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(20176..21321)
                     /gene="tgt"
                     /locus_tag="IWO17_000383"
     CDS             complement(20176..21321)
                     /gene="tgt"
                     /locus_tag="IWO17_000383"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ0289282.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            21539..22237
                     /locus_tag="IWO17_000384"
     CDS             21539..22237
                     /locus_tag="IWO17_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0289283.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(22405..25044)
                     /locus_tag="IWO17_000385"
     CDS             complement(22405..25044)
                     /locus_tag="IWO17_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0289284.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(25663..26694)
                     /gene="queA"
                     /locus_tag="IWO17_000386"
     CDS             complement(25663..26694)
                     /gene="queA"
                     /locus_tag="IWO17_000386"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ0289285.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            26887..28062
                     /locus_tag="IWO17_000387"
     CDS             26887..28062
                     /locus_tag="IWO17_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0289286.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            28066..28707
                     /locus_tag="IWO17_000388"
     CDS             28066..28707
                     /locus_tag="IWO17_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0289287.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            28704..29621
                     /locus_tag="IWO17_000389"
     CDS             28704..29621
                     /locus_tag="IWO17_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0289288.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            29618..30289
                     /locus_tag="IWO17_000390"
     CDS             29618..30289
                     /locus_tag="IWO17_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0289289.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(30830..31318)
                     /locus_tag="IWO17_000391"
     CDS             complement(30830..31318)
                     /locus_tag="IWO17_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ0289290.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(31519..31785)
                     /locus_tag="IWO17_000392"
     CDS             complement(31519..31785)
                     /locus_tag="IWO17_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0289291.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(31785..32048)
                     /locus_tag="IWO17_000393"
     CDS             complement(31785..32048)
                     /locus_tag="IWO17_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0289292.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(32041..32313)
                     /locus_tag="IWO17_000394"
     CDS             complement(32041..32313)
                     /locus_tag="IWO17_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289293.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            32524..32709
                     /locus_tag="IWO17_000395"
     CDS             32524..32709
                     /locus_tag="IWO17_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0289294.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(32851..34674)
                     /locus_tag="IWO17_000396"
     CDS             complement(32851..34674)
                     /locus_tag="IWO17_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ0289295.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(35272..37977)
                     /locus_tag="IWO17_000397"
     CDS             complement(35272..37977)
                     /locus_tag="IWO17_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ0289296.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(38283..39230)
                     /locus_tag="IWO17_000398"
     CDS             complement(38283..39230)
                     /locus_tag="IWO17_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ0289297.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLEASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            39420..39689
                     /gene="rpsN"
                     /locus_tag="IWO17_000399"
     CDS             39420..39689
                     /gene="rpsN"
                     /locus_tag="IWO17_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0289298.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(39865..40227)
                     /locus_tag="IWO17_000400"
     CDS             complement(39865..40227)
                     /locus_tag="IWO17_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0289299.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(40238..41359)
                     /locus_tag="IWO17_000401"
     CDS             complement(40238..41359)
                     /locus_tag="IWO17_000401"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ0289300.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(41375..41725)
                     /locus_tag="IWO17_000402"
     CDS             complement(41375..41725)
                     /locus_tag="IWO17_000402"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ0289301.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(41855..43366)
                     /locus_tag="IWO17_000403"
     CDS             complement(41855..43366)
                     /locus_tag="IWO17_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0289302.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(43688..45040)
                     /locus_tag="IWO17_000404"
     CDS             complement(43688..45040)
                     /locus_tag="IWO17_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ0289303.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(45193..46269)
                     /locus_tag="IWO17_000405"
     CDS             complement(45193..46269)
                     /locus_tag="IWO17_000405"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ0289304.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            46465..48210
                     /locus_tag="IWO17_000406"
     CDS             46465..48210
                     /locus_tag="IWO17_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ0289305.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(48490..48927)
                     /locus_tag="IWO17_000407"
     CDS             complement(48490..48927)
                     /locus_tag="IWO17_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ0289306.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(48911..50284)
                     /locus_tag="IWO17_000408"
     CDS             complement(48911..50284)
                     /locus_tag="IWO17_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289307.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(50606..51094)
                     /locus_tag="IWO17_000409"
     CDS             complement(50606..51094)
                     /locus_tag="IWO17_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0289308.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(51116..51718)
                     /locus_tag="IWO17_000410"
     CDS             complement(51116..51718)
                     /locus_tag="IWO17_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0289309.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        14589 a        11283 c         9333 g        16557 t
ORIGIN      
        1 atttcttgga gttaaatgct tctgtcccag cctctttttt cgaataaatg atgtttaatc
       61 agctgtcctt cggtattcgt tcaatccgct gatttcacaa cctctccact aaacagtgta
      121 ctaaagctct tttaaggttg ttttccgata taggcaagta tgcctccgtc cacatacaag
      181 acatggccgt taacgaaatc agatgcgcta gaagccaaga agacagatgg tccagccaaa
      241 tcgattggct cgccccagcg tgctgctggc gtacgtccaa cgataaattg atcgaatgga
      301 tgacgttcac cgttttcttg tgtttcgcgt aatggagcag tttgcggtgt agcaatataa
      361 cctggtccaa ttccattaca ttggatattg tattgaccgt attcagaagc gatgtttttc
      421 gtcagcattt ttaatccgcc ttttgccgca gcataagcac tgaccgtttc gcgtcctaat
      481 tcactcatca tggagcagat attgatgatt ttaccgccgc ctttttcgat catatccgga
      541 ataactgctt ttgaaacgat gaatggggca tttaaatcca cgtcgattac ttggcggaag
      601 tctgccgcat ccatttcgat catcggtgtc cgtttgatga tccctgcatt gttgactaga
      661 atatcaactg aacctacatc ttctttgatc tgttgaacca tcgcttgaac cgcttcttca
      721 tcggtcacat cgcagacata accttttgca tcgatgcctg cttctttata atgcgctata
      781 ccttcatcaa ctgattcttg attcaagtta ttaaatacga tttttgctcc tgctgtagct
      841 aaagagcgtg caatctcaaa tccaatacca tagacagcac cggtgactaa tgctactttt
      901 ccatctaaac gaaacatatc catactaaat tccatcattt tctctccctt atgcttattt
      961 taattgatcc atttgcacca tatccatgtc tgtataggtg atattttctc cgcacattgc
     1021 ccagatgaat gagtaattgc ttgtacctac acctgaatgg atcgaccagc ttggcgaaat
     1081 aatggcttgt tcattgtcta caactaaatg ttttgtttca tctggtttgc ccatcatgtg
     1141 gaacacacgt gtatcttcat tggcataatc aaagtagaca taagcttcca tacgacgttc
     1201 atgtgtatga caaggcatcg tattccaagc gcttcctggt tctaaaatcg tataacccat
     1261 ttgtaattga cagctttcgc atacatttgg atggatgtat tggtagattt tacgttcatt
     1321 cagtgtcagg ccttcaccag tctccattgg ttttacttgg tcgatactga tttttacatt
     1381 tggatattta tgatgagctg gaacagaact aatatagaat ttagctgggt tttcagcatc
     1441 ttgagaagaa aatacaacat gtcgtgtttc ttttccgata tagtatccgt cttgtttttt
     1501 catttcttct ttttgcccat cgatctcgat gaatcctggt ccaccgatat tgatcacacc
     1561 tagttcacga cgctctaaga agtaatcaac acctagttct ttgtctaatg tgatttctaa
     1621 ttctttttca gtaggtgtca ctccaccaaa gatcattcga tcgttgtgcg tataagtcaa
     1681 actgattttc cctggttcaa ataccttttc tactaaaaat tcttttctca attgttccgt
     1741 tgaataatga cggatatctt ctggactgtg tgtataacgt gtttccattt cttgcatttt
     1801 cttctactcc ttctatcgaa tgatttttcc agagccgcat gctaaaaatt gtgcgacttc
     1861 ttcttgacta aattggttgc agtccccatg gatcgtatgc ttcaaggcag aagctgccgt
     1921 agcaaaatca atgatttctt gcggagacat attggataaa atcccatgga gcacgccacc
     1981 tgcaaaagca tctccgccac ctactcgatc cacgattggg gtgattctat ggacttggga
     2041 ttccacatat tcatggttca tccacaatgt ccccttcaat tcattttcac ttgctgaatg
     2101 aacggtacgc ttggtcgaat aaaacacctg aatattcggg aacttttcct gcattttttg
     2161 ataataataa attagaggtt gttcttctgc ttctggcgct ttctcgatac ctaacaaata
     2221 aagagcatcc atttttccag ctgagcagat atctactaaa ggtaaaagct gatggattac
     2281 ttcgctggct tcttgctgtg tccataattt tccacgatag ttgatatcga agctgatcag
     2341 gcagccagct ttttttgcag cttctatcac ctgtttcgtc attttttgcc attttttaga
     2401 caaagctggg gtgattcctg aaacatgaaa tatatctatt ccttggaata actcctctag
     2461 cgaccattcc aaatgatcca tttcagcaaa acttgatccg gcacgatcat agatcactga
     2521 ggcggcgcgt tcgccactac cagtttccat ataataagta cctagtcttg atccaccaga
     2581 taatacctgt ttcgtatcta ctccataaca ttgcaagtgc tttttgactg cttttcccaa
     2641 tgcgttatca ggtacttttg tagcaaaaat cgcttcatgt ccgtaattag ctaaagaaat
     2701 tgctacgttt gcttcccctc cgccataatg ggcagcgaat cgttcacttg aatcgatacg
     2761 ggttccctgc tctgtggaca atcgcaacat gatttctccg agtgtcacta cttttcccat
     2821 aagcagctac ctcttgattt ctttcatttt tgctgcatac tgttgcgcga cttccgttac
     2881 tttgtcaaaa tctcctgtag ctgcgggtgc taaaaggttc ccacctactc cgactgttac
     2941 gacaccagca tcgaaccact ctttcatatt gtccagactc acaccgccag ttggcatgat
     3001 attgacttga ggcatcggcg ccttgaaggc agaaatgata cttggtccat aagcgcttcc
     3061 aggaaatagt ttgacgatat ctaccccgct tttcagcgct gttttgatct cggtaatagt
     3121 catacatcct ggtaagtaag gaatttgata cagattgcac atctctgccg tttcttgatc
     3181 aaaggaaggg ctaacaatat actctgcgcc agctaagata gccaagcgag ctgtgacagc
     3241 gtctaatact gtgcctgcgc caatcactat ttctggttgg tctttgtaga atgacaaaag
     3301 ttctttgatt acttgatctg cttgagggac tgtaaaagtc aactcaatac cagttagacc
     3361 acctttgatg attgcgtggc aagcattcaa agcttcttct ttgctttttc cgcgtaccac
     3421 cgcaatcact ccggcgtttt tcaatcgaga taaaatatca atttttttca tcttctccac
     3481 ctatttccta tttagaaatt ttattttata acttggaaat ctatacccat attatacggc
     3541 agaaaagaaa aattgcaaac gcttttttca ttatttttga attctttgct cgatttcgct
     3601 ttttgtttgc ttgagggcac tgattatttg ttcttttgtc tcatctgtta aacgatattt
     3661 cggcatactt acactaaatg caccaatgat ttgttcgttt ttcacgattg aggctcctat
     3721 acaaaaaata tcacgttcga tttcttcatc gtcaaatgcg atgtgggttt cttttaccgt
     3781 tttgatttcc tttttcaacc gtaatgcatt ggtgatcgta tattctgtgt aagggatcaa
     3841 cggatgcttt tcaagataat cagtgtactg ctcttcactg aattctgcta atacggcttt
     3901 gcccatcgca gaattgtata atggtcgggt gattccgatt tttgaagaca tgcggatcgt
     3961 ttgatgtttt ggatctagtt tgttcacgta caagatctca ttgttgtcca atacccctaa
     4021 gtggatagtc tcatcgatct tttgctgcag ctgttctaaa aacggtaaag tcaactctac
     4081 caaatcgatt tgttcaatgt ttttattcgc gtagcgaata agctttgcac ctaaacgata
     4141 gtttttctct gaatttttat ttacgtagcc gatcatcaac aaagtatcta gtattttcaa
     4201 gactgtagaa gaggtcattc ccacaccctt tgcgatcgtc tgcaaagaga catcttggtt
     4261 ttccgctaaa tagtctagta ttttagaggc tttgatcaat acagttccat aaggcttttt
     4321 attttccata taatattctc ctatttcttt ataagaaaaa ttatagcgga aataccatca
     4381 atttaaaaga gatatccaga tataaactac catttctctt tacaggcaat ttaatgttga
     4441 agatccgctt cgtttttaca tcaaactttg atataaagca atgatttctt ctactgtcgt
     4501 ttctcttgga tttcctggtg tgcatacatc tcgcaaagca tcttctgcaa ttgctggaat
     4561 atctgcttct tttacaccta attcagaaat ggtttctggg attccaaccg ctttgctcaa
     4621 ccgatcgatc tcaccacaag ccgcttctct tacatcttct aaagacattt cagctgctcc
     4681 tttaatccct agaacaagag cgatctcacg gtacttttct ccagtatatt ctttgttata
     4741 tttcatgaca gttggcaaaa gtgctgcaca cgctacgcca tgaggaatgt tgtaccatgc
     4801 acttaacggg tgagccatcc cgtgtaccag tcctaaaccg acatttgaga agcccattcc
     4861 cgcaatatac tgtcctaatg ccattttttc tcgcccattt tgatcgccat ctacagaagc
     4921 ttccaaagaa tggccgatga tttcgattgc ttttaaatgc agcatatcag tcatttccca
     4981 agcgcctttt gtgataaacc cttcaatagc atgcgtcata gcatccatac ctgttgatgc
     5041 agccaatttt ttcggcattc ccatcatcat atcgctgtcg atgaacgcga cgattgggat
     5101 atcatgagga tcaacacaaa caaattttcg gtgatttact tcatctgtga tgacgtagtt
     5161 gatcgttact tctgctgctg ttccagaagt cgttggtata gctaatatag gtaaacatgg
     5221 atttttcgta tcagcaacac cttctaaact aatgacgtct gaaaactctg gattcgtcaa
     5281 aataatccca atggcttttg ccgtatcgat tggcgaacct ccaccgattg cgatcaaaca
     5341 gtctgcctct gcctctttaa caaaagccac tccatctttt acattttgga tcgttggatt
     5401 aggtacgatc ccgtcgtata aagcataagc gattgctgct ttatctagaa gttctgttac
     5461 ttttgttgct ataccgtgct cgatcaatcc tttgtcagta atgacagctg ctttgccgaa
     5521 tcctcgctgc tgaaattcag ggacgatcga ctcaatcgct ccttttccaa aatacgatgt
     5581 ttcatttaaa atcattcgat tactcatttt ctattcctcc taatttgtat tatttttcac
     5641 aataaaaacg aaagaacccc aagcaaccgg ttgcccagtt gcttgttgct acagttcaaa
     5701 gactggcatc aattcccaac tgactggaga ggcatctgga tttgtctcca tgatatcttc
     5761 catgtacttc caccattttt gattgataga tgtatttgcc attttttccc ataacttttc
     5821 atcttcgatc actaagtaac caaataacaa acttgtactc ggatcaagat agatcgaata
     5881 gtcgatgacc ccgtgttcac gaagtgctgt acgcatttct ggccaaagtt catcatgtct
     5941 ttttttgtat tcttcttgtt tttctgggta aactttcatg caaaaagctt ttttcaccat
     6001 gtcagacacc tgctttcgta tgaacttcct ccaaataacc tgcttttgga gtgacaccaa
     6061 acgcatctgc taaatgccac aggttttcgt ccgtgattgt ttgcaagatt ggtccttgcg
     6121 ctttgacgta cgtatagact tctgctgcct tttctgctgt ctcgatcaaa ccaaagactt
     6181 catccatgtc ttttcctgac ccgtagattc catgttgtgg ccataaaacc aatcgagttt
     6241 ctttcatttt ttcagctgtt gcttccccaa tctcatttgt tcccggaacc atccacggaa
     6301 tgatggaaat tccttctgga aaaacaacta agcattccgt acacatttgc catagcgtac
     6361 gactaaagga acgttcgtct aattcatgcg taaaactcat cgctaataga tgactagcat
     6421 ggcaatgcat aacgatccga ttatctgggt ctacttctaa acgggcgata tgactcatga
     6481 aatgacttgg caactcgctt gtaggaaggc attcgttttc cagcccccac aaaagttcca
     6541 gtgttttgcc atcttcagca actcgaacga ttccaagatt ctcggcaggt tcgctcgcta
     6601 catttttaaa atatcttcct gaaccagtta caataaaata gcatcctgct aaacggctgg
     6661 catcaaaaat catcgagatg cggcggatca ctttttcagg atccaagaac ggtgtgatct
     6721 cttcttcttt tagtaaataa gaaatattcc cgccatttct ttcatcccat cccaagcgat
     6781 aaagattcga cgtcgtttcg atcatttctt tcacataagg cgcttgtaaa atatctattt
     6841 ttttcaatat agccacactc ctattttctt aattccaaca cgtctttttg atattcctgg
     6901 acttcttcca gccaatccaa accaactgga acttgatttt tgtaacagaa ataattccat
     6961 acatcggcat acgggaaatc tttcaattct tccgtataag ccattcttga agtaaagtca
     7021 aaagccagct ccatttcttt caatcgctca actggttcca gcatagcttt cagcagtgct
     7081 ttttgtgtat ttctcgtacc gatgacccaa gcagcgatac ggttgatcgt tgcatcaaag
     7141 aaatccagac cgatatttgt tttttccaac aattcgttac ggactaattc tttagcgatt
     7201 tcctgcaatt catcatccat caaaaccaca tggtctgaat cccaacgcac tggacgggaa
     7261 acatgcaaca tgattccttt gctgaataaa gctaatgaag ataatttatt ggaaatcact
     7321 tctgttggat gaaaatgtcc agcatctaga caaacaagtt tgtcacgagt caacccgtaa
     7381 cccatataaa attcatggga accgactgta taagcttcag cgcctaaacc aaatagtttg
     7441 ctttctactg catcttgtac ataccgttca tctaactctt ctgtaaaaat ttcatcgagt
     7501 gattccatta aacgttcccg tggggtaaaa cgatcaatcg gattatcttt catcccatca
     7561 ggtacccaaa agttattgat acttgtttgc ccagtttctt tcccaaagta ctccgcgatt
     7621 ttgcggcttc ttttcccgtg ttcgatccag tattcacgaa cttctttatc tggatgagcc
     7681 aatgtaaagc cgtctttcat cattggatgc gaaaagaaag tcggattgaa gtcaagaccg
     7741 atttcttctt tttttgccca ctctacccat ttttcaaaat gtttcggctc aatttcattt
     7801 agatcgacag attcttctgt atccaaatag atagcatgaa ggttcaattt gtgtttgccc
     7861 ggtatcaacg aataagcctt ctccagatct tgtctaagct cttcaggtgt tcgtgcggct
     7921 cctggaaaat tccctgtcgc catgattcca ccggtcaatt cgccatccgg tgtcaagaaa
     7981 cctttgacat catctccttg ccagcaatgc atagaaattt tgatgtcctg tatcttttct
     8041 agtacagcat ctgtatctac accgatagtt gcatattttt cttttgcttg ttcgtaattt
     8101 ctttcaatcg tgttcatacg attactcctt tgattttatt cacgtaccat tccatcgtgt
     8161 tttgcttatt ttcatctggg taaaaagttt cacatggaaa cgactgtttg attacatttc
     8221 ttgcttgttc tatattcgaa aactgacctg tactgagcat ttgcatggca acattcccga
     8281 ttgctgtggc ttcactaggt cctgcttcta caatgattcc ggcataatct gctgtcaatt
     8341 gattcagtag acgattattc gatccgcccc ctacgatgtg cagcttttcc aatttcgttc
     8401 cagttaagtg ttctactttg atcagctctt gtgcgtaaca aagagcaaga ttatcataca
     8461 cacaacgagc gatagctccc gatgagacag ggactggctg ctctgtttca aaacagtatt
     8521 gctgaatctc ttctatcata tttgggggat tcaaaaatcg agggtcattg atttccatca
     8581 cttgctgcag cggtttttct tttgcagcaa gctctgccaa ttccgcataa ctgtactcgt
     8641 actcttgcat tcgggcaact tcctggatca accacatccc catgatgttt ttcaaaaaac
     8701 gcaccgtgtt atgggcaccc cattcattcg tataattttc agaaaaagca ttgcgagaaa
     8761 cattggcgat ctttgtttca atccctaaaa gcgaccatgt cccactgctg atataacccc
     8821 aacgttctcc ttgacctggc gttcctaata cagctgacgc cgtatcatga cttgctactg
     8881 tgataaaggt cacatctggc aaatcatatt ttgaaaattg ttctttttgc agatctccta
     8941 gtatcgttcc cggctctgtc aatggtggaa ataattcctt cgagacaccg attttttcaa
     9001 gaagttctat ttcccattct tttgtccctg cattcagcag ctgcatagta gatgcattag
     9061 ttttctccgt caccatgtta ccagtaaaga cataccctaa ataatcaggt atcagtaaca
     9121 atttatgtgc ttttgccaaa ttttcttttt cttcagcaaa caattggaac aaggtattga
     9181 aaggctggat ctgaatccct gtctgctcat acaacttatc tagagaatag atttcagaaa
     9241 actgttctac tgctccattg gtacgcttgt cccgataagc aatcggatca tttatccgat
     9301 ccccttgttc atcaatcaga cagtaatcga ctgcccatgt atcgatcccc gcaaaaaggc
     9361 gatcaatccc tgtctgcttt aatttttcaa atccaatcaa aatctctttg ataagataat
     9421 cgatcttcca gcgatcgtga ccgtctttgt tccgaaagcc gtttttgaac cggtgtactt
     9481 cttccaagat caaagaacct tttgcatccc tcgtactttt cattagtcgt ccgcttgatg
     9541 caccgatatc gatggcaaca aatgttggat tcatttctcc acctctttta ctttattgtt
     9601 gattagcact ttattaataa agcgttatca tttattgaca atgctatcat aatacagaat
     9661 cttatatttt gcttcacact attttggtat ataattgcat tatcttgaca agttgggagt
     9721 gtttacatgg attactggga aaaagccaac cactcatggt cggaagactc aaaaagagtg
     9781 atcttgacgc ctacacaaag aagcaggaat ttattttttt atatccaaga aatcggttac
     9841 ttcaaagcac caatccctta ctatactgaa cgagcacatt taccttctta cttgataaag
     9901 tttactttgt ctggcaaagg ttcgcttaca tataatgata atcagcaaca gctaaaagct
     9961 ggagacttat tttttattga ctgccaagaa tatcagcgtt atcaaacgat cagcgaagaa
    10021 ccatgggaaa tggattggat ccatttgaat ggtcagaacg tccaatcatt ttatcaggag
    10081 ttcacaaaag acggccataa caccttccat tcaacaggag atccatcaga aaacaagatc
    10141 caccatttga tcacagaatt gatccacatg caacaaggca cgaatgcccg taccgatttc
    10201 cagtcctctg tagctatcca cgagttgctg aatgaactga ttttgcaaaa gtatcagcta
    10261 gatttcgaag aagaggatat tcccgcctac gtttacaaac tgaagcaata cttagacgaa
    10321 catttcaaag aaacgattac acttgatcaa ttagaacaac aattcttatt gaataaatat
    10381 cagctgagca aagatttttc caaatttatc ggttctccac caatcgatta tttgatcagc
    10441 aaaaaacttt cttacgctaa agaccttctt cggtatacag aatttacggt tcgaatgatt
    10501 tccgaagaaa tagggattga gaattttgct tactttagcc gcctgttcaa agcaaaaaca
    10561 ggactatcgc cgagagactt tcgtaaattc agctgattca tataatatgt acgtcatttt
    10621 ttcaaaatat tttgaaatat atagataaaa ataaaataac cgcagtttct gcttctatgt
    10681 aaacaacagc agctactgcg gttatttttt agtgatcatt ctcattgttc atcttcaaaa
    10741 tgttcataat gaaccaactg tttctgtgta tcacgtttca aaaattcttt aggcgttatg
    10801 ccgaactctc tcttgaatgc tttcagaaaa tgcggataag aagaaaaacc acttttacta
    10861 tatacagcac tagagctgcg gtattctcgc aacaagtgct tggcatagat cagctttttc
    10921 ttcattacat actgatgaaa cgtaaatcca gtgtactttt taaattctcg tgtgatatgg
    10981 tatttgctga caaaaaactg cttttccatt tcctctaaag aaagcggatg agataaattt
    11041 tttgagatat attgtaacat ttgttccaca cgctcattag gaactacagc attctgttct
    11101 tgcgcactct cttctttttg tacaacttga ttgagaaaaa tcaagtattc taccagagct
    11161 tgttcatatt ttatgtcttc accgtaaatt tttttatcag gaggatggtc gataaatgat
    11221 aatttttgct tcaatacttc ctgttctatt tgcaatacac gactcctctt ctctcccatt
    11281 ggttgaaaac aagccgatag attagaccat ggcgtggaac gactttctag aaaagaaggg
    11341 gtgacaaaga tatagacacg ctcgaataca tctgaagatt ggcggacgat acgatgcaaa
    11401 tcattggaat ggatgagcag cacacttcct gctttgatcg taaattgtcg tccgttcaaa
    11461 taaaaagtcg cttctccctt taatgtagca tggatctcat agaaatcatg atgatgatag
    11521 atcgttttat tttggctgac gacatctttg acatgataga tttcatactg atcagaaacc
    11581 atcgtattga cttgttcacc gctgattttc ttttggagca tgtgccgcac ctccttttgt
    11641 tcacttatgc acaattttat tataccacgt tttttgtaat caaaaagagc tgcttttata
    11701 tactcttata aaaaagaaga gacgtggcat cttctagccc cgccccctcc attctaaata
    11761 ttagttaacc aacgtcttct ttacattatt caagaagact tcttccataa cgaatcccat
    11821 catcttttgt ttcttcaaga accacataca ggtacggacg attcttcatg atgatttctt
    11881 tgatttttgt gtactcgatc acaccgtctc ccaaatttac cggcacgatt ttttcctgtt
    11941 cgacacgaaa atctttcaaa tgaaccgcac atatcttttc accaaaacgt tcaaaagctt
    12001 cttctaccaa ttggacttgt tcttgataat cactacttgt gatcaaattg gtcggatcta
    12061 aaatgattcc tagatagtcc gaatctactg cttggatcag ctgctctacc cttgctaaag
    12121 aatacaccgg atgattcaaa cctggttcta tccccaccat catttggtat ttttctccag
    12181 cttttaccaa gcgacggata acggacacag cttcagcaaa tgcttcatct gtgaaatttt
    12241 cttccgtata ttggatttcc ggcaaaacac atccagtttc cgaagcaacc atcgacgcac
    12301 caaaggaaga agcatagcga agatactgct cgaactttgt cagcaactct tgtcgtatcg
    12361 ttaaatctgg atgaatcata ttgatatagc aacttaaaat tcctacggtc acttcttgct
    12421 tttctaaaac tcgtttgaca taatttccca tcccagaatt gatctcattt gcgccagaag
    12481 gtatttcagg aaacgaaaga cctaaggcga gctgcagtgt atgaatccct tgttcttttg
    12541 ttttttctgc aagatcttct atcgtttgga cttcggaaag atcatgtcct ctaattgtaa
    12601 tgttcaacat aactattctc ctaactcagc atattgggta gccacatgac gatttgcggg
    12661 aagtaagtaa tcaaaaacaa gacgactaag ataaccccat aaaaaggaag aagcggtttc
    12721 agcattgcct ctgcttttac gcctcctaca cttgcaccga catatagtcc tgtacctact
    12781 ggcggcgtga tggagcctac acacagattc atgattgaaa acaacccgta atgtacagga
    12841 tcaaccccta cacttgtgac gattggtaaa aatattggtg taaaaatcaa gatagcaggc
    12901 gccacatcca taaacatacc caccagtaat aacacaagat tgactaataa cagaataata
    12961 aatttattat ccgttacgcc cataatcaac gaactaattg cctgaggaat accggtaaac
    13021 gccatggcga aagacatcat ggaagaagta gccaataaga acatgatcgt ccctgacatt
    13081 gctactgatt gaaacaacat attaggtata tcttttagtt ttaccgtttt atagtaaaac
    13141 atagcaatca acagggagta aatgacagct atcgctgaag cttctactgc tgtaaatatc
    13201 cccgtcaaga taccaccgat aatgatgaca atcaatagaa ggctaggaat cgcctctttt
    13261 attatttttc cagcatgagc catctgtcct ttttcgacaa cagggtattt attcttttta
    13321 gcaacgatga atgctactag catgattcct agcgcccaca aagcgcctac gagataacct
    13381 cccataaaca aagcagagat cgaagcacca ccagcaacaa gtgaatacat gataaaagca
    13441 gtacttggag gaatgaccat acctgtcgga gctgacgcga tattgaccgc tgtcgcaaac
    13501 ttccggtcat atccttcttc cacttgctga gggatcaaca ctccgccaat cgcggtagaa
    13561 gcggcaatgg ccgagctaga gatggaacca aataaagcat tccctaacac attcgtatga
    13621 gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa aatccactag tttcttagct
    13681 atccctccat tattcatgat aatgcccgag agaacgaaaa acggaatagc gatcagcgaa
    13741 aaactattga tacttcccac cattttttga gctgaactaa aagcagaaac atcaaacggt
    13801 ataactaaca ataaagtcat catcgatgag aatgcaatac ttatagcaat cggcatccct
    13861 aatacaagca acacaacaaa tacaaaaaat aacactaatc cggcttctat tgccatttta
    13921 ttcctccatt tttatatttt ttcttgaatt gctggcttgt tgcttaatgt atcgattaga
    13981 ttgatcaggc tatagataat gatgagtacc cccgatacag gtactgacag ataaacatat
    14041 cccataggaa tacttaaaga aggcgagatc tgccccattg tcaatgtgac agcattccat
    14101 ccgccataca ccatgatcat cgcagcaaat accagaaata aactttgaac aacaacttct
    14161 aaaattttac tttttttgcc agttaatttt tcacttaaaa ccgttacggc taaatgtgct
    14221 ttttgtccca caacatacgc ggaagacaac atggcaaacc agacaagacc gaatcgaaca
    14281 agctcttccg tgaccgtatt tggatttcct aaaaccgttc tggaaaatac ttgccataaa
    14341 acaacagcga ccatcccaac aagcaaaaca gctcccagga tctctaatac cttgtttaaa
    14401 atcattttca tcacagctac gctccttgct cttggatctt ttgataaagg tctttgtagg
    14461 taggatcgtt tttgaatata tcatgaagtg gggcaactgc ctctcgaaac ggtgtattat
    14521 ctacatcatt gaattggact ccatgttctt cttgtgctaa agctttttct tcttcaattg
    14581 cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt cgctgcttct tcaatggctt
    14641 tttcctgctc atcagttagc tgttctttga tggcatcatt catgatgatg atatctggca
    14701 cacgtgtatg ctcatcatag gaatagtatt tagccacacc accatgtcca gctgtataca
    14761 agacaaattc attgttttct gatccgttga tcaaattgga ttgaagagac gtgtacactt
    14821 catcacttcc cataggtacg ggcgaaccgc ctaaaagctc taccattttg atagcggttt
    14881 cgctttgcat cacacggatc ttctttccct tcaaatcttc cggcgtatgg atcggaccgt
    14941 ctaccatata aaagctgcgc tgaccagaat cataataggt caatccggta aatcctaaat
    15001 ttttggtcga ttggtagatt ggctccatta tttcttcatt atccatcacc ttaaagaaat
    15061 gttcttcatc attgaataga taaggaactg aaaaaatcga ataatcttta gagaaacttt
    15121 ccaacgctga ggcacttact tttgtgaaat ctactgctcc tgactgtgtc aattcgatca
    15181 gttcacgttc tccacccagt tgtccatctg ggaaatactg gatctgtacg gaaccatctg
    15241 ttttttcttt tactaaacgc ccaaattcat tcattgcatc aattactggc tggctgttgc
    15301 ttgcataagc aaagcgcaag gtaactatct catttacatc tgcatctgcg acagttgcca
    15361 cactagaatt atttccgcag ccgcttagac ctagcagacc taggctaagc gaacttgctg
    15421 caaacagcag tgatgttttt ttcatatggg tttcctttct tttaacaata agagacgagc
    15481 aattctgtca aacaaagcac cgttaacgac tgtccataag gcattgctgt gattccgata
    15541 ttccgataaa aatctaaact gtcccccatt cctgtcccga ttgaaacgtt ttgaacttct
    15601 cctttttcat cgatttgttc aagcaatcct tggatagcac gataagcgac ttctttatat
    15661 tcttgtggta aataacgttt atggaccgct ttcaaaatac cgtaagcaaa tcctgcagtt
    15721 gcagaagatt ctacgtaaga agaaggatca tccaacaacg tatgccataa accgctttca
    15781 gactgataag tttttaatgc acgcacttgt gcttcaagtg tttcgatcaa taatttgcgc
    15841 aatgcatctt ctttcgataa ttctaagatt tcaatgatct ctggaatagc aatcgtcaac
    15901 cagcagtttc cacgagccca aaatgcttcg gcatagttat gatttccttc aaacgtccag
    15961 ccgtgatacc acaatcctgt tcgtttatcc atcaagtatt tgatatggat cataaactgg
    16021 taacgagctt cttctaaata ttctgggcga ttcaacagtt tcccgatttt tgccaaagga
    16081 agcactgtca tcatcaatgt atcgtcccac aactgattct tattttccgg tccatatgtg
    16141 gcatgctgta acccgtcttc atttgtccga ggcatgtcat tcatgaccca ttctgcccat
    16201 tgttctaaat aaggcaaata gcgtgaatct ttcgtgtctt catacaagta agccatagtc
    16261 aatagcggtg ccattgtatt gacgtttttg ggcggagcgc cttcttccat acgatcctca
    16321 aaccattcat tgaccacttg ataagcgcga ggattttttg tcatctggta atttttataa
    16381 ataccatata gaccgacacc ttgtggccaa ttccacactg tccagctttt gtcatctact
    16441 ttcagtccat caaagtccaa caaaaattca ccattatcgt ctttgatcgt tgtcaggttt
    16501 tcaacgagca ggtcaatttt ttctttcact aattctttag gtacaaaaat ctcttcttta
    16561 ataatcatct ttacgccacc tttttaaacg ctttcatttt atatccttaa tatagcagta
    16621 ataccgtcca ctataaatat cgctttttga acaaaaactg tctaaaattg acctaaatgt
    16681 taaataacaa aagtaacttt cagttacttg gaaattaaaa aaagaacttg gagaaacaac
    16741 gttttccaag ttctttttca tctattttct tatctttttt ctacgctttc ttttgcttat
    16801 ccgttgtaag catcaatgat gatctgtttc aattcagaga tcaatggttc tttcggatta
    16861 gctgtcgtac attggtcttc gtaagctaat tcagccatac ggtctacggt tgtatctagt
    16921 gtttcttgtg tgactccttg agcttttaga ctcatgttga tgccgacaga ttttcctaat
    16981 tcataaacag ccgttgctaa agcttcgact aattcctctg ttgtttcgcc tttcagacct
    17041 aagaattttg cgatatctgc gtaatctgta tctgcacgga aatagtcata tttagggaac
    17101 atcgcatgtt tttgtggatc ttttgcatta tagcggataa tatgcggtaa aagaatcgca
    17161 tttgtccgtc catgagggat cccgtattcc ccgccgattt tatgtgcgac tgagtgacaa
    17221 atacctagga atgcgttagc aaacgccata ccagccattg tagaagcgtt gtgcattttt
    17281 tcacgtgatt ccatatcagg tgtttttact gatttttcta aatagtcaaa gactaacttg
    17341 attgcttgta ggctcaatcc acgtgtataa tctgaagcca tgactgaaac ataggattcg
    17401 attgcatgtg ttaacacatc catcccggta tctgctgtga cagatgctgg aactgacatg
    17461 acgaattgag gatcgacgat tgcgacatct ggcgtcaatg cataatctgc taacggatat
    17521 ttcacatgtg tttcactgtc agtgatgacc gcaaatggcg ttacttcaga accagtccct
    17581 gatgttgtcg ggatacatac gaattgtgtt ttttctggtt tgtcgatctt atatgtccgt
    17641 ttacggatat ccaagaattt ttgttttgca ccaaagaaag atgtgtctgg gtgttcatag
    17701 aacatccaca ttcccttagc tgcgtccatt gcagaaccgc ctccaagagc gattactgta
    17761 tctggcttga agtctgccat cagtttcgtt cctgcgtata cggtatttgt cgatgggttc
    17821 ggttcaacat ctgagaagac ttcgatcttc acatcatttt tacgtcgtgc caatacttca
    17881 cgaactgtat cgcagtaacc gaattggacc ataccagggt cacaaacgat catgacacgt
    17941 tcaacgtttt ccattttctc taagtacaat agagagttct tttcaaagaa gatttttggc
    18001 ggtaatttga accattgcat gttatttctc cgtttcgcta cagttttgac attgattaag
    18061 tttacagcag agacgttacg agaaacagag ttcttaccat aagaaccgca tcccaaagtc
    18121 aatgacggaa tcatttcgtt atagatatca ccgatgccgc cttctgcaga tggtgtattc
    18181 actaaaatac gacaagcttt cattcgaaga ccgaatttga catgcaactc ttcgttttct
    18241 gaatggataa ctgctgtatg tcctaatcct ccaagattca acataccttc acacaaatca
    18301 aacgcatggt ccgtgttgtt agctttgatc atggcaagaa ctggagaaag tttttctctt
    18361 gatagcggat aatctgggcc tacgccttcc aattcagcaa tcagcatttt cgttcctttt
    18421 ggtacactga ttcctgccaa ttttgcgatt tccatcgcag aatgtccaac gattgatgga
    18481 ttgaccgcat attttccttc gttcatcact gcatcttcta atttttgcaa ttcatctggt
    18541 tttacgatat aaacttggtg tgcttggaat tctgctttta ctgctgcata gatttcttta
    18601 tccacgatca cagcttgttc agaagcacaa atcattccat tatcaaatgt tttcgaaacg
    18661 atcaaatcgt tcactgcacg tttaattttt gctgtttttt caatataagc tggtacgtta
    18721 cctggaccta cacctaaagc tggttttcct gtagagtaag cagacttgac cattccagct
    18781 ccacctgtcg ctaagacgat cgcgatacct ggatggttca ttagcataga agtcgcttcg
    18841 atcgaaggat gttcgatcca ttggatacaa ttttcaggag ctccggcagc gatagcagca
    18901 tcacgtacca ctcgtgctgc ttctgcagaa gatttttggg cacttggatg gaaagcaaaa
    18961 acgattggat ttcttgtttt caacgcgatc attgatttga aaatcgttgt ggaagtcgga
    19021 ttcgttgttg gagttactcc gcaaaccaca ccgactggtt cagcaatttc aatcaaccct
    19081 ttttggacat cttcattgat cacaccaact gttttatcat gtttgatatt atgccaaata
    19141 tattcggacg catacatatt tttgattgct ttatcttcgt aaacaccgcg gcctgtttcc
    19201 tctacagcca tcttcgctaa tggcatatgt tgatccaaag ctgccattgc catttcatgc
    19261 acgatatggt ctactttttc ttgatcaaag ctttccattt ctttcaatgc tgcatttgct
    19321 ttttctgcca aatcatcaat cattgcttgt acgtcaattg tcttcgtttt ttctttttct
    19381 attactttag ccatcattat cctcctaatg ggtcatggtt atttgttaaa taattcacat
    19441 gtatatcata caatatttat ttttctatgt aaacactttt ttgtgatttt tttcacagtt
    19501 atttttaaat ataaaattgc tttatttaca ggtgtttgta tagaaaaata tataaagtta
    19561 tttattatta tttatatgac acacccgatt attcaatatt gagcaatata tcccatgctc
    19621 ttatctcttt cttttttgaa taaaaaatga ggtaggacat ctttctgccc tacctcgtct
    19681 ttaaataagc tttgatactt atttttcttc cttctcttct gtctcttcca gcgtttctgt
    19741 tgtttcagtt tttacttcaa ctgtctcagc tggttcttct gttgtttcaa cagtagtcgt
    19801 tgcatcattt gttacgacag tccctggttt gaccgttttg attgctgcac gatcgtactc
    19861 aaggaagatc ccttcgcagt ctaaaacaac tgtccgtttg tcattgtcga tttctgaaac
    19921 aactccatgt aatccgccga tcgttacgac tgaatcacct ggtttcatgc tatctaacaa
    19981 gttttgacgt tcttgctgtt gtttcttttg cgaacgtgac ataaaaaacc acattgctaa
    20041 tagcgcgata aacatcacaa gcattggtaa tgttcccatt tttttcacct cactaattct
    20101 gtatctacaa aaaacacttt agcagaaaaa tgctgataac actagttttt tacttaattt
    20161 ttaaaagatt ttcctttaga agctttttgc gttttcttta ttgaacccgt attcttcaaa
    20221 aaaggcttct ctaaattcaa gcaaattatc atccatgatt gcttgacgta cttgtttcat
    20281 tagattcaac aagaagtata aattgtggta agaagtcaaa cgaatcccaa atgtttcatc
    20341 acatttgatc aaatggcgga tataggcacg tgtatagttt ttacatgtat aacaatcaca
    20401 tttctcatca attggacgga aatcatgggc atatttcgca tttttcacta ctagacggcc
    20461 ttgagaagtc atacatgtgc catttcgagc gattcgagta ggcaagacac agtcaaacat
    20521 gtctactcca cggataacac catcgatcaa tgaatcagca gcaccgacac ccatcaagta
    20581 acgtggtttg ttttcgggga tcaacggtgt agtgaattct aatacacgat tcatttctga
    20641 tttaggttcc ccgacagata acccgccgat tgagtaacca gggaaatcca tcccgactag
    20701 atctttcgcg ctttgacgac gaagatcttc aaaccctgct ccttggatga ttccaaataa
    20761 tccttgacgg tctggattag catgagcttt caatcctcgt tctgcccatc gagacgtacg
    20821 ttctactgat tttttcacgt agtcgtaact ttcatcgaat ggtggacatt cgtcaaaact
    20881 catcatgata tcagagccca atttattttg gatgttgatt gctttttctg gtgataaaaa
    20941 catacgcgaa ccgttcaagt gatttttgaa atgcacgcct tcttcttcga tattccgcat
    21001 atcacttaat gaaaaaactt gaaagccgcc tgaatctgtt aaaatcggtt gatcccagtt
    21061 cataaattta tgcagtccgc cagcttcttc tactaagtct tcaccagggc gtagccataa
    21121 atgatatgta ttacttaaaa taacgccagc acccatttct ttcaactctt ctggggacat
    21181 tgtttttact gtagctaatg ttcctactgg catgaacatt ggtgtgggga aagttccgtg
    21241 tggcgtgatc aattccccta aacgcgcgcc agtatgtttt tctttcttga ttaaacgata
    21301 gcgaatggca ggttgcgtca ttcatttccc tactttctat ataaaatatc ttgacaactc
    21361 ccaccatcat aatcgtttca agtgcttttt gcaagttccc tcggcttcct tctactttat
    21421 ttaatattct tttttttatc ctttttttat gaataacaaa aaaaagaaaa acattatgac
    21481 agaaatattt tttgatacat ggtatattgg tgttattaaa gaaaaggagg gaaaaatcat
    21541 gcaatcttct gcagaattaa aacgccaagc aaaagagagt ctaaaaggac gttggggaca
    21601 agctgtttta ctaaatctaa tccctacatt gattggtatt gctactattc ttcttattat
    21661 gattcctatt atcttgtttg ccaccaccat atatgatgga tcagcgagcg tctcagaagc
    21721 gacaacttca ggaggcggta gttcaggagg cggtggcgga attatttcca gcatcattag
    21781 cgctttgttc atgagcggta tttcttggac ttatcttgat attttacgtg gacaaaaaga
    21841 tcagatcgaa ccattcaaag atgcatttcg tggattcaaa ggtttgttta tcggcggagt
    21901 gatcagctta gctgtgctga tctcgatctt tacaacatta tggggtttgc tattagttat
    21961 tcctggaatc attaaaagct attcttattc tcaaagctat tttatttact acgatatcgt
    22021 ccaagaaaca ggacaaacac ctaaagtact ggatacgatc actgcaagtc gaaaattgat
    22081 ggatggttat aaaggacaac ttttctggtt agatctttca tttatcggct ggcatatctt
    22141 agcattagca actgctggta ttggttatct atggctcagc ccatacattt ctgcaacaaa
    22201 agcagcattt tatgaacaat tgccaaaaga tatttaaaag agagtgtgac ttgtgaccga
    22261 gggggtggca ggagaacacc gtttatcgag aggcagtaaa agaaacattc agcccattag
    22321 caaaaaggag gtgtgacaac attcatgtca cacctccttt ttgtatagtt ccagtaaaaa
    22381 gaaaatcaac ttagaaacag ttaatcattc ctcttcttct gcttccggta atcgatggaa
    22441 cttgatcagt tcattcaccg cgattggtac gatagataaa atccctacca agaaccagtg
    22501 attcaaactg atgtgtacta ggccgaatag ttcttgcgta aatggcagca aagcaacagc
    22561 cgttgtaata gctaatgcca gtaacgccat ttctaccaaa gctttattgg tcgctaaatt
    22621 gactttgaag atcgattggc tgctgcgtac attgaacaca tgcagaatcg atgaataagc
    22681 taagatcaag aatgcaacag tttgaccgac atgctgactt gcgtctaccc atgggctgat
    22741 agtatcgaca taactaccta aataataacc aaacaaggtt acgatggtga agacccctgc
    22801 atttatggca attttttgca ataaacctcg agcaaagatc ccttcatttt tcggaatggg
    22861 tggctgatcc atgatgccgt gttccgctgg ctctcttcct aaagcaaatc ctggaagtcc
    22921 atcagcaact acattgacaa acagtagttg cacagctgta aatggtgctc cccatcctaa
    22981 cagcatcgca atcaaaacaa taaagatctc cgaaatatta cagcttagca aaaagttgat
    23041 agctttacga atattctgat aaaccgaacg tccttgactg acagcgtcta cgatcgtcgc
    23101 aaagttgtca tccgtcaaaa tcatatctgc ggcactttgc gccacatctg ttcctgtgat
    23161 ccccatggca cacccaacgt tacttgcttt caacgctggt gcatcattca caccatcccc
    23221 agtcatcgct acgactgcac ccgtacgttg ccaagatttg acgatccgga tcttgtcttc
    23281 tggcgttacc cgagcataga cagagagagc tttgacacgc gcatctaatt cttcgtctga
    23341 taattgatgc agttctgatc ctgaaagtgc ttctgacgga tcattcaaga tacctaattc
    23401 tttcgctatg gcactggcag tcaccacatg atctccagtg atcatgactg ttttgatgcc
    23461 agcctttttc gctcgcttga ttgctccttt actttctgga cgtggtggat cgatcattcc
    23521 gatcagacca atcaaacgta agtctttttc cagtgcttca gaagaaatat cttgcggttc
    23581 ttcgtcataa gtagcataac caacagcaat cactctcagt gcccgtttac caaagcgatc
    23641 attaacgatc gccgcttgtt ctacatcgcc tgaactaaag cgcggagcaa gaacatcgaa
    23701 tgctcctttg gtgattgaga tgtatttttc gcccatttgg tgaactgttg tcatcatttt
    23761 tcgttcagaa tcaaaaggaa gctcgtttac tcgtggatat ttttcttcta attctgcctt
    23821 tgtatgataa ttctcttcaa cagcccttac gatggctact tctgttggat tacctttgat
    23881 aacaaggtcg tcatcttctt gctcgaccgt cacatctgta catagtgcag ccattttcaa
    23941 tatttccatc gcgctgttcg tcattgaatc ttcgatattc gttacttcat cttctttgct
    24001 ccatacccgg cggacgcgca tttcattttg tgtcagcgtt cctgttttgt ctgagcagat
    24061 aacattagcg gttcctagcg tttcaactgc tggcaactgt cggatgatcg catgtttcct
    24121 cgccatcttc tgcacaccgt atgcaagtgt caacgtaaca ataacagtca aggtttccgg
    24181 tacagctgca acagccaagg aaatcgaagt catgaacatc tcaaggagtg gttctccttg
    24241 cagttcacca atcacaaata ctaaagccgc agccgctaaa gcgatcatac tgattcgttt
    24301 gcctagctga ttcaatcgga cctgtaaagg tgttttttgc atagtatttt cgttcaaaag
    24361 atccgcgatc ttacccattt ctgtagccat gccaatggca gtgacgatcg ctttcccacg
    24421 acctgcagca accgtacatc ctttaaatac catattaagt tggtcaccta atgactcatc
    24481 ttcttcagca ataaatgctg catctttttc aactgcttca ctttctccag tcaacgccga
    24541 ttcttctaca cgtaattgtg atgcttcgat caaacgggca tcggcagaaa tagcatcacc
    24601 ggactctaaa acaagaatat cacccactac cagttgctct gactcaacag tggtcacttt
    24661 gccgtctcgt atgacagttg ccgtttgttt attcatatct tgcaaggatt ccagtgcttt
    24721 ttctgcgttt ccttcttgga caatcgccaa taccgagttg atcacaacga tagcgatgat
    24781 cagcagtcct tcaaataaat ccccatgttc tgtagcaaat gcggtataaa aggaaatcac
    24841 cgctgctaca agaaggatga tcgttgtaaa atcggacaag cttcgaaaga attttttgat
    24901 catcgattct ttcggcgcct catcgaattt attcaatccc tgcttttttc gccgttcatc
    24961 cacttcttgt gaagacaacc cagtatctgg atctgcttct gttaccttct ttacttcttc
    25021 tattgtctgc ttgtattctt ccatcctatt cctcctttaa aatcttcgta caaaaaaagg
    25081 tacttatttt actataacat ccttttttta tttacttcaa ataaagggct tatcgattat
    25141 ttgttaagaa agcgtaaaat ttaaagtgct tatatttgca aacgctacca aaggatgcta
    25201 ttatatagat gtaaggaagg aggataccat caaatagaca gaagaatttc tcgatagaac
    25261 agtgtaagca aacaaagttg ttagaagtac aggtttcacc cagacatgcg tgtagagtac
    25321 aaacctataa atagtgaatg cgtatggatg tttggatcac gaagtatccg aaacaagaag
    25381 taatgcgata cgtaacaatt gaataacgta aacgtctgaa aattgaatag gaacaagaga
    25441 tgtttcaaat gttgaagatg taacgaaaac aaaatttttg tttaacatga agaaattcaa
    25501 caccgtgctt tgcatgtatg atggaaagtt acaatcattt ctgtttattt gaatccttcc
    25561 gaggtacaag aaagccgagc ttatcgagag ctcggctttt tctttgtcat aaaatctttg
    25621 taaagctttt ttgttattca aaactagcgg cttttcataa ccttatttca cgaacatcgc
    25681 atctccaaaa ctaaagaaac gatagcgctc ttcaatcgca tgctggtaag cagataaagt
    25741 caattctctt cctgcaaagg cactaactaa catgactaaa gtcgattttg gcaaatggaa
    25801 attagttgag aaagcttcta ccactttgaa ttcatagcca ggcgtaatga aaatatcagt
    25861 ccatccgctg tctgctttga tttctccatt aaatttcgtt ccaatcgttt ctaatgttcg
    25921 aattgaagtt gttccgacag ccacgatttt cccgccattt ttacgtactt ggttcaatcg
    25981 ctcagccgct tcttccgtca aacggtagaa ttcactatgc atatggtgtt cttcaatttt
    26041 atctacgctg acaggtcgga atgtacctaa ccctacatgt agtgtcagat agactaattc
    26101 aacacccttt gcttcgatct catcaagtaa ttctttcgta aaatgcaatc cagctgttgg
    26161 cgctgcagca gaaccatttt ctttcgcata gacagtttga tagcgatctg ggtcttccag
    26221 acgttctttg atatatggag gcaatggcat ttctcccaat gattcaagaa tctctagaaa
    26281 aattccttca tactcaaagt caatgatccg tccgccgtgt tccaattctt ctttcactac
    26341 tgcttgcaat ctgccatcac caaaagagat tcttgtccca actttggcac gtttcgcagg
    26401 tttgatcaat gtctcccacg tatctccttg tgtattcgtt agaagaagga cttctaaatg
    26461 cgcaccagta tctggttttt ctccatataa acgagcaggc agaactcgtg tattattcat
    26521 aactagcgca tcaccaggct gtaactcttc aatgatttca tggaaatgtt tatcttccat
    26581 ctctccagtt ttccgatcca aaacaagtaa acgagaatga tcgcggtctt ttaatggtgt
    26641 ttgtgcgatt aattcttccg gcaagtcaaa atcaaaatct tctgttgtta acatatcgtc
    26701 actcactttc tatcatgtgc ttcatcattc tatcatgttt ttcgttctgt tggcaaaatc
    26761 agcacttttt cacttctatt ttttgttttc ctaaataaaa aaactaccct atttgtattt
    26821 tttcattgtt ttaggcatac tagtaataga tagttcttca ttgaaaatag aaaaagaggt
    26881 ggcaccatga ttgaatttca aaatgtgtca aaaatttata aaggtggaaa agtcgctgta
    26941 gaggacgtca atctttcatt tgaaaagggt gagttcattt gttttatcgg tacgagcgga
    27001 agcggaaaaa cgacgacgat gcggatgatc aaccgaatga ctgatccaac acaagggaaa
    27061 attttgattg atggaaaatc catccaagaa attgatccag tggaattgcg acgtcagatc
    27121 ggctatgtta tccaaagtat tggattgatg cctcacatga cgatccgtga aaatatcact
    27181 cttgtccaaa aattgttgaa agtcagtcaa gaagaacgga ataaaacagc tgaaaaaatg
    27241 attgatcttg tggaattgcc ccgtgagatg ttagatcgct acccacatga attatctggt
    27301 ggtcagcagc agcgtattgg agtcgttcgt gcccttgcag cgaatcaaga tatcatcttg
    27361 atggacgagc cattcggtgc gcttgatcca atcactcgtg attccttgca agaccttgtc
    27421 aaagatttac aagagcgttt gggaaaaacg atcgtatttg taacccatga catggatgaa
    27481 gcgattaaac tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat
    27541 acaccagaaa atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa
    27601 gatcgcctac tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca
    27661 gcaatcacga ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa
    27721 cgtgtagata cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta
    27781 gaaacgttga ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat
    27841 gttttttacg taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc
    27901 gggctgaaat acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt
    27961 gcatcattag tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat
    28021 gttgcagagt cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt
    28081 catcagcacg gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt
    28141 gctttgatca ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca
    28201 aaagctgcca cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct
    28261 ttattggcat tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc
    28321 tttatttatt cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat
    28381 tggaattatc gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc
    28441 gttgaattgc ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac
    28501 gtgattgcat gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt
    28561 tttagtggac tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca
    28621 attttagcac tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc
    28681 gcacttagag aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct
    28741 ctcttcttat acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca
    28801 ccatttcgat cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg
    28861 gaatggttga acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa
    28921 ctatcaacca tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag
    28981 gaacagattt aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg
    29041 ctaccgtgaa agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatagtt
    29101 ttgaaaatac gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta
    29161 aagtcagcga tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga
    29221 tcaatcgtaa aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt
    29281 cgatattacc tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata
    29341 ttgttcttgg ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag
    29401 atgatcgtca attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag
    29461 ctgatacgcc tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa
    29521 aaatgcagga attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac
    29581 aacgtttctt acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa
    29641 tgtctttcaa caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt
    29701 tatccgtcac ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc
    29761 ggttgggatc atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa
    29821 tatcatccag acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct
    29881 gggagtcaat gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa
    29941 tacgtatacc gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg
    30001 gatgactgct agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat
    30061 ggcaggcata agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt
    30121 cggtgctggt ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc
    30181 gattatttta gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt
    30241 aggtattttg gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa
    30301 aaaacaaaag cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt
    30361 taaggcaata atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc
    30421 tatttatcca ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac
    30481 cgactaagtt tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt
    30541 catggaattc ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat
    30601 caattcttac atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt
    30661 tcgatgcctt cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc
    30721 ttttatacaa tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc
    30781 agcttctcgc catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc
    30841 atccgtcgca actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa
    30901 ccaattgatc gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc
    30961 gtgatgcatt gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt
    31021 ccgtttgagt gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa
    31081 cgacactggt attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct
    31141 ctgggaacca ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg
    31201 ctgtttctcg ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg
    31261 tcgttgccac tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac
    31321 ttcttcctcc ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac
    31381 ccttcaaagg tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt
    31441 tggttttaga acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa
    31501 aggttggctc ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt
    31561 attttcctca tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct
    31621 gccttcatgt gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt
    31681 caatgtctga atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa
    31741 atccactttc gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa
    31801 tcaatatcat cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct
    31861 aaagtacctg tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca
    31921 atcaatacag cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca
    31981 tttacctctt tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta
    32041 ttagccattg tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc
    32101 aaaaaaatac aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa
    32161 aagacccctt tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc
    32221 ggtagccaaa cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa
    32281 agcctgtgag tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg
    32341 attgatatct tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact
    32401 gattttggcc gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca
    32461 ttcatgttca ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta
    32521 aaaatggcac tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact
    32581 tatgaagaca catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc
    32641 tgtacattat ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt
    32701 tacagctaaa taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat
    32761 agagggcacc tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta
    32821 gagggtctgt ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct
    32881 gacaacgata ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg
    32941 gagaatattc tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata
    33001 gttttgtttc gctgcatttt tacttacgat atctacataa cggataattg gattttcaat
    33061 cgagcgataa gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc
    33121 tgcttcgatt tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac
    33181 ataatcaccg atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc
    33241 gactaataca agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg
    33301 aagctgttct gctacttttt tataatgatc atggatctta taaaaaatga acataacgat
    33361 tggcataatg atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat
    33421 gattgcatat gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt
    33481 tccttctttt ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac
    33541 gccgatagag tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac
    33601 gatcgaacct agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc
    33661 ttgatacatg tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc
    33721 agaaaaaccg gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta
    33781 atacaaaata ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt
    33841 ttcaggaacg atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa
    33901 gatcaatgcc atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa
    33961 tggtacagca ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc
    34021 tcgtaaaatc aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg
    34081 caatggcaca gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac
    34141 aaggataaat gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag
    34201 attcagcatc atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag
    34261 tgctggcacc gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa
    34321 catgtagtcg atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc
    34381 actgctgacg acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga
    34441 tagcgttaat gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat
    34501 ggctgcagca gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga
    34561 tgataacgca tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc
    34621 attttcagca gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta
    34681 aactccttta cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga
    34741 tgttatagta cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa
    34801 aacagaaaaa tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt
    34861 ttatgggctt tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag
    34921 ttctatcctt tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat
    34981 cattcttact actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa
    35041 agcttgcatc tgcggtaata agcccaacca aattaaaaac ataagaagct gtttttattt
    35101 ggtcgttctt attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc
    35161 aatcagctga ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc
    35221 atttttttct ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa
    35281 aatatggctc tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata
    35341 gattgccgtt cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt
    35401 cgtagcattt ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt
    35461 aaatggcaag aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt
    35521 cccgcctccg ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt
    35581 atcgaataat gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc
    35641 aaatgacacg ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa
    35701 tgtaacgatc aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa
    35761 ttgttctcgt ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc
    35821 aacacttgaa agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct
    35881 agctgaaccg ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt
    35941 aaagaagtca ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc
    36001 gacttcttgt tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt
    36061 tggccagtca ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat
    36121 tttttcattt actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt
    36181 ttggataaaa tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta
    36241 taggtctgcc ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt
    36301 attggcatca tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat
    36361 tgccactgca ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg
    36421 gtccgctttg ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag
    36481 agaagtgaca gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt
    36541 attaacaacg gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg
    36601 aacagctgtg ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg
    36661 atttagctga tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt
    36721 caggaactcg accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc
    36781 gttcaataca ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc
    36841 tgttcttaca gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa
    36901 tgtaacatct acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga
    36961 ttttgtttgg tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat
    37021 atcttgtact tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat
    37081 tgtctcttgg atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg
    37141 gatctctggg attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg
    37201 cattttgtca ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc
    37261 cacgaattca tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc
    37321 gaggacttgt tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat
    37381 atctttaact ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa
    37441 tactttcaat aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat
    37501 cgaacttgct cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt
    37561 atattcgatt tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa
    37621 atcctttggc aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg
    37681 ttcttttgaa tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac
    37741 ttgctcccca atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac
    37801 agcaattggc agctctccag tatttccata tggatcccat aatgctttga tgttaaacca
    37861 cgcatatagc gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt
    37921 gaaaatccgt ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt
    37981 tctccccctt cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa
    38041 tcggcttttt tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat
    38101 ctcttacata ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg
    38161 gaaagataat tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac
    38221 attttttgtc tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt
    38281 tcttattttt cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga
    38341 tgtgcttgga tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag
    38401 gctccttttt ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt
    38461 cttggaacaa acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg
    38521 tcatttaatg caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg
    38581 atacttcctt tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga
    38641 tttgttttat cataagcagc aaatgctgaa gcttctaatt tttttacctt ttcctcattt
    38701 ttactggatg cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt
    38761 gacccgaccg cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc
    38821 aaatggttga ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc
    38881 caactcattt tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg
    38941 taagtcccgc cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct
    39001 gaaccaatct ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat
    39061 gttcgtatct ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat
    39121 gcttttattt ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct
    39181 tcaaaaacat cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc
    39241 tccctttttc tttaagttta cgtttatgcc tttattttga caaaaaaaat gtcttttcgt
    39301 tttggcgcat gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt
    39361 atctaaaagc gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga
    39421 tggctaaaaa atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg
    39481 ctgctttacg ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt
    39541 tagatgctcg tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat
    39601 acatgcgtaa attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga
    39661 ttccaggagt aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg
    39721 gcgcatggac cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg
    39781 ctggtactag aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa
    39841 ggctgcatgg ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac
    39901 actaattttc aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt
    39961 ttctttcaat cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct
    40021 ttctattcct gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat
    40081 ttttgccgtt atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg
    40141 aataaccaaa actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc
    40201 aaaataaata tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta
    40261 tccgctgtcc taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat
    40321 cctgcatagt gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag
    40381 gaaattcttg aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac
    40441 agtagttccc ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat
    40501 aaccgatcgg tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc
    40561 cgattccttc acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg
    40621 ctggctgcaa tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg
    40681 ctactccata acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat
    40741 tgctgacatg tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt
    40801 ctttttgcag attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata
    40861 aaccttccca ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt
    40921 caggtgtaca aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag
    40981 gcgtctctag cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac
    41041 ctgcagttac tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat
    41101 tcaagataag gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat
    41161 ttgaaacaca aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc
    41221 catgaccgta tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg
    41281 caacttcatt tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc
    41341 ggtgccaagc gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa
    41401 aagctgtttc atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg
    41461 tcacaacagg ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc
    41521 cgatcccagt tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa
    41581 attctacttg gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga
    41641 ttcgtgcaat gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg
    41701 catcgatccc aattcctttt atcatatgct tttctctttt caataaattt agaacactct
    41761 cattctacca aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag
    41821 tttctcttaa aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa
    41881 atccgcgttt tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc
    41941 tataaccgcc ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt
    42001 ttccgccacc acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt
    42061 ctggtgtgat cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa
    42121 gtgcaactaa atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt
    42181 tatctagacc attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac
    42241 ctttgaatgc ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt
    42301 tttcaatcac atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc
    42361 cttttccggc acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga
    42421 tatcatagtt gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg
    42481 tcgctacaag gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt
    42541 gttgagacaa atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg
    42601 ccaattcgtc tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa
    42661 catcaaacaa gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt
    42721 gatcgatcaa gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca
    42781 tgaatttaac gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt
    42841 gtctttgatc aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca
    42901 acatttcatc tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc
    42961 ggttaatgtg gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca
    43021 agccacggat ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt
    43081 tgtcgcgccc taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg
    43141 cgataactaa accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc
    43201 caaatgcagc tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta
    43261 atgctagtgg aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga
    43321 ctgatttcaa caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat
    43381 atggttgtta actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg
    43441 tattgtttct ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac
    43501 caccgaaaaa tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc
    43561 tttatcatta ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat
    43621 ttctcttcat ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta
    43681 cagattttta tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc
    43741 tgccttttag cataatacta tctgttgctt caagattttg tttgatagca tcaatcaatt
    43801 tattcttttc tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt
    43861 cttttagtgc atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga
    43921 tatgttcact catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg
    43981 ctaacacagc gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc
    44041 ccatggctgt tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg
    44101 ttagccattc ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag
    44161 gtatggacag tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat
    44221 aacttccagg cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt
    44281 ttccttttgc tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg
    44341 tttgtactaa tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta
    44401 atccgtctga tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg
    44461 acccaagatt ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag
    44521 ccataagtga aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct
    44581 tttctgtatc ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat
    44641 ttccttgcgt cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg
    44701 tggtggtttt accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt
    44761 aataggttgc caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat
    44821 tttttggagc ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg
    44881 cttgctggat aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt
    44941 cggttatctt ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc
    45001 tgcccacggc ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag
    45061 gttgtgaaaa aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa
    45121 tttctctatt cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt
    45181 ctttatttct ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat
    45241 tcctaactgg atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa
    45301 gagtgggtac atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa
    45361 ttcattttta tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt
    45421 gtaagctaac ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg
    45481 aatctgcatt tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc
    45541 ttttacgact tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc
    45601 gatttcgcgc gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt
    45661 tgctaaagca ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc
    45721 catattcgcc ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac
    45781 ttttttagga ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac
    45841 accagcagct tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa
    45901 tacgcctgcg ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc
    45961 ttccccgttt ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc
    46021 cggattgatc acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag
    46081 atgcaagaca tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc
    46141 ttcttttgta ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac
    46201 tgaaaatgcg gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt
    46261 aatcttcaaa agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt
    46321 agcacaaaac aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag
    46381 aacgagcgtc atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta
    46441 tgataaagaa aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa
    46501 atcattcgag tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc
    46561 gaacaggtaa aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg
    46621 ctttctgttc gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa
    46681 gatagtgtac ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct
    46741 ctttcattat cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg
    46801 gggcaggctc atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac
    46861 cctaacatcg ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg
    46921 atcaaataca ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa
    46981 cgttccttga tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat
    47041 caaaagaaca atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg
    47101 aaaaaagtga tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc
    47161 gaaaacaaaa ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt
    47221 gcaaagcttt tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct
    47281 gatcctcatc cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct
    47341 agccgaaaaa ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc
    47401 acttctgcag aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg
    47461 gggcatcttt ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg
    47521 aaagatgctt atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt
    47581 gatgaaagtc ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct
    47641 attgatgaaa tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat
    47701 aatctgcagt tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg
    47761 gtgatccgcg tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa
    47821 cattattttc tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta
    47881 ttggaaggga tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg
    47941 tggacttcgg gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc
    48001 aggatcgaaa gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta
    48061 gggtcctccc tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta
    48121 gggatattta cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg
    48181 atccaaatgt ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc
    48241 aagaaataag aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta
    48301 tttttcgaag aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc
    48361 acccagtaat aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt
    48421 ccctttttga tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact
    48481 tggtaaacct tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc
    48541 cgtaaagggt tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata
    48601 acgttgttct agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa
    48661 cactcgcaca cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa
    48721 gtcattcttc ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac
    48781 gtagacgatt tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac
    48841 gataaccacg tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga
    48901 accatctcca tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag
    48961 ttactaatcc gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta
    49021 cactttctat cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc
    49081 ccatttcttt tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc
    49141 ctttattttg agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag
    49201 tttgataata ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa
    49261 agacacctat cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa
    49321 gcaattgcat agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt
    49381 ttgctgcaat caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc
    49441 cgtacagtat gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat
    49501 ttagaaaagt aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta
    49561 aaacaaacac gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc
    49621 tttcatcgaa taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa
    49681 ataaaacaag ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt
    49741 aacgcggata atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa
    49801 agcttgctgc aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc
    49861 agatagctgc aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt
    49921 gaaaattgac cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac
    49981 ttgaaaaata aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac
    50041 caccaatcag cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt
    50101 tgactaaaaa tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca
    50161 gccaattatg cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt
    50221 tacttaagat aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga
    50281 acatgacaat ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa
    50341 aggggaaata aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca
    50401 taagcttctt tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac
    50461 aaaagtcttg cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct
    50521 gtgcctccaa aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa
    50581 atttacttta acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca
    50641 tttgaaatcc ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct
    50701 tataaacctc gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct
    50761 ttgtttgcgc agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac
    50821 gtactttatt tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag
    50881 cagtgtaacc gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca
    50941 gttctttttg aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt
    51001 gaccaatgat cggcacaaaa acttccggcc atacttcttg tatgactgcc atcagctcat
    51061 ccagttcatt ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata
    51121 aatgaccttg cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc
    51181 acggtttcaa gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag
    51241 ctactagaca gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt
    51301 ttttaatatc tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg
    51361 ataaaataag ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt
    51421 ccccaatcac taggccaata caactatata aaagcccaaa tccattgcca aacatcagca
    51481 tgcccgctac tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca
    51541 acagaacaaa tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg
    51601 tgctcgatcg aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca
    51661 gaccaataat gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct
    51721 atattcccca catctttttt catttattaa aactgcctcc tt
//
LOCUS       DACECR010000008        49760 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.008, whole genome shotgun sequence.
ACCESSION   DACECR010000008 DACECR010000000
VERSION     DACECR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.008"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            317..3151
                     /locus_tag="IWO17_000411"
     CDS             317..3151
                     /locus_tag="IWO17_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0289310.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            3343..3834
                     /locus_tag="IWO17_000412"
     CDS             3343..3834
                     /locus_tag="IWO17_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ0289311.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3937..4926
                     /gene="manX"
                     /locus_tag="IWO17_000413"
     CDS             3937..4926
                     /gene="manX"
                     /locus_tag="IWO17_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ0289312.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4959..5762
                     /locus_tag="IWO17_000414"
     CDS             4959..5762
                     /locus_tag="IWO17_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ0289313.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5783..6697
                     /locus_tag="IWO17_000415"
     CDS             5783..6697
                     /locus_tag="IWO17_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ0289314.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6780..7154
                     /locus_tag="IWO17_000416"
     CDS             6780..7154
                     /locus_tag="IWO17_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ0289315.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7293..7538
                     /locus_tag="IWO17_000417"
     CDS             7293..7538
                     /locus_tag="IWO17_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0289316.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7710..8774
                     /locus_tag="IWO17_000418"
     CDS             7710..8774
                     /locus_tag="IWO17_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0289317.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9020..9445
                     /locus_tag="IWO17_000419"
     CDS             9020..9445
                     /locus_tag="IWO17_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0289318.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9438..9887
                     /gene="nusB"
                     /locus_tag="IWO17_000420"
     CDS             9438..9887
                     /gene="nusB"
                     /locus_tag="IWO17_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ0289319.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            9984..10838
                     /locus_tag="IWO17_000421"
     CDS             9984..10838
                     /locus_tag="IWO17_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ0289320.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10839..12176
                     /locus_tag="IWO17_000422"
     CDS             10839..12176
                     /locus_tag="IWO17_000422"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ0289321.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12181..12405
                     /locus_tag="IWO17_000423"
     CDS             12181..12405
                     /locus_tag="IWO17_000423"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ0289322.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12407..13297
                     /locus_tag="IWO17_000424"
     CDS             12407..13297
                     /locus_tag="IWO17_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0289323.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13306..14124
                     /locus_tag="IWO17_000425"
     CDS             13306..14124
                     /locus_tag="IWO17_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ0289324.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14231..14680
                     /locus_tag="IWO17_000426"
     CDS             14231..14680
                     /locus_tag="IWO17_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0289325.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14698..16380
                     /gene="recN"
                     /locus_tag="IWO17_000427"
     CDS             14698..16380
                     /gene="recN"
                     /locus_tag="IWO17_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ0289326.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16435..16551)
                     /locus_tag="IWO17_000428"
     CDS             complement(16435..16551)
                     /locus_tag="IWO17_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ0289327.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16624..17580)
                     /locus_tag="IWO17_000429"
     CDS             complement(16624..17580)
                     /locus_tag="IWO17_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0289328.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17751..18119
                     /locus_tag="IWO17_000430"
     CDS             17751..18119
                     /locus_tag="IWO17_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ0289329.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18289..18720
                     /gene="mraZ"
                     /locus_tag="IWO17_000431"
     CDS             18289..18720
                     /gene="mraZ"
                     /locus_tag="IWO17_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ0289330.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18741..19700
                     /gene="rsmH"
                     /locus_tag="IWO17_000432"
     CDS             18741..19700
                     /gene="rsmH"
                     /locus_tag="IWO17_000432"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ0289331.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19705..20106
                     /gene="ftsL"
                     /locus_tag="IWO17_000433"
     CDS             19705..20106
                     /gene="ftsL"
                     /locus_tag="IWO17_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ0289332.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20107..22293
                     /locus_tag="IWO17_000434"
     CDS             20107..22293
                     /locus_tag="IWO17_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0289333.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPSED
                     PDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYRNL
                     EADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVTGW
                     SKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22319..23281
                     /locus_tag="IWO17_000435"
     CDS             22319..23281
                     /locus_tag="IWO17_000435"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ0289334.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23295..24656
                     /locus_tag="IWO17_000436"
     CDS             23295..24656
                     /locus_tag="IWO17_000436"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ0289335.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVSECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24682..25770
                     /gene="murG"
                     /locus_tag="IWO17_000437"
     CDS             24682..25770
                     /gene="murG"
                     /locus_tag="IWO17_000437"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0289336.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25785..27005
                     /locus_tag="IWO17_000438"
     CDS             25785..27005
                     /locus_tag="IWO17_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ0289337.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27161..28486
                     /gene="ftsA"
                     /locus_tag="IWO17_000439"
     CDS             27161..28486
                     /gene="ftsA"
                     /locus_tag="IWO17_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ0289338.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28509..29750
                     /gene="ftsZ"
                     /locus_tag="IWO17_000440"
     CDS             28509..29750
                     /gene="ftsZ"
                     /locus_tag="IWO17_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ0289339.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29767..30444
                     /locus_tag="IWO17_000441"
     CDS             29767..30444
                     /locus_tag="IWO17_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ0289340.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30458..31057
                     /locus_tag="IWO17_000442"
     CDS             30458..31057
                     /locus_tag="IWO17_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ0289341.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31072..31359
                     /locus_tag="IWO17_000443"
     CDS             31072..31359
                     /locus_tag="IWO17_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ0289342.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31408..32190
                     /locus_tag="IWO17_000444"
     CDS             31408..32190
                     /locus_tag="IWO17_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ0289343.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32211..32918
                     /locus_tag="IWO17_000445"
     CDS             32211..32918
                     /locus_tag="IWO17_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ0289344.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33180..35978
                     /gene="ileS"
                     /locus_tag="IWO17_000446"
     CDS             33180..35978
                     /gene="ileS"
                     /locus_tag="IWO17_000446"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ0289345.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36027..37553)
                     /locus_tag="IWO17_000447"
     CDS             complement(36027..37553)
                     /locus_tag="IWO17_000447"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ0289346.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37568..38215)
                     /locus_tag="IWO17_000448"
     CDS             complement(37568..38215)
                     /locus_tag="IWO17_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ0289347.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38399..38728)
                     /locus_tag="IWO17_000449"
     CDS             complement(38399..38728)
                     /locus_tag="IWO17_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ0289348.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38905..39633
                     /gene="sfsA"
                     /locus_tag="IWO17_000450"
     CDS             38905..39633
                     /gene="sfsA"
                     /locus_tag="IWO17_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ0289349.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39649..40662
                     /locus_tag="IWO17_000451"
     CDS             39649..40662
                     /locus_tag="IWO17_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0289350.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40662..41939
                     /locus_tag="IWO17_000452"
     CDS             40662..41939
                     /locus_tag="IWO17_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0289351.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42002..44704
                     /locus_tag="IWO17_000453"
     CDS             42002..44704
                     /locus_tag="IWO17_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0289352.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44856..45173
                     /locus_tag="IWO17_000454"
     CDS             44856..45173
                     /locus_tag="IWO17_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0289353.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45203..45523
                     /locus_tag="IWO17_000455"
     CDS             45203..45523
                     /locus_tag="IWO17_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0289354.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45631..47091
                     /locus_tag="IWO17_000456"
     CDS             45631..47091
                     /locus_tag="IWO17_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0289355.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47159..47380
                     /locus_tag="IWO17_000457"
     CDS             47159..47380
                     /locus_tag="IWO17_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289356.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47411..47593
                     /locus_tag="IWO17_000458"
     CDS             47411..47593
                     /locus_tag="IWO17_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ0289357.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47593..48006
                     /locus_tag="IWO17_000459"
     CDS             47593..48006
                     /locus_tag="IWO17_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ0289358.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48129..49310
                     /locus_tag="IWO17_000460"
     CDS             48129..49310
                     /locus_tag="IWO17_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0289359.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
BASE COUNT        16561 a         8421 c        10747 g        14031 t
ORIGIN      
        1 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
       61 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
      121 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
      181 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
      241 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
      301 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
      361 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
      421 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
      481 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
      541 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
      601 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
      661 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
      721 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
      781 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
      841 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
      901 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
      961 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     1021 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     1081 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     1141 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     1201 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     1261 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     1321 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     1381 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     1441 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     1501 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     1561 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     1621 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     1681 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     1741 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     1801 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     1861 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
     1921 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
     1981 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
     2041 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
     2101 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
     2161 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
     2221 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
     2281 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
     2341 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
     2401 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
     2461 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
     2521 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
     2581 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
     2641 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
     2701 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
     2761 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
     2821 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
     2881 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
     2941 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
     3001 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
     3061 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
     3121 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
     3181 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaaag
     3241 ttggcacgct ttttgcttat agtagtgtgt gctaggaggt ggttgttcac acagaaatga
     3301 taggggtttc tgtgaagtag tgaggaagaa ggagttttgc agatggatat tcgattggca
     3361 cgtatagatg atcgtttaat ccacgggcaa gtcgcaacag tttggtcgaa gcaaacaggt
     3421 gttgaacgta tcattgtgat cagcgatgta gtcgctcaag ataaattgag aaagtttcta
     3481 ctaaaagaag ctgcaccgcc ggggatcaca gcaaatgtaa taaccgtgca gaaaatgatt
     3541 caaatctatc atagtggatt attatcacga gcaaaagtga tgcttttatt caccaatccc
     3601 caagatgttg aagcggttgt ccgctgcggt gtacagttga agtcgttgaa tatcggcggt
     3661 atgagtttta ctgaaggcaa atcaatgatt accaattttg tttcagtaaa tcaacaagat
     3721 attgattcct ttcgttactt gaacagtcaa gaagtggagt tagagattcg aaaagtacct
     3781 gctgacagga aaatcctttt gatggaacta ctagcgaaaa tcaaaaaggt gtagcaagaa
     3841 tggttgttgg aaaaggtcaa ataattctgt tgtaatggtt agcaattatt aatatttttg
     3901 aatttgttac aacaaaagta aataggaggt ataaatatgg tagggattat cctagcaagt
     3961 catggacaat ttgctgaagg aatcttacaa tccggttcaa tgatctttgg cgaacaagaa
     4021 aacgtcaaag ccgtgatttt gaaaccaagt gaaggtccag atgacttgag agccaaattg
     4081 gaagaagcgg ttgcttcttt tgataatcaa gacgaagtgt tattcttagt tgatttatgg
     4141 ggcggaacac cgttcaatca atcaaacaca ttattcgaag agcacaaaga taaatgggca
     4201 atcgtcagcg gtttgaattt accaatgttg attgaagcct acgcatcacg cttttcaatg
     4261 gaatctgctc atgaaattgc agcacacatc attgaaacag caaaagacgg tgtaaaagta
     4321 aaaccggaag aattagaacc ggcagaagca ccgaaagcag cagttgaaga cgcacaacct
     4381 aaaggagcac ttccagaagg aactgtggtc ggagacggaa aaatcaaata tgtattagca
     4441 cgtgttgact cacgtctgtt acatggacaa gttgcgacag cttggacaaa agcagtgcaa
     4501 ccaaaccgta ttatcgttgt ttcagatgca gtatcaaagg atgatctgcg taagagacta
     4561 atcgaacaag ctgctcctcc aggtgtaaaa gcaaatgtta ttcctattag caaaatgatc
     4621 gaagtagcaa aagaccctcg ttttggaaat accaaagcat tgcttttatt tgaaaatcct
     4681 gaagacgtat tgacagctgt tgaaggtggc gtagacatca aagaattaaa tgttgggtca
     4741 atggcgcatt cggtcggtaa agtcgttgta agtaaagtat tgtctatggg acaagaagac
     4801 gttgaagcgt ttgagaagct tgaacaaaaa ggtgtgaaat tcgatgtacg taaagtacct
     4861 aatgattcac gcgataacat ggatgatatt ttgaaaaaag caaaagctga attagcaaaa
     4921 gcgtaataaa taatattaaa atttatagga ggcttatcat gtctattatt tcaataattt
     4981 tagtcgtatt agttgccttt ctagctggta tggaaggtat tcttgatgaa tttcaatttc
     5041 atcaaccact agtggcatgt acattgatcg gtttagttac cggtaacctc gaagctggta
     5101 tcgtacttgg cggtacgctt cagatgatcg ctttaggttg ggcaaatatc ggtgccgctg
     5161 tagcaccaga tgctgcatta gcatcagtag catcagcaat tattttagtt ttaggtggac
     5221 aaggtgttaa aggtgttcct tctgcgatcg cgattgccgt tcctttggct gttgcagggt
     5281 tgttcttaac aatgatcgta cgtacaatcg ctgttccaat cgttcatttg atggatgcgg
     5341 ctgctgaaga aggaaacatc cgtaaggttg aaatgtggca catcattgct gtatgtttac
     5401 aaggggttcg tatcgcggtc ccagcagcag cgttgctatt tatcccagca caaactgtgc
     5461 aatcattcct tgaatcaatg ccagcatggt taacagatgg tatggctatc ggtgggggaa
     5521 tggtcgtagc tgtaggttac gctttggtaa tcaacatgat ggctacaaaa gaagtatggc
     5581 cattcttcgt tattggtttc gttgtagctg caatttctca attaacattg attgctctag
     5641 gtgctttagg tgttgcatta gctcttattt acttgaacct ttctaaaatg ggtggttcat
     5701 caaataatgg aggcggaagc aactctggtg accctttagg cgacattttg aatgactatt
     5761 aatcttgaag gaggaaaata aaatggcaga agaaaaaatc aaattatcaa aaaaagatcg
     5821 tttagctgtt gcgtggcgct ctaccttcat tcaaggttca tggaactacg aacgtatgca
     5881 aaacggtggc tgggcatttt ctatgattcc agcaatcaaa aaattataca aaacaaaaga
     5941 agaacgttct gcagcattaa aacgtcactt ggaattcttt aatacacacc catacattgc
     6001 ttctccaatt ttaggggtta cattggctct ggaagaagaa cgtgcgaatg gcgcgcctgt
     6061 tgatgatgta gcgattcaag gggttaaagt aggtatgatg ggtcctttag ctggtgttgg
     6121 tgacccagtg ttctggttta cagtacgtcc aatgctaggt gcactaggtg cttcattagc
     6181 tatgggagga aatatccttg gaccaatcat cttcttctta ggttggaaca ttattcgttg
     6241 ggcattcatg tggtatacac aagaatttgg ctacaaagct ggttctaaaa ttactgatga
     6301 cctttctggc ggattgttac aagacgtaac aaaaggtgct tcaattttag ggatgttcgt
     6361 attagctgcc cttgtacaaa gatgggtatc aatcaaattc ttaccagttg tatcaactgt
     6421 aaaattagat aaaggtgctt acatcgaatg ggataaattg ccttcaggtg gtgaaggaat
     6481 caagagtgca tttgaacaag ttaataatgg cttagccctt tctcctgaaa aggtaacaac
     6541 attacaagac aacttagacc aattgattcc tggtttagct gcattaggat taacattgtt
     6601 atgtatgtgg ttgcttaaga aaaaggtttc tccaatcgtt atcatccttg gtttattcgt
     6661 agtcggtgtt gttggacacg tcatcggact tttataatcc ataaaaatat agctcgggcg
     6721 ttgtcccgag ctttttttat atagtatact gttcataaac aaatatctgg agggagaaaa
     6781 tggttcaatc aatcaataca aaagtagact tggtaattga tgctacttcg tttacgggtc
     6841 tgacagatta cgggcaaatc atgataggtg ataatggttt tgagttttac catgcgcgag
     6901 atagacgcaa atatatccaa attccatggg aagaagtgga ttatgtacta gcatcagtca
     6961 tgctaaaggg aaaatggatt cctcgatatg ccattcaaac gaaaaaaaat ggtacataca
     7021 cattttcttc caaagaacct aaaaaagtgc ttcgtgcgat cagagaatat gtagatgaag
     7081 atagaatggt acaatcattg agtttctttg atgtagtgaa acgtggtatt cgtagtatgt
     7141 ttaaacgcaa gtaaaaaata aagaaaactt ctaagaggag gttttcttta tttttttact
     7201 ttgcagaaga aaatatgaaa tagcaaaacc aattatttgt cccctttaaa tcccatatgc
     7261 taaaattgat tagcaaagaa ggaggttata ttatgagttg gttatggaca ttgattgttg
     7321 gagccgtgat cggagcaatt gcaggtgcga tcaccagtaa aggaaattcg atgggctgtg
     7381 taacgaatat cattgctgga ctagtcggtt caagtattgg acaagcgatt ttcggcgaat
     7441 ggggaccaaa agctgcaggt atggcactta ttccatctat tttaggcgca gtgattttgg
     7501 tagcagttgt ctcatttttc tttggaaaac gatcataagt ttttacaaaa tagtaaacta
     7561 aaaggagttg cagcgagact gtgactcctt tttggcttta tttatttaaa ataaaaagaa
     7621 gttttccgga aatttttatt taactgtcgt gtacgaagaa aagtttgcta taatggaaaa
     7681 aagcgtgcaa agaaagaggg agtttcatta tgatgctacg tgtagaaaaa ttgcgcaaaa
     7741 aaatgcaaga agaaaatttg gattcatttt tggttaccag tccgtacaat cttcgttact
     7801 taacgaactt tacaggtacg acaggattag ctgttattac tttggaaaaa gcatttttta
     7861 ttactgattt tcgttatacc gagcaggcgg cagcgcaggc tcaagggttt gaaattatta
     7921 aaaatgtcgg acctattttt gatgaggtag caaacttagt acgcaaagaa gaactgcact
     7981 cattagcttt tgaggaaaca acagtctcat ttttagaata ttcggtttta gaagaaatca
     8041 tcgatgcgga attagtacct gtttcacaga tgatcgaaga actcagagaa gtgaaagatg
     8101 aagaagaaat cgctattatc gaaaaagcat gtcatattgc ggatatggca tatgatcaca
     8161 tcctgaaaat gatccaaccg ggcatgactg agattgaagt cgcaaatcaa ctagactttt
     8221 atatgcgttc tcttggcgct tcaggtgttt cttttgatac gatcgttgct agcggattgc
     8281 gttcggctat gccgcatggt gtagcaagca aaaaaatcat cgaacaaggt gacttgatca
     8341 cattggactt tggttgctat tacgaagggt acgtttccga tatgacacgt acattcgcta
     8401 ttggtgatcc tggggaaaaa ttaaaagaaa tttatcaaat agtcttagat gctcaattag
     8461 cagttattga tgctgcaaaa ccaggaatga ctggtgtcca attagatgca gttgctagag
     8521 attatatttc aaaacatggt tacggggaag catttggaca ttctacagga catgggatcg
     8581 gtttagagat ccacgaagga ccaaacgttt cttctcgtgc agaaaagcaa tttgtaccag
     8641 gaaatgtgat cactgatgaa ccagggatct acttaccagg aatcggtgga gtacgtattg
     8701 aggatgactt gttgatcaca gaagatggaa atcgtgtgtt gactcgttca ccaaaagaat
     8761 tgatcatctt atagtttcat caatagaaga agtaagcgta aaaatcagtg ttcacagact
     8821 ttgttataga aaatcactcg atttaatgat acactataga agaactttta agctgtaaga
     8881 gataataaag ttgggtgttt tctccaattc atagcagttt gcagctgatc ggcggcagtt
     8941 ttcttaccac gtgatcacta tgccgcatca tagagaattc ggatgtatct cgtgcttagg
     9001 caaagggagg aataaataaa tggcagatga aaaaaatttg attttaaacg caaaccaaga
     9061 tttaggagag atcgtgatcg ctcctgaagt catcgaagta attatcggga ttgcagcttc
     9121 taaagtagaa ggcgtatacg gaatgcgcgg aacttttgcc agcaatgtca ctgaattatt
     9181 aggacgtgca gctcacggaa aaggtgttta cttagcaaac gaggaagatg gcttgaaagt
     9241 agatctttac tgttatctag aatatggtgt ttcagttcct aaagtagcaa tggaaatgca
     9301 ggaacgggtc agccagcaag tccttttcat gacggatatc gatttatctg aagtcaatat
     9361 ccatgtagta gctgttgttc ctgaaaaatt agaacaacca acaatcgaag aactttttga
     9421 agacgaagag gaagagaatg aataaagaat tatcaagaca cgagattcgg gaaatggcat
     9481 tgcaggcttt atttccatta gacttcaatg cagacctaac aaaagaagat gcgattttca
     9541 acgcaatcga attagaccac cgcgacatga tcaacgaaga tgaatcagag tttgttcctg
     9601 tttatcttga tacgctagtt ggcggtgtct gtgcaaaaaa agatgaattg gataaagtga
     9661 ttgaaaaaca tttaaaaaac aattggcaca tcaatcggat ctcaaaaatg gatttggtga
     9721 tcttgcgtat cgcgattttt gaaatgactt atgtgacaga tgtaccagcg gctgttgctt
     9781 taaatgaagc aatcgagttg gcaaaaacat ttagtgatga ccgttcacgt aaattcgtca
     9841 acggtgtttt atccaatgta ttgaaagaat tagaagctgg ggcgtaagtc ctagcttttt
     9901 tctatgaatc atcataaacc tgttataaat cgggaaaatt ctatggtaga atgaaaacac
     9961 aaaaaatatc tgggagtggt tagatggcag aattgattaa cggaaaagaa ttagctgaaa
    10021 aaatgcaggc agagattgct gaaaaaatcc aaaaattgaa agataatggt atccatcccg
    10081 gattagtcgt tttattggta ggggaaaatc cagccagcca aatctatgta agaaataaag
    10141 aacgtgtagc aaaagaaatc ggaatccatt cattggtaga gcgttatcct gagacgattt
    10201 ctgaagacga actacttgca gaaattgaaa aatacaatca agatcctagt tttcatggta
    10261 tattggtcca attaccattg ccaaagcata tagatgaaga aaagatcctg ttagctatcc
    10321 gccctgaaaa agacgtagac ggttttcatc cgatgaattt aggcagactc cttgcgggta
    10381 aaccggacaa gattccttgt actccatacg gcatcatgaa aatgtttgaa gcttattcta
    10441 tcgatccggc gggtaaacga gcagtagtta tcggacgaag caatatcgtc ggtaaaccga
    10501 tggctcagct actcttgatg gcagatgcaa ctgttactgt tgctcattct aaaaccgaaa
    10561 atctggctga actggcacaa gaagcagata ttttagtcgt agcaatagga cgtggacatt
    10621 ttgtcacaaa agaattcgta aaaccaggtg ctgtagtgat agatgtaggg atgaatcgtg
    10681 atgctaacgg taagctgatc ggtgatgtga aatttgatga agtggagcct attgcaggct
    10741 ggatcactcc tgtgccaaag ggtgtagggc caatgactat cacgatgctg atgtatcaaa
    10801 ccgtaaaatc tgccgaagct tttggagcag gtgaatagat ggcacaagaa tatttaactg
    10861 tcactacatt aacgaagtat ttgaaaagaa aatttgatgc cgatccttat ttagaacgcg
    10921 tatatttaac aggcgaaata tcgaatttcc gtttacgtgc caatgcccat caatatttca
    10981 gcttaaaaga tgaccatgca aaaatatctg ctattatgtt caaaggagca ttccaaaaat
    11041 tacgattcca gccaaaagaa ggaatgaaag tccttgtcgt tgggcgtatt tcattatatg
    11101 aagcgggagg ttcttatcaa atctatgtag agcacatgga gcctgatgga ataggtgctt
    11161 tatatcaaga attggaagaa cgaaaagaaa agctgatgaa ggaaggcctt tttcaagggc
    11221 ctaaaaaaat gctgccacgt ttcccaaagc gaatcgcggt ccttaccagt ccaagcggag
    11281 cggttatccg cgatatcatc acgacggtga aaagaagata tccaattgtc caattggttt
    11341 tgttcccaac agttgtacaa ggaaaccaag cagcggacaa tgttgtcagg aatatccaga
    11401 gagtagaaga gatgggcaat ttcgatacaa tgatcatcgg tcgtgggggt ggttcgattg
    11461 aggatttatg gccgtttaat gaagagcggg ttgctcgggc gatttatcaa gctaatacgc
    11521 cggtgatttc atctgtagga catgaaacag ataccacgat cgctgattta gtagcagatg
    11581 ttcgggctgc cactccaaca gcagctgcag aattagctgt tcctgttttg tcagaagagt
    11641 tgatgcgcat tcaagaacgg caagctagat tgcagcaggc gtttgtacgc cagatccagc
    11701 gcaaacaaga acggttcgag cgtgctcgac gttcctatgt atttcggcag cctgaaagat
    11761 tgtatgaagg tcagaccatc aaattagacc agttgaaaca gcgattatat caagctacac
    11821 agcaaattta tcatgcgaaa gaaaaacaaa cgatcgcatt aagccaccaa ttggaacaag
    11881 tggctccgct ttatcgagtt aaagcagctc gccaagaaac tgcttatcta gaaaaacgct
    11941 tagaagagaa aatccaacaa tatatgaaac aacagcagca gcgttttgct caagctgttc
    12001 aatctcttga tttgttgagt ccattgaaaa tcatgggaag aggctacagc tatacgacgt
    12061 taaatgatca agtagtaaaa agtgtcaccg agattcatca aggggatttg ctgacgatcc
    12121 attatcaaga tggaatcgtt aaaggacaag tacaagagat agacaaagga gaataaaaat
    12181 atggaaaatc caacttttga agagtcactg caagaattag aaaaaattgt catgcagtta
    12241 gaacaaggag acgttccatt agaatcagct ctagattcgt ttaaacgagg aatggaatta
    12301 agcaaacatt gccaagatac attagctaaa gcagaagaga ccttgacgaa aatgatgaca
    12361 gaatcaaatg aagaagttga ttttgacgga aacgaggaaa gctgagatga gattaactga
    12421 cttttcagct attcatctac ctgctgtaga aaaagaaatc ttgtcttttt tagatgagca
    12481 tacaacggat agaggactct atgatgcgat gagttattca gtgaaagcag gcgggaaaag
    12541 aatacgtccc ttattacttt taacggccgt tgcttccttt gatgagccta ttgatgttcc
    12601 cgtctatcaa gtagcagctg ctttagaaat ggtgcatacg tattccttga tccacgatga
    12661 tttgcctgcg atggacaatg atgatcttcg tagaggaaaa cctaccaatc ataaagtatt
    12721 cggcgaagca ttagctatat tggccggaga tggtttgctt acaggagcat tccagctaat
    12781 cagtatggca cacttaggta attctcctaa gcttctgctg cttcagcaat tagctgtttg
    12841 tgcgggatca caaggtatgg ttgctgggca agctgcagat atcgagggcg aatcgaaaaa
    12901 gctttcttta gaagaattag catttattca tgaaagaaag acagggcatt tgattcgtta
    12961 tgccttgctg gctggtggca tattagcgaa gcaacctgaa gaaatcttat tgctacttca
    13021 acgattagcc gaacatttag gtttagcttt tcagataaga gatgatctac tcgatgtgat
    13081 tggtacaacg aaaacattag ggaaaacagc tggtaaagat gaacgtatgg aaaaaaatac
    13141 ttacccgcgt ttattaggcc tagaaaagac acgtgaggcg ttggagattg agctgttatc
    13201 cgccaacaag atcatagaca aattagaaga ggacgttttt tcttttgatg gcgggctgat
    13261 gcgccagatg atcaagcaat ttgatgtgga gaaatgaggg aaagaatgga aaaagaacgc
    13321 gtagatgtat tagcggtcaa acaaggatta tttgaaacaa gagaacaagc caaacgttca
    13381 gtgatggctg gtttgattta caacgaaaaa aacgagcggt ttgataaacc gggagaaaaa
    13441 attccagtga gtagtgaatt aagagtcaaa ggtaaaaaac ttccttatgt ttctcgaggg
    13501 ggattaaaat tagaaaaagc actcaaagca tttgatctat ctgttgaagg aaaaacattg
    13561 ttagatattg gtgcatccac tggcggattt actgatgcag ctttgcaaaa tggcgcaaaa
    13621 atgagttatg ctttagacgt tggctacaat cagctagctt ggaaaataag acaagatcct
    13681 agagtcgtcg taatggaaag agtgaatttt cgttatgcaa aacccgaaga ctttacctta
    13741 ggaattccag aagttgctgt tatcgatgtt tcttttattt cactaaaact gatgctgcct
    13801 ccattacatg ctattttaaa agaagaggga gaagtgattg ctttaatcaa accccaattc
    13861 gaagcaggca gagaagctgt agggaaaaac ggaattgttc gtgaccctca gacccatcaa
    13921 aaagttttag aagacatttt gtcatttgcg gcacaaggtg gatatgatgt gctggaatta
    13981 agttattctc cgatcacggg gggagaagga aatatcgagt tcttggctca cttgaggaaa
    14041 gtacccgaaa gcggaacaat caattcagct attaatatgg cagaagtggt ttcaaatgca
    14101 cacgaacagt ttgaccataa ataaattgta actgtgaaaa tcaagtgaga agtatgcgga
    14161 ttcttcttta ttataccaag gaaataagct atcattgatt aagaagaata tgctaaggag
    14221 cgaagacgta atgagaaaaa aagatcggca tcgtttgatt actcgattgt tgagtgagca
    14281 agatatccga aagcaggaag aatttgttga aatcctgaaa agcaaaggaa tatctgttac
    14341 tcaagctacg atttccagag atataaagga actgaaactg atcaaggtac cggcttcaga
    14401 tggcggctac cgttatagca taccagctga aacaaacgaa gatgtaaata ctaaattgga
    14461 aaaactatta aaagacgctt tcgtggcagt tgaccaaatg gaaaaatttg ttattttgaa
    14521 aacattgccg ggaaatgctt cggcagcagc aaatttgatt gataaacgtt acaagaaaga
    14581 attattttcg attatcaatg atgacgataa tgttttgatg attacacgaa ctgaagacca
    14641 ggcagtttta ttgaagaaag acttcctgca ttacctttaa gttgaggagc gaatgtgatg
    14701 ctacaagaaa tcagtattac taactttgcg attattccgg aattgcggct ttcttttcac
    14761 gaaggaatga ccgcgctgac cggagagact ggtgcgggta aatccattat tatcgatgct
    14821 ttaggtctgt tagcaggagg aagaggttca agcgattata tacgtcaagg agcagaaaaa
    14881 tgtgtgcttg aagggttgtt tgaactacca aaacaagaag gattctctga actgatggtt
    14941 gaattaggga tcgaaacaga tgaggacaat ctgattgttc gacgggatat gtccttaact
    15001 ggaaaaaatg tttgtcgagt gaatggtcat attatcacat tagctaatct gagaagaatc
    15061 ggcagttatt tagtagatat ccaaggacaa aacgaacatc aggaactgct ccagcctgaa
    15121 tcacatcttg ctttattgga tcgttttgga gatgtagctt tccaacagaa gaaaaagagt
    15181 tatcagcaag aatatatctc ttatcgtgag ctggaaaaaa gggtacgaaa agtgcagcaa
    15241 aatgaaaaat cctatgttca gagaatagat atgctccatt tccagcaaga agaaatagct
    15301 gctgctgatt tgcaagtcgg agaagaagag aaattaaaag aagaaagaga aaaacttagc
    15361 aattatcaaa aaattgctga tggattagct gcaggttacg gagcactgac gaataatgaa
    15421 caaaacagtg tagatggtgt tggcttggcc gtttcagaac tgcaagggat cgcccatcta
    15481 gatgtggagt atgaagcaat ttatgaaaat atccaaagtg cttactattt gctgcaagat
    15541 gcaatcggcg atatgagtcg tcagattgat ttattggagt tagatgagag tcgtttagaa
    15601 gaagttacgc aacgtttaga attaatccga cagttgaaac gtaaatacgg cgaatccatt
    15661 gagtcaatat tggcttacta tgacgaaatc accgaagagc tagcttcttc tgatttttca
    15721 gaaggtcaat tggacaaaat gaaagaggaa ctagagcaaa aagagcttct acttcagcaa
    15781 caagccgcag atcttcatga agcgcgtaaa aaaatcgcaa aagaattgga aaaatccatt
    15841 ctgcatgaat tgaaaagtct gtatatggaa aacactgagt ttgaggtacg tttcttaaaa
    15901 gaagaaaata gacaattgaa tcatgatgga tttgaccaaa tcgaatttta cattacgacc
    15961 aatccaggag agccactgaa gcctttggtg aaagtcgctt ctggtggcga actgtctcgg
    16021 atgttgcttg ctttgaaaac tattttttct tctgagcaag gtgtgacaag tattattttt
    16081 gatgaagtgg atacaggtgt gagcggaaga gttgcacagg ccatcgcaga taaaatttct
    16141 caaatatcga aatactctca agttttgtgc atcactcatc tgcctcaagt tgctgcagtc
    16201 gccgactatc aatattatat tgtgaaagca gtgattgatg gacgtacgca aacttctgtt
    16261 tcagagttga aaacaaaaga aagagaagaa gaaattgcac gtatgctggc tggaagtgag
    16321 attacaaaac tcacactcga acatgcaaaa gaactattga aactcgcaaa aaggacctga
    16381 gccacctcgc agtggttcag gtccttttca gcagctgata gctatatttt atttttacat
    16441 gacactcatt acagcagtca atacaacaga tccaatcgtt aaaatcagca ttgtccaaat
    16501 gacaaatttt gttactttag caaaagtact tgtttgtttt ttttcacgca ttgttttcaa
    16561 cctcgtcatt aattagttga ttcaagtgta caatcttttt tccatttcca caagagggct
    16621 tttttataaa aacttccctt tttttaaata ataggtagtt acgatacaaa taaataatgt
    16681 taatagaact agccatataa aggcatcatc cctttcagca ataggtaact taatgttcat
    16741 tccatagata ctgccgacga ttgtcggtat agttaaaata atcgttaaag aagttaaaat
    16801 tttcataata ttattcagat tattcgatac gattgctgaa aaagtctgat tcatttgttc
    16861 tactagtttt aattgaatgc gagaagtagt cattgcctgc tctgtttcca ccaatacatc
    16921 acgtaaatga tttttcaaag catgattttc ttggaaaagt ttattttgcg ctagttcttt
    16981 caatgcatct aaattagcag aagtcgcttc tctgaaataa acgaggcttt tttggatatc
    17041 catgatttga taaagttgtt tgttttcagt ggatacttga agtttggctt ccaaagtatc
    17101 gcattgagat tgaagcgtag ctaaataact gttgtaagag agtgtgacct gccaaatgaa
    17161 ttgaaagaag atattgagat taggcgaaat atcacttttt ggaaaggtct ggctaaagac
    17221 tttctttaaa aacagtggtt catgattcgt aattgtgata agcttctttt tgggcgtcaa
    17281 aataatagag agcgggtatg tatccacttg aaaataaccg ctaggacttg ttttagcaaa
    17341 aggatattgt aacaataata acgctggctt tgtcaacttt gattgattga attcttctgt
    17401 acgggaattt tctgcatcgt ccaatatgga agtgatgtaa tctgacggaa tttcaaattg
    17461 atgtgtcagc tgatgaatct cttcttgatt aggacgttct acacaatacc agatcgtttg
    17521 gtcagtttcc tttccggtat tacgcagttt ctcattatct aatgtaaaat atttgatcat
    17581 gattttcctc ctgttgcctg tttcttttat tataacggat agggaattgg aatacatgga
    17641 atgtgtttgt aagatataga atataactgc ataaatttaa ataaaatgaa acagttgtgt
    17701 aaagtatata cagtatactt gaagcaacga taaaaaagga gtttacgttc atgggcacat
    17761 tcacaccact tatcttgttt tggtatattt acccggtaat cgttttgttt ggttgccaat
    17821 ttcttgtaaa attattttcg ttaaatcgtc gttttaaaat aaaagcccct gatttggcga
    17881 ttcctttttt gtggggcggt atgcatgcat tgtctagaaa tacagggaca atttctatct
    17941 taccgttttt ctttatttct gttttgctaa tgggaatttg tattgctgtt ttccaagcat
    18001 attattatga agaaattatt tatccgcgtt atttcaagat gacttggcgt ttggtttttc
    18061 tgttgacaat cgtcttgtac gctgtcttga tcattgtgaa catactttct tacctatgaa
    18121 aaaaatagaa aagctgtaaa aagtctttta aaaggctttt tgcagcttga tttttaaaaa
    18181 aatgtttatg aataaaaaaa gttgttcccg gatttcccag aaaaggtggt agaaagtggg
    18241 ggattgtggt agactaattt tagttagtgg aggaatgggg ggctttcaat gttgatgggt
    18301 gaattccaac ataatattga tgcaaaagga cgtttaatcg tgccctcaaa acttcgtgaa
    18361 gaacttggcg aaaagtttgt tctgacacgc ggattggatg gctgtctttt cggctatccg
    18421 atgagcgaat gggaaaatct ggaagctaaa ctgaacgaaa tgccgttggc taaaaaagac
    18481 gcacgtactt ttgtgcgatt cttctattcg gctgccactg aatgcgaact tgataaacaa
    18541 ggacgaatca atatacccag cacgttaaga aattatgcag ctttgacaaa aggttgcgta
    18601 gtcatcggtg tatctaaccg aattgaaatt tgggatgaga cacgatggca agatttttct
    18661 gcagcagcag aagagaactt tgacgaaatt gctgaaagta tgattgattt cggactataa
    18721 gaataggaag aggctgacta atgactgaaa catttcaaca ctatacagtc atgttgaaag
    18781 aaacggtaga tggattgaac atcaaaccgg atggcgttta tgtagactgt acactcggcg
    18841 gtgccggaca tagtgaatat ttattaagcc aactaagttc taaagggcat ctgtatgcgt
    18901 ttgaccaaga tcaaaaagca atcgatcacg ccaagctgcg tttagcttct tatgtcgaac
    18961 aaggtatggt cacatttatc aaagcgaact tcagagaatt agaagaacgc ttgagtgagc
    19021 atgtagataa ggtagacggc attttgtatg atctaggggt ttcctctcct cagctagatg
    19081 aagcagaacg tggatttagt tatcatcaag atgcaccgtt ggatatgcgc atggatcagt
    19141 cagctccttt atccgcttat cacgtaatta atgaatattc ctaccatgaa ttagtaaaga
    19201 ttttcttccg ctatggcgag gagaaatttt ccaaacaaat tgctcgtgaa atcgaacgag
    19261 tacgaaaaac aaaaccaatc caaacaactg gtgagcttgt cgaaatcatt aaacaagtca
    19321 tcccagctcc cgcaagaaga aaaggcgggc atcctgcaaa aagaattttt caagctgtgc
    19381 ggattgcagt aaatgacgaa ctcggtgccg aagaggcctc tttagaacag gcaatccggt
    19441 tattgaaagt caatggaaga atcagcgtaa tcacattcca ttcattggaa gacagaattg
    19501 tcaaaagtat gtttaaagaa tatagtacgg tacaagatct accaccggga cttcctgtca
    19561 tacctgacga attccaacca gaactgaaag tcattactcg taaaccaatc cttccttcag
    19621 aggaagagct aacagaaaac aatcgatcac gcagtgcaaa gttacgtatt gcagaaaaaa
    19681 taagaatgaa agaggagtag aatgatggca gaattaaaga aaatgcaaga ttacgaatat
    19741 gatttacctg aattggtgga tcagccggaa caacaaatgc ctgattcttc ttcgaatcaa
    19801 gaaatcattc ttccacaatc accggcaaaa cgattaaagc agatttctgc tttggaaaaa
    19861 attgctgtgg caagcgtggc gattgctatt gttgctttat gtgttttaac agttatgctg
    19921 cgcacaaata tcagcggagc tgaaaaagaa atcacgtcaa tacaaacaga aatcaatgat
    19981 aaaaagcaag aaaaaacgag tttagaacaa gaaaaaaacg aactttctcg tactgaacgg
    20041 attaaacaaa tcgcagaaga aaaaggattg tctattaatg acgacaattt aaggaaagtg
    20101 aagtaaatga gtcttaaaaa taaaattcgg cggtttatgg aaaagaaaaa tctaaatccg
    20161 atgaacaatc gcaaaaaagt aggtatcatt ttatttgcta cgagtattgg attgttcttt
    20221 ttatttgccg tccgtttctc gtatatcgtt attggaggtc atgttgctgg tacctcatta
    20281 gctgaaaaaa caaaacagtt gtaccaagga agtgaagtag taaaagcaaa aagaggaacg
    20341 atttatgatc gtaacggtgt ggcgctcgca gaagatgcct cctcttattc aattaaagcg
    20401 atactctcaa aaacatatac ctctggggat aaaaaactgt atgttgaaga aaagaatttt
    20461 gataaaatag cagaaatcct gcataagaat ctttctatag acaaaaaaga tgcactgaat
    20521 attttagagg acggcgcaaa aaaagagctg tatcaagttg aatttggtag ctatgggaaa
    20581 aatatctccc aagaaaccaa gcaaaacatc gaagcggaca tgaaaaaaga aggtgtcgct
    20641 ggtctttatt ttgtcgacca tcaggctcgg atgtatccaa atggcgtctt ttcttctcat
    20701 tttattggat acgcagttcc agacaaagat gagaatggct tagttggtaa actaggtttg
    20761 gagtctgctt acaatgatat cttgagcggt aaagatggta agattattta tcaaaaagat
    20821 aattttcaaa atcctctgcc aggtacagtg gcagaagaag aaaaagctgt cgacggacaa
    20881 gatatctaca cgactttaga cagcagacta caaagttatt tagaaacact gatggatcaa
    20941 gtaaatgaag aatatcagcc agaagaattg acagcagtcc tgatgaaagc aaaaaccgga
    21001 gaaatcttgg caatgggaca acgacctact ttcaatcctg aaacaatgga aggattgact
    21061 ggaaaagatg ctatctggcg taatttcttg gtacaagata gttatgaacc aggatcaacc
    21121 atgaaagttt tcactactgc agctgctatc gaagaaggag aattcaacga gaatgaaacc
    21181 ttccaatctg gaaaaatcca agtagaagat gctacgatca atgaccatga ctttggtgaa
    21241 aaaggtgtgc tgaccatgcg tcaagctctt tcatggtcaa gtaacgtcgg gatggtcata
    21301 ctggaacagc gattaggcgg acgatggtat aactatttac aaaaactggg atttggacaa
    21361 agtactcatt ccggattaga tgatgaagta aacggtgctt tacctacatt aaatattgtc
    21421 gaccgagcaa tgagtgctta tggacaagcg gtaggtgtga cgaatttcca aatgatgaaa
    21481 gctttcactt ctattgcgaa taacggtaca atgattcaac ctcgatatat cagcaaagtg
    21541 gtggatcccc aaactggaga agagcgaaca actcaaacag aagttctagg gcaacctttt
    21601 tcaaaagaaa caacagaaaa agtccgtgaa tatatgagag atgttgtcga aagcgaaaac
    21661 tacggtagtg cttatggtgt tgtacctgga tacaatgtat cggcaaaaac cggaacagcc
    21721 caaatcgctt ctgatacagg gggatatcaa acaggagata cagcctacct gtattcgatt
    21781 gtggagatgg ttccttctga agatccagac tatgtcttgt atctaacaat gaaacaccct
    21841 aagacatatg atcgaatggc attggctaaa atcgcaaatc cattgatgaa acgggcaatg
    21901 gattttaaag aaactgaaga agattcagat acagaaacaa aaacagaaaa agtatccgta
    21961 gccgattatc gtaatcttga agcggacgtt gcagcagctg atgctcaaaa aagtggactg
    22021 caacctgtag tgatcggtaa tggtaaaaaa gtacaaaaac aatctactgc caacggtgat
    22081 cagttgatct ctggagaaaa actgatccta tatactggtg gtgataagct gatgcctgac
    22141 gtaacaggat ggtcaaaagc agatatcatg aagctgggga aaatcctcgg tattgaagtt
    22201 agttttgatg gagatggtta ttgtgtcaaa caagaacttg ctccatatga aaaaatcact
    22261 gaagacaaac ttagttttac tttagaagaa taagaagaga aataggagag aaaagagcat
    22321 ggaatggaca caagcattga ttcccattgt cagcagttgt gcaatgacaa ttgccgcaat
    22381 gccgctattt attggttatt ttcaaatgaa aaagcaaggt caggcgattc gtgaggaggg
    22441 accaaagtgg cacaatagca aagccggaac tcctacaatg ggcggcttag tgtttctgat
    22501 cggctctatt ttgacaggaa tttgggttgg agcttggcaa aaacagctaa ctcctactct
    22561 tttcatcttg ctttttgtat tagcgttata cggagtaatc ggctttcttg atgattttat
    22621 aaaaatattc aagaaaagaa acatgggact gaactcgaag cagaaattga ttggacagat
    22681 tctaggggga atcatctttt atcttgttta ccgttctgaa ggttatccag gtgtattgaa
    22741 tttcttcgga ctagatctac ctttaggaat catttatggt attttcgctc tcttctggct
    22801 tgttggattt tcaaatgcag taaatctgac agatggcatc gatggattag ttgctggctt
    22861 aggaagtatc tcttttgcag cttacggtct aattgcttgg catcagcatc agtatgatgt
    22921 attaatcatt tgcttaagtg ttttaggagg tttactgggt ttcttcgtct acaacagaaa
    22981 acctgccaag atttttatgg gagatgtcgg ttcattagcg ttgggcggac ttttagcggc
    23041 aatttctata atgctaaacc aagaatggac attgcttttg attggtttga tctatgttat
    23101 ggaaacagca agtgtgatgc ttcaagttac ttcctttaag cttacaggaa aacgaatttt
    23161 taaaatgtct ccaattcatc atcactttga aatgtgcgga tggtcggagt ggaagattga
    23221 tacagttttt tggttgatca gcattgtgac ttcgttgatc acattatgga tcgtttggta
    23281 ggagatgacg ggaaatgaaa aaaatatcaa catatgaaaa taagaaagtg cttgtcttag
    23341 gactcgcaaa aagcggagta agtgcggcca aattattaca tgaattgggc gcacttgtca
    23401 ccgttaatga tggaaaacct tttgacgaaa atccggaagc acaagagctg ctttctttag
    23461 ggataaaagt aataacaggt agtcatccaa tcgaattgtt ggatgaagaa ttttcattga
    23521 tggtcaaaaa tccagggatt ccctacagtc atcctttagt agcaaaagcc caagaaatgg
    23581 gtattccggt aattacggaa gttgagctag cttacgaagt atcggaatgc ccaattattg
    23641 ggattacagg aacgaacgga aaaacaacga caacaacgat gacaggacta ctactgaatg
    23701 caggagcaga tcaaggaatt gcacgtttag cgggtaatat tgggtatcca gcaagtggtg
    23761 tagcgcaaga agcaaaatca gaagataaaa ttgtgatgga attgtctagc ttccaactca
    23821 tgggaatcac agatttccgc cctcatatag cggtgattac caatatctat gaagcacata
    23881 ttgattatca tggtacaaga aaagaatatg tcaaagctaa atggaacctt caaaaaaaca
    23941 tgacagaaaa agattatctg atccttaact ggaatcagtc agaactacaa gaattagcgc
    24001 aaagaacaaa agcacgcgta ctgcctttct cgacaaaaga agtgttggaa gacggtgtgt
    24061 atgctgacga ttactcaata tactataaaa aagaaaaaat aatggaaata tctgaacttg
    24121 gcgtaccagg taagcataat gtagaaaatg cactagctgc tatttctgta gcaaaattgt
    24181 atggtatatc aaatgaagct atacgagaaa cgttgcattt tttccatggc gtaccacatc
    24241 gtacgcaata tgtaggagaa atccaaggca gaaaatttta taatgattca aaagcaacca
    24301 atattttagc gactaaaatg gctctaagtg gatttgaaac gtctaaggtc gtgttattgg
    24361 ctggcggatt agacagagga aatacatttg atgaattaat tccttcatta aaaggaatca
    24421 aagcaatggt cgtatttggc caaacaaaag aaaagttgat ggatgctgga aaaaaagcag
    24481 gcatcgagac aatagtaaca gccgattctg tagaacaagc agttccttta gcattagaaa
    24541 acagtacaga tggtgatgta gttttattat caccagcgaa tgccagctgg gatcaatacc
    24601 caaattttga aactagagga aacagattca tggaagctgt gaaccgatta aaatagaaaa
    24661 agtagaagcg agcgataaaa aatgaaaatt ttggttacag gcggaggaac agggggacat
    24721 atttatccag ctcttgcctt tgtcaattat gtaaagactc aagaaccgaa tgctgaattc
    24781 atgtatgtgg gagcaaaacg aggtctagaa aacaaaatcg tccctgatac aggaatgcct
    24841 ttccatacct tggagattca aggatttaaa cgaaaaattt ccatgcataa catcaagacg
    24901 gtccgattgt ttcttaagag tatccgtcaa gcaaaaaaaa tcctcagtga ttttcaacca
    24961 gatatcgtta tcggtactgg aggatatgtt tccggagcgg tagtgtacgc tgcagccaaa
    25021 atgggtattc caacagttat ccatgaacaa aacagtgtac caggtgttac caataaattt
    25081 ttaagccgat atgtggatcg gatcgctctt tcatttaaag atgcggcttc cttttttcct
    25141 gaaaacaaag cagttcttgt aggtaatcct cgagcacaag aagtagcaga tactaaaaaa
    25201 tcagatattt tgaaaacatt tgatttggat ccagaaaaga aaacagtctt gatttttggc
    25261 ggtagtcaag gagcacttaa aatcaatcaa gcagtatcag tttttttaag tgaatatatt
    25321 ccttcttctt accaagtttt gtatgcttca ggagatagat attatgaaga gatcctaaat
    25381 aaaataaatc ctgaaataca aaatattagt gtccagcctt atatcaaaaa tatggcagag
    25441 gtcatggctt ccagtgatct gctggtgggg cgtgctggtg caacttcgat cgcagaattc
    25501 acagctctgg ggttaccggc agtgttggtt cccagcccat atgttacgaa tgatcatcag
    25561 actaaaaatg caatgagtct tgttcacgca ggagcagcaa aaatgattgc agataatgaa
    25621 ctaacaggtg aatcactaag tcagacagtc aacgaaatca tgggtgatga agaattgcaa
    25681 aagcaaatgt gccgagcttc taaagaacaa ggaatccctg atgcttcaaa acgtctttat
    25741 gacttagtaa aacaaataat caaaaaataa aggcggtggt cttagtgagt aagaaaaaag
    25801 aaccaaaaaa attgtctcat ccgtcttctg atgaaaacaa tttgactcct tggcaaaagg
    25861 ctaaccgaaa atatctggca gaacaagaga tgaaaaacca agagccaaaa aaagaagaca
    25921 taacattaga tacacccctt tcagattctg aagaagaaaa aacggttcaa gaggaacagc
    25981 aagaagaaag ccagtccgag aaaaaaatcg aattttttga agagatcaag caaaagaaaa
    26041 acggtggacc atataacggt tcttttttga accggctgcc caatttaaaa agtcaacggc
    26101 acaaagtact atttcgtcgg ttagctttga tcatcactgt attgggtata ccgctgattt
    26161 ttttgatcta ttatgtttct ccttatagca aattacaagc agttgcagta tcaggcaata
    26221 agacagtcaa cagccaagaa atcatctctg atacaaaatt gtcattagga gaaaatgttt
    26281 ggggacagta ttttcatcga tctacttata tcgatcgcct taaaaaagca cagccaagaa
    26341 tcgaaacagc aaatatacat ttcaaaggga tgaatgaatt cgatttggat gtaacagaat
    26401 ataaagagat tgctttgatc gcaaaaaaca atcaatacta cccagtgatt gaaaatggaa
    26461 cagtattaga tgaaaaagta gcaaatccta caaaaaatct ccctatcttg gaagagttca
    26521 aagactctgc aaaaatcaaa gaattaacaa agcaatacaa tcagttatca tcagaattac
    26581 aaaaagcaat ttctgaaatc aagtacacgc caagagcaag caataaaaat ctgatccaat
    26641 tgaatatgaa tgacggcaat caagttattg taaatatcaa taatcttgcg aaccaaatga
    26701 aatattattc gcaagtagcc aaagatatgg atgaaaaagg tgtgatcgac atggaagttg
    26761 gtatcttctc gcatccttac ggtagcactc aagaaacgga agaatcgggc caatcggaag
    26821 aaacaactga aagtgaagaa aatgcggatc aaacaaacga atcaagtgaa gaggcagaaa
    26881 cgcaaaaagc acaaacacct ggatcgtcta gcacaaacgg atcatctgat tcaaatgatt
    26941 caagtgaaac aagtcaaata gaagataatc caagtacaac tccgtcaagt ttaaatgaaa
    27001 attgataaaa caactttaaa aagtaagaat gtactttcta aaaaccagta atttatggta
    27061 gaattggaag cagtgaatat atctattaat aagtatattt taaaacggac gctagttgtt
    27121 cgcttgtata tggaatagta agtaggaggg aatcccattc atggcaaaaa caggaatgta
    27181 tgtaggcctt gatattggaa cgacatctgt taaagttgtc gtggctgaat atatcgacag
    27241 ccaaatgaat attattggag taggaaacgc aaaatcagaa gggattaacc gaggcattat
    27301 cgttgatatc gataaaacgg tccaagcgat acaacgagca gtacgacaag cagaagaaaa
    27361 agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca gcaaacatgc ttgaagtaga
    27421 aaattgccag ggaatgattg cagttaacgg agattcaaag gaaatcaccg atgaagatgt
    27481 cagaaatgtg gcttcagctg cccttgttcg ttcaatacct cctgaacgtc aaatcgtgtc
    27541 aatattgcca caagatttta ctgtcgatgg gttcgaggga atcaaagatc ctcgtggaat
    27601 gatcggcgta agattagaaa tgtacggtct gttgtttaca ggacctaaaa cgatcatcca
    27661 taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc aacgaaatgg tgattactcc
    27721 tttagcgctg actgaatcaa ttctttctga cggagaaaaa gattttggta cgatcgtcat
    27781 tgatatgggt ggcggccaaa caacaacggc tgttatgcat gataagcaat tgaagtttac
    27841 caatcttgac caagaaggtg gcgaatttgt cactaaagat atctctattg tattaaatac
    27901 gtcatttaac aatgcagaag cgttgaaaat aaactacggg gatgcatacc cagaacggac
    27961 atcaccagat gaagaatttc cagtagatgt gattggtcag tcagaaccag tgaaagtcga
    28021 tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa cagattttca acaaagcaaa
    28081 agatgcattg gaccaaatcg aagcattaga gttgccaggt ggcgtcgtat tgacaggcgg
    28141 agctgctagt cttcctggtg ttgtggactt ggcacaagaa atcttcggcg taaatgtaaa
    28201 attgtatgtt ccaaatcaca tggggctgag aaacccggta tttacaaatg taataagtat
    28261 cgtggattat tcagcaaact tgagtgaagt gtatcaattg gcaaaaattg ctgttacggg
    28321 tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa acaacaaaca catatgagag
    28381 ctatgaagca cctgaagaaa caatctacga tgagccggaa ccaaaagagt ccggagaaaa
    28441 tgtaaaaaat aaaatcaagg gtttctttac aaatattttt gattaattca tcctgggagg
    28501 aataagatat ggaattctca attgataata atatcaatga cggcgcagta atcaaagtca
    28561 tcggagtcgg tggtggcggt ggaaatgctg taaaccgaat gattgaagaa aacgttaaag
    28621 gtgttgaatt cattacagcc aacacagacg tgcaagcatt aaaaaattca aaagctgaaa
    28681 cagttatcca attaggcccc aaatatactc gtggtttagg tgctggttct cagccagaag
    28741 ttggacaaaa agcggcagaa gaaagtgaac aatccttacg cgaagcttta gacggtgcag
    28801 atatgatttt catcactgca ggaatgggtg gaggaaccgg tacaggtgct gcaccaatcg
    28861 ttgcaggtat cgcgaaagaa ttgggcgcat tgactgttgg tgtcgtgaca cgtccattca
    28921 cttttgaagg accgaaacgt ggccgttttg ctgcagaagg tatcgctcgt ttgaaagaaa
    28981 atgtggatac attattaatt atctcaaata accgtttatt agaagttgta gataaaaaga
    29041 caccgatgct tgaagctttc cgtgaagcag ataatgtttt acgacaaggt gtacaaggta
    29101 tctctgattt gatcacagcg ccaggttacg ttaaccttga ctttgctgac gtgaaaacag
    29161 tgatggaaaa ccaaggtaca gcattgatgg ggatcggtgt ggctagtgga gaagatcgcg
    29221 tagtagaagc aacgaaaaaa gcaatctcct ctccattgct tgaaacatct attgatggag
    29281 ctgaacaagt acttcttaac atcacaggcg gtttggatat gacattgttc gaagcacaag
    29341 atgcttcaga tatcgtagca aatgcagcaa ctggcgatgt aaatatcatt ctaggtacat
    29401 ctatcaatga agaaatgggc gatgaaatcc gtgtgacggt catcgcaaca ggtatcgatg
    29461 aatcaaaaaa ggaacgcaag tctagccgac cagctagaca agcacaaatg caatcacctg
    29521 ctcaaaagac tgtattagat atggatcaag ctaaaccaac ttcttcagaa gaagaaaaca
    29581 gttttggcga ttgggatatc agacgtgaac aaaatgttcg tcctagagtt gatgattcca
    29641 attttgacaa tatcgaaaaa aaagaatttg atacattcaa ccgcgaagaa acaaaatctc
    29701 atggcgatga tgaattaaat acgccaccgt ttttccgtcg taaaaaataa aggagaggaa
    29761 gagcacatga ttgctgacaa cttgcagaaa atcaagcaag aaattcagga ttcgtgtgct
    29821 cttgttcatc gtgatccgaa agaagttaca ttagtagctg taacaaaatc tgttgaatcc
    29881 gctcaagctg aagaattggt tagagctggc gttatgaatt gtgcagaaaa ccgagtagat
    29941 aaattattag aaaagaaaca agatttattt gattttcctg aaataaagtg gcatttaatt
    30001 ggtaatttac agcgcagaaa ggtaaaattg attgtaaatg aaattgatta ctttcatgcg
    30061 ttggacagtt taagattagc agaagaaatc cagaagcggt tagagaaaga attatcctgt
    30121 tttattgaag taaatgtttc tggtgaggaa tcaaaacatg gaatttcacc agatgagctt
    30181 ttaccgtttg tggaatcttt agctgcctgc acaaaaatca aggttgttgg gttgatgact
    30241 atggcgccta aagaagcttc tgatgagcaa atccataaag tgtttggtac gctgaaagaa
    30301 ttgcgtgatc ttgttcaagc aaaaggttat gcccatgcac catgtagtga attaagtatg
    30361 gggatgagtc aagactttca aattgcgatt gaagaaggtg caacatttat ccgtgtagga
    30421 acagcgctat tcaaagacga ataaaggagg aacaaaaatg ccgcttttaa acagaggagc
    30481 aattgctaat tttttcggat tagctgatga ggaagagtac gaataccaag attatcctga
    30541 aacaacaaaa aaggctgtac agccaactaa aaaaaataca acacagaatc aatcaactca
    30601 ttctgtatct gctgcgaata ctcatgaaaa acaaaaaacg gcgacttcac gtcctgtaac
    30661 gcatgaaagt attcagccgg ctgaaaagaa aaaagtaatt gaactacatc aatcacctac
    30721 aaaaactgta gcgaaagaaa gaacagcttc aagaacctct acacaaccta aagcaaatct
    30781 tgctggaaaa atcactgtaa tggaaccaag agcttattct gaagctatgg cgattgctaa
    30841 acgaatcatt gctggagaag caaccttagt gaattttcat ttagtagaag aaaaacaagc
    30901 gagaagaatc gttgattttc taacaggaac agtttatgca ctagatggag atatcaaacg
    30961 tgttggagat gagatttttc tttgtacacc gactgggatg gagattgaca gttcaaccgc
    31021 tcaatctttt gcagatgcga atttgtttga tctgtaagag gaggaactac gatttatact
    31081 attgcactat tacttcattt acttcaacgg gcagcctatt tctatagcat tatcttgatg
    31141 gtatatgcat tgctctcttg gtttccaggc gggtatcaat cagggctagg acgattatta
    31201 gctaagatct gcgaaccgta tatcagtttg ttcgatcgtc tgcctttgag gattggaccc
    31261 ttggatttca caattttagg ggcaatcctg atcttgaact tagccgtaca aggtttagca
    31321 cagatcgtta tatggataac tgtccattta gtttattgat tgaagcaaca aaatctttat
    31381 tctcagaata gataggaggg gaaaattttg gatgcaaatg tataccagca ctttcgggca
    31441 gatgaacgta catttatcga ttcggtaggc gattggattg aacaggttca aggacaatat
    31501 gcaccctatc tgaccgaatt tcttgaccca agacaatcct atattttaga aacactgatt
    31561 cgacaaagca gtgatttgcg tttcatgttt tatggcggat atgaacaggc agaaagaaaa
    31621 agatgtttga ttttccccga ttattacgaa cctgtagagg aagattttga aacagcattg
    31681 tatgaaatcc attatcctag caaatttgcc agcctaagtc atgggaaaat ccttggaact
    31741 ttagtgggaa cggggatcaa acgctcttac tttggcgata tcatctcgga cggtgatcgc
    31801 tggcaagtat ttattgcaaa tgaggtggat cattttgttg ctgcacaagt aacaaagatt
    31861 ggaaaggttg ctgtacgctt ggaaccaatc aaatacacag aaatgattat gccgaaagat
    31921 agctggacac aagaaagagg aacagttagt tccatgcgac tagacagtgt gatctctgag
    31981 atttataata tttccagaca gcggtccaag cagctgatcg aagctggtaa agtaaaagtg
    32041 aactggacag aaaatacaag acctgatttt gtacttgaac tattagatat tatttcagtc
    32101 agaggttatg gcagagtcca gatccaagaa cttgagggga agacgaaaaa agaaaagtac
    32161 agggtactgt tcggtgtatt acgtaaataa aataaaaagg tggtttactc atggctttaa
    32221 ctccattaga tatccaaaat aagacattcc cgacaaaaat gcggggatat aatcaagacg
    32281 aagtagatga tttcttagat ttagttgttc gtgactatga ggaattgact caaagaaatc
    32341 gagaattaga aaaagcagtc aaacattctg aagaaaaact tgagtacttc aatgaattaa
    32401 aagatgcttt gaaccaatca atcatcgtag ctcaagatac ggctgataaa gtaaaaacaa
    32461 gtgcaagcaa ggaatccgaa gtgatcgtta cttctgcaca aaacaaagca gatgagttag
    32521 ttgcgaacgc tgaaaaacgt gcacatcaat taacaactga tgcagaagaa aaagcaagaa
    32581 aaatcttgac agatgcaaca gaaaaagcaa gacagcttgc gacagaaact gaagatctta
    32641 agaaaaaaac acgagtattc catcaacgta tcagcttgat gcttgagtct caattagaac
    32701 aagtgaaaag tccggaatgg gatgaaattt tacaaccatt ctccagctac gtgactgaca
    32761 gccatgaagt gatcaaagaa gttttagcaa aacagcttga caatgaaaat gatactgatg
    32821 taaactcaga cacaggagtc atagaagcac ctatgacggc atttgataca caggctattg
    32881 atatctcgat cgtgcctggt gaaaatgaaa acgagtagaa cagaaaagca ctttgttatt
    32941 cttaaagcga gccgatgaga gtggaagttc ggtaagacac tgcatggtga aagatcctct
    33001 acttgagacc tgcttttccg aaattagagt aagaagcagc ggctgacctc gttattggtc
    33061 acagagagtt tgcactagat agatgcaact aaatttgggt ggtaccacgg catgttcgtc
    33121 ccttgcggct gaacatgctt ttttgtgtcc ggaaaacaaa taaaggagtg tatggttgaa
    33181 tgaaaatgaa agaaaccttg caattaggaa aaacaaaatt ccctatgcgt ggtaatttgc
    33241 caaaccgaga aggcgagtgg caaaaagaat gggaagaaaa tcgaatctac caaaaaagac
    33301 aagagttgaa cgaaggaaaa cctagctttg ttcttcatga tggacctcca tatgccaatg
    33361 gtaatatcca tttaggacat tcattgaata aaatcagtaa agacattatc attcgttcaa
    33421 aatcaatgtc tggctatcgt gcaccatatg ttccaggatg ggatacacat ggattgccga
    33481 ttgaacaagt attggctaat aaaggcgtga aacgtaaaga aatgtccatg gcagaatact
    33541 tggaaaaatg tcgtgaatat gcactttccc aagtagataa gcaacgagca gatttcaaac
    33601 gcctaggagt tgcaggagat tgggaacatc cttatgttac attagatcct tcttatgaag
    33661 ctgcacaagt acgcgtattt ggtaaaatgg ctgaaaaaag ctatatttac aaaggattga
    33721 aaccaattta ctggtctccg tcaagtgagt cttctttagc ggaagctgaa attgaatata
    33781 aagatgtaaa atctccatct atttttgtag ctttcaatgt caaagacggc aaagatattc
    33841 ttgatgaaga tacagctttt gtcatttgga cgacaacacc atggacattg ccagcaaacc
    33901 aaggtattgc tgtaaatcca aaatttacgt atgtattggt ggaagccgat ggccgtaagt
    33961 ttgtcgtagc gaaagatttg ctagaaacag tccaatcaga aatcggttgg gaaaatgtaa
    34021 cagttctaaa agaatttgct ggacaagaaa tggaatatat gactgcaagt catccattct
    34081 atgaacgtga atcacttgtg attttaggcg atcatgttac tttagacgct ggtactggat
    34141 tagtccatac tgctccagga catggggaag atgactatat cgctggtagc aaatataaac
    34201 tatctgtagt gtcacctgta gactcaaaag gattgtttac agaagaagca cctggatttg
    34261 aaggtatctt ttatgacaaa gcaaatccta tgatcactga tctattaaaa gaaaaaggag
    34321 ctttgctgaa gctagatttc tttactcata gttatcctca cgattggcgg acgaaaaagc
    34381 ctgtgatcta tcgggcaact ccacaatggt ttgcatctat cgataaattc cgtcagaata
    34441 ttttggatga agtagaaaaa gtagagtggg ttatcccatg gggtaaaaca cgtttgtata
    34501 atatgatccg tgaccgcggc gattgggtaa tttcccgtca gcgtgcatgg ggagtaccac
    34561 ttccgatttt ctatgctgaa aatggagaag caatcatcac accagaaaca atcgaacatg
    34621 tggcaaaatt atttgctgaa catggatcaa ttatctggtt tgaaaaagaa gcgaaagaac
    34681 ttcttcctga aggatttacc cacccaggtt ctcctaatgg cgaatttaca aaagaaaaag
    34741 atatcatgga tgtctggttt gattctggtt cttctcatga agctgtttta cgtcagcgac
    34801 cagaattatc attcccagca gatatgtatt tagaaggatc agaccaatat cgtggctggt
    34861 tcaactcaag tatcacgact agtgtggcta tcaatggtgt agcaccatac aaatcagtta
    34921 tttctcaagg atttgcttta gatggtgaag gccgtaagat gagtaaatct ttaggaaaca
    34981 tcattgcacc agaaaaagtg atcaaacaat ttggtgcaga tatccttcgc ttatgggtat
    35041 ctagtgtaga ttatgaagca gatgttcgtg tttctatgga tatcttaagt caagtggcag
    35101 aagtatatcg taaaatcaga aatacgatgc gtttcttatt agcgaataca gaagatttcg
    35161 atcctaaaaa agatactgtt tcgtacaatg atttacgttc tgtagacaaa tatatgaccg
    35221 ttcgattgaa ccaagtaatc aaagaaatcc gagaagaagg gtacgacaaa tataacttca
    35281 tgcatattta ccgtacagtt atgaacttct tgacggttga tctatcgtca ttctacctgg
    35341 attttgctaa agatgtagtt tatattgaag cagaagatga ttatcaacgt cgttgcatgc
    35401 aaactgtttt ctatcaaaca gctgtagctt tgacaaaatt attgactcct atcattccgc
    35461 atacagcaga agaaatctgg tcattcctga aagaagaaga ggagtacgtt cagttaagcg
    35521 agtttcctgg ttatgaagag tttgcaaacc aagaagaatt gatggatacc tggactgctt
    35581 tcatggactt ccgtgataat gtcttaaaag cattggaaga agcaagaaac agcaaattga
    35641 ttggtaaatc tttggaagca aaactaactg tttatccaaa acaacaagtt cgtgaaatgt
    35701 taaaagcttt ggatgcagat attgcacaat tattgatcgt ttcaccagac tactttgaag
    35761 ttatggcagc agatgaacaa gtgccagata acgctatggt atttgatgat gttgctatta
    35821 cagtggaaaa agcagacggg gaaacttgcg atcgctgtcg ccaagttcgt aaagatgtag
    35881 gagtagatga aaaacttcct catctatgcg gtcgttgtgc aaaaatcgtt gaagacttct
    35941 atcctgaagc tgtagctgaa ggattcgaag aaaagtaaaa gggaaaaggg ggtgtgacat
    36001 gttctttgtc acaccccctt ttgttatcaa tcgtttaatt ttccacgttc acgataccag
    36061 ttatctggtt gccagatcca ttcgaatccg tctttttcaa gtagttcgaa agctgcttta
    36121 ggtcccattg atccagcttt gtagttggga aaatccggtt gtgtttggtc ccaagctttt
    36181 cgaatgacat ccacgatatg ccaagattga gcgacttctt cccaatgaga gaagttcgtt
    36241 ccatcaccat tcaacgcatc taatagtaat ttttcgtaag cttctgggct attttcaacc
    36301 atttctgcac tatttctaaa ttccaatttg attggttccg tttcaaatcc ttgccctact
    36361 tctttcccat tcaaggataa agaaaatcct tcagttggct gaatatagat cgttagaaca
    36421 ttaggtggaa gtgtcgtatc gtcacatggt tcatcgacag atgttttgaa tacattaaca
    36481 ggaacttgtt tgaatacgat gttgatccga gtcccttttt cagtcaaacg tttgcctgta
    36541 cgtacataga aaggaacacc tgaccaacgg aagttatcga tagtgaattt gcctgcgacg
    36601 aatgtttcag tctctgattg agcatccact ttgtcttctt cgcgatagcc cttgaattgt
    36661 acaccatcca attcaccttg agcatattgc ccgcgaacga agttttctaa cacttcttct
    36721 tcgttataaa tgcggatagc tttcaatgct ttgatctttt ctgtacggat ttctttttcg
    36781 gaaaatgcta ctggcggttc cattgcaagt aatgctacaa cctgtaaaat atgattttgc
    36841 accatgtctt taagtgcgcc actatgatcg tagtagcccc cgcgatcttc tacaccgagg
    36901 ttttcgccaa aagtaatttg gatattgtcg atatagcgat tgttccattg agcctcaaaa
    36961 atattattgg caaaacggat agctgagatg ttttggatca tttcttttcc taaataatga
    37021 tcaatcctga aaatatcttg ttctgggaat acttctctga tttgctggtt caattcaaaa
    37081 gcagactggt aatcagaacc gaacggtttt tctatgatca aacgattgaa gccatttttt
    37141 gtcataatat tttgtgattt caaatgagaa acgatcgttc caaaaaattg aggtgccata
    37201 gccaaataat agacacggtt accgtttaat tgatagtgtc catctaatcg gtcagacaat
    37261 tcttttaatg tctgataatg cgttgaatca tttacatcat gggattgata gtaaaaatgg
    37321 cttgcaaagg tagttgcttc ttcatctgaa ggattcaatg cacggattgt ttctttgaca
    37381 acttcacggt agtgctcatc gctccaggga cggcgagcgg ttccgattac agcaaaatgc
    37441 tcgcctaaat ttccttttct gtaaaggcgg aacaaagacg ggtacaattt acgctgggct
    37501 aggtcacctg taccgccgaa tatcgtaaac aatacatttt tttcattaga catgtggata
    37561 cactccttta tattttatcg acaaaaatcg tgctggccgc cttaaagctg acggggattt
    37621 tctttctatc attttggata gtgatcggtc cttcataagc tgcgatgtca acgatctcat
    37681 attcttcgtt gatattcagg tcaatagtga ctaagtaatc caacaattct ttttcatcaa
    37741 gcactcgtgc gatacgcact tttgtaccga caggatagga ctccaatgtt tgtcggcgtt
    37801 cttcatggac aacttcctct tttttaggaa tcgatcctcc atgaggacaa tgttcaggat
    37861 gattcaaata gttttctaaa tgatctgcaa gatgttccga agtcacatgt tccaatactt
    37921 cagcatcatc atggacttca ttccagctat agtctaaatg ttcaactaaa aaaacttccc
    37981 ataagcgatg tttgcgaatc agactgctag ctttttgcag tccgcttgca gtcaattgta
    38041 caccttgata aggggaatgt tctacaagct cttctttcac aagtttagaa atcatttcac
    38101 tgacagatgc agctgagaca tctagactag atacgatttg tttattgttg actttcgttg
    38161 tatctccacc tagttctaaa atgattttca aataatcttc tcgatttggt gtcatattcg
    38221 atcatccttt catggtaaaa aaccatcatt agtaagtgta acaaaaaaaa cgttaattga
    38281 ctatttttct aaataaaaaa caaaggattt ttagacgttt ttacaacaaa aaacgaaaga
    38341 tgatagagaa gactcattct ttcgtttata aaggatatat atatacattg ggagggatct
    38401 atttttcaga taacaactta tatgaagtgt ctgcagattc gtcataaaga gtttgttcgg
    38461 gataagaaaa gataagataa aaaccatact gatcacgtac gactaaacga tattcatcta
    38521 tttctaccac agatgtgcca agagactgac cgttgatcaa aaacgtgtaa tcagatgtaa
    38581 tctgtaattg ttgtttttgc tggctatcac taaagaacca tgtaccttga tactttgaga
    38641 gtccttctga cttttccgct tgttttttct ttttcaagta aataaaactt gagagaacgg
    38701 taatgagtga accaagaaaa tagatcattt tttttatcac caaatcactc ctttatgaga
    38761 aaataatcgc atagacatat tctttcttct ttatttcatt gtaccgaata aaagctaaaa
    38821 tttctctaag actttcgact ggttttttta ctgtttttct acaaattatg ataggataca
    38881 tagtaggaag aaaggtgaca taatttgaag tatcagcaag tggaaatagc tttttttatt
    38941 gaccgcccga atcggtttat agcacattgt atgaaaaaag acggagaaat aattattgcg
    39001 catgtcaaaa atacaggtag aggaaaagag gtcctgatac caggcgctga agtggctatt
    39061 gtatttgctc ctggacctaa acggaaaacc gcctatgatc tgattgctgt aaaaaagaag
    39121 ggtgactggt ttaatatcga tagtcaattg cctaatcggc tggcaattga tggtatttta
    39181 gacggcacga tccttttacc aggattaact ggtccattta cggtatacaa gcgtgaagta
    39241 acttatcttc actccaaatt tgatatttat ttagagacgg tggtcggaca aaaagcattt
    39301 gttgaagtca aaggtatgac acttgaaaat aaagcgattg gtgcttttcc tgatgcgccc
    39361 acactcagag gattgaaaca tataggagaa ttaacgtatg ccgcacaaga tggttatgcc
    39421 gcttatgtct tgtttattgc tcagttcgaa catcttcatc tggcaacgat ccatgaagaa
    39481 atgcaaccag cactagctga tatggttcga catgcgcagc aaagcggagt tcaaatcctc
    39541 gcctataatt gtcaagtatc ggcagatgag gtaactgtca agcaggccat tccatttgat
    39601 gtagatatcc cttttgaaga tcctatacaa tagaaagaag gtaaaataat gatcaactta
    39661 ggtattatcg gaacaaattg gattactcat caatttgtcc aagcagcttt aagcacaaaa
    39721 aaatatgaat tgacagcggt atattctaga catttggaaa aagcacagga atttggttct
    39781 cattatgaag gggatatcga attcgcaaca gatttgaaga cttttttcaa tattactcat
    39841 atggacacca tttatattgc ttctccgaat agtcttcact ttagtcaagc taagcaagca
    39901 attttggcag gaaaaaatgt gatagttgaa aagccagctt tttcgacgcc aaaagaaatg
    39961 gatgaaatca tccatttggc aaatgaacac aatgtgtatt tttttgaagc agcaagaaat
    40021 atccatgaat caggttttaa aaagattgcc gattacttac cattaaaaga cgagataatc
    40081 ggtgcaaatt tcagctatat gaaatattct tcgcgctatg accaagtatt agatggagaa
    40141 gaaccgaata tcttctcgac tcatttctca ggtggtgcat tgatggattt aggtgtttac
    40201 cttgtatatg cagctgttgc ttggtttgga atgccaaaag aagtccatta tttcccaaga
    40261 aagatttcta ctggtgtaga tggtcttgga tgggggatct tacgttatga gaattttgat
    40321 gtatcgatcc agccaggtaa aatcggtgat tcttatttac cgtcagagat ttattttgac
    40381 tcaggaacgc tagtgatgaa tggagtcaat gcaattgaac aagcacaatt tcatgatcgc
    40441 gcgcataaag aaatcattga tttggcggta acagctgcag aaaatccaat ggcagatgaa
    40501 gcagaggatt ttgccaatgt catggctcat ccgaaagatc cagcttgggg attgttgtat
    40561 gaagagtgga tcgagctagc tagaaatgtg aaccaagtga tttatgattt aagaaaaaat
    40621 ggcggcatca aattcgatgc agataatgag gaagattttt aatgaacgaa tttgtacaag
    40681 tatttcccga aaattggcag aaaaaatatg aggataaagg gtttatctca ccttctctta
    40741 ttcaacgagc agtttttcag ccactaagta ataaacaaag tatagtagcg gtttctccta
    40801 caggttcagg taaaacactg gcttatcttt ggcctctact actaaacgtg gaaccaggtg
    40861 aagcaagtgc gttggtcatt tttgcttctt ctcaagaact agctattcaa gtagcagatg
    40921 ttgcacgtga atggggaaaa gacaaagagt tgaaagtcca atctttggtg ggcggagcca
    40981 acgtcaaacg tcagattgag ggtttgaagg aaaaaccaga aatcttgatt ggtacaccag
    41041 gaagaatatt ggaattgatg aaagcaaaaa aaataaaagc acatcaagtg aaaacaatgg
    41101 tatttgatga agcagaccag ctatttgacg ctgggaacag ccaaatcatt gatcaaattc
    41161 ttcatcaagc tccaacagag tatcagctag cttttttttc agcgacagca gatcgttccc
    41221 ttgaaagcat tgaaaaaatc actgggaaaa cgtttgagac aattgacgtg acagcagagg
    41281 acgattctcg aaaaggactg agacattact ttttacgtgt accgatacga aagaaagatg
    41341 actacttgag acgtctcacg catatagaag attttcatgg tctgattttc tttaatcaac
    41401 tgactgaatt aggcgtaatg gaagaaaaat tgctgtatag aggtgtaccg gtaggaagtc
    41461 tggcctctga tcagaacaag ctactccgaa aagcagcgct agaacaattc aaaaagaaaa
    41521 tggtcagagc acttttgacc acagatgtgg cggctagagg tttagatatt acaggtcttc
    41581 cttatgtagt gaatgcagaa gttccgttaa gtgaagaagc ttatttgcat cgttcgggaa
    41641 gaactggtcg aatgggtaat gaaggaatag taattacact cgtacaagat aataatctaa
    41701 gagatttaaa acggctttta cgtccatcaa atataacact tgaagagatt tttttatacg
    41761 gaggtaagct gcaaactgag cagccaacca aagaagatgc agctcctgca gtaaataaaa
    41821 agtatgcgaa aactttttct ggaaaggagc agaacccaga atataacaaa gaaaaaataa
    41881 gaaaaaaagt taaaaataaa tcaaaaaaag agagaaataa aggagcaagg cgcaaataag
    41941 cgccttttta tgctatgatt atgaaaacgg gaacaaaatt ggttgccaga ggaggggtta
    42001 tgtgaaagaa cgtcaagaag agatcttgca gctattgaca gaaaaccaac aaggattgac
    42061 agcaagtgat gtagctgagc gcttaactat cgatcgaagc aatgcaagcc gatatttaag
    42121 tgaactatac aaggcccatc atattgtaaa aaccgctggc cgaccagtcg tttactcttt
    42181 gccaacggaa aagtcaaaat ctgatgaagt gcatgtcgac tcttctacgc aagtgacttt
    42241 tgaaacactc gttggagaaa atgattcttt aaaagtcagc atacagcagg caaaagctgc
    42301 gattctttat ccaccgcggg ggttgcatac gattattttt ggagaaactg gtacagggaa
    42361 gtcgatgttt gctgaatgta tgtatcattt tgcaatcgat tcagagatgc tttctgcaga
    42421 tgcgccattt gtttctttca attgtgcgga ttatgctcaa aatccgcaac tgctctttgg
    42481 acacatcttt gggatcaaaa aaggggctta cacaggagca gctcaagata gtcccggtct
    42541 aatagccaaa gcagacggcg gtatattgtt tttagatgag atccaccgtt taccgccgga
    42601 agggcaggaa atgctgttta ctttcattga taaaggtatt taccgtccgc ttggagaaag
    42661 tgcacaagta catgaagcat ctgttcagat cattggggcg acgactgaat cttcagaaag
    42721 tttcttatct acgttcaatc gacggattcc tatggcaatc accttgccga atcttgcttc
    42781 acgttcactg gatgagcgtt atgaaatcat ctctcttttt atcaaacaag aagcgaatcg
    42841 tttgaatcaa cgtatcgata tcgaaaaaga agcgattctt gccttcatgc tttatgatgc
    42901 ggaaggaaat atcggtcaag tgaaacgaga cttgaaacta gtctgtgcaa aatctttttt
    42961 acattaccgt actcatcatg agaaaaagct aatcattcga aaagaagaac tttcccttca
    43021 agtacaaaaa ggattactga aaatcaagga aataccggaa cgtctcgacc gattcatgga
    43081 cagcaaaagt cagtatttga catttgaacc aggatttgct gatgttgtct ggtcacaaga
    43141 tccagaacga aacatgcagg tctataacga tattgaggaa aaagtcactt ctctttccga
    43201 aaccggtgtg gagaacattg atctagagac cctcatttca aaagatgtgg atgcttattt
    43261 ccagacatat gtcaaagaat tgactaaaag ttcgattccc aaagatcttt tgccagatga
    43321 aatctggcgg ttaaccaatc aattgtatga tgtggctgaa aaacggttag accgtattta
    43381 taatgaaaaa gctcgttttg cttttgcttt gcatctgcaa agtacattgg atcgtgtgag
    43441 agaaggtcat atgatcgtcc acccagattt gaataacgta cggaaaaatt ttaagaggga
    43501 attccaggta gcaatcgatc tttcaacgat cattgaagaa gaagaacaag ttgagattcc
    43561 ttttgatgaa atcggcttta ttagtatgtt tctttctatc aatctaggag aaagtgagcc
    43621 tgttaaagaa aacaaagtag atgttctcat attgatgcac ggcagacaga ctgcttcaag
    43681 catgttggag atggcacaag aactattagg aacaaaagta gggaaagctt ttaatatgcc
    43741 actggttatg gaagttcaaa caatgtatga agaagtgtta caatatgtca aaaactatca
    43801 agagcatttg acgaatggcc tactcttgtt aacagatatg ggatcattaa acaccttcag
    43861 tgatttgatt tatgaagaga ctggcattcg gacgaaagct atcacgatga caagtaccat
    43921 gattgtgata gaagcgatac ggatggcaag tgttggaaga agcttagaag atatttatca
    43981 aaatatccag ctttcctttg aatcgatcgt acgtgatcag ttcaaagcgg aaaagtctga
    44041 aactttacag cgtaaaaaag ccgtaatcgt gacatgcttt actggtgaag gagtagctgc
    44101 caaattatat cagcgtatta tgccagtgat taatcaaaat aaggtagaac tgatccagat
    44161 gcagttttta gaacgtgaat cattcaaaaa acacatcgat gccttattgg aagaatatga
    44221 gatcaaagca attgcgggaa cagttgatgt tgattatcaa aatatcccat ttttttcagc
    44281 ctatgacgtt tttgatgatg aaaaactaaa tattttaaag cgtatagctg atgatgagat
    44341 cccgacagct aaaattgtcg attcacttgc tggatcatta agcgatgtct tttctattga
    44401 tgaactgatc cgttcattgc aaaagacagt tcagcagatc cagaccgatt tgtcgattat
    44461 cgtggaacct ggagtagata ctggaatcgt tatccattta gccttcatgt tagacggatt
    44521 gatcaaacaa gaaagccatc ggcaatttga gaaactgtct attttctcaa aaatttatcg
    44581 tttggaaatg gatatcgttc gggcaaactt gatggcacta gaacgaaaat atcaggtaaa
    44641 aattccagaa gatgatatcg catttcttac acaaatgttt cttgaaaata aaataaaaaa
    44701 atgaacacac taatcatact gtttacacac taaacatctg tgtgcgcact ataaaaaaga
    44761 gctaaaaagc atgaaaacgc tatttcagct ctttttttta ttggcatgct tcttgcgtta
    44821 tataaaagtg taaaaaaata aaaggagtgt taaaaatggc aaaaaaaaca atcatgttag
    44881 tttgttcagc agggatgagt acgagtttat tggtcacaaa aatgcaaaaa gcagcagaag
    44941 aaaaagggat ggaagccgat atttttgcgg tctctgcctc agatgcagat aataatttag
    45001 aatctaagaa tgtcgatgta ttattattag gtcctcaagt acgtttcatg aaagctcaat
    45061 ttgaacaaaa acttgcgcct aaaggtattc cattagatgt catcaatatg caagactatg
    45121 gcatgatgaa cggagaaaaa gttcttgcac aagcagaaaa tctaatgaaa taaatcaagt
    45181 atgcagttgg gggtataaca acgtggaaga tcaacaaaat ctagaagcca tcatggggtt
    45241 aatcatgttt ggcggtaatg caaagagcga tgcaatggaa gcgattgcgg ctgctaaaat
    45301 cggtgatttt gagttagcgg ataggaaaat cgctgatgca gaagaatcac ttatacaagc
    45361 gcatcatgca caaacagaca tgttgacaca agaagcacaa ggaaatcata tgcaagtgac
    45421 attattgact gttcacagtc aagatcactt aatgacatct attgcgttta ctgatttagc
    45481 aaaagaaatc atcgaacttt atcgtcgcgt atacagcgaa taaatacaac ttcttttttg
    45541 aacggaagaa gaaaaatttt tgaacggatt ttttattttg ttataaggag aactctcttt
    45601 atataaaaaa cttataggag gaagtaaaaa atgaacggac tatcaagttg gatggagaaa
    45661 tacatcctgc cggttgccgc aaaaatcgga gcgcaaaaac atttagttgc tttgcgtgat
    45721 gcttttatcg gcacactgcc cgcaacaatg gcgggatcag tcgctgtaat gatcaatgca
    45781 attattcgtg atttaccgca acagtttaac agtacctatg ctgacagttt agcagcagac
    45841 aaaggaatct ttgccgtatt gaatgtcatc atcggaatca atggttttgt ctggaacggt
    45901 acactagcaa tcgccggatt gatctttgcc ttttcttggg ggtataatct tgcccgagct
    45961 tatggagtaa atgatctagc tggggggatc gttggtttag ctaccttgat tcaaggtatt
    46021 tcctttggtc cagcattatc agcagcatta gatgtgaatg ttccttcaaa tgtcgcagat
    46081 gcaatcaata gttctgagat tggtgcaacg attgcagatg gcacactgtc agttggatta
    46141 tggggttggc taaaactaga tcatttgaat ggaaacgctt attttacagt tatgatcatg
    46201 ggagctattt cagtcattat tttttgtaaa ttgatgcttg ccaacattac gatcaaaatg
    46261 cctgattcag taccaccagc agtatctaaa gcatttgcag ccattttacc agcgacgatc
    46321 gctttatacg tcattgctat tatcaattat atcgtaggtc gtgtagctga tgggcaactg
    46381 atcattgatt tggtgcaaaa atatattgca gaaccattcc taggattatc tcaagggttg
    46441 ggagcagtat tgatcgtcac agtatttgtt caagtattct ggttctttgg tattcatgga
    46501 ccaaatgtat tggcaccagt gctagaggga atctggggac aagcacaact aatcaatatt
    46561 gatattttcc aaaaaggtta taacggaaaa actggtacac cagccgtgtt agatgcaatc
    46621 gaagatggaa aagcttatat gtgggtccgc ggatcgttcg atgcttttgc atggtttggc
    46681 ggatcaggcg gaacgatcgt cttactgata gctatcttga ttttctctaa acgtgcagat
    46741 tatctaacag tagctaaatt atccattggg cctggtatct tcaatatcaa cgaaccagtg
    46801 atgtttggtc tgccaatcgt attgaatgca atcatgttta tcccattctt gattgctcca
    46861 gtagtagcaa cagcaattgg atggacagct acttatcttg gcctagtcgc tccggtatcg
    46921 cagcaagtca catgggtagt accgccgttc ttattgtcat tcttagcaac aggagcggat
    46981 tggcgggcac cgatcgtaac attagtatgt atggtcgtga ctttcttgat ttgggcacca
    47041 tttgtcatct ctgcaaacaa aatggaccct gccgcaggcg aacaacaata agatgaacgt
    47101 gaggagcggg acgctttaag tttcgttcct cataatgaat tttcgaggag gaactgaaat
    47161 gattacaatt gccagtgcaa tgcaacaaga tgaaatcaaa gaattgttag aaggatacaa
    47221 tgaagaagga atgactttta catttaaaga aaaacaagga atcaagctgt tctttgaaac
    47281 aagtgcagaa gatccggaag ttgcggctaa aaaagcaaag gaactaatca aagcacagcc
    47341 ttggggaaca gtcctttatt ttcaatcaac agctgtttag aaaatggttt aaaaagatag
    47401 gatggttgag atgataaaaa acggtttatt tttatgtagt attggttctt tgatcgtgtt
    47461 gtatagtttg aatccacatt cgcaaaggta tgatttgtat agtatggtca caggtctttt
    47521 tctaacagga ttaggaatct atttcttcat gaaggggaaa aaaagagaag aagcaaagga
    47581 gaaaaataac taatggaaat cacgaaaaaa ttaaatattc cagcatcgtt tttttacgat
    47641 aaagttatgg actctgtcct tttcgatgta cgtaaagcga ccggcaaatc agtaactaga
    47701 aaacagctga aaaatttgga gtacgtcaaa caattctcgc aaaataaccg ggctcgcata
    47761 aaaatcgaag aagtcattga aaaccagtct tataagtttc gtacatcgac aacgaagaat
    47821 gaattcgtcg ttcattatca aatcaaacca ttggacgatt cgacctgtga ggttcattac
    47881 acagaaaaaa tggagtctta tggcatgctt caaaagttga atgatatgct tttaggaaca
    47941 atcttgatgt attttaaacg gagacgtttc aaaaaaatgc tagatatgat cgaagcaacg
    48001 tattaaatca atgaaacatg gtactattta gttattgaaa aatgaagtaa gctagaagga
    48061 atttttcata ggaagataga tctttcctgc gaatcctcac tagctttttt gtaggaaggg
    48121 gcagcaaaat gacaaacaaa gtaagagagc taggtgtatc ttcgattgag gaaatgtttg
    48181 atttagccac ttatgcattt aatggggcag atacacctga aaggcgagaa cgttttcgta
    48241 cacttgcgga gaattcttgg aattacggat tttttgatga ggaggatcgg ctaacaagtc
    48301 aagtcatggc gacccctttt ccagtgaatt tttacggaca agagtaccta atggccggca
    48361 ttggctatgt tgcttcttat ccagaagcaa ggggacaagg cgggatcaat cagatcatgg
    48421 aaaagatcct tgaagattgc cgtgatcgta aagtaagctt atcttatctg gcgccattct
    48481 cctatccctt ctatcggcgc tatggctatg aacaatcatt tgataaaatc agttatcaac
    48541 taaacagtcg tgatgtgccg tttatcaaaa aaaaatctgg aaaaataaag cgtatggatt
    48601 ttgaagatgc aaaagctgca atcagaagaa tttatgaaca gatgccggaa aaccaacgag
    48661 ctggtctgaa gcgcgaagac tggtggtata cgtataaatt caagcaaaaa ggcaattacc
    48721 agtttgcttt gcattttgat gataatgagg aagcaacggg atatatcgtt tatcagcttg
    48781 caacgtcttc ctttattatt gcggagtggg gatttttgga tcatgatgct ttttgcagcc
    48841 ttgttcgctt cgtaagttct cacaatggtg cttttgaaac atttgaatat tcatgcggct
    48901 acggcggtaa tgatcaaaat tatctattag aaaatccatt tgcttctgta atgatcacac
    48961 cttatatgat ggcgaggatc gtagatattt ctttgttttt ggaaaattat ccatttaaaa
    49021 agagacaagc tgcatttgcc ctacaaatag aagaagatca ttatgcgaaa tggaatgaag
    49081 gaatcgtgga agtaacaatc gaaaatggaa aaaatcatct ctcaaaagta gaacaaacaa
    49141 aactcccaat cgttcgagga agtatccaac atatcacgca gctattatta gggtatcgta
    49201 aagtggaaga gttggtattc caagaaagaa tacaggtgac aaaagaatat gaagaaacgt
    49261 taagctcaat cttgccacag caacagccaa ttcttaatga ctatttttga taaaaaacgg
    49321 gtgatgacaa tatttttgtc accacccttt tgtttttcat ttggcatttg aaggaaaatg
    49381 ttaaagtaaa tcctcttttt tcgtaaattt gaattttttg ttttcttttg ttttagggtt
    49441 ttcttttttg tggtcttgga ttgcttcttt cgtaatcttt tcccgatttt ttttagatga
    49501 atctaagcgt tctgcgtata ctttttcttt tggcttatca ttcattggaa aggacctccc
    49561 tacattattt cattctacct ctagtttatc atctgttaag gactagtgga aacgttttgc
    49621 ctaacttata agctgatttt tttagcagat aagcttgcaa gtaaaaaaca aacaagctac
    49681 aattgattta ttgaaattgt tcctgtagct cagtaggata gagcaatcgc ctcctaagcg
    49741 ataggtcgct ggttcaattc
//
LOCUS       DACECR010000009        49678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.009, whole genome shotgun sequence.
ACCESSION   DACECR010000009 DACECR010000000
VERSION     DACECR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.009"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            253..528
                     /locus_tag="IWO17_000461"
     CDS             253..528
                     /locus_tag="IWO17_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289360.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            541..2619
                     /locus_tag="IWO17_000462"
     CDS             541..2619
                     /locus_tag="IWO17_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0289361.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2779..4665
                     /locus_tag="IWO17_000463"
     CDS             2779..4665
                     /locus_tag="IWO17_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0289362.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4677..5624
                     /locus_tag="IWO17_000464"
     CDS             4677..5624
                     /locus_tag="IWO17_000464"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0289363.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5644..6171
                     /locus_tag="IWO17_000465"
     CDS             5644..6171
                     /locus_tag="IWO17_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0289364.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6234..6887)
                     /locus_tag="IWO17_000466"
     CDS             complement(6234..6887)
                     /locus_tag="IWO17_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0289365.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7019..7861
                     /locus_tag="IWO17_000467"
     CDS             7019..7861
                     /locus_tag="IWO17_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0289366.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8019..8915
                     /locus_tag="IWO17_000468"
     CDS             8019..8915
                     /locus_tag="IWO17_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0289367.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8918..9631
                     /locus_tag="IWO17_000469"
     CDS             8918..9631
                     /locus_tag="IWO17_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0289368.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9651..10169
                     /gene="msrA"
                     /locus_tag="IWO17_000470"
     CDS             9651..10169
                     /gene="msrA"
                     /locus_tag="IWO17_000470"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0289369.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQDYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10166..10393
                     /locus_tag="IWO17_000471"
     CDS             10166..10393
                     /locus_tag="IWO17_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0289370.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10545..11096
                     /gene="lepB"
                     /locus_tag="IWO17_000472"
     CDS             10545..11096
                     /gene="lepB"
                     /locus_tag="IWO17_000472"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0289371.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11179..11721)
                     /locus_tag="IWO17_000473"
     CDS             complement(11179..11721)
                     /locus_tag="IWO17_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0289372.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11958..12356)
                     /locus_tag="IWO17_000474"
     CDS             complement(11958..12356)
                     /locus_tag="IWO17_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0289373.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12616..13476
                     /gene="ylqF"
                     /locus_tag="IWO17_000475"
     CDS             12616..13476
                     /gene="ylqF"
                     /locus_tag="IWO17_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0289374.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13469..14236
                     /locus_tag="IWO17_000476"
     CDS             13469..14236
                     /locus_tag="IWO17_000476"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0289375.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14292..15149
                     /gene="dprA"
                     /locus_tag="IWO17_000477"
     CDS             14292..15149
                     /gene="dprA"
                     /locus_tag="IWO17_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0289376.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15271..17349
                     /gene="topA"
                     /locus_tag="IWO17_000478"
     CDS             15271..17349
                     /gene="topA"
                     /locus_tag="IWO17_000478"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0289377.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17318..18694
                     /gene="trmFO"
                     /locus_tag="IWO17_000479"
     CDS             17318..18694
                     /gene="trmFO"
                     /locus_tag="IWO17_000479"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0289378.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18892..19797
                     /gene="xerC"
                     /locus_tag="IWO17_000480"
     CDS             18892..19797
                     /gene="xerC"
                     /locus_tag="IWO17_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0289379.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19834..20382
                     /gene="hslV"
                     /locus_tag="IWO17_000481"
     CDS             19834..20382
                     /gene="hslV"
                     /locus_tag="IWO17_000481"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0289380.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20396..21796
                     /gene="hslU"
                     /locus_tag="IWO17_000482"
     CDS             20396..21796
                     /gene="hslU"
                     /locus_tag="IWO17_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0289381.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21814..22605
                     /gene="codY"
                     /locus_tag="IWO17_000483"
     CDS             21814..22605
                     /gene="codY"
                     /locus_tag="IWO17_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0289382.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22687..23562
                     /locus_tag="IWO17_000484"
     CDS             22687..23562
                     /locus_tag="IWO17_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0289383.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(23694..24332)
                     /gene="plsY"
                     /locus_tag="IWO17_000485"
     CDS             complement(23694..24332)
                     /gene="plsY"
                     /locus_tag="IWO17_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ0289384.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            24459..24893
                     /locus_tag="IWO17_000486"
     CDS             24459..24893
                     /locus_tag="IWO17_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ0289385.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25059..27104
                     /gene="parE"
                     /locus_tag="IWO17_000487"
     CDS             25059..27104
                     /gene="parE"
                     /locus_tag="IWO17_000487"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ0289386.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            27116..29566
                     /gene="parC"
                     /locus_tag="IWO17_000488"
     CDS             27116..29566
                     /gene="parC"
                     /locus_tag="IWO17_000488"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ0289387.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            29843..32074
                     /gene="pflB"
                     /locus_tag="IWO17_000489"
     CDS             29843..32074
                     /gene="pflB"
                     /locus_tag="IWO17_000489"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ0289388.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            32318..33079
                     /gene="pflA"
                     /locus_tag="IWO17_000490"
     CDS             32318..33079
                     /gene="pflA"
                     /locus_tag="IWO17_000490"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ0289389.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            33164..34090
                     /locus_tag="IWO17_000491"
     CDS             33164..34090
                     /locus_tag="IWO17_000491"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ0289390.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            34272..35165
                     /locus_tag="IWO17_000492"
     CDS             34272..35165
                     /locus_tag="IWO17_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ0289391.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            35299..35424
                     /locus_tag="IWO17_000493"
     CDS             35299..35424
                     /locus_tag="IWO17_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0289392.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(35558..36190)
                     /locus_tag="IWO17_000494"
     CDS             complement(35558..36190)
                     /locus_tag="IWO17_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289393.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(36338..36880)
                     /locus_tag="IWO17_000495"
     CDS             complement(36338..36880)
                     /locus_tag="IWO17_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ0289394.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            37015..37404
                     /locus_tag="IWO17_000496"
     CDS             37015..37404
                     /locus_tag="IWO17_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289395.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            37408..38172
                     /locus_tag="IWO17_000497"
     CDS             37408..38172
                     /locus_tag="IWO17_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0289396.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            38301..39248
                     /locus_tag="IWO17_000498"
     CDS             38301..39248
                     /locus_tag="IWO17_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0289397.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YLPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            39378..40118
                     /locus_tag="IWO17_000499"
     CDS             39378..40118
                     /locus_tag="IWO17_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289398.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(40409..41077)
                     /locus_tag="IWO17_000500"
     CDS             complement(40409..41077)
                     /locus_tag="IWO17_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0289399.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            41211..41498
                     /locus_tag="IWO17_000501"
     CDS             41211..41498
                     /locus_tag="IWO17_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289400.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            41914..43452
                     /locus_tag="IWO17_000502"
     CDS             41914..43452
                     /locus_tag="IWO17_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ0289401.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            43449..44414
                     /locus_tag="IWO17_000503"
     CDS             43449..44414
                     /locus_tag="IWO17_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0289402.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            44401..45723
                     /locus_tag="IWO17_000504"
     CDS             44401..45723
                     /locus_tag="IWO17_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0289403.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      45786..45890
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            45964..46707
                     /locus_tag="IWO17_000505"
     CDS             45964..46707
                     /locus_tag="IWO17_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0289404.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            46722..47714
                     /locus_tag="IWO17_000506"
     CDS             46722..47714
                     /locus_tag="IWO17_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0289405.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            47726..48463
                     /locus_tag="IWO17_000507"
     CDS             47726..48463
                     /locus_tag="IWO17_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0289406.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            48453..49175
                     /locus_tag="IWO17_000508"
     CDS             48453..49175
                     /locus_tag="IWO17_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0289407.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(49259..>49678)
                     /locus_tag="IWO17_000509"
     CDS             complement(49259..>49678)
                     /locus_tag="IWO17_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ0289408.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        17145 a         8507 c        10409 g        13617 t
ORIGIN      
        1 tgtcgcagcc ccattttttt gttgtgataa aagaacaaga aaggcaggaa tgggaaagta
       61 aacatccttt tgtcagaaac ttttatcttt cttaagtttt acttgatatc tgatactatt
      121 tggaatagaa tgaggcgttg aaaaatttat ggaaatttct aactagcaat aaacgatgaa
      181 acaatgaaat atttttcatc caaaaagaac tttgctataa tgatggaagt ttgtgtatag
      241 gagatctgga aaatgagaga gagttttgaa caacaaaaaa aactgttgca tgatcgctat
      301 ggcgcccttt cgatggatga ccgccgtcaa atactttgta agttgcgtaa aagaaatata
      361 ttgatgtatc gtcagttgga acgtctgaaa catgatctgc ttcgtttgga gtcaaaacgt
      421 gtacagtgtg aattagaggg gaaccaaaca caggttgaag tagtcgaaac caaaatttta
      481 aagaaaaaag aacaattttt aaaaatgctt acacaaaata aaaaataaag gggatggacg
      541 atggaatttg ctgagttagt tattgtattt gcagtaacga tcaccttatc gaacatagct
      601 agccgcattt tgccaatgat tccagctcca ctgatccaaa ttttccttgg agtggtatta
      661 ggtctgacag agtggggaca atcaatcgat tttgaacctg aattgttctt agttatgatt
      721 attgcaccat tgttatttag agaaggagaa aaagcagata tttcttcgat tctcaaaaat
      781 ttcgggacaa ttttgttctt ggcatttgga ggagtcattt tgacccttgt cggggtaggt
      841 gcgacgttat cattcctgtt gccaagtgtc ccacttgctg cctgctttgc ttttggagca
      901 gcgttaggtc cgacagatgc ggtagcagtc agttctcttt ctggacgagt gaatatccct
      961 aaaaaggcga tgcatatttt ggaaggagaa ggtcttttga atgatgcatc tggtgttact
     1021 gccttccagt ttgctcttgg tgcgctgatc accggatctt tttcagcagt aaacgcgggg
     1081 atgagtctag tagtttctag tattggggga gctttgatcg gctttttgct tgtctggttc
     1141 aaacaaaaaa taattcattt gatcgaaaaa gcatcagcac aagatgtcac agggtatctg
     1201 ctgatcgaac ttttgcttcc ttttctagcc tatgtattgg cagaggcatt tgatgtatct
     1261 ggaatcattg ctgcagtggc agcaggtatt ctccaagctt ctggttttag gaaaatctct
     1321 gtgtttgatg cagaattgtc gagcttgtcc cacagtacat ggacaactat tgcttttaca
     1381 ctgaatgcat tagtatttat atttttagga atcgaattga cgcaagtgtt ctcgccagta
     1441 tggggagacg gtttatatcc aaacggtctt ttattagcga ttatcgtatt aatctctgtc
     1501 atgctttttg tgatcagatt catttctatc agtctttttt atgtattcaa agatggcagt
     1561 aaaaagttca aaaaacagtt gaacgagatc ttgatcttaa catttggcgg tgtaaaggga
     1621 acagtcagtt tggctacgat ttttattttg ccattttcaa tcaataatat gatgttttac
     1681 cagcggtctt tgcttttgtt tctgacggcg ggtgtcatct tagtgacttt ggtcataggg
     1741 atcattgtct tgcctatgct gacggaaaca gaggaagcga aaagcacgga tttgaatgct
     1801 ttgatgattt tagaagaggt cgttgaagtt ttgcgaaaag aaataaagga aattgatcgt
     1861 aatacaaaag aatttttagc cactgaagca gtgatagaaa attatcaaga acggatacgt
     1921 gatctgtact tggaagattt aaccgatgat gaaaagcaag aagtacaaga aatacaagca
     1981 ctgattttgt cgatcgaacg ggatggattg gatgaaagtt atcgttcggg caagctttct
     2041 tctaatggtt atcgttttta ctcccgtttt ctttctcgat tcgaacattc tatcacaagc
     2101 cagattttgt cttttatcgg cttttggttc attgttgtcc gtaggttaat gcgaatcgtt
     2161 ctccatccga aaatgttttg gcaaagaaga tatgctgatc gtcagacatt tattagtgat
     2221 gaagatatcc aagaaatcag agagacatat caaaaaaata ctcagttgat tattgaaagt
     2281 ttagataact tgactgatat ttatgatgaa acgatggttc aattttttat ccagcaacgc
     2341 aaacttgaag gaataaaaat gaactcgggg aatttgattt cttcgtggat gatccagcaa
     2401 gatagtctgt ttacgaaaaa aatgctaaga ggttattatc ttgaaagaaa aatcattgat
     2461 gaatacgaag ttgctgaaaa aatcacgacg ttttctgcga acagctatcg caggaatatc
     2521 aatttgttag aatcttatac gatgaacaaa ccatctgata acttttcttt ccgatttgcg
     2581 tttcgagcaa agtcaaaaaa aacgagtcca ataaaataaa aaggtaaaaa ttttcaagtt
     2641 ttataaaaaa caaaacatat cagaacagga tcgaatcggt cctgttcttt ttttgagaac
     2701 acaaaaagaa caaataccaa aaaaattttc ctgaagttca ctatttttat gatacaatat
     2761 ccaagataag gagggaacat gttgaaagaa ttaaaagtaa ctgacctcaa aaaaacttat
     2821 ggtgaaaaag atttgtttga ccagatttct tttttggtcc acgaaaaaga ccgtatcggg
     2881 ttgattggga cgaacgggac gggtaaaaca agtctcttaa atattattgc cggtatcgat
     2941 agcagcgacg gtgatcggca aacagtgttt tatccaactg attaccgtat tggttatctt
     3001 tctcaagcag ctgaattttc cgatgaatta actgttctcc aagcagtctt tcaaggaaac
     3061 agccccttga tccaaactgt tcgagcatat gaggaagcat tgatcgaact aggggaaaac
     3121 ggagaagacc ctgatgtgca aaaacgttat gctaaagcag aagaacaaat gaataaagaa
     3181 gatgcttgga ctacagatac caacgcaaag attattttgc aaaaattagg aatcagtgaa
     3241 ttagataaaa aaatcagtac tttatcaggt ggtcagaaaa aacgagttag tctggcgcaa
     3301 gtgttgattg atgaacccga tttactatta ctagacgaac caacaaacca tttagattat
     3361 gaagcgatcg agtggttgga aaactatttg aaacaatacc gtggcgcact attgatggtt
     3421 acccacgatc gttattttct agatagagta gcaaaccgaa tcttcgaact ttcatttgga
     3481 aaattatacg agtataaagg aaattatgaa gcttacgtct tagaaaaagc agaacgtgat
     3541 cgtgtagaag tagaacaaga agaaaagcgc aagcggcttt ataaacagga attagcttgg
     3601 atgagggctg gcgtacaggc acgtggcacc aagcagcaag cacggatcaa ccgtttcgaa
     3661 gatctaaagg agaacttgta ccaagtaaac caagaagatg atttagagct gaacctagcg
     3721 acacaacgtt tagggaaaaa agtactggaa atcaaagatg gcagttaccg tataaatgac
     3781 caaacactgc tagaacattt agatttattg attcaatctc gggaaagact aggtattact
     3841 ggtaaaaatg gtgcgggaaa atcgacattg cttaatattc tagctggcag aattcccctg
     3901 gatagcggta caatgtctat cggtgaaacg gttcatcttg cgtattatac acaagaaaat
     3961 gaagaaatgg caccagataa acggatgatt gcttacttac aagaagctgc tgaagaagca
     4021 aaaacagcag atggcagtca aatcggagta gcagaattac ttgaacgatt tttatttcca
     4081 agatttatgc atgggacact gataggcaaa ctttcaggcg gagaaaaacg ccggttattt
     4141 ttgttgaaac tgctgattca gcaacctaat gttttattat tggatgaacc gaccaatgat
     4201 ttggacatag caacattgac gattttggaa gattatttta gaagttttcc aggtgctgtg
     4261 atcactgttt cccatgaccg ttatttcttg gacaaggtag cagataagtt attagtattc
     4321 caaggaaacg gaaaacaaga actttattac ggaaacatgt ccagttattt actgaagcaa
     4381 aaagagaccc agcagcctgc tgaaaaagcg aaaccaaaaa tacagagcaa ggaacctgct
     4441 gaaaagaaga aattatctta catggaacaa aaagaatggg aaacgattga agatgagatt
     4501 gcagaacttg aagaaaaaat ctcattgctc caagaagaga tgaatcatca aggagataat
     4561 ttcactcgat tacaagaatt gcaaaacgac gtatctgaaa cagaagcaca acttgaagaa
     4621 aaaatggcac ggtgggaata cttgagtgaa tgggtagaag attaggagga aaaaggatgg
     4681 aacaagcgta tcttgactta ggaagaaaat tgttggaaga agggcatcgt aaagaagaca
     4741 gaacaggcac aggcacatta agcttattcg gataccaaat gcggtttgat ctgcaaaaag
     4801 ggtttccttt gttaacaacg aaacgagtac cttttggatt gatcaaaagc gaattgcttt
     4861 ggtttttaaa aggagatacg aatatccgct atctattaca aaataaaaac catatttggg
     4921 atgaatgggc atttgaacgc tacatcaaaa gtccagacta taatggacca gatatgactg
     4981 attttggcca tagatgtttg acagatcctg aattcaatga agaatataaa aagcagagca
     5041 agcttttttg cgagaagatc ctaaccgatg attcctttgc tgaaacatac ggcgatcttg
     5101 gacacatcta cggctatcaa tggcgtcact gggaaacaaa agacggtggc tttatcgatc
     5161 aaatcaaaca agtgatcgaa gcaatcaaaa agactcctga ttctagaaga ttgatcgttt
     5221 ctgcatggaa tccggaagat gttcctagca tggctttgcc tccttgtcat acgatgtttc
     5281 aattttatgt tcaagaggga cggctaagct gccaattgta tcaacgcagt ggggatgtgt
     5341 ttctaggcgt tccgttcaac attgcaagtt atgcgttgct gactcaccta attgcacatg
     5401 aaacaggttt agcggttggc gaatttgttc acacattagg agatgctcat ctttatatca
     5461 atcatatcga acaaatgaaa gaacaattga gccgagaaat acgcgcattt ccgactttag
     5521 ttttgaatca ggaaaagcag tcagtatttg attttgagat ggaagatatc caattagaag
     5581 gatatgaccc acatccggcg atcaaagctc ctatcgctgt ttagaatcag aaaggatgag
     5641 aaaatgttag ctgcaatttg ggcacaggat gaaaacggat tgattggaaa agaagaccgt
     5701 cttccttggc gtttgccaaa tgatctgaaa ttcttcaaac aaatgacaga agcaaataca
     5761 ctagtgatgg gacgaaaaac attcgaaggg atgggtagcc gtccgttgcc gaatcgtcag
     5821 acgattgttt tgacgagaga ttcttcctat aaagcagaag gcgtgcatgt catgcatagt
     5881 gtggaagacg tgcttgctct ggaaaaagaa acagacggta tctttttcat cgctggaggg
     5941 tcagctgttt atcaagaatt tctgccattt tgtacgatat tataccgtac agtcatccat
     6001 catgtattcg atggagatgc ttattttcca cctgttgact ggtctgattg gtcactgatc
     6061 aatactagtc aaggagaaat ggatgaaaaa aatcgttatc cgcatcaatt tgaaacgtat
     6121 cagcgtaatg agaatcctgt aaaaagcggg acaggcgaat tgacgatata aaaaagctat
     6181 ggatagacta gctgttattt agagctgtcc catccatagc tttatttttt ttattaagta
     6241 aagaataaaa cagagaagta cataaaacct gcacctaaca taacaaacaa atgccaaaca
     6301 acatgcatat agcggataga attcaagcta taaaagaatg cgcctatcgt gtaagaaagc
     6361 ccgcctgcta ccatcaaaat aacaccagtc attccaagag agtgataaag aggaacagca
     6421 gccacgacac agagccaccc cataaaaata tagatgatcg tagatacttt tgatactctc
     6481 tcccgtttat gcaacgtcaa cgctttatat ataatgccaa gaatcgcaaa caaccagatt
     6541 aatacaaata aagtccaacc tagccatcct cggatactca gcaaacaaaa aggagtataa
     6601 cttcctgcaa tcaaaagaaa aatcgaacta tgatcgaata cttggaacac cttttttgct
     6661 tttgtaaaaa tcaatgaatg aaacaaagta gacgttaaaa ataaaagaat catcatcgat
     6721 ccatagatag cataagagac aatatggagg gaggaatgca agtgggcacc ttttactaaa
     6781 agaaccacta gtcccgcgat actcaatcca gtcccaattc catgggtgac tgcattaaac
     6841 acttcattta atatttcata cgttcgatta aatttcgttt tttccatgaa aacggctcct
     6901 ttactaggtg taattaatga aaaatcatta tttgtctgtt atactaaaaa tagaaattgt
     6961 cttacatgta gtttaacatg aataaggcat cttgttaaag aaagagagag agaaaataat
     7021 gacaaatgta aaaatcgtaa cagattcttc atgtacgatg gaaaaaaatg tacgcgatgc
     7081 tttaggcatt cgtgtcatgc cgctatccat catgatcgac ggaacagtat atcccgatga
     7141 tgaccaacta gacggcgaaa gatttatgga aatgatggcg aaagcaaaaa atctgcccaa
     7201 aacaagccaa ccaccaattg gagaattcat ctcgatgtat gatgagctag gagaagacgg
     7261 cagtgaagtg atttcgatcc atatgacaaa aggcttgagt ggaacagtag aagcagcaag
     7321 acaagccagt aatctaacaa aaacgaaagt aacggttatc gacagtgata ccactgacca
     7381 aggattatct tttcaggtaa ttcgagcagc tgaattagca aaagaaggta aaacagtaga
     7441 gaaaatcctt gaagaagtca aaaaagtaca agacaataca aaattataca tcggaatatc
     7501 cacactagat aacttggtaa aaggtggaag aatcagccga acaactggtt tgctgtctag
     7561 tgttttgaac atacgcgtgg tgatgaattt cgcccattct gaattaatcc ctgtaactaa
     7621 ggggcgagga aagaaaacgt tcgataaatg gtttaataat ttgaaaaatg aactgaaaga
     7681 attgccaaat gtgcgacaaa tcggtatttc acatgcggat gcaaaagaat tggcgcaaga
     7741 attcaaagag ggtttgcaag aattattccc ggacatgcat atccctgtac tgcatacgaa
     7801 tccggttatc gctacgcata ctggtaaaga cgcgtttgca atcatgtatt atgtagatta
     7861 gtcgaaaaga ggataagccc tcagcctgaa aggcttgcag cttattctct ttttctttat
     7921 aaaattagga aaataattgt tttctttttt cataaagact acctttttga taaaattttt
     7981 gataacatta gaaccaatcg aatacaagag gtgaaaaaat gaaattaacg aaacgacatt
     8041 tgatcctcgt tttgttccca ttattaattg cgatcatcat ctatgcaggt ttagctgctt
     8101 ctataccgaa agctggccca ctaatgaaac cagtaactac tgtgagagaa gatccaaata
     8161 aaaaagaaaa acttcactac atagctatcg gagattcgct gactgaagga gtaggagatc
     8221 aaaccaagca aggaggattc gttccccttg tcgcaaatga tctaaaagac cgttataatt
     8281 tgaccgcggt tgaaacagaa aactatggtg taaatggtga acgaagtgac cagatcttaa
     8341 agcgagtaaa aacaaaagaa gaaattcgaa acaatatcaa aacagctgat tttatcacac
     8401 tgactgttgg cggtaatgat ttgatgaaag ttatccaaaa taatttattc gggttaacgg
     8461 tcaattcctt taaaaagcca ctgaaaaact atcaggaaaa tgtcacagaa ctattggaag
     8521 aatttcgatc cctcaataaa accgcaccta tttacatttt aggcatctac aatccatttt
     8581 atttgaactt tcctgagatc actgacatgc agacaattgt taacaattgg aacaaaggaa
     8641 cagaaacggt gacaaaaaaa atgccgaact gctactttat cccaatcaat gatttgcttt
     8701 atcaaggatt gaatcaggaa gtaggtgtca cagaagaagg aacagatcaa acaactagca
     8761 cgacagaaaa tctaaatggc atcaaaaata atgtgctcta tgaagaagat catttccatc
     8821 caaataattt aggttatcaa ttgatggcaa atgcagtgag agatcagctg atcgagacac
     8881 aaaaccaatg gttagagaag gaggatgatc aataaacatg aaaacaagat ctgacgtatc
     8941 ttcaaacaaa acaaagaaaa caaattactg gaaattcgcc ttccttatcc ttcttggggt
     9001 agtacttggt tcgaccgtat ttttaggcag ccgtattttt gctaatcggg aaccagaact
     9061 cccagaaata ccagctctta cggaaagaca aggagatcct gtcttaacga tcaattcaaa
     9121 taaagaaaaa gtcaatcaaa tcatcagctt tttccttagt gagtatcaaa aagatagtga
     9181 tattgaatat aaattttatt tggaaaatga agcactactc aatggtactt tcgaagtact
     9241 aggttttcct atcgatttct atttatattt tgatccgtat gtgatggaaa acggaaatgt
     9301 ccaactaaaa gctagaagtc tttctatcgg aacattgagt ttgccaatca aagatgtgat
     9361 gaatatgatc aagagaaact ataaattacc agattggatc gaagtcaata cagatgatct
     9421 gactatcatg cttaggttgg atcaattccg aatgcaaaac gggatgtata ttaaagcaga
     9481 taaaatcgac ttagtaaatg atgacattcg gtttagtcta tatttgccaa aagatacctc
     9541 tgatgcttca aacaactcaa ataccaacaa aactagcacc agcagtactg aaagcaagac
     9601 aaaagaaaat aaaaaatcat cggatgagta gtattagagg gggaatcata atggaacgag
     9661 caatatttgc aggaggctgc ttctggtgta tgatccagcc tttcgacacg cttcctggta
     9721 tccatacagt gatgtcagga tatactggag ggcatgtacc agatccgact tatgaacaag
     9781 taaaaagcca aacgactgga catacagaag cagtggagat tatctatgat ccaacattga
     9841 tttcatatga acaattagta gaactttact ggcagcaaac tgatccaact gacgctttcg
     9901 gacaattcga ggatcgcggt gacaattatc gcccggtgat cttttatttg acagaagaac
     9961 agcggatcat tgctgaaaaa agtaagaaac agttagctga aagtggacgt ttcgaacagc
    10021 caatcgttac aaaaatcgaa ccagcccaac cattttatct agcagaagaa gaccatcaag
    10081 actattataa aaagcatcct gaaaatttta aaagaaacca tgcacgccgg gctgccttta
    10141 tcgaagaaaa ttggaggaac gaagggtgaa tcgaagcttt tttcattatt taatgacact
    10201 gcgaggcggt ggcacacatg atgctttatc tgttttcgct acagaagccg gtaaagacat
    10261 ccaatttcct aagcacagct ctagctacga agaaatttct aattatctgg aactgaatgt
    10321 cgactatttg ccgtccatgg atatctttga cgaagcatgg gaaaaatatt tagaaaataa
    10381 tcatgacata taagagatag ttttgtactt tatgatcaaa aaagtttttt gtccaatagc
    10441 ctaattgaaa gaattatttt tatttcagct atctagtcgc atttttttga tatttttctt
    10501 ataataaaga aagaatgaaa aaaagaggga tggattgata gagattgaca aaaaaacaac
    10561 gctatattaa tcgcttctgg cttgtattca aatacttact tgtttctgta ttccttgcgt
    10621 tcatgttgcg gggattttta ttcatcccgg taccagtgga aggcaattca atggaaaacg
    10681 tgttgaagca aggtgatatg gttgtgatgg aaaaattttc ggagatcaga cgatttgata
    10741 ttgttgtttt tcaattagca gatggcacga tttatatcaa acgagtaatc ggtcttccag
    10801 gagaaaatgt cagctaccaa aatgatcaat tgaagatcaa tgggaaagta gttaaggaac
    10861 cttatctaac taaaaacctg aaatcagatc atgcaaatgc atcctatacg acggacttta
    10921 cactacaaga gctaacggga caatccaagc ttccggaaga taattatttt gtacttggag
    10981 ataatcgaag agtttcaaaa gatagtcgtt cgtttggcac gatcaataaa actgatattc
    11041 taggtaaagc aagatttgtc tattatccac ttgatgaaat aaaatggatc cagtgagaaa
    11101 caataaagcg tgcagtctgt tgagactgca cgctttattg tttaatccaa aatcagttta
    11161 ctaccttagt acgtaaaatc aaacagctcg tttaaacact tttctagcct ctaaagtttt
    11221 gataaatgaa taaacaacca ttatttgaat agggatttgg atcatattcc tgatgaatct
    11281 acctagaaaa attactttga aatctgcacc aaacaaaaca gacatcagcc aagaattgat
    11341 aaaaaggctg actgggatca taatcaataa ttgggcagcg atgacggaag gcaggtttat
    11401 ctttttatga tataaaaata agccattaat aaatccacca aggaaagccg tgataagaaa
    11461 agcagcttga aacgttcctg aaccaaatat cgtgaaattt aaagtgttcg acaacatttc
    11521 aagcaaacca gcccagatcg gtccatatag ataacccata agcgccatac ttaaaaatcc
    11581 gaatgtgatt tgaaaataag caggaccaat cgctatcttg tataagatga catcaagggc
    11641 taataaaaga gcagaggtcg acaacaatct tgcattcatt tcgggcaatc taaaaacgtc
    11701 ttttgcaatc aaagtgttca aaatattctc tcctttgtca gttaataata aaaggatacc
    11761 ataaaatcac caatgtgtca tgacacattg gtgatttaca tacactggca tctaacaatt
    11821 aaaaaaaggg tgagaagact gcttttataa actgtaaaaa agagattgta cgaataaaaa
    11881 aaatagaggc ggagaatact tatcgtatca tccgcctttg tttttttgtt ataaaaaacg
    11941 tcagattagt tatctgctta catcttcatc catacgggtt ctaatgtatt tccgtctaga
    12001 tcttggactt ctaatgaaaa catttcttct tcaggcattc ccatatctac ttcgtggaaa
    12061 gtaccaccat tttcttttgc aatttgagca aattttttta cttcatccgc attttcaaca
    12121 ctaaacgaaa tcaaagcacc acttgttttt ttattatcag ctatttcttt atcttttata
    12181 aattttttat aaaaatcatg agagagaagc attatccaaa aagtgtcatc ccataccatt
    12241 gaaccagctt cttcagtaga aaattcttca ttttttttaa aacccagctt ttcataaaaa
    12301 gcaacggaac gattgacatc agcaacagga aaattaacaa aaaccatact attcatttgt
    12361 ttcgtctcct ttctgttttg ttcgtctatc atctcttatt taggaaacgc tgtcaaaaat
    12421 ttagattcat atatagaaac caaaataaaa atttgcatca tcaaaaaagt gaagatgtga
    12481 tagaatgagg actggttcac tttcacttaa ctaatagatg gaatttcttt ataataaaaa
    12541 agtgaattta tattgaaaaa gaaaaaaaca agtaaagccg gggaatattc cggtatgaaa
    12601 ggaatggaga gaacaatgac catccaatgg tttccgggac atatggcaaa agcccgacga
    12661 gaagtttctg aaaaaatgaa atatgtggac atcgtatttg aattggtaga cgcacgatta
    12721 cctttatctt ctcgtaatcc aatgctagat cagatcattc agcaaaaacc tcgccttgtt
    12781 ttattgaata aagcggatct ggcagatcca caacagacaa agctgtggca agaatatttt
    12841 cgtaaacaag gccatcttgc attagctatc acggcacaag aaagtaaagg aattaaaggt
    12901 ctagtacctg cggctaaaga agcattgaaa gaaaaacggg caagagatgc tgctcggggg
    12961 atcaagcctc gcgctattcg ggcaatggtc ttaggtattc caaatgttgg gaaatctacg
    13021 ttgatgaatc gactagtagg caagaaaatt gcacagacag gaaataagcc tggcgtaaca
    13081 aaaggtcagc agtggcttcg tttaggtaat gagttggaat tgttagacac accagggatt
    13141 ttatggccca aatttgaaga tgaagaaatc ggcaaaaaac tagcgctgac aggcgcgatc
    13201 aaagaccagc ttgtccattt agatgattta gcgatttacg gattagactt ttttgcccga
    13261 tattattcag gaagaatcag cgatcgttat ggtttatcaa tcgagcaaga agaaaaattg
    13321 gcaccggaat tactgatgga tattacagca aaacgtgggt ataaagaaga ctatgatcgt
    13381 ggcagcgaat tagtcgtgca tgatattcgt caaggaaaac ttggacgata cacactggat
    13441 cgttgcgaag aaatggagca aaaaagtgat gactgaatcc atccaaagta tcaaagaacg
    13501 attgaaaagc atacagagaa tagatgatcc tacactagaa tcattaagaa atgatccacg
    13561 caaaggcgtg cagcaagcac tggcttcttt tgaacgacga ttgaaaaagg aagcattaat
    13621 taaggaaaaa tatgaagaaa tgttttgctt tgaaaagcaa gggtacgcac aaggtcacca
    13681 atggattgcc ggcattgatg aagtaggaag aggaccatta gctggacctg tcgtggcagc
    13741 tgcagttatc ttgccgaaaa atgagatcat attagggctg aatgactcca aacaattatc
    13801 agagaaaaag cgagaacttc tttatcaaca agttcaagaa aaagccctgg caatcgggat
    13861 cgggatcgtt gatcagcaga caatcgatga tgtgaatatt tatgaagcaa gtaaattagc
    13921 gatgatccaa gcagtagaaa aattgtccat tgtaccaagc tatttgttga tcgatgcgat
    13981 gaccttggat ttgcctattc agcaggaaaa aataatcaaa ggggatgcac gatctgtgtc
    14041 gattgctgct gcaagtatca ttgctaaagt atatcgagac caattgatga aagaatatga
    14101 cgtgctttat cctggatatg gtttctcgca taatgcaggt tatggaacaa aggaacactt
    14161 agaaggactt gaacgacaag gaattacgcc aatccatcgt aaaagttttg cacctgtaaa
    14221 aaaatattta caataaaaaa cagccgcttt tacgtatctt attagaaaga gagacgaaag
    14281 gcggttttat gatgtatcaa atagaagaaa atttattgaa acttgcttat acaaaaggaa
    14341 tcagtagtca aggaaaatgg gcacttgcta actggatgat gcagtatcct tataaagaaa
    14401 tcggatttga agacatcatt aagataggta aaataaccaa acaccgggag ctgtttcgca
    14461 aaagttggca ggaaattcac gctaactggg gcaaaatcca atcgaaacag tcgtttatca
    14521 cttgctttga tcccaaatat ccccctcagc tgcttcactt gacctatccg ccaattgtac
    14581 tgttttataa tggtgatcta tctttacttt cttgtaacat gttaagcgtt gttggtggaa
    14641 gacacacttc tgctttagcc aaaaaaacag tttattattt actaaaacca gtgatagaag
    14701 ctggatatgt gatcgtcagt ggctgcgcga aagggataga tacatacggt caccaagcag
    14761 caatctctca tggtggacgg acaatcgcag tcattggaac agggatagag caagtctatc
    14821 ctgctgaaaa tcgtgacttg caagaggtga ttgcaaatga tcatcttctt ttgagtgaat
    14881 atcctcctca atccggcccg aaaaagcatt gttttccaat gagaaatcgg attatcgcag
    14941 ctttgtctaa aggaacatgt gtctttgaag ccaagaaaaa aagcggctct ctgataacag
    15001 cacagcaagc attggatctt ggaagaacag ttttttctgt tccaggaaat attttaacag
    15061 aacattctac cggatgtcat catttgatcc aagacggtgc agtttgcacg gtttcgggtg
    15121 aagatatctt ggcagaattt aaagaataac accatacttt tcatttgctt ttcttgacaa
    15181 acgttttctg aatggatatg attagctacg atttgtatga atcctctttt aatagtagga
    15241 acacgacaat ggaaaggagt ccattcagta atggcgtata aatatttagt gattgtagaa
    15301 tcacctgcaa aagccaagac aattgagaaa tatcttggac gaaactataa agtaatggcc
    15361 agtgttggcc atataagaga tttaccgaaa agtaaaatgg gtatcgattt tgaaaacaat
    15421 tatgaacccc attatatttc tatacgcgga aaaggcgatg tcatcaaaag cctgaaagcc
    15481 gcagcaaaaa aagctcaaaa agtttacttg gcaagtgacc cggatagaga aggagaagcg
    15541 attgcttggc atttagcgta cctacttggg ttggatctga aagaaaaaaa tcgggtggtc
    15601 ttcaatgaaa tcacaaaaga cgcagtcaaa gcagctttta aggaaccaag aacgatcgat
    15661 gtagatttag tagatgcaca gcaagctcgt cgtaccttag acagaatcgt tggttattcg
    15721 atcagtccta ttctctggcg taaggtcaag aaagggttaa gtgcaggacg tgtccaatct
    15781 gtcgctttaa aaattattat tgaccgtgaa aaagagatcc gagaatttgt tccagaagaa
    15841 tattggagca tcgacggtaa ttttaaaaaa gctcgcaaga aattcaaagc aaatttctgg
    15901 ggaatcgacg gtaagaaaaa gaaattacca gatgcacaaa gtgtaaaaga agtcactgct
    15961 agaattgatg gaacagactt cgaagttaaa aaagtagaaa agaaagagcg caaaagaaac
    16021 cctgcgaatc ctttcactac aagtagcctg caacaagaag ctgcccgtaa gttaaacttt
    16081 cgaacaagaa aaaccatgat ggtagcacag caattatatg aaggaatcac tttgggtaaa
    16141 caaggaacag tcggcttgat tacatacatg cgtactgatt ctacccggat tgccgataca
    16201 gcaaaagcag aggccgcagc atttattgaa gatacgtatg gaaaagaatt ttctagacat
    16261 gatcaccgaa agatcaaaaa tgctcaaggc gcacaagatg cccatgaagc tgtgcgtcca
    16321 acgagtgtgc ttcgtacacc agatgaactc aaacaatatt tagataaaga tcaattgaaa
    16381 ctttatacat tgatctggtc acgttttgtg gctagtcaga tgacaccagc tattttagat
    16441 acaatgaaag tgacattaga acaaaatggt gtcatcttca ttgcgaacgg ttcaaaaatc
    16501 aaattcaaag gattcatgca agtgtatgtg gaaggtagag atgacggcaa agaagagaaa
    16561 gaaaatattc ttccagaact tgaagaaggc gatgtagtcc aatctgttga tattgaacca
    16621 aaacaacatt ttactcaacc accagcacgt tttagtgagg ctacgttgat tcgtgcacta
    16681 gaagaaaacg gggtagggcg accttccact tatgcaccaa cacttgatac gatccaaaga
    16741 cgctactatg taaaattgac acaaaaaaga tttgagccga ctgaactagg agaaattgtc
    16801 aattcattaa tctgtgaatt cttccctcaa atcgtcgata tccatttcac agcagaaatg
    16861 gaaggagatt tggataaaat tgaagaaggc acggaagcgt gggtcaaagt ggttgatcgt
    16921 ttctataaac cgtttgaaaa agaactgacg aatgcggaag aaaaaatcga aaaaattcaa
    16981 atcaaagatg aaccagcagg attcgattgt gatgtctgtg gacatccgat ggtgataaaa
    17041 ttagggaaat atgggaagtt ttatgcttgc agtaattttc ctgattgtcg gaatacgaaa
    17101 ccaatcgtaa aagaaatcgg tgtgacttgc ccagtatgtc atgaaggaca agtcattgaa
    17161 cgaaaatcta agaaaaacag actattctat ggttgttccc gtttcccaga atgtgaattc
    17221 acttcttggg ataaaccagt gggcagaaat tgtccaaaat gtgacggata tctggtcgag
    17281 aagaaagtca aaggcggtaa acaagtagtc tgtgtaaatg gagattacga agaagacatc
    17341 caaaaataat agaaaagagg ttctttcatt gaaaatggta aatgttgttg gagcagggct
    17401 tgccggaagt gaagctgctt ggcagatcgc acaagcagga gtccctgtcc gtttatatga
    17461 aatgcgtcca gtcaaatcga ctgaggcgca tcacacaagt aattttgctg aactagtatg
    17521 ttccaattca ttaagaggaa acagtttggc taatgcagta gggttattga aagaagaaat
    17581 gcgccgattg aattcggtag taatcacttc ggcagatgaa acagcagtgc cagctggtgg
    17641 tgccttagct gttgaccgcg attctttttc tgaaacaatt acccgaaaag tcaaagagca
    17701 tccattagta acagtgataa acgaagaaat tactgaaata ccggaaggaa tcaccgtagt
    17761 agcaacagga cctttgactt ctcatccatt agcagaaagc atcaaagcat tcaatggttc
    17821 agatggtttt tatttctatg atgcagctgc accaatcgta gacaaagcta cgatcgatat
    17881 ggataaagta tatttgaagt cccgctatga caaaggagaa gcagcttact tgaactgtcc
    17941 gatgacagaa gaggaattca aagctttcca tgaggcacta atcaatgccg aagtggcaga
    18001 acttaaatct tttgagaaag aaaaatattt tgaaggatgt atgccaattg aagtaatggc
    18061 acagcgagga ttcaaaacga tgcttttcgg acctatgaaa ccagttggat tggaagatcc
    18121 taaaactggc aaacgtcctt atgctgttat ccagctgcgt caagacaatg ctgcagcttc
    18181 tttatacaac atcgtgggct ttcagactca cttgaaatgg ggcgagcaaa aacgtgtttt
    18241 ccggatgatt cctgggttag aaaatgctga attcgttcgc tatggtgtga tgcaccgaaa
    18301 cagctttatg aattcgccag aattgctgaa accgacttac caatcaacga aacgcgaaga
    18361 tctgttcttt gcaggtcaga tgactggtgt agaaggctat gttgaaagtg cagcgagcgg
    18421 actacttgct ggaatcaacg ccgcacgctt agcaaaagaa atggaaccag tcgagtttcc
    18481 acaagaaaca gctattggaa gtatggctta ctacattact catgcggaag gaaaacattt
    18541 ccaaccgatg aatgccaact atggtctgtt tcctgaactg ccagagcgca ttcgagacaa
    18601 aaaagcaaga tatgaagcaa ttgcacaacg agctttaaaa gctaatgagg caataaaaac
    18661 agaattagaa aaaactgcaa tcccaaatga gtaaggaaac tccatgaaga gggtgagacg
    18721 gatagtctcg ctctttttct tgcgtttttc ttaagaattt ctcaaacaaa atgtgaggaa
    18781 ttgcgccaat tgtgaacaaa cttagaaata ttggttaaat tctgacaatt tatttgtctt
    18841 tttataaacg ctgtgctaaa ttattctctg ggacaaaagg aaggtgagaa aatgacagaa
    18901 gccacgtggg aacaaaaatt tttgcgttat ttgattgtcg aaagaggata ttcagaaaaa
    18961 acgaaagaag catatgaaga agacattctc catttcaaac attttttgga ggaatctgga
    19021 gacgctgatc tgctgaaggc ggaatatttt acggttcgtg tctatttgag tgcattagct
    19081 gatcatcgtc ctagctatag cagaaattct atcagtcgca agatagccag cctacgttcg
    19141 ttttatcagt atcttctcaa agaagaagtg ataaaagaaa atccattttc ttacgttcat
    19201 ctcaaaaaga aaaatttgag actgccacgt tttttttatg aaaatgaaat gcaagcactt
    19261 tttgacagcg tagcaggaga tagtcctttg gatttgagga accgcgcttt actggaagtc
    19321 ctttacgggt caggcatccg tttaagtgag tgtagcagtt tgtcggtatc agacattgat
    19381 tttgattcag aggtcatgtt gatccatggt aaagggaaca aagagagata tgctcctcta
    19441 ggttcttttg ctcaagatgc tctgcaagaa tatttcaggg aaggccgcca ggtattgatg
    19501 acaaaatatc acgaagagca tgatatcgtt tttgtgaatc atcatggaaa accaatcacg
    19561 cctactggaa ttgaatatgt cttgaatcaa atgatcaaaa aaagcagttt ggatagtgat
    19621 atccatcctc acatgctgcg ccatacattt gctactcatt tattaaataa tggggcagat
    19681 ttgcggacag tccaagaatt gctaggacat gccaatttat cgactaccca gatctatgcc
    19741 catgtaacaa aagaaagttt acaaaaaaac tatcgttctt tccatccaag agcgtaaaaa
    19801 taaggaaaag aacaaattta ggaggaaaca aacatggtcg aatcaccatt tcattcaacc
    19861 actatctgtg ccgtcgaaaa agacgggaaa tttgctatgg ctggagatgg ccaagtaaca
    19921 atgggtgaat ctgttgtcat gaaaggcact gcaaagaaag tacgacgtat ctacaatgga
    19981 gaagttgttg taggtttcgc cggaagtgtt gcagatgcgt ttactttaga agaaaaattt
    20041 gaaggcaaac taaacgaata taacgggaat ctacaacgcg cagctgtcga attagctcaa
    20101 gaatggcgga cacagcaatc tatgcaaaaa cttgaagcga tgctgatcgt aatgaatgat
    20161 aaagaaatgc ttcttgtttc aggtacagga gaagttatca caccagatga tggtatctta
    20221 gctatcggat caggagggaa ttttgcgttg gctgcagccc gagcaatgaa aaactatgct
    20281 caaaacgaca tgagtgcgaa agaaattgca gaaaatgcgt taaatattgc cgcagatatc
    20341 tgtatcttta ctaatcataa tattatcgta gaagaactgt aagaggtgag tagatatgta
    20401 tgaattaaaa caaacaccaa gacaaatcgt aagtgaatta gatgaatata tcgtaggaca
    20461 gcacgcagca aaaaaatctg ttgccgttgc tttgcgtaac cgctatcgcc gtttgcagtt
    20521 agatgaaaaa atgcaacaag atattacacc aaaaaatatg ctgatgatcg gacctacagg
    20581 ggtcggtaaa acagaaatcg cacgacgttt ggcaaaaatc gtcaatgcac catttgtcaa
    20641 agtcgaagca acaaaattta ctgaagtcgg ttatgtcgga agagatgtag aatcaatggt
    20701 tcgggattta gtagaaaatg cgatccaaat cgtagaaaaa gagcagtaca gccgtgtata
    20761 cacaagtgct aaaaaaaatg cagaaaaacg tttggtaaaa atcttggtac ctggtatcaa
    20821 aaaagaacag aaacaatcca ctaatcaatt tgaacaaatg atgaatcttt ttaatgctag
    20881 tcagcaaaat accgctgaac cgcaagaaga agtgacagaa gatattcgaa tcaatcgccg
    20941 tacagtcttg gaacaactac aaaagggttt actggatagt cgtgaagtaa ccattgaagt
    21001 tgaagaccaa aagaaacctg caccaatgat gaacaatgga ttggagcaaa tggggatcga
    21061 cctaaatgaa actctaggtg ctttgacacc gaagaaaaaa gtggaacgga cggtaacagt
    21121 caaagaagct ttagagttat tgatcaagga agaatcagca aaattagtca aggatgctga
    21181 tatccatagt gaggcaatca aattagcgga agaaagtgga attattttta tcgatgaaat
    21241 cgataagatc acttctaaat cacagcaatc tggagaagta tccagagaag gcgtccaaag
    21301 agacatattg ccaattgtcg aaggttcaca agtaaacaca aaatacggga tcatccaaac
    21361 agatcacgtc ctattcattg cttcaggagc tttccatttg tctaaaccaa gtgatctaat
    21421 tccagaactt caaggacgct tcccgatacg ggtagaatta gatgatttga cagctgaaga
    21481 ttttgtgaag attctgaccg aaccaaataa tgctttagta aaacaatacg tcgcgttgat
    21541 cggtacagaa aatgtgtcag ttacgtttac aaaagaagca attgaacgga tcgctcaaat
    21601 cgctttcgat gtcaaccgtg acacagataa tatcggcgca cgcagactgc ataccatttt
    21661 ggaaaaacta ctagaagatc tattatttga agcaccagat atgcaaatgg gagaaataac
    21721 gatcacagaa gcatatgtgg atgacaaact tggctcaatt gcggcaaatg aagatttaag
    21781 tcgctatatt ttataaaaaa tcggaggacg acaatgacag acttgctaac gaaaacccgc
    21841 aagataaaca cacttttgca gcaaaagaat acttttgaca tgcaagcaga tttgccttac
    21901 gataaaatgg ctgtcacact aggtggtatt cttgacagca acacctatat cataggaagt
    21961 gacggagtat tactaggtta taatgaacgt cacgatgtga acaatgaacg aatcaaaaca
    22021 atgtttgttg agaaacaatt tcctgaatct tatactgata cagtggcaca gttgatgaaa
    22081 actgaagcta atatcccaat tactagtgat attactgcat ttccggtaga gtcaagagag
    22141 aaatatccat ttggtctgac gactgtcgtt ccgatctttg gagctggcga acgtttgggt
    22201 acgattatct tatctcgtat ggaacaatcg tttgcagatg acgatctagt cttagcagaa
    22261 tatggagcta cggttgtcgg tatgcagatc ctttatcaga agtctcgaag catcgaagca
    22321 gatgtacgga gtgcaacggc tgtccaaatg gcaatcaaca cattatctta tagcgaattg
    22381 aaggctgtac aagctatctt tgaagcattg gatggagatg aaggtcgtct gactgcttct
    22441 aatattgccg atagtatcgg catcactcgt tctgtgatcg tcaatgcttt acgaaaattg
    22501 gaatcagctg gaatcattga atctcgttcg ttaggcatga agggaactta tttaaaagta
    22561 ttgaattccc gttttaaaga cgagttagca aaacacagtt attagaagta aggggactgg
    22621 ggcagataga aatgtttgtt ccagccttcc cattttaaaa agtaagtcga agttggagga
    22681 atggcaatga ctgttattat tcaaaatgac cagctgatcg ccgaaatcgc tgaacacggt
    22741 gcggaattga tcagtctgaa atcaaaagaa catggtttag agtatatctg gcaaggtgat
    22801 ccagcgtatt ggggcagaca tgcaccggtt ttattccctt ttgttggacg tttaaaagat
    22861 gatcagtatg tttatcaagg gaaaacatat caaatggggc aacacggatt tgctcgggat
    22921 atggattttg aagtcattga acatggacaa gaaatggcta gtttcatctt aaggagtacg
    22981 ccggagacat tagaaaagta tccatttgat tttgaattag tgatcagcta tgaattagga
    23041 ggagacggca tcacggttca ttatcaagtg gcaaataccg gtgaaaaaga aatgttcttt
    23101 tctatcggtg gacatcctgc tttcaatgta cctcttgaag aaggattgtc atttgaagat
    23161 ttctatctgg cattttctcc attgaaatca agattacgcc taccattgaa aggcccattc
    23221 atcgatatgg atcaaaagac gttaggtcag acaaatacga atctggcttt gaatcgtgaa
    23281 ctttttgagc aagatgcctt ggtttttgaa acaaggggtc taaatgcgta cgccattcga
    23341 agtgaaaaat cttcccatag cgtaacttta agctataaag atatgcctta tgtagggatc
    23401 tggtctcctt atccaaaaga agcgcctttc gtttgtatcg aaccgtggtg cggaatcgct
    23461 gacacggtga acagtagtgg aaacttgatg gaaaaagtag ggatcaatca actaggtgcc
    23521 catgaaatct tcaagacaaa atattccatc acagtgaagt aagaggttgt gaccgagaag
    23581 ttagcaggga acaccgttta ccaaggtgtg taaaaaagct atcctgtaag taataaggga
    23641 gtgtgacatc gatttgtcac actcccgttt tatctctttg gaatgaaaaa gtatcaggat
    23701 tttttcttgc tgctattcaa accaaacggg attcgacttt cagtatggtt tctgatacgc
    23761 ttgatattgt ctttatgccg ataaaagata aaggaagaaa ctaaaaaggc aatgatcgtc
    23821 agtaaccaat tgggcttagc caaaatcaca ggagctacat aaggaaggac gatcgtagat
    23881 aaagtgatca atactgcact gatcatactg ctcaaactca ccatacttgt acaaaataaa
    23941 ctaacgataa agatgataca agaataaaca aaaaatacag gattaaatcc taaaagcatt
    24001 cctgcacttg tcgcaacagc ttttcctcct ttgaacccag caaaaatcgg aaatgtatgg
    24061 ccaagaatgg cgcatacccc aaaccaaaga gggttaatcc cagaaaggtg gaagatcatc
    24121 ggcaaacaag tagccaatgt accttttaaa atatccataa gcaggacaac aatcccggct
    24181 gttttgccta atacacgaaa tgtattggtc gttccagtat taccgctccc gtgttgtcgg
    24241 atatcttttt tgaaaaacaa tttgccaatc cagacaccgg aaggaataga acctaaaaga
    24301 tatgctaaaa taagtaataa gatcaatttc attttttacc ctctttcaag ttgaaaacta
    24361 tcctttattt tagcatgaat aggtaacagg aacaattgaa aaaatgaaag atacaagtat
    24421 aatagatgga ataaaaagaa aagcaagggg tggcagaaat gtctgtaaaa aatccaacac
    24481 aagaaacgat tttcaactat ttggaagaag caaaaaatat tgccgtcgta ggcttgagca
    24541 ataaaccaga acgaacaagt tacatggttg cacaggtatt gcaagacaat ggatataaaa
    24601 tcataccagt aaatccatta ttggctggtg aagaaattct aggcgagaaa gtctatgcga
    24661 atttgacaga aatcgatgat ccaatcgata tcgtagatgt atttagacgg agtgaatttt
    24721 tacctgaaat agcaggtgat tttttgaaaa ctgatgctaa agtgttctgg gcacaattag
    24781 ggattgaaaa caaagaagca gctgagatgc taagaaaagc aggacgtaca gatatcgtaa
    24841 tggatcgttg tattaagatt gaacttggta agttgaaaga aaaccaaaaa taaaaatgac
    24901 cacaaagaag agatgatcga tagaggggaa tgtcgaaaaa gcggttagga gataattaaa
    24961 attgatttct cgtctaccct cttttttttc acgacctttt ctagtatgat aatcgtggtg
    25021 cggttttgcc gcaaagtatt attgaaggag cgtttgcctt ggcaaaaaaa gtaaacaacg
    25081 aatacaatga ctcgtccatc caagtattgg aaggattaga ggcagttaga aaaagaccag
    25141 ggatgtacat tggatccact gacagccgag gcttgcatca tttagtctat gaaatcgtcg
    25201 ataatgcagt tgacgaagca ttatcaggtt atggcagtga gattgatgtg accatccatg
    25261 aagacaatag tataactgtt gcagatagcg gacgtgggat gcctgtagga atgcatgctt
    25321 ccggaatacc aacagttgaa gtgattttta ctgtgctgca tgctggcgga aagtttggtc
    25381 aaggagggta caaaacttct ggtggactgc acggtgtagg agccagtgtg gtaaatgcct
    25441 tatcgaaatg gctaattgta acgatcgtta gagatggcgt agagtatcag cagaaattta
    25501 aaaacggcgg taaacctgac ggtactttga aaaagatagg aaaaacaaaa aaagcaaacg
    25561 ggacgactgt ccatttttta ccagatgata caatattcag tacaacgaaa ttttcatatg
    25621 aaattttagc cgaacggtta agagaatctg cctttcttct aaaaggcgtt aaaatctctc
    25681 tttctgatct tcgtggagaa gaaccagtca aggaaatttt tcattacgaa gaaggaatca
    25741 aagaatttgt tgattatctg aatgaagaaa aagatacatt gacaccagtt gtttattttt
    25801 caggagaaaa agaaggtatc gaagtagagg tcgcttatca atacaatgac ggttattcag
    25861 aaaatgtttt atcttttgtc aataacgttc ggacaaaaga tgggggaacc catgaagctg
    25921 gtatgaaagc ggcgatgacg aaatcttata atgaatatgc gagaaaagtt ggtttgctga
    25981 aagaacggga caagaatctg gaaggtagtg atttcagaga agggttagct gctgtacttt
    26041 ctatccgtgt acctgaaaat cttctgcaat ttgaagggca aacaaaagaa aaacttggta
    26101 cgccagttgc tcgtaccgta gtagataacg tcatcagtga acaaatgggc ttttatttgc
    26161 aggaaaatag cgaaatgagc cagatgttgg tccgtaaagc aatcaaagcc agagaagcga
    26221 gagaagcagc acgtaaagcc agagaagaaa gccgaaatgg gaagaagcgt aaaaaaggcg
    26281 aatctcttct ttcaggaaaa ttgacacccg cacaatcccg aaaccctaag aaaaatgaat
    26341 tgtatttagt cgaaggggat tctgctggcg gatctgctaa acaaggaaga gacagaaagt
    26401 tccaagcaat tctaccttta cgaggaaaag tcatcaatac cgaaaaagct aaaatgcaag
    26461 atatcttgaa aaatgaagaa atcaatacga tgatctatac gattggtgca ggagtcggtc
    26521 ctgagttttc tatcgaagac tgtaattacg acaaagtaat catcatgacc gatgcggata
    26581 cagacggcgc ccatattcaa gtattactgc ttactttctt ttatcgttat atgaaaccgc
    26641 tgattgaagc tgggaaagta tatatcgcgt taccgccttt atataaagta tccaaaggac
    26701 aaggaaagaa gcaagtcatt gaatatgcgt ggacagatga cgaattggca gcaatgatta
    26761 aaaaagtcgg caaaggatat atgctgcagc gatataaagg acttggagag atgaatgcgg
    26821 aacaattgtg ggaaacaacg atggatccga cttcccgtac gttgatccgc gtccgtatcg
    26881 atgatgctgc gcaagctgaa cgtcgagtaa ccacactgat gggtgacaaa gtagaacctc
    26941 gaagaaaatg gatcgaaaat cacgtgcaat ttacgctcga agaagatggc agtatcttag
    27001 ataaaaaaga ggatactgaa atctctcctt cagtttctaa tgatttatta gatgaagaac
    27061 gagcagataa aaatgaaaac aatcagttat ttgaagtaga ataggaggtc acctgatgga
    27121 aaatcgacaa gaagtacaag aattaacttt agaagaagtg atgggtgacc gcttcggacg
    27181 ttattccaaa tatattatcc aagaacgtgc actgcctgat atccgtgatg gattgaagcc
    27241 agtgcaacgc cggatattat ttgccatgaa caaagatggc aatacgtatg acaaaggatt
    27301 ccgtaaatca gcaaaatctg tcggaaatat catggggaat tatcaccctc atggtgaccg
    27361 cagtatttat gaagcgatgg tccgcatgag ccaagactgg aagttgcggg aagtactgat
    27421 cgaaatgcac gggaataacg ggagtatgga cggtgatcca cctgctgcga tgcgttatac
    27481 tgaagccaga ttatccaagt taagtggaga gatgctagca gatatcgaga aagaaacagt
    27541 cgatctcgtg tggaattttg atgatacaga aaaagaacca acagtccttc cagctcgata
    27601 cccaaacctt ctcgtcaatg gatcgacagg tatatctgct ggatatgcaa cagaaatccc
    27661 gactcataac ttagctgaag tgatcgatgg cacaatctat atgatcgatc atccacaagc
    27721 gagccttgaa aaattgatgg aatatattcc tggacctgac tttccaacag gagggatctt
    27781 gcaaggaaaa gatgaaatca aaaaagctta tgaaacagga cgaggaaaag ttattcttcg
    27841 ttcgaaaaca aagatagagg caatgaaggg taataaacag caaatcgtga tcacagaaat
    27901 cccatatgaa gtaaataaag cgaccttggt gaaaaaaatg gatgagatcc gtttgaataa
    27961 gaaaatcgat ggaatagctg aagtacgaga tgaaagtgat agaacaggtc tgcaaattgt
    28021 cgtcgaattg aaaaaagacg ccaatgctca aggaattttg aattatttgt tcaaaaacac
    28081 tgagctacag atcaattaca atttcaatat ggtcgctatc gaccatatga ctccgcatca
    28141 agttggactg aaagacattt taagaagtta tatcgagcat cgaaagcaag tcataacaaa
    28201 acgtagtcaa ttcgatttag ccaaagctca aaaaagacag catatcgtag aaggattgat
    28261 gaaagcttta tcgattctgg atgaagtaat cgcaacgatt cgtgaaagta aagacaaaaa
    28321 agacgccaaa aagaatttag tcgacgtctt tcaatttaca gaagaacaag cagaagcaat
    28381 tgtcacattg caattatacc gtttgacgaa cacggatatc acagagcttc aaaaggaatc
    28441 cgaaagcttg attgcgcaaa tcacagagtt gaataaaatc ctttcaaatg acaaagagct
    28501 tttctctgtt atgaaaaaag aattgcgaga agtgaaaaaa aattattctt ccgctcgtct
    28561 aacgacaatc gaagacgaaa tcgaagaaat caaaattgat acgcaagtct tagtggcaca
    28621 agaagacgta attgtcagtg tgacgcgaga aggctatgtg aagcgaacaa gccttcgttc
    28681 ttatagtgct tccaaaccag aagaaatcgg gatgcgagaa ggcgactact tgctatactc
    28741 aggtgaattg agtacattgg accatgttct tctgatcaca aataaagcaa atgtgatcta
    28801 ccgccctgtc catgaactac ccgatttgaa atggaaagat gctggtgagc acatttctca
    28861 aacaatctcc aatctgtctg tagacgaatc gattttagct gttttcccat atcaaaaaat
    28921 tcaagcagag aaaacatttg tattcatcag caagaatgga ttgatcaagc aaacacgaat
    28981 gacagagttc gaaccatggc gcacctataa gagccgtccg ctcagctgta tgaagctaaa
    29041 ggcggaagaa gatgaactga ttgctgtgta tctgaaagaa gatcagccag gcttagatgt
    29101 tttcttagta acgcatcaag gaatgggttt gcgctatcct ttaacagatg ttcccgttgt
    29161 tggagcaaag gcagctggag tgaagtctat gaatttgaaa gatggagata ggattatcaa
    29221 cgggctgctt gtgttagcag aaggtgatac gccgatcgta atcgttactc agcgaggagc
    29281 tgttaaacgc atgcttgctc aagaaatcag ccaaacttca cgtgcgaagc ggggagtgac
    29341 ggtcatgcgt gagctgaaaa aaaatccgca ccgtattatc tatatgtcgg aaggaagatc
    29401 aaaagaaata accttgatga atcaaaaagg gcagcagtta acgattgacc caactgattt
    29461 ccctataggt gatcgaacat cgaatggatc atttgcgatg gatgagaaaa aaggtggaga
    29521 agtcatcgaa gttatcgatg ctcctgagat tttcatcgag tcgtaactgt tgtagtattt
    29581 taatgaagag acgccatctg tattaataca ggtggcgtct ctttttcatt catttttaat
    29641 aaatccttat acgtttgcaa aaaaagatga aagtgttcac aatgcttaaa tgttggtatg
    29701 tcaacgttga tttaaatata tgtaaataaa acataaaaat tgttaaataa aaaacaagaa
    29761 aacccttgca aagatagggt gctgtgttat actaatcacg taaaaactgt tacataaaac
    29821 agaaatttag gaggtccata caatggagca atggaatgga tttaaaggta ccaaatggaa
    29881 agaaggcgta gacactagag actttattca aaataactac actgaatatc gcggtgatga
    29941 cagctttttg gaaggcccaa cagaagcaac agaaaaatta tgggatgaat tgcaaaagtt
    30001 aaatgatatc caattcgagc gtaacggtgt ctatgatatg gacacagaaa tcgtatcaac
    30061 tattacttca cacgatgcag gctatttgga taaagatttg gaaaaaatcg tcggtctaca
    30121 gacagacaaa cctttgaaac aagcattcat gccgttcggc ggaatcaata tggctaacaa
    30181 tgcgctaaaa agcaatggct atacaccaaa cgatgaattg acacatattt tcactgactg
    30241 gcgcaaaaca cataatcaag gtgtatttga cgcttatact gctgaaatgc gtgctgctcg
    30301 taaaaacaaa atcattacag ggcttccaga cgcttacggt cgcggacgta tcatcggtga
    30361 ctatcgtcgt gtggcattgt atggaatcga ctatttgatg gaacaaaaga aaaaagatca
    30421 tgatatgact ggttatcata caatgagtga agaagtgatt cgtttacgtg aagaaatcac
    30481 tgaacaatat cgtgcattag cgcaaatgaa agaaatggct gcgaaatatg gctatgatat
    30541 ttctaaacca gctgcaaatg caaaagaagc tgtccaatgg ctatacttcg gatatttagc
    30601 agcaatcaaa tcacaaaatg gtgctgcgat gtcaatcgga cgtatctcag cattccttga
    30661 tatctatatc caacgtgatt tggataatgg agtaatcaca gaaaaagaag cacaagaatt
    30721 gatcgaccac ttagtaatga aattacgtat ggtcaaattc gcgcgtacac ctgaatataa
    30781 ccaattattc tcaggattcc ctatctgggc tactttatcc attgcaggta tgggattaga
    30841 tggtcgttca ttagttacaa agaatgattt ccgtctgctt catacattaa caaatatggg
    30901 accttcacca gaaccaaact tgactgtttt gtattctgaa caccttccag aaggattcag
    30961 aacatatgct agcagaatcg ctatcgaaag ttcttcgatt caattcgaaa atgataatca
    31021 gcttcgtgaa caatggggtt ctgatgattg tgcgattgct tgctgtgtat caggaacagt
    31081 tgtcggaaaa gacatgcaat tctttggcgc tcgtgctaac ttggctaaaa cactattata
    31141 tgccatcaat ggcggagtcg atgaagtgac tggcgcccaa gttggaccag aatatcgtcc
    31201 aatcacatca gaaatactag actttgaaga agtgaaacat aacttcatga atatgatgga
    31261 ttggttagca gaactatatg tcaatacgtt gaacatcatt cattatatgc atgataaata
    31321 ttcttatgaa gcagctcagc tggctttgat ggattctgac ttgcaacgca catttgctac
    31381 aggtattgct gggatctctc atgcggcaga cagcttgtca gcgatcaaat atgcaaaagt
    31441 aaaagcaatc cgtaatgaaa aaggaatagc agttgatttt gaagtggatg gcgacttccc
    31501 taaatacgga aatgatgatg accgtgcaga tgaattagtt tcatggatct tggaatactt
    31561 catgggacaa atcaaacgtc ataaaactta ccgcaactca aaaccaacaa cttcattatt
    31621 gacgattact tcaaatgttg tttacggtaa aaatacagga aatactcctg atggccgtga
    31681 agctggaaaa ccacttgcgc caggagcaaa tccaagttac aatgcggaac aaagtgggtt
    31741 attagctagc ctgaactcaa cagctaaaat gaattacggc tgtgcattgg atggaatttc
    31801 aaatacccaa acaatcaatc cttcaggtct tggaaaagaa caagatacaa gaatcgaaaa
    31861 cttgcgtaat gtcttagatg gatacttctc aaaaggagct tatcatttga atgtcaatgt
    31921 attcagtaat gatttattgc gtgatgcaat ggaaaatcca atgaaatatc cgaacttgac
    31981 gatccgggta tctggttatg cagttaaatt ccgtgacttg acaagagaac aacaattaga
    32041 tgtcttgatg agaacttcac acgacagaat gtaaaaagtt cgtgaaaaaa gtttttcgac
    32101 ttagcgtcga aaggacgttg aatagccatc gcatcaaaat cgaataatca gtatgcttat
    32161 ttgaaagaaa cgatttacag gggcaggact tgtatgttcc gtccctgtaa attttacaca
    32221 taacgaagcg gcaattgcga aaaattttgt tcagaaagct tcgcttcgac agctaacttg
    32281 tacttaaaat gaaatcaaag aaaagatgtg aacttaaatg gaagaaaaaa caatcggcta
    32341 tgttcattct atcgaaactt tcggttcagt agacggacct ggactgcgct ttgtcgtgtt
    32401 tatgcaagga tgccgcatgc gttgccaatt ttgtcacaat ccagatactt ggaatattgg
    32461 cggagggaaa gaatacacag cagatgaact tttagataaa gcagaacgct tcagacctta
    32521 ctggggagat aaaggtggta ttactgttag tggtggagaa cctctattgc agatcgattt
    32581 tttgatcgaa ctatttaaaa aagcaaaaga gcggaagatg catactacat tggatacatg
    32641 cggaaaacct tttacgtatg aagaaccttt cttttcacgt tttcaagaat tgatgaagta
    32701 tacagattta ctgttatttg acatcaaaca catcgataat gaagagcata aaaaactaac
    32761 gcattgggac aatgaaaata ttttagaaat ggctcaatat ttgtcaaaaa tcaacaaacc
    32821 ggtttggatt cgccatgtac tagttcctga gcgaagtgat tatgatgaat atttgatccg
    32881 actagacaac tttattcaaa ccttgtcaaa tgtagacaga gtagagattc ttccttatca
    32941 tacgatggga aaatacaagt gggaaacact tggtttgaaa tatccattag aagggatcga
    33001 accacctaca aaagaacgtg tcgaaaatgc taaacgattg cttcatacag aagagtatac
    33061 aaaatattta acgcgatagg tcgcatcgga aagaagataa gtattgcttc tttccgtttt
    33121 tttgctatta ttaaatagga gaaatcacaa aggagcgaat gacatgtcaa aaattttagt
    33181 ttttggtcac caaaatccag atacagatgc aatcggggca gccattgcgt tttcacactt
    33241 gcaaaatgaa ttaggtaaag aaacagaagc agttgctcta ggtacaccta atgaagaaac
    33301 acaatacgca ttagaccact ttcagctatc tgcaccacga gtagtagagt cagctaaagc
    33361 agaaaatgca gatgtcatgc ttgtggatca taatgaattc caacaaagta tttcagatat
    33421 cagcgaagta aatattttag ctgtagtaga ccaccatcgt attgcaaact ttgaaacagc
    33481 taatccattg tactatcgtg cagaaccagt tggatgtaca agtacgatta ttttaaaact
    33541 atataaagaa aaccaagtaa caattcctaa agaaatcgca ggaatcatgc tctcagctat
    33601 catttcagat acattgttgt tcaaatcacc aacatgtacg cctgaagatg aagcagctgc
    33661 aaaagaactt gcagcaatcg ctgaagtcga tctaaacact tatggacttg aaatgttgaa
    33721 agcaggaaca aatctaagtg ataaatcagc tgcggttctt cttgatttgg atgcaaaaag
    33781 tttcccaatg ggcagttcca acgttcgtat cgctcaagtc aatacagtgg accttaatga
    33841 agtgatggat cgacaagcag agctagaagc agcaatggaa gatgaaatga aagcaaatgg
    33901 ctacgatctg ttcgtattag ttgtcacaaa catcttagac agtgattcag aattacttgt
    33961 tgctggtaaa ggaaaagaaa aagtagaaga agctttccaa acaactttga ccaacaaccg
    34021 tgctttgctt aaaggtgtcg tttcacgtaa aaaacaagtc gtacctcaat tgacggctgt
    34081 atttagctaa tcaaatggta gtatgaagca ttcaaaatga atggataagc gattgatata
    34141 aaaagaaaca tagaacatac taaaggccga agaaatggag gagaataaca gtcaattatg
    34201 actcttgttc ttcctccttt ttacgtaaaa gccttttaag taaaataaaa cggatagaaa
    34261 agagaacata tatgaactat tattcaaaaa ataaggaaaa cttttatcaa aaactgaccg
    34321 atgatccgct tttttcttca ttgaccgatt atctatatga acatcgagaa caagaaacga
    34381 ttctaagaga attgaaaaaa gaattttcgc aaaataaatt ttcccatttt cttgatttat
    34441 tgatcgatgc aggattaatc aagagggaag aacgaagata tcatttgaac tttccaatat
    34501 ttgatcctaa agattacttg caacaagcca cttcggctgc tgaaaccata gctgaacaat
    34561 tgaagagact ctctgttgat gaacaaaaat tagcgatggg agaagttatt tgggcgtatt
    34621 gtttcgaaga tgaacggaaa gaagcgtatt tttatggagt aagaaacagc cgagagacag
    34681 aattgctgag gacaactgca ggaaatgaga agtaccgttt tatcacactt tcctctatag
    34741 aacacttccc gcttactttg gctaattact tttttgtaca gaagaatcag cttcctgtaa
    34801 cgaaggcatt caaagaatta gccgaactta ttggagacgt aaatgaatcg tacttttttg
    34861 atcaaataga agttatcgtt gacaggatac gtaagaataa atataaaaat cgccggccta
    34921 gtattttcca tcagtctcta cttgtgacga atactataaa ggaagaagaa tcattcacat
    34981 tagaattacc tattgtcgaa aaaaacaatt ttgagattga attaccaaca ttggatcctt
    35041 cattgacaat ggaggaaatg gcctttctta aacgacaaat cttttctgag ctgagcaaga
    35101 aatttatacc gcatgcattt tcttatatca aagaatatgg aacagtttta gtgagtaaaa
    35161 cctaagagag cagtaagcaa aaattatact gactcttgca ggaggagcct ttctcttcct
    35221 ttatggttaa aagaattatt tgagtatttt gcctatctac cacctattag attcactaca
    35281 tggtctggaa acaaaggaat gcaaaaaaat ggtgagaaat gtggaatgac aaaggaagta
    35341 gtgataagaa aagttagatt tttaaataat caatattatg attcagtcaa gtatggcatt
    35401 ttatgggaag aactagctga ttagattagg aagtccattt gataatccag aatcactaag
    35461 aaacgaatgg cgagtcatta ataatttatc caatcttggg ttattctttt agattgaagt
    35521 gtttaaaact aaaaaagcat ttatgaagtc gtaatagtca ggcttcaaaa tgctttttat
    35581 tttgtttgtt ttttctttgc cacataaata cacctagact gacgatgaag agcatatcca
    35641 acagatttcc tgcgacttgg atcgtcgaag ggttggtcag tggcattaaa gcaaggacaa
    35701 aaaaggaaaa caagctgtaa ataaaacagg tgacaggaac taatcgaaag aagagcggac
    35761 atttcaattg gctaaaaatg agtgaaaaaa taaaataagt aaaaatggat aaaatcacga
    35821 ctaaaccatt catgataaag aagaaaagcg tattgtgatt aaacgaataa aaaagaattg
    35881 ctaaaaaaga aaacgtttta aatgcaatca atacgcttga agcttcgttt aacgtaccag
    35941 gatgtcgatg aaaaaaatag gcaccgaaat aaacagaaaa cagtaagccg caaagagaag
    36001 acaaaatcaa aaaaaacgga ttttcttcgg cttctgtgat atcagaatga tccaaaagca
    36061 accagcctaa tgcactagaa ggcgcatgtg aggtcttaag gtatgaataa catagaaaaa
    36121 ataagatcgt caaagttgca aaaaaagagc ctaaatgtaa aaaattagtg gttctacgtt
    36181 gaatttccat aatatctcca aattaaattg ataagtatat aaagtataat aacaaattga
    36241 tgagtatatg tcgatgtttt ttaatcaaaa taataaataa aaactgtcta acatttaaaa
    36301 tttaatttag atattagaca gtttttaagt ttgtgagtta ttttccttgt cgttttctca
    36361 cttttttagt ccagaaacca cctgaaatat attttggctc ataagaaatg ataaaagcac
    36421 gctcttcgat ttctttgatc aggcgataca gttcacgttc gctttttcta ggagaaagta
    36481 tttcgagaac tagccgctca ccttccagcc ccattgcaga agtctgtgtc acaccataac
    36541 cgtgttctct cagtataccc ggcaaacttt tatcttctgc gctatttgct ggcaaaatcg
    36601 ttgtgaccat gatgtatcct aatgccaatt tgtcttcaat caaaattccg acactgatac
    36661 ctactgcgta acctaacgca tagacaaata aattcagtgg attatctaaa cgatctaaaa
    36721 caagggataa gccgacgata taaatcgtga tctcgaccat actgacaagt ggagcaatca
    36781 gtcgatagcc tttcattgtc aacatgaaac gtaacgtgtt cagagtgata taagcaaaat
    36841 taatgaggaa gatcatcccc aacatttttg gatcaatcat ttgtaaaact cctttcattt
    36901 atataacaac aatatcaaaa aaaatcgtaa gtgaagaaaa attgaaactt tatacataca
    36961 aaaaaatgat aaaatgatta taatagaatt ataaagatag gttaggtgat taaaatgaaa
    37021 acggtcaatc gtgtgttttg tgccagccac gtgctaagtc tccttctcag tatatactta
    37081 ggtgtctatt cgattcaaca gccaattgag gtagagaaca ttgtttttca gatgtcgctg
    37141 acagatggtt taattttaag cgtttttttc tttttgatac tgtttattgg taatattttc
    37201 ggtagcgttg ccagtttttt gaatttttct atttatccac tcctttcgct agcattagga
    37261 gtggtgggac tttgttctct gtgcttattt cccgagccat tcagtcctgc atttatatta
    37321 tttggtacac tgaacctgtt ccagacaact gttggagcat ggttgctatg gcgatcagga
    37381 aatttaatga aaataggtga gtaacaattg aatccaatta tattgaaacg ttctgtccat
    37441 catattccag tgttagaagt tgttctgaaa gaattaagag aggaacgtat tcccgttgtc
    37501 atctattatc acggatggca gacaagtaag gagttagttt tgacgcaagg aagaaaaatt
    37561 gcgcagcagg gcattcgtgt gatcctgcca gatgcgatga atcatgggga gcgcaaacag
    37621 ccggtttctt ctattccatc ctatacattt tgggatagta tctacggaaa tctgtttgaa
    37681 tttgacacac ttatcaagca tttagaaaaa agaggtcttc tacatgatca gcttgcagtt
    37741 ggcggggttt cgatgggggg aatgacaaca tgcgccttgt tggctaaaca tcctcatata
    37801 gtagcgggcg tgtgtttgat gggttcccct gcacctttgg aatatggaaa agaaatcgta
    37861 aggcgcgcat cggaatataa ttatgtcgta ccaaaagact attttgattt gatttcatgg
    37921 gttaagagct atgatttgtc attgcagccg gaaaagttag ctggacgtcc tctgttcttt
    37981 tggcatggac aagctgacga aaaaatccct tatatacaaa cggaacgatt tgtagaaaaa
    38041 aatcggcata agtcttacgg aaaagaaatt gtttttcgtt cctcaaaaac acagcgacat
    38101 atggtacaaa taccactaat ggaagaagct tccacttttt tagcaaaaaa tttgataaaa
    38161 aagtataaat aattgaaacg gaaaatcact catgacgata gaggattttc cgttttttat
    38221 tttgggctat aataaactct gttaggaaaa tttggtataa ttgatagtaa gaatgacaac
    38281 atgttagaaa ggaagagagc atgcccactt tagcagatgt tgcaaaacgt gccaatgttt
    38341 caaaaatgac ggtctctcga gtcatcaatc atccggaaca ggtgactgac gaattgaaag
    38401 agttagtttt ctcagcaatg gctgaactgg attaccttcc aaatattgct gcaaaagcac
    38461 ttgtcacgaa tcggtcgcag atcatcaaat tatttatact ggaagaaatc gacacaactg
    38521 agccctatta catgaatttg ctgatgggaa tcgctaaacg gatcggtaaa gcgcattatt
    38581 ctctccaatt agttacaaat gatgccttcg acgtcggttc ttgtgatgga tacatcatca
    38641 caggtgtcag aaaaaaagac ttcgattgga tcgaccgttt ggaaaaacca gttgtcttgt
    38701 ttggggaaaa ttctgaagga tatgattatg tagacagcga taatgaatat ggtacagcaa
    38761 aagcaacgca atatgcctta gaaagagatt atcaaacgat tatttatatc ggtatcgaag
    38821 tggacgagcc ttttgaaatt tcaagggaaa ctggttattt atctgtgatg aaggaagttg
    38881 atcttcctag taaaatcttg cggtttccaa atcattcgac agaagtagaa agatatatga
    38941 cagaaaactg ggcagatttt acagataaaa cttgttttgt atgtagttca gatcggctag
    39001 cactaggagc agtaagaggg attataaatg ctggaggaca cttgcccgaa gaatttggtg
    39061 tgattggatt tgatggggta tttttagacc aaatcagttc acctaaattg acaacgatca
    39121 agcaagatat tatacgtatg ggtgaagtat gtggagaaat gttgttaaaa aaaatagaag
    39181 aaaaaggggc gagtcaaggt tatcgtcgtt tttttcctga actagttatc agagagacaa
    39241 cgaattaagt cttattacta tatatatatt tatgaaattc atcgctattt tcttaaaata
    39301 atcagcgcat tgctactgaa aacaatgaat ctgtgctatt attgagaaaa gttccatttt
    39361 tgagaaggat ggtttgaatg aaagaaatca gacgaaaaca tttccgtttt cctgcgtacg
    39421 atgatgagtt aggcgtaaaa ttaaccaacg acaagaagcg agtattattt gacggacaag
    39481 aagatctttt gactgaacag aatcagtccc cgtttgaaca gatgaaagaa tttcatttca
    39541 cgaacacgaa caccgagaaa atccgtcgca agcgtcgtgc aacaaatgga ttgaagtcag
    39601 ctccgcgtaa ggaaaatttg gaaaagcata aaggaaaatt accggattat accggccatt
    39661 accaatcaga aatcacatcg acaggacgaa aaaaactttt tggtgattcc tctcgttctg
    39721 tcagacaaag tgaaacaagg acagagtcca agcatactac acgaaacgca tctgttgaca
    39781 cgaaggtgcg ggagcgttct tattttgttc ctaaatatgt acccgcatcg attatcccag
    39841 accctagaga aagcaaaatt tctgaggatc atttaatcga atcgatgaaa aaaacggatg
    39901 actcttatct gttgttcgat acagaaccga cgccgtatca agaaaaaaga aatggtgagc
    39961 cgtctgtacg aacattcaat cgaacaaaaa cgttagagga aacaaagcag tcagaagaaa
    40021 aaaaatcgaa aaaaagccat ggtatcttag aacgctcatt acaaggattg atcgatgatc
    40081 aaggaaactc tttgggagaa aatagctatt ttaaataagt gatatacaaa tttatattcg
    40141 gctacagttg gaaaggaaac gctgcggcgt ttccttattt tatactctat ccgaaagttt
    40201 atatcgtatc ctcaaatttt tttgagttta agcgaatttt ttctcccaat aagtaagaaa
    40261 ataatggcaa aataaaaaaa caaactattt tcgcagatcg tttgcaaaaa tagtttattt
    40321 ttttataaag aagattttac tgaataatat cattttagtt tacataatga tatttctgta
    40381 aattaaataa agtagtgaaa aataagaatc agtgctctaa ctgttcaaga ggatcaagtg
    40441 gttcaaatgt ttctgtagtc tgttttgctt ccaattgggg caaaacagat ccaatttcca
    40501 gatacttcac tttattcaat gtataagata gaatagaaca gctaagtaat gtatacagaa
    40561 aatgtgtgag atccaaataa agcaaggaga caactaaaat accaaaagta atcaagaaaa
    40621 gacttttgcc aatcttaata tgaaaatatt tttcaatggc ttgataaatg atatctgttc
    40681 cgcctgtgct tccgccgaat cgaaagacga gaccgcaacc aatgccagaa agaatacctg
    40741 tcatcaaact attgatcagt agactatcta cagcaaaatg tatatgaagg ctctcccata
    40801 aatgtaaaaa gaaaactagc gcaaatgttc cgacgatcgt ccggagaaaa gcgtgttttc
    40861 caaaaagaaa caatgagata agcaataaag gtaaattaaa aagcagtgaa gtgacagaag
    40921 gagcgaatcc aaaaagatta tgcaaaagca aagcaatacc agtaacccca ccatctgcca
    40981 gcttagatgg aatattgatt cctgtcacag ctaatccgta gattcctgcg cctaaaatga
    41041 ttccgcacaa ttctgtaaat aaatttaatt ttttcatttc tagcctcttt tcttttgaaa
    41101 attttcagca acttctttat tatactgact tatactctta cttcaagagg aaaatgaaaa
    41161 aaaattattt ttggctgttt ctttttattt atcaaataag gagtggaaaa atgcgagcag
    41221 cagacttatt gacaattctt acaaaatcac ccaaaaaaac agagtatcaa ggagtttttt
    41281 tggctcaaga acaaaaactg gtgcctgtga caatggtcca gataaataaa gcaaatcaat
    41341 ttatcttttt atttgaacac ggcaaagcac cacttgctat gaaagacatc tttctttttt
    41401 tgatgaagaa ccgtgagaaa gctgtttatt atcagtcaaa agaagaactc attcctcttt
    41461 atggagtgaa agagatagaa aataaagtgg tgatataagt aaaaataaga aaacaagacg
    41521 tttatgatgt caaaacaacc attttttaca tttttggcaa aaaagtgtca tgatacagta
    41581 taatattcgt tgaacaggcc aataaaggtg ttctccccca cgagatttct actttattgg
    41641 ctgtttcttt ttattaaaat aaaagttgtg tgtgtaaaaa agatatagct gattccaagt
    41701 gacaatttcg tatagatttt tatggtattc gtagtgaaaa aacgagaacc aaaaagttga
    41761 tccgactttg ttgcttggat tctttttttg tcagcatatt tagaggtatt tttttctttt
    41821 ttttgttcgt gctgtaaggt taaggcaaga caagttagtt gggaaaaagg aaagaaatgt
    41881 ttatacttaa agaataacag aaaggaaggt cgcatgaata attttataca aacattaagt
    41941 gaaagaaaag gcgagttgct ggaagctgca tttcaacatc tgtccatttc tctcatttct
    42001 ttgctgattg cagcactgat tgctattcct ttagcgattt gggcagccaa tcataaaaaa
    42061 atggcagaag tcttattgca gatcacgggt gtgctccaaa cgattccttc acttgcctta
    42121 ttaggtttac ttatcccatt tgtagggatc ggaactgttc cagcattgat tgcacttgtt
    42181 atttacgctt tgcttccgat tttccaaaac acgtatattg gtctagctga aattgacccg
    42241 tctattgaag aagcagcggt agcatttggt atgtccagaa tgagacgctt gctgaaagta
    42301 gagcttccca tcgcattacc tgtcattatc tcaggtatcc gaactgcctt agttttaatc
    42361 atcggtacgg ctactttggc agcattgatc ggagcaggtg gattagggac atttattttg
    42421 ttaggtattg accgaaatac acctgttttg acattgattg gtgcgatcag ttctgccttg
    42481 cttgcaatta tattcagcgg actgattcgc tggttgcagc ataagaaacc tcgatattcc
    42541 atcattactt tagcagcagt ggtgtgttta tttggcggcg tttctttata tcagtctgcc
    42601 ttgtttaaaa atgaaaccat cacgattgct ggaaaattag gagcggaacc tgaaatcctg
    42661 atccaaatgt ataaagaatt gattgaagac aacacaaaaa cagaagttga attaaaaccg
    42721 aattttggca agacgagttt tttgttcaat gcactggatc aaggtcaaat cgatatctat
    42781 cctgagttca caggtactgt cctagaaagc ttggtgaagg tacctgaagc aatgaaaagt
    42841 gctgatttga caaaagaaaa aacatatgac gaagcagaag aattattgaa taagcaattc
    42901 gatatgacat tattaaaacc aatggcttac caaaatacgt atgccttggc agtcaagaga
    42961 tcttttgcga acgaacatca acttaaaaag atttccgatt tggcagctat ctctgatcag
    43021 atcaaagcgg gattcacact ggaatttatt gatcgttcag atggatataa aggaatccaa
    43081 gaagcatatg gcttgaattt ttcaagtgta caaagcatgg agccaagttt gcgttaccaa
    43141 gcaatcaata atggagatgt caatattgtg gatgcctatt ccacagatag ccagctcaag
    43201 gagtacgatc tagtgactct agaagatgat cgagggttat tcccagctta tcaaggcgca
    43261 ccgctgatga aaaaagattt tgctaaagaa catcctgaaa tcgttaaagc actgaataaa
    43321 ttaagcggaa aaataacaga ggatcaaatg attgatatga attaccaagt caatgtcgaa
    43381 aagaaaaagc cgacagaagt agcacgaatc ttcctgaaaa atgaaggatt agtaaaggag
    43441 gaagatcagt gactaaatta atcgagttca aacatgtgca aaaaaagtat gatggaaagt
    43501 atgtcatcga tgacctaaat ctggcaatca aaaaaggaga aatctttgtt ttggttggtc
    43561 cttccggtag tggaaaaaca actactttga aaatgatcaa tggattatct aaaccatcag
    43621 caggagacat ctacttcaaa ggaaaatcgt taaatgaata taatctgcag aaaatgcggt
    43681 ggaatatggg atatgtgctg cagcaaatcg ctttgttccc gaccatgaca gtcaagcaaa
    43741 atattgaagt catccctgaa atgttaggtt gggaaaagca gaaaagagca gaccgtgtag
    43801 atgaacttct tcaaaaagtt ggcttatctc ctgatattta tcgagatcgt atgcctagag
    43861 agttgtctgg aggagaacag caacggatcg ggatcatccg agctattgct gcaagcccag
    43921 atgtgatttt gatggacgag ccatttagcg cattagatcc gatttcaaga aactcattac
    43981 aagaattggt tttgtcctta cacgaagaat tgggcacgac catcgttttc gttacgcaca
    44041 atatggaaga agcaatcaag ctaggtgatc gtattgcatt tatgaaggat ggagaaatta
    44101 tccaatgtga cacaccagag cagttattga tgaatcctaa aaacgactac gttcggcatt
    44161 tctttgatga accgaaacag acgaaagagt ggcgagtaga agatttagtc gtcaatggat
    44221 atttcttaaa tgaaatacca gaaaatgctc gtcaacaagt ttgctttgat acgcctatga
    44281 aagaagtata ttcgcttctt tcggtgtatc caagcattgt gatagtagaa aagcaaagaa
    44341 gtattgggtc gctgacaagt aaagaaatct ttgctttttt aagtcgagag gaggaaggaa
    44401 atgaaaacat atgatgcaat catcatcggt tcaggtgtga gcggcttaag tgctgcttat
    44461 ggattgaaag aagcaggtaa aacagtacta gtggtcgaag aagatctatg gggcgggact
    44521 tgtcctaacc gtggatgtga tcctaaaaaa gtcttgttaa gtgcagtaga ggcacgtaat
    44581 cgggtaaaac aattgagtgg aaaaggtttt aacgagatcc caactgccaa ctgggaagaa
    44641 cttcaaaaat tcaaacgaac atttacggat ccagtcccag aaagccgaaa aaaacaatta
    44701 gcagaagctg aaatcgatca tctttcagga accgctcgtt ttttggatga ttcatctatt
    44761 gaagtcaacg aagaagtctt ccatgcggat tacttagttt tggcaaccgg gcaaagacct
    44821 acgatcttac cagtggaggg aaaggaatac ttgaaaacta gtgcagactt tttatcattg
    44881 cctgtactgc ctaaagaaat catttttatc ggtggtggtt atattgcttt tgagctagct
    44941 acgattgcca atgctgcagg gagcaaagta accattgtcc atcataatca acggccatta
    45001 aaagaatttg aggcgtcttt ggtagaggaa gccgtccatc aaatggaagc atctggcatt
    45061 cagtttgctt ttggtgttga aacccaaaag atcatttcag aaggtactcg ctatcgttta
    45121 gtcggaaaag agacggaatt agtagcagat atgatttttt gtgcgactgg gagacagccg
    45181 aatacagaat ctttagcact tgagcaagcg aatatcgtct ttgataaaca cggaattgca
    45241 gtcaatgatt atctgcaaac gagcaatcca aaaatttttg cgtgtggaga tattgtttca
    45301 aggaaaaccc caaaattgac accagtcgcc acttttgaag gaaattatgt agctaagcgg
    45361 ataacggatg caacaagcga acctatcaaa tatccgatta tacctacgat cgtctacgcc
    45421 agtcctaaac ttgctgaagt aggagttaca aaaagtcacg cctcttcgtc agatcaagtg
    45481 gtagagatgg atcttaccag ttggttcacc tatcatcgtg tcaatgaacc agttgctaag
    45541 gctgaattga cctttgacca gcaaaattat ttgattggtg ctgcagtgat tagcgagcaa
    45601 gcagacgaat tgatcgatga tttgacactc gtcatcaatc aaaaattgac taaaaaagag
    45661 ttggatagct atatcatggg gtatccgaca ttggcaagtg atctctccta tttattgaaa
    45721 taatcagcat gataactaaa gcgcttgttg ctttttcctt cttgtgctac actttaaaaa
    45781 atgaatacct gtttctgggg atgtcgtaga cgacatgaga gtaagttgtg aacttagacc
    45841 cattgaacct gtcagttaat actggtgaag ggagaaacat agcagacgct tgaagcgggt
    45901 tactatgtcg tgcccatttc aagcgttttt tgttattcat attagaagaa aggaggtaac
    45961 aaaatgaaga aaaatcttta tccagcagtc agtatggcat tcattttaat tatttggcag
    46021 attgttgtca tagtttggaa aatacctgcc tttattttac ccggacctgc tgctgtattt
    46081 tctgcattag cggcagatgc acaagtatta ctttcccatt ctgtcacgac attaaatgaa
    46141 gcagtgcttg gtttggtaat agctagcgtt ctttctttta ttactgcctt ggcaatggat
    46201 tattggaagt ttttagaaat gtctttttat ccgttactag tgatttcaca aatgttgcca
    46261 atcatggtgt tagggccatt acttacccta tggtttggtt tcggtatgct tccaaaagtg
    46321 atccttgttg tcttgataag ctattttccg atcgtagttt ctttttcgga tacattgagg
    46381 aaagtatcaa aagagcaaat catctttttg aaaacgatgg gggcagatac agggaagatt
    46441 tatcgaatat ataaaatacc agcgggttta tccggcttct tttccggttt aaaagtggct
    46501 gctacttatt gtgtcagcgg agcagtcgtt ggagaatggc taagcgctca agcaggctta
    46561 gggtattaca tgatacgcgt aaaaaacagc taccagatcg ataaagtttt tgctgcgatc
    46621 atttgtgtca ttctattgag tttattgctg aatggcgctt gttcattatt gaaaaaaggg
    46681 tattatcgta tgctttatat tttttaagag aaggggatca aatgaaaaaa ttatggcttt
    46741 tattaccgct actgttgctt ttatccgctt gtggcactgc aaaagaaaca agtagcaaac
    46801 aagaaataaa ggatttgaaa aaagtgacac ttgttttgga ttatgtaccg aatactaatc
    46861 atactggcat ctacctagca aaagaaaaag gctattataa agaggctggt ttaaatgtcc
    46921 aaatcatcga accaggagat aattcgacaa gtattggtct agtaggtgcg gacaaagcac
    46981 aatttggtgt aagttatcaa gaagatgtca cttatgctca cgcagatgga cagaatatac
    47041 cagtcaaagc aattgctaca gtgatcaaac ataatacttc tggattcgcc accctatcag
    47101 acagtaacat ccattcacca aaagattttg aaggaaaaac atatgcaggt tggcaaagtc
    47161 caagtgaaga agctgtctta aaagctgtga tggaaaaaga cggcggggac ttttcaaaac
    47221 tgacaatggt aggaagcaat ggagaagggc cagaaagctt aggaaaatcc tcagatatcc
    47281 aatggtactt tgaaggttgg gatatgatca aagcaaaaga agcgggtatt gaactaaact
    47341 atatcccatt gaaagaatta gatgaacggt tagattacta tactccggta attattacga
    47401 atgatcaact gatcaaaagt gatccagaat tggtacagtc ttttatggat gccacgaaaa
    47461 aaggctatca ggaagctatt aaggatccaa atgacagtgc taaactgttg caaaaatatg
    47521 caaaagaaaa tgatcggacg tttttagaag aatcacaagc attcttgtca aaaaattata
    47581 cggatgatcc tgagaattgg ggattgatgg aagaaaaagt ttggcgcaat tatacagctt
    47641 ttatgcagga aaacggattg atcaagcaag atgtaccttc gcaagaacta ttcactaatc
    47701 agtttataaa ataggagtga tagatatgtc aatccaaatc agccacttga atgtagtcat
    47761 tgaaaacctg aaaatattag aagacatcca tttcactatc cctaaagata cctttacatc
    47821 gattgtcgcg ccaagcggag ccggtaagtc aacattgctg aaaactttaa caggagtcca
    47881 gcctatgact tctggaacga tcaaagtaga cgatcaaaaa gtaactggtc tccatacagc
    47941 atttagttat atgccgcaag aagatatgtt acttccttgg ctgagcgtct atcaaaacgt
    48001 tacgttatat caaaaaatca atcatctaac aatcaatgaa gaacaagtaa gggattatct
    48061 ggatatcttt gggttaaacg gctatgaaca ttttttgccg gaacaattat ctggcgggat
    48121 gaagcagcga acggccttgt tacgaacaat catgaatcct tcttcttact tgcttcttga
    48181 tgaaccattt ggagcgttag atgctatgac cagagggcag atgcaagact ggatgttgaa
    48241 gctgcctaaa aaagcaaagc ggactacttt acttgtgacg catgatatcg aagaagcaat
    48301 ttatttatca gacaggattc ttgtgctttc cgcccgtccg gcacatgtga ttgctgagat
    48361 tcaagtgcct gaaaaacgac ggtcacgtga atggctactg cagcaatcag agttgaagca
    48421 aacgatttat cagttattgg caggtgaatt agatgttaag tgatgctcat tgtcatttag
    48481 atgggagtca gagtttagca ctccttcagc aggaacacac gattttgacg atcatcaatt
    48541 gtgattcgcc agaagaatgg aaggaaaaca gacagcttgc agcaagtaag actcaagcct
    48601 taagttacgg tatccatcct tggaaagcag atagctatac gtttgaacaa gttgagcctt
    48661 ttttgaaaaa agcaaggatc atcggtgaaa tagggttaga taatatttgg acgaatgtac
    48721 cgatgactac acaaaaaaaa gtatttgagc gacagcttgc ttttgcagcc ataaatgaga
    48781 aacctgttgt actgcacaca aaaggatgcg aaaaagagat cttaaattat atccaagctt
    48841 accctaatca ctatttgatc cattggtatt cttctttgga ctatcaacaa gactatattg
    48901 atttaggctg ttatttcagc attggtcttg atttgaaaaa gaatccagca gtctggcaac
    48961 tagctaaagc agtcccaatc gaccacttac ttattgaaac ggatggacta catgcagcag
    49021 agtgggcttt aggggggatt ttttcagaaa aagattatgt ctcactgctt gaagaacagt
    49081 taaagacaat cgctgagatc aaagagctat cagttgaaga agttaaaaaa aatacttatc
    49141 aaaacttgga acgtttcatt agattaggta aataaaaaag agatgtgaca caactagtgt
    49201 cgcacctctt tttggctttc tttgtataaa agtaaactgt ttgattaata aatgaatctt
    49261 agtaccaacc gtttgcttcc cagaatgctt tcgccgcttc ccaagaacca taacgtgaag
    49321 taacatattg ttctgctact ctttcttggt ttgcagcaga gtagtcacca tttaagtaag
    49381 aagaatctaa ttggtaacgt ccgatgtaac gtccgttcgt tgctgtgtaa gaaccgcttg
    49441 attctttttg tgcgatccat tcttttgcag agcttgtgct tgctggagta gcagcagttt
    49501 ctgttacagc aggagcaggt tgtgtttcag caacaggcgc tgcttgtttt tccgtttgaa
    49561 ccgtttcttg tacaacaggt tgttcagctt ggactggtgc ttcttgaacg gctgcttgag
    49621 gttcagcagc tgcttgtgca gagccttctg ttgggattgt taattgttgt cctgagta
//
LOCUS       DACECR010000010        47271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.010, whole genome shotgun sequence.
ACCESSION   DACECR010000010 DACECR010000000
VERSION     DACECR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.010"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(91..777)
                     /gene="gpmA"
                     /locus_tag="IWO17_000510"
     CDS             complement(91..777)
                     /gene="gpmA"
                     /locus_tag="IWO17_000510"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0289409.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(949..1530)
                     /locus_tag="IWO17_000511"
     CDS             complement(949..1530)
                     /locus_tag="IWO17_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0289410.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1663..2346)
                     /gene="rpiA"
                     /locus_tag="IWO17_000512"
     CDS             complement(1663..2346)
                     /gene="rpiA"
                     /locus_tag="IWO17_000512"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ0289411.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2665..3078
                     /gene="rpsL"
                     /locus_tag="IWO17_000513"
     CDS             2665..3078
                     /gene="rpsL"
                     /locus_tag="IWO17_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ0289412.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3162..3632
                     /gene="rpsG"
                     /locus_tag="IWO17_000514"
     CDS             3162..3632
                     /gene="rpsG"
                     /locus_tag="IWO17_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ0289413.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3726..5813
                     /gene="fusA"
                     /locus_tag="IWO17_000515"
     CDS             3726..5813
                     /gene="fusA"
                     /locus_tag="IWO17_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0289414.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5985..7172
                     /gene="tuf"
                     /locus_tag="IWO17_000516"
     CDS             5985..7172
                     /gene="tuf"
                     /locus_tag="IWO17_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0289415.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7579..7887
                     /gene="rpsJ"
                     /locus_tag="IWO17_000517"
     CDS             7579..7887
                     /gene="rpsJ"
                     /locus_tag="IWO17_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ0289416.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7918..8547
                     /gene="rplC"
                     /locus_tag="IWO17_000518"
     CDS             7918..8547
                     /gene="rplC"
                     /locus_tag="IWO17_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ0289417.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8575..9198
                     /gene="rplD"
                     /locus_tag="IWO17_000519"
     CDS             8575..9198
                     /gene="rplD"
                     /locus_tag="IWO17_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ0289418.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9198..9488
                     /gene="rplW"
                     /locus_tag="IWO17_000520"
     CDS             9198..9488
                     /gene="rplW"
                     /locus_tag="IWO17_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ0289419.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9527..10360
                     /gene="rplB"
                     /locus_tag="IWO17_000521"
     CDS             9527..10360
                     /gene="rplB"
                     /locus_tag="IWO17_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ0289420.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNRAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10403..10681
                     /gene="rpsS"
                     /locus_tag="IWO17_000522"
     CDS             10403..10681
                     /gene="rpsS"
                     /locus_tag="IWO17_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ0289421.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10704..11051
                     /gene="rplV"
                     /locus_tag="IWO17_000523"
     CDS             10704..11051
                     /gene="rplV"
                     /locus_tag="IWO17_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ0289422.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11065..11721
                     /gene="rpsC"
                     /locus_tag="IWO17_000524"
     CDS             11065..11721
                     /gene="rpsC"
                     /locus_tag="IWO17_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ0289423.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11724..12158
                     /gene="rplP"
                     /locus_tag="IWO17_000525"
     CDS             11724..12158
                     /gene="rplP"
                     /locus_tag="IWO17_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ0289424.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12148..12336
                     /gene="rpmC"
                     /locus_tag="IWO17_000526"
     CDS             12148..12336
                     /gene="rpmC"
                     /locus_tag="IWO17_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ0289425.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12361..12627
                     /gene="rpsQ"
                     /locus_tag="IWO17_000527"
     CDS             12361..12627
                     /gene="rpsQ"
                     /locus_tag="IWO17_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ0289426.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12685..13053
                     /gene="rplN"
                     /locus_tag="IWO17_000528"
     CDS             12685..13053
                     /gene="rplN"
                     /locus_tag="IWO17_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ0289427.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13090..13398
                     /gene="rplX"
                     /locus_tag="IWO17_000529"
     CDS             13090..13398
                     /gene="rplX"
                     /locus_tag="IWO17_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ0289428.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13426..13965
                     /gene="rplE"
                     /locus_tag="IWO17_000530"
     CDS             13426..13965
                     /gene="rplE"
                     /locus_tag="IWO17_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ0289429.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13984..14169
                     /locus_tag="IWO17_000531"
     CDS             13984..14169
                     /locus_tag="IWO17_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ0289430.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14205..14603
                     /gene="rpsH"
                     /locus_tag="IWO17_000532"
     CDS             14205..14603
                     /gene="rpsH"
                     /locus_tag="IWO17_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ0289431.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14635..15171
                     /gene="rplF"
                     /locus_tag="IWO17_000533"
     CDS             14635..15171
                     /gene="rplF"
                     /locus_tag="IWO17_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ0289432.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15332..15688
                     /gene="rplR"
                     /locus_tag="IWO17_000534"
     CDS             15332..15688
                     /gene="rplR"
                     /locus_tag="IWO17_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ0289433.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15709..16209
                     /gene="rpsE"
                     /locus_tag="IWO17_000535"
     CDS             15709..16209
                     /gene="rpsE"
                     /locus_tag="IWO17_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ0289434.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16224..16403
                     /gene="rpmD"
                     /locus_tag="IWO17_000536"
     CDS             16224..16403
                     /gene="rpmD"
                     /locus_tag="IWO17_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ0289435.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16447..16887
                     /gene="rplO"
                     /locus_tag="IWO17_000537"
     CDS             16447..16887
                     /gene="rplO"
                     /locus_tag="IWO17_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ0289436.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16887..18182
                     /gene="secY"
                     /locus_tag="IWO17_000538"
     CDS             16887..18182
                     /gene="secY"
                     /locus_tag="IWO17_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ0289437.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18244..18891
                     /locus_tag="IWO17_000539"
     CDS             18244..18891
                     /locus_tag="IWO17_000539"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ0289438.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19082..19300
                     /gene="infA"
                     /locus_tag="IWO17_000540"
     CDS             19082..19300
                     /gene="infA"
                     /locus_tag="IWO17_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ0289439.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19333..19449
                     /gene="rpmJ"
                     /locus_tag="IWO17_000541"
     CDS             19333..19449
                     /gene="rpmJ"
                     /locus_tag="IWO17_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ0289440.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19467..19832
                     /gene="rpsM"
                     /locus_tag="IWO17_000542"
     CDS             19467..19832
                     /gene="rpsM"
                     /locus_tag="IWO17_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ0289441.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19860..20249
                     /gene="rpsK"
                     /locus_tag="IWO17_000543"
     CDS             19860..20249
                     /gene="rpsK"
                     /locus_tag="IWO17_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ0289442.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20329..21267
                     /locus_tag="IWO17_000544"
     CDS             20329..21267
                     /locus_tag="IWO17_000544"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ0289443.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21295..21675
                     /gene="rplQ"
                     /locus_tag="IWO17_000545"
     CDS             21295..21675
                     /gene="rplQ"
                     /locus_tag="IWO17_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ0289444.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21947..22786
                     /locus_tag="IWO17_000546"
     CDS             21947..22786
                     /locus_tag="IWO17_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0289445.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22762..23631
                     /locus_tag="IWO17_000547"
     CDS             22762..23631
                     /locus_tag="IWO17_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0289446.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23621..24418
                     /locus_tag="IWO17_000548"
     CDS             23621..24418
                     /locus_tag="IWO17_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0289447.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24621..25367
                     /gene="truA"
                     /locus_tag="IWO17_000549"
     CDS             24621..25367
                     /gene="truA"
                     /locus_tag="IWO17_000549"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0289448.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25822..28491)
                     /locus_tag="IWO17_000550"
     CDS             complement(25822..28491)
                     /locus_tag="IWO17_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0289449.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28729..28917)
                     /locus_tag="IWO17_000551"
     CDS             complement(28729..28917)
                     /locus_tag="IWO17_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ0289450.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29187..29549
                     /locus_tag="IWO17_000552"
     CDS             29187..29549
                     /locus_tag="IWO17_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0289451.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29601..31283
                     /locus_tag="IWO17_000553"
     CDS             29601..31283
                     /locus_tag="IWO17_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ0289452.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31400..33436
                     /gene="recG"
                     /locus_tag="IWO17_000554"
     CDS             31400..33436
                     /gene="recG"
                     /locus_tag="IWO17_000554"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ0289453.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33485..34486
                     /gene="plsX"
                     /locus_tag="IWO17_000555"
     CDS             33485..34486
                     /gene="plsX"
                     /locus_tag="IWO17_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ0289454.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34608..34850
                     /gene="acpP"
                     /locus_tag="IWO17_000556"
     CDS             34608..34850
                     /gene="acpP"
                     /locus_tag="IWO17_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0289455.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            35118..36122
                     /locus_tag="IWO17_000557"
     CDS             35118..36122
                     /locus_tag="IWO17_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0289456.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            36123..37067
                     /locus_tag="IWO17_000558"
     CDS             36123..37067
                     /locus_tag="IWO17_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0289457.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            37067..38029
                     /locus_tag="IWO17_000559"
     CDS             37067..38029
                     /locus_tag="IWO17_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0289458.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            38056..38970
                     /locus_tag="IWO17_000560"
     CDS             38056..38970
                     /locus_tag="IWO17_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0289459.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38996..40777
                     /locus_tag="IWO17_000561"
     CDS             38996..40777
                     /locus_tag="IWO17_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0289460.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            41125..41811
                     /locus_tag="IWO17_000562"
     CDS             41125..41811
                     /locus_tag="IWO17_000562"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0289461.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41831..45412
                     /gene="smc"
                     /locus_tag="IWO17_000563"
     CDS             41831..45412
                     /gene="smc"
                     /locus_tag="IWO17_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ0289462.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45421..46230
                     /locus_tag="IWO17_000564"
     CDS             45421..46230
                     /locus_tag="IWO17_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0289463.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46241..47239
                     /gene="ftsY"
                     /locus_tag="IWO17_000565"
     CDS             46241..47239
                     /gene="ftsY"
                     /locus_tag="IWO17_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ0289464.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
BASE COUNT        15661 a         8641 c        10233 g        12736 t
ORIGIN      
        1 ttgtgaccga gaagttggca ggagaaaacc gtttatacga aaaaagggac tgtgacaaat
       61 cgtgtcacag tccctttttt atagtgtttt ttataggtag tattttttag caactgttaa
      121 gtcatcattt aattcataaa caagtggttg acctgttgga atttcaagat ccatgatgtc
      181 ttcgtcagag ataccttcga tatgtttagc tagggcgcgt aatgagttac catgtgcagc
      241 tactaaaaca gttttgttgt ctagtaatgc tggagcaatt tcatcttgcc agaatggcaa
      301 agcgcgttct aatgtcactt tcaagttttc tccaccagga atatcgcgtt tgtctaacat
      361 tgcgtaacga cggtcatttg ctgcagaacc ttcgtcagtt tcttccatta atggaggaag
      421 agtatcgtaa gaacgacgcc agatgtgcac ttgttcgtcg ccgtattttt cagctgtttc
      481 ttttttgttc aaaccttgta atttaccata gtgacgttcg tttaagcgcc atgatttgat
      541 ttgtggtacc caaagttgat ctgaatattc taagatcaag ttacatgttt tgattgcacg
      601 agttaaaaca gaagtgtaag caacatcaaa ttcgattcca gcttctttga ttttgcgtcc
      661 gccttctttt gcttcttcga ttccttctgg tgctagattt acatcagccc aaccagtaaa
      721 ttggttcaat gcattccatt cactaagacc gtgacgagaa aaaactaatt ttggcatgtt
      781 tagctctcct tttaagatag gtttatattt ctgaaatttt cagttctgtt ttattgtaca
      841 attttttgga agagatttcc atcgaaaaat tagataagat tctatgagaa aacaagtaga
      901 gaatataaaa aaacgaacag tgcccacgca actgttcgtt tttaggtatt agatgcataa
      961 ttgctcgatt tctttttcag gtaaattgat atatttataa catgttccaa ctgaaacacc
     1021 gcattttgaa gaaataaatt ggatcgtttc ttttttttca taatagagac gtcggatttt
     1081 tttgatcgtt tttgcatcga tttttggtct tccgccgatt tttccttttt tacgagcttc
     1141 atctaatcca actaatgtac gttctttgat caatgcgcac tccatgtttg ctaggttttc
     1201 cattaattgg aaatagattt ttcccatttc gcgcgtatcg attccttcat caagactaat
     1261 caagtgcagc ccgcgatctt tgaataattg cgtgagttct gttaattgcc gcgtagattt
     1321 gcctaatcga tgaagttggc aaatgactag tgcatcgcct tgttgcatgt cagcgatcac
     1381 tgactccaaa tgaacagcag tagaatcatt ttgttccact tcatatgtct catggaaaat
     1441 ttcttgacaa ccaaagtcag ataattgatc aacctgagtt gttaaatcaa cgtcatttat
     1501 ggttgtgcgt gcataaccaa taattttcat ggtgattcct ttctagcagt gattttctaa
     1561 ctggatataa cagtaaaaat gtagcataaa gtacgtcaaa aagatagttt tttcgcaaat
     1621 tttccgctgt ctttttttat tggatgatca agcagttttt ctttatttac gacggattgc
     1681 gtcaagaatc tcaacgcctt tttctgagcc gacaactact ttagaaacca tgtttaggaa
     1741 aagcccatgt tctactaccc caacaagttc atctaaatag gtagctaagc tttctggctg
     1801 ttcgatgact tctagatgca gatcaatgat gtaatgacca ccatctgtgg ttaagacttc
     1861 tccgttttcg tctgtacgca agacgggttg gaatcttttt tcttcaaaaa tatgaagtag
     1921 ctgttgggag ccataaggga tcacttcaac tgggagtggg aacttcccaa gtttatcgac
     1981 taatttagaa gaatcaacga tccaaatcgt ttcttttgag tacgtagcga ctattttctc
     2041 gaaaagcaaa gcagcgccgc cgcctttgat cccttgaaaa tcagcactga tttcatcagc
     2101 accgtctatt gtcaaatcga ctacggggac ttcatcaata ctctttaacg gtattcctaa
     2161 tttttccgct tgttcttttg tcgcatttga agtagtaact cctgtgatac gtaaaccttc
     2221 ttcacgcata cggcgaccaa tctcttctac catataatag gcggttgaac cggtcccgag
     2281 acctacgatc atcccgtcct ttacgaattt cgcggcttca atgccagcta attgttttaa
     2341 attcatcttt gcgtccacct ttttttagtt tgagcatcgt gctttcctca ctattttagc
     2401 gtgatttacg gtaaaaagat accattcttt tttattccaa taaagctttt tcaaaaaaaa
     2461 ttgcatctct tgttgacatg ttttttcatt cgtgatattc taacaaaggt gctttatcta
     2521 caggtttctg tgaagaaacg tgctgactgt ttatcaaagc atatttgata aggatgcaga
     2581 cattgcattt attttttatc acaaaaaaag aaccacctgg atgtgtggaa ctagacgcaa
     2641 atcgtgggaa ggaggaaaca aggaatgcct acaattaacc aattagtacg taaacctcgt
     2701 aaatcaaagg tggaaaaatc taattcacct gctttgaaca aaggatataa cagctttaag
     2761 aaaactcaaa ctaacgtaaa ctcacctcaa aaacgtgggg tagcaactcg tgtgggtaca
     2821 atgacaccta aaaaaccgaa ctcagcttta cgtaaatatg cacgtgttcg tttgtcaaac
     2881 ttaattgaag ttactgctta tatcccaggg attggtcata acttacaaga acacagtgtt
     2941 gtattattac gcggtggtcg tgtaaaagac ttaccagggg tacgttacca tatcgttcgc
     3001 ggtgctttag atactgctgg tgttaacgac cgtaaacaaa gccgttctaa atacggaact
     3061 aaaaaaccta aagcttaatt ttaattatta aacaaatgaa acttgcttct actatataga
     3121 agtaattact atgaacaata tttcggaagg aggagttacg gatgccacgt aaaggtcctg
     3181 ttgcaaaacg tgatgtttta ccagatccta tttataactc aaaattagta actcgcttga
     3241 tcaaccgtgt aatggttgac ggaaaacgcg ggattgcagc taacattatc tataatgcct
     3301 ttgacttgat caaagaatct acaggtaacg atccattaga agtgtttgaa caagcaatga
     3361 aaaacgttca acctgttctt gaagttaaag cacgtcgtgt tgggggttca aactaccaag
     3421 taccagttga agttcgccct gaacgccgcg ttactctagg tcttcgttgg gtagtaaact
     3481 acgctcgctt gcgcggtgaa cacacaatgg aagaacgctt agcaaaagaa atcatggatg
     3541 ctgcaaacaa cacaggtgct tctgttaaaa aacgtgaaga cactcataaa atggctgatg
     3601 ccaaccgtgc gtttgctcac tatcgctggt aagattctct ctgtttggtc ggcttttata
     3661 agtcgacctc agatagtgtc cttattttta gcaactagat agtaaagaga ggagaaacaa
     3721 ggagaatgac cagagaattt tctttacaaa acactcgtaa tatcggtatc atggcccaca
     3781 tcgatgctgg taaaacgact gctactgaac gtatcttgta ctatactggt aaaatccata
     3841 aaattggtga aactcatgaa ggagcttcac aaatggactg gatggaacaa gaacaagaac
     3901 gtggtattac gatcacttct gctgctacta cagcacaatg gaagggtcac cgaatcaaca
     3961 tcatcgacac accaggacac gtagacttca cagttgaagt tgaacgttca ttgcgtgtac
     4021 ttgatggtgc tgtaactgtt cttgatgcac aatcaggtgt tgaacctcaa actgaaacag
     4081 tttggcgtca agcaacaact tatggtgtac ctcgtatcgt atttgcaaac aaaatggaca
     4141 agatcggtgc tgacttctta tattcagtat caactttaca tgaccgtttg caagcaaacg
     4201 ctcacccaat ccaattacca attggtgcgg aagacgactt cacaggaatt atcgaccttg
     4261 taacaatgaa ggctgaaatg tatacaaacg atcttggaac agagatcgaa gaaacagaaa
     4321 ttcctgaaga ataccgcgaa ttagctgaag aatggcgcga aaaattggtt gaagctgtag
     4381 ctgaaactga tgaagaactt actttgaaat acttagaagg tgaagaaatc actgaagctg
     4441 agttaaaaga aggtatccgc cgcgcaacag ttaacgtaga attctaccca gtactatgtg
     4501 gttcagcatt taagaacaaa ggggttcaat tactattgga cgctgttctt gattacttgc
     4561 catcaccgtt agatatccca gctatcaaag ggattgaccc taaaactgat gaagaagtag
     4621 aacgtcctgc agacgacagc gctcctttct cagctttagc atttaaagtt atgactgacc
     4681 cattcgtagg tcgtttaact ttcttccgtg tgtactctgg tgttttacaa tcaggttcat
     4741 acgtacaaaa cgcaacaaaa ggcaaacgtg aacgtgttgg tcgtatccta cagatgcatg
     4801 ccaactctcg ttcagaaatc tctgaagttt acgctggaga tatcgctgct gctgttggtt
     4861 tgaaagacac aactactggg gacacactat gtgatgaaaa gagtctagtt attcttgaat
     4921 ccatggaatt cccagaacca gttatccaag tcgctattga acctaaatca aaagctgacc
     4981 aagataaaat gggtgtggct ttacaaaaac tatctgaaga agatcctact ttccgtgctg
     5041 aaacaaacgt ggaaacaggc gaaacaatca tcgctggtat gggtgaactt cacttagata
     5101 tcatcgttga ccgtatgcgt cgtgaattca aagttgaagc taacgtaggt gcgcctcaag
     5161 tatcttaccg tgaaactttc cgtgctggca ctcaagccga aggtaagttt gtacgtcagt
     5221 ctggtggtaa aggtcaatac ggtcacgtat ggattgaatt tacaccaaat gaagaaggtg
     5281 ctggtttcga atttgaaaat gcgatcgtcg gtggggttgt tcctcgtgaa tacattccag
     5341 ctgttgaaac tggtttgaaa gatgctatgg aaaatggtgt cttagctggc tatccattag
     5401 tagatatcaa agctaaactt tacgatggtt cataccatga tgtcgattca aatgaaactg
     5461 ccttccgtgt tgctgcttct atggcattac gtgcagcagc taagaaagct aatccagtaa
     5521 tccttgaacc aatcatggct gtagaagttg ttatccctga agattattta ggtgatgtta
     5581 tgggtcacgt aactgctcgt cgtggacgtg ttgaaggtat ggaagctcgc gctggcggcc
     5641 aacaagttgt tcgtgcgatg gttccacttg ctgaaatgtt tggttatgct acaactcttc
     5701 gttctgctac tcaaggtcgt ggtacattta caatgacatt tgaccactac gaagatgtac
     5761 caaaatcagt acaagaagaa atcattaaga aaaacggcgg aaaagctgaa taatttcttg
     5821 aaaccggata gtcctgcaaa gagattatcc aaagagacta tatttttaat atagtttcgt
     5881 gtaaaataat tatcgatgat atgggcatgt ggtgtacctt gtgtacatca tatacctatc
     5941 agacaaataa aaaatatatt gtatttttag gaggacattt taaaatggca aaagaaaaat
     6001 ttgaccgttc taaaccacac gttaacattg gtactatcgg acacgttgac catggtaaaa
     6061 ctacattaac agctgcaatc acaactgtac tatctaagaa aaacggcggc caagctatgg
     6121 cttacgatca aatcgatggt gctccagaag aacgcgaacg cggaatcaca atctcaacag
     6181 ctcacgttga gtatgaaaca gatacacgcc actacgctca cgttgactgc ccaggacacg
     6241 cggattacgt taaaaacatg atcactggtg ctgctcaaat ggacggagct atcttggtag
     6301 tttctgctgc tgacggccca atgcctcaaa ctcgtgaaca catcctattg tctcgtcaag
     6361 ttggtgttcc ttacatcgtt gtattcttga acaaagtaga catggttgat gacgaagaat
     6421 tactagaatt agttgaaatg gaagttcgtg acctattaac agaatacgaa ttccctggtg
     6481 acgatgttcc tgtagttgct ggatcagctt tgaaagctct agaaggcgac gcttcatacg
     6541 aagaaaaaat tcttgaatta atggctgcag ttgacgaata catcccaact ccagaacgtg
     6601 acaacgacaa accattcatg atgccagttg aagacgtgtt ctcaattact ggacgtggta
     6661 ctgttgctac aggtcgtgtt gaacgtggac aagttcgcgt tggtgacgaa gttgaagttg
     6721 ttggtattgc tgaagaaact tcaaaaacaa cagttactgg tgttgaaatg ttccgtaaat
     6781 tgttagacta cgctgaagct ggagacaaca ttggtgcttt actacgtggt gttgcacgtg
     6841 aagacatcca acgtggacaa gttttagcta aaccaggtac aatcacacct catacaaaat
     6901 tctctgcaga agtatacgtg ttgacaaaag aagaaggtgg acgtcatact ccattcttca
     6961 ctaactaccg tccacaattc tacttccgta caactgacgt aacaggtgtt gttgaattac
     7021 cagaaggaac tgaaatggtt atgcctggcg acaacgtaac aatggaagtt gaattaatcc
     7081 acccaatcgc tatcgaaaac ggtactaaat tctcaatccg tgaaggcgga cgtacagttg
     7141 gtgccggtgt cgttacagaa atcaaagctt aattcactta agttttaaag taaagcaaca
     7201 tttattcgga cgtaatcgct cggaacgaag gtcttaggat ctttgttccg agctttttta
     7261 tttattgcag gatcaggaaa aaagacagga gtattataaa tagaagaaac acgaaataaa
     7321 agagtcagta ttttctaaat gaaactttat gaaaacaaaa tgttttttac aagaaaaagc
     7381 cttgctttgt gagtttatat atattataat gacaaaggtg cgtttacttt agtgaattgc
     7441 acagacgtaa tcagaatgat ttcgtcggct aagaaacgag aggttgcgac acgcccggac
     7501 gctttgccac gaacgagcgt gacggaaatt ttcgtggagc tatgtctact tttcaaaata
     7561 ggcgaaggag ggaacaagat ggcaaaacaa aaaattcgta tccgtttaaa agcgtatgaa
     7621 caccgtattt tagatcaatc agcggataaa atcgtggaaa ctgcaaaaag aactggagct
     7681 agcgtgtctg gtccaattcc attaccaact gaacgcagtc tttacacaat catccgtgcg
     7741 actcataaat acaaagattc acgcgaacaa ttcgaaatgc gtacacacaa acgtcttatc
     7801 gacattgtga acccaacacc aaaaactgtt gatgctttga tgaagctaga cttaccatct
     7861 ggtgtaaaca tcgaaatcaa attataaaac taaaacaaaa ataatggagg tgtactcatg
     7921 accaaaggaa tcttagggaa aaaagtggga atgacacaaa tcttcactga atctggtgaa
     7981 ttaattccag taactgttgt agaagctact ccaaacgtag tactacaagt gaaaacaatg
     8041 gaaacggatg gctacgaagc tattcaagtt ggttaccaag atatgcgtga agttttatca
     8101 aacaaacctg cgaaaggtca tgttgcaaaa gcaaacacgg ctcctaagcg cttcattcgt
     8161 gaattcaaaa atgttgagct aggagaatat gaagtaggaa aagaaattac agtagatgtt
     8221 ttccaagcag gagacattat tgatgtaaca ggtactacga aaggtaaagg attccaaggg
     8281 gttatcaaac gccacggaca aagccgcgga ccaatggctc acggttctcg ttaccatcgt
     8341 cgtcctgggt caatgggtcc agttgcacct aaccgtgtat ttaaaaacaa acgtcttgct
     8401 ggccgtatgg gtggcaaccg cgtaactatc caaaacttgg aagttgtacg tgtcgatgca
     8461 gaaagaaatg ttatcttgat caaaggtaat gttcctggag ctaaaaaatc tttaattact
     8521 atcaaatcag ctgtgaaagc taaataattg taggaagaaa ggaggaacta aggaatgccg
     8581 aatgtagcat tattcaaaca agatggaagc caaaacggtg aaatcacact taacgaagaa
     8641 atcttcggaa ttgaacctaa tgaaagtgtt gtttacgatg caatcgtaat gcaacgtgcg
     8701 tcattaagac aaggaacaca cgcggttaaa aaccgtagcg ctgttcgcgg cggcggacgt
     8761 aaaccatggc gtcaaaaagg aacaggtcgt gcacgtcaag gatctatccg ctcaccacaa
     8821 tggcgtggag gtggcgtagt ctttggacca acaccacgtt cttacagcta caaacttcct
     8881 aaaaaagttc gtcgtttagc aatgaaatct gtattgtcag aaaaagttgc tgaaaacaac
     8941 ttggtagcaa tcgaaggctt gaacttcgac gcaccaaaaa caaaagaatt caaacaagtt
     9001 cttgctaact tatctatcga ctctaaagta ttggttgttc tagaaaaagg taacgacttt
     9061 gctgctttat cagcacgtaa cttaccaaac gtttctgtag taacttctga taatgtgagt
     9121 gtgctagacg ttgtatcaaa tacaaaagtg ttagcaacac aaactgctct tactcaaatt
     9181 gaggaggtgc ttgcataatg aacttactag acgtaatcaa acgcccagtg atcactgaaa
     9241 aatcaatgct tgccatggat gacaagaaat acacttttga agtggacact cgcgcaaaca
     9301 aaactttagt aaaacaagcg gtagaagctg cttttgacgt taaagtcaaa aacgtgaaca
     9361 tcgtgaacgt acgtgcgaaa ttcaaacgta tgggcaaata tgcaggatat acaaaaaaac
     9421 gtcgcaaagc gatcgttaca ttgactgaag attcaaaaga aatccaatta ttcgaagctg
     9481 ctgaataagc actaatagat aactcattaa attaggaggg aaaaacgtgg cgattaaaaa
     9541 gtacaaacct acctcaaatg gtcgtcgtaa catgacaggt tctgattttg ctgaaatcac
     9601 aacatcaaca cctgaaaaat cattgttaca accattgaaa aaccgtgctg gacgtaacaa
     9661 caacggccgc atcactgtac gtcatcaagg tggcggacac aaacgccaat atcgcgtgat
     9721 cgacttcaaa cgtaacaaag ataacgttgt tgcaactgtt caaacgatcg aatatgatcc
     9781 aaaccgttca gctaacatcg cgttagtaca ctatgaagat ggagtgaaag cttacatctt
     9841 agcacctaaa ggactagaag tgggtatgaa acttgtttca ggtccagaag cagatatcaa
     9901 agtcggaaat gctcttccat tagaaaacat tccagttggt actgttatcc acaacattga
     9961 aatgaaacct ggtaaaggtg gacaattgat ccgttctgct ggtacaagtg ctcaagtact
    10021 tggtaaagaa ggcaaatacg tattgatccg cttgaactct ggtgaagttc gtatgatctt
    10081 agctgcatgc cgtgctacaa tcggttctgt aggtaacgaa caacacgaat taatcaacat
    10141 cggtaaagct ggacgctctc gttggatgcg taaacgccca actgtacgtg gtagcgtaat
    10201 gaaccctaat gatcacccac acggtggtgg tgaaggtaaa gctcctatcg gacgtaaagc
    10261 accagtttct ccttggggtc aaccagctct tggttacaaa actcgtaaca aaaaagctaa
    10321 atcagacaaa cttattgttc gtcgtcgcaa aaataaataa ttactttgac catgtgtcgc
    10381 acattttgag aggaggttca ccatgggtcg tagtttaaag aaaggacctt ttgtcgatga
    10441 gcatttaatg aaaaaagttg aagctcaagc aggggttgaa aagaaaaaag ttatcaaaac
    10501 ttggtcacgc cgttcaacag ttttcccaag ttttgttgga tataccatcg cagtttatga
    10561 tggacgtaaa catgtaccag tttacatcca agaagacatg gtaggacaca aattaggtga
    10621 atttgcacct acaagaactt atcgtggaca tgctgctgac gataagaaaa cgaaacgcta
    10681 atttgagggg aggataaatc caaatggcag aacaaatcac atcagctaaa gcaactgcaa
    10741 aaacagttcg cacttcacct cgaaaagccc gtttagtaat tgaccttatc agaggtaaaa
    10801 gtgtcgcaga tgcaatttca atcttgaaat tcacaccaaa caaatctgct ggaatcattg
    10861 aaaaagtatt gatgtcagca gttgcgaatg cagaaaataa ctttgactta gatgttgaaa
    10921 acttggtagt aagcgaagct tttgttaacg aaggacctac aatgaaacgt ttccgtcctc
    10981 gtgcaaaagg atcagcttct ccaatcaaca aacgtacaag tcatatcacg gtagtcgtat
    11041 cagaaaaata aggagggata atcagtgggt caaaaagtac atccaattgg aatgcgtgta
    11101 ggcatcatcc gcgactggga tgctaaatgg tatgctgaaa aagagtatgc tgagttctta
    11161 cacgaagatt taagaatccg taaatttatt tcaactaaac ttgctgatgc tgctgtatca
    11221 acagttgaaa tcgaacgcgc aacaggtcgc gtaaacattt caattcacac agctaaacca
    11281 ggtatggtta ttggtaaagg cggttctgaa gtcgaaagcc taagaaaaga attaaacaaa
    11341 ttaactggta aaagaatcca tatcaatatc gtggaaatca aaaaaccaga tctagatgca
    11401 aaattagtag gtgaaggaat cgcacgtcaa ttagaaaacc gtgttgcttt ccgtcgcgct
    11461 caaaaacaag ccatccaacg ctcaatgcgt gctggcgctc aagggatcaa aactcaagta
    11521 tctggtcgtc taaacggagc agatatcgct cgttcagaag gatactcaga aggaactgtt
    11581 cctcttcata ctttacgtgc ggacattgat tacgcatggg aagaagcaga tacaacatac
    11641 ggaaaactag gagttaaagt gtggatctat cgtggagaaa ttcttccaac taaaaaaaac
    11701 actgagaaag gagggaaata atcatgttag tacctaaacg tgtaaaacac cgtcgtgaat
    11761 tccgcggaaa aatgcgcggg gaagctaaag gcggaaaaga agtagcattc ggtgaatacg
    11821 gtttgcaagc tgttgattca cattggatca caaaccgcca aatcgaagct gctcgtatcg
    11881 caatgactcg ttacatgaaa cgtggtggga aagtatggat taaaattttc cctcacaaat
    11941 cttatactgc caaagcaatt ggggtacgta tgggttctgg taaaggggca cctgaaggat
    12001 gggttgcacc agtaaaacgt ggtaaaatca tgtttgaaat cgcaggcgtt cctgaagaag
    12061 tagctcgtga agcgttacgt ctagcttctc acaaattacc aatgaaaact aagatcgtaa
    12121 aacgtgagga aatgggtggt gaatcgaatg aaggttaaag aaatcagaga attaaccact
    12181 gccgaaatgc ttgatcaaga aaaacaatta aaagaagaat tgtttaatct tagattccaa
    12241 ttagcaacag gtcaattaga aaacactgca cgtattaaag aagtacgtaa atcgattgca
    12301 cgcatcaaaa ctgtgttgcg tgaacaagct aagtaattat ggaaggaggc cattaagcgt
    12361 atgactgaag gaagaaatca acgcaaagtt tatcaaggtc gcgtggtttc agacaaaatg
    12421 gataaaacaa ttaccgttgc tgtcgaaaca aagaaaaacc accctatcta tggcaaacgc
    12481 atgaactatt ctaagaaata caaagtgcat gatgaaaaca acacagcaaa agttggcgac
    12541 atcgtgagaa ttatggaaac tcgtccatta tcagctacaa aacgtttccg tttgttggaa
    12601 gtagtcgaag aagcagttat tatctaataa cacgagattt tcctatatag attgaatgca
    12661 aaatctgaaa ggaggataca caacgtgatc caagcagaaa gtcgtttaaa agtcgctgat
    12721 aactcaggcg cacgtgaaat tttgacgatc aaagtccttg gtggatctgg tcgtaaaact
    12781 gctaacatcg gtgatgtgat cgttgctacg gttaaacaag caacgccagg tggggttgtc
    12841 aaaaaaggtg acgtcgttaa agccgttatc gttcgaacaa aatcaggagc tcgtcgtaca
    12901 gacggttcat atattaaatt tgatgaaaat gctgcagtga ttattcgtga tgataaaagc
    12961 cctcgtggaa cacgtatctt tgggccagtt gcacgtgaac tacgtgaaaa caacttcatg
    13021 aagatcgttt cactagcacc agaagtttta taatcttgag acacttatca aaggaggtgc
    13081 gaaacagtta tgtttgttaa aaaaggcgat aaagttaaag ttatcactgg aaaagacaaa
    13141 aacaaagaag gcgttgtact agcagcgttc ccgaaaaaag ataaagttat tgtcgaaggt
    13201 gtaaatgtcg ttaaaaaaca ccaaaaacct tctcaagctg ctcctcaagg tggaattgtt
    13261 gagatggaag cgccaattca tgtttctaac gtgatggtgg ttgactctac tggtgtagct
    13321 ggccgagttg gctacaaaga agtagacggc aaaaaagtcc gtgtttctaa aaaaaccggt
    13381 gaagttttag ataaataaaa gtttaaggaa ggagggtctt actgaatgaa ccgcctaaaa
    13441 gaaaaatatc ttaaagaagt aactccatca ttgatggaaa aatttaacta tagctctgtt
    13501 atgcaaacac ctaaagttga aaaaatcgtc atcaacatgg gtgttggtga tgcggtatca
    13561 aacgctaaaa acttagataa agcagttgaa gaattaactt tgatcactgg tcaaaaacca
    13621 atgattacaa aagctaaaaa atcaatcgct ggattccgtt tacgtgaagg aatgccaatt
    13681 ggtgcaaaag ttacattgcg cggagaaaga atgtacgaat tcttagacaa attggtttct
    13741 gtttcactac cacgtgtacg tgacttccat ggtgtaagta aaaaagcctt tgacggtcgt
    13801 ggaaactata ctttaggtat taaagaacaa ttgatcttcc cagaagtaga ttatgattta
    13861 gtagataaag tacgtggtat ggacatcgtt attgtaacaa cagcaaactc tgatgaagaa
    13921 tctcgtgagt tgttgacaca attaggcatg ccattccaaa aataattaaa ggaggcgaac
    13981 tacgtggcta aaaaatcaat gattgctaaa aacaaacgtc ctgcaaaatt ttcaacacaa
    14041 gcttatactc gttgcgaacg ttgcggacgt ccacattcag tttatcgtaa gttccatctt
    14101 tgccgtattt gcttccgcga acttgcctat aaaggtcaaa ttcccggcgt gaagaaagct
    14161 agctggtaaa aagtaaattc gcataagaag gaggtaaaag atcaatggtc atgacagatc
    14221 caattgcaga ttttctaact cgtatccgta acgcaaacat ggttaaacat gagtctttag
    14281 aagtacctgc atcaaaaatt aaacgtgaca tcgctgaaat cttgaaacgt gaaggtttcg
    14341 ttcgtgatgt tgaatacatc gaagatgaca aacaaggcgt gatccgtgtt ttcctaaaat
    14401 ttggtaaaaa cggcgaacgt gttatcacaa acttaaaacg tatctctaaa ccaggtttgc
    14461 gtgcttatgt caaagctgac gaagtaccaa aagtattgaa cggtttaggt atcgcaatca
    14521 tttcgacttc tgaaggtgtt gtcactgata aagaagctcg tgaaaaaaat atcggcggcg
    14581 aagttatcgc ttacgtatgg taatcaagta gaatctacaa ggaggtgtct ttaagtgagc
    14641 cgtatcggaa ataaagtcgt tgttatccct gaaggagtaa cagttactca agacggaaac
    14701 aacgttacag ttaaaggacc aaaaggtgaa ttgactcgta ctttttcagc agatatcaaa
    14761 atgaatatcg aaggaaacga agtaactttc actcgtccaa acgacagtaa agaaatgaaa
    14821 acaattcatg gaacaactcg tgcgaacttc aacaacatgg ttgtaggtgt cagcgaaggt
    14881 ttccaaaaag cgttagaact aatcggggtt gggtaccgtg cacaattaca aggatcaaaa
    14941 cttgtattga acgttggtta ctcacatcca gtagaaatca caccaccagc tggagtaact
    15001 gttgaagtac cagctaacac acaagtaatt gttaaaggtg cgaacaaaga agaagttggc
    15061 gaattagctg caaacattcg tggaactcgt cctccagaac cttataaagg taaaggtatt
    15121 cgttatgttg gcgaatatgt acgccgtaaa gaaggtaaaa ctggtaaata atcgtttaga
    15181 aaaattgcat agaagccagc agcaggaaaa aggcatttgg ctgaagttta ccttcaaaaa
    15241 aatacatttt tacttgtgcc gcagcttcta tttttcgaaa cgtatttact gcttgaaata
    15301 gtagtacaat aaaaataaag aggtgacaat tgtgattaca aaaccagata agaacaaaac
    15361 acgtcaaaag agacatcgtc gtgtacgtaa caatatctct ggtactgctg agcgcccacg
    15421 cttgaacgtt tttcgttcta acaaaaacat ctacgctcaa attattgatg acgtagcggg
    15481 tgtaacgcta gcaagtgcct ctaccttgga taaagaaatt tcaggtggaa caaaaacaga
    15541 agcagcacaa gctgtcggta aactagttgc tgaacgtgca gctgaaaaag gcattaaagt
    15601 agtagttttt gaccgtggtg gataccttta ccatggccgt gtgcaagctt tagctgaagc
    15661 tgctcgcgaa aatggactag aattttagaa gaaggaggaa taccattcat ggtttatatc
    15721 gatccaaaac atttggaatt agaagaccgc gttgttgcaa ttaaccgtgt aacaaaagtt
    15781 gttaaaggtg gacgtcgtct acgttttgct gctttagttg ttgtcggtga caaaaacgga
    15841 cacgtaggat ttggtacagg taaagctcaa gaagttcctg aagcaatccg taaagcaatt
    15901 gaagatgcga agaaaaactt agttgaagta cctatggttg gttcaacaat cccacacgaa
    15961 gtaatcggtg cttttagtgg tggtagaatc ctaatgaaac ctgctgtaga aggttctggg
    16021 gttgctgctg gtggaccagt tcgtgccgta ttagaattag ctggggtagc agatatcact
    16081 tctaaatctt taggctcaaa tacaccaatc aacgttgttc gcgcaacagt tgaaggtttg
    16141 aaacaattga aacgtgcaga agaagttgca gaacttcgcg gcaaatcagt tgaagaatta
    16201 atcggataag gaggacaaca ataatggctg aattaaaagt tactttaaaa cgcagtatta
    16261 tcggacgtcc tcaaaaccaa cgcgatacag ttaaagcgtt aggtcttaca aagattaaca
    16321 gttcagtggt taagccttct aatgaagcaa ttaaaggcat gatcaatact gtttctcacc
    16381 tagtggacgt tgaagaaatt taatttagaa aaatcataac tagaacatta aggaggtgcc
    16441 aaacctatga aacttcatga attaaaacct gctgaaggtt cacgccaagt acgtaaccgt
    16501 gttggtcgtg gtacttcatc tggtaacgga aaaactgccg gacgcggaca aaaaggacaa
    16561 aaagctcgtt caggcggcgg tgtgcgttta ggatttgaag ggggacaaac accattgttc
    16621 cgtcgtttac caaaacgagg attcacaaac gtgaaccgta aagactatgc agtcattaat
    16681 cttgatgtat tgaaccgctt tgaagacgga gctgaagtaa ctcctgttgc tttagttgaa
    16741 gctggaatcg tgaaaaacga aaaagctgga atcaaagtat tagctaatgg ggaactaaac
    16801 aagaaattaa ctgtgaaagc agcaaaattc tctaaagcag ctcaagaagc aattgaagcg
    16861 gctggtggat caatcgaggt gatctaatgt ttagactatt aaaagatgct tttaaagtaa
    16921 aagacattag atcgaaaatc ctatttactg tatttgtgtt gtttgtattt cgcctaggtg
    16981 cacatattac tgtaccaggc gtaaatgctg cttcattgca aaacctttct tctttgccat
    17041 ttttcaacat gttgaacttg gtaagtggta gtgcgatgca aaacttttcc attttctcaa
    17101 tgggggtttc accatacatt actgcttcta tcgtcattca gcttcttcaa atggacatcg
    17161 tgcctcgatt tgttgaatgg tcgaaacaag gggaagttgg tcgtaaaaaa ctgaatcaag
    17221 cgactcgata tctgacatta gtattggcat ttatccaatc tgtcggtatc actgctggat
    17281 tcaatcaata cgctcaatta ggtttcgtga ataatcctaa cgtagcaact tacgtaacga
    17341 tcggtttgat tctaacagcc ggtacgatgt ttgttacttg gctaggagaa cagatcacag
    17401 aaaaaggtat cggaaatggc gtatcaatga tcatttttgc aggtatcatt tctagattgc
    17461 ctgaaagtat cagagaaatt tatgaagatt actttataaa tattgatcct tcagacatat
    17521 ggaaatctgc tatttttgtg gccattttac tagtagcggt cttgttgatc gtgacattcg
    17581 taacattttt ccaacaagct gaacgcaaga tcccgatcca atatacgaaa cgcgtagctg
    17641 gcgcgcctac aagcagctat ctgccgttga aagtaaatgc agctggagtt attccagtta
    17701 tctttgctag ttcgtttatt gctacgccaa atgctgtttt acaagcattg agcggtagat
    17761 tcggttctga aaactggtac aaagtgatga tggagatctt taactataat acagcacctg
    17821 gtgctacgat ttatacgatt ctgatcgttg ccttcacttt cttctacgcc tttgttcaag
    17881 taaaccctga gaaattagcg gaaaacttgc aaaagcaagg aagttatata ccaagcgtac
    17941 gccctggtaa ggggactgaa gagtatgtct caagattact gatgcgttta agtacagttg
    18001 gtgcagtctt ccttggttta gtggcattgc ttccaatcat tgctcaaatg gtttgggatc
    18061 ttccacaatc aatcggatta ggcggtacaa gcttactgat cgttatcggt gtagctttag
    18121 aaactgcgaa acagttagaa ggcttaatgc tgaaacgtaa atatgttgga ttcattgagt
    18181 aagaaagcgt gttgaggttt cccctcaaca cacgttctca cacatttatt aggagggaaa
    18241 acaatgaacc tcattttaat ggggctgcca ggcgcaggta aaggaacaca agctgaaaaa
    18301 atcattgatg cttatggtat tccgcacatc tcaacaggag atatgttccg cgcagcgatg
    18361 caaaacgaaa cagctcttgg cctggaagca aagtcttata tggataaagg tgcattagtt
    18421 cctgatgaag taacaaatgg aatcgtgaaa gagcgactag ctgaaccaga tacagagaaa
    18481 ggcttcttat tagatggctt tccacgtacg ctagatcaag caaaagctct ggacgcaatg
    18541 ctaaaagatt tgaataaaaa aattgatgct gtcatcgata tccatgttgg tgaagaaatc
    18601 ttggtcgaac gtctagccgg ccgatttatc tgcagcaatt gtggagcaac ttaccataaa
    18661 attttcaacc ctacaaaagt tgaagacaca tgtgatcgtt gcggtgggca tgaattctat
    18721 caaagagaag atgataaacc tgagacggtt aaaaatcgtc tggctatcaa catcaaaaat
    18781 agtgaaccaa tcttggctta ttataaagaa caaggtttgc taaacactat tgatggtgct
    18841 cgtgaaatcg atgctgtatt tgcagatgta caaaaaatca ttgaaaaata gcacgattgc
    18901 ccgtagccga cttgcattat gcagttactc ttgtcaagtt caataaatta tgatagaata
    18961 tgtcagtccg acttctagag aatcctctag atgatccatt ttgagaattt tgaggtggga
    19021 actgcagttt ccgccatttt atcgtgtgaa aatgcgaaac aagtacaagg aggtactgtg
    19081 cgtggcaaaa gaagatatga ttgaagtcga aggtacagtc gtcgaaactt tgccgaatgc
    19141 aatgtttaaa gttgaactag aaaacggaca ccaagttctt gctactgttt caggtaaaat
    19201 tcgtatgcat tacatccgta tcttacctgg tgacaaagta acggttgaat tgtctccata
    19261 cgatcttaac cgtggccgta ttacttatcg ctttaaataa ttgtactccg taaaactaca
    19321 ggaggtatta tcatgaaagt aagaccatca gtaaaaccaa tgtgcgaaca ttgtaaagta
    19381 attcgtcgta aaggacgcgt tatggtgatt tgcccagcaa atccaaaaca taaacaacgt
    19441 caaggataat tggaggtgct ataaatatgg ctcgtattgc aggagtagat attcctcgtg
    19501 ataaacgtgt agtagtttca cttacttata tctatggtat tggtaacact actgctaaaa
    19561 agatcttagc tgatgctggt gtatctgaag atgttcgcgt tcgtgaatta acgaatgaac
    19621 aaacagatgc tatccgtgca gaagtagata aattaaaagt tgaaggggat cttcgtcgtg
    19681 aagtgaactt gaacatcaaa cgtttgatgg aaatcggttc ttaccgtgga atccgtcatc
    19741 gccgtggatt gcctgttcgt ggacaaaaca cgaaaaacaa tgcacgtact cgtaaaggcc
    19801 cagctcgcgc tatcgctggc aagaaaaaat aattcttaag tgaaggaggt taaaacttca
    19861 tggcagcaaa aaaagtgaat cgtaaacgcc gtgtgaagaa gaatatagaa gcaggtattg
    19921 cacatatcca ttctacattc aataacacta tcatcatgat cactgatact cacggaaatg
    19981 cattagcgtg gtcatcagct ggttcattag gtttcaaagg aagcaaaaaa tcaactcctt
    20041 ttgccgctca aatggcagca gaaactgcta caaaagcagc aatggaacac ggattaaaga
    20101 ctgttgaagt aacagttaaa ggacctggtt ctggacgtga agcagcaatt cgttcattac
    20161 aagctacagg tttagaagtg actgcaattc gtgacgtgac tccagttcct cataatggtg
    20221 cccgccctcc aaaacgccgt cgtgtttaat gagtgccgct cattttgagt tttatcagta
    20281 acgtcattga acaagacact tttcgttttg aaaggggtaa agataagaat gattgagttc
    20341 gaaaaaccaa gaatcgcaaa aattgatgaa gaaaaagatt atggcaagtt catcgttgaa
    20401 cctcttgaaa gagggtatgg gactacttta gggaattccc tacgtcgtat tttattatct
    20461 tctttgccag gtgctgccat cactaatatt caaatcgatg gtgtactgca tgaattctct
    20521 accatcaaag gtgttagaga agacgtcact caaatcatct tgaacattaa aggcttagcg
    20581 ctgaaaatgt atggccaaga agaaaaaacc cttgaaatcg atattaccgg acctgctaca
    20641 gtaactgctg gcgatattat cgttgatagt gatgtagaaa ttcttaacaa agatatgtac
    20701 atctgtagtg tctctgaagg agctacgttc catgctcgct taacagtgaa acctggacgt
    20761 ggttatgttc aagcagatga aaataaaaaa gaagatatgc caattggcgt tcttccagtc
    20821 gattcaatct acacacctgt tcgtcgtgtg aactatcaag ttgagaacac acgtgttgga
    20881 caccgtgaag attttgataa attgacgatg gagatttgga cagatggttc aattgaaccg
    20941 ttggaagcaa tgagtttgtc tgcgaaaatc atgactgaac atttggatat ttttgtgaac
    21001 ttaactgatg aagcaaaaaa tgctgaaatc atggtcgaaa aagaagaaac acaaaaagaa
    21061 aaaatgttgg aaatgacgat cgaagagtta gacttgtctg ttcgctctta caattgtttg
    21121 aaacgtgcag gaatcaatac tgtacaagaa cttacaaata aatctgagcc tgaaatgatc
    21181 aaagtccgta acttaggacg taaatcatta gaagaagtta aagcaaaatt gcacgactta
    21241 ggtttaggtt tacgtaaaga agattaaaac cttttacgaa ggagggaaac caacgtgagt
    21301 tatcgtaaac taggacgcac atctagccaa cgtaaagcga tgttgcgtga tttaacaact
    21361 gatttattaa tcaacgaacg tattgtgact actgaagctc gtgcgaaaga agttcgctca
    21421 actgcagaaa aaatgatcac tttaggtaaa cgcggagatt tgcatgcacg tcgtcaagct
    21481 gcggctttcg tacgtaatga agttgccagc gtgcgtgaag aaaatgagga catcgtcatt
    21541 gaatcagctt tacaaaaact gttcaacgat attgcacctc gttacgcaga acgtcaaggc
    21601 ggatacacac gcattttgaa gacagaacca agacgcggtg atggtgcacc aatggttgtc
    21661 ttagaacttg tctaaaatca cactaataac atcactttat agatgattct tttagagtgt
    21721 tatgatgttg gaagatattt ttccgagtct agctcagcgc tgctcctcaa gcgaatatcg
    21781 cttggggagt aagcactcat cataaagtgt ttttttatac cttcaattac ctgtcatgac
    21841 agatatgttt gaaggcataa aaaaacattt taagcgagaa ataattgaaa attttgtctt
    21901 tttttgatag tgttagtagg aactttagga tgaagaagga gtaatcatgg agccaattat
    21961 tgaattagaa aagataaatt ataaatacca gccagatgat cttcgccccg cattgaaaga
    22021 tgtctccttt acaattgata aaggtgagtg gatcgctatt attggacaca atggttcagg
    22081 aaaatcgaca cttgccaaaa cgatcaatgg attgcttctg ccagaatcag ggatcgtgaa
    22141 agtgggaaat caaatattgg atgaagaaaa tatttggacg atccgacaga tggttggaat
    22201 ggtttttcaa aatccagaca atcagtttgt tggttcgaca gtagaagatg acgttgcttt
    22261 tggtcttgaa aatcaaggga ttcccagaga agaaatgcta gtgcgtgtga aagatgcact
    22321 tgagaaagtt agaatggctg agtttgcttc acgcgagcct gctcgtttat caggagggca
    22381 aaaacaacgt gttgccattg ccggcgtggt tgccttacga cctgatatca ttattttaga
    22441 tgaagcaacc agtatgctag atccagaagg tcgtgaagaa gtgatctcta cgatcaagaa
    22501 gatcaaagaa gaaagtcaat tgaccgttat atctatcact catgatattg atgaagcagc
    22561 taatgccaat cggatactag tgatgagaca aggcgaactt gtccgtgaag gtacaccaaa
    22621 agaaattttt tctgctggtc ctgaactgat cgacttaggt ctagatctac catttcctga
    22681 aaaactgaag agcgcactga aagaacgtgg ggttgatgtg cccagtgaat atatgacaga
    22741 agaaaggatg gtggattggt tatggacatc cgttttgaac aagtagactt cacttatcag
    22801 ccgaatacac catttgaaca aagagcctta tttgacatca atatgacgat caaagaaaac
    22861 agttatactg cattagtagg gcataccgga agcgggaaat caaccttact tcagcatttg
    22921 aatgcgcttg ttaagccaac aagcggtaca gtccatattg gagaacgaga tattcagccg
    22981 gatacggata ataagaattt aaagcccatc cgaaaaaaag ttggcatcgt ctttcagttt
    23041 ccagaagcac agctattcga agaaacggta gcaaaagaca ttgctttcgg tcctaaaaac
    23101 tttggtgtca gcgaagaaga agcactagtc ctagcaaaag aaacattaga acaagttggg
    23161 ctggatgaaa gctatttgga acgttcgcca tttgaacttt caggagggca aatgcgtaga
    23221 gtagcaatcg ctggtgtgct tgccatgagg ccggaagtac ttgtattgga tgaaccaacg
    23281 gcaggacttg atccccaggg cagaaaagaa atgatggaga tgttttggcg tcttcataag
    23341 gaacagcaga tcactattgt attagttacg catttaatgg atgatgtagc caattttgca
    23401 gattatgtct atgtgttgga aaagggacga atcgttaata gcggggagcc gcaagaggtc
    23461 tttcaaaaca ttgaatggct aaaagaaaaa caactaggcg tacctactgc caccgagttt
    23521 gcagaagaat taatggctaa agggatgaac tttgctacct tgcctttgac agctgaagaa
    23581 ttagcagacg ctatcgtcca ttatgctgga ggaaatgccc atgatgaata aattgatatt
    23641 tggtcgttat atgcctggag attccttgat ccatcgattg gatccccgag ctaaattatt
    23701 agccagtttt tattttatcg gaatcatttt tttagcaaat aattgggaaa cgtacctttt
    23761 gttggcattt ttcacattac tttctgtctt cttgtcaaaa gtagatttga aattttttat
    23821 ccgaggcatt cgcccattga tctggctaat cctcttcact gtagctttgc aggtcttgtt
    23881 tacaagtggc ggagaaactt attggagttg gggtgttttc catatcactg aatttggtgt
    23941 gatgaacggc ctctttattt tctgtcgatt cgtgttgatc atttttatgt cgactctact
    24001 cacgcttact acaccgcctt tggaattatc ggatgcgatt gagtacttgc ttcgtccatt
    24061 gaaagccgta cggttcccag tccacgaaat ttcattgatg ctgtcgattg ctcttcgttt
    24121 tgtgcctaca ttgatggacg aaacagaaaa gatcatgaac gctcagcgcg ctcgcggtgt
    24181 tgattttggt gaaggaaacc tagttcagaa aatgaaagca gtcattcctt tgctgattcc
    24241 tttatttgtc agcagcttca accgtgcgga agatcttgcg actgctatgg aagcaagagg
    24301 ctacaaaggt ggggaaggaa gaacgaaata tcgtgtattg cattggcata atagagacac
    24361 gatggttatc attgcttttg tattattaac tgtgctactg atctttttaa gaggataaac
    24421 gttcagtggg ccgctatcta ttcttttctt gctgatctgt ttgcaagaaa ggatgcaagc
    24481 agtgtatgac aagaaatatt agggtctgtg gcagactaag gtcacaggcc tctttctttt
    24541 atctgaaaag aaagaacgaa aaaattttat agaaaaatca ttattccaga taacctttta
    24601 ggaacaaagg agagaaaaac atggtacgtt acaaagcaat cattgcgtac gacggaacaa
    24661 attttaatgg attccaaaaa cagccaaatg ggcgtactgt tcaagaagaa gtagaaaaaa
    24721 cattacaaaa aatggcaaat ggaaaagaaa tcacggtctt tggttcggga aggacagatg
    24781 ccggagtaca tgcgatcggg caagtaatcc attttgatta tccagaggag cgtccgttag
    24841 aacgaatgcg tttcgcgcta gatacccagt caccagagga tattgctgta agacaagtag
    24901 aaattgtttc agaagacttc cacgcaagat atttggtcaa agaaaagacg tatcaatttc
    24961 gagtggatat agggaaacca cgcagtcctt ttcgacgcca ttatgcaagt tattttcctt
    25021 acccacttga tttggaaaaa attcagcggg cgcttcctga tttattaggg actcacgatt
    25081 tcacctcttt ttgtgcatca ggaagctcga tagaggataa aatacgtacg atctacgagg
    25141 ctaagatgga ggtaaatgaa acaaaagacg aattgttgtt tacatttagg ggaaatggct
    25201 tcttgtataa gatgatacgg atcttagtag gaacgctttt gaaaattggc aatggccgat
    25261 tgcctgaaga tagtatccca gatattattg cgaaaaaaga tcgtaatgca gcaggaccta
    25321 cagcacaccc tgaaggactt tatttgtatg aagtgaaata tgaataagag gttatgatag
    25381 aagtggtcag cttcaagaaa taagaaggta tttacgaaaa ttacttctca aaattttgca
    25441 aaatttcggc ttattttcga agaagctact tctggaacac cgtttaagga agaggtcgtg
    25501 aaatcagcgt tttgacctga gtattagaga aacaaacggg aaaatagctc ctcatatttt
    25561 gccgtttgtt gagctaatac cgaaggtcag ctgattgaac accgtttaag aaagaggtcg
    25621 tgagcctgtc atttaactct gagtcacaaa gagcaatttc aaaaaacagc ttctcatctt
    25681 tgatgaatat cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt ttgttaccta
    25741 ttttttctgt aaccaaaaaa cgatggaacg ttgttttgat tgcccttggt gcggacagtc
    25801 tgaacaggtt ccatcgtttc tttattttct acgtcttctt ctgtgtgtgt ttcgtggttg
    25861 ttcttctatt gctggatcaa tcgtgccata aaaggggcga ttgcggtcaa ggtaccagac
    25921 ataaagaata aacaatgcaa tacataggac aaaaaggatt gcacctatcg taaatccttc
    25981 aggcaagtaa ctgaaagtga cagtatgttt acctgctgga acttttatca tcaagaatgc
    26041 gtctttgaaa ttgctgacct tgactggttt cccatcaatt ttagctttcc atcctttgtc
    26101 ataaggaata gttgtcacca acatggaatc ttcagcggta tttacagtcc cagaagcttt
    26161 tctgccagtg gcttccattt cgacaccttt ttctttgatc tgattcattg cggcttgata
    26221 ggctacaaca tctagaccaa cgactttagg ctccatgaaa cttatttctt tcgtcccata
    26281 aaaacttgca gtaaatgtca ctgttgtctc ttgaggataa tagccaagat catagtattg
    26341 accagaaatg ttgatttgcg tttttcgcga tgtaccattg acagtgatcg tcgcactaga
    26401 actttctaat tgcgcaaaat tggttgggaa caaacttaag taagcttgtg tatttgcagg
    26461 cacatggact tcccaagtga gctcttttgg ctggttatct gctgtttctg agtaagtgac
    26521 gccagtcgtt gtctttgtga tcgtcgtatt caactgattc ttcaaagtag gtgtatagaa
    26581 tgtaaaatac tgttcattta gaccagacaa tgtattgatc aagtttgtct gactggtcaa
    26641 attatctgtc actggctgct caacatcatt aacggacaac ggtgcagtaa atgctagtgg
    26701 caatgcattg acattctgat acagtgtata gtcgccgctt tgaccagctt ggcggaagcc
    26761 atatttactg aacgtgcttt cgtttttaga aatgttatat ttgattcctg taaacccatc
    26821 catcagcaaa gtattattgt tataacggat attcaaattt gtccctcttg aacggaaacc
    26881 taatttatcc aggtaagagg aagaattacg atttcttata gaagaaaaca aactgatacc
    26941 actgtaacca tagttgatcg aatcgtttgc agatactgga tccagatttt ccaagcggta
    27001 aaacgagtcg gaatcttttt ttgtttcatt tacaagagct ttcaagtcag gataaggatc
    27061 agtatataaa ctgcgggaag cataattcca atcatcaaga atgccatgga tcatactgtt
    27121 cgtattgacc agtgcttcag cagacatgat caataataac aatatactca gatgattgaa
    27181 tgaaagtttt ttatgctgat aaaatcccac agcaaaagca taaatcccta agaaaaccac
    27241 tgtcaataca aaggaagtca tcgtcacata ggtgtagctt ccttcaggtt tgatgcccca
    27301 tgccaccgca aagagagcag ctacgaccaa aatgattcct aacaacagcc cacgatcgtc
    27361 ttgttctatc ttttcccatc cttttcctgc aagcaggagc actaagaaag agaacagata
    27421 ggcataacgg aataaaaaca tattcggtgc atgcatacca tgccagaaca aattcaatgg
    27481 aacaagataa aaacttgcga taagtatggc gaaaagactg ccgtacaaaa atttattttt
    27541 caacggtact ttccgactgg caaaataaaa cagacagagg actaatggta gcagacctgc
    27601 gtaaacgaac ggaatcgatc cgtattttgt cgtgtcataa acaccaatca tatttttcat
    27661 gattagatcc agataagaag tcgcttctgt tttcaacgta gtgatctgac tcagtgcctc
    27721 gccatttgac cgaagatcca gcactgctgg caatacaatg atcattgaag cgccgcctgc
    27781 cagtaaagaa gtgataccat aaggaacaat cgttttttta tattctgacc agtttgtcaa
    27841 tagacgggcg ataaaatata agaaagaaaa tacgccaatc ataaaaccca tataaaaact
    27901 ggataaaaac aagagtaaat aactgataaa taaaagtttc ggccgtttaa gatccataac
    27961 acgatgaatc cccaaaatca ctaatggaag ataaaaaaat gcatccagcc acatgatgat
    28021 ctccgaatgt gcagtgataa atgagatcaa tgcgtaactg acgctaagtg caacatgccc
    28081 ccagcgagga atcttgaacg tacgtgcaag aaaccaaaaa gccaatccgg cacagccgac
    28141 cttaagcaaa gtcagaacat acagtgcatc tggcatcatc tggtttggaa aaaagaacac
    28201 cagtggcgtg aagatcccgc ctagatagta agaaattaaa gataaataat tcaatcctag
    28261 agaagcattc catgtataaa aaatactttg ctttccaaga agaacatttc ggaagctggc
    28321 atgaaaatta gaaaactgtg aaaaggcatc acttgccaaa atacttctgc tgcttccagg
    28381 atagattccg atacttagat aaacaagtgc taaaatcaaa aacggaatca ggaaactagc
    28441 tgctgcgtag cgtccgtttt ctctaaagaa tgactttaaa ttttttttca tatttatttt
    28501 ccaccaaatt tctatgaagt tgtattcagt ttatcatatc cttgtttttc tctgcttaaa
    28561 aagagtctag ttttcacaaa aaaataaaat ttagaataaa taacttcata atttgttcgt
    28621 ataacaaaaa taaacgcaaa aaaagggtag gaatcaaaag tcccaccctt aaaaattttt
    28681 attaaataaa aaaccgttca ctatctgaac ggtccaagta tcattcaatt aaacgcgttc
    28741 aacttttcct gatttcaaag cacgagttga cacccaaact tttttaggtt ttccgtcgac
    28801 cataacgcga actttttgta agttaggttt aacagtacgt tttgttgagt tcatagcgtg
    28861 tgaacggttg tttccacttt ttgtcttgcg acctgtaaag taacaaactt ttgccatggt
    28921 aatttcctcc tttgccttga tttggagcaa tagaataatt cagctcatac cagactaaca
    28981 tatcatatag aatgggaatt tgcaagtaca ttttcataga tacaattgac aattgagaag
    29041 aataattgta aattaaacaa ggttatacct gaaattttgc aaggtttttg agtactttgg
    29101 gtaactgcct tttatttcaa gttgcgctat gataaaatat gatagaagaa aaatgggagt
    29161 tttttacccg ttaaggaggc tcattcatgg ctgtaaaaat tcaaacgtca gctggaacca
    29221 ttgaaattac caatgatgta attgcaacag ttgttggtgg cgccgcgaca gatgtgttcg
    29281 gtgttgtagg aatggccagc aaaaatcaaa ttaaagataa tattactgag attttgcgta
    29341 aagaaaatta ctcaagagga gtagttgtcc gccaagaaga taatggtatt gctgtagatg
    29401 tttatacgat tatcagctat ggtacgaaaa tctctgaagt ttcccgcaac gtccaagaaa
    29461 aagtgaaata caatctagag gcattattag gcgtaactgc caattcagtt aatgtctttg
    29521 tacaaggtgt tcgtgtactg ccagactagg caggagactg gctggtaact cagcagtcga
    29581 tctgaaggag gattatctag gtgagtgtaa cagaaataag tgcaagccag ttccaggaaa
    29641 tggtccaggc aggtgcgaat cgtctgcaag taaatgcaga atacgtcaat tcattaaatg
    29701 tattccctgt gccagatggc gatacgggaa caaatatgaa tctatctatg actagtggag
    29761 ctaaggcagt agtagattca acctcagaaa aagtaggaga attagcagca atgctttcaa
    29821 aaggattgct gatgggtgcg cgcggaaatt ccggcgttat tttgtctcaa ttgtttcgtg
    29881 gattttctaa gcaaattcca gatgttgtct cattaaatgc ttctgatctt gctgcagcct
    29941 tcactcatgg agttgaaact gcgtataaag cagtaatgaa accagtagaa ggaacgattt
    30001 tgacagtagc tagagaagca gcgaaagctg gagaaaagaa agcaaaaagt actgatgatg
    30061 tcgttgaagt aatgacagct gttgtaaaag gagcaaagcg tgctttagct aaaactccag
    30121 acttgcttcc agtcctaaaa gaagtcggtg tcgtagacag tggagggcaa ggactgctgt
    30181 ttgtttatga gggcttttta agtgcattga atggagaatt ccaagcggat gaaacatatg
    30241 aaccttctcc agcagaaatg gatgatatgg tcaatgcaga acatcatcgc agtgtccaag
    30301 gtcaaatggc gacagaagat atccattttg gttactgtac agaaatcatg gtaaaaatcg
    30361 gagaaggacc tacagtcgat agtacattcg attacgaaga attccgtaac tatttagatg
    30421 gtataggaga ttctttactt gtggtcaatg atgacgagat catcaaagtc cacgtgcata
    30481 cagaaaaccc aggagaagtc ttgaattatg gacaaaaatt cggttcatta gtcaaaatca
    30541 aagtcgacaa catgcgcttg cagcatgaga cgattttgga acacgatgaa gaagtgaacg
    30601 agtttgcgaa aaaagaaata ccacgtatcc cgtatggtat cgtagcgatc gctgctggta
    30661 aaggcgtcca agagctgttt gaaagtttag gcgctaatta tgttatcagt ggtggacaaa
    30721 caatgaatcc aagtacggaa gacattgtta aagcgattca agaagtaaac gcagataaag
    30781 tgattgtctt gccaaacaat aagaacatct tcatggcagc tgatcaagca gcagaagtag
    30841 cagatatccc agtagcagtt gttccttcaa gaacgatttc ccaaggattg acagctatgc
    30901 tttcattcaa tgatcaagct tctttagaag aaaacaaaga aacgatgaca gaaatgttgt
    30961 ctagcgttgt gagcggtcaa gtcactcacg cgatcagaga tacctcaatt gacggtgtag
    31021 agatcaaaga aggagacttt ttgggcatga tcgatggaaa aatcctgatc tctaacccag
    31081 atatcacagc aacagcgtta agtacactag aacaaatgat caatgaggac acagagatcg
    31141 taacgatctt ggcaggtgat gaaggaacag ttgaacaagc aaatgaattt gctgagaaac
    31201 taacagaaaa acatcctgat ttggaaatcg agatccacca aggagatcag cctgtctatc
    31261 cttatctatt ttctgctgaa taatttctgt gtcagaatat ctttctaaca cagtattcat
    31321 tcataaaagg ccttcggtta gtgatcaggt atgatcgctg accaagtcct ttttttgtta
    31381 aaggagggag gaaaaattta tgcgttcttt agatgatcca atcactatgc tggcaggagt
    31441 cggagaaaaa agagcagcta gtcttgccag cttgggcatc caaacgattg aagacttact
    31501 gacttattat ccttttcgct acgaagatat tcaggaaaga aatctaaacg agatccagga
    31561 tcaagaaaag gttactttga aaggaatcgt agtctcgcct cctgtgatga atcgatttgg
    31621 ttataaaaaa agccgattgc aatttcgtat gatgcaagaa catgatgtgt tcaacgtttc
    31681 tttttttaat caaccttatc taaaagaaaa agtcatttta tcggaggaaa tcgccgtata
    31741 cggtaaatgg gatgcaaagc gtaaagcctt gaacgggatg aagattttag gttctcaaac
    31801 aatggatgac ttttcgccga tatatcacgt caacaaatca attcgtcaaa cgacattagt
    31861 tgatctaatc aaaaaagggt ttcaagaata cggcacattg attgaagaaa atctgccaaa
    31921 caaccttgtg gagaaatatc gactgcttga ccgagcttct gctgtaagaa gtatgcattt
    31981 tccaaaagac cacgaagaaa accatcaagc caaacgacgt gtcgtttttg aagagttctt
    32041 tttatttcaa atgcgtcttc aaggattgaa aaaagcagaa aaagcagaaa cgaatggttt
    32101 ggagatcctt tatgatgtcc aaaaattgaa acaatttact caaaagctac cttttgaact
    32161 gacaaatgca caaaaacgtg taacgaatga aatctgtcgg gatcttcgca gtccgcagca
    32221 tatgcagcga cttttgcagg gagatgtagg tagcggaaaa acagtggttg ccgcgattgc
    32281 gttatacgct accgttacag caggtttcca aggtgcgttg atggtgccta cagaaatctt
    32341 ggcacaacaa catatggaaa gcttgcaaca gctgtttgat cccaatgaag taaccatcgc
    32401 attgattacg gggtcaacga aagcaaaaga acgacgtgag ctccttgaaa aactagagca
    32461 aggggaaatt aacgtcgtga tcggcacaca tgcattgatc caagatggag tcgaatttca
    32521 gaatttaggt ctagttatta cggatgagca gcaccgtttt ggcgtgaacc agcggaaagt
    32581 acttcgtgaa aaaggctggc gaccagatgt cttatttatg acagcgactc cgattcctag
    32641 aacattagct attacagcat atggtgagat ggatgtatct atcattgatg aacttccagc
    32701 tggacgtatc ccgatcgaaa cacgctgggt aagaacacct caattggaca gtgtactgga
    32761 ttggacgaaa aaagaactgg caagaggaca tcaaatgtat gtcatctgtc cgctgattga
    32821 agaatcagaa gcattagatg taaaaaatgc agtggaaatc tatgaaaaac ttcgtgaact
    32881 atttgcacca aaatatgaag ttggcttgtt acatggtaaa atgaagaatc aggaaaaaga
    32941 agtaatcatg gaagccttca aagacaacca gatgcagata ttagtttcga ctactgtaat
    33001 tgaagtaggg gtaaacgtgc cgaacgcgac cgtcatgctg atcatggatg cggatcgatt
    33061 tggattggca cagctgcacc aacttcgtgg acgtgttgga cgcggaagtg atgcttccta
    33121 ctgtatctta gttgctaatc ctaaaaatga actaggtgtg gaacggatga aaatcatgac
    33181 agagaccacc aatggttttg tactaagcga aaaagatctg gaattacgtg gtcctggtga
    33241 agtatttggc ttccgtcaat ctggtctgcc tcaatttgcg attgctgact tggtgacgga
    33301 tggaaatgtt ttggaggtag ccagagaaga ggctgcagcc ctttggcaaa taaaggattg
    33361 gcagatgctt cctgagtatc agcctttagc gcaaagcctc caaatagaaa atcaggataa
    33421 tcgttatttc gattagcaga ctgtgctata ctttatactg atgacggaaa aaggagtgga
    33481 aacaatgaaa attgccgtag atgcaatggg cggagatcac gcacctcaag caattgtaga
    33541 aggcgtggct ttagcgcagc aagattttcc agaagtagaa ttcttacttt acggaaaaga
    33601 agcagaaatc aagaaatact taacaaatga aaaaaacatc acgattatcc atacagacga
    33661 aaaaatcaat agtgatgatg agccagtaaa agcaattcga cgaaaaaaga ctgcatcaat
    33721 ggtccttgct gcacaagcag tgaaaaacgg tgaagcagat gctgtatttt cagcagggaa
    33781 tacaggcgcg ctactagcag cagggttgtt catcgtagga cgtatcaaaa atatcgaacg
    33841 ccctggcttg atgtctacct tgccagtagt agggaaagaa ggtgccggtt tcgatatgct
    33901 ggatcttggc gctaacgcag aaaacaaacc agaacatttg ttgcaatacg gtattttagg
    33961 ttctttttat gcacgtaaag tccgaggtat cggacgtcct cgtgtcgcac tcttaaacaa
    34021 cggaacagaa gcaacaaaag gtagtgaagt gacgaaaaaa gcctatgaac tccttcagaa
    34081 tgaaacaagc ttgaatttta ttggaaacgt agaagctcgt gagctactca atggtgtggc
    34141 tgatgtagtg gtcacagatg gatttacagg caacgctgtt ttgaaatcta ttgaaggaac
    34201 agcaatgaat atgatgaatc tattaaaatc tgctattcta aatgaaggga tcaaaggaaa
    34261 aatgggcgct atgcttttaa aagacggctt acgttctttg aaagcagaaa tggattattc
    34321 caaacatggc ggtgccgttt tgtttggctt gaaagcacca gtcattaaaa ctcatggagc
    34381 aacaggcccc gatgctgttc gctatacgat ccgccaaatc cacacaatgc ttgagacaga
    34441 tgtagttgga caattagtcg aacaatttga aaaaagcgaa gaatagtatt tggatatctg
    34501 aagcgatccg ttccattgaa aaaaaggtgg acattcctga aaaatgccag taaaattagc
    34561 catggtgtag gtaattgaca agtgtatgat tcttggaggt gaaaaagttg actcgcgaag
    34621 aagtatttaa caaagtagcg aaaattattt caaatcattt tgagatagat accgataaag
    34681 tgacggatga attgaacatc aaagatgatt taaaagctga ttcaattagt attatggaat
    34741 tcgttttaga gcttgaagat gaattcggta cggaaatttc agatgaagac gctgaacaaa
    34801 ttgaaacagt cggtggcgca gtagattata tcagcagcca tcttaaataa tttcaaataa
    34861 aagcaaagaa ggataaaaag ctgcatgcag ctttttatcc ttcttttttt aatctttttg
    34921 acacgtaaaa gtcatttttc tgataattct ggcgataaac agaaattatg ttgcaaagta
    34981 aagcggtttt ctatataatt attaataatt cattacacaa agataagaat aacctttgtt
    35041 ataaaacgat ttattctgtg taatgtcctt ttttattaag aaaaacttcc atttaggaag
    35101 cagagagggg tattcgattg tccgataacc aattattaga agttcagaac ctacatacag
    35161 gattccgact caaagatgac tattacgatg cagtagatga cgtttcattt actttagaca
    35221 aaaacgagat tttagcaatc gttggggaat ctgggtgcgg gaaaagtacc ttggctacta
    35281 cgattatggg attacttgat cctaataaca caaaaattac aggtgagatt ttatataacg
    35341 atctgaattt aatcgacctg aacgaaacgc tctataataa aatccgcgga aatgatatcg
    35401 gaatgatttt ccaagatccg ttgtctgcat taaatcctct catgagaatc gaagaccaga
    35461 tcaaagaagg actttcctat cacacaaaga tgaatgcaga acagcgtcaa gctcgtgcac
    35521 tagaattgct agaacaagta gggattccta atcctgcgag agttggacgc caatatcccc
    35581 atgaactatc aggcggtatg agacaacgtg tcatcatcgc aatcgcaatc gcctgcaaac
    35641 ctccaatcgt cattgcagat gaaccgacga cagccctaga tgtaaccatc caagcacaga
    35701 ttcttgattt actgaaagac cttcaggaag aaacaaaaac agggatcatc ttgatcactc
    35761 atgacttagg agtcgtagca gaaatggctg atcgagtggc agtgatgtat ggcggacagt
    35821 tcgttgaagt agcaggtgta aaagaactgt ttgatcatcc taagcatcct tatacacgct
    35881 ctttactgaa ctctatacca caagaaggaa atcatgatgc agaactccat gtgatcgaag
    35941 gtgtcgttcc ttctctgaaa aacatgcctc gtaaaggttg tcggtttgca ccacgtattc
    36001 catggatacc agcaagcgat catgaagaag agcctatcct tcatgaagtc gcaccgaacc
    36061 acttggtacg ttgctcctgc tataagcatt tccactttag agacgaaaaa ggagaggtgt
    36121 agatgacaga attaatcaca atcaaagacc tgaaagttca ttaccctatt cgcagtggat
    36181 tcttcaatcg agtaacggat catgtatatg ctgtagatgg tgtggatttt attatcgaaa
    36241 aaggaaagac atatggttta gtgggagagt ctggctctgg aaaatctact acgggaaaag
    36301 ccgttgtggg attagaaaag gtaacagccg gacaaatcat gtatgaagga aaagatgtaa
    36361 cgaagcgaag caaccgcaaa aaaatgggtt ataacaagga tgtgcagatg atcttccaag
    36421 attcaatgtc cagtttgaat cctaaaaagc gagtgttgga tatcattgct gaacccatcc
    36481 gaaattttga acggttaagc gatcaagaag aaaagaaaaa ggtaaaagga ctgctagata
    36541 tcgttgggat gccggaagat gcgctatata aatatcctca tgaattttca ggcggacaac
    36601 gtcaacgtct aggagtagcc cgtgctgttg ctaccaatcc aaaactgatc gtagccgatg
    36661 aacctgtatc tgctctggat ctatctgttc aagcgcaagt attgaatttc atgaaacgaa
    36721 tccagcaaga atacggcctc agctatttat tcatttcaca tgatttaggt gtagtaaaac
    36781 acatgtgcga caatatcgca attatgtaca aaggtcgatt tgtagaaatc ggtacaagag
    36841 aagacattta taatgacccg cgacatatct atacgaaacg tctgctttca gctattccgc
    36901 aaatcgatgt agaaaatagg gaaagccaca aagaaaatcg acgaagagta gaacaagagt
    36961 atatccaaaa ccaaaaagaa tactatgatg cgactggtcg agtctacgat ttgcgtacga
    37021 tcacaactac acacaaagtg gcgttgaaag atggaggtgc aagctaatgt ggaaaacgat
    37081 tctgcgacgt gttttattga tgatcccgca agttattatt ttaagtgttc tgatcttctt
    37141 attagcccaa gcgatgccgg gggatccatt taccggtttg atcaatccaa atcaggatcc
    37201 ggcagtgatc gaacagatgc gacaagcagc tggcttgaat gacccatggt atgtccagta
    37261 cttccgttgg atcggaaatg ctttgcaagg cgattttggt caaagtttct tatacaaacg
    37321 ctctgttgct gacttgatcg gcgaacggat catcaacact ttatatctat cgatcttgac
    37381 agttgtcctt acttatttga ttgctgtgcc tctggggatg cttgctggcc ggtatcaaaa
    37441 ttcaatcttg gataagattg tcgtaatcta caacttcttt agttttgctg ttcctttatt
    37501 tatcttcggc ttgatcatgc tatttgtttt tggttatcgt ttaggctggt tcccaacaag
    37561 tggatcacaa acactaggtt tgcaaggtgg atttatcact caatggatag accgtttgaa
    37621 atatattctg ttaccatcaa ttacccaagc atttttggcg actgccgtca caatccaata
    37681 tctgcgaagt gaagtaatcg attcgaaatc gctcgatttt gtcagaacag cccgttccaa
    37741 aggggtaccg acgaataaag tattcagtcg acatattttc cgaaatgcag cgttgccgat
    37801 ggcttctcaa atggggtatg aaatcacttc gttgattgct ggttctgtag ttatcgaaaa
    37861 gatttttggt tatccgggag tcggaaaact atttattgat tccatcggcc aacgggacta
    37921 taccgtgatc acatccttag tattgatttt aggaatcgcg acgctagtgg ggacattact
    37981 ttcagatatc atcatgagta tcgttgaccc gcgtattcgg attcaataaa accaatcaga
    38041 aaggagaatg gatcgatgag tgaaaaaaac aatacagcag ttcaagaaag tattcctcct
    38101 atggggatac gaatgattgc tcgagaattt aaaaaagata aaattgctat tttttcatta
    38161 ggtttgctag tggttttatt attagtgatc tttatcggtg cgatgctgac cgatcaagac
    38221 aaagtgatgt atgttagtat cttcgacaaa tacgctgcac caggaacagt ttcgacacaa
    38281 ggacaaaagt tcattctggg agcagatgaa ggcggacgtg atgtattagg acaattgatc
    38341 ataggtgcta gaaactctgt tacgatcgga tttgcgatca cgatccttac ttcgatcatc
    38401 ggggttggat tagggatcat ctctggctac tacggaggga tcattgacaa catcttgatg
    38461 cggatcgttg attttatcat gattctaccg atcatgctga tcattatcgt cttcgtatcg
    38521 attatttcca gatacaatgt ctggtccttt atattcatca tgagtgcatt ttattgggta
    38581 gctaaagccc gattattccg aagcaagaca ttatctgaag caagaagaga ttatgtcagt
    38641 gcctcaaaaa caatgggaac caatgatttt aaaatcatgt tccgcgaaat catgccgaat
    38701 ctaagttcat taatcattac taatttgacg atgaactttg cggcaaacat cggtatcgag
    38761 acaactttga ctttcttagg tttcggcttg ccggcaaatg tacctagttt aggtacattg
    38821 atcggttatg caagtaacgg agaagtctta gtgaatcggc aatggatctg gttacccgca
    38881 tcaatcctta ttttagtttt gatgttgagt atcaattatg ttggacaagc attcaagcgt
    38941 tctgcagatg cgcgccaacg gttaggctag tatagaagcg aagggggaaa taggaatgaa
    39001 gaaaacgatt tttggatttg cagcgctgat gtctgctgtc gttcttgcag catgcggggg
    39061 cggaggtaca gatacctctg gaaacaactc tggaggcaac ggcaataaca aagaggaatt
    39121 aacttttcca attgcaacaa aaaataatga tgatgcaatc gaagacggcg aattagaagc
    39181 tgcggtcgca acggacagtc agtttaaagg attgttccag tgggagtttt accaagattc
    39241 atatgatgca gcttttatgg ctccttctca tgaaccgcta tttgcaaacg atgacgattt
    39301 cagaatcaca gacgacggag ctgcaacgct tgatctagat gaagatacga aaaaagcgac
    39361 aatcaaagta aaagacaacg tcaaatggtc agatggtgaa gatgtaacgg cggacgatgt
    39421 gattttccct tacgaaatca ttgggaataa agattacact ggtatccgtt atgacgacac
    39481 tttccgtaac atcgtaggta tggaagaata tcatgatgga agtgccgaca caatctccgg
    39541 aatcaaaaaa atagacgaca aaacagttga aatcgaatat aaagaagtaa acccaagcat
    39601 gcttcaatcg ggtggtggta tctggtcata tgcagcacca aaacatacgt tagaaggtgt
    39661 agcaatcaaa gatatggaat caagcgatca agtacgtaaa aatccagtga cttttggccc
    39721 ttattacatg agtaatatcg ttgcaggaga atcagtagaa tacttgccaa acgagcacta
    39781 ctggaatggt acgcctaaat tgaaaaagat cacgatgaaa tctgtaccga tggcaagtgc
    39841 aactgaagca ttgaactcta aattatatga tatgattttc caaatgccaa cagatactta
    39901 cgatacctat aaagaaattg ctggctatac aaacttaggc cgtgaacaaa cttcttatac
    39961 ttatctagga ttcaagttag gtaaatggaa tgcagataca aagagtgtgg aatacaatcc
    40021 agattcaaaa atggctgata aatcattacg tcaagcaatg ggctatgcat tagataataa
    40081 tgcagtaggt gaacgtttct atgcaggttt gagaagcaat gcgacttcat tgattcctcc
    40141 agtattccag tcataccatg atgatgagat gaaaggattc acacaagatc tagacaaagc
    40201 gaaaaaacta ttagatgatg ctggctacaa agatgtggat gacgatggtt taagagaaga
    40261 caaagatgga aataaactaa caatcaactt tgcttcgatg tccggtgggg aaacagcgca
    40321 accattggca gattactata tccaacaatg gaaagaaatt ggtttggatg tccaatacac
    40381 aacaggtcgt ctgatcgaat tcaattcttt ctatgataaa ttagaaaacg atgatcctga
    40441 aattgatgtt taccaagctg cttggaatac aggaactgat ccaaatccaa gtggactatg
    40501 gggaccaaat gctgcgttca actacactcg ttatgaatca gaagaaaata caaaattgat
    40561 caacgatatc agttcaagca aagcatttga tgaagagtac caaaaagaag catatcaaaa
    40621 atggcaacaa tatgcatttg aagaagcatt tgcgattcca acgttgttcc gtaatgaagt
    40681 attgccagtc aacgaccgtg tcaaagactg ggattggtct tatgatgtac caaacccatg
    40741 ggcaaatgtt tctgttacag cagattcacg ttcataatca ctaaaaatga ccgaaaaagg
    40801 gcgatgtcct ttttcggttt tttgatattt ttctgtttat ttctctaata ttcaggtcaa
    40861 ggcgctgatt tcatgacttc tccaataaac ggtgttcaaa gtccagtttc tcggtattaa
    40921 gtcaaattta tgcaaaaata tggaaagcca ttttcacaaa attttttctt catactcgaa
    40981 actaaacgaa tttttcacaa cttctcgtca atcactagga ttattaaagc tttacttacc
    41041 ttttgaccga accatttcaa atttgacaaa aaaccagtat aataaggaag tatcgaaaaa
    41101 atatccataa aaggaatgtg gaaaatggac aaccagctga caaaagaact aaaagaaaac
    41161 tataacatcg tttttcacga tctaaatttg cttgaacaag cttttaccca ttcatcctat
    41221 gtgaatgagc atcgcaatct gcaattatcg gataatgagc gacttgaatt tttaggagat
    41281 gccgttttag aactgatggt ttctcagtat ttattccgtt tatatcctca tttgccagaa
    41341 ggaaaattga caaaaacacg agctgcgatc gttcgcgaag acagcttgtc gaaatttgcc
    41401 aaagaatgtc actttgatca atatgttttg ttgggcaagg gagaagagaa ttcaggagga
    41461 cgtacgcgac cagcattgct ttgcgaccta ttcgaagctt ttttaggcgc gttatatctt
    41521 gaccagggat tcgacacgac ccatgcattc atcgaaaaag tgatttttcc taaagtcaaa
    41581 gcaggtgctt tttcacatga gatggatcat aaaacaaaac tacaagaagt cttgcaaaaa
    41641 tcaggtgatg tatcaatcga ataccgattg atcaatgaag aaggtccagc tcatgaacga
    41701 gtattttgga tcgaagttta tgtagatgat caattgattg gcactggaca agggaaatcg
    41761 aaaaaattag cagaacaagc tgcggcggaa aatgcattgg cagcactgta aaagtaagtg
    41821 agggaaagct ttgtatttga aacgaattga aatagccggt tttaaatcat ttgcagacaa
    41881 gacagtcatt gattttgaaa atagcgtcac agcagtggtt ggaccaaatg gcagcggaaa
    41941 aagcaatatt acagaggcta tccgctgggt attaggagaa caatctgcta aaagtctgcg
    42001 aggcggaaaa atgccggata tcatctttgc cggttccgat acccgtaagc agctgaatat
    42061 tgcagaagtc acagtgattt tagacaatag tgatcattat ctgccattgg agtacaatga
    42121 gatcagtgta acccgccgtt atcgacggac tggggaaagc gagtttttca tcaataaaca
    42181 aagctgcaga ttaaaagata tccaagaatt gttcttagat tctggattag gaaaagaatc
    42241 gttctcaatc atttctcaag gaaaagtaga agcgattttc agtagtaaac cggaagaccg
    42301 tcgtgggatc tttgaagaag cagctggtgt cttgaaatac aagcagcgga aaaagaaagc
    42361 agaacagaag ctttttgaaa cagaagacaa cctcagccgc gtccaagaca tcatccacga
    42421 attggaagaa cagttaactc ctttggccgc ccagagtgag gcagcaaagg agtttttacg
    42481 gctaaaagaa acactgactc aaaccgatgt ctctttgatg gttgctgaaa tcaagacagc
    42541 aaaaaaagat tgggacaaca aacaagcaca gcttgcgaaa ttcaatctag aattaggcaa
    42601 actatctgag tcgatccaag aacaagaatc cattttggcg aagcagcgta aagaaaatgc
    42661 gcaagccgat cgtctgatcg aaaaaaatca gcaagtatta ttagatcttt cggaaaaact
    42721 gaagcagaca gaaggacaaa aagatgtttt gcaggagcgg acaaagcata cacaaaaaag
    42781 ctcgcaagaa tatcaaactt cattagcaga agcacaaaaa aaggttaaac attttgaaaa
    42841 gcttcaagaa agcttgatga aagcagcagc agaaaaagaa acagagatcc aaaaagctga
    42901 agcaaatctc ataaaaacac agcaagaatt agaaaaatac caaaaatcaa cgaaagaatt
    42961 attggcagaa ttaagagatc aatatgttga tttgatgcaa gaacaagcag ctgtaggaaa
    43021 cgaactgaag tatctagaac gtcaatatat acaagaaaca gcaaaaagca agcagacgtt
    43081 agccaagcaa tcggaagtag aagcatcagt agaccgattg atcttacaaa aagaagaatt
    43141 gacacaaaag caggcgcagc taaaatcatc tttaactgaa acaaaagaga agttagaaat
    43201 gatccaacaa aacgggaaaa agttccaaga agcattagca aaagaacagc ctaaaatgta
    43261 tcaattaatg aatcaagtcc agcagctacg tgctcgtaag aaaagccttc aagaaatcca
    43321 agaaaactat tttggttttt accaaggggt tcgattggtc ttgcagcata aacagcagct
    43381 ttcgggtatt gtcggagcag tggctgaact gatcgatgtt cctgcagatt tcacgttagc
    43441 tatcgaagca gctttaggtg gggcagccca gcatgtcatc gttgaaaatg aaaaagatgc
    43501 tcggcaagcc atcacgtatt tgaaacagca aagaggagga agagcaacat tccttccact
    43561 gacgacaatc aaaccaagac aattaccggc tcatatattg acacaagcag cagctgtaga
    43621 agggtttata gggattgcca gtgaacaagt cagttatccc gatcaaatcc aaactgtggt
    43681 tcaaaacctg ctaggaacga tccttttagc caaagactta actagtgcca atgcaatcgc
    43741 gcaaacgatc cgctatcaat atcgagtggt ttccttagaa ggtgatgtga tgaatgccgg
    43801 aggatccatg acaggtggag cgaataaacg aggaaaccaa ggcagtttgt ttgtacaaaa
    43861 tcaagaattg aaacagttga ctagtgaatt tgaagaagca gataaacaac tccaagcaca
    43921 agaaaaaaaa gttcaggagc tgcaacaaga aacagctcgt ttagccgaag aacaagaagt
    43981 tttgcgtaca cgaggcgaac aattacgttt tgaagaacaa gaagcgacaa atcagctaca
    44041 aaacatcata aatgaacttg aacgttttga aaaagaaaag caaatctcaa cttttgagac
    44101 acgtgaactg cagcagttta tcgaagatta cgaaaagcaa acgaacgaat tgaaagacaa
    44161 acaaacagat cttgaatcgc aacgacaaaa aatcgacgaa gaaatcaaat cgttaagtca
    44221 agaaagcgac cagatggaag ccagaagagc acaagtacaa agtcaaaaag cacaagaaca
    44281 agcagatctt gctgtcttga aagagcagtt taatcatctg cagatccagc tccgcggtgc
    44341 ccgagtccaa aaagcagagg caaccgaacg tcaagaagcg atagaaaagc agttggcgac
    44401 attgacagca gacttttctg atcatgaagt cacagaagaa agtttggaaa aacaaattaa
    44461 cgaattatct gctcagcgag aaacattaaa agccgagttg gcaaaagcca aagagcagcg
    44521 agatagaagg caaaaggaga tcgataagct tgaagcagtc ttggcagagc gtaatcgtga
    44581 gcaaaaagca cgactgtcgg aacaatccaa attagaagtc caaaaagacc gagctgaaat
    44641 gattttagat aatcatttga cctatctgca gtcagaatat cagctgacat ttgagaaagc
    44701 ttcccaagac tatcaagaaa caaccgatat cgaagacagt agaacaaagg tttcatcctt
    44761 aaaagaacag attgaaaaac tgggaccagt aaacttaaat tcaatcgaac aatacgaaca
    44821 agtcagtgaa agacatacat tcttagcaac acagcgagat gatctgttag cagcgaaaaa
    44881 ccaattgttt gaaacgatgg atgagatgga tgatgaagtg cggacaagat tcaaagaagt
    44941 atttgaagct atccgtcaag aattcaaaat tgtctttcca aatatgtttg gcggcggtaa
    45001 agctgaatta gtactgacgg aaccaactga tttgctgaaa acaggtattg agattgaagt
    45061 acaacctcct ggcaaaaaac tgcaaagtct aagtttgctt tctggaggag aacgagcact
    45121 tacagccatc gcgttgctct tttccattat ccgtgtctgt cctgttccgt tttgtatctt
    45181 agatgaggta gaagcagcgt tagatgaagc caacgtgaaa cgttttggtc gttacttgag
    45241 cgatttccaa gatgacacac aatttatcgt agtcacacac cgcaaaggaa cgatggtcgc
    45301 agcagatgta ctgtacggag tgacgatgca ggaatccggt gtatcaaaaa tcgtttctgt
    45361 acggatggaa gatatcaatg aagaggcgaa aatcaacgaa acgaaagtgt aggaacgaaa
    45421 atgatcaaat tagtagcaat cgatttggac ggtacattat taaatgaaca aaagctgatt
    45481 tcagatgaga ataaacaagc attagcacaa gcaaaacagc aaggggtcaa gatcgttcta
    45541 tgtacaggac gccccttagc tgcgatggcg cattatctgc aagaattagg actggtagat
    45601 gaaggtgact acagcatcac tttcaacggc ggtttggtac aaaaaaatga tactggtgaa
    45661 atcatggaaa agaaagtgat ggaagtatca gatatccaac gcttatacaa actggctcag
    45721 caattggatc tgccactaga tgttttgtct gacaatgtgg ttttacagct gcctagtgcc
    45781 ccaaggaagc aatccttgta caatgtgttg aataagctgc tgcaattcca accagcggat
    45841 ataagcgata ttacagatga atttgtgctt aataaggcag taatcgctta tgaccaagaa
    45901 gaattagatc ctaaaatcaa agaaatacca gcagaattcc atgaaatgta tgagatcatc
    45961 aaaacaagaa gtatgctttt ggaatttatg ccaaaaggtg ttacaaaagc ttatggtatt
    46021 tctttattgg caaaagatct tggtttgaaa caagaagaaa tcatggcgat cggtgatgag
    46081 gaaaatgatt taccgatgat ccaatatgct ggactaggcg tagcgatgga gaatgctgtg
    46141 cctttcgtca aagaagcagc agatttcgtg acagccagca atgaagaaaa tggcgtagcc
    46201 aaagtgatcc aaaaatttgt attagagtag ggggagtcaa atgggatttt ttgataaaat
    46261 caagcgtgct tttactggcg aagagaaaac agaagaagtt gaagtacagg agaagtacga
    46321 caagggttta gaaaagacgc gtaaaacatt tggtgaacgg atgaatgagt tgtttgccaa
    46381 ttttcgaaca gtcgatgaag acttttttga agaattagaa gaaacactta tcggtgcaga
    46441 tgtaggtttt gaaacagcga tacgaatcac cgaagcatta cgtcaagaag tgaaactcag
    46501 aaatgcgaaa aaatcggcag aagtccaaaa tgcgatcatc gagaaacttg tggatctcta
    46561 tgaagcggaa ggaatcaatg aagtaaatga actaaatatc caagaagacg gactaacagt
    46621 tattttgttt gtcggtgtta atggtgtagg gaaaacaaca agtattggta aacttgccca
    46681 tcagttcaaa aacgaaggta aaaaagtatt gatggcagca gcagatacct tccgcgctgg
    46741 agcaatcgac cagttagttg tctggggcga acgcgctggt gttgaagttg ttcgtggcaa
    46801 tgcaggaggc gatccagcag ctgtcgtatt cgatgctttg gaaagagcaa agacagagca
    46861 ggcagatgtt ttactagttg atactgcagg ccgactgcaa aataaagtga acttgatgaa
    46921 cgaattagaa aaaataaaac gagtaatcca gcgggaatta ccagaagcac cccaagaaac
    46981 acttcttgta ttagatgcaa ccactggtca aaacgcgatg gttcaagcaa aacaattcaa
    47041 agaaacaaca gatgtgaccg gtcttgtttt gacaaaatta gatggtacag caaagggcgg
    47101 tatcgtactt gctatccgta atgaattgca tttaccagtg aaattggtcg gtcttggtga
    47161 aggaatcgat gacttggagc cgtttgatcc aaatgatttt gtcgttggat tgttcaaagg
    47221 attactaaaa gaagaataac ccatgtctaa aaagcaggag ctgtgacaaa a
//
LOCUS       DACECR010000011        45998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.011, whole genome shotgun sequence.
ACCESSION   DACECR010000011 DACECR010000000
VERSION     DACECR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.011"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(74..793)
                     /locus_tag="IWO17_000566"
     CDS             complement(74..793)
                     /locus_tag="IWO17_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ0289465.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            1130..1462
                     /locus_tag="IWO17_000567"
     CDS             1130..1462
                     /locus_tag="IWO17_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289466.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            1449..3452
                     /locus_tag="IWO17_000568"
     CDS             1449..3452
                     /locus_tag="IWO17_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ0289467.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            3471..3941
                     /locus_tag="IWO17_000569"
     CDS             3471..3941
                     /locus_tag="IWO17_000569"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ0289468.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            3962..4546
                     /locus_tag="IWO17_000570"
     CDS             3962..4546
                     /locus_tag="IWO17_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ0289469.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            4561..5547
                     /locus_tag="IWO17_000571"
     CDS             4561..5547
                     /locus_tag="IWO17_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ0289470.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            5537..5848
                     /locus_tag="IWO17_000572"
     CDS             5537..5848
                     /locus_tag="IWO17_000572"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ0289471.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            5870..7651
                     /locus_tag="IWO17_000573"
     CDS             5870..7651
                     /locus_tag="IWO17_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ0289472.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            7644..9020
                     /locus_tag="IWO17_000574"
     CDS             7644..9020
                     /locus_tag="IWO17_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ0289473.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            9027..9662
                     /locus_tag="IWO17_000575"
     CDS             9027..9662
                     /locus_tag="IWO17_000575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ0289474.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            9692..11047
                     /locus_tag="IWO17_000576"
     CDS             9692..11047
                     /locus_tag="IWO17_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ0289475.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(11239..12633)
                     /locus_tag="IWO17_000577"
     CDS             complement(11239..12633)
                     /locus_tag="IWO17_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0289476.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            12725..13696
                     /locus_tag="IWO17_000578"
     CDS             12725..13696
                     /locus_tag="IWO17_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ0289477.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            13693..14475
                     /locus_tag="IWO17_000579"
     CDS             13693..14475
                     /locus_tag="IWO17_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ0289478.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(14644..15015)
                     /locus_tag="IWO17_000580"
     CDS             complement(14644..15015)
                     /locus_tag="IWO17_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0289479.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            15149..15592
                     /locus_tag="IWO17_000581"
     CDS             15149..15592
                     /locus_tag="IWO17_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0289480.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(15635..16444)
                     /gene="yidA"
                     /locus_tag="IWO17_000582"
     CDS             complement(15635..16444)
                     /gene="yidA"
                     /locus_tag="IWO17_000582"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ0289481.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(16463..17830)
                     /locus_tag="IWO17_000583"
     CDS             complement(16463..17830)
                     /locus_tag="IWO17_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0289482.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(17814..18644)
                     /locus_tag="IWO17_000584"
     CDS             complement(17814..18644)
                     /locus_tag="IWO17_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ0289483.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            18807..19244
                     /locus_tag="IWO17_000585"
     CDS             18807..19244
                     /locus_tag="IWO17_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ0289484.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            19320..19940
                     /locus_tag="IWO17_000586"
     CDS             19320..19940
                     /locus_tag="IWO17_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ0289485.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            20129..20635
                     /locus_tag="IWO17_000587"
     CDS             20129..20635
                     /locus_tag="IWO17_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ0289486.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            20652..21551
                     /locus_tag="IWO17_000588"
     CDS             20652..21551
                     /locus_tag="IWO17_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ0289487.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            21638..22522
                     /locus_tag="IWO17_000589"
     CDS             21638..22522
                     /locus_tag="IWO17_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0289488.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(22714..22785)
                     /locus_tag="IWO17_000590"
     tRNA            complement(22714..22785)
                     /locus_tag="IWO17_000590"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22750..22752),aa:Arg,seq:ccg)
     gene            22845..24179
                     /gene="rpoN"
                     /locus_tag="IWO17_000591"
     CDS             22845..24179
                     /gene="rpoN"
                     /locus_tag="IWO17_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ0289489.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            24382..25419
                     /locus_tag="IWO17_000592"
     CDS             24382..25419
                     /locus_tag="IWO17_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ0289490.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            25460..26461
                     /gene="gap"
                     /locus_tag="IWO17_000593"
     CDS             25460..26461
                     /gene="gap"
                     /locus_tag="IWO17_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0289491.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            26571..27761
                     /locus_tag="IWO17_000594"
     CDS             26571..27761
                     /locus_tag="IWO17_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ0289492.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            27865..28620
                     /locus_tag="IWO17_000595"
     CDS             27865..28620
                     /locus_tag="IWO17_000595"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ0289493.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            28736..30034
                     /gene="eno"
                     /locus_tag="IWO17_000596"
     CDS             28736..30034
                     /gene="eno"
                     /locus_tag="IWO17_000596"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ0289494.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            30434..31573
                     /locus_tag="IWO17_000597"
     CDS             30434..31573
                     /locus_tag="IWO17_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ0289495.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(31640..32215)
                     /locus_tag="IWO17_000598"
     CDS             complement(31640..32215)
                     /locus_tag="IWO17_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0289496.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(32220..33194)
                     /locus_tag="IWO17_000599"
     CDS             complement(32220..33194)
                     /locus_tag="IWO17_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ0289497.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(33218..33262)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            33477..34445
                     /locus_tag="IWO17_000600"
     CDS             33477..34445
                     /locus_tag="IWO17_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0289498.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            34558..34836
                     /locus_tag="IWO17_000601"
     CDS             34558..34836
                     /locus_tag="IWO17_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0289499.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            34954..35190
                     /gene="secG"
                     /locus_tag="IWO17_000602"
     CDS             34954..35190
                     /gene="secG"
                     /locus_tag="IWO17_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ0289500.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            35302..36048
                     /locus_tag="IWO17_000603"
     CDS             35302..36048
                     /locus_tag="IWO17_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0289501.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            36051..38408
                     /gene="rnr"
                     /locus_tag="IWO17_000604"
     CDS             36051..38408
                     /gene="rnr"
                     /locus_tag="IWO17_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ0289502.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            38430..38894
                     /gene="smpB"
                     /locus_tag="IWO17_000605"
     CDS             38430..38894
                     /gene="smpB"
                     /locus_tag="IWO17_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ0289503.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            39196..39915
                     /gene="atpB"
                     /locus_tag="IWO17_000606"
     CDS             39196..39915
                     /gene="atpB"
                     /locus_tag="IWO17_000606"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ0289504.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            39968..40183
                     /gene="atpE"
                     /locus_tag="IWO17_000607"
     CDS             39968..40183
                     /gene="atpE"
                     /locus_tag="IWO17_000607"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ0289505.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            40274..40798
                     /gene="atpF"
                     /locus_tag="IWO17_000608"
     CDS             40274..40798
                     /gene="atpF"
                     /locus_tag="IWO17_000608"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ0289506.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            40785..41327
                     /locus_tag="IWO17_000609"
     CDS             40785..41327
                     /locus_tag="IWO17_000609"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ0289507.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            41350..42906
                     /locus_tag="IWO17_000610"
     CDS             41350..42906
                     /locus_tag="IWO17_000610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ0289508.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            42920..43822
                     /locus_tag="IWO17_000611"
     CDS             42920..43822
                     /locus_tag="IWO17_000611"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ0289509.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            43845..45251
                     /gene="atpD"
                     /locus_tag="IWO17_000612"
     CDS             43845..45251
                     /gene="atpD"
                     /locus_tag="IWO17_000612"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ0289510.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            45265..45687
                     /locus_tag="IWO17_000613"
     CDS             45265..45687
                     /locus_tag="IWO17_000613"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ0289511.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT        15178 a         8162 c         9869 g        12789 t
ORIGIN      
        1 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacaacccc
       61 ctcagaaagt ctattaaaat tcattcacaa agaaatcgaa taagcgctgt tcagatggat
      121 gtacacggtg ggtaagttcc ggatgccatt gaacaccgag gattttatgc atatcatctt
      181 tgctttgaac cgcttcaacg agtccatcgt tagaaaaagc gatggctttt aataaaggtg
      241 aaagatcttt gactgcctga tgatggtaag aattcacaaa atatggatcg gggagaaggt
      301 tgcttaacaa actgtctgaa acaatcttga cttcgtgagt cgtaaattgc ggggcagtgg
      361 gctgttgttc atgttttact gaccactttg gatataatga aagatcttga taaagtgtac
      421 cttccaaaac gacattcaat agttggagtc cccggcagac tgcaaaaatt ggttttcttt
      481 gtttcaatgc ttccaagatc agtgcctgtt caaattggtc tcgttgaata tttgtttccg
      541 ttagctttgg atgaggatct tccatgtaaa gttgtggact gacatcttga ccacctgcaa
      601 gaataagttt gtcgatttgt ccaatcagtt gaggagctga atctggagac atgactggca
      661 gaataagagg caatccttga gcatctaaaa ctgcatccac gaatccttgc ggtgtatagc
      721 tcacttgatt tccttggaaa gtatctgttg ctcgaatcag ttgatttccg gcaatgccga
      781 tgattggttt catttgtatt ccccctaaag tagacttatt gtaacaagga tttaaaaaaa
      841 attaaactat gtaagacttg cccctatact tatctgttct tgctgtgtta ctatctgtaa
      901 agagaaattt cgtgtgaaat tcatgacgat agttgaaaat attgcttaat ttgttataat
      961 aaaaagtaag cagaaataaa aaggttgggc gtaagcttca aggatctagt tcttcaaggg
     1021 gtgagaagga cattttattg aaggggcctg cctatactaa gttatcttta gaggtacata
     1081 ctttcaatga aaaaagaaag catgtacctc ttttttaaat cgaaaggaag tgaagcaagt
     1141 gttagaagct ttggaacaaa tccgaaaagc agaagaagaa aatgaaaagc gtgctcaaac
     1201 actgcggcag cagctgaaga tctatgcaac agaaaaagag caggaattgg aaacgatcca
     1261 aacaaactat cgacaacaac taaaacagtt ggtggaagaa aaagaacaat ttgaaataca
     1321 agaattagaa aaagaaaaaa tgaaattgaa agcagaagca gacaaggtgc aagcaacttt
     1381 gaaagatcaa tatcaaaagc atcagagtca agcgatcggt gcgatcattg aaagggtgaa
     1441 agaaacttat ggccgtcact aagatggaaa aagtgaccct catttcagat aaaagaaatc
     1501 aggagaggat tttacaagca gtacaaggaa tccaaaacgt tgaaatccgg gatctgtatc
     1561 aaaatagaac aaacaaccag tgggtggaga agtattttcc agataccacg attattgata
     1621 aagaagcaaa attaagcgct ttaggtaatc ggttgaaaga catccaagaa gctatccaat
     1681 tcatacaaca ccacggcgat agccgtcaaa agaaactcca tttgaaacgt caagaacttt
     1741 ccttacaaga tttggaagcc aattattccg aagaaaattt cataaataaa ttgaaagaag
     1801 tccttgattt aaaagaaaag tgggaacaac taactgaaga acgtgatcgt ttagaagaag
     1861 aggaagaatg gcttcttcat tggcaacagc tggatattgc tccaagtatg tatgcaagta
     1921 agacgaccat ttttgagatg gggactgtga gtgcagcaaa ttttgaaagt ttcaaggaag
     1981 aactgagtaa gcttgatgac gtttatatgg aagaaataga ttatacgcca aaacaagttt
     2041 atctttcgta tgttgcttta aaaaaatcag ttgctgttat agaagaaatt gctgggaaat
     2101 acgggttcaa taaagaaacg tatccttatg aacaaaatcc taaagaaaaa ctgcaagaga
     2161 ccaagcagca attatccatt gttcacgagc agcaaaagaa gataacgaca gcgttaggtc
     2221 agtgcagtgg ctatatccaa gaatttgaat gggtagaaga agtcacactt gccgttgcag
     2281 aacgagaaaa aataaaagaa cggtttatcc atgcgactta tctgatcgtt ttgcaaggtt
     2341 gggtagatgc agaagaaaaa cagaatttgc tgcatgtgtt aaacgaaacc gtatcagaag
     2401 aagatatata cgtcagtttc gaaacaccaa actcagaaga gattcagcag gaagtcccta
     2461 ctaaattgaa aaaccatccg cttgtcgaac cttttgaatt gctgactgaa atgtacagtc
     2521 tgccaaaata tgaagaagta gatccaacac cttggatgac accattttat cttgtcttct
     2581 tcgggatgat ggtcgcagat gttggttatg gtttgctgat gctcattggg acaatactag
     2641 ctcaaaaact acttgttcta ccacgcggga tgaaacgttt cgtaaaattc tttgagattc
     2701 tctcgatacc atcgatcatc tggggattga tctacagttc atttttcgga cagtcattac
     2761 caaaagaact gtttggcatc cgcttgcctt tcccaatcct atctacgacg gatgacgtca
     2821 atacgatttt gattttatcc gtgattttcg gtctgataca gattttggtt ggattatttg
     2881 tggcagcaaa ggaaaatatc aggagaaaag attacttgag tgctgtcagt gatggatttg
     2941 cctggcaagg gatcctaatt gggatcgtta ttgcgttggt tggtgccatg ttgataaaaa
     3001 atacagcatt tgtttacctt ggcgctgggc tatcgatcat tgctgcctta tgtatcttga
     3061 tcgttccgat tatccaaacg ccgtctaaag tcaaaggtgc tgccaaaggt gcgtataatc
     3121 tttacggact gaccggctat atcggtgatt tagtcagtta tacacgtttg atggcattgg
     3181 gtatttcagg cggaagtatt ggagcggcat tcaatatgct agttgctttc atgccgccag
     3241 ctgctcgatt cagtgtaggg atccttttga tcatcgcttt acacgcactg aatatgtttt
     3301 tgacactgct aagtgcctat gtccatggcg cacgattgca gtatgtcgaa ttttttggga
     3361 agttttatac aggaggcgga cgtgcctttg atccgctgaa aacggcggaa aaatacgtca
     3421 acatcaatca caagaaaaaa aacaataaat aattggagga tttttatata atgatggatt
     3481 acttaattag tcaaaatggt ggaatggtat ttgcggtatt agcgatggca acagcgacga
     3541 ttttttcagg gatcggttca gctaaaggtg ttggaatgac aggtgaggca gcagcagcat
     3601 tgacaacaag tcaacccgaa aaattcgggc aggctctgat tctgcaattg cttccaggca
     3661 cgcaaggttt gtatggattc gttattgcct ttttgatttt tatcaactta ggaaatgaca
     3721 tgtcagttgt acaaggactg aactttctag gtgcttctct gccgattgct tttacaggct
     3781 tgttttcagg tattgcccaa ggaaaagtcg cagcggcagg tattcaaatt ttagcgaaaa
     3841 aacctgaaca tgctacaaaa ggaatcattt ttgctgcgat ggttgaaaca tacgcgattt
     3901 taggtttcgt tatttccttc ctattagtgt tgaatgccta agcataaagg aggaaaagca
     3961 gatgaacgcc attgaaaaaa tcatctctca gatgaacgaa gcagcagagc aggaaagagc
     4021 ggcgttagaa caagaagagc gaatgaagat cgatcaaaac tttgaacaaa aacgtacgca
     4081 agttgaaaca gaacatcaaa aacaaaaaga aaaacaaatc gaattactcg aaaaaaaata
     4141 ccgtcagcta aggaatcgcc agcaagtaga agtacgacaa gaaaatttga atgcgaagca
     4201 ggaattttta agacgattgt ttgcagatgc ggtaactgaa atggaaaatt gggatgaatc
     4261 tgaacaaatc cagtttatca aaaatgcatt gtattcatta cctttgacag gaaaagtagc
     4321 attcattgct ggagaaaaat cagcagccta tctttctcag acgttgttgg atgaatggaa
     4381 caatgaactt ccctttatga tggttttaag tgatgaaact gttgctgatc aagcgggttt
     4441 tctgataaac gatcaaggag ttcagtataa ttttcttttc agcagccttg tacaagatat
     4501 ccaaggaacg atgagttttg agattgccaa tcaattattc gaataaggag ggatcagtgt
     4561 atggaatacc acgaactaaa tcccttgatt cgcggaagag agcttgaact tttgtccaat
     4621 gagacatttg aacggatgat tcaaacaaat tctgtcgaag cacttggtga aatattgaaa
     4681 tccacgattt acagtccata tatttatgac ggatatgaat atgacttcga acagaatctt
     4741 ttaaaggagc agagccgtct atttcgctgg ttaaaggaat cagcaccgga gcctgaaatc
     4801 gtttggatct atacgatgcg ttatactttc cataatctga aggttttgac aaaagctgag
     4861 atgacaaatc aaaatctgga ccatttgtat atcgacgatg gattttattc attagaaacc
     4921 ttgaaggatg ccattcatac gcaagtttca aatgaacttc ctgaaagcat tatggacagt
     4981 atacgagaag tacgtgatta ttttgaagag tctaccattc ttcagggaat tgacgtgatt
     5041 tatgatcgac agtttttgac agagcagcga cggcttgccg ataagctagg atatcaagaa
     5101 ttgctgacag aagtcattgc ttttattgac ttgaccaata tcacaacgat ggctcgagga
     5161 atattgcaga aaagaacata tgggttcatg acgacggtac tttccagctc gggaagtatc
     5221 gaaaaagaag tatttttgaa ctttgtagat aaagatatgg aagattacac gaattttcta
     5281 ttagagacag attatgctga ttttctgcgc cctgtcatcc gtgagggaaa tattgatctc
     5341 gtaaagcttg aacagttgaa agatgattat ctaagttcat tgtatcaaga agcacaaacc
     5401 caggctttcg gaccactgcc actactagcc tttttgaacg caaaagaggt agaaagcaaa
     5461 aaccttcgtt tactggttat cggcaaaaga agccatttcg aatcagaaac aatcagaaag
     5521 cgggtgagac aagtctatga cgcataagat cggtgtagtt ggcgacaaag attctgtttt
     5581 gccattcaaa ttgtttggct ttgatgttcg ttatggtaca accaagcaaa ccgttcgtca
     5641 ggcaattgaa gaaatggcaa agaatgagta tggcgtgatt tatgtcacag agcaatgtgc
     5701 agcattgagc ccagaaacga tcgaacgtta caaggaacaa atgatacctg ctatcgtatt
     5761 gattcccaat catcaaggat cattagggat agggctggaa gaaattcaaa aaaacgtaga
     5821 aaaagcagta ggtcaaaata tcttatagta tgaaaaggag atagaaattt tgcaaattgg
     5881 aaaaatcata aaagtctccg gacctcttgt aatggctgag aacatgtctg atgccagtat
     5941 ccaagacatg tgtctagtgg gagatctagg agttatcgga gaaattatcg agatgcgtgg
     6001 cgatgtggcg tctattcaag tatatgaaga gacttctgga attggtccag gcgaaccggt
     6061 tcgctctact ggtgaagctt tatctgttga attaggacca gggatcattt cacaaatgtt
     6121 tgacggaatc caacggccat tagatacctt tatggaaatc acacaaagta atttcttagg
     6181 gcgcggagtt caattgcccg cattggacca tgagaaaaaa tggtggttcg aaccaacagt
     6241 agaagccgga gaaacggtga gtgctggaga tgttatcggg atcgtagaag aaacaaaagt
     6301 tataaaacat aaaatcatgg tgccaaatgg gataaaagga acgatcaaaa caatcgaaaa
     6361 aggttcattc actattgatg aaacagtatg tgtcattgaa accgaaaatg gtgacaaaaa
     6421 tttgacgatg cttcaaaaat ggccagtaag acgtagtcgg ccaatcaaag aaaaattaaa
     6481 cccggatgca cccatgatca ctggacagcg agtgattgat acattcttcc cagtgacaaa
     6541 aggaggagca gcagctgtac ctggtccttt tggtgcaggg aaaacagtcg ttcagcatca
     6601 aattgcaaaa tgggccgatg tggacatggt tgtctatgtg ggatgtggcg agcgtggaaa
     6661 tgagatgacg gatgttttaa atgaattccc tgaattgatt gaccctaata caggagaatc
     6721 gttgatggaa cgtacggtat tgattgctaa tacatcaaac atgccagtag cagctcgaga
     6781 agcttctatc tatacgggaa tcacaattgc ggaatatttc cgtgacatgg gttatgacgt
     6841 tgctatcatg gcagactcca cttcacgatg ggcagaagca cttcgtgaga tgagcggacg
     6901 tttggaagaa atgcctggtg atgaaggtta tccagcttat cttggttcac ggcttgcgga
     6961 atactatgaa cgatctggaa gagtcattgc attaggatca gaaggtcgcg aaggaagtat
     7021 aaccgcaatc agtgctgtat ccccgtctgg aggggatatt tcggaaccag tcacacaaaa
     7081 tacattgcgg gtcgtaaaag tattctgggg tcttgattcc agtttagccc aaaaaagaca
     7141 ttttccttcg atcgattgga tacaaagcta ttctctttat tcaacagaag tagggaagta
     7201 tatggatcat atccttcaag ctgactgggc atccatggtc accgagggca tgcgtatttt
     7261 gcaggaagaa cagcagctga atgaaatcgt gcgtttggta gggattgatt ccctttcaga
     7321 taatgaccgt ctgacattag aagtggcaaa atccattcgt gaagattatt tgcagcaaaa
     7381 tgcttttgat gatgtcgata cattcacttc aagagagaaa caattcaaaa tgttgaagat
     7441 gatcttgacc tttggtgaag aaggacgccg tgcgttgtcg ttaggtgctt atttaagtga
     7501 gatcatggac ggtaccgtat cgattcgtga aaggatcgga cgaagcaaat atatcccgga
     7561 aacagaatta gaaaaactag atcaaattat tggtgaaatc aagcaaacga tgcaaacgat
     7621 cgtttcagaa ggaggcatga gcaatgatta aagaatatcg tacgatcggt gaagtcgtcg
     7681 gtccattgat ggcggttgaa aaagttgctg gtgtcaaata tgaagaatta atcgaagtcc
     7741 gtatgcaaaa tggcgagatt cgtcgtggac aagtattgga agtccaagaa gataaagcaa
     7801 tggtccaaat attcgaagga acaagtggta tcaatttacg tgactcttct gtacgatttt
     7861 taggccatcc tttagaacta ggtgtatcgg aagacatgat cggtcgagta ttcgatggtt
     7921 tgggacgacc gaaagataac ggaccagaga tcctgcctga aaagcatttg gatattaacg
     7981 gagaagtaat caatccagta gctcgggatt atccagatga gtttatccaa acaggtatct
     8041 ctgcaatcga tcatttgaat acattagttc gtgggcaaaa attaccagta ttttctggct
     8101 ctggtcttcc tcataaagaa ttagctgctc agatcgcgcg acaagcaacg gtactcgatt
     8161 catcagatga tttcgccgta gtctttgctg ctattggaat cacttttgaa gaagctgaat
     8221 ttttcatgga agattttcgt caaacgggcg caatcgaccg ttctgtcatg tttatgaatt
     8281 tagcaaacga ccctgcaatc gaacgtatcg ctactcctcg tatggctttg actgctgcag
     8341 aatatcttgc ttatgaaaag ggaatgcatg ttttggttat catgacagac atgaccaact
     8401 atgcagaggc attgcgagag atttctgcag cacggcgaga agttccagga agaagaggat
     8461 atcctgggta tctttacacg aacttagcta ctttattcga acgagcagga agaatccgcg
     8521 gactaaaagg atcggttact caaattccga ttctaacaat gccagaagac gataaaactc
     8581 atccaatccc tgacttgact ggttatatta cagaaggaca gatcatcttg actagagagc
     8641 tttacaagag tgggattcag ccaccaattg atgtactccc gtctctttcg cggttaaaag
     8701 ataagggaac tggacccgga aagacaagag aagaccatgc agctacgatg aatcagcttt
     8761 ttgcagccta tgcacaagga aaacaggcaa aagaattagc cgtagtttta ggtgaatctg
     8821 ctttatctga tgtagacaag atctatgcga agtttgctga gcggtttgaa aaagaatatg
     8881 tcaatcaagg attctacacg aaccgttcga ttactgaaac acttgatcta gggtgggaac
     8941 tgctatcgat gttgccaaga accgaattaa aacgaatcaa agacgacatg ctggacaaat
     9001 atctgccaga agggaagtga gttctgatgg cacgattgaa tgtgaatccg acccgcatgg
     9061 aattgactcg attgaaaaaa cagttgacaa ctgccacaag agggcataaa ttattaaaag
     9121 ataagcaaga tgaactgatg cgtcaattca tcctgttgat tcgaaagaac aaccagctgc
     9181 gtcaagaaat ggaagagtct atgacagaag caatgtctga ttttgtattg gcaaatgctt
     9241 cgatcacgga acctttcatc gaagaattat ttgttttgcc ggcggaaaac gttgatttgt
     9301 ctatcgtaga caaaaatatc atgagtgtaa aagtacctgt gatgaatttt gaatatgatg
     9361 aacagctgac agacacacct ttggaatacg ggtatttgaa ttccagtgcc gaactggatc
     9421 ggtcaatcga tcggtttact accctgcttc caaaactttt ggaactgaca gaaatcgaaa
     9481 aaacttgtca actgatggcg agtgaaatcg aaaagacacg tcggcgagtc aatgcattag
     9541 aatatatgac gatccctcaa ttagaagaaa cgatctatta tattcgtatg aagctggaag
     9601 aaaacgagcg cgcagaagta acaaggttga tcaaagtaaa aaatatgggt accactgact
     9661 aagtggtatc tttttttaaa agaggaaaac gatgattaaa caaagcaaac aaataaagaa
     9721 acgtttgccg gctgttcagc ttctcgcatt aggatttttt ctcctgattt tagttggcgg
     9781 cggcctactt accctaccat tttttagtaa tagtgggcat gggacaaatt ttatcgatgc
     9841 gcttttcact gcaacttctg ctgtctgtgt aacgggattg acaacattga atacagcaga
     9901 gcattggaac agtgctggtc aatttttgat catgcttttg attgagatcg gcggtcttgg
     9961 ttttatgatg gttccgattc tatgctttgc attgataaag aagaaagtca gtctcagcac
    10021 acgtattata ttaaaagaag cgctgaatct agaagaagta tcaggtgtga tgaagctcat
    10081 gctttacatc ctaaaattgg cctttgtgat ccaattgatt ggtacggttg ctttatcttt
    10141 tgtatttgtg cctgaatttg gctggttgaa aggtatctgg tacagtttgt tccatgcaat
    10201 ctctagtttt tgtaatgcag ggtttgattt gttaggagac agtttagcca atcaccagca
    10261 aaatgtttat ttgatcatgg tggtatctgc tttgatcatt tctggtgggt taggcttcat
    10321 cgtatggcgt gatttactgg aatatcatca gaaaaagaag atgacgattc attcaaaaat
    10381 cgcattaacc gttacaggta cccttttatt aggcggattc gttgtgttct ttttcacaga
    10441 gcgcaatggg gcaacgctag tggaagggaa tatgtttcaa cgattggcaa acaccttttt
    10501 tatgagtgtg acacctagaa cggctggata ttattcagtt gattacctcc atatgagcca
    10561 tgcaggtctg attttaacga tgtttttgat gtacatcggc ggaacatctg ggtcgacggc
    10621 cggcggattg aaaacaacta cgttaggtgt gttgctgatc cagatgcgtt cgatgttgaa
    10681 aggacgtacg agagcggaag catttggccg taccatccga caaggcgcag tgttgcgggc
    10741 acttacttta ttttttgtta cgttgacgtt gtgtgtagtc gccatcatga ttttatcggt
    10801 gactgaaacg atacctaaaa cttctggcat cgaatatatt gcttttgagg tcttctctgc
    10861 gtttggtaca gtaggtctta cgatgggatt gacgccagac ttgactgcaa tagggaaatt
    10921 gatcattata gcgcttatgt acattggacg agttggtgta ttgactgtgg tcttctctct
    10981 gatgacaaaa gcacaaagaa cacaatcaaa atacaaatat ccagaagaaa gtgtcatgat
    11041 tggataattt catcttctga cagaaaccta gtgatttagt aaaataagaa aataaaataa
    11101 tttttcttgc ttcttggata ctttaagtgc aaaatgttcg tttatttttt gaggcactac
    11161 ccgttgttga acctcgggta gtgcctcttt tttattgaaa ggaatcaata agatttgatt
    11221 cctttattta tcaataggtt ataattcata tttttttatt ttttctttta cgatacttag
    11281 tccagaagct aaaatttcaa ggttattaga ggatagggaa attcgaacga atgcatctgg
    11341 ttgcgcacgt gtagtaaagc gtctggaatg ataaagtcgg ataccgttag caagaaagtc
    11401 attctcaatt atcttagggt caatctcagg atcaactgga agaattcgat agtaagggac
    11461 acgattcgtt ggtttttcta ctttgaaaag agaatcaaaa agctgattgg ctctttttgt
    11521 aagacttaat ttagtttcta agatcctatt tgcttcattt gaatggagga tttgagtgat
    11581 gacttccaca tctaatccag aagttttcac atttatatta aaaattgctt gttctatctt
    11641 gctttggaca tgtactggaa ctacaagata agcaactcgc aatccagaac aaataaattt
    11701 tgtcattcca gctaagtaga ttgtttggtt aggtaataaa ttttggaaag gaggaatgat
    11761 cgtatgttga tagtaagttg ttaaaaatga atggatatca tcttcaataa cccatagacc
    11821 ttctttttca atcacttcgg ctaattgctc ccgtcttttg tgagagattt gaaaccctat
    11881 aggattatta caagaaggca taagaaagac accatgaatc ttcttttttc tacattcctg
    11941 aatcaatata tcaggtcgca ttccttgctc gtcaaaatca attggaacga tctctaaatg
    12001 atataactga gccaattcaa tgaaattagc gtatgtatat cggtctgttg ctattctatc
    12061 acctggagag aaaagtgcag aaagtgtgat agccaatcca ttttgaacac cggaaacaat
    12121 agatagagat tctaaggacg gaatgactcc ttgagttttt aaccaatttg ctgcagtagt
    12181 caattgaaaa tttgtgcctt tgggatcttg gtaattaagc aaaccaacaa gatttgggtt
    12241 attactagtt gattgaataa acgggacaac gaatttatta cattcttcaa aagaactaac
    12301 cagaccaaaa tcaatgatat gttcggttac ttcattggta gagatggtga tggactcaaa
    12361 gacattggga gaaataaatg tgccacttcc aatattcgta tacagcaaac ctttctcaat
    12421 tccatattta taagccttgc caacggtagt aaaatttaaa tctaaataat cagctagctc
    12481 tctttgggag ggtaactgag tgtttttctg taatttattc tttaaaatat cttgttctaa
    12541 ttgttgaatc aacgatcgat aataaggacg cttcaatttt tgtttgtctg gtcgccaagt
    12601 taacgggtaa ttgtcataag aattaatagg cattttctcc actccttaaa attgtatacc
    12661 atacaatttt accattgatt gtattgattt tagaagttaa tcttttgtga ggagggaatt
    12721 gtctatgaaa attattaatt ttgaggaagt agtagcttct tttagttttg aagaagcgat
    12781 tcaagtaatg aaaaaatgtt tttttgatta tgaaagtggt aaaatcagcc aaaatcctcg
    12841 attggtggaa attttacctg acggaaaagg caaaaatatg tttgcgatga tgcctgctta
    12901 tttgggggag aacagatatt ttggcacaaa agtcattacc gcttttccgg ataatcatca
    12961 gtatcattta ccttctcatt taggagagat tttcttgttt gatgctcaaa acggactacc
    13021 caaagcattg ataaacgcaa atgcagtcac ctggattcga acagctgctg tatctgcgct
    13081 ggcaacagat tacttagcaa atccgcatgc agaaacactt gctttaattg gagctggtca
    13141 acaagcattg tcgcatttga ctgcgatttc taccattcgt cccatcacta cagtgtttgt
    13201 ttacgatatt ttagaagaaa gacgaaatac ttttatcgct aaagctaaaa aacagtatcc
    13261 taaaataacg tttataaatg caatatcagt agaagatgca gtacgtccag cggaaatcat
    13321 ttgtacgttg acctctagta aagaggcatt tcttcaaaaa gagtgggtaa gggaagacgc
    13381 acatatcaat gctgtgggca catttactcc tgatacaaga gagataagta catcgttgat
    13441 ggaaacttct caaatctatg tagatgatta ctcagctgcc ttaacagaaa gcggggacct
    13501 gcttatagcc atggcagaag gagatttaga ggaatcttgt attgttggta cattaggcga
    13561 attagtttct gagaaaaaaa cattttataa aggagaatcg aggccgacga ttttcgacgc
    13621 agttggtcta gctattgaag atctttgttg tgcagaatat atttataaca aattagagaa
    13681 aggggcttta aaatgaactt agattattac gtagttgatg catttgcaga tgaggtgttc
    13741 aaaggaaatc cagcagcggt ttatgtgcta gaagaatggc tgccagaagg tacgatgcaa
    13801 aaaattgcca ttgaaaacaa tttatctgaa acagcattta cagtgaagaa aaaccaagaa
    13861 tttgagctta gatggtttac gccagatcga gaaatcgatc tttgtggtca cgcgacatta
    13921 gcaacagcat ttgttttatt taactattac aaaatacctg atgaaacgat taaattttct
    13981 actcaaagcg gtaatctatt tgtgacaaga attaaagatt attattacat ggattttcct
    14041 agtattatgc cgaaaaaagt gccgatattg tctgaatatg aagaagcgat tggggctaag
    14101 attaaagaag cctacttagc acgtgattta tttttcgtat tagaagatga aaaaacggtg
    14161 gctaaattgc agccagattt tacagctatc aaaaattttg aattaggaat aggagtcatc
    14221 gtgacagcag aaagtgtgca aaaagacttt gtttctagaa ccttctttcc aaaattaaca
    14281 atcaatgaag atccagtatg tggatcagca cattctaatc ttattcctta ttgggcagaa
    14341 agaatgaata aaacaaaatt gtcagcttat caaatgtcat caaggggcgg ggatctagaa
    14401 tgtgaattaa aagaagatcg tgtgataata ggaggaaaag cagttttgtt ttctaaaggt
    14461 acctcgttta tttgatttct ttcaaatgaa ttaaaaataa attggtggag agtggcaacc
    14521 ttatagctac aatggttgtt aaaaaaagac gcaaactttt tcaatttaat gaaaagtttg
    14581 cgtctcattt taattattgg tgtttgtttt ttattcaatc aagagtaaaa tgccccatac
    14641 taactatcct cggatgactt cgattttata accatcaggg tcaatgacaa agtagtaaga
    14701 aggcgcagtg ccaggtaatc cttttaagtc agtaatctca agaccagcag ctttatgttg
    14761 ctcgtgtaat ttttctagat catcagctgc tatagcgata tgaccatagc cgtctcctag
    14821 atcatacgca ccatgatcat agttgtaagt caactctaat tcatagtcat ccccaggaag
    14881 tgtcaaataa acaagtgtaa atttgttttc tggaaaatca cggcggcggc tttcttcaaa
    14941 accaaatgcc ttttgataaa attcgattga tgcttcaaga tctttcactc gaacacatgt
    15001 gtgagccatt ttcatagatt catctctcct tttcctttta ctcaatcata acaaaaagaa
    15061 gtaaggaaat agttgatttg tgcttgttca tgagaaattt ctcatgtaaa atggatagaa
    15121 aataaaactg ttaaggtgga aactattcat ggataaacca atttttggtg aaaaaaagca
    15181 atttgctgat tatcaaaccc gttatgcagc atatattgtc atagaaagag atcagcagat
    15241 tgcagtaatc gaggctccaa atggtgcttt ttttcttcct ggcggggaaa ttgaaggaga
    15301 agagacgaaa gaagaagcga ttgtccgtga gctgatagaa gaaatgggaa ttgtggcaga
    15361 aatttcttct tatctgggag aagctgatga gtatttttac tcgaattatc gtgcaactta
    15421 ttattataat ccaggatatt tctttgttgc tgattcttgg aagcgtatag gagaaccaac
    15481 tgaaaagacc aataaaattt ggtgggtgag ccctcaagaa gcgcttgtga aattgaaacg
    15541 tggtagccac cgatgggcag tcgagagatg gatcgaaaaa aataaaaact gaaagaaagc
    15601 gagtagatca gctttctctc agttttttta tttattacag tacatatttt ttgattgctt
    15661 cagctacacc gtgctcgtcg tttgatgaag tgatgacatt tgctgcttct ttgacaagag
    15721 aaacggcatt tcccattgct actcctagac cagcatattc aatcatggat aagtcgttct
    15781 cgttatctcc gatagccatg atttcatctt gatcaatacc aaggtgctgt gcgagattcg
    15841 caactgccgc acctttactt gcttctttat ttagtacttc gaagtaatag ggcgcacttt
    15901 tgaccgttgt gtatttatca cggaaagttt gcggtaaacg cgcaattgct gcatctaaaa
    15961 tttctggttc gtcgatcatc atcattttga cgatgttcat gtctggtgtc atttcttcag
    16021 gtgttcgata ttttagcggc atcttgacta aacttgcttc gtggatcgta taagcgctga
    16081 tatctcgatt aggcgtgtag atcgctttgt catcgatcga atggagatga ctgccgacac
    16141 ggcgtgccat tacttcgatc tctaggaaat cttcatgtgt cagtccgtga cgggcgatga
    16201 ttttacctga tttcgtttgt tgtacgagtg ccccgttata agtaataacg taatcattat
    16261 cttgaaaaag gttcaactcg tttaactgtt cctggacacc tggtaaggga cggccagtgc
    16321 ataaaacgat cgaaacacct tttgcaacag cagcggcaat cgcttctttt acttcttttg
    16381 ttacttctct tttctcattt aataaagtac catcgatgtc gatagcgact agtttgatag
    16441 acataatttc ctccaatata ttttaattta tttcaatcaa tgcgccatta cggatatggc
    16501 tggcaaattc ttgataagtt tcgtcaaata aatcataatg atcacgcaag gacggatcaa
    16561 ccatttcttt tggaaagaaa aatcgctcat ctccttgttc ttgtcccgct aatgcagcaa
    16621 caagctgact aacctgtgat aattctatca aagttccgtc atttcgcaaa atctcgattt
    16681 gtgtgcgatg tctgccttgt tctgggcgat aaaaatcata aggtagatcg tagctagaat
    16741 tcacagcagt gtaataaaca ggattatatc cgaccttttc tactaacaaa gtgagttctt
    16801 ggactaattt tgaatcccgg ttgccagaaa aagtagcaga tttcagtggt ttacgattca
    16861 aaaaacgttt agctaaatca tttaaaatag gatcagcaga ttcagaccat tgagtaaagt
    16921 aagtgcccaa gaccccatca tcaagtttga ggtattcttc taaagtaaag cttccgtcaa
    16981 gaaatggcag aagtaattga gaatggagtt caaaactgct ggcatcttct tgatacaact
    17041 cgtgtgcgcg atgaagcaga tgctccaaaa ttacttccat accacgggaa acaggatgga
    17101 agtacacttg cacatacatt tgatatcggc taacgatata atcttccact gcatgcatgc
    17161 cactcatgga aaaagcgatc ccgccttcat aaggacgaat cacacgtaat attcgggtaa
    17221 gatcaaatgt tccatattct gttccagtga aataagcatc ccgaagcaaa tagtccattc
    17281 gatctgcatc gatctgacta gaaatcattt ggacaacttg tgggttgggg taagtttttt
    17341 ggatcacact agctaccttt tcaggaaaac cttcttctac tcggttaaga atctgataca
    17401 cttctgtttc tggagaagtg atgatctcta cagtgatggc ttcatgattc gtatggaaga
    17461 tatgttcaaa tgtatgagag taaggaccat gaccgacatc atgcaacagt gccgcacata
    17521 aagtcaccag ccgttcctga tcatcccagc ctccgttgcc tattttttct tttgaaaaat
    17581 ttcgagaaaa gatatcgcag attcgtcttg aaatctcata aacgcctaat gaatgagtaa
    17641 aacggctatg ttctgctccg tgaaaagtga aagaagaagt gcctaattgt ttaatgcgtc
    17701 gtagacgttg gacttctttc gaattgatca aatctaagat tacttgatgc tggacatgga
    17761 tatagttatg gactggatca cgaaaaactt tttccatagg cagcatttga tatttataag
    17821 gggttgtcat aatgtagctc ctttatgtta ttacgttgtt ccattctctg gatttggttg
    17881 ttccattgct ccagctggat ttcttttttt agccagtcgg tagaatcctg taaatcactt
    17941 gcagaataca gcttttcaaa agcttttgca aatgcttctt tgacttcttt tacaaaaagg
    18001 ggttggtcaa gcagttcttc aagtgttgcc attgtagcag gatccactgc aggatagcca
    18061 tttgtaccaa agtcttcttt taaagcagat tgataaaatc gcttcatgac ttgcccacgt
    18121 ttttcctgat taccgttgac acttaagtac atcataacag aaatcccatt tttgatccga
    18181 cgctgagcga taccggcaaa ttttttgcca tggatgctta aatcataagt tccaggacaa
    18241 taagaatggg tgatttcgaa tgcttgtatc ggtgcctttg gaaaagcgta tttagtcaat
    18301 aggagcattt gctcgtaagc gtcatctatg gttatttttt ttgtgtcact ttgggggaaa
    18361 atcaaagaaa tattcaaaat tccttcatct gatacgatcc ctaatccacc tgaatttctg
    18421 atcactggag cgtaacctgc atcttccagt gtttggatcc cgtcacttag aaaaggagtt
    18481 cgaacatcct tcatacctaa gatgactgtg cagtcatatt gccagaaatg taaaaagatt
    18541 tgctggtgtt ctttagaata ttccgttaat atatctgtta atgcaaaagg ggaaaagtcg
    18601 tttttttgat aaatgtgctg atctagtcga atcgcagggt tcattcaacc ggctcctttg
    18661 tataaattca tattttctac aatattatac tctaaaatca aagagttgtt ttggaaaaaa
    18721 ggttttctgt aaaaatggac aagttggttg acaaaacaaa agaaactagg aacaatgtag
    18781 ctatgagaag taaaggagaa tgaataatgg aattatcaca aggaacaccg atttcaatca
    18841 aattgcgtac aaaagtaaaa cagcaagggg aagtgcaaga tttttatttt gatttgaaag
    18901 ggcaaatggt gacaattggc gatacgctgt acatacgtta taaagagatt caggaggaga
    18961 cgggcgatga gattcctgta acgatcaaat tagttccaga tggtcatgta cagctgatcc
    19021 gtgcaggaga aatgcggatg cgtttgaaat ttggctataa agaacggtta gaaacatcct
    19081 atcgaacacc atacggtatg atccagattg ctacttttac gaaagaactg catgttagtt
    19141 tgaaagaccg tcccacagca gggaaggttc gaatcgatta tgatctgttc atgggaccgg
    19201 aaaaaatcgg cgaatattat ttgaccttgg attttacagc gtaaatataa aagaaaagtt
    19261 tgattttttg tgtctgattt tgtatgataa ggagtagact gtgaaaggac gtgtcctgat
    19321 tggaaattag tgtatttgaa ggtgcaaaca aaagcgaatt atcaatgatc gaagtagcac
    19381 acgcgatctt agaacaacgt ggcgatgtca tggatttttc tgatttagct aaccagatcc
    19441 aaaactatct tgggaaatca gacagtgaaa ttcgtgattc actcgcacaa ttttatactg
    19501 acttgaatat tgacggcagc tttatctcat taggggataa tcgttggggc ttgcgttcat
    19561 ggtatgctat cgattcgatc gatgaagaag taaaccatgg aatggacgaa gaggatgaag
    19621 atactccacg tcgccgtaaa cgcaagaaag tcaacgcctt tatcaatgat gatgaagacg
    19681 cgatcgatta caatgacgat gacccagaag atacagatct gacagaagaa gatgacgatg
    19741 atctgtttga tgacgatgat gatgaagatg aagaaatcgc tgcttataat tctgacttgc
    19801 aagaaatcgg agcagatgac ccgacagatg atgaagaaga cttgccagga aatatcgaag
    19861 aagatttaag tatcatagac gaagacgatg atgatgaaga ttatgaagag gaagaatttt
    19921 ctgactctga aaaagaataa acattatttt cttacgagag gttgtgacag agacgatcag
    19981 cctatcagaa taaggaggtg tgacacgatt tttgtcacac cttctttttt gtatataaaa
    20041 atcccaagta aatacataaa aaataaaaaa attgaaactt ttagcgcacc ttgtacgtca
    20101 tattaagtga ggcagaaagg agtggagaat gatttttact ggatataaga aaaaacggca
    20161 gatcgaaaaa taccgtcacc tgctgaatgg actcatcgaa actgattatc aaaaaatgtt
    20221 ccgtatcacg atgaattacg tacatgataa agaagaggcc ttagatgtga tgcaagatag
    20281 ttttcataaa gccctgaact attttgaaaa agaaaaagag atcgagcatt ttagtgcatg
    20341 gttttatcgg atattgatcc gcacggcatt agatagttgg cgcagacaaa aaaggacgcc
    20401 tgtggactta tttgaaattc aagatatccc ccaacagtta cctttagaaa cttccgttgc
    20461 agagctccat atgattttag ataaaataga cagtccggaa aaagagattc tgatcctcta
    20521 cttttttgaa ggatttcgtt tgaaagaaat tgcgactatc ttggatatga atgaaaacac
    20581 agttaaaacg aaaatgtacc gctcattgaa tgctttacgt caaatcctag aatgaaggag
    20641 agaagagtgc catgtattcc caaaaagaaa atttaaaaaa actaaagaaa acttataaag
    20701 aacaagcaat tcccaaaggg cttaaagagg agatccgcca gcgttttatt atcgaagagc
    20761 aatcatttat aaaagcaaag cggagaaaga gacgtctcca agggttcgga gtatctttgg
    20821 ctatcgtaat gattacaaca agtagccttc tttttaatag tcaagttcga agctttgcag
    20881 aagatttacc catcttagga tcagtcattg aattgatctt aggcgaacgc tttactgatc
    20941 gatctgaaaa aatcgatatt caggtaccga agatcaatac acaaaatgag aaagaaaata
    21001 aaacaattca tggattaaat caaaaatact ttcgcgaagg acaagcagat tttgaaaaag
    21061 ctgaaaaaga atatggaaat tttgaaacgg atcattacca agttttggga gattatcaga
    21121 aaatcgtaga cgataaccgg tttctcgtga ttgaaaggca gatcacccaa acagcagcag
    21181 atagccatgt tgaaaaaaga tacgacacaa tcgacaagaa aaacagtgtc cagctctcac
    21241 ttccactttt attcaaagat gacacctatc tatctgtttt gacaaaagaa gtcaaacggc
    21301 agatggccga acaagtcaag gaagatccat ctaagtacta ttggactgag caagatattc
    21361 aagaagggac gatagaaaaa ccaacacttg ttacgcctac acgaagtttt tatttgaaca
    21421 aagagcatca attagttttg actttttctc aatacgaaat tgctcctggt tatatgggta
    21481 caccagaatt tgtcattcct aagtccgtga caaaaacgat tttggcatcg gaagattatt
    21541 tggatcatta gacgagaagt agttgcgact agcagtttca attttcaagt aagtacaagt
    21601 aaataataag aagagaaaaa gagaaagaag agatatagtg aaaagaaatt atttagttat
    21661 cagtattatt gctttatccg tgcttttgtt tttcgaaatt acttcggtgg cctccttgat
    21721 caccgaagta aaaggacaaa cacactcttc ggagcattcg tcacaatctt tcaaacaatc
    21781 agaacaatca accattaaaa gccaatcaac tgaaacagct aacgtcaaag aagccattca
    21841 agaatcagcg gaaaaggtac aaagtcagtc atcacaatca acacaagaca gtacaaaaac
    21901 gagccgagat ccggctgtca aaagcgtcgc aatcagcttt gatgatggac caggagcgac
    21961 cacaacaccg cagcttctgc gaatcttaaa agaaaaaaac gtgcatgcca cttttttcgt
    22021 cttaggagaa aatacggcgc agcatcctga aatcgtgaag cagacagctg aagcagggca
    22081 cgagattggc aatcacacat acgatcatca gaatctggcc actctttctg cccagtcgat
    22141 gacggaggaa gtgacgaaag cagacactga gatcaaaaaa gtcactggca aaacccccac
    22201 ttttgttcgt ccgccctatg gttctgtcac gaatgtagga gcaacgatca tccagcgacc
    22261 gatcatcgaa tggtctgttg attcagaaga ttggaagaca agaaatcctg atttgatttt
    22321 gcagaagatc caagcaacag tatacgatgg ggcaattatt ttattccatg atatctatcc
    22381 tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg aaagagcaag ggtatcgcat
    22441 cacgacagtt ggggatttat tagggcatcc aacggctgtc gagaactatt atggcagaaa
    22501 cgatcatcgg ccagttcagt aagtgagaca agtatcttta ataaataaca cattttaata
    22561 agtagttgaa cacataacta ccttacttgc aaaggcttaa caatatctat acaggtacaa
    22621 caaaaaactg tccaccaatt ttctttggcg gacagttttc acttaaaaat aaagaagcaa
    22681 ctattcgtaa attatgctaa aatagcagta gaacgcgtcc tgagggaatc gaacccccgt
    22741 ctcaagaacc ggaatcttac gtgatatcca ctacactaag gacgcaagca ctgggataat
    22801 tgtaactaaa cagataaaaa attacaaggg gattggacaa aaaaatgaaa tttgagcaaa
    22861 atttttcaca aaaacagcag cagacacaga aactagccat gacgcaacag ttgcaacagt
    22921 ccatccaagt tttacaatat aacagtgagg agctgttggc attcgttgag aatcaagcaa
    22981 tggaaaatcc attggtggaa gttgttgagc cggaatggca gccagattac ataaaagctt
    23041 cttcatcttc ttatgaagga gaagagacaa attacctaaa tcagattcct gatacaaaag
    23101 ggtctttatt tgattcattg atcgaacaag tccatttgaa ttaccgagat acttttttga
    23161 ggaaaatcgt tctttatctt gtagaataca tcgatttgaa cggatttttg acgattagtt
    23221 tagaagaagc cggaaaagag attggagcta cgcctatcca aatgctcgat gcgttgactc
    23281 tgattcagca attagaacca gctggagtag gtgcacgtag tttgcaagaa tgtctcatgc
    23341 ttcaaacaga acgagatgat tatgcgccgg aattagctta tatcgtgttg gaagaatgtt
    23401 ttgaggaact ggtggagcga aaatggaaag aaatcgcaca acgtttttct gttgatctac
    23461 atgctgttca gcagatattc gactatttac aaacgttgag tccaagccct ggccggattt
    23521 tcgaccgtag cagcgaattg tacattattc ctgatgtccg ggtattagtg gacgacgata
    23581 aaaatgtaca agttatctca aaccgaaaaa atcagccaaa tatacgcttt caagagagtt
    23641 acttcaaaca aatgtcacaa caagcagata aagaaactga aaattattta aaagaacgga
    23701 aacaggaatt cgaatggttg aagaaaacga tcctacaacg tggggataca attttgcggg
    23761 ttgctcaagt cattgtttcc cggcaaaaag ctttctttat cgacaaggaa cgtccaatca
    23821 agcctcttac gttaaaggaa gttgcaactg agatagatgt gcatgaatcc acggtcagtc
    23881 gtgcagtcaa cgggaaatat ttggaaacag acttcggtgt atttgaatta aaaaaatttt
    23941 tcactacacg aattcctacg aatagtacag aacagacaga agacctttca gcagatacag
    24001 cgaagaaaaa gctgcaggaa ttagtcgatc aagaagacaa aaacaagccg ttgtctgacc
    24061 agaaattggt tgaattgctg aaaaaagatg agatagcgat ctcaagacga acagtagcaa
    24121 aatatcgtga tttattagga atcccgagtt catcaaaaag aaaacgttat gataactaat
    24181 cagtcaaaag actagaaaaa gtatttcact ttttctagtc tttttagttg aaaaacgaga
    24241 gcaaaactgt tagacttaca gtcgtgaggt tgagatttcc ctttattgat gtgaaattcg
    24301 aatagttaat ttcttttttt atcgtatatg ggtcgtttta agtctacatt ggacgtaaag
    24361 tgtccaacta aggagatcgc tatgctggat gaaataaaaa tgattgaatc tgttgcacca
    24421 gatattatcg aagtgttgca agaacgctat aaaattttac gaaatatcta ttggatgcag
    24481 ccgattggac gtcgaagttt atcggaaagt atggggatca cagaacgagt attgcgaaca
    24541 gaaacagatc ttttgaaaaa tctgaatttg atcgacacct caaaaagcgg tatgacgctg
    24601 acaactaagg gcgaagaagt ttatcaaagc cttgaaaact tcatggatca gcttttaggt
    24661 acgcatcaaa ccgaacaaca gttggcagaa tattttggta accaacgctg tatcgttgtt
    24721 tcaggaaaca gcgaagagca gaccaaagtg gctgatgcat tcggcgaagc gctgagcgaa
    24781 gccctagacc gactacttcc agaaggcgag aatatcatcg ctgtgatggg aggaactact
    24841 atggctgtgg tagccgaaca attgtctaat ctggaaaata agaaacggca taacctgttt
    24901 gtccctgcaa gaggcgggat tggtgaagcg ataactgtac aggcaaattc tgtcagtgca
    24961 agaatggctg ctaaagcaaa cggtaaccac cgtgcacttt atgttccgga acaattaagt
    25021 ctggcaactt acaattcttt actcaatgag ccatctatcc aagaagtctt aaacttgatt
    25081 agtgaagcga attgtgttat tcatagtatt gggcgtgcat tacacatggc agcacgtcgt
    25141 aagatgactg aaaaagaatt agtcatgctg aaacaagcaa atgctgttgc ggaatctttt
    25201 ggctactttt tcaatgaaaa aggtgaagtc gtttataagg tccctagaat cggcatagaa
    25261 ttgcaagatt tggaaaaagt cccgatcatc atggcaatcg cgggcggaaa atcaaaagcc
    25321 aaagcaattc gagcttatat gaaaaatgca ccaaaacaaa cgtggctcat cactgatgaa
    25381 gctgctgcaa atgagatttt aaaaggggta accctttaaa ataaatattt ttttgatttt
    25441 cataaggagg aaatcttaaa tgacagttaa agtaggtatt aatggttttg gacgtattgg
    25501 acgtctagca ttccgtcgta tccaagatgt agatggaatc gaagtagtag caatcaacga
    25561 cttgacagat gctaaaatgt tggcacactt gttaaaatac gacacaactc aaggacgttt
    25621 caacggaaca gttgaagttc atgaaggttc attcaacgta aacggaaaag aagttaaagt
    25681 attagctaac cgcaacccag aagaattacc atggggcgaa ctaggcgtag atatcgttct
    25741 agaatgtact ggtttcttca cttctaaatc agcagctgaa aaacatttaa cagctggtgc
    25801 taaacgcgtt gttatctctg ctcctggcgg aaacgatgtt ccaacaatcg tttataacac
    25861 aaaccacgaa acattaactg gtaaagaaac agttatctca ggtgcttcat gtactactaa
    25921 ctgtttagct cctatggcta aaacattgaa cgacaaattt ggtgttgttg aaggattaat
    25981 gacaactatc cacgcttaca caggtgacca aatgactcta gacggacctc atcctaaagg
    26041 tgacttccgc cgtgcacgcg ctgctgctgc aaacatcgtt cctaactcaa caggtgctgc
    26101 taaagctatc ggtttagtaa ttcctgaatt gaacggtaaa ttagacggag ctgctcaacg
    26161 tgttcctgta ccaacaggtt cattaacaga attagtaaca gttcttgaaa aagaagtaac
    26221 tgttgacgaa atcaatgcag caatgaaaga agcttcaaac gaatcttacg gatacaacac
    26281 agacgaaatc gtttcttcag atatcgttgg tatgacttac ggttcattat ttgatgctac
    26341 acaaactaaa gtaatgacag ttggcgacaa acaattagtt aaaactgttg cttggtacga
    26401 caacgaaatg tcatacactg cacaattagt acgtacttta gaatacttcg ctaacttata
    26461 agatagacaa acgtacaagt gtaaattata gacatgtaca tttaaaagcg gggaagcaat
    26521 cgcgcttctt ccgctttttt tgataataag tttattcagg aggaaataaa atggctaaga
    26581 aaacagtgaa agatatcgat ttaaaagaca aaaaagttct tgtccgtgtt gacttcaacg
    26641 ttcctttgaa agacggcgtg atcactgacg acactcgtat caaagcagca ttgccaacaa
    26701 tcaactacgt tctagaacaa ggcggaaaag caatcctttt ctctcacctt ggacgtgtaa
    26761 aaacagaaga agataaagaa ggaaaatctt tagctccagt tgcaaaacgt ctaggcgaat
    26821 tattaggtaa agaagtaaca ttcgtacctg aaactcgcgg agaacaatta gaagaagcaa
    26881 tccgcaacat gaatgacggc gacgttgtcg tattcgaaaa tacacgattt gaagatattg
    26941 acggtaaaaa agaaagcaaa aatgatacag aactaggtaa atactgggct tcattaggcg
    27001 atgtatttgt taacgatgct ttcggtacag ctcaccgtgc acatgcttct aacgtaggta
    27061 tcgcttcaac tggtatccca acagttgctg gattcttgat ggaaaaagaa atcaagttca
    27121 tcggtgaagc agtagaagaa ccaaaacgac caatgattgc aatccttggt ggcgcaaaag
    27181 tttctgataa aatcggcgta atcgaaaact tgattcctaa agcagataaa atcttgatcg
    27241 gtggcggaat gacttacaca ttctacgaag caaaaggaat caagatcggt aactctctag
    27301 tagaagctga taaagtggaa ctagcaaaag aattaatcga aaaagcaggc gacaaacttg
    27361 tcttgccaat cgacaatgtc tgtgcaccag aattttcaaa tgatgttgaa acacaagtaa
    27421 tcgaaggaga cattcctgat ggtttgatgg ctttagacat cggcccagca tcagtaaaat
    27481 tatttgctga tacattacaa ggtgcaaaaa cagttgtttg gaacggacct atgggtgtat
    27541 ttgaaatgag taactttgct aaaggtacaa tcggtgtttg tgaagcaatc gctaacttag
    27601 aaggcgcaac aacaatcatc ggtggcggag actcagccgc agcagcagaa caattaggat
    27661 ttgctgacaa attcacacac atctcaacag gtggcggcgc aagcttggaa ttgcttgaag
    27721 gtaaagaatt accaggacta gcagcaatca acgataaata aacgaccaag tttgctcaat
    27781 tctgacgagt aatcgatcaa aaggtgaagg gagttttcat gactccctag ccatttttca
    27841 ataaattaag gagtgtgttc ctcaatgcgt aaacctatta tcgcaggtaa ctggaaaatg
    27901 aacaaaactg catcagaagc aaaagaattt gctgaagcag taaaaaacaa aatcccttca
    27961 aatgacaaag ttgattcagt catcggatct cctgcattat tcttacaaga attaatgaca
    28021 tcagctgaag gtacagaact aaaaatctca gcacaaaact gctactggga aaactcaggt
    28081 gcgttcactg gtgaaacttc accagcagct cttgctgatc taggtgttca ttatgtgatc
    28141 atcggacatt cagaacgtcg tgaatatttc catgaaacag acgaagacat caataaaaaa
    28201 gcgaaagcta tctttgcaaa tggtatgaca ccaatcctat gctgtggcga atctttagaa
    28261 acctatgaag caggcaaaac tgctgaatgg atcgaaggac aaattacagc tggtttagct
    28321 gatctttcag ctgaacaagt gtcaaacatg gttattgctt atgaaccaat ctgggcaatc
    28381 ggtactggta aatcagctga cgcacaaatc gctgacgaaa tttgtggagt tgtacgtcaa
    28441 acagttgaaa aactttatgg aaaagaagta tcagaagcag ttcgtatcca atacggcggt
    28501 tctgtaaaac cagaaaatat tgctgaatac atggcaaaag aaaatgttga cggtgcttta
    28561 gtcggtggcg caagcttgca agcagattca ttcttgtcat tgttagacgc tgtaaaataa
    28621 ttgttgaaaa tttgcgaaaa atgcaagaaa tgtttgcact aatgagcaaa attaactaaa
    28681 atcgtatatg agggaaatcc cttaggaaaa aacaaactca aaggagagac aaaacatgtc
    28741 aattattact gatgtttacg ctcgcgagat cttagactca cgtggtaacc caacaatcga
    28801 agtagaagta tacacagaaa gcggagcttt tggccgcgga atggttcctt caggtgcttc
    28861 aactggtgaa tacgaagcag ttgaattacg tgacggagac aaagctcgtt atggcggtaa
    28921 aggtgtaact aaagctgtcg acaacgtaaa caacatcatt gctgaagcaa tcattggcta
    28981 cgatgttcgt gaccaaatgg ctatcgacaa agctatgatc gctttagacg gaactcctaa
    29041 caaaggtaaa ttaggtgcga acgctatttt aggtgtttct atcgctgttg cacgtgcagc
    29101 tgctgactac ctagaagtac ctttatacca ctacctaggc ggattcaaca caaaagtatt
    29161 gccaacacca atgatgaaca tcatcaacgg cggatcacat gctgataact caatcgactt
    29221 ccaagaattc atgatcatgc ctgttggcgc tccaacattc aaagaagctc tacgtatggg
    29281 tgctgaagta ttccacgcat tagcttcaat cttgaaagct cgcggattag caacttctgt
    29341 tggtgacgaa ggtggatttg ctccaaacct tggttcaaac gaagaaggtt ttgaagtaat
    29401 catcgaagca atcgaaaaag ctggctatgt acctggtaaa gacgttgttc ttgctatgga
    29461 tgctgcttct tcagaattct acgacaaaga aaaaggtgtt tacgtactag ctgattcagg
    29521 cgaaggcgaa aaaacaactg acgaaatgat caaattctac gaagaattag tttctaaata
    29581 tccaatcatc tcaatcgaag acggattaga cgaaaacgac tgggacggat tcaagaaatt
    29641 aactgacgta ttaggcgaca aagttcaatt agttggtgac gacttgttcg taacaaacac
    29701 tcaaaaatta tctgaaggta tcgaaaaagg aattgctaac tcaatcctta tcaaagtgaa
    29761 ccaaatcggt acattaacag aaactttcga agctatcgaa atggctaaag aagctggcta
    29821 cacagcagtt gtatctcacc gttctggtga aacagaagat tcaacaatct ctgatatcgc
    29881 tgttgcaaca aacgctggcc aaatcaaaac tggttctcta tcacgtactg accgtattgc
    29941 taaatacaac caattactac gtatcgaaga ccaacttggt gaagttgcag aatacaaagg
    30001 tttgaaatct ttctacaact taaaaaacaa ataattcttt tctaatgaag atacatgatc
    30061 gactattgaa agatagtcga gtatgatcac ttagttaagt aatcgaactc tctacttgct
    30121 gaaatatcag caggtagaga gttttttttg caactagctt ctgtatatga tgtttacaaa
    30181 gaatggtaca agaaaagatc atccttgaaa aaggaaagct aacaataact agcatcatat
    30241 aagagctttt aaggatagaa cagtagaata ggaatctatg agcaagtcct ctcgtataaa
    30301 taaaatagga gggaagtagc agcatatttt tcataaagtg gctctctttc ttcattttta
    30361 ctttggttgc tcctatttga aaattcgagc acactcaaat acaataaaaa gaaatggcaa
    30421 gggagaggat tagatgaaaa aagaagaaaa aatcgcgatt ttacaagaga tcatccgaat
    30481 taaatcagtg aatggaaacg aaggtgaagt agcagcctat ttgaataagc ttttggcaaa
    30541 gcatgacata acaggagaga ttgtttctta cagagatggt agagataatt tgattgctcg
    30601 ttaccaaaag ggccaatcag gaaaagtgtt aggcctatct gggcatatgg atgtcgtggc
    30661 ggctggtgat gaatcgtctt ggacctatgc accctttgca gcagaaatcc atgggaaccg
    30721 tttgtacgga cgtggtgcta cagatatgaa gagtggcttg gcagcaatgg tgattgccat
    30781 gatcgaactg aaagagtcag gaaaaccatt taacggaaca gtcaaattat tagccactgt
    30841 cggagaagaa gtgggtgaac taggtgggga acagctcacg aaagcaggct atgtagatga
    30901 cttagatgcc ttgatcatcg gagagccaac taattacagt ttgatgtata cgcacatggg
    30961 atcaatcaac tatacagtca cctcgcacgg aaaagaagcg catagctcta tgccagacca
    31021 aggttataat gcaatcaatc acctaaatga attcattacg aaagcaaatg ctgaaatgaa
    31081 tcacctcgct gaaacaatcg agaatcctgt cctaggaaaa acaatccata atgttacatt
    31141 gatcagtggc gggaatcaag tcaacagcat cccaagccat gcgcagctcc aagggaacat
    31201 tcgatcgatt ccagaatacc caaatgataa aatcattgca ttgctgcaat caatcgtcaa
    31261 tgaattgaat caagagacag actatcattt ggaattgatg atcgattaca acaaaatacc
    31321 tgtcaaagct gatccagatt ctcctttgat tcattgcatt cagcaacaat tcagccagcc
    31381 attaccttta gttggagcgg cagcaacaac ggatgcagcc gaatttacga aagctaacca
    31441 ttccttcgac tttgtcgtct ttgggcctgg tgtcgttact ttgcctcatc aagtagatga
    31501 gtatgttgaa atcgataatt atttggatat gattgaaaaa tatcaaggaa ttattttatc
    31561 ctacttagcg taagattttt ttaataaatg aaaaaacaga gccagattag cattattttg
    31621 ctaatccagc tctgtttatt cagcctattt ttctttttga atatatcaag cgttgtggca
    31681 acatagtgat gactaatgaa acaacgacga tagacaacaa acgatcaagg tagtctgtac
    31741 ctgcctgtac caagatcaca cttgttgtct gacttaaacc taacccatgt aatagctgaa
    31801 cgatcatact ggatcctgag gaggtgatcc cgccaaataa ctgaacggta atgatcgaag
    31861 aaacgatcgt tccaggtacc gacaaaatca aagcgagtaa taacagtttc aagctgtttt
    31921 tatctttgcc gagatatctt ggcaaaatca atccagcaag caagcctgtg agaatttgga
    31981 ttggcgaata atacagagaa aaaatatctg tagtcactcc acttaaaata ccgctggaca
    32041 atcctgtcag cattccaggt acaggcccaa gcaatgcacc agcaaaaatc gttccgatag
    32101 aatcgagata gattggcaaa cgaagccata aagcaatcgt tcctccaata taattgatcc
    32161 caatacacaa agccatcact gtaagtacgc gagttgccat tttgttttta ttcattttgt
    32221 tagcccatct ttaacctttc caaatctttc ataatcgttt cttcttgtgc atttaaaaga
    32281 acggttaaaa actttcggaa aaaaagacga gtatctactt ttgtcataat tttactgtta
    32341 gcaggtttct gccaaaattc ataacgatca accaatgttt gtccacgact tattccttga
    32401 gtttccacag cagtgaagct tttgaaacct tcacagatgg ccccctcgat aaaataagca
    32461 acagctaacg gatcattgat gacacatcct aaaattcttt cttgtttcca gtggaagtca
    32521 aaataaaaac gagtgatcgc ttttagatac tctccctctt caggattcat ttgacagcag
    32581 tattcgagga tagtcggtgt caagacgatt tctcttgtca catctaaccc aaccatttct
    32641 atcgtttgtt tgagattctc gaatacataa gaagctgctt caggatcaca ccagtaattg
    32701 tattcagcaa ctggcgaaca gttaccatgg cttttgtaag ttccacccat cgagacaaaa
    32761 cgatccatat gtttcccaat atttgggttg actttcaaag caaaagcgat attggtcaaa
    32821 ggacccaaag caatgactga tgtatttgtc ttttctttga aataatcagc caaaaaatca
    32881 acggcatgga gaggttctgc ctgttttctc aagatcatcg ggaaatttgt ttcccctaaa
    32941 ccatccatac catgagtgtc ttgtgcactg atgaaaggtt gcttcaaagg tttttccatt
    33001 ccttgataaa caggaatgtc taaacgatca cagcgttcca ggcatttcag cacattctgc
    33061 gtacctatat ctgcagggac attcccacaa actacggtaa tagcaactac ttcgatatca
    33121 ggggatttga cagctaatga taaagctaaa gtgtcgtcga tccctggatc gcaatcaata
    33181 attacttttc gcattatttc caatctcctt tctttagaga tacttagttt tttattctgg
    33241 gaagtttccg aaccagtcca agacgagcgt cttgattata atgcacgaaa ttagtatact
    33301 ggcttttatt gcgaatgtaa atgctctctt acaagtaaat ctattgagga gaactttaag
    33361 taaatgatac aatgaaaaag aaatggaatt tacgtgtgcc gaaaataaag aatcctatgg
    33421 atcggtacat tggataatag ttagattata gtttggcaga aagcggcggg tgtacaatgg
    33481 ttatttttta tttcattctt attctgataa ttatagggat cagttaccat tttctttctt
    33541 atttttatgc aaagaaatta gtaagagcgg gagtacagag aggatatgac tggtatgaag
    33601 acactggtca tcgtttgatg gaccctgctg cagtcacgtc agtagaaaaa aaacgacaaa
    33661 aattagaaga agagtgtgaa agcttttggg agcagggaac acccaaaaca atcaaaagtt
    33721 atgacgggtt gaaactagct ggtcagatgt tcatccatcc aaataagcag aataaatggg
    33781 tcatttgcgt acatgattac cgaagcacag ggaaaagaga catgtctcat ataggtaaaa
    33841 gatatgcaga gaaaggattc aacgttcttg tacctgattt acgtgcgcac ggagaaagtg
    33901 aaggagaaat catcggaatg ggatggctag atcggctaga tttaattgcc tggatccaac
    33961 tcatcttaga tgaacagccg aatgcgtcca ttattttaca cggcggatca atgggagctt
    34021 ctacgatcat gatggcaagt ggtgaaaaac ttccatcagc ggtcaaagga tttattttag
    34081 acagcggata tgtttctgtt tatgcagaat tccgatatat gttaagtaaa atcactgtct
    34141 ttcctaaaaa aatggtcatg agatatgcga atcattatgc ccagaaatac gccggttact
    34201 ccttaaagca agcatcagct acgagacagc ttggcagcaa ccatctgcct ttattggtca
    34261 ttcatggtga aagagatcat tttgtcccta cagaagcagc gtacacgatt caaaatgcta
    34321 cggcagggga taaagcactg ctgcttgttc cagaagcaga acacttggaa gcatcgatga
    34381 aagatccgaa aacctattgg acagtgattt tttcatttat tgaacaaaga ataacgatga
    34441 attaattttc tcttcttttt tgtaagtaat tcgaggataa ataattgttt atagatcagg
    34501 agactgctaa aataaggtag gaataaaaaa atatcagtta tttgaggagt gcggcatatg
    34561 gaaatcaaag aagaaaaaaa tcgtcttgct ttattcaatg atgaacaaca agagatcgga
    34621 gaaatgacat ggtcagatgc gggcccagac atcatgatca ttgaccatac atttgtagat
    34681 ccagcatatc gtggtcaaaa gttagctgaa aagctagtgt atactggagt agaattagca
    34741 agacgagacg ggaaaaagat cattccttta tgcccttatg caaaaaaaga gtttgaaaag
    34801 aaaccagaat atcaagacgt cttacgtcag tcataagaat aacagctgaa agttttgaac
    34861 tttcagctgt tattccattt atatagagtt gtcacggact tttatctatg ataaagttaa
    34921 agagacgata tcttttggga aggaggctgc gatatgtata atttaatttt gggaatcgtg
    34981 atcgttttat cgattgttat ggtcattgcg attatgatgc agccaagcaa gcaaaatagt
    35041 gcagcgagtg catttaccgg cggtgcggat caactattcg gcaaacaaaa agctcgcggc
    35101 tttgaagctg tcatgcagcg atcaactgca gttatgggag ctatctggat gatcttatta
    35161 tttgtcctag catatttatc ttcgaagtag ttgaataagc ctctcgcaaa acggcgggcg
    35221 gcttttttta ttgcaaagag aacgcttaag aagaagttat gaaagaaact atggtaaaat
    35281 gaggcgaaag aggtgagacg agtgagacaa ttaccaaaac ccctttatgc aaaacatgga
    35341 aagcgagcgg ttttgctgct tcacgcttac tcaggaagtc caaacgatgt tcgaatgctc
    35401 gcacgatttt tggaaaaagc agattataca gtttatgcgc cgctttttaa aggacacgga
    35461 acaatggagc catatgatat tttgcaagaa aaagcagaaa gctggtgggc agacactaaa
    35521 aaagccatcc attttttaca gtctgagcag ttttctgata ttgccgtatt aggattatca
    35581 atgggcggca tctttgctgt acgagcacta gaagaagaat cagtgattgg cggcggtttt
    35641 ttctgttcgc ctttgtctcc tgtaaaaaca aacgtgcctg aaaactttga aaaatacgta
    35701 cgtcaagtgc tgaagacagc aggtaaatca gagaaagaaa tcaatgaaaa agcagttgct
    35761 taccgctcgt tagctgaaca acaattaatg gatatacaag atcaggcagc aatcgtagaa
    35821 agtaggttgt cagatatcca acagccgata tttttagctc aagctgggaa agatgaaatg
    35881 atcgacccta atggcgtatt tgaaactgca cgcaagttga gccgtcagcg agtgacactt
    35941 cgatggtatc ctgaaagtgg tcatgtgatt acagttggta cagcaagacg cgaattagaa
    36001 aaagacgtct tggaattttt agaaaaatta ccttggaatg aggaataaat atgacaaaag
    36061 aaacgataaa agagaaaatt ctctttttca tggaaagcag tcgcaaacgc agtttttcta
    36121 tggaagaaat cgctgaaggt ttagggttcc aaaaaagcga tgattttaaa ttactagtac
    36181 aaacgattgc tcaaatggaa agagaacaga gtatagtttt caataaaaaa ggaaaagtga
    36241 aacttccttt aaaaccaatc ttgatcgaag gaacatttcg agcaaatgaa cgaggatttg
    36301 gatttgtaac gattgatccc gaagaagatg acatctatat cccaaaagaa gcgactggtt
    36361 ttgcaatgga tggagacacc gtagcaatcg atatcatgaa gcgagcaaat acagatatgg
    36421 atcgcggagc agaggggaaa gtagtcgaga ttcgagaccg ggcgactact cagttagcag
    36481 gggaattcgt tgcttattca gaagaagaaa tacaagaaac ggatctttac ggcgttgtta
    36541 tcccgaaaga tagaaagctc aaccaattta aagtatatgc agcagcagag ggagttcgtc
    36601 cagttgacgg aagtattgtg ctgatcgaac tgacccatta ccctgaaaaa gggtatgcga
    36661 ctagtttaga agggataatc aagcaagtga ttggtcataa gaatgaccct ggcatggata
    36721 tcctctcaat tgtcgtggct aatggtatcc ctacgaaatt tcctgatgat gtattgtctg
    36781 aagcagatga agttccagat caaatcagcg aaaaggatat aactggacgt agagatttaa
    36841 gaaatcagct cattgttacg attgatggag aagatgcaaa agatctagat gatgcagtta
    36901 cggttaaaaa attagataac ggtaattatt ttcttggagt acacattgca gatgtttctt
    36961 actatgtgac acaaggcagt caattggaca gagaagcata tgaaagaggg acaagtgtat
    37021 atttgactga tcgagtgatt ccgatgatcc cgcaacgttt atcgaatggt atctgttcac
    37081 tgaatcccca tgttccaagg ctgaccatga gttgcgaaat ggagattgat ccaaatggac
    37141 aagtagtctc tcatgatatt ttcccaagtg tgatccaaac aacagaacgg atgacctaca
    37201 cagctgtcaa tcaaatctta gaagatcaag acgaagaaac gatgcaacgc tatgctcagc
    37261 tcgtacctat gttccaagaa atgggtgaat tgcatcgaat ccttgaaacg atgcgaatca
    37321 aacgtggcgc gatttctttt gaggatcgcg aagcaaaaat ccttgttgat gctgaaggac
    37381 acccaaatga tatccagcta agaagcagag gaattggtga acgcttgatc gaatctttca
    37441 tgcttgcggc caatgaaacg gtagctgaac attataataa attaaatctg ccgtttattt
    37501 accgaatcca tgaacagcca aaagaagaaa aaatgcagcg cttctttgat tttgcttcag
    37561 ctttgggcat tctggtccga ggaacgaaag gaacgatcac accaaaagat ttacagcatg
    37621 tgatcgaagg agtagaagac aaaccagaag ctgcagttat caatactatg ttgctaagaa
    37681 gcatgcaaca agcacgctat tcagaagaca attttgggca ttatggctta gcggcagatt
    37741 actacacaca ctttacatct cctattcgac gctatcccga tctaatcgtc catcgtctga
    37801 tccgttctta cagtgaagat aaaagtgaaa gcaatcaaag ttcgtgggct acggaacttc
    37861 ctgaaattgc agatcatagt tctaagatgg aacgtcgtgc agtagaaaca gaacgtgaag
    37921 tagattccat gaagaaagca gaattcatga tggacaaagt gggggaagaa ttcgatggga
    37981 tcatcagctc tgttgtgaaa ttcggcctgt ttatcgaact gccgaataca gtagagggat
    38041 tgatccatat caatgaattg aaacaggact acttccattt tgttgaaaat catttagctc
    38101 ttgtaggtga aagaactggt ctgactttta aaatcggaca gaaagtgcga gtaaaagtag
    38161 tcaaagctga tccagatgaa cgagcagtag attttgaact tgtttccgca gaagaggtta
    38221 cagcgttgga acggccaaaa acacgaaatg gaaaatcagg aaaacgcgaa ggaaaacaag
    38281 caggaaaacg cccagaaaga aatggcaaga agaacaaacg atatagcgat agtaagcaaa
    38341 cgttcggtaa gaaaaaaggg aagaagccat tttacaaagg tgttaaaaag aagaaaaaga
    38401 aaaaataaaa tgagttggag gaaacagcta tgccaaaagg cgagggaaaa ttaattgcac
    38461 aaaacaagaa agctcgccat gattattcga tcatcgacac gatggaagca gggatggtct
    38521 tgcaaggaac cgagatcaag tcgatacgaa acagccggat caatctaaaa gatggattta
    38581 ttcgcgtccg caacggagaa gctttcttgc ataatgttca tatcagtcct tatgaacaag
    38641 gaaatatttt taatcatgat ccgttgcgca cgagaaagtt attattacac aaaaaacaaa
    38701 tcatccggct tgaaaatgaa ttgaaaaata ctggaatcac tgttgttcct ttaaaagtct
    38761 atattcgtaa cggctatgcc aaggtattga ttggtctggc gaaagggaaa aaatcttatg
    38821 ataaacggga agatttgaaa cgaaaagata tcgatcgaca aattgatcga acattaaaaa
    38881 atttctctag ataatttcat tatccttttt cgcatattcg gtgcttttgc ttttacttaa
    38941 tgataaaatt tgttagcatt taatctgata aaacatgatt atcataggtt ttttcttaga
    39001 tcaggagcac cgtgtataaa gaaaaattcg cttcgttttt gactgaaata aaaaagtgtc
    39061 taattttcat gaaaatgttt tgaattttct gtttcatggt ggtatgatac cgtgtggtta
    39121 gaaaccgatt ggaaaaaacc agatacgcac atgaattgat tttatgtgaa tttcggggaa
    39181 aagaggtgaa atggattgga tgaacgctcg ctaacgttcc acattggacc aatttggttt
    39241 gatggtacag tttgtataat ggtgttactg acctgtttga ttgttttctt cttagcttac
    39301 ttttttacaa gaaatcttaa gttgaaacca acaggcaaac aaaatgcgat agaatgggtc
    39361 attgatttta gtcgaggaat cgtaacagat aatctgccta gaaaagaatt gagcaatttc
    39421 catttgttgg catttacatt gtttttattt gtttttgttg caaacaacat cggtttagta
    39481 acaaaaatcg ttttacctaa cgaaacaaca ctttggaaaa gcccgacagc agacccgttt
    39541 gttactttaa ctttggcatt tataatgatc ttacttactc acttatttgg ggtgaaaaag
    39601 ttaggcttca aaggatattt cgtaaattcg ttcttgaaac catatagttt tatgtttccg
    39661 atgaaactga ttgaagaatt tacaaactta ttaacacttg ctttacgtct ttatgggaat
    39721 atttatgcgg gggaagtttt gctaaccttg attgcaaaaa tggtaataag cttaggctgg
    39781 ttctcattgc cgttagcaat tccattagag atggtctgga tcgcattctc cttgtttatc
    39841 ggaagcatcc aagcatttgt cttcgttact ttatctatgg tttatatgag ccataagatt
    39901 gaagtggaag aataatattc gttttaaaaa caaactacta tttatctaat ttaggaggaa
    39961 aacaattatg aattatatcg cagcagcaat cgcaatcatg ggagcagcaa tcggagccgg
    40021 ttatggtaac ggtcaagtta tctctaaaac aatcgaatca atggctcgcc aacctgaaat
    40081 gtctggtcaa ttccgtacaa caatgtttat cggggtagct ctagtcgaag ctgttcctat
    40141 cttaggggta gttgtcgcct tgattctagt ttttggtgtt taattgaatc agtattaagg
    40201 cagaaacaat ttggtttgtt actgcttttt tacatgaaaa ctagaagtac ttcaaagaaa
    40261 ggaaggctcg cctatgctga atcaattggc aattgcagaa gttggcaatg cgatgttagg
    40321 aaatgtcatc gttgtcagcg gctcattttt gatactactg gctctcttga agcattttgc
    40381 ttggggacca atcagcgata ttttgaaaaa acgtgaagac aagatcgcca atgatttgga
    40441 ttctgcagaa caatctcgca tcaactcagc gaaaatggaa caagaacgcg aacaacaatt
    40501 gttagcctct cgttctgatg cagctgatat catcaaaaat gcgaaagaaa gtggagaatt
    40561 aagccgccaa aatattttga aggatgctca agaagaagca gctcgtctaa aaagtaaagc
    40621 ccaagctgat atcactgtag aacgtgattc agcgctgaac tctgtaaaag acgatgttgc
    40681 agaactatct cttcaaatcg cggaaaaaat cttgaacaaa gaattatctc cagaaatgca
    40741 cgaatcatta atcaatcaat atatcgaagg tctaggttct tcaaatgaaa ctagataaat
    40801 atacagtagg aagacgttac gggaaagctt tatttgaact ggcgatcgat tcaaataaag
    40861 cagaagaaat ttatcaagat ctcgtaagct tacgtcaaat ctatgaacaa gtacctggat
    40921 taggtaatat gttaagtgat gttcgtctag aacctcatga aaaacgagaa atcatggaca
    40981 agcttgttag tggctatgat ggaatcgtta aaaacttttt agaagttgtt tatgcgtata
    41041 accgaatgga tgatcttttg ttcatgatcg aagaatatga acgtcgatat gatgaacatc
    41101 aaggtttgct gttgggaagt gtcacaacag cagttccact ttcagacgaa caacgaatac
    41161 aattagaaaa gaatgttgca aaaacaatga actatcaatc agtcgaatta aaacaaatcg
    41221 ttgattcttc tattattggt ggcgcgattg ttgaagctaa ccatcgagta atcgacggaa
    41281 gcattcgcac gcaattagaa aatatgcgta atcaattaaa tagatagtac aaagaggtag
    41341 gtgaatcgaa tggccatcaa agcagaagaa attagtgcct tgattaaaga acaaattgaa
    41401 aattatcaaa acgtgctttc agtcgaagaa atcggtaccg taacttatgt aggtgacggg
    41461 atcgctcgtg cacatggttt agaaaacgcg atgagtggtg agttgcttga attttcaaat
    41521 ggctcatacg gaatggcgca aaacttagaa tcaaatgatg taggtatcat tatccttggc
    41581 gatttcgagt ccattcgtga aggagataaa gtaaaacgaa caggaaagat catggaagtt
    41641 ccagttggag aggccttgat tggacgggta gttaatccgc taggtcagcc aattgacgga
    41701 ctaggtgaaa tcgttactga caaagctcgt ccagtagaag caatggcacc aggcgttatg
    41761 caacgtaaat ctgttaacga accaatgcaa acaggtctaa aagcaatcga cgctcttgta
    41821 ccaatcggac gcggccaacg tgaattagtt atcggtgacc gtaaaacagg gaaaacttcc
    41881 attgcgatcg atacgatcat caaccaaaaa ggtcaagata tgatctgtat ctatgtagca
    41941 atcggacaaa aagattctac agttcgtaca caagttgaaa cattgaaaaa atatggcgca
    42001 atggattaca caatcgttgt gaatgccggt gcgtctcaac cagcgccgtt gctttatatc
    42061 gcaccatatg ctggtactgc aatgggtgaa gagttcatgt acaacgggaa acatgtcttg
    42121 atcatttttg atgatctttc aaaacaagct gttgcttatc gtgaactttc cttgctgctt
    42181 cgccgtccac caggtcgtga agcttatcca ggggatgttt tctacttaca ctcacgctta
    42241 ttggaacgtg cagcaaaatt aagtgatgat ttaggtggcg gttcaatgac tgccttacca
    42301 tttgttgaaa cacaagctgg agatatctct gcttatatcc caacaaacgt tatttcgatc
    42361 actgatggac aaatcttctt agaaagcgac ttgttctacg caggtactcg tccagcggtt
    42421 gatgccggac tatctgtatc tcgtgtaggg ggttctgcgc aaatcaaagc aatgaaaaaa
    42481 gttgctggga ctttacgtct agacttagca agttaccgtg aattagaagc atttacacaa
    42541 ttcggttctg acttggatgc agcaacacaa gcaaaactaa atcgtggacg ccgtacagta
    42601 gaaatcttga aacaaaaatt gcatgcaccg ctagctgttg aaaaacaagt agtgattttg
    42661 tatgctttga cacatggatt cttagatagt atccctgtag acagtatttt agacttcgaa
    42721 catgaattat tcgaatatct ggataccaat cattcagata tctttgaaac aatccgtaca
    42781 acaaaagatc ttccagaaga agaagcgttg aacagtgcga ttcaggaata taaagacatg
    42841 ttcttagcta caaaaggaaa taattcctct acagaagata aattgaaatc cattcaaaat
    42901 gcatagtgag gtgagatgaa tgggtgcttc attaaatgaa atcaagacac gtatcgcttc
    42961 aacgaaaaaa acgagccaaa tcacgcgggc aatgcagatg gtgtctgctt ctaagttgac
    43021 aaaatctgaa gcttcttcac agaagtttca ggtttatgca acaaaagtaa gagaaatcgt
    43081 tacgcatttg actgctactc agttaaatga tattgcctca gaaaatccta gaggggatat
    43141 caattacaac agcatgttgc tcagccgtcc tgttaagaaa acgggttata tcgtcatcac
    43201 tgcagatggc ggtttagtcg gtggatataa cagctcgata ttgaaacaaa cgatgtctat
    43261 tttagaagaa gaccaccaat cacccgatga ttacgtgatg atcgcaattg gcggaactgg
    43321 tgcagacttc tttaaagcac gcggtatcaa tctagcatat gagctgcgca atctatctga
    43381 tcagccaagc tttgatgaag tccgcaaaat cgtaaatatg gcaacaacta tgtaccaaaa
    43441 cgaagtattc gatgaattat acgtatgtta taaccatcat atcaattcgc taactagtca
    43501 gtttcgtgta gaaaaaatgc tgccaatctc agacttagat ccagaagaag caactacttt
    43561 cgaacaagaa tatatttttg aaccttcaaa agaagaaatc ttgtctcagt tattgcctca
    43621 gtatgctgaa agtttgatct acggtgcgat cgttgacgcc aaaacagcag aacatgctgc
    43681 tggtatgaca gcaatgaaaa cagcaactga taatgctgct aatatcattg atgagctgac
    43741 ggtttcttac aaccgtgcaa gacaaggggc tattacccaa gaaattaccg aaattgttgc
    43801 cggagcttcg gcactagaat agtattgaga ttggaggaaa aaagatgagt tcaggcaaga
    43861 ttgttcaagt aatcggtccc gttgtcgacg tggaattttc tttagaccaa tccttaccag
    43921 atatcaacaa cgcattagtt gtttataaaa atgacgaaaa taaatcaaaa gttgttcttg
    43981 aagcagcgtt agaattaggg gacggagtga tccggaccat tgcgatggaa tcaacagatg
    44041 gtttacaaag aggaatggaa gtcattgata caggcaaagc aatctctgtt cctgtaggta
    44101 aagaaacatt aggtcgcgta ttcaacgtac taggagatac gatcgattta gaaacacctt
    44161 tcccagaaga tgcggaaaga agcgaaattc ataaaaaagc accagccttt gacgaattaa
    44221 gtacaagtac agaaattttg gaaacaggga tcaaagttat cgatttgctt gccccatatt
    44281 taaaaggtgg gaaagtcgga ctattcggtg gtgccggtgt tggtaaaacc gtactgatcc
    44341 aagaactgat ccataatatc gcccaagaac atggtggtat ttctgtattt accggtgtag
    44401 gtgaacgtac tcgtgaaggt aatgacttgt attatgaaat gaaagattca ggagttatcg
    44461 aaaaaacagc catggtgttc ggacaaatga acgaaccacc aggtgcacgt atgcgtgttg
    44521 ctttgactgg tttgacgatt gcggaatatt tccgtgatgt agaaggtcaa gatgtactgt
    44581 tgtttatcga caacattttc cgtttcactc aagctggatc tgaagtatca gccttgttag
    44641 gacggatgcc ttctgcggtt ggttatcaac caacattggc aacagaaatg ggtcaattgc
    44701 aagaacgtat cacatctacg aaaaaaggtt cgatcacatc gatccaagca atctatgtgc
    44761 cagccgatga ctacactgac ccggcgccgg caacagcatt cgcccatttg gatgcgacaa
    44821 ccaacttgga acgtaaatta acagaacaag ggatttatcc agcggtagat ccattggctt
    44881 cttcttcaag tgctttagca cctgaaatcg tgggagaaga acattataaa gtagcaacag
    44941 aagtccaaca cgtattgcaa cgatatcgtg aattacaaga tatcattgct atcttaggta
    45001 tggatgaatt atccgatcaa gaaaaagtgt tagtttctcg tgcccgcaga gtccaattct
    45061 tcttgtcaca aaactttaac gtggctgaac aatttactgg tcttccaggt tcatatgtcc
    45121 ctgtagaaga aacagtgaaa ggattccgcg aaatcttgga aggcaaatac gatgatcttc
    45181 ctgaagaagc tttccgaagt gttggccgaa tcgaagacgt cgtagaaaaa gcgaaaacac
    45241 taggctacta gaaaggggtg ccccatggat caatatttaa cagtaaatgt ggtaactcct
    45301 gccggtttgg tctatgatca ccatgcagct atcgtcgttg cacgtacgac agctggtgag
    45361 cttgggattt tgcctaaaca tgcaccaatc atcgttccat tgacgattga tgaagttcgt
    45421 gtaaaacgaa cagattcaga tacccacgtc gattggatcg ctgttaatgg cggaatcatg
    45481 gaagttcgtg ataatgttgt ttcaatcgtt gctgatagtg cggaacgtga acgtgatatc
    45541 gatgtatctc gtgcggaacg tgcaaaacaa cgcgcagaac gccaaatcga agaagcaaaa
    45601 gcaaaacaag atacgaacga attgaaacgg gcaactgttg cgttgcaccg agcaatcaat
    45661 cgtatcaaag tatctaagca ctcataaaat gagatgcgga tgtttatccg tgtttaagaa
    45721 cagtaaaaag aggttatgac agaagtggtc agcttcaaga cataagaagg aatttacgaa
    45781 aattgcttct caaatttttg tgaaatctca gcttattttt cgaagaagct acttctggaa
    45841 caccgtatat cggaggttgt gaaatcagcg ttttgtcctg agtattagag aaacaaatgg
    45901 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag atgattgaac
    45961 accgtttatt tggaataagg gactgcgaca agactttt
//
LOCUS       DACECR010000012        45667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.012, whole genome shotgun sequence.
ACCESSION   DACECR010000012 DACECR010000000
VERSION     DACECR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.012"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..243
                     /locus_tag="IWO17_000614"
     CDS             4..243
                     /locus_tag="IWO17_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289512.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            310..519
                     /locus_tag="IWO17_000615"
     CDS             310..519
                     /locus_tag="IWO17_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289513.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            579..1250
                     /locus_tag="IWO17_000616"
     CDS             579..1250
                     /locus_tag="IWO17_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0289514.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1303..3279
                     /locus_tag="IWO17_000617"
     CDS             1303..3279
                     /locus_tag="IWO17_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0289515.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3292..4047
                     /locus_tag="IWO17_000618"
     CDS             3292..4047
                     /locus_tag="IWO17_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0289516.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4044..4868
                     /locus_tag="IWO17_000619"
     CDS             4044..4868
                     /locus_tag="IWO17_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289517.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5157..7268
                     /locus_tag="IWO17_000620"
     CDS             5157..7268
                     /locus_tag="IWO17_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0289518.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(7306..9363)
                     /locus_tag="IWO17_000621"
     CDS             complement(7306..9363)
                     /locus_tag="IWO17_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ0289519.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9477..9689)
                     /locus_tag="IWO17_000622"
     CDS             complement(9477..9689)
                     /locus_tag="IWO17_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289520.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9876..10343
                     /locus_tag="IWO17_000623"
     CDS             9876..10343
                     /locus_tag="IWO17_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289521.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10340..10582
                     /locus_tag="IWO17_000624"
     CDS             10340..10582
                     /locus_tag="IWO17_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289522.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10600..10902
                     /locus_tag="IWO17_000625"
     CDS             10600..10902
                     /locus_tag="IWO17_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289523.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10974..13028
                     /locus_tag="IWO17_000626"
     CDS             10974..13028
                     /locus_tag="IWO17_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289524.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELGQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            13028..15640
                     /locus_tag="IWO17_000627"
     CDS             13028..15640
                     /locus_tag="IWO17_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0289525.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15637..18168
                     /locus_tag="IWO17_000628"
     CDS             15637..18168
                     /locus_tag="IWO17_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289526.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18220..18552
                     /locus_tag="IWO17_000629"
     CDS             18220..18552
                     /locus_tag="IWO17_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289527.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18553..19146
                     /locus_tag="IWO17_000630"
     CDS             18553..19146
                     /locus_tag="IWO17_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289528.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19309..21003
                     /locus_tag="IWO17_000631"
     CDS             19309..21003
                     /locus_tag="IWO17_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0289529.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21691..23568
                     /gene="ltrA"
                     /locus_tag="IWO17_000632"
     CDS             21691..23568
                     /gene="ltrA"
                     /locus_tag="IWO17_000632"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0289530.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23699..24076
                     /locus_tag="IWO17_000633"
                     /pseudo
     CDS             23699..24076
                     /locus_tag="IWO17_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24076..25281
                     /locus_tag="IWO17_000634"
     CDS             24076..25281
                     /locus_tag="IWO17_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0289531.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25297..25941
                     /locus_tag="IWO17_000635"
     CDS             25297..25941
                     /locus_tag="IWO17_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289532.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25952..26758
                     /locus_tag="IWO17_000636"
     CDS             25952..26758
                     /locus_tag="IWO17_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289533.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26780..27010
                     /locus_tag="IWO17_000637"
     CDS             26780..27010
                     /locus_tag="IWO17_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289534.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            27556..27831
                     /locus_tag="IWO17_000638"
     CDS             27556..27831
                     /locus_tag="IWO17_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289535.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27873..28295
                     /locus_tag="IWO17_000639"
     CDS             27873..28295
                     /locus_tag="IWO17_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289536.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28314..29906
                     /locus_tag="IWO17_000640"
     CDS             28314..29906
                     /locus_tag="IWO17_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289537.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29918..30253
                     /locus_tag="IWO17_000641"
     CDS             29918..30253
                     /locus_tag="IWO17_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289538.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30400..30594
                     /locus_tag="IWO17_000642"
     CDS             30400..30594
                     /locus_tag="IWO17_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289539.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30626..32998
                     /locus_tag="IWO17_000643"
     CDS             30626..32998
                     /locus_tag="IWO17_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0289540.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            33059..34519
                     /locus_tag="IWO17_000644"
     CDS             33059..34519
                     /locus_tag="IWO17_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0289541.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            34530..36734
                     /gene="topB"
                     /locus_tag="IWO17_000645"
     CDS             34530..36734
                     /gene="topB"
                     /locus_tag="IWO17_000645"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0289542.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            36973..37566
                     /locus_tag="IWO17_000646"
     CDS             36973..37566
                     /locus_tag="IWO17_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0289543.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            37582..38481
                     /locus_tag="IWO17_000647"
     CDS             37582..38481
                     /locus_tag="IWO17_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0289544.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            38484..38969
                     /gene="ssb"
                     /locus_tag="IWO17_000648"
     CDS             38484..38969
                     /gene="ssb"
                     /locus_tag="IWO17_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0289545.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39335..39595
                     /locus_tag="IWO17_000649"
     CDS             39335..39595
                     /locus_tag="IWO17_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289546.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            40036..40242
                     /locus_tag="IWO17_000650"
     CDS             40036..40242
                     /locus_tag="IWO17_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0289547.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40242..40493
                     /locus_tag="IWO17_000651"
     CDS             40242..40493
                     /locus_tag="IWO17_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289548.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            40508..40945
                     /locus_tag="IWO17_000652"
     CDS             40508..40945
                     /locus_tag="IWO17_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289549.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            40938..41645
                     /locus_tag="IWO17_000653"
     CDS             40938..41645
                     /locus_tag="IWO17_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289550.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            42026..42205
                     /locus_tag="IWO17_000654"
     CDS             42026..42205
                     /locus_tag="IWO17_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289551.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            42546..42809
                     /locus_tag="IWO17_000655"
     CDS             42546..42809
                     /locus_tag="IWO17_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ0289552.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            42803..43153
                     /locus_tag="IWO17_000656"
     CDS             42803..43153
                     /locus_tag="IWO17_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0289553.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(43177..43791)
                     /locus_tag="IWO17_000657"
     CDS             complement(43177..43791)
                     /locus_tag="IWO17_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0289554.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            44105..44527
                     /locus_tag="IWO17_000658"
     CDS             44105..44527
                     /locus_tag="IWO17_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289555.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            44537..44740
                     /locus_tag="IWO17_000659"
     CDS             44537..44740
                     /locus_tag="IWO17_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289556.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            44951..45535
                     /locus_tag="IWO17_000660"
     CDS             44951..45535
                     /locus_tag="IWO17_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0289557.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        16748 a         7210 c         9265 g        12444 t
ORIGIN      
        1 tagatggcgc gaccagtttt agaggcgatg gatatggata ctttgacaaa aggaaggtat
       61 gtacagctgt tagctcgtgg ttattcacct aaagaagtat tcaaagcctt atctaaagga
      121 acagatatgg aaaaagaaag attgcgaaaa gaattcgatt attggagaac ccataatgga
      181 atcaaggatt tgaaaccagc acggccagta attaaacgaa agaaaaagat aacccaagac
      241 tgaagtgtag agcgtatgtg atcataggct tttttatttt tgcaaaaaac aaggaaggta
      301 ggaaaactca tgaattatga agaaatcgaa aacagaaaaa aagtgtcgaa agaaatggaa
      361 gaaaagttac taaaaacgat gaagcaaaaa caccttaaaa gattatctgt cgcgcaatat
      421 atcaatgata tgcagttaac aggaaaagag aaagcttgtt tgttaggttc aatgaaaaac
      481 tttgaacagt tgagacgaac ttatagaatt catttttaaa actttttcta tggaaatgag
      541 aaaaagtttt tttgttttgt ctgaaaggag gcagatggat gaaaaaatgg ttatttggtt
      601 ttttaggagt cactttaatc gtcttttgca gtgtgtttgg ctatgttagc taccaaaaac
      661 atgaaggaga ggtgtttaag caaaatatag aaaaaaagat gcccgtagat caaatcaatg
      721 cacatgcaaa gagttataag gaggatgcaa cgaacgtcaa taatgatatg tctttggggc
      781 aaatgttgtc catacaaaaa gaagcaatcg aaatgggcgt caataaacaa gtatttgcac
      841 aaattcagat tcctgcactt ggtttagcat tgccaatttt taaaggggca aatcaataca
      901 ctttgagtct tggagcggct acttattttt atgaagatgc cgaaatgggc aaaggaaact
      961 atgtcctagc aggtcataac atggaaatgc caggtgtttt attttctgat attcaaaagt
     1021 tgtcattagg ggaggtgatg gacttagtta gtaacgatgg cgtctatcgt tacaaggtaa
     1081 cgcgtaaatt tatcgtacct gggtacttta aattaatcga tggcgttcct gaagagaact
     1141 ctttcttgtc tttgccaaag aaaggagaga aacccttatt aacgttgttt acttgtgttt
     1201 atacgtcaca aggaaaagaa agatacgtgg tacagggaga gctacaataa caaggtttta
     1261 atcaacgcga gtcgttgaaa tatgaaatga ggagagtaaa aaatggcaat aaatcggtat
     1321 gtaaaaaaag ctggaatgct tgttggaatg ttaggaatca ttgcaggctg ctttcagggg
     1381 tatcaagtac atgcagaaga tgtcacgcaa aaaacaccac cagagaaggt caatatcacg
     1441 gttcataaat taatgtacga tcaaggaacg caattgaatg ttgatattga cgggatcaaa
     1501 aacgatggtt atacgcacga tcaatatcct acaggtgtaa cgaaatacaa caaggcagat
     1561 tatggtgatg ttgagtttac gttaggaaat attacggatc aagtattacc gagagaagat
     1621 tctgatctaa ccaacgcgaa agtggacgag attgtacaag atgtagaaga caagggatct
     1681 aacagtgagt acgtcaagaa tgcaaccaac aaaattactt cagctgtaga tgaaaatggt
     1741 gagatcactt ttactgatca accagcctat gtcaattcca aagggaatgt atatgtggtt
     1801 tatgaaagta aatccgctgc tggattagtc acacaaaaag caaaaccaat ggttgtgatt
     1861 gcgccaatga cagataaaac aggatcagga tttttaaaaa atattcattt gtatcctaag
     1921 aacattgttt ctaagttgag ttttgaatta acgaaatttg gtgacgatgg cgcagaccaa
     1981 agcaaacaaa cccttttaaa aggggcaaaa tttgagttat acaagggcga acctggaaaa
     2041 ggaacaaaat tgggtgatct tgtatcggat gatcaaggaa agctgactgc gactgatttg
     2101 acgttaggaa aatactattt tgtggaagtt ccttcggaag ttgtagtggg atcagatcaa
     2161 gaaccaacag cagatcaata tttattaggc gcagacgctc gcaacgatgc gcacaataaa
     2221 ttaacctttg aaattacgaa tgatggcgtg acaagtgact taaaagcttc atacgtaaac
     2281 tataaagcac cagtgatcga taaaacagta acaaatggaa caggacaaga acattctttc
     2341 caaattggtg acgcagtaaa ttatcaagga acgattcata ttccaacaga cattgcaggt
     2401 ggcgcagacg gtatcacagt aaacggtgtg aaaagcgaaa cttctcctta cagtgtattt
     2461 aaatggggag atacagcagg taaaggactt tcttatgttg ctgaaaaagc agatattaaa
     2521 gtaacaaata atgatggttc agtcgtatta aaagaaaata ctgactataa aatcgaaaat
     2581 tctgatagtg gttttgtgat cgattttatc gtaaataatg gacaagtaag tgatacagtt
     2641 gcgaatttac atggtcaaga tctaaaaatg acttataaca tgtttgtgaa tgatagtgct
     2701 gaaatcgcaa atccattaac aaatagcgtg gatttcgttt ataacaataa tccatttaat
     2761 caggaagaac accacgaaaa aacgaaagct gacgttgtga cttatggcgc aaaattctta
     2821 aaagtcgata gcggcctatt tggaacaggt atcaaagcaa caccgcttga aggcgcagaa
     2881 tttgcagcta agaatgcaga aggtaagtac tacggtggac ttactgatac agataaagat
     2941 ggtgtcaaag aagcagtttg ggtagacgat gtagcaaatg cagcaatcct aaaatcagat
     3001 aaagagggtc attttgaaat cactggttta actgaaggag attactcact ggaagaaaca
     3061 aaagctcctg aaaactatca aaaattaaca aaagagatct ctttcaaagt agacaaagat
     3121 tcatacaaag aagaaaaccg aatcacgatc aaaaataatc aaaaagcttc cgtaccaatg
     3181 actggtagca acggtttcca atcttacgta ttgatctctt gcttgttgct aggagcaggg
     3241 ggactttctg cagttgttta ctttaagaag aaagcctaga aggatcagct aatgattatt
     3301 cgtaagcccc caaaaagaag agtcatgcac aaactgatga tatttttttg gttgctgtcg
     3361 gcagtagcag tctatttaat tccagttgtg gaaacaaatg tagcgaaatt tcaacaacag
     3421 caaagaattt tagccattga aaatcttgaa aaaacagatt cttctttgga tgttgcgaaa
     3481 atagagttag gcgatcctgt tggtgttctt acaattccaa gtatttcttt aaaactacca
     3541 atttatgacg gaacttccga caaaatttta gagaatggtg tagggattac ggaaggaact
     3601 ggcgatatta caggaggaaa cggtaaaaat cctttaatcg cggggcacag tggactttat
     3661 aaagataatc ttttcgatga tttgccttca gtaaaaaaag ggcaaaaatt ctttattaag
     3721 gtaaatgaag aacaacatgc ctatcaaatt tatcgaattg aagaagttaa agcagaagaa
     3781 ttacaaaaaa attatttaga ttatcttatg cctgaaaaag accaagatag gatcacgtta
     3841 atgacatgca caccaaaagg tgtcaactca caccgctttt tagtgtatgg tgaacgagtt
     3901 tccttcagta aagaagattt aaaactacag aaaaaagcaa aaaataggaa atcaatttca
     3961 ttggattggc gattagcact gctattcgct tttttttgcg ttcctattat ttttatttat
     4021 aaaaaaataa ggaggagatc ggaatgaata agagaaaatg gctgtttgtt tgtgtagcag
     4081 ctctcttttc tactgtagct tattcgcaac tgtctttcgc tgacagcagt gatagtagta
     4141 ccactcaaac aatagataat tcaattactg caccaagcga aacggacagt tcggagaata
     4201 ctacagaaga aacggcagat tcaaatataa gctttactga atcaacgaat ccaacgtcag
     4261 aaagttcttc agagacttca gaaaagccgc aagacgtaga agatacgcaa gtgatcaagc
     4321 aagccattca tattcaaaaa attatttctt cgaatcaaat caacaatcaa ggcagtcaag
     4381 tagcccagca agaaggtatc aatggcgcga agtttattgt ctacgatatc acgaatatct
     4441 tagaagaaat gtcacaagat gataatgaga aaaatcaatc tgtagaaata attgagtcaa
     4501 aattgaaaga tcgagcaaaa aaactttcat acgaccagtt gaaaaaagtg gctgaaggag
     4561 aaaccaaaac gattgatgat caggaaggcg tgattgaatt ttccattgaa gtacctgcaa
     4621 acaaaaaaca agcatactat atcgttaatg aatcaagccc tgaaaacatt tcaaacagtg
     4681 aagatattgt tttattgaca cctgtttcaa atgaaaaagg tgaatttttg acggatgttt
     4741 ggttataccc taaatcagag aaatcagagc cgaaagaaga aattaaaaaa gttgtgtcaa
     4801 ctggtgtaaa gaagaacttt tttgaaaatt gttgggaatt ttttgctcaa ttattttttg
     4861 ataattagga tgaaatgatg aaaataataa gaatattagt ttttaatcta tcctttttag
     4921 ccgcaacaat attgtgttgc ggtttaattt tttctcaatt atcaatttat ccaagatatc
     4981 aacgattgtt cattcatttt ctaaacattc aatgtgcttc catgccatta gaggtgatta
     5041 aaaagctctt gatcaatcca atttttatgg ttagtttggt tcttcttaca ggattggtaa
     5101 cgtgggtatt aggaaaaaaa gaatgaaaag cttacgatcg tttaaaaaaa tattagatgg
     5161 agggcattat ggaaaataaa attcattgca ctacgggaag agtaattact ttagtgttgt
     5221 ttttgttcct tcttcttttt gcgaggggaa ctgcagtcca ggccttagaa aaagttgatc
     5281 cgagtcaaat caaagaaaat aataatctgg tgctttatca tgatagtgat tcattgagaa
     5341 atatcgaagt aactgggatc aatggattta agatactaga tgagtattca actaaaaaac
     5401 aaaaagtact tgcttggcca aaagtcaaag atttgaaaag tacgtcaaaa atcgttgccc
     5461 attatcaaaa agtaggtact tatctaggaa aagaaattga ttgtgacgtg actttttcta
     5521 attttgtagc gcacgctggg gttaaccaag acggattagc taaaacaatt aatggcaaaa
     5581 agtattataa ttatttgctc tttgataaaa atatttatcg aggatttttc atgcagtcaa
     5641 tcaatgtcat gaatgcaaaa ctagaattta agtatgcagg aacaaaaaca aaagtagctt
     5701 tgagtgaacc acagccagaa attcaatcaa aaatgagatc gatgagagca gctggtattg
     5761 atttaaacga tacatttcta tccatttaca gtttaaatgg acagtatcag caacgtgtgt
     5821 cttggtataa tgacaataat gaatctgtcg gtgaacgtaa tattgatatg agagaatttg
     5881 ttgggtatga aaaaatgggt agcttacctt attttgtcac aacagataca gcatgtgcag
     5941 aatacaaagc tcctgcgtta tcaacaaaca atttaacttc aaaagtagtg ggaggacgtg
     6001 cagaaaaggc ttatagctcg aatgatcatt tcaccgatgt tgtaggagct gatacttatc
     6061 acagaagtgg tgtaacgtat acgcttcaag gcgcttcccc aacattcatg attggcgcaa
     6121 atacgaatag tatgatgttt agctttgata ctgcattgct atggacacca caaccatcga
     6181 agcctacaaa agaagtgttt aacaaagcta atactgaaga ggcagcacac aatattgaca
     6241 aaaaagtgat tccacaagga tcagatgttt actatcatat tcaccaaaag tttgatgcat
     6301 taacagtcaa cacaatgaac aaatacaaat catttaaaat cactgatacc tttgacagca
     6361 aaaattttga tatggtatcg gatgggaaaa actatgatgg cgcattgtgg tatgttgaag
     6421 cgaatggtac ttggaaaaaa gctgatacag gaggaaagtt aactgtatca ggtaatacag
     6481 tcacttatac tgcttctgac agctttttag ctaacgctgc aaattacggt agggattata
     6541 ttttgcagct ccatatgaga gcaaaaaatg atgcaaaagg aattgcacca aatgtggcta
     6601 ctgtaacgtt taacaatgat ccgaacaaag gaacacaaaa aacaaataca gtagaaaact
     6661 atttctctct taaaccaaat aaaaaagtgg aacaagatgg gaaagatgtt gatggtcgaa
     6721 atgatggtaa aaaaggaacg cctacagctc ctttaaatgc aggatctgaa gtacaatatc
     6781 tagtcacaca aaaatggcat acaaaaggtg tagacattgc ctcagatcat tacaagcaat
     6841 ttagtattca agatccagtg gaagatcgtt tgacgtataa agaaggttct gcgcgagtga
     6901 tagatatgag tacaggaaaa gatattactt cagaaggtac actaacctat gatagcaatt
     6961 caagaacgct gaagtgggaa gcttccgctg atttcttaag taaaaatctt ttagatggac
     7021 gagaaattca gctgatattt acagctaaaa ctcctttgaa tgaagaattg gatatcgaca
     7081 accaagcaaa agtttcagta gaaagtattg agaaagcaac taatgtagta acgattgggg
     7141 taaaacctaa cttacctcag atcattgtgc caaaaactgg atcgaatcat ttagtgctaa
     7201 ctgttattgg aagtatatta tttcttactt tagcatcatt gggctatgta gttttaaaac
     7261 tcaaataaat ataaaaacaa ggctgagaat cagccttgtt ttcttttatt tgaagattgg
     7321 attcgcacta ataaatacat tatctacgcc aattctacca gcagagtcaa ctacaaaatt
     7381 atgacctaat ccgagaatac tttggttatt ataaagtaaa tttgcattat gggcagggct
     7441 atttttccat cctaccatgg catcgatagc accatcgtct gataaaaggt catcaatata
     7501 cagcccagtt tgacttatgt tttctccctt agcaccgtag cagcctaagc tttccaataa
     7561 atcttggaaa gtaccaccac cagggcgata gtgactgaag ctgacttgta attctttagc
     7621 tcgaatatct gcagcctgtt gaacaatatc attgaattgc attggggcaa caccattttg
     7681 ttggcgataa tcatttaacc aagcaagttc tttttgacgc ataaagttac gtaattcgtc
     7741 taatgaaatc tttttatatt tgaatgtgat tgttttattt tgatcgacgt tattaatgga
     7801 tcgttggccg tttttatcca cggtaacggt aggatcgtca tttctgcaca agaaataatt
     7861 ttgatcagtc atatttttcc aaggagtaga gaagctatcc ccttcgtata ctgtttttga
     7921 ttcactagag agaacagcgt tggtatcagc atttacatga ttaacagtca cattaaattt
     7981 acgagtgtag ttgaaaatat aattttgact ttcagtcaca ttgtttaagg ttactgaatt
     8041 tgttggttct acccgatcgt ctaaaacata tccttgaata gaaggagcag ttaaagtgac
     8101 actttcaccg tgagtgacgt tgattatctg ttctttcaaa atatcaactc gcccctcatt
     8161 tttgtagttg ttattgcaca acaaattgat ttgatgcact tgattataat tgaatgtgat
     8221 tgttgtatta cctgtgattg ttttgctttg agtatcatta ccaatcactt tgtagctaaa
     8281 attatcttta gcaccttcgc tatatttaaa tgcttttgcc ttagcagtgt aagtagaacc
     8341 agaagtcact tttgcttgat ctgtagcaat cacttgacct gtttgattgt caatatggtt
     8401 aatggtaacc atatattctt ttggcgtttc aggtttgtgt tgcaaccctt taaatgcagc
     8461 atcaagacta tttttagcat tgtttacttg tgtttgagaa accttagaat cgtctagtac
     8521 ttttttagca ttggataaag cagtctgaaa attattccaa gattcttctg taaaatctcc
     8581 tttagcagtt gattttactc ggttgtataa agcttgtaat tcagctttat tcacagtagg
     8641 aactggtttt ttctttaatc ctttgtaagc agaatctaat ttatttttgg cactatccac
     8701 ttgcttttga gtcgcttttg agtcatttaa cactttctta gcattgttta aagcggtttg
     8761 gaatgcgttc caggtattgt cggtatagtc accttttttc gttcccttaa cgctcgtata
     8821 aagagcttct agagccgatt tattcacttt gggtttagag gtgtcagcgc cataccaacc
     8881 gataccttcg tatttccagc catagtcttt ggttaaccat tgtacttctt tccaacttgc
     8941 agtgtagtga tgtgagccag attttacacc aggaaggaaa aggcggtaga tcggtttatt
     9001 atttttttcg gcagaacgcc aggcaggccc ttcataagtc cagccgtaat ttttagtcaa
     9061 ccatttcact tcatcccaac tagctgtgta atggtgatca cgaagacctt tattgtacaa
     9121 acgatagaca agatggccac cgctagtcgg tgcttcccag gcggggcctt cataatttcc
     9181 ccattttatc tttactagat ggtcacgttc ttttttgcta gaggtgtaaa aatgttcatt
     9241 attatttgga ttgtaaaggc gatacatgac gttagttgaa gcagcgtcaa cagatccagc
     9301 aagactaagt gaggcagcaa atgttccaat agctaaaaca gtttttggat taaataattt
     9361 cattttttca actcctaaat ttaattaaaa atgtttacat gattattata tatcggctaa
     9421 ccgatatagt caataaaaaa taagaaaagc cttatataat tttggctttt cttattttat
     9481 ttgttagttg aagtattaat atcaacgatt tcttcagcca atttatttaa tttcatagca
     9541 tttatgaatc tcatgttgtc tatactagag cgtttgttgg caaggtcact tactgtagtt
     9601 aagggtacgc cagattctat ggatatagaa cgaactgatt taccactatc aaacagcaac
     9661 cattcgattt tctttttatc agcgtacatt tcaatgctcc ttcttgtttt ttctttatta
     9721 tagctctttt ttgtaatgaa tcacaatatc aagagaaaaa ttttctatag aaaggtggat
     9781 ttttatgagt attcgttctc cgtgtatcca aatgaggttt gttgctactt tcgttatttg
     9841 atatactaaa aataaacaac gaaagaagga ataaaatgaa gaaaaagcaa ctatggtttg
     9901 tctctatgtt tgcaatattt tttattattt taagtggatg ttcaagtaat aagaaatcgg
     9961 aagctaaaga agataatagt ttattgcaac caggaactgt ttggaaagaa gaagtaggtg
    10021 gtcttattca taatttaaaa attatagatg atactacctg ggaatattct gaaagtgtat
    10081 ggcatccaga tcctgtacaa ataacagtta agaggcaaaa agactatgaa ggtttagaaa
    10141 gatacaaaat agtagattct gcaggcatta gggaatttat aaacaaacct gattcattat
    10201 ttattgttgt tccctatgag aaggatggcg taaaaaaaat aatttttctt ggatctagca
    10261 aggatgagaa gcgaacgaaa gagaaattaa ttcatgatgg atcagagtta aataaataca
    10321 aattacaaaa gactagtgag tgattgctag tctttttatt tatcctgaag gaggaatcac
    10381 agtggcaaaa gttaaaacac cattcgaaaa agccgcgtct aaaatcttag aacgccgtgg
    10441 tgtttctgaa atacagtgga aaaaagaagt tcttgaaaaa gcacaatttg acctattacg
    10501 aggaaaagat aaggacttag aagaatatat ctatcaacgt gaacgtgaaa aacttgtatt
    10561 cgatgaaatt attaaacatt aaaaggagcg aataatctta tgtttgaatc aattacaatg
    10621 aattttttaa ctcaaccgaa agtagtagct gcagttgata tttttagagc attacaaact
    10681 gccgcaacta gtttgaaaaa taatctgatg atttttgctg ctgttgcggc agtggcttcc
    10741 attgctcttg gaggtatttt cttcttcttt gggcgaagag gagctgaaaa cgggaagaca
    10801 cacgtaggtt atgtattgtt gggggtagga gttattttag ctgccgcttc tattgtagtt
    10861 tcaatgttct caatgtttgg acaaacccca caaggactat aaaaaaaaca agtaggagga
    10921 gtaaaaagta cgtggctact ttttactcct ttttttatga aaggagaata ttcatgtttt
    10981 ttacgatttc aggaaatgca aaattggata aagagttaat aagaaaagca acgaagaaag
    11041 tgggaaagcg tacttccttt ttaccgaaaa caattaaata ttcggtcatt gaaacggaag
    11101 aacagctaca tgtgttagga caagtgttag aacaagagaa tcaatcttct gttaaattga
    11161 ctctcacgtt acatgataaa gaaggaaaaa tagaaaaggc tggcacagaa tacgtttttg
    11221 gcccactgga ttatcaaatt tcagaaggtt tttatcctac attgatagat ttaatccgcc
    11281 ttgatgtgtt taaagatacg gatgaaacta tgagtgtgga agagtcttat cgaaacgaaa
    11341 aacttttaca agttttagaa gaagagttag agagcgacca aagaaaagtg aatcaagtga
    11401 gtgaagtcga agtaaatgaa gaagtaccat gggaacgacc aatatttcca aagaaggagt
    11461 caaaagaaaa agagacagtc tatgttgacg aacaagctga tattccgact cagaaatcga
    11521 tatttgagga agagaacgat gtgatgggtc aattggaggt agatcccttt gaagaaacaa
    11581 tggaggatga agagacggac gaagtagaag ttgatccagt tgaagctaat gaagagtcaa
    11641 catggactga aggggatttt caggaatata ttgaaccact tatcagtgaa gaagtagaaa
    11701 aaaacgaaac agagatacaa aaggcaactg tatttgatta tattttggat caatatccgc
    11761 cagagcatga atggatgaaa gaaaaaaaca aattattttt aaatgagctt tattcgaaat
    11821 atcaattacc ccaaacctat caagcatttt tagagagtaa agaagaatta atctcacagg
    11881 gtaaaggcac acttttagaa cagttagagt ctgtccaatc aacaaattgg catgagcttg
    11941 gacaagaaga tttattagcc acctttgaag aacgtcaaca agaaacagat caagaaatta
    12001 atgagtatgc gacggaacag tcgtctaatt gggagaaagt aaaagaggaa ctaaataaag
    12061 aagaacagcg agtgattgaa gaagaagtac aaaaagtcaa agagcggtac gaaaagaaac
    12121 gcgacaatgc ttattccttg tgtgtagaaa agatcaacaa ttttactaag acgaatcaag
    12181 aagcgttatc gaaagaaaaa gaaaacttgt taaaagaacg tattaaagaa cgaaaagaag
    12241 agttctaccg cggtttacgt acagataagc taaagattag caaagaatta aatgaacaac
    12301 tgaataagct atatcaaaca acgactgaag ttcttatgga aaaacacgaa cagatccagc
    12361 aagaattaca agtacaaatt ccaaaatgga aaaaagagta tgaaattcaa caacaagaat
    12421 tgaaagaaca agaagaaaaa caacggcaaa aagaaaaaga gcgtgaacaa ttagaaataa
    12481 gaaaacggga acaggatctt gcggaacaaa aaatggcgat agaaaaacaa cggttagagc
    12541 aagaaaagga aaaagaagca gctgctcgta aggaacgtca agaacaaatg gatacattgc
    12601 gtcaagcaca gttgaattat ctagcacaac ctcaaccagt agctgctgtt caaacgccag
    12661 tgaacaacca agcaaattta actccttcac cccagcgttc ctttaacgga tggatggtgg
    12721 gatgtattgc aagtttaagc ttgcttctag gtggaggaac agtatttgct tttaatcata
    12781 tcaatcaatc ttcacaggct gattctgtag ccacaacggc gtcaatcaag gaagaagccc
    12841 aggaacaagc aagaaaagag tatgaacaac aattagaaga attattgaat cgtaccaatc
    12901 agtcaacaac agagtcaagc atggaaaata gcaaagagag tgattctagt aaggctaaag
    12961 aaggatcaag cgattcaact gtacaatcaa acacttccga aagttcagaa tcaaatgagg
    13021 tgaaataatg cgtgttagag aaacaacgaa gcgaaaagac aaacgattca atttagaaaa
    13081 gaataggtat ctttctcatt tctatcgcta tactccctca ggagaagagc taccagaaaa
    13141 acatgacaaa cgcatttcga aaaagatttt ttatagtgga cttgtatacc tccctttaga
    13201 gtatttagta acaagagtac cagaagtttt caaccgattg gaaattgtta agggaagctt
    13261 acaaggaata aatctgtttc aattacagga aatgccacag gtctttcatt ttgattcagc
    13321 tggtttttgg aatccattta ctcctacaac tagtttgggt acacgtttag caatttcgac
    13381 agtttgtgtg gttccagctg tacttatggg attgaaaatg gacatgtggt ttcgtcctct
    13441 agcagatgga caaaaagggg ataatcgatt aatgacctta aaagaaatcc aacagattta
    13501 tccgcaaata cctgaaacaa aaactacttt tccaggttat ggaggtattc caatcacaca
    13561 ttacaaaaaa tattggtaca ttcaaacaga tactgttaat acgtgtattg ttggtacctc
    13621 tcgatcaggg aaaggtcaga ttgaagtgtt agccaccatt gacaatttat ctcgtgcaga
    13681 aaaacaaagt tcaatggtcg ttaatgatcc aaaaacagaa ttatatgttg gtagtaaaga
    13741 tgaactagaa gctagagggt atgatgtata tgcctttaat atcaccgatc cactgcaatc
    13801 aatgagtgat aatccattgg cactcatcgt gaaatactgg aaacgaggag acatagatac
    13861 tgcaacacag ttaaccaata ctttcacaaa tacgatttat tatgatgcca acgcttcaga
    13921 taacaaatac tttaacgaaa acgcgcagaa agctgtcaat gcgcttattt ttgcgttgtt
    13981 agaacaagct gatcaaacag gagattactc caaattgaca ccgaataatc tcgttgagct
    14041 tctttctgag attggcggtt ttaattatta tccagatcca gacaatgaat tcaaacagtt
    14101 gaatgcctta gatgaattta tgaatcagtt gccacctgga aacattgcaa aaaaacaata
    14161 tggggcaacg aaaattggat ctgataaagc gaagggaaat attttatcaa cggcaatcac
    14221 gggattgaat ccatttactc taacgaaaat tgcaaagatg acttctcaat caacgattga
    14281 ttataaatcg gttgggtttc caaaatactt agatttgaaa ttacatgaaa gtcttttaaa
    14341 tcaacgagtc gtagtagaat tttatcataa gggaaagaaa tttcatgaag aacgtgtcaa
    14401 agtaggctat aaaggattta gtgaaatcaa ttttgattgt catttagttg caggtgatca
    14461 agtttgttta aaatacgatc atcaaggaaa agaatatgtg gcttggtatg aattttctca
    14521 aagagtattg aaagacgaag agggtaatat cgtttatcgg aaaaaacgag gagaaaccca
    14581 tttgccagaa ttagaaaaag aagccgttct aacattagat gaggaaaaaa gtaacttatt
    14641 agtagagtct attcggatgc actataccga taaaccaatg gctattttta tgcttacacc
    14701 tgattatgat ccatctaacc atatcattgt tagtattttt cttagtcagt tatataaaga
    14761 attatctacg caatgtgtta agactaaagg agataaatgt caccgcagga ttcattttat
    14821 tttagatgaa tttgggaata tgccaccaat ggataatatg gaaggaatca tgacggtcac
    14881 agctggccga aacatgttgt gggatctgtt tattcaatct tatcagcaac tttatgcgaa
    14941 gtatggagaa caaaacgcag cgattatcaa agaaaattgt cagacacata tttatattat
    15001 gtcgacagat gataatacca tcgaagaatt ttctaaaaaa gtggggaata agacggttga
    15061 acaagagaac agtaccatca attcattagg tatgaatact catatcaatc gcaatgttga
    15121 ttcagatcgt atcttaactc ctgaaagaat ttctactttt ttagaaggag aaacgattgt
    15181 actcgctcct ttaaaacgac aagatttgaa aggtcataaa atacggccgt atcctatttt
    15241 taatcatggg gaaacgaatc ttccttatgc ttatcaatat attgaagatt tcaatccatc
    15301 gtgggatatc aatgatatag acatacattg tacacatgcg gacttggatc ttaaggcttt
    15361 acaaattgat tatgattatt tcctaccaga tgaatatgct agaaatctct atcatggact
    15421 gcatgatcaa ttagaagaaa aaaacgaaga aaatatgttt tataaaatcg ttcaatctct
    15481 tgagaatgag acggctaaaa aattgttaat tcaattttac gaagaacaag cagcagatcg
    15541 acttcaagaa tatgttgaaa agttgatcaa aaataaagaa atttctgaaa tgattgggaa
    15601 acggttgtta ttggaatctg aaaaggtggt gaaagaatga tcattggagg aattttttct
    15661 ggcatagccg actggtggtc aggcttattc ggagaagggg atgcaaaccc caaaaccgta
    15721 acgaaagtct tacaagatta tgggaattat ctgcagtatc aagatgtatt tagttggatt
    15781 tggaacactt ttctgtgggg attgattaaa ggattatata atctcaatac tttactagaa
    15841 aaaaccattt atcaaagctt tgatttaaaa gacttattga acgccgctgg ggtaaatgca
    15901 ctatttcaaa gtttgattag taaggtattg gctctttttt gtgtcgtcac attaatttat
    15961 ttaggcttga aattttttat gagtaaacac ccaccaaaag tcaaaaatat cttggttaat
    16021 ttatttatgg ccatgatctt gatttttggt ggtagttctt taatcgatga agggttaaat
    16081 atcagtaaaa gcttttacgg agacgtcact gcagcaaata aagcagataa aacgggatca
    16141 cctgcttttc aaattattca aaataatgtg atcgatgtca gtacgttact agcaactgac
    16201 ccgaataaag tagaatccct accaacagat aaaagaaatg ctttaacagc gaaaaatttt
    16261 aaaacagcga atatcaatgg gattattact cctgaacaag ctaaaggtat ggcaggtaat
    16321 gataaaagtg ggttatcaga tatacaaaag gatcggttaa aggcattaca ataccgattg
    16381 gaactagatg ataatgggaa agaaattcct gttcaaatta gtgacgaagg cttagcaaaa
    16441 tatgtctata cttcagggta tcgtcgatac agtagtaaac caggtattat tttagctggt
    16501 gaatcttctt tggcagtagc ctatttattt atcctattta cgatcgttac ttgcattatt
    16561 gagctggtat ttaaaaaatt ctatttagtg attgccgcgt caacggactt tgagacaggt
    16621 caacggatga aaagtgcgat agaagatatt tctcaatcct tcttgttatt ggcgtttacg
    16681 attttagaat tacgaatcta taccttaatg ctttcaggta tcggtgattt gcatgcggca
    16741 ggaaagatca atggattctt gtatgtcgtt gctttgattg tactaaccat tgcgctattc
    16801 aaaggctctc aatcagttac gaaaattttc ggcgttgata caagcctgaa aaacggcagt
    16861 aattctcttc ttagcatgtt tgccttagga aatattgcga aaaatgtggg tagcggagct
    16921 aaaaaccttg caagtgctgg taagagtggt ttaagccatt taaatgatat tcgtaaaaat
    16981 ggatttggcc atcgaaaaga tgttacccct gataacgggg aagaacaatc agacgataaa
    17041 gctacaactg tacctaatag taagaaaaca gtttccgaaa tgttcgggaa agcgaaaaat
    17101 ggtgtttcta aagccgcaga aggtcttggt tacgtcaatg aacgtgggtt aaaaggaaca
    17161 gctgaagatc ttacagaaaa agccaaagac accttagaag ataaattaga cggcacgaga
    17221 gctaaagaat tggctgaagc tgtgaagaat ccatccgaag cactagaaaa acaaaaagaa
    17281 aaagcttcag aagtcaaaga aggactaaaa gatgcgtatg gtgaaggaca agtcaatgca
    17341 gcagtgaaag ctaatacaac aaccgaaaaa gaaaatcaag aaggtatagt gattccttca
    17401 gtcaaagatc accaagaagc gttgcactct aatgaaaatt cgattgaaga gaaaaattcc
    17461 atagaagaaa gaaattcaga agaaggaaaa aatggacagt taccaaaaac tgatccagaa
    17521 caatatcgct ataaacctaa cagtgacctt gaaaatgaga agcgaatttc agatttatca
    17581 aaagtaaatc aagaagacaa agcagaaaat gagggacagt taccaaaaac tgatccagaa
    17641 caatatcggt atagatctgt taactccgaa aatgaaaaac gcatttcaga tttaccaaaa
    17701 cgaaatcaag aagtacaaac ggatgaggat tccatcgttc tgccaaatga taatggaatc
    17761 gaagttggaa ctgaaaaatc aacgaaaatt ggtttaccta aggcacaaaa tttaccaatg
    17821 aaagatagtc gtttgaatca attcaaggat aatgatcaaa gttttgttca tcaatcacct
    17881 gcaggtactg ttcaaatgaa accaagtcgt ttgcctgaaa atggaaatct gacaactgtt
    17941 tcttacaatt tatctgaaag tggtgagaca gggaatgcgt ccgttcaaaa tgttgtgaca
    18001 agagaagctg caccaaaacc tattcaaaac gttcatacag gttacaagcc agcaaatgtg
    18061 gcatttactg caccaacaaa aataaatgtg acaagagaaa caaaaaacag gaacaatgca
    18121 ttgaaagaac aactattgag ggatatcaaa caggatatca aaccataaaa ggcctacaag
    18181 ttaggccttt tttctatgta tgaaaaggag aaatcattta tggataaata tagtaacatg
    18241 aaaaaccctc caagatttag tggagaatgg aagtttttcg cttttttctt tttagatcta
    18301 ttaacgcttg gtggctgctt tttttgggcg aaaatggtca ataatcttgt ccacttacca
    18361 ctcttttttg cactcttgaa ttatggctta tggattgcga tagcgttagt gattgtttgg
    18421 cgaccctata ataacccagg tcaacgacaa atttttgcga ttatcgagtc tatttttttt
    18481 gcggacaaga accattatca tgcgttagat cctaaccgaa attatcatgg tacgtataag
    18541 gaggatgaat aaatgcgagt caaaggacaa aattcagttg aaaggaaatc cattttgcag
    18601 tttggaaaac cagaagagcg ctctatcttg gacttatttc catataaatc ctgtacagaa
    18661 gagggagtct taattactag agaagatcat tttcaacgtt attatcgtgt agtttctaca
    18721 gacgtggaag gattaaatga acaagagaaa ctagaacgaa tgaatcagtt gaccaccgtc
    18781 atgcgtacgt atgtgccaac aattaagtta gttagtttga ccacagagac ggatctaacg
    18841 gaacaaatcg tgcaaaagcg gcaattatta gataaaaatc gtatggaaca gtccttagga
    18901 agaaatctac aacgattaaa aaaatatgaa aagaaattag tcgaagaaat ccaggaattg
    18961 aaacgagcag aacaagaacg tcctgactta agcttctttt tcgtcattga aggaaagaca
    19021 atcaaggatt tacaaaccag aaaccgtcag ttaatgcgat ctagtggcat tttagggtta
    19081 aaaccactct acaaaaaaga attgatcaag attctgtatc gaatgaacaa tatgaatgat
    19141 gagtaggaat ggagaagaga gaatgaaata tccgttgata tggttagaaa aagatgttcc
    19201 gaaggaagag accactagaa aatttcctca cactttaagc ctgggtaagt tgggaaaagg
    19261 gcaatctttt aaccaagtaa agaatcaaac aagaagaggt gactgtacat gttagaagca
    19321 attaaaacag ctttttcacc acaaaatgat caaaaagtac ttgatccatt aaagcaacta
    19381 ggaaatcggg aaatagaaat tgtaaaatcc aaagggtatg atttagattt tttaaaacaa
    19441 gtgatgccac aaggtgggtt aaattttgat gaagaatatg tgacgtatgg taatggttat
    19501 tcacgatgtg tgacagttta tcactacaca aaaaatccta cgcttttttg gttggccaat
    19561 ctcatgaaca atcctcatac agtggccaca ctcgatgtta gcacagatga aaagcaaaaa
    19621 gtgattgatc aaatcaatcg tgccttagat gaattaaatg atcgatcaga taatgaacga
    19681 aaagcgactg accgaaataa aagcgcggaa gaatacctgg aactagcgga atatgctaga
    19741 aaattgactt ctaatgggga aatttctaag caagtcaaag tgagagtaca tgtttacgga
    19801 aacacgattc aagaagtaga tgaacgagcg caggaactga tcgaaaatct caaagggatg
    19861 gattatcgtg cggtgactta tctgcataaa accaaacgag attggcaatc gatgtttacc
    19921 atgttcgatg aacaagctga atggttcgga aatatcaaag ggcaatcact accatcgtta
    19981 aacattggcg gaggtttccc gttccaccac caaacgctaa aagatccagg agggacacaa
    20041 tatggggaaa ccatgacagg cggcccattt gtctggaatg cgacgtataa aaatagtgta
    20101 cggttgtcta ctaatacctt gcttctagga atgatgggag ctggtaaatc gaccgttctt
    20161 aaaatgatcg cggaagccca tttagctgca ggagacttta tttggggctt tgaaaaggga
    20221 aaagacttta ttcctttctt aaaggaatat aacggtatta tggtgcgttt agacggatct
    20281 gacggaatga tcaatccttt agagatattt gccacacgga cgtatgatga agcatccagt
    20341 ttatatgacg atggatcagt caaagatttg aagatcaatg aagcagcgtc ctatcaaaca
    20401 cacttagaca aagtggtcta tcaggtacag ttagtgagtc ctcagctaaa aggaacgatg
    20461 aaagcagaat ttaagaccta cttaaatcgt ttttatgaag agtatggaac agtaccacgc
    20521 ggctttactt cctcaaattc gcgttcaaat actgatacgc aagtcacagg gaaagatcca
    20581 gaagcttatc cgacgttcaa agaattttta gactttttag gacaactgga attaccaggt
    20641 gcttcccaag agaaaaagaa tcgaaaagaa gaaatggaat ccatcgtaga atccttatgt
    20701 gagacgtatg gcatgatttt tgatggtcat agtacgattc gacaccttga ccaacaacag
    20761 ttggtctttt ttgacattga ctctattaca ggcttagatc gaggggtaca acaatgtcaa
    20821 atgtacatgg ctatgacgtt gatttggaat caggcattga ttcaaggaag aagacaacga
    20881 cgtttaatta aagaaaaaca aatggaagaa caagacttca aacgcttctt ggcgatgatt
    20941 gatgagtgcc ataatggtgc gacctgttct gcagtgaaaa gctgtgggtt gcaagtagtc
    21001 taaaaactat tgtaataact cttataacga gaagtcaaat gagtgggtaa cgccacaaag
    21061 ggcttccact gatactccga catgcaacaa gtagtttatg cagaccaacg ttgtgtgaag
    21121 ctcggtgaag tcggctgaag gataccgtaa ggtaaaagaa atagtgaata ccacgaaagt
    21181 tctctagagg taaagggtgt atccgataag tcgggtgtcc aaaaaaactt aatatggtga
    21241 gtatgtacaa acgtactgac gaactagcga atgtactgtc cgaaaggcat agctcctaga
    21301 aatgggagag acagtgagca aaaaatcact cattgtgcta acttgtggtt gagtaaaagt
    21361 cgttgttatg aaagaccatg ctgcgttaca ggcgtaggag aagttagggg gcaaaagcta
    21421 gcatctaagt taaggcatgt ggatagatat aagggaacag gggaagccat caacatggag
    21481 agtgcatact cgatgaagtg gtagtaagaa atatgtgaat gacaaataac ttactttagt
    21541 gatggtgaaa gtagagtcac gactgatgaa ggttctgtaa tggaactgga ggaacaggct
    21601 cgattcgtct gtaaaagtca tgaaacccgt tcaaccatgg gattctcgta tgactaaaaa
    21661 ggtcacgagc caattggagc gtgataaacc atgacaaaag aaacaagtcc aaaagatagg
    21721 aagttgcggc atgcagaata ttatggcatg acggaaatct tcgataagat atactccaaa
    21781 agtttgaagg gagataattt caagaatcta atgcagatta tcatttctga taacaacatt
    21841 gtcctagcat atcgtaatat caagagaaat cacggtagtg tgacaagagg agaagatgga
    21901 gtaacgatta aagatattga aaatctcaac cgtaatgaat tcgtagaaat tgttaggaag
    21961 cgattcgcac attataaccc tcggaaagta aggcgtgtag aaattcctaa atataatggt
    22021 aaattaagac cgttgggtat tccatcaata tgggatagaa tcgcgcagca atgcatctta
    22081 caagtattag aaccaatatg tgaagcaaaa ttcaataatc acagctatgg tttccgccca
    22141 aatagatcag cagaacacgc aatagcagat tgtatggccc gaatgaatca aagtcacatg
    22201 gaatatgtgg tagatgtaga tatcaaaggt ttcttcgacg aagtcaatca tactaaactc
    22261 atgagacaaa tctggtctat gggtatacga gacaaacagc ttctcttaat cattaggaaa
    22321 atgttaagag cacccgtagt acttccaaat gggaagttga tatacccaac aaaaggaaca
    22381 ccacaaggcg gaatacttag cccattactt gcgaacatca atctcaacga atttgactgg
    22441 tggatagcaa atcagtggga ggaaaaagat gccaaagagc taaaacatca ttttaggaaa
    22501 aatggagctt gggataaagg taatatctat cacaagctca gaaagagtac aactctaaaa
    22561 gaaatatacc tagtgagata cgcagatgat tttaaaattt tctgtagaaa ttataatgaa
    22621 gcgaaaaaag ttttctatgc aactaaaatg tggttacagg agcgtctaaa gttaccaata
    22681 tctgaagaaa agtcaaaaat cacaaatctt tcaaaagaag aaagtgaatt tttaggcttt
    22741 acgatgaaat tagtgaccaa aggtaacaaa aaggttgtta atacgcatat aggtacaaaa
    22801 gcacaaagca tgattaaagt gaaactaaaa aaacaaatca aacgaattca aaaaagtcct
    22861 aatagcaaag atacctttat ctatattgga gtctataaca gtatggttat tgggattcat
    22921 aattacttca aaattgcgac tcattgtagt gtagcaatgc gaaaaattgc aagagaactc
    22981 agaccaagtt ttgaaatacg actacgagaa caaggaatct caaaagaagg acaatacgga
    23041 aagtacgaag gatacaagcc ttacctacgg tcaggacaaa tacgatatat aagagggttt
    23101 ccaatattac cagtagggta tattcaacac agacatccga tgtgtaaaaa aagaaatatt
    23161 aataaataca ccgaagaagg tagggaagcc attcataaga agcaacaaac cgtagaagaa
    23221 tggaagatat cgtggttgtg tagcaatcca gtgcttagta agagagcaac gattgaattt
    23281 gcggacaatc gaatttcaaa attcatcgct caaaagggaa gatgtgcagt aacaggtgag
    23341 gagcttatct tatctgaaat gcattgtcat catattatcc cttatcatga aagcaaaagt
    23401 gatagctatg aaaacctagt gattgttact gaggaggttc atcgggtaat acacgctaca
    23461 caaagtgaaa cgattgagga acttctaaaa tatctaaaat taaatccaaa acaaaaagaa
    23521 aaactcaatg agttgagatt aaaagttggt aacgaagaaa tatcttaata aatatttgta
    23581 ggacgggaaa acagaatgtc ttttatagac gagggaacgc cgtatgcggt gaaagtcgca
    23641 cgtacggtgt ggactggggg aaaagacgga gataatatca aactcttacc tatcagtatc
    23701 gattaatcca gattttattg aaacggtaaa ttatgtcaca aactttcaac gagaaatgcg
    23761 taaagtagaa gcaatgactg ttttagctac acaatcccca caagaaatgg tgccagaaaa
    23821 tatgtcttct gataacttct cgaaactgaa gaaagtcttt gaattttctt ctacgaagat
    23881 ctttatgcgt atggatgaat ctattttacg tcatatagaa ggattagtgg gtaaatcaat
    23941 gacgaactca gaattagaat ccattcctca acaaaatcaa ggagacgctg tgatcaattt
    24001 tggctcaaaa gaaaccattc gtgttcattt tagtccgaac aaacgtcaat taaaattgtt
    24061 tgatggagga aaataatgga aaaccaaaat gaaagtttaa tcaagcagta tgttaagcgt
    24121 cgtgcaaagc ggcgcttatt tttgtggttg tttggtactt ctgcaggttt gattactatt
    24181 ttgatcactg tgttcgtgac cctatttcta atacttgcgg cgggctcgat tgataattcg
    24241 gattcagatt caagcagtgg tggcgaagct tttacaggag aatattcgga aggcttaccg
    24301 atttataaag aaatcaaagg aagaggccct ttttctgatg agattgcgca atatgccgta
    24361 ggtgcagcag tgaaatataa attattacca agtgtgatcc tttcgcaata cggttacgaa
    24421 tcagcgtttg gtacatcggc gtccgcaaga aatgatctca actattttgg gattacttgg
    24481 tttgatggtt gtctgtttcc taaaggaact gcacgaggaa ttggtggcat tgaaggtggc
    24541 tggtacatga agtttcccaa ctcaaaagct gcattttcct attacggctt tatggtagcc
    24601 acacaatcca attttaatgc ttgtgtagga aataaaagcc ctggcgcaag cttattgatc
    24661 ttaggacgag gcggttatgc cgctgcagga attacggaag atagtccgta ttatacaggt
    24721 tgtatgtcaa ttatcacttc taataaatta accgaatatg atgaattcgc catcaaacat
    24781 tggggtgaag gtggcaataa caacggcacg attactggtg aatggacgaa tccattccct
    24841 ggcagcagtt tagataaaag ttccttcagc ggtggtcaat tatttggaac gaatccaggc
    24901 ggtgaattcc gtccgaacgg cttccatgat ggattagatt ttggttcagt tgatcatcca
    24961 ggaagtgaga ttcatgcggt tcatggcgga aaagtcgtgt acgttggcaa tcctggtatt
    25021 tcaggtttgg gcgcgtgtgt gatcgtgatt aattacgatg gcttgaatat ggtgtatcaa
    25081 gaatttgcaa atagtacagg aaattctcga gtgaaagtag gcgatcaagt gaaagttggt
    25141 caagtaattg ggatacgaga tacagcacac ttgcatttag gatttactcg aatggattgg
    25201 cgtcaagcac aaggacatgc attcattgat gatggtacat ggatcgatcc gttaccattt
    25261 ttgaatagca gtaaaaaata gatcaggaga aaaaatatgg aaaataaaac gttaaaaatc
    25321 gtcttaacag ctgcaattag cgttagttta atcggcaata ttgcgttgtt ttggcagctg
    25381 aaagaggaac aagcagcaaa tacctataaa attgaagaag tggctaaaaa cgcgtctgag
    25441 aagctcaaaa aaaattcctc tgaggcacag gcgaagatcg acaagttaaa tcaaaccatc
    25501 aatgagaaaa accaagaaat cgatcagctg aagcaacaag atggcacaaa agcaaaagag
    25561 gaagtaactg aagcacaaag aaagacggca gaagagttcg gtcaattggc cattgataaa
    25621 tcattaagca gaaatgaatt aacggaaaaa ctaaaagatg tcgcaaccca agaagtgatc
    25681 gacaaattaa gtcctacaga cgatgatcac caacatgatg attacggaag ttactccttt
    25741 acattaggtg acgtgcaaac ttacgctcaa acaagcagtg taaaagagga acaaaatttt
    25801 gtcgtatttg ttgattatac aatcggcaat ccgcaattca aagatgtcaa accacaaaaa
    25861 ataaaaggtg gattaactgt cactgaaaaa caagtagatg gtcaatggaa agtcatagac
    25921 tttagttatt tcactcgtta ggaggaagaa aatgagtaag aaaaaaggag aaagacaacg
    25981 tttcaaaaaa cgaaaaagtt atcttagatg gctgggaatc tttatgattc tcggcttttt
    26041 gattcttgct agtccgattt atacgaatca gaaggtagat ataccagatg aggtaagcac
    26101 gaacgccttt ttaaacacga aagagctgac aatggtttca aaagaaaaag gagcgaacca
    26161 gcaattagtt tttgaatttg cgatcgatga tgaagatcaa agcttgttaa aagaactggc
    26221 taatcttaat tatcgcgtag aagtaaagac agctaaaggg gattatcaag caatacgagc
    26281 caaagtgatt aaggtgtctg atgattattt tgcagtgaag ctaacgcatg taccagaaga
    26341 atatatcgcc atgcgtttaa ccattattcc tgaaaaaatt gatccaaaag tggatatgca
    26401 agaaccacaa gatctgattt attacgtgca cgaagataag gttaaagatc aagtgaaaga
    26461 tgaagattat gaacaacacg cgatcaatta caaagtgaaa ggatacaaga aagaacaaaa
    26521 agcagctcaa aaacaaatgg aatctttcca agcaaccatt gatttgaatg aagaattagt
    26581 gaagaagcta aaagatcaat tgccttatca agtcgcagat gatcaggaaa atactgaaaa
    26641 caagattaat agctaccagc aagaaattga aacatcgaaa acgcaactga aagatcaaga
    26701 agagatcatt caaaaacttc aagaaaaaat tgatcgtatg acaaaagaaa agatttagaa
    26761 ggacaggtgg agtgaaaaaa tgaagcgaac aaacttggct gacaaagata ctttcgctga
    26821 gcagaaagta ttgattaaag cctatctcaa agttgtaaaa gctaaagaaa tatgtcgaga
    26881 agtgaattta tctaacacaa caatatctga gttgaagaat ggtcgaaaga atttagatac
    26941 tttaaaattc agatcatttg aagctctata tgtagctgct aaaaaatatt ttacagctga
    27001 taacaaataa aaaacaggag tattttatgt gaaaaaagta tataaaaaat gcttatgaat
    27061 cagctatatc accttaacca tttgttaatg agatgggaga aaacattaac cacgattcta
    27121 acgcaaacat caagcacata aaacatttaa cactcctcct ttctggctga agatttcaac
    27181 caaacaggaa gacagcgtat caattatagc atacactttt tcttaaattc atttttcata
    27241 tgacaaaata tttccttcta atcgacaaag ttagaaggat tttttattgt ctaaatacac
    27301 aaataacctt aatttttaaa gagcataagg aaacgtgata tcactttctc acgaaaggtg
    27361 ttaggtaggc tgtgcctacc acgtttctaa gaaagataaa tgatatcaaa attttggatt
    27421 tgtgtgtaga tttgtgggta ggatgaaagc gaatgtgggc agtaaaataa gcgtttgtgt
    27481 gtagcaaaat caaaaaatgt gtagggatat gtgtagctac acatatccct acacataata
    27541 aatttggagg aaaatatgga cttattaatt agaaatattg aagaaaagta tattaataaa
    27601 attgacaaac gttgtgaaga acttacggtc aagactggca aaaaatggag tcgaaatcaa
    27661 tacctaaaag ttttgatcga aaatgatttt gatcacgctc ttctgaatta taaaaaagac
    27721 caatttgatc ggttgttaga aaaatttgtg gatattcaaa cttataatac gaaaacttta
    27781 gaagaatata ttgcaacaaa taatcaatta attggtttgt taattgaata ataaaaatag
    27841 tattgtgaat ttataaaagg aggaaatcaa ttttggcaaa agcaacaaga aaacatgtat
    27901 atctttacga tatgcatatc gaagtacttg atgaaataat ttctactcat gaagatatat
    27961 ctaattatag tgaagcagtc agacatttgt gtatgatgta tgatccaaaa atcgaagaaa
    28021 aaataaatcc aattcaaatt aaattgaatg caatgggaaa agaaatttct attttaaccg
    28081 aaatggtttc agagtttgcg gatcttcatt tgaaagatac aggaatacta attggtacaa
    28141 attcaactgt ttatcaagaa gcagaagaac gtgtggaagg aaaaattaaa tccaatcaaa
    28201 ccaaaaaata ttcttctaag ttaaagaaac cagtgaatga atctgttcat accagagtag
    28261 atccatttga tccaacgaag attctaaaaa gataaaagta gaaggagaac gtgatgaata
    28321 aatcgcctgc agttattctt acttgtcaat ttaccttgcc caataaaaaa tctttttcaa
    28381 catacattga ttatatgaca agagaaaaag cactggaaga aatggaacga cgtacgcccg
    28441 aagaagaaca ggaactgcaa cgaatcaaga atgctttaga ggatttttat attcctgaag
    28501 gagaaacttt ttcggaaagt aagacggata aagaattcag tgaaacgtcg aatgaagctc
    28561 aatcgttgat ggaacatgga atcaattttg acaagttaga ggaacaagat tttaccaaat
    28621 atctttctta tatgacccgt cactatgcgt tagaacaaaa gaaagaactg acgcaaaggg
    28681 aacaaaaaga ataccaacgg ttgaaccaag caatgaaaaa atatcaaaca gagccacata
    28741 aaaaagcttc aggaaatctt cctggagttt tttctatggc ttcagacgaa gtgaaaggaa
    28801 gtgatctgaa ggagatcaaa caaatcttcc aaagaggaca acaaaaaggc tcaatcattt
    28861 atcaagatgt tatctctcac gacaattcgt atcttgaaaa aatgggcttg tacgatccca
    28921 aaacagatac cttagatgaa gaaggtttga aagcagctgg aagaaaaatg atggagacat
    28981 tattcgagaa agaacaattg aatgaaacag gttactggat ggcaacgatt caccgtaaca
    29041 ctaagcatat ccatattcat tttgcagttg ttgaaaaaga aaatacgcgt aaaccgtata
    29101 ctgagattga aaatggtgtg agttatatcg taccgaaggg gaagagaaga caagcaacat
    29161 tagacgcaat gaaaaccaat tatgtacatg agttagaacg tttctcttat gaaaaggatc
    29221 gaatgatcgg tatggagaaa cgaaatttat tgacacgaaa aagtgatttg cgtaacacct
    29281 taacaaaagt agtaaaagaa ccagcacgtt acgatcgaca cgccatgaac ttattaaatg
    29341 aagtatataa aaccctacct gaaaaacgtt cggaatggaa ttatggagat gaaaatcgaa
    29401 ccaagctttc accatttaca aggggaaaac ttgatgagtt aaccaaatat gttttaacaa
    29461 aagaacctgg atatcaagaa tatatacaga tttcaaaagc attgaaagaa gaagccaaaa
    29521 gcctttatgg ggaaagcaaa agagaaacaa agaatgcaga aaaaaacgcc ctctttgatt
    29581 taaaaaagcg cacaggaaat gcgattttaa cagaactgaa gagacaggat agtcaaataa
    29641 aaccattaat agaaagtgtc aatcagatgc aatctattta caatgaacga tcctttcgaa
    29701 cactcaaaca gttgccaaca aatgaataca agtatagtta tcaagaattc gtaaagcgta
    29761 ggaaacaatt ccaacaaaga gttgtcacgc agcgaagctt gcgtcaatta aatcgaacaa
    29821 ttaatgcaca tgaagaaaaa taccacgcaa gtaaagcata tgaagagcaa cagcgaagaa
    29881 tccaaatgga acaagaaaga caataggagg aagagaaatg aaacaatatt tagatcaatg
    29941 gaaagtcata gaaggatcac taaaagaaga acgaatcgaa caacttcctg attgtttaga
    30001 aaaagagcat ttgtttcaga taagcgaaat gttgagaaac gaacagtttg atccaaatca
    30061 atttttagtt gttgagtacc cagctactgg tgtgtattgt tgtaatcatg tgaagggtga
    30121 aaaatatttt attattcaag aatacgaagg taagttagcg ccttattata ctacttggga
    30181 aataaatgaa gagggaatca ataatttccc atgtgaaagc attgaagagt ctatttctct
    30241 cacggaatgt taaataaata gagacgtaaa agagtcctga acgtgattgt ccaggactct
    30301 ttttcaatga attgttttcc gccaattcac tgagtggtgc gattcaattt tattatatca
    30361 aaagaaagtg agattccaaa gtgaagggag gaagaagaaa tgactcagac aacgattggt
    30421 gttgtggttg aaaaaagcag ggataatctc gtttttgtga cagaaattca aacaggtaga
    30481 gcttttgtag tcacagacaa agctgcaaaa gcgtatcaaa atggcaatat tttaacgtta
    30541 aatctgacta cgaagatgtt cgttgatgca gtagaaaatt atccatttgt ttagacaaac
    30601 gatattttac aaggaggaaa cggacatggc agaagaaaaa gaaccactag ataatagtcg
    30661 gttgggaaag tccaaaagaa aattagtacg gttgcaaaac gaattaaata atcaatttca
    30721 aaagctattt gatcaccaaa aattgacaaa tgggcaacca atgaatgaca aacgaaatgg
    30781 tcgttcatgg tttaaccagc aagaaaaaat agaaaataaa atacaaagtt tgagagaaga
    30841 gatcaagcaa caagaaaaac aggtagaaaa acttgaaaga caagaagagc taaaggaaat
    30901 gggctataac aagtacggcg gactggatat gacgatcgat aatattccaa gaatcaaaga
    30961 agaaatcgaa cgctttgaaa aaggtgaatc gactttttca gccgccacga tccgaaaata
    31021 tcaacggaag ttggaaacat tagaacagct gaaagaaaaa tcagaaaaag gaaaagaaaa
    31081 tatcctccct gaagtacaag cgatcattga cagcggacga gtgacgcaat ggaaaaaaaa
    31141 tccaaccatt tattttttga aaggctatcg aaaagttgct ttagaactga atgaaaacgg
    31201 gggatttgag gaatcaaaaa aataccaagc cacgtcggaa gaggaaaaag cagctgttcg
    31261 tgaattactt gaagaggctg caccaactga ttctctgaag gagaaagaaa aagatcattt
    31321 ctatcgagta gaattcaacg aaaatgatcc agaaaaagga ttgaaaagtt atggtgggga
    31381 gatcgtaaca ttagaattga tcgatcaact taaaacgttg gataaccaat acgctgcaga
    31441 agcaggttat tataaattct attttgaaga agtgaagaac ggagaagtag tagatcagat
    31501 ccgaatggat ctaggggatg gcgtcaacag taatcacccc atctatttag agttagcaaa
    31561 attgtgcgaa caagtacctg agaaggaaca aaaacaatcg gtcaaaaatg aacctgtttc
    31621 gaaagaacaa atgcagtcga aagaaacaaa aacaaaacaa aagggctatc aaaaacgaac
    31681 ggcagaagag atcaaacaag aagttaaatc cctttcgcaa caggcactgg atgcggtgaa
    31741 aaagcatacc cattcttctg aagacgtcaa agaacttttg aactttatgt ctcgttttcc
    31801 agaacgatcc tttagaaatc aattattgat tgaggaacaa tttccaggtg caacggcttg
    31861 tttaggtcgt gccgcgttga aaaaagaagg aatctatatc aaaaaagggg aacaagggaa
    31921 caagatcttt gtcagaaaaa cggtcaaagg attttatgag aaaggacgtg gctttgttcg
    31981 agaaacagaa gctacaccag aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat
    32041 caaaaaacct tactacacga ttgaaaaagt gtttgatatc acacaaacac agttaaaacc
    32101 tgaagactat ccgaaaatct ttccgaaccg tgtctttgat tttcaattgg ataaaaaagg
    32161 acagtcagaa cttcaagctg ggattcaagc ggtagccaac aatattggaa tacaaattcg
    32221 agatatgaca gaaagtgaag tctatcaaag agaattagga caagcacgtg gggcgtatgt
    32281 tcgtaacgaa tttactggcg aagaagaaat cgtcatgaat actcgaaata ctcctacgca
    32341 gcatttagct acaagtattc atgaattagc ccatgcaaga atgcacaaat tttcagaatt
    32401 agataccgca actaaagaat tgcaggcaga aatgacttct tatattgtgt gcaaacattt
    32461 tggaatggac acgtcggaga aagctattcc ttatattgca gcatggacga agaatggtca
    32521 gacgttggat gataaggaag cggcggaacg tgggaaaatc atgaatgatg tcagccgtgt
    32581 cgcaaatgaa ttcattcaaa ccatttctaa tgaaatcaat caatacagag aaagagaacc
    32641 agagatccaa ccagcagaag tgaagcagga gcataaaaat actgtagaaa cggcagagaa
    32701 taagaatatt gtgcccgttg gttcactttg gatcgacaag aaagacggga aagtaatgga
    32761 agaagatacg ggtcgaacgt tacatttgaa ggatgcggcg tttattgaag atccgcagca
    32821 taatcaagtg attttaaaag gaaaaagtta tggaaaagag attcaaacgg cgttaccttc
    32881 agaagaattt gcgctaggaa atccattccg attttcactt gaaacagtgt cagaagtggc
    32941 caaacgaaaa aatgaaatct attatgaaca acaattgtca caaaaagaac gcgtataaaa
    33001 caagtaaaaa agactatctg aaaaacggat agtctttttt cttttgggag gtttgaaaat
    33061 gaccgaaaaa aaaatgagtc ttattgatcg atgtaaacag atcgatatag tggattttgc
    33121 tcgaaacaat ggaatggctg tagtcaataa aggacgagac tatcgcttag aagaccatga
    33181 ttcatttgtt tttgatcgaa gaaaacaacg tttttattgg aacagtcaaa atattagtgg
    33241 cgacattatt gaattagcta aacttttttt catagacaaa gagatccaag actctaagca
    33301 acaatttaaa gcagccactg attttattct aaagaatgaa gacaaaacag aacgagtgga
    33361 aaatcttcat tttgaaacag aaaagtacaa agatcacccc gttgattatc aaccactcac
    33421 agaaaaagga aggaattatc tgaaggaaga acgaaaacta ccagagtggc tgattgatta
    33481 tgcagaaaaa gaagggttga ttgccgaact aaagccaaaa tatgaacggc aaaatttctt
    33541 agttcgagac gatcgtttag atcatgcggt cgccttttta tggaaagatc cacagacaaa
    33601 agagacagta ggggcgagtt atcaaggaac gttcattgat tatgaacgtt ttggtgagag
    33661 aggcacatat aagcatatag ataaaaattc gacagcgaat catggcttta acctaaagat
    33721 cggtgaccct aagcagttaa aattttttga gagtagtatt gatttactta gctatgctgc
    33781 actgaatcgt gatcagttaa acgatacgtg gttagtctct atggaagggt taaaacacca
    33841 tgtgatcagt cattattttg gtgaggcagt atctgaattg agaaagaaac aagcctttcc
    33901 tcaatccatc gagatttgtg tggataatga ccgtgcagga catattttct acgaaaaaga
    33961 acaattaatg ggcgcagtcg atccatttac gaatcagaaa gtacgttgtg aacgaggaat
    34021 cgctaatgat tggcaagtgc caaaagaata caaaatcatt tatgaagaag tcgcaaaaga
    34081 agaaaaggtc acaccagaag ctattatggc catacacaag acagaaaaca atctgcaatt
    34141 aacgaaccaa ctggtttccg ctcacaaagt aaatgcgttt tttggccagc agctttctgt
    34201 taacgattca atagaagcca ttaatttgaa agatatttgt agagaagtcg caaaagaatt
    34261 aaaagtttgt gaacgcgtgg atggtacgta cgattttgat cgtttttatc aagaaaaagg
    34321 ggatattaat gcgcagattc ttttctctta taaagcagaa caatactaca aaggctataa
    34381 aaatcatgaa catgaattta tacctgaagt aaaaaaggac tggaacgatc aattgaagca
    34441 tgagatccag caacaagaaa tcagaaagca aaaacgtgcc atgttgtttc aacaaggcag
    34501 acaacaagaa agggagtgaa atcgtcatga tgaaaaccgt tattttagca gaaaagccgt
    34561 cacaagcgaa agcttatgcc gatagcttct ctaaagccac tcgaaaagac gggtattttg
    34621 aaatccaaga tcgattgttt cctggtgaaa ctgtgatcac gtatggtttt ggtcatttag
    34681 tcgaattaga ttcgcccgat atgtacgatg aaaattggaa acaatggtca ttagaacatt
    34741 tgcctatttt ccctacacat tatcactatc atgttcctaa agataagaaa aagcaattca
    34801 aggtggtgaa acagcaactt caatcggccg acaccattat tattgcgaca gatagcgatc
    34861 gtgaagggga actgatcgct tggacgatca ttcaacaagt aggcgccgat caaggaaaaa
    34921 cctttaaaag actttggatc aactccttag aaaaagaagc catttatcag ggattccagc
    34981 agttacgaga cgcaaaagag acctatccaa aatttgaaga agcacaagca cgtcagatcg
    35041 ccgattggtt aattgggatg aacggtagcc cactgtatag tttactcttg cagcaaaaag
    35101 gcataccagg aagcttttcc ttaggcagag tccaaacccc aacgctttac atgatttatc
    35161 aattgcagga aaagattaga aatttcaaga aagaacctta ttttgaagga aaatccgaag
    35221 taatcgctca aaatggcgca tttgatgcga agcttgatcc gaatgaaacc caagcgacgc
    35281 aagaagcatt tgaagattat ttaaaagaaa agggggttca attgggaaaa cagccaggca
    35341 tgattcgcca agttgaaacc gagaaaaaaa gcgcggcaag tccacgtctt ttttctctat
    35401 ccagccttca gtctaaaatg aatcagctca tgaaagctag tgccaaagac actttggaag
    35461 ccatgcaagg cttgtatgaa gggaaatatc taagctatcc acgaacagac accccttata
    35521 ttacggaagg ggagtatgcg tatttgttgg atcatttaga tgaatacaag cattttttaa
    35581 aggccgaatc aatcccaacc cctattcata cgccgaatag tcggtatgtg aataacaaga
    35641 aagtccagga acattacgcg atcattccta caaaaacggt tatgacagca gcggcttttg
    35701 aacaactaag tccactgcag caggccattt atgaacaagt gttaaaaaca acggtggcca
    35761 tgtttgcgga aaaatatacc tatgaagaaa caacgatctt gacgcaagtc cagcagcttc
    35821 agttaaaggc aatcggaaaa gtaccgatag atctaggttg gaagaagtta tttggcaaag
    35881 agaacgacgg aaaagagaag gaagaagagc cgctacttcc taaagtgaca aaaggagaaa
    35941 tggtgacggt agatctacaa gtccttgaaa aagaaaccaa gccaccacag ccttataccg
    36001 aaggtacgtt gatcacggcg atgaaaacag ctgggaaaac cgttgatagt gaagaagcgc
    36061 aatccatatt gaaagaagtg gaaggaatcg gtacagaagc gacacgggcg aatattattg
    36121 aaaccttgaa acaaaaggaa tatatcaagg tagagaaaaa taagctcgtg gtgacgaata
    36181 aagggatttt gctttgccaa gccgtggaaa aagagccgct gcttacgagt gccgaaatga
    36241 ccgccaaatg ggaaagctac ttgttaaaga tcggtgaacg aaaaggaaca caaaccactt
    36301 ttctagccaa tatccaaaaa tttgtctctc acttattgga agtcgtacca gggcaaatcc
    36361 aatccactga ttttggatcg acgttacaag aagtgaaagc agccagtgaa aagcaagaag
    36421 ccgcgcgtca tttaggaatt tgtccaaaat gccaggaaca agaagtctta ctctatcaaa
    36481 aggtggcagc atgtacgtct gaagcctgtg atttcaaact atggacaacg attgccaaaa
    36541 agaaacttac agcgacacag ctgaaagaga tcattcaaaa tagccgaaca agccaaccag
    36601 tgaaagggtt aaaaggtcag aagggttcgt ttgaagcaac gattgtttta aaagaagact
    36661 tcactacagg ctttgaattt tctgaaaaga aaaaaacgaa cttcaagaaa agaacacgac
    36721 gcactacaaa ataatggagg gttaatctta tgcgtaatac aacacacaaa caacaagaaa
    36781 tcgaaaatca aaaagccaaa attcgttcag aacaggaaaa aatgcgtgcc agcaaacgaa
    36841 gactgcagca attgaagaac cagcagcgga ttcaaaaaga aatcgaatta gatcgataaa
    36901 ttcctttctt gttaatataa aactaacatg ttatagttgt gttagtttta tattaacttt
    36961 aggggaagag taatgaaaga caaaatccaa tgtatttttg ataaacataa tggcaaccta
    37021 tccttgaagg atgctagaag tgaaggatta tcgcctatga cgttagatcg attagccgaa
    37081 aaggataaaa tagaaaaggt agcaccaggt ttctatacct taaaggatgc gttcgtggat
    37141 gaattgtatc tcgctcagag tatctattcc agagggattt tttctcatga gactgcgctc
    37201 gatctttttc aagtaggaac atatatccct aaaaagatca atatgatgtt tcctaaagga
    37261 tacaatgtat caaaggagaa attagatcag tatgccattc aaccgcatta cactaataaa
    37321 gaaaattttg acctgggagt cacagaaaca gaaagctttt acggcaataa aattttagtc
    37381 tatgatttag agcgaacgtt atgtgacatg tggaatccaa gatacaaagc aagcgtagag
    37441 gtaaaacaag aagcattaaa agaatatatg acaagtacca gtagaaatac aaataagcta
    37501 agaagataca tggatatttt aaaatcacca aaagaaatga ctttgtatat gttgccactt
    37561 tattgagagg agtaaagatg aatgacaccg acacaattta aagagaaaac aagaaatttc
    37621 gcagcagaga aacatttaaa cccacaaatt gtccaaagat attatatgat ggaaaaatta
    37681 ttagaatgta ttgttgaatc gccttatcat gatgattttg tattaaaagg gggatttctg
    37741 attggatcaa aatacgggtt agaaaaccgt tcaaccgttg atattgatac gacttatcga
    37801 aattcgaaac taacaaaaga aaagttagaa actgtttttt ctgaattaac agctaatcct
    37861 acaaaagatg gaattcgttt tgaattaaag ggattaacag agacgaggga agccgattat
    37921 tatccaggtt ttcaagcaaa gtttattgct tatcttgaaa atgcacgtgt accttttaaa
    37981 ttagatatta cttcaggtga ttctattatt ccagcagcat tgatgtatga acacaaatta
    38041 atgtttgaag ataaaacgat tcatattcca gcttatccta cggaacaaat tctagctgaa
    38101 aaattacatg ccacgtttag ttttggaaaa gataattcgc gcatgaaaga tttttatgac
    38161 ctgtatatga ttccgaaatt agaacgtatc gatgagaaaa atttgtataa gagtgttcaa
    38221 ggaacattca aggaaagaaa taatcaatcg gtgtttaagc atttgtatga tcaggaaatg
    38281 cctcaattgg tgaaagatga gggcttgaga gaaaagtgga aaagatacca acaagaaaac
    38341 tattttgcca aagacatttc tttcgaatct acactaactt ccgttgattc cctgatgcaa
    38401 tcaattgtag agcaagagaa ggcagaacga aaacaaaatt ttaagagatc acaacaaatg
    38461 aatcaagaaa tggagagata aagatgatca ataatgtaac tttagtaggg cgattaacta
    38521 aagatccaga tttacgttat accgcaagtg gcacagcagt ggcgactttt tccctagccg
    38581 ttaatcgtaa tttcacgaat caaaacggca atcgagaagc tgattttatc aattgcgtga
    38641 tttggcgcaa accagcagaa acgatggcga cattagctaa aaaaggcagc ttgatcggcg
    38701 ttgtcggacg aattcaaaca cggacttatg acaaccagca aggacaacgt gtgtatgtga
    38761 cagaagttgt ggccgataac ttccaactat tagaaagtaa agcagcgaca gaaagccgta
    38821 tgcacgctga tcaatcgtcc agcagtccaa gccctaccac ctttgaacaa cgggatacag
    38881 cgacgcctaa caacaatgga ttgaacgctt cacagaatcc atttggtgga caatcaatcg
    38941 atattagcga tgatgatcta ccgttctaag tagcattttc tattggttgc atttgctacg
    39001 gtttatgtta taatttagac aacaaaaaga attttcaatc tataaccaaa aaccactagc
    39061 tggcgggcta gtggctttct tttacgtttc tattataaga attaacgggt aggcttactg
    39121 tctttcttgg caaatttaca atcaagccat ttgcagatat agtgcgcggc tacttctgcc
    39181 atgattgcaa aaacaaaatc aaaaagaatt ttcaaaccta taacctcctc cccttgccag
    39241 attaggccgg ggtagcagac agcaagacta ttatagcata attgaaatat ccaaatgatt
    39301 ggatttttgc tataatttta gcgaggtgat tgtgatgaac aaatatgttg gtttgcttga
    39361 taaaatccga gtaataaaaa cgcaaccgtt attggttcgc tttactttac aaacgattca
    39421 tgaatcgatt aattgtgtag ttgcagatat tgaaattatc gataaattgc taattatgga
    39481 tgatggaaaa tacaatattg ccgtcacagg tcacttcaat aaacgaaatc agctagttat
    39541 tgctagcatg tatgttagaa atcccgatca ttttacgcga tcgatgggca tataaataaa
    39601 agaagagttg tagtcctaaa tgaggattgc agctcttttt gttttacata gaaacaggaa
    39661 aagacctttt taggaagaca caagtgcctt ctcaattgaa aaacctttct ctcactccaa
    39721 ccgccaccgc cgttcgagaa gaaaggaagt gaacgctgca cgttcactaa gatatggtgt
    39781 cttgcaaagc aagacaaaat agacaaaact tcctctcacc aaaaggttcg ttcctgttcg
    39841 ttatctcttt attgatttgc cagaccggca acaagttgcc ttttatcacc aaaaagcgac
    39901 aaggacaagg gtaaatggca cgctgcgcgc ccttgtcctt gtcgcttttc gttgatttat
    39961 tctggccaac aaatcaataa agaaagaact aacgaaagga agtaaaacct ttcctaagag
    40021 gaggcagaat caaacatgcg aaagaaattt gattattggg gtgttccctt cagcgaatta
    40081 ttcccaaact atcatgcacc acatactgtt gaatgcgatt gtggagaaag agcgaaatgt
    40141 atcaaaagct atcgtttgta tcaatgtcca acatgcggga aaaaatatac cttgtcttat
    40201 ggcgattaca ttttgattga tgagaaagga aagaaacgct gatgaaaaca caacacaaat
    40261 tattgactgt aaaagaagtc gttcaaattt tggtggacaa agaagtcatt tctgaaaaag
    40321 tagcagaagc tttacttatt ccgcggtact ggacgataga aaccccttta gtagaggatg
    40381 caccgactat gctatttgaa gacctagtga agattgcagg atatgaactg cattatcaag
    40441 aaaacatgga aacttatgaa atcgaatatt ggttaaccaa attagagaac taaaaaggag
    40501 aaaaaatatg gctcaaaatt actatgacga atttgtcaaa ttacctttgg ataaaatggc
    40561 acaaaaaatg gaggatatga cgttcctata ccatgaaaca cgtgtaccca aaaaacatta
    40621 caaggaaaaa ttgtcagtcg cagtggaaga aatgattgaa tcaggtgtcg aaatgaatct
    40681 cattgcgacg tactatcgaa cactggaaga actgaaaaaa caaaatggaa aatggttttt
    40741 ccaagcctta ctatgcttgg aagtaggtgt caaaccaagc actatcaaac cttccgaata
    40801 tcaagcctta gaactgactt atgcaaaatt cgcacagatt aagaaagcta aagcaatggc
    40861 tagcgactgg ctagatactt ttgaaagtat cgaaaaaaat ggtgcatttt atacgattga
    40921 atcggaggag aaagataatg aataaggtgg tttatcagct aacaacagaa gatgtttcgc
    40981 aatttttaac aagcaaagaa cacttgaatg aagtaatcag acggattcaa aaagacttta
    41041 gttgtatgga agaaatgaag tactttattc aaagagtgga agaacagttg tatatggaaa
    41101 gcgaaacaag tcaaacgaag gtcacagtca gccataatca tttagacgtg tataaacgtc
    41161 gtcctgaaac ggtaaaaaaa ggaacagagc ttctacgact agaaacagaa aaatacatca
    41221 tgaatgtgta cctagaaagg agtgagctgg tcgaaaactt tgtcagctac acgatttgta
    41281 ggaaacacta ttactcagaa tcagaatatt attcaatttt cgagagcatg gaattaatgg
    41341 aagaagaaat ggaagagcta aacagcaaaa atgcgacggt agacgattat taccttgtat
    41401 ttactaggtt tttgacgtgt gggacggaat ctatcgtgtt aatgggaatg gattatgaac
    41461 tcgctgtacc cgaagcattt ttgagggaat atgtagaaga agtagataac ccaggcgacg
    41521 actggtacac gactttaact gattttttag acacctacac ttacgacgat acagaagaaa
    41581 tccaaaatga acttgaccgt aaacgaatgc catatcaacg atttgaaagg aaaaaaggta
    41641 aataaccatt tataccgaaa agtgaggatg tgtgaaataa gaacaaaaaa ccttgaaatt
    41701 aagggaatgg ttcttatttc gcaccaatcg ctccaaccgc ccccgccgct taggtggtgc
    41761 gaaataagcg ggcaaagccc gataacctaa gagaaagaag gagatccata tgaataaaat
    41821 cattcctcta ttgttcgtac tgtttgttgg cgagatactt atgcgtcatg ctgcagctgt
    41881 acatttaaac ttcgtagtat tcttaggaat actggtattt tgtttgatct tttggcgatt
    41941 tgtattagcc cttgtgagtc tactattcaa gatgattgca gccatatttt tcatagggtt
    42001 atttcgatga aaggagagag tactaatgcc aattgcaata ggaaataaac ggttacccgt
    42061 gacattagat gaaaagagac aaaaagaatt gcagcaacta aagcagaagt acggaaaaag
    42121 tgaatctaag attatgtgta ttgcgttaga tctattgatc gcgcaagaaa aagcaggatt
    42181 tgatatacca gcactaaaaa agtgacgtca cttttcattt gaagaaacaa aagtgatagc
    42241 acttaattgt tcctactatt tagaatattt tctatgtcct ttttattcca ttttgtatca
    42301 ataactatga aatctttaaa gtatccagaa gagattattt ttagatcaat ttttttctta
    42361 cctgattcta aagattttgt tttcttaaac atattatgat ttcctttcga ctattttcat
    42421 ataaatgtta acatagttta tggattcatg gaatgttaat ctaatttaat agaaaaggat
    42481 aattaagtat atacgttttt tcttatgtat gttatactat gtatatacag aaagaggtgt
    42541 ctactatgga aatgttacct ataaaaaaat ggggaaatag taatggcctt agattgccaa
    42601 agtatattat ggaatatctt ggaattcata cagaagacaa agtgaagata atacaagaag
    42661 aagtaaacgg tcagaagcgg ctaattatcg aagcggctaa tatagaaaat gaattaacaa
    42721 ttgaacagtt atttgaaaat tacactgatg aaagaaatca tgtgacgatt caaaatttag
    42781 gtgaagcagt aggtaatgaa aaatggtgag aaaacctaga caaggcgata ttctattatt
    42841 gaatacagca cctcgatctg gacacgaaca gacaggtaaa agaccttata ttgttttaag
    42901 tcatgatatt attgcggatt atagtaacgt tgttacagtt gctccaattt ctagtacaac
    42961 tagaaactat ccgctatatg taaatataaa ctcagaatat gaaatgaaga caacaggaaa
    43021 agtattatta gatcaactaa cgacaattga ttatgaagcc agagagtgta tttttttgga
    43081 aaaagcgcat gatagtttag ttgaggagtt attgatgaaa gttagaacag tttttcaaaa
    43141 agtaagcaaa taaaatagaa acagcagcat gatcaactat tcgtgattga ccatgctgtc
    43201 tttttttatt ctatatacgg tttgtctcgt aacattgtag tcttttgcaa tcttagcgat
    43261 cgcaacacct ttttttagat cctcaacgat gtttttataa acaagacgac gttggggatc
    43321 ttttgcattg gcactgtata gcttaggacg tcctttataa acaccgttcg cttttgcaat
    43381 cttaatgcct tgtgcctgac gtcgtttaga ttcggtacgt tcttgttcag cgatcatggc
    43441 caaaatttga atgatcaaat ctttaatgaa tttatccagg agaggattgc caattgcttc
    43501 ggccatgatc ggtagacttg tgatgatcag ctgcacattt ttcttcttca agtaattgac
    43561 tgaagcgatg atctcgtcgt aatttcttcc aagtcgatca atagcttcca caataaaaat
    43621 atcttgatct cggacgaaat ccagtgcttc ttgaaaaaca agtcgatgag taatcgttgc
    43681 gccagattgt ttctcaatga aaattctctc tgcaccgaat tttttcattt cttcaatctg
    43741 ccgttgttca ttttgatcga tggatgagac acgtatgtat gcaactctca aaatttttac
    43801 ctactttctg caactattta aaaaacaccc taatcataca cgagaaaacc agatttttca
    43861 atgtcgcggt cgtgtctagt tagggtatac cttatataga caaaaactaa tgatggagga
    43921 aatagtatgt gtcacttttc tttttaaatg gcaatatttt ttgtaaataa atgtcaatca
    43981 agaaaattta aacgattatg acattaaaaa tatattttat gaattttcta gtatataact
    44041 tgatttatct gttaagattt attaagaaaa cgttttctta tttttgattt cgaggtgata
    44101 gacaatgaaa aataagttta ttttatcttt aggtatttta actattggaa ttagtgtaac
    44161 tggaactaca attcctacat ttgctgatag tacaaatgaa ccaactactg tagtagataa
    44221 taaccaagaa ttaactcctt tagataggaa cggaatagcc attggtaacc caactaacga
    44281 cttaactgtt actgaaaatt atttaatagt ttctccatta cttgctcgag cagcttctaa
    44341 aacgatacga gtgacaggaa cgatgatttt tgataaagtt ggttggcctt ctagacctcc
    44401 tcaatcacgg aattggaaag aaaaaagaaa tggttattgg tactatggaa aattgtattt
    44461 acaaagttat aaatcagctg gaaacaattg gattgcgact tattcaggaa ccttaactag
    44521 aaagtaggga ttttttatgc cagaatttgt gaacacacca aatttttatc aagatagcga
    44581 tattctaagt aaaattaatt taccgaaaga agaatcagct ttacaagttc agcgaacttt
    44641 acaggagaca caaaaaattc aaaaatcaat ttctaatcta gaagattctg aagaaaaaag
    44701 aaaaaaaaga gaccaaaaaa catatcaaaa agaaaaataa tgattttaaa aaaagagtgt
    44761 ctagtcgaca ttcttttttt ctaattgaaa gtgttcataa aatcacccat ttctggacac
    44821 tttctttttt tgctttattt ttcaaaaaaa caattcattt catgttcata aacccataac
    44881 aatatctatg ttcagttaaa ctatatggat aaagttatgt tacaataaaa ttaatgaagg
    44941 agtgattttt ttgaaaatag gatacgcccg ggtatctact ggtttacaaa atttggattt
    45001 acaagaagac agcctaaata aatttggttg tgaaaaaatt tttactgatc atatgagtgg
    45061 ggcaaaaagt aatcgaccag gattagaaat ggccattgat tttgttcgct ctggcgatac
    45121 acttgtcgtt tggcgcttag atcgtttagg tagaaatatg gaagatttga tttcaatcgt
    45181 caatcgactg aatgagcgtg gtgtgagttt tcatagtatt caagaaaata ttacgatgga
    45241 caaatcaagt tctacaggac agctcatgtt tcatttgttt gcggcatttg cagaatttga
    45301 gcgtaatttg atcctggaac gatccgcagc aggacgagca gctgctcgtg caagaggacg
    45361 gtttggtggt cgtcctgaaa aattgtctga acaagatttg gaattactga agactttagt
    45421 ggatagcggt acacctatta agactattgc ggaacggtgg aacgtctcaa gaaccaccat
    45481 ttatcgttat ttggcaaaaa tggaggagaa aaatggaaag acaaattctc attagttttt
    45541 gtcatttaat gctgaaaatt tttgccattt atctagaaaa gtgacaatag tatgagaatt
    45601 ttttgtaagt gtctactgat aacttattct caaatgttgt aaggatttta ttcatttgtt
    45661 aatatta
//
LOCUS       DACECR010000013        40269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.013, whole genome shotgun sequence.
ACCESSION   DACECR010000013 DACECR010000000
VERSION     DACECR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.013"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(403..1635)
                     /locus_tag="IWO17_000661"
     CDS             complement(403..1635)
                     /locus_tag="IWO17_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0289558.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1889..2458
                     /locus_tag="IWO17_000662"
     CDS             1889..2458
                     /locus_tag="IWO17_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0289559.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2636..3076)
                     /locus_tag="IWO17_000663"
     CDS             complement(2636..3076)
                     /locus_tag="IWO17_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ0289560.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3234..3998
                     /gene="map"
                     /locus_tag="IWO17_000664"
     CDS             3234..3998
                     /gene="map"
                     /locus_tag="IWO17_000664"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ0289561.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4030..4953
                     /locus_tag="IWO17_000665"
     CDS             4030..4953
                     /locus_tag="IWO17_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ0289562.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5029..6168
                     /locus_tag="IWO17_000666"
     CDS             5029..6168
                     /locus_tag="IWO17_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ0289563.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6161..6961
                     /locus_tag="IWO17_000667"
     CDS             6161..6961
                     /locus_tag="IWO17_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0289564.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6961..7788
                     /locus_tag="IWO17_000668"
     CDS             6961..7788
                     /locus_tag="IWO17_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0289565.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7766..8500
                     /locus_tag="IWO17_000669"
     CDS             7766..8500
                     /locus_tag="IWO17_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0289566.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8600..9466
                     /gene="rfbA"
                     /locus_tag="IWO17_000670"
     CDS             8600..9466
                     /gene="rfbA"
                     /locus_tag="IWO17_000670"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ0289567.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9480..10052
                     /gene="rfbC"
                     /locus_tag="IWO17_000671"
     CDS             9480..10052
                     /gene="rfbC"
                     /locus_tag="IWO17_000671"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ0289568.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10074..11102
                     /gene="rfbB"
                     /locus_tag="IWO17_000672"
     CDS             10074..11102
                     /gene="rfbB"
                     /locus_tag="IWO17_000672"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ0289569.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11200..12051
                     /gene="rfbD"
                     /locus_tag="IWO17_000673"
     CDS             11200..12051
                     /gene="rfbD"
                     /locus_tag="IWO17_000673"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ0289570.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12086..14119
                     /locus_tag="IWO17_000674"
     CDS             12086..14119
                     /locus_tag="IWO17_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289571.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14163..15443
                     /locus_tag="IWO17_000675"
     CDS             14163..15443
                     /locus_tag="IWO17_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0289572.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCRSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15653..16459
                     /locus_tag="IWO17_000676"
     CDS             15653..16459
                     /locus_tag="IWO17_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0289573.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16471..17691
                     /locus_tag="IWO17_000677"
     CDS             16471..17691
                     /locus_tag="IWO17_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0289574.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17681..19267
                     /locus_tag="IWO17_000678"
     CDS             17681..19267
                     /locus_tag="IWO17_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0289575.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19306..21444
                     /locus_tag="IWO17_000679"
     CDS             19306..21444
                     /locus_tag="IWO17_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0289576.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21812..22795)
                     /locus_tag="IWO17_000680"
     CDS             complement(21812..22795)
                     /locus_tag="IWO17_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAQ0289577.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            23173..24564
                     /locus_tag="IWO17_000681"
     CDS             23173..24564
                     /locus_tag="IWO17_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0289578.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24580..25620
                     /locus_tag="IWO17_000682"
     CDS             24580..25620
                     /locus_tag="IWO17_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0289579.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25639..26724
                     /locus_tag="IWO17_000683"
     CDS             25639..26724
                     /locus_tag="IWO17_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ0289580.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26757..27719
                     /locus_tag="IWO17_000684"
     CDS             26757..27719
                     /locus_tag="IWO17_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0289581.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            27712..29139
                     /locus_tag="IWO17_000685"
     CDS             27712..29139
                     /locus_tag="IWO17_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ0289582.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            29141..30211
                     /locus_tag="IWO17_000686"
     CDS             29141..30211
                     /locus_tag="IWO17_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289583.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            30198..31619
                     /locus_tag="IWO17_000687"
     CDS             30198..31619
                     /locus_tag="IWO17_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0289584.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            31632..32639
                     /locus_tag="IWO17_000688"
     CDS             31632..32639
                     /locus_tag="IWO17_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0289585.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            32651..33655
                     /gene="neuB"
                     /locus_tag="IWO17_000689"
     CDS             32651..33655
                     /gene="neuB"
                     /locus_tag="IWO17_000689"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAQ0289586.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            33652..34800
                     /gene="neuC"
                     /locus_tag="IWO17_000690"
     CDS             33652..34800
                     /gene="neuC"
                     /locus_tag="IWO17_000690"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ0289587.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            34773..35450
                     /locus_tag="IWO17_000691"
     CDS             34773..35450
                     /locus_tag="IWO17_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0289588.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            35440..36582
                     /locus_tag="IWO17_000692"
     CDS             35440..36582
                     /locus_tag="IWO17_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAQ0289589.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            36595..37572
                     /locus_tag="IWO17_000693"
     CDS             36595..37572
                     /locus_tag="IWO17_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0289590.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            37572..38480
                     /locus_tag="IWO17_000694"
     CDS             37572..38480
                     /locus_tag="IWO17_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0289591.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            38481..39233
                     /locus_tag="IWO17_000695"
     CDS             38481..39233
                     /locus_tag="IWO17_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAQ0289592.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            39238..40185
                     /locus_tag="IWO17_000696"
     CDS             39238..40185
                     /locus_tag="IWO17_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0289593.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT        13440 a         6135 c         7843 g        12851 t
ORIGIN      
        1 gtatgtctta gtccatcaat aaacagtcga agagaaaata gggaaaaatt gattactaaa
       61 gaaaaactta ctaggtcgac ggtgtagttg attttcatac ttcaatagat tcgaattaaa
      121 cgatttcgat tggcttatac cagtcctatc caacgaaaga tatatcgttc ttaactctag
      181 cggtataact tgaagcttta cgtaaagcca atgggaatga actggagatg actttttatt
      241 atttttagta atagctgatg ggttaatact tacaactttc cttttcaaag taccatataa
      301 aaaaacggtg gggttgttta gcgaaataaa ggatctaagc caatagctta gatcctttat
      361 tctatgcttt atattatttt ttgcatgtgt gcgaaagtat actcaagccc aatcgccatt
      421 tcggaaaatc ggtactgtag agccatcttc acggattccg tcgatatcca tcttgtcaga
      481 tcctaccatg aaatctacgt gggtttgact tctattcaat ccagcttctg ctaattcttc
      541 atcggacatt tctgtaccgc ctttcacact gaaggcataa gcagaaccta gtgctaagtg
      601 attcgatgcg ttttcatcaa ataatgtatt gaaaaagatg atgccagatt gagaaatagg
      661 tgaagggtca ggtacaagtg cgacttctcc taaacgacgc gcaccttcat ctgtatcaag
      721 cagttttgct aagacatctt ctccttgttc ggcagaaaaa tcaacaactt ttccatcttt
      781 aaaagtaaat ttcatacccg agatgatggt gccagcataa ctcagtggct ttgtacttga
      841 aatgtaacca tctactcgat gactgtcagg tgctgtgaac acttcttccg tcggcatatt
      901 tgccatgaat ttttctccac gagcattata gcttccagcg ccttcccaaa gatggttttt
      961 aggcaatccg ataatgatat ctgtaccggg agcagtatag tgaagggcag aaaattgttc
     1021 tcgattcaat tcttctgctt tttttgctaa tttttcatca tgcttcttcc atgctaagac
     1081 agggtcttct tcatagacac gagtcgtttt gaaaatctga tcccataatg cctcaacttg
     1141 tttttcttct ggcagttctg ggaagacttt tgctgcccat tgtttaccag ccgcagccac
     1201 gacagtccaa ctgactttat ttgcttgagt tgccttgcgt aaattcatta gtgctttgcc
     1261 attcgctgat tgataagacg ctactcgatg gctatctaca ccagcaaatg catcgggatc
     1321 ggcagaaaca acgctgatcc tgcttgctcc tttttcaagc cattcatccg cttgatcgat
     1381 tttagattgt ggtacattct caatacgatc cgttgcagca tgtaaaagaa attctctttg
     1441 gatctggtcg tcagtccatt gaacgataac ctcagcggct cctagtttat aagcttcttg
     1501 tgtgatcaat cgagcaaggg gtgcctgttc cacgctgatc tgtaaaacaa ctgtgtgtcc
     1561 tttttctgtg gctacgccag tttctgcaat taagcgtgca tattttttta gaagttcatt
     1621 aaaatcagat aacataataa aaattcctcc tcaccatttt ttctggtaac tattagcatg
     1681 ataccattta attatgattc aaacaatgag gctttaaata aaaaaaggag gttatttttt
     1741 taaggttaag tttcatgata tatgttacta cttgctattt cagcttgtga agaaaatcaa
     1801 aaactaattt tgcatatcaa aaaaacatgt tacaatgaaa ttaatcaatg gtggaagcgt
     1861 tataaaaaaa ttaaggagat taaaaaatat ggcaagaaaa aagacgatta ctagagaaca
     1921 gattttggca gctgcttatg aagtagctgc caccgatgga tttacacgat ttactgcgag
     1981 aaacatcgcg aacaaaatga attgttccac gcaaccgatt tatcttgagt ttaaaaatat
     2041 ggatgacttg agacaagctc tttttgagca aatctatgat catttacaat atgaagtgtt
     2101 cactgttgag catacaggga atacgattat cgatcttgct tttaattata tccattttgc
     2161 aagaagagag aagaagttgt atcgttcact gtacttagaa gaatatggtg gaggaaaaga
     2221 gatgcagaat ttctcttata cttactttgt gaatacagtg aaacgcgatc cagcttatgt
     2281 gaatctttca gaagagcaga tcgaatcttt gtataatggg acatggatca tagctactgg
     2341 gatcgcagct ttgatgtctt caagcatcat ccatccaact gatgaacaga ttgaaaagat
     2401 gatccaagac agtatagatg ctatcttgaa attcaaagaa ccaatcgaaa tcgattgaat
     2461 atactcaaaa gttccacaaa tcagtaggta acaagagatg tgtcaaaata aggaaaagtt
     2521 cgtttttctt acagcgtatg catttttaac tcccgaagaa ctcttccatg aatgcaatga
     2581 ataaaagagc catacagatt gaataaatct ggatggctct tttattgcaa gtatattaaa
     2641 tttttgcgac tagttcttta gcgaaagctt ctaaattttc gatatcttct tcttcagcat
     2701 tcagatccac tttaacattt tcggctcctt ttgatgcgcc tgtttttgca aaagcagcat
     2761 cgaaatcatc caccgatttg cagaattcat cgtaaaaagt atcaccagat ccgcagacac
     2821 cgtaaatttt tcccaataga tccaattctt gcaagtcttc ataaaagtct acgatctcat
     2881 caggcagatc gccatcatcg tatgtataag tagctactac gcaaatatct gcctcctcga
     2941 attcatctgc atctacttgg gtacattcgt tgatttcgac ttcgatatcc agattttcaa
     3001 atgcttctgc aacaatatcg gcgatttctt cagtgtttcc tgtcatgcta gcatatacga
     3061 tttttgctaa agccatgatg tgcctcctcg tttataatta tttaacaatt atgaattata
     3121 ccaaaacatc attgaaaaga aaataaaacc taaagacacc tggggaatgt catttggcta
     3181 aatgtatgcc gctatgttaa aatgagaggg atttgtaaaa aggagacgaa aatatgatta
     3241 ctttaaaatc agcacgtgaa attgaaatga tggcagaatc tggagcttta ttagcagatg
     3301 tacataaaaa tctgcgtgat tttataaaac caggaatcac aagttgggat attgaggtat
     3361 ttgtcagaaa ctatatcgag agtcatggcg gaattgctgc gcaaattggc tttgaagggt
     3421 atgaatatgc gacttgtatc agtataaatg atgaaatttg tcatggattc cctcgtaaaa
     3481 agcctttgaa aaacggtgat ttggtaaaag tcgatatgtg tatcgatttg aaaggcggca
     3541 tttctgattc ttgttgggcc tatgtagttg gagaatctac acctgagatc gaccgattga
     3601 tggaagtaac gaagaaagct ttgtacatcg gtattgagca agcacaagta gggaatcgga
     3661 ttggtgacat cggtcatgct atccaaactt atgtagaagg cgaaaatcta gcgatcgttc
     3721 gtgattttat tggtcatggc gttggtccta cgattcatga agaaccagca gttcctcatt
     3781 acggagaagc tggaaaaggg ttgcggttga aagaaggcat ggttatcacg atcgaaccta
     3841 tggttaatac cggaacttgg aaaatgaaaa tggatcctaa cggctggact gcgtatacaa
     3901 gagacggcgg actttcttgc caatatgaac atacattggc tattactaaa gatggtccaa
     3961 gaattttgac atcacaagga gaagaaggca cttattgatc tatccagtca ataggtacgg
     4021 gagaggtaca tgaaagtttg gaacaaaata aaacaaaatg aaaatctgat gcgctttatt
     4081 gagacgactc aaaagcgcat cactgattca gaaatgagta cgacctctgt cgtcgttgct
     4141 tattatttac tcttgtcttt atttccattg attattgctt ttggtaatat tttaccgttt
     4201 ctccacatcg atcaagagac tgtactgacc tatttacgag aagtgattcc tgagaccgtt
     4261 taccagttta tcggtcctgc aatcaaagac cttttgacac aaagttcagg aggcttgtta
     4321 tccgtttctg cattagcggc tttatggtct gcaagtcaaa gtatcaatgc tttgcaaatt
     4381 gcgatgaata aagcgtatgg cgtagaaaat agaaaaaatt ttttgatcgt ccgtttcttc
     4441 tcactaattg tcattgtctt gttcatgatt gctattagtg gggtaactat cgttcttggg
     4501 ttgggacagc tgattttgga tgctattcag ccaatcgtac aaatacccgt gaactttatc
     4561 gatcagttcc aaactttgaa gtggccaatc acattggtag ctttatttgt catcatgttc
     4621 ttgatttatt taattgtccc taatgctcag ttgaagttga agacgatcat tccgggcacg
     4681 atcttcgcta ctgtcggctg gatgctgtta tcacaagtgt ttagtatcta tgctaggtat
     4741 tttgcaacga ggatcagcgg ttatcagatc ataggaagtt ttattgtact catgctttgg
     4801 cttaattttg ctgctacgat catcattttg ggcggaatca tcaatgcagt tgttcaagaa
     4861 tacgtgacgg gtcatcaaat caaaaaacgt cgagatccaa gccgaaaatg gttcaataaa
     4921 ttgaaaacta aattttctca taaaaaaaag tagaaacaag agttcttatt gtaaatttat
     4981 atcagtaatt tataataatt atgactctta attaaagtag gtgcccatat gcttgtttct
     5041 tttagtatgg ttataagatt ttttctgaca attttattat cactgatatt aacacctgta
     5101 ttcaaaatca tttcagtaca aacaggtatg gtcgacaaac caaatgaaag aagaataaat
     5161 aaagttccaa tgccttcagc gggcggactg cctatcttta tttcatttgt tatatcgact
     5221 ttattcttat tccgtaaaat tatccctcaa tcgtatattg taccgatctt gattgcttct
     5281 ttagtaatca tagtcacagg agtgctcgat gataaatatg agctgacacc taaacaaaaa
     5341 agtataggga tcttgattgc ttctcttatt atttattttg tagcagatat acgtattgat
     5401 tccttcacgc ttccttttat tggatatatc caactaggct ggcttagttt tcccgtaacg
     5461 attttttgga tcttcggtat cacgaatgcg gtcaatctga ttgacggttt ggatgggctg
     5521 gcggcaggga tttcattgat cggtttaatt acgataggaa tcattggata ttttttcctc
     5581 catgcttcta cagtgtatat tccagttgtt attttctgtc ttgtagcaag tattatcggt
     5641 ttttttcctt acaattttta tccggcaaag atttatttag gcgatacagg agctctattc
     5701 ctagggttta tgatggccgt cctttcacta caagggctga agaacgtgac ttttgtttca
     5761 tcgatttctt tgctgatcgt catgggagtg ccggtaacag acacgttttt tgctatcatt
     5821 aggagaaaag caaaccgtgt ttctttttct acagcagata aaaaacatct tcatcaccga
     5881 ctaatcagtt taggctttac tcataaaggt gctgtcctga cgatttatgc gatggctttg
     5941 atgttttctt tcacagctat ggtcatgaat tatactggaa gaatcggaac gatcatattg
     6001 attattgcca tgttgtttgc tgcaatttta ttatttgaat tgattggtct gatcaatgag
     6061 aaacatcagt ttattttaaa aacattgcga tttttaggga ataaagagta ccgtacgcaa
     6121 gtaaaacaga aatatttgaa gaaatggaac cgtcatatag atgaataaca aattaaacca
     6181 gccgatcctt attagtattg tgacggcaaa cagtgataaa atttttcgga cactagataa
     6241 ttttatttca actgtaaaag atgaaacagc agtaaaaatc cgaatttttg acaacaattc
     6301 acttcccgag tacaaaaaaa gactaacaga gtataggaaa tatccctaca tcgatatcta
     6361 ttttcatgat gaaaattccg gatttggtta tggacataat cacaatctga tagctagcga
     6421 ttctcgctat ggcattattt gtaacccaga tattttagtg gatgaagaga cagttacagc
     6481 attggtcttg ttattgaaag aacatccgga atgtgcgatg ttagcaccta agattcttaa
     6541 tgaagatgga acaactcagc acctcatccg tgagcagctg gctgtttttg attacgtttt
     6601 gcgctttgtg cctttcaaat gggtcaaaaa aaagtttgac aaacgactgg ctgtgtttga
     6661 atgccgagga ttgcctactg atgctaatag ttatgtacgg atgatctcag gtagttttat
     6721 ggtagctgat attgataaat ttaaagagat aaaggggttt gatgagcgtt tcttcatgta
     6781 ctttgaagac aacgacttgt gtttgagttt tgaaaaagca ggttacaagt tattgtacac
     6841 acccttttat gcagttatcc atttttacgg gaagggtgcg catcgaaatg caaaactgtt
     6901 tcgcgtgttt ttgacttcta tgatgaaatt tttcaataag tgggggtggc gttttttctg
     6961 atgaaaccag aaatattagt agtcgttgtg ctttatcagc aactattcag tcaatctcca
     7021 gcttatggac cattaagcaa agcacttagc gaaaagcaaa ttcagcttat tatttacgat
     7081 aatagtcctc aaaggcagca aaatgagctg tttgaaaaag aaaatatcgt ttatcatcat
     7141 gatccgcaaa atcctggttt agcggtggct tataattttg cgttgtctca agcagatgaa
     7201 ggaacacgtt gtctcgtcac tctggatcag gatacgaggc tagtcgacga ttattttgaa
     7261 ctattgcgta aagtaacctt tacagatgaa tgtgttgctg cagttccgat gattttttct
     7321 gggagccgac aagtttctcc cctatattca gatcgttata tcaatcgtac agctcaagca
     7381 gtagaagttg aaggcccaac agctgaacga atcatggcaa tcaattcagg tgttgcctgg
     7441 tctgtcaaat ttctaaaaaa gattggcggg tttaatcctg ctttttcttt agattttttg
     7501 gatcattggc tgttttggaa agtggatcag ttggaaaaaa ctgtggagat ccttcctgta
     7561 cgaatggaac atgatctttc ggttttagac tatgaaaaag tttcaagtaa tcgataccgt
     7621 tctattttac aagcagaaaa tcgattttat catgaatatg atcaaaggca tttatccagt
     7681 caccgacgac aattattgtt aagaacagct aaacagttct tgaccgtcaa aaaccggcat
     7741 atctggcgga tgactttgcg ctcatatgtt aagaattgga aggtgtgaat tcgtgcgttc
     7801 ggtttgtata gctaccttta atggaggcag atatttaaga gagcagttag atagtatttt
     7861 atctcagctt tcagcagaag atgaagtagt catttctgat gatgggtcag tagatgatac
     7921 atgggatata ttaactgaat acgcagaaaa agataagcga atccagcttt tttcggggcc
     7981 tcgtcaagga ttgatcgcta attttggtta tgcgatccag cagacgaaag gagatctaat
     8041 ctttttggca gaccaagatg atgtttggtt ggagacaaaa gtgaaaaaga tcactgacta
     8101 ttttgagcag aatccggatc agctagtagt agtttctgat ttaatcattg tggacagcga
     8161 gctgcaagag atccatgatt cgtatttttc gtttcgaaat tccaaagaag gatggcttaa
     8221 taatgtgata agaagtcact atattggtgc tggtatggct ttccggtctt ctttaaaacc
     8281 cgatattttg ccgatcccgt cagaagtgcc gatgcatgat atgtggattg gtttgctggg
     8341 aggaaagaag actgggttcc taagagaacc gttaactctg taccgaagac atgatttaaa
     8401 cgcgagtgag attgaaacac attctaaatt acggcaaaaa atttcttggc gtgttcatct
     8461 gattacggcg ttgatcaaac gaaaatggtt taaacgttga taaaagatgt ttaaatcctt
     8521 aatatttcca tgacaaatat aacaattgat gagataataa caaagtgaaa tttgaaatca
     8581 aaaaggagag cagatatcca tgaagggaat cattcttgct gggggcagcg gaacacgtct
     8641 gtacccttta acaaaggcaa catcgaaaca attgatgccg atttatgaca aaccaatgat
     8701 ttattatcca atgtctactc tgatgttggc gggaatcaaa gaaattctga ttatttcaac
     8761 gccacaagat acaccacgtt tcaaggaatt atttggaaac ggaaatgatt taggactgca
     8821 gatcgaatat gcagtacaag aaagtccaga tggattggca caagcgttta tcattggaga
     8881 agaatttatt ggagatgata gtgtctgctt ggtattaggg gacaatattt actatggtgg
     8941 cggtctgtct aaaatgttgc aacgagcagc ttcaaaagat acaggcgcga cagtatttgg
     9001 ctatcatgtc aatgatccag aacgctttgg tgtagtagaa tttgatgaga atatgcaagc
     9061 tttgtcgatt gaagaaaagc ctgctcagcc aaaatcaaat tacgctgtaa ctggccttta
     9121 tttttatgac aatgaggtaa tttctatcgc taaaggtatc aaaccttcag aacgtggaga
     9181 actagagatt acggatgtca ataaagcata tttggataaa ggaaaactat ccgtagaact
     9241 tatgggacgc ggatttgctt ggcttgatac aggaacacat gaatcattgc ttgaagcatc
     9301 tacctttatc gaaacaattg aaaaacgtca gaacttaaaa gtggcttgtt tggaagaaat
     9361 tgcttaccgt atgggctaca tcgataagga acaattgctg gcccttgcac agccattgaa
     9421 aaaaaatcaa tacggtcaat atttattgaa tttggccgct gaataaggag tccttacaga
     9481 tgaaagtaac agatacaaaa ttaacagatg tcaaaatcgt agaaatggac gtctttggcg
     9541 atcatcgcgg tttcttcaca gaaagttatt caaaagcaaa atttgcagaa catggattga
     9601 aatatgactt tgttcaagac aaccattcac tatcagctga agctggtgtc cttcgggggc
     9661 ttcatttcca aaaaggagaa gctgcacaga caaaattgat tcgggtagtc actggagcag
     9721 ttttagatgt agtagtggat atccgcaaag gtagcccgac ttatggtcaa tgggacggat
     9781 ttatcttatc tgaacataat catcgacagt tgcttgtacc taaaggatat gcccatggat
     9841 tcgttacttt gacaccaaat gtgaacttcc tttataagtg tgataattac tataatgcag
     9901 aagcagatgg gggcatcgca tttgatgatc cagatttagc gattgattgg ccaattgatc
     9961 catcaagagc gattatgtcg gaaaaagatc accgtcatcc gacattaaaa gaatttgaag
    10021 cagaaaatcc atttgtttac ggagaaatct aagaattaag gagaaatttt cttatgaaaa
    10081 acatcattgt taccggaggt gccggattca tcggctctaa tttcgttcat tacgtggtaa
    10141 acaatcatcc agaagtgcat gtcactgttt tggataaact gacttatgca ggaaacaaag
    10201 agaacttggc cggacttcct agcgatcgcg tagaattggt cgtaggagat gttgcagatg
    10261 cagaattagt ggaccgtcta gtaaaagaag cggacgcagt tgttcattac gcggcagaat
    10321 cccataacga taattcatta aaagatcctt tcccatttgt acaaacgaat atcattggta
    10381 cgtatacact tttagaagct tgccgcaagt atgatgtacg ctatcatcac gtatctactg
    10441 acgaggttta cggcgatctt ccattacgtg aagacctgcc aggacatggg gaaggcgaag
    10501 gagaaaaatt cactgcggaa acaccatata atccttcaag tccatattct tctacaaaag
    10561 ctggttcaga tctgttagta aaagcatggg tgcgttcttt tggcttacgt gcaaccattt
    10621 caaactgttc aaacaactat ggtccttatc agcatattga aaaatttatt cctcgccaag
    10681 taacgagtat cttaagcggt attcgtccaa aattatatgg cgaaggaaag aatgttcgtg
    10741 actggatcca tacgaatgat cattcttcag cagtgtggct gattttgacg aaaggcagaa
    10801 ttggcgaaac ctatctgatc ggtgcagacg gtgaagaaga taataagacc gtgatggaat
    10861 tgatcttaga aatgatgggc caaccaaaag acgcatacga ccatgtaaac gaccgcgcag
    10921 ggcacgattt gcgttatgca atcgattcga caaaattgcg agaagagcta ggttggcagc
    10981 ctgagtttac gaacttccgt gatgggttgg ctgacacgat caaatggtac gaagaacatg
    11041 aagactggtg gaaaaaagaa aaagaggccg ttgaagcagc atatgccaaa aatggacaat
    11101 aatacagagg aaatttcact gtattaatgg aaaaacacat gtgaggcagg cgctcgcctg
    11161 tctctttttt aaaataaaaa tcaaggagcg gataaccata tgtttttatt aactggagga
    11221 aatggacaat taggtacaga attgcgccat ttattagatg aaaaaaatct gccatatgtt
    11281 tcaacagatg caaaagactt ggatatcaca gatgctgaga aaacaatggc ttatattaca
    11341 gaattaaaac ccgatgttat tttccattgc gcagcttaca cagcagtaga taaagcagaa
    11401 gaagaagcaa aagaattaga tgagaaaatc aatgtcgatg gcacaagaaa tgtagcactt
    11461 gcagcaaaag ctgtgaatgc acaattcgtc tatatcagta cagactatgt tttcgatgga
    11521 aaaaacaaaa atgtagaata cgaagtagac gaccaaacga atccactaaa tgaatatggt
    11581 cgtacgaaat tattaggcga aaaagcagta gaagagattt tagaagacta ttatatcata
    11641 cgaacttctt gggttttcgg gatctatgga cacaatttcg tcttcacgat gcaacgccta
    11701 gcggaaaccc gcgacaaact gactgtagta aatgaccagt ttgggcgacc aacttggaca
    11761 cgtactttgg cagaatttat ggtatatgtc atcgaagaaa aagcaccatt tggtatttat
    11821 catttgtcga acgaaaatag ctgttcttgg tatgagttcg caaaagaaat attgaaagat
    11881 acagatgtag aagttgcacc tgttacgtca gaagaatttc cgcaaaaagc aacacgtccg
    11941 cagtattcag tcatgagcct gaaaaagaca gaagcgttag gttttgtcat accaacttgg
    12001 caagaagcac ttgcgcaaat gctggaagct gccaaaaaaa tgaataagta atttcctttt
    12061 tatcaaatag aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga
    12121 aaagccaaaa gcgaatcagg cagattcgaa tttggtgctt gacctgtttc taaaactccg
    12181 ctatcttatt ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc
    12241 acttggtgtt tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat
    12301 ttttggacag aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct
    12361 ctcacaagct gaagaggact tttcgttggt gaacgaggat tattcattaa acggacaaaa
    12421 catgatcatt gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa
    12481 ctggggattc tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt
    12541 agtcgtcatg gtcttgcttg gattcgaagt actgatgata ttaacgaaga ggaaaaaagc
    12601 tctatcgcta atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat
    12661 gcaacatgtg ggcgatttga ttttctttac tttgggactg atgattgctt tttatcatta
    12721 tttctacaat catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt
    12781 cggtattggg tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct
    12841 gattttttat tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt
    12901 ggatgtagtg ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg
    12961 gctaacgtca aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt
    13021 aagtactgga ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat
    13081 tccttttgaa gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt
    13141 tccaactgta ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa
    13201 gttactggga cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg
    13261 gttgccaaaa tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat
    13321 ccttactttt agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag
    13381 agaagatgtt attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta
    13441 tttctatgcg attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc
    13501 agttgctgtg ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt
    13561 ttacctcgct tttgtcgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga
    13621 aggtacagga gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca
    13681 agatcctggt aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg
    13741 agtgaaatcc ttcaatacag tacgctttta tccagatata gctgcatgga agaaaatcga
    13801 tccgaaagaa gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc
    13861 taaggaagat acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt
    13921 tgaagacgca gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta
    13981 caaccaatta tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat
    14041 gatttttaaa gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa
    14101 tcagctagtc gtacagtaat aggctagctg agaataatcc tgaaatatta gtagaaagga
    14161 gagtgaagat aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct
    14221 tgcttttgtt gctgacgcta gtcttttttg tttggacagc aggggtaggt tttccagcaa
    14281 ctttgtttat atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca
    14341 ttttaaatgt ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc
    14401 ttttcatttt cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac
    14461 tgtttccttt gattatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg
    14521 atcgcggtat ttatctgatt gtctttacgg tcttcctcgg aatcacgtta tatcgtgtat
    14581 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    14641 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    14701 gtggatttgc cagttctttt gaacgatggt atgttcgaat catcggtggg atagcagtcg
    14761 ttgcagcctt tttaggtatt tgggtctgcc gatctagagg tgcattagtc gcgttgattg
    14821 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    14881 ttgcctacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    14941 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    15001 gagaaaaatc tgaacaagtt ttactaggaa tgaaaacatt tatcttccag cgaggaaacc
    15061 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    15121 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    15181 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    15241 tggaggatac attgacttca tacccatggg ttccgattat ttatatcctg ttggctatgg
    15301 ccgttcatcg atatgatgaa ccagaagtta cggaggaatt ctatgttatg gaagaggcat
    15361 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    15421 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    15481 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    15541 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    15601 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    15661 agatttgtat ttatttatga aggacattta tcagaacaga aaattactgc tacagttttc
    15721 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    15781 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    15841 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    15901 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    15961 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    16021 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    16081 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    16141 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    16201 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    16261 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    16321 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    16381 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    16441 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    16501 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    16561 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    16621 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    16681 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    16741 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    16801 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    16861 atcattgaat ttgctgacat cggcgaccat atttatcaac ctgtaaaaac ctattcaagt
    16921 gggatgttcg ttcgtctagc ttttgctgta gcgatcaatg tgaatcctga tattttgatt
    16981 gtggatgaag cattggcagt aggagatctt gaatttcaac taaaatgtat ggaaaaattt
    17041 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat tagttcgatt
    17101 cgccgttttt gtgatcgttc ctattggttg aaaaatggaa aagttgtcga atatggtgat
    17161 acgatggaag taacgacgaa ttacgaaaac tttttgaaga aaaaatcagt taaaacagta
    17221 gatcgaaata aaaaccaagt agaacgcttc gcagctccag atatcgtaga cgtgacgaaa
    17281 gccgaattgt tggataaaga taaaaatcca gtggatatgc tggaacaagg agaaaaacta
    17341 tacgttaaag taacctatga agtaaaagac gatacaatca aaaatccagt tttaggaatt
    17401 gctctgcgta cagtcgacaa tttttatgtg tgcggactca atactctact agatggtatc
    17461 aagatccctt ggaaaaaagg tgagaatatc ttttatctgg agtatgaaaa gttaggtttg
    17521 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    17581 gtatacaaga caaaatacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    17641 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    17701 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    17761 agttttagag tttggtcctg gaaacggccg attaacgaaa catttgatcg gagcaaaaca
    17821 atgtgaagtg agcattgtcg agctagataa ggaattgttc gattttgtca gcgagtttgc
    17881 ccaagatgga ttttatggag atatcgaaag ttttgaatgg gcaaactatt atgctggaca
    17941 aacatttgat tacgttttgt ttgccgatgt tttagaacat ttggtagatc ctggaaaaac
    18001 gttgaagaaa gttagagaat ttttgaatga agaaggagaa attctaatta cattccctaa
    18061 ccttgcgcat aattctgtca tgattgattt gttcaataat caattgccat gggcttctta
    18121 tggtttgcta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    18181 tgaaaaagca ggattataca tcaatattga agattatttg tacttagctg taggtgatac
    18241 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgttatgatt tcaaaatgcg
    18301 accatttggc gaagtctacc aatacttctt ctcattgatg aaacatccag tagcacaaag
    18361 ttccattgcg cagccgcaaa attctaatta tgtgaaagta ttagaagtaa tccagcaaac
    18421 gaaacaagat gaaataattc aacaaatacc attcaataat ttcacagggg aaaatgagac
    18481 actaagtttc catgtatcag aattagcaga acgaatggtg tttcgcttcg cgcagcaacc
    18541 tagctttatc gaattcagcg cagaagctgc tggagaaaaa ctcacattta tcgattcaaa
    18601 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat ggtaaagatt taccagagtt
    18661 ctttttaacg gatattgcag ggaaagaagt gactatccat tgtcactatc gttttatcgg
    18721 agaattgaca ccaacaatga aagaattgct ggaaacaatc cgtccaatgg ctgaagttac
    18781 aaagcagctt tctgagaaaa atgatgaatt agaaagagaa aatcattatc ttattgagaa
    18841 taatacaaga ttagatcaga cgttgaaaac aaccataaat cgctattgca cgttgttgga
    18901 aagtgatgaa tggacaatca aacatagatt agggcgacgt aagaaagaaa catcgaaaaa
    18961 aatccaagaa aaagagcttt ctctatgtat tgacgaaaaa atctgggatg cagaaacaaa
    19021 gatattgaaa atcatcgggt ggggaatcgc taattcggat cgccaaccat tgtcctataa
    19081 gttatcagcc gaccaatcac cattttttga agcaattcct gttttacgag aagaagtcaa
    19141 tcaagcaaaa cagctagctg aaggagcaaa agcaggattt gaactgcgga tcctttgcga
    19201 gaaggaacgt tcattcttag ttgaagcagt ggctcaaaat ggtcaatcat ggatcattga
    19261 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    19321 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    19381 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    19441 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    19501 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagcaatc
    19561 ttagacttcc aaacagcaag acatcaaaat ggcatagctg tgaaattaaa cggtaaatat
    19621 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    19681 ataaattatg caaggactca tggcgtaaaa aaagcaatcc gccgggtcaa actggaattg
    19741 aaaccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc tgatctgaaa
    19801 gagcaaagga aagaagtaca aggatttgcc taccgcccat tgatttccat acttatgcct
    19861 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact tgatcaaacg
    19921 tatgatcact gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    19981 tgtctagaat cctatcaagc aaaagatgat agaattaaag tcgttttccg acaagaaaat
    20041 ggacacatca gcctggcaac taattctgca ttggaaatgg cagaaggaga gtttatagcg
    20101 ctcttagata atgatgacga attacctcca tttgccttat atgaagtggc aaaagtgcta
    20161 aacgtacatc cagaactgga tttgatctac agtgacgaag ataaaatcga tgcagatggg
    20221 aatcgctttg acccgcactt caaagcagac tggtctcccg atacattgat gggaaataat
    20281 tatatctctc acttaggcgt atatcggaca agcattgtta aggaacttgg cggtttccgt
    20341 aaaggatatg aaggctctca agattatgat ttggttcttc gtgtgacaga acaaattcct
    20401 gcagaacaca tttaccacat tgaccgtgtc ttgtatcatt ggcggacgat cccgggatca
    20461 actgcaagca atggagaagc taaaagctac atctatgatt ctggagtgaa agcattgaca
    20521 gatgctctat ctagaagaaa tatcaaggga agcgtacatc ctggcagaat ttctggtttt
    20581 tatgaaatca cttatgatgt tttgcaagaa gacttggtca gtgtgatcat tccaactaag
    20641 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttatcca
    20701 aattatgaaa tcattattgc agataatgga agtacagatc ctaaaatgca agaattattt
    20761 gcagaataca agcatcagct gaaagaccga tttatcgtcg aattaattga cattccattc
    20821 aattattcac gtatcaacaa tctggcagca gaaaaagcaa acggcaaata ctttttattc
    20881 ttgaataacg atacagaagt gattgaacct gactggatga cagcgatggt ttcttatgca
    20941 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    21001 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    21061 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    21121 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    21181 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    21241 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    21301 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    21361 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    21421 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    21481 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    21541 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
    21601 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
    21661 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
    21721 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
    21781 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
    21841 cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa
    21901 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
    21961 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
    22021 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
    22081 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
    22141 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
    22201 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
    22261 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
    22321 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
    22381 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
    22441 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
    22501 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
    22561 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
    22621 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
    22681 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
    22741 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
    22801 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
    22861 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
    22921 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
    22981 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
    23041 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
    23101 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtgatt
    23161 aggagtgtat atatgaataa aaatggagaa tggagtgctg cacgacggat attcattata
    23221 ttaatggatg ttattgtata caatgcagca atatatagta gtttcttact gaagtttcgt
    23281 ggagagatcc cattacgaaa tttagaaact ttccaacact cagcaattat tatttcagtg
    23341 atttttattg cgttgaacat tttgttgggt gcatatgttt tttataatcg aatgatcagt
    23401 gacatcgtgt ttgtcacagt catcggtcag gtattgatga cactagggat catggtagtg
    23461 acttttgcgg gccgctggtt tgcttttcca cgtcttgtta tcttactttc atttgttctc
    23521 ggtacgcttt tcttaagtat ctatcgtatt ctcgtatata aactttattt acgcgtaagt
    23581 cgagataaaa aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc aatccaaaat
    23641 tttggttcaa agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat
    23701 gcaaacatca aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa
    23761 gaaactgaaa aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg
    23821 aatacaacat ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa
    23881 agtattattg aagcatctgg atttagaatc caacctgaat atgaactatt caaacgtttg
    23941 tttgatatca tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca
    24001 gcaattttag tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca
    24061 aaaaatcaaa aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca
    24121 aaatctggac cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt
    24181 attcgagctg tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg
    24241 tcgatcgtag gaccacgtcc tgaacgtcct ttcttcgtcg atcagttcaa tgcagaaaac
    24301 ccttattact acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat
    24361 ggaaaatatg tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa
    24421 tattcattga tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa
    24481 atgtcctctc aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt
    24541 ccgaatgaaa aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc
    24601 ataatcgtac cagtatacaa tgcagaagca actattggtc gaacgattga tattctaaaa
    24661 gaattggatt atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca
    24721 caggaaatca ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc
    24781 aatcaagggc ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc
    24841 ttattcttcg atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg
    24901 ctagaaaaga gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac
    24961 gagggaagaa tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac
    25021 gaagagttta taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg
    25081 aacaaatgct atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat
    25141 agttgtgagg atcgaatatt taacttacat tattatgaat actgtcatca agtgatcatg
    25201 aacccaaaag tagaatatat ttatgaattt gaaggtggga aggggatcac taatcaatac
    25261 cgacctaaca agttcgaaac atttaaagaa ttctattcat tagcaaatac tgtaacagac
    25321 gatattaata aagacggtat ggcggcgctt cttttaaaag ggacaacttc agttattttt
    25381 tctatttata gtacctctgc aagaacttca aaagagaaga aagacgaagc aaagcagata
    25441 ttgagtgatc cagtgatccg agaagccaag caaattgcta agacggattc ttcagcaaaa
    25501 aaaataacaa agttgcttta caatatgcca gctccactat ttttatctgc tgtaaaagtt
    25561 ggaagcttcg ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg agtatattag
    25621 ttaatggagg aaaatataat gaaaagaatt ttgcattttc agggacgaat gggtttaggt
    25681 ggagcagagt ctttcatgat gaatttatat cgtaaaattg atcgtacaaa ctatcaattt
    25741 gattttttga tttatgaaga ttatgcagat gttcaagact atcattcaga gattgaaaga
    25801 ttaggaggcc gtatttttgt tgtacctaat cctaagaaaa atatttttaa atatttaata
    25861 gaagtgaata agttactaaa aaaagaatct ttttctatag ttcacaatca ggtttatttt
    25921 ggcgggggaa ttaatttatg gttagccaaa aagaatggaa tcagacagag aattgctcat
    25981 agccatgcaa ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt
    26041 ttaaccaaat tgttgttaca aaatgcaaca gactaccttg ccgttagtca agaagctggt
    26101 gaaagtctgt ttcaaaacca tccctttgag atagttcata atggtattga tttagagctt
    26161 tatagtaaaa attcagaagc taaggttaat aagagaaaag aattggatat ctcaatgagt
    26221 acttttgttg tgggaaacat tggacgatta gagaaacaaa aaaatcaatc ttatttgtta
    26281 gagatttttt tagaaatact aaaaatgaat tctgattctt acttgatttt agtaggtgaa
    26341 gggtcactta gaaataaact gaaagccaaa gcagtagaat tggatataga gaaaagagta
    26401 aaatttttag gagaaagaaa cgatattccc gagttattat ctgtgatgga tgtttttgta
    26461 atgccttctc tttatgaagg tctgccaatg gttggattag aagcacaagc agcaaaatta
    26521 aaattagtct tagcagatac tatttccaaa gatacaaagt taacaaataa tgtggatttt
    26581 gtatcattag atgaaacacc acaagaatgg gcaaaaaaag taataaaaga gccgtatgct
    26641 aataaaataa cacaagaact aaagagttat gatgtttcgt acactactga acaaatggag
    26701 agtatatata gtagagatca ttaaaaatgt tcaagatatt ttaggagtga ttatttttga
    26761 aaatatgtta tttgatattg gcatacaatg atattggcaa cttaaagaga ttggtcaatc
    26821 gattgaacga aaatgcagtt tttattattc atatagataa aaaaactgat atagaaccat
    26881 ttattgaagc atttaaatca tatgataatg ttgaattttt atgtaataaa aaccgtgtta
    26941 aaatttattg gggtggtttt tccattgtac aagcagaaat caatttagta aagagagcat
    27001 tacaaaatga gaaatattta aaatatgtat tattatctgg tgctgattat ccaatcaaag
    27061 ataatgagta tatttataat tattttaaaa agaatagttc agtggagttt attcgtggaa
    27121 tagatctaga tcaaattaaa cataaagaat tttattataa acatatagat gtatatcaaa
    27181 aacatgatta tcctagaatt aataggaata acacaactgc atttaaaatt tttagagcta
    27241 taattaatcg ttgtctgagg atgattaaac ttcccccaaa aattagacac cataaatttg
    27301 atttgtatca tggttctcaa tggtgggctt tgtcgaaaga atgcctaaca gaattaatac
    27361 aaatgtatga acaaaatcaa caagactacc tgaattttaa aataggtatg tttgccccag
    27421 atgaaaaatt ttttcataca ctttttttta attcctcgtt caaaaataaa aatgttattg
    27481 gaggaccaga tttaccactt gaattgaaaa atattgaaga aactacttta caaacttcga
    27541 aattagcaaa tattcatatt attgatcctt ccatgaacaa atggtttgat gaaagggata
    27601 tagatcaaat aaaagaaagt gaaaaattat ttgtaagaaa agttaaatcg gattactctg
    27661 ataaactatt aaatagaatt gataaagaaa ttttgaaagt tggaaatata gatgaataga
    27721 atagtacctg ttttagagaa gagtttatta agtcgagatg cgtttcgagt aataaattta
    27781 atcatttgtc tgctttattt tatgcctagt acttttaatg taactaatat cccgatgaaa
    27841 atagcttttg cttggggtgg aattatagtt ttgtatgagg tttttataaa gaaaaatttt
    27901 tttaaaatgc gtttttcttg gctactgatt ggagcaatga ttagttttat cttaactata
    27961 atcattaaca gagaaaattt tttaattcca tctatttata attttggata tttgctgatt
    28021 actttgatag taatttttcc tgcagacttt tcacaaagtg aagaggaaaa aaagaaaaaa
    28081 atggtgtatt ttaataatgt tttcattatc ataatttttt tagctgcgct aatatctatc
    28141 tatcaattta tttttttgat ttcttatcat gtcccaacag gaacgccagg attacttgca
    28201 agacaaggtt ttattgaaca tcggttattt ggtgtctata caagtccaaa tgttggagct
    28261 atgtttggat atacttctat tattttaagt ttaattagtg tattaatttc aaaaataaca
    28321 aaaacgattc gtgtttttta tatgatgaat tttatcgtac aaatatttta tattacactt
    28381 tcttcgtcta gaggtgccca ggtagtaatt tgttcttttt tagtattatt tcttttgttg
    28441 tttgccaatt caaaatttag aaagagcatt agagaagtac tacctgtaaa aatcaaatgg
    28501 atggtaagca tattattagt tctattggta tatttttctt ttggtactaa ttatgctaag
    28561 gattttttag caacaattcc tgtatcgatt gatagaaaag tagaagttat agagtcaaat
    28621 gaatcagata aaactccact tccagaaaaa gaagtaatat tacagcattc tagtgataac
    28681 tccgaaattt cagctggtcg ttttacgatt tggaaagctg cgcttaaagt tataagacca
    28741 actttgttat ttggttatgg agagaccgat ttctatggga atagcgatgc aaaatttttg
    28801 aaaaaagtag atcttgatac tacagatgtg aatgaactaa aaagagcaca tggaaacatg
    28861 cataatggat ttcttcaagt acttgtatca agtggtataa ttgcttttat atgtatctat
    28921 gctttctatg tgttaaatat ctttagttta ctcaaaacat tttttagaag taaagtgaat
    28981 tatgcgtttc ttggtattgt tttaattttt atactatcta tttttataga tgaaatggtg
    29041 gaggctcatg tacttttcaa taagcgcgat gtcatttcaa ttgttttctg gtattatttg
    29101 ggaagcattt cttacttgta tttaaaaaag gagaattaat atgaagacag ctttcattgc
    29161 ttggactcct ttacatttga ttaatatatt aaatgtttat caaaattttt accgagaatc
    29221 aaaaggagat ttatttattt attctgaatt tgaaaacgct agaagatata gtgagcgtat
    29281 aaaagaatta gggctattta atgatgttgt ttttgtgaat caccggaaaa tcggttcttc
    29341 ctttaaacgt aaaattaact tgttaactaa ccataatcaa tttttctcgg atgatttatc
    29401 cgtttatgat gaaattttta tacaggggga aaattatttc acaaaaatac ttttctccaa
    29461 tgtttacaaa aaaaatccag atgtaaaact aaattatata gaagatggtt tgggtactta
    29521 tttgaagaag agtatatttt caacaagtaa aaacgttcaa agagttttta aaatattaaa
    29581 taaaaatagc atcttcaaaa aaaaaattga cgattatttt gtctattgtc cggaattgtt
    29641 actttatgat gtaaaaaata taaaaaaaat tcccaaaata gatgaaaaca gtaaattgat
    29701 gaaaaacata caatcaatct ttgggataga agaagttttt gtcggagata aatgtatctt
    29761 ttttgatcaa cctttattat ctgatggtta tggcatagat gaaacagata ttttcgctaa
    29821 agtaaatagt atattttctg gaaaaaaaga aactttaatt aagcttcatc ctagagcaag
    29881 gacaaatcgt tatggtgaaa aaaacatatt gaattccagt ttaccgtttg aagtcttgct
    29941 acttgaaaat gacttctctt caacactttt gattagtcct atatctacaa tttctttttc
    30001 accatctatg atgtttgaaa agacgatcaa ctctattatg ttggctaaat taattttaaa
    30061 tgaatataca gcattatctg aagataaaaa agaaacattg acaaatattg ttcttttctg
    30121 tgaacattat aaccgtctat cagaagaaaa aattttactc ccagaaaact gggcagaatt
    30181 aagaaagttg gtagagtatg ggattattta acacactttc caagtcaaaa ttagttaagt
    30241 ccagtttctg gtatactata gggaattttt ttattaaggg aatttcattc ataactattc
    30301 ctatttttgt aaatattact actcaagcag aatatggttt gattaataat ttcacttcat
    30361 ttgtatcaat tttttcgatt cttgttgggc taagcctaaa ttcggcaatt aataatgcaa
    30421 gttttgacta taaagatgac ataaatgaat ttatgtcatc ggtactttct ctagctacac
    30481 tttcgttaat tgtgttctta ttaataggga acatatattt tttgttttca aatgcttttt
    30541 ttgatatgaa ccaacttgta tttaatttga tgttattaca aagttttgct agttttgtaa
    30601 ttagttatat ccagtcttat tttacgatta actttaacca ttttaaatat ttgattttat
    30661 cttttatatc tacttttttg aatattgcaa tctctgtagc tttgatgcag acattatatt
    30721 ttgatgataa atatgttggc agagtagtcg gcggaacact gtcatttgtg ttgataggtt
    30781 tagtaattta tttaagaata atgataaagg gtgctcattt ttttgataaa aaacagtgga
    30841 gatacgcttt aaaaatctct ttgccgctaa tcccacattc cttagcaaat atagttttag
    30901 cacaattcga tagaattatg attaattctt atttaggtcc tgctgcgggg ggaatatatt
    30961 cctacattag taacataggt attattctaa gcgtaatatg gatgtctact aataacgcct
    31021 gggttccatg gttctatggt gagatgagta aaaataatga aaaaaaagtg aagaaagtta
    31081 gtaactatta tttagtagtc tttactttga ttacgatgat tgtaatggta gtttcgattg
    31141 atttagcaaa atttatggct ccgaaagact attattctgg gttatcttta gtggttccga
    31201 taaccttagg ctatttcttt cagtttttat atagtttacc agttaatgct gaattttatg
    31261 agaaaaaaac atcgtttatt gcgattggta cagtagcaag tgctatagtt aatatactat
    31321 taaacatgat tttcatacct aggttaggaa taattgctgc tggatacaca accgtattta
    31381 cgtatttttt actttttatt tttcattata atatcgctaa aaaaattacg aataaacaat
    31441 tatttgacac aaaaaaaata tgtatcataa taatttttgt atcaattttt tctttgatac
    31501 ttcatatttt aatcaatcaa atgttgattc gttacgtttt gacatcatta ttgataatta
    31561 gtttagttat atatttttta aagattaaga atcgaattat agatgactat aattcatagg
    31621 aggagatata agtgtttaaa ggaaaaacaa tattagtaac tggcggaact ggttccattg
    31681 gaagtgaaat tgttcgtcag cttttaaaat atgaaccaaa agctatcaga atttatagta
    31741 gaggtgaaga aaaacaattt tatatgcaac aagagctctc aaatcacact aatttgagat
    31801 ttttaattgg tgatatcaga gactctcaaa ggattgatta tgcatgtcgt gatgtggata
    31861 ttatttttca tgctgccgca atgaaacacg ttccagcaag tgaatataat cctatggaag
    31921 ctgtaaaaac aaatgtgatg ggaactcaaa atgtaataga tgcagcgatc aaaaataatg
    31981 taaaacattt tgtaggtatc agtacagata aagtagtgag tcctactaac accatgggtg
    32041 cgacaaagtt attgagtgaa aaattaataa tagctgccaa tcagtataaa ggaaaaacag
    32101 aaacaatatt ttcttgtgta aggtttggaa atgtcatggg gtcaagtgga tctgttattc
    32161 ctctttttag taaacaaata aaaaacaata aagatattac tttgacagat gttgatatga
    32221 cacgatttat gatgtcaata ccggatgctg caagattagt tctgtctgct gctatgaatt
    32281 caaaatttgg agaaacatat gtcttaaaaa tgccggcaat caatatttta actttagctc
    32341 aatcattaat aaaaatatat aatgatacac atgatgaaaa atatagaggt gaaataaaag
    32401 ttgtaggtat caggccagga gaaaaattat atgaagagct aatgactaca gacgaaattg
    32461 aacgctcata tgaaaatgat aaaatgtata tcattccacc acactatcat gctactgacg
    32521 aactgtacaa agattatgaa aaaattaaaa tcactgcata ctcttcagat gcaacagaat
    32581 taatgagtgg acaggaattt attagaatcg caaaagagaa taatgtcgtg acttggtaag
    32641 gagttttatg atgaaatcag aagtatatgt aattgcagaa attggtgtaa accataatgg
    32701 aagtctagat ttagctttag aaagtattga taaagctaaa gaatgtggag ctaacgcggt
    32761 taaatttcaa acatttaaaa cgaataagct agtttccaaa aaggctccaa tggcaaaata
    32821 tcagaaggat aatcttaaag aggacagttc tcaatttgaa atgctaaaaa aattagaatt
    32881 atctcaatct gattttatta gaataaaaga ttattgtata caaaataaaa ttgatttttt
    32941 gtctactcct tttgatgaag aaagtgcaga ctttctaaaa caaattggtg tacaaggatt
    33001 taaaattggc tcaggagatt taacaaattt accttttctt agaaaaatag atatgtatgg
    33061 gttgccagtt ttattatcta caggtatggc tgatattcat gaggtcagtg aggctgtaga
    33121 ttgtttcata gatagtccag ttactatttt acattgtact tcaaattatc cggctagttc
    33181 tgaagatatt aatttattgg cgattaacac attaaaagaa atgttcggga tacccgttgg
    33241 gtactccgat cattcattgg gatatgatgt tggtatttgt gctgttgcga tgggtgctaa
    33301 ggtaattgag aaacatttta ccttggataa ggaactacct ggtccagatc ataaagcttc
    33361 tttagatcct caggaatttg cagattttgt aaagcatatt agaaacactg aaatattttt
    33421 aggtgatggt gttaagcgag caatgccttc tgaacttagt acaaaagaag tatcaagaaa
    33481 aagcctagtg gtcactgaga atttaaatgt gggagatatt cttacagaga aatctcttac
    33541 aataaaacgt cctggaaatg gaatagctcc taagttttta tatcaatata ttggcaaaaa
    33601 agttaaaaga ccaataagtg cagatagtgt tctgcttgag gaagatgttg aatgaaaaaa
    33661 atacttatag taagtggaac acgagctgat tatggaatat ataaacctgt tgctgagaat
    33721 ttactacaag ataattggga tgtatcattt gttgtttctg gaatgcattt atcccataaa
    33781 tatggattta ctaaagaatt gatcttagct gacggattta gaattagtgg agaagttcaa
    33841 agtttgtttt tagagaacaa taagggaaac atggctcggt ctgtggcatt agagataaat
    33901 ggtttcacaa atatatttga acaaactagc cctgattttg tattattact aggggataga
    33961 ggagaaatgt tggcagctgc gatagcttgt ttgtatcttg gaatcaaaac agcacatatt
    34021 cacggaggcg aagtgtctgg tactgtagat gaatcaataa gacatgccat aacgaaaata
    34081 gcatctgttc attttgctgc tacggaaaaa agcaaagagc gtttaataaa aatgggggaa
    34141 gattcttgga gagtttttcc cgtcggagct cctagaatag atactatttt aaataataga
    34201 ttacctagtt ttagagatat aaaagaaaaa tatcatctaa aatttgaaaa gaaaaaatac
    34261 gttttgcaag tatttcatcc agttgtaacc gaactagaaa atatagaaaa acaaatttta
    34321 gctttgatca attcaatgga acaacaatct caaccaatta tttgcatttt acctaatagt
    34381 gatgctggta gcgaaattat cagaaaaatg taccagaaat atttaaaaac ggatattatt
    34441 tatattacga atttagagcc ggaggaatac ctgactatat taaaagagtg caaatttatg
    34501 atcggaaatt cttcttctgg gataattgaa gctgcatcat tcaaaatacc agtaataaat
    34561 ttaggaacca gacaaaatgg acgagaacaa tctaaaaata ttataaatgc aaatctaaat
    34621 gaaaatgaaa ttgaaactgc tttgaataaa atacaagatc ctcagtttat aaaaagcttg
    34681 aaaaatgtta agaacatcta cggtgacggc aacagttcta gaagaataac aaaaacattg
    34741 aatcaaatat tgaatgatgg ggaagatatt aagtggattc aaaaacaaat agcttattaa
    34801 aatatactag tgatccatac attattattg gggctggcgg acacgcaaaa gtcattattg
    34861 aaaccttaag attagaaaat aagttaatat atggattttt tgatgataag caaaataaaa
    34921 tgcaagatct gaaatatctt gggaaaataa acgaattgaa tagctgggcg gatagcaaaa
    34981 aaaaaatcta taaatttatt gtagctattg gtgactgtgt agtaagaaaa aagattataa
    35041 aaaaattgaa tatgccaagt gagcaatatg gaatagctgt tcatccaagt gctatagtgg
    35101 caaaagaaat ttcaattggt tatggaactg ttatttttgc gaatgctgta attaacccag
    35161 atgctgtaat tggagagcat gctataataa acacaggtag cattgttgag catgattgta
    35221 gaattaataa ctatgttcat ttatctccag gtgtatgctt agcgggtgga gtacatgtag
    35281 gtgtaggaac tcaaatagga attggttctc aatgtatcca gatgaagaaa attggatctt
    35341 ggtgtatgat aggcgcaggg agcactattg ttaaggatat tccctcacat tccttagctt
    35401 atggcaatcc agctaaaatt aagaaagaag gaattaattt tgaattctag aattttatta
    35461 tcaagtcctc atatgggagg aacagaagaa aaatatgttg ctaaggcttt taaagataat
    35521 tggatagcgc cattaggtga aaatgtggat aaatttgaag aaagtattac aaaatatact
    35581 ggttctaagg gcggagcttc tgcgttaagt tcaggtacag ctgccataca tcttgcctta
    35641 agactgctta atgttggaaa aaacgatatt gtctttgctc cttcatttac ttttatagct
    35701 acagtaaatc ctattttata tcaaggggct gtaccagtgt tcattgactc agaacctaat
    35761 acatggaata tgagccctgt tgcactagag agcgcattag aagaagctga aagaaacaac
    35821 caattaccta aggcaatcat cgtagtgaat ttatatggtc aaatggctga ttatgaacga
    35881 attttagcta ttgcaaacca atttaatgtt ccagtgatag aagatgcagc ggaatcgtta
    35941 ggttctactt ataagggaat ttcgtcaggc ttatttggga caattgggat ctattcattt
    36001 aacggtaata agatcatcac aacctcaggt ggaggagctc tagtgtctag tgataaaaaa
    36061 tatgttgagc attcaagatt tttagcaaca caagcaagag atcaagcacc gtattatcaa
    36121 catagcgaaa taggttataa ttaccgtcta agcaatattt ctgccggtat cggacgtgga
    36181 caaatggaag tattgaatga tagagtagct aaacgaagag aaattttcaa tcgatataag
    36241 gaagcttttt ctggaattga tagaattgaa atgatgccag aagtggatgg catggtgcca
    36301 aatcgttggt tgtctacttt aacgatcaat gattataatt ctattgatca agtagttaac
    36361 gtaataaata aactaaatga aaaaaacatt gaaacaagag ctttgtggaa accactacat
    36421 ttgcaaccag tatttcaagg ggtaaaaatg tattaccatg ataataatct tagtgtgtca
    36481 gaaaagttat ttaatagcgg cttatgctta ccttctggaa gtaatatgac agatagtgaa
    36541 caagaatttg ttattgatga gtttaaaaaa atattcaagt aggtgtgata ttttatgaac
    36601 ataggaatta ttggtttagg ttccatgggg aaaagaagac ttagactcat tcaagaacac
    36661 tttaatgata ttttactatg tggtgttgat actaatgagg cgagacgaaa ggaagttgaa
    36721 aaggaatttg gaataccaac ttataatagt ctaacagagg caaaaaattt gtttgaaata
    36781 aattcagttt ttgtttgtac atcccctata acccatgaat ttattataaa agaggcttta
    36841 aatttagatt gtaatatctt tacggaaatc aatttattaa atgaatatta tgatgaaata
    36901 atcgaagtag ctaaaaagaa agatagatta ctttatctat cttctacatt tttgaagaga
    36961 aaagaaattc aatatattaa gtctaaagtg acgcataata aaaaaatttc ttatcaatac
    37021 catgtaggac agtatttacc cgactggcat ccttgggaag attatcgcaa tttttttgtt
    37081 tcaaacaaaa aaactaatgg gtgtagagaa atattcgcta ttgaattacc ttggattata
    37141 gatacatttg ggaaagttaa atcgtttact gtaatgaccc agaaattatc caatcttgat
    37201 attgattatt cggacagttt cactcttatt attgagcatg aaactggtat agttggaact
    37261 ttaagtgtag atgttgtttc aagagtagct aaaagaaatt taactataat tggtgaaaag
    37321 attttaatag aatgggaagg taacccggat tctctatttt catggaatga agaaacttct
    37381 agtatgacag atattcaatt gtacgatgag gttatgcatg ataataatta ttcacatact
    37441 attattgaag atgcatattt agaggagatt aaagaatttt tcacgctttt ggaaaagaaa
    37501 atggaacccc aatatacttt tgaaaaagat aagtatatta ttaacttaat taacgacatt
    37561 gaggtaaatt aatgatgaaa gtaatttttt ttgggttagg gtcaattgga aagagacatc
    37621 taaaaaatct aataagctat ggaaaaaaga attctattaa ttttgatata acagcttata
    37681 gtagcagtgg aaaaaaagag tttgaagaaa taacgtatat tcaaaataga gacgatttgg
    37741 atacacatta tgatatagct tttattacaa atccgacttt taagcatctg gaaacactaa
    37801 atgtaattgt tgaaaaagtt gattacatat ttttagaaaa acctgctttt tctgagagta
    37861 ttgatcttac tcaatataat tatgattaca aaaatattta tgttgctgct ccactgaggt
    37921 ataaaaatat catggaagtt gttcgtaata gtattcaaag taaaaaagta tattctgtaa
    37981 gagctatttg ttctacttat cttcctgatt ggagaaaagg tactgattat agaaataatt
    38041 attctgcctt taaatctatg gggggaggag tagaattaga ctgtatacat gaattggatt
    38101 atattataaa cctatttggt ttccctaaat cagtaaaaaa acagtttggt aaatattcga
    38161 atcttgaaat taacagtaat gatttagcaa catatatatt gagatacgat gataaaatta
    38221 tagaaattca tttagattat tttggaagag ttcctacaag aaaatttcaa ttgtttactt
    38281 tagatgaatg tatcaatgtt gacttattaa ataataaaat aagttttgaa gaaaacattg
    38341 ttaaagaaat gcctgaatct gctaatgata tgtatgagaa ggagttgaaa tatttctttg
    38401 aaaacgtagt aacaggcaaa gaaaactgga atgatttata tcatgcaaac gaagtattga
    38461 aaatagctga ggagaaataa atggaaattt tatttactat ttgtggtaga gctgggtcta
    38521 aggggtttaa aaataaaaat ttgaaatatt tggcaggaaa gcccctagtc catttcgctt
    38581 tagcaatgat agatatctat caacaaaaac atcctgaaca taattgcacg acttgtttga
    38641 atacggacag taatgagtta gtagaattga cgactaagtt aaatccgtct gtgaaaattg
    38701 tagaacgtaa acctgaatta gctacagata ctgtagggaa aattgaagtg atacgtgatt
    38761 cattcttaca actagaaaga gaagataata tatttgattt aattatagat ttagatttga
    38821 cttcaccttt acgtgaagtt tctaatcttg aagaattaat agatgaacat ctaaaaaatt
    38881 tagataaaga cgtctacttt tcagttgttt ctgcaagacg taatccctat tttaatatgg
    38941 taaaaagaat tgaaaatgaa gttactatag ttaataaatc aagatacaca tctaggcaac
    39001 agacacctga agtttttgaa atgaatgcct caatgtattc ttttaaacct gaatttctaa
    39061 aaaaaagtaa ttatattttt gatgggcaat gtgggataat aaaaatgaga gattatctaa
    39121 ttttagatat tgatagtgaa gaagatttta aatggttaga atttttgttt cctaaatttt
    39181 tagaagattc ttcggagctt caagatatct ataattattt atatcggagg taaaaggatg
    39241 agctgttatc ttatcaccgg tggggctggc ttcattggtt ctaccttagc caatcattta
    39301 ggagaagaac atgtggtaat cgtcgtcgat gacttatcaa tgggaaaagt agaaaatctt
    39361 gatatgacga aaaatatcac ttttattgag ggagatgtcg ctgagaaatc cttgatggaa
    39421 gagatcatga aagctaataa atttgattat atctttcatt tagcagcagt tgctagtgtg
    39481 gcagattcag ttgcccgacc agtagaaaca catcgtgtga attttgaaag tgtattaatg
    39541 attttagaat tgatccgcaa gtatcaacct gatttaaaac ggatcgtgtt tagttcttca
    39601 gcggctgttt atggggatga accgacttta ccaaaaaaag aagagtccgt gattcgccca
    39661 ttgacgcctt atgcggttga taaattcgct gcagaacaat atgtgctaga ttattgtcat
    39721 ttatatgatg tacccggtag cgcagttcgt tttttcaatg tctatggtcc aaatcaaaat
    39781 ccgaattctc catacagtgg tgtgatttct attctcgtag atcgctataa gaaacaaatc
    39841 gtaggagaag caacttcatt tacattattt ggtgatggaa gccagtctag agactttgtt
    39901 tatatagatg atgtaatcca agcattactt ttagttgcga atgaggaagc tgcactgggg
    39961 aaacaattca atgttggaac gggtaaagca accacacttt tggaattgat tcatgcaatc
    40021 aatgaaattc tcggtgtaga attgacactt gaatacaaag aagagcgttc gggtgatatc
    40081 catgactctc tagcagatat tactaagatc cagtcaattg gctatcaacc gaaatatggt
    40141 attttaagtg gaatgactaa ttatttgaaa actgaaatca attaaaacat actaagataa
    40201 gattagaggg taaattcagg ctctttgtca ataaggactg atgagttttg taaaatcaaa
    40261 tctgggata
//
LOCUS       DACECR010000014        37938 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.014, whole genome shotgun sequence.
ACCESSION   DACECR010000014 DACECR010000000
VERSION     DACECR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.014"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(348..1292)
                     /locus_tag="IWO17_000697"
     CDS             complement(348..1292)
                     /locus_tag="IWO17_000697"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0289594.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1588..2532)
                     /locus_tag="IWO17_000698"
     CDS             complement(1588..2532)
                     /locus_tag="IWO17_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0289595.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2753..3967
                     /locus_tag="IWO17_000699"
     CDS             2753..3967
                     /locus_tag="IWO17_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0289596.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3989..6004
                     /locus_tag="IWO17_000700"
     CDS             3989..6004
                     /locus_tag="IWO17_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ0289597.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6087..7460)
                     /locus_tag="IWO17_000701"
     CDS             complement(6087..7460)
                     /locus_tag="IWO17_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0289598.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7486..7770)
                     /locus_tag="IWO17_000702"
     CDS             complement(7486..7770)
                     /locus_tag="IWO17_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0289599.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7772..9787)
                     /locus_tag="IWO17_000703"
     CDS             complement(7772..9787)
                     /locus_tag="IWO17_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0289600.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9966..10271)
                     /locus_tag="IWO17_000704"
     CDS             complement(9966..10271)
                     /locus_tag="IWO17_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ0289601.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10360..11391)
                     /locus_tag="IWO17_000705"
     CDS             complement(10360..11391)
                     /locus_tag="IWO17_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0289602.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11598..12473
                     /locus_tag="IWO17_000706"
     CDS             11598..12473
                     /locus_tag="IWO17_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0289603.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12625..13467)
                     /locus_tag="IWO17_000707"
     CDS             complement(12625..13467)
                     /locus_tag="IWO17_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0289604.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13559..15436)
                     /locus_tag="IWO17_000708"
     CDS             complement(13559..15436)
                     /locus_tag="IWO17_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0289605.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15433..16854)
                     /locus_tag="IWO17_000709"
     CDS             complement(15433..16854)
                     /locus_tag="IWO17_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0289606.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16857..20246)
                     /locus_tag="IWO17_000710"
     CDS             complement(16857..20246)
                     /locus_tag="IWO17_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0289607.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20503..21894
                     /locus_tag="IWO17_000711"
     CDS             20503..21894
                     /locus_tag="IWO17_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0289608.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21967..22614)
                     /locus_tag="IWO17_000712"
     CDS             complement(21967..22614)
                     /locus_tag="IWO17_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0289609.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22622..23308)
                     /locus_tag="IWO17_000713"
     CDS             complement(22622..23308)
                     /locus_tag="IWO17_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0289610.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23314..24387)
                     /locus_tag="IWO17_000714"
     CDS             complement(23314..24387)
                     /locus_tag="IWO17_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0289611.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24609..25283
                     /locus_tag="IWO17_000715"
     CDS             24609..25283
                     /locus_tag="IWO17_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0289612.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25288..26628
                     /locus_tag="IWO17_000716"
     CDS             25288..26628
                     /locus_tag="IWO17_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0289613.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26777..27763)
                     /locus_tag="IWO17_000717"
     CDS             complement(26777..27763)
                     /locus_tag="IWO17_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ0289614.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28487..29518
                     /locus_tag="IWO17_000718"
     CDS             28487..29518
                     /locus_tag="IWO17_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0289615.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29627..31159
                     /locus_tag="IWO17_000719"
     CDS             29627..31159
                     /locus_tag="IWO17_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0289616.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            31298..32110
                     /locus_tag="IWO17_000720"
     CDS             31298..32110
                     /locus_tag="IWO17_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ0289617.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            32100..32585
                     /locus_tag="IWO17_000721"
     CDS             32100..32585
                     /locus_tag="IWO17_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0289618.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            32608..33153
                     /locus_tag="IWO17_000722"
     CDS             32608..33153
                     /locus_tag="IWO17_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ0289619.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33226..34869)
                     /locus_tag="IWO17_000723"
     CDS             complement(33226..34869)
                     /locus_tag="IWO17_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0289620.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(34998..37226)
                     /locus_tag="IWO17_000724"
     CDS             complement(34998..37226)
                     /locus_tag="IWO17_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0289621.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(37259..37738)
                     /locus_tag="IWO17_000725"
     CDS             complement(37259..37738)
                     /locus_tag="IWO17_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289622.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        11061 a         7183 c         6905 g        12789 t
ORIGIN      
        1 tataaaaaaa ttttgcagtt tttcttactt tttttgtcgt ttaattgaat gataaaaacc
       61 agtttcttta ttcagttaat caaatatgaa cgaattagat atgaatcatt gtatttgccg
      121 ttgcggaagg attttttaat taaccacaga taaacattga taacgattcg cttattgtaa
      181 tcaacagatt aaatttcata tgttcatatg tacggataca ttttcggacc gattaatttt
      241 aaccattctg tttatttgct tacctattag atatcaataa aaaactgctt aacagctatt
      301 tcagctgtta agcagtttta ggatttttag ttcatattca ttgtttctta taatacagtg
      361 ttcaatactt ctcttaaagc acttgttgat ttgctgaaca ttgctttttc ttcttcgttg
      421 attgataatt caacaacttc acgaacacca ttttcgtcaa cgattgatgg cacacctgta
      481 aagatatctt tttcgtcgta ttcaccagtt aaataagcag atacaggcaa tacagcttgt
      541 tcgttattca agatagcttt tacgatacgt gcagtactca taccaatacc atagtaagtt
      601 gcttttttac gatcaatgat ctcataagca gcattacgga ctttatcagc aatcacatct
      661 aattcatctt gggcaatacg atggtctttt tctacaattt caaacactgg tttaccaccg
      721 acagttgtat gtgaccatgc agcaacttct gaatcaccat gttcacctaa gatatagccg
      781 tggacgctgc gtgggtcaac ttttaatttc aatgcgattt ctttacggaa acgagttgta
      841 tccaatgttg ttccagtacc gataacacgt gatgttggca gaccagattc gttccaagcg
      901 atatatgtca aaatatctac tgggtttgaa gcaacaacga taataccgtc gaatcctgat
      961 cccatgattt cttttacgat ttggcgcatg attgacgcat ttgttttaac taaatctaaa
     1021 cgagattgac caggtttttg gttgatacca gctgtgataa caacgatatc agcatcttta
     1081 cattcttcat agccacctga ccatacagcc acattttcat cgccccaagc cataccatct
     1141 aaaaggtcca atgcttcccc ttctgctttt tcttgattca catcgatcaa tactaattca
     1201 ttagagattc cttggttgat cattgcataa gcgatacttg ttccaacaaa acctgtaccg
     1261 acgattacta ctttacgact tgtttttttc atgaataaac agctcctttt cataactatt
     1321 tgttactttc atgaaaagta tagtcttgtt tattcatttt atcaacatgt taattataat
     1381 agttgttatt ataatttttg atggaaacga attcctgaat acgcataaca gataaatgac
     1441 aaatgtttac acctatatcc cctgataata caaccaaaag tttagaactg aaaataaatc
     1501 caaaaaaatt cgctgcaacg atataataag acagcaatcg cacctaaaac aattttcctt
     1561 catatgcttt agtgaaagat tttcttctta atgtttattt ctccattcat tgggagtaca
     1621 tgttgaatac ttcttgaata atttgaataa agaagtcgtt tgtttatatc cgatacgatt
     1681 agcgatttct tccaacgaaa aactcgtttc ttgaatcagt tccaacgcaa agtcgaattt
     1741 tttctttaga accagttctt gaaaggaagc atcaaaatac attttcaatt tattactaag
     1801 atagaccgga ttgtagccaa aatgagctgc tgtttctttt aacgtacaag aagtaaaatg
     1861 cttatcgata taatctaata ctctaagcat ctcattttct gcgcatccat ctgctgatga
     1921 aaagctttgt tgttctaata aatgagtcaa ttcgatcagt aatagtgatg tatagagatt
     1981 taacgaacgg atcttatgac tgtatgtatc aaaataaatc aacagcatat tttcaatcaa
     2041 atgcgtcaaa cgacttgttt ttccgccgcg aaaatgaatg aattgtgtat gttccccttt
     2101 ttcttttgat gcatttatca aaaaagaaga aagtagatta tttgtgcttg ccaaatgatt
     2161 caatatctct gtagataagg tttcttcttt taataagatg ttgattaaaa tatcctcttg
     2221 ccctgtgtat tcgattcttt gagtagcttc tttatcaatg atcaccacgt cactttcttc
     2281 catctctatc ctttctccat taataatttg taaacatgta ccgctataca tataattgat
     2341 ttcaacaaaa tcatgtgtat gctcgggaac ttccgcatat cgacggtgtt tactgatatg
     2401 aatatttccc tgttgaaata gatcccctcg gtggattttt ggcggtgtct gaagatggta
     2461 agctaagttt aaatcattga tattcattcc ttgttcacgc tgcagagttt cgattgattc
     2521 ttcgttacgc aaaaaaaatg tctaattcag ctagttccaa ttgactccct cctttttgta
     2581 agcggtttct ttcgcttatt agttcacgct gtactgctat tatatgcaaa gctatttttg
     2641 gtttcataaa gactttctat ggttgggaca tgaacttcct gtgattccgc tattgagaga
     2701 ataaccactt catactatat ttttgataag cgcttacaaa aatgaaagga acatgcttat
     2761 gaataaaaag atgatgaaat tttctctgct gtcagtctct cttattttga cttctgctgg
     2821 tgcagtcacg tctgcaattc ccagtatgtc agctacttat gccacgcgtt ctttatctgc
     2881 tgttgaatta ttaacgactg ctccctcttt tatggtcact atttttgttt tgattagtag
     2941 ttttattgct aggaaaatcg gacaaaaaca aactgtgatc atcggattag tgattgcatc
     3001 gatttgtggt atcctccccc tcttctcaca aagttacagt attgtcttag cttcaagagc
     3061 tggactaggt attggctttg gattgatcaa ttctttggca gtcagcatga ttgctgcctt
     3121 ttatcacgga gatgaacgag cctctatgat tggttttcaa agtgcatttc aaggatttgg
     3181 tgcagcttta atgacatttg ttgcaggaca gctagtacag ttcggttggc aatatacctt
     3241 tttagtctac gccatcagct tacctattct tgtcttgttt gttttgtttg ttcctacacc
     3301 tgaaacaact gaacttcaat cgcatggcac agccccaaca agacaagtaa tcaaaacgaa
     3361 tcctaaaatg attagttatg gtatttttct cttgctggtc atcgtggtat ataatgctgt
     3421 atcgattaaa ttagccacag tcctgacgac ttattctcta ggaaacgaaa ctaatgcagc
     3481 gaatatctta acaatcatgc aattggcaag tatggctgcc ggagtttgtt ttggctggct
     3541 tcagacaaaa gcacatcgct atatgcttac actttcttta ctactgatgg gaatcggctt
     3601 tgtattgatt gccactgtga taaacctgta tgtgattggg tttggtgcga tcctaacagg
     3661 aatttctttt tcgttattca ttccttactt attcaaccaa gtaagtatcc aagcgcccaa
     3721 gtacgcggag agtttcaata cttcaatctt attggttggc gctaatttag gtactttcat
     3781 tgcaccatat ggtttgctct ttctctcttt tgcttctctt gggagtaaaa cacttgctgt
     3841 tttcgtcaat ggcagtatct tgctgttgat tagcgcgctt attagccttc tagtagtaca
     3901 caggacgaat agaaagacag tgataagagt agatgaagaa ataaaagaac aattgacaac
     3961 aaaataaatt tgatggaggt cgtgtaaaat gacaaatgaa aaacataaag atagatggct
     4021 ttggtatcct ggagactttg agatccgcca tggactgcta caaaatttcc aacgggaaga
     4081 acgaggtttt gactggccag cgtactggta catggatgat tgccatcgga acgtcaagtt
     4141 taagagatat tactttcttg atcaaccttc catgtttaaa gtgacgatcc aaggagttgg
     4201 gtatgtagaa attaatggac aaaaacatcc atgtggaaaa tggctgactt gtccagctgg
     4261 taaagcaaaa attcgtatct ttgtaggaca tacatcaggg ctaccagcga tgtttatcga
     4321 gggagacgaa gtaaaatcag atattggctg gacagcaagt aattttattg aagaatatcc
     4381 agctggctgg agtccgcttt atgtcgatat cgcaaaagat ccaaatcaaa tctattatca
     4441 aaaagaatgc gtccatccag tcactgaaac ggagattggc ggaggaatct tattcgattt
     4501 tgggcgagca gtgaatggct gtgtagaact aatatctctg gatgaccata tcgaatctat
     4561 cactctatgt tatggagagt ccgatgtaga agctatggat atcgattatt gctactataa
     4621 gcaaatagga atgacaagaa aaatgaaagc tcgtaaacgc gcttttcgct atttgtttat
     4681 accagaggtt tctactggaa tggttcaact tcaagttttt catgaatttt tatctctttc
     4741 gaaacaagct gctttttcaa gcgattcttc tttactgaat cagatctggt cagtatcaga
     4801 agaagccttc aagttatgta gtgatttgtt ttttatcgac ggtatcaaac gagatcgctg
     4861 gatttggtct ggagatgcgt atcaatcatt catgatcaat caatatcttt tttttgacga
     4921 ggaaatcaat aaacgaacga tattagcatt aagaggacaa aatgaaatca agcagcatat
     4981 gaatactatc gttgactatt cgatcttgtg gttgattggt atcgaaaacc actatatgat
     5041 gacgaaagac tatgaatttt tacgcatcgt ctaccctaag atgcaatcat taatgagata
     5101 cttgatggag caaacgaatg aattagggtt tatttatggg agagcaaaag attggatatt
     5161 tattgattgg tctgaaatgg ataaagaagg aacgcttgct gcagaacaag tcttattact
     5221 gaaagcctat cattccatga tcacttgcgg agatatctta gctgaaacat atggagagaa
     5281 cgaaaattat catactcatc tctatcaaga aaaatatcaa ctattgaaac aaaacctatt
     5341 tgactacttt tgggatgaag aacttggcgc ttttatcgat tgctatgaat ctgggagaag
     5401 aaatgtgacg cgtcatgcga atattttcgc tgttttattc gattttgcta acaaagacca
     5461 acagcaatcg atcttaaaga atgtcttatt gaatgacact atcacacaga ttactactcc
     5521 atatttcaaa ttctatgaac aagatgcttt atgcaaactc ggacagacga atatcgtcta
     5581 ccaaacgatt cttgattatt ggggagggat gttatctcat gatgcggtaa ctttctggga
     5641 agaatacgac cctactcaaa caggaaatga aaagtacagt atgtatggag atccttatgg
     5701 aaaaagctta tgccatgcat ggggagcaag cccaatctat cttttaggac gctattttat
     5761 cggtttgtat ccaacaaagc caggttatga agaatttatg atacaaccta atctagattc
     5821 atttgaaaca ctgaattgta cgctacctat caaagatgga aagatttcgc ttgaaaaaac
     5881 aaaagataag atcgtgatta cttctgatag aacaggcgga acagtgaaaa tgaatggtaa
     5941 aagttatcct cttctagcaa aacagccact tgtcttaaca agctattcaa aaataaatgc
     6001 ataaaacaaa aaactgcttg cgacatatca gaagacttta aatcttcttc tgatattgtc
     6061 caagcaattt ttattttgaa gtttgcttaa ttctgttctt tagaaacaat gtttgttctt
     6121 tttccggcaa aaagaaagct agataatacg acaagtaaaa agaaagcgat gattccaact
     6181 gtcaaggcta tttgaccgta ttctcctagt ttcccaagta caagtcctgg tactagataa
     6241 tcagtatcgg caaatgtaga agaagcgatt ttaagatctc ctaatactgg aaggataaat
     6301 aatggcagcc aactaatcaa taagctattg aaagcagaac ctaataccgc ccctcttcta
     6361 ccgccagtcg agttgccgaa gacaccagaa gcagctccgc agaagaaatg tcctactacc
     6421 ccaggaatga taactgtagt ttgcaagcct atcatagcaa acatggatag tgttcctaca
     6481 aagaagctaa caaaaaagcc aattaataca gcattaggtg catatggaaa aacgatagga
     6541 acatctaatg ctggtttaga atcgggtacg agttttttag caatcccttg gaatgctgga
     6601 acgatttctg ccaacaccat tcgaacccct tgaaggacga taacaaaccc agctgtgaat
     6661 gttccagctt ggatcagtgc aaagattaca gcgttgtctc cattgcttag ttctctttca
     6721 acaaatccag gaccagctaa caaagcaagt gcgacatata cgatcgacat caagagcatg
     6781 atcgatacag tagaatctcg caaaaagctg aaacttttag gaatctttat gtcctctgta
     6841 gaacggtctt tgtttccaaa aaactttccg atccatccac tagaagcata accgatattt
     6901 ccggtatgcc ctaacgcaac ttgattgttt ccagtgatgt tcttcataaa cggttgacaa
     6961 agagcaggcg taatagttaa tagtgttcct tcgaagaccc cgcctaataa gatcatccac
     7021 aaatttgcag taaaccccgc gcttactaaa atgactgctg ttagacaaga aacataaagc
     7081 attgcttgac ctgtaagaaa aatgaattta tatcttgtga atctagcaag taaaatattc
     7141 acgatcatcc ctactagcat gatcaaagca gtaggtgtac cgtattgttg caaagcaatc
     7201 gccactactg cttcattgct aggaactact ccttgagtat gcagagctgt ttggaacatc
     7261 gaagcaaaag gggttagtga attactgatt aaaccagcac cgccgatcag tactaggaat
     7321 ccgacaaatg ttttgatagt tccttggatc acttctgaaa atttcttctt ctgaagaacg
     7381 agaccaagca gtgcaatcaa agaaacgagg atggagggct gactgacgat tgacacaaac
     7441 aaattcaaca atttatccat tgatagttcc tcccttgaat catttttatt cttttgacaa
     7501 gatcttcgtt actttttctt ttacttcttc gctgtcaatg atcgattgca aaggtacgag
     7561 cttttcatca ggtatagaag ttagcgtatc tttcaaagaa tgctcaatga agaaataatc
     7621 agccatatcg ctagatacac ttccaacatc cgtatgactt acttcaaact cgtccacatc
     7681 tgctccaaga tctgcaagtg cttgtttgat gttcatctct accataaaac ttgttcctaa
     7741 tccactttga catactgcaa aaattttcat atcattcgtc tccttttctt atgatcgcta
     7801 atatttcttc ttttgttgat gcgtgtaata aatcttgaag attttgttta ttagaaagta
     7861 gctttgttaa atttgccaaa gcccgaagat gtgcctcatt atcgatagca gctaaacaga
     7921 tgaacaaact aatgggatgg gctggattat caagcagatc cacaggttgt tgaagtttca
     7981 gcaaagacat ccctacttcg tttacgccat cttctggacg agcatgaggt aatgcgatgt
     8041 gatcgccaat atggataaac gcaccatact gctccacgcg ctcgatcata gctttgatat
     8101 aactttcttc tattttattt tctgataata gcggctgtgc ggcaaatgtg attgcttctt
     8161 gccagttttt tggttgatcc gataattgga tcatttcttc tgttaataat tcagatagca
     8221 taggacgttg atcctccttc ttttctaaaa gtttgttcat tttacgattt aatacttgat
     8281 agagcttttc ttcgtctacg ccttctttta attcgatata aggcagtagc gccgacaaca
     8341 attcttgggc agaaggcatt gaaaatcctg gtatcagcca atctctttgt acttgattga
     8401 tcagctgatt tttttccaag tcagtcatga ttggatgcag aacatagaga gactttttct
     8461 tatcagtaag tgtgagaggt acagtagaaa aaataatatc gtaagaagtt tcgtcaacct
     8521 cgtgtaagcg gtcagctgaa ttagtctctg taaaaaagat catcgggaat aattgctgaa
     8581 gttcactttt taagatcaat gaagagctaa tgccacttgg acagacgatc acggctctta
     8641 tttttctatt tctttcttct gcgtccgctt ttctgatctc tccgccaaat agtatagtga
     8701 aaaaaccaat ctcttcttca gggatgctct tccctattcg tctttctaac ggaagtaatg
     8761 cttttcttgt catttcgtag atggaagcat attgatgctt aatttgatcg atcatcacat
     8821 taggtaaata aaaagaatag gaaatacgaa aataggcagg gaccaagtga taaaagacat
     8881 tcatcaataa ttcacgaaac gaagcaaatt ctacggccat caggaattcc atgcgatgga
     8941 tgacttctga agaacattcc agcaaaaaat caagtgcagg ctcctggaca tctccttcaa
     9001 cgatcgatgc taagcaaaga gagaaaaata taatctccga attttgatgt tcggaatagc
     9061 caaggccagt caatacttgt cgactaatgt cagtgactgc aagagaagcc aacagttcag
     9121 catcttttac cagttccatg gatgtttttc caattcgaat ggttgtcaaa ctcaaataat
     9181 aagctaattc atccattcga tttggtgcga atcgaagatc tgatagtgaa atcgttttat
     9241 ctagcaaatc tctaactttg gcgtaataat caatggaaag ggttgcggtt aaatacgtga
     9301 gcccaaatct tccagcaagt gactgcatca aacgagcagt gcgatttttt gcttctcttc
     9361 ttaatattgc ttctgaccct agcagcctaa aaccagtttt cctagtatac gacagctgac
     9421 aatcttccac ttctaattca tttcttatct ttttgatatc tgacaggact gtccctttag
     9481 aaacctgaag aaaatcttga aaatgaaaaa cagaaacaga cgttgcagtc gaataaacca
     9541 tcaaataaag aagatctctt cgctcttgtt cggtatacac agcttcaaat cgagtaatat
     9601 ccaaaaattt ttctgcccag tcgattgctc ggaatttctt tatacggata cgggtatcat
     9661 ccacatattc ggcaaaagga gttaattgtt tgatcgcctc gcttgtattt acttcgatga
     9721 tctctgctgc tttttctaga ctgatttctc ttggtgtttt caggagataa ctaattttgt
     9781 agttcacagc gctgttcacc tcctacttat attctagtat ggtagcgctt ttaaatcgag
     9841 gggtgtttgg tacaacttac agttggattt tttttacgac tgtaccgaac gatttctatc
     9901 gaatggatac agaaaaaaag caggtgacca aatgtctcct gcttttatga attgatttcg
     9961 tccgatcaat gatgatcatg tttatttttt gaggtcagtt cttcaaattt ttctttgaca
    10021 tcttcaaccg ctttttcgat atctttttca aagtgattgg gctcattagg ctttgtttcc
    10081 tcttctacag aatttgaacc agataaagct gtttctttat tttctgtttc accagagttt
    10141 gtgccagcta atgattcttc tttgtcttct gtttcaccag aattagttcc tgctaaagcc
    10201 acttcttctt ctgttttagg ggccattgaa cctcttaatc gtttgtgttt ttcaccgtca
    10261 taatttgtca tgttggttcc tcctagtagt tctatacttt ttcatcgtac aataagacag
    10321 tttatgcgtc aaaaaacaaa cccgattctt acaaatatgt catatttttc cttctgattc
    10381 ttgaatgctt ttgattcctt cttccaaaat tttcatttgt aagattgttt cttcatcttt
    10441 gatgactttt tctgcgccat taactaatgt ctcgtaaatc ccttcgtaga cacggctgta
    10501 atcaccaacc tcggaaatta ctttttcttc atgataactc ccttgttcat ctatataggt
    10561 caatacacca taatgttcgg gcaaatcgat accaaagtct tcatgatcag gcatgtaaaa
    10621 cattttcaga tgttcttctt gtcgatcttt tgtttgtttc acgaacatcc ctttttttcc
    10681 gtaaacgacg aaacttggac gttctttgat gcggaaataa ctggatttta tcgaaatttt
    10741 catatttcca tagaaaaaat ccagatcgaa gtaatcattc attcgtcctt tccctaaaag
    10801 ttgacgtaca tcatagctga tatcatctgg ttcgccgaaa taagaaagaa cttgatctac
    10861 tgtatgacaa gcatgtccat ataaatagct gttcgcagta gaaaattcat gcgtattttc
    10921 tggtacttcc ggtctgaaat aatcataatg catttctact tctaaaatat cccctaattt
    10981 tccgctttcg attacttttt ggactgtcaa gaagtcagaa tcgaaccgtc tgttttggta
    11041 acattggata aatagacctt tttcttttgc taatgcaaat aactcttctg cttcctttga
    11101 cgtttctgca aaaggtttct ctactagtac attcttacca tgttcgagtg ccatttttcc
    11161 atattcataa tgaaattgac taggtgtagt gatgacaact aattggatct caggatcatc
    11221 ccaaagatca gacagctgat cagtatattc gatgtcttcg aatgttttcc acactggttc
    11281 agatggggaa aaaatttttt tgatattgat tttgttcgta agttttcttg aaaaaggcaa
    11341 atgatagcga tttgtactct ttccattgcc aagataagcg attgtaagca tgagtggtgc
    11401 ctccttttat tgatgttttc agtatatcct agaaaagcgc agaaagtatt tataatagct
    11461 attctggtaa aaaagttcag aaaaacactt gttatcattc ggtttggtga aattaatgtt
    11521 caaagtaagg ataaaaatca aaaagatagt agcggaacca agtgtttatc agtaagatag
    11581 aatttggagg tcagatcatg cttattgaag aaaaactaac aaaacaagag ttgtttacca
    11641 cgacagagaa acggattgtc gattacatcc gtaggaatat cgaagctgca gtctacatga
    11701 cgattgaaga attagcaaaa gctacgtata cttcccattc agctatcatt cgtttatgca
    11761 aaaaaatggg attcagcgga tttaaagaat tccgtttcga gctggggcgg gaagttcatc
    11821 aattgattgc tcagttcaat caaacgattg atcctaattt tcctttttca agtgaagata
    11881 gcactcagca aattgcaaag aaaatggcgg atttatcgat ccagtcaata aaaaaagcac
    11941 aattgcagat tgaacatgaa gatttagatc aaatcggtaa agtattgaac aaggcacagc
    12001 gaatctttct ttttgcaaaa ggtgattctc aaatcacggc aaggaaattc cagaataaaa
    12061 tggtcaagtt gaataagttt ttgatcatgg ctgaagaata cagtgattct tcttggaatg
    12121 cagccaattt gactagcgag gattgcgcca tttttattag ctacagcggt cgcatccatc
    12181 attatgaacg aataatgacc tatttgaagc atgtagaggc ccctaccttg ctgattaccg
    12241 gaaatcagca ttcagaaatg gccaaacagg ctgatatgtg tcttgttata tcgcaggagg
    12301 aatatgattt tgctaaagta gcgactttct cctctcaaat tgccttcgat tacgtattaa
    12361 atacgttatt ctcagtgata tattctcaaa attttgaaga gaatatccgc aacttgaaaa
    12421 acaaacaaga attgatgcaa aaaggccttc ttaaagaata tcggaaaact taagaaaaga
    12481 tcctcattta gtaataagaa agatagtgtt tgataggctc tctttgatat catcataaaa
    12541 acaggctgaa gttttctttt tcaaacctca gcctgtttgt ttacggaacg attcgaatag
    12601 atatattata gaaatgagga atagctaact tctgtttctt attttgaacg attgacgaaa
    12661 aagatatata agaaggagga aaatcagtat acatgcagct aacaatccat acaggaaaaa
    12721 tcgtttgttc tcggatactt tctgtacttc tttttgaact tcttttgcat gataaggaac
    12781 ccggtgccct ctgacaagta agcgatgact gttgaccata taaggtgtac aagttaggag
    12841 tgtcacgaaa tcctgccctt tttcgattct cagatctttt gtatcagtcg gttctaccgt
    12901 cttgatctga tcgacttgat aagcgagtgt ccgtccattg atttcgatat agaaagaatc
    12961 acctaaagtc agtttcggta agtctgtgaa caactttgct tgaggcagtc ctcgatggct
    13021 ggaaataacg gcatgtgtat tttctccccc cgtaggataa gacgttcctt ccaataatga
    13081 ggcacctttt tctagcagca cagtattcgt ttcatcaaaa attggcaaat ggacattgat
    13141 ctcagggatc gtcaaaatac cgatcgtatg tttctcaaaa taggttctat cctttttcgc
    13201 aggttctttt ttcttagtaa agggatctgc acctggattt ccaccttctt ttgctaattg
    13261 ctggtttttc tttgtcatgt tttcttggat tttagccatg acttcttcat tttctttgac
    13321 cgcttgttgc tgataatgag agatgatctg ctgatccaga taattattca gcgcgtcgct
    13381 aacaaatgga taagctagta tccctattcc tgagagaagc aagaaaagca tcagcagatc
    13441 tagaagtttc cttttctttg gtttcatttg tgttctcctt gtgatgactg tttgataata
    13501 aaagaaaaag gagcatttgc gtaatccgct gctccttttt ccaattcttg caaaaagact
    13561 aaatctgact gtgcttgcgt ctagcaaagt acagtccagc aatcagtaga aggactactc
    13621 ctgcaccgat atacacatag attcccttac cgcctgttga aggaagtgta cctttgtgtt
    13681 tatttggtat tttttcagga gaaatcaact gtcctgctgt tacataagat tgttcatcga
    13741 tagtaaatgc tacacggttt gttaatggaa catatttttc tggcgctttc gtttcttcca
    13801 gatagtacgt gccatatttt agacctgtca catcgattaa accgttactt gtggttgtaa
    13861 aaaccgttgc tgattctttc gccgataccc agctgacggc ttttgtggat gggtcgatcg
    13921 ataaatattt cgctgtgtca ctatcttgat cacgaacgac gaattctgct ccagcaagtg
    13981 tttggtctga tgtaacgtca ccatctactt taacaaatcg tttgccccca gtaacgacat
    14041 cgactgacgg tggtgtttga tcatttgtat gcccgttatc gacatttgct tggttagaaa
    14101 atcctttggt tggatctgct ttttcgttca aatgcatata gtaaacgaat ttcaatgtac
    14161 cgccaggagt taatgaagga atataattcg gatcaactga aaccgtgaat ccgtctgttt
    14221 gctcagtgac agaataattt actgggtcga tttctttatt tccatcatat aaggcataag
    14281 catacgttcc tgaagaatca ttatcaaatg ttaaagcagc gtcatgagta tcgataagtt
    14341 tgaatgttgt gtacttgttt tgcgttcctt ctttatcagc gatacctaat gggatattga
    14401 cagaaatctc atattgaatt ttttcaccga ttgcgacatc ttttccattc aattgaggtg
    14461 ttgttttatc aactttagac gtatcatttt tgatggtctt ttctactggt gtttggctat
    14521 ttgccaggat ctcaaaaggc gtgattgttt gcttttcaat catttccgca ttatcaggag
    14581 cttttacttc ttctaaatta tattttccaa cttctaaatg attaacgatc aactggcctt
    14641 tttcaataga tgcctcggca aagtcattgt tgccgatgtc ataagaatga ccagtaatga
    14701 aatgtttggc tttggtttga tcagttgtcc aagtgtacaa tccatctgtg acactttgga
    14761 tgtatttttt gacgcttggt gttccttctt ctttagaaat aataaattct gctccattta
    14821 gtgcttcgtt ttcggcagta ccgatttttg taactttcaa cgttccatca ttaccgactg
    14881 tatttttagg gtagagatgg atagtatcta gttcttctgt cccgtattta taagagccat
    14941 ctgcttgttt gatcatctca tatacaggga aagctaaaac catgtttgcg gcagctgaca
    15001 ctccgtcttt tggttcttct ttgatcgtgt agactgcatc tttcccattt tgttttttag
    15061 gtaaagataa agtgacattt ccatcagcat ctgtcgttcc tgaagcaact ggtgtaccag
    15121 gagtcaaaga ctggactgct tgttttgctg catccacgga cgctccttta tctcgttgcg
    15181 cataaaattc ttgagtgacg ttataaactg aaaatgaaat atcggctaat ccttggtatt
    15241 gatcgaattc gctcatttct ttcccgctgt tttggattaa aggatcgggt aaatcagtca
    15301 ttttcttttt atgaaggatg acttgagctg ctgtctcctc tgcaaacact ttttttgcgc
    15361 cgaagctgtt tgtgagtaat ggtaaaataa ggaaaaggac tcctaacata acgtttattt
    15421 ttttatgatt tttcattttt tcactcctgt tctttttttg ataaacaacc agattgcaaa
    15481 gcttgcgaat accataccaa caacgcctaa aagtgtattt ttcgtttcgt ttgtttttgg
    15541 aagaaatgga cggctatttt cttttttatt gactactttc aatatactgt ccgcctcttt
    15601 atcctcagaa agaaaagttg aaatggtaaa cggaatttct gtttcactta aaatataacc
    15661 agaaggtgct tcgatctctt ttaatcgata acttcctgtt ttcaacccgg aaattgaaaa
    15721 agcgccattt ttatcggaaa tcaggtgaag cgcatctgat tcgttctttg tccacttgta
    15781 tccgttggct gtttgctgga ggtaatttcc tgctttgttt ttaataagga atactgcgcc
    15841 agctaatcgt ttatctggtt tatttgtctc gattttgaca aattgcttac tccctgtata
    15901 tacagagact ttctttgtac tgacaccaaa accaaaatcc agtgttccgt tgttgttgat
    15961 ttccttgttc gcctgtgctg tactgcttag ctgcatctga taagaaatgg tcagtttttt
    16021 tccagcatat ttttccaacg aagggatcga aaataaaaca tcaaatccgt gaggtttctt
    16081 gtgtatgaca aagccttctg tcagcttttc tccattcgat gaaatcgtta aactattagg
    16141 taaaaacgtt aatgcttcat ccgcactatc tgacaattcg aacttttgat agtcaaggat
    16201 atttacagga attgtcgtat gcaaggaata agtgattttt tcaccaatcg taaaatcatt
    16261 tctaggctct tctaatacct tctcaaaagg tggatcaggg tagtcgtttt cttcattttt
    16321 aggatacaaa tggatagttg aaagtttttg attgttttgt ccatgtacag gaagaacaac
    16381 taccatattc tctgcctttt ctttgacgac ttctggtact ttggattcaa tgaaaagata
    16441 cgctttatct cttttttctt gatctttagc ggccagagaa aaagaagcga taccatcttc
    16501 gttgttaaga gttgtagttg ttgcttctgc ggtaaacata ccggaagacg caccgttttt
    16561 tgcgatctct gcttgtgctt cttctaccgt tttgcccttt tcccgtagat ggtaaaaaga
    16621 atctgtgaca tcataaactt ggaatgtgac accatttaat cctcgatacg tttgtaataa
    16681 agctttttct tgtccgtcat ttggatgatt tttcggcagt tgcccgtttg ggaaaagcaa
    16741 tttgtgtaaa gtaatctgaa ccatttctgt ttctgtttct gttgctacct gagtaaaagc
    16801 tggtttaaat agtaacaaga agagacacat actaagccaa ccaagttttt tcatatttag
    16861 ctgccccctc tttctttttt caaaaaaaga taccaaatag aaaacgcaca accaatcatc
    16921 cctactagat agattcctaa acggccaatt cctcccgttt caggtaatgg tacttttgcc
    16981 tgattcgtga tgtctaaaga aatctggttg tttttggctc ctggtgacag aacagattca
    17041 tgatcttgtc catccacttg aattgaccca tcttcgtgta taactatagt aactggctct
    17101 tttagacctt ggtatccttc tggtgtaaaa gtttcggtca gcgtataagt tccaggtttt
    17161 aaattttcga atagaaaggt atctgtttct tgtccatctt ttggcgattc taagtccatt
    17221 tctggtccct gcagtctgaa ttttgctcct tttaatgtct gtccgttgtc ttcttttttg
    17281 tgtaccgtta gatcaaacgc ttttagtcga ttttgatgag tgagcgtcca tacattattt
    17341 gtcatctcgg tattttttcc cgtgtaaatg atttttccat atttatcgat ttggaattca
    17401 taatttcccg gcgaagtatc atatccggca ggaccagctg tttcaacgac tcgatattta
    17461 ccaggtgtga gtgaatcaaa actggcaaga cctttttcat tcgttgtttg gctgtcaatt
    17521 ggctgataag taccatttgc ttcttttttc tgtagggaaa atgctgctcc tttaagattt
    17581 atctctttgt ccgtcccttg catcgcatac ttacgtactg ctacaggaac ttcaacaaaa
    17641 ggattttcaa tagtcaactg gatcgtatca tcggaagtag tgactgtttt tccatcaatg
    17701 gttaccgtac catcagaagc gatcttgatt tcccaagtag tctttttgct taatccatgc
    17761 ccttctggag cttttgtttc tgtcagcgta taggtcattt ctttttgcaa tttgacattt
    17821 tctggaagag aataggttcc gtcgccatga tctgttagta aagtatcaat agaatctcct
    17881 gttaatttga aaacagcgcc aataagatcc ttttcacctg tagaggaatt tttcgttatt
    17941 ttcaagttta acggtgtgaa ttgtttagta tttttccatg tcattgcatc tatttgttga
    18001 gaatcgtatc ctggtacagg taattctttg attgtttggt aactgaatgc ttgaccttga
    18061 ttattgtatt gaggtagtga taatatctct tgataacttt ctccgctatt tgcagataat
    18121 ttgtcaatgt ctgcacgttc ccacgtattt gttgtatctt tagctggttt aatgattcga
    18181 atatatccgt ttttccaagc tgcagcattt gtcgtatgtt cccgttgaat ctcaaaagta
    18241 acttgatctg gacgatcagc tagattgttg tcaaattctt cccataactt tttgatgtga
    18301 agactgactc cgggagcttt agcagatggt ataccgaatt cagctaattc attggtatca
    18361 atgtttggct ggaaagttgt cctgccgttc atttgatacc agaaatttgg atggaagtcc
    18421 tcattttctg tttggattct cacttggtaa tggatttgga tttcttggtc ttttcctaaa
    18481 tagatctgat tgctcttgat ggtatttcct tctatggaaa tagatggagt gaccgttgta
    18541 ggacttgtcc ccacactctt gactgaaagt gtaccaggct gataaatgaa tggttctgcg
    18601 attggatcat ttatttgtcc attgcttaca gttgctgaga tctgtacagc ttttttggct
    18661 aggtattcag agatatctgt tgcatgatca gctgattcat agtaaagatc gcctttttca
    18721 tctgatgaaa ccatttgtcg catacgagac tctacttctg cttttgagag accagctgcc
    18781 ggatcgcttt gaagttggat gccaagacca tgtatttcga ttcctcgttc tttgagtgcc
    18841 atcgcttctc cgatggttgc gataaacgta ctgtcgattc ttctggatag attgttttgg
    18901 tcaggtgcat catagattct tgagattaga gaagtatttc ccggccgatc ttgcgtatta
    18961 gaaaactgag ttccgtaata attgctgctt gattgtgcgt gtacccgctg tactttatag
    19021 gaaaatgttg gtacaccatc cgtcagcaaa acgatcactt ttttatgtcc atttggaacg
    19081 gatagcatgc ttcctgcatc tcttagtgct ttttgtgtaa aagtaccacc atttgtccgt
    19141 gaaggtgtaa tggattttac ttgatttttc actgaatcaa atgaacccat ctgtactgca
    19201 ccgttactgt agctatatcc ttcgcttgag tagccgacat atcccatatt gattttgtct
    19261 gtgataccgc tatctgctaa agtatcgaca aaacgatcga caccaatctt tacttcaccg
    19321 atccgattat tgtcgttcat acttcctgac cagtctacga ccaatacgag atcaagaggc
    19381 gtgatatctt tttgtacatt tccacgagca ttcaaataaa catcaaacaa tccgggtgta
    19441 cttgtttcct ttgcatactt tcgtatcgca tagtctgctt gattaccggt tcctccatat
    19501 tcgatatacg aatgggtccg atcattagga tctccattcc aagaatttat accatcccat
    19561 tgatttgttc cgtttttatt gccttgatga tcaagaacat ttgtatttcc tgtaggttgc
    19621 caactggctt taggataagt gccctgatca tctgttgtat attctgtagg aatcgtttga
    19681 ttagatggca cactcacagc tggcaaggat aatgtaggca atgtaaatgg gctgacagaa
    19741 ttttcgtttt cttttgatga ttcttcttct atttcgtctg gctttggtac tgacacatct
    19801 gtatctttct tattttctgt ctcatcaata atgtcatatt ctttattatc gacgatcagc
    19861 ttgtttgcct ggctgtggta aagtgctgga tcgattggaa aactcagatc gatagtttgt
    19921 ttagtgtttg atgagatttc caacgtaatt ttcccgctat ttacctgata agcatagcca
    19981 tctccttctt ttctttccac atcacttaag taattttcga ctagaccaat ttctttttct
    20041 atcttttcag cactggtgtt attcgcattt agcttgatgt tcagtgcttt ttttccagac
    20101 tgatccgaaa tgacttcagc tgaaattgtt tctaaaactg ttggatcatc tgtagtgtct
    20161 gctattgcag ataatggcac aaagtttgat aaactcaata ttatcagcat gaaaataact
    20221 ttcagtttct tccctgtggt cgtcattttt cccctccttt ttcttttttt ctgttttgac
    20281 gtgaatagtg aaaattatta aaataaacag gcgttacata attttattta ttttaatatg
    20341 ataattatta tacgaagggc gctaaaggga atcacaccaa ttttcatacc aattggtttc
    20401 atttatgaag ttgattcctt cgattaatga gcgtttcatt gaaagaaaag ggttgaggga
    20461 gaaaaacata tgatcaggtt gttggaatga aactatagaa agatggaaaa aatcattcga
    20521 attctgtcca tttctgacaa gtggatgact aaaaaagaac ttgccaaacg aattggtagt
    20581 tcagaatcta catttgtaag gtatatcgaa gagatcaaaa tccgctgggg aacggatgta
    20641 acgattcaat cttcacaaaa attggggtat cgtcttgaac atttgaatgt accagcttat
    20701 ttaaatgttc tgatggaaat gtcgcaaagt tctacaaaca gtcgtttatt aaatgagatt
    20761 attgtcaatc cgggtaaaac gattgagtat tattgtgaaa aaatttccat cagccggtct
    20821 tcttttacac gtaagttgaa acaatgcaat caaattttag aaagctattt attaaaagtg
    20881 atcgttgacc aaggttttca gttaactagc cttaaaagtg agctccagtt gcgtatcttt
    20941 gttacctttt tctttttaag ttactacgga agacgcacgt tgccatttcc aatcgaaaaa
    21001 aagaagatca aagaaattat ggtcaagaat aattgtcaga tgaatttgtt tcaggaagaa
    21061 agcccatatg agcaatcttt ctttattatg tattttgttg tgcatctcat gagagaatcc
    21121 caaggaactc catttgaatg gaaagctgga gactcttatg tacagttgat aaatatcgat
    21181 aaaaaagact tcttaatctt gaaaaatctc ttaggaaaat tgaagtacag cagctatata
    21241 aaagtagtga actattttgt caatacgatt ttttcagaat tttcggagaa agaaaaaaat
    21301 gaaatcagtg agcagatacg aaaaaatatg tcctactgtt cgttttcatc cgctgattgc
    21361 aaagaaaaaa acgaagaata tgttgttcaa ttattaacgc aaatttattt tttctcccgt
    21421 attttcccat acgatacgac tcatttgacg atccgtgcaa cttactttgc acagcagatg
    21481 caacaccgcc agatacgtct atatgctgga tttcgtgcag aacttcaatt ttattccact
    21541 ctactgaatc agaatctgat ttattattta tctagctata tcttttggat gcttatcggt
    21601 aatcctgaaa ttcaccatta tacttctgat aaaaaaatac tgattatcag cgacttaagt
    21661 cttcgacata gtcaatatat tgaagaatat atcagtgata ttcttgctgt gcataaaatt
    21721 cactctgaga caactgctat tactgaagat cagctgtcga aatacaatct tctcgagtat
    21781 gatctgatcg ttacgaatca acccattttg aatgcccatg ttcctcatat attgattgat
    21841 gatagtgtgt cattcgcaaa tgaagaagaa ctcatccgct tattcgagtt atgaataatc
    21901 aaaaaacagc tatgtataaa ccaccctgat aaaaatttca ggacaaatac acatagctgt
    21961 ttctttttat tgtattcctt cagcaatcca acgagattta tagcgaaagt cttttaacaa
    22021 ctcttctttt gtccaattat ttgcaatgag atccgtcaaa gtgttcatta cgagggtcat
    22081 ggaaaagtat aggaaactaa ctgcttcttc cgacgattga atggagaaat gattgattct
    22141 taataattcc agccattttt cgttcattgg agaatcagcc gatagttctc tacgtttggt
    22201 tgtctgctga tcatttcgct gggttaacaa gtataacctt tgccaataga tactttcgtg
    22261 atctaagtcg cagcaccact ctaaatattt ttcgatcccc aaaaagaaat gttgtttgtt
    22321 ttcgctaatg aattttaaga tatcttgatg gactatgtga gaactgcttt tgatcgtata
    22381 caaataagca tcttccaaat cttgaaaata tttataaaaa gcaccgcgcg acatccccat
    22441 cttgtcaacg atttctgaga ctttgacttg gctgatgtgc tgtgagcaaa atatctccag
    22501 taataggtca tcgattctct gtttctttgc gttcgataaa ttgaaaaaag tttcttttgg
    22561 catccacatc tctcctttat ctctgattat acgtggatag cccttcattg tcatgaagga
    22621 ttcattcctt ctttttctct tcttctgtca agtacccatc ttccattcga taaacgcgat
    22681 cactccattg aatcatacgg acatcatgag taaccataat agtcgctttt tgtttctcat
    22741 gagcttcttt aactagtaat ttgacgactt catatgcatg atctgtatcc aaactagctg
    22801 ttggttcgtc tgctaagatc aagcttggct cattatataa agcgcgagca attgccactc
    22861 tctgtctttc tccaccagaa aggtctttag gataactatt tttaagtgac tggatatcta
    22921 aagattgcag caaggaatcg atttttgttg aatcttcttt ttttgaagta acttggtcaa
    22981 ctagttcaaa ttgttccttt accgttaaaa aaggaatcaa attagagctt tggagaataa
    23041 atcctatttc tttaaagcgt aaatctgctc gttttttttc tggaagctca gttagtgagt
    23101 gaccgttgat cactatttct ccggaagtcg gtgtttgcaa tcctcctgaa atggtaagga
    23161 aagtactttt acctgaacca gaaggaccaa tgatgctgac gaattctcct tcctttacat
    23221 aaaaatcaat tgctttcaaa gctgagacct ttgtatgacc agaaccaaat gttttggtaa
    23281 cttttttcat cgttaatatt tttttcattt ttattaacct cctattgctg taattgggtc
    23341 tactttggtt atcgtacgta tcgaaaacaa tccgcctagc attgcgacaa tgatcaaaat
    23401 cccactatat agcaaccatt gtgaccaaaa aacagcaaaa ggcatcgcgc ttggtaaaat
    23461 cagactagtc aaataagcaa acaaaagagc aagcagaacg cctaccaccc caacaataaa
    23521 ggactgtgct actaaagatt tagcgatgaa gaaatttttg atcccttgtg ctttcattac
    23581 tccaaaaata gcagtcttct gaagtgtcat cacgtacatg aaaataccga ccacggcagc
    23641 aaccactaag aacaaaaagt atatcatcgc atttaaggtc atattttggg cagaatagcc
    23701 aggaatactt tcaatgaatg tcccaatagt taataactgt agacccttcg atgctttttc
    23761 tttcgaaatt ttagctgaat cctttattac gatcccattg atcggctgtt cttcttcaga
    23821 agcaaacggc tgttgtccat acttcaaagc agtccacgta tctagggacg catagatgac
    23881 gggagaaact gtgtaataag tttcaggaaa gataccgaca atcgtcaaag gatcgtcgta
    23941 actgccaatt ttgatcgtat ctccaatttt atagcctgaa tcagccagat tttgagagat
    24001 gatgatttca ttctttgttt taaattcatg ccctttcgtg agttttggta ataaaaatgc
    24061 ttctttcgtt gtgccgaaaa ctgtaacatt ttctttgttt tttcctttga ttgctccgct
    24121 gtacaatccg attggtgctt tctctcctcc ttcaatgtat ttcagatctc ctcttgttaa
    24181 ttgtgaagct gcaaatactt gatttgcatc ctccgacaaa acaatctctt gtgcgtccca
    24241 atcatcgatt gcttttttaa actcttctga aagtccattc gctagtcctg acaacatgaa
    24301 tacgacatac gcaatcaaga acataatacc gacgatcaag ccatatcgta atttagaata
    24361 gcgaatttct ttgatagcta gaaacatttg tttttcctcc ctatgataga ttgatagtga
    24421 caaaatgtca cttgttgaat agtgtaactc taaagtgaca atttgtcact tgtgttgttt
    24481 ttttcttatc aataaattgt ctaagacatc tttcgataaa accttaacat gagtcataaa
    24541 aaatttattt aagccttatt tatgctttcc atgataaact aaagtgacca aatcaatgag
    24601 gtgggaaaat gattaaacta ttagtagtag aagacgataa agtattatcc aataatataa
    24661 aagaaatact cgctgcaatc ggcgagataa cacaagttta tgatggtagt gaaggactgt
    24721 tcgagttgga aagtggagtt tatgatcttg caatcttgga tttgatgatg ccggaaatga
    24781 atggatatga agtgttagca aaaatacgga aagaaaaaat acagactcca gtattgattt
    24841 tgactgcaaa agatagtttg gaagataaga ttgaaggatt tgaacgaggt gcagatgatt
    24901 atctaacaaa acctttctat cgagaagaac tgatcatgcg cgtgaaagca ttattgaaac
    24961 gctcgctagg tctctttgat gaaaatgttc tgaagtataa ggatatcagt tgtgatttaa
    25021 aaagcaatga agtcttgtat aaagaacaag ttttgcccat acaaggaaaa gagtttgaat
    25081 tgctcgttta ttttttacaa aataagggaa tcattttgac aaaagagcag atatttgacc
    25141 ggatctgggg atttgattct gatacgacga ttacagttat tgaagtttat atgagcaact
    25201 tacgaaaaca tctgcgaagt actggattgg ataaagaaat caaaacctta cgtaatgtcg
    25261 gttatatact gcaaagggtg tgatgcaatg aatggagata ttacacgaaa gcaacgtagt
    25321 cgcttttttg cgacaaatgt agcagcattt gcttttatct tttttctttt gggattcatt
    25381 actttacaga tattaaacca atctgcatac agagaaacag atctgtcatt agagaaaata
    25441 aattttgatt ctcgaacagt acaattagag atacaaagat atcagcaagg cgatcccttt
    25501 ttaaacccga ctcaaacgga acaaaacaga acaaacatgg aacagccatt aatggaacca
    25561 tcaaatgacc gttttaacac tcaagtgatc ttgtggtcta aaaatggtga gatattgaat
    25621 aaagcagcta ttggtgggcg tttcaaccaa atcaaagaat taaaaatgga tacgaaaaat
    25681 ctgaatacca tccaaaatat tgaattaaat gaagacagtg gctcatctgc cctctctttc
    25741 cgttcgatta cgaaaaaagc ggagacgaat caagatggcg ttgcctacat ccaaatttta
    25801 gaaaatacaa atcaaatcaa gaattcatta gcgacttttc gcacgattat gattttgtgt
    25861 atgattgttt tttggattct ctctattggt atcagttatt acttgtctag tctaagcatg
    25921 cgccctatta ttctttcttg gagaagacaa aaagaatttg ttgagaatgc ttctcatgaa
    25981 ttgcgcacac cgttaacgat tattcaaaat agtttggaac atctttttac caagcctaat
    26041 catactatta ttgaagaatc cgaaagcatc gctcaagcat tgtcagagac gaggcgcctc
    26101 actggattga cttcggattt gctgacaatc gctcggaatg attctgatca gcagctgctt
    26161 tcaaagcaaa tgatcaatac acaagaatat atcgaacaac ttgttaaacc atttcaagag
    26221 atggccatca tagatggaaa agagtttatt ctccataacg atgcaacatt agaagtacca
    26281 attgatgaaa aaaaaatcca tcaagttttg gttattttgc tggataatgc attaaaatat
    26341 acgaatccag aagataagat cattttggat tcatctgtta accataaaga atggctgatt
    26401 cacgtaaaga atactggccc ttctatcagt gaagaagatc aagcaaggat ttttgatcga
    26461 ttttatcggg aagatcaatc tcgggcgaaa gagactggcg gatacgggct tggcttggca
    26521 attgcaaaac aggtaatcga acagcatcat ggacagattt ctgtatccaa ttatcttcca
    26581 caaggagtcg attttaaagt ccaattgccg ttgaagccta aaaaataatg cttatcccta
    26641 aaaaagggct gtgacaaaaa tcttgtcaca gccctttttt gttgcgaata aacggtgttc
    26701 cagaagtagc ttcttcggaa ataagccgaa atttctttaa gctgaccact tctgtcacaa
    26761 cctctttagc ttacgcttac gattttttat tgcggtcttc aatgtgtaca tcaccgcttg
    26821 tgtcaatatc caattcttct cggctaacat cttctgtcac tttttcaaca tcttcatgtt
    26881 cttctttatg aatcccaact tcttcacgaa taactgtatg tttgcttaca tctacttgct
    26941 cttctttgat tgggatagta atcgtgtcat cttccaattt ttcgtctgtt ccctgtgaag
    27001 attgatctgt tactggtttg cgttcgatga cgatttcttc acgtttgacc ggtacttcta
    27061 ccgtttcagt atcgttgacc acatgtttgt ggatgtcgac ttctccagtt gtcacatcat
    27121 gtgtatttac atctaatttt tcttctttta gttgaattgt atcatcatct agatttggtt
    27181 tgcgatcgat gtcttcagtc ttgcttgtac ccattgtatc agtctcgcca ggtacgtcca
    27241 tcggatcagt tcctggtgtc atattcaatg gatccgttgt attttgaaag cctgtcgtgc
    27301 cacctacgtc cattggatct gttccagtgc cagtttctaa tccagtcccc attgtgcttg
    27361 ctgggctaac agtatcttgt gtatccattg tatcttctgt tgtttctggc tgacgatagc
    27421 cttttactgt gatgacaaat tttccatcag aaatatctcc tctatattga tataaaggat
    27481 cattttctgg tgtagccgtc tcagaatcat aggtatcaaa agaaaaagta tcttttattt
    27541 tttcccataa agaacggtct tcttcaccat ccatccgatt ttcatctgtt tcaacatcta
    27601 taccagacaa tccgtcaaat cctagtctag atgctgcttc atcagtagta taaataacga
    27661 tatcatctcg ttcataccct tcgttacgca accgttcaat tactgataat gcttcttccc
    27721 tcgtactata gcttccgatg accgttaaat ttttctcttc catgtaaatt cctcctagca
    27781 aatagattta tgattggtat attgttactt ataaatttat tctataggag acgattgttt
    27841 tctacaaata tttagattgg aatttgatta aaacaattta attgaaattt gaaaacattt
    27901 atgttaatga aagtcatatt ctaataaaat gtcatttgtt tctatatgaa tttttatgaa
    27961 aaatttcata taatttccac tcgttgtgat ggtttaattt atccaatcca tcaataagca
    28021 aacaacttat ataaaaaagg attaattctc aatataattg ctcctaccat agaataaatc
    28081 ttcatgtaaa gtaatgaagt accaactatt tctaatactt ctttaagaat tatcccaaca
    28141 atatcgttag acaaaccatt attgaataaa tggagagcca atcgattctg aaaaaatgtt
    28201 ttctaatttt tcggaaataa aagaaagagc ctcctgccat tagaacgaac caaatccatt
    28261 tcattgctca tgaagtaacc acatagctaa aaagaggata aatcaattaa aagatcaaaa
    28321 gaatgactgc actaataaaa taactaaaaa tgtatcttct tacttcatat cttttagcta
    28381 gtacttagtt aaaaatttgc ttcctaagat caagacaaac tgtctatctt catttgatga
    28441 aatgcggtaa aatatagaaa atgaacacta ttggagggaa tttgtaatgg acagcaaaac
    28501 tagaaaacaa ctgcaagaaa agttaaagca acgtacacac ttgctccctt ccgtctttca
    28561 acagttttta gaagaaaatc gttcttccct ttcattaggc agtcgctatg aatacgcaaa
    28621 agattttcat ctgtttactg attatttgag aaaagtattt tctgaagaag tggatgatca
    28681 gctgattatc catttagaaa aacaagatta tcagaatttc ttagctaaaa tctatcgaca
    28741 tactcgttcg ttccaaacta cagcgaacca agaaacggag caattgtttg aaaatagcta
    28801 ttacgggatt tcaaggaagc aatattcatt aaatcatttt ttacgatttt tgtatcaaaa
    28861 aggactgatt gaagaagaga tttctctttt gggtacaagt ttaccggaaa caaccaaagc
    28921 agctccgcga tatatcgatg cagaactttt caaagatttt gagcatttgt ttatcccgat
    28981 tgaaggcttt caaacaagca gagaagctag ctttgaagaa aagaatagac cgaggaacct
    29041 tgcaattact gccattcttc tgtattgcgg attaaagatc catgaagtag tagaacttaa
    29101 acgtaaagat gtatctttga ccaagcagat tcttaatctg aatagaaaag aaaacagact
    29161 gaataaagta ccaataccaa aagaagcgat gccatttctg gagaactatt ttaagttaac
    29221 agcacaaaat acggacgaaa attcttttgt ttttcgttcc tcacatgatc agcaaatttc
    29281 tcctcggact attcgccaaa ttttgagcaa aattacagta tataaaaacg tatcgccaga
    29341 actatgcaga aaaacatatc gttattatgc agaactatat ttagatgatg ctgacgcggt
    29401 aaattattta tgtggtaatc gatacctctc atcccattct tttcaagata tctgggaaaa
    29461 gatggttgat ttttcttatc acgaattagc ggcattcgtt cgggtttctt ccatataaaa
    29521 caaatgtatg atcttttttt cacaataaac tatgttatta tataaaggat tatgttacga
    29581 ttatcttgaa tttgtatatt acttaacaaa agataatgga gtgtgaatga tggagaccat
    29641 aacgtattcc cctttgaact tatttacgaa ttttcaagaa gctgcagcta acttttccga
    29701 gaccccgatt atctttgatg agccactttc agctttccca gaattaggac ttgaaacaac
    29761 ttacaaaaaa tgctgcgaaa gtgtgttgaa acgggcttat caattggctc atcttggagt
    29821 gaaatccgga gacaaagtta ttatttataa gagttccgct tttgacacat accttctagc
    29881 tgtttctgtg tcttaccttg gtgcagtgcc tgtcatgact tcttatcatc taccaacaac
    29941 aacaatggaa gtatttatcg atcgtttaga agaccctttc attttatttg atgatgagac
    30001 aaaaaagcga gtgagagaaa tttcaaatgg aacaaaatca aaacagattc ctattcttta
    30061 tttaatggaa caacctgctt tgtctgtttc tcaatcattt ttagacaaaa atgaaatttc
    30121 ttatatgacc catacttctg gtacaactgg tatccctaaa cttatttgtc attctgctca
    30181 ttctatggga tggcggacga aatggcaaaa aacagttttc acaaaaattt cggaaaagaa
    30241 attaatcgct tttcacatct ctccagtgca ttccagattc aatattggta tctcttcttt
    30301 gatgtccatg ggatttccta tgatgccttt agcaaacgca caaagttcaa aagttgttca
    30361 tatgttggaa gctcaccggc caattgcact agagacccat ccgaataact ttgttcagtg
    30421 gcgttttact gctaaggagc atccagaagc tttttcgggt atccgatact atcactccac
    30481 ttttgatgct ataaacaacc agacaatgaa gacatttttg cgaacgtcga tggcacagga
    30541 tgcggtattt cttcaagtat atggccaaag tgaatgcgga ccaatgattc ttaaggctca
    30601 tacactagag agtttgaaaa caagtgatgc aagagatatg ggcgttggac tggaagacat
    30661 gacaagcgct cggattacag attcagttgg gaatgtccta cctgaaaaca cagatggtca
    30721 tatacaatta ctgtctaaag gacgggcttt gacttattac aaggaagatg cacggttcca
    30781 ggaaaatgtt tatggcgatt ggtgggatag cggagattac ggttttatgg ataagcatgg
    30841 ccacctattt ttgaaagatc ggcaagtgga tttgatcgaa acgatcaata gtacattagc
    30901 tattgaagac tttttattag attcgttaga ttttttggca gaagtagtga ttgtaaggga
    30961 taaaaatcat tctccacaac cgattattgc tttagctcct gataaagaaa tggattggaa
    31021 tcgatggtgg gagcaagtcc atgaacttcc tcgcttgaac gtccctatca tccgtgattt
    31081 tgatgcaatc ccgcgaacag ctacaatgaa agtgcaacgg cttcaaatcg agaaagaatt
    31141 aaaatcacaa aagtcctaac tatttaagga aagaaagatc cattttggtc tttcttttct
    31201 ttttgttttt ttaaattgga tgagataaaa atatgccaat tggctgtttt tcttttttga
    31261 aaatttcgtt atactcttat tcatggagga aaatgttatg aaaagaatct tgacaattgg
    31321 cggttctgat ccatttgctg gtggaggaat ccaaacagat ttaaaaactt ttgagaattt
    31381 tggtctattc ggactaagcg ccttgacttc tataggggca ctagatatta atgagcgatt
    31441 tatgctggaa tctatatctt tggaattgct taaacgacag ttagtttcga ttgataaaat
    31501 gacttcatta gatggtatca aaattgggtt attgaattca agtgaagcaa tcatggttgt
    31561 tcgagacttc ataaaaacaa aacaaaatat cccaatcatt ttagaccctg ttttggcatt
    31621 caaagaaact gaagctgtga tcaatcaatt gtatatttcg cagctcatcg aagaactttt
    31681 tccacttgtt acgatgatta cgcctaattt ggcagaagct gctttacttt ctggtcaagc
    31741 tcttccctct tcgttaacag aaatgatcca tctcgctaaa gaactcactg cttcaggaac
    31801 aaaaaacatt gtgatcaaag gcggcacaag gatggctgga gatgaagctg tggatcttct
    31861 ctattcaatt gatcattatg aggtattttc tcataaaaaa atcgacacta caacagtaaa
    31921 cggggccggt tgtgcctttt cttctgcaat aacagcgaat cttgtgttag gggcagattt
    31981 taccaaagca gtagcagaaa gtaaaaaatt tgttgcccat tgtatttcta atggcgtgct
    32041 gatgaacgat cataccggaa gtgtctggtc tggcggtatt caaaaaggag gaatgagaaa
    32101 tgaaaactaa gcaattaacg atctatgcta tgctgattgc gttgacagtt gccctttcat
    32161 taacaatttt gatccctgta ccggctacga atggatttgt gacattatgc gaggccggga
    32221 tctataccgc tgcttttctt tatggtccaa cgggtggatt agttgttggt gcttccagtg
    32281 gtctattgat cgatttgatt tcaggatatc cgcagtgggc agttttttca ttagtgatcc
    32341 acgggttaca aggatatgtt gctggttctt tcagcaaatc ttctactcgt atgtggggat
    32401 tagggctttt attaggaagc ttagttatga tcatcggcta tggtatcgcc ggttggattt
    32461 tatatgattg gccggctgga gtggcttcta taccaggaaa catcttccaa aatctgattg
    32521 gaatgggact tgcctttcct ataactgcaa gtcttcaacg tattcaagca cggaagcaat
    32581 attaattgaa aaagaaggtt ttagaaaatg aagttgtcag aaaaagaatt aaaagaccaa
    32641 ttgactgaaa tagttaatga catattggct gaggcacact tgaaaaaagg tgatcttttt
    32701 gttttaggat gcagcacaag cgaagtcgtt ggaggacata ttggaaaaaa ttccagtgcg
    32761 gaagttggtc aatggatcat ccgtacgtta aaagagttgt tggatcctaa agaaattgcg
    32821 ctagctgtcc aaggttgtga acatttgaac cgagcattag tagtagaacg aacagtggca
    32881 gaagcaaaaa atttcgaaat tgtctctgtt gttcctgcac tacatgctgg aggtgcttgt
    32941 tctgttgctg cttttgacca gttcaatgat cctgtcgaag tggaacacgt agttggacaa
    33001 gccggtatcg atatcggtga cacatcaatc ggtatgcata tcaagcatgt ccaagtacct
    33061 gtcagacctc gtttgaaaac ccttggacaa gctcacgtga cagcccttcg ctctcgtcct
    33121 aaatatatcg gtggtcctag agcgaattat tgaaatgagt aattgacaga gcaatctgaa
    33181 aaacttcctc gcaaagtcca actaatgaac tttaacgagg aagttttact tgtttgtttt
    33241 atataaccga gcttcataag gtgctagtat gcctttagct tcaaattctc cgctagagga
    33301 atttgacaag agtaatgtcc aggactgatc tgcttctttt ggaagttcat agtcagcaag
    33361 tgattctgtt aagtttacca taataagata ttgttcatct tctaaatatc tttcatagac
    33421 ataaagttgt ggatgttcat gaagatattc tttataagaa ccgtatatca gacctttatt
    33481 ggtttgtcta atgcggatca tgttcttata gaattgcagg accgactctg gttcatattc
    33541 ttgttgttct acattgatcg ttttggtatt tggattagtg atcagccaag gttcatgtgt
    33601 agaaaaacca gcatattgtt ccgaagtcca ttgcatgggt gtcctgctgt tgtctctcgt
    33661 tgtttcacga atgatatcca atgcttcttc tcttgtctta ccttccgcaa gcagcctttt
    33721 ataaagtctt ttcgaatcta ctgcatcaac ttgatcgatc gattcaaagg gcatattcgt
    33781 cattccgatt tcctgtcctt gataaataaa tggcgtacct tgcaaaaaga aatacatcat
    33841 agcgattgct tttgcagaca tagcccaaaa atcgggacga gtatctccaa acactgaaac
    33901 ggcacgagga acatcatggt tttccatata taaagcgttc catccgatac catccaatga
    33961 agtttgccaa gcgcttaatg catgtttcaa tttaaggaca tctaattgcc cttctttttc
    34021 ttgctgccag atatggatat gatcaaattc gaatatcata tcgaaataac cgtctttccc
    34081 tacccattgt ggtccttctt ctgcagtgac accacttgct tctccgacag tcatgatatc
    34141 gtactcttca aaaacatgtt tcaaatcggt caaatgttct tcgattcctg agacattttg
    34201 aaagggtgag aaagggttct ctgtggcttt tactgaatat tcatcttttt ttacatgaga
    34261 aatcgcatct aaacgaaatc catctatccc tttatccaac caccatcgga tcatattgaa
    34321 tagttcttct ttcaatttct cgctttccca atttaagtct ggttgttctt ttgcaaaaac
    34381 atgcagataa tactgtccag tttcatgaga atactcccaa gcagaaccgc cgaaaatcga
    34441 tgtccaatta ttcggttctt tgccatctac cccgtcgacc caaatgtaat aatcgcgata
    34501 cgggctttct ttagattttt tcgattcttc aaaccattga tgctgatccg aactatggtt
    34561 gatgacaaga tccataatca ctttgatccc taattcatgt gcttctttta gcaataaatc
    34621 aaaatcttcc atcgttccga atctctcttc tatatcttga tagtcactta cgtcataacc
    34681 attatcgaca aatggagatt gatagatcgg attgatccag ataaagccga taccaagatc
    34741 tttcaaataa ttaagttttt cacggatccc attgatgtct ccttgaccat cgccattgct
    34801 atctttgaaa ctagcaggat agatttgata gcctactgct tctttccacc atttttgttc
    34861 ttgtatcata tcgtttcact cctttaacgg tccctcttga ttataaccga ttctatttta
    34921 gaagtatcct tgttaccggt aactagaaac gaaaagctca acgaatctgc taagtgcgtt
    34981 cgctgagctt tattttatca tagatagcgg gcaatcagtt gaatcatttg cttgggaaat
    35041 tcattcaagt cagtaatatc tacaaaacga ctttcaccgt agatcgactt gatttcttct
    35101 ttgtcttggc caatagctgc ggagacaaat aggattcctt gacgttcata ttctttcaaa
    35161 acagactgga tatcttcttt tgcttttttg cctgtataat ccggcaacgc ttttggctga
    35221 ccgtcactaa tattaagcaa cagtttggtt gttgcggatt gtctgtttaa tttttctgct
    35281 gctaaacgaa gtgcagcacc atcacgattg ttttgccttg gtttcatagt gactaatttt
    35341 tcatttaacc attgaaatcc gtcttcaaat tctttataag aaaacaaaga agtcttttct
    35401 cttgacgaac ggtctgctgt atctccgtaa atcaacaatg ggatatcgac tttcttagaa
    35461 aattcggcaa tggcaatcgc tgcttttttt gccgcttcga tccgatcatc ccgtatcatt
    35521 gacccagatt catcgatccg tacagctacc gctaaagaag gttgctcatg tggcgggttc
    35581 tttttgtcaa aatttctcaa atcaccatat gctactttgc ttgcattgaa ccgctgacct
    35641 tcgtattttc ctttttggta cgtctctgtt tgttcattat ccagtaactc taaaatttgc
    35701 cggctaagtg attctactat tggcattact tgcatgtgta gctcacgtgc ttccggcgtt
    35761 tttccttgga aagctggtcg gtgaacgatg agaccttctt tttcatgaat cgtttccttc
    35821 atgatttgtc gcgcttcttt attcagttgc ttcttgattt ccttatcatg tgcttccatg
    35881 tcagaagcag acatttcttg agaatcatct cctgtttgtt gttctttttg attttttcct
    35941 tcgatatcgc tactgcgttc tttttgcatt tgaggaggaa tctcgttttt ttctattgtt
    36001 tcttcttgtt tctcttctcc ctcatttggt tcttcagcag acaacgattt cttttcatct
    36061 aagcgcagaa tttcttcagc attttcagtg atgtttcgtt catttgcttc ttcggcgcta
    36121 atggttcttt cttttgtatg ctcagattga ggatgacccc atttttcttt tctcaatatg
    36181 tcctgaagtt ccagtagcaa tccactgtca gacaaagctt gttcaagaat tgccaattca
    36241 tctgaatcag tggataattt atacaaaact tttggataaa tcgactggag taaatcttgt
    36301 ccttgcttat aggtatttgc ccaataaaaa taagaacgca ttcctgctac acctttgatg
    36361 gcgttagctt ttgcagtttc atccaacaaa cggataatct ctgccatctt taagagaagt
    36421 tcttgttttt ggatcatagt tttcgccatc gcacgttctg ccatgacttc taaggcagga
    36481 agatccattt tttccgcatg ttgcatccga tctcttaaag attcatttaa tgggcgattt
    36541 ccttggtaat ttcgatttgt agtgatcaca atgacgcaat ctggatggcg acgaatgatt
    36601 cctgtcggta aattcaacat cccatttggt tcgagcgctg agttaagtgc cacaagaaca
    36661 gaggcatctc ggatgacagt cggctcttga atctctaata aatagccttt ttcaatcgct
    36721 cgaacgattt cagaagggta atagctgtaa tggattgcat ctggataatc agcttcgatc
    36781 ctttgaacta gctctgctaa tttttcttta gcagaaaaag gatcaatgcc gtaatggttc
    36841 tggatcagtg tcagtacagc ttcaagtgta tctgtttggt aaatagcttc tagtaattct
    36901 tggtcttgtt ccttattaga atctactaca ggtaataagg ctccgaaaac atctgatttg
    36961 tccatatctg caaaacaggt cacttttgta tagggaagtt gcagatcagc agatagtgct
    37021 ttggctaatt gtgtcttccc cgacccggca tctccttcca aaagtacgtt actgattttc
    37081 atttcaggat cttgccaatt ccctttgatt tcggaggcga tccgccattc ttctgcactt
    37141 gtccgatggc ttttcggttt cttccagatc atttgctttt ctagccgtga aaaggttcgg
    37201 ttttctgtta gtgagaaatt ctccatgctt ctcttcccct tttgatcatc tctgtctatt
    37261 aatatcctag ctcatctaaa agacgtttta aaatacggaa tcgttcccca atcagttctc
    37321 cgtctttttg tagtgtatcg taatacaaaa tgatgtcttc atcgatcttt ggatttttgg
    37381 tatctacttc taccgtcatt cggttccctt gtaatccaat gatgtctacg actggatttt
    37441 ctggatcata aaatttttca taacgataag catcttggaa gtataaactt ccttgcgcag
    37501 ctaagatcgc taaatctaaa atacgtgtga ttggtaattc ttccgactgt cgtgaccatt
    37561 tttctccagt atgtcgccat accttgcctg agatatcgac ttttccacga tcattccact
    37621 gagcaaggcc cagtgaaagc ccttttgcat ccatatttaa tgcatttctt ccatcaactt
    37681 tatcatagtc ttctacaacg attactggtt tatgttttaa atttgttggt atttccatta
    37741 ttttaactcc tcatcctttt atttttagta aataagtaaa ttagtagttt actcatttac
    37801 taaactatat gcgacattat agaggattga agtgaggatt gcaaagaaaa agagcttcat
    37861 tgatagaaat aaaaacacca tctcgttctt ttacacgctc attagtaaca tgtagaaagt
    37921 ccaagatgat gttggaat
//
LOCUS       DACECR010000015        37509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.015, whole genome shotgun sequence.
ACCESSION   DACECR010000015 DACECR010000000
VERSION     DACECR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.015"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            333..1217
                     /locus_tag="IWO17_000726"
     CDS             333..1217
                     /locus_tag="IWO17_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0289623.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            1426..2004
                     /gene="pgsA"
                     /locus_tag="IWO17_000727"
     CDS             1426..2004
                     /gene="pgsA"
                     /locus_tag="IWO17_000727"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ0289624.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            2244..3485
                     /locus_tag="IWO17_000728"
     CDS             2244..3485
                     /locus_tag="IWO17_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ0289625.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            3594..4643
                     /gene="recA"
                     /locus_tag="IWO17_000729"
     CDS             3594..4643
                     /gene="recA"
                     /locus_tag="IWO17_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ0289626.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            5054..6610
                     /gene="rny"
                     /locus_tag="IWO17_000730"
     CDS             5054..6610
                     /gene="rny"
                     /locus_tag="IWO17_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ0289627.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(6689..7495)
                     /gene="proC"
                     /locus_tag="IWO17_000731"
     CDS             complement(6689..7495)
                     /gene="proC"
                     /locus_tag="IWO17_000731"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ0289628.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            7636..10209
                     /gene="mprF"
                     /locus_tag="IWO17_000732"
     CDS             7636..10209
                     /gene="mprF"
                     /locus_tag="IWO17_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0289629.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            10363..11214
                     /locus_tag="IWO17_000733"
     CDS             10363..11214
                     /locus_tag="IWO17_000733"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ0289630.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            11459..12397
                     /locus_tag="IWO17_000734"
     CDS             11459..12397
                     /locus_tag="IWO17_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ0289631.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            12420..13100
                     /locus_tag="IWO17_000735"
     CDS             12420..13100
                     /locus_tag="IWO17_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0289632.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            13073..13897
                     /locus_tag="IWO17_000736"
     CDS             13073..13897
                     /locus_tag="IWO17_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0289633.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            14064..14882
                     /gene="purR"
                     /locus_tag="IWO17_000737"
     CDS             14064..14882
                     /gene="purR"
                     /locus_tag="IWO17_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ0289634.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            15385..16758
                     /gene="glmU"
                     /locus_tag="IWO17_000738"
     CDS             15385..16758
                     /gene="glmU"
                     /locus_tag="IWO17_000738"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ0289635.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            16852..17823
                     /locus_tag="IWO17_000739"
     CDS             16852..17823
                     /locus_tag="IWO17_000739"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0289636.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      17829..17931
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            17997..18566
                     /gene="thiT"
                     /locus_tag="IWO17_000740"
     CDS             17997..18566
                     /gene="thiT"
                     /locus_tag="IWO17_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ0289637.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(18647..19420)
                     /locus_tag="IWO17_000741"
     CDS             complement(18647..19420)
                     /locus_tag="IWO17_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289638.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            19687..21387
                     /locus_tag="IWO17_000742"
     CDS             19687..21387
                     /locus_tag="IWO17_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ0289639.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            21357..21575
                     /locus_tag="IWO17_000743"
     CDS             21357..21575
                     /locus_tag="IWO17_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289640.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            21595..22485
                     /locus_tag="IWO17_000744"
     CDS             21595..22485
                     /locus_tag="IWO17_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ0289641.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            22503..22787
                     /locus_tag="IWO17_000745"
     CDS             22503..22787
                     /locus_tag="IWO17_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289642.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            23274..23888
                     /gene="gmk"
                     /locus_tag="IWO17_000746"
     CDS             23274..23888
                     /gene="gmk"
                     /locus_tag="IWO17_000746"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ0289643.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            23893..24198
                     /locus_tag="IWO17_000747"
     CDS             23893..24198
                     /locus_tag="IWO17_000747"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0289644.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            24451..26859
                     /gene="priA"
                     /locus_tag="IWO17_000748"
     CDS             24451..26859
                     /gene="priA"
                     /locus_tag="IWO17_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ0289645.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            26874..27365
                     /gene="def"
                     /locus_tag="IWO17_000749"
     CDS             26874..27365
                     /gene="def"
                     /locus_tag="IWO17_000749"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0289646.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            27358..28296
                     /locus_tag="IWO17_000750"
     CDS             27358..28296
                     /locus_tag="IWO17_000750"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ0289647.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            28296..29654
                     /gene="rsmB"
                     /locus_tag="IWO17_000751"
     CDS             28296..29654
                     /gene="rsmB"
                     /locus_tag="IWO17_000751"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ0289648.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            29667..30407
                     /locus_tag="IWO17_000752"
     CDS             29667..30407
                     /locus_tag="IWO17_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ0289649.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            30404..32473
                     /gene="pknB"
                     /locus_tag="IWO17_000753"
     CDS             30404..32473
                     /gene="pknB"
                     /locus_tag="IWO17_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ0289650.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            32638..33537
                     /gene="rsgA"
                     /locus_tag="IWO17_000754"
     CDS             32638..33537
                     /gene="rsgA"
                     /locus_tag="IWO17_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ0289651.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            33550..34200
                     /locus_tag="IWO17_000755"
     CDS             33550..34200
                     /locus_tag="IWO17_000755"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0289652.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            34201..34839
                     /locus_tag="IWO17_000756"
     CDS             34201..34839
                     /locus_tag="IWO17_000756"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ0289653.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            34994..35848
                     /gene="mreC"
                     /locus_tag="IWO17_000757"
     CDS             34994..35848
                     /gene="mreC"
                     /locus_tag="IWO17_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ0289654.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            35852..36361
                     /gene="mreD"
                     /locus_tag="IWO17_000758"
     CDS             35852..36361
                     /gene="mreD"
                     /locus_tag="IWO17_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ0289655.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            36616..>37509
                     /locus_tag="IWO17_000759"
     CDS             36616..>37509
                     /locus_tag="IWO17_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289656.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSA"
BASE COUNT        12338 a         6671 c         8164 g        10336 t
ORIGIN      
        1 tttgacctga gtattagaga aacatacgga aaaatatctc cttatatttt gccggttttt
       61 gagctaatac cgaggaacag ctgattgaac accgtttaag aaagaggtcg tgagcctgcc
      121 gtttaactcc gagtcacaag gaacaagttc aaaaaacagc ttcttatatt tattggatat
      181 tgtgacttgt gaccgaggag ttggcaggag aacaatgttt attcggaaaa aaggagctgt
      241 gacatgactt ttgtcacagc acccaaacat aacgtctttc gttgcaacag cagatgcttc
      301 ggagaatcaa acagactgga gaagatgaac gagtggcttt tgaaaccatc ggagaaaaat
      361 tacggcaagc acgcctgaat aaaaaaatat ctttagatga attacagcag attacaaaaa
      421 ttcaaaaacg ttatttggaa gcaatcgaca atgatgattt tgaccaattg cctggtaaat
      481 tttacgtgcg ggcatttatt cggcaatacg ctgaagctgt aggagaagat ggagaccatt
      541 tagtcgatgt ttttgatggg aaaagcaaat tgacaggatc ggtccctgaa cgtccgcagc
      601 ctgaaacagt aagtacttct agaaaagcaa tgcatcagga agaatcgcat ccaagtcgct
      661 tttggacaag tcttcctgtc attttattag gattggttgc tttggcgatt gtcgttgtcg
      721 tgggctatat gacttggcaa gatcgtcaag ctgatccaat aatcggagac acagcttctt
      781 cgcttaaagt agaaggatca gtcgaggagt cctcttcctc agagcaaagc acgcagacca
      841 aagagtcttc tacagtagaa tcaacaacaa aatcaagtga agaaaagaaa atggcaatag
      901 ctacaggaga agattctggc agtgccatcg cagtaaatat cacagacgct accaagcctg
      961 taacccttga atttactgct gtcaatcgtg tatggttagg tgtgctagta gacaatgcct
     1021 atgtttatca agggacactg gcagcaaatg aaacacagag taccgtgtta ccggaaacag
     1081 caacaaatgc gaccatcacg attggtgcag caagcaatgc aacgatcaaa gcgaatggag
     1141 aatccgtacc agttaatcca ggggaaaaca atcagagtcc aaaaaatgtg aaccttactc
     1201 ttcaatacgc agaatgatcg tataaattag tttacgcatc tgtttacttg taggtataaa
     1261 aaagaaggat cgtgacaaat ctgtgtcgcg gtccttcttc gttaattcaa aaagatcaat
     1321 ttcaataaaa atactccttt gaataaattc atttggaaaa gtaaataata tcggtggaaa
     1381 cttcgctgat agtcacgtag aatagggaag aaaggagtcg atagtgtgaa tttaccaaat
     1441 aaattaactg tacttagaat ttgtatgatc ccgattttca ttttggttgt aggtttacct
     1501 ttagattggg gacaatttaa cgtaggagac accacactgg ctgtcacaca gctagtaggt
     1561 gcagttattt ttgctttagc tagtatcaca gactggttag atggaaaaat tgcccgtgcg
     1621 cgaggccttg tcactaattt tggcaagttt gcggacccgt tggcagataa aatgctagtt
     1681 atgactgctt ttatcatgtt ggttgaccaa ggaaaggccc ctggttgggt agtagcgatc
     1741 attgtctgtc gtgaattagc cgtcacaggc ttgcgtttac tacttgtcga acatggagaa
     1801 gtcatggctg cagcttggcc gggaaaagtc aagacggcta cacaaatggt tgctattatc
     1861 ttgctgttta tcaataatat cccattcagc ctgatcggta ttccagtaga tcaaatcatg
     1921 ctttatgctt gtctgatctt tactatctat tcaggtgtgg attacttcat caaaaacagc
     1981 ggtgtgttca aaggatcaat gtaagtatcg tttagtggag aggttgtgag cctgccgtct
     2041 aactctgagt cacaagggat aatttcaaaa aacagctttt catgttttat gtatattgtg
     2101 acttatgacc gaggttttgg caggagaaca ctgtttattt ggaacaagga gctgtgacaa
     2161 gatttttgtc acagctcctt gccctctttt taaataaaaa aatataaaaa tacagcgtat
     2221 actatataag ggagcgggaa gtaatgaaag cagaaataat agctgtcgga acagaacttt
     2281 tactaggaca agtcgtgaat acgaatgcca ctttcttgtc agaacagcta gctgatctag
     2341 gtatcgaagt ttattatcaa acagttgtag gagataatca gcagcgttta gaagagttga
     2401 ttgctctagc tgaaacaaga agtgaactca tactcttatg tggcggatta ggaccgacag
     2461 aagatgacct gacaaaagaa gcaactgcgg ctcacctcgg taagtctctc atacaaaata
     2521 cggaaggata caaaaaatta ttggcatatt ttgaaacaac ccatcgaaaa atgaccaaaa
     2581 acaatctgca acagtcccag attatcgaag gcggggttcc tctaccaaat cgtacagggc
     2641 ttgctttagg gactttttat caaacagata cacatgcgta tatccttctt cccggtccgc
     2701 ctaatgagtt gaaaccaatg tttgtggaac aagtacgtcc tttactagag gaacgttttc
     2761 cttctgaaga aaaactaatt tctaaagttt tgcgctttta tgggattggt gaatcgcgac
     2821 tggtcactga gctaaaagat ctgattgaga cacagatcaa tccaacgatc gcaccatatg
     2881 ccaaaccaaa tgaagtgaca ttacgactga cagtgaaaac aaatgatgtt caggcaggga
     2941 atcaagctct tttagcatta gaagaaaaaa tccaagaacg agtaggggaa tatttttatg
     3001 gatacggcga tgataattcg ctagcaaaag tcgtagtgga actactgaaa gaaaacaagc
     3061 agactgtaac agcagcggaa agtttgacag ccggcgcctt ccaagccgca ttaggcgaca
     3121 ttgctggtgt atctgaagta tttccaggcg gattcgttac ctactcgctc caaacaaaag
     3181 ctggttttct ggagattgat ccggagctat tagcagaata tggaacagtc agcaaagaat
     3241 gtgtggagca gatggccatt caagcacgtg agatagcaag aacagattac agtgtagctt
     3301 tcaccggagt ggctggaccg gacacgttag aaggtcaatc tgcaggaaca gtatggatcg
     3361 ctatttcatc taaagacggc gtggtttcgg aaaaatatca ctttgccaga gatcgtgctg
     3421 ctatcagata taatgcggtt atgcaaggat ttgatctttt gagaaaacag cttttaagaa
     3481 aatgataaat gcgaatattt gttcggtttt ttcttgcttt ttttcactaa aaaagatatg
     3541 ataaggataa tgaaatgttg ctctgatttg taaatataag gaggattatc gtattggcag
     3601 atgatcgtaa agcagcctta gatgctgcac tgaaaaaaat tgaaaagagc tatggtaaag
     3661 gctctattat gaaattgggc gaaaaaatcg accaacaaat ctctacaatt ccaagtggct
     3721 ctcttgcgtt agatgtcgct ttaggtgtcg gcggttaccc ccgtggacgt atcatcgaag
     3781 tatatggacc tgaaagttca ggtaaaacaa cagttgcact acacgctatt gcagaagtac
     3841 aaaaaaatgg cggaacggcc gctttcattg atgctgagca tgcgttagat ccgcaatatg
     3901 cacaaaaatt aggtgtgaat atcgatgaac tacttctttc acagcctgac acaggagaac
     3961 aaggtctaga gatcgctgat gctttagtat caagtggggc tgtagatatc gtagtagtcg
     4021 attcagttgc tgctttagtt ccacgagcag aaatcgacgg cgaaatgggt gactcacatg
     4081 tcgggttaca agcacgtttg atgtctcaag cattgcgtaa actctctggt tcgatcaaca
     4141 aaacaaaaac aatcgctatt ttcatcaacc aaatccgtga aaaagtaggc gtgatgtttg
     4201 ggaatccaga aatcactcct ggaggacgcg cattgaaatt ctacgcaacg atccgtttgg
     4261 aagtacgtcg tgcagaacaa ttgaaacaag gtacagatat tgttggtaac cgtacaaaga
     4321 tcaaagtcgt aaaaaacaaa gtagcacctc ctttcaaaat cgctgaagta gatgtcatgt
     4381 atggtttagg gatctcacaa gaaggagaac ttctagacat ggcagtagaa aaagacattg
     4441 ttgataaaag cggtgcttgg tattcttata aagaagatcg gattggtcaa ggacgggaaa
     4501 acgctaaaat ctatatggct aatcatcctg aaatgatggc agaagtttct gctctagtta
     4561 gagcagctta tggtattggt gaagaagtcg ctgtcccaga agatgaaaaa ggacaagaag
     4621 aattgccttt agtagaagaa taaaaattat gataaacaaa aacggctggt ctgaagagta
     4681 ggtactctcc ttgacctgcc gtttttttat tgaataaaga aatcgtgagt tatttaatta
     4741 tacgagtggg tacataaaat aaaaatatct gttcatctac aaagaataat ttaatagaaa
     4801 agtttctcaa tttaaaggtt tacctatatg aaactagtgt catttatttt aaaaaccatc
     4861 gtttaatgaa taagtagaaa gccgtttctt tgtttattta aagttgacac tacggggtac
     4921 aaacattaga ataaagttgt gcatgttttt tgtacacata tatgcgaaga tgcatattga
     4981 cgagtttaga ataatttatc atagaagctg tatagatgat aaaaatgaaa aagatagtat
     5041 ggaggtggat tttatggtat tcaacattct cctcgctatc atcggtttaa ttgtcggtct
     5101 tggtttaggg tttatggtcg caaagtcacg tcacgagaaa gaaatagctg gtgcacaatc
     5161 atcagctgct ggaatcatcg atagtgcaaa aaaagaagca gaaactctaa aaaaagaagc
     5221 attgttagaa gctaaggaag aaaatcagaa ataccgttct gaagttgaaa gtgagctgag
     5281 agaaagtaag ttagaattaa aatctcaaga gaatcgtttg attcaacgcg aacaaacgct
     5341 gaatcgtaaa gatgactcgt tagaaaaacg cgagaactca cttgaaagta aagaagaagc
     5401 acttagttca aaacagcaat taattgaaga gcgagaaaaa gacgttgaaa aaatgattga
     5461 agatcaacaa aaagaactag aaaaaattgc tggtttatca aaagaggacg cacgagatgt
     5521 catcatgaaa tcgactgaag aagagctgaa tcatgagtta actctaatgg taaaagaatc
     5581 tgaacagcgt gccaaagaag aagcagatcg caaagcgaaa aacttattat cattagcgat
     5641 tcaacgctgc gcagcagatc aagtttcaga aacgactgtt tcagtcgtta cccttccgaa
     5701 tgatgaaatg aaaggtcgta tcattggacg agaaggtcgt aatattcgaa cgcttgagac
     5761 gctgactggt atcgatttaa tcatcgatga tacaccggaa gcagttgttc tttccggatt
     5821 cgatccaatc cgaagagaaa tcgcacggat gaccttggaa aaactgatac aagatggacg
     5881 aatccatcca gcgcgtatcg aagaaatggt cgagaaatct cgtaaagaaa tggatgaacg
     5941 tattcgcgaa tacggggaag aggcagcgtt tgaagtcggt gctcatacac ttcatccaga
     6001 tctaatcaag atcatcggac gtctgcgatt cagaactagt tatggacaaa acgtcttgaa
     6061 gcattcggtc gaggttgcaa aattagctgg tatcttagca gcagagcttg gcgaagatat
     6121 ccaattagca aaacgtgccg ggttgctcca tgatattggg aaagcacttg accatgagat
     6181 tgaaggatct cacgttgaga tcggtgcgga attagctgcc aagtacaaag aaaacccagt
     6241 agtaatcaac gcgatcgctt ctcatcatgg agatgtggaa gctacttctg ttatctctgt
     6301 ccttgtagct gcagcagatg cgctctcagc agcaagacct ggtgcgcgaa gtgaatcatt
     6361 agaaaactac atccgtcgtt taagaagtct ggaaaatatt tcgaacagct tcgccggtgt
     6421 agaatcaagt tacgcagttc aagctggacg cgaagttcgg gtaatggtca agccagaaga
     6481 aatctcggat ttagacgctg ttcgtttagt acgtgatatt cgtaaacgaa tcgaagaaga
     6541 actggattat cctggacaca tcaaagtaat cgttattcgg gaaactcgtg caactgatta
     6601 tgctaaataa taaaaaatac aaaagaagag ccgcggcaaa agccacagct cttctttttt
     6661 tgtaaggcac gaaaaaacat tgctatcatt attttttatt caacatctca agatctttct
     6721 cgatcgtcgt attgattcct ttgatgattg ctgggatgaa tcctgcttct tctaaggcaa
     6781 ctacacctgc aacagttgta ccgccaggag aagaaacttg gtctacaagt tcccatggat
     6841 ttttatcact tgaaaggaat ttttgacaag aacccataat tgcttttgca gcaatctcag
     6901 tggctgtctg tttggacaaa ccattcagta caccagtccg tcccatcgca tcgatcagca
     6961 tgtaaacgaa tgcgggtgag ctgccagcaa ggccaatgaa tgtactgaaa tctgattctg
     7021 ctaaaggata gacgctgccg attgcttcaa aaatcatcgt agcttgctga agctgttcct
     7081 ccgtggcagc ttcatttttg caaatcgcag aaacgcctgc tcctacgctg acattgacat
     7141 tcggcatcac acgtacgatg gaggcctgag gatcttcaag cgctgtttct aaatctatta
     7201 atgattttcc tgaagcaata gaaatgaata agactttctg gctatattct cttagttctt
     7261 ccaatacttt ttcaatgatt tttggtttca cggctaaaag aacgatatct gattgtgatg
     7321 caacttcctg attacttttg catgcatgga cgccagtttc agcagcaaat ttttgtgcat
     7381 tttcaaaatg tccactggaa acgaacacct cttctttcga gactgttcct ttttttaata
     7441 aaccatcaat aatagcttgt gccatattcc ctgtgccgat aaatcctact ttcatgttat
     7501 cctctccttt agcattagga tatctttctt tttctatcat cgcaagcaaa gaacaggtct
     7561 aaagtggaaa aattaaaata aataagataa cattggggta tgacacaaaa gtatacaagt
     7621 taggaatggg aaaatgtgaa aaataaacga tacttgatgc tgcaatggat aaaggaacat
     7681 agtctgattt taaaactgat ttttttcggc tccgtgttag tctttgtggc aaatcaagtc
     7741 gcaaatattg ctaatggcat gagttggcaa gatatttggc aaaggatggg acaacaaagc
     7801 cgcatgacga tcctgctcat gattctggct ggactgctgg gtgtcactcc gatgttgttg
     7861 tatgattggg tgacgatcag agtcttagaa aaacaaggaa aaccaaaaat ggaaagaaaa
     7921 gagtttcttc ttgctgcgtg ggtaacaaac acgatcaaca atctcgccgg ttttggtggt
     7981 gtggtaggag caagtctgcg ggctagtttt tatggaaagc agacaaatag aaaaatggtt
     8041 ttagccactg tatcaaaagt cgcactattt atgctgactg gtttgtctgt atggtcgttt
     8101 cttacattta ttgatgtctt ttttattcag tcagaaagta tttttcgaag ctattggatc
     8161 tggcttttcg gcggtagttt tttagcacca gtcattttct tttttgctta tctaaagaga
     8221 aagacattgt tcttcgagtt ttatccaaaa ggcttgcttg gtctgctact ggcatccttt
     8281 ggacaatgga cgggagccct tgcggtattt ttgatcatcg gcaagttgat gagaatagat
     8341 atttcgatgc tttcgatcta tcctatgttc atgattgcta cattgatagg aatgctgacg
     8401 atggtcccag gtggaatggg gacttttgac gttttgatga tcttaggaat gagtcaattg
     8461 tctgttcggc aagatgtagc agtagtttgg ctattatatt atcggttgtt ttattatgtt
     8521 ctacctttta tgacgggact tctattgttc atcacacata caggagtcaa aatcaatcga
     8581 tttttagata acattccgcg actgtttttc caaaaggttg cgcatatcgt gctcgtaagt
     8641 gctgtctatt ttgcggggat catgatggta cttctatcaa caatcactaa tctgtctgca
     8701 atcagtcggc tatttcaagt attattgccg ttctcattca attttttaga tcagacagtg
     8761 aatctattgg ttggcttttt attactcggc cttgctagag cactttcagt aaaagtcaaa
     8821 aaggcatttc tgcctacgat tgctttacta tcttttggta ttttaaatac aatcacacgt
     8881 acgttatctt ggcagttgat attggtatat gtcttgattc tgttagctgt gtggttatcc
     8941 agaaaagaat tttatcgaac aaaattcgtt tattcttggg gagctataat ctttgatgct
     9001 agcttgttcg gctttttgtt tatcgtttac gcaattgcag gctatcacag cggacgcttt
     9061 ttagtcggtc gaataacagg aaatcatttt attttgtttc cttcagatga tgtgtggttc
     9121 tcagggctta ttggtttggg catctcattg ctagctttag tggcgcttta tcaatattta
     9181 gctgaagata ctacgaagct tggcagtgag tacgaaaaag accgttttga ccgtttattg
     9241 gaaacatacg gaggcacagc tacaagccat cagcttcgct tgtcaggtta cagttatttt
     9301 tattaccaag aagacgacca agatcaagtc gtttttggtt atcagatcaa aggaaacaaa
     9361 tgctttgttt taggagatcc agttggcaat cctaaaatga tgcgggcagc aacgaatcag
     9421 tttatgaaac aagcagattg tttaggctat cagcttgcgt tttataagat atcagaaagc
     9481 tacgtagtga tgcttcatga attgggtttt cacttcacga aagtaggtga agcagggatt
     9541 gttgatctag caacagaaga cttgtctcgg ctgagtcagc aacttgaata tcaacgtttg
     9601 atgaaagaag gataccattt tacttggtac gatcaactgc cggaagaatt acatcaagct
     9661 gtaaatgaag tttcacaaaa atggcttcaa ggtgcacgag aaaaatactt ctctgtggga
     9721 cgatttgata aatcatatgt attatctagc ggtgtcggta ttgtccgtaa agatcaaaaa
     9781 gtcgttggct tcatcaccgc tcagccgatg acgaagaagc aggcgggata tgatttgttg
     9841 cgggtcttac ctaaagaatc tgaggaatta gctgactatc tgttagccaa tctgtttatt
     9901 gtatacgaga aaatgggcta ctcggaagcc aatctaggct tagctccatt agctaatgtt
     9961 ggagaaacag atttttcttt ctttcaagaa aaagtcatgc atatcgtata taattatggt
    10021 aattttttct attccttcca gtctgcttat gaaaagaaat taaaatatgt cgatcattgg
    10081 gaaggacgtt attttgctta taggaaagga agtaatttcg gatttgctac catgcagcta
    10141 ctcagtctga taggaaaaga aaaaggaaat gttccgagtt tggcagaaga agtgctcaca
    10201 gaattataag aatcactaga acttgtttgg attatgaaga gaaaattctg tgaaagctgt
    10261 tgaaacgatg acaaaatcaa ccactaggca gacataagct atacttttat ttttccctat
    10321 gatagaatac ttgcgactta gacttttcga atgggtgaga ggatggaatt gattgaaaaa
    10381 gcgccagcca agataaattt aggactggat gtattatata gacggcaaga tgggtatcat
    10441 gagttggaaa tggtgatgtc gagcgtagac ttggcagatc atctattttt tgaagaaata
    10501 acagaagaca aaatcattgt agaaacaaat aaagcatttt taccggtaga tgaacggaac
    10561 aatgtttatc aagctgcttt attgctaaaa aataaataca atatcaaaac cggtgtcaag
    10621 atctttgtta agaagaatat tccagtagca gctggattag gcggaggaag tacagactgt
    10681 gcagctgctc taagagggat caaccaatta tggaatctgg gctgttcgtt agaagaactg
    10741 gcagaaatcg gtttggaagt cggtacagat gttccttact gtgtctacgg aagtacggca
    10801 tttgtcggtg gcaaagggga agaaatccaa gtattacctt ctatgccaca gtgctgggtt
    10861 gtgttggtca aaccaagaat gagtgtctct acacgtacag tgtttagaga agtagatacg
    10921 caagccatcc aacatcaaga catccacgga cttgctgaag ccatccgaga gcaagattat
    10981 cagcagatga tcaaaaaaat gggaaacagt ttagaagata taacgggaaa gcggcatcca
    11041 gtcatccatc aaatcaaaga gcgtatgatc aagtacggag cagatgctgc attgatgagt
    11101 ggaagcggac caaccgtttt tgccttgtgt caacattatt caagagcgca gagagtatat
    11161 aatgggttaa aaggtttttg tgaggaagtt tatttagtac gaaccctgca ataaaatata
    11221 agaagtaaaa taggtgatga aaaactgagg aatcctaaaa tggtattctt cagtttttta
    11281 ttgtagtgaa atggctagat atgagcagca actcatccat tttacgattt aatcaagaat
    11341 atacatgctt aaatcagaaa ttgatggatt tctattgact gtgttttttt attttgatac
    11401 attagttcac gttaataaat cgtaaaattc cgatttgttc attgaaagga gcatataaat
    11461 gaaaaaatat cttttgacag ctacggtaat gataggtgct ttactgtttg cagcatgtgg
    11521 aaatacgaac aaggaagcag acaaaaaaga ggatttgacg atcgttacga ctttttatcc
    11581 gatgtatgat tttacaaaag agatcgtagg ggatgaagga aacgtgaaac ttttgatccc
    11641 tgctggaacg gagccgcatg atttcgaacc aagtgcaaaa gaaagagcag aaatttccga
    11701 tgcagatgtt ttcgtgtata atagttcaga catggaattt tttgtggatt cgttaaagga
    11761 ttcagtggat agcaaacaga ccttgatgat cgaagcagct aaaggaatcg accgtttaga
    11821 atcgcaagaa gcagacgaac atgaagaatc agaagaaggg catggtcatt cacatgaata
    11881 cgatccacat gtttggctcg atccggtatt agctataaaa gaagtcagaa cgattgctga
    11941 agaattaggc gaaaaatatc cagataaaaa ggaaactttt acgaagaatg ccgatgctta
    12001 tatcaaaaag cttgaagcac tggatcaaaa atactccgat gagctaaaag atgcaacaaa
    12061 ccgcacattt gtcactcagc atgctgcttt tgcttattta gcgaatcaat acgacttaaa
    12121 acaagtagcg atttcgggcg tttcaccaga tcaggaaccc acaccaagtc gcttagctga
    12181 gctaaaagaa tttgtgaaga aaaacaacat caaagtgatt tattttgaag aaaatgcctc
    12241 ttcgaaggta gctgagacat tgtcgaatga aacaggagtg aagctagaag tgttgaatcc
    12301 tttagaaagc ttgactaatg aacaaatcaa agcaggcgaa aattatattt ctgtcatgga
    12361 aaaaaatctg aaagctttaa aagaaagtat caattaagtt ttagaatgga ggttgtaagg
    12421 tgcgttatat cgaaatatct gatttgacct tttactatga tgatgaacct gtactagaag
    12481 atgtctctta tcatgtagat gcgggtgaat ttgtcatttt aactggagaa aatggagcag
    12541 ccaagtcaac cttggtgaaa gcatcgttag gattactgaa accttcaaaa ggaatgataa
    12601 agattgctga gaaaaatcaa gaaggaaaaa aattaagtat cggttatatt cctcaacaag
    12661 ttgcatcttt caatgcaggt tttcctagta cggtgatcga attagtccgt tcaggaagat
    12721 atccaagagg ccgctggttc aagcgtctct ctgaaaaaga tcatcaacat gtacagcgtg
    12781 ctttagaagc tgtagggatg tgggatatgc gaaatcggcg aataggtgaa ttgtctggcg
    12841 gacaaaagca gcgaatcagt ttagcgcgta tttttgctac tgaccctgac ttatttgttc
    12901 ttgatgaacc aacaacaggt atggatgaag aatccagaaa tgaattttat cgtcttctcc
    12961 gacatagtgc gcatgtccat gggaaatcag ttctaatgat cacccatgat catgaagata
    13021 tcaaacagta tgcggacaga cagatccgac tggtgagaaa ggaggattct caatggcgtt
    13081 gctttcatat gaattcatga gaagagcgtt tcttgccgct atcttcattg ccggaatcgc
    13141 cccgatgcta ggtgtgtttt tagtgatcag aagacaatcg ctgatggctg atacgctttc
    13201 tcatgtctct ttagcagggg tagcgttagg attctttttg aaccttaatc ctacacttac
    13261 gaccatgcta gtcgttgtat tagcagctgt gatattggag tatctgcgaa cgatctaccg
    13321 aacctattca gaaatctcca ttgccatttt aatggctggt ggtttggcgc tagcgttagt
    13381 attgatgaat ttgagcggag ggaattcagc aacaagtatc caatcctatc tgtttggttc
    13441 gatcgtgacc attacatggg agcaagtcat ctttttagga atcttattcg ttgccatcgg
    13501 tatattgttc tttttattca aacggccgat gtatgttctg acttttgatg aagataccgc
    13561 ccacgtggat gggctacccg ttcatttgat gtcgatgatg ttcaatgtga ttactggtgt
    13621 ggctattgct gtgatgatcc cgattgccgg tgcacttttg atatccgcaa tcatggttct
    13681 tcccgcagca atcggtatgc gattaggcaa aagcttcaat gtcgtgattg ctatcagtgt
    13741 ttgtgtagga tttctaggga tgctgtcagg actaactagc tcgttttatc tagatacacc
    13801 tccaggagca acaatcacat tagtctttat tgctttgttc ctgattgtaa atatcatcaa
    13861 acgaatattg gttgccttca gaagaaaaaa taactaaatt ataaagaaaa gaagccttat
    13921 aaaaacgaaa aattttttat aggcttcttt ttattttaat aaataccgaa ctattttctt
    13981 attttttcat aaaatgctcg aatttttgaa gaaaaactta tataatgtaa aacgataaaa
    14041 tttcttaaaa ggagttggaa taggtgaaag tacgtcgcag tgaacgtcta gttgacatga
    14101 cacagtattt attgaatcac ccgcatgaat tgatttcctt aacaagcttt gccgaacgtt
    14161 atgaatctgc taaatcttct atcagtgaag atttgggaat catcaagaaa acatttaaag
    14221 aacgagggta tggaaccttg gaaacaatcc cgggtgctgc aggtggtgta ttgttcatcc
    14281 ctgaaatttc atatgaagag gcgaaaaaat atattgaatc tctagcagaa cgtctatcag
    14341 aacaagaccg tctgcttcca ggaggatatg tttatctctc tgatttgtta ggagaacctg
    14401 aactgctgcg tcaagttgga agaatcattg cttctaaata ccttggtgaa aaaatcgatg
    14461 ctgtaatgac tgtagccaca aaaggcgtac ctattgccca agctgtgtcc tattatctga
    14521 atgcgccatt cgttattgta cgccgggact caaaaataac agaaggatca acagtaagtg
    14581 tcaactatgt gtctggatct tctgaaagaa ttgaaaagat ggaactgtct aagagaagtt
    14641 tgaaacgtgg ttcacgtgtc cttgtagtgg atgatttcat gaaaggcggc ggaacagtca
    14701 acggaatgaa gagcttgatt gaagaattcg aatcagaact tgttggcatc actgtatttg
    14761 ccgaatcaaa attcaacggt catcgcgcca tcgatgatta tacgtcactg ctttatgtca
    14821 aagatgtaga tactcaaacg aaaaatatta cggttgtgcc aggaaattat ttttcagaat
    14881 aagaggttgt gaaaaagccg cttagtttcg agtagtaaga aaaattttgt gaaaatggct
    14941 ttccacattt tttcataaat ttggcttaat accgagaaac tggcttttga acaccgttta
    15001 tctagaggtt gtgaaatcag cgtcctgtcc tgagtattag ctcaataaac ggaaaaattg
    15061 ctcctcatat tttgccgttt attgagctaa taccgaaggt cagctgattg aaaaacgttt
    15121 aatgaagagg ttgtaagcct accgtttaac tctaagtcac aagaaacgat ttcaaaaaac
    15181 agcttttcat gttttatgag tagtgtgact tgtgaccgag gagttggcag gagagcaccg
    15241 gttaccaaat agataggccc gttatggtct atctattttt ttatccagaa aacgctggga
    15301 taaaagaaaa tcacttgttt tgttgaaaaa atgggtatac tgggataaac tgaaatcagc
    15361 aaactatatt taggagtgac gcagttggac gcacgttatg caattatttt ggcggcaggg
    15421 aaaggtaccc gcatgaagtc aaaattatat aaagttttac atcccgtttc tggtaaacct
    15481 atggtagaac atatcattaa tcgggtcagt gaaacgaaac cagatgaagt gattacgatc
    15541 gttggacacg gagcagaaca agttaaagca caactaggag aacgcagtaa atatgcgtta
    15601 caagcagaac aattgggaac aggacatgct gttttgcaag ctgcttcttt cttggaagga
    15661 aaaaaaggaa caacgttagt tatcagtggc gatacaccat tattgactac tgaaacgctg
    15721 aacaatcttt ttgaatatca ccaaggaaaa aatgcaagtg caacgatctt gacggcacaa
    15781 gcagaaaacc caacaggata tggacgaatc atccgtgacc atattgggat cgtagaaaaa
    15841 atcgtggaac aaaaagatgc aacaccagaa gaagctcttg tacaagaaat caatacagga
    15901 acttactgtt ttgacaacga agctttgttc gatgcgttaa gcaaagttgg aacaaacaat
    15961 gctcaaggtg aatactatct aacagatatc atcgaaatct taaaagaaga aggccataca
    16021 gttgctgctt atcaaacgga tgattttgaa gaatcaatgg gagtcaatga tcggatcgcg
    16081 ttagctaaag caaatgagat catgagaaag cgaattaacc agatgcatat ggtcaatggc
    16141 gtaagctttg tcgattcagc aacgacatac atcgatgcag gcgtagaaat cggtccagac
    16201 acattgatcg aagcaggtgt acaaatccaa ggaaatacag tcattggttc tgattgtgtc
    16261 atcgggtcac attcaaaaat cgtagacagt aggattgagg atcacgtcgt cattgaaaac
    16321 tcagtcattg aaagcagcca tgtcaagaaa catgcagatg tgggacctta tgcacattta
    16381 cgaccaaaag ctgaaattgg cgaaaacgtc catatcggaa actttgtgga agtgaaaaat
    16441 gctcaaatcg gcaaaggaac taaagtcggt cacttgactt atgttggtga tgctacgctg
    16501 ggagaagaaa tcaatgtcgg atgtggtgtt gtcttcgtca actatgatgg aaagaacaaa
    16561 catcatacga ctgtcggcga ccacagcttt attggttcca gcacgaatat catcggacct
    16621 gtcgaggtag ctaaaaacag ttcgattgct gccgggtcaa caattacaga caatattcca
    16681 gaatatgcgc tggcaattgc gagagcaaga caagtcaata aagaaggata tgcaaaaaaa
    16741 ctcccttatc taaactaaga cttttgcaaa atcacttgat ttatttaaaa aatatgacta
    16801 ctatttatta ggtagaattc tagagttgtg aaaaagaaag tggaggtccc catgtcaaaa
    16861 cattactttg acccaagatt aaaaattttt gcgttaaatt ccaatcgccc attagcagag
    16921 aaaattgcag cagcagtcgg cgtcgagtta ggaaaatcat ctgttactca atttagtgat
    16981 ggagaaattc aagtaaacat tgaagaaagt attcgtggat cgcatgtcta tgttattcaa
    17041 tcaacgagca gcccagtcaa tgataacttg atggagttgc tgatcatgat tgacgcactg
    17101 aaacgagcaa gtgcaaaaac aatcaacgta gtcatgcctt attatggcta tgcgcgtcaa
    17161 gaccgtaaag cacgtgcacg ggaaccaatc acagcgaaat tagtagcaaa catgattcaa
    17221 aaagctggtg caacaagaat gctgactttg gaccttcatg cagtccaaat ccaaggtttc
    17281 tttgatattc cagtcgatca tttgatgggt gcgccactta ttgccaatta tttcttagaa
    17341 aaaggaatca aaggcgatga tgttgtcgtt gtttcaccag accatggtgg tgtcacacgg
    17401 gcacgtaaat tagcggaatt tttgaaatct ccgattgcga tcatagacaa acgccgtcca
    17461 aaagcaaatg ttgcagaagt aatgaatatc attggacaag tcaaaggcaa aacttgtgta
    17521 ctgatcgatg atatgatcga tacagcaggt acgatcacat tggctgccaa tgctttgaaa
    17581 gaagctggtg caactagtgt gtatgcttca tgtacacatc cagtattatc cggccctgct
    17641 ttacaaagaa tagaagattc agctatcgag cgtctagttg ttacagactc gatctatctt
    17701 ccagaagatc gtaaaatcga caaaatcgat gaagtaagtg ttggcggact aatgggagat
    17761 gcaatcaaac gtatccatga aaacaaacca gttagtccat tgtttgaaac aaaaaataaa
    17821 taataaataa actcacaaag gggagtccaa ttgggctgag attgaatcta tttctaaacc
    17881 cttcgtacct gtatcggtta tgcgagcgta ggaattgtga ataaacagct gtttgactat
    17941 tttcgaaagc tgcttcctcc tttgtgactt ttttattcaa aggaggaagt ttttttatgg
    18001 gaaaaaatag agtttgggta gaaggaacga ttgttgcagc gttagcgatg gtgttgtcgt
    18061 tgattccgat ccagatcggc agcagttttt ctatttcatt agggcagatt ccattgactc
    18121 tttttgcgtt aagaagaggg acaaaggcag ggatgctggc aggtttcatt tggggcatcc
    18181 tacattttcc gttgggacaa gtttattatt taagtgtcgt tcaagtgttg attgagtacc
    18241 cgattgccta tacttttgca ggcgcagcag ggctcatggc ttcaaatgtc caaaaaggct
    18301 atgtagacga taatacgaaa caaattagaa aaagcatcgt gtttggtgca ttgattggcg
    18361 cactttccag atatttttgg cattttattg caggagtggt tttctgggga gcgtatgcac
    18421 tatgggggat gaatccttgg ctcttttctt tcgtaatgaa tggtgccagc gggttgagta
    18481 cagctattgt aacgatcatc gttttattag ggatccaaca aacgactcct tcgatttttg
    18541 tgccagataa acgacaggag ctgtaaataa aaaagaagca agaaacttga gacagaatca
    18601 tgtcctagtt tcttgtttcc tttttatttt ttgataagaa atagatctac ttcaatggca
    18661 gataagaaag atcggcatct tctaccaatt gccagtgtcg atcggtgaca gagtagatta
    18721 aagtagcgat gatttcctct tctcgatacc gggtgccttt taatttccaa atataatctt
    18781 caatcacata gattgggata cgatatttac catcgatcaa accaacttga tcaaaagaaa
    18841 aaatgatccc ttcctgcatc aaattacgca tttctttcaa taattccaca ctgatttttt
    18901 caacaggtgt ttcttttttg aaaaaagatt tttttacatt gatcttatct gacaagatcg
    18961 aacttaattt actagcaaaa atcctataaa attcatctaa atagcgataa taaatccgat
    19021 tcagctgatc attaagatcg aagtatacaa aattattctg tagtttataa aaaaacggcg
    19081 aatgaagatg tgttttcata tgaccaaaat ataataattc tgaaatttct aaaggtgtca
    19141 attctttcag catcgttaaa tcagtaaagt cgatccactt caattcatcg aatccacgcc
    19201 tgttacgaga agaggaaaag aaacgctgaa tattttctgt accacggatt actttcagcc
    19261 ctgtatggga ttcatattct ccagcagaag cagatggatc aagcaagagc aagttttgag
    19321 gaaaatgcac gatactgttt gcaaagtcag tagcagtcag cccttttgtt aaaacagcat
    19381 tacttgtggt atcaatatgt acataaactg actcagtcat catgttcctc ctcttatttc
    19441 ctcttaatct cttttatttt acaacatttg cattaaaaaa gcttgtttct tttattttaa
    19501 aattccatta caaaatcacg ttttcattga aaacactctc attttcaatt aatttttgtg
    19561 atttttgtat ttttttattt aaaggaggtt ttatgaattg ttttcttatg gaatctacaa
    19621 agtcgaatac gagactcatg acctttttat gacgtactgg tatacttgaa gaaaaaggag
    19681 agcgcgatgg agataaaaaa agtttacttt tcacagaaaa tgtcgctgta ttcgcctatc
    19741 cagcaagcaa aacaacccct tccatttttt caaagtcatc aagaaagtag agacgctttt
    19801 tggcgttctg caaaaaagca atgtgctttt aaagcagtat cgaaaaaata taccgtctat
    19861 tttactattg ctaaaagtag aggagatgtt atttttaata atctgttgat tgaaggtgaa
    19921 acttttgaat ggaaaaaatt ttttcggtat atggaagctt gcgctcgttt ttttattaaa
    19981 gaaaactgtt gttttttatt tcaatctcct ctatcagaag aatggttgcg tatttttcat
    20041 aaaaacggct atcaaggaac cacacaatta aataaaaagc tggtctatca tacagcactt
    20101 gttttaggtg gaggaggagc gcacggtgct tatcagattg gtgtgtggca ggcgctgaag
    20161 gaacatgata tcaactttga gattatcaca ggtacctcgg taggtgcgtt gaacggcgca
    20221 ttgattttac aaggagatat gaaaaaagct gtgaatttat ggaaaaagct gtcgacaaga
    20281 caagtgcttg cgttaccaga aatggctgca gttgaagacc ttcgtgaacg cttctatcga
    20341 gaaagacgcc aaatgacgaa aacagcacta attgaaggcg gggtttcttc tgctccattg
    20401 gaaaagatgg tcaaagacaa tttggatttg tccttactca aacataatcc caaaatacgt
    20461 ttatatacgg tgtctactaa actgccagaa ctagcggaag tagtgactga tatccaacag
    20521 acaaaaacag aagaaatacc agattggata ctcgcctctg cttcatttta tcccgctatg
    20581 gcttatcgaa agatcggaaa aaataaatat gcagacggtg ggtatcgcaa caaaatacct
    20641 attgatattg cgattaacaa aggagcaaca gaggcatttg tcgtagatgt gcaagggcca
    20701 ggcccagcga aaaaaattcg tatgccagac atttttatcc attggaaatg tcaaacattg
    20761 tggacactag gcagctttct gctttttgat agccaaagga accaattaaa tttacaatta
    20821 ggctatctgg aaatgaaaaa acgattaggc tattactatg ggaactggta tacatttcat
    20881 tctgttaaac ctgcaagaac atactggcga ggatttttgt cttacttaac aaaagaaatt
    20941 cagttagaat tatctttttt caaaagcata aaattttggc aaaaactgcg taatttatat
    21001 aagaacaggg tcgtaccaga gacatgcgga ttggcgatgc tagaactttt agcaaaaaaa
    21061 cattttttac tgcccaatga aatctatcaa gtagacatga tgattcaaat gatctgccaa
    21121 caagatataa aaagtatgcc ggatgattta ttggcacaaa ttggtcaatt aagtactgaa
    21181 gaatggcgaa ggtatcgaaa ataccagcaa aaaatacaaa atgagcgcac gaaaactgtt
    21241 tattttgatt atctgctaca aggaaaacgc aaggatcgtt tgcagcatga attgcttaag
    21301 caaccagttg acacattact gatcctatat ttatattatc tcaaggagga acaaccatgg
    21361 cacaagaatt ttcatatgaa atcgtagaag aaatcgctat attgtcagaa aacaacaagg
    21421 ggtggcgtaa agaactgaac ctagtcagct ggaatggtcg cccgccaaaa ttcgatcttc
    21481 gagattgggc gccagaccat gaaaaaatgg ggaaaggcct gacattgacg aacgaagagt
    21541 ttgaacaact gcaaaaagca atagaaaata tgtaatgagg aaggaccctg tcgaatgaaa
    21601 agtatgactg gttttggcaa agccatccgc gaaacaaaag cgtatcagtt agatattgaa
    21661 ataaaaagtg tcaaccagcg ttttctagat gttcaaatac gaagtcctaa aatcctgaac
    21721 tttgttgaaa acgattttcg tcagcttatt aaacagcagt taagccgcgg tcgaatcgaa
    21781 gtatttgtga atctagctta tattggcaaa aacgaaaaac agatcgtcat caactgggaa
    21841 ttgattgatt cacttatcac tcaattgaca gacggaattc aacaacgata cggtagtcaa
    21901 aatcaagtgt caattagtcg gatggcggaa cgcttcgtgc tggacgaaaa cttcgtaacg
    21961 gtagaagaaa aaccggaaga gagcatggaa gaattggcac agttagctct agatacattg
    22021 aaaagcgcac ttgattctat tgaggcgagt cgagaaaaag aaggtcaagc tttagcttta
    22081 gtgttgaaaa agaatactga agaattcaaa gaagtactaa aaaaactcga tagttttgta
    22141 gaaatctatg aaaaggaatt tcaaataagg tatcaaaaaa agctggagga atttcttgga
    22201 gcgaaagtcg atgagcaacg gttgttgacg gagctagcca tcttgttgga acgcggagat
    22261 attcacgagg aactggatcg tctggcgatc catgtacaaa aactagatga acttatcgaa
    22321 gcacaatgtc ctgtaggtcg ggaattagat ttcctgatcc aagaaatgaa tcgggaaatc
    22381 aacacgatcg gttccaaatc aaatgcaatc gaaattaaaa atcaagtcgt ccagtctaag
    22441 acgatcttag aaaaaatcag agaacaagta caaaatattg aatgacgaaa gaggtagaga
    22501 taatgaaata ttatgacgta acgttccacg aattaagtgg aaaaactgtg gttaaacggg
    22561 atatcccttc tgaaaaaaac ggctttgacg tatggaaaga tgcctgtgca gactataatg
    22621 agaacgaact gtttattttg atcaacgacg gtgcttacgt gacgatgaat cggaaattta
    22681 ttgttcgaat tgacacagaa gaggtagaag atccgacaga aaaagcacgt tctcgaaaag
    22741 atgagatcat gggtgtagtg aatactttgt ccaatatggg cttttagcaa ggatgctgaa
    22801 caaagaaata caataaataa acagaattgg atttcttcgc ttagcttctt tgctctagga
    22861 atacatttaa aggttgtgac aaaattcgtg tcacaatctt tttttatttt gaataagtga
    22921 tgttcaaaaa ccattttctc ggtattaagc caaatttatg caaaaatgtg agaaactatt
    22981 ttcacaaaat ttttcttcat actcgaaact aatcggcttc ttcacgacct cttatacacg
    23041 gcgttcaaaa gcttgcatct gcgataataa ccccaaccaa acgaaaaaca tgagaagctg
    23101 tttatgtttg gctgttctta ttacttgatg caggacagct tttttacgac ctcttggtac
    23161 agggtagctt ttttaccaat ttctagctat aattaaagat aaccttgaaa gttgcagaaa
    23221 aaaagtgcaa aataaacgat aagtacaaaa agactagttg aaaggacgtc gtcatgtcag
    23281 agcgtggatt attaatcgtg ttgtctggtc catccggtgt aggaaaaggc acagtacgaa
    23341 aagcaatttt tgagagtgag gataatgatt ttcagtattc gatctcaatg actacccgta
    23401 aaatgcgtga aggggaggtc gaaggcgttg attatttctt ccgaagcaaa gaagagtttg
    23461 aatcaatgat cgaagctgga gagatgctgg aatacgcaga atatgtcggg aattattatg
    23521 gaactccttt gtcatatgtc aacaagacat tggatgaagg aaaagatgtt ttcttggaga
    23581 ttgaagtcca aggagcaaaa caagtcaaag aaaaagtgcc cgatggtgta ttcatctttt
    23641 tgactccgcc agatttagcg gaattaaaat cacggatcgt aggccgagga acagatgcag
    23701 atgaagtaat cgaaaaacga atgaaagttg caaaagaaga aatcgaaatg atggctcttt
    23761 acgactatgc tgttgtaaat gatgaagtgc cgctagcagt tgaacgaatc aaaaacatta
    23821 ttgccagtga acattttcga gtagatcgcg taatcggtaa atatattaaa atgttaaagg
    23881 agatgtagtc ttatgatgtt aaaaccttca attgactcac tattagatcg tgtaaattca
    23941 aaatattctc tagtgatttt agcaagtaaa cgcgcgcatg aattggatgc aagtgcacag
    24001 cctactctag attcttttga ttcagtaaaa agcgtcggtc aagctttaga agaaatcgat
    24061 gcaggaaatg tagtcaatga tccacatcct gaattaaaac gtgaacgttt gaagatggaa
    24121 gaagaggaac gtcaagcaca aaaagagcgt gaacaacacg aattggaatc acgtatccgt
    24181 gaagagcaaa aaatgtaata aagaaaaata gagcgaacat ctaaaaagaa aagcaaaccc
    24241 agctgtttcc ttttcaaaca ttcgccactt attgtctggt agaaaaggct gtgtctgaac
    24301 tttttgacac agttcttttt tttcttatct gctgttaatt ttttgttagg atttggcaga
    24361 agataagaca aatccatcga tctctagtaa aataggcaag gaactttttg tcgcttgata
    24421 agaaaagaca aaaaaaggag gaaacgaacc atgacattaa cagctgaagt cattgtggac
    24481 gtacctacta tgcagaccga tcagccattc acttatttgg ttccctctga aatggaaaca
    24541 gctttgcaag tcgggatgcg agtagaagtc ccttttggta atggcaatcg tcatgtccaa
    24601 ggatttgtta tggctatcca aaaatcagaa gaatcagcta atccatcttt gaaggcagtt
    24661 attcgtttgc tagaccttgc tccggtggtc aatgaagaat tactttcatt agctgattat
    24721 atgaaaaaaa ttacttacgc attcaaaatc acttgtctac aaacgatgct tccgagtgtg
    24781 atgaaagcag aatatgataa attgatctat cctttggcag acacaccaga agttcaggta
    24841 ttgtttgatc agcgagaagt gatttcctgg aaggaagcag aagaagaagg cagcctttct
    24901 caattgattc gctggcgcca agagcaatta gtagatatca aatatgaagt ccatacacga
    24961 aataaagtaa aaacgattcg tttggtccgt tctttgttaa ctgaaaagca aatcgaagaa
    25021 gaatgggcaa aattacggca aaatgccaag aaacaaaaag aacttctttt gtgtttatct
    25081 gaactgagcc aagaagaacc aattgcttat ttcaaaaata aaggaatcag tactgctgtc
    25141 ttgaatcaag gaaaagaaaa gggctggctg gagtttatag aatcagaacg ttatcgcgac
    25201 ccttataaag accgagtatt tgaccaaacg acggcattgg aactgaatgc agaacaaaaa
    25261 aatgctgtcg aacaaatcat cacagcaggt cagcagcaaa aagatcaggt ctttttgctg
    25321 gagggaatca ctggcagcgg gaaaacagaa gtatatcttc aggcaatcgc tgatgttttg
    25381 tcagaaaata aaacggctat tatgttagtg cctgaaatcg cattgactcc gcaaatggtc
    25441 gagcgattca aaggacgctt cggagaatca gttgcggtct tacatagcgg actttcccaa
    25501 ggtgaaaaat atgatgaatg gcgcaagatt gagcgggaag aagcgcaagt agtagtaggc
    25561 gcacgatctg ctatttttgc cccgctgaaa aatatcgggc tgatcattat cgatgaagaa
    25621 catgaatcaa gttacaaaca agatgagaca ccgcgttatc atgccagaga tctagcaatt
    25681 tggcgcagta aatatcatca ttgcccaata gttctaggaa gcgcgacgcc ctctttagaa
    25741 tcaagagcac gagctcaaaa aggcgtttat cagttattgc agttaaacca ccgagccaaa
    25801 gcagctgcac agcttccagc tattgagatc gtagatatgc gtgaagagtt tcagaatcat
    25861 cggacctcta ctttttctgc taatttgcag gaaaaaatac aaaatcgatt agacaaaaaa
    25921 gaacaaactg tcctactgct caatcgtcga ggatattcgt cttttgtgat gtgtagggac
    25981 tgtggatttg ttcttccatg tcctaattgc gatatctctc ttacactgca tatggataca
    26041 cgttcaatgc gctgccatta ttgcggacat gaagaaccta ttcccaatcg ctgtcctaat
    26101 tgtggcggca ataaaatccg ctactatgga acaggtactc aaaaagtaga agaggaactc
    26161 cgagaacttt atccgcaagc taggattttg cgtatggatg tagataccac tcgaagaaaa
    26221 ggggcacacg aacagatttt acaaaaattc ggtgcaaaag aagcagatat attattaggg
    26281 acacaaatga ttgctaaagg tttggacttt ccagaagtga cattagttgg ggtgttgaat
    26341 gctgatacct ccctgaattt accggatttt cgttctagtg agcatacatt ccaattgctt
    26401 acccaagttt caggacgagc agggcgagcg gaaaaagcag gcgaagtcgt gatccaaaca
    26461 ttcaatcctc aacattacgc catagaattg gctaaaaaac aaaattatga gcagttttat
    26521 cagcaggaaa tgcatgtacg gcatcgtgga ggctatcctc cttattactt tactgtgaaa
    26581 ataaccgcca gccatcctga agaacaagta gcggcaaaga aaatctttca gattgccaat
    26641 cagctaaaag aagtgttaag ccctcaaagc ttgctattag ggccaacgcc cagcatgatt
    26701 ttgagagtga aaaaccgata ttattatcaa ctaatcatca aatataaaca tgaaccgaat
    26761 cttcctcatg ttttggatga aattttgaat ggaagccaga aagaacagcg acaaggtctt
    26821 tttgtggcaa ttgacaacga accattaaac tttatttaag aaagggccaa attatgcgct
    26881 atcctatatt gatccaccct aatgataaat taaaaagaac tgctcagcca atcgatgtaa
    26941 tcacggacga gacaatcgct ttattagaca atttatatga gacaatgatt gcgaatgacg
    27001 gaatagggat cgctgctcct caagtaggac aaaacaagag aattgctgtg atcgaagtag
    27061 atgaaggcga gaaatttgaa ctgattaacc cagaaatcat cgaagcaaaa ggtgaaagcc
    27121 ttgacgtaga aggttgtctc agtattccgc atgtctatgg aacggtcaag cgagcagatg
    27181 aagtgaccgt gcgttattat gatcgagacg gagaagagat agaagtgacg gcttttggct
    27241 atttagcccg tgccttccag catgaaatcg atcacttaga cggtatcttg ttcattgaga
    27301 aaatgataca gcagatcccg gaagaagaac ttgaagaata tatggaggaa catcttgatg
    27361 actaagctag tatttatggg cacacctgct ttttctgtgc cgattcttga agggctgata
    27421 gaatcaggtt acgagatcca ggcagtggta acacagcccg accgtccagt tgggcggaaa
    27481 cgagtaatta cgccgacacc ggtaaaaaaa gcggctctga agcacggtat ccgtgtgctg
    27541 cagccagaga aaatctccgg ttcgccagaa atggaagaaa tcattgaatt ggtgcctgat
    27601 gtgatcgtca ctgcggcatt tggccaattc ttgcctgaaa agttattgca agtaccaaaa
    27661 ttaggggcaa tcaatgtcca tgcttcttta cttcctaaat atcggggagg tgcgcctgtt
    27721 cattatgcca tcatgaatgg ggagaaagaa actggtgtaa cgatcatgga aatgatcaaa
    27781 aaaatggatg caggcggaat ctatgcgcaa gaaagcatac caatcactaa acaagatgat
    27841 gtagggacga tgtttgaaaa gcttagcctt ttaggaagaa agctattgct agaaacattg
    27901 cccaatattt tggatggaca aaaaccagta cctcaagacg agtcagaagc aactttttcg
    27961 ccaaacatca cacgggaaca agaagcaatc gattggcata aaactgcaga agaaattgac
    28021 aaccaagtac gcggaatgcg tccttggcca attgcattca cgacctatga gggcatacgc
    28081 tggaaattat tatcggttga agtattggca gaaactacag acaaagcacc tggtacgatc
    28141 atccggaaag acaaaaaaaa tctatggatt gcttgcggta aacagaccgt cttagccatc
    28201 aatgaattac agccagctgg taaaggaaaa caggccgtac atgatttcct taacggaagc
    28261 ggtcagcaag ttcagattgg tcagcaggtg aaataatgaa aaaaatccct aaaaaaatga
    28321 aaaaatcagt acgatatatg gcattactga cattagagcg agtgaacaag ggtggcgcct
    28381 actcgaatct tttgttgaat gagatgatca aacaaagcga gctaaatcaa aaagatgtgg
    28441 gtttatttac agaattgatc tatggtacga ttagccatca gcgactattg gaattttacg
    28501 ttgcaccatt gatcgctaaa gcaaaaaaag tggatgactg ggtcaagatg cttctttatc
    28561 tttctgtata ccaattagaa tttttagata aagtccctgc acatgctatt ttaaacgagg
    28621 cggtcgaaat cgcaaaagta aaaggaaatc ctgggacagg gaaatttgta aatggtgtct
    28681 tgaggaatta tcagcgtcaa ggagcaccag atctgcaagg cattgctgat cctatcgaaa
    28741 gactttctgt caaaatcagc ctgcctctat ggatgacgaa acgactggtg gatcaaatcg
    28801 gatacgaaga aactgaaaag ctcggtttat cgctatatca gcctagccat gctagtgccc
    28861 gagtagatac acgccgactt tccagagatc aggccatcac tgttttgcaa gaagaagaga
    28921 tcacagctga aaaaagccaa gtcagtccat acggtatagt agcgaaaaaa gggcatttag
    28981 cttctagctc attgttccac gatggcgtca tgacgatcca agatgaaagt tcgatgcttg
    29041 tagcgccgtc tatgcaaatc gagcctgaac atcatgtctt agatgcctgt gcagcacccg
    29101 gaggtaagac gacgcatatt gcttcttttt tagatccgtc atgcggtgga gaagtggtcg
    29161 ctttagatgt acacgaacac aaagtcaaac tcattgaaga taatgcagca cgtctaggtg
    29221 tctcttctgc tgtctacgct caaaagatgg atgctagaga agtcagtaaa cagttcgaag
    29281 cagagcagtt tgatcggatt ttagtagacg cgccttgttc aggtcttggg ctgcttcggc
    29341 gaaaaccaga tattcgttat aaaaaacagc cggatgaatt agcgaattta ccaaaaatcc
    29401 aattggaaat tttagaaagc tgcgcaccaa ctttaaagtc ttccggtatt ctgacttata
    29461 gcacttgtac gatcttaaaa gaagaaaacc aagacgttgt ttctgctttc ttggaaagac
    29521 accctgattt tgagaggatt gatgttttga ccaatcctat gctacaatca tcaatcgaag
    29581 agaaaatgtt gactttgtat ccgcatcagt tttacacaga tggatttttc atttgttgtt
    29641 tgcgcaaaaa gtaatgaggt gaaaaaatgc agattgaata tcaatcagat gtaggaagac
    29701 gacgtaacac aaatcaagac tatgccagtg tatttaccag ccgttcaggt ctcaagcttg
    29761 ctgtattagc agacgggatg ggtggtcatc gtgccggaga cattgccagt cagatggcgg
    29821 taacgaatct aggcgaagct tgggaaaaac aagaagctgt tgacgatgaa aaaatcgccc
    29881 aatggttcat tcaatcgatt caagaagaaa atatgctgat ttatcagcgt ggacaagaac
    29941 agccggaata taacggtatg ggaacaacaa tcgtagcggc cgctctgtca gatgactggt
    30001 tcacgattgc caatgttggg gatagcagag cttatttgat tcgtgaagat aaaatcatgc
    30061 aattgacgga ggatcattct ttagtcaatg aactagtcaa atcaggagaa atcagcgaag
    30121 aaatggcagt gaaccatcca aggaaaaata ttttaacacg ttctgtcggc atgcctggaa
    30181 cagtcgaagt agatgtttct actcatcttt ggcaactaaa agatcgactg ctgttatgtt
    30241 cagatggctt gacgaacatg ctgtctgaag aagaaatagg aacgatcatc aatcaagaag
    30301 gcccattgtc tcataaagta actgagctga ttgatcgtgc caatgaagca ggcggagcgg
    30361 ataatatcac cgttcttctt atcgaatata aggaggacag tgtatgatcg agttgggcaa
    30421 aaaattaaat ggccgttatc aaatcactgg aaatatcggt agcggtggta tggcaaatgt
    30481 atttttggcg cacgatttaa tcttggatcg tgatgtagca gttaaagtct tacgatttga
    30541 ctttcaaaat gaccagactg cgattcgacg atttcagaga gaagctttag cagcaacaga
    30601 actcgttcat ccaaacattg ttagtgttta cgatgttggt gaagaagaca acatgcagta
    30661 tctggtgatg gaatacgtca aaggaatgga cttgaaacga tatatccaaa cacattatcc
    30721 tgttccttat gaaacagcag taaatattat gcagcagatc ctttctgcta tttctttagc
    30781 tcataaccat caaatcatcc accgagattt gaaaccccaa aacgtactga tcgataatga
    30841 aggtgttgta aaaatcacgg atttcgggat tgctatcgct ttatctgaaa catcgatcac
    30901 gcagacgaat acgatgctag gatctgttca ttatctgtcc cctgagcagg ccagaggaag
    30961 tatggcaacg aagcagtcag atatttatgc tttagggatc atcctttatg agatgttgac
    31021 gggtagcgta ccgtttgatg gtgaatctgc tgtaacaatt gctttaaaac attttcaaga
    31081 tgatcttcca tctataaaag cacttgatcc aaatgtgcca caagcactgg aaaacgtgat
    31141 tttacgggca acggctaaag aacctgcaga ccgttataag tcagccgagg aaatgtctga
    31201 tgatctttct acagtattat caccagctcg tgcaaacgaa gaaaaatggc agcctcatgt
    31261 gatggataat gaaacaaaag ttatcacacc ccttacggaa gatgcaccaa tgcctgattc
    31321 attcaaatct atgcctctgc caaaagacaa gccagaaaac gaaaccttac cagaggagga
    31381 agaagttctg cctgatccta aaaagaaagg acagaaaaaa tggtggctga tcctattagc
    31441 tattcttgct gttttaggaa tcggaacagc tgtattcttt gcttctggcg ggcgaggaga
    31501 agttgttgtt cctgatgttt ctgatctttc tgaagcttct gctagagaaa cactcgcaaa
    31561 agcgaaatta gaagtagcaa caaaaacaga agaaattgca gatgacaaaa tcgaagaagg
    31621 taatgtagtg aagacagacc cggcagcggg aacgacagtc aagcaaaaac gcgaagtcac
    31681 tctgtatatt agtagcggca cgaaaaaaat caaattagat gattacagtg gtaaaagtta
    31741 tgaagatgcc gaagcaagtt tgaaagaatt agggttttct gctgacttga ttcaaaaaga
    31801 agaggaattc agttcagagg tagaaagcgg aatgatcatt tcgcaatctc catcagaaga
    31861 tacagaagtc gatcctaaaa cagacacgat tactttcaca gtcagcaaag gaccagaaac
    31921 agtgactttg agtagtcatg taggagaaaa ctacgaaact gttcgtaatt ggcttgttac
    31981 acaaggattc aatatcaata atattagtgc tacttatgac tatagcgaca ctgtaccatc
    32041 aggttcgatc atcagtcagg atccagcctc gggtgaagtt gtagcagaag aaacttacat
    32101 caattttgtc gtaagccaag gaacagagcc taaattgaat gatatctccg gctatacaaa
    32161 aagcgaagca caaagttatt tagctagtgt cggtgcagaa tatatcgggc atgagacttc
    32221 cgaatattca aacacggtgg ataaagataa agtgattcgt acagtaccag gggcaggaac
    32281 gacaattacc aaaggaatgg ttgtgaacgt aatttactct aaaggaccag atccagcgtc
    32341 ttctagttct gaaaaagact cttcagacag ttctagcagc tcaagtagct ccagtagcac
    32401 aagcaactct tcaaaagaag aatcttccag ctccacaagt tcatcagcta gcacctcaga
    32461 aagttcaaaa taagtgctga ataaaatata tcagaaggga atgcgacatt gatttgttgc
    32521 attctctttt ttgtgtttct atctcttttg atcaattttc aagccatata ctttgagaag
    32581 tttgcatgga ataacaccta ctttcctctt cgtatggtaa aataaacaaa aacggaggtg
    32641 gcacttatca aaggacaaat cagaaaagca atcagcggtt tttactatgt atacgcaaac
    32701 ggagagacct atcaaacaag aggaagagga aatttccgga atcggaaaat cacgccgctt
    32761 gtcggagatc aggtgatctt tgagagtgaa aatcaaacag acggctatct tctggaaatc
    32821 ttgccgagaa aaaatcaatt agttcgccca ccagtcacaa atgttgatct tggaatcatt
    32881 gtcacaagta tcgtagagcc tgcattttct tataatctac ttgaccgctt tctagttacc
    32941 ttggaatacg agtcgatcga accgattatt tttttgacaa agaccgactt gtcagacgac
    33001 ccagaaaaaa tcgaagagat caagcaaacc tatactaaaa taggctatcc ggtgattgta
    33061 cccaatcata cgggtgatac gacagaactg atccgttact tcccagaacg gctatcggtc
    33121 tttatgggtc agtctggagc aggaaaatcg acgctactga atcaaatatc accggaactg
    33181 aatttagcaa caggagagat ttctgatgca cttggaagag gacggcatac aacacgtcat
    33241 gtggaactga ttcctatcta tggcgggtta gtagcagata caccaggatt tagctcgatt
    33301 gactttttga caattgaagc aggagaactg ccgagacaat ttcctgaatt tgtagaagct
    33361 tctgtccatt gcaaatttag agagtgcatg catgctaaag aacctggctg cgaagtaaaa
    33421 catagagtag aaacaggtgg tatcgcacag acacgttacg acaattattt gcaattctta
    33481 caagaaatcg aaaaaagaaa accaatgtat gcaaaaaaag aaggtaaacg aaaataggag
    33541 gaaacaagaa tgaagatagc accatccatt ttgagtgcag attttgccaa tttacaaaga
    33601 gacattgagt tagtggaaaa aggaggcgca gattacatcc acgtagatgt aatggatggc
    33661 caatttgtcc caaatatcac attagggcca aatattgttc aggcaatccg tcctgttact
    33721 aaattaccgt tagacgtcca tctgatgatc gttgatccag aaaactacat tccagcattt
    33781 gcaaaagctg gagcagatat cattactgtc cacgtcgaag caactcctca tattcatcgg
    33841 gcacttcaaa tgacaaaaag cttaggagtt aaagctggag tagttatcaa tccaggaaca
    33901 ccagtctcca tgatcaaaca tgttttgcct ttggctgatc aagtactagt catgactgtc
    33961 aatcctgggt tcggcggaca aagctttatt gaagaaacag tagaaaaaat tacggaactc
    34021 gctgatctac gtagagagaa caattggact tatgcaatcg aagtagatgg cggaatcgtt
    34081 ccagaaacag caaaaatctg taaagatgca ggggcggatg tcttcgttgc aggatcatat
    34141 atctatggag ccgaaaatcc agtcggacaa atcgaacgat taaaagaggc attagactaa
    34201 atgaaaattt tattagctgc tggaggtaat ccgcaaaact ggccgcagtc tctgctgtcc
    34261 aaatacgatg cctatgtcgg gatagaccgt ggtgcgctgt acctgcttca agcaggctac
    34321 ccagtagatc tggcagtggg cgattttgat tcattgtcga acgaagagca gaaggaagta
    34381 tttgcaaaag ccaaagagta tacccgttcc cctgcagaaa aagacgatac agatacacag
    34441 ttaggtatct taaaaacatt tgaacgttac ccgcaagcgg aagtcacttt gattggagct
    34501 acgggcggaa gattggatca tcttttagcc aatctctggt taggtatgga acctcgcttc
    34561 aaaccctttg cccatcagct tattatcaga gattgtcaaa attatttgtc ttattatttg
    34621 cctggagagc atatagtaag acagatacca gatatgaagt atttagctta ttgctgcttg
    34681 actcccgtct cgaatcttac attaaaaggc agcaagtatg tattggaaaa taaagaagta
    34741 gaatatccga catcttatgc aagtaacgaa tttctttcag ataccgcaga attttctttt
    34801 tcagatggta tgattgcagt tattcaaagc aaagattgat ccataaacaa ataaaaaaat
    34861 ttgtctttca ggctaaaaat gacagaatct attatttgct aaaaaaaagc ttaagaggag
    34921 caacttacct tttgtttcac ggcatttagt gataaaattg aacacaagag tctaattaat
    34981 tcgaggtgtc tttgtgaaga agttcaatcc taataaaaat attattatta ccttggttat
    35041 tgttatcatc gtggtgacga tactaagtgt gaccgctgcg cagcgagcga atgaaggaaa
    35101 gcccaatttc ttccaatcca tcgttaatga cagtgttgca atggttgata aaacaatctc
    35161 tgccccagta cggtgggtac gaaatggagt agattctgtc cataatcttt tttcaactta
    35221 tgccgaaaat gagcggctaa aagaaaaaat cgatagctat gatgacgttt cacagcaaaa
    35281 caagaattac gagagagaaa tcgaagcgtt gaaaaaagaa cttgagttga atcagacgct
    35341 gactagttac gaaaaagtaa cggcaaatgt cattactcgt tcgcctgaca gttggcagga
    35401 catgctgatc gtagacaagg gttctaaaga cggtatcgaa gcaaatatgg cagtcatgtc
    35461 gcaaaaagga ttgattggcc gagtgatcga ggtcaatacg gcttcgtcta aaatcgaatt
    35521 actgtcatcc tctaatgaaa gctccaatca ttttccagta cgggtatctt cggctaatgg
    35581 cgaagcgttt ggtttgctta aaaactatga tgaaaagctc catgccttag tggtgaccca
    35641 attaactggt gatacggata tcaaagaagg ggatgttgtc cagacatccg gtcttggagg
    35701 gaattctcca gctaacttgc cgatcggtac ggttattaaa acgaaaccag atagttatgg
    35761 gctggatcgg gaagtttatg tgaaacctta tgcagaaatg tatgacgtgt cagttgtgac
    35821 gattgtccaa cggctagcgg aggattagtc catgttgaag aaagaaacaa tgaagtacta
    35881 tctgccaatc gttttgttct ttttgatgtt gatagatggt catttaacaa gaatgctagg
    35941 ggagtggtcg aaaggcacct atatgtcaaa tgcccacttt ctgatattgg cattattatg
    36001 ttgcagtatg gcgtttgaaa aacgttattt actgattacc acgattgttc tcggggctat
    36061 ctatgacgct tactatattg gcgttatcgg tatctatgca gtagctctcc ctttaattgt
    36121 atggttgatg tatgtaatga aagacgttat ccatgtcaac atctttactg aatttttcag
    36181 tatgatcatc tttgtcacgg gttatgaatt gtttacgatg gtggtccagt tgatttttaa
    36241 attagcagta gtaaataaca cgtattttat tacaaggttt ttaggaccta cactgctgtt
    36301 gaacatgatt atatttgtat tattcatttt tccctttaag aaattattca gtgatgaata
    36361 atttcttttt ttatttttag gtcgaaagcg aaaaagaatc gttgttatct taagaaatgc
    36421 tagacttaac gcggcttttt catgatcatt ttctgaaacg aacagttttg tcatttttaa
    36481 aagttgtttt gaaacatttt tgtaacataa ctattatatc gttgacacac tgctatggta
    36541 caatttttat atcgttaaga aagacttaaa tatatttaga tatttattaa attaagccag
    36601 tcggaggaat gaagagtgaa aaagagttta atatcagcag taatggtatg ttcaatgacc
    36661 ttgactgccg tagcatcacc gatcgctgcc gcggcggacg attttgattc tcagatacaa
    36721 cagcaagatc aaaagattgc agacttgaaa aatcaacaag cagatgcaca atcacaaatc
    36781 gatgccttag aaagccaagt ttctgaaatc aacacacaag cacaagacct attagcaaaa
    36841 caagatacat tgcgtcaaga atctgcacaa ttagtaaaag acattgcaga tttacaagaa
    36901 cgtattgaaa aacgtgaaga tacaatccaa aaacaagcac gcgaagcaca agtaagcaac
    36961 acaagctcaa actacatcga tgctgtattg aatgctgatt cattagcaga tgctattgga
    37021 cgcgttcaag caatgacaac gatggtaaaa gcaaacaacg acttgatgga acaacaaaaa
    37081 caagataaaa aagctgtaga agacaaaaaa gctgaaaacg atgctaaact aaaagaatta
    37141 gctgaaaacc aagctgcttt ggaatctcaa aaaggcgatc ttttatctaa acaagctgat
    37201 ttgaacgtac tgaaaacttc attagctgct gaacaagcaa ctgctgaaga caagaaagct
    37261 gacttgaacc gtcaaaaagc agaagctgaa gcagagcaag ctcgtattcg tgaacaacaa
    37321 cgtctagcag aacaagctcg tcaacaagct gcacaagaaa aagctgaaaa agaagcacgc
    37381 gaacaagcag aggctgaagc acaagctact caagcttcat ctactgcaca aagctcagca
    37441 acagaagaat catcagctac acaatcttca atgactgaag aatcatcatc agctacacaa
    37501 tcttcagca
//
LOCUS       DACECR010000016        37213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.016, whole genome shotgun sequence.
ACCESSION   DACECR010000016 DACECR010000000
VERSION     DACECR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.016"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(45..779)
                     /locus_tag="IWO17_000760"
     CDS             complement(45..779)
                     /locus_tag="IWO17_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0289657.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="IWO17_000761"
     CDS             1106..1516
                     /locus_tag="IWO17_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ0289658.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="IWO17_000762"
     CDS             1509..2210
                     /locus_tag="IWO17_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ0289659.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3796
                     /locus_tag="IWO17_000763"
     CDS             2210..3796
                     /locus_tag="IWO17_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0289660.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3815..4036
                     /locus_tag="IWO17_000764"
     CDS             3815..4036
                     /locus_tag="IWO17_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ0289661.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4033..4794
                     /locus_tag="IWO17_000765"
     CDS             4033..4794
                     /locus_tag="IWO17_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ0289662.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5174..5683
                     /locus_tag="IWO17_000766"
     CDS             5174..5683
                     /locus_tag="IWO17_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0289663.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5758..6297)
                     /locus_tag="IWO17_000767"
     CDS             complement(5758..6297)
                     /locus_tag="IWO17_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ0289664.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IWO17_000768"
     CDS             complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IWO17_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ0289665.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7364..8161
                     /locus_tag="IWO17_000769"
     CDS             7364..8161
                     /locus_tag="IWO17_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ0289666.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8189..8824
                     /locus_tag="IWO17_000770"
     CDS             8189..8824
                     /locus_tag="IWO17_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ0289667.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8844..9617
                     /locus_tag="IWO17_000771"
     CDS             8844..9617
                     /locus_tag="IWO17_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0289668.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10025..10822
                     /locus_tag="IWO17_000772"
     CDS             10025..10822
                     /locus_tag="IWO17_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ0289669.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10800..11213
                     /locus_tag="IWO17_000773"
     CDS             10800..11213
                     /locus_tag="IWO17_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289670.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11197..13842
                     /gene="mutS"
                     /locus_tag="IWO17_000774"
     CDS             11197..13842
                     /gene="mutS"
                     /locus_tag="IWO17_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0289671.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13860..15968
                     /gene="mutL"
                     /locus_tag="IWO17_000775"
     CDS             13860..15968
                     /gene="mutL"
                     /locus_tag="IWO17_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ0289672.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15990..16550
                     /locus_tag="IWO17_000776"
     CDS             15990..16550
                     /locus_tag="IWO17_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ0289673.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16635..18623)
                     /gene="treP"
                     /locus_tag="IWO17_000777"
     CDS             complement(16635..18623)
                     /gene="treP"
                     /locus_tag="IWO17_000777"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ0289674.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18846..19559
                     /gene="treR"
                     /locus_tag="IWO17_000778"
     CDS             18846..19559
                     /gene="treR"
                     /locus_tag="IWO17_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ0289675.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19636..20082
                     /gene="msrB"
                     /locus_tag="IWO17_000779"
     CDS             19636..20082
                     /gene="msrB"
                     /locus_tag="IWO17_000779"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ0289676.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20252..20854
                     /gene="ruvA"
                     /locus_tag="IWO17_000780"
     CDS             20252..20854
                     /gene="ruvA"
                     /locus_tag="IWO17_000780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ0289677.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20867..21868
                     /gene="ruvB"
                     /locus_tag="IWO17_000781"
     CDS             20867..21868
                     /gene="ruvB"
                     /locus_tag="IWO17_000781"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ0289678.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21897..22475
                     /locus_tag="IWO17_000782"
     CDS             21897..22475
                     /locus_tag="IWO17_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0289679.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22527..23009
                     /locus_tag="IWO17_000783"
     CDS             22527..23009
                     /locus_tag="IWO17_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ0289680.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23126..23500
                     /locus_tag="IWO17_000784"
     CDS             23126..23500
                     /locus_tag="IWO17_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289681.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24178..25530)
                     /gene="brnQ"
                     /locus_tag="IWO17_000785"
     CDS             complement(24178..25530)
                     /gene="brnQ"
                     /locus_tag="IWO17_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0289682.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25677..26267
                     /locus_tag="IWO17_000786"
     CDS             25677..26267
                     /locus_tag="IWO17_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0289683.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26395..27744)
                     /locus_tag="IWO17_000787"
     CDS             complement(26395..27744)
                     /locus_tag="IWO17_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0289684.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27912..28652)
                     /locus_tag="IWO17_000788"
     CDS             complement(27912..28652)
                     /locus_tag="IWO17_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0289685.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28665..30257)
                     /locus_tag="IWO17_000789"
     CDS             complement(28665..30257)
                     /locus_tag="IWO17_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0289686.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30825..31121)
                     /locus_tag="IWO17_000790"
     CDS             complement(30825..31121)
                     /locus_tag="IWO17_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289687.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31297..32022
                     /locus_tag="IWO17_000791"
     CDS             31297..32022
                     /locus_tag="IWO17_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ0289688.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32015..32857
                     /locus_tag="IWO17_000792"
     CDS             32015..32857
                     /locus_tag="IWO17_000792"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ0289689.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32860..33381
                     /locus_tag="IWO17_000793"
     CDS             32860..33381
                     /locus_tag="IWO17_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ0289690.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33634..34197)
                     /locus_tag="IWO17_000794"
     CDS             complement(33634..34197)
                     /locus_tag="IWO17_000794"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0289691.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34448..34717
                     /gene="rpsO"
                     /locus_tag="IWO17_000795"
     CDS             34448..34717
                     /gene="rpsO"
                     /locus_tag="IWO17_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ0289692.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34905..37031
                     /gene="pnp"
                     /locus_tag="IWO17_000796"
     CDS             34905..37031
                     /gene="pnp"
                     /locus_tag="IWO17_000796"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ0289693.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
BASE COUNT        12130 a         6922 c         7760 g        10401 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca aaagcaatcg
     2101 agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac ggaatattga
     2161 ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgactgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaacaaaa
     3781 actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa ttttaccaga
     3841 attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac tttatgttgt
     3901 tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat tgatcaaaac
     3961 aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag cagatgattg
     4021 ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt atttatcaca
     4081 ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca agatgctttt
     4141 gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg atttcctgaa
     4201 acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca agcggtatcg
     4261 aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg gatattggat
     4321 gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta tgagacgaat
     4381 gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg ccaaaaaagc
     4441 ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg tgggatcgct
     4501 tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct ggattacgca
     4561 ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc aatgaatgcg
     4621 gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac accagtcaag
     4681 cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc tcctcaagca
     4741 agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa ataaccttgt
     4801 gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg atgataaaaa
     4861 aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa ttctttttaa
     4921 taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa acgatattca
     4981 aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag ctatttttat
     5041 ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc gacacttcag
     5101 ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt atcttgcaaa
     5161 ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga caacgacaga
     5221 tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg ttttgtctgt
     5281 ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc tgtccttgta
     5341 taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata ttgcttctcc
     5401 tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat tgcttatcaa
     5461 ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga cagcatgcgt
     5521 atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc aactaccttt
     5581 tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg tcggtgggat
     5641 aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg tttaatttaa
     5701 aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct tttttgatta
     5761 atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat aagctgctcc
     5821 agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa atgcagttgt
     5881 aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg cagcaagaga
     5941 gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt cgatcatcca
     6001 accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg gcccaaataa
     6061 aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc caagtaaaag
     6121 ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg ttaatccaat
     6181 agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct gagaaaggac
     6241 agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc ggttcataac
     6301 gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa acaaagtgat
     6361 tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc ggtctttttc
     6421 aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga gcttcgtcga
     6481 tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc aatttttcag
     6541 cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct tttactgtta
     6601 cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg tggtatgaag
     6661 ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat tgacgtgctt
     6721 gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt tgttctagta
     6781 agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt acgaacaagt
     6841 tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt tcagttaatt
     6901 gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct ggtgcgtaag
     6961 ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga cgagaaattt
     7021 tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat aagtttttgg
     7081 agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat cttcaccttt
     7141 gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat gagttattat
     7201 ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca cgaatgtcaa
     7261 gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa tgagagggta
     7321 tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc cggtatcacc
     7381 attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg aaaacagctt
     7441 ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag gaacggctgt
     7501 ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa agattttata
     7561 cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg aattaggaac
     7621 atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt taagtaaggc
     7681 agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat tatgtggatt
     7741 tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta tgttagaatc
     7801 aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga tgtttgcgaa
     7861 tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg tcggaaaagt
     7921 gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac acgtgatagc
     7981 gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg atggaatgac
     8041 agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata ttggtggcgg
     8101 tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct atgtggacta
     8161 aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa aaaatgagga
     8221 aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg gagtacctaa
     8281 gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt tatctatttc
     8341 tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt atcagtatgt
     8401 agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact tagtcgtatt
     8461 gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga gaaaagtata
     8521 cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat tgtttgaaaa
     8581 aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt ataaacagca
     8641 aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa aggaattctt
     8701 tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg ccgattgggt
     8761 aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa gtaagtattt
     8821 ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat attgatggaa
     8881 cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg gaaaaattac
     8941 gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat gcgcaagaga
     9001 tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc ggttttttag
     9061 accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg ttcgttcaag
     9121 aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc aagcgttctt
     9181 cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct gtcttgcctg
     9241 aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca tttttcgatc
     9301 aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc tggcatgaga
     9361 acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg cacttagcag
     9421 aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa aacgaccgtg
     9481 aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca gaagtacaag
     9541 cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag gcattgaaag
     9601 agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt aataagccca
     9661 gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc gcagatgcaa
     9721 gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc aagaaataag
     9781 aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat ttctgaagaa
     9841 gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg tcatagtccc
     9901 atttttttat tttatttttc aaaactaaga aataccagga aagagttcct tttgtttgac
     9961 cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag aagaggaatt
    10021 tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag agatgattac
    10081 tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg taaatggtga
    10141 gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt tgcaagacgg
    10201 cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct ttgagtttgt
    10261 aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc ctggacaagg
    10321 gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc aggctcgttc
    10381 ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag aagaagccag
    10441 aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg aaaaacaagc
    10501 aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata cacacgttca
    10561 gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg ttgggatgac
    10621 aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa agtttctgac
    10681 tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa gcggctgtat
    10741 cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc agatcaacga
    10801 tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag ccggaaattc
    10861 aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc attcgctaca
    10921 aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga gaaaaaatca
    10981 aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca gcagaaaaag
    11041 aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg cttgttgtag
    11101 cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca actatgatac
    11161 aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac taaaaatact
    11221 cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt tttgttctat
    11281 cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc acagcttttg
    11341 gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat gtgcggtgta
    11401 cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta taaagtagcg
    11461 atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg cgaagtgatc
    11521 cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa agacaacaat
    11581 tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc cgatttaagt
    11641 acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa tgaagcctct
    11701 gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct tcgtgaacag
    11761 ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga aaacgcggaa
    11821 tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac cggcaaacta
    11881 ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa agcagtagaa
    11941 taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct agaattaagc
    12001 caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga tgaaacaaaa
    12061 acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat ccaggcaaaa
    12121 caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt tgaacgtatc
    12181 gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg tcgagtagct
    12241 ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga acaagtgcca
    12301 atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct gctttcagaa
    12361 atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga tgatccgccg
    12421 ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct cgatatgtac
    12481 cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa agagcgtcaa
    12541 gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta ttatatagaa
    12601 attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa acaaacttta
    12661 gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca aatcctcgaa
    12721 gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag agaagaagtc
    12781 aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt agatgtgcta
    12841 caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact tgtcagtgac
    12901 aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt attaggacat
    12961 caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt gctgattaca
    13021 ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac tgttctgatg
    13081 gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt tgatcggatt
    13141 tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt tatggtagag
    13201 atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat tttgtttgac
    13261 gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc aatcatcgaa
    13321 tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca tgaactgacg
    13381 gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt tgaaaaagac
    13441 ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag ctacggcata
    13501 catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc agatatcctg
    13561 catacgctag aaaatggcga taacggacac catactgttc cagctgaagt agaagagaaa
    13621 actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa tcaagaccat
    13681 atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga attatccgaa
    13741 aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat gacgccaatg
    13801 gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg cggatatcga
    13861 tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg ggagaagtag
    13921 tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac gccggcagta
    13981 ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg attgataacg
    14041 gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg acaagtaaga
    14101 tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg gaagctttgc
    14161 caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa gaaaaacaag
    14221 gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca ccactgcgtc
    14281 aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc ttgaaatacg
    14341 tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga ctggcattga
    14401 gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg gcaactgctg
    14461 gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc gcaaaaaaaa
    14521 tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt tcattgcctg
    14581 aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt tttatcaaga
    14641 acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg gtcggtcgtt
    14701 tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta aatgttcatc
    14761 cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta attagtaacg
    14821 ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat aatctacggt
    14881 ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat ttgtcctacg
    14941 cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga gagacaggta
    15001 cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat aagtctgtgg
    15061 aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca gaaaaaacag
    15121 cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa acagtggaaa
    15181 aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat gaagaacaag
    15241 cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca ctccatcctg
    15301 aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag aaattagaaa
    15361 cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg cacggaacgt
    15421 atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct gcccaagagc
    15481 gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat ctgcaagaat
    15541 tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta aaagagcaaa
    15601 aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac agctttattg
    15661 tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt cgggagatga
    15721 tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa gcaacagcaa
    15781 tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa gcacaagcac
    15841 gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc catggccgtc
    15901 cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga atccaagacc
    15961 cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc caatcaccaa
    16021 gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct gctgatatcg
    16081 atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg gctgaagaaa
    16141 aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt gccagtgaca
    16201 cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct gaggctgaat
    16261 tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt gttttaaaaa
    16321 caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat cctttgacag
    16381 acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca ggggcatacg
    16441 gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat tacagtatcg
    16501 taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa acaaaaaagc
    16561 ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt gaaaagatta
    16621 cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc cgggcgaaca
    16681 agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa agcaacaata
    16741 atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc aatcagggtc
    16801 tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc ctttagttca
    16861 acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag aataccaatg
    16921 gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata gactttgccg
    16981 tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac tgggtcattt
    17041 acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc ttttgttgca
    17101 actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt tgcgccattt
    17161 gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat cttattcaaa
    17221 attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc aatgatcatt
    17281 gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg caaaccgcct
    17341 actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc taatccagag
    17401 ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa catagcaggt
    17461 tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc ttctttttta
    17521 ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat atttgataaa
    17581 gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat atcgatggca
    17641 ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc gaatacggca
    17701 ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga gatccagctt
    17761 ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt tggtaacaat
    17821 gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg gaagaagatt
    17881 tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc ttgataacca
    17941 atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc ggctgtggaa
    18001 gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa aacgattcct
    18061 aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac tggaagaaaa
    18121 tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca aaacttcgag
    18181 tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac atttctgaat
    18241 ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat ttctgctaaa
    18301 acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt tccttgttct
    18361 ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt agaaacatcg
    18421 tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac gctaggtata
    18481 tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg catacgagtc
    18541 gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa cagcttctcg
    18601 gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg tctatgcaag
    18661 ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt attaaaaagt
    18721 acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa aaataggaac
    18781 attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg agtgagggaa
    18841 aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt ttagcgaaag
    18901 aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt tatggtgttt
    18961 cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat attcaaaaaa
    19021 aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct atttctggat
    19081 tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg atcatccata
    19141 cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg gagatcggtg
    19201 cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt atattagaca
    19261 ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca caaggatcga
    19321 tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa aaggagatca
    19381 cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat gacacttatg
    19441 cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag tattctgagt
    19501 cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga aaagtgtaaa
    19561 tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga taggatcaca
    19621 aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact gaaagaaaag
    19681 ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc atttagcggg
    19741 aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg tgaaccgtta
    19801 ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag caaaccaata
    19861 acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg aatagaagtt
    19921 cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc agtagatcaa
    19981 ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata tgaaaagatg
    20041 gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata cacaaagagc
    20101 ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg attgctattt
    20161 gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca ataaagggaa
    20221 cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag gtgtggttac
    20281 ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc agatcgcact
    20341 aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt atgtccatca
    20401 ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag aaaagcaact
    20461 gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg caatcatggc
    20521 aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca cttatttgac
    20581 gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc taaaaggaaa
    20641 attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa acacaaaaga
    20701 cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca gtgatcgaga
    20761 aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg atgagtatct
    20821 acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga cagatgaata
    20881 cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc ttcgaccaca
    20941 gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga tttacatcga
    21001 agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc ctcctggatt
    21061 aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata ttcgtacgac
    21121 gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg aactggaagc
    21181 aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag aagagatgct
    21241 ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc caaccgcaca
    21301 tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac gggcaggtat
    21361 gctttctgca cctttacgag atcgatttgg catcatttct catatggaat attatgacga
    21421 acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag agatcattga
    21481 agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg ctaatcgctt
    21541 gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg atcgtcctat
    21601 agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt atgttgacca
    21661 gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt taagtacgct
    21721 ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac cgtatctgat
    21781 ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt atgcctatga
    21841 acattttggt tacgactatc atatatgatt tttgctttta gattggagga aaatgaatga
    21901 gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg tgccaaacgc
    21961 aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc aatcgacaaa
    22021 cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat tgtgaggatg
    22081 cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag caagcattga
    22141 aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg ctttctgacc
    22201 cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc aagatttttg
    22261 aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat gctcgttttt
    22321 tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat gaagaaacac
    22381 cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta tattcttatc
    22441 gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg ttggaaaagc
    22501 aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt ttgggaaata
    22561 tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta gaaggaaggg
    22621 aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga aagacaccgc
    22681 accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat atggttgctc
    22741 ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg gatcaagaaa
    22801 atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc catttgtttt
    22861 taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat accaataact
    22921 ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat atttggattg
    22981 atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag gcgatgttag
    23041 agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa gtaacacttt
    23101 cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa tgaagaagct
    23161 tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa atgacactga
    23221 agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc aaaaagctgc
    23281 tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta gcaagtatga
    23341 aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga tgatccgcct
    23401 acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa aaataacaat
    23461 cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag atagaaaagc
    23521 acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc tttaagctga
    23581 tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt gggttgatta
    23641 gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct agccggggag
    23701 ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt tggtatgctt
    23761 atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt tgttaagttt
    23821 ttttgcgaaa actattttta tatcatatat atgtctaaaa tctatattat aaaagaaatg
    23881 ttgtttgatc agcttctctt cggtattagt tcaaaacaaa acggcaaagt atatggagct
    23941 actttgccgt tttgtttctc taatactcag gtcagtttat agacgctcaa taaacggtgt
    24001 tcaaaagcca gtttctcggt attaagtcaa atttatgcaa aaatgtgaga agccattttc
    24061 aaaaaatatt tcttaatact cgaaactgag cggctttttc acgacctctc aataaacagt
    24121 gttcaagaga cagcctatcg gcaataaggc tgaacgtctc tttcacaacc tcttttatca
    24181 atctgctact tcttctttct tagcgaaact ccaaatcagt ccgataacaa aaccgataac
    24241 tgctggtacg atccacccca tccccaattt gaaaaacggc aagtagcttt ccgcaacata
    24301 taaaagattt tgaacaatag gtgtttgttt gatagtttct gggcaagcgt tcaacccatc
    24361 aaaaatcgaa gcaatcaatg taaaataagt agtcattcga taaacagccg tacgctgctt
    24421 aaacaaagga ccaactaaca ctagtaaaat cagtgtcatt gctaatggat agatgaacat
    24481 caaaacaggc aacgaaaatt gaatgatatt cgtcaagcct acgtttgccg ctaaacaagg
    24541 aaggatacta gcaagtgcaa caaagaatgc gtaatttctt tgcggaaaca aatctgtgaa
    24601 tgtttctgca aaagctgtaa tcaaaccaat accggtcttc aagcaagcta atataacgat
    24661 aaatgctaat aagatactgc cataagtacc taaataatga tcagcaatct gagccagcgt
    24721 gatcccgcca ttttcactta gagcaaagct acctaagctc atagcaccta aataagcgag
    24781 caaagtataa atgattccca tcaatataat gctgatcgca ccagatttga tcgtgtcttt
    24841 agcgatttca gcaggttttg ttacacccat gttttggatt gttgtgacaa tgatgatccc
    24901 aaatgctaaa gcagcaagcg catctaatgt gttatatcct tgagtaaacc ctgtgaaaaa
    24961 agcatgctct tgataatttg gctgaatagg cgcttgattt acagaaccta gtggattcaa
    25021 aaaagcaagg agaagcaaag cacctaataa aaccaaaaat gcaggattca ggaactttcc
    25081 aacataatct aatatctttg tcggttttct tgaaaaccac caagccagca caaaaaatac
    25141 aatactgaaa attgctagat atagcgtctg tctgctagga tcgataaatg gtgctaaacc
    25201 aatctcgaaa gatgtggttg caaggcgagg taaagcaaaa aacggtccga taactaagta
    25261 aagcaagatg gtaaaaacca tcgcatatgt ttgatttatc cgttttgcta attcatatac
    25321 acctgagctt ttcgatacac caatggcaat cacccctaag aagggaagac cgataccggt
    25381 aaccaagaaa cctagattcg cccaaaaggt agcagcgcct gcttcttgac ccaaatgaac
    25441 gggaaagata agatttccag caccaaaaaa tagaccgaaa agcatggagc ctataaaaag
    25501 atagtcttta aatgataatt tttttgtcat acataactcc tttacggttt ttttaaaagt
    25561 aacaaaattt cgaaaaatat gcaacaattt tcagaaaatt tcctatataa aacgacggtt
    25621 atttaataac gtaagtgata aaataaaagc gctgaatcat ctgaggagga atcatcgtga
    25681 caaagtcgag attagaagca tttactgatg gagtggtagc cattgttttg agagttcttg
    25741 tattagagat caagatccct aatacatcta gctttcattc tttatgggtc atccgaaata
    25801 cattattagc ttataccatc agctttatat ttgttgcggt gatctgggtg aaccatcatc
    25861 gtatatttca gatggcagaa cgaattaatt accgagtggt ctggtcgaat atctttttgc
    25921 tcttttggct gactttatgt ccttctgtta ctagttgggt cgggcgtaac cctgaagctt
    25981 tttggcctga gatggcttat gtcttagtat acacgatgtg gagtttttct tttggtatct
    26041 tgatgaggca aatcatgaaa gcaaataaac cagattccca tgtcgtgaga gttttgtcaa
    26101 aggaccgtcg aagtcttatc tccatgatga ttaacttatg cttgattgcc ggtgtctttt
    26161 ttgtaccgtt gatcgggtta ttcggacgtt tttttgtatc tggtatttgg attttttcct
    26221 ataaaaaagc agatgcctat tatcagcggt tgtttccggg gaaatagaaa agaggctggg
    26281 acagaagctt ttaactccaa gaaataagcc gaaatttcac aaaaatttaa gaagcaattt
    26341 tcgtaaattc cttcttattt ctcgaagctg accacttctg tcacaactac tttcttactt
    26401 cttacgttca tatttcatat gatagattag taatagaagc aagaaccaca caggtgttag
    26461 taataaagcg attctcgtat cttttcctag gaacaagacg acacacacaa aggccatgta
    26521 aagaaatacc agataattac taaatgggta caacggcatt ttaaatgaat gagctttttt
    26581 gccgaggata cttttacgat atttcatgtg cgtgtagaca agcatactcc agataaacaa
    26641 gaaacaagta gtggcaatac tgcttactag tgtgaatact tcactaggca tcacgtaatt
    26701 caagataaca gaaatgaaaa tgacgatagt agaaaatgtc aaagcattcc ctggcacatg
    26761 atgtgttgtt aactttttga attttttagg ggcactgcct tcttgcgcta aagaacgaag
    26821 catacgtcct gtactatata ttgcactgtt gcaggcagaa gctgctgcac ttaatacgac
    26881 taaattgatc aaggaagcag caatcgtgat cccaatttcc gaaaatactt ctacaaatgg
    26941 actgttatct ggattcaagc tgttccatgg atagattgcc ataatgacaa aaagagatcc
    27001 taagtaaaat aagatgatcc tgataggaat attattgata gcttccggca acactttttc
    27061 aggattttct gtttctccag cgactaatcc aactaattct atcccagcaa aagcaaaggt
    27121 taccatttgg aaagctaaga taaatccttt tgcaccagtt ggaaagaatc cgccatgaga
    27181 ccatagattc gtgatactgg ctgtgccaat atctgtcgta tagtgggtga ggatcatata
    27241 agctcctacg aggataaagg caatgatcgc tacaactttg atcaatgcaa accaaaactc
    27301 taactcacca aacaatgaga cagccaccat attcaagcaa agaagaagac ctaatgcaat
    27361 cagttcagga agccactgag gaacaccagg cgcccagtag cgtacataca ttccgatggc
    27421 tgttaaatcg gccattgcaa tcgataccca gcagaaccaa tacgtccagc cagtgatgaa
    27481 agccgctttt ttgccaagat attctgcaac aaaatccaga aaagaatggt tattggtgtt
    27541 tgataacaga agttcgccta acgcacgcat aatgaaaaaa cagatgcatc cagtaataag
    27601 ataggcgagc aatatcgaag ggccagctag ctggatagac tttccggaac ctaaaaacaa
    27661 ccccgttcca atcgctccgc caatagaaat cagttggaca tgtctatttt tcagtccacg
    27721 ctgcagttct tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt ttaattactt
    27781 ccatattaaa agagttttct gaaaaacaca actaagattc caaaattttc agaaatttac
    27841 cttttatgag taattcataa aaaagaactg cggtaaacac cgcagctctt tgtcccaatc
    27901 agctattgat cctaagcatt taaaatacgt gaaaggaatt cttttgtacg ttcttctttc
    27961 ggatggacaa agatatcttc tggtgtacct tcttctgcaa tcacaccttt atccataaaa
    28021 atgacacggt cagatacatc ttttgcaaat tccatctcat gcgtcacgat aagcattgtc
    28081 aagccagtat gtgctaagtc tttgatcgta ttaagcactt cgccaaccat ttcagggtct
    28141 aacgccgaag taggttcgtc gaatagcaat acatctggat tcattgataa tgcccgagca
    28201 attgcgacac gctgtttctg tccaccagat aattgtgctg gtttggcttc gatataacga
    28261 tccataccaa ctttttccag attttcgatt gctacttttt tggcagtttc tttatctcgt
    28321 ttcaggacag ttgtttgacc agacatacag ttttccaaga cattcatatt gttgaacagg
    28381 ttgaaggatt ggaaaaccat acctagattc gttcgatatt taggtaaatt atatttagga
    28441 gctaatacat tttctccatt ataaatgatc tcgccgcctg ttggtttttc cagtaaattg
    28501 atacagcgca agaaagtaga tttaccagaa ccggaagaac cgatgatcgt cacaacttca
    28561 cctttattga ctgttacgct gatatctttc aatacttcat tttcaccgaa gctttttctt
    28621 agatgtttga tttcaataat tttttcattc atgattcttc ctccttattc ctgcaaattt
    28681 gaattgtcga tttcttcaag cttggtataa gctgaaggac catcgatttt cttttctacc
    28741 gcccgcagga ttctggtgat cgtaaaggtc atgactaagt agatgatccc gacgatcgta
    28801 aatgtttgga agaattggaa attcgcaccg gaagcagagt ttccttggaa gaacaaatca
    28861 gctacgccga ttacacttaa aactgctgta tctttgatat tgataacaaa ctcatttcct
    28921 gttgctggca agatattccg taaaacttgt ggaataacaa cttttctcat cgtttggcca
    28981 tgggtcatac caatggcttg cgcagcttcg aactgccctt catctactgc aaaaatcccc
    29041 ccacgaacga tctctgtcat gtaagctcca gtattgagag agacaataaa caaagcggca
    29101 atcgttcggt tcaatgaaat gccaaaagcg agtgccagac cgtagaagat gaccattgct
    29161 tgtaccatca tcggtgttcc gcggaatact tcgacataga tagaaagaag tgcatttccg
    29221 attttttgga agaagcgagt cagtgcattt cctgattcag gtaccgaacg gaagacacca
    29281 atcagtaatc caagtgtagt accgacaact gtcccgatca atgcgatgaa caaggtaagt
    29341 ccagcacctc gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa atcagaaatg
    29401 atgccaggtt tttcttcact atcagttgca gcaggctgat ctttgactgc ttggtccata
    29461 attttggttc gttcatcgtt agagatgcca gaaagaatct ggtttacttt ttggatatcc
    29521 gggtcgcctt tacgcatacc aacagaaatc tgaacatctt ccgggtttgt ctggaagcca
    29581 tctgcaccgc tgaattcaag catttccaag tctttgttca cactagttgc cgttacacct
    29641 tccggacgtt cgctgacata accatcgatc attccagaag ctaaagctgt tctcattgct
    29701 gagaaattat ccattgcttg ttgtttctgc acatctggta tctgatcgat cacagtatag
    29761 tggaatgtat ttaactgagc agtaatcttt gctccagaaa gatctgctaa actttttgct
    29821 tttgcatatt tgctatcttt ttgaactact actactaatt gtgattcgta ataaggatcg
    29881 gtaaaatcaa tttcttttcg tcgttcagca gtagggctca ttccggcgat gatcgcgtcg
    29941 atcttgcctg attgaagggc aggagccaga ccatcccatt tcgtttggac gatgactaat
    30001 tttttcccta aaccatctgc tactttttta gcgatttgaa catcataacc gccggcgtaa
    30061 ctattttggc cttgaattgg caaggcatta ttctcatctg tttgttgaga ccagttgaag
    30121 ggtgcatacc cagcctccat acctacgcga aactcatcgc tcggtgtttt ttcatctgca
    30181 tttgccagag atcccacact gaatagtgag aagacaaaag caaatagtac ggcaactgaa
    30241 accatatttt ttttcatttt tttctccctt tctgattata tgatacacga gcggtctaga
    30301 tgaaatgcgg taaagcttta tctgaaaaaa taaaaagcga ccgatttata gatataaaaa
    30361 ggccgctaaa attccataag gtgataaacc tctcacaaaa catagcgcaa cttagcatca
    30421 ctgctaagac agtccgcaag ctcttaactt gcgtcccaac atacactcca agcaaaaaag
    30481 tgcacatttc ggcgatcctc cctattcctg cttctccatg tttgcttcac tccacagggt
    30541 taatgattct cgcgacctct acctcattga ttgaggcatc tcgtattcag ttatttatac
    30601 aaaaaaagtt tacgatactc gagaggcttt gtcaatctat aactgctgtt ttttgaaaaa
    30661 atactgataa atatagaaat ctttactttt accgaaaaaa caatcaaaat gtgaatcatc
    30721 tatccattgc aaacgatagt ataattatac acaaaaatgt atgtaataca ataaaaaaag
    30781 aactagaaca gaaaatttcc tgtgtccagt tccatctcga tcatttaagc tgccgctttc
    30841 tttggctcac gtcctaggat cgcttgcaaa agaacaacga cgccataaat ggctaatatt
    30901 agataaatca aacttgagta ttgggtgatt ccccagcccc atgttcgata tacggctaat
    30961 gtaatggcta aaacaattgc ggcaatcacg ttgaataaag caaaagccca aagatttcca
    31021 ttttctttgc gtacaagata aaaaacagag aaataaacgc ttaacgctaa ccaggcgata
    31081 caagccagaa taacgcccca ataaataaag aatggtaaca tatcgattca cctcactttt
    31141 atacagcagt attgtaacac gttttcattc ttttgtcagt gaacattgtg ataaattgtt
    31201 caaatgccca taacactttt ttattcccat ttttctgact tttcaacaat aaatttgttc
    31261 gagtatgggc tataatggaa agtgaggtga caaaacatga aacaattaac agtagacgaa
    31321 gcagcaaaag agatattagc ttatcagcaa attacatttt tgaccggtgc aggggtatcc
    31381 acaccttctg gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg gatggagcag
    31441 ccggaatact tgctgagtca tcaagcaatg gtgaatgaac cggataaatt ctatcaattt
    31501 gtcaaaaaga tttatcatcc tgaagcaaaa cccaatatca ttcatttgga aatggcacat
    31561 ctagcgaaag aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt gcacgcaaag
    31621 gctaaaagcc cgcaactcgt caattttcat ggaagtttgt atcaatgtca ttgtcgaaaa
    31681 tgtcaacaaa cggtagactg gaaagaatat cttcacagcg acaaacatca agcatgcgga
    31741 ggacaaatac gcccagacat cgtgttatat ggtgaagggt ttcaagatgg agtcatggag
    31801 caggcggctt atgccgtcag ccaagcagag ctgatcgtca ttgttggaac gagttttcaa
    31861 gttcatccgt tttgtgatct ggtacagttt cgccagccgt cagccaaaat cttagtaatc
    31921 aatcaaacgc ctgtttattt agcggagcct tatacgttta ttcaagaaga cggagcggtt
    31981 gttttcaaaa aaatacaaga attaggagca gattatgact gaattcaaaa ccgaaaaaga
    32041 aaaaatgatt gcaggcgagc tttattttgc caatgatcct gagctggttg ccgaccgtat
    32101 gtttgcaaga agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac tgcgatccca
    32161 attgttgaaa gagacttttg gccgtacagg gaaaaaaatc tatatggagc cagttatcaa
    32221 ttttgattac ggatataata tatttgttgg ggagaatttt tatgcaaatt tcaattgtac
    32281 atttttagat gtcagcacga ttgaaatcgg tgataactgc atgttcgctc cgaatgtcca
    32341 gctttacacg gcgacgcatc cactacatcc agtgaaacgt aacagcggtc tggagtacgc
    32401 aaaacccatt aaaatcggta ataatgtttg gttaggcgga ggagttatcg tcacgccggg
    32461 tgtcactcta ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca aatcttttcc
    32521 cgacaatgta gtgattgcag gaaatcctgc tcgtatcatc aagacagtag aggaagaact
    32581 aacagaagag tctcttgaaa cattacgtca taagatcgat atgatcgatc gagagttgat
    32641 tgctttatta gaaaaaagaa tggatacggt aactaaaata ggacaagtga aaaaagaaga
    32701 aaaccaagca gtttatgatg caaaaagaga gcagcaagta ttggataaag ttgttagtct
    32761 gctgaaaaat aagggatata aggaaacaat aacagatacg tatgttgatt tgatgaaaca
    32821 ctcgcgcgaa taccaaaaca aaatgaagga ggaataagtg tgatcgaagg agagacacgg
    32881 cgaaaacaaa tcatgcaaac cttggcaaat acagaaaaac cagtgagtgc tagcaagttt
    32941 gcgaaaactt ttggagtcag ccgtcagatc attgtaggcg atattgcttt actacgagct
    33001 gcaggtaaaa gtatcgtggc aacagcaaga ggttatatcc tcgaaacaga agaaaccaat
    33061 actgggcaca tcagtaaaat cgctgtccag catggaaaag atcaaacaga agaagaactg
    33121 agactaatcg ttgaaaatgg tggtgaaatc attgatgtaa ttgtggaaca tcccctttac
    33181 ggtgagttag caggaacttt gcacatcaaa acgccacaag atatcgattc ctttatgaga
    33241 cgttatcaaa aaagtaatgc tactttgtta tccgaattaa caggcggcat ccatctgcat
    33301 acgatccgct acccagaaaa gcatatgctg aaaaacatca aacaaaaact agctgaagca
    33361 ggaatcctct acgaaggata acatcgtagc tgctactttt tatcctagaa actattctat
    33421 catctctgag ataaacaccc atatggaaga aataagaaaa aataagaaaa gatgcgtatc
    33481 caatacacag aattttttgg aattgcgatt tctagatgtc tatctaaatt tcgattagtg
    33541 gagaaacata aaaaagggaa gtgcagcttt tgagcactcc ccttttttat aaagctttct
    33601 tgtagcatag tatcgaagga acctgccaat tctctactcg atcaccaata cgccttcttt
    33661 tagtgcaaat ggattttgat cgttgatgtg gtcatagaac atcacaccgt tgatatggtc
    33721 gatttcatgt tgaacgacga tcgattcata attcttcaaa cgaatcttgt gtttttcacc
    33781 attcatatca taataagaca cagtgatttt ggcatggcga acaacatatc ctggtacttc
    33841 tcgatcaaca gataaacagc cttcaccttc ccctaaacaa gcatcttgaa cagaatggct
    33901 caaaatttta ggattataca taacagtact tagagaaggt ccatctgcct ctgggtcagg
    33961 acttggtacg tgaacagcaa tgattctttt agaaatatct aattgaggag cagctaagcc
    34021 aacaccgcct cgtaagtgta gttcttcagc tttgattgga tcttggctgt tttttaagaa
    34081 ctcaagcatc tcttttccta aagagatatc ttcttcgctt aatggaagag aaacttcttt
    34141 tgcaacttcg cgtaaggtcg gatttccttc acgaatgata tcatccattg taatcatgga
    34201 atatgtaccc ctttcttaaa agactgtgtt tcaagtctat tttcgcatta tagtttacca
    34261 caaaaatgga aaagtagtag taagattgtt tgaaaactac cttttcttaa tgattaacta
    34321 cttgattttc aacccggttt caggtaaagt atagctgtgt gctctaggca catgacctgc
    34381 cacttggtta agcgaatcac caacgtttta ctcagtcgca ggggaataat cgaaagaggt
    34441 gaaagaaatg gcaatttcaa aagaacgcaa gaatgaaatc atcaacgaat acgctcgcca
    34501 tgaaggggat actggttctc cagaagtaca aatcgctgta ttaactgaag aaatcaacca
    34561 cttgaatgaa catgcacgcg tgcacaaaaa agaccatcat tcataccgcg gcctaatgaa
    34621 aaaagttggt caccgccgta acttgttagc ttacttacgt aaaactgacg ttcaacgtta
    34681 ccgcgaattg atcaaacgtt taggcttgcg tcgttaattt tagtcaaaaa gagtgggatt
    34741 cacatgaatc tcactttttt tactcgtata tcaattaagt aaaattccgt gatcaagcta
    34801 gatggactcc tactaaccaa atgaaagctg ctcaaaagac ggctttcggc agttttcatt
    34861 tgtttagtag cgtcttgctt tcacgagaag gaaaaggaga agttatgtca gaaaaacaag
    34921 tattcaaaac gacatggggc ggccgtccct tacaaatcga agtcggtcag ctagcaaaac
    34981 aagcaaacgg agccgtttta gtccgttatg gagatacagt agtcttaagt gcagctgtgg
    35041 cctctaaaga agcaaaagat acagactttt tcccattaac gatcaattat gaagaaaaaa
    35101 tgtatgcagc agggaaaatc cctggaggtt tcatcaaacg agaaggccgt ccaagtacgg
    35161 aagcaacatt gactgcccgt ttgatcgacc gtccaattcg cccgatgttt gcagaaggct
    35221 tccgtaatga agtccaagta acgaacatcg tgatgagtgt agaaacagac tgctcgccag
    35281 ctatggcagc aatgctaggt tcttctttag cactatcaat ctcagatatc ccatttgacg
    35341 gcccaatcgc aggcgtagaa gttggtcgtg taaatggcga gtacgtcttg aatccaacag
    35401 tagaacaagc agaacaaaca gatatcgaat tgactgttgc cggaacgaaa caagccatca
    35461 atatggttga aagcggcgcg aaagaagttt cagaagaaga catgcttggt gcattgctgt
    35521 ttggttttga cgctatcaaa gagttagttg ctttccaaga agaaatcgtt caagcagtag
    35581 ggaaagaaaa aatggaagtg actttactgc aagttgatga agtattgaaa aaagaaatct
    35641 ttgatgcttc ttacgctaca atgaaagcag cagtaatgac tgaagaaaaa cttgctcgcg
    35701 aagataatat cgagcaggtg aagatcgata tccgcgaagc ttatgctgag aaatttgcag
    35761 gtcatgaaga tgaagatcac ttgttgaaag aagttaaaca aatcacagaa gatcttgaaa
    35821 aagatgtcgt acgtgaattg atcaccatcg ataagatccg tcctgacggg cgtaaattgg
    35881 atgaaatccg tccattgtca tcagaggtca gcttattgcc acgtgtacat ggatcaggtt
    35941 tgttcacacg aggacaaaca caagctttat cagcttgtac actcgcacct ctaggagagc
    36001 accaaatcat tgatggttta ggggtggaag tcagcaaacg attcatccac cactacaatt
    36061 tccctcaatt ttctgttggt tctacaggac gtgcaggctc tccagggcgt cgggaaatcg
    36121 gacatggcgc attaggtgaa cgtgcgttag cacaagtaat cccttcagaa gaagaatttc
    36181 cttatacgat ccgtttagta gcagaagtat tggaatcaaa tggttcttct tcccaagcaa
    36241 gtatctgtgc tggcacatta gcattgatgg atgcaggtgt accaatcaaa gcgccagtcg
    36301 ccggtattgc gatgggactt gtttctgatg gtgaaaacta tacgatccta actgatatcc
    36361 aagggctgga agatcatctt ggcgatatgg actttaaagt tgccggtaca aaagatggta
    36421 tcactgcttt acagatggat atcaagattc aaggaatcac agaacaaatc ctaactgaag
    36481 cattaactca agcaaaacaa gcacgtatgg aaatcttaga agaattgact tcaacgattg
    36541 ctgctccacg tgaagaatta agccaatatg caccaaaaat cgaaatgatc cagatcgaac
    36601 ctgctaaaat caaagatgtt atcggtaaag gcggcgatac gatcaacgga atcatcgatg
    36661 aaactggtgt gaaaatcgat atcgatcaag atggaaaagt aagtattgct tcagctgatg
    36721 cagaaatgat caaaaaagct atcaagatca tcgaagattt gaccaaagaa gtcaaagttg
    36781 gtgaagttta tctagggaaa gttgtccgta tcgaaaaatt cggtgcattc gtcaacttga
    36841 tcaaaggaaa agatggactt gtccatattt cccaattagc aaacgatcgt gtcaataaag
    36901 tcgaagatgt tgttaaacta ggtgatgaga tccttgtgaa agtaactgaa atcgataaac
    36961 aaggacgcgt aaatctgtca agaaaagcaa tgttgaacga agatggttca acgaaaaatg
    37021 aagaaaaata atttaattca taggaataat gtacttgtga tcgtgaaggt gacaggggga
    37081 caataattat tcgaaaaaag gggactgtga caaaagccgt gtcacagtcc ttttttttcg
    37141 aataaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt taaaaacatt
    37201 aggagctgtt ttt
//
LOCUS       DACECR010000017        36230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.017, whole genome shotgun sequence.
ACCESSION   DACECR010000017 DACECR010000000
VERSION     DACECR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.017"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            232..1500
                     /locus_tag="IWO17_000797"
     CDS             232..1500
                     /locus_tag="IWO17_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAQ0289694.1"
                     /translation="MGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKSVEEIPSPVT
                     GTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETSGSASVVEAA
                     DPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPSSAPAKSEAP
                     EAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVNSKHTAPHVT
                     LHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLNASIDDANQE
                     IVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDGKLSAEDMRN
                     GTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGRVMKLSLSFD
                     HRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1508..2914
                     /gene="lpdA"
                     /locus_tag="IWO17_000798"
     CDS             1508..2914
                     /gene="lpdA"
                     /locus_tag="IWO17_000798"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ0289695.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            3076..3351
                     /locus_tag="IWO17_000799"
     CDS             3076..3351
                     /locus_tag="IWO17_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ0289696.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3357..4124
                     /locus_tag="IWO17_000800"
     CDS             3357..4124
                     /locus_tag="IWO17_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ0289697.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            4292..6121
                     /gene="typA"
                     /locus_tag="IWO17_000801"
     CDS             4292..6121
                     /gene="typA"
                     /locus_tag="IWO17_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ0289698.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6382..8154
                     /gene="recQ"
                     /locus_tag="IWO17_000802"
     CDS             6382..8154
                     /gene="recQ"
                     /locus_tag="IWO17_000802"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ0289699.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            8268..8570
                     /locus_tag="IWO17_000803"
     CDS             8268..8570
                     /locus_tag="IWO17_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ0289700.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8573..9736
                     /locus_tag="IWO17_000804"
     CDS             8573..9736
                     /locus_tag="IWO17_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0289701.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9762..13190
                     /locus_tag="IWO17_000805"
     CDS             9762..13190
                     /locus_tag="IWO17_000805"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ0289702.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            13211..14335
                     /locus_tag="IWO17_000806"
     CDS             13211..14335
                     /locus_tag="IWO17_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289703.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            14325..14762
                     /locus_tag="IWO17_000807"
     CDS             14325..14762
                     /locus_tag="IWO17_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0289704.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14746..15084
                     /locus_tag="IWO17_000808"
     CDS             14746..15084
                     /locus_tag="IWO17_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ0289705.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            15203..15763
                     /gene="rsmD"
                     /locus_tag="IWO17_000809"
     CDS             15203..15763
                     /gene="rsmD"
                     /locus_tag="IWO17_000809"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ0289706.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15760..16251
                     /gene="coaD"
                     /locus_tag="IWO17_000810"
     CDS             15760..16251
                     /gene="coaD"
                     /locus_tag="IWO17_000810"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ0289707.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            16241..17284
                     /locus_tag="IWO17_000811"
     CDS             16241..17284
                     /locus_tag="IWO17_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0289708.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17443..18171
                     /locus_tag="IWO17_000812"
     CDS             17443..18171
                     /locus_tag="IWO17_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ0289709.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            18287..18532
                     /locus_tag="IWO17_000813"
     CDS             18287..18532
                     /locus_tag="IWO17_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0289710.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18529..18747
                     /locus_tag="IWO17_000814"
     CDS             18529..18747
                     /locus_tag="IWO17_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ0289711.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18883..19422
                     /locus_tag="IWO17_000815"
     CDS             18883..19422
                     /locus_tag="IWO17_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ0289712.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19415..21688
                     /locus_tag="IWO17_000816"
     CDS             19415..21688
                     /locus_tag="IWO17_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ0289713.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21726..22772
                     /gene="holA"
                     /locus_tag="IWO17_000817"
     CDS             21726..22772
                     /gene="holA"
                     /locus_tag="IWO17_000817"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ0289714.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22861..23115)
                     /locus_tag="IWO17_000818"
     CDS             complement(22861..23115)
                     /locus_tag="IWO17_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ0289715.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23515..23784)
                     /locus_tag="IWO17_000819"
     CDS             complement(23515..23784)
                     /locus_tag="IWO17_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289716.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(24254..24709)
                     /locus_tag="IWO17_000820"
     CDS             complement(24254..24709)
                     /locus_tag="IWO17_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ0289717.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24847..26775)
                     /locus_tag="IWO17_000821"
     CDS             complement(24847..26775)
                     /locus_tag="IWO17_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0289718.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26972..27805)
                     /locus_tag="IWO17_000822"
     CDS             complement(26972..27805)
                     /locus_tag="IWO17_000822"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ0289719.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27997..28701)
                     /locus_tag="IWO17_000823"
     CDS             complement(27997..28701)
                     /locus_tag="IWO17_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0289720.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28724..29350)
                     /locus_tag="IWO17_000824"
     CDS             complement(28724..29350)
                     /locus_tag="IWO17_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0289721.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29676..32051
                     /locus_tag="IWO17_000825"
     CDS             29676..32051
                     /locus_tag="IWO17_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ0289722.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(32075..32566)
                     /locus_tag="IWO17_000826"
     CDS             complement(32075..32566)
                     /locus_tag="IWO17_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ0289723.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32753..33673
                     /locus_tag="IWO17_000827"
     CDS             32753..33673
                     /locus_tag="IWO17_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0289724.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33827..34171
                     /locus_tag="IWO17_000828"
     CDS             33827..34171
                     /locus_tag="IWO17_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ0289725.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34361..34861)
                     /locus_tag="IWO17_000829"
     CDS             complement(34361..34861)
                     /locus_tag="IWO17_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0289726.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35389..35472
                     /locus_tag="IWO17_000830"
     tRNA            35389..35472
                     /locus_tag="IWO17_000830"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35423..35425,aa:Leu,seq:cag)
     gene            complement(35796..>36230)
                     /locus_tag="IWO17_000831"
     CDS             complement(35796..>36230)
                     /locus_tag="IWO17_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ0289727.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11990 a         6327 c         7491 g        10422 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa
      241 ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa
      301 gatgatacat tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc
      361 acagggacag ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta
      421 ttagtagaaa tcgatgctcc tggacacaat agtgcaccgt caactagtgc gccatcagct
      481 gaagcaccaa aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat
      541 ccaaataaac gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta
      601 gatatctcac aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa
      661 aacttcttgg caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca
      721 gcaccgaaag aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc
      781 aagtccaact taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc
      841 gctaaagcaa tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta
      901 gaagtgtcta aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt
      961 acgaaactta ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac
     1021 ccagtattga atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac
     1081 aatatcggta ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca
     1141 gaccgcaaag gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat
     1201 gacggcaaac tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga
     1261 tctgtaggtg gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt
     1321 gtaggaacga ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt
     1381 gtgatgaaac tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa
     1441 gcaatgaata atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga
     1501 taaacaaatg gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg
     1561 acctggtggc tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat
     1621 tgaaagagag tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact
     1681 gatagctgca ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag
     1741 tgaaaacgtc aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca
     1801 tacgttgact tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg
     1861 agaagcgttc ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac
     1921 ttattcgttc aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt
     1981 taaattcgga ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa
     2041 gaaatttgtg atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa
     2101 cttaggagca gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa
     2161 agatctagta aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa
     2221 tgcaatggcg aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga
     2281 cgggaaagaa gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa
     2341 tacagatgat atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac
     2401 ggtggataaa caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc
     2461 aggggcggca ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc
     2521 tggcaaaaaa gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga
     2581 attggcttct gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc
     2641 atataaattc ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt
     2701 catccgtcta gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt
     2761 cggcgcaagt gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga
     2821 agatatcgcc ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc
     2881 ggaattagca ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac
     2941 tgaaaaatca gttgcgtttt gttttttaga attatcaata atattattat gtaaacttaa
     3001 aaatatgaca acttcttggt ttattaccac ttcctatgct acactgaaga ggaatatgaa
     3061 ataggagagt tgaagatgca agaatatcag tatccacttg atttagattg gacaacagac
     3121 gaaatggtca ttgtaatgaa gatgtgggaa gatctggaac gtgcgaatga aaaagggata
     3181 aacaatgaag cttttttgaa gacttatcag cagtttaaaa ctgttgtcaa atccattggt
     3241 gaggaaaaac gattgggacg ggaatttgaa aaagcttccg gttattcttt atatcgtaca
     3301 gtgcagcaag caaaaaaaaa tccaaacaaa actttgagaa tgaacgggtg agataaatgg
     3361 aacagatgat tgatcatatc aagtcctggc ttgaagaagc tggtgagaaa atcaaatctt
     3421 ctcaacaaac attaacaatc tcgcaaaaat caaatcgaac ggatctagta acaaatgtcg
     3481 atcgaaacat ccagaatttt ctaatcgaaa aaatccattc ctttgatcca gaggcgaaaa
     3541 tattggcaga agaagaaggg tgtaatcaat tatcggatct cgatggaaga gtctttatca
     3601 tcgatcctat tgatggcacc ttgaattttg tgattgaagg ggaaaatttt tgtattatgc
     3661 tcgctgttta tgaatccggc gttggaaagc ttggtttcat ttatgacgtg atgagaaatg
     3721 aacttttttg gggtggaaaa ggaataggcg tgtataaaaa tgatgagcca ttgcctagac
     3781 cagcaaataa atccttatcg gaaggcttac ttggtgtgaa tagttatctt ttcggtcata
     3841 ataaatatgg aatccgagaa attgcggaaa gcagcatggg gacacgtatg tacggatgcg
     3901 ctggtcttga actagtggca atcttgaaag gaaaccacat tgcttatatt tcaaacctta
     3961 gcccttggga ttatgcagcg ggaaatgtct tattggaaga gtttggtatg gtaacaagcg
     4021 gtctttttgg tactcctttg acatttgaaa aaagggaata ctatttagca gggacttctt
     4081 gtgcatatgc agaaatcgca gaccttttga aaaaagaagg ctgaaaaaaa ggtagaaatc
     4141 attcacaaaa atgataaaaa cgagcaaaag attaattttc tcgaaaaaat gaaaacattt
     4201 tactatattt catgaaaaaa gtctgctata attagcagtc gagtaaaatt tgccctgtca
     4261 taaagaacca ataaaaaagg agttagttac attgaattat agaaacgata tccgtaacgt
     4321 cgcaattatt gcccacgttg accacggaaa aacaacatta gttgatgaac tattaaaaca
     4381 atcagataca ttagacgcac atacgcaatt gcaagaacgt gcgatggact caaatgcgct
     4441 agaaaaagaa cgtggtatca cgattttagc caaaaacaca gctgtagact ataaaggtat
     4501 tcgcgtaaac atcatggata ctccaggaca cgcggacttc ggtggcgaag tagaacggat
     4561 catgaaaatg gttgacggcg ttgtgttggt cgtagatgcg tatgaaggaa caatgcctca
     4621 aacacgtttt gtattgaaaa aagcactgga acaacatatc acaccaatcg ttgtcgtaaa
     4681 caagattgac aaaccttctg ctcgtcctga gcatgtcgta gatgaagttc ttgaactatt
     4741 catcgaatta ggagcagatg atgatcagtt agactttcct gtgatttatg catctgcact
     4801 aaatggtacg tcaagcttgt cagatgatcc agcagatcaa gaaccaacca tggcacctat
     4861 ttttgataca attattgaaa aaatccctgc tcctgtcgat aacagcgacg aaccattaca
     4921 attccaagtt tcattacttg actacaacga ttatgtaggt cgtatcggaa tcggacgtgt
     4981 atttagagga acaatcaaag taggagacca agtagcatta attaaattag acggaacagt
     5041 taagaaattc cgtgtaacga aattatttgg tttctttgga ctaaaacgtc tagagattca
     5101 agaagcaaaa gctggagact tgattgctgt ctcaggtatg gaagatatct ttgtcggtga
     5161 aacagttact cctgtggatc atcaagatgc gttacctatc cttcacattg acgaaccaac
     5221 attgcaaatg actttcttag ttaataattc accatttgcc ggtcgagaag gtaaattcgt
     5281 aaccgctcgt aagattgaag aacgtttgat ggcagaactt caaacagatg tatccttacg
     5341 agttgaacca acaaactctc cagatgcatg gacagtttct ggtcgtggag aattgcattt
     5401 gtctatcttg attgaaaaca tgcgccgtga gggatacgaa ttgcaagtat ctcgtccaga
     5461 agttattgaa aaagaaatcg acggtgtaaa atgcgaaccg tttgaacgcg tgcaaatcga
     5521 tacaccagaa gaatatatgg gtagtgttat cgagtccttg agcttacgta aaggtgaaat
     5581 gcaagatatg gtccatacag gaaacggaca aatccgtttg acattcttaa cacctgcccg
     5641 cggtttgatt ggttattcaa ctgaattcct ttcaatgaca cgtggttacg gaatcatgaa
     5701 ccatactttt gaccaatatc tgccaatgct tcctggtcaa attggcggac gtcaccaagg
     5761 ggcgcttgtt tcgatcgata caggaaaagc aacgacttat tctatcatga gtatcgaaga
     5821 acgcggtaca gtcttcgtag aaccaggtac agaagtatat gaaggaatga ttatcgggga
     5881 aaacagccgt gataatgatt tgactgtcaa catcacgaaa gcaaaacaaa tgacaaacgt
     5941 acggtctgct actaaagacc aaacatctgt catcaaaaaa ccaaaacaat tgacgttgga
     6001 agaatctttg gaattcttga atgatgatga atattgcgaa gtaacaccag aaagcattcg
     6061 cttgagaaaa caaattttag agaaaaatgc tcgtgaaaaa gcatctaaaa agaaaaaata
     6121 atctgtaaaa ggatgaagag agaaaccagc ttgttataaa aaatagctgg tttctttttt
     6181 tgtccaactc ttttctgtgg agaagtcatc tttgtcaatg gacataaatt cagaaaatac
     6241 gccgaaagac gtttaaaaga cttttaaaaa acttatttct agagagtttt tgtttcataa
     6301 taagataatg tttcacgaaa ggagatcaac ttgtccttcg aagaaaatcg aagtaaaatg
     6361 gcaagagaaa ggagcgggaa gatgaacgac ttattaaaac aatactttgg ctatgatgag
     6421 tttagacctg gtcaaaaaga aattatccag aaagtcattg atcaggaaaa tgtattagga
     6481 atcatgccta ccggaagtgg gaaatcaatc tgttaccagc ttccagcttt actattagat
     6541 gggcttactg tggttgtgtc ccctttaatc tctttgatga aggatcaggt ggacgcggcg
     6601 aatcaattag gtatacccgc cacttttatc aatagctcgt tagatggtta cgaaacagct
     6661 agacgcttcc aagaaatcga tagacagcag taccgtttac tttatatcgc tcctgaacgt
     6721 tttatcatgc cggattttat ccaagcaatg aagcgatgga acgtccgtat gatcgctatt
     6781 gacgaggcac attgtatctc tcagtggggg catgatttcc gcccaagtta tttacagatg
     6841 gcgaaccagc tagatcaact gccaaatcgt cctgtaattg ttgctttgac agctacagcg
     6901 actgtgcaag tagctgctga cataaaaaga cttttaaaaa ttccagaaaa caaccatatt
     6961 cagactggtt ttgaacgaga aaatctacga tttcaagtaa ttaaagatca aaaaaaagaa
     7021 caatatctaa tcgaatattt aaagatcaac aaaaatcaat ctggtatcat ttatgcagca
     7081 acgagaaaag aagtagaccg tctctatcat ttgcttaaaa aattcgattt ttccgtgggt
     7141 agatatcatg gtggtctaaa tgagaatgag cgaacagaaa tgcaagaagc ctttttatac
     7201 gatcgcttgc agctgattgt agcgacaaat gcatttggta tgggtataaa taaaagcaat
     7261 gtccgttttg ttatccatta tcaaattcct ggctctctag aagcatacta tcaggaagct
     7321 ggtcgggcgg gaagagatgg tctttccagt gaagccatcc tgcttttctc tcctcaagat
     7381 atccaagtcc aaaagttttt tatccagcag tcccaaagag aagaagggca aaagcaaaaa
     7441 gaatatgaaa aattaaaagc gatgacagaa tacgtgtata ttgagtcctg tcttcagcaa
     7501 tacatcctaa actattttgg cgagacaagc agtccgtgca accgttgtgg taactgttta
     7561 gatgacagag aaatagtaga agtaacgaca gaagcacaga tggttctttc ttgcttaaaa
     7621 cgtatgggtg agaactatgg taaacaaatg ctgatgaaag tcttagcagg atcgaaagaa
     7681 caaaagctgc aagctttagg ttttggacat ttatctacct atggactgat gaaaaaccaa
     7741 tcgcaaaaag aaacgatgca gttaattgaa tatttgatat cgaatggcta tcttctgacc
     7801 atcaatggtg agtatcctgt tttgaaagta acagaaagag ggatacaagt attgaaagga
     7861 caagaatctg tttatcgaaa ggaacccaag aaagtccagc agctatcgga tgaagaaacc
     7921 gatacactat ttgaagtgct gcgggaactg cgaacggatc tagcatcaga agcaggcgtt
     7981 cccccgtatg tcgtattttc agattccaca ttgaaagaaa tgagccgaat acgtccatca
     8041 agcagattgg agatgcttca gataaaagga gttggccaaa gcaagcttga taaatatggc
     8101 gaagcctttt tatctcggat aaaaaatgcg gactctaacg ttctggataa gtagaaagaa
     8161 cgaatacgta aaaatgaata aaaacaactg gttaaatctg ttttttttgc tccttatgaa
     8221 aatcaaatat gttataatta aggaattaga atgggaggta agcacagatg gattctttgt
     8281 cgaaaaaact tgcgatccaa gtattaaatg atgaagcaga acggatcaaa cgattaatcc
     8341 gaaatcaaaa caacagtctc tgtatctcac aatgcaaagc cttcgaagaa gtggtagata
     8401 cacagatgta cggcttttcc cggcaagtcg gttttgctgt ccgtatagga gtaatcggaa
     8461 agaacgaggg ccagctgatg ttaagtgagc tagaacgaga attgaatcag ttatatagtg
     8521 aggtttacca agaaatttat gataaaaaag aagttggcaa ggaggaatag acttgcctaa
     8581 gaaagtgaaa aaaaggcatc tattagacta tagcatcttg attccctatt taattttatg
     8641 tgtattggga ttgatcatgg tatatagttc tacgtcgtat ctattattag aaaacggaca
     8701 aaatccgtcc gcatcagtca ttaaccagag tattttttgg gtgcttagct tgattgctat
     8761 cgcactactt tataaaatga aaacagatgt tttgaaaaac cagcgtttga tcatggcagc
     8821 catcgcagtt ttgacgattt tgctgctgat tgttgtgttt tttggtaagg agatcaatgg
     8881 ggcgaaaggt tggctgcaaa ttgcaggatt ctcgatccaa cctgccgaat acctaaaaat
     8941 catttctatc tggtacctgt ctctgacttt atctaaaaga caaaacagtg tacaaaaaga
     9001 ttttcttggg actgtgaaac gtccacttgc aatggttatc ggacttactg cattagtcgc
     9061 tattcttccc gattttggta atgcggcagt tatctttttg attattttgg ttctgctttt
     9121 agccagcgga gtcaattatg tgtatacttt gattgtagga gtaggaggct tttgtctaag
     9181 tacttttacg atctggctga tcaatatcac aaacggaaag atcttacccg gacgtttaca
     9241 gtatatctat aatcgatttg ctatttacca aaatcctttc tcagacgaat taaataaagg
     9301 gcatcaacta gtcaacggct actatgccat gttcaacgga ggactatttg gcaggggatt
     9361 aggtaacagt atccagaaaa aaggattttt acaagaagca caaaccgatt ttatttatgc
     9421 aatcgtagtg gaagaactgg gagtgattat ggggattttg atattagcgt tgctattctt
     9481 tatgattgtg cgcattgtcc ttgtaggtat ccgttcaaaa gatccgttca attcgctttt
     9541 gtgcattggg atcggcgcaa tgtttttgat ccaagtattt gtcaatcttg gaggaattac
     9601 tggcgttatt cctttgacag ggatcacgtt cccattttta agtcaaggag gctccagttt
     9661 gctgatgctt tcgatttgtg tcggctttgt cctcaacatc agtgcggatg aaaaacgcaa
     9721 gagcttaggt ttataataaa aaaagatgta ggagtggttc gatgaaaaaa gttttagttg
     9781 ctaacagggg tgaaatcgct gtacgtgtat ttcgtgcttg tacagaatta ggaattaaaa
     9841 ctgttggaat ctatgcagaa gaagatgaat attccgtaca ccgtttcaaa gcggatgaag
     9901 catatttagt aggacaagga aaaaaaccga ttgatgcgta tctagatatt gaagggatca
     9961 tttctatcgc gaaagaatgc ggagctgacg ctattcatcc gggttacggt cttttgtcgg
    10021 aaaacttgaa ttttgcgcaa cggtgcgaag aagaaggcat catttttgtt ggacccaaac
    10081 tgcatcattt agatattttt ggagataaaa tcaaagcaaa agctgcagct atcgaagcgg
    10141 ggattgcttc gattccaggc acagatggac caatcgcttc tatagacgat gccttggagt
    10201 ttgcgaaaca atacggttat ccgattatga tcaaggcagc tctaggcggc ggcggacgcg
    10261 gcatgcgtgt agctcacgat gaaaagagtg caagagaggg ctacgaacgt gcgaaaagcg
    10321 aagcaaaagc agcctttggt agtgatgaag tgtatgttga aaaatacatc gctaatccta
    10381 agcatatcga agtacagatt ctcggggata cgcatggaaa cgtcatccat ctttttgaaa
    10441 gagattgttc tgttcagcgc cgtcaccaaa aagtagtaga agtagcgcct tgtgtctcta
    10501 tgaatgaaca gcaacgtcaa aaaatctgcc aggcagctgt ccaattgatg aaatatgtcg
    10561 ggtatgtcaa cgcaggtaca gtagaatttc ttgttgaagg agatgatttt tactttatcg
    10621 aagtcaaccc tcgcgtccaa gtggaacata cgatcacaga aatgattaca gatattgata
    10681 tcgtaacaac gcaactgttg attgctcaag gtttggatct gcataaagaa atcggattgc
    10741 ctcagcaaga aggaattaaa ttaaatggtt cagccattca atgccgaatc actacagaag
    10801 acccgttgaa taacttttta ccagacacag ggaaaatcga tacgtatcgt tcacctggtg
    10861 gttttggcgt acgtctggat gtcggaaatg cttacgcggg ctatgtagtg acaccgtatt
    10921 ttgattcatt gctagtaaaa gtatgtacgc atggtgctac ctttgaaaca gccattcaga
    10981 aaatggaacg ttgcttgaaa gaatttagaa tccgtggagt gaaaacaaac attccattta
    11041 tgctcaatgt catcacacat cctgaattcc aatcaggaaa tgcaaaaaca accttcattg
    11101 atagtacagc aacattattt gaatttcctc gactacgaga ccgcggaaac aaaacgatga
    11161 aatatatcgg tgaaattact gtgaatggtt ttccaggtat cgaaagtggc gaaaaaccat
    11221 tttatgaaga accacgtatg cctaaagatc taatcacaag aactgattac gtaacggcta
    11281 agaatgtatt agatgcgaag ggtgccgatg ctttggtcga atggatcaaa ggacaggaaa
    11341 atttgttgtt gacggatacg acatttagag acgcccatca aagtctgcta gcaactcgtg
    11401 ttcggacaaa ggatttcaag cagattgcac gtttgactgg agaaggtctt cctgaactct
    11461 tttcaagtga gatgtggggg ggagcaacat ttgatgtggc ttaccgcttt ttgaatgaag
    11521 atccttggca acggttgaga aaaattcgtt ctttaatgcc taatacgttg ctgcaaatgc
    11581 ttttcagagg ttcaaatgcc gttgggtatt ctaattatcc tgacaatgta ttggtggaat
    11641 ttgtcaaaga agcagcagcg caaggaatcg atgtatttcg aatctttgat agtttgaact
    11701 ggactccaca aatggaaaaa agtatccaag ctgtacggga tactggtaaa atcgcagagg
    11761 cagctatttg ctatactggc gatatcaacg atccaagtcg ggcaaaatac aatgttcagt
    11821 attataaaga catggcaaag gaactagaac aactgggtgc gcatatcatt gcaatcaaag
    11881 atatggctgg gctgcttaaa ccgcaggcag cttatcgttt gattagtgaa ttgaaagaaa
    11941 cgacagatct gccaatccat ctgcatactc atgataccag cgggaatgga atcatcactt
    12001 attctgctgc ttcaaaagca ggagtagaca tcgttgacgt ggcaatgagt gcaatgagtg
    12061 ggaatactag tcagcctagc atgagcagct tgtattatgc actggtcaat ggtccacggc
    12121 tgccagaaat cacgattgaa aatgcacaga aattaaacca ttattgggaa gatgtgcgca
    12181 tgtattacaa accgtttgaa aacggcttga atgctcctga aacagaagta tatatgcatg
    12241 aaatgccagg aggacagtat tcgaatctac agcaacaggc aaaagctgtc ggattaggtc
    12301 atcgctggga tgaaatcaaa caaatgtatc atactgtcaa cttgatgttt ggcgatatcg
    12361 ttaaagtaac tccatcatct aaagtagttg gagatatggc actatttatg gtccaaaatg
    12421 atcttacaga agaagatatt tatgaaaaag gcgaaacact aagtttccct gaatctgttg
    12481 tgactttctt ccaaggagaa ctagggcaac ccgttggcgg atttcctaaa aaattgcaaa
    12541 agattattct taaaggcaga cctgcattga atgaacgtcc aggtcttttg gctgaatcag
    12601 tcgacttcaa cgaagtaaaa aaagaattgg cagaaaaaat tggctatgaa ccaaaacaag
    12661 acgaagtatt aagctatttg atgtatccgc aagtcttttt ggactatcaa aaagcatata
    12721 atcaatttgg cgatgttaca ttactggaca caccaacatt tttccaaggt atacgtttgg
    12781 gtgaaacaat caatgtacaa atcgaacgcg gaaaaacgct tatcattcgt ttagatgaaa
    12841 tcggagaacc cgatatcgaa gggaatcgcg tactgttctt caatttgaat ggacaaagac
    12901 gtgaaatcac tatcaaagat aattcgatca tcagtacggt ccaaacccga ttgaaagctg
    12961 aaccgacaaa ccgagaacaa attggagcaa cgatgtctgg ttctgttttg gatgtattag
    13021 tgaaaaaagg cgataacgtt aaaaaaggtg atacgttgat gattactgaa gcgatgaaaa
    13081 tggaaacggc catcgaagca cgttttgacg gagaagtcgc acatgtatat gtgtcttcag
    13141 gagacactat ttcgtctggt gaccttttga ttgaagtgac tgagaaataa taagcgaaag
    13201 gtggggaaac atgaaaagac tgggtttgtt ttggctgatt ctgctattag ttattgtggc
    13261 attctacatg gaaccagtct tgtttccgcc aaaacttgaa gtgaaggaaa actctgatca
    13321 acaaatatca aaccaaatga caacgtggaa ataccaggag cttaaagcaa ctggtttttc
    13381 tacttatatc ggaaaaccga tcgatcagct ggaaaaggat tttggccaag cctatgatcg
    13441 attaactagc ggatttggtt ttgaaacaag atattatgaa aataaagcta gtcatctcac
    13501 atttgaagcg aacatccaag acagcagagt cacaactgtt aaagtactgc aaacagggcc
    13561 tgctgatatc gcgccgtttt atttagggat gacgatgcaa gatcttacga aaatcactac
    13621 tatctatact aatttcactt ttgagtacca tgatacagaa gttggaatcg aactaatgga
    13681 agaggatatg aattatcgtc cgctaattgc ttttgataac gacacttttg ctattttatt
    13741 ttttgatcaa acaacaaacg gcctattttc agttgtgtat ttagataaag atagtctttt
    13801 gaaactattg ccatatcaag tgtttggtga aggccttcca cattatcaag cagataaatc
    13861 ggctgattgg gaatcaatca atcgcgttaa agaacgaaaa agtacgactt tattaaatat
    13921 gcttagaaga gaagacgaac ttcccatata caatcaaaca cttgcctttc aaaaccacag
    13981 tcagctgcta ctacatgatt ttttgacaag gcctgaggaa atattgtcag aggaacgtat
    14041 ggctgattgg caaaaatcgc taggcagtgc tcaaacgaca gtgaaattct cactgacgaa
    14101 tgatgaattg gagaagttaa tcagtaaaca aaaattagaa aatactagtg ggctatttat
    14161 tcatccggcg attgacgcta ctttttcgtt tttatattgg tacagcgatc cttacaacca
    14221 tgaccgtttc atggacccgg gaacggattc tgtaggcatt gctttttcta aagaaaatat
    14281 gttagtctta ttgcaagaag aaaacggaga aagtagtgat agcagtgatc gttaacgaag
    14341 agctgtttca actggaggat caatgcctcc aagtcgctga aatgataaaa aagagtaaaa
    14401 cgatggcttc ttatcttgaa cataaacagt caatggaaga atctgcaaaa gtttgccagt
    14461 tgaaagaagc gttcttgtct aagaaagaag catttgaaaa tgtagcggca tatggtgagt
    14521 atgcacctga ttatcgtgaa aaaaaacgag ctgttcgaaa agcaaaaaga cagcttgata
    14581 tggatgagca tgttgcagct ttccgatata gtgaaacaca gctgcaaagt ctgttggatc
    14641 aaattgtcca agtacttgcc gaagaagttt ctaatgaaat caaagtagat gcaggtagcc
    14701 cgttttttga aacgaaaaaa cactttggat gcggaggtaa ttgtcatgca attagacaat
    14761 agtgaatttg aaataacaaa acgacgcggg ctgatcgtat gggtctacag cttaaaacaa
    14821 ttaaagaatc tgaaaaagtt cggcttgttg cattatgtct caagaagaat gaaatatgtc
    14881 gtgctttact taaatgaaga gacattggaa gaaacagaag aacgaatccg taaattacat
    14941 tttgttcgaa acgtagaacg ttcttaccga ccggatatcg aaatgaattt tgccgaaaaa
    15001 ataggaaaca aaacagtatt aaaagacgaa ggtttcgaaa ttgaagagct aaatacagaa
    15061 atccgattag ctgaacatct ttaaaaatct cttatgagca gtttttaaat aaaaaataac
    15121 ttggataacg atcgattttt gatcattgtc caagtatttt tttggtatac tagtaccagt
    15181 accatttcta aggagtgagt agatgcggat aatatcagga aattatggcg ggcgccgttt
    15241 aaaaagttta gcaggggcga acaccagacc aactacagat aaagtaaaag aatccatatt
    15301 caatatgatt ggaccatatt ttgatggaga gacagtattg gatctgtttg caggaagcgg
    15361 tggtttagct atcgaagcag tatctagagg ctgcagtcat gcggtatgcg tagacaaaaa
    15421 ttaccaagcg ctaaaaatca tcaaagaaaa catcgaaatt acgaaagaac cagagaaatt
    15481 taccacaata aaacttgatg cggacaaagc aatcgaacgg ctagcagcgg agaaacaaca
    15541 attcgactac ttgtttcttg atccgccata tgcaaaacaa aaaataatcg atcaaatcca
    15601 taaaatggaa gaactgggac ttttttctaa aaccgcagtt atcgtatgcg aaacggataa
    15661 aacagtcgaa ttgccagaaa ctgtggctgg attccatcaa ataagaaaac agatttatgg
    15721 cataacggcc gtaactatct atagaaaaga gggaaatcaa tgaaacgtgc attattccca
    15781 ggaagttttg atccttttac aaaaggtcac ttagatacgg tggaacgtgc ggctaaatta
    15841 ttcgatgagg tagtaattgg tgtttttatc aatacttcaa agaaaagttt gtttccccca
    15901 gaagaaagaa tgacattgat cacaaaagct gtttctcatc ttcctaatgt aaaagtcatg
    15961 catcaagaaa atcaattgac ggtagaaaca gcaaaagaga ttggtgcaga tgcgttgatc
    16021 agaggcatac gaagtatcaa agactttgaa tatgaacgag agattgctca gatgaatcat
    16081 catctatatg atgaattgga aactgtcttc ttattagcaa aaccagaata tagtcacgtg
    16141 agttccagta ttttaaaaga agtactgcac tttggcggag atgtgagcag ctacttaccg
    16201 ccagtgatca acgaagcttt agctggaaag cgtgaatcaa atgaaagcta aacaatggtt
    16261 gaaaacagta ctgattatca tatttggaat gttgatcatt ggatttgtag tagttccctt
    16321 gccgtattat gtagaatcac cgggagccac gatcgatctg aaagaactaa ttacggtcaa
    16381 tgataaggaa gataaaggtt ctgggtcttt ctctcttacc tctgttggta tcaggcaagc
    16441 tacaggtttc ttagctttaa aagctaaaat gacccctttt gaagaattga tcagtgaaga
    16501 agaattgact ggtggggcga caaatgaaga atataatcaa atgcaacagt attatatgga
    16561 ttcttcccaa aatgcagcaa tcgaacaagc gttaaaactt gcagacaagc cttataaaat
    16621 ggaattcaaa ggcgtatatg tcatgagtgt agagaaaaca tcgaatttct ttggcaagct
    16681 ttcagttgga gatacagtaa caaaagtaga cgggaaaagc tttcaaagta cagaagcatt
    16741 tatggattat gtaaaaagcc aaaaagttgg acaaacaatc gaaatcagtt atttgcataa
    16801 tggtgaagaa aaaacagctt ctggtgatct gatcgagtta ccaacagata aaaaggcagg
    16861 aataggtatc acgcttactg accataccga gatcgaatca gatacagatg ttcgtattaa
    16921 ttctggctct atcggtgggc catcagcggg attgatgttc actttagaaa tctatgaaca
    16981 agtcactggt aaaaacttgc gacatggtaa gcaaattgca ggaactggaa cgatcaacag
    17041 tgaaggagaa gttggccgaa tcggaggaat cgataaaaaa gtggcaagtg cggataaagc
    17101 tggcgtagag attttctttg caccagatga tacgatttca gacgatgtta agaaagaata
    17161 tcctgaaata aaaaccaact acgaagaagc aaaagctgcc gctaaaaaat tgaattcctc
    17221 aatgaaaatc gtaccagtaa aaacagtaca agaagcgctt gactatctag agaaaatgaa
    17281 atgaaactaa ataattaaag aggggttgtg acacgagttc tgtcacaacc cctctttttc
    17341 gtcaaaaatc ttcacgataa aaataaaaac aaaagaaaaa attgacgtaa ttagatgttg
    17401 aagggacgat tttgccagag ctcactgttt cttcaatttt aaatgaaagg agtaatgaat
    17461 gatttttact ggtcattctt tgaaaaaaga acaatgatag aaaaaatcag caagaaaata
    17521 ctagcttcac ccaaattgat catttggatg tctatgatag ttcttatctt ttgtatcgca
    17581 agtggttttt tattatcaaa tttgctattt tatagagaaa cagaagagac tgctgcaaaa
    17641 gaaatacttt cttcgtttag ttccagtgga gaagatccat cagctgaagg aaacgatgta
    17701 gaagaggatg ctcgtccaaa ggtcatgtac accgatatca aaggaagtgt gaaggagcct
    17761 ggtatctatt ccttttcttc agaagaacgg gtctatgatg tactaaagcg tgcaggtgga
    17821 ttattagaag aagcggatag cgatcgaatc aacttttctg cgaagataga agatcagcaa
    17881 gtcctatata tccctgctgt aggtgaagag ctgccagaac atcttgatca gttggcatcc
    17941 tcggaaggaa aacagcctac agcagattcc gagcctccca aaataaatat caatacagcc
    18001 actccatcag aacttcagca gattcctggg atcggatcag tcaaagcgca agaaatcatt
    18061 cgatttagag aagaaaacgg atccttccag aaagtggaag acctgcagga gatttctggt
    18121 atcggtgaaa agacagttga aaaactaaaa aacttcgtta caattaaata gaatgattca
    18181 aaatcgactg gcacgagcaa taaatggacg ctaatggata ctaagaataa tccaattcta
    18241 agagcttggc aggacgagga ggtagtattt ttggatagga aatagcttga ttgttcgcaa
    18301 agaaacatta aaaaaaccaa tgctgaatgt ctatctccaa aataaaatat ctgggattca
    18361 tattatgaat acggctgtca gcggcaataa cagtcaagct ctacgagaac gctttgccaa
    18421 agatgtactt agctatacag cagataaagt atttattctc attgggacga atgatctagc
    18481 agagcataaa caactttcta aagaaacgta ccaaaaaatc tgcagtggat gatagttcag
    18541 ctgaaaaaag actatagttc tgatcatgct ttctttatca cgccaccagc agtcgatgag
    18601 acaaaacaaa aatatcggaa taacaagttg atcaaagaat atatctctgt tctcacagaa
    18661 gtagcaaaac gccatgattg tgaagtatcg atttttatac ggaatttctt gaacaaatgg
    18721 attcgaagaa tttttataat gggctaatgg atgacggact tcatttcggc agtgaagggt
    18781 aggatctttt gtcagacagg atgattcgtt atatttaaag aatattttgt ttaagcaatg
    18841 agtacttttt aatcaacttg aaaaaacgga ggaatataaa acatgttaga aaaatcttca
    18901 aaagcatgcg atacgaataa agaaaaagtt actgataagt cacataatga acgcattcct
    18961 tgggaccagt actttatggc tcaggcagta ctgttatctt tacgtagtac gtgtacacgt
    19021 ttagaagtag gagcgacact cgttcgagaa aaaaggatca ttgctggcgg ctataatgga
    19081 gcagtcagtg gagatgtcca ctgcatagat gaagggtgtt acgtcgtgga tggacactgc
    19141 ttgcgaacga tccatgcaga gatgaatgca ctcctacagt gtgcaaaact tggtatcccg
    19201 acggaaggtt cagaaatcta tgtcacacat tttccatgtt tggcctgtac aaaggctttg
    19261 ttgcaagctg gtgtgaaagg gatcaattat ctccatgact atcgaaatga tccttatgcc
    19321 caagcgttga tcgaacaatt aggtgtatct gtacaccaag ttgctttaga ctcacagcat
    19381 ttttcaaaac ttcaaaatga attaacagga aaacatgtct agcgttttag aaaggcatgt
    19441 tcgaaatcgg ttgatttttc cggcaattac gtcgatgatt atctgtttgt tgtattacac
    19501 tgacaattgg ggttatcgtt tgcttcttgc cggaattttt ttatggtttt cgctaaaaga
    19561 aggacgtttt atcgcgtact tgtgtatttt gtgtagcata acggtatcca tttcttatag
    19621 gctacaagag tattcatttt tagaggaaaa aacaacatct gcgcaaatta ctgtatttac
    19681 agatacgatt cgtgcaaatg gtaatttttt gacaatggaa ggaaggttat cagagaataa
    19741 acaaaagctc cagctttctt atcaagtaaa cacagaagca gaattgaaaa catggcttaa
    19801 cttccgagga aagggaatag aattgactgc tgaaggcgag ttcatctctc cagcaaaaaa
    19861 gcggaatctt tataattttg accaagaagc ttacttcagg aaccatagaa tttctggacg
    19921 gttccaaatt caaaagattg aacagatcac catgcaaaat cactggcgaa actggcttca
    19981 gagaaaaaga ggatctttta tctcctttgt ccaatctgaa tttccgcaaa aaacaagtgt
    20041 atatattaat tctttattat ggggctataa agatgcagga ttcagagaac cagaagaaat
    20101 ttttagggag agtggtttgc tgcatctgtt cagtctatcg ggtctacaca tccaatttta
    20161 tttagggtgg ctgtattatt tatttcgacg tatcggatgg ccactacacg tcagtattat
    20221 tccgttgagt ctactaacta tctgttatgt ttctattgca ggaagttcta tttctgtttt
    20281 gcgtgctagt ttgctgtttt tattgcgatt gctagtcaaa ttgctgaata agcattattc
    20341 caccatggac ttatttgcag ttgtcttttg gcttctttta ctatttgatc ctcgatttct
    20401 ttttcatgcg ggtggacagc ttagtttttt catgagtttt ttgtttatcc tgatttcttt
    20461 tgatggagat tggttgaaaa aaacggcctc tcacgtcctg tttactatga tcacgatgcc
    20521 gttgattgta tggcattttt atgagtggcc tattttaggt agtcttttta cattactgat
    20581 tgcaccggta ttcaagtttc tttttcttcc tggtgtgtta tttctcacac tcttcaatcg
    20641 ctggctgccg gaaactttac ttttgctgat agaagaggga ttaatcttat ttgagcgaat
    20701 aatcgatttt tcttcagggt cgtctctcat tataggacgt ttgccccttg tgctatctgg
    20761 gatgtatatt ttcggattac tgatttgtat ggataggttg aaagaacaac ctgtgaaaca
    20821 cttgttttat gttggtcttt tttcttttat ttttttgact ttgccatttt tacgcttttc
    20881 ttcaacgatc gcttttatcg atgtcggtca aggagacagt atctttttgc aaagtccttt
    20941 tcgaaaagaa acgattttga tcgatactgg aggtcagtta cactttgaaa gagaaaagtg
    21001 ggcgcaaggt atgatacgtc ctcctgctga aagaaatatc gttccatatc taaaaggtca
    21061 ggggataagt gtaatcgata aactcatctt gacacacgac gatacggatc atgtgggaga
    21121 attacccacg ttatctcagc attttactat caaaaaaatt tatatcggtt ggggcgctgg
    21181 acgaaatgag ccattacaaa ggcatttatc tgaagctcaa aaaaatggta ctgagatcat
    21241 tgaaatcaaa catggggaca ggatcagcgg ttattatgat ctttatgtat tgactccttt
    21301 tgaaaaggga gagggaagaa atgaagattc gattggtcta tggatggaat ataacaatag
    21361 gcgcttttta tttttaggag atttgaatca agcaatggaa aaacaattgc ttttgattta
    21421 tcctaatctg aaagccgatg tagtcaaatt aggtcatcac ggtagccgta cgtcaagtaa
    21481 cacagatttt ctttcccata ttgaagcaaa acatgggatt atatcctgcg gcgtaaataa
    21541 tcgttatggt cacccgcaca gcgaagtatt ggagacacta gaagaaaatc agataaaaac
    21601 atggcgaacg gatcaacaag gaatgatcac ctatcgctgg catccagttt ttcatcctca
    21661 tgggctagtg gaaacaatga tagactagta attctcatct gctcatggta ggataatttt
    21721 tgaaagtggt gagtaatttg agtatacaag cagagttaca aaaaatacgt aaagaaaaac
    21781 ttgcatcttg ttacttgatt ttaggaacag aaaagtttct ccaagatcaa gttcggacag
    21841 aaattgtcaa aaaaatccaa ttagagggcg aagatgactt aaatttttta agttttgaca
    21901 tggaaaatgc aagtttagat gaggtagtag ctgaagcaga gacgttgccg tttttcggag
    21961 atcaacggtt agtattcgta gagaatcctt attttttaac aggtgaaaaa gtaaacaatg
    22021 gaatcgaaca aaatactgat ttattgacgg cttatttgaa agaaccatta gaatcgactg
    22081 tattagtatt ctttgcgcca tatgaaaagt tggatgaacg gaaaaaagta acgaaacaat
    22141 tgaaaaaaac agcaattatc atcaatgtcc aacagttgaa tgaaaaagaa gtacgtcaat
    22201 atctgatgaa taccttagag aattcctcaa tcaaaatgga acgaagtgca attgatttat
    22261 tcttgaggtt gaccgactta gatttatcca aaatgatgag agagttacag aaattggtcc
    22321 tttatggaca aaatcaacag caaatcacgg taagagaagt agaagaatta gttcctaaaa
    22381 cgttagaaca taatattttt gacatgaccc aatatatttt aaaagggaag acggaacaag
    22441 ctttgcgttt atacgaagat ttagttatgc aaggggaaga aacaatcaaa attaatgcga
    22501 ttttgctttc tcaattacgt ttatttttac agacaaaatt tcttgtaaag attggttatc
    22561 agcaggcaaa tattgctgaa acattgaaaa tccatccata tcgtgtaaaa cttgccatgc
    22621 aggaagtaaa aaaattcgat gaacatttgc tggtacagtt gtttgatcaa ttagttgaga
    22681 tggattatca aatcaaaacg ggacaaatcg aaaaagaatt aagttttcaa ctattcgttt
    22741 tgcgcacagg gcagttgtta agaaatcgat aagaagaaag acgagggcaa agcatgcggg
    22801 aaggtcgttt gtttttgtcc tttttttgac aaataaaaaa atcggccgaa gccgattctt
    22861 ttatttagcg atttttttgc ttaaacgaga tttgtcgcgg ttagctttgt ttttgtggat
    22921 tagacctttt gattcagcca tatcaatagc tttaacagct tctttatata acgcatctac
    22981 gttgtcagca cctgaagcta cagcatcttc aaattttttg attgcagtac gcatagcgct
    23041 tgtttgagat gaatttttca cattagcgtt ttcgcttgta cgaacacgtt tgattgcaga
    23101 ttcaatgttt ggcattagtt tcacctccga taacttaagt tcttacgcgt gttaccgcat
    23161 aattcaacat tcattattat acctaaaggc agcgtgcttt gcaatatgaa catgacaagt
    23221 ttttttcctt aacagtcttt tattaggatt ttttttcagt aaaaacggat agtacaagct
    23281 gaaaaaaatc aaaaagacgc tattgtggga ggcaatagcg tcaaaccaaa ctagattttt
    23341 atttatctat ttaaacaaag tttgaatggg aatgtttggt actaagttat tatataacaa
    23401 agagaaccgt ttgcaaagtt ttttttgaaa agggatacga ttccctgttg ttctttgttt
    23461 ttctattaag aaactgcagt tattttataa ccagtatcct ttgatcagta aaggctattc
    23521 tggtgtaccg attattttaa tgtattcatc gatatttttt atcaacattt tttttatgac
    23581 caactggttc cgacggttcc atttgccact tacttccagc acatatttat ttaggggcaa
    23641 acgtaagata tattttgcca aatagcgatc tttaagcagg ccattgatca cacggtcctc
    23701 aagctgcaag ttgatcccag tcagcatcat tggctgttct tgtaaaacgt acgtatcaat
    23761 taaaagacct tcaaaagttt ccacaagcgt cttcctttct tttcaaagta acctgaaaac
    23821 aggcactaat tcttccattt tgatattcgt tgaagtggat gcgatcattc tttcttttgt
    23881 ccactgtaat ctagtataac aaaaaagagc agaagagcca aaaagaaaaa agagcatcga
    23941 attctatagg aagtctcatt ggcacggcct aaattattac aaaacattga ttaaaaaaag
    24001 cccctgcatc gcaggagcat cccctatatc gttttaatag gtcaatcaaa aagcagtatc
    24061 aaacacattt tgtatcaaat caggaaataa tagataagct aagcccagaa tcaaaagtac
    24121 cgctagaaga aaccagccga ttgttcctat gaaatacttt aatttttcga acattgtatc
    24181 acctcgttgt cagtatatca cggatcagta aattgggggt gttaagaatc tcctaaaata
    24241 gacaaaattt gttctatttt cctaaagcaa atacttgtaa gatacagtaa gtgataccag
    24301 aagcaatgac cggtccggcg gcgattcctc ttaagaagac aacacctaaa atcgtgccga
    24361 aaacaagtgc aacagtgatt tgtggatcag aagcaatcaa tcctacaccc ttggaagata
    24421 agacagccac tagcccaccg caaaaaacag ctacccagcc aataggggat ttaaaggctt
    24481 ctaatagatc acgtagaccg atttctcctg ttgcgatagg gattagaata gccgctgtga
    24541 tgatcgtcac cccaatattg attcctttgc cttgaagcca agggaaaaag gaacgggagg
    24601 ctggtatgag cttcataatc aaaacaaaag ctgccgctat catcagactt tgatttttag
    24661 ctaaaattgc tacaagtaaa ataattccta aaaaaagcca actttccatg aaatatattc
    24721 ctcactttct tcgaaacaat cataacatgt ttttagaaag ttacaaaagt aggaattcat
    24781 ccttcaagac ctattcataa gctgtttttt tcataaaata aacaaaatca taaaaaaaca
    24841 ctttgcttat ttgacagaag acagccgcgc tttgtatagt ggacaagact tacatttttt
    24901 tgaagaatca gaaagatcag gtaaaggtct tagtaaacgc aagccattca gaataaccaa
    24961 tattgtgctt ccttcgtgtc ctacaactcc aagaggtaga tttaaaaatt gcaggaagtt
    25021 agaagcaatc aacaagagaa taacaagaat cgaaaaaata atattttgtt tgatgatccg
    25081 ccgcattttt aaagatagcc gatgactgta gacaaattta tttaagtcgc ttttcattaa
    25141 gacgatgtct gcgatatcca tagcggcatc tgtcccttgc cccatagcga cacctaaaga
    25201 agcggctgct aaagcaggcg catcattgac tccatcacca accatcgcat ttacttggta
    25261 aagttcattc tcttttttga ctagttgtgt tttttcttcc ggagtacagc ttgcatagta
    25321 atgttcaatc cctacttgtt cagcaatggc ctttgctgtg ccagaatggt cgccggttat
    25381 catacttgta tggatgtttt gggatttaaa gtaggaaatt acttgttggg tatttgcttt
    25441 tggaatatct agcagaccta aaacagcaat taactgctga tctttagaaa gataaatcac
    25501 ggttttgcct tgattttcca aacgttcgat catttccgca atctctttgc tgattatcat
    25561 cgtctcagga tcatatgcgt gcttgccaac tttccattgc gcacctaaat aggtagtttg
    25621 taatccaaaa ccagtaattt cttctattgg taagtattgg atttcttcag gtattgtgca
    25681 atcgaaatat tgcaaaatcg catgagcaag tggatgcgtc gttttttgtt ccatagcaac
    25741 aagaagttgt tgtgccaaat ccttttcatc aaagaaaaag ctgtcagtta caactggttt
    25801 tcctttagtg agcgttcctg ttttatcaaa ggcaatcgct tttaattcag aaagcttttc
    25861 taaatggata ccgcctttga ttaaaatccc attttttgca gaatttgaaa gagcagctag
    25921 agaagctggt gtagcagatg cgacaagcgc acaaggagaa gccacaacta ataataccat
    25981 tcctcgataa aagctttcat cccaactcca acctaggaaa aagtggggga gaaggatcat
    26041 tagaggaaca gcaagcaaaa tcaattttac ataattattt tcgattcgtt cgataaatga
    26101 agctgtttga gttggcgtat tctgcgcttc ttctactaat tggataatct tagcaaagac
    26161 agtttcggaa ctcgtttttg ttactcgaat cgtgatggga tttccttgat tcagcgttcc
    26221 tgcatacatc tcatcgccta aatatttttc tacaggcaca gattcaccgc ttaatgcaga
    26281 ctcatcgatc gttgcctgtg tgctttctaa aaagccatct atcggaacag ctgctccttt
    26341 agcgacaaat accaagttat ccacctttag ttgagaaaca tctacttctt gcatttgtcc
    26401 attttctttt aataattggg ctttttgtgg ttgaagattc atcaaagcag taatttcttt
    26461 ttgactttta ttagttgtgt attcctctaa tgcaccgctt aaacaaaaga tgaatgtcaa
    26521 cattgctcct tcaaagtaat taccgatcaa acaggcacca atagcagcta aagccatcaa
    26581 taaatccaca ttcagtgtcc gatcattcca caattctttc aagccttccg atgtttgttt
    26641 atatccaccg atgataatgg cactgatata aaaaagtggg gtgatggtaa gagagaatcg
    26701 gtcgaagata aaaccgaaga tcataaataa aaggcagccg attgttgctg caaaagcttt
    26761 tttctgatta gtcatcgtta ttagacctcc ttgtcaaaac aaggatagca taataaaaat
    26821 gtaattgata ataataacag ctaaatgaga acgataatgg ttatcatttg gtgattaagc
    26881 ttgatgttgt ttttattatt tataatgatt attattaaag aaaataaaaa accagaacat
    26941 ttctcaaata agagaagtgt tctggttttt tctacattgc aataaaccaa taaccaatca
    27001 aaatgatacc aaggataata cgataccagc caaaagcggt aaagtcgttt ttcttcaaat
    27061 agcttagtaa gaatttgata gctaagacgg aaaccacaaa agatacgaat gttcctacaa
    27121 gaagaacaac agtttcttcc attttgaatg aattcccttc agctaaatat ttgacgatct
    27181 tcaagccgct tgcgccaaac atgacaggaa tccctaagaa aaatgaaaac tctgtagcga
    27241 caaaacgcga agcaccaatc aagatagatc ctaggatcgt tgcgccagaa cgggatgttc
    27301 cgggaacaag tgccaagact tggaataacc caacaagaag agctgcttgg tacgtaaagt
    27361 cattcagaga agtccaacga ggtgttttgt ttttgttgcg tttttcgaca ataataaagg
    27421 caataccgta aacgatcaaa acaatggcaa ctggtaagaa cttgtggaaa tttgcttcta
    27481 accaatcatc caaaggcaaa ccaatgatga tggctggtat gcaagcaacg atcactttaa
    27541 accaaagtgt ccacgtatct tttttttcta ccgttgattt acttggagaa aatgggttta
    27601 acttgtgaaa ataaagaacg acgacagcca agatcgcccc taattggata acgacattga
    27661 acatcgacat aaattgtgaa ctggcatcca gtttgatgaa ttcatctgct aggatcaaat
    27721 gaccagtact actgatgggc agccattccg ttattccttc aataataccg agaatcactg
    27781 ctttgataat atttgcaaaa aacataaatt ccttccttcc caattaagat acatcttata
    27841 gtataccgtt attttttgag aaacccaatc tgttttttaa tattaagaaa aacttgagaa
    27901 ataaagcaga taagtacacg aagagaaaaa agaagacaaa gccctcgttc agactttgcc
    27961 ttttatgatt ctctggtaaa aattaaaata tccccattat agaaacgagt cgcgactatg
    28021 tgttttcccg ttatcgatat aagacgtatc attgatgccg tggatagaaa atcgaccgtt
    28081 tgcataagtg acctttgtta cgctgccatt taaaagccca gcacgaacag gctgtttagg
    28141 atcgatcaga ttaagcaaaa aggcgatcgt caaaccatga gatacgacta aaacatttcc
    28201 accaccattt ttttcgcagt gatgggcgat atcttcaaat cctgtccata cacggtctcg
    28261 caatgtttca taagtttccg cccacccagc ggtatctaag ttgtatacag cattagcaat
    28321 ttgttcaaaa gaaacttctg tggtaaacat ttcatcatat gttttaaaat tcaatacacg
    28381 agggatcaca ccccacattt ccatgtcata tccaccttca agagacccga aacaccattc
    28441 tctaatcctt ttttcaataa aataaggaat ttcttttccc ttagaatgtt ctcctagaat
    28501 aatacgggca gtttcgattg cacgaccact gtcactagaa taagcaaatg aaaagtcgac
    28561 attttttaac ccgcgaccta aataatgaat gccttcttgt ccttgctttg tgaggggtgt
    28621 atcacaccag ccttgtactc gttcaattgt attgaacatc gtttttccgt gtcgtacgat
    28681 ataaagtgta gtttctgtca ttttattctc ctcctaagtc gttttaacga ttgaaaaaag
    28741 gatttgtcct tttttcctgt tcaatcgtcg ttgcatcgcc atgtcctgga tagactgcaa
    28801 attcatcagg taatacaaac agctgggtcc gaatactatg aagtaattgt tctaaattcc
    28861 ctgtatacag atcggttcta ccgatacttc ctctaaacag ggcgtcgcca gaaacgacaa
    28921 aatcatcaaa aataaagctg acgcttccta tagaatgccc tggggtaggg acgacgcgaa
    28981 aagacatgtt tcctaaagta taagttttca tttcaaattc atattctgca ggcgagacaa
    29041 taacatcagc aatatcatca tgtctaccga gtccagaaag attcaaaatc ggatcaccta
    29101 gccatttttg ctctagtggg ctgacataaa ctgggatttg ataatacgcg cgtaaaggtt
    29161 ccactgcacc gatatgatcg tagtgagtat gggtaagcaa aatcgctacc ggctgttggt
    29221 ttgttttttt gatttcttgt ttgattcgtt ccgcatcttc tccagggtca atgatcaaaa
    29281 gtgcttcttc gttgtagacc aaataacagt tttcttcaat cattcctgtt tttaatcttt
    29341 cgatatgcat gtgcattcct ccttgatagc caaaacttct ttcatccatt agtataacgc
    29401 attcattgct aggaaacaaa aacaggtact tatttctctt tacttatctg aattatcaga
    29461 ctatatgaaa agtatttgga tattctaaag agaaggaaag taagttttca tagcatttta
    29521 atatgtcctt gttacattag attcatccaa aaatccaaat tctatatcct atatccctgc
    29581 ctaatcagca gggtgttttt ttatgtttgg aaagagagct ctttttgcta aactaggttt
    29641 tttcaggtaa tcttagagag aaagaggtgg tggaaatgat caaacagtca aaaatatctg
    29701 tgcggcactt agtagaattt attttgcgac gtggaagtat cgacaatcgt aaaaaaagta
    29761 accatactgc attagaagga gcgagaatcc accgcaagct ccaaaaagat gcgggagaaa
    29821 actatgaaaa agaagtcttt ttaaaaacaa cagttcaatt agatgaatat tacctgatcg
    29881 ttgaaggccg tgcagacggt attttcaaaa gagacggtat ttattatata gatgaaatca
    29941 aaacctccga accacgattt gaagatttag aaccagaaca agtggaactt ttctttcatc
    30001 aagcaagggt gtatgcctat atctatagtc aagaacatga tttacaagaa ataaatctgc
    30061 aattgactta cttccagacg gcagaagaag tgatcacgag aaaggttgaa catcagacaa
    30121 gagagcagtt agaaactttt tttaaaaagc tgactgaaga ttataaagaa tggcttgttt
    30181 ttcaagaaaa ctggcgcact gtacgcaatg cctctttgat ggctttgaaa ttcccccatg
    30241 atgagtatag gaaaggtcag cgtgaactag cggtagctgc ttataaaacg atccgtacga
    30301 aacagaaatt gtttgtagaa gcacctaccg gaacaggaaa aacaatctct acgttattcc
    30361 ccgctttgaa agctgtaggg gaagaagagg gagaaaagat cttttatcta acggctaaaa
    30421 caataactag acaagtagct gaagatgcta tgacagcatt aaaagataca ggagctgaag
    30481 taaaaagtgt cactttgacg gcgaaagaca agatctgctt tttagatgag acgacctgta
    30541 atccagatca gtgcccatat gccaatggtt attacaatcg gattaatgaa ggtttatggg
    30601 atcttttgaa tcatgagaat cagatcactc gtgaagtcat cgaaacttat gcaagaaagc
    30661 atacactttg tcctttcgaa ctatcattag atgtcagtat ctggtgtgac gtaatcattg
    30721 gggattacaa ttatttattt gatccaactg tttatttaag acgttttttt gaagatgaaa
    30781 aaaacgagga ctatctgttt ttgattgacg aagcacataa tttggtgaat cgttcccgag
    30841 agatgtattc ggcagaacta tcttatgaaa aaacgaaacg aagtaaggaa gctataccaa
    30901 aggaattcaa aaaacttcat cgccgtttca acaaactatt aaaagaattt gattcgatcc
    30961 gagaaatagc aaaagaagat cattgggatt atcatcatca aaaagcaccg gctgaatctt
    31021 tagtaaaggc aggttatcaa ttaagcgaaa aaattaaaga atggctggca gaatttcctg
    31081 agcacccatc acaagaacaa ttgcttcctt attattttga tttgcttcat tttttgaagg
    31141 tcagtgaatt ttatgatgat cactacgaaa caacagtcga aaaaacgtat cgcgatctga
    31201 tagtaaaaga gttttgtatt gatccaagct tgtttcttga gcagtcgttg gataaaggaa
    31261 aaagcagtat tcttttttct gctagttttt caccactctc ttattatcag gaaacactgg
    31321 ggggacaaaa aagtctggct tacaaactgc cgagtccttt cccagaagga aaccagcaag
    31381 ttttcatcgc aaattaccta gagacgactt atcgaaaaag ggaacacagt ctggcaaaat
    31441 tagtagagac gattcatgct tttgctgatg gaaaggtcgg gaattatttc gttttctttc
    31501 cttcttatca atatttagat cttgcggtgg aagcattcag acagcaatat cccgacagca
    31561 atgtgctaat ccaagaaacg gacatgaatg aggaagaacg cgaacgattt ttaacaaaat
    31621 ttcaaacaaa tcccgctgaa acattgattg gattttgcgt acttggcggg attttttcag
    31681 aaggaatcga tttgagaggg gaccgattga tcggcagtat gatcgtaggt gtaggtttgc
    31741 cgcagatgaa tcatgagcag gaactgatca aatcatatta tgatgagaag gaacaccttg
    31801 gatttgctta cgcttatcag cttcctggaa tgaacaaagt cttgcaagct ggtggtcgag
    31861 tgattcgtgg catggcggat caaggaatcg ttgtattggc agatcaacga ttttctagtt
    31921 tttcttatcg acgtttattc ccacaacatt ggcaaacagc aagagaagta cgaagtgttg
    31981 gggaattgga agaaggaata cagcgttttt ggttatcaaa agaagcagta aatcaaaaaa
    32041 acgaggaatg acagacgtca gcctcgtttt ttgattaacg atagttagtg aattgcaact
    32101 cgattggtaa gtcaagttta cgtaataaag caatcacagc ttgtagatca tcacggttct
    32161 tacctgtcac tctaatctga tcttcttgga tttgtgcttt tactttcagt ttttcatttt
    32221 tgatagctgt agtgattttc ttcgcgtttt cacgatcgat tccgttgacc aaatcggcag
    32281 tttgtcgagc tttgccacct agagcgtgtt ctgattcgga aaaatggata tttttgattg
    32341 gcacatttct tttgatcagc ttaccaaata atacatcttt gatctgttcg attttgaaat
    32401 catcatcgct caatacaact agattgctgt tctctaattt gatttctact gtagaatcct
    32461 tgaagtcaaa gcgattggac aattcttttg ttgtttgctg aatcgcattt tttacttctt
    32521 ctgtattgac ttctgataca atgtcgaagc tagcatcttt tgccatagga accctcctga
    32581 tatacatata aatatttacc atacatctat cttatcatag gattagggca gattcttacc
    32641 tgtttgctaa aattcttctc atttgagttc gaagtatttt attttaatag ataaactgtt
    32701 attcttacat ttggaatacc aaagtagggg agaggtgctt gtgagtaaaa ttatgactgc
    32761 gtatcataaa aatgaaagat taaagaaaat agtggttatt ttgataaccg gattgaccgc
    32821 tgcggtcgct ttaaattatt ttttgattcc agccaatgtc ttttctgcag ggatgaatgg
    32881 gatcgctcaa atcatttcct cgcttttata tgaatggttt catatcaaga tagacacagg
    32941 aacatttatt tttcttttga atatccctgt gtttgtatta gggtttctca aagtaggaaa
    33001 acgagcaacg atccttagtt ttgtgaatgt tgtatgtgta tcggtgatga cgatgctgct
    33061 tccagtcgga cagatcactg ataatatttt gatgaatgcc ctagtaggcg gtgttttatt
    33121 aggtcttggc gtaggattat ctttaaaaat gggctttaca actggtggga tggatatcat
    33181 ctctcttgta ctttcacaaa caactggaaa aacagtcgga aactacatgc tgattctaaa
    33241 tggtatcatt gtagtcatcg caggcttctt ctttaactgg gaaagtgcct tgtatacgat
    33301 tatttccatt tatgcaatga ctcaagtaat tgatgccatc cacacgagtc atcaaaaagt
    33361 aacagcaatg atcgttaccg tcaagccaga agaggtaaca gaagaactag caaaacggat
    33421 ggtacgtgga atgacattac tgccgtctgt cggtggctat tcaaaaaaac aagggaacat
    33481 gatcatgatg gtcatcaccc gctacgaaat gtatgatctt gatcagatcg ttcatgaaat
    33541 cgacgacgat gcatttatca atattttacc aactcattca gtatttggcc ggtttgcaaa
    33601 tgaaaacgaa caaaaaatgt atcgaacaac tggcgtattg ccagaaataa aaaatcgttc
    33661 acaaaaaaaa tgaggaatca ggtttatttt agaataaatt taatctatta catctaataa
    33721 gaacattttt ataaatttaa acgagaatct tacttctaaa ttctcattta caagcagcta
    33781 aaaaaagagt atgataaata aaaatcgtgt taaggagtgt cgtacgatga gcgaagcaaa
    33841 ccaagaacgg tctggacaag aaatagaact aatcaaagaa cgtattcttg ctgccttgga
    33901 aatggtgatt gatcctgaat taggaatcga catcgtcaat ttaggcttga tttatgatat
    33961 agaattcaat cctgaaaatg gagaaacagt cattaagatg actctaacaa ctatgggctg
    34021 tccattagca gatattttaa cagaatctat ccatgacgca ttaaaagaag tgccagaagt
    34081 aacgaaagca gaagtcaaac tagtctggta tccagcatgg acgacagata agatgagccg
    34141 ttatgcacgg attgcgttgg ggatacgata gaaatcttgg ctgactagct atgagaagca
    34201 aataggaaaa acaaaaaagc cttttatcct ttctagaaga aaagagataa aaggtttctt
    34261 tgtttataaa attttgtctg ctatattata agttaattaa ataaatgtgt tctattgata
    34321 aaggacattc aaatcccttt ccttttggaa tccttctttt ttacatcagt ttgattttcg
    34381 tatagtattc tttctcatct tcacgataaa tcgcagcccc atttttttgt tgtgctttaa
    34441 gactgccaag atttttttta tttacagaca aaagtgcctc atcaatgccg attttttttg
    34501 cctcttgtag tactaatgct aatccttgtg tggcatatcc ttttttacga taattagggg
    34561 agatgccgta accaatatgg ccagcgccgt ttcttaatgt ttcgttcagt ttatggcgca
    34621 aattaaaaat tcctacatac tcatgttcat cactttctaa aataaagacg gttgcgggaa
    34681 cgtaactctc aggaagatcg attcctaaag acgttttttc gcattttttt acataatcta
    34741 aaaattcagc aaatgtatag ccataagcat tgttgataaa tccactctca tttggtccaa
    34801 agctagtttg tagctgataa gctttcttta aatctgcttc ccatagtttg atcagtttca
    34861 tcgtattcac ctctttaagt aaccttattc atttgttttc tactatagca ctattgttgt
    34921 aaatagccat gcctattttt atttattgtg tcatatcgta ttaagaatca tcgaacaata
    34981 caaatatgaa ttacaataaa aaaattattt gaagttcgtt cattcgttac ttatgatctc
    35041 tagaagaaag aaaacgtgaa ttacttcagc gtctagaatt ccagaaggat agtaatcaac
    35101 agaagaaata atcaatatcc aaatgaaaga agatagctga atcgagaaaa agaggaaaaa
    35161 agaaatgaag aaaaaaataa aatagttact aatcccagag tcgttcgttt aacattgaaa
    35221 tcgatcatat ggataaaaca gattttttca ccaaccctca atagatgatg gagttgctcg
    35281 gatgaattat ttgtgtagat ggcataaggg agggcgtaca gaaatgaaaa agaacaaaaa
    35341 tatgttcttg acaaaaaaca catgtaatcc taaactaaaa gatgtgatgc ggctatggcg
    35401 gaattggcag acgcgctagc ttcaggcgct agtgagggtg acctcgtgga agttcgagtc
    35461 ttcttagccg cataatttgt ttttttataa agagaaggta cacctggagc gatgagaaag
    35521 aaaataacat attatctaaa agaatatagg aaaaatttta aacaacggaa ccagattcaa
    35581 ataaatcaaa aaaaatttgt gtgaaggtgg gacagtttaa aacctaatag attgattcaa
    35641 atagtagatt tatagattct gatacaagaa atactggcaa acgatagtta aggaataaag
    35701 actaaactta aactcaaaaa gaggtcatgc gaaagaataa atcgcatgac ctcttactat
    35761 attttgaatg tgtttaaacg ttcaaataat gaatcttagt accaaccgtt tgcttcccag
    35821 aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc tgctactctt
    35881 tcttggtttg cagcagagta gtcaccattt aagtaagaag aatctaattg gtaacggccg
    35941 atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc gatccattct
    36001 tttgcagagc ttgtacttgc tggagtagca gcagtttctg ttacagcagg agcaggttgt
    36061 gtttcagcaa caggcgctgc ttgtgtttcc gtttgaaccg tttcttgtac aacaggttgt
    36121 tcagcttgaa caggttcagc ttggactggt gcttcttgaa cggctgcttg aggttcagca
    36181 gctgcttgtg cagagccttc tgttgggatt gttaattgtt gtcctgagta
//
LOCUS       DACECR010000018        34819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.018, whole genome shotgun sequence.
ACCESSION   DACECR010000018 DACECR010000000
VERSION     DACECR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.018"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(233..1081)
                     /locus_tag="IWO17_000832"
     CDS             complement(233..1081)
                     /locus_tag="IWO17_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0289728.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IWO17_000833"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IWO17_000833"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ0289729.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="IWO17_000834"
     CDS             complement(3582..3761)
                     /locus_tag="IWO17_000834"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ0289730.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IWO17_000835"
     CDS             complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IWO17_000835"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0289731.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /locus_tag="IWO17_000836"
     CDS             5909..8188
                     /locus_tag="IWO17_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0289732.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="IWO17_000837"
     CDS             complement(8281..8928)
                     /locus_tag="IWO17_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289733.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="IWO17_000838"
     CDS             complement(9107..9826)
                     /locus_tag="IWO17_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0289734.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="IWO17_000839"
     CDS             complement(9957..11108)
                     /locus_tag="IWO17_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ0289735.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="IWO17_000840"
     CDS             complement(11325..12065)
                     /locus_tag="IWO17_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0289736.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IWO17_000841"
     CDS             complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IWO17_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ0289737.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12449..13042)
                     /locus_tag="IWO17_000842"
     CDS             complement(12449..13042)
                     /locus_tag="IWO17_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0289738.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13673)
                     /locus_tag="IWO17_000843"
     CDS             complement(13026..13673)
                     /locus_tag="IWO17_000843"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ0289739.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IWO17_000844"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IWO17_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ0289740.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IWO17_000845"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IWO17_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ0289741.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="IWO17_000846"
     CDS             complement(15119..15646)
                     /locus_tag="IWO17_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ0289742.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15826..16611)
                     /locus_tag="IWO17_000847"
     CDS             complement(15826..16611)
                     /locus_tag="IWO17_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ0289743.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16604..17473)
                     /locus_tag="IWO17_000848"
     CDS             complement(16604..17473)
                     /locus_tag="IWO17_000848"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ0289744.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17474..18853)
                     /locus_tag="IWO17_000849"
     CDS             complement(17474..18853)
                     /locus_tag="IWO17_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ0289745.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IWO17_000850"
     CDS             complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IWO17_000850"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0289746.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IWO17_000851"
     CDS             complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IWO17_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ0289747.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IWO17_000852"
     CDS             complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IWO17_000852"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ0289748.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IWO17_000853"
     CDS             complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IWO17_000853"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ0289749.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IWO17_000854"
     CDS             complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IWO17_000854"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0289750.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22811..23035)
                     /locus_tag="IWO17_000855"
     CDS             complement(22811..23035)
                     /locus_tag="IWO17_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0289751.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23088..24050)
                     /locus_tag="IWO17_000856"
     CDS             complement(23088..24050)
                     /locus_tag="IWO17_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ0289752.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24060..24500)
                     /locus_tag="IWO17_000857"
     CDS             complement(24060..24500)
                     /locus_tag="IWO17_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0289753.1"
                     /translation="MESDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24806..25771
                     /locus_tag="IWO17_000858"
     CDS             24806..25771
                     /locus_tag="IWO17_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0289754.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25828..26058)
                     /locus_tag="IWO17_000859"
     CDS             complement(25828..26058)
                     /locus_tag="IWO17_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ0289755.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26144..27031)
                     /locus_tag="IWO17_000860"
     CDS             complement(26144..27031)
                     /locus_tag="IWO17_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ0289756.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27120..28343)
                     /locus_tag="IWO17_000861"
     CDS             complement(27120..28343)
                     /locus_tag="IWO17_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0289757.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28360..29439)
                     /locus_tag="IWO17_000862"
     CDS             complement(28360..29439)
                     /locus_tag="IWO17_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0289758.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IWO17_000863"
     CDS             complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IWO17_000863"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ0289759.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31297..31563)
                     /locus_tag="IWO17_000864"
     CDS             complement(31297..31563)
                     /locus_tag="IWO17_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ0289760.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32042..34276
                     /locus_tag="IWO17_000865"
     CDS             32042..34276
                     /locus_tag="IWO17_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0289761.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34424..34744
                     /locus_tag="IWO17_000866"
     CDS             34424..34744
                     /locus_tag="IWO17_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0289762.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9854 a         7518 c         6064 g        11383 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtca atgagacatc ttggatctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15841 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15901 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15961 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    16021 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    16081 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    16141 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16201 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16261 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16321 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16381 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16441 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16501 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16561 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16621 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16681 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16741 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16801 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16861 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16921 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16981 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    17041 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    17101 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    17161 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17221 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17281 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17341 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17401 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17461 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17521 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17581 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17641 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17701 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17761 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17821 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17881 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17941 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    18001 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    18061 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    18121 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18181 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18241 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18301 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18361 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18421 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18481 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18541 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18601 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18661 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18721 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18781 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18841 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18901 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18961 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    19021 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    19081 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    19141 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19201 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19261 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19321 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19381 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19441 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19501 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19561 tctttttctt tcattatttc tggagcagtt gttgcttgtt cagatatgac attgactggc
    19621 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19681 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19741 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19801 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19861 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19921 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19981 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    20041 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    20101 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    20161 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20221 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20281 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20341 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20401 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20461 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20521 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20581 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20641 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20701 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20761 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20821 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20881 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20941 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    21001 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    21061 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    21121 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21181 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21241 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21301 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21361 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21421 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21481 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21541 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21601 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21661 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21721 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21781 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21841 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21901 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21961 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    22021 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    22081 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    22141 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22201 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22261 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22321 cattttcccg cttccagctg gggtagcttc tgtcatataa gcgcctcttt ttgctactaa
    22381 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22441 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22501 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22561 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22621 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22681 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22741 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22801 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22861 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22921 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22981 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    23041 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    23101 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    23161 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23221 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23281 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23341 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23401 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23461 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23521 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23581 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23641 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23701 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23761 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23821 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23881 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23941 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    24001 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    24061 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    24121 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24181 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24241 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24301 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24361 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24421 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24481 gtttttaaat ctgattccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24541 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24601 tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa
    24661 tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg
    24721 aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt
    24781 tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct
    24841 gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa
    24901 acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt
    24961 attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga
    25021 tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat
    25081 gaattatttc agtaattata attatgtaca tattaccaat aagggatttg tattaacaga
    25141 tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt
    25201 gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt
    25261 gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aggaactcca
    25321 agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa gacaacaaca
    25381 tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata tcgaattagt
    25441 tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac catcgctcac
    25501 cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg tttgttatgg
    25561 tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct attatgaaaa
    25621 gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca
    25681 aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg aactgttaaa
    25741 caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt aggcatcttc
    25801 aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt ccatcgctac
    25861 gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta attcaaatgt
    25921 atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt caaaacggcg
    25981 aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta tgattgcgac
    26041 taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa
    26101 gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat tttttttctg
    26161 gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca ttctcaaaac
    26221 ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc aatacaagat
    26281 ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa
    26341 aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa gaccagttag
    26401 ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg tttttttgtg
    26461 catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc tgattagcca
    26521 tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta ccaaattctt
    26581 tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg atcgtcagtg
    26641 taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca tctgcgcctt
    26701 tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta gtcaaatcaa
    26761 ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag tagatttctt
    26821 ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct gctaccgctt
    26881 ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt gcttttgtcc
    26941 gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca
    27001 tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc
    27061 ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat gtttgaattt
    27121 tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc ttcttctttt
    27181 cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat catagcttcg
    27241 ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg atctttagga
    27301 acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt
    27361 ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc cacgacacaa
    27421 ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga agtagaagca
    27481 ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc cacttctcct
    27541 gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa ctcttctaca
    27601 taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat gacctgagga
    27661 aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa agaaagaatc
    27721 atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc ctgctgagtg
    27781 atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct
    27841 attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac tacacctgtc
    27901 gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat cacttttcct
    27961 ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat cagaggtatt
    28021 ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt atgggtatga
    28081 ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc tctaacaaca
    28141 actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac atctttttcc
    28201 aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc atgtctttct
    28261 aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg aaagtaggta
    28321 tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga cctgatttac
    28381 tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat ttttaagttc
    28441 ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt gtgtcgtact
    28501 cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt ttgtcttccc
    28561 catatagcag ttcttgctat cttctaacca gccattatac actggaatat ctcgaacaag
    28621 tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct cttcataaga
    28681 agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc cacgaatgta
    28741 accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga tgttccgagg
    28801 aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca tcttcttagc
    28861 aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac aaatgattac
    28921 tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca ttggatcagg
    28981 gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac ggataccata
    29041 gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg ctttttggta
    29101 caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatccga taaaggaatt
    29161 tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt ctcttctggc
    29221 tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga tgtcataatc
    29281 ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt tttgatgatc
    29341 aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat ataataatac
    29401 cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac tgtcactcaa
    29461 acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa aaagaacaaa
    29521 aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt tattttacat
    29581 attcatgaac aagttcaacg acttcttcca tcatatcgca ttctttcaat gcacgatcag
    29641 caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt ttaagaatag
    29701 atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga acagctgttt
    29761 gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt gctgcatcaa
    29821 ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag taagaaacgc
    29881 gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta cctacagaga
    29941 agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga atctcgatca
    30001 tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat ttttgttttt
    30061 cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc atagggaaca
    30121 tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt gtacggaaca
    30181 tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag aatggattca
    30241 tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc attgtacgaa
    30301 caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca tattggtcat
    30361 cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac agaccaactg
    30421 cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg tttgcagcta
    30481 attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt tcccattctg
    30541 ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt tcatctgtag
    30601 gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct tctttgactt
    30661 tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt gccatgatag
    30721 cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta
    30781 attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct tcgttgatca
    30841 ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta gcaacgtcac
    30901 gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg aacataccga
    30961 tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg tctttaatat
    31021 tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg gcatcaaata
    31081 cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc tgcaattctt
    31141 cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta tcttctactg
    31201 ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca acggctacac
    31261 cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc cttctttctt
    31321 cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg cagtaatagt
    31381 aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt tcaagtttac
    31441 tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg tttgtactaa
    31501 tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt ctttcttttc
    31561 catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt cttttttagt
    31621 aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca ttttttttcg
    31681 ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt ccggattctt
    31741 tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc ttgtcgcttt
    31801 ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat atgaaacgaa
    31861 ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga atggtcaata
    31921 gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat tcttttacct
    31981 catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac gtgaatgatt
    32041 catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa
    32101 tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca aaaatcaaca
    32161 aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca gtttggacga
    32221 tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc aaacaccacc
    32281 aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag gcaatacaga
    32341 aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag gtgatatcga
    32401 tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa atcgccgaac
    32461 caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag ttgttgaagg
    32521 tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca aagaagtcat
    32581 tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat ttgaagaacg
    32641 tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat tcatagatga
    32701 agttcacgaa atcgtcggtg ctggttctgc tggtgatgga aatatggatg ctggtaatat
    32761 tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta ccttaaatga
    32821 gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg tacgggtaga
    32881 tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga
    32941 ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt tatccaatcg
    33001 ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg aaacaggttc
    33061 taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga
    33121 agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag cagcttatta
    33181 tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac
    33241 acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag gtattccagt
    33301 gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg acttgaaagc
    33361 tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg
    33421 tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg gaccaactgg
    33481 tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt cacaagattc
    33541 aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta aattgattgg
    33601 ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa aagtacggag
    33661 aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag atgttttgca
    33721 tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa gaacggtcag
    33781 ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag tagaagcaaa
    33841 cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc aattaaacaa
    33901 ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca gtgcattatc
    33961 aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc aaatgctggc
    34021 agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag ttgacttagg
    34081 ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga
    34141 tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag caaaactgga
    34201 taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag aatcagccga
    34261 agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg agaaatgccg
    34321 tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag aaattataat
    34381 aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat tggtaaatgt
    34441 aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg cccatctagt
    34501 caaggtctac acagccggaa ataccactat tgtctacact gaagcccctg aacataatga
    34561 aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa
    34621 ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca aagtaatcga
    34681 attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta cattagccgt
    34741 gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaagtaaag aacacttttg
    34801 tctcacgctc taaacacgt
//
LOCUS       DACECR010000019        34464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.019, whole genome shotgun sequence.
ACCESSION   DACECR010000019 DACECR010000000
VERSION     DACECR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.019"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(68..1960)
                     /locus_tag="IWO17_000867"
     CDS             complement(68..1960)
                     /locus_tag="IWO17_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0289763.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(1957..3684)
                     /locus_tag="IWO17_000868"
     CDS             complement(1957..3684)
                     /locus_tag="IWO17_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0289764.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3700..4350)
                     /locus_tag="IWO17_000869"
     CDS             complement(3700..4350)
                     /locus_tag="IWO17_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0289765.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4554..4850
                     /locus_tag="IWO17_000870"
     CDS             4554..4850
                     /locus_tag="IWO17_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0289766.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5022..5642
                     /locus_tag="IWO17_000871"
     CDS             5022..5642
                     /locus_tag="IWO17_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ0289767.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5734..6900)
                     /locus_tag="IWO17_000872"
     CDS             complement(5734..6900)
                     /locus_tag="IWO17_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0289768.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(6912..7265)
                     /locus_tag="IWO17_000873"
     CDS             complement(6912..7265)
                     /locus_tag="IWO17_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0289769.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7393..8415
                     /locus_tag="IWO17_000874"
     CDS             7393..8415
                     /locus_tag="IWO17_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ0289770.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(8854..9288)
                     /locus_tag="IWO17_000875"
     CDS             complement(8854..9288)
                     /locus_tag="IWO17_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289771.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9398..11257)
                     /locus_tag="IWO17_000876"
     CDS             complement(9398..11257)
                     /locus_tag="IWO17_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0289772.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11375..12535)
                     /locus_tag="IWO17_000877"
     CDS             complement(11375..12535)
                     /locus_tag="IWO17_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ0289773.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12842..14266)
                     /gene="araA"
                     /locus_tag="IWO17_000878"
     CDS             complement(12842..14266)
                     /gene="araA"
                     /locus_tag="IWO17_000878"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ0289774.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14282..14980)
                     /locus_tag="IWO17_000879"
     CDS             complement(14282..14980)
                     /locus_tag="IWO17_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0289775.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(14937..16598)
                     /locus_tag="IWO17_000880"
     CDS             complement(14937..16598)
                     /locus_tag="IWO17_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ0289776.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16816..18216
                     /locus_tag="IWO17_000881"
     CDS             16816..18216
                     /locus_tag="IWO17_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ0289777.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18468..19547)
                     /locus_tag="IWO17_000882"
     CDS             complement(18468..19547)
                     /locus_tag="IWO17_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0289778.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19633..20592)
                     /locus_tag="IWO17_000883"
     CDS             complement(19633..20592)
                     /locus_tag="IWO17_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ0289779.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20604..22127)
                     /locus_tag="IWO17_000884"
     CDS             complement(20604..22127)
                     /locus_tag="IWO17_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0289780.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22137..22700)
                     /locus_tag="IWO17_000885"
     CDS             complement(22137..22700)
                     /locus_tag="IWO17_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0289781.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22706..23569)
                     /locus_tag="IWO17_000886"
     CDS             complement(22706..23569)
                     /locus_tag="IWO17_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0289782.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23583..24479)
                     /locus_tag="IWO17_000887"
     CDS             complement(23583..24479)
                     /locus_tag="IWO17_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0289783.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24654..25925)
                     /locus_tag="IWO17_000888"
     CDS             complement(24654..25925)
                     /locus_tag="IWO17_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0289784.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(25915..27432)
                     /locus_tag="IWO17_000889"
     CDS             complement(25915..27432)
                     /locus_tag="IWO17_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0289785.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27630..28520
                     /locus_tag="IWO17_000890"
     CDS             27630..28520
                     /locus_tag="IWO17_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ0289786.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28593..29417)
                     /gene="nadE"
                     /locus_tag="IWO17_000891"
     CDS             complement(28593..29417)
                     /gene="nadE"
                     /locus_tag="IWO17_000891"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ0289787.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29429..30901)
                     /locus_tag="IWO17_000892"
     CDS             complement(29429..30901)
                     /locus_tag="IWO17_000892"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ0289788.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31538..31900)
                     /locus_tag="IWO17_000893"
     CDS             complement(31538..31900)
                     /locus_tag="IWO17_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0289789.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32123..32875
                     /locus_tag="IWO17_000894"
     CDS             32123..32875
                     /locus_tag="IWO17_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0289790.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33015..33938)
                     /locus_tag="IWO17_000895"
     CDS             complement(33015..33938)
                     /locus_tag="IWO17_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0289791.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(33953..34225)
                     /locus_tag="IWO17_000896"
     CDS             complement(33953..34225)
                     /locus_tag="IWO17_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289792.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10182 a         7177 c         5916 g        11189 t
ORIGIN      
        1 tcggaaaaag ggactgtgac acgacttttg tcacagcccc taaaaaaaat aataaatttg
       61 tactattcta tgaagcttct tcttcagaaa actgactgtt ataaagatct gcatagaatc
      121 cgtctttttc catcaatgta tcgtggtttc ctgtttctac gatcgaacca tgattcatca
      181 caataatgtt gtctgcatcg cgtattgtag acaagcggtg agcaacaacg aaacttgtcc
      241 ggttttccaa taggcggttc atcgcttttt gaatcagtat ttctgttcgt gtatctacac
      301 tagatgttgc ttcatcaagg atcaggacat ctggatttgc taagaatgcc cgcgcaattg
      361 tgattagctg acgctgccct tgcgaaatat tgcttgcatc ctcgtttaat acagtgttat
      421 atccttctgg caatttacga acaaaatcgt ctacgtgagc agctttagca gcctcaatga
      481 tttgttcgtc tgttgcatcg tcgttcccat atttgatatt gtcatagata cttcctgtaa
      541 acagccaagt atcttgcagt accattgaaa actgtgcgcg caattcatca cgtgaaagat
      601 cacgggtatc tacaccatcg taacgaatac tacctccaga aacatcgtag aaccgttcaa
      661 gaaggttgat cagtgtcgtc ttccctgcac cagtcggacc tacaatagcg accatctcac
      721 caggtttgac atccaagtta aaatcagtca ttagcaagtt atctggtgtg tagccgaatt
      781 gaacattttc aaaacgaact ttgtatggtg tatctttttc ttctggaagg ccagattttt
      841 cattggacat ctcttcttca tccaacactt caaatacccg ttcggctgaa gcaactgttg
      901 attggatagt gttcatcaaa cttgcaagtt gagtgattgg ttgagagaac tgtgttgtat
      961 attgcaagaa cgcttgaaca tcccctaatg tcatgttccc atttgcaact tttactccgc
     1021 ctagtacagc gacgaacaca tagcctagat ttttaacgaa attcatcaac ggcatgatga
     1081 ttgccgaaat aaattgagct ttccaaccag cggcatacag attctgattt tctttttcaa
     1141 atacttcctg gtctttttca gtatgattga atgttttaac aatggtatga ccaccatatg
     1201 tttcttctac ttggttattc aataaaccta aacttttttg ctgatctgcg aaaaacactt
     1261 gtgatttcgg cgctacgatc atcacgatga tcaagcttaa agggatcatc aataacgcga
     1321 ctaacgtcaa ttgccagctg attgaaagca tcatcacgat gaccccaatc aatgtgactg
     1381 cactagttac aaattgagtc aaattttgct gcagcgtgct tgcgatgtta tccatatcat
     1441 tgattgctcg agacatgata tccccatttg tatgtgtatc ataataggaa atcggtacac
     1501 gattcatttt ggcttccaaa tcatgacgta attcataaac tgttcgttgg gaaacacgtg
     1561 tcataataaa ttgctgtaaa aagttgaata cagcagaaat caaatacatc acgattactg
     1621 tcgcaatgat ctgcgcgatt ttatcaaagt ctatcggaaa ttttgttact tgttggcctt
     1681 gttgcatctg ttgagcgcca gccattacac ctttgaatat ttctgtggtt gctttcccca
     1741 agacctttgg tgtttggatt tggaagatca cggctgctat tgctagtaca aatacagcga
     1801 tgatcgcaac catacgttta gacatatagc caaacaatcg gcgtaccgtt ccccagaaat
     1861 ttttaggctt ttcacctttt acaccgagat ttctgccagg tccaccatgt cctggaccgc
     1921 ctgctggtcc atgagaggga cgtgtgtttg ttttactcat gctaagtcct cctctcttag
     1981 ttgtgaatga acgatttctt gataagtttc gtttgttgct aatagttctt catgtgttcc
     2041 tttgccaacc agttttcctt catctagcac aaggatcata tctgcatcca ttactgtact
     2101 tactcgttga gcaactaaaa cggtgatact ttctttcata ttcgttttca atgcttggcg
     2161 aagattggca tccgttttga aatccaacgc tgagaaggaa tcatcaaaca catagatatc
     2221 tgctgttttg actaatgccc gtgcaattgc cagacgctgt ctttgtccac cagagaagtt
     2281 tcctcctcct tgttccacat ggctatctaa gccatcttcc atctctgaaa caaaatcttt
     2341 agcttgtgcg atttctaatg ctttccaaat catttcatct gttgcatccg gcttaccata
     2401 ctgcatattg tctcggatcg ttccagagaa cagaactgct ttttgaggaa caaagcccat
     2461 taattctcgc aaattatatt gagtcatgtc actaatgtcc gttccattta tttggatcga
     2521 accagattcg atgtcatata atcgagggat caaattgact aaggtcgttt tacctgaacc
     2581 agtcccccct atgattgcga cgatctcgcc cgacttggct tggaaatcaa tatcttccaa
     2641 tgcaagtttt tctgctccat gatatcgata attgacatga ttgaaggcca atgttgcgtt
     2701 ttttccagca aaagaaactg ttttaggatt ttcagggtca tggataccga ttttttcatc
     2761 caacacttca ttaatacggt cagctgaagc ttgtgcacga ggcaccatga taaaaatagc
     2821 ggaaagcatc ataaagctga tcaagatctg catcgcataa gtcataaatg caatcagatt
     2881 accgacttcc aaagtcatat ctgctatgta gtgtccacca aaccaagtga ttgctacgtt
     2941 tgtaccgctc atgatcaatg tcatcattgg catcatcaat gcgacgatcg tattaacttt
     3001 gattgccgta tttgtataat ctttattcgc taaatcaaaa cgattttctt caaagcgtgt
     3061 cttatcgaat gctcgaatca ctctgacccc tgtcaatcct tctcgaaata ccaggtttaa
     3121 acggtcagtt ttcttttgca tacttttgaa taatggaaca gcaaagtaca tgattccgcc
     3181 gatcaaaata atcattactg ggatgacaac taagaagatt tttgtcaact gatgatcttt
     3241 ttgataagca agtatgctcg cgccaatcaa cgtgattggt gcattgatca tcatacgtaa
     3301 aaacatttgt gtaaccattt ggatttgagt cacatcattg gtcgttcttg tgatcaaaga
     3361 ggccgttcca tatttatcaa acgcatcttt tgtcagactc tctgattttt tatagacatc
     3421 cgtacgcaat tgcttaccca gtttctgtga ttcacgagta gcaaaaaatg tatttgcgat
     3481 agcagctaag atactgatca aagaaaagcc gatcatcaca aaacctgtat gccaaatata
     3541 atcgacatct ccttgcgcta cccctttatc aataatattg gaggtaagtg tcggcaaata
     3601 taaatctgcc agtacttgga tcaccatgaa aactgcagct gcaattgctg aagtcaagga
     3661 gatccgtttt acgattttta gcatctagtt cccttctttc tatttatata ctccgttttt
     3721 cagccaagaa agtttcaaat tgaattcact gattgcttgc tctactgagt aatcttcaga
     3781 tgttgaaaat aagcgatacg aatgcgcaat tgttgtaaat acagtattca tcactaattg
     3841 gatcagtaat tccaattcat gatcattttt tactttcagc ttcgtccgat cgatcaattc
     3901 aagcaactct tttgcatctt gtttgtgttc tttatgtgga tcgtgaggcg ttttttggcg
     3961 actcgtatga ggagcgactt ttcttgaggc acggtaatcc atgttcataa acaagttacg
     4021 ataaaaagaa gcattctcgc cttccaaaat ctcataaatc attttagaaa aataggattc
     4081 gaatcctttg aatagatcac catcggattc tttcattagt tgtaacatat ctttagagct
     4141 gtcacgtctc attgtctcaa agtaataaaa ataaagatcc tctttatctt caaaatactg
     4201 atagaaactg cctctaggga tatccgctaa tttgacgata ttagcaatcg aagcttcttt
     4261 taagggtact ctagaaaact cgattctcgc agcctcgatt agtctttttt gtttttcttt
     4321 aggcaaatgg aaaaatgtct gtgtcggcat atggatcctc ctttctttca agatgacaca
     4381 ttgtcatatg acgctgtgtc attataacac ctcctacctg ttttgcaaga catttgcata
     4441 gtttttttca ttatcatccg atacattaaa aaagtgataa gaggaatcct tccatttgaa
     4501 ttaatgagag ttgcccatta aaatagccac atactaaaca aaggatgaga taaatggccc
     4561 aatcaattag tgtccaagac ttcaataaat tgcctctcga tgctgacagc atcgttttag
     4621 atacaagaaa taccttagat tacacaaaaa accacttaag ccactcgctt tctatccccg
     4681 ctgctatctt gccaagacaa ttaaaaaatc tagatccaaa gaaactttat tatattctaa
     4741 gctatacagg caggcgatca gaagcattag ccgatcaact tactgctaat ggttttcgtg
     4801 ctgtttatat cattggtggg atggaagctc ttcagtcgag tgttgcttga atattgcttg
     4861 ttagattcaa aagaggctgt gacaaaaaat cgtgtcacag cctcttttat accctgtctt
     4921 tttttagtta tttattcttc aaccttatca atatgacttg atagcctttt ctttttctca
     4981 tagttataac aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag
     5041 tcaaaggtca tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt
     5101 taacaagtta taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg
     5161 atgatattcc ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg
     5221 cagaattcac ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga
     5281 ctgatcagtt ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata
     5341 tcccaacaaa attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct
     5401 atacagaaaa cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta
     5461 atggcggtgt atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact
     5521 ttgtaggcat cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc
     5581 gtgcagatga gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt
     5641 aaaaattgag ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg
     5701 taccttggat attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt
     5761 ttcttcataa agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt
     5821 agctgctgca taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga
     5881 agtcagcaga actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa
     5941 tggagagatc aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat
     6001 gatcatgaat ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga
     6061 tcctaagtaa gggatcaggt tcaaaaaacc cgcaataatc cccaacgtga cagcatactc
     6121 taaaccaatg accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag
     6181 ttgtcctctt atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg
     6241 tttttgcatg cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt
     6301 caatagataa aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc
     6361 gccgacaaaa cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc
     6421 tgccgaattc atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag
     6481 cgggtcccgc aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt
     6541 actgatgaag ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc
     6601 gattagtgca acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg
     6661 cttttcaaag taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat
     6721 aggcagacct acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt
     6781 aaataaaaag acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt
     6841 atttaaaaac cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat
     6901 aattcctcct gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc
     6961 ggatgaagat agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat
     7021 gatacgtgag ctactgtctc taaattccca ttggccatcg gaccgacata atcgatcgta
     7081 tatacctgat catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt
     7141 gatacttctt gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa
     7201 atcagcatgg gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga
     7261 atcattgcac tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta
     7321 gccgtgtgga ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag
     7381 gaatcagctc atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa
     7441 ggaatctata cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct
     7501 gttgaagaaa atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt
     7561 actagtgtgg atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat
     7621 gcggtaacag agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta
     7681 ctatacgggg cgaactatca taaaggtgaa gtaaatgtct accatatatt aggagatggc
     7741 gaactgaaag cagcagattc aatattccat caagaagcga caggtcctca taaaaaccaa
     7801 gatcatgcgc atgtccacta tacggatctg actcctgacc aacgattagt cgtatgtgat
     7861 cttggtacag atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt gaatcttgta
     7921 actaccttca ctgcagagcc aggaacaggc cctcgccatc ttgtctttca tccaaatgga
     7981 caattcgctt atttatttgg tgaattagac agcacagtcc gcgtgctttc ttataataaa
     8041 gaaacaggtg cttttgaaga attacagaaa atatcgacct tgccagaaga attcgacggt
     8101 gaaaatggag gggcagctat ccgcatttca aatgatggca agttccttta tgcttctaac
     8161 cgaggacata actcgattgc tgtattcgca gtttttgaag atggttccca cattcaaaac
     8221 aagcaaatca tctcaacaga aggggatttt ccccgcgatt tcgcactaga tccaagcaat
     8281 aattttgtcg tgtgtgccaa tcagaacacc gataatctga cattatatcg ccgtcatcct
     8341 gaaacaggat tactggaatt actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt
     8401 tattttgatc gttagtaaga aaaggagaat gaaatgaatc agcaagacgt attagacaga
     8461 ctacgggaag aactaaatat tcctttcttt gatgggaaaa tcgaagataa agagtatagc
     8521 gaagaggaat atcagaaact caaagccgat ttacagaact actttgaaca atatgtacag
     8581 aatgtagaaa actaatcagc tttccatact atctgaaaag aacatcagga caagactttt
     8641 ccgtctatcc tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt agactcgacg
     8701 gaaaaggagt atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt
     8761 cattatcata aaggattttg atcactctat cataaaggta atattacatt tatattagat
     8821 aactctagtt tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat
     8881 tttttggatg gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt
     8941 gaatagatct tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc
     9001 tgctacatat gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg
     9061 tggacgcaaa gtactgactt tcttaagcat tgcccgatat ttttttgttc cttcttcaaa
     9121 taacaggatg atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat
     9181 tgcgtttctg atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt
     9241 gacgatttga tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct
     9301 actttctcca ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact
     9361 tgaaaaaatt taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat
     9421 ctgctaaact atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt
     9481 aaatctcttg ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt
     9541 acagttctcg ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc
     9601 catttgttgt ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga
     9661 ttgcatcgtg agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg
     9721 tttcatgcaa tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa
     9781 gatgatcccc ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga
     9841 actgcttcag tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg
     9901 tcccacttcc atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg
     9961 gcatatgtgt ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa
    10021 gtgtcattac ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat
    10081 ccgtataggt catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat
    10141 cctctctttt atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg
    10201 cgtttaacgc tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag
    10261 gggtggtgag ttgcgcatta aataacgcca tagaaaaacc agtcaatgct tcaaattcga
    10321 tatttgctgt tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag
    10381 ctacttcata ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactag
    10441 gatctgagaa actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt
    10501 tatttttttc atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg
    10561 aatatccttc aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa
    10621 aaccagtgtt atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat
    10681 atgcttttcg caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa
    10741 ttgccacaag cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc
    10801 gaaacatgta ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa
    10861 acagcatagt gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt
    10921 ctggataaat cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac
    10981 cgatggctac gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa
    11041 aaataagaat aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa
    11101 aagcaaattt caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat
    11161 tactaaaaac aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt
    11221 tctgtttggt cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc
    11281 aataaaagaa tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa
    11341 agaagaatta atgaaatcca aatattctct ttctttatgg cctttctggt ttctttaata
    11401 agcggacctt tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt
    11461 caaaatcagt actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg
    11521 atatatctgc gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat
    11581 tggttttctt ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg
    11641 atggataatt gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa
    11701 ctgccaaacg tccgctgtct gccaaagctt cctgagtcga tccccctaga tgaggcaaca
    11761 taagtatacg gtcattttct aagaactctt cttctggaaa atcagtcaag tatttagcca
    11821 gatgttgttt ttccaatgct tgcataacag catatttatc aacaatttca cttcttccta
    11881 cattgataag tacagcatct tttttcattt tttctatatt cgcctgatcg atcaatcctt
    11941 ttgtatcttc tgtcaacggc agcatgacta cgatgtaatc cgaggtagaa agaactgttt
    12001 ctaaatctgc ttgccgaaca taatccaact gggcatcgcg gatagaataa cccaagacat
    12061 ccatccctaa gctatagcaa cttagcgcta cttttgtgcc tatcgctcca agaccaagaa
    12121 gaccgatggt tttgccttgc aattctcgtc ccacataagc acttcttttg ttttctgcct
    12181 gttctaaaat attaggacct gttagtgttt gtaccatacg acttgcttcg attatcggac
    12241 gggaagataa gagcaaacag cacaaaacaa gctcttttac tgcattagca tttacacctg
    12301 gtgtattcat gacgatcgtc ccgttttcag aagctttttc aacgttgatc gtgttcaccc
    12361 caacaccagc tcttgaaatt gctagcaaat ctggtgtaag ccattcatct ttcactttcg
    12421 tactacgaat aactattcct tcggctggca aatcatctat tattttgaaa tattcccctt
    12481 gtaacttttc tatctcttgt tctggaatag catcaatcaa atgaacgtga tacataaaac
    12541 cccctcctta ctaacaagta tctcgaaatt tctgcaatta gacaactgtt taccatttga
    12601 cttcctagaa gcaaaaagag cagttagagc agagtaggct gagtgataaa aataaggcta
    12661 tgacaagtta ctgccataag ccttattttc gagagcaaaa tattgagggt gagatatctt
    12721 gataaatggt gttccgttag ctgacattga taataagact aaatttcaaa aaagttgaaa
    12781 agcaaatttc gtcaatctct tcttatttct taaaactaag tggctttttc acaacctctt
    12841 tttatctaat aaccactgtt tccaactcaa ctaattttgc ccacgtttca atttgatctg
    12901 ttgttagatt tagcgagaca acggtatgat gaccgccacc tgcctgaatc catgaatgga
    12961 caccttcatg gaaattcggt ttagttttcc ataacacacg tgcaacaggt aaattaggtg
    13021 cttcagtcgt tggttcaaag gcttctactt cattgatcag cagtttataa tgcgtaccga
    13081 aatctgccat cgatacgact accccttctc ctgctttccc atcaaataca agacgcgccg
    13141 gatcttctct atctcccatt gataaaggag aaacaacgat tcttggtttc gtcgcagcaa
    13201 gcgtcggatc tacttccatc atatgggatt ccaagattgc ttcttggcca ctagctagct
    13261 cgtaagtata atcttccatg aatcctgtat ctttccctcg agccatgatt ttcagcaacc
    13321 gatcaatcgc tgcagttttc cagtcgcctt ccccagcaaa accgtatcct tctgccatca
    13381 aacgttgtac agctagtcca ggtagttgtt tcattccata caggtcttca aaattagtcg
    13441 taaaagcgtt atagcctcct tcttctaaga aacggcgaat accgatttcc tgctgagctt
    13501 gtacttttac atgttcctcc catgtctttt cttcataatc accatagtcg aaggtataca
    13561 attccttgta ctcttcaaat aattgatcga cttcttcgtc tgatacgcga tcgattactt
    13621 gtacaagatc tccgatacca aaataatcaa cagtccaacc aaattgaatc tgtgcttcca
    13681 ctttgtctcc ttctgtgaca cccacgtttc tcatgttgtc accaaaacga gccactttta
    13741 ttccaaaact ttcattatag gctactgcaa catccatcca atcagcaatc tctttttgga
    13801 tctcaggtcg tttccagtag ccaacgacga ttttattttg tttatttaat cgagcattga
    13861 tgaacccata ttcgcggtca ccatgagcgg attgatttaa attcatgaaa tccatatcga
    13921 tcgttttcca aggaatgctt tcgttatatt gcgtagctaa atgaagcaat ggtttttgta
    13981 gaagctttgt cccgcggatc cacatttttg ctggtgagaa agtatgcatc caagtgatca
    14041 cacctgctac ttcttctcga taattgacct ctttcatgat ttttgtgatc gtatcagcag
    14101 taactgctaa ttcttgaagg cggattggat aaggcaattg gccattttca ttcagttcat
    14161 cgaccatttc ttgtgcatgt ttctttactt ctcgcagtgc ttcttcccca tataaatgct
    14221 gtgatcctac aacaaaccaa aattcttttt ctccaatatt caacataatt agccactcct
    14281 tttatttgtt ttgtccgtaa tacgcatttt ttccgtgttt tcgtaaataa tgtttatcta
    14341 aaatccgttg aggcagctct tctgaaaatg aattgagctg tctggtaaat aaattcatct
    14401 tgcatacttc atctagtacg actgcgttca ttaccgcact ttgcgcatct ttcccccaag
    14461 taaatggacc gtgtccgtgt aaaagtaccc ctggaattgc taagggatca attttgcgtt
    14521 ctttgaatgt ttctatgata acttttcctg tttcatattc gtacccgctg tcgatttcct
    14581 gttgtgtcaa aaatcgagca caaggtaccg atccataaaa agtatctgca tgcgtcgtcc
    14641 ccatcgcagg aacatccaat cctgcttgtg cccagatcgt cgcccatgtc gagtgagtat
    14701 ggacgattcc tccgatttga ggaaatgaac gatataaaac agcatgagta ggcgtatccg
    14761 aagagggatt gagtttacct tcaatgatat gattgtccaa gtccacaacg accatgtcgt
    14821 ctgctgtcat ctcttcataa ctgactccgc taggtttgat aacgaaatat cgtgtttcag
    14881 gatcaaaagc gctcacattt ccccatgtat atttgacgag tccgtgttta ggcaagtcaa
    14941 gatttgcttg gtatacttct tctttcagtt gttccagcat tttctatcac tcctttttct
    15001 tgcaattgat gaatcgctgt tttttcaatg tcgatcccct cacggtaacg tctcataaat
    15061 atctgatagc ctgctatttc ttctgctgaa ggtgtgatgg tgattccttg gtcttgtcca
    15121 aacacgtctt ctgccaagaa gttttctaaa gacttcccat gtcgatccat catataggct
    15181 gctaataacg cgattcccca cgctccacct tctccggctg tgtccatcac tgtaactggt
    15241 gcctccatag cagatgctag aatccgctgt ccaacttctg gtgttttaaa aatcccccca
    15301 tgccccacta aacgatcaat ttggacattc tcagatttca atatatccat tccgattctc
    15361 attgcaccaa aagcactggc taaatggatc cgcatgaaat tcgctaaatc aaaacggcta
    15421 cctggtgtac gtacaaataa tggccttcct tcatttactc ctgtaatgtt ttcaccggaa
    15481 aaataaccat aagaaagtaa tcccccgcaa tccgtatcgc cttctagcgc tttgttgaat
    15541 aataccgtga ataatttatc tctgcttatc tccataccca aagaattggt aaattcttca
    15601 aaaatactga cccaagcatt gatatccgaa gagcagttat tcgtatgaac catgcctact
    15661 agttctcctg aaggtgtagt gaccatgtcg atttccggat ggacattttc tagttctttt
    15721 tctaatacga tcatagcgaa ggcggaagta ccagccgaaa tgttccctgt ccgtttggct
    15781 acactgttcg tagcgaccat accggttcct gcatcccctt ctggcggaca aaccgggatt
    15841 cctggtctca aatttcccgt agggtcgatc agataagccc ctgattcgct taacttgcca
    15901 gcttcttctc ctgctactag aactttaggc aaaatgtgat ccaagtcaat cgctacgtgc
    15961 gctttttcga ttaactgacc aaaaatattt ttcatttctt gatggtaggt tttcgttgaa
    16021 ggatcgatag gaaacatgcc tgatgcatct cctacgccta gtactttctc accagtaagc
    16081 tgccaatgga cataacctgc aagcgttgtc aaaaaattaa tttctttcac atgttcttct
    16141 ttattcaaaa tagattgata caaatgagcg atactccatc tctgcggaat attgtattga
    16201 aatttttccg ttaatattcg ttctgcttct tcagtgattg tatttcgcca agtacgaaat
    16261 ggtactagta actgttcctt gctatcaaat gctagataac cgtgcatcat tgcactgaac
    16321 ccaatcgacc cgattgtcgt aagattctcg ccatagattt cgaatacttc tgctgccatt
    16381 tttcggtaac tgacgcgcag tcctttccaa atatcttcta atgaataggt ccagatacca
    16441 ttttctagtt tgttttccca ctcataacta ccagcggcaa taggagtaaa agatgaatca
    16501 atcaatacag ctttgatcct agttgatcca aactcaatcc ctaaagatgt acgaccatct
    16561 atgacatcta acatactttg cgcatttact tccttcataa tgatttaact ccctccttct
    16621 cgtcaaacct gatcaaaccg tctttttagt agcgcttaca atgattattg tatacttttg
    16681 tacgtacatg tcaagcggtt atttacaaga atacaataaa aaacacttgt tttgtacgta
    16741 cataagttat aaaatgaaat tgcttacaaa atctgatcaa ccgccttctc gaaattctag
    16801 aaggagagaa tacttatgaa aaaagaaaaa aaagtttcct catcgtttat ttattttttt
    16861 ggcgcatttg gcggcattct atttggatat gatattggtg ttatgaccgg cgccttacct
    16921 tttttacagc atgattgggg tttggcaggt aaagcttctt tgataggatg gatcacgtct
    16981 tcggtcatgt taggtgcgat ccttggcggc tctttatctg gtcttctttc tgataagctt
    17041 ggacgacgaa aaatgattct tctttctgca cttattttta tggctggttc tgtcctttct
    17101 gcatctgccc ctcacaatgg ttcttatttt ctgattgcag caaggattct tctaggttta
    17161 gctgttggcg ctgcatctgc cctagttcct gcttatatgt ctgaaatggc tcctgctaga
    17221 ctaaggggaa gattgtcagg gatcaatcaa gtgatgattg cttctggcat gctcctttcc
    17281 tatgttgcag attatttgct aaaagtgttg cctgaaacga tggcttggcg cgtgatgtta
    17341 gggctcgctg ctgtccccgc gctgatttta tttttcggtg ttttagctct accagagtca
    17401 ccgcgttttc ttatgcaatc tggccgcttg gaagaggcaa aaagagtatt gaactatatt
    17461 cggacaccaa aagaagctga acaggaattc gaacaaatcc agctgaatgt caaacaagaa
    17521 aagacaaccg gaacttcttg gcatactttg tttttagaaa aatatcgttc cttagtcatt
    17581 gccggtattg gtgtagcagt attccagcaa tttcaaggag ctaatgctat tttttactat
    17641 attccgctga tcgtagaaaa agcgactggg catgcagcga gcgatgcatt gatgtggccg
    17701 atcatccaag gaatcatttt agttgctggt tctttgctct ttttggttat tgcagacaag
    17761 ttcaatcggc gaactttatt gaaaataggc ggtagcgtga tgggactttc attcatccta
    17821 ccagctgttt taggaactgt actggatgct catacaaatt cgttgttgat tctcttgttt
    17881 ttatgtattt atgtcgcatt ttattcttgc acatgggctc cactgacatg ggtaatcata
    17941 ggcgaaatat tcccactagc tgtccgcggg cgagcatctg gcctagcctc ttcttttaat
    18001 tggattggtt ctttcctagt tggcttgctt tttcctgtga tgactgcttc tatgtctcaa
    18061 gaaatcgttt tcggcatttt tgggatcatc tgctttttag gggttttatt tattcaagag
    18121 atcgtaccag aaactcgtgg gaaatcttta gaggaaatcg aacaaagtgc atctaaaaaa
    18181 acttatccta aacgtatttc tatccatcat tcttaaagag gttgtgacag aagtggtcag
    18241 cttcaagaaa taagaaggaa ccgcttagtt tccagtcaca aggaacaatt tcaaaaaaca
    18301 gcttctcatg tttgatgaat attgtgactt gtgaccagtt ggtaggagaa caccgtttag
    18361 tggagaggtg gtgaaagaaa cgttcagcct tgagcgatga caaaagctct cacaaacatt
    18421 gcgacgagta accgtaggag caataagagg cggtaaaaac ccgtctctta tttttcgttt
    18481 tctttcactg tttcccgttc aactagtaca ggttcataga gaatatttgg ctgtgcctgt
    18541 cctttttcaa tcgttgtgac gatccaccgt gcggcatctt gtcccaactt ttcttttgga
    18601 tgcgtcaatg ttgtcaattt tttattccct aaatggctta aagaagaatc gtctgttcct
    18661 acgatcgata ggtcatctgg aatatgatag ccattttctg tcaacttatc tagcattaaa
    18721 ctagctattt gatcgttata gcaaatgatg ccatctaaag cttgttcttc tttcaaccgt
    18781 tgtactgttt tttcagcgat tgtttctcgt gtttccgtgg tgaaagtaat gatatcttcc
    18841 gtcaaaaaac gcacattata ctgttcacac gcgctgataa aacctttcat cctgtatttt
    18901 ccttgaagat cgtcgatttt tgtgatgaac aacagacgct gatgtccttt ttttatcaaa
    18961 tattctgctg ccataaaacc gactttcaca tcatctaaac aaatataaga ggcatttaac
    19021 tcttcataag cagcattgat catcaccatc ggaatgcctt gttctctcaa agcaacataa
    19081 agtgctaagt ttgggttata ctgattactt ttcgtaggct caacgatcaa accatctacc
    19141 ccataagcaa tcattttctt caaacactct ttctccaatt cgtgatcgtt attcgtactg
    19201 gctaaaagca atgaataccc agattctcgt aattcatttt cgattcctcg gataatcgac
    19261 ggaaaaatat aatcagagag atatgtggtg atgacaccga tcgtttttgt tctgttcaat
    19321 ggtgtttttt ctttcttata gctgtcatct acatatgtgc ccgacccttt ttctttacgc
    19381 agatagccct catttaccaa cacagcgatt gcttgccgaa tcgtatgtct gctgacgtca
    19441 tatatttctt gcagcttgaa ttctgaggga atggttgtat ttacttcgta ttcctcacga
    19501 aggatctttt gttttatctc ttctgcaata tcttgatatt ttgccatttg ccttgcccct
    19561 ttttatttgt acgtacatat atcataacga gtctgcaagg tttattcaag caaaaatcaa
    19621 ggtttcttgt gctcataagg catacggaac aggttcacca aattctggaa atccttgctc
    19681 attccacgta aagcattgcg cgttcgcgtg ccgattagga ttgtctaacg gatccccttt
    19741 ttggttcttc tctggtcgag catgatagat caaaatatca aagcacccgt cttctgaaac
    19801 cgtaaacgag ttgtgaccag gaccaaattg cttatttttt tcagaagaaa cgaatactgg
    19861 ctccgataat ttatgccatg attcttttaa taaaagattc ttgtgctcat cagcccacag
    19921 caggcccatg gcatagtttt catctgttgc actaccagaa tacgtaatga atattttccc
    19981 attgcgagta aggactgctg gaccttcatt tactgaaaaa ccccgttttt cccattcata
    20041 ctcaggtata gaaaggagga tctgcttccc ctttaacgtc caaggattat ccatttcgga
    20101 tagataaaga ttagaattcc cagtaatttc aggatcttgt tgggcccaca cataatatag
    20161 ctttccttga gaagagaaag ttgttccgtc taaactgaaa gtttcccttt tcgtttttat
    20221 ttgtcccttt tctatccaat ctgtattcat tggatctata tgctgatttt caagtacgaa
    20281 catgcggtga tgatgagatt catcacgtat agttttatga ttgcttgcag caaagtaaat
    20341 ataccatttt ccattgatat aatggacttc aggtgcccag atcaattcac tcatcggtcc
    20401 ttcttcatga gcctgccata caatcaggct tgcagcatgt tctaattcat tcagtgactt
    20461 tgcccttctt aattcaatca cttggtagcc tggaaccgat cctgtaaaat aataataacc
    20521 atctgtatgt ttatataccc aaggatctgc ccgttgccga acgataggat tattataaag
    20581 tgtcttcatc ataacaaatt tcctcacatt ttctttattc taattacatt ccaagagtat
    20641 ttttctatcc ctacatttaa ttcgtcatgt ttttctattg aaaaatagtt tatttcaggt
    20701 tttatttttt ctggttcttc acgtgtattt ttgtccaaca gatcgctact ggttaatgtg
    20761 tagtgctctc cttgatctag caaattgaaa tcatgttcaa tcgttatctt ttgttgttca
    20821 tttgtgcgat tgatcaagaa aatagtcagc tgattgccgt tatctacgat cacactgtcc
    20881 acataaggaa cattttttat cttttcacaa tcataaacag ggcaaatgat ttgttgtttc
    20941 aatacttgac ctttagcata tttcgataag tgttgcatca cataataagt tggattcctc
    21001 catgcttgtc ctttagcatc tgtcagtatt aaaggtattg tgtttactaa caatgattga
    21061 caactaatct ttacgcgatc tgcgtgtcgt aaaatagcca gccctaatga accaaataat
    21121 aatgtatctt ccatagtaaa gtaacaccaa tcgatgggac taccgacttc aaaatcccta
    21181 tgtctttttt ccggttgggc atgtacattc cattcatcaa acgctagata cattgttttc
    21241 tcacttctca aatatccttt tacataatca caagtagcaa cgatttcttc aatttgacga
    21301 tcgaatcgat aagcttttgc aaggtatgtc gctgtatcat cacgctcatt tttaggaggt
    21361 ttagtcaagt cttcttcatc atacttatca atatagttat gcaaagctaa ataatctaca
    21421 ttctcataag cttcttctaa tgcgatacga tcccattcag ggtacgatgg taaacgtggc
    21481 gtagagcttc caactaagat tgtttcaatt gaatcatcca ctaacttcat gacttttgag
    21541 gcttcatttg ctaacctacc atattcataa gcactcttag cccccacttg ccatggaccg
    21601 tctagctcat tccctaaaca ccataattta acgccaaaag gctttttttg cccatttttt
    21661 attcttaaat cactccagaa ggttcctttt ggaaaattac aatattctac taaatttttt
    21721 gcttcatcaa ttccttttgt tcctaaattc accgtgaata tcggtttcgc ttgtacatga
    21781 ctgatccatt taaaaaattc gtggagtcca aaagaatttg gttctatacc tcgccatgct
    21841 aaatcaattt tttttggtcg ttgttctttc ctacctacag tatccaccca atcataacca
    21901 gatgtaaagt ttcctccagg atagcgaatg acacctaatt ctaattcctt tacataagag
    21961 aggacatctt gtctaaaccc gtcaccatca gctgtttcat ggcccggttc ataaatccct
    22021 gtatagacga cacttcccat atgctcaaca aatgagccaa acagccgaga actgatctct
    22081 cctaccgtaa aatcctctct gatttttact ttcacttctt cgtccaacag ctttccctac
    22141 ctttcatttc ctatagtcaa gataatagat acaagtttcc atacgccacc agcaaagata
    22201 aaaataaaag gagggaaaag aaatatgagc agtacaaatc ctaataacaa tacgcaacaa
    22261 agaatatttc tcaaaaaatc aaaaaatact ttccatgcac tttgttcaaa aatttgtaaa
    22321 aatgtgatat ctttgttttc agaaatcaca tatatccagg ataaaagaaa catggaataa
    22381 ataagtaaaa ctacatatcc actaattgaa agtacgattg tctgttcttc taataattga
    22441 ttaacaagaa aataaataat gatcgttaac agactaagga acaagttctt accaaaatgt
    22501 tgctttattc cttgaaagaa aaaataaatg atatttcctg atccctcacc tttccattgc
    22561 gccattacat aaaaaacagc acatgtagca ggcacgatag taataacagg caaacacacg
    22621 attacccaga gaatacctaa taagatgtaa tcagaaacca caatcaactg tccataaaat
    22681 ttagtctcaa ctattttcat cttcctcact ctttcattcc agacatagct gttccttgaa
    22741 caaaatattt ctgtgcaaaa atataaacca ctagtaatgg cagcattgaa ataaccgttc
    22801 cagccattac aggtccataa aaagaaatgt tctgccctgt aaataaagct aaccctgctg
    22861 gtaaagttct catttttgaa ctacttgtca atatcaatgg ataaagcaag tcattccaac
    22921 tattcatcag cgtgaaaatc cctaatgata aaagagtagg tttgctcaat ggcaacatga
    22981 ttttcaaaaa aatattaaac tcacttaatc catcaatgcg tgctgcctct tccaaagaat
    23041 ctggtaaagt aataaaaaag gaacgcatca tgaagatacc aaatgcagtt gtcatacgag
    23101 gaatgatcaa acctgcataa gtatcaagca tgccaagcaa attgacttca ataaatagag
    23161 gaatcatgaa gacttggaat gggatcatca ttgtcagcaa aacgaaatag aacaaaactg
    23221 attttccttt gaaacgcata cgagcaaatg cataccccgc aagagaatca aagaatacag
    23281 aagtgatcac tgtccctcca gcaaaaatca ctgtattttt gatgtaatct aataaaggga
    23341 tcgttgtcca aaccttaata tagttatcta gggtatagct atctgcaaat attctaggcg
    23401 gaaatgagat aatgtctttt tcgtgtttaa aagaagaaat aatcagccac aaaaaaggaa
    23461 acgcaataac caacaatata actagcatac taatcgttcg aatgattttt ttaccactaa
    23521 ctgcttttcg actattcaca gtaactgtaa tcgagctttc tggtgccata caggtttccc
    23581 tcctatttca tttgtttttc tttccgtgac atgtacacat tagtggagat agctatagtt
    23641 gcaacaatga taaacaaaac cgaagacagt gctgatgcat agcccaattc ataaggtgct
    23701 gtaaatcctc gactgtaaat atattgaaca gctgtttctg tcttaaattg tgggccacca
    23761 gcggttgcaa catatacttg gtcaaacact tgcatcgaac caatcagatt cgttaaaata
    23821 caaaaaccta aggagggaac gatctgaggt agcgtaatgt taaaaaactg ctgtcgattt
    23881 gttgcaccat ccatttgagc agattcatac aaagagggag agattccctg aatagcagtc
    23941 aagagaatcg tcatagtaac accaaaattc ttccaaacag tcatgatggc taccgtcggc
    24001 atagcaaggg ttggatcgcg aaaaaattga gggatcggca agccgacttg tgaaaacaag
    24061 taaggaatcg cccctacatt cggatcaagc aacatagaaa aaataatcgc aattgacgtc
    24121 aatgaacaaa taactggaat gtaaaatgtc gaacgacaaa aacgattgaa tctggtattt
    24181 ttagataatg ctactgctgc taataaacca acgataattt gtgttggcgt ttcgagtacg
    24241 gtgaaatata aagaatgaat caacgaatta actgctcgtt catcactaaa tatacgtgtg
    24301 aaattttcaa aacttacaaa atttcttgat gtaaagaaaa tattcaaatc tgtaaaacta
    24361 atagcaaatg ttcctaaaag cggtatgaca gtaaataata ataaaactaa aaaagatgga
    24421 gttaaaaaca aatacgcagc acgatcagga cgattccaat atttggtgaa tccgttcata
    24481 tagtccacct catctttcat tttgataaaa tatacaatct tggtttaaac gaatctgaat
    24541 aagatatccg aacaatcgct gtgatttttg caataacacc tatctgcaaa tactaggtcg
    24601 atcattcaga tatctgtatc ttgccaacag tctatcctgt ataaagttct gtactatttt
    24661 tgcagtaatt gattgatggc atcatctgca tcgttcatga gtgtctcagg atcttctcct
    24721 gctaataatt tttcaatcag tggattgatg atatcgttat tgatcgcaga gatttgggta
    24781 aaacctggta aatatggctc tgcatagtcc atttgtttcg ataattcaga tacaatagga
    24841 tcatttttta cttcttcatc ttttgctact gaatccaagt aggcaggaaa accattttcg
    24901 atactccatt ttttaccaat ttcagttgta ttccaatatt tcaaaaactc atagacagct
    24961 tcttttttat tattatctgt acttgccgga atggcatatc ctacgccatc taaaatagct
    25021 gtttttttgt ctgaatcact cccttgtggt aaggtagtca cattataatc aatctcgttt
    25081 gctttcaatc cgttatttaa ccatggccca ttgatttcaa tggctagttg accagcattc
    25141 attaaattgt ccatttcggc gccagaaatc gattctggac cagtcttgtc tacatggatc
    25201 attttttgga tcgcttttaa cgttttaagg ttctcaggag acgcaaattc agacttagta
    25261 taatcatcgt tcactaattt accgccattt gctaacatcc agttatataa cggtgcagtt
    25321 ccatccttat tgaagacaaa acctgataca tttttactac tatcagttaa ttttggtgcc
    25381 atttctgtca attcttccca agtagtaggt ggttgttcag tatctaatcc agcttgttta
    25441 aacaaatcat tattccaata catatacatg ccttgtacct gcatggggac aaaatactgt
    25501 ttaccttcta cttcacctaa ttcaatagcc gtttctttaa aatcagattt atctactcca
    25561 tcatattccc agaaatcgtc taatggttgt accgctccgt tttctacata ctgtgcaaaa
    25621 tctggataat ttaatgcaat aaagtcaggt gcttttttag aagaaatcgc tgtaggaagt
    25681 ttttcattca aattggccca agtcatgata tccatttcaa ttttgatgtt tttatcattt
    25741 gttttattaa attcattaac gatttcttgc aatatttcac catcagatgc agtaaaccca
    25801 ttccaaaaag tcaaagtcac ttctttatca gaatcagatt ctgctgaact tccgccaccg
    25861 caagctccta aaatacctgt tgcagctacc aacaaagagc caattaacca ttttttacct
    25921 ttcatttttt gttcctcctt catctgataa acgacatctg accacattcc aagatagcgg
    25981 tttcaataaa caattgattt tcccatcttc agcttttaca tttgttgaat gacttggttt
    26041 tacttgttca cttttaggaa gattagttgc ttttgtatca aatccactca tttcagaaaa
    26101 atcaatcact ccctctaatg ataattcaga caagtgaaca gttagattca tttcttcttc
    26161 tccacggtta actgcaaata tgacaagttc tttgtcattt attacggcta tactttctaa
    26221 atacggcact tcttcaaaat cttccgtcgc atagtttgct gaacgaattt gtgggcttaa
    26281 cacctctcct tgtccataca cagcagcttg cataaatgga taatagatcg tctgtctcca
    26341 agcttctccg tttgtttctg tcctgattgg tgcaataacg tttaccaatt gagcaagaca
    26401 agcaatcttt actctatctg catgtttcaa taaagtgatt aatagacagc ctaatagtaa
    26461 tgcatcttca aaattatagt tatcttctag taaaggagga gcaatctgcc aaggcgctgc
    26521 ttgctcatct tgttccgctg aatgatacca aatattccat tcatcaaagg acaggttgat
    26581 ctgcttctta ctatgtttct tcgccttgac tgtatcgcac atcgctgtca cttcagaaat
    26641 gaaccgatcc atgtctaagg aacgtgcaag ataatgagaa agatcatttt tttggttgcc
    26701 ataatattga tgcagagaca aataatctat ttgatcgtac gcttgatcaa gaactgttaa
    26761 ctcccaatca ccaaatgtag gcatttgccg attcgaactt ccgcataaaa ccacctcaat
    26821 tgaatcatct acgagtttca tcgccttagc cgtttcctct gctaaccgtc catattcttc
    26881 cgctgtttta tgtccaatct gccaaggacc gtccatttca ttccctaaac accatgtctt
    26941 aattccaaaa gggctctctt gaccattcga acgccttaaa tcactccaat aagttccttt
    27001 aggaaaatta caatattcga ctaaattacg agctgcatct actccacgag tacccagatt
    27061 gacagccata ttgatttcgg cccccacatg ctgacaccag ttataaaatt catgaatccc
    27121 aacttcattc gtttcaattg tccgccaagc taaatccaac cttctaggtc tatttgctat
    27181 gggcccaata ctgtcttccc aacgaaatcc tgacacaaaa tttccaccag gatatctaat
    27241 catgggaatg ttcaattctt ttaccaagtg aatgacgtct tggcgaaatc cacttttatt
    27301 agctgatgga tgatctggct gataaatccc ttcatatact gctcgtccca tatgttcaat
    27361 aaatgaactg tacaaccgcg gatcaacttt gcctacacgc ttgacactac taatcaacat
    27421 tgtggcttcc ataactgacc tcctttaatt tatacatgaa taatacacct cgttgaaagc
    27481 gttgtcaata atataaatgt taaacttact tattttttat cgattttaat taaccaaaat
    27541 attaataatg attaaaacaa ataatttaat ataactttta ttaacaaata tgttaatata
    27601 taagaaaaac cttttaagag gtgatatata tgcaactaca aataaacgaa gaatccttac
    27661 cggtatatga agcattagct agtaaaacaa ggatcaagat catccagcta ctttccaaaa
    27721 aaaagatgaa tgtcaaagat ttggcaaaag aacttggagt gagcagtgct atcacgacga
    27781 tgcacgtgaa aaagctagag gaagcaaaca tcatcaaaac agaaaaagta ggacaacaaa
    27841 aaatttcgag ccttcgtgtc gataaaatcg atattagttt tccagaaaaa atattcaatg
    27901 cttttgatac gaaagaaaca tcgataccta ttggtcacta tacaaattat gcaattgaac
    27961 caacatgcgg tttagctacg atccatgatt ttatcgggaa agtggatgaa ccacgctatt
    28021 ttatggatcc tcgaaggatg gatgcccgca ttctctggtt taccagcgga tttgtcgagt
    28081 atcaggcacc taatttttta aatacagagg atacattgga gatgttggaa gtttctgtgg
    28141 aaatttcttc tgaatttcct ttttcaaatg acaattggcc ttcagatatc actttcagcc
    28201 taaatggtgt ggagctagga acatggacga gccctggaga ttttgctgat atccgcggta
    28261 aatatacgcc tgattggtat ccagataatc ttaatcaata cggcttgctg aagacgatcc
    28321 gaattaccaa acacttgacg aatatgaatg gagaaccctt gtcaaacatc acgatcaacg
    28381 acatccctaa agagcaagac acttggcatt tgcgaatcga agtgaaagac gatgccaaac
    28441 atgtgggggg ctgtacttta tttggtaaag gattcggtaa ttacgatcag gatatcaaat
    28501 taaaagttta ctacagctga tatttttctt ttctatttta taaaacaaaa acagccaatc
    28561 cctatgaagg gacggctgtt tttgctttat tgttatttcc agaaatcatc caaaacggaa
    28621 attggcaaat gtcgtttatg ttgtgtcttg ttccaccatg cttcgatttt ttcttgtgct
    28681 tgagcgggaa cttcttttcc ttctagataa tcatcaattt cattatatgt gacacctagt
    28741 gcgacttcat ctgcaatcat tggtttccca tcttcaagat ctgcagtcgg aatttttgtg
    28801 tataattttt ccggtgcatt caatgcttga agcaattgtt tcccttgtcg tttgtccaaa
    28861 cggaataatg gcaaaatgtc ggctccacca tcaccaaatt tcgtgaagaa tccagtaatg
    28921 ttttctgcag catgatctgt ccctaatacg gctcctgctc tttctccagc tacggcatat
    28981 tgcgtaatca tacgttgacg tgctttgata tttcctttgt taaaatcgct gattcccaca
    29041 cctgcttcgc ttaatacttg tacttgtgca tcaacagctg gtttgatatt tactcgcaga
    29101 ctaacatcgg gctggataaa gtcaagagca gcttttgcat cttcttcatc tgcttgttca
    29161 ccataaggaa gacgaacagc gataaattga tagttttcat cttttgtttc ttcacgcatt
    29221 tcttccatcg ttagttgcgc caaacggcct gctaaagtag aatcttggcc gccgctgata
    29281 cctagtacaa aagttttaag aaacggatat tttttcaaat atgctttcat aaagtcaata
    29341 cttttacgta tttcttcttt tggatcaatt gttggtttga cacctaattc attgataatt
    29401 tctgtctgca ggctcattta ttctcctcct atgcttctgt atgaagttct gctacgcttt
    29461 ttttcattcg ttccatgata gccattttat ggttccagca ttcagtagac agatcgactg
    29521 ggtatttttg cggattcaag tctcgtttat actcttccca tagtgaatct agattttctt
    29581 ttgtatattc tttgatttcg tttagtgcag gtaattcgta aactcttttt ccttcgataa
    29641 agatatcttg taaaactggg cgagctgtaa agtctcgaac agttttattg ataaacgtat
    29701 gcactggatg gaacatgaag atagcttctt cttctcgagg gtcttcatcc cataatgtca
    29761 cgtagtctcc ttcagatttc ccatcaaaat ttcgagtgat gcgccacact tgttttttcc
    29821 ctggagtagt tactttttct gcattgcttg ataacttgat cgtatccatc atttttcctt
    29881 catcatcttc gatcgatact agcttgaata ctgcaccaag ggctggctga tcataggctg
    29941 tgatcaattt tgtcccaaca ccccatacat caattttagc tttttgcatc tttaaattca
    30001 aaatcgttgc ttcatccaag tcattcgaag catagatttt tgcttccgta aatccggctg
    30061 catctaattg ttcccgaaca cgcttagata tataggccat atctccactg tcgatgcgta
    30121 cgccttggaa gttgattttg tcacctaatt ctttcgccac gcgaatcgca cttggcacac
    30181 ctgatttcaa cgtatcataa gtgtcgacta agaacacgca gtctttatgg gttttagcgt
    30241 aggccataaa tgcttcataa tcatttccat aagattggac taacgaatga gcatgggtcc
    30301 cgctagctgg tatcccaaat atcttcccag cacgtacatt gcttgttgca tctgctccgc
    30361 cgatatatgc cgctctagtt ccccagactg ctgcatccag ttcttgtgcc cgtctagttc
    30421 caaattccaa tagtggatct tctccgatga cagatttgat acgagctgct tttgtcgcga
    30481 tcaacgtttg gaaattgacc atattcaaca gtgctgtttc tactaattgg cagtgcgcta
    30541 aaggcccttc cacttggatc aatggttcat ttgcaaaaac tagttctcct tcacgagcag
    30601 aacgaactgt tgctttaaat tcaaaatttt gtaaatactc caaaaaacct tcaggataga
    30661 cttctaatcc acgcaagtaa gcaatatcgg aatcattgaa cgtcagattc tctaaataat
    30721 tgactaaccg ttccaatcct gcaaaaaccg cgtatccgtg gttaaaaggc atttctcgaa
    30781 agtaacattc aaaaacagca tgtaaatcag ctcgacctaa ttcccagtat gtctgcatca
    30841 tattgatttg atatagatcc gtatgcagcg tcaaactgtc gtctgggtaa gtattattca
    30901 tcttgtatta ctcctcttgg caagttttca tttcctaccc attataacaa aaaaaattaa
    30961 taggaaaaga aatcttccat tttttctgaa aaagcacatg ttttacggat atttcttgct
    31021 aaaaatgaac tccatctgct aaaactatcc tttcttattg gtatttaacc taaacgagaa
    31081 agaatactta ttttattcaa tgtatccgag acacgaagaa attagcgttt tctatttttc
    31141 ttacttgata aaataagtag attttcctac tacaatcgta gccgttaaga taccgatgat
    31201 tgaacaaata taaccaaatg tctctacaac ataatcaatg gcaaaagttt ttgaagcaat
    31261 aatacgtctt tccttttttg cttcttatcc ccaaagaaac ggatataact tattatgtat
    31321 tgaatttttt tggaaaggcg aattatgaca agatatacct tgcgttgaaa aaactcgcgg
    31381 aaaaaatatc acacgttttt aaaaagatac aaagtaagaa agcagctgtc aatagaatgg
    31441 aaacaaaaat ggcgagacat caatcagtct cgccttgttt tttattgtat acattagata
    31501 tggagcaggt taccataaag atgattatta gtcgttatta aatagaatcc ttcgtatttt
    31561 tttgttctat cactacaata tctttatttg gacgtttgaa ttgtattccg gcacgttcta
    31621 gctcctgttt cactcctaca tggactttga catcttctcg tgtcattttt aaaacgtgat
    31681 gaatcgcata gtcgatttct tgccttgtga tttttttctt cgtattgatc atcacgactt
    31741 gctttttatc atatgtgtct accaatagca gttgtgttcg atctaacgaa aataatttgt
    31801 aataattaat agccgtattc ccaaaaaaga attccactac tttaggataa aatgtcttaa
    31861 tatttaaatt actattcaca ggtgtttcga ttagcttcat ctcattcctc ttttctatat
    31921 agtcgattta gatccaatct taagttatgt taccatcttt cctagcatta aggaatcggt
    31981 tacaattgaa cctttaaaga caaaaggatt taccttttat cacttaatag tataacataa
    32041 tttcatgaga aaaaaataca attttttctt gaccatctcg ttacgtaaag gattatgctt
    32101 acatttgtca ggaggaaaaa taatggagta cacaataaag aaaatgtcag agatatcagg
    32161 agtcagtccg agaaccttgc gcttttatga tgagattgga ctgctaaaac cagctaggat
    32221 caattcttca ggttatcgga tttatggcaa aaaagaagtc gatcgtctgc aacacatctt
    32281 attttatcga acaatggcat tcaagttgga tgatatccaa gaggtcttag acaatccatc
    32341 ttttgaccac cagaaagctt taatcaagca tcagcaaatg ttgctggaaa aacgggcaca
    32401 aatcgatacc ctcttgacaa ctgtccaaca gacacttgat atgtacgaag gaggaagaaa
    32461 aatgagtgac aaagaaaaat ttgaagggtt taaacaacaa aagttgaaag aaaatgaaga
    32521 atcctatgga caagagattc gacaaaaata cggaaaaaaa aaggttacag catccaatga
    32581 aaaatggatg aatatggata aagaaacgta tgaagaaatg caaactgttg aaaagcagat
    32641 gttatttgat ctcactcttt acttaaaaga accagaagat gaaagcttag cagaccgtat
    32701 cttccaagct cataagaagt ggttatctta cagctggcct gaatatcagc caaaagcgca
    32761 taaaggacta ggaatgatgt acgaagccga tgaacggttc actgcatatt atgacgagcg
    32821 aagtggtcat ggtgcagcaa aagctttaaa tgaaattatc caaaaaaagg cgtaatcttt
    32881 tacatgatcc aacaaaaaag tacacgtaaa atgacctaat aacaaaacag cagacaccca
    32941 agttagattt ttatgctaac ttttggtgtc tgctccattc tttcacaagt tttttcatcc
    33001 tactttaaat atattcatcg ttgaataaac gtataaggta ttttcacttt atcctttttt
    33061 tctgtaagaa acaattgaaa tgccatttct ccgcaagctt ccagatggtg gtcgatggtt
    33121 gggaaaccaa gaagctcgct gatcagcaaa ttatcgcgtc caatcaccac tggtgtctta
    33181 tcttgcagat agctttgcaa gatgcctgct gcaaccatat ccgaatttgt aaagacacca
    33241 tcaactccaa gctctttaaa aaagaagcct gcttgttttc ctttctctgc atctatacaa
    33301 tcccaaaaaa caagtgattc atcaaaagat ggaaaaagtt gatgacatag ctgcaaagtg
    33361 atttttgagt tataacttag cctgccactt cgccccaacg tgatgcccaa ttgctttata
    33421 ccttgctgct tcaaataagt aagaccttct gtaattgatt gttctcgatc aataaagaca
    33481 cagcttgcct gttttccctt gatttcttca caaaaaacta gaggtccata tttttgatat
    33541 gccaataaat catctagtgt attggcacga gacgtaatga tcaatccatc gaaagcttta
    33601 gctgcaaatt cttcaagata ccttcgctct ttttctttct gatagcccgt cggtaaaagt
    33661 gtcactttgt aatcataatc aaacgctgct tcaatgatcc cactgatcaa ctgatcaaaa
    33721 tacggatgat tggtataagg cagaatcact ccaatgcttt tcgtttttcc ataacttaac
    33781 tgtctggcat ttgcattagg gatataatcg acttctttca tgaccgcaag tatttctttc
    33841 cttttttcct catctacata tggatgtcca ttgattacac gtgaaatagt agaaactgaa
    33901 taacctgtga gctttgcgat atctcgaatg tttgccactg ctgcttcgcc acctatacaa
    33961 aaagattttc ttgtaaatag tctaccacag ctggaccgca ttcaaaaaac tgatacattt
    34021 ttccttcatg cgaaaaactg atccgctgcc cgccccaata tatatctttt gtcaaatttt
    34081 cgatttcatt cattggtatt tcttttacta tattcctatt caacccttga aaactgtaca
    34141 acgttaatac ggatcccttg aactcgacaa acgcataaaa ataattgttc gttgtgccat
    34201 ctgttatctc tacataagta ttcattgcaa gccctccttt tgatactttg agtatagtgt
    34261 atgaaacgcg tctcatagca aggagtttgt tttgccaaag aaaagtttta ttcctctttc
    34321 attattacca aagtctctta ttttaaattg aatacattat aatgatactg taaatatatc
    34381 tttattatta gtgaatggat atttactttt aatagataaa atattataac tattaaaaat
    34441 aactattttc attataatta caca
//
LOCUS       DACECR010000020        33934 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.020, whole genome shotgun sequence.
ACCESSION   DACECR010000020 DACECR010000000
VERSION     DACECR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.020"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1041..1706)
                     /locus_tag="IWO17_000897"
     CDS             complement(1041..1706)
                     /locus_tag="IWO17_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0289793.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1832..3868
                     /locus_tag="IWO17_000898"
     CDS             1832..3868
                     /locus_tag="IWO17_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ0289794.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3872..4510
                     /locus_tag="IWO17_000899"
     CDS             3872..4510
                     /locus_tag="IWO17_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0289795.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4538..4966
                     /locus_tag="IWO17_000900"
     CDS             4538..4966
                     /locus_tag="IWO17_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289796.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5423..6334
                     /locus_tag="IWO17_000901"
     CDS             5423..6334
                     /locus_tag="IWO17_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0289797.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6551..6988
                     /locus_tag="IWO17_000902"
     CDS             6551..6988
                     /locus_tag="IWO17_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289798.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7083..7967
                     /locus_tag="IWO17_000903"
     CDS             7083..7967
                     /locus_tag="IWO17_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0289799.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8191..11358
                     /locus_tag="IWO17_000904"
     CDS             8191..11358
                     /locus_tag="IWO17_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289800.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11378..11923
                     /locus_tag="IWO17_000905"
     CDS             11378..11923
                     /locus_tag="IWO17_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ0289801.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            11951..12406
                     /locus_tag="IWO17_000906"
     CDS             11951..12406
                     /locus_tag="IWO17_000906"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ0289802.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12403..13161
                     /locus_tag="IWO17_000907"
     CDS             12403..13161
                     /locus_tag="IWO17_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0289803.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13247..13987)
                     /locus_tag="IWO17_000908"
     CDS             complement(13247..13987)
                     /locus_tag="IWO17_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0289804.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14048..15301
                     /locus_tag="IWO17_000909"
     CDS             14048..15301
                     /locus_tag="IWO17_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0289805.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            15623..15850
                     /locus_tag="IWO17_000910"
     CDS             15623..15850
                     /locus_tag="IWO17_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0289806.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            15973..16299
                     /locus_tag="IWO17_000911"
     CDS             15973..16299
                     /locus_tag="IWO17_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0289807.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            16367..17080
                     /locus_tag="IWO17_000912"
     CDS             16367..17080
                     /locus_tag="IWO17_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0289808.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            17390..18241
                     /locus_tag="IWO17_000913"
     CDS             17390..18241
                     /locus_tag="IWO17_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0289809.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            18362..20227
                     /locus_tag="IWO17_000914"
     CDS             18362..20227
                     /locus_tag="IWO17_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0289810.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            20241..21683
                     /locus_tag="IWO17_000915"
     CDS             20241..21683
                     /locus_tag="IWO17_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0289811.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            21791..22492
                     /locus_tag="IWO17_000916"
     CDS             21791..22492
                     /locus_tag="IWO17_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0289812.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            22664..23143
                     /locus_tag="IWO17_000917"
                     /pseudo
     CDS             22664..23143
                     /locus_tag="IWO17_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            23154..23660
                     /locus_tag="IWO17_000918"
     CDS             23154..23660
                     /locus_tag="IWO17_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAQ0289813.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            24071..24646
                     /locus_tag="IWO17_000919"
     CDS             24071..24646
                     /locus_tag="IWO17_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ0289814.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            24646..25086
                     /locus_tag="IWO17_000920"
     CDS             24646..25086
                     /locus_tag="IWO17_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HAQ0289815.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            25102..25803
                     /locus_tag="IWO17_000921"
     CDS             25102..25803
                     /locus_tag="IWO17_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0289816.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(26301..26726)
                     /locus_tag="IWO17_000922"
     CDS             complement(26301..26726)
                     /locus_tag="IWO17_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289817.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            26957..28282
                     /locus_tag="IWO17_000923"
     CDS             26957..28282
                     /locus_tag="IWO17_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0289818.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            28284..30584
                     /locus_tag="IWO17_000924"
     CDS             28284..30584
                     /locus_tag="IWO17_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 3 glycosyl hydrolase"
                     /protein_id="HAQ0289819.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            30591..31562
                     /locus_tag="IWO17_000925"
     CDS             30591..31562
                     /locus_tag="IWO17_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0289820.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            31584..32981
                     /locus_tag="IWO17_000926"
     CDS             31584..32981
                     /locus_tag="IWO17_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0289821.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            33137..33760
                     /locus_tag="IWO17_000927"
     CDS             33137..33760
                     /locus_tag="IWO17_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289822.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
BASE COUNT        11403 a         5545 c         7073 g         9913 t
ORIGIN      
        1 acatcaaggt tcctagcctt tttataataa aatgaagcga aaataatttc ttttaaagag
       61 ccgagattat cagctatata agagtgaatt tgatgcaaaa aacagagccg aaacagtctt
      121 ggtgttcgta gctctgctta tatgtttcga atttaagaca aagagttggc aaaagatgtt
      181 gataaggaag ataagataac tgtgttatat aaactaggta actaactgta acaagctaat
      241 tttaagagat tcttattaga agacgtttag gaatataact aagaaaatga ttatacaggt
      301 aattgtcatt tcaataatgt tttctaattt tcgcgtcttt gttttaagaa aaaggccaga
      361 atcagttgga aaaaagttcg tacgatagtg aagaggggca aaatttccta ctaatattcc
      421 cactaacaag agataaaacg gccaggttgg tgtgattcct ctataaaaaa atgcccatgc
      481 taccgaaatc ccatagatta acaactgaac aatttttaca atgaaaaaat tgttctgcca
      541 ttactcaaag tctcttcctc cttgtatcgt ttatttgttt cattttatca ttatgatagg
      601 cgcttgaaca atccattttg tattttattg agtactcttg gataaactac cagcgattat
      661 catatttatt tatgttactg taagccatca tataaaagta aatgagcaga tcatagaaat
      721 ataaaaaaga actaaaattg atctaaatta tgtgcagctt gactacgtac caatctatat
      781 tacttactag attggtcgtt aatctattcg gatcgaatga gcaaaatatt tttgtgaatg
      841 tccatccttg attatgattc caaaaagaat cattgggagg ctgtggcgta aatattatac
      901 tataagccag aaatacctat cattctgaac ttttttattt aggaactttc atctcaaggc
      961 tttttcaatt aatttgtacc cctgtttttt ggataagatc atgtgcattg attttttcta
     1021 tctctacgtt catctcctac ttaatgccta tctggctcct gagggcttct ttttaaggcg
     1081 tcgtcatagt cttggctgag gatttcttgg taatctgagg gaatggcttc ccaagtgagc
     1141 aggcgaacgt tcatgacgtg ttttcctttg tgtttgatta cagcatggtt tttctctttt
     1201 tctacctctc cagcgtgaaa agacagccat gtaccatggc caaagatatc attacatacc
     1261 caacgttcga tggaattgtc cacgtatcct acagggagtg tggtatagcc gatttgtggt
     1321 ttcaaaaaag gattgaatcg atcgatcgtt tcatttttgt attgataatt gataagaaaa
     1381 agatagacca aacaaaagac gagcagaaag aaagatagtg tctttttgag tgagaagggt
     1441 tgttttgatg aataagattt cttgttaaca agaggttcat ctggcaatgt tggttctatt
     1501 gattcggtag ctgattcaat agaaggaaaa tcaatcggca gacctaaagt atgatgtaaa
     1561 agtgacagca tttcaaagtc tggcattgta cgagaatttt cccaactaga aatcgtttta
     1621 tcggaaacac caattttttc gcctaattct ttttgcgtaa gcttacaatt tctgcggtaa
     1681 gttcgtattt tttctccggt gttcatcttc ttctcctttc gattctacag tatatagatt
     1741 tattctacat gacctataca cttgatagat acgttttttt gatgaaaaac ggtatgattt
     1801 tatcaaagaa tcatttaagt aagggagagt ggtgataatc aaaaaaaagt tcacagtttt
     1861 actcacaatc atagtgaccg gtatcatgag tggttttgcc ttggttagta taaataaaca
     1921 ggtactctat cggacaaatg aatttttcca tcaaacagat acttatactc cgattcgatt
     1981 gccacaacat tttattgggc aagaagaata tgagcagcta atcatggtat ttgaaaatgt
     2041 ctcatcagaa actgagcttt cttatatgaa aaaaacagta aatgaatcca atgaagtaga
     2101 tacatcttgg aacttcaaga tacagccaaa gcttgaaatg acattgtatt atcaagggga
     2161 tgtcaaatct tctcacgaga aggtacccta ttctaatacc tcctttcgcg taacgtccat
     2221 tgaagaatcg atcaaaataa aagattttga aggtgaattt ttcttgatga cgagagaaaa
     2281 agaagcctat gaacgggtca tttcactatt cactgatcga ttcaatcaag catttcacac
     2341 tgattatgcc actaatattt ttgaagactt tgaaaatagc tattcgtatt cggttatttc
     2401 aaatacagac gtatataatt atcgagtgta tttaactatt ggattgattc ttttattcct
     2461 tttgttatca tcctttttct tattgatgaa taaagaaatc agtttattgt tgaataatga
     2521 cttttcatta catcaagtat tgtggtgttt attggggaaa aagaaaatca ttattacatt
     2581 cataattgca ggaatcattg gtggttttat ttttatgtat tataaggagc aatttcttta
     2641 tttgttccag atgtacgttt tcttgttcag cggaatatat ggtatctctt tgatttctct
     2701 tttattagtt gaaaaaatcg cttcgttaag agaagcgttc aactatatcc aatacattct
     2761 ttttacattt ggattgttgt tttctcttct tttgaccgtt gccaatgtcg aattagcaac
     2821 tatcgttttt tcttcttcac agttagctgt ttcccatgaa ccaccaaaag aaatcgctgg
     2881 tgaggactat catgtatttt atccagtaac catcgggaaa aaccacgtgg attttattta
     2941 ttccaatcgt tttgcatcag cagcagatca ggaattatat gaaactttga ataaaaacgg
     3001 aagtatatta gtcaatgtat cagattactt gaatgaagaa aatgaacagt ttggcagagg
     3061 gataaaaata aatctgaatt atcttaagaa atttcctttg attgatgtct ctggaaggtt
     3121 gatccaaata accgaaaaag aaacgcaccc tgttatattg atccctgaac gatatagaca
     3181 tacagattta aaaaatgacc tggcgcaaat aacagaatat tatcaagaaa tcagtgaaaa
     3241 agaaccgaaa tttctattta taaaaaacaa tcaacccatt tatacattta ttccttctat
     3301 cccctggatc gaacattatc cggttgttga aatactgaca ttgaaaaaca gtatgtattt
     3361 ggaaagaaat attctcagcg gagaaatcta tccaccgtta aagatcaaga taggctcttt
     3421 gtcgaaagaa aggttatttg agctgattga agaaagtcag ttacgtgata acttgcagtt
     3481 atcatttccg tatactcaga ctgaagagat agctgtcaaa gtgttaagca gatcatttca
     3541 ttacttgata cagatatttt tactcacttt cttttctttc ttcttgttga gctatgtcat
     3601 gttatgcata tattttgtct acaattgtcg gaaaatcgct atctttcgtt caagtggtta
     3661 ttcgctcttt gaaacgtata aagatttctt catgatgaac ctcatcaaat ggggaacgac
     3721 aagtgtaatc ttcctttttc ttatagaaag agagcctaaa tatcttttta atattttttt
     3781 ctttagcttc attgatttta ttctttcagt tgtttttatt ttgtctacag aaaagaaaag
     3841 tcagttattg ctgatgaatg gaggttaaca attggagccg ttgatagtga taaaaggtgc
     3901 gaaaaaaatc attcgaaaca aaacgattct tcatttggat cgtttcactg cggatgctgg
     3961 ggatcttgtg actgttgttg gagaaagtgg agcaggtaaa agtacgttat tgaatatatt
     4021 aggattgaat gatcagttca gttcaggaca attctatttg ttcgatcaag agacgaaaca
     4081 attaactggg agagagaagc gcttgataaa aagaaatacc atatcttttc tttttcaaga
     4141 ttttgggttg attgaatatg aaacaatcga ttttaattta gatattggat tgaagttttc
     4201 gaatttaaat gtttcagata agcgaacagc aaagtatgag gcattgaaaa aagtaaatat
     4261 aaacaagaag ctaagtacgc ctatctacac attatctggt ggtgagaagc aaagaatcgc
     4321 ctgtgcgaga gtcttattga agaatagcaa aatcatttta gccgatgaac cgactgggtc
     4381 attggataaa agaaataagg ataaagtagt tgaaatctta tcgcaacaag cttcccaagg
     4441 tacgttagtt atcgtagtca cgcatgatcc atatctgacc aaaataggaa acaagaagat
     4501 catgctttaa aaatcaaaaa ggaaaggatg tcgttttatg aaaaaagcag ttattatttg
     4561 tgcactaggg ataggttttg ttggaggaaa cgctatgtca tctgaagtgg aagcaaattc
     4621 agttcacggt gaaatctatt acaaatcttt aaagccagga gagattcatt ttgatgctaa
     4681 aggtaatatt attaagtcaa atttaccagt agaccgggaa atttctagaa cagcaattaa
     4741 tcatagctct ggaagatggg tttattatag tgatacaact tgtacgagaa aaacaggtca
     4801 ttctaaccac tatagtcgtg tctataatcg tcacggtgca aaagcgaaag ttggaaactc
     4861 tattcgatca gcaaatgcaa caaaaggagt atgggcctat gcaagtgcga caggaaatag
     4921 aactgacaca ttctcatgtt ggtacaatcc aaccggttgg tattaagtat ccaaaagttt
     4981 atataaaata gaaaaaacat ataatgaaac agcgttaaaa gttatttttg gtagttttaa
     5041 tatattcatt tttagttgta taaaaatata tcataataaa aacaacaaaa aaatgtctgg
     5101 aaaatcgaac ataaaagtgt ctgatttgaa tatcccagca gcggaagaag aaaaacaaaa
     5161 tcgataacat ttgttctaag aattagttac gttaaatacg gaacgcaaga tagacttgaa
     5221 aaacggtaac aaaagaagta gtatattaag gtgtgatgtg ctacttgaca tttattctgc
     5281 tgagcgaact ttctggattg tgtagcctta tatctattca ggacgtatcg agcagattag
     5341 taaaggtgtt cttgatgatt ttcatcttga acacctttgt ttttagcaat tcgcaaaaaa
     5401 cgaaaagtag gagaattgat aaatgaaaaa aagattttta gcacttgcca tcgtactagg
     5461 cactggctta ttgtcaggct gtacgaatgc cggtgaaaaa acagccgtta gttataaagg
     5521 cggtacaatc agcgaacaag aagtaatgga tagcttgaaa aaaatgcaag gagcagattc
     5581 cgctgtgcaa cagttgatcg tttatcaagt atttgaagat aaatatggag atgacgtatc
     5641 aaccaaagaa atcgattctc aatatgatca aacaaagaaa caattgggtg attcatttga
     5701 ttcacaattg aaatctgcag gttacacaga acaaacattc aaagacagta tcaaacaaag
     5761 tctagccttt caagaaggtc taaagaaaca tatcaaattg accgatgaag atttaaaaac
     5821 ggcttgggaa tctttccatc cagaagtgga agctcaaatt atccaagtag caagtgaaga
     5881 tgatgcaaaa gatgtgaaga aagctgctga taagggagac gatttctcta aattagccaa
     5941 agacaagtca actgatacta cgacaaaaga agatggcgga aaagtgaaat ttgattccac
     6001 tactacaacg gttccggcag aagtcaaaga agcagccttt aagttgaaag atggtcaagt
     6061 ttctgatgtg atcacttcta ccaatgcttc aacttatact acggaatatt atgtggttaa
     6121 aatggtgaaa aaccaaaaca aaggcaacga catggataaa tacaaaaaag aattgaaaga
     6181 aattgcaaca gacacaaaat taagtgacag taccttccag aataaagtaa ttggcgaagt
     6241 attgaaagac gctaatgtaa aaatcaaaga caaagacttt gaaaatgttt tgagtacatt
     6301 cacttcagat tctagtacag caagtagtaa ataagcggta taaataaaaa atatgaacta
     6361 tttaaatcaa taaaactgtt catctaaatc tttttattag aactaaatca acagtagttt
     6421 aattactact tgatttagtt ctttttattt taaaaatttt ttttacttag aatccagcga
     6481 ccctagatat ttttctgtat aatcttaaat aaagaaaaca atatcaaaaa atgcacgtag
     6541 aagtagataa atggagtggt tttgcaaatt tcttttattt ctagaagtta ttcacgtata
     6601 cggatttgtt tttctcacaa ataaaaaggc aggaagtaca atggatcatt tgtatataaa
     6661 atatattcat caattttccg ttttggtgtt aggcacacta attatagcgg ggattgtcgc
     6721 atttattatt caaggccgtg acatattaac agtacatcaa gagtttttca gttatgcatt
     6781 tggtattcct ctgatcgcta taaggtctga taacaaaaag tggaagaaac gctattacat
     6841 tctcttgcta tttttgtgtt tttatctgcc acctaagatt tttactttat taggtataca
     6901 atgcctatca ttttctttta tttcctatat cattgtttta attttcctca gtttcatagt
     6961 atggatgttt tatcttgtca aaaaataaat gaatgtttca tgaacagtac ttcatcacag
     7021 cctaattttt ttctttatgc tatcgttata ttaaagaaat caatcagaag ggaaagaaag
     7081 tgatgaaaaa agttttatta gtggtaaacc ccagttcagg cggtgaacaa gcaaaggaat
     7141 ttgagcaatt agcgatagca aaattggaat cggtgtttga tgaagtggtc gtcttgcata
     7201 caaaaaaagc aggggatgca aaaaacttta ctcgcgaagc ggctacggaa gggtatcata
     7261 gtgtgtttgt catgggcgga gatggaacag tcaacgaagg aatcagtgga atcgctgaac
     7321 aagaacatcg gccaaatttc ggatttttcc cattgggtac ggtgaatgac ttggctcgag
     7381 cattagggat accgctagaa cctgaggaag cgatcaatca tttttcgatt gaatcagtca
     7441 agccattgga tattggaaaa ataaatgacg actattttat gaatgtcgtc gcaattggct
     7501 ctatccctga atcaatcaat gatgtagatg cggagaagaa gacgaaattt ggaaaactag
     7561 cttatttcat gtcaggaatc aaacagttag cttccacaca atcctactcg tttcacgtag
     7621 aagttgatgg aaagaaggaa gaaattgaga gcagtacctt gctgatagga ctaactaact
     7681 cagtcggcgg ttttgaaaca ttacttccaa atgcaaaagt ggatgatggt aagctccatt
     7741 tggtttattt aaaagattct tctttgatcg atacgcttaa aacattgcca gacctattaa
     7801 aaggagtaga tgaatctacg aaaaatctag cctaccttac ttgtgagcaa ttagttgtgt
     7861 cattagctga tgaggaagaa ttgacgacga atgttgatgg agatgaaggg gataaattac
     7921 ctgtgacaat caaagtattg ccctcacatt tacaggttta ttgttaaaat ggaggttgtg
     7981 accgaggagt tggcgggaga acaccgcttg ttcgggataa gggactgtga caagactttt
     8041 gtcacagcct tttttttgta caaaaaagaa atttacacct aatagaaaaa tttttccata
     8101 aaaatacata ttttaaatat gaaagtacaa ttggaaatag attaaaacga taaaataaaa
     8161 ttgtcaaaac gtcagagagg atggtggaga atgaatcaat gtgtaaaaaa gctgattgcg
     8221 tcgattttgt ttgtcttccc tctcttgatg gtgacatccg tttttgccga tgtagagaaa
     8281 acaaacgaat caacgcaatc agagaatcaa agttttgaca agactacttt ttcagaagaa
     8341 tcgattactt ctgaaactac agaatctcaa agtgaggctt ctactaataa aaggcaagaa
     8401 gaaaataaga caagtgagac ctcagaagta gctgaacaaa cgtctgatag cagtgaagcg
     8461 gcactggcaa gagctgtttc agcggatggg tacagcgaag ctgccacaac agaagaactg
     8521 gcgcagcttc tcgcagacga atcagtaact aagatccgac tgattcagcc tttgacttta
     8581 gacagggaat tagagatcaa acgagatatc gttattgatt ttggtggatt cgctcacaat
     8641 ttcggcacac accatattta tatcaatgag atacctcagt cgatcgagtt ccagaatttt
     8701 aaaggaacag ctgcacatcc aggtggattg acaccaaata tcgatggaaa tgccattatc
     8761 attgcttata tgaccgattt ttggggaagc agatatcatt ttacgggaga aataaaattt
     8821 accgggacac ttgatttgta tgatgggtcc aaattaggac tgatttacgc tcctcgtgca
     8881 actgtcacat tagatggtgt ttccggtgtg ttggatgtcc aaccagtgaa ggaaggactg
     8941 aatgccgctc cgggtaaagc ctatttttgt agaagctatc aattgaatgt gatcaatggt
     9001 agtcaacttt atggaccgta tctagggaaa ttctacggtt ttctagattc aggagatgag
     9061 actggtagtg gaaaaacagc gccaggaatc catatcttaa gcggttcaaa ggtctctctt
     9121 gattatgatc gggcagatgg cgttagcgat gaaggagagg cagtcgatac gctacctggt
     9181 aatgtcatgt ttgaagtcag tggttcggga agcgaatttt cggtaaatac aaagatcaat
     9241 attaccaatg acaacaatcg cggaatcatc caaatgagag gttcaggatc gaaagtcact
     9301 gtatcgaatg gtgggaaaat caccatcaat acagcaacta cgggcggatt tcgcttgcaa
     9361 ggagattcta gtcaaatcca tgtatcaagt ggtggacaaa tctcggtcaa aatggctgga
     9421 gatgacgatc aacccgggaa caatgggatg cgttttgtag gaaaaggcct ttctttgatc
     9481 gtagaaggag ccggatcagt tatcgacatc gataagcaga gtgggcgttc cagcgcagtt
     9541 cgttttgaga atggtacgca aaagctgaca gtaaccaatg gaggctcatt gagggtaaga
     9601 aatacaggtg atggcaaatc ttacgtgaac agactcaatc aaggaaatca agcgatccaa
     9661 ttttatgacg cttctggttt tctagaaagt ccaggttcag ctgattttac agtcactgga
     9721 gaaaaatcaa atatcgatat acgcgcagat tccggagcaa ctgtagatac aactggagat
     9781 attgatttaa attttacggc aggtgaaaaa acttatttag tcatggtcgg gaaaacaggg
     9841 gaagataaga ccggcatcat cagtggggat gtgttgaatg tggacttaga atctcctacc
     9901 tattttgatt tccaaaacaa acggattgga agcgagacca atggcatagg cggctgggta
     9961 ttccaaggaa atggagattc ttctttaaag atcgcccatt cagaaatcgc tttatggcga
    10021 aaaacaggcg caacattcga taactttgaa aaagacccgg attatttttc tgatatgaca
    10081 gacttatcga tcaatgggac cgacttaggc aatttcgtat caagccctag tgcaggttta
    10141 acaaatgaat ttgctcagac aggtgccgga atgaaaaatt ataatcgaat cagtgccaat
    10201 aatcagctcc cgatgattga atccttaaga gtgccaacga atgcagataa aaaaatctat
    10261 gggcatgtcg ttgttcccga aggtgtcgag gcaaagccaa gagatgcttg gacaggcgaa
    10321 gtagaagtga ctttacgtat tacttacgca gatacgacga aatcatctgt agaaattact
    10381 gctctcacgc aaggacaaac agatgaaaat gaaggggcag gatataaccc atggggagaa
    10441 ggcgaacaag gcggcttgtt cgaagttcaa gtacccaatg atgagagatt aaaaacggag
    10501 gatcaagtgc aagttgttgc tgctaaaaaa gtaagaggtg gccaagaagt gacagaactg
    10561 gactctgcca aaacgacagt cgatgtgatt cctcctgaac ctgctaattt aagtaccaca
    10621 ttgatcaatg cagcttctcg cacgatcaca ggtacaggtg aagcaggagc cttggtttca
    10681 ttgaaaaaaa atcaagctgt tttgtcagaa acaactattg atgatacagg ggaatttgtt
    10741 ctttctatcc cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac
    10801 caagcaggag aagctactgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt
    10861 gggaatcaca atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct
    10921 gctacgaaga tcgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgatttt
    10981 ggtcagatca aagcaactgg tttgacacaa gaagcatttg gccaaatgga tatacaggaa
    11041 aaattatgga tttatgatca gcgagaaacg aaaaatccgt ggatcttaac gataaaatta
    11101 tctcagcctt tcacaaatga tgcaggattt tctatgcagg atgtcctata tttcaagaaa
    11161 ggagaggagt atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca
    11221 acgaatgtgc aagcagatta ttcagaatat ttaaataacg gtcgggcgtt taaacttgtc
    11281 ttaaccaaag aaaatcagat agctggtaat tactcagcag agataacatg gacattagca
    11341 gatgcgccga cagaataaag atcaaggagg atgattgatg aatcgaatat ggaataaaag
    11401 tcatcgaaac aactgctctt tacttatgat ccttctcctt ggattcatcc tttggcagac
    11461 acctgtccat gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat
    11521 tgataagaac aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc
    11581 ctacacggtc tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc
    11641 tacgacgaat tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac
    11701 attaagttat gatatgaaaa acttagcaaa aacagtggga gaaataactg ttcctcctca
    11761 aagtgcggtc actgagacac tttcactgaa agcgccagaa aaagagctgg ctggtgtggt
    11821 cgcaggaggg atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg
    11881 tgtcgtcaat cagttttctc aagtgaaagc aatcttgatt tgaaatagcg aaaaagagtc
    11941 ttccccagat ttgaaattaa aaaaagctta tgcggatcaa agcgatggaa gaaatgtgat
    12001 taaggcgaat cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga
    12061 aatctacaaa aaagaaacaa aagagcctta cctttcaata aaaaaagaaa aaatagatgt
    12121 agcacccaat acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg
    12181 acattatcaa gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga
    12241 ttttgaaata actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa
    12301 tcagccagaa acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt
    12361 atttgctgga ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca
    12421 ttaagtaccg ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat
    12481 aagagctcag ccactacaga aggaacgatt tctgtgacca aaccagatgc tgcaaatcct
    12541 gaagacgttg ttccagttcc tcctgttgat ccaaatgatc ctgatactcc ttttacacct
    12601 gacgaaccaa atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat
    12661 tttggaacaa tcaagttagg tacagcaaaa acagtatatg ctgcattaca aagcggaacg
    12721 gacagtactg gagcacaaac acaagtacag aatttcgtac aagtaacgga taaaagcgga
    12781 aattatgccg gttggaaatt atcagtcaaa cgaacagaat ttattggtac aacatctgat
    12841 acgaataaac taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca
    12901 actaatagca gtggaatgcc atctaccgtt tctggaactg gcgctgaagg aatcgaaatc
    12961 ccagcagatc aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg
    13021 atttattcat taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct
    13081 gtatccaatt atgctgtaga caactacgtt tcaacattca catggtcgtt aacagatgct
    13141 cctacagaag aggaagcgta aaatcatatc aacgacttca gggagtggag ccgattcaag
    13201 gcccactccc tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag
    13261 ttttttggaa gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta
    13321 aggagtcacc gttgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg
    13381 ataagttgat acagtaagat tttaaaagta aaaagtaagc atacttttct aactctgtct
    13441 tcaattgttt taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt
    13501 ggtttttcaa acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac
    13561 ttccaccttt ttgatcaata aagattttat cgacatttac gatctgctgt tctttccaat
    13621 tgatctgttc tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc
    13681 caatcggaag gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa
    13741 aaacaatatt actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga
    13801 gtgattgctt caagtcgata tcgataaaga aaacatcgtg attatcaata gtgcccaact
    13861 ggatcaactg attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat
    13921 tgaccaattc tctgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact
    13981 ttttcatatg caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag
    14041 gaataagatg gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg
    14101 tatctattta ttcttttcga aacaactgct agtaaaaaac agctttggtt gtatagcttt
    14161 gatggtggtg acacttttac ttcctatccc taaagtgagc tttccttacc taattggctt
    14221 attattgatg tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag
    14281 taatcttttt tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta
    14341 tttaggaaag agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt
    14401 ctatctatta gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta
    14461 tccaactgaa cactattttg gaggtatttg tattttatta agctggggcg tgatttactt
    14521 ggtattggga agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat
    14581 agtcatcagt tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga
    14641 aagacaacat caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct
    14701 agaggaattc aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga
    14761 aaataaaaaa gaaacgttgg aatttttaga aatattagaa gatcattctg cagctatttt
    14821 tgaaaaaccg caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt
    14881 tttaaaattt ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt
    14941 cgaagtgaaa acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat
    15001 ttctaacgca ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga
    15061 agaaaaagag ggatcacgtt ttagtatagc gaacaaggca aatccaacga atttgacgct
    15121 atcggagatg gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag
    15181 tgcgaaaaaa atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca
    15241 gaatcactat ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg
    15301 acttactgtc aaaggataag tttgaggaaa gtctacacgg agatagagac aacaacacag
    15361 catattcaaa agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg
    15421 tagttaaaca aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat
    15481 gatactaagt agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt
    15541 tgcttacacc agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagca
    15601 aatccctacg atcacaaaaa aaatgctgac ccagcagtta agagagttag aacaagatga
    15661 aagtatccat cgaaaagcct ataaccaagt cccgcctaaa gtagagtact cgctcaccga
    15721 agaaggaaaa tcattaagag agattctagt agcgttgtct gtttgggaag aagaacgtat
    15781 cgaaaaacaa aaagcctcaa gggaagaaga cacgctactg catacacatg gtggctacct
    15841 aaattattga ttttctatga catttatttt aaataaaaga aaacaggagt ttttttaaaa
    15901 gcatcatcta ttttttatgt aaaaaaatgg tatgattaat caagtaagaa gtgattggaa
    15961 gaaggcaagc aaatgaatag tatggaaaaa gtcaaccagt ttagagatga acgtaattgg
    16021 cgacaatttc ataacgaaaa agatttggct atctctattt ctttggaagc atctgaattg
    16081 ttagaacttt ttcaatggaa aacgccggaa gaagtaaaag aaacacaatt agaacgaatc
    16141 aaagaagaac tggcagatgt attgatctat tcatacatga tggcagacaa ccttcacctg
    16201 gatctagatg agatcattga agaaaaattg atcaaaaata atttgaaata tcctgtagaa
    16261 aaaagcgcag gtgtacgaaa aaaatacaca gagttgtaga aatgtgtcat tcggctgttg
    16321 gaaagagcaa aagaaaaaca gtaaaatgag tctggaggga gagaaaatga aaaaaaaagg
    16381 aaaagcaaaa agatggctag tcaatatcct cttgttcctc ttgttattag taggactagc
    16441 tttgattttc aatgaacaga tcaaggatta ttttgtcaga gagacaggag ataaatatgc
    16501 tatagcgaat gtaacaaaag aagatctgaa aaagaataac gataaagatg tcagttttga
    16561 ctttgatgcg gtagaaccaa tgacgacaga aggggtcatg aggtcgcaaa tgagaggaac
    16621 agatctacca gtgattgcta gtatcgcagt cccttcagtt tcaatcaatt taccggtttt
    16681 caaaggcttg gataatacgt cactacttta cggagcagga acattatctc cagatcaaga
    16741 aatgggaaaa ggaaattatg ccttagcaag ccatcgtgca acgaaccctg aattgctgtt
    16801 tacaccgcta gaaaatttag agatgggtgc caagatctat ttgacagatt tagaaaatgt
    16861 gtatacatac aaaacatttt ttaaagaaaa agttgctcct acagataccc aattactgaa
    16921 tgaagtcgag ggaaaagaaa ttgtcacact aattacttgt ggcgacatgg acgccgtcac
    16981 gcgactagtt gtacaaggag aactggaaag tgttacatcg ataaaagatg cgacagatga
    17041 tatgagatct gctttcaatc tagagacaaa aacattttag gatactggga cataagtcgt
    17101 tacagattaa aaacagccaa aggatatgaa tcgataagga aaaaccaatt tgctgaaaaa
    17161 aagagaaacg caaagaggtg catctctgat ttggttgttt tctagataga cttgtgttcc
    17221 agttttttta ttttttatat gaaaaggctt gcataaataa aaattcgatg gtatgatgat
    17281 tttgaataag aaatggattg ttactgtacg gcaggcaaaa ccagaattca gctaagaaac
    17341 agtaggcgaa ttctaggttt ttttattttt cttgagaggg cgaagcaaaa tgaaaattga
    17401 taaaattttg aataacaatg tcgtcatttc gaaaaatgga ttcggggaag aagtcgtttg
    17461 tatgggaaaa ggactagctt ttcaaaaaaa gattggggac gagatttcgc cagaagcggt
    17521 ccaaaaagaa tttgttttaa gagattcttt tgctaaaaac caattccagc agctgatggc
    17581 tgatattccg gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa
    17641 actaaaagtt gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc
    17701 gatcacacga gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa
    17761 attttatccg aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac
    17821 gggtgtggaa ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca
    17881 acaaggaaac gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact
    17941 gagtatcatc agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg
    18001 tatcattact catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga
    18061 gcaagatgaa tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt
    18121 cgaacggatc aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca
    18181 gatttattta acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata
    18241 aggattgtta ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg
    18301 tgactgtttt tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac
    18361 aatggataat caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt
    18421 caaaagttta gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc
    18481 agatacgcaa aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca
    18541 gtatcaagta gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga
    18601 aggtcttgca gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat
    18661 cgatattatc tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt
    18721 gaaaggattc ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta
    18781 tcaaatattg tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt
    18841 tacagcagct aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact
    18901 tgtgtatcca gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat
    18961 cccagtgatt ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt
    19021 atgggttcaa agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat
    19081 gattttagtt ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg
    19141 accaatcggg acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt
    19201 tagtccaatc gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg
    19261 catgcattgg ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt
    19321 attgatgcca atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc
    19381 attgcgaaca aaagatacga aacttcgagc attagggatt tcttctacag tgacttcgtt
    19441 atttggaatc actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat
    19501 tgccgcttgt atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc
    19561 tttctccagc agtttggtca gcttattgac gattccgact ttcattaata cggtggatgg
    19621 tgtagagtca aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt
    19681 cgtaggtaca ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa
    19741 acatgcgaat gcaggagaac cgattacttc agcacggcat accttaaaaa gtcctttaac
    19801 gggtaaagta ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg
    19861 aaaaggtatc gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat
    19921 cacgactatt tttccaactg gacatgctgt agggatcaca acgactgacg gagcggagat
    19981 tttgattcat atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt
    20041 caaacaaggg gatctcgtca aagcaggaga tttattgatt cgttttgaca tcgaagcaat
    20101 tagagcagct ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc
    20161 cgatatactt gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt
    20221 aaaataagga ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag
    20281 caactgctgc caatcaatta gaaggggctt atgatcgaga cggaaaaggg ctatccgttg
    20341 cagatgctat gcctggaggt aagcagcgct ttgcgattat cggcagtgaa gaatttgact
    20401 ggacgattga tcaagaaaaa tatatttacc cgaatcatcg aggaattgat cattacgatc
    20461 gtttcaaaga agatcttgcc ttgtttgcgg aaatgggatt caaatgttat cgtttttcga
    20521 ttgcctggac acgaattttt ccaaatggag acgaaggtac tcctaacgaa gcgggattag
    20581 aattttatga tcagctgatt gatgagtgct taaaatatga tattgaacct gtgatcacga
    20641 tttctcatta cgaaatgcct cttcatttgg caaaagaata cggtggctgg aaaaatcgaa
    20701 aactgatcga cttctatgaa cgatttgctc agacagtatt agagagatac agcagtaaag
    20761 tcaaatattg gatgactttc aatgaaatca attcggcctt tcactttcca gctttaagtc
    20821 aaggattagt aaaaagcaat ggagcaggag agtaccaaaa tattttccaa gcatggcata
    20881 atcaattcgt tgcaagtagc aaagccgtaa aaatcggcca cgaattgcgt tcggatatcc
    20941 aaatcggctg catgattatc tatgcaacaa cttacagcat cgatgccaat ccagtcaatc
    21001 aggcagcaac aatgatccaa aaccaagaat tcaatttttt ctgcacggat gtccaagtac
    21061 gtggagaata ccctgcatac acagcgcgta cccataaaaa atatggagtc gatccggaaa
    21121 aattagagca aacggaaaaa gacttccagt tgctaaaaga atatccagtg gactacatcg
    21181 gatttagtta ttatatgtct acggcaatca atgagactga tccacaagca gcaacttcag
    21241 aagggaactt gctgggagga gtaaaaaatc cgtttcttga ggcaagtgaa tggggctggc
    21301 agatcgatcc agaagggctg agaatcgcat tgaatgaatt atacaatcgt taccagaaac
    21361 cattattcat tgttgaaaac ggtcttggag ccattgatca tatggaagag gatggcacga
    21421 ttattgatga ttatcggatc gattatttac gtcgtcatat cgaagcgatg gctaatgcag
    21481 tagcagacgg tgtagatttg atgggctata cgccttgggg atgtatggat cttgtcagtg
    21541 catcaacagg agaaatgagt aaacgttacg gctttatcta tgtggatcta gacgatgaag
    21601 gaaatgggac actgaatcga tcaaagaaaa aatcttttga ttggtataaa gaagtaattg
    21661 cctcaaatgg cgtaaacttg taattaaagc agctgaatga aaaagaacac acttctcaca
    21721 tgtgatttgt gttctttttt tattttttat gtgctattct tttagtaata gtaaagcaaa
    21781 gggtgacgtt atgaaaagga cacgtgtact atacttggaa gtcgcagata aaattaaaga
    21841 agatattttc tcaggaaaat atccagtcgg ttcgatgtta ccgacagaaa gtgaattaga
    21901 agaactgttt caagtcagca aaatcactgt ccgaaaggca atcgaactgc tggcatcaga
    21961 tgaatatgtc gcaaagaaaa gtggtcgtgg gacgacggtt ctaagcaatc gtccttacaa
    22021 tcgattatcc aaagcggcaa gctttacgca aatcttggag aactcccgat atgaagtccg
    22081 gaaagaaacg ctggatttgc aattggtcca tataaagaag gaagatcctt tgttttcctt
    22141 tttcggtgag caagctgttt gttttaagcg cttatattat ttgaacaatc agccttatat
    22201 ttattttgtt cattatcttc cacaagaaat gatgcaattc agcagagaag aatttggcaa
    22261 agaatctttg tatcgtttgc tgacgaaaaa aggatatact attgactcat tttctgatga
    22321 tttctcggca gtatttttaa cagaagaaga acaagaaatt ctgaaaacag acgcacagat
    22381 cggcatcaaa cggatacgga aatcgtctac gcaaattggg gaaatgattg aatattcgga
    22441 ggcggtctac catacggagc tgcatcctta ccatattgat tatgaaacat aaataaaaaa
    22501 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctt
    22561 ctcatattag cttctcatat ttttcgtttg gttaggctta ttaccgcaga tacccggctg
    22621 tttctggatt accatttatt ctggataagg gggtgtggca cgattttcag aaggaatcta
    22681 tgaaccaatc actgtattga ttttagcttc gatggtaacc ccattcggct tgacgatcgc
    22741 ttatttctta gggaaaatca ttcgtaaaaa tatattcaat cgtcaagaaa tcgatacatt
    22801 gaagaccgct tttcctatgg ggatctgtca gatcacagag ggatgtttcc ctatcgtatt
    22861 gaacgacttg gcacgcaatg tcatagcaac gggagtcggt ggggcagtcg gcggtggatt
    22921 gagcatgttt tggggcgcag atagccgtat tccggcatcg ggaatgtttg cagtcgctac
    22981 gatgaccaga ccatgggcat tcattggtgc attgttagct ggttcttttg ctacgggaat
    23041 aactatttta ttattgaaaa aaccagttga cccaaatgca gaagtcatcc aagaagaaaa
    23101 agaggaagaa gagatatctt gggatgactt gacaatcagt taggaggaac agaatggatt
    23161 acaagaaaag agtacaacaa atgttcctag attcaggagt agtattgaaa gaaacagaat
    23221 tagaaagaat cgaatataca gattttggat taggagcaat cgagactgaa ggattgaatt
    23281 tatttgttta tgaaaataat gagcgatact gtgggaaaga actggtatta ttgccttatc
    23341 aaacttgtcc agagcatcgg cacccacctc gagatgagaa tgaaggaaaa aaggaaacct
    23401 ttcgttgtcg aaaaggctta gtatatcttt atgtcgaagg aaaagaaaca aaaaaacttt
    23461 cctgtattat tccaaagggg caagaacgtt actatacggc aaaaaaagaa atcatacttc
    23521 acgctggaga tcagtacacg atcgaacaaa atattaaaca ctggttccag gcaggtgcaa
    23581 caggcgcggt tatcaccgaa ttttcttcca gcagtgatga tccaagtgat agttttacga
    23641 atccagctat ttatcgttaa tacaaaggtt tgggaacaag ctgatgaacc aaaatgatca
    23701 aatttcgatt atttggttcg tcggcttttt tatttagaga aacatcaggc gttggtgaaa
    23761 cataacacga gaataaaatg gatttttcat taataggaga aacaagtgtg tgactattta
    23821 aataatgcgt gttattgaca tgcaggacga aatcccaatt tcttctataa gtaagtcgtt
    23881 tcaattttat atttactagt tatttatttt tttggttact gtaaaatagc tgaaaaatta
    23941 tttttttgca aatactatat atagtggtgt ttgatctata taccactaaa aaactacaaa
    24001 atctagtctt ttattttaaa aaaacacgtg tagaatgaag atagactttt gagttaggga
    24061 gtgttttttt atgaaactta ctgttttttg cggttcccgt tttggcaata aagaaagtta
    24121 caaatttatt gcgcaaacgt taggaaaata tatggcacaa gaagaaatcg aactggttta
    24181 cggtggttca tactccggga ttatgggagt gattagtaaa acagtactgg aaaataacgg
    24241 aaaagtcaca ggtatctatc caaatggcct ttttgaaact gagctgcctg ggaaagatgt
    24301 tactacattt attcctacag agacaattga tgaaagaaag gtattattat ttgaaaaagg
    24361 agatgctgtc ctcgttttcc ctggaggtct aggtacattg gaggaatttt cgcaacttct
    24421 ttcttggata gcgatcggcc tgacacctga taagccaatc ggaatattga atattggcgg
    24481 ttattacagc ggcttgcaac aattattaga aacttttgca aaagaggatt ttatggataa
    24541 aaaatggttg gatcaagtga tcttttccaa caatccattg gaactggtca atgaattacg
    24601 agcagtatct attaaaaatc agttattatt gaaggaggca aaataatgag tcaaatatat
    24661 ttagcaggac catttttttc agaggagcaa atcgatcgag taagtcgtat cgaaaaagcg
    24721 ttggaggaaa acaaaacagt cacaagtttc tactctccac gtcatcacca agaaagcaat
    24781 tatgaactat tcagcgctgg atgggcgcag gaagtatacg aaaaagatat ggaagagttg
    24841 acaaatgcag aatttgtttt ggctatcctt gactttgagc atcaaacaat cgatccagga
    24901 acggcttacg aattaggtgt ggcaactatg ttgaaaaaac cgatgatcat cgtacaagaa
    24961 gagacggtac ctacgaactt gatgattaca caaagtcttc acacgtattt aaaatcagac
    25021 caagctgtac gtgaatatga ttttgaaaaa ttgcctgttg aaacttacgt aggcgagtac
    25081 ttatagaaga ggaagggttg tatgtcagtg attttgattt caggaacgat tggtgcaggt
    25141 aagagcagtt tgacggatat gcttgcaaaa gagattgatt ctaaaccatt ttatgaaaat
    25201 gtcgaagata acgaagtatt gccgcttttt tattcaaatc ctgagcagta cgcattttta
    25261 ttgcaaatct tctttttgaa taagcgattt ttagcaatga aaaatgcttt agtgaacgat
    25321 gacaatgtgc tagaccgctc catctacgaa gatagtctgt tgttccatct aaatgctgat
    25381 ttaggtcggg taacggatat cgaagtacag cagtacgata gcttattaga tacgatgcta
    25441 aacgagttag atgatgttgc tccaaaaaaa cgtcctgatt tgatggtcca tatcaaagtt
    25501 tctttggaca cgatgctaga acgtatcaaa aaacgcgggc gtgactatga acagctagaa
    25561 agcgatgaaa cgttatacac ctattacgaa acactgaaca cccgctataa tcaatggtat
    25621 gaggattttg atgtatgtcc taaaatacaa gtggatgggg atacattcga ttttgtagaa
    25681 aatccagaag atgcaaaaaa ggtgattcgt cagatccaag aaaaattagc tgaattggaa
    25741 ggaaagcctg ctggaaaata ttttacagat aatcagcatg cgaaaggtca agaattaggc
    25801 taaaagtaaa aaaccaaacg acgggaaaga aaaattacta cgtgatgtag aataagtctt
    25861 tctatcgttt ggttatttta tattcaaaga tgtgaaataa ataaaacgtt actctttgat
    25921 ttgtgaactg aaatgatcca ctgtttgttt tactgattcc agtttttgaa gcttgatcaa
    25981 aggaagaagc tgagtttgaa gttcataaaa atcagccata gtgagttttg cttgattttg
    26041 cttatcttct tttgcttttt acataatttt tgacaggata agattcaaac gatttaaaag
    26101 aatctttgac tagtttgtgg caattgtcgc atatactcaa acttccttga tcattaaatt
    26161 ctgtatggat atttttgttg ttattacatg tacaataatc aggactatcg atcatccatt
    26221 tcgtctcctt tacagtcatc ttgcttcttt actatagcag aaaagaaggg gagaaacaaa
    26281 agaagatcca acagatggca ctatagactg tagatgttgc catccttatc cttcaaacgg
    26341 taattaccga ttttggtaaa tgtgactgtt gcttcttttt tattttcttt tcctgaaata
    26401 atatattcgt catctttggt agtaatcttg atatctgtca gcactgtttt ttcttcagat
    26461 gattcagaag aatcagctcc ttgtgtctga tacgtgcgtg tccattccat ttttttatct
    26521 gtaaatgtca actgtcccgc ggataaatcc atatctttac gtacttttag cgtatataca
    26581 ctgttggtga tatctgcttt ttccgctttt aagacagaag aatcggttgt ttcgtccgag
    26641 gattggtcag agccgccaaa caaatcacag ccagtcaatg tcaaaagtcc cgcagacaaa
    26701 ataaatagta cgattaattt cttcaaaata cttcccccat ttgataggat aaagaaatta
    26761 taccattgga aagtaaaact tcaaacggat ggaagagaaa aaattcacaa aataccatta
    26821 aatagaataa tttttcaaaa gtcataaaaa atccaccacc tttagaaagc gttttaaaaa
    26881 aatgtgttgc aatctttaaa aaaagtgcta tattaaaaat gaaccggttc aattttagaa
    26941 tctaggagat gaaataatga aagtcgtaga cagactatct gaaaaaatgc taccaatcgt
    27001 atctaagatg agtagccagc gacacttgct ggccattcga gacagtttta tcgttactat
    27061 gccgttagta atggctgctt ctgtcatggt tttgctgaat gctctgattt tttcaaatgc
    27121 aacagtccaa agaatacttg atctaagtcc attggctgaa cttgcttcaa tcgtgaataa
    27181 tggtacgatg agtattttag ctattatcgt gtgttacaat atcggctgga atttggcaac
    27241 ccactatatc caaacaggcg tgattgatga tccatcattt agtcctgttc atgcaggaat
    27301 tctaagtaca gccgtaatgt ttattttgat gtctttatca agtacagtca ctttgatgga
    27361 cggaaataca gcagaagcta caggggtttt cttacaaagt ctgacttctt cgtctggctt
    27421 agcaacggcg atgattgctg cccttgtcag tacagaatta tttatcaaat tagcgaaaat
    27481 gaagagtttt aaaatcagga tgccagaagg agttccaccg gcagtagctg cgtcatttaa
    27541 ttctttagta ccagaaatag ttgtcatttt atttttttca gttgtcgttt ttactttaaa
    27601 ccatacgact ggtttgaatg ttccgcaatt aattgaatta gcgattcaga caccattgaa
    27661 agcttttgtt ttatccgtcc caggcatctt atttttacag tttttctctg atttcttatg
    27721 ggtgtttggt atgcatggtt cgagtatttt ggctcctata cggcaagcac ctttgttaca
    27781 gtctattcag gaaaacatgg atgctttttc agtaggaaaa gaaattccta atattattac
    27841 ggagcccttt accaatgcat ttggattgat cggaggtggc gggtgtattt taccgttagt
    27901 tattgctatc ttgtgggctt ctaaacgtca agaacagcga tcaatcgcta aatttggtct
    27961 gactacttgt ttatttaata ttacggaacc aattatgttt ggattaccag tagtcatgaa
    28021 tccaatatat atgattccat gcgctattat accatctatt aacttggtta ttgcctacgc
    28081 agccacaagt cttggaatca tcagtaaaac agttgctgct gcaccttgga tcactccacc
    28141 ggttatacaa tcttttattg ctacaggagg cgatatacga gcagcagtct taacagtaat
    28201 acttatcatt ctagatgtat ttttattcct gccatttgta ttagccgcta ataaggcaaa
    28261 attggcagaa ggaggatatt aaggtgtacg attacccgta tcaaaaccca aagttaacca
    28321 tccgccaaag agtagaagat ttactttcga ggatgaatat tgctgaaaaa gtaggacagg
    28381 taaaccagca tttgtatggt tgggaatgtt atgaaaaaaa cggagacaag atcgaattaa
    28441 cagaaaagtt aaaagaacat gttaaatggg gaggcggtct gggtgcctta tacggtctgt
    28501 ttcgctctga tccgtggtct aaaacagatt ataagaatgg gattcctgca aaagaaagct
    28561 ggaaggtggc taacctagtc caagaatatg tactacagca ttcgcgatgg aaaattccag
    28621 ttttgcttgt agaagaatgt ccacatggtc atcaaggctt ggatgggatt tcttatccaa
    28681 cgaatatcgg ccgtggaaat actttcaatg tagcattatt agaacaatcg agtcgattga
    28741 tggcaaaaga actggctgct aaaggtgttc atttagcgct ggtgtcgact ttggatctgg
    28801 caaaagatcc tcgttgggga aggacagaag agtgctttgg ggaagatccg attctctcag
    28861 ctcgtttttc tgaagcggtc gttcgagggt tccaaggaga gattatttca gaaaaagttt
    28921 cttttcttga tcagacagta gaggaaatca ataagaagaa ggatcagatc ggtgttgtgt
    28981 taaagcactg tatcgcacaa ggagaagctt taggcggtca taattcaggc acagtaacga
    29041 ttggacgcag agagttctcc gatatttacg gaccattatt gaagagtaca agaaatgcag
    29101 taggtgtcat ggctgcttat aatgacatcg acgggatacc ttgtcatatc aatcgagcgc
    29161 tttttgagca aacattaaga aaagagatcg gttatcaagg aatcgttatg gcagacggtg
    29221 tggctttgga tcgtttatcc gatgttttta cagataaaaa gactgctgca gcatatgcat
    29281 tagcggcagg aatcgatctc agtttgtggg atgagaccta taccaagatt gcagaagcca
    29341 ttgataatca agtcgtagat gaaaagctac tagatcaagc agttcgccga gttttatctg
    29401 tcaaattttt attaggttta tttgaacagc catttgcaaa cgatccaaag gagtattggg
    29461 atcatctcat ggaggaatca gagcacttaa atttagagac agcaaaagaa agtatcactt
    29521 tactaaaaaa tgacggtata ttgccgttgg atcaacaagt aaaaaacatt gctgttatag
    29581 gtccaaatgc tcacgatgtt taccacttgt tgggagacta ctctgcacca caaacagaag
    29641 accgtttgaa aaaaaagata gtacaagaga taaaaacaga gtttccccaa tcaaaggttt
    29701 cttatgcaca aggatgtgaa gtaagaaatc aggaggatca ggaagaaaaa ttattagaag
    29761 cggtgaccct tgctcaaaac tcagatgtga tcattgcagt tctaggtgga tcaagtgcgc
    29821 ggaactttga tatggaattt cttcgtaacg gggcagtttc atcgaaaggg atcaacatgg
    29881 attctggaga aaatgtagat gttgcttctc tttctttagg tggataccaa gaacagctga
    29941 ttgaacaatt gtatcaacta ggcaagccaa tcatcacgat acttgtgcaa ggaagaccct
    30001 acgatttgat gaaaatcgag tcatttacga atgccattgc aacagcatgg tttcctggac
    30061 aggagggtgg aaaagccatt gcgcagatga tttctggaag aaataaccca agcggacgat
    30121 tgagtttgtc ttatcctaga aatagtcggc aactacctgt ttattactat cagcgtgctg
    30181 cttctaaaca agagaattat tatgatttaa gcggaagtcc attttatcct tttggacatg
    30241 gtctaagcta tacaacattt gaatacagtc agatggaagt caaaacggat attgacaaag
    30301 taataatatc aattaaagtt aaaaatacag gaagtgtttc aggtaaaaca agtactcttg
    30361 tctatgtccg attaattgac ggcgttgtca tccaaagaac gaaattattg aaagcttttc
    30421 gtaaagatga attgcggtct caagaggaaa gactgcttgt ttttgaactc acttctgaag
    30481 actttagtta tatggatatt gatgacaagc ggcattatgc aaagaaagcg gtcatcatgg
    30541 tcgaacaaca agaaaaagaa atcaaatgta actggcagga gtgaaaatgt atggcatcga
    30601 tccgtgacgt ggctaagatg gctggtgttt cagtgggaac agtttcccgc tatttaaacg
    30661 gtcagcagct gaaggaaaag aatatggaaa aaatatcaga agcgatttca gcgttggact
    30721 ataaagaaaa catcattgct aaaggattga agaataatcg aagtttctct attggactgt
    30781 tgataaacgg gatatctaac cgatttggtt cggaggttgt ttcagggatc gaaagagtag
    30841 ctgaagaaaa tgggtatagc ttattattaa gcgggtttgc cgatcagcca gaaaaaatcg
    30901 aacaaaaaat cgaatacctg atgaagcatg cgatcgatgg attgatcgtc ttcttatcag
    30961 aagaagagtg gtctggattt gaaatgctga caaaaatgag tatcccagtt gtcgcattga
    31021 attgtccggg tgggccagca ggtattgata caatagtagt gaatgatcga gaaagtgtag
    31081 caaaagtgat tgctcatcat ggcggacaag gacataaaac aataggattt attgctgccg
    31141 ctcaaacaga ttacgtagca agagaacgtt tagctggagc caaagaagcg gtacaaaagg
    31201 atccgtctat ccatttagag atctttacag gagactattc tagaaaaagc ggctattacg
    31261 gggcaaaagc attattagaa aaaggggcaa ctgctatttt tgtcagcaat tacaacatgt
    31321 cgattggtgc catcgaatgt ttcaatcaag aaggtgtcag aatcgggaag gatattgcgt
    31381 tcagtcacta tgattattta gataaaaacg atctgagtat ccttccaaaa atcacgatca
    31441 cgccaccaac agataaaatc ggggaacgag cagcagaaca gctgttgaaa cgaatcaaaa
    31501 aagaagagga agaaaatgaa aaagtgattg tgatggaaaa tatcatacat ggaatcaact
    31561 aaaaaagaaa aagaggtgct ttgatgaaaa gatatcaatt tccaaaaaat ttttggtggg
    31621 gctctgcagc aagtggtcca caaacagaag gaagaatttc aggcgatgga aaaggagaga
    31681 atatttttga ttattggtac aaaaacgaac cgcaaaagtt ctttgatcaa gtaggacctg
    31741 aaaaaacctc gcaagtttat acgaagtatc aagaggatgt acagttgatg aaacagactg
    31801 ggcataattc atttcgaacc tctatccaat ggagccgttt gatacctgat gaatctggaa
    31861 caatcaacga agaagccgtt cgtttctatc atcactattt tgatgagttg attgaaaacg
    31921 gtatcgaacc ctttgttaat ctgtatcatt tcgatatgcc gatgtatctg caagaaaaag
    31981 gcggatggtt gaaccgagaa acggttgaag cctatgaaaa atacgcaaaa agttgttttc
    32041 aattgtttgg ctcgaaagta aaaaaatggt ttacccataa tgaaccaatc gttccagtcg
    32101 aggcaggcta tttgtatcga tggcattatc cggaagaatc agatatgaaa aaggcaatcc
    32161 aagttggtta tcatgaagcg ttggcttcag cattagccat caaagcttac catgaaatgg
    32221 atcaaagcgg tgtgatcgga atcatcttaa atttgacacc gagttatcca agagacgaat
    32281 caaatcccga agatgttaaa gcagcacgaa tagcagatgc gttcttcaat cgttcattcc
    32341 ttgatccagc tgtaaaaggc gagtttccca gtgaattgat tgagagaata aaagaactcg
    32401 atctaatgcc ggagattcat ccagatgatt tgacgatcat taaagaaaac acagtagatc
    32461 ttttgggaat taattattac caaccacgcc gaataaaagc aaaagaaaca ccaatcgata
    32521 ctaaaaacgg tcctatgcct gaggattact tcgacaatta tgaaatgcct ggtcgtaaaa
    32581 tgaatcctta tcgcggatgg gagatctatg aaaaaggaat ctatgatatt ttgatcaatg
    32641 tacgagatca ttatgggaat atcgactgtt ttatttctga aaacggcatg ggtgtagaaa
    32701 atgaagcacg atttatcaag gaagatggta tgatccacga tgattatcgg atcgaatttg
    32761 ttcaatctca tctgaaatat gtccatcaag ccattcaaga aggtgccaat tgtttaggtt
    32821 atcatatgtg gacatgcatg gataactggt cgtggaccaa tgcctataag aatcgttacg
    32881 gttttatttc agtagattta gcacaagatg gaaaaagaac gatcaagaaa tcgggtcatt
    32941 ggttcaaaga tgtatcagac agaaatggat ttgaagctta aagaatagcc aagagagaat
    33001 gtatgaaaat tcccgttctt accgcttcag gaaaaagaag ttcaattatt tacgatcttt
    33061 ttcaactttt ttgtctttct atttcatttt ttgtatataa tagaatagtc aaaattaaat
    33121 aggagtggag gaaggcatga ggaaaaaaag accgtttctt ctcttttggt tattcggttt
    33181 tttcttactg cttattttat ccgcagcatt ttcaggaaaa atgacagcag atcttgctca
    33241 gaaagaaaca gaaaaaatcg tagcaagcaa agaatcagta gataatttca aaaaagaatt
    33301 ctcaacttat tggtgggagg aatataaatt aactgccaat tacgaaaaag gtgaattgga
    33361 catctatttg ccaaaaggaa aagaagaaaa tcaatttatc aatatcgatg acgtacttag
    33421 tgcgatttct ctcagacaat atcaattcaa agacaaacat ttggtcgttt tgcttttgac
    33481 tcatgatggc gacaaggtag cacgtttgaa aaatggaaaa gtggttacag cttattctga
    33541 aaaagtggac aaaaaagtgg tggccgatcg actagcggaa tggaaaaaga ccaacgaaca
    33601 agtcaagaaa acaactgctt cttctcaaaa aaacgagtcg acagaactaa gcccttcaga
    33661 ttctggcagt ggacaaacga taacgggaaa tgaagaaaat caagcctcaa tcattacgaa
    33721 tgagtcatca gaaggacaat atcaaaccta tggacaatga aaaacagaag ttgtgacaga
    33781 agtggtcagc ttcaagaaat aagaagaaat ttacgaaaag tgcttctcaa gtttttgtaa
    33841 aatttcggct tatttccgaa gaagctactt ctggaacacc gtgaatcaga ggttgtgaaa
    33901 tcagcggttt gacctgagta ttagagaaaa aaac
//
LOCUS       DACECR010000021        33719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.021, whole genome shotgun sequence.
ACCESSION   DACECR010000021 DACECR010000000
VERSION     DACECR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.021"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(7..963)
                     /locus_tag="IWO17_000928"
     CDS             complement(7..963)
                     /locus_tag="IWO17_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0289823.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            1137..1697
                     /locus_tag="IWO17_000929"
     CDS             1137..1697
                     /locus_tag="IWO17_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289824.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            1690..2515
                     /locus_tag="IWO17_000930"
                     /pseudo
     CDS             1690..2515
                     /locus_tag="IWO17_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
     gene            2525..3142
                     /locus_tag="IWO17_000931"
     CDS             2525..3142
                     /locus_tag="IWO17_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0289825.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            3160..3843
                     /locus_tag="IWO17_000932"
     CDS             3160..3843
                     /locus_tag="IWO17_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0289826.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            4526..5809
                     /locus_tag="IWO17_000933"
     CDS             4526..5809
                     /locus_tag="IWO17_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0289827.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            5825..6712
                     /locus_tag="IWO17_000934"
     CDS             5825..6712
                     /locus_tag="IWO17_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0289828.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            6729..7565
                     /locus_tag="IWO17_000935"
     CDS             6729..7565
                     /locus_tag="IWO17_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0289829.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            7568..8191
                     /locus_tag="IWO17_000936"
     CDS             7568..8191
                     /locus_tag="IWO17_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289830.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            8188..9480
                     /locus_tag="IWO17_000937"
     CDS             8188..9480
                     /locus_tag="IWO17_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0289831.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      9839..9936
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10052..10633
                     /locus_tag="IWO17_000938"
     CDS             10052..10633
                     /locus_tag="IWO17_000938"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ0289832.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            10636..11943
                     /locus_tag="IWO17_000939"
     CDS             10636..11943
                     /locus_tag="IWO17_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ0289833.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            12359..12847
                     /gene="purE"
                     /locus_tag="IWO17_000940"
     CDS             12359..12847
                     /gene="purE"
                     /locus_tag="IWO17_000940"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ0289834.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            12831..13964
                     /gene="purK"
                     /locus_tag="IWO17_000941"
     CDS             12831..13964
                     /gene="purK"
                     /locus_tag="IWO17_000941"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ0289835.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            13977..15272
                     /gene="purB"
                     /locus_tag="IWO17_000942"
     CDS             13977..15272
                     /gene="purB"
                     /locus_tag="IWO17_000942"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ0289836.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(15452..15829)
                     /locus_tag="IWO17_000943"
     CDS             complement(15452..15829)
                     /locus_tag="IWO17_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289837.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            16085..16813
                     /locus_tag="IWO17_000944"
     CDS             16085..16813
                     /locus_tag="IWO17_000944"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ0289838.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            16813..17067
                     /gene="purS"
                     /locus_tag="IWO17_000945"
     CDS             16813..17067
                     /gene="purS"
                     /locus_tag="IWO17_000945"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ0289839.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            17069..17740
                     /gene="purQ"
                     /locus_tag="IWO17_000946"
     CDS             17069..17740
                     /gene="purQ"
                     /locus_tag="IWO17_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ0289840.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            17741..19963
                     /gene="purL"
                     /locus_tag="IWO17_000947"
     CDS             17741..19963
                     /gene="purL"
                     /locus_tag="IWO17_000947"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ0289841.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            19948..21387
                     /locus_tag="IWO17_000948"
     CDS             19948..21387
                     /locus_tag="IWO17_000948"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ0289842.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            21419..22462
                     /locus_tag="IWO17_000949"
     CDS             21419..22462
                     /locus_tag="IWO17_000949"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ0289843.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            22459..23037
                     /gene="purN"
                     /locus_tag="IWO17_000950"
     CDS             22459..23037
                     /gene="purN"
                     /locus_tag="IWO17_000950"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ0289844.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            23034..24569
                     /gene="purH"
                     /locus_tag="IWO17_000951"
     CDS             23034..24569
                     /gene="purH"
                     /locus_tag="IWO17_000951"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ0289845.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            24585..25859
                     /gene="purD"
                     /locus_tag="IWO17_000952"
     CDS             24585..25859
                     /gene="purD"
                     /locus_tag="IWO17_000952"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ0289846.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            25981..26895
                     /locus_tag="IWO17_000953"
     CDS             25981..26895
                     /locus_tag="IWO17_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ0289847.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(27028..28593)
                     /locus_tag="IWO17_000954"
     CDS             complement(27028..28593)
                     /locus_tag="IWO17_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0289848.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(28586..30037)
                     /locus_tag="IWO17_000955"
     CDS             complement(28586..30037)
                     /locus_tag="IWO17_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0289849.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(30051..31067)
                     /locus_tag="IWO17_000956"
     CDS             complement(30051..31067)
                     /locus_tag="IWO17_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0289850.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            31287..32375
                     /locus_tag="IWO17_000957"
     CDS             31287..32375
                     /locus_tag="IWO17_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0289851.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            32429..>33719
                     /locus_tag="IWO17_000958"
     CDS             32429..>33719
                     /locus_tag="IWO17_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0289852.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFL"
BASE COUNT        10719 a         5755 c         7561 g         9684 t
ORIGIN      
        1 ttaaaattac atatttttcc atagaacttg catttgatcc aagatttcga tgacatcttt
       61 tgtcaagaag agagagcgat tgggcaagtc accttcaatc atgtgtacca tattctttat
      121 ttcgtaattc attgcttcag atgtattccc gctttcgatg aattcctttg taccatctgt
      181 aaataagatc tccgcacgat cagcacgcgg gtaatcagca atcgtcacat aaccgttctc
      241 aaaggcaacg ataccgactt ttggcatttt tgcctgaaaa gtcaagctga cagcagcaag
      301 ttcattttct ttgttacgca agatagtgac ggattgttcg tccacgcctg tttcaaaagg
      361 aaccattgta gaggcaacta cttcaggttg agagctcaaa aagaaacgag cgaaagatac
      421 agcatacgta ccgatatcca acagagctcc tccagctaat tctggattga agaaccggtt
      481 tttagggtct ggttctttgt aactgccgaa aggtgcttgg atcattttta aagcccccag
      541 ttttcctgta tccattattg agcgtaactg ctgataaagt ggcatgttga agattgtcat
      601 cgcttctgct aagatcaaat tcttttcttc tgctaagcgc atagcgtcag ccagttcttt
      661 tttattcatc gtgatggctt tttcacatag aacgtgtttg cctgcttcca atgctgccaa
      721 gatatggtcg atatgctgtc tatttggtac agcaatgtac acgatgtcga tttcagcatc
      781 gtctaacatt tcttgatagc ttccgtaagc ttttggaata ttgtaacggt gagcgaagtt
      841 ttccgctttt tctgaagttc tggaagcaac tgctgttagt tcgctcgtct cttgattgaa
      901 gctttcggca aactcatgtg cgatggtacc aagaccaacg attccccatt gatatttttt
      961 catctgaaaa cgctccttca tagttggatt gagtttcttg tctgtctatt attataacaa
     1021 aaatcacgaa gaataaagag gagaggactt gaaacattaa aaaaaatgaa agcagcgatt
     1081 taagaggtgc tttttgatca aaacttaagt agaatagtta aaaggagggg ataaacgtgc
     1141 atcaagtata tattgatctt attatgagtg tattattaga acagtttgaa actgaaaaaa
     1201 attttttaga tgaatacttg ctgatcagtc cagaacaatg gcaaaaatgg aaagatggcg
     1261 agaaaaatct gccatctgaa gcgatgcaga aagtaaaaaa tcttttcact gattatgaat
     1321 ggatgctaac gcaaaagatt ttgcgtcaaa cgattatttt tcccgaaaaa cgaaacgttg
     1381 ccgttgcaga gtacaagcaa atcaaaacac gcatcgcgca aaaatggctt agcagcaaag
     1441 cagcaaaagt cgagctgatc cctttgaaaa ataatcagca ggctggtaga taccttgact
     1501 tgaaagtaag tattcaatat gatgaatggg gctatgatga tattttgaat tttcgtctgc
     1561 cagcagatgt tcaaaaacag atcgaaggag aaaaaatcga acttctcgat tgggtgaatg
     1621 aaaatctgga agaaacttac gttcatgagg cgaatgggga acgtgaagaa caacgaaagg
     1681 atttttcaag tgaataaatt tttaggattt ggagcagttg tgattatgat cgcagcaatg
     1741 ggctatgtta tttttagcga tgatgattcg catgttcaag ccggcaatca agcagtcgaa
     1801 cgaacaagca ccaaaacaac tgctagtaaa gaaaagaaca gtcaaacaac cagcagcaag
     1861 gtaaaggaca aagaaacaaa agatcgtacg tccaaacaat tgccagatgt taaaacagac
     1921 gattggaagc tagttttggt tggtccagat cacaaattag aaaaagaaat tgatgagtct
     1981 actcaattgg catcattaag taacggatac ttgatcgaca aacgcattgc tccagaatat
     2041 gaagcatttg ccaaggctgc tgaagcagct ggatttccat tagtgatggt ctcagcttac
     2101 cggtctgtta tcatcccaac aacaagtttt cgaacaaaat gtccaagatg tgatgagtcg
     2161 tcaaggtgta tcagaagagg aggcgacaaa gattactaaa gaaacgatta cggagccagg
     2221 atacagcgaa caccacacag gtctggcagt cgatgtcgtc gatcaaaact ggtacaacag
     2281 ttatccttct actgtattgg atgcaagtta cggagatcag cctggagcta aatggatcgc
     2341 agataatgct gcgcagtatg gatttatcgt tcgttatcca aaaggacacg aagatataac
     2401 taagatcaca tatgaacctt ggcacttacg ttatgtcggt aaagaaaacg ctgagtatat
     2461 caccaaacac caattgacgc tagaagaata tctgaatgaa ttaaatgaaa agtaggaatt
     2521 gatcatggcg aaaaaaaagt ggaaaaagca aaagcgcaag aatatccgtt ggccagcagt
     2581 ggttgccggt gtagcagtga tactcttagc ctttgttttt tccttgaaga atctgtcttc
     2641 tccttttact gataatcagc aagaaaatga agcgcaatcc catcagcaat ttattgaacg
     2701 gcttgttccg catgcccaag aactacaaga tggttatgga gtgcttccta gtatcatttt
     2761 aggacaagct atattagaat caaattgggg aaaaagtacc ttggctagca aatacaataa
     2821 tcttttcggg atcaaggctt atggggatca gaagaaagta agcctggaaa caaaggaatt
     2881 tgttaacgaa gaatggataa ctatccaagg agacttcaaa gtgtatgatt catgggaaca
     2941 gtccatggat gaccatacac aactgttcgt ccaaggagta gattggaatc ctgcgctgta
     3001 tgaaaaagtg attacggcca ccaactatca ggaagccgca caagcattgc aagatgcagg
     3061 ttatgcgaca gaccccggtt atgcgcaaaa aatcatccag gtcatcgaga cgtatcaatt
     3121 gaatcagtat gaccggagat agcaaaggag aagtggctga tggcagaaca atttacgcca
     3181 gaaatcatga cggaattgag aaaagatatc gtaagagtac cggaaatcat tttagaagca
     3241 agcgggatca agatatttgg aaaattgatc cgttcgatca tctttactac ggatattgcg
     3301 atcatacgta atacaaatgc gaatgcagta attgctgtgt atccttttac acctcatcca
     3361 gcgattacta aaagcattat tgaggcagct gacattccag ttttctccgg tgtaggtggt
     3421 ggtttgacac aaggtgctcg ttcttcctac atgagcatgt tcgcagaagc gcaaggttcg
     3481 ttaggggtcg ttttgaacgg tcctacgcct ttagagacag taaaagaagt ttgtcgagta
     3541 gttgatattc cagttatcag tacggtaacg tcgaaatata cagaaataga tgaaaagtta
     3601 gcagctggta tttcgattat caatatcagt gcaggtaaag aaactgcaga agcagtacgc
     3661 cattttcgta aaaaatatcc tgaacttcct atcattgcga caggtggtcc aacggaagaa
     3721 agtattgctg agaccattca agcaggagca aatgccatca catatacgcc accaagtagc
     3781 ggagagcttt ttagtcgtaa aatggaaaaa tatcgggaaa ctgagaaaaa gaatcatgaa
     3841 taacaggtga gaatcaaaag acgaggaaga gcagatagtg gctcttcctc gtcttttgta
     3901 ctccagtact atttgttcag aattcaaaaa aaagagaaat ttttttgttt ttttacctgt
     3961 aatgtacata agaagagtta aaaccttttg ttattattag cattctaaca aaaaaatgtc
     4021 ttgatttatt gaagcgcttt ctatagaatg aaaaacgaat aaaaactaaa aaaggcaccc
     4081 gacaatttag tagcagctaa attgtcgggg cttgaaaagt cagcgggaac tgaccaatca
     4141 agttgccata gtcaatgctt gtgcatcgac gtgtatattg tacctaattt catacagaat
     4201 tactagttat ttttctgttt tttagggaag agcgcacacg aagaagcatg cttatttagc
     4261 tatgtcaagg atattgggca gttgtctgat atcctttttt ctttgcgtaa ttatcctccc
     4321 atcattgaag caagaagtgt ttttagcgat gaaagaagaa tcagcactat ctaatttttc
     4381 ttgaaagtat tgagttgttc atcctaggca gaacgataag gcaagtcttg gtttttattc
     4441 cccttgaaaa agatcgttta ataaatatgt tctgtataaa aatacagtat attgaaaaat
     4501 aacaatggaa gtagaggtgc agagaatgaa gaaaagaatg agaatcatct tagctttaag
     4561 tgttatgtta ttcttcttga cggcttgtgg gcaagaagat gacaaaaaaa tcacgctgaa
     4621 catggcttgg tggggttcgc aagtccgaca cgatgcaaca gtaaaagtaa tcgagcttta
     4681 tgaagaaaaa aatccccatg tgacgatcga ttatgaattt tatgattatg aaggatatta
     4741 cacaaaatta ggtacactag cagcatcaga tgatatctgg gacattttcc aactgaacaa
     4801 tcaatttcct caatacatca atcagattga accattagat gaatatgtgg agcaagggat
     4861 aattgatatt tctaatgcag atgaatccta tttagcaacg acgacttacg acgggcaatt
     4921 agttggactg tctaacgggg tgaattcgtt tgcgattgct tacaataaaa atatatttga
     4981 acaattaaat atccctgaac cagcttcaaa ttggacatgg gatgaatttg aacaaatcag
     5041 ctatcaaatc actgaagaat taggaatcta tgctatttca agattcgaag attttatagc
     5101 tggctgtatt attcaaatac cacaagttga gaaaggattg aatttttata atcgggaaga
     5161 tctatctaac agcttgggat ttgaaaatcc agactatcta acagattttt tccagctgcg
     5221 taaaaatctg gtagacaaag gggcccatcc agaaccaggt ggtggagctg caagtacgac
     5281 ggatctgtct tttcaggcgg ttagggatag agaagcggca atggtgtttc tatcaagtaa
     5341 ccagttgaca gaaatattgt caggggctcc tgaagatatg gagattcagt tgattaatcc
     5401 acctcgcaga aaagcagatg gagaatctgg tatcccttta cgttcttcgc aaatgctgag
     5461 tatggcaaaa agctcagccc ataaagaaga agctgctaag tttattgatt tctttcaaaa
     5521 cagtgaagaa gccaatgaaa ttttaaaagg agaacgtggt gtaccaatca tgtcgaatat
     5581 tcgagaacag ctcgctcgtg cagatgatgc aactttgcaa gcgacatttg attatttaga
     5641 aatgatttcc gatatggaca acggcgaagt gaatgtcttt gatagtgctt tgaatccgga
     5701 aattcaagac caatacaatc ttttagttga aaaagtcatt tatgaagaag taacagcaga
     5761 agaagcagca gagagtcttt atgagtttgc gtcaaacatt ctaggatagc agcaggagga
     5821 aggcatgaaa aaagaaaaac taaaacggtt cttggatcaa gacaatgtgg tcggttacgt
     5881 atttgcttct ccattcatcc taggatttct attgataaca gtgataccga tgatcttgtc
     5941 actttattat tcgttcaccg attatcgttt gggaaatcca gtctcatgga tcggcttgga
     6001 taattatata aagatgctga gtgatgcccg ttttttgaac tccatccgag tcacttttct
     6061 ctatgtattt gcatctgtac ctgtgaaact gatctttggt ctattgattg cttatctttt
     6121 aacgaggaat atcaaaggaa cgactttctt tcgttctttg tattatgtgc ctagtttgat
     6181 cggtggcagt atttctatcg ctctagtttg gcgggagttg ttttctaaaa acgggttagt
     6241 gaatattttc ctggaacgat taggcgcacc tgcgctttcc tggtttagcg atcaaaaact
     6301 ggtcatgatc cctcttgttt tgatgtctgc ttggcaattc ggttcggcaa tgatcatctt
     6361 tgctgcaggt atcaaagaga tcccaggctc gtattatgaa gcggcagaga tcgatggagc
     6421 gaacaaatgg cagacgtttt acaagatcac gttacctctt ctttcgccga tcattttgta
     6481 taacttggtt atgcagacaa tcgcggcgtt tatgactttt acgcaggcat ttatcattac
     6541 ccaaggtggt ccgaatgatg ccacgaactt ttatgctctt tatgtgtata accaagcttt
     6601 ttcttttaat tctatggggt atgctagtgc tatttcgtgg atcatgctgt tgattatggg
     6661 agctgtcacc tttctgattt tcaaatcatc aaaaaaatgg gtcgttgtgt gaatgagagg
     6721 aggaagaaat gaataataca gtaacgaaat ggtttcaata cggttttatt ttgctattag
     6781 gtctagtcat gatttatccg gtcttatgga tgatcgcagg ttcttttaaa acggatcaag
     6841 agattatgag tggttcgctg aatttgattc cagagacttt tcaatgggaa aattacgcaa
     6901 aaggatgggc ggggtttgct ggaatttcat tttttacttt ctttaggaat tcgtttgtca
     6961 tctctgcagt ttcgacgata gggacagttc tgagctcgac ttgtattgct tacgcattat
     7021 cgagaatcga tttcaaagga aaaagattct ggttcgtcgt catgttagta acgatgatga
     7081 ttcctgcaca agtgattttg attccgcagt ttattattta caatcggctg aatttggttg
     7141 ggacgtatgt gccgctgatt ttaccacatt tttttggaca agcatttttc atttaccaga
     7201 tcatgcaatt catggtcagc atccccaaag agttagatga atcagcgatt attgacggat
     7261 gcagcaagta tagtgtcttt accaaaatta tttttccttt gctgaaacct tcgatcatca
     7321 cgacgatcat catccagttt tattggaaat gggacgactt catgggacca ttgatttatt
     7381 taaacaagcc gagatcttac acggtatcga ttgccatcaa attatttgca gacgctggat
     7441 ctagtacgac ttacgcgagt atgtttgcga tgtctacgtt gtcgctgcta ccggtttttc
     7501 tgattttctt attcttcaat aaatatcttg tccaggggat cagtacaagc ggtctgaagg
     7561 gctgaatatg aaaaagccaa tagaagagat gtttctttac aagctcaact actatgtctg
     7621 tcggattttt ttaaccagtc ttatcagtaa tgctcttttc gctttagttc taggatgttt
     7681 gttcacttta ggattagtgc tgtctgctcg tgttgtctat ctgatttatt ttttgatgat
     7741 gtatccttct tattatctat ctgtccatta cttggcagaa aaaaaggtat gttggaggaa
     7801 aggaaaaatc aaacggatgt gtttcatcat tacgttgttt cagctgtttg tcattatttt
     7861 tgcagttgat tggtatttat tagtgggaaa gattggagct tggtatctga ttccttttct
     7921 attgtgtctg ttgctttttg tatcccatag tttttttagt acgctaattg atatctgcaa
     7981 agaagataaa gcagatagtc ttgtttattt gatcaaaagg aatatctggc tgttgtcaaa
     8041 aaacgtcaag gggtttctgt ggtatttatg ccttttgtat ggactgttct ggtgtataca
     8101 gcatgatttt ttactcactg cattactgta tccagggctg ccatttgtca cagtattttg
     8161 cttaaaagaa aaggagaatg actttaaatg aagaaaaaat atgtacaagt cggacttggt
     8221 gggagagcgc gtttttttta tgaggcaatt gcctctacgt atcatcaaac ttgtgaacta
     8281 gtcggttttt gtgatgtatc tcaaacaagg atggattatg caaatcggat acttgtgaac
     8341 aagtatggac agaaaaaagt aaagacatgt atgccatcac aatttgagca actactggat
     8401 gacgtacggc cggctgaagt cattattacg tcagtagatc gaacgcatca tgattatatc
     8461 attcgtgcaa tgaaaaaagg atatgatgtt atttgcgaaa aacctctgac tatcgatgag
     8521 gaaaaagctc aagaaataat aacggtccaa aaacagacgg gaagaagcct gcgagtcact
     8581 tttaattatc gttatgcacc gtatcacacg aagataaagg aactgataga aagtgatgtg
     8641 attggtgatg tgttttctat ccacttcgaa tggctgttaa atacgcaaca cggggcagat
     8701 tatttcagaa gatggcatcg aaataagcac aatagcggcg ggcttcttgt tcataaatct
     8761 acccatcatt ttgatctagt gaatttttgg ttgaacagcc gtccccaaaa ggtatttgca
     8821 ttcggtggat taaactttta tgggatgcat aatgcggaga aacgcggagt agaaatattt
     8881 tacagtcgat gctatggaag cgaactagct aaagctgatc catttgctat agacctgtca
     8941 gccaatgagg aattgcgaga aatgtattta gaagcagaaa aagacagtgg atatattcgt
     9001 gaccgcagtg tattttccga tgatatctct attgaagata cactaggcgt aacggtcaaa
     9061 tatgaaactg gtgcgatttt gacgtattcg ctaaacgcgt atatgccgtg ggaaggattc
     9121 aatgtttctt tcaatggcag caaaggacgg atcgaaatga aagtgatcga aaagtcttac
     9181 atcaatgcag gtggagataa aaaggatgaa ggagcagccg ttcttcagtc catccatgtc
     9241 tatccgatgt tcggtgagcc atatgaggta atcgtcgaac aagaagcggg cggacatggc
     9301 ggaggagatc cgcgtttatt agatgatcta tttggtgaag aaaaagaaga agatccgtgg
     9361 aatcgggcag caactcacgt agatggtatt ttgtcgattt tgacaggaat cgcagcaaat
     9421 cattctattg cttcaggaaa agcagtagtg attgacgagc ttgtcagctt aaaagaataa
     9481 gaatagattt acggaaatta cttcccgtag tttataaaat cttgccttat tgccgatagg
     9541 ctgtcttttg aacactattt attcggaaaa aggggctatg acatgctttt tgtcatgcct
     9601 tctttttatg tgggctgctt ttttagatag gagaaaataa aaataactat ttaaacccga
     9661 acaaaaagga ataatgcatt aaaaatcatt agattttaca aaaaaataag taaaccacgt
     9721 tgtcagtttc agaaagtagt gttagactgt tttcaatatt tgaaacaaca ttttttattc
     9781 aaatatggca aactgccttc acgtgcaaaa cagttgcaag aaaatgaatt tacactatta
     9841 ttagttttac atacctactt atatatcgtc ataatatgga tgacagtttc tagccagtac
     9901 cgtaaatgct ggactataag taaaagattg gctatttcac ccttcttttc ttatgataaa
     9961 gtggagggtg tttttcttta acagaaacat agtaggtttt tgcacaaaag cggttatttc
    10021 ggtattaata aatgataaag gagcaaataa agtgaaagaa ttagttgaac gtattcaaaa
    10081 agacggacgt atattaggcg agggcgtatt aaaagtcgac agcttcgtca ctcaccaagt
    10141 agaccctgta ttgatggaac aaatgggaaa acggtttgca gaagtattta aagaacaagg
    10201 aatcacgaaa gttgtgacca ttgaagcttc tgggatcgca ccagctttat ttgctgcaag
    10261 agaattagat gttccaatga tcttcgctag aaaagcaaaa agtttgacga tggatgaaga
    10321 attattgaca gcttctgttt attcttttac gaaacaagtg acaagtacga tttctatttc
    10381 tcgtaaattt ttatcttcag aagacaaagt attgattatc gacgatttct tggcaaacgg
    10441 acaagcggca aaaggattga tcgaattatg ccagcaagct ggagcgcaag tagaaggaat
    10501 cgggatcgtc atcgaaaagt ctttccaaga tggacgtcag ttacttgaag aaatgggatt
    10561 gagagtcgtt tctcttgcgc gtattgcttc cttgacgaat ggacaagttg agtttcttga
    10621 ggaggacgca taatcgtgga aaaagaaaca caaaatggaa aagctgcggt tttagggctg
    10681 cagcatctgc tagcgatgta tgcaggagca gtagcggtgc ctcttttgat cggtacagga
    10741 ctaggcttta atcaagaaca gatgacttat ttgatttcaa ttgatatctt tatgtgtgga
    10801 ttagcgacat tattgcaatt gacagttaac cgatttttcg gaattggctt gcctgtcgtt
    10861 ttaggatgtg cgatccaagc agtagctcca ctgatcttga tcggaaccaa tgaaggagta
    10921 ggagctatct acggatctat tatcgcttct gggatctttg ttgtgttagt ttctggtgtc
    10981 ttttctaaaa ttaaaaaact attccctcct atagtaacag gaacggttat caccgttatt
    11041 ggtttgactt tgattcctgt agcaatcgag aaaatgggtg gaggtaatgc aacagcagct
    11101 gattttggtg acaaaacaaa tctgctactt gcatttgtga cgattctcct aattgtaggc
    11161 gtgcaaatgc tggcaaaagg gttcattcgt tcaatcgcgg tattgattgg tttagtcgga
    11221 ggaagcatct tagctgcttt catgggaatg attgatctga gcgcgattgg agatgcgccg
    11281 ttgttccatg ttccacaacc cttttatttt ggtaaaccaa ctttcgacgt atggtcgatt
    11341 ttactaatga tcatcatttc catggtcagc atggtagaat caacaggtgt atattttgca
    11401 cttggtgaca ttactgggaa aaatgttgga gaagaagaat tgaaaaaggg atatcgtgca
    11461 gaaggacttg ccgttatctt aggcggtatt ttcaatacat tcccttatac tggcttttct
    11521 caaaatgtcg gattggttca actttcggga atcaaaacaa gaaaacccat ctatttctct
    11581 gcattcttct tgattgtttt aggattattc cctaaaatcg gtgcgttagc acaaatcatc
    11641 cctgagccgg tacttggtgg aggaatgtta gtcatgttcg ggatggtggc agtccaagga
    11701 atccgtatgc tgttggaagt tgatttcagc aatgataaaa atctattgat tgttgctgtt
    11761 tctgttggat ttgggctggg tttcaatgtt acgccgacat tattcagtca aatgccgcaa
    11821 acattgcaaa tgttcactgg aaatggcatc gtaatgagta gcattacggc tatcatattg
    11881 aatttgatct ttaatggatt gaaaaaagat gaagaaatat tggaagaagc cttcaagaag
    11941 taatagagca aaagaaaggt tgtgaaatca gcgttttgac ctgattgtta gagaaacaaa
    12001 cggaaaaata gttcctcgta ttttgccgtt tgtttgagct aataccgagg gtcagctgat
    12061 tgaacaccgt tgaaggaaga ggtcgtgagc ctgccgttca tctccgagtc acaaggaaca
    12121 atttcaaaaa aaagcttctc atgtttgttg aatattgttc cttgtgaccg aggagttggc
    12181 aggagaacac cgtttatcca taaaaaggaa tgtgacctaa cttttgtcac attccttttt
    12241 tttatcttta ataaacacga acaataaaat gtttttcttt gttaacattc gaaatttctt
    12301 tgacatcaaa aaaagtgatt gttaaaatat gaatgtagaa aaactgggag gaaaaacaat
    12361 gacagcaaaa atctctgtga ttatggggag tacttcagat tgggaaacaa tgaaagaagc
    12421 atgtcaaata ttggatgaat tcggtgtgtc ttatgaaaaa aaggtcgttt ctgctcatcg
    12481 aaccccagat ttgatgtttg aatatgcaca aaacgctcga aaaaaaggaa tcaaaatcat
    12541 tattgcagga gcagggggag ccgcgcatct gccgggtatg gtcgcatcaa aaacaacttt
    12601 gccggttatc ggtgtaccgg tccaatcacg aacattaaat ggtttggatt cgcttctttc
    12661 gattgtccaa atgcctggag gagtacctgt agctactaca gcaatcggaa aagcgggagc
    12721 aattaatgct ggactacttg cgatacagat gctttccatg tatgatttag aattggaggc
    12781 taaattggct gaaagacgtt caatgttggc taaaactgtt atagaaagta gtgatcaact
    12841 tggttaaacc tttgttgcct ggttcaacga tcgggatcgt cggtggcggt caattaggtc
    12901 gtatgatggc gattagtgca aaagaaatgg gctttaaagt aggtgtgctg gatcctgtga
    12961 gcgattgtcc agccgcacaa gttgcagatt ggcatattga aagtgtctat gatgacacct
    13021 ttgcgctgaa agagcttgcc agaagaacag atgtcattac ctatgaattc gaaaacgtca
    13081 gtgtagaggc gttaaatgcg attttaccca tgtcatttat tcctcaaggg acagacttgc
    13141 ttgcgattac gcaagaccgc ctgttagaaa aatctttttt ggaaacgaat aacatcgtga
    13201 ttgctcctta tgcaacgatc gtcagtccga cggatatcca agatgcgatt gacgggatcg
    13261 gctatccttg tgttttgaaa accactcgag gcggctatga tggcaagggg cagtatgttt
    13321 tgaaaagtcg ggcggatcta gctcctgcca tggacctttt aagagaagga acctgtgttt
    13381 tagaagcatg gatcccgttt gaaaaagaga tttcgatcat ggtggcagga aatggtcaag
    13441 gagatttcac gacttttccg gtcgtggaaa acatccacca caacaatatt cttcatgaaa
    13501 cgattgctcc agccgcgatt gatcaggatg tgatcgagga ggctgagcgt attgcccgtg
    13561 tgattgcaga agcagtgagc ctttcaggtg ttttaggggt agaaatgttt ttaacaaaaa
    13621 caggcgggct atatgtgaat gaattagctc ctcgtcctca caattctgga cattattcta
    13681 ttgaagcttg ttctatgagt cagttcgatg cccatattcg aggtatttgt ggttggccat
    13741 taggagaaaa agtacgtttg ataagcaatg cagtaatggt caatgttttg ggagaacaac
    13801 tgctggaaag ctatcgcttg attgagaata agccagactg gcaattccat ttctatggaa
    13861 aaaaacaagc aaaaaaaggt cgcaagatgg gacatatcac tatcttgaca gaagacatcc
    13921 agcaaacgct ggaagaaatc aaacaaacga atatttggga ttagaggaga aaaaacatgt
    13981 tagaacgtta tacacgccct gaaatgggga atatctggac ggaagagaac aaataccgtg
    14041 cgtggctcga agtcgagatt ttagcagatg aagcttgggc agaacttggt gaaatcccgc
    14101 aagaagatgt gaaaaaaatt cgtgagcatg catcatttga tatcgatcgg atcttggaaa
    14161 tcgaaaaatt aacacgccat gatgtcgttg cgtttacacg agcggtttct gaaacattgg
    14221 gagaagaaag aaaatgggtc cattatggtc taacaagtac ggatgtcgta gatactgctt
    14281 atggctatct catcaagcaa gcaaatgata ttttacggga agacttgaaa cgttttacgg
    14341 aaatcattgg cgaaaaagca aaagaacaca aatatacagt gatgatgggt cggacacacg
    14401 gtgtccatgc tgaaccaact acttttggat tgaaacttgc cacatggtat tcagaaatga
    14461 aacggaacat cgaacgattt gaacatgcgg caaaaggagt agaagcagga aaaatctcag
    14521 gagcagtggg aacgttcgca aatatcccgc catttattga agagtatgta tgtaaacaat
    14581 tggggatccg tccacaagaa atttctactc aagttctccc tcgggatcta catgctgaat
    14641 atttctcagc gatggcgctt attgctacaa gtattgaacg ttttgcaaca gagatacgcg
    14701 gattgcaaaa atccgaaaca cgtgaagtag aagaattttt tgcgaaaggt caaaaaggtt
    14761 cttctgctat gccgcacaaa aggaatccga tcggttcaga aaatatggct ggtctagcca
    14821 gagtggtgcg cggacacatg gtcactgcct atgaaaatgt cacattatgg catgaaagag
    14881 atatttctca ttcttcagca gaacgaatca tcttgcctga taccacgatt ttattgaatt
    14941 acatgttaaa tcgttttggc aatatcgtta aaaatctaac ggtctttcct gaaaacatga
    15001 agcggaatat gtctgcaaca tttggtttga tttatagcca gcgcgttatg ctgaaattga
    15061 tcgataaggg aatgacaaga gaagaagctt atgatcttgt tcagccaaaa actgcgtacg
    15121 cttgggacaa ccaagtgcag ttccgcccgc tgttagaaga agatccaacc atcacatcgg
    15181 tattatctaa agaagaaata gacgatgcct ttgattatca tcatcatttg aaaaacgttg
    15241 acgtgatatt caaacgagta ggtttggaat aaagaagagt gagttgcaaa attcaattaa
    15301 aatagaatag caaaaataac caaaaaagaa gcagatccgt tagcggttct gcttctttta
    15361 gtttattata ttttcttatc aataatagga acctgatttt aaaagcggct acctctttac
    15421 cgtgcctctt tacatacaaa aaatagagga attaagcgta aatggcttgg ctattataac
    15481 taaaatgctg gttatggaga tgttgcagca agttttcgag aaaagcgtat tccatctctt
    15541 gtccattgaa taagatctgg atacgataat gatttttgaa acgcttctgt tcatgaatct
    15601 gactgattga aagttcgcct ttgtgttctg tctgataaag aaaagtcctt ttacggcatt
    15661 cagcacagcg cttggttgct cggcggattt ttcggaatgt ataaaaacta gtatctttat
    15721 tgcataaata aacgtctttg atcgacagca aatcattata tagacaggaa cgttcttcag
    15781 ctgttaattc tgataaaaaa agctgacctt gttttattgc atcttccatc ggcaagtgcc
    15841 tcctcgaacc cattatccaa atttttagtt aattctaata taccatcatt aagtataaat
    15901 gtaaacgctt gttgatggta taagattttc gtttctgata aaacacgaac attaaaagaa
    15961 tctaatgcct taaatgtaca tatttttgtt gatttataaa aagatgattg ctaaaattta
    16021 aagtgaaaaa acgaacaaaa aacgatgtat ttgcttttaa tgtaaataaa aaggagagac
    16081 gtacatggag aagaaagaac ttttgtatga aggaaaagcc aaaaaaatat atcttacttc
    16141 tgatgagaac ttgatttgga tagaatattt agatcaagca actgcgttga atggcgcgag
    16201 gaaagaccag ataagtggaa aaggagaatt gaataatcaa atcacaagtg caattttccg
    16261 ttatctgact caaagaggaa taaaaaatca ttttattgaa caattgtcta aaaccgaaca
    16321 attagcaaaa aaagtacaaa ttatccctct ggaagtggtt atccgaaata ttgcagcagg
    16381 aagtttctca aaacgtctag gtgttcccga aggtaaagaa ttcatcaaac caatcattga
    16441 attctactat aaggatgacc agttggatga tccatttatc aatgaagagc atatctattt
    16501 cttacaactt gcaaagccgg aagaaatcag tgagctgaaa gcccaagcgt tggctattaa
    16561 tgaaattttg accgaattat ttgctaaggt cggtttggtt ttagtagatt tcaagctaga
    16621 atttggccgg tcgaatggac aaattttgct tgcggatgaa gtaacaccgg atacttgccg
    16681 attatgggat gaacgtacga atgaacatat ggacaaagac gtttaccgca gagatctagg
    16741 tgatttgatc ccagtctatc aagaagtata taaccgtcta tcggaactaa ctgaagaaaa
    16801 ggagcgagta tgatgtattt cgtaaaagta tatgtaacct ataaagattc agtgttagat
    16861 cctcaagggg aggcagtaaa aaaagcagtc catcgtatgg gatatgaaac aatcgaagat
    16921 atccgaatag gaaaatactt tgagatacaa cttgctgatg cagaagaacc aatcgataca
    16981 gtgattgaaa cgatttgtga caagctgctt gcaaatgtaa atatggaaac ataccgatat
    17041 gaaatcaaaa aaatggagga agtataacat gaaatttgcg gtcatcgttt ttccaggttc
    17101 aaattgtgac gcagatatgt tatgggcagt tcgcgagatc atgggcgctg atgccgaatt
    17161 tgtacggcat gatgagtcct ctttagaagg atttgatggg gttttactgc cgggaggatt
    17221 ctcctatggt gactatttga gatgcggtgc gattgctcgg ttttctacaa tcatggaaga
    17281 agtgattcgt tttgctgaag aagggaaacc agtattcggt acatgcaatg gattccagat
    17341 attgacagaa gcaggacttt tgccaggtgt actgagacga aatgagtctt tgcattttat
    17401 ttgcaaaact gtaccattga aagtcaaccc caagacggcc tttacttcag agtatgaagc
    17461 cgacgaaata atctatcttc cagttgctca tggggaagga aattatttct gtgacgaaga
    17521 aacacttcaa gaactaaaaa aaaataatca gattgccttt acttataatg acaacatcaa
    17581 cggcagcgtg gaaaacattg cagggattac gaacaaagca gggaatgtgt taggaatgat
    17641 gcctcatcca gaacgtgcag tagaagactt gctaggatca gaagatggga aacggttctt
    17701 tgcttccatt ttaaaaaact tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc
    17761 gacagctgaa gagatcaaga aaaaaagaat ctatgccgaa tggggattga ctgatgaaga
    17821 atatcgaatg atcgaagaag atatcttagg aagaatgcct aattatacgg aaactggact
    17881 gttttctgtt atgtggagcg aacattgttc ctataaaaat tctaaaccgg ttctacgcaa
    17941 atttcctact agtggacctc atgtgttgca aggccctgga gaaggcgcag gcatcgtcga
    18001 catcggtgat ggactagcag ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt
    18061 ggagccttat gaaggagctg cgacaggagt tggcggaatc atcagagata ttttcagcat
    18121 gggggctcgt ccaattgcgt tgctagattc attgaggttc ggcgaattag ataatccaag
    18181 aacgaaatat ttactagaag aagtagttgc aggtatcggc ggatacggca attgtatcgg
    18241 cataccgact gtaggcggcg aaattgcatt tgatccttgt tatgaaggaa acccattagt
    18301 caatgcaatg tgcgtaggtt tgattgaaca caaacatatc cagaaagggc aagccgctgg
    18361 cgtcggtaat tcgatcatgt atgtaggtgc aaaaacaggt cgcgacggta tccacggagc
    18421 aacttttgcc tcagaagaat ttaatcaaga agaagaacag caacgttctg ctgtacaagt
    18481 aggcgatcct tttatggaaa agctgctgct tgaagcgtgt ttggaattaa tcttgcatca
    18541 ttcggattct ctaataggta tacaggatat gggagcagca ggacttgttt catctagtgc
    18601 ggaaatggct tcaaaagctg gctcaggact tatattgaca ttagatgaag tcccacaaag
    18661 agaaactggg atgacacctt atgaaatgat gttatcggaa tcacaagaac ggatgttgat
    18721 ttgtgttaaa tcaggagagg aagaaaaaat ccaagaacta tttcagaagt atgatctaga
    18781 tgcagtcact atcggtaaag taacagacga cgggcaatat cggctatatc atcaaggaga
    18841 agaagtagca aatctgccag tcgatgcatt agctgaagat gcaccagttt atcataaaga
    18901 aatgaaagaa ccagctcgaa tcgtagaatt tcagcagtta gctccttatc agccagttat
    18961 cgaagaacct ggacaagtat tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg
    19021 ttcaatctac gaaacttatg attcacaagt acaaaccaat acagttgtcc gtccaggaag
    19081 cgatgctgct gtgatgaggg ttagagggac gaataaagcg ttagctatga ccacagactg
    19141 caacgcgcgc tatctgtatt tggatccaaa aatcggagga caaatagcag tggcagaagc
    19201 tgctagaaat attattgcta gcggtggtaa gcccttggcg atcaccgact gcctgaacta
    19261 tggttctcct gataaaccag aagttttttg ggaattatcg acttcagctg acggaattgc
    19321 tgctgcgtgt gaaacgttag gaactcctgt tatttcagga aatgtctctt tatataatga
    19381 aacggatggt caagcaatct atccaacacc aatgattggt atggtcggat tgattgagga
    19441 tcataaacat atcactactc aggaattcaa aaaatcaggg gatctaatct atatattagg
    19501 taagacgttt gcagattttg atggcagtga attacaaaag atgcagcttg gaagaatcga
    19561 aggtgtgatc cgaaactttg atctatccat tgaaaagaga aaccaagagt tagtattgac
    19621 agccatccaa aacggattga ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat
    19681 tgcactggca gaatcagctt tcaaacatca gctagggata tctgtacagt ttgaattgtc
    19741 ctctgctcaa ttatttgcag aaactcaatc aagatttgtg ttgacagtcg caccggaaaa
    19801 taagacacga ttcgaagaaa tgatgggaga tgctgctgtt ttagcaggta aggtgacgga
    19861 tgaagcgata atcgaaattt cagcaactga cggtcagatc aaaatagaaa ctgcagtagc
    19921 aagaaagtgc tgggaggatg caatcgtatg tcttatgaag tgaagagttt aaatgaagaa
    19981 tgcgggatct tcggtgtttg gggtcatcca gacgccgcta gtgtggcata cttcgggctc
    20041 catagtcttc agcatcgagg acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag
    20101 cttaaggggc atcgagattt ggggctgctt tctgaggtat tcaaagatca aagaaaattg
    20161 cagcagttaa caggagaagc tgcaatcgga catgtacgtt atgcaacagc aggcaatgga
    20221 agtgtggaca atattcagcc ttttctattc aaatttcatg atcagtcgat cggcttagcg
    20281 cataatggta acttgatcaa tgctcgttcc cttagaaaag agttagagga aaatggggcg
    20341 atcttccatt ctaactccga tacggagata ttgatgcatt tgatccgacg aagtgaagaa
    20401 acagattttt taaataaatt aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat
    20461 ttattaatga cagaaacggc aatgattgcc gctttggacc caaatggttt tcgtccatta
    20521 gcaatcggac aaatgaaaaa cggagcatac gtgattgcct cggaaacttg tgcattagaa
    20581 gttattgggg cacgttttat aagagatgtc cagcctggtg agatagtgat catcaatgac
    20641 gaaggaatcc agattgattg ctttacgaag gacacgcagc tttctatttg ttcgatggaa
    20701 tatatctatt ttgccagacc agattcaaac atcgcaggga tcaatgtgca tactgcacgg
    20761 aaaaatatgg gacggaattt agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc
    20821 gtacctaatt cttctttgtc agccgcaagc ggttatgcgg aagctagtgg aatcccttat
    20881 gaaatcggat tagtaaaaaa tcaatacatt gcccgaacat ttatccagcc aacgcctgag
    20941 ctaagagagc aaggtgttcg aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa
    21001 aaggtgatta tggtcgatga ttcaattgtt cggggtacga caagcagacg gatcgtccat
    21061 cttttgaaag aagcaggggc aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa
    21121 tatccatgtt tttacgggat tgatattcaa acgcgtaaag aactcattgc ggcaaatcac
    21181 acgattgatg aaatcaaaga atgtatcggt gcagattctt taggtttttt aagtgaagag
    21241 ggtctgatcg aatcgatcgg tctggaaaaa gatgctccgt attcaggatt gtgtatggct
    21301 tatttcaacg gagattatcc gacgccgtta tatgactatg aagaaaaata tcaggaatca
    21361 ctaaaagaaa aagtatcgtt cttttaaaag cagaccaaga agggagtata tgaattcaat
    21421 ggcaaatgca tacgcaaaag ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg
    21481 gatcaagaag catgtcaaaa aaacggaacg tttaggtatg atgggcgcta taggaggatt
    21541 cggcggttgt tttgatttat cggtctttca attaaaagag ccagtccttg tctcggggac
    21601 cgatggcgtg gggacgaaat tagcagtggc aatcgaagca gacaaacacg atacaatcgg
    21661 gatcgactgc gtagcgatgt gtgtcaatga tatcttagca caaggcgcag agccgttata
    21721 tttcttagac tacttagcaa cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa
    21781 aggtgtagca gatggatgtg ttcaaggaaa tatggcgctg attggcggag aaacagctga
    21841 aatgcctggt atgtactcag gtaatgatta tgatctagcc ggatttgctg tagggatagc
    21901 tgaaaaatct caattattga caggagaaac gatcaaccca ggagatgtgt tgcttggaat
    21961 agcatctagc gggattcatt caaatgggta ctctcttgtt cgcaaaattt tctttgagca
    22021 gcagcagttt tctttatcat caaaactgcc agaattgaaa aaaacacttg gcgaagaatt
    22081 gcttgagcca acgaaaatct atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat
    22141 aaaaggagct gcacatatca ctggtggagg gtttgtagaa aatatcccgc gaatgttacc
    22201 agaatctatg gctgcacaaa tcaaacttgg ctcatggccg attcttccaa tattcggctg
    22261 tttacaaaaa tatggtgaaa ttcctgagat ggagatgtat gaaatcttca atatgggcat
    22321 cggtatggtc ctggcagtcg aagaatcgaa agtaaaagaa atagtacacg aaattgagca
    22381 agctggcgaa aaagtttatg aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt
    22441 gtttatagga gaagaacgat gagaatcgct gtttttgctt ctggaaacgg aagcaacttt
    22501 caagcgctag ccgattatct atcgaaaaaa ggattggaat cttcgattga ctggttgttt
    22561 tgtgatcagc ctgaagcata tgttttgaaa cgtgcaactg cgttgagtgt accggcagat
    22621 tgtttttcac ctaaagaatt tgattcaaaa aaagagtatg aagaagcaat tttgcataaa
    22681 ttgaaagaaa aaaagatcga tttgatcgta ttagctggat atatgcgaat cattggaccg
    22741 gtattgctga aaaattatga taaaagaatc atcaatatcc atccgtctct tttacctgcc
    22801 ttccctggtt tgcatggcat acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc
    22861 acaattcatt atatcgacca aggtgtagat actggaccaa tcatccggca ggaaaaagtg
    22921 agaattgaac aggaggacac gttcgattct ttagaagaaa aaatccatag agtagagcat
    22981 cgaatctatc cagaagtgat tagtgaaatt attgaaaatg gaggctatct gaaatgacaa
    23041 gagcattgat cagtgtatca gataaaactg ggattgtagc attcgcccaa ggtttgattg
    23101 aacaaggagt agaaatcatt tcaacaggag gaacaaagca tgcgttggat aaagctggga
    23161 tcaaaacgct ttcgatcgaa gaagtgacag ggtttcctga aatgatggat ggccgtgtca
    23221 aaacacttca tcctaaaatc catggtggtc ttttaggaag acgcgatctc cctagccata
    23281 tggaggcaat ggaaacggaa aatatccaac caatcgactt tgtttgtgtc aatttatatc
    23341 ctttcaaaga aacgattatg aaaccagatg tcgaaatagc agaagctatt gaaaacattg
    23401 atatcggtgg tcctagtatg ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg
    23461 ttgttgatcc atctgactat gaagaagtac tttcagaatt acgtcagtat gggcatactt
    23521 cactttcttt cagacaaaaa ctagcggcaa aagtctttcg tcatactgcg gcatatgatg
    23581 cactgatcgc tcaatactta acggattggg tggaagaaaa agaaccggaa aaattgacac
    23641 ttacgtatga acgaaaacaa acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct
    23701 tttatcagtc cgctcttctg gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga
    23761 aagaactttc ttataataat atccgagatg cagatgctgc gctacggatc atgaaggaat
    23821 atgacgagcc aacggtagtc gccttgaaac acatgaatcc ttgtggtatt gggacagcat
    23881 ctacgatttt agaagcgtgg gaagcagcgt atgaagcaga tcctgtttca atctttggtg
    23941 ggatcatcgt tttgaatcga gaagtagatc tgccaacagc agaacagatg cataagctgt
    24001 ttctagaaat catcattgcc ccttcttata cacaagaagc tttggaatta ctgcaaaaaa
    24061 agaaaaacat ccgattactg acagtagact ttgcgcagga agaaaatcaa aatcgagaag
    24121 aaacggtctc agtcttaggt ggacttcttg tacaagatca agatctggca atagaaaatg
    24181 aagaagagtg gaaagtcgtg acaaaacgta agccgactcc agcagaagca aaagcattag
    24241 tgtttgcttg gagagctgtc aaacatgtga aatctaatgc gatcgtactg gcaaacgaac
    24301 gtcagacagt aggaatcgga gccgggcaga tgaatcgagt cggttcagta aaaattgcga
    24361 ttgaacaagc gcaagagaaa atggaaggtg cagttttagc tagcgatgct tattttccaa
    24421 tggatgattc ggttgaatat gcagccaaac atggtataaa agcaatcatt cagccaggag
    24481 gaagcatcaa agaccaagat tctattgata tggcgaataa atacgatcta gcaatggtct
    24541 ttactgatgt tcggcatttt agacattaga gaggagtcgg ataaatgaac attttggtga
    24601 tcggtgcagg tggtcgtgaa catgcaataa gtaaaaaact tttagccagt ccgaaggtgg
    24661 atacggtgta ttgtgcgcca ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg
    24721 ctatcagtga gcgagatcat tcagcgttaa tccattttgt taagaaagag gctattgatt
    24781 ggagtatcgt tggtcctgaa gttcctttgt taaatgggat cgtcgatgat tttatggcgg
    24841 aaggattgaa aatcttcggt ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt
    24901 ttgctaaaca attaatggat acttatcaaa tcccgacagc taaatccaga agttttgaat
    24961 gttacgaaaa agcagcagcg tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg
    25021 acggcttagc agctgggaaa ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg
    25081 cggcagaaca aatgttgaac cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag
    25141 aagagttttt ggcaggagaa gaattctccc ttttggcgtt tgtccgtaat accaaggtgt
    25201 atccgatggt catttcgcaa gatcataagc gggcatttga taacgataaa gggccaaata
    25261 caggcggaat gggtgcttat tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag
    25321 ctgtggaaga agttttaaaa ccagcggctt tgggaatgaa agaactagga cgctctttca
    25381 cgggtgtttt atatgcgggt ttgattgcta cgaaggaagg accaaaagtg atcgaattca
    25441 atgcgcgttt cggtgatcct gaaacgcaag tcgttctttc tcggttgaag agtgactttg
    25501 cgcaagtaat cgatgattta ttagagaatc gtacagtaga gttaaagtgg cagcaagaag
    25561 gatacaatgt aggcgttgtg gttgcagctg ccggttaccc cgaagaatat gagacaggaa
    25621 tggttctacc agattttctg gaagaagagc tttctgttta ctatgctgga gtttcctcaa
    25681 ataacggcaa actagttgga gcaggaggac gtctttattt agtagaagca tacggtgaga
    25741 cgatcaaaga agcacagaaa aaaatttaca ccgcgttaat ggataaaacg acagatggaa
    25801 cattttatcg tacagatatt ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa
    25861 tcagctgctt ccatttataa aaaatacaac aaacataatc ggaagcgccg attttagtga
    25921 agccttttca taaagagtgt ggtacacttt tgttgtgtat ataaataagg aggagttcag
    25981 atgaaaaaaa ttcaattagg tacttctgat cagaaagcag ctgcagtgat cttaggctgt
    26041 atgcgtatca acaatgcgga agatccagta aaagtgattg agacagctta tgaaaacggc
    26101 attgattttt ttgaccatgc agatatttat ggaaacggtg aatgtgaaga aatctttgca
    26161 gatgctctag caaaaacatc aatcaaacga gaagatcttt ttattcaaac aaaatgtgga
    26221 atcgttccag gtgtgatgtt tgatttttct aaaaaacata tcattgaatc tgtagaaaaa
    26281 agtttgaccc gtctgaaaat ggattatgtg gatgctttac ttcttcatcg tccagataca
    26341 ttggtagaac cagaggaagt agctgaagcg tttgatgaat tggaacggtc aggaaaagtt
    26401 cgttattttg gcgtcagcaa tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt
    26461 cgtcaaccat tagtagctaa tcagctgcaa tttggcttga aacatactgg aatgatcgat
    26521 caagggattc atgtcaatat gacagatgaa gcaagtatcg atcatgacgg tagtgtactt
    26581 gattattccc gattgcatga tatgacgatc caagcatggt caccatacca gtatggattt
    26641 tttgaaggtg tatttatcgg gaacgaaaaa ttccctgaat taaaccaaac attagaagaa
    26701 ctagcagcta aatatcaaac gacaccaact ggcttggctt ctgcttggat tttacgccat
    26761 ccagccaata tgcaagtgat cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct
    26821 caagcttcag aaattaatat gtctcgggaa gattggtacg aattgtaccg tgctgctgga
    26881 aacattttac cttaaagatt gtcataagca ggtcaaaagg gctataaatt aaaaagacag
    26941 aataacttga aagctgtttt cagcttcgag atattctgtc tttttttatt gcaaatggaa
    27001 agagtatcat tgtacggatt tcgtttatta gttatttttg tttttaagga aaagctgtgt
    27061 ccgatattct tttggagaaa tcccttgttt tttcttaaat aaataactga aatagttagg
    27121 gtcattataa ccaatttcaa aagcaatatc tgaaatacga tagtctgatt gctgcaataa
    27181 tttttttgct aattcgattc tttgttctgt cagataatcg atgaatgttt ggttaaacga
    27241 ttgagaaaaa atcgtactga aatgagaggg acttaatgca acttgttgag cgacccaagt
    27301 taaggacata tctggatcgg taaaattttc tttgataaaa tccaatgcat ggttcaaaat
    27361 tggctgatat ttgttccttg aaggctgtga gggcagagca gttaagaaag aaatgaacat
    27421 ataaactgtc gaacggtaaa gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga
    27481 tgtgcaatga accatttctt gcgggatttt cttttctttc aggcagacat gctggagtag
    27541 cttagtcaag acaaataaac gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt
    27601 caagtgttcg atgtacttag atacttcgtt tttcgataat gaagacaaat caagtgagag
    27661 gtcaaagtaa aagacagaat caactggaag tttcgcttct tgaatcaaat cctcatagct
    27721 gataatttgt tctttaaggg aactggcggt ttcaagtatc atctgcgtga tctcaaaaga
    27781 tgcaggtaat tcgctgattc tttctgcgac aggaccaatg gcgacagaaa aattattttt
    27841 ttgtcccttt tctaattcat ggaccaacgt ttgggctacc tgataactgg tctcgatcac
    27901 attttcttca agagaatcaa ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa
    27961 gatcacttgt tgattctccc taaataaaaa cttcaaataa tcactgagat gagcgtaatc
    28021 ttcaaagtct gtactgactt ggtttgctgc taataatacg gtcatttttt tccctgctaa
    28081 agagcgattg aaagattcag caagtttcaa tgtttcactg atcgtaagtg agcctttgaa
    28141 caaaccgttc aagaaatgat tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt
    28201 ttctttttgc tgcaaatcta aagcagttac tgctttttct attgcttgtt tcaattcgct
    28261 acttttaatc ggtttaagca gataatcatc tgcctgtacc tggattgctc ctttcatata
    28321 atcgaaatcg tcgaatccac taataaatat gagacgagtc caaggaagta gtttccttgc
    28381 ttcttttgaa aaagtcaacc cgtccatgaa aggcatttgg atatcagtca gaatgatgtc
    28441 gggctgtaag tcgataattg aagacagtgc catctcacca tctccagctt cgccgacata
    28501 agtgattgga taataagaac caaggacagt tatttgttcc cgcaagttat tgcgaatgag
    28561 gtattcgtct tcgatgataa aaactttata catgtttgtt tcctccaatt gttttgggag
    28621 atagagggat catcagtgtg acagttgttc cttctcggta tttgctgtca atcgtgatag
    28681 gtttttgatc atttccgtaa taaagaagga gacgcttgtt tacgttgtaa agtccgtagc
    28741 cgatactgaa atctgaatcg atccctttgg ctaactcttt acgtatttct gccagttttt
    28801 ctgcagggat gccaatcccg ttgtctataa ctttgaattg gatcatctca tcaattttgc
    28861 atgtggtaat acgaataaat cctttgtgac ggacgtactt cgttccatgg tatacagcat
    28921 tttcaatcaa tggctgcaag atcattttta aaatagaaac aggaaaaagt gtttctggta
    28981 tatcgatctt atagtcaagc atatggccgt atcttatttt taagatgact agataatcat
    29041 tgatatggct aatttctttt tcaagaggaa tccagtcttg tcctttgctt aaagacgtac
    29101 gaaaaaagcc ggaaagtgca tgggtcatag tcagagcttc ttcaaagcag tcttgttcga
    29161 tcaaagaaat aatggcatcc aaactgttat aaataaaatg gggagtaatc tgagcttgca
    29221 atacacgaac ttcactttgt gctaaatggt attgtttcaa agcgttctcc cgtaaagact
    29281 ggtttagatc ttccgccatt ttattcatgc tagcggtaag tgtttgtaat tccagtgttt
    29341 ccggcagggt taaacgaaag ccaaggttcc ctgaagaaag ttgttctgac atctcgatca
    29401 gctgattgat cggttcttgg acctttctct ctaaaaagcg acgattcttt ttcgcataga
    29461 aaaaaatggt actaagaact ccaagttcca ctattgttaa tagaatgagc gagcggacaa
    29521 gtgtcgtatt ttttttgctt gctaagtcga tctccacacg aacaaaatct tgtaagatat
    29581 ctgacagcag ttgagtgaca gaatctacct gattgatgat ctcttcattg gtatccataa
    29641 aatcttttgc caagatatta ttgatcagct cgttttgata atcttccagt gtatcgatcg
    29701 tacgtagcga tacatctaac gtactaattt ctttaagtgt atttgtatta tttttgctct
    29761 ctcgaatatc attcctaagt tcttctagga tattttcctc attgaactca tctacggtta
    29821 cttggccaga aatcaagtcc cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa
    29881 ttgcatctgc tttttggata tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat
    29941 ataacagtat gctcagtagc aaagggataa cagcaaatag aagcatggct ttgtttgctt
    30001 gggcaaaaat ctgttttatg cttaaatttt ttgtcatgat acttctccag tcaataattt
    30061 ctggttggga tgttagctat attctgctgg gaaaagacag tctcggaaat aaaaatatct
    30121 ttaggtaatg tttttcctgt ttgaatattt ccaaaataac gagtaacggt attggctaca
    30181 taccagccga catcaggttt acactcgact acacaattga cgattcctga gcgtagcttc
    30241 tcgatcatat cttcttgtcc atcaatagtt acaatgatca aatcttctcc aggtttgatt
    30301 gatgtttctt ttatcgcagc taaagcaccg tgagtcattt catcactatg gctgtataat
    30361 acatcgattt cggataaatc atttgtttct aaatatcgtt tcatctggtc ttttcctttc
    30421 atccgaataa aatcaccgac aattcggtga acaatagtca tcggaggttt atggtcgatg
    30481 gttccatctt tttttagggt tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt
    30541 agcttggtcg gtgtggagtt ctctgaacca gcaagttcta ggattctgat ttcctgtttc
    30601 tttgaatttg tgaagtgatt gctgacaaat aaacccgccc gatttccttc tgctttgaaa
    30661 ctggggccaa tgtgggtgac gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca
    30721 agtatgacag ggatccctgc tcgctttgct tcttttaata cagagtccca cccggattct
    30781 tgcaaaggag taaaaatgat catatccact ttataggcaa tgaacgtccg tatatcttgg
    30841 atttgccgtt cctgattcat gtagccattt cggtagagaa cttggtaatc ttctttttca
    30901 agcgcagtga taattgattc agtatgcttt ttccgccagc tactctccgt tcctgattgc
    30961 gaaaaaccaa cgactagtgg gtcaggtgct tcctttgtat taggtgtaca ccccatcatt
    31021 tggtaagcga ttacaacaag ggctaaaaaa aataaaacac gtttcacttt aaatcactcc
    31081 atctggattt gttagtacta tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc
    31141 aatcgaaaaa aatccaagaa acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa
    31201 tttaaagtaa atcccataaa aaaatcaatg ttaacgcttt ctttattagc tatactcggt
    31261 gtatcaattg aaatggggga taaaaaatga aaaacaaatt tagaaagatt gttgggacta
    31321 tcgctatttt gaccgcaagc attctattaa gtgcgtgtgg caattcagga tctgcagatg
    31381 atagtacggg gtatgtagga atcgctatgc cgaccaaatc agcagaaaga tggatcgctg
    31441 atgggaacaa catggtctcg gaattagaaa aactgggtta taaaacagat cttcaatatg
    31501 gagaagataa agtagaaaat caagtagcac aaatagaaaa tatgattaca aaaggggtag
    31561 atacacttgt gattgcttct atcgatggtt cggctttaac tgatgtgtta gctaaagcaa
    31621 aagaagcaga tatcaaggtg atcgcctatg atcgtttact gatgaattca gaaaatgtcg
    31681 attattacgc gacttttgat aattttggag taggtgtatc gcaagcagct tatattgaag
    31741 agcacttagg attgaaagaa ggaaaaggac cattcacgat tgaattattt ggtggctcgc
    31801 ctgacgacaa caacgcacta atcaattaca acggagtgat gtctgtttta caaccatata
    31861 tggacaacgg tcagttggtc gtgccatctg gacagacgag tttcagtcaa atcgctacgc
    31921 ttcgctggga tggttctacg gcgcaagcaa ggatggataa tctgttaagc gcaaattata
    31981 ctgaccaaac attggatgct gttttgtcgc catatgatcc aataagttta ggaatcattt
    32041 cgtcattaaa aggcgtgggg tatgggagtg aaagtaaacc tttacctgtg attactggac
    32101 aagatgcgac agttgctggc gttaaatcga tcattgccgg ggaacaaaca caaacgatct
    32161 ttaaagacac acgtatctta gcaaaaaata cgattgaaat gatcaaagca atcagtgatg
    32221 gagaagaggt tccggtcaat gatactgaaa cttatgataa tggggtgaaa actgtcccta
    32281 cgtatttagc aaatacagta tctgtcgata aagataatta tcaagcagag ctgatcgaca
    32341 ccgattatta taaagaatcc gatctgaaaa attaagagga accaaacaag gaactaaaga
    32401 gatctaagtc agagagagga taagaattat ggcggattgt attttaaaaa tggcagatat
    32461 caccaaaaaa ttttctggaa tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg
    32521 ggaaatccat gcattgtgcg gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt
    32581 gagcggcttg tatccatatg gctcctatga cggggaaatc ttttatgatg gaaagctgtg
    32641 tcaatttaaa gatttgaaag atagtgaaga aaaaggcatc gtcatcattc atcaagaatt
    32701 agcactaagt ccttatcttt caataaaaga gaatattttt ctcggaaatg aacaagcaga
    32761 aagaggcgtg attgattggg aactgacaga aaagaaaaca tatgaactgc tgaataaagt
    32821 cggtttgtct atcgacccga atcttctagt atcccagatt ggcgtagggc aacagcaact
    32881 agttgaaata gctaaagcat tatcaaaatc cgtccgtctg ttgattttgg atgagcctac
    32941 cgctgcattg aatgaagaag aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga
    33001 gcaaggtgta acctccatca ttatttcaca taagctgaat gaaatcgtgg atatcgcaga
    33061 tcgaatcacc attttacggg atggacagac gattgagaca ctagaaaatg aaatgatcag
    33121 cgaagagcat attattcgcg gaatggtggg cagagattta gttgatcgtt atcctgaaag
    33181 aaaaccggat attggagaag tttattttaa agtagaagac tggacggtct atcaccctct
    33241 tgatcacgag cgaatcataa acgaccacat caacttgcat ttgaaaaaag gagagattgt
    33301 aggaatagct ggactgatgg gagcgggcag aactgaattc gctatgagtc tatttggaca
    33361 ttcttatgga agcaagatat ccggacgtat attaaaggaa ggggcagaga tatcagttaa
    33421 gaccgttcct tcagccattg caaacggact cgcttatgtc tcggaggacc gaaaatcttt
    33481 aggactgaat ctgctgatgg atattcggga aaacgtgacg ctgagcagtt tggataaaat
    33541 aagtcaaaaa ggagtattag ataaagaaaa agaagtaact gaggcagaaa atttccggaa
    33601 aaagatgcgt ataaaaacaa actccatcca tcaaattgtc agtagtctta gtggtgggaa
    33661 tcaacaaaaa gtcgttttat ccaagtggct gatgacacaa cccgatattc tttttttgg
//
LOCUS       DACECR010000022        33711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.022, whole genome shotgun sequence.
ACCESSION   DACECR010000022 DACECR010000000
VERSION     DACECR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.022"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            513..1220
                     /locus_tag="IWO17_000959"
     CDS             513..1220
                     /locus_tag="IWO17_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0289853.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1280..1924)
                     /locus_tag="IWO17_000960"
     CDS             complement(1280..1924)
                     /locus_tag="IWO17_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ0289854.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            2051..2338
                     /locus_tag="IWO17_000961"
     CDS             2051..2338
                     /locus_tag="IWO17_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289855.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2446..2766
                     /locus_tag="IWO17_000962"
     CDS             2446..2766
                     /locus_tag="IWO17_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0289856.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2847..4160
                     /locus_tag="IWO17_000963"
     CDS             2847..4160
                     /locus_tag="IWO17_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0289857.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATIQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4157..4876
                     /locus_tag="IWO17_000964"
     CDS             4157..4876
                     /locus_tag="IWO17_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ0289858.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            5004..5555
                     /gene="raiA"
                     /locus_tag="IWO17_000965"
     CDS             5004..5555
                     /gene="raiA"
                     /locus_tag="IWO17_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ0289859.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5777..8311
                     /gene="secA"
                     /locus_tag="IWO17_000966"
     CDS             5777..8311
                     /gene="secA"
                     /locus_tag="IWO17_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ0289860.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8437..9538
                     /gene="prfB"
                     /locus_tag="IWO17_000967"
     CDS             join(8437..8499,8501..9538)
                     /gene="prfB"
                     /locus_tag="IWO17_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ0289861.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9643..10329
                     /gene="ftsE"
                     /locus_tag="IWO17_000968"
     CDS             9643..10329
                     /gene="ftsE"
                     /locus_tag="IWO17_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ0289862.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGAFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10313..11206
                     /locus_tag="IWO17_000969"
     CDS             10313..11206
                     /locus_tag="IWO17_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0289863.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11358..12806
                     /gene="cls"
                     /locus_tag="IWO17_000970"
     CDS             11358..12806
                     /gene="cls"
                     /locus_tag="IWO17_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0289864.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13143..14000
                     /locus_tag="IWO17_000971"
     CDS             13143..14000
                     /locus_tag="IWO17_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ0289865.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14115..15035
                     /gene="pstC"
                     /locus_tag="IWO17_000972"
     CDS             14115..15035
                     /gene="pstC"
                     /locus_tag="IWO17_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ0289866.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15035..15919
                     /gene="pstA"
                     /locus_tag="IWO17_000973"
     CDS             15035..15919
                     /gene="pstA"
                     /locus_tag="IWO17_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ0289867.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15934..16740
                     /gene="pstB"
                     /locus_tag="IWO17_000974"
     CDS             15934..16740
                     /gene="pstB"
                     /locus_tag="IWO17_000974"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0289868.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16756..17514
                     /locus_tag="IWO17_000975"
     CDS             16756..17514
                     /locus_tag="IWO17_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0289869.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17527..18204
                     /gene="phoU"
                     /locus_tag="IWO17_000976"
     CDS             17527..18204
                     /gene="phoU"
                     /locus_tag="IWO17_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ0289870.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18442..19473
                     /locus_tag="IWO17_000977"
     CDS             18442..19473
                     /locus_tag="IWO17_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0289871.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19470..20237
                     /locus_tag="IWO17_000978"
     CDS             19470..20237
                     /locus_tag="IWO17_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0289872.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            20337..21905
                     /gene="liaX"
                     /locus_tag="IWO17_000979"
     CDS             20337..21905
                     /gene="liaX"
                     /locus_tag="IWO17_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ0289873.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22205..22522
                     /locus_tag="IWO17_000980"
     CDS             22205..22522
                     /locus_tag="IWO17_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ0289874.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            22522..22872
                     /locus_tag="IWO17_000981"
     CDS             22522..22872
                     /locus_tag="IWO17_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0289875.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23039..23977
                     /locus_tag="IWO17_000982"
     CDS             23039..23977
                     /locus_tag="IWO17_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ0289876.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23992..24822
                     /locus_tag="IWO17_000983"
     CDS             23992..24822
                     /locus_tag="IWO17_000983"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ0289877.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24866..25891
                     /locus_tag="IWO17_000984"
     CDS             24866..25891
                     /locus_tag="IWO17_000984"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0289878.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25906..26844
                     /gene="galU"
                     /locus_tag="IWO17_000985"
     CDS             25906..26844
                     /gene="galU"
                     /locus_tag="IWO17_000985"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0289879.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            26969..27409
                     /locus_tag="IWO17_000986"
     CDS             26969..27409
                     /locus_tag="IWO17_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ0289880.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27412..28014
                     /locus_tag="IWO17_000987"
     CDS             27412..28014
                     /locus_tag="IWO17_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0289881.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(28077..29180)
                     /locus_tag="IWO17_000988"
     CDS             complement(28077..29180)
                     /locus_tag="IWO17_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0289882.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            29390..30391
                     /gene="ccpA"
                     /locus_tag="IWO17_000989"
     CDS             29390..30391
                     /gene="ccpA"
                     /locus_tag="IWO17_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ0289883.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            30746..31141
                     /locus_tag="IWO17_000990"
     CDS             30746..31141
                     /locus_tag="IWO17_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289884.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            31141..31392
                     /locus_tag="IWO17_000991"
     CDS             31141..31392
                     /locus_tag="IWO17_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289885.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            31435..31830
                     /locus_tag="IWO17_000992"
     CDS             31435..31830
                     /locus_tag="IWO17_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289886.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            31827..33083
                     /locus_tag="IWO17_000993"
     CDS             31827..33083
                     /locus_tag="IWO17_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ0289887.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            33095..33418
                     /locus_tag="IWO17_000994"
     CDS             33095..33418
                     /locus_tag="IWO17_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289888.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            33411..33677
                     /locus_tag="IWO17_000995"
     CDS             33411..33677
                     /locus_tag="IWO17_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289889.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        11298 a         5796 c         7247 g         9370 t
ORIGIN      
        1 taccacaatc gtaagctctc gttagttcga gagctctttt ttttgctttg ctaccttgtt
       61 ccttgaagat tctgctgatt taagatagag ttaagttaac tacaagaatt ggagggatcc
      121 acatgaaatg gaaagaaaaa gtagacgaag cagctgaaaa gttatatgat ttggtcaaat
      181 cagaaaaata caatattgaa gtgaatattc cgaaaaaagg cggaaaagcg gtccaggtaa
      241 aatcaaaacg cccaacgaat catacaaaaa aatggatggc taaaaaccgc taataataac
      301 aaaagatcca taagtcggca ttttcatcga ttttagggtc ttttgttatt ttctaggttg
      361 aacatatcta aaaaattgtt tctgacaata ggagtaggaa gaacgatgtg tgtctaaatc
      421 ctctgttgat ggatagaaac ctttatcttt ttcagttgtg aatcaggtat ataccagtta
      481 taatagaaga aacgaaagga cgggtgtacg ttatgtcgaa tcaattacct gtatatatac
      541 agattcatga ccaattaaaa aatgaaatcg aacaaggtat atggaaagtc ggagaccgtc
      601 ttccttcaga aagagaatta gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag
      661 caattcaaaa cttggcagat gaaggaatct tagaaagaaa gatcggttcg ggtacatatg
      721 ttgcgcgaga aaaagtacaa gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg
      781 aatcgcaaaa tcgagtaccg tctagtcgga caatctctta tttcttgact tcgccaagct
      841 caagtgaaat ggaaaaacta aacttgagta aagatgacac gatcttgcga atggaacgta
      901 ttcgtttcgc agatgatgtc cctatttgtt ttgaagttgc cagtgtgcca caaaagctga
      961 tcgatggcta cagcaaagca gagattactg aatcgttgta tcgcacgcta gaagaaaaag
     1021 gcgggaaaaa gatcggtcca gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa
     1081 ttgctgacta tttagaggta aaacgaggag atgcagttct tcgtttgcgc cagatttctt
     1141 attttgaaga cggtacgccg tttgagtatg tgcgtacgca atacgtagga aatcgttttg
     1201 agttttattt ggaaaaataa aaaacaggtg atccctgatt ccttacttag aaataaaaga
     1261 gaatcagaat cacctgtttt tatatatgat cagttaccag ttgctcgtga taagaaacag
     1321 gaccctcttc aaattggact tgtccgttca gcagctcaat gatggattct ttaaagtggt
     1381 cagcactttt ttcatctaca gcacagatga cgctaacttg atcagtatat tggatttctt
     1441 ttataaaaaa atgttggagt tcaagataat tctgtaattt tcctagttgc ggatacgtga
     1501 tcatcacaga gatctcctgt tgcaaggtac cttctacgat tccaatgtca gacaatgctt
     1561 gagagacagc atgagtgtat gctcgtatta atccgcctgt gccgagtttc gttcctccaa
     1621 aatatcgagt gactactgca gctacattga tcagttgatt ttttttgagg acttccaaca
     1681 tcggtacgcc agccgttcca cttggttctc catcatcgct gcttcgctgg atttcatttt
     1741 tttctccaat gatgaaggcg ctgcagttat ggttcgcttt ccaatgttct ttttttattt
     1801 cttgaatgaa actttttgct tggtcctctg attctattcg tctaagagaa cagataaagc
     1861 gtgatttttt gatctcaatc tcactttgtc cattttcttt tattgtacga tagcgctcga
     1921 tcatcttgtc acctcttttt ttcattttct ttattcagta taaataaagt tctaggttgt
     1981 ttcaacactg taacggttgc gcaatcgctg tagtatgcga taataaagac aaaggtaggt
     2041 ggatctgggg atgcaggaac gaatgaaaaa gtacgatgca attacgcatt atttgaagaa
     2101 caatggcggc tctcaagtaa cgttgacctt tacccaattt gacgaactgc tgtttcccag
     2161 taacggactt cccaaaacag caagggaaag tacagattgg tgggcaaatg attataaaca
     2221 ccctgaaaaa ggtgcctatg gctggataaa tgcaggctat gaagtagttg tcatcaattt
     2281 ggacaaagaa tatgtcgtgt tcaataaatt agtgaaatct agttggctat ttgattaaat
     2341 aaaccgcgtt tagaaaacgt tgacaaattt ctttcttttt atataataga gtaacagacg
     2401 cgaagaaaac gtttgcgtag tttgttttaa aaaggagaga aaagtatggc taaaaaaaca
     2461 atcatgcttg tatgttcggc tggtatgagc acaagtttac tagtaacaaa aatgcaaaaa
     2521 gctgctgaag caaaaggaat cgactcggat attttcgctg tgtccgcttc tgacgcagat
     2581 aacaatcttg caaataaaga tgtagatgta ctgttattag gacctcaagt tcgctttatg
     2641 aaagctgatt ttgaaaaacg tctggaacca aaaggcattc ctttagacgt catcaatatg
     2701 gctgattatg gtatgatgaa tggcgaaaaa gtgttagatc aagcaatcac gctcataggt
     2761 gaataaatag aacagtataa ctatcgaaca agcgatccaa aaaatagatc gcttgttttt
     2821 tcgtatattt ttttagggcg aataatatga atcatttttg gggtagaagg cttctgacga
     2881 gagatattaa agcaatagac tgtgaggagg taaatttacc aaattatgag aagatcgcag
     2941 cagtggaacg tttgggcaac tggattcttt gtcatcgctg tggaatgagg acgtccgtca
     3001 ttgatggaca gcttgcagat tacggttatt tttgtacaca ttgtctatat ttagggcgat
     3061 gcgacactca acaagattta tatttgtttg atcagcctga agcaaaacct cgagaagtcg
     3121 tctttttttg gacaggacag cttactgaaa aacaaacgga aatcgcaaag agaatacttt
     3181 gccattcaga aaaaagacat catcttattt gggctgtaac aggtgcaggg aaaacagaga
     3241 tgctttatcc tattttagtg gaaactttaa aagctggagg aagagtagcg atttgtacac
     3301 cgcgagtcga tgtttgtaat gaattgtttt tacgctatcg ttatgttttt ccagaagaaa
     3361 aaatcacgtt gctccatggg aatacagcaa cggcttatac ctttacttct tttgttatct
     3421 gtacgattca tcagctatac cgtttttatc ggtcctttga tttaatagtg atcgatgaga
     3481 tcgatgcgtt tccttataac ggggatgcag gattagctca tgctgtgcaa acagcgatta
     3541 aaccagacgg acgattcatc tacttaagtg ccacacctga taaaaagtta ctaaaggaaa
     3601 tcaagcagac gtttgacatc cacaaacttg ctttgcgctt tcatcggcgt ttacttcctg
     3661 aacccgtctt ggttttcatg aataattggc gacaaaaatg caagaacaag aagaagataa
     3721 aatctttggt gcggatcatt catcaattaa ttaaagaaaa ccatatcctt ttattttgtc
     3781 caagtatcac gatgatgcat cgtttgaaag ccacaattca agaggctctt ccgagatatg
     3841 agatcacaga tgtttcttca agagacaatc agcgggcgga aaaagtccag aaaatgcgag
     3901 aagcaaaata tgacatactg ttaaccacga tgatccttga acgtggagtc acatttgaaa
     3961 gaatatccgt tatcgtttta ggagcggatc atccggtttt tactaaatct tctttggtac
     4021 aaatcgcagg aagagccgat cgtaaagggc cttatacgaa cagtcaagta tactttttct
     4081 atgaagaaaa aactgcagct attcgaaaag cttgtcagga aatcaaagaa atgaatgaag
     4141 aagggaagaa gatgctgtga ggtgcagttg ctgcagtaaa gatatcgtcc aaaatttaac
     4201 attcagcgag attattggtg tttcgaaata cagattacct cagacttgtt cagtttgtcg
     4261 cgcacaattt catttattgg aaacaagagg ctgttctggc tgtcaaaaac caatccattt
     4321 caatctagag agtacgaaaa agaaagtaaa gtctattccc gtttactgca gtgactgtca
     4381 agaatggaag aagaaatatc cgacctatga ttttcagcac cgtgcctttt ttgtgtacga
     4441 tgaaaatatg aaggcctggc tcaatcaata caagtttatg ggagatatcc gactagcagg
     4501 cacctttgca tataaatgga cgcagataaa aaaagaatac ccgacataca tctattgtcc
     4561 aataccttta tctgaaaaaa ggcgaacaga aagaggattc aatcaagtaa gcgagatgtt
     4621 gaaagcagca agaattcctt ttcagatgct actcaaaaga gagaagcatc tgctggctca
     4681 agcaaaaaac acgcgaaagg aaagattgga gatgcctcaa ccttttgaac ttgcagtcac
     4741 cttttctaag atacaaaata aaaaaatatt gattattgat gatgtctata caacaggaca
     4801 gactcttttc cacgcagcag attgtttgca ggtcgcacag ccgaaaatca tccgaaccat
     4861 ttctttagca agatgaatta gatgtggaaa tttgatagcg atttaatttg caaataacct
     4921 agagttcgat ataatagaat taagaagagg gaaagagtgg tccttctcct tcttgattgt
     4981 ggtagacgaa aggggtaatt tttatgttta gatataatgt acgcggcgaa aatatcgaag
     5041 taactgaagc gattcgcgac tacgttgaga aaaaagtagg caaactagaa cgctatttca
     5101 gtgacgcacc cgatgcaacg gcgcatgtga acttaaaagt ttatacagaa aaaacagcaa
     5161 aagtggaagt aacgatccca ttaccttatc ttgtattacg tgccgaagaa acttctccag
     5221 atttatacgc aagtatcgat ttggttgttg acaaattgga acgccaaatc cgcaaattca
     5281 aaacaaaaat taatcgtaaa tcccgtgaaa cgggcatgaa tactgcagat gctactgctt
     5341 tattcaatga agaggacgaa aacgaaaatg attctgaatt agatatcgta cgtacgaaac
     5401 gcctttcatt gaaaccaatg gatagtgaag aagcagtctt acaaatgaac atgctgggac
     5461 ataacttctt tatttttgaa gatgcagaaa caaatggtac aagtatcgtt taccgccgta
     5521 aagatggaaa atacggactg attgaaacag actgatcagc cttacttaaa taacaacgaa
     5581 aacttgcagc agctactgac agctgccgca agtttttttg agtttcctcc tcttttagat
     5641 gaaatatgaa taaattatga agttcttact ttaatacgct taagatattt caattatcta
     5701 taactaatga taaaatgaaa agtaaagtct acatagtctg gataatacac agaaggaaaa
     5761 ggagattaca caacgcatgg ccaatttgat tcgttcactt atcgaaaacg ataagaaaga
     5821 actaaaacgt ttagatagca ttgcaaaaaa agttgaagca tacgctgacc aaatggcagc
     5881 attaactgat gaacaattac aggcaaaaac tgctgaattc aagggcagat accaaaaagg
     5941 agaaacatta gatcaattac ttccggaagc ttttgcagtc gtccgagaag cagcaaaacg
     6001 tgtattgggg ttatatccac accacgtaca attgatgggt ggaatcgtgc ttcacgatgg
     6061 aaatatccct gaaatgcgta ctggtgaagg taaaaccttg accgcaacga tgccagtata
     6121 tctaaatgct ctgactggcg aaggggtaca cgtagttacc gtcaatgaat atttagcaac
     6181 ccgtgactcg acggaaatgg gcgaacttta taatttctta ggattaacag tggggttgaa
     6241 tatcaactca aaatcttcgg atgaaaaacg cgaggcttat aattgtgata tcacttatag
     6301 tacgaataat gaattaggat ttgactactt gagagacaat atggtcgttt accgccatca
     6361 aatggtacaa cgtccgctga attatgccat tgtcgatgaa gttgactcaa tcttgattga
     6421 tgaagcgaga acgccgctga tcatttcagg gcaagcagaa aaatccacag ctttatatac
     6481 aagagcagac aacttcgtga aacgtttgaa agaagaagaa gattataaaa tcgatgtaca
     6541 atcaaaaaca atcagtttaa ccgaagcagg gatcgaaaaa gcagaagaaa cattcggttt
     6601 ggataactta tacgacattg aaaatactgc attgacccat cacttggatc aagcattgag
     6661 agccaactat atcatgcttt tagatatcga ttatgttgtc caagacggaa aagtactgat
     6721 tgttgaccag ttcactggtc ggatcatgga tggacgccgt tattcagatg gacttcacca
     6781 agcaatcgaa gctaaagaag gcgtagagat cgaagacgaa acgaaaacca tggcgacgat
     6841 cactttccag aactatttcc gtatgtacaa gaaacttgcg ggaatgactg gtacagctaa
     6901 aacagaagaa gaagaattca gagaaattta taacatccaa gtataccaga ttccgacaaa
     6961 tcgtccggtt atccgtgatg atcgtccaga tttgctttac ccaacattac aaagcaagtt
     7021 ccacgctgtc gtgcaagata tcaaagatcg ctaccgcaaa ggtcaacctg tgttagtcgg
     7081 aactgttgcg gttgaaacat ctgagttatt atcagatatg ctgaatcaag aacgtattcc
     7141 tcatgaagtg ctgaatgcga aaaatcactt taaagaagca gagatcatca tgaatgctgg
     7201 tcaaaaaggt gctgtgacga ttgctaccaa catggctggt cggggaacag atatcaagtt
     7261 aggacttggg gtaagagaac ttggtggtct agcagttatc gggacagaac gacatgaatc
     7321 tcgtcgtatc gataaccagc ttcgaggacg tgcaggacgt caaggagacc ctggtatgtc
     7381 acagttctac ttgtcattgg aagatgattt gatgaaacga ttcggttcag aacgaatcaa
     7441 atctttctta gatcggatga agatcggtga ggaagatgct gttatccaaa gcaagatgct
     7501 gacgaaacaa gtagaatctg cacaaaaacg tgtagaagga aataactatg acacacgtaa
     7561 aaacgtctta caatacgatg atgtgatgcg tgaacaacgt gaagtaatct ataaacagcg
     7621 acaagaagta atcatggaag aaaaaagctt atccaagcca ctaatgaaca tggtcaaacg
     7681 gacaatcagt cgtgtagtag atgcgcatac acaattagag aaagaaaatt ggaatttgga
     7741 aagtatcatc gattttgccg gcagtactct tgtccatgaa gatagtatta ctcttgcaga
     7801 tatcgaagga aaaacagctc aggagatcaa agagtatcta aacgaacgag cacaagaagt
     7861 atttaatacc aaagcggctc aactaaatgg tccagagcaa ctgttggaat ttgaaaaagt
     7921 cgtgatcttg cgtgttgtgg actctaaatg gacagaccat attgatgcaa tggatcaatt
     7981 acgtcagtca atcggcttac gtgcttatgg acagaacaat ccattagtcg aatatcaaac
     8041 agaaggctat aagatgtttg aagatatggt aggcgccatc gagtatgaag tcactcgaat
     8101 ctttatgaaa gctgagatcc gtcaaaacgt acagcgtgaa caggtttctc aaggaacagc
     8161 ttctcactca gaagacggag atgccaaaga aaatagtaat actagcgcga aaaaacagcc
     8221 aatccatgtg aacaaggtcg gaagaaatga tccttgtcct tgtggaagcg ggaaaaaata
     8281 taagaactgt catggaaaaa atgcaaacta atttctgaca gcaggattgg tgagacaaat
     8341 tcttttttta agaattcgtc tcaccttctt tctgttattt gctgattctg ttagactaga
     8401 tataatgaaa aaagtatagt gaaagaagga aaaaacatgg aaatatccga aattcgaaat
     8461 ctcctgacag aaatgcagga aaaggtcaca agcttcagga ggtctctttg acttagacca
     8521 gctggaagaa gacattgcgg aagcagaagc aaaaatggct gaacctggtt tttgggatga
     8581 tagcgaagct gctcaaaaag tgatcagtga aaacaatgca aataaagaaa cttatgatca
     8641 attccattct cttgctgaag aactggaaga aatcgaattg atggtggaga tgttgcagga
     8701 agaacctgat gcagccatgc aagaagaagc agaaacacgt atcaatgcgc tagatgaaaa
     8761 aatgaagaca tatgaattat ctatgttgtt agatggtcct tatgatcgaa acaacgccat
     8821 cgttgaattg catccagggg ctggcggaac ggagtcccaa gattggggca gtatgctttt
     8881 acgtatgtat acacgctggg cagagcagca tgggtatcaa gtagagacac tagattatca
     8941 agcaggtgat gaagcaggaa tcaagagtgt gaccctactg atcaaaggac acaacgcatt
     9001 tggttatctg aaatcagaaa aaggtgttca tcgccttgta cgtatctcgc cttttgactc
     9061 agcgaaacga cgtcatactt ctttttgttc ggtagatgtg atgccagagc ttgatgaaac
     9121 gatcgatata gaaatcaatc cagatgattt gaaaatcgat acgtaccgag caagtggtgc
     9181 aggtggtcag cacatcaata aaacagaatc tgctgtacgg atcactcact tgcctacagg
     9241 aacagtagta gctagtcagg cacaacgttc acaattgaaa aaccgtgagc aagcgatggg
     9301 gatgttaaaa gctaagcttt atcaattaga agtggaaaag aaagaacaag aagcagctgc
     9361 acttcgagga gaacaaaaag aaatcggctg gggttcacag atccgttctt atgttttcca
     9421 tccatattct atggtaaaag accatcggac aaacgtagag acaggaaatg tccaagcagt
     9481 tatggacggt gaccttgatt tatttattga cggctattta aaaatgcatc tacaataaga
     9541 acccattgtt aaattgaaat aaaattgtaa gattttgcaa gcatgttgaa acattgccat
     9601 gctataatag ctaaagactg aaaaagatat ggagagaacg ccatgattga aatgaaggat
     9661 gtaatgaaga agtactcaaa tggtacgacc gctatccgca atctctcaat tgtcatcgaa
     9721 caaggagcgt ttgtctatgt ggttggtccg tctggagccg gtaaatctac attcatcaaa
     9781 ttgatgtatc gagaagaaaa agcttcaaaa ggaactttga acgtcgcagg tcatgatcta
     9841 atagcaatca aaaaccgcga agttccttat ctacgcagag aaatcggcgt ggtgttccaa
     9901 gactataaat tattaccaag aaaaacagta tatgaaaatg ttgcgtacgc tatgcaagtt
     9961 atcggcagaa aacctcggga tatcaaaaaa cgagtaatgg aagtgctgga ccttgttggt
    10021 ttgaaacaca aagttcgagt ctttcctagc gaattatccg gtggggaaca gcaacgtgtc
    10081 tctattgctc gggcaatagt gaatacgcct aaagtactta tcgcggatga accgacagga
    10141 aatctggatc cagaaaactc atgggaaatc atgaaattat tagatcggat caatgcccaa
    10201 ggaactactg tcgtgatggc tacacataac agtacgatcg taaatacgat tcggcaccga
    10261 gtaattgcta tcgaaaacgg ccgcattatt cgagatcaag cggaagggga atatggatac
    10321 gatgattaga acctttttcc gccatttatt agaaagtatc aagagtttga aacgtaacgg
    10381 ctggatgaca gttgcttcag tcagtgcggt aacgatcaca ttgactttag tcggtgtatt
    10441 catggcagtt atcatgaacg caactaaatt agctcaagat atagaaggaa acgttgatgt
    10501 ctctgtattc gtagatatcg gtacaaaaga cgaagagatg aaaaagctag aaaaagaatt
    10561 gaatgactta gatcatgtta aaaaagttga attttcaagc aaagaacagg aattgaaaaa
    10621 aatccaaaac gaaatgggtg attcctggaa attgtttgaa ggtgacaata acccgttata
    10681 tgatgtgtat gtagtaagtg ctactgatcc ttcttataca aaacaagtag cagaagaagc
    10741 agcagatttg aagaatgttt ctcgttctga ttacggtgga gcttcctcag atcggatttt
    10801 ccaaatcgct ggtgcagttc ggacatgggg attagttgca gcggcattgc tgctgttcgt
    10861 agcgatgttt ctcatttcga acacgattcg gatcacgatt ttgtctagac agcgtgaaat
    10921 tcaaatcatg cgtttagtcg gtgcaaaaaa tggatatatt cgctggccgt ttttccttga
    10981 aggtggctgg atcggtctga tcggtgcgat tcttccgatt ttgatcatca catttggtta
    11041 tgcatggttt ttccaattga tcaatccatc attattacgt tcccattatt cattgattca
    11101 tccacaaaat atcgtttgga aaatcaatct tttaatggtc ggcattggtg tgatcattgg
    11161 ttcacttggc tcgatcattt ctatgagacg tttcttgaaa ttctaatacc acaatataaa
    11221 taaaatgaga aagttggaca gtttcgttcc agctttcttt ttttattggc taggagaata
    11281 gaaggaagcg gcttttttcc ctttacttta aaaaacaact tgggtattct taagttggta
    11341 gaagaagagg tgaagccgtg aaaattatcg ttgacaattt ttttacgatc cttttgatta
    11401 tgaatatttt gctttccttt atcatcgttt ttcgagaaag aaaggaaact gctcaaacat
    11461 gggcatgggt gctcgttttg atgttcattc cagttgtagg atttatttta tatatttttt
    11521 taggaagagg gatttccaaa gagaaaatct ttgatttgaa gatccaaggg aaaatcggta
    11581 agaacctgga aattgaagaa gacaaacagg cattgatgcg agggctttat cctcatccgc
    11641 ctactggaca agtagatgtg aaacaattga tctatatgct cacggtattt gaaagtacgc
    11701 tctatacgac taaaaatgaa atcactttat ttacagatgg tcgtgaaaaa ttcgatgcgc
    11761 tgattgaaga tatcaagcag gcagaagacc atatccactt ccagtattat atttatcgca
    11821 gtgatgcatt aggtgaagaa gttcgagatg cattgacaga ggcggcaaga cgaggggtaa
    11881 aagtcagagt ccttcttgat gcatggggtt ccacacaagt ttcttccagc ttttttcaga
    11941 atttgaaaaa agcaggcgga gaagtcgcat ttttctttcc tctatttgtt ccgtatatta
    12001 atcctcggat caattatcgc aatcatcgta aaatcgtagt tattgatggg aaaatcggat
    12061 atacaggtgg gttcaatgtt ggaaatgaat atctaggatt ggtaaagaaa tttggttatt
    12121 ggcgagacaa tcatttgcgt atttacggag aagcggtgta taccttgcaa aaccgttttt
    12181 tgatggactg gaattctcag catacaaaag aagtaggcta ttctcccaaa ttttttccgt
    12241 ccattcactc tactggtgat atagctgttc aaatcgtgac aagcggacca gataccgaac
    12301 atgaacaaat caaaatgact tatttgaaaa tgatttcgtt agccaaacgg gagatattga
    12361 tccaaacgcc ttattacata ccggatggat cgattcatga agcactgaag ttggctcttc
    12421 tttcaggcgt aaaagttcat atccaaatac ctaacaaacc agatcatctt ctcgtttact
    12481 gggcaacgta ttctttcgct gcagagctaa tcgagtatgg agcaaggatc gaaacatatg
    12541 agaatggatt tattcatgca aagacgatga ttatcgatgg tggcattgtt tcagttggct
    12601 cagcaaatat cgatgttcgt tcatttcgct tggactttga ggtcaataca ctggtttatg
    12661 atgaacgaat ggcttcacgt gtaagaaaag ctttttttga agactcgaaa atttctacgc
    12721 atctcacaaa agaaatgtat gaaaaccgag gaataatcat caagatgaaa gaaggattag
    12781 caagattgat ttcgccgctc ctttaaaaca taaaaaggac atgagaacca tcaacagatg
    12841 gagatctcgt gtccttttct aacggggcca tttctgagag tacgtcagaa atggagaaag
    12901 cgtctcgcat gagcgaaatt aactttgggg tatctcttgt tttttgggga aagcaagaaa
    12961 taagctaata tatgctatta aaagagacac atttcaaagc cagtatagca tagaaacttt
    13021 taatgagata gtttttttta caattatgag aatttatttt gttatttaat gattttgatt
    13081 cgttttcatc aagtaatgaa aatgataaaa taaacatact ttaggattaa aggagatttc
    13141 taatgaaaaa acttaaatta ttcttaccaa taataggatt agctattctt ttatcaggct
    13201 gttcaaagtg gattgatcga ggagaatcca ttacggctgt tggctcttct gctttacagc
    13261 ctttagtaga aacagttgct gaaacttacc agacagaaaa tcctggaaaa ttcatcaatg
    13321 tacaaggggg cggaagcgga actggtttgt ctcaagtaca gtcaggagca gtcgacatcg
    13381 gaaactctga tttgtttgcg gaagaaaaat cggggatcga tgcctcagca ttagtcgatc
    13441 atcgagtagc agttgttggg atcactccta ttgtgaacaa ggatattggc gtaaaagaca
    13501 tcagtatgga aaacctgaaa aaactattta caggcgagat caaaaactgg agtgaagtcg
    13561 gtggaaaaga tcagccagta gttatattga accgcgcttc aggaagcgga acccgtagca
    13621 cgtttgaaaa atgggtattg gacggagaaa cagctgttca ggcacaagaa caagattcaa
    13681 gcggaatggt gcgccagatc gttgccgaca cacccggtgc aatcagctat gttgcttttt
    13741 cttatgtttc cgatgatgta gatacgttga atgttaacgg tgtagaaccg acagatgaaa
    13801 atgtgacaac gaacaaatgg acgatttggg catatgagca tatgtacaca agagatcatc
    13861 caaaaaaact gacaaaagaa tttttagact atatcttatc tgatgaagta caagataaca
    13921 tcgttggaga actaggttat atccctgttt ctcatatgaa agtcgaacgt gattggcaag
    13981 gaaatatcgt taaagagtaa tctttacaca aaatttacaa acttagctaa acttttacat
    14041 gttattaaaa ccaaattgat gttcaatttg tacactgagt gagtatgtta aacctgatta
    14101 agggggagtc atctgtggaa gatgtgaaaa aagtattatt aacaaaatca aaacgttcaa
    14161 aaatggaaca gcgtggaaaa ttcatcagtt ttttatgtat cgcgctgatc gtgttggttg
    14221 tattatctat tttttatttt gtagctagta aaggattggc cacctttttt actgataaga
    14281 tcaatgtatt tgattttctg tttggtacag attggaatcc aagccaaatc ggagcaaatg
    14341 ggaaaccaat ggtaggggca ttgcctatga tcacaggttc gtttattgtg acctttttat
    14401 ctgctatcgt tgccacgcca tttgccattg gtgcagcagt attcatgacg gagatctctc
    14461 ctaaaaacgg aacgaagatc ttacagccgg tcatcgagct gcttgtaggt attccttctg
    14521 tcgtttatgg ttttatcggt ttatcagtta tcgtgccttt tgtcagaagt atttttggcg
    14581 ggactgggtt tggtatctta gctggtacat ttgtgttgtt cgttatgatc ttgccgactg
    14641 tcacaacgat gactgttgat gcattaaagg cagtgccacg gcactatcgg gaagcttcat
    14701 tagcgcttgg tgccacaaga tggcaaacaa tctacaaagt cgtgctacga gcagccgtgc
    14761 ccgggatttt aacagcagtc gtttttggta tggctcgtgc gtttggtgaa gccttagcta
    14821 tccaaatggt catcggtaat gcagcattga tgccaacagg tcttactact cctgcttcta
    14881 cattgacgtc catcttaaca atgggaatcg gaaatacaat catgggaaca gttgaaaata
    14941 atgtgttatg gtcattagcg ctgatcttgc tattgatgtc attattcttt aatatcgtga
    15001 tccgtatgat tgggaagaaa ggagagctga aataatgaac gcaaaacgct cagataaaat
    15061 tgctacaggt gttttatata tagtttcggg gatcatcgta ttgattttag cagctttact
    15121 actttatatc ttagttcggg gaataccgca catttcatgg tccttcttga ccgaaccttc
    15181 caaagcctac caagaaggcg gtggaatcgg gatccagcta tttaactcga tttatttgct
    15241 tttgatcacg atgatcatca gttttcctat ttctttaggt gcgggtatct atctttcaga
    15301 atatgctgga aaaaactggc taacagatgt gattcgcacg tcgattgaaa tcttgagctc
    15361 gttgccttct gtcgttgtcg gtttattcgg tttcttggtt ttcgttattc aattccagta
    15421 tggtttctcg attatttctg gtgcattggc tttgactgta ttcaacttgc ctcttttgac
    15481 tagaacagtt gaggagtctt tacaagcggt ccattacaca caacgagaag caggattggc
    15541 cttaggtttg tcacgctggg aaacagtgat aaaagtggtg gttccagaag cgacaccagg
    15601 gattttgact ggtgtgatcc taagttctgg aagaattttt ggtgaagcag ctgcattgat
    15661 ctatacggct ggacaaagtg cacctgcgtt agattttacg aactggaatc cattaagtgt
    15721 ttcaagcccg atcagcattt tccgtcaagc tgaaacatta gctgtccata tttggaagat
    15781 caatacagaa ggcacgatgc ctgatggtgt ttctgtttcg gctggcgctt ctgccgtgtt
    15841 gattattgct gttttactgt tcaacttcgg cgctcgtgcg attggcaaac gattataccg
    15901 aaaaatgact tcagcctaag gagaagaagc aaaatgaaaa aatataactg gaatgaaaca
    15961 aatgtcatca ccatgccaaa agagaaagaa gtagcactat atacggatga tctacatgtc
    16021 tggtatggcg acaatgaagc aatcaaagga gtggatctgg aatttgaaaa aaataagatc
    16081 actgcactga ttggtccttc cggctgcgga aaatctacgt atcttcgttc gttgaatcga
    16141 atgaatgatg ggatcgctaa tacgaaaatc actgggaaaa tcatgtatca aggcgtagat
    16201 attaatgcgc ctcaagtgga tgtttatgag atgcgcaaac ggattggcat ggtttttcaa
    16261 cgtcctaatc cgttcagcaa gtcgatttat gacaatatta catttgcttt gaaacagcac
    16321 ggcgaaaaaa acaaaaagaa attagatgaa atcgttgaaa caagcttgaa acaagctgcc
    16381 ttgtgggacc aagtaaaaga cagcttggac aaaagtgcct tggctttatc tggcgggcaa
    16441 cagcaacgtt tatgtattgc tcgtgcgatc gcgatgaaac ctgatatctt gctgctagac
    16501 gagccggcaa gtgctttgga ccctatttcg acaggaacgg ttgaagaaac acttgtaaac
    16561 ttgaaagaag actatacgat catcatcgtg acgcataaca tgcaacaagc agctcgtatc
    16621 agcgactata ctgccttttt ctatttagga aaagtaatgg aatatgacaa aactaacaag
    16681 atatttacca gaccaaaaat tcaggcaacc gaagattatg tgtccggtca ttttggttaa
    16741 gacggaggcc tcaacatgac aaaagagatt atttcatcta aagatcttca tctgtattat
    16801 ggaaagaaag aagcactaaa agggatcgat ctaagcttca atcaagggga aatcactgcg
    16861 atgatcggtc cttctggttg cggaaaatcc acttatctgc gttctttgaa ccggatgaat
    16921 gacctgattc cgaatgtaac gatcaccgga agtgttttat ataaagacaa agacatctat
    16981 ggtccaaaaa cagatgttgt tgaactaaga aaagaaatcg gcatggtatt ccagcagcca
    17041 aacccatttc ctttttctat ctatgaaaat gtcatctatg gattgaaatt aaaaggggaa
    17101 aaagataaaa aagtccttga tcaagtagtt gaagaaagcc tgaaggcagc gtctgtttgg
    17161 gatgatgtga aagacaaatt gcataaaagt gctttatctt tatccggtgg gcagcagcag
    17221 cgtgtatgta tcgcacgtgt tttggcagtt aatccggaaa tcatcttatt ggatgaacca
    17281 acaagtgcgc ttgatcctgt ttctactggg aaaatcgaaa gtatgctgtt agaattaaga
    17341 gagcggtata cgatgatcat cgtgacccat aatatgtctc aagcttcacg tatttcagat
    17401 aagacggcat tctttttaga tggtcacttg atcgaataca atgatacgaa gaagatattc
    17461 atgaatccag aaaaacaaga gacagaagat tatatttcag gtcgatttgg ataaggagga
    17521 aacggaatgc tacgtacaca atttgaagaa gaattgctga atctccataa tcagttctac
    17581 gaaatgggga tgatggtgag cagcgccgtg cacaagtcag ttcgtgcata catcaaacac
    17641 gataaaatct tagcccaaga agttatcgaa aacgatataa atatcaataa catggaaaca
    17701 cgcttggaga agaaaagttt tgaaatgatc gcattgcaac agccagttac aactgatttg
    17761 cgtatgatca ttacggtcat gaaagctagc tcggatttag aacgtatggc cgatcatgcg
    17821 gtgtctgttg ctaaatccac gattcgcgta aaagggcaga cgagaatacc ggaaatcgaa
    17881 aaagagatct ccgatatgtc cgattatgtg aaaaaaatgg tggacaacgt cctagttgct
    17941 tatgtcaaaa cagacgaaaa agacgcacgt acgatcgcca atatggatca aagagtcaat
    18001 gactatttca atcgaatcta taatgcaaca atcaaaaata tgcaggaaaa tcctgagaca
    18061 gtcatcagtg gaacagacta cttgaatgtt gccagttatc tagaacggat cggagactat
    18121 gtgacaaaca tctgtgaatg gatcgtctac ttggctacag ggaaaatcac tgagttaaat
    18181 acgaaccaca atgagatttt ctaaactgaa gctgtgatta tgccgtttgt ttaggaaaag
    18241 aggttgtgaa atcagcggtt gtgaaaaagc tgttctgcat caagtaataa gaacagtcaa
    18301 acaaaaaata gctcctcata tttttcgttt ggctggactt attaccgcag atgcaagctt
    18361 ttgaacaccg tttattcggg aaagggtttg tgacaaaaca tgtcacaaac ccttttcatc
    18421 atttcataga aaggataaag aatgcaaaaa aaaattagca tcatcactat attttttatt
    18481 tgtgtgctta cttcatgggt tattcagcat caagccgctt tatatgaagt acctgtcatc
    18541 gagatagaaa aagtacgaat aaaagaggat catcaattga ttacaggaaa actgatgaac
    18601 acggatcaaa aaggagaatc gatttattta tcggttcctt atcaaaaaaa tcaaatagaa
    18661 acagcaaata taaaagcgag acaaatgtat ctagtccaaa tgcatggaga tgaatggcag
    18721 ctagtagcaa aaaaaaatga tggctggctt ttcttcttca caagtatctt cattggcgcc
    18781 atgttgctta ttggtggaaa acagggtcta tttgctttag gaagtctcat catcaatatc
    18841 gttttacttt tacttttgtt gctcatcttt actaaaacag aagggatttc tctattaaat
    18901 atatctatcc tgttcgtcgt tatagggatg attattagca tattggcttt agatggatgg
    18961 aatagaagca gtataatcaa aatttcagct gcagttacga gtgttttttt agcatttttt
    19021 atttgtcttt tgacaatgaa tcattggaaa gaccaaggac tgcgctatga ggaactgaat
    19081 tatctgacac gaccgtatcg ttctgttttt ctttctagcg tactgattgg aacagctgga
    19141 ggaacactcg ataccgttat cacagtcatt gcgacattag aagaattgac taagcgacaa
    19201 ccagaaataa cagcgaaaca gttatggctt tcaggaaggc aagtgggtcg agatgtgatt
    19261 ggctcgatga ccaatatcct tttgttcgtc tatatcagtg ggtcagttcc atcgcttctt
    19321 ttatatttag gaaatcaatg gtcgttcaaa gaaacgatag aacagcattt atcattggaa
    19381 tttttacgcg tcattgctgg cagcttagga atagtattat cgatccctat ctcggtcctc
    19441 ttttttctga ctgtaaggag gctgaaacga tgaatgtact tattatactt gttgggattt
    19501 tagcaatcag cgtgctactt attggcggta cgcaaggtat gtatattctt ttaggcttat
    19561 tcatcaattt agggatcttt ttcctgttgt tattcggtta tcatcagaag tggccgatac
    19621 tggcattgtc ggtcatagga tttttattga ttgctgtggt tatcttattt tttatcaacg
    19681 gttataatct aaaaatgcgt gcagcttttg tgagtatact gatcttttta ttttgtttcc
    19741 tactattgct tcctatcact gattttttag caatccaagg ttttacatcg gtagaactag
    19801 aagaattatc tggcttagat aagactttag cgattgattt tagattactt acgagatcgt
    19861 tgcttctgat tagtcttagt ggtgctgttt tagatgcgag tgtagcaatt tctagtggga
    19921 cattcgaagt atatcaggca aatccgcaat tatcttttta ccaattgcgc cattctagtt
    19981 tcgctattgc taaaaaaata ttggcgtcaa cagtaatgac acttcttttt gcattcatgg
    20041 gaagttcttt agcgttgatc ctctggttta ttgatttaga cattcctttt cagcaagtga
    20101 tcaatgaaaa atcatttgtt ctagaatata cgatggctat tcttaccaca ttgtccgcac
    20161 tacttgttct tcctttaacc tgcgtgacta cagcatatct ctttacaaaa aagaaagaac
    20221 aactcaagat ggagtaaaag ttacaactta agtcctatga catttttact gaatcagtgc
    20281 ataatatgga tgtaaggaaa agcttgcaaa caattttgct taaggaggca atttccatga
    20341 aagaaagaga acgtatttta gatttagtga aaaaaggaat cctttcgact gaagaaggct
    20401 tagatttatt agaaagcatg gcaactgaaa aagacgaaaa gcaaatcaaa aaagaagcag
    20461 acaaggtaac tgctagtcat aaagaaaaag atcaggcaag tcaattgatc gataaacttg
    20521 aaaacggcga agaagaaatt tctgagccag ttgatccaaa ggaaagagaa agacaagatc
    20581 aagaaaactt agaaaagatc ttagatgaac tagcaactga agcgaataaa acttcggcgc
    20641 gtctagatga agtaaatgct caattcgctg acaataaggc agcacgcaat gagaaacagg
    20701 aagcattgat gcagctgaat acaaaagaag aattaggcga gttgacagaa gaagaattag
    20761 cacagcgtca gacgttagaa gcagaaatca aagaattaga agctagtggc gatacactgt
    20821 tagaagaaca attgaaatta gaagcagaat taaaagatat ccgtaagaat caatggtctg
    20881 agaaaaaaga aacgtttact gataagtttg aattgccaga tgattggaaa gatcaagcaa
    20941 cagatacact gaatcaagta ggcgaaaaaa tgtcagaagc tggatcacag ttaggcaaat
    21001 tcttgaagaa aacattccaa actgtttcag aaacagtgaa tgacaacatg gaatggaaag
    21061 atgtcagctt acgtgtccct gggattgcga caacgaaatt tgagcatgaa ttttactatg
    21121 aagctcctgc agcaagtatc cttgatatca aggcagcaaa cggaaacgtg acgttaaaaa
    21181 cgtgggacag caacgatgtg aaagttgaag ctaagatcaa actttacgga aaaatgggtg
    21241 ctgaaccatt tgaagcattc tctgaacgga gccagattga ggttaatgaa gatcatatct
    21301 ccttccaaat tccaaataaa cgtgtgcgtg cagaccttgt tttctactta cctaaacgag
    21361 tatacgacca cgcagctatt aaacttctaa atggcaacat catgattgaa acattggaag
    21421 ctaaagatat ttatacaaaa tctacgaatg gtaacatcat cgtggaccaa ttaactgcaa
    21481 cgatgctgga agtagaaggt gtaaacggca atatcgatat tcgaaacggg aacatcttag
    21541 attctattat cgaaacagta aatggtacag tgacatttgg tgcgacagca gaaaaccttt
    21601 ctgtgtcttt ggtaaatggt gatgtacgtc ttacgattaa agaagataat ttgaaaaaag
    21661 tagaagcaag ttcagtgaac ggaaatgtca aagtcgcttt accagatggc atcggtttgg
    21721 aaggacatgc taaaacaagt cttggaagta tcaacagccg tctgtccaat tacgaagtag
    21781 ttcgtgagaa aaaagaacgg acaaaccaaa tgttgcaatt ccgtcgtgtt tcagatggtg
    21841 aaatcgcgca agtgcaactt tcaacgacaa caggaagtat ctatttaaaa gatacagata
    21901 aataaagagg ttgtgaaaga gatgttcagc cttgagcaat aagaagcgat tttgtaaaat
    21961 agcttcccct attttacaaa attgtgcctt attgccgata gtctatctct tgaacaccgt
    22021 ttaccaagag attatgaacc tgccaattat cgccgagtca caaggaataa tttcagaaaa
    22081 agctcgtcac gttttatgaa tatggtaatt tgtgaccgag aattttgcag aaagacaccg
    22141 attaagaaga aggttgtcac aacgattttc ttcatttaaa atagtagaac agaggtgaga
    22201 catgatgaaa aaaaagttga caaaatctaa taaaaatgtt gttttgacag ggactttagc
    22261 aggaatagga gaatacatcg ggattgatcc gactgttatc cgtgtgcttt atgtattcct
    22321 gagcttagtt gcttttggaa gtccaattat tttatacatt ttattagctt tgattatccc
    22381 atctgcgcgt tctgcacgaa caaactatgg acataacaat gcatattatg aaaaaaacaa
    22441 ttatcgtgaa caaactcgtg aacaaacaaa aaaacgcaaa gaagcggaaa aaatcgatga
    22501 tgacgaatgg agtgactttt aatgtcgtac tttcaacgta ttttagttaa tacgttaaca
    22561 ttcatatcat tggctgttct ttttcctgcg atggtccatg tgagcagtat ttggacggcc
    22621 gttcttgcca gcttcgtatt gtctgtgttg aatatgtttg tcaaaccgat tttgacgata
    22681 ctgtccctgc cctttacatt agtcacattg ggattgttta cgtttgttat caatgcagcg
    22741 attcttaaaa tgacctcctt ttttgtggga gcagaacatt ttagtttttc ttcattcggt
    22801 gcagcgttgt tagtagctat cttgatgtcg cttgtcaatg taattgtcag cgaacataac
    22861 agtagaaaat aaaagaaagc tgtgccgaac tatcggcgca gtttttttat ttagaatcag
    22921 gaatgcgttt tctattacat tggtaattaa ataaaaacgt tttttttaat tggtagtact
    22981 tttggctaga caatgataaa atgatagaga acactctaat agaaatgagg tagcatgaat
    23041 gtcagaagtt gtaaaagtta gccaattagt gaaaaaatta tctttggaaa tcgtgacagg
    23101 cgacgagaaa agcttagacc gtgtcatcgt aactggggac atctcacgcc caggactaga
    23161 gttgactggt tattttaatt attattccca taatcgttta cagctatttg gcagtaaaga
    23221 aatcagtttt gcggaacgga tgatcccaga agagcgtttg atcatcatga gaagattatg
    23281 tagtgaagat atgcccgcat ttattatttc aagaggatta ccagctccag aagaaatgat
    23341 ccaggcagca aatgaaaatg ggatagctgt actgcgttct cccatctcaa cttctcgttt
    23401 attaggagaa ttatccagtt atttagatgg tcgccttgcc cctcgaacaa gtgtccacgg
    23461 tgttttagtg gatgtttacg gattaggtgt actgattcaa ggtgacagtg ggataggaaa
    23521 aagtgaaaca gcgttggagt tgatcaaacg cggccatcga ttgatcgcag acgatcgagt
    23581 agatgtttat cagcaagatg aattaacggt gatcggagaa ccgcctaaaa tcctggaaca
    23641 tctgattgaa atccgaggaa tcgggatcat tgatgtaatg aatctttttg gtgcgagtgc
    23701 ggtacgtggc tctatgcaag tccagttggc tgtttatctg gaagcatggg caaaagataa
    23761 gaaatacgac cgtctgggct ctgaagacac gaccgttgaa atcgcggaag tgggcattcc
    23821 acaaataaaa atccctgtca aaacaggacg aaacgttgca atcatcattg aagtagccgc
    23881 gatgaatttc cgtgcacgta caatgggctt tgacgcaacg aaaacatttg aagaaagatt
    23941 aagtcgcttg attgaagaaa actcaggcaa cgactagcaa ggagtgacaa tatgttagca
    24001 caaatcaatc gagttgcttt tgagcttgta gggattccta tctactggta tgcattgatc
    24061 atcgtatcag gtatcattat cgctatgtgg cttagcagca gagaggctgt tcgggtagga
    24121 ttaaaagcag atgatgtgac agattttatg ttgtggggat tgcctatctc cattattggc
    24181 gctcgcttat attatgtttt atttgatctt ccccagtata tagctgatcc aatccaaata
    24241 ttcaatatcc gttcaggagg cttagctatt tatggcggac tgattgcagg cgcgatcacg
    24301 ctgtattttt ttactaaata tcattttatt tctttgtgga cttttttaga tatcgctgca
    24361 ccaagtgtat tattagcaca ggcaatcggt cgttggggaa atttcatgaa ccacgaagca
    24421 tatggaccag acacgacgcg aagcttctta gaaagtctgc atttaccgca attcatcatt
    24481 gataatatgt atatcgaagg gatctatcgt caaccaacat ttctttatga atcactttgg
    24541 agtttgattg gatttatttt gcttttgttt ttacgaaaga aaccacacct tttgaaaaaa
    24601 ggcgaagtcc ttttatgtta tctaatgtgg tatagtttcg ggcgtttctt tattgaagga
    24661 ttacgtacag acagtcttta ctttttaggt gtgatccgta tttctcaatt attatctgct
    24721 gtgttatttg tcggtacatt gattctattt atttggcgca gaaaaaaagg aaatttaccg
    24781 gattatgacc gctcattagg aaaaaatcaa caaattatct gatttttaga gcgttaaaaa
    24841 tgaaaaaaat ttcaggagtg gtaaaatgaa ttatagagta gcggttttag gaccaggctc
    24901 ttggggtact gctttggcac aagtcctcgc cgaaaacgga catgacgttc ggatttgggg
    24961 acatcatgct tcacaagtag aagaaatcaa cacccaacac acgaatcaac gttatttacc
    25021 agatcattat ctacccgatt ccatccaagc attcaccaat atggaagatg cagtaaaaga
    25081 cgcagatgcg gtgctattcg tcattccaac gaaagcaata cgttctgttg caaaagaact
    25141 agtgcctaaa ttgaatacaa agccagtgat catccatgca agtaaaggat tagaacaggg
    25201 aacacataaa aggatctctg acatcttgtt agaggaaatc ccagcagaaa aaagaaaagc
    25261 tgttgttgtt ctttctggac caagccatgc agaagaagta gcaaaacatg acatcactac
    25321 gatcacagct gcaagtacag atgcaaaagc agcaaaatat gtacaagagc tgttcatgaa
    25381 taattatttt cggatctata ccaatcctga tgtgatcggt gtagagatgg gggcagcttt
    25441 gaaaaatatt attgctattg gtgctggcgc tattcacggc ttagggtttg gcgataatgc
    25501 aaaggcagca atcatgacgc gcggattagc tgagatcagc cgtttaggtg tagcaatggg
    25561 tgccaacccg ctgaccttta tcggattaag cggaataggc gatctagtcg ttacttgcac
    25621 aagtgttcat tctaggaact ggcgcgcagg caacttgtta ggacaagggc agcccctaga
    25681 agaggtgcta gccaatatgg gaatggtcgt tgaaggtgtg aatactaccc aagcagcgat
    25741 ggaactagcc caaatgatgg aaatcgaaat gcccatcact caaacaatct atgaagtgct
    25801 gtatgaacaa aaagaaatca aaacagccgc aaaagaaatc atgttacgtg atggaaaaaa
    25861 agaaaatgaa ttctcattaa caattttttg aaggagcgaa tgtatatgaa agttaaaaaa
    25921 gcagttattc cagcagcagg attaggaaca agatttttac cagcaacaaa agctatggca
    25981 aaggaaatgt tgccaattgt tgataaacca acaatccagt tcatcgttga agaagcgttg
    26041 aaatcaggta tcgaagacat cttgatcgtt acaggaaaag ctaaacgacc aatcgaagat
    26101 cactttgatt caaatattga attagaaaca aacctaagtg aaaaaggaaa gaccgaatta
    26161 ttgaaattag tagaagaaac gacagatgtc aacttgcatt tcatccgtca atctcatcca
    26221 aagggtttag gacatgccgt tcttcaagca agagccttta ttggaaatga accatttgtc
    26281 gttatgctag gcgatgatat aatggaagac gaagtaccat tgacacagca attgatcgat
    26341 gactatgagc gtacacacgc ttctacgatt gccgttatgc aagttcctca cgatgaaact
    26401 tcaaaatacg gaatcatcaa cccaggggaa gtactggaag atgggttata caatgtcaaa
    26461 aactttgttg aaaaaccaga tccaagcaaa gcaccaagtg atttagctat tattgggcgt
    26521 tacttattga ctccagaaat ctttgatgta ttatcagaac aacaaccagg tgctggaaat
    26581 gagatccaac taactgacgc aatcgatacg ttgaataaaa cacaacgcgt atttgcccgt
    26641 aaattcacag gtaaacgtta tgacgttggt gacaaatttg gttttatgaa aacaagtatt
    26701 gaatatggtc tgacacatcc agaagtaggg gcacctttgc gtgaatacat catgtcttta
    26761 ggtaaaacat tagaagcaga aggaagcaag atcaatcata atccaaaagg aataacagaa
    26821 atcaaagaag aaaagaaaaa ataatctttt tttctaacga gcttcagctg ctaattgaat
    26881 aaaaagtagt tgaagctcgt ttttatttgt ttaaaaaaca cgaaaatgct atccttatag
    26941 aaaagagaga caagaaggga gtttgatcat gtcaggagga gaaattgcag gattgatcgc
    27001 agcaatcgcc tttgcgatac ttgtcgtttt catcgttcgc gtattgttgc aagtttccaa
    27061 aactttagaa aaagtagacc agactgtcgc agaagcaaat acgacaattg agatcgtcac
    27121 acaagacgta gatcttttat ctagacaagt agaaggcttg ctagtcaaaa gtaatgagct
    27181 gctcttggat atcaatggaa aagtcgcaac gatcgatcca ttgttcacag cagttgctga
    27241 tttaagtgaa agtgtatcgg aacttaatca ttctagtaaa aacatcgtga cgaaagtcgg
    27301 cggaatcgga aaaacgacag ctaaagcgac tgttgccgga aaagtcggtg gaacagcgat
    27361 gaaatttttt aaaaacaaaa aacatactga aacgacagga ggaaaataag aatggctaaa
    27421 aaaggtggat ttttattagg tgcagtagtt ggaggtacag ccgcagcaat cgccgcatta
    27481 ttgttagcac ccaaatctgg taaagaactt cgggatgaat tggctgctca agcagatgat
    27541 ttgatggaca tggcttcaga ttacacagat atggcaaaag aaaaatcatc tgagttaaca
    27601 ggtattgcaa aagaaaaagc gcaaggtctt tctcaacaag caggggatct agcaggcaat
    27661 gttaagcaga aggtaagtga atcagcagag gaagcaggaa gtgaatcaga tgatatcttg
    27721 ttaaacctca aagaacaatc agctgattta tctaatcgtt tcaaaaaaac agcagaagat
    27781 ctgaaaaagc cagcagacga attaggtagt attgctaaag atacttcaga agatatcatg
    27841 atcgatgtaa aagatgctgc agataaagca ggagagacgg tcaatgaagg aatgtcagaa
    27901 gcaaaaccag ttgcagatga cacaaaagaa atgttgaaaa tggcaaaaga agatgcaaaa
    27961 gaagctaaaa aagaaatgaa agatacaatg acagattcga ctaccaatca ctaagattac
    28021 tcatttctag aataaaaaag gtgagagcgg accagctctc acctttttta ttttcattac
    28081 tctaaaacaa gtaattcttt tggtgtagtc gtgaatggca cacaaccatc acttgtcaca
    28141 tacacacaat cttcgatacg tacccctact ttttcaggaa tatagattcc tggttcaaga
    28201 gagaagcaca tcccttcttc aatcactaag tcgtttcctt ctacaagaga agggaattcg
    28261 tggacggttg ttccgattcc gtgacctaaa cggtgattga aatattcgcc gtagccatag
    28321 ctgttgatca cgtttcgtgc gatttgatca agttcacctg ctgtcacacc tgggcgaact
    28381 gcctcttgtg cggcaagctg tgcttcaaga gtgatgttat agattttttc ttggaactca
    28441 gttggttttt gataagcaac tgtacgagta gcatcactgc aatagccatt ccaaataacg
    28501 ccaagatcga ataacactag ttcatttggt gaaaccttcg tgcttcctgg tgtaccatga
    28561 gggcttgcag catttgctcc tgcaaggact aatgtgtcga aagacatttg tgaaacacct
    28621 tgttttttta attggtattc gatttctgcg ataattgctt gttcagttac gccttctttt
    28681 actgttttga agccaatttc aaaggcaaca tcggcccatt taccagcttc gatcagtttg
    28741 tctttttctt ctaccgtttt gatcagttgc agacgctgga taatagaagt tagatcggtg
    28801 taagaatctg tacctgtcaa gatatcagag atagcatcga agcgatcgac agtcagagca
    28861 ttcttttcaa tacctacatt accaggttgc ccatagcgtg cgcgaagctc ttttccgatg
    28921 atctcccaag gattttcgct gtctaagtag ccacgtacat cgtacgccca gctgctgttt
    28981 tgagcatctt caacttccag agcaggagtg aaaaggaaag gatcttgttc cagagggata
    29041 aaaagagcta gtacacgctc gtgaggatca ctttcatatc cggaaaagta agcgatgtgg
    29101 ccagggtcgc tgatataagc caattctact tggtgttgtg ccatccattg acgtagttca
    29161 ttgattttta cgttattcat ttggcaacct actttctaaa ttaattttgt attcaataat
    29221 tgtaccactg attgattaaa agaccaacgt tcttaaaact gaaaaaagta aaaattttca
    29281 tgaaaacaga gataaaacgg ttgcaattgt tgagattatt tgatatccta ttttagaaat
    29341 gtaagacgta ttttcagaaa acatataaaa tatgaatagg agaacacaaa tggaaaagca
    29401 aacaatcact atctatgatg tagcgagaga ggcaaatgtt tcgatggcta ctgtttcacg
    29461 tgtcgtaaat ggcaatccaa acgtcaaacc agcaacaaga aaaaaagtct tggaagttat
    29521 cgatcgcttg gattatcgtc caaatgcagt cgcacgtggc ttggcaagta agaaaacaac
    29581 aacagttggg gtaatcattc ctgatgtcag caatatgttc tttgcctctt tagcacgtgg
    29641 tatcgacgat gtggcaacaa tgtacaaata caatatcatc ttagcaaact cagatggcaa
    29701 cgatcaaaaa gaagtcaatg tattgaataa tttattggct aagcaagtgg acggcgtgat
    29761 ctttatgggt caccatatca cagacgaaat ccgtggagaa ttttcacgtt caaaaacgcc
    29821 tgttgtacta gctggttcca ttgatccaga tgaacaagta ggtagcgtaa acattgatta
    29881 tgtgtcagca acaaaagatg cagtaaataa attagcaaaa agcggcaata aaaaaatcgc
    29941 ttttgtcagc ggtgcgctga ttgatcctat caatggtcaa aaccgtctaa aaggctataa
    30001 agaagcatta agcgaaaaca atctaccata tagcgaagga ttgatttttg aggcacaata
    30061 caactttaaa gatggtctgt ctctagttga ccggatccat aatagcggtg caacagctgt
    30121 ctatgtttca gacgatgaat tagccattgg tatcttggat ggtttattag accgcggcgt
    30181 aaaagtacca gaagaattcg aaatcatcac aagcaacaat tctttactga cagaagtagc
    30241 tcgtcctcgt ttgacaagta tcacacaacc attatacgat ttaggagcgg tatccatgcg
    30301 cctattgacg aaaatgatga ataaagaaga agtcgaagaa aaaacgatta tcttgcctta
    30361 tggaatcgaa gaaaaaggat caactaaata ataaaagatc aatcatttaa aggttgttcc
    30421 aaaagtggtc aacttctgga acaacgttta tacgagaaag ggggttatga caaaattttg
    30481 tcataacccc tttttttttg tgactttaaa aaaagagaaa atggcggaaa caaaccttac
    30541 gtccatcgta tacgacacaa tctgtgcgta gatgctacat tttgttcact actatcaaaa
    30601 ttactttaaa atgaaaacgt gataaaaaag aaagtagaaa agtagataag taactctaaa
    30661 aaatccaaaa aaatccaggc tcgattgcgt acttatctta aaggagaaac aatccatcat
    30721 atataacccg aaaggagact tctatttgcc taagaaaaga cgaaagacca tagtcatcat
    30781 tttgaccatt cttactatag gaatcggagg gaaattttac atggacaaaa gagaaatgca
    30841 aagacagaaa gatttactcg cagtagaaaa acagtccgtc aaagttctga aaaatacctt
    30901 tgcggatatc aaggaagtga agattgaaaa atctgctcga aatgaaatga cgggttcata
    30961 ccggatagtt attctaatga ctaataaaca agatcaatcg atttattttt cttatagttt
    31021 ttggaaagaa agaaatgaga taggaagtta cggaatagtt gatgaaaaaa agcaaaaaga
    31081 aggaaatacg ctcaataaag ttaaagtaac ttactctaat ggaaatgagg aaagtattta
    31141 atgattactg acaaacaata tagtgaatta agtgatgctg tttactggct agatcctaaa
    31201 catagagact atgtacctga aatgcaagaa aatttaagtt ttaaaattaa cggcactatg
    31261 tataaaatcc taaaaatcaa aaactccttc gacggcatgc aggcgatggc tgtagcgcca
    31321 atcgtccata gcaaattgga gaaaaatttc aaaaataaaa aaattcctgc aaactttcgc
    31381 gtacttaaat aatgaaggtg tcaatactta cataaaaaga aaggaggcac gttcttgtca
    31441 aggaaaataa gaaaactcag cttagccatt ttattaatcg tcatattagg gatcggaggg
    31501 aaagtctata tggacaaaag agaagcacaa aggcagaaag atttactcgc agtagaaaaa
    31561 cagtcagtca aagttctgaa aaataccttt gcggatatca gggaagtgaa ggtagaagaa
    31621 tttaaaaaaa accctgttac cggaagttat ggtgcacttg tgacaatgac aaataatgaa
    31681 gggaaatctg tttattttga ttatagtttt tggaaggaga gaaatgaact tggtggtttt
    31741 ggcattgaga acagagcggt acaaaagaca ggagtcacta taaaaagagt aaaagtaatt
    31801 ttttctaacg gacaggagga gttagtatga caataaacga taagcattac aatgatatta
    31861 gtgagaaggt ttattggcta gatcccaaat atccccgcta caatgaggga tacaaaaaga
    31921 actctgttaa agaatttgct ggtatggaat ttcaaatcct acaaatcaaa gactcattgg
    31981 acggcatgca ggcgatgtct gtagcgccaa tcaagaacgg taaagtggat ctttctcaag
    32041 tagtgattgc ttttgcagga acgaacttaa gtgattggaa agatttggaa acggatgcaa
    32101 gaagtatcat gaatctctac gggaatagac caggagcaag tttttctgaa aaagcaacaa
    32161 caaacaagat tttatccgtt agtggattga aaacagcggg gcaaatcaat tcagcgattg
    32221 catttgctaa tgaagtaaaa aaggaatatg gagaagctgc tatttctact accggccatt
    32281 cattaggtgg attcttagca ttatatgtag cagcagagaa acaatggaaa aatgtcggct
    32341 tcaacgggcc ggacccgtat gatctcttat caccagaagc aaaaaaatgg gtggaagaaa
    32401 atcccggtat gctcaccaat taccgaaatc acgccgattt tatcggtaat tttggtggta
    32461 atggcacagg tgctgaagtt cggatcagta tggaaacagc attcttgaat ctgcttgctt
    32521 cccacagttt aacctcttgg aagtttgatg aaaatggaaa gctgattatt cctgaaaatg
    32581 aaaacaatgt taaagctcga aaacaacaga aagaaaacca agcgacccag ttgttttcct
    32641 atcagttgca agaactgaac aacatacgta aaaaactacg attaagtggc ggccagctga
    32701 cagttaatga acaaatttac ttagatgaca cacaagctag gttagtagtg gaaaagttat
    32761 cggcaaaaat gaaaagcgct atggaagaag ttatccgcat caatcaaaga ggaatttttg
    32821 agctagagaa aaaatggcaa gaaggcttgc ggcaagcacg tgttgtagcc ccttcgttga
    32881 ctgaacagga aatcatagaa gcattagcta gtgtgggcgc cacaaaagaa aacttagtcg
    32941 attctgcgaa agaagtctat ctgctacgaa tcaataaggc aagacgtatg ggggaggcct
    33001 ttgatacatt ggcgaaggag atccaagaca aaatcaatga aatcgtggca cgagatcgag
    33061 aattagcaca gcaattcaat taaaggagaa gtagatgaaa ctagaagaat tggaaagaga
    33121 ttatcgaagt gaacgaagac aattagaagc tcaagaagat gaattgatcc actttaagaa
    33181 tcaaggatta cgattgatgg agcaagagga gcagcgactg catcatgtat taaagaactt
    33241 agctttagat aatgagcctc tgtttcaagc aagacgaagt tattcgaatt atcaggagca
    33301 ttatcaacaa gccctacaag tagaacgacg aaaactctat cagcagcaag atgaactaga
    33361 acgttcctat cgtcaaaaaa tcagacaaat aactatggag aaatcatctt atgaatgaat
    33421 ttttaatgca attactaaaa gaattagaga aaaatctaca tcgatcaaat gtacgttcag
    33481 ttggtatggg gatatctcag caaatttggg cagaaaaacg attgacagat caaaactacc
    33541 aaatgactgc agaagaagct gctcaagaat tttctaaccg acatgtacac ttgaaaggat
    33601 cagtaaaagg gcaatttgca gaaaaactaa ctgaattatt ccaaagacaa gaaaagaaat
    33661 taaaacagtt tcgttagtaa agttatatag aaaaattttg tgaaaatggt t
//
LOCUS       DACECR010000023        31485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.023, whole genome shotgun sequence.
ACCESSION   DACECR010000023 DACECR010000000
VERSION     DACECR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.023"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(38..505)
                     /locus_tag="IWO17_000996"
     CDS             complement(38..505)
                     /locus_tag="IWO17_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ0289890.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(625..2163)
                     /locus_tag="IWO17_000997"
     CDS             complement(625..2163)
                     /locus_tag="IWO17_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ0289891.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2243..2929
                     /locus_tag="IWO17_000998"
     CDS             2243..2929
                     /locus_tag="IWO17_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ0289892.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3130..3657)
                     /gene="msrA"
                     /locus_tag="IWO17_000999"
     CDS             complement(3130..3657)
                     /gene="msrA"
                     /locus_tag="IWO17_000999"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0289893.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3741..4418)
                     /gene="pgmB"
                     /locus_tag="IWO17_001000"
     CDS             complement(3741..4418)
                     /gene="pgmB"
                     /locus_tag="IWO17_001000"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0289894.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4415..5545)
                     /locus_tag="IWO17_001001"
     CDS             complement(4415..5545)
                     /locus_tag="IWO17_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0289895.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5545..7728)
                     /locus_tag="IWO17_001002"
     CDS             complement(5545..7728)
                     /locus_tag="IWO17_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0289896.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7725..8750)
                     /locus_tag="IWO17_001003"
     CDS             complement(7725..8750)
                     /locus_tag="IWO17_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0289897.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8795..9622)
                     /locus_tag="IWO17_001004"
     CDS             complement(8795..9622)
                     /locus_tag="IWO17_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0289898.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9619..10686)
                     /locus_tag="IWO17_001005"
     CDS             complement(9619..10686)
                     /locus_tag="IWO17_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ0289899.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10712..11548)
                     /locus_tag="IWO17_001006"
     CDS             complement(10712..11548)
                     /locus_tag="IWO17_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0289900.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11545..12414)
                     /locus_tag="IWO17_001007"
     CDS             complement(11545..12414)
                     /locus_tag="IWO17_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0289901.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12484..13767)
                     /locus_tag="IWO17_001008"
     CDS             complement(12484..13767)
                     /locus_tag="IWO17_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0289902.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13781..15466)
                     /locus_tag="IWO17_001009"
     CDS             complement(13781..15466)
                     /locus_tag="IWO17_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ0289903.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15741..16766
                     /locus_tag="IWO17_001010"
     CDS             15741..16766
                     /locus_tag="IWO17_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0289904.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17361..21014)
                     /gene="rpoC"
                     /locus_tag="IWO17_001011"
     CDS             complement(17361..21014)
                     /gene="rpoC"
                     /locus_tag="IWO17_001011"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ0289905.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21093..24710)
                     /gene="rpoB"
                     /locus_tag="IWO17_001012"
     CDS             complement(21093..24710)
                     /gene="rpoB"
                     /locus_tag="IWO17_001012"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0289906.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25438..26406
                     /locus_tag="IWO17_001013"
     CDS             25438..26406
                     /locus_tag="IWO17_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0289907.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26424..27329
                     /locus_tag="IWO17_001014"
     CDS             26424..27329
                     /locus_tag="IWO17_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0289908.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27326..28132
                     /locus_tag="IWO17_001015"
     CDS             27326..28132
                     /locus_tag="IWO17_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0289909.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28289..29257
                     /locus_tag="IWO17_001016"
     CDS             28289..29257
                     /locus_tag="IWO17_001016"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ0289910.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29574..30098)
                     /locus_tag="IWO17_001017"
     CDS             complement(29574..30098)
                     /locus_tag="IWO17_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ0289911.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30385..>31485
                     /gene="scm"
                     /locus_tag="IWO17_001018"
     CDS             30385..>31485
                     /gene="scm"
                     /locus_tag="IWO17_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0289912.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTT"
BASE COUNT         8835 a         6803 c         5928 g         9919 t
ORIGIN      
        1 tcacaacctc tttttcggtc aagttcaaca acagttttta cgcgtcgatc tctggcgctt
       61 tgcccaaatg agcttggatc atccagatac gtttttctgt ggctgttttg aattcgataa
      121 agatatcttg tgttggatcg tcgccttctt ctccagaaac ttcgattcct ttttggtaaa
      181 gatctgctag ataacgatag cctgcaacaa gtgtttccaa acgttctgga atagtacgat
      241 cccagcggcc aatttcatca gggatttttg tgttatctgc aaattcacgc aatgtagaat
      301 aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc atctaactga gcgttcaaat
      361 catccatgaa ctcatccatc attgggtgca atgtaaggaa acctggtcct ctcatgtacc
      421 aatgtgtttg gtgaacaaca acagacattt ggcttagatc tgcaactaat tgatttaata
      481 cttctttggt ttgttggaat ttcatctttc ttgcctccta acattttctt acacttaaaa
      541 taatagtaga ctttcaatga aaagtacaac aaaatcattt acctgagaac gattttcagt
      601 tcgctttttt atccgtaaag ccaatcattt ttcagaatca gctaattctt ctcgcagttg
      661 agcaaattgc tgataagtct gtgctaaatg cttttgtgtc gaaacaaata atggatagat
      721 tttctgatat tttttatgat cagtttcttg tactccaact tgctctttca attctatcaa
      781 ttcctgtgct tcactgatac tttggatctt tccgatgctt ctccagccta aaatcacagc
      841 tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc gggtaaccca gtacatcagc
      901 catcatctga cgaaaaatcg gagattgagc aaacccgcct gtagcacgga tctctgtgag
      961 ctgattttcc tggcaaactc cttttaaaat ccgataaagg ttgagacaga tcccttctag
     1021 aatcgccttc atcatttctt tttctgtatg ttgccgctgt aaaccgataa aactgccaaa
     1081 cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa aaatcagacc
     1141 attggcaccc gctggcacaa aagcgatagc cgaaagcagt gcatcatatg gattttctcc
     1201 tttttcttta gcagattgaa cgacttcctg taaaaatgta tgccctgccc aatcaaagat
     1261 gccagcacca ttgctagtcg ctcctcccac tacccaatgt ttctgatcca gaacataaca
     1321 aaacgtctcc atttctggat ggaactgagg ctgagtagta ataaaacgaa gtgctccgct
     1381 cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca cctaacccta gattagaaag
     1441 cggcccgtct gcccctccca aaacaaatga tatatcattt ggcaaaccta gttctgctgc
     1501 tgtttttgat ggtagacctg ttacttgata ggtggaagga aagacttgcg gcaattgctc
     1561 tattcgaatg ttcaagtagt caagcacttc ttctgcccat tggaattcat ggatatcaaa
     1621 atatccagtt cctgaagctg ttccgtagtc gctgatcagt tcgccaaaca atctataaaa
     1681 gatatagtct ttgataccga caaaataagc agtttttttg aataattcag gttggctgtt
     1741 ttgcagccac ttgattttgt tcaatggtgt cattggatga atcggcgtcc cagtctgccg
     1801 ataaaattct ttgccaagca cggtatattt cagtttttct gcttcgtccg ctgctcggtt
     1861 atctgcccaa gtgatcattc gagataacgg tgttttctct tcatccaaca atatcaggcc
     1921 atgcatcgca gaggagaagg agacaaattg aatgattgtg tctgaaggca actgtttaat
     1981 tacttctttt ataacggtgc agacaccttg aaagatcaat tcctgatctt gttctgccat
     2041 cccttgtgtt tcttggatca aagggtagcg aaaataagcg gatgctgctt cttgggcttc
     2101 atctgtgaaa gcaactgctt tggtttgtgt ggttccgata tctaatccga ttgaaacgtt
     2161 cattttttca cctcaaattt tatgtatgta caaaaattta gtttaagctt gcgtatcttg
     2221 gttttgaaaa gggggagatg gcgtgttact ttattttatc attggttgct gtatcggatc
     2281 atttttatgt ttaaccgccc agcggattcc gttaggtcac tctattattt atcctagatc
     2341 tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa ttgattccga ttctctcgat
     2401 catcgtccaa cgatttcgct gccgctattg caggtgtcgg ttgcccttct actatttgtt
     2461 ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt actttttctt ccgcaagaaa
     2521 tttttcgacg ttcatatgga tgctttcggc tttgctattt tcgttgatgg atttatttta
     2581 cttcatggtc cattctcaca cgctattctg ttcttgggca attttatgga ttttttggtt
     2641 gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag gcggtgtgtg
     2701 ccttcgctat ggtcaatcct atctcggagc tggcgatact ctgttgatgc tttcttggag
     2761 tggtggcttg tctctagaag agttgcttca gatcttgttt cttgctagtc tgtttggaat
     2821 atccgttttt tctgtttact ttatctgtca taaaaaaaag ttggaaaagc tgcctttcat
     2881 tccttttttg agcagtgcgt tgctgattgt ccttcatctt aggtattgaa aaaaggttgt
     2941 gacaaacgac ttgtcacaac ctctgatata tagtgttctc ttaccaaatc cttgtgactt
     3001 ggagctaggc gacaggttca caacctctcc actaaatggt gttcaatcag ctgaccctca
     3061 gtattagctc aaaatgctgg ttttacaacc tctgatatac ggtgttccat aagtagcttt
     3121 ttcggcttct tatttcttga cgccccaatg tcgacgaagg aaacctgcac gtccagaacc
     3181 ttctcgataa agattgtaat ggagcttgtt tttcttgtaa taatcctgat ggtaatcttc
     3241 tgcaggataa aattcgcttc ttggttcgat ttttgtcacg attggcttat cgaatcgccc
     3301 gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt catcattata
     3361 gtagatcact ggacgataag aatcaccgcg atcagcgaac tgtcctaatg cgtcagtcgg
     3421 atcggtttgc tgccaataga tttccaccaa ctgatcataa gaaataactt ctggatcata
     3481 ttcgatctct actgcttctg tatgccctgt tgtgcctgtt gttacttgtt cataagttgg
     3541 atttggtaca tgcccgccag tataaccgga tgtcacagaa atgatccctg gctgctcttc
     3601 aaatggtttg accatacacc aaaagcatcc gcctgcgaaa actgcttttt ttgtcatata
     3661 aaatccccct tttttctcat cataccttga tttccaaaag gaggataacg ttttgcttgt
     3721 gacagaaaaa acggaaacta ttatatacgt gatacggctt ctgataaatg ccaaaacgaa
     3781 cgtacttgat caaaaacggt taaatcagga atcacatatt cagcagaagg aagatttatg
     3841 ttttcaccta ttcccaatac atgcatgtta ccagccaaag ctgcttgaca accagcttct
     3901 gaatcttcaa tgaccaagca tgcatttggc ggtacattca attgctgtgc tcctttgaga
     3961 aaaacttctg gatcaggttt tgcttttgat acttgcgtgc cgtcaataag gacatcaaaa
     4021 taacttgtca aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc tgatcccaat
     4081 ccgatcttga tgtttgtttt tttcaagtac tcaagaacag ctattgcacc aggcaaaacg
     4141 gatgtttcat ccattttttg aatcgcttga agatatagat tgtttttttg ctcagcaaag
     4201 gctgatttct ctttttctga gaatttctgt tccatttttc caaatgacag caatcgctct
     4261 agtgactcgg ttctggatat tcctttcagt tgctcgtttt ctttctcagt gaatggaatg
     4321 gacagctggt tagctaaaac cagccacgct tcgtaatgat aacgagctgt gtctacaaga
     4381 acgccatcta aatcgaatag tgctgctttg attttcatcg attacctcct tgaaaatgaa
     4441 cgacttgaag taaagatgcg atgtttttag ctgtgcgatg aagattatca gtggcttgct
     4501 gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc gaaatgccaa
     4561 catctgctgc tttttcgctt cctttaccaa cacttccgca aatagctatc acaggaacgc
     4621 cctctggtac tcttctagca atcccaactg gcgcttttcc ttttagactt tgtccatcta
     4681 gacgtccttc gcctacgatt acgaggtctg cttgctgtac ccgtttatcg aactccagta
     4741 aatccaatac gtagtcgatg ccagagacta ttttccctga agcaaatgct aacagtcctg
     4801 cacccattcc accaccagca ctactgcccg atgcctcgat tacttgaggg gcaaatctca
     4861 tataaaaatc atgcatggct tggtcagcca ctgctaattc attttctttc aagccttttt
     4921 gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa aagattagta acatcagtaa
     4981 tgacagtgat tttggcttta atcagctcaa taggcacgtt cgttttatca actgtagcaa
     5041 ttttacctag attttctccc agtgcttcta ctttttgatt atcttgatcg taaaaagcat
     5101 aacctagacc ttctgccatg ccgatgccgc catcgttggt actacttccg ccaacgccga
     5161 tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat cagttctcct actcctcgcg
     5221 tggataacct aagtggctgt tgttggttta gtgggacatc tttcaatccg acgatttctg
     5281 ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg gatttctttg tttttgccaa
     5341 atgcattagt aacgactgct gtttgccgct tcaaccttaa actgtctgct aatgatgaaa
     5401 ccgttccttc tcctccatcg cctataggta gcagatcata ttcagcctga acaaaaactt
     5461 cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc tgtcagactt tctttgaaag
     5521 aatctggcgc aatcagaata cgcattattt tcccctccgt tccaagatca gttctttttc
     5581 cagctggtat tggtctccat tgacaaaaat aggtaatgtt tctttcgtct ctttttttat
     5641 tactatttgt ttttttgtca aaacaaatag caacctgtga tttttccaaa taaaaggaaa
     5701 agaaattttt tcccatgctg aaggaagtct tggagaaata gaaagagttc cattcgttac
     5761 tgtaatacct gaaaaaccaa atataactgc tagccatagt gctcctaatg aagcagcatg
     5821 aattccgtca tctgatgaat gcggatctgg tcctaaatca atcaggcacg cttcttggaa
     5881 taaatgatac gcccattcca tttcttggat acggcaagcc tctatggcgt gaatcgcttt
     5941 actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct tagaaacgat
     6001 tggttgagaa aaatactcag gtaataagta taacagcatg acaagatctg cttgcttgct
     6061 gacttggagt tcatttactt cgctacgggc ataatcaagt aaaatagact ggctgccagg
     6121 acgttctttg taagtggtca aatcaataac tggcttactg agatacgtgt catcttgcgg
     6181 aatcagctgt tctttgttag gcactggcaa atataactta tctaaaaaat tttgacattc
     6241 tttcaaaaaa agcggatcgc cagcttgata tttaacagct aaggccacat tataatgcgc
     6301 cagatagttc gtatatgcat tattgtcgat atattctgtg tattcatctg gaccaatgac
     6361 tctgtgtatc tctaaatgat catcgacttt tacagctctg ctcagccaaa aagctgctgt
     6421 ttcatgaatg agcgtttttc cctttgtttc taaaaacgat tgatcctctg ttgcttggac
     6481 atactcttga acggcataag caaggtctgc actgatatga tgttctgcgg cagctgaagc
     6541 aacttcttgt cttgttccgg ttttgatatt caatgcagca aatttgggtg tctcttcctg
     6601 accagtgtgt gcgctttccc atgggaaaag tgcaccttga tagtgataat ctcttgcttt
     6661 ttgccgtgcg ccttctaatc gatcataacg gtacagcagc aaattctttg ctacttcagg
     6721 aaactgatat aaaaagaaag gcaggataaa aatttccgta tcccagaaaa catgcccttt
     6781 ataaccttcg ccagttagtc ccttcgcccc tacactaaca cttggatcat tgcggggagt
     6841 cataatctgt aaatggtaag cagcgaagtc catcgctaac tgatcgaaag aagaatcgcc
     6901 ttggatcgtt atccgatgct gatgccaaaa atctgtccaa taagacatcg actgctctaa
     6961 aatttctgta taactaacgc ttttctgggc ttgggcaata cttttcttaa acgggtctgt
     7021 ttgctgaaga gaggtagaga gcacgatgat cttctccagc gtaaaggacg tattttttct
     7081 gatcttttct gaaaaggaag cggtgatccg gcgatttttt gcttggaata cacctgcttt
     7141 attgaattta gctgcaatcc caatatcaat tttactttct gttgtctggt aggatgcact
     7201 gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc aactgctgtg taccaaaatt
     7261 ggtttgctgt ccatcgatac ccgttttcac atctacttgg cagtcttcgg tcaacggctt
     7321 gatgaccact ttcagggctg cctgatggag attattttga tcagcaacac gacaagtaat
     7381 gacttccact ttttttcctt gagcagtttc aatgagcgca tagcgtttaa gctctcccat
     7441 ttctaaatct agtgcccgct cgtatgcaag aactttcatc gtctcaagag aaaaatgttc
     7501 accgtcgatt tttatttccc atctgatgac atctgggaga ttcactaact cggctgattc
     7561 tgctcctaaa ggtcggttat atacaccagc tgcaaacatt ccccttactt gattccgata
     7621 gtcttcctcc tggcttcctc tgataccaaa aaatccattt ccaatggtca tctgactcgc
     7681 atacttattg atatactcta aatcaaactg tttttcttct actttcataa ggtaataact
     7741 tctcctgttt ctgccgaacg atataaattt tccaccagat tctggatgac atatccttgc
     7801 cagccatctg caatcatctg agtctggtca ccgagcgccc gattcacaaa atgagccata
     7861 cttagttcat gtgggtcatt aactggcgtg gtcttttgta aggaaaccag ctttccatct
     7921 acatcttcga atagttcgcc tggatacaga ttagcacctg cctgatcgcc acaaaaagcc
     7981 acattcagtt tgtcttctct gatattcaaa gcaaacgaag tattcagttg gatgatcttg
     8041 ccgttttcta attccagtgt gccaaaaatt gcatcttcta ctgtgaattt gcttggatcc
     8101 catttcccga atttccctga attttttttc gtaccaattt tttggaagct gtacgcccga
     8161 acgcttttta ctttaggaaa tccagtaata tacatcgctg tatcaagcat gtgaatgcca
     8221 taatcgatca aaggcccgcc tccttgaagt tctttgtcga taaacgttcc ccagcctgga
     8281 acaccagacc gacgaagcgc attcgcttct acgtaataaa tatccccgag aacatcaaga
     8341 tgattcttga tcattgttgc ttcgtcagaa aaacgatgct ggaatccata agccaaaatc
     8401 tgcccgtttt tatcggctgt ctctgccatc tttcttgctt gatcagctgt catagctggt
     8461 ggtttctcgc acatcactga agcattagct tcgagggcag ccaatacatg ttcgaagtga
     8521 aagcgattgg gtgtacagac actcacgata tcgggagaaa tctcattcag catttgtgta
     8581 actgatgtat acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc tcgctggctg
     8641 actggatcaa ccacagcggc caattctaag acatctgatc ttttttgata ataagcagca
     8701 tgtacttttt ctgctacttg tccagcgcct atgattgcta ctttcttcat ttatttctcc
     8761 ctcctttgct tatttactta ttgttattct ttctctattc tttctctaaa tcatttaaat
     8821 gacgcgctgt ttccagacag tttttcaagt attgcaatga ttcctgataa attttcactt
     8881 cattttctcc tcgcaaacga cattcatata ccatataacc ttcatactca ttttgaagta
     8941 gcgtttgaaa atggcgagta aaatcaattg cgccagatcc tggttggtag cgatgattat
     9001 ctgctaaatg gatatgaccc acatactctt tttgcgtagt caatgctttt gaaatatcat
     9061 cttcttcaat attcatatga taaaaatcag cgatgatctg cacatgtttc aattgattag
     9121 agtcaatgta atgacgcgca tccgctaaca aattgatcat atggtcttgg tatcggttca
     9181 aaggctccaa ataaataacc gtacctgttt cttcggctac tttatctaaa taacgaagtg
     9241 attcgctgac tgctttatag tctccttctt tgcttcgggg tgaaaccatc ggcggtaatc
     9301 gataagtgaa cattccccaa gctgctggga taacgattcc tttgcctcca acctcgctta
     9361 gtgcacgcaa aatctcagca atttgacgta caccattaag acgccgttct tcgataaagt
     9421 caccgatcca cccatcgtag ccgttacaag ctgtcactac tgggatccct gtttctttga
     9481 ttgcctgttt gactgtgtcg atattttcta ttaacaactt accgtcgatt tcgtattctt
     9541 caaatccaca gtctttgata aatctaaatt tttctaaaat gtctcttggg aaaaatgctt
     9601 gatcttgcgt accgaatttc attgttctcc tcctcggact tgaacgccca ttttgatact
     9661 ttcttctggg tgctggtcta cataacgcat gtaagcttct gcgctttctg caaatggcac
     9721 aactggatca atgatttctt cacaatccaa gtaaccgttc atcaataatt cccaacatgt
     9781 gtcctcgatt cgtttacggt tccatcttgg gtaatctggg tttggttcac tagatgctcg
     9841 agcaaagacg atttttgcat tattgaaatg tgcttcgcga ccaagattaa acccagctgg
     9901 aaaaggttta gcaaaggcaa cataagaaat gatcccgcca tagccgatcc ctcttaaaga
     9961 agcttgtaat gcggcagcac tgccactcgt ttcaataact gaatctactc cacgtttatc
    10021 tgtcaattcc tttagacgaa aaccgacatc tgctttcata ggatctagcg tgtaatcggc
    10081 accgtatttt tctgctaatt ctctacgctg cgctatggga tcgatcccaa cgatggtagt
    10141 agcacctgct tttttagcta attgaatcgc aatttgcccg atagcgccta aaccgaagat
    10201 tgctacagca tcacctgctc gaacatgtgc ttcccggata ccacctaaag cgaattgagc
    10261 aggatcgtaa catactgcat ttttccattg atccaaccga tcaagtttac gtagtttata
    10321 attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc agactaactc
    10381 tcctttttga tactctgtca ccaaactgcc cgtttcttcg atcgtaccga caatcatatt
    10441 tcctaattga aattcgccaa ataccacacc gcgttggctt ccttcttctc ttggcaagaa
    10501 taggcgccat tcttctgaaa attcttcatc gataaatgga cttaatcctc taaagtcgac
    10561 aatttctgtt cctggttttg gagtagcgaa aattactcgg atacgtactt cgttcttttc
    10621 gatctcacgg tcttcgtaat ctttgaatcc ggctattctt ggttctaatg caactaattt
    10681 tttcatcttt tctttcctct ttcttttctt tttatccttt tacgccgcct tcgactaaat
    10741 cacctttaat aaatcgttca gataacgcat acataatgat gactggtaaa gcggtcacca
    10801 atgaagcggc catcattcgt ccccaaatgt agtctggtgt actgaataat gtgttcaaac
    10861 cgattggtaa agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat aaataatcat
    10921 tccacgaaac cataaagcaa taaacgaaga cagaaatgat tccagaaata gccaatggta
    10981 taatgatccg aataatgatc tggatccggc taagaccatc gatcattgct gcttcttcaa
    11041 tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa tatcgcagta ggaagtgttt
    11101 gaacgaccat cgtgataatc agtgctgtat tggtatcata caaacctaga ccggaaatga
    11161 ttttaaataa tggaacaacg atcaaaatac cggaaaacat ataaacggtg taaaagctag
    11221 cattgatttt cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc ccgctgatcc
    11281 caatcactac tgcagttacg gaggctatca aggagatcac taaactattt ttgaaataca
    11341 tgatgaatgg aaaaatctca gcattgaaaa tatcctgata atgttcaaaa gtccattctt
    11401 tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga atccttgaat gatgtcatca
    11461 gcatgatcaa aaatggaaac aaattgacag ctaatagcaa caaaatcgtc gcgtaaaata
    11521 agattttcct gctcttttta tttttcattt caagtccacc ttctttcgtg agagcaagac
    11581 gaccgctgca ataatgatga acaatagaat cgatatagct gctgcttttc caagatcatt
    11641 gaacgcaaag gctgtctgat acaaataaac gcctaagata ttgactttat tcgtcagtag
    11701 atagacatct gtaaacatat agaacatcca gatagatcgt aaaacaatca cagttgctag
    11761 tactggtttg attgccggca gtgtcacgat cttgaacttc tgccaagcat ttgcaccgtc
    11821 catttcagct gcttcataaa gcgttttgtc gactgtttgc aaaatagcca aaaatgaaat
    11881 aaacgcatac ggatagtatc tccagatagc aaacaaaacg accataaaga agctagcaac
    11941 tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta ctaacagatc
    12001 attgacgatt ccataactat tattgaacat atatttccaa gcaaaaatca aagaaataga
    12061 tggcgtaaca taacttaata aaatcagtga acgcaccgtt ttcctaaatc tgaacttgcg
    12121 gttaagcaaa atagctacgg ctaatcctaa agctgtactg cctgctacaa ctaagaatgt
    12181 gtaagcaact gttaaaccta aagaacgata aaattcacta tcacttaaaa tttcaagata
    12241 attgcttaat ccgataaaaa cggcgtctga gttaggattc agcggcatat cgaaaaaact
    12301 gatctgaata ttggaaagca ttggaatcag cactaaaaca gctaataaaa gcatgcttgg
    12361 tgctaaaagt cccatggcta attttaagtc agaatgtttc cttggacgat tcattgaaac
    12421 ctccccctga taatctgcca atagctatct acttttttcg aacaccattg gcagaacttc
    12481 ttgttatttc gtattttttt gtgcgtcagc taaagcttga tcgacatctt cgtttccgac
    12541 agttacttgg ttcactgctt ttccgatcac acctgagctt gtgatatctc ccattttcgt
    12601 gaaattcttt tccccgacta gtccaaagac ttgtacttca tcaaaagcac tagcaatctc
    12661 agatggcagc tcgccaaaag acttgatgac ttcattttct ttgtaaccat ccagttcgac
    12721 tacttgttta ttcactggtt gagcaccacc aggagacatc aagacccact tttccatgtt
    12781 tttcggttga gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt tctcgtctag
    12841 accagaagaa atcgtcaatc cagatactgt gccatagaca gcttgatctt tttgtgttgg
    12901 gatagcaaaa ccgatatttt ctgagttccc cgcttcataa acagaaggca aaatgtaagt
    12961 tgaatagatt gccattggtg ctgttccatt catgaaagca tctttgattt ctgtcacatc
    13021 attagagcct ggcatcgtgt attgggataa gttttgataa aacgacaatg cttctctcat
    13081 cttttccgta tcgatcgtta cctcgccttc atcatccaaa acatttgctc catttgacaa
    13141 agcaaattga gagaacgctt gctcactcat tgttccttca gcagtaggca tagcaattcc
    13201 atattgttta ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat catcccaatt
    13261 ttctggttct gaaaaaccag cttctgataa tttttctttg tcataccaaa taccttgaac
    13321 ccagccgcta attggtgcag caatatagct ttttccatct tctgaacgaa caaggttttt
    13381 ggctccgtca taataattat cttctccagc ttcctcgata acgttttgga ctgccgtctg
    13441 atcaatcagt tcatctttat ccatcacttt agcaaaatcc tgactgactt ccattacttc
    13501 tggtaattta ccggaacgag ctaacgtaac gatttttgta ttgaacgctg attcttctac
    13561 cggcacttgc ttgattttga tgtctggatt ttctttttca aaatcagcaa ccagttcatt
    13621 gatcacactt aatcgttcct gttcgacaga agagtgcatg aattgaatcg ttgtctgttg
    13681 tgattcgtta gagctttgac tttcaccaca agcagtaaga agaaatccag tagctagcag
    13741 acaggtagta acaataattt ttttcatttt tttccacctc ttattttatt attttcagcc
    13801 acatatattc atacggctgg aatatcaact ttcgttcttg actgcaatgg ataatttgtc
    13861 cagaaaacag gtctaggtaa tctccttttg gtaaaagata acctgctgat tgattggaaa
    13921 aattatggat aactaagact ttctcttcct tcgcttcgcg aataaaactg ataaaagaat
    13981 ctgctgagtc cagtaagtaa atcaaactat tcggatggaa ggctttttct ttttttcgaa
    14041 tagatagcat ctgggtcaat tcaccatata ctgctgttct taatggatct gtctgcaact
    14101 ctttctcaat atctgctaac aaaaattttt gccgattgat ttttcgattc tctcctgttt
    14161 tttctacgcc tttgtaatca tttcgacttc ccagcacact ttgaatataa attgccggca
    14221 cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt ctccattccg
    14281 tcgtatctgt tggagaactt aacgcatcta gatacgtagc gtttatctca tatggactgg
    14341 tcgttccatc tgggtttttc ttgtagttta ctcgggtacc ctctttcttc agctgttcca
    14401 ctaattcaag gatcgttttt tccggcagaa tacctcgtac cggattcaat ccgataccgt
    14461 catgggaggc taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg atggttttta
    14521 accattcttt caacttactt gtattacccg aacgaatcgc ttgaagggtc aaaggtggta
    14581 gcgaaaactg atagaccatc tgtgcttcat tctcgccatt cccaaaataa ctgatattat
    14641 ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc tggagctgct tcatcagcga
    14701 ttgcacggaa taattggatg attttatgcg tttctggcag gtgtatacaa gaagttccta
    14761 gttctttcca taaaaatccc acagcgtcca agcgaataaa tgaagcgcct ttttctaagt
    14821 aaaacaaaag aatatcgatc attttcaata aaacttctgg atttttaaaa ttcaagtcta
    14881 cttgatcttc gctaaaagtc gtccatacgt gtttttcttt cccgtttttg tcaatgaacg
    14941 ttgtaagtaa cgggctggtc cttggtctag tcacgctgct tgtatctgta tccagtggca
    15001 aatcaataaa aaaatctttg tacgtatcat tcaagtttaa atattcctga aaccaagtac
    15061 tttcggcaga catatgattg cagacaaagt cgaacattaa ccgtgtttcg cttctcattt
    15121 cttctatatc ttcccatgtg cccaaacggt cgttgattgc ctgataattt acaacggaga
    15181 aaccatcatc agatgtatat gggaaaaatg gcaagaaatg tacaactcta aatgtatctg
    15241 ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc tcttcaaaaa
    15301 attgatcccc ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt ttgcctgcct
    15361 ggcctttctt ctgctgataa acactgactt tttgttcaat catagaggcg gttttctcag
    15421 acaaatctga gtcgcgataa atcgcttgta aaagttttgt atacatcgga ttcccctttc
    15481 ttttttggga gcgttcccaa cgatttgatc atattatgga aaacgcttac tgtcaatagc
    15541 atttttttaa ataattttga tatacacaaa aatacaaata agaaagctta tgtatcaata
    15601 attaatagct tcttaaaaaa atatggtctc tcatgatcat gcaagctatt cctaaatttt
    15661 caaaatcata gacaaaaaag aatgtctatt gtactatgga attattctaa aacgctccca
    15721 aaaaggaggt gcatataaaa ttgaaaccta ctatttatga cattgcgaaa aaagctaatg
    15781 tctcaaaatc aactgtctcc agagtattaa ataaccagcc aaatatctct gataaagcaa
    15841 aagcaagtgt acttcaagca atcaaagaat tgaattatga accgagcaaa attgcaagag
    15901 gactgtcagg aaatggtttt gacgcgattc tagcattatc tcatcgtact tctcatacga
    15961 cctatggcaa tccttttttt tcagatgtta ttcaatccat ttctgccgtt tctgaacaaa
    16021 ataacttcga cttgattcta caaacggcaa aaacaccaga agaagagcta gaaaaatgcg
    16081 cgaaaaagat ccaagaaaaa atgatccgag gaattattat tttaagctct acgatggatg
    16141 aaacacttct ttttgaattg gatcaataca atattcctat tgtggtaatc ggaaaagtcg
    16201 gaaacgacta cacgaacatc tactcagtgg acacggataa tttccaggat agttacgatt
    16261 tggtggaaac actgatcaag aatggacacc gtcatatcgg ctgtctccat gctccgacag
    16321 aagtttatgt atccaatgat cgtctcaatg gttaccgtca atgcttattt gatcaccacc
    16381 tagatatccg taatgaatgg attatagacg gcggctttcg cttagaagat agcatggcag
    16441 caatgaatga tttaatgaag ctacgagaaa gaccaactgc gattttttct accgatgcat
    16501 taaaatcatt gagtatttac aaagtggcag aacaatctgg aaagcagata cctgatgatc
    16561 tttcgatcgc aggattcagt gatcccttat tgtcctccct attttcgcca cagttgactc
    16621 ggatagacat tccgacaaat cagttaggaa tcaaagccac agaactttta ttccaactaa
    16681 tccaccacca ataccctgaa gaaaaaagaa tcattctttc aaccgagatt actctcacag
    16741 attcgatcca acaacaagaa atgtagaaca gaattataaa agaacagaaa cactgaggat
    16801 aagtcagtta ctctgttctt ttttcaatca ctcatccctc gttaccccct cccggtattt
    16861 tagcgaattt ttcttatttt ccatctgttt tagatagctg tggataacta taatgtttgt
    16921 gttaaactat tttttccaca gggaagaaaa aactattttt aaccacgttg tgtataagtt
    16981 tttatagttc gtttttttaa acacatttac ccttatttca atccatgtta tttggggata
    17041 cttatctcta aaacataaat agaataacag acatgtctac tggttttgtt ttattcgagg
    17101 aattattcaa taatagctag caaaatatta aaaataaccg ctctatcttc ccatcaattt
    17161 attttgcatt caaaaaatga tctatcttta cttttgttga ttgacttctc ttggaagatg
    17221 atcgttccga taccttaatt ggatacgaac cgatgttgtt aagatccttt cccacttttc
    17281 ttctagttag acaaattatt ttagacaaac caaaaaagag gttgtgacaa taatttgtca
    17341 caacctcttt tttaaaatat ctattttgtt tgattgttca ttgcgtcttc tgcagccatt
    17401 tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg ctacaccgac ttcttttggt
    17461 tccatgtcac gataacgttg cataccagta ccagctggga tgatcttacc tataataaca
    17521 ttttctttca aaccaagaag aggatctttc ttaccgcgga tcgcagcatc cgtcaagaca
    17581 cgagttgttt cttggaatga tgcagcagat aagaaactat ttgtttctaa tgatgctttt
    17641 gtgataccta gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac tagcgtattg
    17701 tagttttgat ctttaaagtc tgcgatgtct aataatgtac ctggtaagat atctgtatca
    17761 cctggatcca ttacacgaac tttacgcagc atttgacgaa ccattacttc gatatgcttg
    17821 tcgccgattt ctaccccttg catacggtaa acacgttgta cttcacgaag caagtagttt
    17881 tcaacagata atacatcgcg aacttgcaac aattgttttg gatcgattga tccttctgtc
    17941 aatggtgcac cacggtggat catatcgcct tctgctactt tcatacgtgc tgtataagga
    18001 acagtatacg tacgtgtatc tgtttttcct ttgattgtta cttctttggt acgtgttgca
    18061 ggatcttctg agatatcgat gacatcccct gttacttctg tgatcactgc ttgccctttc
    18121 ggattacgtg cttcaaagat ttcttggaca cgaggaagac cttgagtgat atcgtctccg
    18181 gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag taccaggttc accgattgat
    18241 tgagcggcga tcgtaccaac tgcttctccg acttcaacgt cagagccagt tgcaaggttg
    18301 cgtccgtaac aatgtttgca gacaccatgt tttgtattac atgtgaagac agaacggatt
    18361 gtcactgttt cgacacctgc atcaacgatt tgttttgcga tgtcttcgga aatcaattgg
    18421 tttcctgcaa tgatcaattc atttgtttca ggatgacgta cttctttacg ggtgtaacgt
    18481 cccactaaac gatcttctaa tgattcgatg atttcatttc cctcgcggat ggcttggatt
    18541 tcaagacctc ggtcagtacc gcaatcttct tcacggatga ttacatcttg ggccacgtca
    18601 accagacgac gagtcaagta acctgaatcg gcagtcttca aggctgtatc ggtcatccct
    18661 ttacgagccc cgtgagttga gataaacatt tccaagacgg atagtccttc acggaagttc
    18721 gaaatgattg gcaattccat gattcgacca ttaggagcgg ccatcaaacc acgcattccg
    18781 gcaagctgtg taaagttgga aatgttacca cgagctccag aatcagacat catgaagatt
    18841 ggatttttcg cttccaaacc ttccatcagt ttttgttgga tgctgtcttt tgcagcattc
    18901 caaacagcaa tgacacgttc ataacgttcg tcatctgtga tcagaccacg acggaattgt
    18961 tttgtaatag tttcaacttg tttatgggca ttttcaatga tttcttgttt ttcattcagt
    19021 acactgatgt cagcgatccc tactgtgata ccggcatgtg tcgaatgttt gtaccctaag
    19081 tctttcatac ggtcaagcat cttagaagtt tctgttactt tgaatcgttt gaagacttct
    19141 gcgatgatgt ttcctaagtt tttcttcttg aacggtaata ctaattcttg ctctttgata
    19201 tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt ccggtgtttg gacggtcaag
    19261 ttgaagtctg ttggttcatt cagataaggg aattcaggag gcatgatctc gttgaagatg
    19321 atcttaccaa ctgttgtgat catcgtacgt tctttttgcc attcagtaaa tggtttttca
    19381 cctaatgagt tagggttcac accgatacgt gaatgtaaat gaacatatcc gttgcgccat
    19441 gcgataacgg cttcgttttg atctctgaag accattcctt cgccttcgcg tccttcttct
    19501 tccattgtca agtagtagtt acctagaacc atatcttggg aaggagtaac aactggttta
    19561 ccatctttag ggttcaagat gttttgagca gctaacatca acatacgtgc ttctgcttgt
    19621 gcttcttcat tcaatggaac gtggacagcc atttggtctc catcgaaatc ggcattataa
    19681 gcttcacata caagtggatg caaacggatt gcgcgtcctt gtactaaaac tggttcgaat
    19741 gcttggatac caagtcggtg aagcgtaggt gcccggttca gcaatactgg gtgttccttg
    19801 attacgtctt ctaaaacatc ccatacttcg tcttcttgac gttcgatttt acgtttcgca
    19861 tttttgatgt ttgaagctag ttcgcgttga acaagctcac gcatcacgaa tggtttgaat
    19921 aattcaatgg ccatttcttt tggaagtcca cattggtaca tcttcaagaa aggtccaaca
    19981 acgataacgg aacgacctga gtagtcgaca cgtttaccta gcaagttttg acggaaacga
    20041 ccttgtttcc ctttcaacat atgagaaaga gatttcaatg ggcggttacc tggtccagta
    20101 actggacgtc cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg cagcatacgt
    20161 ttttcatttt gaacgatgat tccaggtgca ttcaagtcta acaaacgttt caaacggtta
    20221 ttacggttga ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa acgtccacct
    20281 tctaattgaa ccattgggcg taaatctggt ggaataacag ggataacgtc cataaccatc
    20341 cagcttggtt gatttcctga agcgcggaat gcttctaaaa tatccaaacg gcggattgca
    20401 cgtgtacgtt tttgtccttg tgcagatttc aattcttctt tcaattcggc tgcttctgca
    20461 tctagatcaa cattatcaag taattgttta atcgcttctg cacccatagc agcttggaat
    20521 tcttgtccat attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa ttgttttttc
    20581 tctaaattcg tatttcctgg ttcgatgact acatatgaag caaaataaat gatttcttcc
    20641 aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg aaggaatccc tttaaagtac
    20701 cagatatgtg aaacaggagc tgctaattca atatgtccca tgcgttcacg gcgaactttc
    20761 gaacgagtaa cttccactcc gcatcgatca caaacgattc ctttataacg gatacgtttg
    20821 tattttccgc aggcacattc ccagtctttt gtaggaccaa aaatccgctc acagaacaac
    20881 ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct caggtttttt tacttcaccg
    20941 taagaccagc ttctgatttt ttcgggagaa gccaaaccta tttgcatact ttcgaattta
    21001 tttacatcga tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc aagacggctg
    21061 atcttcgacc gccttgcagc tcatagaccg gattagtcta aattatcttc tgcggtttca
    21121 aagttttgga tgacatcgtc tttttcatct tccacttgtt cagcagcttt cttttctaat
    21181 tctttggcag tttgctgttc ggcaaatttt gtcaaagcat caacagtgat caaatcgtcg
    21241 tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa gacacgcatg
    21301 tccagaccta gtgattgcaa ttctttcacc aatacgcgga atgattccgg tacacctggt
    21361 tttggaattg gttcaccctt gacgattgct tcgtatgttt tcacacgtcc gactacgtca
    21421 tctgatttgt atgtcaagat ttcttgtaaa gtataagccg caccgtaagc ttccagtgcc
    21481 catacttcca tttcaccaaa acgttgtcca ccaaattgag ctttacctcc caatggttgt
    21541 tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca atttgtcatc gaccatgtga
    21601 gcaagtttga tcatgtacat cacaccgaca gacacacgtc catcaaatgg ttcacctgta
    21661 cgtccgtcgt acagaacagt tttcgcatcg ctggccatac cggcttcacg aactgtttcc
    21721 catacgtctt catcacttgc accatcaaat actggcgttg ctacgtggat acctaactga
    21781 cgtgcagcca tacccaagtg taattccaat acttgcccga tgttcatacg tgaaggcacc
    21841 cctaaaggat tcagcatgat atcgattggt gttccatcag gtaggaatgg catatcttct
    21901 tccggcataa tacgggatac aaccccttta tttccgtgac gaccggccat tttatcccct
    21961 tcatggattt tacgtttttg aacgatataa acacgaacta acatgtttac gcctggtgac
    22021 aattcatctc cagcttcacg agtaaagatt ttcacatcgt gaacgatacc gccgccgcca
    22081 tgtggtacac gtaatgatgt atcgcggact tcacgagctt tttcgccaaa gatcgcatgc
    22141 aatagacgtt cttctgcaga taattctgtt accccttttg gcgtgacttt ccctacaagc
    22201 aaatcgccat cttggacttc tgcaccaata cggatgatac ccatttcgtc taaatctttt
    22261 aatgcgtctt ccccgacatt agggatttcg cgtgtgattt cttcaggtcc taattttgta
    22321 tcgcgggctt ctgattcata ttcttcaata tggatagaag tgtatacgtc atcttttacc
    22381 aaacgacggc tcatgatgat cgcatcttca tagttgtacc cttcccaagt catgaaacca
    22441 actaaaacgt tttggcctag agccatttcg ccttgttcca tagatggtcc gtctgccaga
    22501 gtatcccctt tttcgacttt ctcacccaaa tgaacgattg gacgttggtt gtagcttgtt
    22561 ccggagtttg aacgacggaa ttttgttaca tcgtatttat ctaatgcacc gttgtcgcgg
    22621 cgtacgcgga tttcagaagc atcaacgtat tcaacgacac cgtcatgttt acataacaaa
    22681 gcagctcctg agtcatgcgc tgatttgtat tccataccag taccaaccca tggtgcttga
    22741 ggattgatca acggcactgc ttgacgctgc atgttggcac ccatcaaggc acggttggag
    22801 tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg caactacttg tttaggtgat
    22861 acgtccatgt agtctacttt atcaatagat acttctaagt tttcactttg agcacgagcc
    22921 ataacaacgt cttgagcaaa tgttccatct tcattcaatg ggctgtttgc ttgggctacg
    22981 atgtaatggt cttcgatatc agctgtcaag tagtcgattt ggtcagtaac acgacctgtt
    23041 tcacggtcta cacgacgata aggtgtttcg ataaatccga atttatttac tttcgcataa
    23101 ctggacaaac tattgatcaa cccgatgttt gggccttcag gagtttcgat cggacacata
    23161 cggccatagt gggagtagtg aacgtcacga acttcataac cggcgcggtc acgagtcaat
    23221 ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca attcgccaag agggtttgtt
    23281 tgatccatga actgtgacaa ctgagaagaa ccaaagaatt ctttgatact tgccacaact
    23341 ggacggatat tgatcaattg ttgaggtgtc aatgtttcag tatcttggat cgacattctt
    23401 tcacgaacca cacgttccat acgtgctagc ccgatacgga attggttttg taaaagttca
    23461 ccgactgaac ggatacgacg atttcctaag tggtcgatat catcgacatt gccgatacct
    23521 tccatcaagt tcaagaaata gctcattgaa gcaatgatgt cggctggacg aaccgttttt
    23581 acagctgctt ctggatagcc gtttccgatc acgtttactt cacgttcagg gtctcttggt
    23641 gaaaatactt tgatgacttg gacagtcatt ggatctgtga ccacaccgtc ttctgatgga
    23701 tagtaagtca ctgaatttag gccattatca atgaaaggag ctagtgtttc catgacttgg
    23761 tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg tttctggatc aactaatgtt
    23821 tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta attttttgtt tactttgtaa
    23881 cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt cagcaagtta
    23941 cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac gtctttcaaa
    24001 ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac tgtattgcgt
    24061 aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa tcctaacgca
    24121 cgaactaata cggttaaagg aatcttacgt gtacggtcga tacgtacata agagatgtct
    24181 tttgcatcag tttccatttc tagccaagct ccacggttag ggataacagt agaaccgaat
    24241 ccttcttttc cgtttttgtc gacttttcca tggaaataga cacctggtga acggactaac
    24301 tgagaaacga ttactcgctc tgccccgttg atgatgaatg ttccttgttc tgtcattaat
    24361 gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg gtttgtcaaa
    24421 cgcaatgtta catgtaatgg tgcagagtag tttgcatcat gcgcacgagc ttctgctact
    24481 gtatactttg gttctttcaa ttcataatct acaaattcca aagatagatt gccattgaag
    24541 tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa gaaccattgg
    24601 taagagtctg tttgaatttc gatcaaattt ggtaattcca acacttcact gattcttgcg
    24661 aaacttctac gttcgcgatg ttttccgtat tttactacgt gtccagccaa gctcttcacc
    24721 cctctaaaat gtcattcaaa aagactaccc gatcaattgt tacaataata tttcaaatat
    24781 tatttttagg taacaacaga cattttttgc ggcttttagg caaaaaaaaa ccaaaaatag
    24841 aacattctct tgaaacctta cttcgtgagc ttctactttt gtttccttat attaaagccg
    24901 aaacggacaa tatttcgttt ctgccttttc ttcatcagat agtttttgca acttcatata
    24961 ctacacttat tttctataaa agtcaacact tcctggcgtt tcttctgctt tttcgcaaca
    25021 ttttcttaat gttttcaatt ttatgttttc aattatctga caattcttaa ttgacagacc
    25081 tttttatctc ggttatactt tagaaaattc attttgtgga ggaacttcta tgcgaaccaa
    25141 catcaaccaa atcgcaccaa cacaactgaa taacttttac tttagctact ttagataggt
    25201 ttgtattcat catgatggat acaaacgtta aacgacagtt tgtatctatg atggctaaag
    25261 aactttgtat tgcagcaaaa agccacgtag acaaaatgct aagtggctga gcagtgaaaa
    25321 tacaaagtaa aagtatcaag cattctactt tttcaattta ttttccgact tttcaatcca
    25381 tttggacatt ttgtctgaat ggattttttg tttcccaaaa ttaaggagga aaaagaaatg
    25441 aaaaaaaata taatcatcgg attaggcgct gccctgctac tagctttagg aggttgcgca
    25501 caaaaagaca attccgcaga ggcatcagat ggacaaaaaa caattggcgt attgcaactt
    25561 gtacaacata actcgttaga tgctgcctat aaaggattca aagaaggatt agcagaagaa
    25621 gggtataaag aaggcgataa tctaacaatc gattatcaaa atgcccaaaa caatcaagac
    25681 aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc ctgatttgct acttggaatc
    25741 gccactccag cagcacaatc tttattaaat gaaacaacca ctatcccgat gactgtcaca
    25801 gcggtcacag acttggaaca agcccattta gtcacatcca atgaaaaacc tggcggcaat
    25861 gtgactggca cgacagatat ggtgccaatc gaaaaacaaa tcggcttact gttaaatatc
    25921 gtgccagacg ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa ctctaagatc
    25981 caagctgatt tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa agtactgaca
    26041 gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga tgtagatgga
    26101 atctatattc ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg tgaagtcgca
    26161 aaagaaacaa aaacacctgt cgttgctggc gcagttgatc aagtcaaaga cggcggtcta
    26221 gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa cagggatcat ggcagcaaaa
    26281 atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga tttggaactg
    26341 tatgtcaata aagacatggc caaagcatta ggcatcaatc cagacagcat caaagaagct
    26401 gaataaaaca caggaggatt ctcatgacaa atttgctctc tcttttctta tcatctgcat
    26461 cgcaaggggt cttatgggcc ctacttgcga ttggtgtata cgtcactttc cgtatcttgg
    26521 acattgccga cttgactgct gaaggaagct ttcctttagg tgcagggatc gcggcagtaa
    26581 gtattacaaa tggctatcat ccattgacag cttgtcttct aggcttttta gggggtgcgg
    26641 cagcagggct tgtttccgga ctgcttcaca caaaattgaa aattcccgcc cttcttgcag
    26701 ggatcatcac catgactggt ttatattctg tcacatcccg tgtgatgggt gcgccgaaca
    26761 tctctttatt gggtcagcca acgatctttc attgggtcga gtcattcggc ataacaaaag
    26821 acaacgctgt actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt ttacttgttt
    26881 tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac tggcgataat ctggcaatga
    26941 gcgaagcaaa cgggatcaac acagataata tgaagatcat cggttatatg atttctaatg
    27001 gatgcattgc tctttctggc tccttgcttg cgcaaaacaa cggctttgct gatttaaatt
    27061 ctggtattgg aacgatcgta attggacttg cttctattat catcgcagaa gttttgttac
    27121 gtaaccagcc actatttctc cgtctcttga ctgtcgtttt aggtgcggtc atttatcgtt
    27181 ttattttagc gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa cttgcttctg
    27241 ctttcgtgct agtgatctgt ctttctttcc cgcagatcca gcaaaaattc catttacctc
    27301 aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta acattggaaa atctccatca
    27361 tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta atctttcctt
    27421 agatcccggc gacttcgtga cgatcatcgg tggaaatggg gctggtaaat ctactttgct
    27481 gaacagcatc gccggtacat tttctgtcga tcagggtcaa attttattga atggaaaaaa
    27541 tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat tccaagatcc
    27601 taaactaggg acagccgttc gtttaacggt agaagaaaat cttgctcttg ctatgaaacg
    27661 aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa gaccgaacat ttttcaaaga
    27721 ccagctggtt cggctaaatt tggggttgga aaatcgttta tcctcagaaa tcggtttgct
    27781 gtctggagga caacgtcaag ctattacgtt attgatggcc acattacagc gtccagaact
    27841 gatcttatta gatgaacaca ctgcagcgct tgaccctgct acttccaaaa cggtgatgga
    27901 attgacagaa aagctgatca ctgagcagaa tctcactgct tttatggtca ctcatgacat
    27961 ggaagacgcg atccgctatg ggagtcgact gatcatgctg catcaaggaa ggatcgtcgt
    28021 agatatccag caagaggaaa aacagcagct cacagtgccc caattattag cgttgttcaa
    28081 acaaaacagc ggcacggcat taaaagacga tcaagtactt ttatcttcat aatcagaaag
    28141 aggatgggac aaaaaatgaa cgcccaccct cttctttttt ctctttcctt ctttcgtttt
    28201 tgctttatgt atcaaccgtt gtttcttcga aaactatttt gttctctcct tcttcctgat
    28261 agaatggatg ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac ttcaattatt
    28321 gaaaaagaca ccagattttc tttccggaga aaagttggca cagcaactgg aagtttcccg
    28381 gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt ttgagcacgg
    28441 gcctacaggg tatcgttacc tgccctctga cgtactagat gcaaaagaaa tccagcaaga
    28501 gctacggtcg tttattcctg atttgaaagt acaaacgatg ttgacttctg aatctacgat
    28561 gaaagatgcc aaactggcag cagctcaagg agattctgtt tcgaagttat ttgttgctga
    28621 tacacaagaa caagctcgtg gacgttttgg acggcctttt ttcgctgaac ctggacgagg
    28681 aatctacatg agtctcttgc tccatccaga caaacggttt gatgaattgc ctcaatatac
    28741 gatcatcgca gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc gttctgcttc
    28801 gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa gtctgtggga ttttatctga
    28861 agcagtcagt gatatggaaa gcggcaggat cagtcatatc atcattggga tcgggcttaa
    28921 tttttctgtt cctcaaaatc attttccagc tgatctccaa gaaaaagcca tctctgtctt
    28981 tcccgacgga caagctagtg tcacaaggaa tcagctaatc agcaaaatct ggcaagaatt
    29041 gttcacttat ctttctcacc cagaaaaagc gttggacttc tataaagaaa aatcattcgt
    29101 attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc ttgcaaaaga
    29161 aatcggtgat catggcgaac tgatcgtcca aacaaatgaa cagaagatga gtttgtcttc
    29221 tggcgaaatc agtctcacat ctatccgtac gcaataatta tcagatttca caaaaggaca
    29281 cctgatctca tacagtgtcc ttttttctga attctcttca aaagactctc cttattctat
    29341 cagctgacag tttctctatc ctctagaaaa aggtattcta aaaatatcta taaagcatgg
    29401 tacactttct ttaaatgaat caatgcaaaa aacaagagag tcatcagttt acacgatcaa
    29461 aagagatagc aggccgcttc ctttttcaat cttatagata gaccatgggt aacgataaaa
    29521 acaaaaaaga acttgcgact tattttgccg taagttcttt tttagctagt gccttaatct
    29581 cttaatccca acaaacgtct cgcatagatc acctgaccag agtgcatagc agcatcatca
    29641 ataatagaaa cgagacgcat tcctcgtgtg actggtggtg tccagttctc gtcaattact
    29701 tcatctagct cggaaggttc cagccttctt aagtaagaaa tagcaaaatc agcagctcct
    29761 cgcaagtatc ctagcagatc ttctatttga ttagcttgga tctgctttgc ttgttccaga
    29821 ctatgcttcc aatcttgttc gttatctgct atcgtcatcg gaaaccggct ttcccagtct
    29881 tgactatgcc aaattggaga agttcctgct aaatcagcaa tctggaaatc taattctcga
    29941 gcggtatgcc aagccagcca agtcattgat ttgatctgcg gggctctatc ttccactgga
    30001 aaacgattgg cctcctcaat agagacgcct tcaaacaaac gttcaaaccg atcgtatgcc
    30061 cgatccacac tctcgcatag catatctaaa taatccattt attttcctcc tttttattta
    30121 tcctacttac tttttcctaa ttctaggata cctacttata tgttactaca cacccttatt
    30181 ttttacactt taacagtcac catcatcaat agtttggaga ttgaaagaaa aagatagtgt
    30241 cgaactatca attgaacaag aagcaacaat atgaacgttt cattaatatt ctttaaataa
    30301 acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa taggaaaatg
    30361 tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt cctagttgct
    30421 ttgttgttca atatcttttc gtatggtgtg gccgtttcag cggaaacata cattcctaaa
    30481 gaccctggga atatttataa tgatctaact ggtaactatg gcttgttagg aattgctagt
    30541 caattccacg tatttgctaa aaataagaca actgtgaatg cacatacaga cggaaatata
    30601 gcaacacatg aacttgatgc aaaaaataat tttggtacag agcttcatga aggtttattg
    30661 aaccaagaaa taaattatat acaaaaaact gattctttcg ctaattcttc aggcatacct
    30721 acaggtagca caagaatgaa taaatttgtc gttggcagta acgtggatgt agcacttcaa
    30781 gatggtcatc ctacagtcaa tggatccagg cttgatcact taactccaga tgatttttat
    30841 aaagatcgtg ctgggcatga atatattgat ttcgagcagg aatttgctaa attgaatgta
    30901 gcagcaaatg atcttgcaac gatcactcca gctaaaacat atactgctgc agattttcca
    30961 gacatgaaca atcggacaat cgatttaact gatttatcag ataccgggtt tttattggta
    31021 aatatcgatg cagaagtgtt aacgatgaat acgccacttc aaatcattaa tccaaatgat
    31081 caagtagttg tatttaatgt catcaacagt tcttctgcat tgaacgtaca gtcaccaatc
    31141 aaatacaatg atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa tatctcttgg
    31201 aattttggaa atgagatgac tgatttgaca atcagtgctc cttttcaagg aacaatcctt
    31261 gcgcctaatg ctactatccg cattactcaa aaccaagacg gaacgattat aggaacaaat
    31321 gtgattttag atgcagctac caatcgttgg gatcctaacg aaatatttat cagcgacaat
    31381 ggtacagata cgacagacac aagcgacagt acagatacat ctaccacaag tgatagctca
    31441 gattcatcaa cttcaagcga cagtacagat actacctcta ccaca
//
LOCUS       DACECR010000024        30904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.024, whole genome shotgun sequence.
ACCESSION   DACECR010000024 DACECR010000000
VERSION     DACECR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.024"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(297..3575)
                     /locus_tag="IWO17_001019"
     CDS             complement(297..3575)
                     /locus_tag="IWO17_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ0289913.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3846..4391)
                     /locus_tag="IWO17_001020"
     CDS             complement(3846..4391)
                     /locus_tag="IWO17_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0289914.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(4403..5095)
                     /locus_tag="IWO17_001021"
     CDS             complement(4403..5095)
                     /locus_tag="IWO17_001021"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ0289915.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(5114..5398)
                     /locus_tag="IWO17_001022"
     CDS             complement(5114..5398)
                     /locus_tag="IWO17_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289916.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(5411..5974)
                     /locus_tag="IWO17_001023"
     CDS             complement(5411..5974)
                     /locus_tag="IWO17_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0289917.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            6202..6864
                     /locus_tag="IWO17_001024"
     CDS             6202..6864
                     /locus_tag="IWO17_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ0289918.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            6988..8184
                     /locus_tag="IWO17_001025"
     CDS             6988..8184
                     /locus_tag="IWO17_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ0289919.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(8285..9661)
                     /locus_tag="IWO17_001026"
     CDS             complement(8285..9661)
                     /locus_tag="IWO17_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0289920.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(9698..10564)
                     /locus_tag="IWO17_001027"
     CDS             complement(9698..10564)
                     /locus_tag="IWO17_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ0289921.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(10653..11678)
                     /locus_tag="IWO17_001028"
     CDS             complement(10653..11678)
                     /locus_tag="IWO17_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0289922.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(11696..12370)
                     /locus_tag="IWO17_001029"
     CDS             complement(11696..12370)
                     /locus_tag="IWO17_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0289923.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(12554..13447)
                     /locus_tag="IWO17_001030"
     CDS             complement(12554..13447)
                     /locus_tag="IWO17_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0289924.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(13521..15218)
                     /locus_tag="IWO17_001031"
     CDS             complement(13521..15218)
                     /locus_tag="IWO17_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0289925.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(15211..16524)
                     /locus_tag="IWO17_001032"
     CDS             complement(15211..16524)
                     /locus_tag="IWO17_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0289926.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(16524..20042)
                     /locus_tag="IWO17_001033"
     CDS             complement(16524..20042)
                     /locus_tag="IWO17_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0289927.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(20335..20793)
                     /locus_tag="IWO17_001034"
     CDS             complement(20335..20793)
                     /locus_tag="IWO17_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0289928.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(21090..21608)
                     /locus_tag="IWO17_001035"
     CDS             complement(21090..21608)
                     /locus_tag="IWO17_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0289929.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(21610..22227)
                     /locus_tag="IWO17_001036"
     CDS             complement(21610..22227)
                     /locus_tag="IWO17_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0289930.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(22224..23078)
                     /locus_tag="IWO17_001037"
     CDS             complement(22224..23078)
                     /locus_tag="IWO17_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0289931.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(23075..23986)
                     /locus_tag="IWO17_001038"
     CDS             complement(23075..23986)
                     /locus_tag="IWO17_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0289932.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(24027..25361)
                     /locus_tag="IWO17_001039"
     CDS             complement(24027..25361)
                     /locus_tag="IWO17_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0289933.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            25569..26399
                     /locus_tag="IWO17_001040"
     CDS             25569..26399
                     /locus_tag="IWO17_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ0289934.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(26643..26892)
                     /locus_tag="IWO17_001041"
                     /pseudo
     CDS             complement(26643..26892)
                     /locus_tag="IWO17_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27083..27277)
                     /locus_tag="IWO17_001042"
     CDS             complement(27083..27277)
                     /locus_tag="IWO17_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289935.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(27782..27967)
                     /locus_tag="IWO17_001043"
     CDS             complement(27782..27967)
                     /locus_tag="IWO17_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289936.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(28817..29158)
                     /locus_tag="IWO17_001044"
     CDS             complement(28817..29158)
                     /locus_tag="IWO17_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0289937.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(29158..29388)
                     /locus_tag="IWO17_001045"
     CDS             complement(29158..29388)
                     /locus_tag="IWO17_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0289938.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            29675..30013
                     /locus_tag="IWO17_001046"
     CDS             29675..30013
                     /locus_tag="IWO17_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289939.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(30218..30457)
                     /locus_tag="IWO17_001047"
     CDS             complement(30218..30457)
                     /locus_tag="IWO17_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ0289940.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(30433..30780)
                     /locus_tag="IWO17_001048"
     CDS             complement(30433..30780)
                     /locus_tag="IWO17_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ0289941.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         9066 a         6589 c         5299 g         9950 t
ORIGIN      
        1 agaggttgtg aaatcagcgt cctgacctga gtattagaga aaaaaacgga aaaatagttc
       61 ctcatatttt gccgtttgtt gagctaatac cgagggtcag ctgattgaac accgtttagt
      121 ggagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaagcagc
      181 aaggagaacc tcatctataa aaagatagaa ggaagtgaga catctattgg tctcatttcc
      241 ttctattttt actttcctca gtcaattgtt ttacatcata aaaggagaaa ttccacttat
      301 ttttttcgct gtttcatgac ataaacaact acaccgatta acaaaattcc tccagctaat
      361 gctagcaacg ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc atttttttca
      421 ctttcttttg ctgtcctatc cggatgagaa ggttttccat ttggttttcc atttggtgat
      481 tcatttcctg gattttctct ttctggtttg tcatttcccg ggggcacatc ggcagtttct
      541 cgcacagtga tcgctttttg ctgggtaaca gattctgtat gagcagacca tttttcccca
      601 tcaaatattt ccgaacgata agtgacttct agcgtatatt ctccttttga aggtgctgaa
      661 aaaatagcgg tatgattaga aaattgagca cttgactgtg tttcaacgat ttggatcttt
      721 tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt gaaagttgac
      781 acaaaaactg taaaaggctt cagttcaatg atatttcctt ctgcattctc cgccacacca
      841 tttgcttctt gtataataat cggattattt tgttcatttg gttttgtctg atttggttct
      901 tgtacgattg catactctgg atattccgaa gacggtgagg tttctccgtc aaaaatagca
      961 cctacagata atggaagacc atccactctt tccttttctg tttgacgatt ccccggttta
     1021 atctgcatca gtccatgaac atggatactt ccattttcat agcgcgtaac aggctgctga
     1081 ccttctttcg aacatgtatt tagagactcg atccaatctt ttgatatggt atgtccaaga
     1141 gtattttctg attgtttctc tcgttcattc caatagtagt tcaacgagtg gtagatttga
     1201 tcttcgtttt gattcaatgg gcggatctgc tctctcatct caagattctc tgttcggaaa
     1261 gataagatac cagaagcatg gtagtctgtg tttcctgctg aagaggtata aagagccaca
     1321 ttatatgaac cgttattaaa acttgtactg ctattgacga taatatctgt tccgctattg
     1381 gaatcgattc ctttcgcaag attttcgaaa gacaaactat tgatcaattc atgagggccc
     1441 gttaaagaag aaccgcccat tttgaatcca ttaccgttac cttctccctc aattcctggt
     1501 acccagccgt ttcgataagc aactgagttt ttgatgatta cttttccgat cgaacctgtt
     1561 tcatttttag caaaaaggtc ccaaccgtca tctgcattat gatacgcgat acaaccgtca
     1621 aatacatttc cttctcctat tgtcagtttg gccgcaaatc catcggcatc ttcaaatcct
     1681 ggatcggcat tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg ccatgcttcg
     1741 aaaggatcat ttgagctacc gctgatttgg attcctgtat ttccattccg ataagcatga
     1801 atctcttcga tcaaattatg atttccagaa atctgtaatc ctttttgcat agctgcacta
     1861 tttgtaatgt caaaccctcg taaaccccag tagtgactga acagtgtcat cccatttcca
     1921 gttcctttaa aatcaagtac aggacgcttg ttttctggat ttttctctgc aatcaactga
     1981 atcgggtttt ctcttgtccc gttaacgcct tttggaatcg tcaatcctct ttcgaaggaa
     2041 taagttccag gcgccatgac gattgtctgc ccagcatatg cgtatctcaa tgcttgtgtc
     2101 agagataatg gattttgtct gcttccgttt cctgcatctg acccttctgg tgttacataa
     2161 actgtcgaat atttaggttt acggtagtcc actgtatgaa gaatctcttt tgtttcataa
     2221 ctagtcatct ctacgtattc ttctggcgga tagtttttat ctggcgtaaa actgaaagta
     2281 aattcgttcg ttccttccat cagttttgtc tgataatcaa attcccgatt cgcagtgata
     2341 gccaagtttt ttgcgttctt aagcacctct cctgttgctt tatttttaat tgtcaataat
     2401 ccgtcagcat ttgctctaaa ggacatagta tgatcttggt ttcctgaatg attactagag
     2461 gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt cttgcccagg aggatctgtc
     2521 gcaggatctg agtaagcaac ttcgattcct tctaccttga tcgtcatatt ccttgctgca
     2581 gcaaatcctg cataaataac cgattcatct gaagcgtata atttttccca gtcatacatg
     2641 atgtcagaag ccatttcttc tcctgtttca ggatcgtaat aatatgcctc taaaccagta
     2701 ttcgtttttt tcatctctac gtccagttta gtaatcgagt tcgtggccgg aaacagattc
     2761 ccatgaccgt ttccgaatat attataagaa cctgtttctt tttccaagaa ccctgctgaa
     2821 gtctctaaag gtgttgtttc cactgagaca ctcccaggag ctggcgcagc ttgcggatct
     2881 tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt atatttgctg
     2941 ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga catattcgat ccgactgcct
     3001 agtatggcaa atgaatttga ataaaatgcc tgcccaaaca aatgattttt tggtactgcg
     3061 tctcttacca ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga ttctatttcg
     3121 atcgtcgccc gcatcctaaa gttgatgtct ttggggattg cttgataata ataggtcatt
     3181 ccatctgatc cgcttgactg gaattttccg ccgttattcg tggaagagag ccgcacgccg
     3241 tcttctaatg gttcaatggc atacctacta gcacttgtgc tttctccgaa aacaacccct
     3301 ttccactctt catttgcttc ttcagattgc ttttttgtct tttcattttc tgattctttc
     3361 atcataggat catttccttc ttccgtatcc atatcttcac tagttcttgg tatttcctcc
     3421 attgttgaca tcccttcaat gttcacgtgt gcattttctt cgttcacaga ctgttgttcg
     3481 ttagcagcaa cttcaacgac agaaattaaa accggggtac tgagcataag gagattcatc
     3541 attagcgcac tgtattttat acgttttttt aacaagtgta ttcctcccgt aacaaattag
     3601 aaagcgttta cttaaagtat tacctaattt atttttactg tcaataaaaa acaaaattat
     3661 aataagtgta ttttataaaa agaaaaaata taacttaagt tgttgataat aaagagattt
     3721 attaacgtaa aaagaataag tttttttaaa ataacgtata aaaaagagca taaaacaaag
     3781 tcgtttgttt tatgctcttc ttttattatt ttgtgctttt tattttatca aatcactttg
     3841 tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc ccattcatgt
     3901 ccaacgggat cgaaacttgc cacggcatct ttgaaaatat ggagtttgta tcctagatta
     3961 tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt caaataaata
     4021 tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa agcggaataa
     4081 tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta taaacttttt
     4141 aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg aaataagcgg
     4201 ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac gacaaaatcc
     4261 ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac ttgtcctgct
     4321 gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc aatcgagatc
     4381 aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc accatttctg
     4441 ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc gagtgatctg
     4501 ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga gctgtttgtc
     4561 caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt aagatttctt
     4621 tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct tcttttgcgt
     4681 tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg cttgcttcat
     4741 aatatagagg cattcctggt aattgtcctg ggttgtagcc aaatcctgtt gcatctgcat
     4801 caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct tcaccgattc
     4861 cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc aacagacttg
     4921 tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact tcgtggttac
     4981 ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt tcgcttaggt
     5041 tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt ttcatctttt
     5101 taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac attaggataa
     5161 caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc cgttcgctga
     5221 tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag ctggcaatct
     5281 ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat gactcgcttg
     5341 cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct ttggccataa
     5401 gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca taaatcgtct
     5461 ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga gttagatgat
     5521 acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc tctacccctt
     5581 gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac atcgaagtaa
     5641 gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta gctgctgtca
     5701 tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc aaaatcactt
     5761 tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg tcaaaagcaa
     5821 tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct ccatctggca
     5881 aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct ttgcgactga
     5941 ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc tttgtaatca
     6001 taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt ttaccataga
     6061 taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac tctttatagt
     6121 agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat actcaacgat
     6181 aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct agcattggtc
     6241 ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact tttattgata
     6301 ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg aaaacaagaa
     6361 gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg tatgacagcg
     6421 aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat caatcaattg
     6481 caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca tgatccagtc
     6541 aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact gcatgaagca
     6601 agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa gtataatgag
     6661 atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga aagcgcgcag
     6721 atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt tgctgaggat
     6781 ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg tgataataaa
     6841 tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg gaacattcag
     6901 atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt aagaatcgag
     6961 aacagattca acaaggagga attttttatg gatcataaac cgactgaaac aaaagatgtt
     7021 acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac tccaacagat
     7081 acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca gacagctgct
     7141 cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct agcagcaaaa
     7201 atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca agcaaaatta
     7261 agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg tcctgtagga
     7321 gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga actgcgtgcc
     7381 ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat ctatgaagtg
     7441 acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa attagataaa
     7501 gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca gaaaaacaaa
     7561 gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat ggaagaactt
     7621 caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga tcagatggat
     7681 gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg tacgcatgat
     7741 cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt gatccaaaac
     7801 acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat tccgctgtgg
     7861 aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt aactgctcaa
     7921 cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat gctgaaaatc
     7981 tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga gacccttcaa
     8041 accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca aaaagaagga
     8101 aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga tctgaaacaa
     8161 aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca aacgcccaac
     8221 aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac ttctccattg
     8281 gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa atgatcagtc
     8341 caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga agcatcaata
     8401 caatacaagt caagatggag acgattggca atagcggaac gaatggcact ttgaattctc
     8461 cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta acaagcatca
     8521 aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg aaaaatccgg
     8581 caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta cgatgctttt
     8641 gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca aacagtaaac
     8701 gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt gcccctacag
     8761 cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc actgggtcat
     8821 tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg atgatgtaaa
     8881 ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt tgcgggtttt
     8941 tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa aacacaagag
     9001 ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag ttatcaggtt
     9061 taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg acatatacca
     9121 tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa gtgacgaata
     9181 tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg tacccgccag
     9241 ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata tagccagacc
     9301 atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac caccctgtca
     9361 gccaggcagc aaattcacca aatacaacat acagataggc atatggccca ccaatcactg
     9421 gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg attgcagcta
     9481 agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc cctgtcacca
     9541 caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca gacgttttta
     9601 gttctttttt catctcactc gtatgattga gattgatctc tttcttacga aacaaatcca
     9661 ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc atatacccta
     9721 aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg agctcttttt
     9781 tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg atccccattt
     9841 gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc atgcagatgt
     9901 ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga tcgactttaa
     9961 ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga ggtaagataa
    10021 ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca taaccaatcg
    10081 tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta tctgtatcta
    10141 tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga agagctaaag
    10201 caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa acggctcccg
    10261 caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca gaaacaccca
    10321 tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag ccggataaaa
    10381 atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag acaactaaag
    10441 aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg ccaccaattt
    10501 taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct attaaaattt
    10561 ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa acgttaacaa
    10621 cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata aaaatttaag
    10681 attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc cgtcaaatgg
    10741 atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt tgaatgtgtt
    10801 ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt caagtcttcc
    10861 gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc ttccttttct
    10921 atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact aagcaattgg
    10981 ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc accaacgatc
    11041 tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac aggctgaatg
    11101 attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc gagtcttgca
    11161 taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc tagtaatgta
    11221 tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga agcggaaagt
    11281 ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg ttgattactg
    11341 attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata ttcttctacc
    11401 agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga agttttttgt
    11461 atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag cagataaaaa
    11521 atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc aactttgatg
    11581 tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca tcccaagatc
    11641 aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca tcctttcagg
    11701 gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct gcctgttcga
    11761 tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca aatcgttcgt
    11821 catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg tgcttcatca
    11881 aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg ttgatttcca
    11941 tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca ctagataatt
    12001 ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt acatcgattt
    12061 ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc atatccatat
    12121 tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt attttttgac
    12181 accaataata gccatccaaa acgggaagat taatatctag taatacaagc tgtggttctt
    12241 ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt tcaaattgcc
    12301 atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg actaccatga
    12361 tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca aagaagccct
    12421 tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa aaaaactgga
    12481 aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta tgttattctt
    12541 aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta aattttcgat
    12601 aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt ataagtaatc
    12661 gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct gtaaagggaa
    12721 ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta caagtgataa
    12781 gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt ggaaggacaa
    12841 caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga tagaatcggt
    12901 catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata ccgctatggg
    12961 cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct tgcaggactg
    13021 tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag ctaacattga
    13081 ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt cctggatttt
    13141 ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct gggattactg
    13201 atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc tcatgttttt
    13261 cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga tccaaataat
    13321 tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca agaaacatca
    13381 ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta cctattttct
    13441 ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata aaaccgattg
    13501 ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg gtaccagctg
    13561 tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc gttcccacca
    13621 gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg attttctact
    13681 cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc agtaaaggct
    13741 gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat ttgataggtt
    13801 ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa ttctgttgct
    13861 tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc taaaggcgac
    13921 tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc gaatcttgca
    13981 tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc acctgttgtg
    14041 actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg tccgatttca
    14101 ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac cttcaaatta
    14161 tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt atctactttg
    14221 gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac tacttctact
    14281 atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat ttcctgatca
    14341 taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat aacagcgctt
    14401 ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt gtaagaagcc
    14461 gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc ttcattaata
    14521 atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc ttctctgatt
    14581 tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccatgt agaaccatcc
    14641 cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat tggagacgcg
    14701 attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt atctctagaa
    14761 gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc ttcattcgtt
    14821 gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt gtgccgtttt
    14881 tgaatccatt caatcgcttc ggcagaatta agaccgtctt ctcgttccgg tgttaaagga
    14941 ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa atcgactcct
    15001 ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg ttcttccgga
    15061 acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt ttgtgcgact
    15121 tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt aacgagtaac
    15181 agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc gttctttttt
    15241 tattaataaa acagcaccta acaataaaag aattcctagt aaagaaatca acgttcttgc
    15301 ttcattcgtt tgcgggaata cggaccattt tcctgattga cctggattgc ttggaccact
    15361 cggctctttc ggtttgtccg ttgtttcttt tacggtctcg ttataaacac gtggtgttgc
    15421 ggattcatag gcagagactg gaactttccc ggattctaat tcagccatct cctgtccgtt
    15481 aactactaca tattgtggtt ggccttttgc atccacccaa aattcaggga tgatgacctc
    15541 gattctctta cttgcttcat ccatctcgta accttccaca ccttttaatt cttcaaaata
    15601 ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt tatccgaaat
    15661 gaatttgctt acatttttgt cattcaaagg atcattgtct gctggtttga tccattgatt
    15721 tttcaagtca ttagcaggag ccatatctaa ataatatttc cccccttcta tcatctgata
    15781 tagagcaaaa acagcacctt ctaatggttt tcctttttca gttgttcctc tttgacgtcc
    15841 atacttgaag aagtatggat ctcttagata accgacattt ttaggataga tatgaatctc
    15901 ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc aaaatagcag caattgggat
    15961 agctgtctga tcgatatcaa catccaatcc aacttctgga tcaatcgatt cttcgaaaat
    16021 caaataaact gcatcccgat cttggttttt cttaggtgct ttgatacgtg caatcccgtc
    16081 tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa gtccctattt ggacaagcct
    16141 ttcttctgtg attagctgac ggatattgtt tctgttcgtt tcagcgattt gctggacgaa
    16201 ttctttagga gtcatgtttt cagcagcttt tttttcatga taataatcgg tcatgtcgta
    16261 aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa atgatatctg ctgtttcccc
    16321 tgcttcctca gagatagcca agccatcatt ttcatggtat tcaaattgat cgttataatc
    16381 gatatctcga atcatccgtt tatgcaacac gaattccacg acatcttccc ctgattcttc
    16441 tgccgacatc gcatgtggaa gaaggactgc tgcaagcggt agtaaaacaa gtaattgaag
    16501 catcaaacac aacagctttt tcattacctt gctccctcct ttttagattg tctgacaaac
    16561 aaaccatagg ctgctcctgc aagcgccatg aagaatgcag caagcaggat atagaataat
    16621 cgtccggatc ctcctgtagc aggcaacgga ttttcaggat cgttcccgac actaagctgt
    16681 aatctgattc gatttgtctc caaaagttca tattctaact cttgctgtag atcaggcgtc
    16741 tctgcttctt cgtttggata atacgaaatc tcaactttta gtgtacccgc ttcttttcct
    16801 ggaactaccc gatcattttc tatgtcaggt tcttctcgat aactaatttc taaaataaag
    16861 taacctggaa gttttctata gctgtcaggt gacttcactt ccttgatccg atagacccct
    16921 tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg gatctggtgc caagcctccc
    16981 tcgatgattt catactctcc tgcactattt tgtttctcaa ttgaaaattc tgcgccttca
    17041 agacgtcggc tattcatact gtcaattttc aaaagttcca aatcgattgg cgctagttca
    17101 tcttctaagg ttacttgtag tacaccatcg atgatttcat agccttccgg caagggagca
    17161 gtttcgtctt cgatgaccgt atcttcaatg ataaacttac cttcttcatt cagtgcaaac
    17221 tggaacactt gatcagatag ccgataccca tttggtgcga cagattccga gacctcatat
    17281 tcaccaatag acagatgcag aggttggtcg taatttaaat cgacatactt ttctactcct
    17341 acggcacgag aatagaagta tccgatccca ggcaaacgtt tcgattcttc tggttctaac
    17401 ggattaccag tgtatgctcg atacagcggg aaggaagaat cccccacttt cctgatatcg
    17461 aaacctgcac catctaatgt atggccttca tggtctaatt ttctaactcg gatctcccga
    17521 taatcaaacg tattgtaagc agtgaatgct acccataaac gatcctcttc atctgttgta
    17581 tacgtgagat attgccaatt ttcttcacct gtttgcttga gttggatagt ccctatagca
    17641 ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag gttctttgaa accttctggg
    17701 ttctggctct caatgatttt gtattctgca tagttggtag tttcttcact atccttataa
    17761 acaaatgaat ctggctctcc gattggatga ataactttct caccatccac gattacatat
    17821 ttcccgtctt catctactga atagaacgtg agtctccctt tctcttcctc tacttcgttc
    17881 acaccagaaa gccctttcgc tactggatat ccgatttcca gtccgttacc atctgtttca
    17941 taaagttcaa actctgcgcc attgattggc atattactgc cttcaattag tttattgata
    18001 ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt tgacatacaa ctcatcatac
    18061 tctgtgcctt ctatgttagg ctcttgtaca acagaaagct tgtgatattc tccttcataa
    18121 taggaattcg gatctttacc ttccggtccg ccgactctcg aaccagatgt atattcatct
    18181 acaagctcgt agtgacctaa catctcatct tcgttggctg catatttttt gatttggaat
    18241 tgatacgtct catctactaa ctgataacgt ggtggtccag cgatctctct gatccaaaat
    18301 atgtcgctga ctgctccagt ttcatctcct aaaatcggca tcttcgtttc aaagatcgct
    18361 attccgtccg tggtagtttt acttgtttcg attggttcgg cttccccact ctctggattt
    18421 tggtcatata atgcaaattc gactccagcc aacggctcgc cattttgacc cttcttgctg
    18481 acttggaaat tacggccttt atgttcgtaa cgaatggaag aagatgggaa atacatccgt
    18541 ccagtagtga tatcattctc ataatcctcc aggttctgaa tataggccat attgttcact
    18601 gtgcaaaaat ttccgtcttg agctcccgca tcaagtttta cttggtactt gatcctgacc
    18661 atttcccctt cacctaaaga aatcggagaa aatttataag cttggttttc ccgtccgaca
    18721 tacgttgtcg gagtcgccac tacttctggt ggggcttgtc catgacgttc ttcatcccat
    18781 atttctggct cttggtactt catgccgttt tctcctggat ctagatggta aaactgaata
    18841 tctgccgatc tgccagctcc aaatggtgag acattaaaac cagtttcatc ataaagtgtg
    18901 gcttgctgga tcgttctctt gaaggtagaa gagatatcct ccaaggctgt aacaatgtca
    18961 ttcttgttgt tcgcattgta atatcgcggt gttacgccat cttcttctaa atcagcaata
    19021 tttttcaata catttcgtcc aatagcatct agtcgttgtc tcccgctaac gtttccatcg
    19081 attcctaaac caatggagaa gatacttggc gggttctctt gatacttgtt tttcagccaa
    19141 tgatgataag caactgtgtt gatcgcagat gacttttctt gcgaagtagg actgccattc
    19201 gtattgttat tagaagaacc atcaccaacc cagtgagtcg ctttattatc tccttctttt
    19261 acctctccat aacgatagtg tacatttact tcatcttctg gacggttaaa gtcagatgca
    19321 taggtaactg gatgagtaaa ccctgcatta gaagtattcc cagaaaactc ctcaaaattt
    19381 ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca tcacagtcca gttgctccaa
    19441 gctccattag tgctcgtacg ataacttgac tgaatcggtg cataactgaa ggtcggtgtc
    19501 ccatctccca caacgattag gattttttct ggatttctat tttctccgcc attgtcttta
    19561 tacaatgttt catagccatt tttaagccct agtgtaagcg gagttcctcc gattggtgta
    19621 cgatttaatg ctgtattctc acttcccgtc aaacgattga tatcatttgt caatggaaag
    19681 atgtccgaac tgatttgctc atggttattg atagctgtac tattatggta aaaattcacc
    19741 ataccgattc gaatatccag actcgggtca gagagcagat ctttactcat ttcgtttact
    19801 gcctcaatca acgcagcatt tttagtttga ctattggtcc cagcggttaa ttcactcata
    19861 ctcgctgact tatctagaac aaaaacgata tcgattggac tgatttcctt tttctcgccc
    19921 ccgatgacat ctagatagac attgtaaagg gttgttgcat cttcaggatg tagatcagat
    19981 gctgtgctgc ttggcagaac aggttctacc cattttttga tgtacgcccc gttataatcc
    20041 aagtaactgt tttcaaaatt ccgttcattt ccccaaagct gaacggtttg agctggaacg
    20101 tttctatctt cttcgcctgc agttgatcca ttattagtag catagtcgta atttcgccag
    20161 ttcgctcccc cgtttgtata atcactatca tcgatcaagt tctctccgtc atatggcgag
    20221 cctttgtctc cttgatagcg ttcattgctt tgtgtccaat ttcctaacac ttttgtatag
    20281 tcattcgttg taaacttcat ctattctttc atcaaccacc acttgaagag gtagttaccc
    20341 ttcgttcaca tcggggatct tttctgtttc aaaaatcaat gtgccggttt ctgcttcttt
    20401 tgaataaact tttccgacat accattcttg accatcgatc gtctcgactt ttaggtcttc
    20461 tccatcttca tcataggagg atagacctgt tccacgaacg ttttttacgg taccaatccc
    20521 ggcggctgcc gcatcgattg ctaaacgtat tctaccttca ttggcacttt cctttttttc
    20581 aaaagccaga tgccattcaa gggtttcttc tttttcaata tacttccctt caatttttgc
    20641 atagtcctca tctaagaata cctcatcctc ctttgtttct gttgactcag cagctacatc
    20701 cagggtcgtc atatgataga ctgactggaa atatggtcca aatagcaaac tgacagttac
    20761 taactgcaaa agcttcaatt ttaacccttt cacatcctta tactcctttc aaaaatattt
    20821 gtcttctttc acaacaatca acaaaacaat gcccatctaa ttataattta atttaatttt
    20881 aattttaaat caaatgatat cctttttagt taaaaacacc aattatacaa catttattat
    20941 taaaattaaa ttataaaatt ttataacgtt ctcatttaac aaaacttaag ttattaagac
    21001 aaatgaataa acaactacgc tttcctcgaa acaaaaaaat aaagcacaaa tgcgcattta
    21061 cgcactcatg ctttattcgt acaattaatt cagataacgg cagcgcatag gatgccaata
    21121 acacttccta aaatagctcc tcctaaaatg tcagtaggat aatgtaaaag taaaaaaatc
    21181 ctcgaataag cgatcaaaca agcaaataaa aaggctaata ttcctgcaac tggacttaat
    21241 tcgaacaacg cagctactga taaaaaggct agcgctgtat gtcctgaagg aaaagaagaa
    21301 gtcttcggtt tacttgtgta aaggacaagc cgacgatttt tttcataagg tcttttccgt
    21361 cttgtcgttt ttttgataaa aagcaaacca acaaaaaaat agatcaaact gccgataaga
    21421 atggctagac catattctct agtatcaggt atcaagacta aaacagcagc aaggagcaac
    21481 caaataaaac caccattaga gatattactg ataaatttca aaaaattgat ttttcgacta
    21541 tccgttttcc gttgcttggc gaacttgttg ataatactat catcgatttt agcaatcaaa
    21601 cgcaacatgt tattcctcct ttttaagaac cttttcttgc gtacgatgga taaaaagcag
    21661 gacttgaaaa taaaacaaga aactgaaacc taataaagca aaatagattg gtcctgcaaa
    21721 ccagaatgcg acaagaagga tcaacgtgcc tgccaaatag aaaagcgcgc ttttgaaatc
    21781 attaaaaaac aagaaaaatg cagtaatcca agaagcttct ttttgttcca gttgaagcaa
    21841 gattttgtaa ctaaagagaa gcgcgcttaa tagaagcaaa aacaacgtag tatacatcaa
    21901 tgtatagcca attcccccac cccaatagcg gaaaatctga taatcgatca atagaagaag
    21961 aaaataaaca cttgctaaac tatcttgctt tccataaaga gagaagttct cacgaaactt
    22021 tttccagaat agtgaaccga gctttttctc ttcatttttt ttgtaatgat aaatcatata
    22081 cagaactgtt cgacttgcag ttcctaatgt gaaaacaggc aagacgcaca ggaaccatag
    22141 tagctgtaaa tggataagac gtggaatcca ttcaatgatt tctaagaaat aatcattggc
    22201 ttttttacca atcgatcgat tcatcatcct tttgaacctc ctgtcaaatc catcccttca
    22261 atgaaataac gttgtgcaaa aaagaacagc aaaatcgtcg ggatcaagac aagtgttgct
    22321 cctgccatca gataattcca ttgtgtcgtt gcttggttct gaaaaacttg caagccaatc
    22381 tgtaacgtgt atttttcttg atcttggata tataacagag gacctaagaa atcattccaa
    22441 gctgcgttga aggaattgat cgcaatggta atcaaggcag gtttcgtcaa tggaagcatc
    22501 aaatgactcc aaatatacaa atgattcgct ccgtcgattt cagcagcttc gatcaattca
    22561 ttattgatcg ataagtaaaa ttgacgcatc agaaatatat tgaacgcatt gccgaagaac
    22621 gatggaacga tcaggggcaa ataagtacct acccagccga tcttggagaa taatacatat
    22681 tgagggatca tcgttacaaa ccccgggatc atcatcgtag aaagaaccaa agcaaacatc
    22741 agctttttcc caggaaaatc aagcttcgca aatccataag caataaaaga atttgaaagg
    22801 acattaccga tcactactag cacagtgatg aataatgtat ttcttgcata gcgccaaaaa
    22861 ggaaaggcgg caatcgtatc cagatagttt tcccatacga ttttttctgg gaaaaaggtc
    22921 ggaggataac gaacgatttc ttccattggt ttcaatgaag taaacaccat ccaaagcaat
    22981 ggaatgatca aaatgattcc tattactgtc atgaggacat aacttagtat tttttttgtt
    23041 tgccgattca cactggtctt tttgaaataa tccatcattt gccgtcacct ccagaataat
    23101 agacccactt aggtgcaagc ttcaaattga caaacgtcag aaccaacacg atcagaaaca
    23161 ggatcattga catagccatc gcataaccca tatcaaaagc tacgaacgcc ttgttccaca
    23221 tatgcagatt ataaaaaagc aaagaatttg caggtccgcc tgttccattt ttcgtcatga
    23281 cataagcttc ttgaaaaatt tggaaagaac cgatcgtaga ggtgatcaca tcgtaaaaaa
    23341 taataggagt gattaatggc aaagtaatac ttttgaactt tccccaaacg cctgctccat
    23401 cgatttctgc tgattcatat aaagatctag gaacattttg taatgtagct aagtaaagta
    23461 gcattgctcc tccagaactc catattttca tcaaaatcaa cgcaggtttc gtccattgtg
    23521 gatcaaacag ccaagctggt ccagggatat ggaaccagcc cagaaatgtg ttgatcaatc
    23581 ctgcagatgg agaaagcagc tgcatccata agatgtagac tgctactcct gataagactg
    23641 ccggtaaata gaaagctgta cggaatacac tgattccttt gattttttga ttgagtactg
    23701 tggctaaaaa cacactgaaa atcgtcgtca gcggtacatt gaagatgacg aaataaacgg
    23761 tgttgtataa cgatttccaa aataaatcat cgtgaacgaa cattcttttg aaatttgcta
    23821 gaccaatgaa attcatcttg gatgtcaaat tataatcggt gaaactacca ataactgaaa
    23881 aaagcatagg ccctagcata aataccaaga acccgataat aaaaggcgag ataaataaat
    23941 aggcccatct tgcttctttt ttctttcgga tagataaacg cttcaaattt tcttgcctcc
    24001 tctgtaccta gacagtcaaa aatggattat tgattttgct tgactaagtt ttctactgct
    24061 ttttgtgcat ctgccaatcc ttcttttggt gattttgttc ctaaaagaat ctggtcaatc
    24121 tgttcattca ctaatgtcgt gtaatccgga gcatctaaag gaactggtgt gatcaccgta
    24181 tttttaaagt tttcgtccgc catttgatag atcatttttc ctttatccgt taggttgtca
    24241 ttattcacta aattctcatt ggctgtcttg ctcgccatga tatcaaaact tttttcgccg
    24301 aatttttctt gtacttctgg agaagaaaga tatttgatga attcataaga ggcctcagga
    24361 tcttttgctc cgtatggtac ttccaagacg aatccgccac cccacgacca atggcctgaa
    24421 ccttcttctt tttctgggag ctgcatcacg ccaaaatcaa aatcatccgg tgcattttcc
    24481 atcaaactag agtaataatt gatattttgg gcacgcatgg ctactaatcc ggaaataaat
    24541 ggatcagata caccagaccc aaattcggct tctaatttat tgatcgtatt tttcccataa
    24601 taatcttgcc agtcttggat ccactccaag gcttccactt tatttttagt atcaatcttg
    24661 actttttcat ctttgtcaaa ccaactagtc ccgttatctg cgtttagcgc ccatacatca
    24721 gcccctaaat tccataatgg ataaaaaccg atgcgtgtaa attcttttcc ttctttttta
    24781 tctagtttac gtgctgtttc ttctaattca tcccaagttg tcggtagatc ttgttcactg
    24841 attccagctt ctttaaataa ctttttatta taaaaaatga cttgtgtatc tgtattgaat
    24901 gggatcgcat aaggatcacc ttcatattct actgtatccc aaagctgcgg ataaaaatcc
    24961 ttagaagtgt ctttttctac atatttggag agattagttg cttgctgtgc ttctgctctt
    25021 tgggcaacgg aatttatgtc ctgtacaata acatcgggtg catttccagc tgttattgca
    25081 gataaagatt tagtccagat gtcaccccat ggctgatact tataatttac atggatctcg
    25141 ttttgtgatt gattgaaatc ctcaatgatt tcttcgatag cttcttttcg tgcaccggat
    25201 ccccaaaagg accagaaatc aatatttttt cttccatcac ttgttttttc agaagaattt
    25261 gctgcacttg aactggcatc gcttgcatta cttgatccgc atcctcctaa taaaaacaat
    25321 gctgaacttg ccagcaaccc tgtaaacaat aattttttca tattttacct ccaaatttta
    25381 atgtaaccga tgccggtttt aatataacga tatcattttg ttctgaaaat aacaatttat
    25441 aaggtatgat tctaaaaaaa ttattaatga taccaatact ttatataatt cgcgagcgct
    25501 ttctttgaat tttttaatct ttcgtagcag aatgagaatg tattaaaaat agattaggag
    25561 gaattttgat gtttagacac cagaaagaat tgcagtttga agcaaaacca gatcgtccga
    25621 atccattaat cgcgaaatat ctgcaggagc tgatcggcgg ccaatatggc gagatgagtg
    25681 tagctatgca gtacctgttc caaggctgga gctgccgagg agaagaaaaa tataaagata
    25741 tgctgatgga cattgcaaca gaagaaatcg gccacgtgga aatgttggcg acaatggttg
    25801 ctcgtttatt agagggtgcg cctgttgaag atcaagaagc agcgatgcaa aaagatccaa
    25861 caatcggtgc cattatctct ggaatgaatc cacagcacgc gatcgtagct ggcttaggac
    25921 ctcgtcctgc tgacagcgtg ggcaatccat ggagcggtgc ctatatcatt gcgagtggca
    25981 acttgttagc agactttaga gctaatttaa atgccgaatc acaaggaaga cttcaagtag
    26041 cacgtattta cgaaatgatc gatgatcgtg gcgtaaaaga tatgctgtct gttctccttg
    26101 caagagatag ctatcatcaa aatctatggg aatgtgcagt gaaagaatta gaagaaaaag
    26161 aaggttcaat cttagtccca agtactttcc cgcgtgacaa agaacgaaaa gacattgctt
    26221 atgacttcta taacttctca gagggagacc aaagtagtga aggttcttgg gctagtggca
    26281 aagcactgga tggcgaaggc gaatatcaat ggttgaaaga acctaagatc gaaggcgctg
    26341 caccaaaatt agaaccagta acaccaaaaa tgtatgggac acctccattc aagaaataaa
    26401 tatctacccg tagaaacacc cgttttttgt tccacacgct ttgtgagcaa aactgactaa
    26461 agtcattaat cataaccacc cttcaaaagg gtggttttct ctagagctat aagctcaaat
    26521 caccggccag cgcctaaaga cgctggcttt catattttat gttcaagcca tattgccttt
    26581 gccagttttg cccacctctt aaaaggttta tgtactactc cttagccctt aaaaggatcg
    26641 tcttactctc gtacactcag cttatctaac gcactatcat gtttttcttg ttcttgaatg
    26701 tactttttga tggtcgcttc attttgtcct acttttgtca catagtatcc ttccacccaa
    26761 aagtgtcgat acccaaattt atattttaga tttgcacaat ctcttgatga tctcctgaat
    26821 attggctcga tattgattat aaattatttt tcttctatac tttggtgtga atacaatgtg
    26881 gtatttacac atctactttg tacgggctaa actatttccc tttattgctc cacccgcata
    26941 gcaagtggtt ttgtatttca atcgctcggc gcgctcaatt aggtctaaag acaataaatc
    27001 taaaaacccg ctgcttcact gacagaaaca acgggttttc agaaaaaaat gaaatgaaaa
    27061 agatctctta cggcaatcta ttttattttt ttgatttttt ataaatggaa aatgcttcaa
    27121 gaagtccaag aaataaagcc gtataaaaac aagaacctat gaaatcttta tttattagat
    27181 attcaacaga aatcccgata acactagcca acaatatatt tatcagaagt gtaaaataag
    27241 gatgcttttt atagaataga actagtgttg ttttcataga ctctcctttc ttcatatttc
    27301 caattaatca caaattcacg gcattactag aaaaattaaa ggggcgcaga agtggaaaaa
    27361 tactcttcga ctttccctac aaaccaggcc atcttgtgtt taataactat accaaatcta
    27421 aggtacctct aaatctaaat tccatttgtg gcggtgacct ccaattttta actcttctat
    27481 ctcccatcca gtatacataa ccttgatact tcgaccactt atatatcgaa cacacaaata
    27541 cctatccatt gcaggagtac tacctaactt agttgctact tgcatctaaa taacagctga
    27601 aaagatagga cttactctta gtaaagtatc tgaactatta atttttaaac tatcattttt
    27661 atcctcaact acatgcacat ctgttgcaac ttcactttct gtctcatcca gataaatgat
    27721 taaggaatcg ggaattgcaa tatcttcagc tgacgctgga atagttgagc taaattagtc
    27781 atcatatctt tattataaaa taaccaacag caaaaaatac aatgaaaaac aaacttggaa
    27841 ctaatgtatc attaataaat gttgaaaaat tcagcaactc attactgtat gattttagca
    27901 gcccaaccaa catataccct ataaaagcta ataagatggt taggaatcct ttagaatttt
    27961 tactcattca tagacacttc cttaaataac ttttattata gaaggtattg taaataataa
    28021 tattgacaaa aagtgactca aaataaaaaa aaacttacat tatactggaa tgaactgtat
    28081 tgatagtgta cctaaaagct ctactgacat aagatcgtgt aatcggtaaa gtaattttat
    28141 taattatacg ggatgtaatc aattagagaa taaactaatc ataaaaggta cttttacatt
    28201 cataattgtc gctttgagat tctactttgg ttcactatct gacccaattg cacattttcg
    28261 atcattacaa gtattggtgt cgttagaaca actaaataaa aactagcggt agatttttca
    28321 gaatctatgc taatttctgt atggatgttc cagtgatggc aaatttctct tttcattgtt
    28381 tttcttgtcg catgatatgc ctttttgtgt tattcagtta aactatagaa attctttcct
    28441 agacagatct tgatcccaaa ttttacgttt ttgagcatcg atatagttta aaatgacatt
    28501 actcatatct aaagagttta gattttttag gactaaaact gtcagttgtt ctttttcttc
    28561 tttctgttaa agtattgtcg cgacttttcg tatacgttaa gatatgtcgt gaaaaaacaa
    28621 gttttgtgac acaaaaaaac gaatgacttg aggaaatcac ctcaatcatt cgtttaaaat
    28681 gctatatatt ctttagttta agatacggaa attaatgcca tcgaccacac tagtagaatg
    28741 aacgacattt acttggcctt tttttaattt cgttatgttt agcatataaa aatattatag
    28801 agtttactta caatgcttaa aacatatact gtataatctg atcaatttta gccatgtctt
    28861 gcaatggtaa gttttctatt tttttgagtt ttcgttcctt aaaatcaaga gattttaatt
    28921 gtgtaataat tacttgtcct tttatatcta gctcttcagg taatgagtaa cgtgtcggta
    28981 aattctttat agtggaagta attggacata tgaccgaaaa cattgtttta cggttaaatt
    29041 cataacgaga gacgactagt cctggtcgtc ttttttgaat ttcttttcct acggagggat
    29101 caaaatcaat ccatacaata tcaccttttt taggaatata aattctttct tctgacacta
    29161 taaaatttct ctcccttcag gagttaaatc ttcccattcg tctttctcat aatactcggc
    29221 ctcttcccca ccactaaatg gatcttcaat ttttggaact aacgtaattg ttccatcatc
    29281 agaatacaca acgatatatt cttggttttc tgaaggtttc tttccgttat cagaaggtaa
    29341 agttaccact acagaacttc cttgtaatct agattttgta acaagcatta tcatcactcc
    29401 cttaataaaa gtatactata taacactaag taacgcaaaa ttcccctttg taaactatct
    29461 agaaaattgt tttgtttaaa gtatatctta ttggatataa atagaaataa attgtagcca
    29521 gttcattttt tctttaacta aaaaaataat ccaagaaaca ttgaacctat ggatccaaaa
    29581 gctacccaaa tactatagct aaccccccgc taccttcaat tttgcagata aaacgctttg
    29641 ggagaaaatt tcaaagacaa caggccatcc tatcatgccc gattataaac aaaaagctgg
    29701 ctattccttt tgctctgaca aatttcaatt gggaaagttc acgaaactag caggtgaaga
    29761 agtttcaact gttcggatca aagaatgccc gcttcaaata gaaacattgg ttcaacgaat
    29821 ttcaactaga caagactttg ctattgtaga acgtgagatc gtagctattt ttgtagaaga
    29881 agggatttta catgatccaa ctcatatcaa tgtggataaa tggcaacctc ttatctataa
    29941 attcagagaa tatacaactg ccaaccagtc gttaactttg aattttagat tccaagaatt
    30001 caaagagtca taatgataaa catcgttagg tataaataaa aaagactcgc agcacgagta
    30061 aaaaacaaat agaagctata cattagaaag gtcgactgtg accctataga tgcagtatat
    30121 gcagcttatg agcatttctt ctgttctctt tgtttttggg atctgtgctg aagtcttttt
    30181 cgttttttgc cgctctttct ttgaaaaatc gattatgtta taccttctcc attactaatt
    30241 caggcatctg ctgagtaatg atttttcgcg caaaagaaat atctgttacc caaggttcga
    30301 tttgttcttc agtcaagaca agcggcattc ggtcatggat tggtgcgact gtgcggttag
    30361 gttcagtagt cattatgatt gattctgcca ttttttcatc ctgatggcta cgataaaaac
    30421 cgccaatata cactattttt tccttatttg aaaacataaa tttgtctttt tcttcattcc
    30481 actcattggg aaaacacaac gattttgcaa aaatggttga tgaaaggtct ttttttcttc
    30541 tactgtctct gcacgggcat taatcatcaa ttgtcctttt ttaaatccgg taaatcccca
    30601 cttcatgatt tccggagtga ggatgttttc ttcctctttt cttaacgtca caacaagatt
    30661 tgacggaaaa acttctccag ctgctaattc tgctgcctgc ttcggttgtt tcgtctgggc
    30721 ttctcgataa taattctgaa ggagctctga ctctagatca aaaaaataac gaccacacat
    30781 cctaatccct cccaatcttt ttttctatca tacactgcaa caagaagttc gcaccaacaa
    30841 aacgctttca atttttgtag aactaaaaaa gttgtgacaa gtgacaaact ctccagtgaa
    30901 tggt
//
LOCUS       DACECR010000025        29970 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.025, whole genome shotgun sequence.
ACCESSION   DACECR010000025 DACECR010000000
VERSION     DACECR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.025"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IWO17_001049"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IWO17_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ0289942.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="IWO17_001050"
     CDS             complement(2944..3378)
                     /locus_tag="IWO17_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289943.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..4600)
                     /locus_tag="IWO17_001051"
     CDS             complement(3395..4600)
                     /locus_tag="IWO17_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ0289944.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5294..5680
                     /locus_tag="IWO17_001052"
     CDS             5294..5680
                     /locus_tag="IWO17_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0289945.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5774..5965
                     /locus_tag="IWO17_001053"
     CDS             5774..5965
                     /locus_tag="IWO17_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0289946.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6479..6811
                     /locus_tag="IWO17_001054"
     CDS             6479..6811
                     /locus_tag="IWO17_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0289947.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7107..7262
                     /locus_tag="IWO17_001055"
     CDS             7107..7262
                     /locus_tag="IWO17_001055"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0289948.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7538..8878
                     /locus_tag="IWO17_001056"
                     /pseudo
     CDS             7538..8878
                     /locus_tag="IWO17_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9119..10735)
                     /locus_tag="IWO17_001057"
     CDS             complement(9119..10735)
                     /locus_tag="IWO17_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0289949.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10853..11071
                     /locus_tag="IWO17_001058"
     CDS             10853..11071
                     /locus_tag="IWO17_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0289950.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11266..11727
                     /locus_tag="IWO17_001059"
     CDS             11266..11727
                     /locus_tag="IWO17_001059"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0289951.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11920..12159)
                     /locus_tag="IWO17_001060"
     CDS             complement(11920..12159)
                     /locus_tag="IWO17_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289952.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12183..13706)
                     /locus_tag="IWO17_001061"
     CDS             complement(12183..13706)
                     /locus_tag="IWO17_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0289953.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13724..13846)
                     /locus_tag="IWO17_001062"
     CDS             complement(13724..13846)
                     /locus_tag="IWO17_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0289954.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13876..14859)
                     /locus_tag="IWO17_001063"
     CDS             complement(13876..14859)
                     /locus_tag="IWO17_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0289955.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14883..15032)
                     /gene="rpmG"
                     /locus_tag="IWO17_001064"
     CDS             complement(14883..15032)
                     /gene="rpmG"
                     /locus_tag="IWO17_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0289956.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15053..15430)
                     /locus_tag="IWO17_001065"
     CDS             complement(15053..15430)
                     /locus_tag="IWO17_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289957.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15462..15641)
                     /gene="rpmF"
                     /locus_tag="IWO17_001066"
     CDS             complement(15462..15641)
                     /gene="rpmF"
                     /locus_tag="IWO17_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0289958.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15656..15925)
                     /gene="rpsN"
                     /locus_tag="IWO17_001067"
     CDS             complement(15656..15925)
                     /gene="rpsN"
                     /locus_tag="IWO17_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0289959.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16448..18220)
                     /locus_tag="IWO17_001068"
     CDS             complement(16448..18220)
                     /locus_tag="IWO17_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0289960.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18174..19928)
                     /locus_tag="IWO17_001069"
     CDS             complement(18174..19928)
                     /locus_tag="IWO17_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0289961.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(20038..20607)
                     /locus_tag="IWO17_001070"
     CDS             complement(20038..20607)
                     /locus_tag="IWO17_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ0289962.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20625..22049)
                     /locus_tag="IWO17_001071"
     CDS             complement(20625..22049)
                     /locus_tag="IWO17_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0289963.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(22051..22719)
                     /locus_tag="IWO17_001072"
     CDS             complement(22051..22719)
                     /locus_tag="IWO17_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0289964.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22849..23433)
                     /locus_tag="IWO17_001073"
     CDS             complement(22849..23433)
                     /locus_tag="IWO17_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0289965.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23764..23997)
                     /locus_tag="IWO17_001074"
     CDS             complement(23764..23997)
                     /locus_tag="IWO17_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ0289966.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(24012..24962)
                     /locus_tag="IWO17_001075"
     CDS             complement(24012..24962)
                     /locus_tag="IWO17_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0289967.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(24959..25801)
                     /locus_tag="IWO17_001076"
     CDS             complement(24959..25801)
                     /locus_tag="IWO17_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0289968.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(25803..26522)
                     /locus_tag="IWO17_001077"
     CDS             complement(25803..26522)
                     /locus_tag="IWO17_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0289969.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            27214..27372
                     /locus_tag="IWO17_001078"
     CDS             27214..27372
                     /locus_tag="IWO17_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0289970.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            27444..28670
                     /locus_tag="IWO17_001079"
     CDS             27444..28670
                     /locus_tag="IWO17_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0289971.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(28758..29150)
                     /gene="rpsI"
                     /locus_tag="IWO17_001080"
     CDS             complement(28758..29150)
                     /gene="rpsI"
                     /locus_tag="IWO17_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0289972.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(29166..29609)
                     /gene="rplM"
                     /locus_tag="IWO17_001081"
     CDS             complement(29166..29609)
                     /gene="rplM"
                     /locus_tag="IWO17_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ0289973.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT         9809 a         5533 c         4360 g        10268 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4501 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4561 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4621 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4681 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4741 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4801 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4861 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4921 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     4981 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5041 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5101 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5161 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5221 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5281 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5341 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5401 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5461 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5521 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5581 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5641 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5701 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5761 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5821 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5881 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     5941 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6001 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6061 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6121 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6181 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6241 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6301 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6361 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6421 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6481 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6541 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6601 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6661 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6721 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6781 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6841 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6901 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     6961 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7021 caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag attgtcgtcc
     7081 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     7141 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     7201 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     7261 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     7321 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     7381 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     7441 ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa tcctttagaa
     7501 taaagtcgac gaaaaccatt caaaggagtg tttcaagatg tcctattcag agtttaccaa
     7561 agagttacta gatattctag acctaaatct tatctttcat gaagatgcct ttcgcaaaga
     7621 gcgaatcaat gacgaaacat gttttgtttt tgatggcaca ttgacctatc aaccggagga
     7681 atgctttcat cgccactatc aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt
     7741 atccatctta ttgaatgatg tcagtaacta caagaccatt ctgcggatta acaaacagcg
     7801 cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta gtgatcgtcg
     7861 ctgctccatt gctcgcagag tcaaacaagc cattcttgag ctattaagcg aacctctttc
     7921 tatgtctttg atcgcacgta tgaagcacat ttctccgacc actgtcattc gaatacttcg
     7981 cagccttcgg cctaaaacca tttctttgag ccagctgtta ccagaagtgg tgtgtttcga
     8041 tgaattcaaa tcagtgaaaa atgtctcggg agccatgagc tttgtcatga tggacggcaa
     8101 gacccatcaa ctgattgata ttgtggaaaa tcggcaactc aatcctttac gcgactattt
     8161 cttgcgctac cctataaaag tccgtgaaca agtgcgtcta gtggtttcag atttctatac
     8221 accctatcgc acattggtga aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt
     8281 tcatattagc caacacattg gtcgggcttt taccaaccac cgaattcaag taatgaagac
     8341 ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa
     8401 gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt ccgtcatttc gagatcatct
     8461 gacagaagca gagatagtcg atcggctatt atcttatgat gattccttaa agcgaggcta
     8521 cgaagtctac caggattttc tttccgttat cagaagacaa gatgttcctg aattcgtcgc
     8581 gctcctaaaa gaagactaca aggagttgcc agagcactac caaccagtgt tcacgacctt
     8641 caagaaatac caaacagaaa tcaaacgagc gttgcgcgtt ccctattcca acggccctat
     8701 agaatgtttg aataaccata tcaaagtact aaaacgaatt gcctatggct atagaaactt
     8761 tcaaaactac cgagaacgaa tctttttata tcgaggaaaa tattttaaga aaactaagaa
     8821 tactacccaa ctgactaaag ccagaacaac aacacggcta gacaagatag ctgtctagcc
     8881 gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa cactcattga cgaagagcca
     8941 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
     9001 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
     9061 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
     9121 agtaaatgta gtaatctgtg ctttcttttt tctcaatact aataactaac ttatggggta
     9181 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
     9241 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
     9301 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
     9361 tttccttgcc atcaattcga acgatggcat atttgatagc atcttcagtt gtttcattct
     9421 ccttttctat agagaataca aataagggaa taaaagaggt aataatcaaa atgccaataa
     9481 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
     9541 taaacatatt agactttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
     9601 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
     9661 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
     9721 cgggattttt ttgtataatc atttgacact caattttagg taaactagct acccaataat
     9781 ttaatgattc aatactttta tagctacccg atgtttcaaa gatcgtcgaa aaatcactat
     9841 gttgggcaaa ccaaagaaac ccttttgctc ttgtaatatt gccaggaaaa tttttatccg
     9901 taagatgggc aaatttttgt gcgtcaagga gtaaccgatt tcgatagata aaacttgaga
     9961 taccatattc ttctgtttca ggaatatgat tttgatagcc aagctctaac tctttgaacc
    10021 agcccactga ataggaagtc tcatctatat taaaaagatt tgtatttaga attttcgata
    10081 agtctacttc acctttaatt gtcctaatta ttttggcatt ggactgtaac ttagcaatta
    10141 gaccgttaat tttgtaaatc gtcttggttg acactaagtc actattattt aaaagtaata
    10201 tgttacaaca ctcgaattga tcaattaaca agccacgaat atcctgttct tcttcaacta
    10261 cattaccttc agcttttctt tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac
    10321 gttttacatc cactactata ataatggcac caatctctaa tcgttgggtc aaatcaatgt
    10381 ctaaagggtt tttagctaat ataattgctt gaataattgg aattggtttg ctgattcctg
    10441 aagcttcaat aacaatttga ttcaagtcag aaaattgcac taattgatct agttcaacaa
    10501 tcaaatcttc gcgtaatgta caacaaatgc atccattcgg catcgaaaca agtttttctc
    10561 cagttctttt aaaacctttc ttgtcgacta ataccccatc aacatttact tcccctatgt
    10621 catttacaat gacagcaatt tcctttccgt gatcaccgta cagaatatga tttagaagta
    10681 tcgtttttcc tgaaccaaga tagccagtta agattgtaat aggtattttt ttcataggga
    10741 acctctttct ataccttgaa tttacacttt aagtgcaaca cataaaaatg agtcataatc
    10801 gatatccaac tagtataagt ttgtcgagaa cactcctaga aggaaaataa ttatgagcca
    10861 gtatcgtcat cttactatac tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt
    10921 ttctattagt ataattggca acgacattca gcatagcccg tttactattt tacatgaact
    10981 gagaagaaac aagaaaaaaa ctattcacct tccaaagcgg aaaaaaatta tgcttatcga
    11041 agaaaaaaat gcggacgtta tcacattcta aatgatccgt gagtttggtc aattgttcgt
    11101 catttatttg tcgattttca gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa
    11161 catatggact tttcaattag ttttatcacg atctaacgtg caatttgcaa tgatcttttc
    11221 gatacagaac ccctctcaca cggcaataaa ggcctgatcc gttcattgca ccatcgagga
    11281 aaaacaagac atacaaaaaa tcatgtcgag tctcgtggaa agatttgcct tactcattcg
    11341 atccatggcc gtccaaaatc cgcagatgat agaagtgatt ttggtcattg gaaagccgat
    11401 attgtagctg gcaaaactgg tggtacttgt ttagtgactt tgacagacag aaaatcatgc
    11461 ttccttttag caggaaagaa ggtccccaga aagaaagccg cttatgtgag cgagaaaatg
    11521 attgaacttt taagcagtat tccaaatgag gcggtcaaaa cgatcactcc agatagagga
    11581 aaagaattct ccaatcattt cgcagttaca gaagcattga ataatatgcc cttttatttt
    11641 ccagatcctc atgcccttgg caacaatgaa caaattaaaa tacgaataga ctcattcgag
    11701 aatacttgcc aaaatcgaaa gagatagcta cggtagataa ctcaacgata aatcagatga
    11761 ttgaaaaact gaataacaga cctagaaagt gtttagattg gagaacacct tatgaagtct
    11821 tctttgataa agtgttgcgc ttgatttgac aattcaagac ataataaaaa gcagatcaaa
    11881 tccaaataag ggaaaaatga tctactctgg tattatcaat taattggcca agctattatg
    11941 ccgttttgct aaacgtctac ttcttccagt attgaccttg ataaaacaac aatcaggatc
    12001 ccgttttaat actctatgaa ctgtgtcaat actaacatgt aaagttgcag caatatcagc
    12061 taatgtactc ccatttaatc tcatttgctt tgcaaatcga taacgccgat aagcatcaat
    12121 attcttggaa tccatagaca ataatacttt tcttaccatg tatttcctcc aaaaattgaa
    12181 ttttaatgag ccatcatttc ttgagcaatt tcatgtttag acaatgaagc tggatagaaa
    12241 gtaggccaat tatgcaattc attatagagt ttttcttgac tttctccacc aaagaaaata
    12301 tggaaatgct ctgccttact tggtgcaatt ccatgatcac taaattgaat atatttaaaa
    12361 cgaccagcac ctgcatcatc agtttcaaaa ttaaaacgaa caccacggtt tcctttttca
    12421 taattcaaaa ttttgtatcc aacatatttg tatgtgtatt gatgatgttc accgttgact
    12481 acaaaatcaa tggtgttgtc tttaatgttg atagtatcaa tatctgtctt atagccagta
    12541 gtataataat ctttgtactc ggcagctgtc atgtctttat tgatttttgc tttgtaatcg
    12601 aaaacttcat ccaaaattcc atcttttaac aacggataaa cagattgcca ttcgcctgta
    12661 taatcactca attcgcgatc cttcacatcg gcatctttaa agtacccatt agcaactgtc
    12721 ttttcatcct tcgcttcttt ttctggaact acctcttcac cagcaaccat agttgttttt
    12781 tctaacgctt ttagattgtc ttccattact gaaacataat cttcaccatt atccatttgt
    12841 tcttttgtaa gactttcaag aggattcagt acttccaatt ttatcccagc ttcgttggcc
    12901 aaagtttttg caattttatc attagcattc ttttcaaagt aaatataatt gatcttattt
    12961 tttttaacat actcttttaa ttcagctaat cgaccagaag atggctcttc ttcaggactt
    13021 agtccagcaa taggaacttg tttcaatcca taatccaaag ctaaatatcc aaaggctgcg
    13081 tgttgcgtca caaaactttt ttgtttcgct tctttaagac tagtggtata gtcagcatcc
    13141 aaccgtttca gtttagtcaa atacttttca gcatttgtct caaatacttt tgcttttttc
    13201 ggatacaatt taactaactg atccttgata tttgaaactt ccttaattgc cattttgggt
    13261 gaaacccacg tatgaggatc tagttcatga tggtgatctt cttctccgtg atcatgatct
    13321 tcttcttcac tacctggcaa taaaatcatg tcttttgttc cttctactac attaggccca
    13381 gctttcttcc aactttcttt agcttttggt acccatgatt ccatgttttc gttatgatat
    13441 acaaaaacat cagcatcatg agcttttgcc atatccttag ctgagggctc atagtcatga
    13501 ggttctgatc ccgctggaac cattaaatcc acgttcgctt catcgccgac gatgtttttg
    13561 gtgaaatcat acattggata gaatgttgtt acaatttcta attttccatc tttatcattc
    13621 gatacttttg catcattagt gctggagcaa gctcccaata atcctagagc tgctatccca
    13681 ataagtccta cactgatttt cttcattctt tttccttcct taattatgct aatttcttca
    13741 ttttatttcg tttatgttct ttgataattt ttaatgccct ttttctagtt ttaatattcg
    13801 ggcttttaag tcgtcgatat gcactagata tatccgtttt ctccattaga aattaccccc
    13861 aatcactact tatcattatt tgctttccgc cagtaattta tcaaaactct gtttgatcct
    13921 ttcttcatcc aacatttttc cgattagaat aatagtggtt tggtcttttt gtcgagtggt
    13981 gtattgaaaa ttaacctgtt cgtttacctc ttgcatggca actataaaat cctgctcttg
    14041 taaagcaatc aatcctttaa aacgatacag gctcccttgg ttggttaaaa tcagccagtc
    14101 aatccattgg agtaaaaaat ctttttttat gtcttgatct gaagttaaga gaattgattt
    14161 aaactcatgt acttgatggt gttgatgata gtatttgcca ttatagtgat tttctgatga
    14221 atcactttca aaaactaagg gatgattaaa ttttttcaaa ttataaaaat cctttttttg
    14281 aatcgtttca ctatgtgtga acattcgaat atcggataaa gggttaatac ttttgatttc
    14341 tctctgtaat ttttttagtg cagataaatt caagttttgt tttctagaca caaaaatgcg
    14401 atctgccatg acaagttgct taagggcttg tggttctctt tcaataagtt caaaactgtc
    14461 actatcaata acagtcaata aactatcgat atatactctt cctcttagtt gaggcgctgt
    14521 taaaagtgtt tgaatgattg gttggggatc tgctatacct gtagtttcaa tgatgatttg
    14581 tgaaattggt tgttgatgtt cttctattgt ttccagtaaa gaaatcaata ttgctaataa
    14641 atcactgcga agactgcaac acatacatcc attatttagt tgtaacaccc gttcctcgct
    14701 atgaactaat aatttgtgat caatcccaac ttcaccaaac tcattttcaa ttataataat
    14761 ttcttccctg gggtagggtg agacttgcaa ggcttgattg attagcgttg tttttccagc
    14821 ccctaaaaac ccagaaacga ttgtaactgg tatgcccatt ttgtcacctt tttaccactt
    14881 agctatttca cttctttaaa aattgcccgt ctgcgcaatt ttggcgaata cttttttaat
    14941 tctaaccgtt cagggttatt ccgtttattt ttgctggtga gatacagacg ttctccagtt
    15001 tcgacacatt ccaaaataat attttgacgc ataaatcttc ctccttcaat acttatcgat
    15061 cttcaagata tgcttgaatt tgttgtgcat cttcaaaagt taactcgcca gattccaatt
    15121 gtgaaactaa attttctttc atatcaacag gtatctccca ataaccatcg atacattgaa
    15181 ataactcctc gtcatactct tcaaaaattg gatagattct taaaaaatca tcaaatgata
    15241 agccttggtc ctcatagtaa tctttgacta atccattttc aattgcctgt tgggtcggta
    15301 aacccgtttc agtaataatt cctctctcta ccatcgaacg ataaaaagtg catttatcta
    15361 gcgattgctg ggtcgtttca agataatact ttaatccttc ttcaactaaa ggtgggtaaa
    15421 taatttccat tttgattccc tcctaaaagt tatatattaa actattttga agctccttta
    15481 attactttct ttccattata gtaccctttt aaagatacat gatggctacg tctataatca
    15541 ccattcgctt cgtcaaatga aatttctggt tttactacag tttgatgagt tcttcttaac
    15601 cgtttcttcg ccttagatgt ttttcttgct ggtactgcca tgatatttcc tccttttacc
    15661 aacttgcttt ttttactcca ggaatcaacc cttgatgggc aagttctcta aacttaatac
    15721 gggacatacc gaactttcgc atataccctc ttggacgacc atccgtctga tcacgaagcc
    15781 tcagacgatt cggatttgaa tctcttggca atttagcgag agcggtgcga tctcctgcgg
    15841 cttttaattc ctgacgttta tctgcatact tttcaatcat cgccatttgt ttctttgctt
    15901 tagcaatttt cgactttttt gccatatacc tttatactcc ttttattttg atttagacac
    15961 ctcaaatcgt aatcgttacg ttttgacact gatactttac caacaagagt tgctcttgtc
    16021 aacaataaat aacatgaaat aaaactcata tgaatagata actatttgaa aacgaattta
    16081 tcggtacata gagataatca aatcatttaa aagaatatgc agtttaggct ctgaaaaata
    16141 agaattcact tttattagtc caatcaaaca caaataccgt tttaagtagc ttttcctcta
    16201 cttaaaacgg caatatgaaa gaaactataa ctttttattc aatcttttat gtttaactgg
    16261 tttatcgcca attttttaaa actttgatta ttccttatat tataagaata gacattaagt
    16321 gaactaattc aatcatactg attctttcgt gtattcaaaa ttagatgctg tttaataaaa
    16381 ttattctttt tgaaaaagaa ttataactat atcccaaaaa ttgtaggtta atttaaagcc
    16441 tatttcatca tcgtgattga gcttcttttt catagacgaa tttctcagat agtttagttt
    16501 tctttatgag atttttgtat atttctgacc ttcgatacaa ctcattatga gtaccttgat
    16561 caactagttt tccttgatcc atcactacaa ttttatttac cttttcaata gacttcaagc
    16621 gatgcgatat aattagtact gtcttatctt gtattaaacg atttaaactt tcctgaatac
    16681 gtttttcatt ttccgcatct aaactggcag taatttcatc taaaattaaa attggcgcat
    16741 ttttcaaaaa agcccgtgct atggataggc gttgtcgctc tcctccagaa agtgaacttc
    16801 cgttttctcc gaccaatgta tgataaccgt caggtagttt ttcaataaag tcttcgcagt
    16861 tagctaaacg agctgctttt ttcacttcct catctgacgc ttgactattt ccaatacgta
    16921 tattttccaa aatacttgta ttaaacaaag ttacctcttg aaaaacaata gcgattttgc
    16981 taaacaaaga agctggagat acacgtttaa tatcataccc atcaattaaa atacagcctt
    17041 catcataatc atacagtcta gacactaatt tcaagatact agttttcccg gaaccactag
    17101 cacctactaa cgcagtcact tctccttgtt tagccgtaaa agagatatga tctaaaattg
    17161 gggtattgtt atcatatgag aaagagacat ctcgcaactc aatgtcaaaa gatttcagag
    17221 gactatctga tccctcttgg atactggtct ccttcatagc tctaattcgc tgtattttag
    17281 gagctaaata aaatatttca agcactgctt ctttcataga atcgaaggaa tcctttattt
    17341 ttattgcggc tagtaagtaa ccaacgacgt agagtatagt cacttcaccc gttaacagta
    17401 agtgaacacc tactagaatg actactgcta aactaacata tgagaaaata gaggataagg
    17461 ccatgactga aaaagtactt aattctacct ttaaatgaat cctttcactc tcttccattt
    17521 ttttgaaaag tctgtcttga acttttttag atagattaaa gctattaatc tcttgctgca
    17581 attctatagt ttcttgaaat tcttccgagt tttctctcaa agtatcgtaa tatttagtat
    17641 tggctttcgt ttgggatttc tttgataaca agattaagac aaaactaaat aacgttggca
    17701 aaataacagc taatcccatt ttgacattac caaccaaaag catcactgaa ataaaaggga
    17761 aaaacagagc cataccgccg gctttaggta ttgcatgact catcgcatgc tcaatacctt
    17821 caacgtcaga catgatagtt tgagataaat ctgataaatt atgttttgaa aaataggata
    17881 atggtaaatt tgacaaatcg tctgcaattt gcactcttaa atgcgcactt tctttataag
    17941 tactattata taagttttca tactccctat ctaacaacca atacatgaaa agcaaagtaa
    18001 caaacgatac ggcaaaataa agccaacggg gctttgtatt ttctaaaact aattcgtcta
    18061 tcagcatcat tataatgaat gctggtacca tattaatgca gtatacaaaa aaactagaaa
    18121 tagaagcttt agttaaagct tctgcgccct gatcacttaa agcaaatttg tttttataaa
    18181 aacttttcat tgtcaagcct ccaatcattt gcattctcat aattagtgac caattgttta
    18241 tacacttgat gtttagccat taattcatta tgatttccac gttctactat tttcccttct
    18301 gacaagacaa gaatttcatc tacagattta atgctggata atcgatgagc tatcataata
    18361 actgtttttt cttttatcag atgttgaaaa gccttttgta gttcatactc attatctgga
    18421 tcaatagatg cactggcttc atccataata acaatctttg catcttttaa aattgctctg
    18481 gcaatagcta tacgttgttt ttctcctcct gacagataaa ctcctttact accaattaag
    18541 gtatgctctc tttcgggaaa tttttctaaa atcgattcgc aaccggctaa tcttagcgcg
    18601 tcaaatactt tacttgctgg cgcttgagga tttgctatag aaacgttatc gtaaatactc
    18661 tgattgaata gtttcgtatc ttgaaagaca aaagcaattg tgttaataat tgtgtctttt
    18721 gaatagtgat ttaaaggctt accaccaatt ttaatctgtc ccttatccac attgtagaaa
    18781 cctgaaatta gtttagctat cgtactcttt ccgcttcctg attttcctac taatgcataa
    18841 attctttttt gttctaaaac taagttgaaa ttttctaaaa cacactcttc tttgtatgaa
    18901 aagctgacat tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt cccatatttt
    18961 agttggtctt gcttcatttc taaataaagg gagtctagtg tttcgactgc atatcttgct
    19021 tcaaacaagt gcatacttac atacattatt ctcatgaaag aaacaaaaat aattccactt
    19081 aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg gagtggtaag agaagttata
    19141 aaaaaggtta taggaactat caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga
    19201 acaaaaggtg ttttcccact caaagaatac ttatatgcaa acttagcata atcgttaatt
    19261 gctttattca aggctttaaa tgatgttact ttagcaccaa atatctttat aacttgaatt
    19321 ccgcggatat attctaccgt ttcagagctt aacacatcta acgattcttg atatttttga
    19381 ataaaatttg tattctctgt cattttcttt aacaagaata caccaattac taacatggcg
    19441 attagaacca atccaatttt aaagttaatg aaaaagccta aggcaactga taaaacaggt
    19501 gttactatgg cttgagcact atctggaatc aagtgagcaa ttgccatatg tgttttagcc
    19561 gcgttgtcat caattgtctt tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa
    19621 cttgcttctg ttaaaccgct aattcccttt tttctcaaat tagtctctaa gcgaaaccca
    19681 aggatatgag aaaataagcc agaaaaaata taaaataaag ctcctaaagt taaccaagca
    19741 acagtttgaa ccgcataact cttagctaaa tattcatctc ctacaataat tagagactga
    19801 agaaatttgt aaatactgta atatccatag actgttaata cgacagaaaa atcagaaaat
    19861 aaagtggcta accatccgta gtatttcatt ttagggacat aactaaataa ttttttgtat
    19921 actttcattt tttcctcccc cgcattcaat ttcttctctt attcgtttac aactgttaat
    19981 gctttccgta atagcgtaaa ttagagatgt acaaacagat aaaaggtaaa ctacgttcta
    20041 ttttttgtat ttttttaaga cttttcttcc aatcaataca ccaatcatag ataaacttgc
    20101 tacaactaac actgaaatga ttccccatat acctaccgca tagtggatca ttgcatccat
    20161 tgttgcagca gattgtcctt tatctatcca atcttgacga aaacgatctg taaagaacca
    20221 tataggaatc cactgtccgg cagcactgcc taattgcatt agagcccaag acgcaacttg
    20281 aggaattgct cgatcatatc caaatacaga tagaataata tcagctaaga tcgccatact
    20341 aatcgaactg ataaaccaag gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa
    20401 aacaattgtg taaatagtaa aaattccttt tctagggtag ttatagctaa tataaacaaa
    20461 tatgattcca gataataatc caaaaatccc gggggatatg ctgtgaccaa acgccccacc
    20521 aatactacta accattattg aaaaaaagta aattactagg gaaataactg tagctaaacc
    20581 aataaaaata taatcttttg ttttcatata tattctcctc aattctattt cgttatgtca
    20641 ttaaaaatct gttctatcaa agttgaatct tctactctaa gttttatatc tttttttatt
    20701 ccaccatttt ttaaataaat aattctattg catgttttat aaagaaattc cggatcatga
    20761 gtagctataa ctactgcacc cttttctttt aagtctttaa taaggtcagc aatcctttcc
    20821 atattaatat aatccaaccc acttgtaggt tcatcaaata gaaataaatt agtttttgac
    20881 aatgaagcta tagaaaccaa taatctttgt ttttctccac cggataaatc aaacggtaat
    20941 tgatctttca ttttataaag ttccattttt tttaaaattt tttcttgctg atctttacta
    21001 actatttcat tccccagggc taactcgctc ctcactgatt ctgtgaaaaa ttggtaatcc
    21061 atttcttgca ttactaaaaa cggcgatgac aaattttttt tattttcgat tatctttcct
    21121 ttggtagggc gaataatctt catgagtatt cttagtaagg ttgttttccc cacaccgttt
    21181 ttccctacca aaccaataac atctccaact ttgacatcaa aatttatgtc ttttagaata
    21241 ttttcataag taatttcttc aacttttaat acatctgcac ttaccttttc gatataggga
    21301 acttcaatcg aactattaaa aatgtcaaat ttccttaatg cgttattgtt ttcagttttt
    21361 aacaactgaa tagttgtagt ttttacagtt ctatttttaa caaatagaac ttgatcaatt
    21421 aattctttca aatagtaaaa acgatgttca gctataacaa tagtaaatcc tttatttttt
    21481 aaatcacaaa gtaagtcagc aagtttctta gtcgctttga agtccaaatt tgaagacggt
    21541 tcatccaata taattaattt agagtttagc gataaactac atccaataga aattttctgc
    21601 cgttcaccac ttgatagcga aaaaatgttt ttatctaata tatttgaaag atctaataat
    21661 tcagctaatt cattgattct tttatccata tgctcttttg taaatccaaa gttttccatc
    21721 gaaaatacta actcactcgt tgtatttgtt gtaaagaatt gtcctctagg atcttgactt
    21781 acgtagccaa tattttttga tatactttct tgtgttaagg attgtagcga gttattttca
    21841 aataaaattt ccccaaccaa ttttccgtca taaagtcttg gaataagtcc attcattaac
    21901 tttaatagtg tggatttccc actcccagaa gaaccagtaa tcaatgtaag actgcctttt
    21961 tttattgaaa aattaaggtc aacaagcgct tgttccttaa catccttata tgaaaaagaa
    22021 acattgttaa attcaaacaa ttctttcacc tcaaatcaaa atacttaaga aaaaaatagt
    22081 aacggaaaac aggatgatat agtctaataa gctcatgttt gttttatagt aagaacttct
    22141 tttcacagga gattctatcc cctttgttat tgcattagcg gtcatttctt cagcaatcat
    22201 agttgcacgt ataagaacgg gaactaacag aaattccaaa tattgcagag gctgaaaaaa
    22261 agacaagcga atgtttctca tttttaaaga ttctttaatc attcttattt cagaacgata
    22321 agtcggaaag aatctgatca ctactgtaat acttaacatt atttttttcg gtaaatgtat
    22381 tttttctaaa gaggataata attcattgct agtatagaca ctaaccaaca accatgagat
    22441 cattacgacc ggcaacattc gaaatacaat atataaggat gtcgcaaaaa atatagtaaa
    22501 aaactctatc ttgatagcat caataatttt cattactaaa aacagaactg aataaatcaa
    22561 gatgaaataa atacatgtct ttattttacg aaacatcaat actaaaactg tagcaataat
    22621 gaaacacccc acttgaaata tatttccctt tcctaaaaaa ataatgaccg gaaaaaaaat
    22681 atttaaagta atcaaggtta acggattgat ttttgacact ctttcacctc caaaaaataa
    22741 gatcgcactg ttatcttaag ctgataaaaa tgcaccgtta ttcagataac agtgcgatct
    22801 tatcaaaaag cataactgag aacaattctc aatgcaagct ttttttaatt aactcagtaa
    22861 ttcaatccac ccaggaacag aaaattgata caatcttctg gttaatagca tagtcttttc
    22921 ttttgaatag ctatgcttca atatatcata gaatgtagcg tagaatgagt aaatgtagaa
    22981 gtggatttct tcttcattaa agactaaatt ttgattgatc attccgatag tctttaactt
    23041 atctagacaa cagactgtct ttttcgtcac aaatgatgta attttttcaa caatatcttg
    23101 atatttggtt ccagctgatt ggaataaaat caaatcgaat atacgtttat tctcaaaaat
    23161 ataattaata aatggatcta attcattgaa gttctctctc caacttttct ctatatctat
    23221 attttctaag tttaagaggt atttggtaaa ctcttcatta tacatattaa taagcgcatt
    23281 aatatgcggc tgaataattt cttcaaataa cgcttcctta gaagtaaaat gtttataaaa
    23341 agcacccgta gttacgcctg cggtcaggca cagttgcctt atcttagttt gctgaaatcc
    23401 ttctttttca aaatggctta gtccactttc tattaacttt tggtgagtta taatataatc
    23461 tctggtttct atgggtactt cctcctaata agatgaatta actttattat cttacacagg
    23521 acttgaagtt tcaatcgtta acgtttatta ctgaactcat tccttagagc taaactaatg
    23581 agaaagagca gaataactga tacaaaaagc aatcctacaa gaattaatag actcatctaa
    23641 atcattcacc ttttctaaaa taatccgaga aagatttaaa aaaattatat ccagctatta
    23701 ggataagtcc tatcataatc actccaatag ccattttctt atcctcccta aaaataaagt
    23761 cttttactta aaaccttttt tgtaatcgtc aataaatttt ttccctttat cagttaattg
    23821 aaatcctata tctaaatagt cctcatatct aagtttagcc agcatctcac ttacagatgc
    23881 tggactaaca cttagaactt ttgctaaatc attatttttt aatttctttt ttctttttga
    23941 taattcctga attgctagta aataaattag tatagtagtc gttttaattt gtaccatacc
    24001 taccctccaa attatttcga aaggccttca gcaattttat ccaggttcca tttcatcatc
    24061 gcatagtaac tatcaccatc ttgtcctttt tctgcaattg aatcagtaaa aatcgttgaa
    24121 tagataggaa tattggtatc ttttgatact gttttcattg gtctatcatc cacactgctt
    24181 tctacgaata aggagggaac ttttgttgtg cgtaattttt caactaagtg ttttatttga
    24241 tctggtgttc cttcttcttc agtgttgatt tcccaaatgt aagcagaagg cacattatac
    24301 gctttcgaga aatatttaaa gcatccttca cttgtaacaa tcattttttt atcattcgga
    24361 attgaagcaa atttagattt agcttctttg tctagagaat ccaatttttc aatatactta
    24421 tctagatttt ctttatagaa ttttttatta tcaggatctt tttccgctaa ctgtttttca
    24481 atatttttag cgtaaataat accgttttct aaatttaacc aagcatgagg atcttccttc
    24541 cctttctcac tctgaccctc taagtaaata acatctatgc catcacttgc tgcaaaatag
    24601 tctttgtttt cctctttgtt cgcattttta actaattttg taaaccaagc atttcctcca
    24661 gtttccaagt taacaccgtt ataaaaaatc aaatctgctt ttgaagtttt ttgaacatct
    24721 tcaggcaaag gttcatattc gtggggatct ttcccaatag gtacgatact gtgtaaatca
    24781 attttatctt ttgctatatt ttcggtaata tctgctaaaa tcgaattagt aactactact
    24841 tttaattttt cgttcgaagc cactttatct ttgtctttac tatttattga acatgccccg
    24901 aatataaaaa gagcaatcaa aatccccaga cttcccagta ctgctttcca ttttctcatc
    24961 ataacgatct ctccttcttt cttaaaaatt tttgttttgg tgagattaga aaactaatta
    25021 caaataagaa tgccgctgtt aaaacaatac ttgatcctgc tgctatgtta aaactataac
    25081 caataaacaa tccaagcaat gatgaacttg ctccgactgt tgcagaaata aatatcatag
    25141 ttttcaagct atttgcatat aaatatgctg tagctgctgg tgtaattaac attgcaacaa
    25201 ttaatatagt tcccacactt tgcatagctg ttactgctac taaagttaat agaaccatca
    25261 atagataatg ataaaaactg acattcatcc ctattgcttt tgccattaat ggatcaaaag
    25321 aagtgattaa taattcttta aaaaatagaa ctactataac caacactaaa accgaaacgc
    25381 caattgtaat ccacttatca atatcttgta cagctaaaat atttccaaat aaaatatgaa
    25441 ataaatccgt tgagctattt gcaactccta taaggataac tcctagtgct aaaaaagaac
    25501 tgaaagtaat accaattgct gtatctcctt ttattacact attgcttttt ataaaagtaa
    25561 taatgataga agctaataga ccaaatacaa tcgcaccaat aaaaaaatta atacctaaaa
    25621 tataagaaat tgctactcct ggcaaaacag catgagaaat agcatcaccc ataagagaca
    25681 ttcctcttaa aataataaaa caacctactg ttccagaaac aatccctata actacagatg
    25741 taattagagc attctgtaaa aagtgaaagt catacagacc attaataaaa ttttgaagca
    25801 tattattccc cctctccaat aaatatagta tctccatagg ctttttttaa attttcttca
    25861 ttaaatacag attctacact tccgtgagca attagatttc gattcaaaag aataatatta
    25921 tcaaaatact tctttacctt gcttaaatca tgatgcacaa ttagcactgt ttttccctga
    25981 tgtttcaatt tttttaacgt atccataatt attcgttcac taactaagtc tatccctaca
    26041 aatggttcat ctaaaaatat gaaatccgca ttttgagcaa gacatcttgc taaaagtaca
    26101 cgttgaaatt gtcctccaga taattctcct atttgatgat tagaatattt tcccatatcg
    26161 accttttcca aagcctttga aactctttgc cattctgcat ttttaattct ttgaaaaact
    26221 ttcattccgg catatgttcc catagataca cattctttta cttttattgg aaaagtaaaa
    26281 tcaatatcag ctttttgctc tacatatgca atcttcacca gttttttatt gatatcttca
    26341 ttattaatgg agacctctcc ctgatgaggg ataatattta acattgcttt aagcaatgtg
    26401 gattttccag caccatttgg accaataata cctgttaaag caccttcttg tatttttaaa
    26461 gaaatctcgt tcaatgctaa aaaatcgtta taacaaacat taagcttttt cacttctaac
    26521 ataactttct ccttctaaat gataatgatt atcacgatca tatggtatac aaaaattata
    26581 ttttagtcaa ccctaaattt tatagtttag atttttttct gttgctgaag attagaatga
    26641 caaaactatt tgaaactacg cgtatagtaa gtagctttca aaatatataa aaaaggagcg
    26701 ttctcaaatg agaacatgct cctaaaacta ctttattggc gtatggaaaa attatacttg
    26761 aagtggatag cgggttgaat aagttgataa gcatcaaaac atttataaaa attattcaag
    26821 actatgtctg ttccatatac aaatatgaaa atcagtaaac tccatttaac ttttttcgta
    26881 gtgtgtcttt ttatacatgt ttatcgttat taatagtgaa taggaaaaaa actatttttg
    26941 atcattgaca attaaataca atttttgaat aacgatccct gctattcgag ctagaaactc
    27001 aagtacgcca tgacctgatt agcgacaaat acttagagat ttctttttgt tctgggaaaa
    27061 caaaatgcca tgacaatagc agcaaataat gatacttccg taaggctcgc cgtaaactgg
    27121 ggagaggtga aattcctatg tgcttaccca aagcaattca aaaatttcat aactactatt
    27181 ttaggagatg attttataga atccagagtc tacctgaata aaaaacaaac ctgtgcattt
    27241 ctaggaatcg ataataatac tctagataag tggattcgta tatataattt cccagtaata
    27301 agagttggtg gtgtagttcg ctttgacgta gaagatatta aagactttat gaatacattt
    27361 aaaataactt gaaattttct tctcgccgca tatactaaaa ttagtgaaat atactgcctt
    27421 acttttttta aggagtgagg attttgccta caaagctttc aaatgggaaa tataaaacta
    27481 atctgagata tccaaaaagg tttaaagaaa taacaggaat tgcatcagaa aagtatcaaa
    27541 aaacatttcc aaataggcaa ttggcgatta aagccgaaaa tgatatgaag aagaaaatag
    27601 aaaaagtttt gagagaggag aatgcaaact cattagaatt aaaaggaaaa ataacgttta
    27661 aaaaatttta cgaatctaaa tggcttccta gatatgaatt aggtcaaaca attagatcaa
    27721 acaggcctcc tagtgatata accattagta atacaaaaga tatatttcgg ttgcatattt
    27781 tacctatgtt tggtgaatac gcaatgaatt atctaaatat caatactgag ataatatcag
    27841 atgaattaac caaaaaatcg aaagaatatg caaatataaa aatcataaaa ggatatgttc
    27901 gttccatgtt cgatattgca gaaatactaa attatatcga atttaatcga actactaaaa
    27961 ttattcaaag tatcactgca cctaaaaaaa atgctttaga agaaaaaaga atacaagaag
    28021 gtaagcaagc actatcatca aaagaactta ctaattggat agaagcagta aatgatgatt
    28081 ttaataatca tttacttact tttcatgatt acacactttt tatgattacc ctgtatcttg
    28141 gtgatagaaa aagtgagact tacgctttgc aatggaaata tattgatttt gaaaaacaaa
    28201 cggtccgtct taaacatact ttagataaat atcaaagaaa aaagtttaca aaaggtcgaa
    28261 aagataccgt cattcaagta ccagaagttg taatgacatt gctaagtgaa tggaaatcgg
    28321 tacaagctga tcaattgttg aaattaaaaa ttaagcaaac gttagatcag tatctcttta
    28381 cttatactaa accatctgga gaagtaaatt gtcctgttca tgccgattac ctaaactatc
    28441 gaatcaattc aattaaaaga aggcatcctg acttagttca tttatctcca cacaaactcc
    28501 gtcatactta tgcaactata gctagacaag gtggagcaga tatgaatcaa atttcaaatg
    28561 ctttaacaca ctcagatatt tctacaacaa agatatatgt taacacacca gatatagttg
    28621 ataaggctgt ttttgaagct tttcagagag gattgaataa gtgtgattga tatgtgttct
    28681 taaatgtgtt cttaaaaaca aaagaagcac tttccaaata attgaaaagt gccagaaacg
    28741 ttgatatata aaggatatta acgttttgag aattgtgaag ctttacgtgc tttcttaaga
    28801 cctggttttt tacgttcgac catacgtgcg tcacgagtaa gtaagccagc acgtttaaga
    28861 gctaaacgga agtcaggatc aacttctagc aatgcacgag cgataccatg acggatagct
    28921 cctgattgtc cagcgtatcc tccgccgttt acgttaacaa atacgtcata tgcgcctttt
    28981 gtttcagtaa cagcgaaagg ttgattgatt acttcacgca agtcagcatg tggaatgtat
    29041 tcttcaacat cttttttatt tacagtaatt ttgccagttc ctggtactag acgtacgcga
    29101 gcaactgcgt ttttacgacg gcctgtgccg atatattgaa cttgtgccaa tgaaattccc
    29161 tcctattaga ttaagtttgt gatgtctaaa acttctggtt gttgtgctgc atgtggatgt
    29221 tctgctccac cgtatacgtt caattttgta aattgtttgc ggcctaaagt gtttttagga
    29281 agcatacctt ttacagaagt ttcgatcaaa cgacgagaat ttttagcacg caattcgcca
    29341 gcagtgattg atttcaatcc tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc
    29401 gcttttttac ctgttaattt cactttgtct gcattgatga cgatgacgaa atcgccagta
    29461 tccaaatgag gtgtgaaagt tggtttattt ttaccacgta gtacagatgc aacaactgtt
    29521 gaaagacgtc ccaatggaac atcagttgcg tctactacat accatttacg ttctacttcg
    29581 cctggtttgg ccatatatgt tgtacgcacg ttattttcct ccaatttaag ttctgatgtt
    29641 tgacatacac ttcgagtttc cggggctctt tgtggggcaa acaataccat ttaacatatt
    29701 acccgctaat ctagtcaatg tcaacgtttt tctgcttgat tgaaaaattt atatgaaact
    29761 ttacttcttt tctttctttt atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa
    29821 gaggctgtga caaaagtctt gtcacaaccc cttattttga ataaacggtg ttctcctgcc
    29881 aactcctcgg tcacaagtca caatatccat aaaacataag aagctgtttt ttaaaattgt
    29941 ttcttgtgac tcggagctga acggcaggct
//
LOCUS       DACECR010000026        29200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.026, whole genome shotgun sequence.
ACCESSION   DACECR010000026 DACECR010000000
VERSION     DACECR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.026"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1475)
                     /locus_tag="IWO17_001082"
     CDS             complement(<1..1475)
                     /locus_tag="IWO17_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289974.1"
                     /translation="MKIIKKKIAGRKINWLALSLLALMFIVIFTGNVAHASIFNWFDD
                     TESQTEFLNNPVYQPYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFVESAGLNKVYQSVLSTIVVGLMILSLVIIGYKMVTGKGTVDLKSVGINVLMSIVLI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     MIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENSTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISIGLIALAIAYVFSAFVIITAILELAFKRV
                     LGVLVFATDLETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRA
                     KGSGNGNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYA"
     gene            complement(1462..1680)
                     /locus_tag="IWO17_001083"
     CDS             complement(1462..1680)
                     /locus_tag="IWO17_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0289975.1"
                     /translation="MIENRLIDQICCLDQNVDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQEMAQKLSESLKKWAEGGNNENN"
     gene            complement(1673..2638)
                     /locus_tag="IWO17_001084"
     CDS             complement(1673..2638)
                     /locus_tag="IWO17_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAQ0289976.1"
                     /translation="MKTLFDYILTLTGGSVEFMAEKRMLSKTFLNNASFMKMSDSAKL
                     LYVYLNVNADDDGIVEAYPYVRMLGTPDDDVKILLAKEFLYMLNEDLLVFLPHWLQNQ
                     TLRTTRYTPSIYRFKLLNSNPDFFTRLKQISIEKILKAGQTEMLRNDLIEKWYPVGLP
                     KDDHGHAQVRLGQVSSDQSRSGKVSLGEIREDQSINLSDDMEVEIMRQKIKNQIGYNA
                     LKSNPCYKRIVEDIVNIIQSIYSEKSKTYRINREEKSANEVIEKVEQLNQFDIESAID
                     SFNAIDHEIHNLNLYWITTLYNCKNTVSSRAYNNFVTQTREVLYD"
     gene            complement(2828..3112)
                     /locus_tag="IWO17_001085"
     CDS             complement(2828..3112)
                     /locus_tag="IWO17_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0289977.1"
                     /translation="MDVSSVQVILPPDLDQSLRKYFYQVAKEAVDQVRIETSLSKEIF
                     RLGEACEYLSCSRSTLKKFINQRGLKVSIIGDCQFIRKEELARFIKEYEI"
     gene            complement(3125..3418)
                     /locus_tag="IWO17_001086"
     CDS             complement(3125..3418)
                     /locus_tag="IWO17_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0289978.1"
                     /translation="MTDNEINAILSLVKTKKDEVVRLDSPVRKYRRFCGYRQKELAEL
                     LEISELTYRNKEKGRVPFKDYEIERIVKLFHKDGIHLDIVDIFFSDLKTKKDE"
     gene            3487..4251
                     /locus_tag="IWO17_001087"
     CDS             3487..4251
                     /locus_tag="IWO17_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0289979.1"
                     /translation="MNLNKTSVGNRIRLVRKELGLSMKEFGKKITPTAADSIVSRWER
                     GISLPNNDRLTQIAKIAHKDINWLLWGTFKEFISSLLDKEGYTNFLNDFPETVDTIYN
                     DLMPISENLQFPDYENSILRLFNKIYLPIFNDYCLDIIELDLEDFINKRHSISSDKFK
                     NRFLSSFHEGVSNSGYKYGDTDLIYKYGIALLEKFDDSYKLNNPYESIDAYFSAMTVN
                     TEDTEKLIYTICKSLGIPFQKGSNTAQILSKNHNNF"
     gene            4302..5447
                     /locus_tag="IWO17_001088"
     CDS             4302..5447
                     /locus_tag="IWO17_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0289980.1"
                     /translation="MATIKQYEKKDGTKAWLFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKKETNETFKEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTASRIFKYAMTLGIITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLESLFQYLESKRSTYRADYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(5532..5837)
                     /locus_tag="IWO17_001089"
     CDS             complement(5532..5837)
                     /locus_tag="IWO17_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ0289981.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(5863..6294)
                     /gene="ruvX"
                     /locus_tag="IWO17_001090"
     CDS             complement(5863..6294)
                     /gene="ruvX"
                     /locus_tag="IWO17_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ0289982.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(6291..6557)
                     /locus_tag="IWO17_001091"
     CDS             complement(6291..6557)
                     /locus_tag="IWO17_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ0289983.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6811..7536)
                     /locus_tag="IWO17_001092"
     CDS             complement(6811..7536)
                     /locus_tag="IWO17_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ0289984.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7902..7972)
                     /locus_tag="IWO17_001093"
     tRNA            complement(7902..7972)
                     /locus_tag="IWO17_001093"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7938..7940),aa:Gly,seq:ccc)
     gene            8089..8841
                     /locus_tag="IWO17_001094"
     CDS             8089..8841
                     /locus_tag="IWO17_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ0289985.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8857..9336
                     /locus_tag="IWO17_001095"
     CDS             8857..9336
                     /locus_tag="IWO17_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ0289986.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(9587..11167)
                     /locus_tag="IWO17_001096"
     CDS             complement(9587..11167)
                     /locus_tag="IWO17_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ0289987.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(11160..11903)
                     /locus_tag="IWO17_001097"
     CDS             complement(11160..11903)
                     /locus_tag="IWO17_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0289988.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(12079..12855)
                     /locus_tag="IWO17_001098"
     CDS             complement(12079..12855)
                     /locus_tag="IWO17_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0289989.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(13103..13939)
                     /locus_tag="IWO17_001099"
     CDS             complement(13103..13939)
                     /locus_tag="IWO17_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ0289990.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13950..14879)
                     /locus_tag="IWO17_001100"
     CDS             complement(13950..14879)
                     /locus_tag="IWO17_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ0289991.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15071..15424
                     /locus_tag="IWO17_001101"
     CDS             15071..15424
                     /locus_tag="IWO17_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0289992.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(15498..16154)
                     /locus_tag="IWO17_001102"
     CDS             complement(15498..16154)
                     /locus_tag="IWO17_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0289993.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            16380..18314
                     /locus_tag="IWO17_001103"
     CDS             16380..18314
                     /locus_tag="IWO17_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0289994.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(18400..19623)
                     /locus_tag="IWO17_001104"
     CDS             complement(18400..19623)
                     /locus_tag="IWO17_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ0289995.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19617..21515)
                     /locus_tag="IWO17_001105"
     CDS             complement(19617..21515)
                     /locus_tag="IWO17_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ0289996.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(21509..22627)
                     /locus_tag="IWO17_001106"
     CDS             complement(21509..22627)
                     /locus_tag="IWO17_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0289997.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22710..23543)
                     /locus_tag="IWO17_001107"
     CDS             complement(22710..23543)
                     /locus_tag="IWO17_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0289998.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(23536..24453)
                     /locus_tag="IWO17_001108"
     CDS             complement(23536..24453)
                     /locus_tag="IWO17_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0289999.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24680..25804)
                     /locus_tag="IWO17_001109"
     CDS             complement(24680..25804)
                     /locus_tag="IWO17_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0290000.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25987..26895
                     /locus_tag="IWO17_001110"
     CDS             25987..26895
                     /locus_tag="IWO17_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0290001.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            27036..28649
                     /locus_tag="IWO17_001111"
     CDS             27036..28649
                     /locus_tag="IWO17_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ0290002.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         9219 a         5702 c         4743 g         9536 t
ORIGIN      
        1 tgggcatatc ccaaagctct tccagttttg ccagcttttt ttgatagagt ttcgccgaaa
       61 ttcttttctg gttttcgatc ttctccttct ccgctgtctg atccatttcc atttccgctt
      121 cctttagcac gattaaatcc atttgatcct tttctaaaca gcattgcgcc cataccaaaa
      181 gcagaagcaa gttgcccata accttctttg agtccaatat caacaccaaa gtatctcatg
      241 actgttccac tgcctttgat taaaacaaaa acggcacaga tataagcaat tgtttttata
      301 aaaatatttg ttgagatacc ttgtcctgag ttcagaaaat tgataaacat agcaaacatg
      361 gatagtccaa atccttgaaa gcctacagtc aaatagcact gtagaatatc tgatagaaca
      421 acttttgaac gctgtcctgt ttctaagtca gtagcaaata ctaaaactcc gagtacacgt
      481 ttaaatgcta actcgagtat tgcagtaata atcacaaagg cgctaaaaac ataggctatt
      541 gctaaagcta tcagcccaat tgaaatgccc catagattcg cttgatagcg ataatatcct
      601 gatttgatac catcaacaaa ggttgacaaa aaattatctg aaaatttagt agcaacaaat
      661 tcattatcag aattttcaac taattcataa cgcaggttat cagctgttga gttctctttt
      721 tccattttat caattacatt atctgtcaat acttgcgtaa gatccgtttt ccaaaaattt
      781 tcttttgtta gattgttccg atcattcgtt ttatcgatca tggaatattg atccgtttta
      841 ttgatataag ctaaatcagt aaccccttgt tcaatcagcg accatgccac accgccatct
      901 tgattgttaa tactagttgc gtcattataa agagtctttg caaacattat tcctgaaagt
      961 ttttctgttg atccaatcaa agtcggcatt aaaagaatta atactatcga cattaaaaca
     1021 ttgattccaa cagattttag atcaaccgtt ccttttccag tcaccatttt ataaccaata
     1081 atcactagtg aaagtatcat taagccaaca acaatagtgc ttaaaactga ctgatagact
     1141 ttgttcaatc cagcactttc cacaaagcta aacaaatcta acacttcagg aatcatattt
     1201 tgaataccat ctacaattgc aaacatcgat tttataacag accaccctat ccacaatcca
     1261 aattgactgt aaaaatttcc gttatatttt tttaaatacg gttgatagac aggattattc
     1321 aaaaattcag tttgactttc cgtatcatcg aaccaattaa aaatgcttgc atgagcaaca
     1381 ttaccggtga aaataacgat aaacattaaa gcaagtaagc tcagagctaa ccagtttatt
     1441 ttcctaccag caatcttttt tttaattatt ttcattgtta cctccctcag cccatttttt
     1501 taggctctca gaaagcttct gagccatttc ttgattaatt atgatatgag ttacacaatt
     1561 gtcattttca gctcttaaat cgagaaccac ggtgttaaag cagatactct cgattaattc
     1621 gagagtatct acattttgat cgagacagca gatttggtct attagacgat tttcaatcat
     1681 ataacacctc tcttgtttga gtaacaaagt tattatatgc tctggaactt acagtatttt
     1741 tgcagttata taaagttgta atccagtaaa gattgagatt gtgtatctca tgatcaattg
     1801 cgttaaatga atcaatagca gattcaatat caaattgatt gagttgttct actttttcta
     1861 tcacttcatt tgcagatttt tcttctctat ttatcctgta tgttttgctc ttttcagagt
     1921 atattgattg aattatatta acaatatctt caacaattct tttgtaacaa ggattggatt
     1981 ttagcgcatt atatccaatt tgattcttta ttttttgtct cataatttcg acttccatgt
     2041 catccgatag attgatagat tgatcttccc taatctcacc taaactaacc ttacctgacc
     2101 tactctgatc tgaactaacc tgacctaatc taacctgggc atgcccatgg tcgtcctttg
     2161 gtagaccaac gggataccat ttttcaatca aatcatttcg aagcatttcc gtttgacctg
     2221 cttttaaaat tttttcaata ctaatttgtt ttaatcttgt aaaaaagtct ggattactat
     2281 ttaataattt aaatcgatat atagagggtg tatatcttgt agttcttaac gtttgatttt
     2341 gcagccaatg gggaagaaat actaataaat cttcattcag catatagaga aattctttag
     2401 ctaaaagaat tttcacgtca tcatcagggg ttccaagcat ccgtacatat ggatatgctt
     2461 cgacaatccc atcatcgtct gcgtttacat tcagatatac atataataat tttgctgaat
     2521 ccgacatctt cataaaactc gcattattta aaaaagtttt tgacaacata cgtttttctg
     2581 ccataaattc cacacttcct cctgtcaatg ttaaaatgta gtcaaataaa gttttcacag
     2641 aactttatta ttgagtgttc ttccggtctg caaactgtaa ggagcactct ttttttgtat
     2701 attcaccttt tattaaatca aatagaaaat aaatcttgat ttcttttaaa ctcaactttg
     2761 cttcttcatc atcgttcaaa caaacacttc ctttttttca tatcaaaagc acaccacgca
     2821 acttttttta tatttcatat tctttgataa atcttgctaa ctcttctttt ctgataaatt
     2881 gacagtctcc gatgatactc acctttaaac cacgctgatt aatgaatttt tttagtgtac
     2941 ttctactaca actcaaatat tcacacgctt ctccaagccg gaaaatttct ttgcttaaag
     3001 atgtctcaat acgaacttga tcaacagctt ccttagcaac ttgataaaaa tatttacgta
     3061 atgattgatc aagatctggc ggtagaataa cttgtacact cgatacatcc atgatgccca
     3121 ctcctcattc gtcttttttc gtcttcaaat cactaaaaaa aatatccaca atatctaaat
     3181 gaattccatc tttatgaaat agctttacga ttctttctat ttcataatct ttaaacggca
     3241 cccttccttt ctctttgtta cgatacgtta gttccgatat ttctaataac tccgcaagtt
     3301 ctttttgtct atatccacag aacctgcggt attttctaac tggagagtcc aaccttacca
     3361 cctcgtcttt tttcgtcttt accaacgata gaatagcatt tatttcattg tccgtcaata
     3421 cttacccgtc gtttttcgta gattccattc cgtttttgtg ttacattact gtcggaggga
     3481 tataatatga accttaataa gacatcagta ggaaaccgta ttcgtttagt tcgtaaagaa
     3541 ctcgggcttt ccatgaaaga gttcggaaaa aagataactc ctaccgccgc agatagtatt
     3601 gtttcgcgtt gggagcgagg gattagctta cctaataacg acaggctaac tcaaattgct
     3661 aaaattgctc ataaagatat taactggtta ttatggggaa cttttaaaga atttatttca
     3721 tcattgttag ataaagaagg atacacaaat tttttgaatg attttcctga aacagtagat
     3781 actatttata atgatttaat gcctatttcg gaaaatttac agtttcctga ttacgaaaat
     3841 tctattttac gactattcaa taagatatac ttacctatat tcaatgatta ctgtctagac
     3901 attattgaac tggatttgga ggactttata aataagagac attccatctc ctctgacaag
     3961 tttaaaaata ggtttttatc ctcctttcat gaaggagtat ctaacagcgg atataaatac
     4021 ggcgatacgg atttaattta taaatatgga attgcattac ttgagaaatt tgacgattct
     4081 tataaattaa ataatccata tgagtcaata gatgcgtatt tttctgccat gactgtaaac
     4141 actgaagata ccgaaaaact tatatatacc atttgtaaat cgcttggcat tccattccaa
     4201 aaaggaagca ataccgcaca gattctttca aaaaaccaca ataattttta attcattttt
     4261 tctatcaaaa gcacgccacg caactgatag gaggaaaaac aatggcaaca attaagcaat
     4321 atgaaaagaa agacggaacc aaagcttggc tatttcagac ctaccttgga atcagtccat
     4381 taactggtaa agaagtccgt actacacgta gaaattttaa aacaaaaaaa gaagctcaaa
     4441 tcgagcttaa tagactactt gtagactttg aagagaacgg acttaaaaag gaaacaaacg
     4501 aaactttcaa agaagtgtat gatctctggt atgagagcta taaaactact gtaaaggaag
     4561 tcactcaatt aaaaattgag attaagttta ataaatggat tcttccaaaa tatgggcagt
     4621 ttagaattaa agaaattact acaaaacatg ttcagaagat attgaatcag tgggctaaaa
     4681 caacagatca atataaagtc ctacattcta ctgcaagccg tattttcaaa tatgcaatga
     4741 ctttagggat aattactaaa aacccctgtg aacttgtaat catgcccaat agaaacgttg
     4801 aaaaaaagaa agatactgtc aaaatttatt cacgggatca actagaaagt ctcttccaat
     4861 acttggaatc aaaaagaagc acctatagag ccgactacga taaaacgcta cttcgctttc
     4921 tattttttag tggttgtcga attagcgaag ctttagcttt agattggtca gatattgact
     4981 ttgattcaaa caccgtcact ataaacaaaa ccttaagcca aacaaaatat gggtacaaaa
     5041 tttcaagtcc aaaaacgaga aatagctatg gttctctatc tttagacaat aaaaccatta
     5101 attggctaaa gaaatggcaa atcaatcaaa aaagatatat gttaaccctt ggtattactg
     5161 atccaacaat gattttttgt ggaatctaca aggaaatcat tactaaacac gctatttatt
     5221 ctcgtatgat aacaatctgc aagaattctg gtattccatt tctaggtaac catgtaacca
     5281 gacacactca tgctagtatg ctgttagagt caggagtctc catgaaggaa gttcaagaac
     5341 gccttagaca ctcatcaatc aaattaacaa tggataccta tgctcatcta actaaagaaa
     5401 ccaaagaaaa aaccgttgaa aagcttgcag agcacctaaa tttctagcgg aacgtattca
     5461 atctcgtatt caattcaaaa tacaaaaaag ctgaaccctt atctaccaag ggttcagcta
     5521 ccatttaaag cttattcttc ttcttccgcc atgaatgtat tgaatacttc ttcgatcata
     5581 tcccattctg cgtctgtttc gatttgttct aactcgcctt ctgttccatc ttcgttttcg
     5641 atgtatgcat aagcttgtaa ttctacatct tcatcttctg aaacaccagc tggatataaa
     5701 agaacatagt tacgtccaaa ttcttcttgt ccgtcaactg ttagcaagat ttcgtataat
     5761 gtttcatttc cttggtcatc caccaatgtg atgtgttcgt gtccttcgtg ttcgtgggaa
     5821 tggttatgtt ctgtcataag attacctctt ttctttttat tattaaaata attgcttacc
     5881 tgagccgtct aaataatttt gtaaaatcat cacagctgct aatttatcga tgactttttt
     5941 tcgtttcgct cttgaagtat ttgcctgctc tactagcata cgttctgcct gaacagtggt
     6001 tagtcgttca tcttgataat caaccggcaa ggagaatagt tctttgatct tctctccata
     6061 tgacatggaa gcttctgccc ttggtccgat ggaattgttc atattcttag gtaatccaac
     6121 tacaaaacgg tctacttcgt actgcgccac taattccttc aaccgatcaa aaccaaactc
     6181 ttcttctgcc tcattgattc gaatgatttc tactccttga gctgtccagc caaagggatc
     6241 gctcacagca acgcctactg tcttagaacc gacatccaag cccattactc tcataaattc
     6301 actccatgat ttgagaggta gtctttgaca attacttcca tgatttcatc gcgttcgtga
     6361 cggcgaatca aatttcgtgc atcacgataa cgaggaatgt aggccgggtc tccagaaagc
     6421 aagtagccga caatctgatt gattggattg tagccttttt cttccaaagc ccgataaaca
     6481 atcgctaatg tttcgctcac ttcttttttg cgactatcgt caaaaccaaa acgtacggtt
     6541 tcatctgtaa aacccatgat tctacacctc tttcccgcaa ggtaattcca gcttaccttc
     6601 tacaccctta ttttactcga agaaagggaa aactacaaac aaatattcca aacttctgaa
     6661 taaattaaga aacacgattt gtctgatttt tccttttttc tgtggctttt tcaaaagccc
     6721 tctttccata aagtatacgc tatatttctt tgaaagtatg tttttacaca attttttcat
     6781 aaagttcata aaaaattcga attgctgcta ctaatttttc gttttcgttg cttgccctgt
     6841 ctcataattg atggtgtagc catcttgaac attgtagata aagacattga attcaatttt
     6901 tttgtcttca atgctttctg cttccaactg cacacctctt gctaccagtt catttccctc
     6961 gaaatgagga gtgacacgat agcgtacatg atgatttgtt tccttgatat atgccgcaat
     7021 atcattttcg tgggcaagca tgtacggagc attcaacgaa cgtgtccctg tcattaagtt
     7081 tttaatgtta ttattttgac cagtcaactg gaaaccaatc aaatggctgc ggttgtataa
     7141 ccattgtccg tcacttaatt tcttttgttt ccaacctgtc ggatcaatat acaaaggttc
     7201 tctcttttca gtaggaaaca agtcttttcc caacatcgca ttggcttgtc ctactctgtt
     7261 caatttgtct atatttgaat agctttccca tgtccctttg gaaagactaa gatcctcttt
     7321 tgtaaactcc ggctgattcc cattgatttc tatttcttga tgttttccat catattctgg
     7381 cacatccgct acttgagaag cttgcggtgt ggaatcaaaa atggttgtaa tttgttggaa
     7441 gatatctgaa tctttttgtg tggtggaaat accgagtgcc cctgcaatca cagcagcgac
     7501 gatccccacg atcgccttgg ttgttttttt gctcatttct ttactccttt actaacagtt
     7561 ccctaaatct taacatatgt tcccctataa ataaatactg gaaagaaata gaaacagtaa
     7621 aaaaataaaa ccagttttta gacggcttaa gaatacaaaa aaagagacgg attcatcgtc
     7681 tctactcaat cacaaaacac tatttacaag gtatttatta tcattcaaaa aatttttctt
     7741 aatactcaaa actaaacggc ttctgtacga cctcttggta aacagtgttc aaaagcttgc
     7801 atctgcggta ataagtccaa ccaagtcaaa aacataagaa gcttttttgc ttgactattc
     7861 ttattacttg atgcagggca gctttttcac agcctctttt aagcgggtga cgggaatcga
     7921 acccgcgaca tcagcttggg aagctgggat tttaccacta aactacaccc gcgatacaaa
     7981 aacagtatac gcctctttca ctccttcctc aagttttttt attctttacg cttccgatat
     8041 gttttgtagt aaaataaaac taatcaatca attattggag aagatgctat gacgggaaaa
     8101 gaattagatc tgttgctgga cacttgttta ctcgctggga aaatcatgat ggaaagcaat
     8161 gcggagatgt atcgggtaga agatacgatg agtcgaattg ctttagcttc tggaaatttc
     8221 cgtttggtca gctatgtcac tcaaaccggt ctctttattg gtcttgatcg tacctcgacc
     8281 atccgaatgg aacaaattac caaccgttcg attaatttag aaaaagtagt aaaagtaaat
     8341 gatctttcac gtaaatatgt tgcttcagag ctaacattag aagaacttta ctgcgaactg
     8401 caagcaatcg accgagaccg ttctttcttt cctgtctggt tgcaaatcgt tagcgcggca
     8461 gtgatcagtg gaacaattat ggtgttattt ggcggaaaga tgaccgattt acctgtgact
     8521 ttagtcattg gaggattggg atacgcagct tatctatata gtttgaaatt ttttagaatc
     8581 aaatttttat cggaattttt atcttcttta ttgattggca tagccgctat ctacagcgta
     8641 cgttttggct taggaagcaa tcaggatcta atcattatcg gatgtgtgat gcctttagtt
     8701 cctggagttc aaattacgaa cgctatccgt gatttgctag ctggacatta tgtctcagga
     8761 gtttctcgcg gaacagaagc gatgatgact gctacgatga tcggttttgc gattgctttt
     8821 attttccagc ttttttatta ggaaaggaac acaagtatgg ggacgatatt tattcagttt
     8881 tcttttagtt ttttagcgac tgcggcattt gccgttatta caaatgtacc taggcgttca
     8941 ttgatttgtt gtggtctttc tggtgctttt ggctggatga tctactggat ctgtgttcaa
     9001 ataggcggat ctgctgcttt tggttcattg cttggttctt taggagtagc attcatcagc
     9061 gatcttttct ctaaacgttt gaaaatgccc gtaacgattt tcaatattcc cgggatggtt
     9121 cctctagtcc ctggtggttt agcttatcaa gcggttcgaa atctcgttac tgaatcctat
     9181 cgagaagcgg ctcattatgc ggtccaagca attatgattg ctggagcaat cgctttaggt
     9241 cttgtattat cagaagtctt taaccataac atccgtaatt tcaaagtaaa gcgtgacgca
     9301 atcatgatga aacgaaaaaa acggcttttt aaataaacga agtgtaacaa agaaaaggtt
     9361 gtgaaagaga cgttcagcct tgagcgatga caaaatatct cacaaacatt gcgacaagta
     9421 accgcaggag caataagaag taattatgag tattaaaaaa agttttgtaa aaatagcttc
     9481 tcacattttt acataaaatt gacttaatac tgagaaactg acttttgaac accctctatc
     9541 tgtaaaaaag gggctgcgac atgatttttg tcacggcccc tcttctttat tcaaattttt
     9601 tccaaaacgt tcgttgataa taaacgatcc atagagcgga aagtattaga actaaaccga
     9661 tcacaggaat attcactaac caaaatggat aaaacaaagc caacattccg tataagacga
     9721 taagaataat actgatgatc aatgaaccca tcatagttgc gactaaaccc aaacttcctg
     9781 aacctctgtt aaataattgg ttaatattgg tccagttcag cagcagcaac cgataatctc
     9841 tagcgaaata acgtagtgac aataagtagc agccgaataa agaaccaaat agcaagctag
     9901 ggataaaaaa caaaggcagc tgaaaagaca gtccgcttaa taatgcgatc ccaccagtta
     9961 aaaaactctg caatatccat ccgaatctga acttttcttt taaatattgg ttcatagaga
    10021 ttggcaaaga acggataaac agaaaatttt cttgatccaa agaaataaga ttgctgataa
    10081 aagaagtctg attaactgtt aagaaagcca atgcgatccc taccaggaaa acaacaccga
    10141 ttaacttatt atctaaatca gttaatttta tttcgcctcc tagggcaaaa gcaagaataa
    10201 aaatgattgg agttaacaag gaagaagaaa gaacttgcat aatcaaatta ggttctttta
    10261 ctaattgagc attgtaacta aataaaagct gatgcacatt ttggttcttt ttatgggttc
    10321 tttttatttt gaactcactg tttgaatcag tcagttgctc tgtaagcttt ggtactatca
    10381 gctgttttac agcaaggaaa aatcccagag ccgtgatagc tagtattccc caacccaata
    10441 aaccttgttg agaaagtggc tgactggcag catcatagaa tggcaataat atcgaaatcg
    10501 gatgccgatc tgttaactca ttttcaatag tccccgtttg atgattcatc cacatgattc
    10561 cagcaaatgc gatcagcatc gagctgccaa gcaagatact agaaactgtt tttctatgtt
    10621 ttttaaacac tgctgttcga gccaaaccaa aaacaatcaa actagccaga caaaaaagaa
    10681 taatcaaaaa tagagaaaac aaaaggattg agataatgat agtaagcaaa atccctagat
    10741 cagctcgcca tccggttaaa ataaacatga ctagaagcgg aaaaacaaaa ggaatgatgg
    10801 tcaaagcaac taccaaaatc tttgctgaaa aaacctgttc ttgtccaaaa ggcagaggca
    10861 aaaatacttg caggtcattt ccttcaaaaa agatattata aatgactgat atactttgag
    10921 ttaaacctaa cataccaaaa agtgccacgt aataagtaaa gaaacctggc aatttaacaa
    10981 agttcatagc aaacatagca aatccgtaaa tcagtaaaaa aatcagccct gaaaaaagat
    11041 tttgagacaa aagatagata gttaatcgct ttcccttttt tcctttctct cttgctttat
    11101 tcgtgagctg tggattaaca tataaaagat taactcgaat taattctaat aattgtcgct
    11161 tatacatacg gtccatctcc tgtcgcttgc cttccagcca ttttcaaata aatcttttct
    11221 agcgtttcat caggtgagcc attcaataat tcactaactg ccccctgata aatcaattca
    11281 cctttcttca aaatagccag tttatcgcat aactgttgtg cggtatccaa tacatgtgta
    11341 gaaaaaatga ctgtttttcc ttttgcggca tgagccttca tcatttcttt caaatcaaat
    11401 gctgcttgag gatcaagtcc ttgcaaaggt tcatccaata cccaaatatc tggatctggc
    11461 agcaacgtgc cgatcaaaat cgttttttga cgcattccat gtgaaaaact tgcgattgtt
    11521 tcattttgat gactatacat atcaaaaagc gctgtcaatt ctgctaaacg cttttctttt
    11581 ttttgtgtgt ctaattcata ggcagctgaa atcaagtccc aatattctcc agccgtcaat
    11641 tgtaaaaaaa tatctgggga atcaggcaca taaccgattt tttgtttgat actcaatcga
    11701 ttttccgtca atttcattcc atctactaaa atcgttcccg atgatggttc aataatacta
    11761 actaaacttt taatcgtagt tgattttccg gcaccattat gtcctaaaaa tccaaaaatt
    11821 tccccttgtc ttatcgagag agacaagtct tttaatgctt gttttttccc gtaactttta
    11881 cttacattga tgaattcaat catttctgta ctccttttgt ttgttattac tttttattgt
    11941 acccttcgtt attggtggag taaatgtcaa aaagagtgca taacaaaaaa ggatagaaac
    12001 gactaggctg ctacttgggt tgttctcttc tacaggaaaa cactcttcta acaggtaaga
    12061 tatgagtctt tgttttcttt aaaatgattc attttctatc ttttcttgca taaaattctt
    12121 tagtttatca atacttctct ttcccatctc tagttgttct ccatttgcaa atgttactat
    12181 ttcgtttact ctgctaatag atgtaatgtt ttcagtatta atgatataag cttttaaatc
    12241 tttacataaa tactcatatt cgagtgattt tttatatttt ttaattgtgc cgtttacgag
    12301 aatctcttca tctttcaata ctaggcctat tttattttta taacctggca tgctcattag
    12361 ataaaggatt tcatcgtaat ggatatagtg atcgctatta ccataacgga cagttgtcca
    12421 cctttttttc gtagcatgtt gaacaggata gttttctttt tctttcaata agtaactttg
    12481 gatgcttttt ttattttttt cataagtgaa ttcttttatc aaataaccac taggcgtaat
    12541 ctttcgatta ataacagtta ttgctttatt cggatgagac gttagataaa caattttaca
    12601 cttttcattc ttttctctaa gcatttcgcc tagttcgata ccgctataat aggcgttcaa
    12661 atcgatgtct aggaaaaaaa tatctgagga atagatttca ttatttttaa tgaattcaac
    12721 aaattgtata acgttcaatc cctgaacgat ctcaaatttc caaggactta ataaaattct
    12781 ggattcttcc aaaatttcta ctagcatttt ttcaaaaacc aaactatcat ccaaaataaa
    12841 tatacgcgtt accataaaaa gtacctccac tcgatatttt actatcaaaa tcatttcata
    12901 caacttttca acatacatgt taggaataaa ttgtaattct cttcacaatc ttttccaaca
    12961 cctttgaaac cgatatcaaa tactttcagg atgctgcttc taaaataaat atcaaacaaa
    13021 tgaatacatc atgcaaaaaa agccagacaa ccgacgtcct tgcgtgtcag ctgtctgagc
    13081 tatgtttatt tattttgttc ttttaaaaag aacgatacac atactgattt ttcggagcag
    13141 agactttctc agcgtttgta acaactacag atgggatttc acgcttgaat ggttgataat
    13201 agtctgattg gatggttcct tctattttat accattcatc atttttaacc gttactcctt
    13261 ccggcatatg cgtcaacaaa ccaaagactc ctgaatctgc cacacaatgg ataatgccga
    13321 atcggaacaa gaagatatct gtattgctat cgttcggtgt ttggtaaaca aatccttcat
    13381 agctgatttt tttgccaatg aattcgctag ggtaattata aattagctcc ataacttcta
    13441 aatagttttc atctgtgatc ttgatagttt gcttatcccc atacttttct ttcaactcat
    13501 tcatttggtc taagtagtct gatttgttga aataaatact ggtatccggc tttaagtatt
    13561 gtgtctgcat ctccggatcc cctactgatt ctttacttac tgggaaatta aatcctttgg
    13621 cttctacgat cgtagtgtcg agactaactg ttggaaacaa cgtccccacg attactggca
    13681 aagctagcag tacataagcg attccacgtt gataaggttt tgacaaccca tggtcatgat
    13741 cgtgctcgtg tgccttttct tctgcttttc cgtttttgac ccacaaatac agctgaacta
    13801 aagccaaaag agcagataaa atcattgaaa gaatagctaa gtagcgataa tggacattga
    13861 tatattgatc gagtttgcca gaaacttgca agtacatcat caattcgaaa tacccagata
    13921 aaatcaaaaa ccgcagcata ttttccctcc taaataaata atgtgaatcc aataacgcta
    13981 agggcaatca gtccgatcag cgataaaata aagcgtcctg taaaatatcg tttcatcatc
    14041 agtagatttt tgatatcgac cattgggcca aagaccaaaa aggcagctac aggtgctgtg
    14101 ccgaacagac ttaacaaaga agatcctata aacgcatctg cttctgagca aagagacatc
    14161 gtaaaggcca ataacagcat gatgataatg gctaaaatct ttgttgagcc gagcgttagc
    14221 ataattctag ttggtaaata agtctgcatc gcagctgcga ttaatccgcc aatgatcaaa
    14281 taacgacctg tatcaaaaaa ttcgtcgatt gaatgtgtca acacatgccc agtttgatgg
    14341 atcagctgat tttttggttc attcttctca ctttgttctt cttcatggac atgatgatgg
    14401 tgattttctg ctactttaga cgtgattttt tctttcaata cagcgtcttt ttgaaaatag
    14461 gcaagccaga caccaatcac aagggcaaca aaaaagctgc ctatcacccg ccagcttgcc
    14521 atctgccaag aattgccaaa tgcgataaaa gtggaaaaaa tcacgattgg attgatgatc
    14581 ggagcagtca gcataaaagc aaacgctgtg tgaactggta cgcctttttt aacaaattga
    14641 tgaacgatcg gtacgatgcc gcattcacat gaaggaaaga aaaatcctaa gatcgaacca
    14701 gtaaaaatag agaagatacg attttcagga agccatctct tcacacgttc tggggtcaaa
    14761 aatacttgta aggcgcctga gataagacag cctaataata cgaaaggtaa tgcttcaata
    14821 acgatcgata agaaaatcgt actcatctgc aaaaccgaat ttggaataaa agaaaacatg
    14881 agtttcctcc tttttatttc tagttcattc gctttgcaaa tatacgcttt tattcaaaaa
    14941 aaacaagttg ctctgtaaat ttttacacaa ctcttatttt ttaccaaagc acttcaattg
    15001 actttttctg ttattcccat taaattaagg tctcatacat atcacttcta tcataaaagg
    15061 gagaataaaa atgaaacaaa ccactaactt gactgaagtt caagacatac tgcaacagtc
    15121 atccgttgcc gtcatttatc tttcgatgcc taattgcagt gtttgtcacg ctgttcgtcc
    15181 acgactagag gaacttctca cacattatga tattccggca cttcatttgg atgcatttga
    15241 aacaccagaa gtagctagta aatttgaagt gctgactgcg ccagttgtcc tgatttttca
    15301 tcaaggaaaa gaagtctttc ggcaagcacg gttcatcgac tttaaaaaaa tccgttatct
    15361 actagatgaa ctgacagcag aagatgattc cctttcttat gaagaaatct tccgaacaaa
    15421 ataatcgttg tccaaacaga aaaaagcgat tgaacaagtg aaagttttgc cttcatttgt
    15481 tcaatcgctt tttattttta ttctgctgta ttttcggaat gcgcatttgc gtcagaatgc
    15541 aagaaataga tcaaggtttg caattcattc gtcaaatcaa cattttggat caatacatct
    15601 gacggagcat tcaatcgagc agcggtaaag ttcaagattc ctttgattcc tgcttccgtt
    15661 aactgattga ctacttcttg tgctctttca gcgggtatcg ttaaaattgc tacttcgatt
    15721 tgttggatct tgatttgttc tgacatatct tccattggat aaacggggat accgtctacg
    15781 atgcgcccaa cgatttcctc attcacatca aaagcacaac tgatacgtat gctattactt
    15841 tgatggaatt tgtattttag taaagcactt cccaagttcc ctacaccgat cagcgcaaca
    15901 tttgtcaatt gatcgtcgtt taatgtcttt gcaaaaaagt gcataagatc ttctacatca
    15961 taaccatacc cacgttttcc taattcgcca aaataagaaa aatctcgacg gatcgttgca
    16021 ctatctactt gaacggcttc acttaattca gttgaagaca cttttttctt tccagcatta
    16081 tttaaaatcc ttaaatagcg ataatacaaa ggcagtcttt tagctgtcgc ctttggaata
    16141 atatgatctt tcacgaaatg acctccacgt attgtctaat tacatctttg atttctccat
    16201 catagcagta tttataggaa aaaatcaatt ccattgctca gatattgtga aattttattc
    16261 tttattcatt tatttatcat ttctgcaaat ctcgcagtat aatggaattt atggtagact
    16321 actaattgaa aaatagtttt ttatttgagg agaattcaca aagtagaaaa gaggattcca
    16381 tgatattatt acaagccaat cagattgctc gccattttgg tgcagaagtc ttatttgaaa
    16441 acatacattt agaaatcagt acaggcgctc gtgtcgcttt agttggcaga aatggcgctg
    16501 gaaaatcgac tttattaaag atcattgctg gaattgaagc acctgatgaa ggaaagatcg
    16561 ctaaaaataa aacagcgact ctcggatacc tcgctcagga cacaggactt gattccaatg
    16621 aaacagtttg gaacgagatg ctgaaagctt ttgaacaagt tagacaaatg gaagagcgga
    16681 tgcgcgagct agagattttc attagcgaag gtacgccaga cacacctgct tatagttctt
    16741 atttaaaaga atacgaccgg ctgcaacacg atttttcaga tcgaaacggc tatggttatg
    16801 aaaatgaaat tcgttccgta ttgcatgggt ttaaattcga ttcttcattt tatgatcaga
    16861 aaatcgatac tttatcaggc ggacagcgca cacgtttagc tttagctcgc atgcttttgc
    16921 aacaccctga cattttgatt ctagacgaac caacgaacca tttagatatc gagacacttt
    16981 cctggcttga gaattatctg caaagctata ctggtgcatt attgattgtt tcccatgacc
    17041 gttatttttt agataaagta gtcaatgaag tctatgaatt aagcagaaga aaaatgcgcc
    17101 attacaaagg taactattcg aaatatctag atttgaaagc agaacagtta gcaagtgaat
    17161 ggaaggctta tgaaaagcag caaacagaaa tccataaatt agaagacttc gttgctcgga
    17221 accttgttcg cgcctctacc acaaaacgag cgcaaagccg cagaaagaca ttagaaaaaa
    17281 tggaccggct ggatcgtcct caaggggatg aaaaacaagc aaactttcta ttcagtatag
    17341 aaaagccttc tggaaatgta gtcttgcaaa tcgaagatgc cgctatcgga tatgataatg
    17401 tcaccttatc tgaaccaatc aatctagata tccgtcgtca agaagcgatt gctctagtag
    17461 gtccgaatgg tatcgggaaa tcgacgttgc tgaaatcaat cattcaccag atccctttta
    17521 tccgtggaaa agaaacgcta gggacaaatg tcaccatcgg ctattacgat caaggtcaag
    17581 ctgatctgca tacaaacaag acgatcctag cagaattgtg ggatgagcat cctaccactc
    17641 cagaaaaaga gatccgaagt gctttaggtg ctttcttatt ttctggcgaa gatgtagaaa
    17701 aaccaatccc tctgctaagt ggcggtgaaa aagcacgggt tgctttagcc aagttagcga
    17761 tgaacaaaga aaattttttg attttggatg aaccaacaaa ccatttagat attgacaaca
    17821 aagaagtatt agaaaatgca ttgatcgatt atgacggcac actgcttttt gtttcccatg
    17881 accgatattt catcaatcgg atcgcgacaa aagtcgtcga actctctgaa aatggcagca
    17941 aagtctatct aggagactat gattattatt tagagaaaaa gcaggaagaa gaagaaatcg
    18001 ccgaattgtt ggcacaagat gagccagaat ctgctcctgc tcccacgaac aaatctgatt
    18061 attaccaaag caaagaacag caaaagctta tccgtagcct tcaacgaaaa attactcagg
    18121 ttgaagaaga aatggcgcgg atcgatgaat ggatcgatca gttggaacaa gaaatggtcg
    18181 cgccagaaaa cctggaagat cacgtaaaac tcaatgagct gaaccaagat ttagaagctg
    18241 ctagacaaga tcaagaaaca aaattgacgg aatgggaaga actaagcttg gaattagaag
    18301 aatatgaaaa ttaacgaaaa aaccaaaaca gaaaccattc atgctgtttt ggtttttctt
    18361 tttttatttt atcaaacaaa aattccatat ttaccttttt caccaataag aagaccaatt
    18421 actagtcatt ctcactaaag cttctaaata atagtaatca ccccaaatac aaggtgtatt
    18481 taccccatta tttttgggct tattataaac agactgtgtt aaaaagccat cttcttcttc
    18541 cgttgtagca tatctttctt tcaattgtcg cataattttc caaacagcag actggtataa
    18601 gttcttttct gttttgtcta atagacctga ttctattaat tctaataatc cacaaaccat
    18661 gatcaccata ctagatgtat ctttttcttc tctgctagaa tcatcaaaga tcaaatccca
    18721 ataacaaatg gtatcttctg gcaaccgatt cataaaataa ttggctaata ttttgcttaa
    18781 atcaaaatat ttttgttctt tcaaataacg ataacttaat gcaaaaccgt aaatccccca
    18841 tgcttggcct ctagcccaac aggaattatc actataacct tgatgcgtac ttccatatct
    18901 tggttttcca tctttgacat ccatataaaa tgtatgataa gttgaaccat cttctcttac
    18961 taaataatct gctgcatttt gaatatgagc ctgagcagct tttttatatt tttgatttcc
    19021 agtttcttca ctcgcccaat acaataatgg aagattcatg caacaatcca tgatcattct
    19081 accttgttgt gaaggatcac taggatctcc ccaagcttgt ataactcctg ccttctccca
    19141 aaaacgctga agtaatgttt ctgcagcatc aattgaagct tccttcattt ttcgatttcc
    19201 cgttatttta taatctgcta cggttgataa agaatacaaa aaaccaagat cgtgatgatt
    19261 agtgataatt tgacagtcaa tcctgtggat aaaatctata tctgcttttt cagctgtttg
    19321 ttgaaataaa ggatcacccg tcttctcaaa taacaaccaa ttaattcctg tccaaaatcc
    19381 ttctgtccaa ccaatattgt ctcttaatgg ataacgtcct tttataggac actcgcttgg
    19441 gaattgatac tgactgttac tgatacgttt gattaacctc tccgtgtgtc tgcgacaacg
    19501 ctcgatctca ttattccatt ctttttccat taattcttta taacattcat ttatccattt
    19561 ttctggatgt attatctctt caaacatcac tttatcaagc atttccccac cagcctttag
    19621 cataatttta gtcttttttt attgttttta ttttggatag ttagcccggt tgtcccatca
    19681 aaatcaattt tgggacgatt gttccatact tctaaactat aatcccgatc ttgatgagca
    19741 accactcctg ttccgagcca atgggttcct tttccaaatg taccctctaa tccaggaata
    19801 aaagtgtttt cccatgtcat caagtttgta ttgggcaaac ccataatagc tgtggatttt
    19861 cgtttaatag tatcgtcact taaacagata atacctgaaa agccagcttc actcacccta
    19921 tagttttcct tgtcttcttc tatgattatt ccttctttct caggagaaca ttcaatagca
    19981 aaccctgtct catatagaac atattctgta tctaattcta ctttgtgtac gcgaatctgc
    20041 cagccttcaa aaggaataag ccaagttctg atttttgcct gtaaagtagg ttcccatgta
    20101 gaggcaagta cctctttctt acatacaatg tctcgtacat tatttcgaac gatgaactgt
    20161 ccataggttt tgatgctctc attcttaaaa ctggtcgtga tatatgtgtc cgctcgctga
    20221 atccctagca ttgaatccat tgccatttct gctttcaatt gattcgctcg cggtatcgaa
    20281 aaaccaaatt gactagaata agcaaatttt aaatattttt cattggtcaa tgtatggaaa
    20341 tttggcccta attgtcccgc attcaaaaaa aatgtatgtc cattatcatg ttgtatgagc
    20401 aaattggctt ctgaaagaag tttagttcct tcacgaatag gcataggcat ttctttagcc
    20461 gtccaatact catggtcttc aggtagcgcc aacaataaaa aaatcttatt cagccataat
    20521 ggagataacg tactattata gggttcagtc aatgtcaatt gagggtaggt ataaccaata
    20581 ttcaggatac ctttttcatc aaatatttct ttttgcatcc accatcttat attccgattg
    20641 actaacccct tgatttcacc taatttaaaa ggcaaaagat cggcccacaa catagctgac
    20701 caaaagcttg aaactgcaaa acgataaatc atgcttcgac caaaaggcac atttgcacca
    20761 tcttccgcaa aaaaatgaat atagtctcta gaaaaatcaa tagctcggtt cttatatatc
    20821 tggctaatgg gcttttctgg ttctagtacg ctataaatca aaccataata atgaaatgca
    20881 aaaggattat aataatcttg gcgacctctt gaactgtctc gataccagcc attttgaaga
    20941 taacaatttt ctatattatc aaatgcttct ttcagtgctg attctttaac tggataacca
    21001 aatttttgga taattgttcc gacaattatc ttaaaaaatt gccaattacc atctgcacat
    21061 tcatattcaa aaatttgcga gaaccaatga ataatctggc ttttttctcc tgtatctaat
    21121 tttttccata ataattgttc atgatgatat aacgctaata acattgcggg catttctacc
    21181 atacgctggt ctctttcatg tatttctccc caataatcgg gatgggaagg atctgttcca
    21241 gcttttattc ctgatagaat taatttcaac catcgttcat ctaattggtc aattgcgggc
    21301 cccgctcccc ataaaacccg agagaatcct tccattcctg caattttatt tccatatcct
    21361 accccttgat gtatataatc aatttgtgcg ccacttgctc gataaaacgg ctggagtggt
    21421 tgatttatct ttttaaataa gtcaaagtaa tcctgtctgg tccttaatag tggtactgga
    21481 tgtttcattg tattttctcc tctttttact accaaatagc taattccgac tgttctaatc
    21541 gaagtaagcc ttcaataaaa aaataatcag aatagatcag tggcacatta taagtcagtt
    21601 ctctatggta tgcttctgct ccgcctccaa tgatcccatc ttcatccata ttccaatttg
    21661 catagtgctt ttctgtcttt tggagtatat tgatagcagc ttcatgatac atttttgatc
    21721 gtacagagga aacatatcct gctaattcca gaagaccgca tgcagcgcat actccagcac
    21781 tggtatcaat attcgaatct tttacagcaa aatcaatgag aggaatataa ccagtttttt
    21841 cgattttctg aataaaattt actgctatct tttctgctat agctagatag tacggatttt
    21901 ttgtgtaacg ataacttaac gcgaatccat ataaagccca tgcatttcct ctgctccaac
    21961 aagaattctc attgtatcct tgcccgccaa gtaaccgaat atatttccct gattctgcat
    22021 caaattctgc gatatgcccg gttgatccat ctttcctcac tagatactta gctgttgtat
    22081 ctgcatgctg ccgagcaatt ttagagaacc tcggatcacc agtttccctc gaagcccaat
    22141 acagtaatgg taaattcatc atactatcaa taatcatcca accaggttta tggtcattcc
    22201 atgctactaa atagttccct gaaatattat agcgtccagc aagtaccgta gctgcatgta
    22261 accctagttg tcgactatgt ttgttttgag actgttgata atgtgcaata gccgatggta
    22321 agaataaaaa acctacatca tgatgaacgt ctgaaaaatg gtacagggca gattctaata
    22381 attgttcttg ttgtattgct gcctctttga acttttctcg ttttgtcttg tgaaagagct
    22441 gccataaaat accactccaa aaaccattgg tccaccagtc gattcctctg tcctttatta
    22501 cgtctgtata ttttccgttg taagaaatat atggaatttt attccccatt ctgtctaatt
    22561 ccgcatctat cttttcagaa gctacattca aaatttttct tgaccattcc ttcgtctgtt
    22621 cattcatgga tatcccgcca ttcttttaat ttttatgaat gtggagttgc tcctagaacg
    22681 aactagatgg ttaacattcg ttattttttt tacttatttg cagctccaat gtcattctcc
    22741 ttaaatttag actgcgccca cacataaatc aagaccattg ggatcactga agttgctaaa
    22801 attagcataa ttgcgccata attaattggc tcacctgtac tagaaaccgt catatttttg
    22861 ataattaagg gaaccgtata tttatcttta tctgaaagca tgactaatgg aaatagatat
    22921 tgattccaac ttcccataaa gataagtaaa ccactagtaa taatcgtagg tgataaaatc
    22981 ggtacagcga ttttcgtaaa aatcacccac tcattggcac catcgattct ggcagcctcc
    23041 atcagtgctg taggaaattg actgctatat tgtcgcatca taaacactga agttgtactg
    23101 gcaatcgaag ggatgatcac acctgcataa gtattcgata atcctaaacg actcataatg
    23161 taaaataaag gaatcaatgt ggtaaaaaaa ggcaccatcc tagatagcat aactgtattg
    23221 aacaacagtt tattccccat aaaacgatac tttgctaatg cataacccgc cattgtagaa
    23281 ctggctgtag ctgaaactgt actgattaca gcaataacaa tcgaattcca aataatcaag
    23341 ggataattat atctcgccat gatggcttgc cagtttttta atagcatatc cccaaacttg
    23401 aaattgattc cgccacttaa aatttcttga ttactttgcg ttgaagctac aatcatatat
    23461 aaatatggca tcaaaaatat taaggcacca aacacaataa tgacgtacgt aaaaattctg
    23521 ctttgtatct tttgtttagt catttttatc acctccgaat ttaaattgaa tagccgataa
    23581 tataacagat acaaacacca tcgtccaggc aatagcagag gcttttccaa aatcaaccat
    23641 ttcaaaactt gttttatata aattgactcc tacgaccaaa gctgaatttt ctggtccatt
    23701 ggaagtccca aagaatatat ttgggatatt gaaattaccc aagccattga tagtggctaa
    23761 aactgtggcc attaaaatga ttggctttag catggggatc gttaataaaa agaattgccg
    23821 aaatttcgat gctccatcta tttcagcact ttcgtatatc tctttagaaa tcccttgaag
    23881 accagctaaa aataaaatag tgttatatcc tacactaagc caaatcatcg tgatgatcat
    23941 tgatattcgt atccaaaaag gatttgttaa ccactgtaca ccgttaattc ctagcgcatt
    24001 caaaaatcca ttgaataacc cagtcggatt gaaaaaagtc atgaatacgg aagctacagc
    24061 caccgttgat gtaatggttg gaatataata gacagttcta aaaaatcctt ttccttttaa
    24121 ctgtgctgaa ttcaataaaa cagcaaagta aatagctaat agcatcgtta ttggaattga
    24181 tcctgccatg ataatcaaaa cattcgtgta agatttaata aacaatggat ctcggaagat
    24241 agctagataa ttgtcaaatc caacaaattc aaatgagtag ccaattttca taaaactaaa
    24301 agcaaacgag ataatgatgg gaatcaaaat cgtcacaata aagaaaataa caaaaggtgt
    24361 gaccaatcca taagcacttt ttttatgaga cgtatctgtt gcttttttct tgctgttctt
    24421 tattgtcttg cttggtaacg aagattgcat catcgttaag tcctcctaat attttttaat
    24481 caccagttaa ttttacgccg tacttatgag caaaattttc tgcttctttt agatatgaat
    24541 tttcaaaatc acctgtttgc caatagttat atgcagcggt agtcaaataa gacgtagcat
    24601 ctgaataagt ggctggtaaa acagtatttg atgtattttc attgatttta ttcaatagtt
    24661 catagtattt ttgtcctcct tagtagtcaa actttttctc agctgcttct gtttgatagg
    24721 ccaatgagtt agatgtagcc acgcccagct ccagtgcgtt ggctaagcat tcttcatctg
    24781 tcagcataaa tgtaagaaat tgttgtgcca cttgtggatt tttggcatct ttagggacat
    24841 aaaaacttga tcctcctgat actgcacctt ttcctggatt ttcttttgaa aaaggaatca
    24901 gtggagccat gcgccatttg tcagatgcat caggaaaatt caaagacata ttcgtagcat
    24961 accagccgcc agcaacaaac attgcagatt cttgaaatgc tttctcagca tcttgtgcac
    25021 cgtagaaatg tacaatatct gagtcgatca tttctttgat taactttgca gcatgtttcg
    25081 cttctttagt attcagacgg atatttcctt cttcatcaag caaagattga ttctgctggg
    25141 ctagcatgtt gataaacaat tcttggtccc cagattctgg caaggcaatt ccaaaactac
    25201 cagttttttc ttttaatgct tttgccacct ggatatattc ttcccaagaa ctgatttctt
    25261 caaaatctac acctatttca gcaaattttt ttggttgata ataacaaagt gctggggtaa
    25321 aatcactagc ccaaggaata atcgtttcat tactcgtttg ttgacgcaaa atattcaatt
    25381 tttgatcata aaactgatcg ccatatttgt cataaaatcc atattcatca gcactataaa
    25441 aagcatcttg aaatttttcc agcacacctg ctgctgaagt gtctgccatg gagataatat
    25501 ccggcatatc ttcgtgtgtt accaattttg gggtgatcat ttgtactgtt gctgttcccg
    25561 aagctacatt cacaattttt aagtcaatat ttgctccagt tttgcggtta tagatttttg
    25621 cagcattttc aacatagttt gtcagtttgc cgtttgcgac atatacttct aatgtagtgg
    25681 ctttttttat cattggatct gcgatgtttg ctgttacttc ttcgttagtt tttccacatg
    25741 cacttaagct gaataatagg gtaatgttta gcaaagctat caccaatttg tatctttttt
    25801 tcatgtctat cccttctttc ttttcaagtt cttatataaa acgctttcac ttatgtatat
    25861 taacggaatt agtaacacaa atatttatat aaaaaaatat tttacttgtc gttttggctt
    25921 ttcatttttg ttataatagt cattaattat aaatagaaag ctttttatat aaaaagagaa
    25981 aggaaagtgg ttcttttgtt aacaaacaac caatcctcca atcttgtcaa aaaaacacta
    26041 acctattcca tagataccca tcttcattta tctgctgctc cctattttcg actactaata
    26101 aatgaaagcg ctaatttata tgctgaattt gcacataaat ctatagaact agataatcct
    26161 tcccttttat tcattgataa acagcaagaa gtccaaatct tttctactca aaaggaaaca
    26221 caaactttta tcattatcca gttttatttt gatgagcaat tattagcaca tattacggga
    26281 caatcttttt ctatagatca agaaataaca tctatcatta cttttctgaa aaatgaacag
    26341 ttcaaaattg attatctaaa acatagtaag ccagctattt cctatgatca ggaaatcaat
    26401 tcttttttca tactatgcca acagatgagc attcatttgc tttccttatt agttacttat
    26461 tgtctgcaaa aaattcaatt gtcttcctta ccaaggcatt atcaacagga acttgcttta
    26521 ttgacaatac cttcaaatga gaaagtcatt cctaaaaaaa agcatattct ttattcagat
    26581 gaattagttg atccaatcat acgatattta gaagaacatc tttcagaaaa ttttttgctt
    26641 gaacaattag cccaaatttt ttttatcagt ccagccagct taaaaaaaca ttttagaaaa
    26701 gtgaatggaa aaagtgttat tagctatttt aaagaactaa aaatggaaaa agcaaaacgt
    26761 ctcgtgctag aaaatgaatt aacctataca gaaattgcag atatattagg atttcattcg
    26821 atccatcatt tttcttcagc ttttaaaaaa tatacagggt tatccccttc aaagtattat
    26881 ctatccttaa aatagaaaaa aatttttagg ttttatccac aaattatttc tctatatttt
    26941 ctgtctattc aaaataaaca ataattttcc ggcacaatta tttgctgttt tttaccataa
    27001 catagtacga tcaacgtaga aaaggagatg tttttatgaa agttgttgtc attggcggtg
    27061 tagctggcgg tccttcattt gccactcgtt tccgtcgatt gaatgaagcg cacgaaatca
    27121 ttatctatga acgcggagaa aatatttctt atgcaagttg tgctttgcct tattatttag
    27181 gaggtgtgat cactgaccgt gactcgctga tcgaacgtac accagaaata ttgaaaacaa
    27241 aaaacaacat cgatgtcttt actaaacacg aagtaacagc aatcgatcct tctactaagc
    27301 gattaacagt taaagaccta tccacaaatg aagaaataaa aacagattac gataagttga
    27361 tcatctcttc tggtgctaga ccagattatc cggatattcc cggagttttt gaagcagaaa
    27421 acggctttgt cctccgtagt gtgacggatg cggatcgaat caaatcgttc cttgaagaaa
    27481 aaaatccaca acatgtcgtc attcttggtg caggtgttat gggtctggaa ttagctgaga
    27541 atttcaagca tcgcggctta gacgtgactt taatcgatca attgccacaa gtcgctttcc
    27601 cttatgatcc agaaattgct aatttagttt atgacaaatt gctgaaagaa ggattagccg
    27661 ttcatttaga aacaagagtt actgagatcc gtgataaagg tcgagaaata gtattatcag
    27721 atggctccgt cctttctgct gatatgctaa tttttgctgt tggtgtttct ccgaagaatg
    27781 aagtggtgaa agcaaccggc atacaattat cagatacagg tcagatcatt gttgatgacc
    27841 agttacaaac caatctgccg gacatctatg cgattggcga tattatcgaa acgactagtg
    27901 tagtgactgg tcagccgatc caaagtatgc tttccagtgc ggccaatcgt caaggacata
    27961 tgctggcaga tattttaaat ggtacgccta tgcgctatcg cggatatatt ggtgcaggtg
    28021 tcgcaaaaat ctttgatcat acagcaagtt atgcgggaat gacagaacat gaattaaaag
    28081 catcaggcat aacaaattat aaaactgttt ttatcactcc ctttgaccat gcctatttct
    28141 atccagaagc tacaagatta aatctaaagc tgatttttga tgcagatagc ggtcgtattt
    28201 taggtggaca agcatttgga gaaaaaggtg tcgataaacg gatgggagaa ctttctgtag
    28261 caatcaccgg aaacttgaca gtctttgatt tgcctgattt ggagttgcct tactctccac
    28321 cgtattctac tacccgtgac ccgttgaata tagctggtta tgtcgcaatc aatcaaatga
    28381 cgaatatcgt agaaacgatc aaagcaagtg atatacccga aaacgatttg aaagaagcgt
    28441 tctttttaga tatacgtgaa cctaataaag caccttctgg cagtatctcg gcaacaaaaa
    28501 atattccaat gaatgagtta cgagatcgaa tcaatgaaat cccaaaagat aaaaaagttt
    28561 acattacttt cagaagagga ttgaatactt atacttctgc ccgaatcttg gcaggtttgg
    28621 gtatcaaagc gattttgatt gaagaataaa aaaacgaccg cggtgggtag ccgcggtcta
    28681 ggagttattt ttacttgagg agtaaaaatg aaaaagtata tagggttgtt ggttggtatg
    28741 tatatagaat agaagataaa tgtgaagaaa ttgtgcatta tttcttcaca tttattaggt
    28801 tatagtttaa gaaagtttaa acattgcgca atttaattat tttagctgtt tcttttcata
    28861 aaaaaacatg ttgtgactct gagacaaatc atagtccata gtgatgtctc tagagtaact
    28921 acgcctaata ttacgaggca tcatattaat ctatcagcac tagtacctaa actagcatcc
    28981 ctttcgaata ttttttctta tataataaag tttgctttca ctcctctaat ctcaccaaat
    29041 cgactggctt gcgttgtgtg atactaggaa ctgattggtt tagaacgact tttctattgt
    29101 aatcatattt ttcgccatcc tcgttgtctt ctaatccttc aatgaatgat ccctaatgtg
    29161 tgtattaaat aatttaattt ttttgttcct atttatttaa
//
LOCUS       DACECR010000027        28635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.027, whole genome shotgun sequence.
ACCESSION   DACECR010000027 DACECR010000000
VERSION     DACECR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.027"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            7..624
                     /locus_tag="IWO17_001112"
     CDS             7..624
                     /locus_tag="IWO17_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290003.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="IWO17_001113"
     CDS             1644..2813
                     /locus_tag="IWO17_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0290004.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="IWO17_001114"
     CDS             3286..3840
                     /locus_tag="IWO17_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0290005.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4486)
                     /locus_tag="IWO17_001115"
     CDS             complement(4013..4486)
                     /locus_tag="IWO17_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0290006.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4498..5034
                     /locus_tag="IWO17_001116"
     CDS             4498..5034
                     /locus_tag="IWO17_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0290007.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            5074..5445
                     /locus_tag="IWO17_001117"
     CDS             5074..5445
                     /locus_tag="IWO17_001117"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0290008.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            5729..7000
                     /locus_tag="IWO17_001118"
     CDS             5729..7000
                     /locus_tag="IWO17_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAQ0290009.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            7245..8270
                     /locus_tag="IWO17_001119"
     CDS             7245..8270
                     /locus_tag="IWO17_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0290010.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8564..9673
                     /locus_tag="IWO17_001120"
     CDS             8564..9673
                     /locus_tag="IWO17_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0290011.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            9756..10652
                     /locus_tag="IWO17_001121"
                     /pseudo
     CDS             9756..10652
                     /locus_tag="IWO17_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            10668..11492
                     /locus_tag="IWO17_001122"
     CDS             10668..11492
                     /locus_tag="IWO17_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0290012.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11524..12834
                     /locus_tag="IWO17_001123"
     CDS             11524..12834
                     /locus_tag="IWO17_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAQ0290013.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12982..14241
                     /locus_tag="IWO17_001124"
     CDS             12982..14241
                     /locus_tag="IWO17_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0290014.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14251..15123
                     /locus_tag="IWO17_001125"
     CDS             14251..15123
                     /locus_tag="IWO17_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0290015.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15135..15965
                     /locus_tag="IWO17_001126"
     CDS             15135..15965
                     /locus_tag="IWO17_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0290016.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16005..18188
                     /locus_tag="IWO17_001127"
     CDS             16005..18188
                     /locus_tag="IWO17_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0290017.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18249..19469
                     /locus_tag="IWO17_001128"
     CDS             18249..19469
                     /locus_tag="IWO17_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAQ0290018.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19466..20926
                     /gene="gtfA"
                     /locus_tag="IWO17_001129"
     CDS             19466..20926
                     /gene="gtfA"
                     /locus_tag="IWO17_001129"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAQ0290019.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21003..22109
                     /locus_tag="IWO17_001130"
                     /pseudo
     CDS             21003..22109
                     /locus_tag="IWO17_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22431..22640
                     /locus_tag="IWO17_001131"
     CDS             22431..22640
                     /locus_tag="IWO17_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0290020.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22633..22782
                     /locus_tag="IWO17_001132"
     CDS             22633..22782
                     /locus_tag="IWO17_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAQ0290021.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22817..23601
                     /locus_tag="IWO17_001133"
                     /pseudo
     CDS             22817..23601
                     /locus_tag="IWO17_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="IWO17_001134"
     CDS             complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="IWO17_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ0290022.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25304..25537
                     /locus_tag="IWO17_001135"
     CDS             25304..25537
                     /locus_tag="IWO17_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAQ0290023.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25534..25941
                     /locus_tag="IWO17_001136"
     CDS             25534..25941
                     /locus_tag="IWO17_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0290024.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(27078..27596)
                     /locus_tag="IWO17_001137"
     CDS             complement(27078..27596)
                     /locus_tag="IWO17_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ0290025.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         9843 a         4313 c         5245 g         9234 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac
     8281 atccaaaaaa gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa
     8341 aacattaagt aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg
     8401 aattaagatg aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac
     8461 attactcgca gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag
     8521 ctatgcgagt tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga
     8581 aaaacaaaat cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt
     8641 taaattagaa gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag
     8701 cgaatcctat aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga
     8761 aaatgatatt gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga
     8821 cgaactatac gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt
     8881 atttgataat gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca
     8941 agctgcaatt gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg
     9001 aacagagact ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga
     9061 caatacaaaa tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga
     9121 tattgcactt gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt
     9181 aagtcgtttt caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg
     9241 atttttgaaa aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa
     9301 agttaatgca acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc
     9361 agaagaagtg aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc
     9421 ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc
     9481 agatacaatt gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata
     9541 caataaagat ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc
     9601 tggtgaagta actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt
     9661 gagcggaaat taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg
     9721 tatattaatg gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat
     9781 atcgtaaaga tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa
     9841 ttttattaaa tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag
     9901 ggttcggtga tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg
     9961 aagtttttca gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg
    10021 ttgtattatc cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt
    10081 atcggacaat ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc
    10141 gctggatgtt caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg
    10201 atggaccagc atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag
    10261 tttggagtgc tattggttat aacatggtgt tattactagc cggcttataa gaaattccta
    10321 aagattatta tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca
    10381 ttacgttacc attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg
    10441 ctttccaagt atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta
    10501 aatcacaatc attagcctat ttattctata aacattcatt tattttaaat gacaagggat
    10561 atggttcggc aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa
    10621 tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat
    10681 ttttttcaaa tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac
    10741 cttttatttg gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac
    10801 ctacaatttt ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac
    10861 cattcatggc tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg
    10921 ttttattttc agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata
    10981 tccttttcag cttagtactt attcaaatga tgattcccgg ttccattttt attactccac
    11041 agtatttatt agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag
    11101 gggtagttag tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg
    11161 aattggagga agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta
    11221 tgttgccgct tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat
    11281 ttaaagattt aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc
    11341 cgggattagc aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt
    11401 cgttattagc aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg
    11461 aaggaattgc tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag
    11521 tacatgacaa aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta
    11581 ggcgatgcga tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat
    11641 gaaaaacgac taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa
    11701 aatcgtgctg atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt
    11761 gttatcaatg ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt
    11821 cctaaaaaat atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt
    11881 agaaccctac ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt
    11941 ccaaatgctt ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta
    12001 aaagcgacga gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa
    12061 ttgtttgagc atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt
    12121 gcaggcatta atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat
    12181 ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt
    12241 gaattgttga aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac
    12301 catccatact ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg
    12361 gatgaatact tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac
    12421 attgtaaata atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg
    12481 gattctatta cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta
    12541 atcacaaact taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc
    12601 gtgcaaccaa cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat
    12661 atcaatgttc aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat
    12721 caagcagcgt atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat
    12781 ttagtggatg atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa
    12841 gctgaaagag taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat
    12901 attgacaaaa cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa
    12961 ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca
    13021 ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag
    13081 gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct
    13141 aaagattttg aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct
    13201 ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat
    13261 cctcaaagta tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa
    13321 gaaccgtatt tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt
    13381 gtatatagca taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt
    13441 gaggaaatgg ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag
    13501 attaaagatc aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat
    13561 ggttatcacc aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt
    13621 tggcgtttct cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt
    13681 aatcgattag atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt
    13741 tataacgata cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca
    13801 tgggcaatgc cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt
    13861 ccagcagatg aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt
    13921 tctgcaactt ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct
    13981 gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc
    14041 gagaatactc aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat
    14101 ttagtttggt tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat
    14161 tatttacaca atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca
    14221 atgaaagctg acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac
    14281 tgggcaatca tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca
    14341 atggttcaag gtgcttttta cagtttaact aattggactg gtctgactta taattttgat
    14401 tttgtgggtt tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt
    14461 ggcttcactt taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg
    14521 attgcgcggg cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt
    14581 tttccagcgg tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat
    14641 ggtttaccaa aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga
    14701 acaacatggg gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg
    14761 ccagtcatca ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct
    14821 agaatggatg gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta
    14881 ccaagtattt caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa
    14941 atttttgctt taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt
    15001 tataattatg cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta
    15061 tttatcttaa ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc
    15121 taggaggttt cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat
    15181 tattaggggg cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa
    15241 caacacccga tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt
    15301 tccgaagatt gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa
    15361 tcattgcagt agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa
    15421 aaatgactcg tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg
    15481 ttcctttcca agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa
    15541 atatgtgggg attaatctta ctctatctca cttatgccgt accacagaca ctatttttat
    15601 atgttggttt tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg
    15661 gggctagtca ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg
    15721 cgacgactat tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa
    15781 ttttaaatcg tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac
    15841 aatattttaa cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta
    15901 cactagttta tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga
    15961 aataaccaat aaattattct cattataaag gagttcataa acttatgatt atatttgaca
    16021 atcacacaca aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag
    16081 aaaataatta tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt
    16141 atcgctaccc ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt
    16201 tattatcttt agacacatta ccattagaat ttccaggata tggacatggt gattttagag
    16261 aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata
    16321 gctacgaaat tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag
    16381 atgatgatga agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt
    16441 tacagttgtt ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag
    16501 taaataatag tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc
    16561 caaatcgtcc actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga
    16621 ctagagaaaa aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc
    16681 atcaacaaaa tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg
    16741 catatggatt taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat
    16801 ttgaccaaac acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa
    16861 ctggcgactc atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata
    16921 cgttatcaca gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt
    16981 taaaagaacg tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag
    17041 aaaaaatcat gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag
    17101 atgatggttg gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa
    17161 cagaaggcaa acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga
    17221 tgaaatttgg gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag
    17281 cacatccaga ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat
    17341 atgttcttga tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga
    17401 tttttgatcg tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag
    17461 tctattctgt cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag
    17521 ggttatatga atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt
    17581 gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga
    17641 cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt
    17701 atccaatttc aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag
    17761 aaacgagttt aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag
    17821 atctaacgca attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata
    17881 aaaagcatcg ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag
    17941 gcaatgatac tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt
    18001 tccgagtatt agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag
    18061 aagaagcaac ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg
    18121 gtttttatat tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac
    18181 agatatagta gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg
    18241 aagaaaatat gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa
    18301 atgaagaagt acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag
    18361 gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg
    18421 aatctgatgg agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca
    18481 ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg
    18541 tagcagaagc cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt
    18601 attcgatggt ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt
    18661 ttaaagatag ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag
    18721 ttacgcaaga taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa
    18781 ttaataaata tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc
    18841 tttttatgtt actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca
    18901 acatagaaat ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac
    18961 gggcattaga atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc
    19021 gggataattc tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt
    19081 ctgataatgt atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt
    19141 taaccaatca acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata
    19201 gtgtatacaa agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac
    19261 atattttgct ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta
    19321 cagcaacaga acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt
    19381 atgaagatgt tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg
    19441 tattagctaa ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta
    19501 tgcagatagt ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca
    19561 aggtgttatt ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt
    19621 tgcgccgagt gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc
    19681 attaggtgaa gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc
    19741 aaaatttttc caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat
    19801 ccgtattcat gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta
    19861 caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga
    19921 ggtttggaat acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa
    19981 agaatttatt cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt
    20041 agatgcgttt gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga
    20101 tatttgggaa cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt
    20161 accagaaatc catgagcatt attccattca aatgaaaatt gccaatcatg attattatat
    20221 ctatgacttc gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg
    20281 tttagccaat tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga
    20341 tggaattggt gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc
    20401 tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa
    20461 taatttagat atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg
    20521 tagctatctg ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta
    20581 cgttgggtta ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg
    20641 taatattaat cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt
    20701 gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga
    20761 tggaacgatt gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa
    20821 aaacggtagt gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga
    20881 gtctaaagat ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa
    20941 caaaaaatcc tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat
    21001 acatggataa gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag
    21061 gaaaactttc tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac
    21121 tgtcttttga agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt
    21181 tgcgtgattt gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat
    21241 cgattagtca tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag
    21301 aaatttttca acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc
    21361 atctgtatct gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga
    21421 ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata
    21481 ctacgatgta tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac
    21541 taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact
    21601 ttgaaaagat ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa
    21661 acaccaaagt tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag
    21721 acgagctggt tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg
    21781 ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa
    21841 aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca
    21901 aacaacacat gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat
    21961 tgattttaac gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg
    22021 aaaaatcagt gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat
    22081 gttgtctaga aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt
    22141 tttcagttga ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag
    22201 ttttttttga cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca
    22261 acactagtga tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc
    22321 aagtatacaa agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact
    22381 atttttagta gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta
    22441 taacaaaaaa tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac
    22501 gacaggcaaa agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag
    22561 gactagtacc tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg
    22621 attttcagaa tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct
    22681 agtttgggat tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa
    22741 cagttaaaga agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga
    22801 aacttctcaa agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta
    22861 tcaagggaaa gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt
    22921 tcttatttct ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa
    22981 aataatttat tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag
    23041 atgtcattag atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt
    23101 ggaaatgtga agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa
    23161 gttatttcaa ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg
    23221 ttacttttta tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt
    23281 gattttgaaa ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc
    23341 tatgagtttg tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat
    23401 acaattacct ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt
    23461 gtactttctt ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga
    23521 catgcaacga tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct
    23581 tctttgctta tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct
    23641 gatgtacaaa cgactttagg aacttatggt catttgtatc taaattcatt gagagagata
    23701 acaaatgatc ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt
    23761 tacgataata aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag
    23821 aaaaactata atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt
    23881 atactcataa agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag
    23941 ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct
    24001 gttggtagag tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa
    24061 agccttttga atattttgct accatccgtt cgattgcaat accggatata cgcttggctt
    24121 ctccttggta aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt
    24181 tccgaatagc tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat
    24241 ctcttttatt tccttttcta gttactacca cattattctt atctaaagat aggtcatcta
    24301 aattaatatt tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg
    24361 ccaggtctct ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt
    24421 tatattcatt ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt
    24481 tctcaatggc attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat
    24541 ccataggatt attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat
    24601 atttaaatag aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta
    24661 agttattatt atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc
    24721 gacgtttcaa atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg
    24781 tgatagaagt atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat
    24841 tttttagata ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa
    24901 aatcttttat ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg
    24961 tatttttcat gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga
    25021 ctataccaat tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc
    25081 aaaaaaagca tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata
    25141 gggaaggaaa tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga
    25201 aagaacaatc tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca
    25261 agcgtatata ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag
    25321 aaaatttagg gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga
    25381 aaatgggtta aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat
    25441 tacaaaaaaa caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga
    25501 atatgatact acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa
    25561 gatattataa aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt
    25621 gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt
    25681 gaagaggttt atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag
    25741 aaacatcctt ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg
    25801 aaaatcaatg gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat
    25861 attgctacgt atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag
    25921 acacatacgc agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct
    25981 atagcgaaat aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa
    26041 acgtaaattt tctatatagt tggattaaga taacgactat ttattttatc atatttttac
    26101 tataccctat acttttactt ggtatattta tgaataatcg caaatttacc cctatactac
    26161 tagtttgtaa gttagattaa taaaaatact aaagttattt gttcttttat taaatacacc
    26221 tacaacttat aaattgatgg tatttagata acacgaaaga ataaaaataa cagctgatag
    26281 taaagtttgt tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc
    26341 tgaaaactta tgaaatgaaa gaccttttac atcttgcaga aatcggtact gggattaaac
    26401 taagcaataa aaactaatgt tcccctcatt ctttgaactt ctactatttg atttatttat
    26461 tctgataaat gacgatttca ccttcaggat cttcaaatcc cgtatagaat ttatttaaga
    26521 tctcttcagt atcagtaata ggataggcga taggccacat accctttgat ggttagaata
    26581 aactgctcaa aatctgtatc gtatgttggc tttagcatat taacaaaggc tatctccaaa
    26641 ttataaacca taagcgttga atcgttctta acaatttcaa ttcttcctaa cccaaagtca
    26701 gaagatataa ttaccggact tgccttaccc ttcatgtggg ttgttgagat tccatattta
    26761 aagggaattg tcggtactat cggcggtctg aaaaacatcc caaaaataaa aaaatctttc
    26821 ccctgctagt ctcctaagtc cctttgtagc ggatatggca ccataatctc attttatcga
    26881 agtgagtcat ggtttatctg ttggccaacc aaaattgata caatctaacg atgcaccctc
    26941 tacggcattc taggggagtg cacttgcatt cacccaataa aaaccggcag ttagcaaaga
    27001 acttatgtag tcgtatgttc ttactgttaa catacaagag gtctcctttc cacaattgct
    27061 tagcagtaaa cgggaattta caaagctaat ctcttccatt ttcttatctt tgttctaaaa
    27121 gtcccaaatg gagcaactgt atttacatga ataaatttat atacttccca tacagccgtt
    27181 ttggttgcct cgtctgccca ttttcttttg tgtgcagtga atagatcttc atcactcatt
    27241 gaatcaatca tcatgttaat atcatcaata ttacgattga gaatttcctc taattctttc
    27301 aatggtagat gtgcataggt atttgtaaac cacttatata actctcctaa ctgattccat
    27361 ttaaattgat cagaaggcgt tttaacagat aatccatttc tttcatcatt ttcccatttt
    27421 agaattaaat tcgtccaacc aacttgatat gctaaatttt cagcaggtgt tctatccact
    27481 tcctcaactc ttttatcctt caattcttca ggaatagttt caaattctaa aatatattta
    27541 gtataagtcc tatttatttc tttttttaat tcttctttat ttttatatgt tctcattata
    27601 ttacctccat aaattcctaa ttgtctcctt taattcatct ataatttcat ccacgatatt
    27661 ttaatccgtt ctacaatgca tatctgatat gcattgactg tctaactcat atgtgaatga
    27721 tttataaaat atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa ggagctaaaa
    27781 aatacgaata tatttctttt gtagtaaaat cggactggtt tgcatatcat tttcttcgta
    27841 aatatcaaaa tcatgttaac tactatcaat tactaataaa aactaccact ttaagcgtta
    27901 agaactgtta agtttagtta agtgaggata gttcacaaaa tccattgtta aacttcacga
    27961 aagtggtaaa actcaaaatg ctttatatag gattacagca tcgctctttc ttcgattgcg
    28021 cgttgggtaa aaaatattat gaagtaaaaa ttgacgatga cacgttcctt acggctcatc
    28081 agatgaaaca gctacaaaaa cgaaatgatc aactcgaaga aaatttaatt ctaaaaaagc
    28141 gattgccata ttcacgcctc gctcgacgaa ctattaaagg tcaagattta cctgaagatc
    28201 atccttttta ggaaattgaa gctttttatg ttgcacaatg catttataat actacaatga
    28261 tgttttcacc agatattctt ctagggtgcg gtgtaatgaa acagatacac ttgcaaaaga
    28321 aaattcaccg cgaatttact aaaattatga ataattatgt taatcttccg aaattttttt
    28381 ccaaaattaa gggatcaagc tggtgttata gattgtttaa ggttggcaaa aaaataactt
    28441 ctttttaaac aattatagaa aaccgatact ctgttaagtt ggttgacacg gtgtcgattt
    28501 ttatgacttt cttattctga aacttcacta acggacagta cctaaaaagt ctatagtatg
    28561 gacgtttacg agtactattt tatcaaaaat gctgctcatt tcaacttata tgttttagtg
    28621 aactaccccc actta
//
LOCUS       DACECR010000028        27997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.028, whole genome shotgun sequence.
ACCESSION   DACECR010000028 DACECR010000000
VERSION     DACECR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.028"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(5..586)
                     /locus_tag="IWO17_001138"
     CDS             complement(5..586)
                     /locus_tag="IWO17_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0290026.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="IWO17_001139"
     CDS             complement(769..1395)
                     /locus_tag="IWO17_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0290027.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="IWO17_001140"
     CDS             complement(1417..2748)
                     /locus_tag="IWO17_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ0290028.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="IWO17_001141"
     CDS             complement(2903..3304)
                     /locus_tag="IWO17_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0290029.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="IWO17_001142"
     CDS             complement(3390..3596)
                     /locus_tag="IWO17_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290030.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="IWO17_001143"
     CDS             complement(3691..5193)
                     /locus_tag="IWO17_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ0290031.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IWO17_001144"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IWO17_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0290032.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="IWO17_001145"
     CDS             complement(5764..6381)
                     /locus_tag="IWO17_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290033.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="IWO17_001146"
     CDS             6602..7975
                     /locus_tag="IWO17_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0290034.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="IWO17_001147"
     CDS             8050..9162
                     /locus_tag="IWO17_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ0290035.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="IWO17_001148"
     CDS             complement(9234..10871)
                     /locus_tag="IWO17_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ0290036.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="IWO17_001149"
     CDS             complement(11039..11647)
                     /locus_tag="IWO17_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0290037.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IWO17_001150"
     CDS             complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IWO17_001150"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ0290038.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13201..13953)
                     /locus_tag="IWO17_001151"
     CDS             complement(13201..13953)
                     /locus_tag="IWO17_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290039.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14094..15044)
                     /locus_tag="IWO17_001152"
     CDS             complement(14094..15044)
                     /locus_tag="IWO17_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0290040.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15045..15884)
                     /locus_tag="IWO17_001153"
     CDS             complement(15045..15884)
                     /locus_tag="IWO17_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0290041.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15881..16627)
                     /locus_tag="IWO17_001154"
     CDS             complement(15881..16627)
                     /locus_tag="IWO17_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0290042.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16865..18235
                     /locus_tag="IWO17_001155"
     CDS             16865..18235
                     /locus_tag="IWO17_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0290043.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18290..19186)
                     /locus_tag="IWO17_001156"
     CDS             complement(18290..19186)
                     /locus_tag="IWO17_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0290044.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19467..20144)
                     /locus_tag="IWO17_001157"
     CDS             complement(19467..20144)
                     /locus_tag="IWO17_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0290045.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20235..21140)
                     /locus_tag="IWO17_001158"
     CDS             complement(20235..21140)
                     /locus_tag="IWO17_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0290046.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21315..22769
                     /locus_tag="IWO17_001159"
     CDS             21315..22769
                     /locus_tag="IWO17_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0290047.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            22785..23816
                     /locus_tag="IWO17_001160"
     CDS             22785..23816
                     /locus_tag="IWO17_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0290048.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23823..25010
                     /locus_tag="IWO17_001161"
     CDS             23823..25010
                     /locus_tag="IWO17_001161"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ0290049.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25166..26548
                     /locus_tag="IWO17_001162"
     CDS             25166..26548
                     /locus_tag="IWO17_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0290050.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26675..26905
                     /locus_tag="IWO17_001163"
     CDS             26675..26905
                     /locus_tag="IWO17_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290051.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27105..27773)
                     /locus_tag="IWO17_001164"
     CDS             complement(27105..27773)
                     /locus_tag="IWO17_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ0290052.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         7792 a         5726 c         5146 g         9333 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgtttc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    11881 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    11941 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    12001 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    12061 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    12121 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    12181 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    12241 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    12301 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    12361 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    12421 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    12481 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    12541 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    12601 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    12661 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    12721 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    12781 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    12841 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    12901 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    12961 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    13021 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    13081 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc
    13141 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    13201 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    13261 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    13321 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    13381 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    13441 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    13501 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    13561 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    13621 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    13681 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    13741 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    13801 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    13861 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    13921 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    13981 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    14041 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    14101 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    14161 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    14221 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    14281 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    14341 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    14401 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    14461 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    14521 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    14581 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    14641 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    14701 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    14761 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    14821 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    14881 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    14941 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    15001 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    15061 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    15121 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    15181 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    15241 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    15301 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    15361 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    15421 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    15481 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    15541 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    15601 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    15661 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    15721 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    15781 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    15841 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    15901 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    15961 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    16021 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    16081 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    16141 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    16201 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    16261 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    16321 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    16381 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    16441 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    16501 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    16561 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    16621 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    16681 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    16741 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    16801 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    16861 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    16921 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    16981 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    17041 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    17101 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    17161 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    17221 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    17281 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    17341 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    17401 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    17461 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    17521 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    17581 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    17641 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    17701 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    17761 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    17821 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    17881 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    17941 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    18001 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    18061 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    18121 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    18181 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    18241 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    18301 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    18361 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    18421 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    18481 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    18541 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    18601 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    18661 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    18721 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    18781 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    18841 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    18901 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    18961 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    19021 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    19081 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc
    19141 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    19201 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    19261 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    19321 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    19381 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    19441 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    19501 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    19561 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    19621 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    19681 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    19741 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    19801 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    19861 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    19921 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    19981 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    20041 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    20101 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    20161 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    20221 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    20281 aaggtatcca aagcaatatt cttaaaattt tcgtttttca tacgtttacg atagttcgta
    20341 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    20401 acttgatagg caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga
    20461 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    20521 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    20581 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    20641 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    20701 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    20761 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    20821 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    20881 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    20941 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    21001 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    21061 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    21121 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    21181 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    21241 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    21301 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    21361 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    21421 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    21481 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg
    21541 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    21601 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    21661 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    21721 aggatatcgc tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa
    21781 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    21841 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    21901 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    21961 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    22021 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    22081 atatcgctta tgcgattgcc aatgtagcaa caactgccgt attattctac ttcttcaaat
    22141 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    22201 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    22261 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    22321 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    22381 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    22441 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    22501 acgggatcgt agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg
    22561 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    22621 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    22681 cacaaatcgt tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    22741 ttcacgatgc agtagttcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    22801 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    22861 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    22921 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    22981 gtcgcaccgc agggagaatc gaaaatggcc gcttttctct caatggaaaa acttaccaat
    23041 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    23101 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    23161 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    23221 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    23281 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    23341 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    23401 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    23461 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    23521 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    23581 gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    23641 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    23701 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    23761 ccggatcagt atttgaaaca gtaacagaat tcaaaatcaa aacaagaaag gaataactca
    23821 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    23881 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    23941 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    24001 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    24061 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    24121 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag
    24181 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    24241 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    24301 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    24361 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    24421 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    24481 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    24541 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    24601 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    24661 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    24721 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    24781 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    24841 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    24901 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    24961 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    25021 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    25081 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc
    25141 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    25201 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    25261 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    25321 cgattcgaca gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa
    25381 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    25441 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    25501 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    25561 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    25621 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    25681 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    25741 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    25801 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    25861 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    25921 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    25981 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    26041 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    26101 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    26161 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    26221 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    26281 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    26341 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    26401 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    26461 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    26521 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    26581 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaatgaaga
    26641 agaagcacgc aaacagcctc ttcctaaaca acaaatgaca ttgcaagaaa tcgatagtat
    26701 actgacagaa gcctttttat ctcgtaaagc agtaatcatt cagctcaatt taaaagatga
    26761 atttggaaga ctcttggatg aggtcgaagg gcacttcatt ggtgaagcct atgaagatta
    26821 ctttatgatt gaccatcagc cgatattgtg ggaagacgta cggtatattg agttaaagga
    26881 aacaaaaaaa tggtttgtga catgagaggt tgtgacagaa gtgatcaact tcaaaaaata
    26941 agaaaggatt tttcgaaaat tgcttcttaa atttttgtgg aattttggct tatttttcga
    27001 agaagcaact tctggaacaa cgtatattag atatcagagg ttgtgaaaaa gtcgcttagt
    27061 tttgagtaac aagccaaggc gctgatttca caacctctgt ctatctatcc atctattcgg
    27121 taagattcat gcatgacgtc ttctttattt agtatgtaga gccctttttc ttctgaccat
    27181 tcaacataaa tcagctgttt tacatctgca tagccttctt tcttcaagtt ctccataagc
    27241 catgcttcag tcaattgatt ggactgaagc actttatgct gaatttgccc ttcatctaca
    27301 agcaggatcg ataaagcttt gtcttcatct gattttttca atacactcat ttgaccggca
    27361 ttctctagga taacatgttt ggcattttca agaaaaaagc attcttgctg tcttaagatt
    27421 gctcgcaact gctccatctc gatatggtta tttgtcaact ctgtcatgtt gatgacgccg
    27481 tctttgatta gaacagatgg ctcgcctttg acccaacgat tgaccttttc atttttttgg
    27541 acgatacgtt cgataccata aatcattact gcccaaagcg caatggcaaa taggacatgc
    27601 cctacatgga cattgtcatc ataggttgat tcttctaaga tcccacctaa aacaagcgta
    27661 tagactaaat caaacggtgt gatttctgac atggatttct tcccaagcaa acgtaccact
    27721 aataataaac cgatcaaccc gaacaccagt ttcattgtaa ttgaaaaaat cattttttct
    27781 ccccctaact atctgttaat taaattatat atgctttctc acgaacacgc atggaatata
    27841 caaggaagaa atgtatcctt ttactaaaaa aaggactgtg acactgtagt gacccccaaa
    27901 agttagactt ttttggagtg gagaaatcca ctcctttttt ttatgctcta aaatgatatt
    27961 tttacacaac cgttacggtt gttttacgaa attggac
//
LOCUS       DACECR010000029        27180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.029, whole genome shotgun sequence.
ACCESSION   DACECR010000029 DACECR010000000
VERSION     DACECR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.029"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..860)
                     /locus_tag="IWO17_001165"
     CDS             complement(<1..860)
                     /locus_tag="IWO17_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0290053.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLL"
     gene            1133..3604
                     /locus_tag="IWO17_001166"
     CDS             1133..3604
                     /locus_tag="IWO17_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0290054.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HANQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3707..5443)
                     /locus_tag="IWO17_001167"
     CDS             complement(3707..5443)
                     /locus_tag="IWO17_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ0290055.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5440..6144)
                     /locus_tag="IWO17_001168"
     CDS             complement(5440..6144)
                     /locus_tag="IWO17_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0290056.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6275..6778
                     /locus_tag="IWO17_001169"
     CDS             6275..6778
                     /locus_tag="IWO17_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ0290057.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6738..7076
                     /locus_tag="IWO17_001170"
     CDS             6738..7076
                     /locus_tag="IWO17_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ0290058.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7097..8515
                     /gene="ffh"
                     /locus_tag="IWO17_001171"
     CDS             7097..8515
                     /gene="ffh"
                     /locus_tag="IWO17_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ0290059.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8627..9205
                     /locus_tag="IWO17_001172"
     CDS             8627..9205
                     /locus_tag="IWO17_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ0290060.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9209..9730
                     /locus_tag="IWO17_001173"
     CDS             9209..9730
                     /locus_tag="IWO17_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0290061.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9809..10363)
                     /locus_tag="IWO17_001174"
     CDS             complement(9809..10363)
                     /locus_tag="IWO17_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0290062.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10616..10891
                     /gene="rpsP"
                     /locus_tag="IWO17_001175"
     CDS             10616..10891
                     /gene="rpsP"
                     /locus_tag="IWO17_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ0290063.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10903..11154
                     /locus_tag="IWO17_001176"
     CDS             10903..11154
                     /locus_tag="IWO17_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0290064.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11279..12070
                     /locus_tag="IWO17_001177"
     CDS             11279..12070
                     /locus_tag="IWO17_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290065.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12240..12761
                     /gene="rimM"
                     /locus_tag="IWO17_001178"
     CDS             12240..12761
                     /gene="rimM"
                     /locus_tag="IWO17_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ0290066.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12751..13512
                     /gene="trmD"
                     /locus_tag="IWO17_001179"
     CDS             12751..13512
                     /gene="trmD"
                     /locus_tag="IWO17_001179"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ0290067.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13648..13995
                     /gene="rplS"
                     /locus_tag="IWO17_001180"
     CDS             13648..13995
                     /gene="rplS"
                     /locus_tag="IWO17_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ0290068.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(14127..15263)
                     /locus_tag="IWO17_001181"
     CDS             complement(14127..15263)
                     /locus_tag="IWO17_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0290069.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(15379..15945)
                     /locus_tag="IWO17_001182"
     CDS             complement(15379..15945)
                     /locus_tag="IWO17_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290070.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(16081..16734)
                     /locus_tag="IWO17_001183"
     CDS             complement(16081..16734)
                     /locus_tag="IWO17_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290071.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(16784..17212)
                     /locus_tag="IWO17_001184"
     CDS             complement(16784..17212)
                     /locus_tag="IWO17_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0290072.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(17217..17591)
                     /locus_tag="IWO17_001185"
     CDS             complement(17217..17591)
                     /locus_tag="IWO17_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0290073.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            17895..18080
                     /locus_tag="IWO17_001186"
     CDS             17895..18080
                     /locus_tag="IWO17_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0290074.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            18093..18341
                     /locus_tag="IWO17_001187"
     CDS             18093..18341
                     /locus_tag="IWO17_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290075.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            complement(18644..19102)
                     /locus_tag="IWO17_001188"
     CDS             complement(18644..19102)
                     /locus_tag="IWO17_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290076.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            19270..19587
                     /locus_tag="IWO17_001189"
     CDS             19270..19587
                     /locus_tag="IWO17_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290077.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            19520..19999
                     /locus_tag="IWO17_001190"
     CDS             19520..19999
                     /locus_tag="IWO17_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HAQ0290078.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            20449..20880
                     /locus_tag="IWO17_001191"
     CDS             20449..20880
                     /locus_tag="IWO17_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290079.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            20877..21203
                     /locus_tag="IWO17_001192"
     CDS             20877..21203
                     /locus_tag="IWO17_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0290080.1"
                     /translation="MKELTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSL
                     IAIDSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEI
                     IAHEPE"
     gene            21322..22008
                     /locus_tag="IWO17_001193"
     CDS             21322..22008
                     /locus_tag="IWO17_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0290081.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            21983..23332
                     /locus_tag="IWO17_001194"
     CDS             21983..23332
                     /locus_tag="IWO17_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0290082.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            23323..23796
                     /locus_tag="IWO17_001195"
     CDS             23323..23796
                     /locus_tag="IWO17_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0290083.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            23798..24298
                     /locus_tag="IWO17_001196"
     CDS             23798..24298
                     /locus_tag="IWO17_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HAQ0290084.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            24313..26625
                     /locus_tag="IWO17_001197"
     CDS             24313..26625
                     /locus_tag="IWO17_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0290085.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            26872..>27180
                     /locus_tag="IWO17_001198"
     CDS             26872..>27180
                     /locus_tag="IWO17_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HAQ0290086.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     E"
BASE COUNT         8978 a         4810 c         5551 g         7841 t
ORIGIN      
        1 ttgagtaaga gtttccaata tcgcttgagc cgacggtact tcttctgatt ttcagagtgt
       61 gaggttcgaa aacgattcat gatctggaca cgtaattgat tgaagctgcg attgatgtgt
      121 tggacaatat gaaagcggtc agttacgatc tccgcatttg gaaagactgt tttaagcaac
      181 tgacaatagc tggcattcat gtccatcact aggtacttga cctttaaacg ggcctttcta
      241 ggaaagcgca ggaagtattg agttagtttg taaagtctgc gatcttcaag gatcgtaaat
      301 agctgattgg ttgtcccatc aacacaaata aaactcatgg aaccagaaac agaattcatt
      361 gatttgaact catccataca caacaccgtc ggaaggaagt tcatacgtgg ctggtaatgc
      421 ttcgttgctt cacgcaagat ccgtgtcacc gtcccatccg agacgaagta cttcttagcg
      481 attagtttac gtgattgatt ttcgtacagt tccatgagaa tcgcctgctt cagttccttt
      541 gttagttgat gatgttcatc aactaagtcc gttttcgcag aaaaggtcct tccacaatca
      601 tggcagagaa aacgggtacg ttttaattct aaaatagtta acttttcttt gactttcaac
      661 atctgagtgc gtgtcgtata ggaaccgttg cggataatac gaaccgatcg acagtgttca
      721 caataggaag aagagtcatc caattttcct ttaataaact gcgcttgaac gcctttatat
      781 tctttttcaa tcagccattc ttcatcaaaa aataaatgtt tatctgttaa tccgagtaat
      841 tttctagtat gattatccat tgagagactc ctttggtagg atgacgtttt ggtcgactaa
      901 attttaccaa aagtgagtct ctttttgagt tgaaacgaag aaaaagtgtt agtagaaaat
      961 cgccttcgat tttccaccaa cacaaaatat tatagaaccc tttttttttg ctatatgaca
     1021 ggaatgaaag gtgtttgcaa gcttaatgtg aggaacactt tccatatctg ttctatactg
     1081 gataggagaa gtttggaaat atccagaaaa cgaaatcaat cgaggtagcc acatgaagaa
     1141 taaatggata aatcgaagta aaaaaaagaa agtaacacaa agatcatcag ataaagtcat
     1201 ccgtcttttt ccatcgtata aagaaggatt aacggcagat caagtcaagg aacgaataga
     1261 aaaaggtgca gcaaataatt cagtcgatcc cacattcaaa acaaatcagc aaatcgtatt
     1321 ggaaaacata tttacgtatt tcaatttgat ctttcttatc ttagccattt tgctttgttt
     1381 agtagaatcc tataagaact tgactttttt accagttatc atagccaata caggaatcgc
     1441 catttttcag gaaattcgat ccaaaaagat cttggacgaa ctaaatgttt taaatacagc
     1501 aaaagtcaat gtcatacgag acgggataga acaaacaata gatatagaag accttgtaat
     1561 agatgacatt gttattttag agactggata tcaaattcca gcagatgcag aggtcgtgga
     1621 agggaaactg caagttaatg aggctttatt gacaggcgaa gcagatgaaa taaccaaaga
     1681 aataggtgat ccattaatgt ccggcagttt cattgtttcc ggaaaagctt acgcccgatt
     1741 agacaaagtt ggagcagatt cgtatatttc ccagctgaca gtcaaagcca agacaatggg
     1801 agaaggagaa cagtcggaga tgattgcttc tcttaatcaa ttgatcaaat ggattgggat
     1861 catcattatt cctatcggta tcctgctttt ttctcaaagt tactttttta atggaaacac
     1921 gatcaaagaa agtgtcgttg caatggaagc agcattgatc ggtatgatac ctgaaggact
     1981 gtacttgctg acgacgattg cattagcatt aagtgcgact aaactagcaa agcaaagagt
     2041 tcttttacac aatatgaaaa gcattgagac attggcaagg gtaaatgtcc tttgtgtaga
     2101 taaaacagga acgatcacag aaaataaaat gtctgttcaa aaaatgatcg tctctaaaaa
     2161 acaagaaata atcagtcagt cagatcaatt ggaagcatgc attgctgact atgcaaaagc
     2221 gatggcagga gacaatgcaa caatggaagc tgttaaagca tattttacaa aaaccactga
     2281 tcaatcctat caccatgtta ttcctttttc ttctgtccaa aaattcagca gtgtagcgtt
     2341 ggcagacaag gtgtatgttt taggagcgcc cgaaatggtt ttgcgcgatc aattgccgga
     2401 atatgcggaa gagtttgttt ctttcgctga aaaaggctat cgcgtgttgg tctttggtgt
     2461 gtacgatggg attcttgaag aaccagtatt gacagaagca gtcacaccat taggctatat
     2521 tctgattgct aatcctatca gaaaagaagc aagagccaca tttgattact tcaaaaaaca
     2581 gcaagtagcc atcaaagtga tttctggaga taatccgctc accgtatcca atgtagctgt
     2641 ccaagcaggg atttcacatg ctaatcagta cgttgatgca tctaaattat cagaagaaga
     2701 atatgaagca gctgtcgaaa aatatacagt ttttggacgc gtcaagccag aacaaaagaa
     2761 aatcttcgtt caattgataa agcgtaacaa tacagtagct atgacaggtg atggcgtgaa
     2821 tgatattcta gccatgaaag aagcagactg cagtatagcg atggcttccg gaaatgaagc
     2881 cgccatgcag gcctcacaag tggtcttact tgattctgac ttttcacgca tgccagaagt
     2941 tgttgcagaa ggacggcgcg ttgtgaacaa tattgagcga tcagccagct tgtttttagt
     3001 gaagaatatt ttctcctttt tgctctcact gttttcagtg atctttgctc tgacctatcc
     3061 tttagaacca tcacaaatca cattgattag tttgtttact atcggacttc catctttctt
     3121 gcttgctcta gaagaaaaca aaaagcgaat cagagggaaa ttcatcatga atgtgatgga
     3181 aaaagctgtg ccaggtggac taacagatat gatcgtagtc ggtgcgttgg tgatctgcgg
     3241 ggtgactttg aatctaaata aaacagatgt ttctacagca tctaccatgc ttttgattgc
     3301 agtagggttc ctcgttctct ataaaatctg ttcaccgctg aataaattca gaagtcagat
     3361 cattcttttc tgtgctagcg gtaacttttt ttcggtcatc tttttgcata agctgttttc
     3421 aatcaccggt atctcagctg tatcactttt gctgttgagc attctatttt tctcggcaga
     3481 ttcgattttt aggcatttga ccacattggt agaatatttg tttgaacgta aaaatgaaaa
     3541 agagcaaaag ccgtttagtc gcgtagctaa aaaatttcta tcgatattta aacgagaaaa
     3601 ttgacgaaaa gtatctcaaa aaagagtaaa aaagaacaac cactcgtgta tctttcgaag
     3661 caggatgagt catccaactt taaaacgcac gagcagttgc tcttttttac attttcggaa
     3721 tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc tacagtaatc gtaccattaa
     3781 ggagttgcac gtagtctttt acaatcgcta accctaatcc ggtcccgcct gaatgtcggc
     3841 ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg ttcctgatcc tcaatgtcga
     3901 tacctatacc aaagtcttgg acagaaaacg atagatcatc tgttgcctga tagcggataa
     3961 taatctcgct gtcgcttttt gaatattgga cagcattttc aatcagattt ttgataattg
     4021 gataaaggat atctgtttta gtagtaaatg gttcgttctt tccaatcaat cgaatcgtca
     4081 accgtttctt ttcgatgatt ggctgataat tttgaacgat ttgctggaaa taaggttctg
     4141 tataaattgt ctggatctcg taagaattct cgccatcttt tgacagctgg atgatttccc
     4201 gaatcagttt atctaaccgg atcgcatctt tttgcatgat ctcaagaaaa gaagtcagag
     4261 tttgcgggtc atcttttgca ccatccagta aagtctcggt aaaaccaatc agtgaagtca
     4321 caggagtttt taattcgtga gagacgttgc caacgaaatc cttttgtagc ttttctaaac
     4381 gtcgcacttt cgttaagtca tacgccaccc ctagaatctg tccggtacct tctgaattag
     4441 taaagtagcg caaactcaag tctaatgtct gccgaccttc tgtaagggtg atctcctgat
     4501 ggatcagcgg atgctcagga gtcacttgat gaatcagctg aatgagtttt ggctcttgaa
     4561 tgacttctgt gtaccgctgt tcaggttgat agcttaccac gtttaaatgt agctgcatct
     4621 ttggattaag cagtagtaaa cgagaatcag catcatcaat caggaatacg ccaatcatca
     4681 gttcgttcag taacgtatat aattgctctt ctgtggtagt acacgcccga tacatcttac
     4741 tcatttgttc gctcaacgca ttaatcgtct gatacaattc ttcccattga tcgggtgttt
     4801 gcatgattac ttccgcctga ttaggatttt gcaccatttt ttttagtacg ggcagcacgg
     4861 ttttcaatgg ctggtttttt tgatagatca gataatagat cattcctgca agaacaagga
     4921 agaaagacag aaagaagaaa aagacccaac ggcgaaaact ctcagttctc ggtaaaaaaa
     4981 cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg gccacttttt ttgactggta
     5041 atgcgacata taaaagatcc tcattcaaag tagtactttt gcgcaaagcg ctacctaggt
     5101 tcccaccatt caatacggct ttgatttcgg gacgggagtt tcgctgttca ttcaatgttt
     5161 cattgtttgt gtcaaataag atctttccag tagcatccat taaagtaatc cgttcgttcg
     5221 actgatggac gaattcttcc aaagcagttt tgttttgcgc tgcttggagg ttttcgacat
     5281 ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta gctttcttgc tgttccacga
     5341 tctgctgttg aaaataatgg gaaatcaatt gccagccacc aaaaaacaaa ctaactaaaa
     5401 gaataagaaa gaagagacgt tcataaattt ttttccattt catcgtttgg gttcctgaaa
     5461 cttatagcca aatcctctaa tagtcaccaa gtactttggg tgttttggat ctcgctcgat
     5521 tttttctcgc aaatgactaa cgtgtacatc tacgattcgg ctttgtcctg cgaagtcaaa
     5581 attccaaatg cggtctagca atgtgtctcg gtcgatcaca cggtcttttc gtttcatgaa
     5641 ataaacaagt aattcaaatt ctttcggtgt tagttcaatc agatggtcgt ctacggtcac
     5701 ttgataattg gtcaaatcag cagtgatttg tccaatcgat agatattcag gctcagcttc
     5761 atcttttgat tgcttacgag gttcaattcg tctgaatatt gctttcatac gggctaatac
     5821 ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca cctatttcta ggccaatgat
     5881 ccgatccact tggtcatctt tagccgtcag catcaaaatc ggtgtttcat ttttttcttg
     5941 acgcagccgc tgagtgatct ccatgccatc catcgttgga agcatcacat ctaaaatgat
     6001 gaaatcaaat gattgttcta atgctaattc taatccttcg cctccatctg tggcactggt
     6061 taccttgtag ccttcttttt ccaaattgaa cgtcaagagc gttacaatag aaggttcatc
     6121 atcaacaact agtatttttt tcattcaaat ctcctttaaa taggtttcat tctgtttata
     6181 ttttagcata gaatcagtac aaagggctat tttacaaaag aaataaatgg tataatagga
     6241 aggcactttg ctgaatagaa aggaagagca gtcaatgatt ggaatcagtg catgtttagg
     6301 cggtctggcc tgtcgctatg atggacagaa aaaagaagta ccagaattga tggaactagt
     6361 tgaaactgga gaagctgtca tggtctgtcc agaagtgatc ggcggattac caattcctag
     6421 agatcccgct gaaatcattg gtggaaatgg attcgatgtg tggaaagatc aagcaaaagt
     6481 atggacgaca agcggcgaag atgtaacgga agcgtacaaa aatggcgcga tcaaagccta
     6541 tcaaaaattg caggaaaaac acattgatct agtcatcatg aaggaaaaaa gcccttcatg
     6601 cggaacccat tcgatttatg acggaagttt ttccggaata aaaaaaaatg gtgccggagt
     6661 ggctactgct tattttattc agcaaaaaat gactgtgctc tctgatgaag aatggctgaa
     6721 aactagaggt gagcagaatg gaaatcgaaa agaccaatcg aatgaacgcg ctctttgaat
     6781 tttattcgac cttattgaca gaaaaacaaa tgaactacat ggaattatat tatgcagatg
     6841 atttttcatt aggtgagatc gcggaagaat acgaagtaag ccgacaagca gtatatgaca
     6901 atatcaagcg gacaagcaag atcttggagg attatgaaaa aaaactccat cttttttcta
     6961 attatattgt tcgagaacag gtattggaaa atatgactgc atatattaca gataagtacc
     7021 cggaagataa aaaactactt gaatatgtac agcaaattca agcaatcgaa gaataaataa
     7081 aaggaatgaa gaaaatatgg catttgaaag cttaactgaa cgtctccagc aggcgatggg
     7141 aaaaataaga aaaaaaggaa aagtatcaga agctgacgta aaagaaatga tgcgggaaat
     7201 ccgccttgcc ttactagaag ccgatgttaa cttgcaagta gtcaaagatt ttactaagcg
     7261 cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt ttatcacctg cacaacaaat
     7321 cgtaaaaatc gttgatgaag aattgacgat cacacttggt tctgaaacag cagagttaaa
     7381 taaatcacca aaaataccaa ctgttatcat gatggctggt cttcaagggg caggtaaaac
     7441 gacctttact ggtaaattag ccaattattt aaagaaaaat gaaaatgctc gtccgctcct
     7501 aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa ttgaaagtat taggtcaaca
     7561 gctagacgtt cctgtatttg atatgggaac tgaagtcagc ccggtagaga tcgtccgtca
     7621 agggatggag ttagcaaaag aaaagaaaaa cgactatgta ttgatcgata cggcaggacg
     7681 tctgcatatc gatgaaacat tgatggatga gttgaaacaa atcaaagaat tgacgcaacc
     7741 aaatgagatt ttgcttgttg tcgatgccat gactggtcaa gatgccgtca acgtagctga
     7801 tagcttcaac cagcaattag gtattacagg ggtcgtcatc actaaacttg atggggatac
     7861 tcgaggcggg gcagcactat cgatccgctc agtgacaggc gcaccaatca aattcatcgg
     7921 ttctggtgaa aaattgactg acttagaagt attccaccca gatcgtatgg ctagccgtat
     7981 ccttggtatg ggggatatgc tgacattgat tgaaaaagcg cagcaagact acgacgagaa
     8041 aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca ttcgatttca atgactttat
     8101 tgaacagctt gatcaagtga tgggtatggg cccactggaa gatctaatca aaatgatccc
     8161 aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta gatccaaaag atgttgaacg
     8221 caaaaaagcg atggtttatt ctatgacgcc agcagaaaga gcaaatcctg atctgttgaa
     8281 cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac agtgttgttg aagtgaatcg
     8341 aatgatcaaa caattcaagg aatcaagaaa aatgatgcag caaatgtcca aaggggatat
     8401 gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc aaaggaaaac taggcaaaat
     8461 ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag aaaaaacgga aataagatga
     8521 aaaaaacagc ttcggtacag actgaaaatg tctactgccg gagcttttct atcagcaatt
     8581 tttattcttt actaatacaa ttactctaaa cgaagaggtg gcacagatga gatgtccgtg
     8641 gagtgaacaa acagaaagta tgcggcaata tcatgatact gtctggggtg ttcctgaata
     8701 tgatgaccaa aagctgttcc gaaaattgat gcttgatatt aatcaagccg gattgagctg
     8761 gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca tatgatcgat tcgatatcaa
     8821 aaaagtcgca aattatggag aaacaaaaat cagtgaacta atggaaaaca aaggaattat
     8881 tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc aaagcagtct tgaaaattca
     8941 agaagaaatc ggcagttttt cagagtatct ttggtcgttt tctaatggaa aaatcgttga
     9001 ttcttcttat cattcacaag aagaagtccc aacaacaagt cctctttcag atgaattagc
     9061 aaaagatcta aagaaaagag gatttaaatt tatcggaagc acaacaatct atgctttttt
     9121 agaagctgtc gggatcatca atgatcacct tgacagttgt ttccgcaaag aagaggtcgt
     9181 acgtatcggc agaaagggcg aataaagcat gcgaaaaatc atattttatg gtgctataag
     9241 tttagatggc tatttggcaa cgaaaacgga tgatcttcaa tggctttttg acactccaac
     9301 aggtaagcaa actacttatg aagcttttta tgaaacaatc gatacgacca tcatgggcag
     9361 aaaaacttat caggaagcaa aaaaatattt ggagaccgaa gcaatttatc cagagaaaaa
     9421 gaatttcgtt ttttccaccc acgcacaatt acaattgcca gatgctgagg tcgtaaacga
     9481 agatcctgtt gcttttctaa aaaatctaca aatgacagaa ggaaaagata tctgggttgt
     9541 gggtggcggt cgattgatca aaccgctatt ggaacatcaa atgattgatg aatggtatat
     9601 ccagattacc cctgtattat taggggacgg gattccttta ttccaaaaag gaacatatga
     9661 agcgcgtttt gaaatgctag acacgacacg attcggtgaa tttatcgaat tgcattatgt
     9721 acgaaagtag aagtaaataa aataaaaaag gcaactatat gcagaaatgt cctgtacata
     9781 gttgcctttt ttattaccgt tatgcttttt actctgttaa tttgttagaa aaatctagat
     9841 acgcatctaa aatcgtttgg aagaaactta atgttccttc aacgatatta ccattttcat
     9901 caattagatt cgcaacgttt ccgatatatg cttccggttg ctgcaaggtt ggcacattca
     9961 aaaagacaag tgattggcgt agatggtggt ttgcgccaaa gccgccgatc ccacctggtg
    10021 atgcagtaac gactaaacct ggtttatgat cccaaacact gtgtccataa ggtcttgagc
    10081 ctacatctaa agcatttttc aatactgctg gcacagaacg gttgtattca ggagtaacga
    10141 aaacgacacc gtctagtccc ctcacttcct cacgaaaacg agtccattca gcaggagcct
    10201 gttctggagt ttcgagatcc tcattgtaaa atggcagatc atcgattttt acaaatacac
    10261 tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc tacttttttg ttgtaagaat
    10321 cttttcttaa actaccaata aaaaatccaa ttttttttgt catacgattt ttcctccttc
    10381 aaactatcta taataatctt acaaaagata agtaactgga gcaaataaaa acacttactg
    10441 acaaaagcta catagaagat agattactta tttttaacaa aaaagaagtc taaaatcttc
    10501 tattttctaa aaaaaatcga atttaatcaa gctgtaaaga aaaaaagctt tacagggtaa
    10561 aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg aggtggaaat aaataatggc
    10621 agttaaaatc cgtttaaaac gtatgggttc taaaaagagt ccgttttacc gtatcgtcgt
    10681 agctgattca cgttctcctc gtgatggacg tttcatcgaa actgtaggta cttacaatcc
    10741 tttgaaagac cctgcagaag tagttttaaa agaagactta gttcttgact ggttatcaaa
    10801 aggtgcacaa ccttctgaca ctgtacgtaa catcctttca aaagaaggcg ttatgcaaaa
    10861 acaccacgaa gctaaattct caaagaaata aggtgaccgg atatgaaaga tttaagcgac
    10921 ttagtcttaa caatcgttcg tccgctagtc acttatcctg atcaagtcaa gttagacgtc
    10981 gtagagtcga aagactttta cgagtacaat ctaacagtgg ctcctgaaga tattggccgt
    11041 atcattggaa aacaagggcg tgttgctaaa gcaatccgca caatcgttta cggtgttcgg
    11101 atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg ataaggaaaa gtaaataaac
    11161 gtgctgtact cgaattttcg agtgcagctt ttttgttttt tttattaatt gaagaaatga
    11221 ttatttggca aaacaagcaa gaaagcgtat ccttatagga ggagggagtg aacagccaat
    11281 gagaaagaaa atgttattta ttccatttgc tctattgtta agcgcttgta gttcggttac
    11341 tcaagaatca gacaatacac atctatcaag cagtatatct gaaactgtta ccagtccaga
    11401 aaaaagtagt accaaaacgg cttccaccac tgaacaaaca atggctacct caaataatga
    11461 gaaaaagact gctttagacc agttgaaaga acaacagcca aatgttccca tgcctctaga
    11521 tgtaccagtc tcttcaggct atctcaatat cgcagcgaca catacaaagc aaggttattc
    11581 tattttatat tacagaacag atcgtcccct cgggttgaat gctgacgaat tgaatcaaga
    11641 aacgcctatt gcaacctatc tatatcaata tgggtttgct tctagtcaag aaacaatcca
    11701 agttttgcag ccatttgaga tcgacacaaa tggtcagcaa gtggacttag gttctcgaat
    11761 cacagggtat cagcaaggtg cagcaggttc aagttttctt gaatggcaag aaggaaactg
    11821 gcgtattcga atccgtggga ataatataga aggccaagat cccttactat tagctaaaga
    11881 aatcgtagct tatttggaag aaaacagtct accagcacct gaacaatttg gtaaaattac
    11941 tgttgatatg ggtgatacga cgaatcgagc cgtagaagta agctggcaag aaccgaaaaa
    12001 tgcctatacg atcactcatc aagatcctat gtctgcgttg aagatggcag tttctatgaa
    12061 gaggttgtga aatcagcgcg ctgatctgag cccatcattt aactccaagt cacaggaagt
    12121 aatcaaaaat agcacgccgc atttttgcat aattttggct taatacctta aaagaaaact
    12181 agaattgcct cttcacacat gctaaaataa aacagacaaa aaagaaatgg ggatataagt
    12241 tgactgaata cttgaatgta ggaaaaatcg taaacacaca aggtatcaaa ggcgaagtcc
    12301 gtgttatctc aacaacggat ttcccagaag aacgttacaa aaaaggaacg gtgctaacac
    12361 tattccaaga cggaaaagca cctgtggaat taacagtcaa aagtcatcgc aaacataaaa
    12421 attttgactt attgagtttt gaaggacatc cttctatcaa tgatgtggaa aaatatcggg
    12481 atggcatatt aaaagtgtca aaagacaact ctgtagatct agctgaaaat gaattctatt
    12541 accatgagat catcggtctg catgtattag aagatggaac tgagttaggt aaagtaaaag
    12601 aaatcttgtc accaggtgcc aatgatgttt gggtcgttca acgtccgaaa aaagctgata
    12661 tattgcttcc atatatcgct tctgtcgtca aagaagtcga tttagaagct ggagtggtcc
    12721 gtgtagaaat tccagaagga ctgattgacg atgaagattg atgtattgac tttatttcca
    12781 agaatgtttg aaggtcctat gggggaatcc atcatcggta aggctgtaga taaaggatta
    12841 ttagatatca atatctctaa cttccgcgat tattcagata acaaacatca gacagtagat
    12901 gattatccat acggcggtgg agcaggtatg ctgctcaaag tccagcccat ctatgataac
    12961 atccaagcca ttgaaaaagc tgcaccggat gtcaaaaaaa gagtaattct tcttgaccca
    13021 gctggtaaac gattcgatca gaaaatggca gaagaatttt cacaagaaga acacttagta
    13081 ttcatttgtg gtcattatga gggatatgat gaacgtatcc gcacattagt gacagatgaa
    13141 gtttccttag gagattatgt ccttactggg ggagaattag gtgcgatggt catgatcgat
    13201 gcaacagttc ggcttttgcc agatgtgtta gggaatcaga cttcagctca aacggattct
    13261 tattcaaccg gtttattgga acatccccaa tatactcgcc cggcagaata taaagggatg
    13321 aaagtaccag aagtattgac aaatggaaat cataaattga ttgaagaatg gcaattaaaa
    13381 gaatcactaa gaaggaccta tcaacgtcgg cctgatcttt tggaaacact tgaatggaca
    13441 cctcaaatga cgaaattttt agaagaaatc aagaaagaag aacaagaaaa aacagcagat
    13501 gaagccaagt gactttttct aaaatcttct ttacagtagg ttgataatat gttagtattt
    13561 tatggtgagt gcaaaagcac ttaactatta cgatattccg ctgtgagaaa agctcataag
    13621 aatatttgga ataaggagaa gaaaaccatg aatccattaa tcgaagaatt aacaaaagaa
    13681 caattacgtt ctgacatccc agcgttccgc cctggtgaca ctgtgcgtgt tcacgcaaaa
    13741 gttgttgaag gtactcgtga acgtatccag ttatttgaag gtgttgttat caaacgccgc
    13801 ggtgctggaa tcagcgaaac ttatacagta cgtaaagttt ctaacggagt gggcgttgaa
    13861 cgtacattcc cattgcatac accacgtgtt gcaaaaatcg aagtagttcg ctacggtaaa
    13921 gtacgtcgtg caaaattgta ctacctacgt gcattacacg gaaaagctgc tcgtatcaaa
    13981 gaaatccgtc gttaatcatc gttatcagac agagaaagaa cccttggtat tcaagggttc
    14041 tttttttatt atctgttttt ttatattaac gatttctatt tggtacattt ttgattaata
    14101 aaaaggggca aaaaaggggc aacaagttac agtttttcta tatcttcttt caatttttct
    14161 tttactcctt ttgtcacatg caaataaatt ttttcagtga tatcactatt ttcgtgacca
    14221 acgcgatcct gaatggcata cagaggagtt cctagttccg ctaattttga aatatgggta
    14281 tgtctaaaaa tatgtgaact aagtttttta tcaatcttca tgtcagcttt atgacttctc
    14341 aaataggtgt taattgctgt tagttggaaa ggagttcctt tcgttgtttg aaatagaaat
    14401 tgaccatttg gatttaattc tagaagctca ttgtaaatag ctatcgcttt ttttggtaaa
    14461 tcaatttctc gcattccggc agctgttttt gtagaatcag attttttcat atcagctatt
    14521 gaacgttctc tatacatcat cgtcccattt ataactactg atgcattatt attggtaatg
    14581 tgtacatcat ctttacttaa tgcgattgct tcaccagacc tcatcccagt caaatatagc
    14641 cactggaaaa ggagagaata tcttttattg tgtgaagttg tgaaatctac tagtctatta
    14701 tattcatcat cttctaaaaa tttatcttta atttttatcg tttttgactc tcttttataa
    14761 tcaataacta cttcatcgat aggatttttt tctacataac cttttttcat agcatatgaa
    14821 aaaagaagat tcaatttgga tttaattaca ctgacgtact tatttgacaa gtcatcttta
    14881 tatatcatgt cttcaaaaat attattcaaa tctattgtat taataccaga aacgatgtaa
    14941 gtctcaggta tcttcttctt tatagtgttt aatatattat tcgtaggata atatgtagat
    15001 tccttaactt gtcttttgta tataactaac cattcttcaa caagctcatg aaatgttaga
    15061 tcaggcttat gaagtgtttt ttcgtttaat tttatatcaa tttttttatt taactctagc
    15121 aatgccactt tctgagtttc tcgagattta tttttataag taatgctcac tttttttctt
    15181 tttctagttt ttggatcaat atatcgctca ttatatttat atactttttt tccgttttta
    15241 tcagttttag tttcgatcca cattgttttc atctcctatt tgttgctata ataggaatag
    15301 ataagtaagc cgattatagc aggtttattt ttcatcacgt ccacaaactt tggcgaggga
    15361 ggggcgtgtt tttgttagtt attgtggtag gttatctaaa gcgtattgtg cttgctcatt
    15421 tgtaaaacct tcaaatataa gttgatcgta caattgcgaa tctgacattg atgcccaatc
    15481 atcgtaactt tccgctttag ccaaagcttg ttcgttgtaa tcgacatcaa cattagccag
    15541 cgcataatcg attgcatcac ttggatattc ttcaaactct agttgttcac gcaaaccttg
    15601 ttcagacata ccagcccaac ctagatagct ttcagctttg ctcaatgcat ttctgtattc
    15661 acgaggaaca ctttcttctt gagtaacttc ttctatagta gtttcagtag tttcagtttc
    15721 ttcttcaacg actgtagatt ctgctaccga ttctgcagaa gacacttctg tcacgctttc
    15781 tttagttgtt tcagcgacgg tatttttagc atcatcactg cctccttgcg aacctatagc
    15841 aataaacacc acaacaaccg ctaacaacca aaaccatacc cgtttataaa acggtttctt
    15901 aactttatac attttcccat cttgacccat aacttttttt gccatttaaa tatccctcgc
    15961 ttcttgttat aatatatttg tgatctcaga aatgaggtat gagtccgtgt tgcagcacgg
    16021 gcttttttct ttataacttt tttagagatt ataggcaaaa tagtagggca taaaaatgta
    16081 ttattgaatt ccgtatttag aaaatcctaa ttgaacttca ccggaagtct tttgctgtgt
    16141 tgtacgcaat gcttcttctt cagacattcc attctgtact ttccatgcaa caggcgacat
    16201 cccgtatttg ttaacaaaat cagtaagtga taaagtgtca gcgtcttgct gagcgcttgt
    16261 ttgttggtct tctggattct gttgagatgc tgcttgttgt tctttctgat cttgactgat
    16321 aatattgcca gcatcatctg tagtcaatcc attttcataa agggccacgc cgaaagcttc
    16381 ccactctttg ttggaccaat ttgcacgatc agctggagtt gactgtaaag tgcgttgttt
    16441 catctgttca tatgtttctt cttgaggtgc ggtttggatt gtactctgac tggagcttat
    16501 aactgttggg ctaggttccg ccgtagcttg gttggagctt gtaactgttg aactagaatc
    16561 tgttttagat gtagatttgc tagtagatga actggtttca gttgtttctt ttgttttact
    16621 tatttttgtt tcttggttag aagtggtatc tgttgattca gcttttttat tatttgaaca
    16681 agctgaaagt agcagagcag tacttaacaa caacataacg ctaacttttt tcattttata
    16741 attcctcttt ctcgttgtaa tatgtgtgct aacacgggct tttttatata agaaaacgat
    16801 aagcgctttc tggaagcccg taaagattct ttaattcctc gattttttta ggatattgat
    16861 cattatcttc tttataaaga gaaacaatga gattagcagc aaagcaatta gcttcgcttt
    16921 cagatttgct tctagatgtt cttgttgata cataatagct ggataagcca cgatgaaaaa
    16981 tagcgtgacc taattcgtga gcgcaaatgt agaatctttc ctcagagtct cgcagttcat
    17041 catttaaaaa gattatcgca cgatctctaa tttcttgaaa ctgccccttg ggattctcga
    17101 taaaaggaac gtattgaatt ttaatgccca tcttttcaca aatataaaaa ggattagcgg
    17161 actggtattt ccgcttcaac tcctcgacta aattaatcgt atccatctcc ataagctcac
    17221 atctttttgc ctttttcttt gtcttctttc acaatatccc agaaagtcgc tatcaggata
    17281 tcttttacgc gctgtatttg ttcgggtgtc aatgtttccc caccataaga catattaaca
    17341 tttgagtcta gtagtttatc aagttcaacc acttcctctt ttgtagccca cttgggaaca
    17401 ttattatttc ccaataaata atcagttgtg acaccaaaat aatcagcaac tttctttaag
    17461 ttctcagatt ttggcgaggc tttatcccat cttcttattt gtccattaga gatgcccacc
    17521 tgtctttcta cttctgctat agtcacatgc ttttcgtctg ctaattcttt aatcttagta
    17581 actaaactca ttattatcaa cctttcaaag ctgaaaagaa aataaatagc ttaaaagtta
    17641 tatttttggt tgacaattag cttttaagct agtatattta attcgtaagc taaattgtta
    17701 gctaaataag agcaacaaaa aactctacta atttaaaaca ttctctcggt cgccaaactt
    17761 agaaatgtta ttttagaggc tttttataag tcttatttaa ctatgtattc ataatagctt
    17821 aaaagctaat aggtgtcaac gatttagcta attttttagc ttacaaatta tttgtttaga
    17881 aaggagctat ttttatgtct gagaatttag acttaaaaat tcgagcggag atgagaaaaa
    17941 gaagaatgac tttcaaagaa ctagctgcgc ttatcggtat ttcaggagct tatttatcag
    18001 atattctaaa cggcaatcgt gatggaaaga aagcacaaca gcatatcgaa acagtgaaaa
    18061 aaatattgga catccgatag ggggggtagg cgatgacaaa actaaaaaaa caagattttg
    18121 taaaaaaata taattattct ccatctactt atcaacgtcg aatgtcggaa ctaaaaaata
    18181 cagcaatttt ctcagcggcg tatgaacggg tcacaggaca agaagtttgg atcaatacag
    18241 aattatacga taaatttttg tctttcaaat cctataacag gttacgcaca agaaaggtaa
    18301 cacctaaaga atttatcgag aagcatttag ttgatttata aaaaacagaa cattttgaga
    18361 ggtgaaggtt aatgggtaaa ttcaacagag cattagtatt cagcgcaccg ctaatcgtct
    18421 atgctttagg gctttgggga agtagacaag cgttgatagg aacgattgtg tacatggttt
    18481 ggatttttat agggctggat gaagctgagt acaaaacaaa aaagccagtc gggagggact
    18541 gactaatgag taaggacgat attgtatttc tgttaattat gttttgtggg attggatcaa
    18601 taataattaa tttgtatttg attattaaag aaaaattttt caattaaaat gagtctttta
    18661 gctttaactt aaattttttt tgataattag ctgttcttat gaccattaca agtttttcac
    18721 caggtaatat atctttcata tctaaaactt ctgaaaaagc gactgagtag ccaggagaga
    18781 ttgcttgtgc caacggtata ggagataaaa ggctactttt atgaatgctt cccatatcaa
    18841 aaccattggc gtgataatgt gaacaattaa ttttcttacc gttgtaaaga tacgtacatt
    18901 cgataattga gtttggcatt tttgatgggt ttgcaattac gccagtaaga atcattcgat
    18961 tacttctatc atgcttaact aaaaaatagg agagcgtaat ttttctttta tctttaaata
    19021 agttatagag ggataaaaaa aatcctgaaa tagaaatgat taaagtaata gcgacttggt
    19081 aatcgctcaa aatttttatc aagtaattca ccaccttttt actcaaatta tatcaaaaag
    19141 gagagaagaa ataatgcaag aattagtaat tttgaaaaat aaaaaaagac acgaacgccg
    19201 gcaagcatga tcgtgtcagg gaaataaact atctttctat attttaccac agaaaggaat
    19261 gaaccgtaaa tgctaattgc aacggataca ctagaccgca tctttttaaa agacgaatac
    19321 aaactgcgca atatagatgc gtcaggaatt ttagtatttg atctttatga caatggaaaa
    19381 attggtatct atcaagcaag tgatattgaa gaaacaaacc ttgctttcga acaaattgat
    19441 gattctgtgg aattggattt agatgaggca atcctagctt ttgaacaaat tgcaaaatta
    19501 ttaaaggagg cacaaaagaa tggcaactct ttaccaactc agcgagtcat atatcaaagt
    19561 cctggaactg gcagaacaat tggatgaaga aattcttcgc gatactctcg attcgattaa
    19621 tgaagcgatc gagtataagg cagaaaactt agcaaaaata gttaaagaag tagaagggaa
    19681 agctgagtta atcgatagtg aaattaaacg tctgcaggaa cgtaagacat cgcttttgaa
    19741 caatgctaag agtatcaagc actatttaca agaggaaatg gaaaagactg gtaaaacgaa
    19801 gattaaaggt gaattattca acattggaat tcaaaataat ccagtatcgg tcaatgtaat
    19861 caatgagaac ttaattccaa aaggattctt tacccctgtg cctcccaaat tggataaaaa
    19921 gcaattgaag gaggaactga agcacggaga tattcctggt gctgaactcg tccaaacaaa
    19981 aggtttgagg ataagatgat gaaggaggag aaataatgga acacgaaaac gtaaaagacg
    20041 ctttgaaagc tgctatcgaa atcgcggaag ctaaaggaat aaaggttgac ggcaaaccag
    20101 caactgtcca tgacattcaa aatttgacga aagaacatct ttattttatc gccgacttgc
    20161 ttggtttgtc ggaattgtat ttagacaaat aaccccacac ggaaggagtg gctagggtgg
    20221 ttgtattaca agtaatagtc tgcgtaacat tcatttggtc gtgcataccg gtttgtatat
    20281 atatcatgaa gaagcttcgc cctgagtctt acaaattata cgcaatttac agcataacag
    20341 tagctcttgc atttacattg tttgcaattt ctaggcttcc gttttaatcg tgggaatgaa
    20401 tttttaatga actaaattat ctataaagca aaaaaaggag aaacaacatt gaaaaacaaa
    20461 ctagcaaaaa caacagcaat catcggacta gcactaggta gcggcgttat cggctacgca
    20521 gcaagcaatg cgtttcaaga tttggacacg atcaaagcaa atttcaacac agtcctacaa
    20581 tacggtcaaa cgaaatcgca acgtgtgtca gaacttgaat cacagctatc caacaacact
    20641 cgcacacagg agcagctgaa agccgaaatt gagcaaatca aatcggacaa gcagaaggaa
    20701 attgaagcca agcaacgaga agtcgaacaa aagcaacagg agatcgctac aaagcaacag
    20761 gaagccgata gcttgcgcca acaactatcc acagtgcaaa acgacaagga gcagttagaa
    20821 cagcgcgtga gcgagttacg gcagtatacg gatcaaaagg taggggagtt ggggaaatga
    20881 aagagttgac tatttatgac ccaaatattt catgggctaa acacacaatt aaagtttctt
    20941 ttatgatttg gggttacaaa ggatatgtta cttacaaagt gggcggtaac actaagggtt
    21001 tatcattaat agctattgat tcagatgatc tatatgatgc taatttcgag gataacccag
    21061 ttaatttccg tgaccttgat gaagattggt tctctatgga acttactaac gataaaggcg
    21121 acagtacact tgtagaagat gaattcgaca gactaggtga ttatattgtc ggtgttgaaa
    21181 ttattgcgca cgaaccagag taggagagtg aatacaaaat gattaaaaga atcactatat
    21241 cagttgaaga agatagtgga tcaacaatgc agcataccga caatcgaaaa acttttttaa
    21301 ccaatagaaa ggagaatttt gatgcagatt aagaaggcga cggatatcga acaaaccaag
    21361 ggaacatatt tgatctacgg tgctccagga aaaggtaaaa cttcaacggc taaatatttt
    21421 ccagggaaaa cactgatcct tgatattgat cggacttcaa aagtccttag aggtgagaaa
    21481 aatgtcgata tcgtatatat cgacaacgaa gatacctgga acgactgggg caacacgttg
    21541 gctgatttaa ctaccaatta tgtaggtgtt tatgacaacg tggttgtcga taatgtcagc
    21601 gaattggaac ggtgtattct gtccagttta ggcgcagaag gaaaaaacaa cggcgttcct
    21661 tcgcaaggcg attatcaata tatgcaattt agaatggtga acagcctacg ctatatgaaa
    21721 aatttagaca gcaaccttat ctggaccgca tgggaagaaa tcgatttgtg gacggattcg
    21781 gatggatctt cttacaacat cgctttgccg cagatcaatc gaaagattcg aaacaatatt
    21841 ttgggattat gtgacgttgt tggtcgattg atggtaaagg aagacgatga acgcggattt
    21901 attttacaag ccacagattc aacctatgcc aaaaatcaat tggataatag atctggctgt
    21961 aagcaaagtg agctgatttt agatggaatt gtacgactac cagaatgatt tagtcgatcg
    22021 tgcaagacaa gcctatgttg atggttacaa ggcaccatgt atcgtatcac cgtgcggttc
    22081 tggaaaatca gtcatggttg cagagatcgc taaacgaacc acacgaaaag gcaacagagt
    22141 gttgttcttg gtccatcgtc gtgaattgat tgatcagatc aaagatacgt ttcaaaagat
    22201 gggcgtagat aatcagcttg taaatttcgg catggttcaa acagtcgtac gacatcttaa
    22261 aaccatcaag aaaccacaac tgatcatcac tgacgaaaac catcacgggt tagcagcgtc
    22321 ctacagaaaa atctatgagt attattcgga cgttccgcga ttaggtttta ccgcgacacc
    22381 catccggtta aacggtagtg ggttggggga tgtgaacgac ttgctaattg aaggagtatc
    22441 ggctaaatgg ttgattgaga atcatagatt agctccttat gaatattatg cacctaaatt
    22501 gatcgataca gccgaattaa agaaagcttc gactggagat tttacaaaga aatcaatgga
    22561 taaggcagtg aagaatacta tttatggcga cgtgttgaaa cattataaaa cgttagcaga
    22621 aggtgaacag gcaatagcat actgtcacag tatcgatgca agtaaacaca ctgctgaaat
    22681 attcaacgat gctggttata aagcaacaca tatcgatgca aaaactccaa aagatgaacg
    22741 tgcagatatt atcgaagcgt tcagaaatca tgaaattaaa atcctatgca atgttgattt
    22801 gatcggagaa gggtttgatg ttcctgattg ttcgactgta attatgttga gacctactca
    22861 atcgttatca ttgtacattc agcaatcgat gagggggatg cgttaccgac cagataaaac
    22921 gtcaatcatc attgaccatg tgggtaatgt gaaccgtttt ggattgcctg atatggatcg
    22981 tgaatggagt ttagatacaa aaaagaaaac aaaaactgat agtgatttat ctatcgttca
    23041 atgcgcgttt tgttttggtg cttacgaacg gccaaaaggc gataatattt gtccgtattg
    23101 cggagagttg cagccgattg aagaacgaaa gtcagagtta gaaatcgatg aatcggctga
    23161 attaatgaaa gtcggagaaa cgaaaattac tttgaatttt gagaacaata aatattacaa
    23221 catgacggaa gatgaagcag aaagtataga ggacttatat gccatagcaa gagctaaagg
    23281 atttaaacct ggttgggctt atatggctgc taagaggaag gggtggttgt aggaatgggc
    23341 ggtatttttg gtaaaaaagc agaagatgtt caaggttcaa taataaatgg cgtaacaata
    23401 ataggtgata ctggaaaaag agatagttca ggcaatcaaa ttgtaatcgc tagaaataaa
    23461 aataacgaag tttttgaatc ttctctatct aatataaaaa gaggtgttgt taacgggaca
    23521 ggcgaagaga gtggctacgt taatttaaaa aaagctaatg aaaaatacca tgttgaagga
    23581 acttacgtta aggctttgtc tgtcggtgga acaaaaggcg ttaattggga taaacgaaga
    23641 aataaatgga aagcacgatt atatttcaaa ggagaatatg tcttacaaaa ggatttcaac
    23701 accaaagaag aagcaatcca agcacgactt gcagcagaag aaaaatattt caaacctatc
    23761 ttagaaaaat atgaaaataa aaaggagaat gattaacatg acaggattta acttagactt
    23821 ttcaaaagcg caacaaggga acgaaatcaa agacggtacg tacgaagtgg tcgtaaacaa
    23881 agctgtagaa aatgcaacta aatcaggcgc agaatttatc gatatcgatt tgatcgttcg
    23941 taacgatgtg gatcaaccgt tccaaaataa acacattttc gcaaaaatct ggaaagccaa
    24001 agctaccggc aaatataacg aaggtatgat catggcaatt gcacaagcgc tacagttgga
    24061 agacggaaaa tcatataacg ggtttgatga attgctggcg gactttgttt tgaaaaccgc
    24121 ctcagtacgc gtgaaaactg aagagtcaaa tggatataaa aacgtcaatg tgaagtcttg
    24181 ggataaaacg aacacacggg gcgtaatgaa tcaccaattc aaaaatggtg atgaaccatc
    24241 cttcggaccg gaacgttcaa gagcgacaac ggttagaaat gacaatcttc cattttaatt
    24301 gaggtgtaat caatgtatga aaaaattcca aaagagctta aaaatctcaa acaatggtgt
    24361 gtttataagc tagtttggga tgaaaagcgg cagaagcaca cgaagatccc ttataacgcc
    24421 aacaacgggc ataaagccaa atccaatgac gagagtacct ggtctgattt tcagaccgct
    24481 ctcgctgcta ttgatcacta cggaatgagt ggactggggt tctttttcaa accaccttat
    24541 ttcggcattg atatcgataa tgctgaaggt gaagttgagc gatttaaatc aggtgatata
    24601 gaagaaaata ttatctatga atttatcgaa agcatgagat cctatgctga gtattctcaa
    24661 tcaggtactg gcatccacat tattgctcgc ggagaattgc ccggcggaag acgcaggaaa
    24721 ggtgacgtag aaatgtacca gaacggccga ttcttcgtta tgactggaaa tgccgcatca
    24781 aaatatttag aaataactga gccgaatcca aaggatatca aacgcctata tgatcgctac
    24841 gttggagatg aaaaaatcat ccaattcaaa gaagaaaatc cgctgatgaa taccgttgat
    24901 ttaccggttg aagaaattat caaacgtgct gaatcgtctt cccaaggggc aagattcaaa
    24961 gtctttatga acggcggttg ggaagctttt tattcctcgc aatccgaagc ggatatggcg
    25021 ttcgccaacg acttggcttt ttggaccggt agagactttg aaaaaatgga tgagattttc
    25081 agagcgtctg caatgatccg tagcaaatat gaccaaaaaa ggggatcggc aacttacgga
    25141 gaatctctac taaataaggc aattgctgat acaaattccg tctacaaccc caaacggaaa
    25201 tctgatttta agatttttat caaagaccag gaacagccaa aagaggaaaa atactattcg
    25261 tacgacgaca ccggcaacgc ggatcggttt actgatatct acggaacgtt agtcaaatat
    25321 tcgtacattg ataaatcttg gtattactac gatgggaaag tttggctaca ggacaatacc
    25381 ggcgaagttc ggaaaatgat cgatacgaca gtagacatca tgggaaaaga accgttaacg
    25441 atccctgaag gcgcagacga tgaaaccaaa gaagctttga tgaaagcgaa agaaaaacac
    25501 gtcaagcgtt ctcgcagcaa tgccggaaaa aacgctatga tggacgaatt gaaacatcga
    25561 ctatccgtat tgccggaaga gttcgacaaa gataaaacgc tgttcaacac acaaagcggc
    25621 tatctgtctt tgcatgacgg tcttctacac gagcatgaga tagacaaaat gtttacacgt
    25681 gtttcaaatg tcgagtacac agaatcggtc gattgtccta tgtgggaaga atttattaat
    25741 cagatttttg acaatgatca agaattaatc agatatatcc agaaatgtgt cggatattcc
    25801 ctgaccggat ctaccagaga gcaatgtatg ttcatcctct acggtcatgg gtcaaatgga
    25861 aagtcggtat ttttggaaat tatttctgaa ttgatgggaa actatgcgat gactatgcag
    25921 gcacaaacga tcatggtcaa gcaaagccaa tcatcagcaa acagtgatat tgcacgattg
    25981 aaaggtgcgc ggttggtcac ttcgtcagaa ccgaatgaag gcgtccgact ggacgaaggt
    26041 ctggtcaaac aattgactgg tggtgataaa gtcacggctc ggcaccttta tggaaaagaa
    26101 ttcgagttcg aacctgaatt caagttgtgg ctggcaacga accacaagcc catcatccgt
    26161 gggaccgatg acggcatctg gcgacggtta aatttaattc cattcactgt ccaaatcccg
    26221 gatcacaaaa aggataaaaa tctgaaattt aaattgcaga cggagttgca agggatcctt
    26281 aaatgggcaa tcgatggttg cctgttgtgg cagagagagg gcttggagaa accaaagtcg
    26341 gttgttgcag cgagtcagga ttatcgaaac gaaatggatc aaataggtac atttatcgaa
    26401 acgtgttgcg aaacaggtcc aggactaaaa atatcaggcg gagaattata caaagtttat
    26461 cgtgaatggg catcagataa tggcgagcat actttcacta acacgaagtt tggcagagag
    26521 atttcaaaga aatatagcaa agaaaaagct ggtggattta tggtttataa aggaatcaca
    26581 ttaaaaccaa gaaaatacga taacgttaga gaacttttta aataaatctg gagggttctg
    26641 gagggttgag aacgaaaccc tccaaccctt agaaacgttg atatatcagt atttatatta
    26701 tatttttctt tctttctgga gggttttaaa aaaataataa agtattaaag taaaaaataa
    26761 aaagtaaatt atataaaaaa gaataggttt ttgggtctga ccctccaaac tctccaccaa
    26821 aatcttccta aatgctttga taccaacgat taaacagtct ggagggttac attgaaatca
    26881 gaacatcaaa tacaagacga gatcagaatt gccttatcaa aacatggttg cacgattttt
    26941 cgatccaatg cgggacaagt gacaacgatc gaagggcggc aattttacgg gatgccaaaa
    27001 gggttcccag atctatgtgg acatcgtaat tctgatggga aagcaattta tatagaagta
    27061 aaaaatgaac gcggtaagtt gcgtgaagat caaaaaaggt ttgctgaatt tttaaacggt
    27121 caacctgtac tatacggtgt tgcacgatca gtggaagaag cattgaaaat tgtggaggaa
//
LOCUS       DACECR010000030        27048 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.030, whole genome shotgun sequence.
ACCESSION   DACECR010000030 DACECR010000000
VERSION     DACECR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.030"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(84..1778)
                     /locus_tag="IWO17_001199"
     CDS             complement(84..1778)
                     /locus_tag="IWO17_001199"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ0290087.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            complement(1835..1963)
                     /locus_tag="IWO17_001200"
     CDS             complement(1835..1963)
                     /locus_tag="IWO17_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ0290088.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            1962..2210
                     /locus_tag="IWO17_001201"
     CDS             1962..2210
                     /locus_tag="IWO17_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290089.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2402..2767
                     /locus_tag="IWO17_001202"
     CDS             2402..2767
                     /locus_tag="IWO17_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0290090.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            2764..3129
                     /locus_tag="IWO17_001203"
     CDS             2764..3129
                     /locus_tag="IWO17_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0290091.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(3221..4648)
                     /locus_tag="IWO17_001204"
     CDS             complement(3221..4648)
                     /locus_tag="IWO17_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0290092.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            5118..6953
                     /gene="tdc"
                     /locus_tag="IWO17_001205"
     CDS             5118..6953
                     /gene="tdc"
                     /locus_tag="IWO17_001205"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0290093.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            7258..8736
                     /locus_tag="IWO17_001206"
     CDS             7258..8736
                     /locus_tag="IWO17_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0290094.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            8702..10033
                     /locus_tag="IWO17_001207"
     CDS             8702..10033
                     /locus_tag="IWO17_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0290095.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            10147..12699
                     /locus_tag="IWO17_001208"
     CDS             10147..12699
                     /locus_tag="IWO17_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ0290096.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(12706..14145)
                     /locus_tag="IWO17_001209"
     CDS             complement(12706..14145)
                     /locus_tag="IWO17_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0290097.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            14491..14853
                     /locus_tag="IWO17_001210"
     CDS             14491..14853
                     /locus_tag="IWO17_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290098.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(15040..15897)
                     /locus_tag="IWO17_001211"
     CDS             complement(15040..15897)
                     /locus_tag="IWO17_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0290099.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            16017..16928
                     /locus_tag="IWO17_001212"
     CDS             16017..16928
                     /locus_tag="IWO17_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0290100.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            16935..18581
                     /gene="mdcA"
                     /locus_tag="IWO17_001213"
     CDS             16935..18581
                     /gene="mdcA"
                     /locus_tag="IWO17_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ0290101.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            18683..18982
                     /locus_tag="IWO17_001214"
     CDS             18683..18982
                     /locus_tag="IWO17_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ0290102.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            18972..20648
                     /gene="mdcD"
                     /locus_tag="IWO17_001215"
     CDS             18972..20648
                     /gene="mdcD"
                     /locus_tag="IWO17_001215"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ0290103.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            20652..21245
                     /locus_tag="IWO17_001216"
     CDS             20652..21245
                     /locus_tag="IWO17_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ0290104.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            21269..22345
                     /locus_tag="IWO17_001217"
     CDS             21269..22345
                     /locus_tag="IWO17_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ0290105.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            22370..23239
                     /gene="mdcB"
                     /locus_tag="IWO17_001218"
     CDS             22370..23239
                     /gene="mdcB"
                     /locus_tag="IWO17_001218"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ0290106.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            23350..24114
                     /locus_tag="IWO17_001219"
     CDS             23350..24114
                     /locus_tag="IWO17_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ0290107.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            24603..26696
                     /locus_tag="IWO17_001220"
     CDS             24603..26696
                     /locus_tag="IWO17_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0290108.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            26721..27023
                     /locus_tag="IWO17_001221"
     CDS             26721..27023
                     /locus_tag="IWO17_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0290109.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         8680 a         4633 c         5642 g         8093 t
ORIGIN      
        1 aaccaaatca aaaacataag aagctgtttt agcctggttg ttcttattac ttgatgcagg
       61 gcagcttttt cacaacctct tgattattca aacatttttt ttgcggcacg agctagagta
      121 ggaatatctt tgtcgtaacg cggtgttttg acaagacggg acataggaat acccgcaaaa
      181 tggaaagcag cgatttctcc agaacgtaca gcactttgtt cagtaaagat catctgataa
      241 ggctgctcga caaattctcc tgtaaaggcc agattggtcg aatgtttagg aatgactttt
      301 ggtcgatcgc ttttggcacg attattgaac aacgcggaag cataaggcat atagacaggg
      361 atattattta ttacgctgtc gaaaatttct gtttcttttt cacgaatatt cactggtcca
      421 ggatctactt ttgaaagttg tccaatcagt tcttctagca tttcttttcc attcatttta
      481 atatactgtt tatccacaaa ttcgccacgt cgtcttggat aaaggaaata accccaaata
      541 actgtttcat ttggtttctg agacgtaaaa tgcggttgat gatgcacgac aatagacata
      601 ttgacatctt tttggcctaa cggcgtgatc ggtgtggttg aaataaatga attcaaggca
      661 tttcctggtt cttgagtcgt gatacgggtg atttcattca acaacaaatg gtttttcgtc
      721 gtcagggtaa agctgaccca ttcgcttgca tctcgatcag caaagaattt gtcaggattt
      781 cccaagttgt agaaacgttc actggctttt ttccagagac tagaagctgc accataatcc
      841 atattttcta cagctggtgt ttcgtaatct cctaaagtag ctgaatctgt aatggaccca
      901 ttagtgaaaa taactgctgt atcttcatcg accacaacgt attcttcttc ttgagtcagt
      961 gtattggtaa ttttcaaacc agtcacagtg atttcatctt gcatcggtgt ttctttgaat
     1021 tcccagtcag tgacacggcg gttcaaaatg attttgcatc cttgttcttt caagtactcg
     1081 atcaaaggaa gcataatact ttcgtattga ttgtaacgtg ttcgattgac gcccactaga
     1141 tgttcgattt gagtgaattc ataaatcatt tgatgcatat agcgacgcaa ttcttgagca
     1201 gaactacgtg ttctgaaagc aaatgttgtt tcccacataa accagaagtt tgtttcaaaa
     1261 aagtgtggat catctttgaa atattcttcg atagtgatat tgtctagttt ttcttcttct
     1321 gaatcaggca tagcaatcag tttagtcaga agaatacggt ctttattatt gaatcccagt
     1381 tttccgccat caatgattcc tttgccacct tccatcaaac gtgctttgtc aaaggttcgg
     1441 tgttttgcat caaaatctct cgtatcttct tcagcagtca tccctggctc agtaacagaa
     1501 ggtattcgac ttaacagatc catcaaatct acataggtac ggtaattaag catgcgaccg
     1561 cctcgagcaa tatagccttt ttggttttct aatggatggt ttttattcca atattcatcc
     1621 actacagtgt cggtcggtgc accatcattt gaaccgtgat catccaatga ataaaaggtg
     1681 atctgatccc ctttccattt gccttcttga atcaaataaa cagccgctgc catattggca
     1741 ataccggcac caatcattat tgctttactt tttctcattg tgattcctcc aatgtcttcc
     1801 aagtatgtcc gtagtgattc aactaaatag gatgctagat tgattcgaat tcatcatata
     1861 aatgactgtc atcttcgaat agtcctgtct tttttcctag atatgcagcg ccaagggcaa
     1921 gcaaacctaa accgatgccg actttgatta attttttact catgaaaaac gcctccttgt
     1981 tttctctttc tatatccaag gatagttcgt ttttattaga atgccaaatg ataggacgag
     2041 aagaaaaagg aaattttgac aaaaaagaag aagtgtctga ttttcctgta aataaaaata
     2101 gggtaggtaa gaagaaaaga tttgaggagg tgtatggttc tatttttaga aatttgggta
     2161 caaaaaaagc accgagacag tgcttctttg aaaagctgat gacggactaa gccttatttt
     2221 aacttgctac gttgttttgg atacttccac gatttatgga tggagggaga aaaatgggag
     2281 agtgaaaaag aaaaatttaa gcgaaccgtt atcattagaa caacttttaa atagacgaac
     2341 aaggtaactt tcttttacaa taaaagaata ggaacaaatt aaaagaaagt aggtagcgta
     2401 tatgtttcgc tcaaatttaa ttgaatttct agaatggatc attgctattt taaatatttg
     2461 ttctatcggt atcttaattg tcgggatcgt tcttgttcta ttcaaacttt ttgattggtc
     2521 tcatgtcaag aaaaactatg cagaacgtaa tactcgtaat gcagatacaa gaaaactgct
     2581 agcaagttat attttgttaa gcttggaagt actagtcgtt gctgatatca tcgagtcagt
     2641 tatcaagccg acttggacag atattttgaa gcttgcactt attattatca ttcgaacagt
     2701 catttcttat ttcttgaatc aggaaataga agacaaagaa aaacaaagaa gaggagaggt
     2761 aggatgacga cagagttgat gcagtttctt cttccatatt tcgaaaaact tgtactggga
     2821 cttgatatct ttgctatctg tttattggtt tggggtgttt gtctatcaat caaggatttt
     2881 ttgaaatatt tatttgtcca tacaaaagtg caaagcgacc gagtcagcga gaacaatgct
     2941 attaaaaaga tgttgggagg ctatatactt ctaagtttgg aaattcttgt ttcagctggg
     3001 atcatcgaat cgattatcaa acctaccttg caggatattt tccaactagc ggcgttggta
     3061 gttatccgaa ccatcatttc ttatttttta aataaagaga tcggacccgt agatgtccaa
     3121 aataagtgag gttgtgagcc tgacgtgtat caagagattg ttacagaaat gggcatcttc
     3181 aacaaataag aggctgtgac atgatttttg tcacagcccc ttatttgttt tgcacagctg
     3241 aaatgatagc tcgatcgatc gtattgatcg aaataggaat aatattttca tattggaccg
     3301 catctggtgt atataatgta cgttcacata atacccggtt tgaggactgc tgcgcatact
     3361 tctttatatc agatatactt gaataaattg ttggtgcaac ttgaaaatcg aaagtagtta
     3421 cagcttccaa ggcttcccga taaatcagat aatgaacatc cgaatttgtg acgatataga
     3481 tagtcagatg ttcttttgtc agacggagaa ttccgctcaa ttcgatcaca aaagcccgca
     3541 tgcaattagg attcaaccgt agatcataag gtaactggct ggaccagctg gaaaaaattg
     3601 tctggacttc tttatataaa tttgtctgtt cctcatttaa aagataaaat ttttctggca
     3661 tgaataattg atagttatcc caagccgaac gcatcagatg aatcagcgca cgttcgaacg
     3721 gttggtttcc gaataattct tgattgaagt gagattcaaa ggtagcaact agttctttta
     3781 tttcgggagt attgtcaata aacacttggt gtagctgttg aaagtcttcc gcaatcaact
     3841 gataagaatg gaaaccataa ttacaaagat tgaatagtgt caaaataaag gcgaattctt
     3901 cttttttata ataggtttgg aaatcagtgt tttcccatgc tgattcgaca taattgtaaa
     3961 taggacgttt tttgatttct tcgataaatt ttgaatctat tgtcacagca cacacttgct
     4021 ggcgtaggaa actgatagca aaaccaatcg ataagatttc tttattttct ttatcataaa
     4081 atctgccact attttccgtt acggattcaa tgaaacgatc ggcacgttca aaaaacgatt
     4141 caggtagctc ccaagaatta aaaccgccta aacgatacga cacattaaga agcagcagac
     4201 gtctttccat ttcagtgaat cgaggggagt agcggtcgat ttcgatcccg cagtcattaa
     4261 aataagctaa tacttgtttt tttaatgaat aagctttgct gacagagatg aattcacatt
     4321 cagttaattg aagataatca ggttcatctt cttccaaata aagcagacaa gtttttaaaa
     4381 acaacgaatt ttgagacaat tgctgcatga tcttgcatcg aggtatatgt tctgaaaaat
     4441 ataaagtgta acggtccttt tcccgatgtg tataagcgat ctgttcttca aattcttttt
     4501 ttatgttatt gatatcattg agtaaagtgg ttttgcaaac atgcaggaga ttggagagct
     4561 tttctagatt gacttcttta tttacataga attgcccaca taaaaacagc tgtcttaaaa
     4621 tatttttctc aatatattct tcaatcatag tagagcctcc gccttgtatt tttttgcaca
     4681 atatgggaaa tattatgaca tgttttcttt ttgaaatcat cagttaattt ctgaaaaagc
     4741 atataaatgc cgttaaaaaa ggttttttat gttataaaat ttggaaaaca gataattcta
     4801 ttaaaaaata aattgcgaat ttttaacaat ataaaaaaat aaaaatatgt ttagtgaatg
     4861 aaggacagtc attttatttg tttgttcgct ttttttaggt tatctatttt agaagactga
     4921 tttattgaaa aattgttcgc ttttttgcgt tgaattatca gaaaaatccg actttattta
     4981 tttttgtttg taaaaatcta cgtggatttg atgttataaa aaaaaaattc tcatttttgg
     5041 cgctatagtg aagaggtgtt acagataaac acatgtatct gcaagacatt gaccaacaaa
     5101 atatggaggg aagaaaaatg aaagatatgg atatcaaggc cgtctttata ggcgacaaag
     5161 ctgaaaatgg accagtttat aaaatgttgt tgaacaagat ggtagatgaa catcttggat
     5221 ggagagaaaa ttatattcct tcagatatgc ctgcaattag cgaaggagat aagctcactc
     5281 cagattatct agccacccgc gaccatatga tagaggtctt ggatgaagtc agtcagcgat
     5341 tacgcgcagg atcgatccca tggcattcag caggccgtta ctggggacaa atgaatgctg
     5401 aaacattgat gcctgcttta ttagcttaca actatgcgat gctgtggaac ccaaataatg
     5461 ttgcattgga atcttctatg gcaacttcgc aaatggaagc agaagtagga caggattttg
     5521 cttcattatt caatatgaca gacggatggg gacacattgc agcagatggt tctattgcta
     5581 atcttgaagg attatggtac gcccgttgca tcaaatcgat tcctttagct gtcaaggaag
     5641 tgctgcctga aaaagtgaaa aaaatgtctg agtgggaact tttgaattta tctgttgaag
     5701 aaattttgga aatgaccgaa agctttacgg acgaagaaat ggatgaggtc aaagcagctt
     5761 cttcccgtag cggaaaaaat atccaaagat tagggaaatg gctggtccct caaacaaaac
     5821 actattcttg gatgaaggcg ttagatattt gcggagtcgg cttagatcag atggttgcta
     5881 tcccagttca agaggattat cgaatggata ttaatgcttt ggaaaaaaca attcgtgaat
     5941 tagctggaca aaaaatccca attctaggtg tggttgctgt cgtaggaacg acagaagaag
     6001 gtcaagtaga cagtgtagat aaaatcgtcc aattaagaga aaggttaaaa gatgaaggga
     6061 tctatttcta tttacatgtt gatgccgcat atggcggcta tgcgcgttca ctgtttctaa
     6121 acgaagcagg agaatttgtg ccgtatgctt ccttagctga attctttgaa gaacatcatg
     6181 ttttccacca ctgcgtaacg attgacaaag aggtatatga agggttcaga gctatttcag
     6241 aagcggattc tgtcacgata gatcctcata agatgggcta tgtcccttat gctgctggag
     6301 gaatcgtaat caaacataaa aatatgagaa atatcatttc ttatttcgcg ccatatgtgt
     6361 ttgaaaaatc agtaaaggct ccagatatgt taggagctta tattttggaa ggatcaaaag
     6421 ctggagcaac cgccgccgca gtctggacag cacatcgtgt attgccattg aatgtgaccg
     6481 gatatggtca attgattggg gcttcaatcg aggcagctca aagattcaga gaatttctcg
     6541 atcatctgac ttttactgtt aaaggaaaaa caatcgaagt ctatccattg aatcatcctg
     6601 attttaatat ggtcaattgg gtattcaaag aacaaggctg tacagatttg aacgctatca
     6661 atgaattaaa tgaaaaaatg ttcgatcggt cttcttatat ggatggcgat gtttatggtg
     6721 aacggtttat tacttctcat actacattta cacaagaaga ttacggtgat tctccgattc
     6781 gttttgttga aagaatgggt ctaacgaaag aagaatggaa aaaagaacag aaaatcactc
     6841 tgctacgtgc ggctatcatg actccttatt tgaatgatga tcgaattttc aatttttata
     6901 caaaaaaaat cgccaaagca atggaaaaga aactaaacga aatcatccaa tagaactgat
     6961 ttttgtgtga aagaataaca agagacatat ggagaacaga caattatttg cttcatcaaa
     7021 acaattgtat ttccccctgt ttttcatata ttcctcgcca agatattctt ttttataaaa
     7081 cgttctataa gttggatcgt tcgtttcatg tatcaaaccc agttcactaa gatcgttcaa
     7141 tggatgtttc ggtgatactg ggttttgtac tgaatcgatg aaaccttgtc atttctgttc
     7201 ttgaacaaaa ataaaagaaa acaaaaacat atgtcgctat tgaaagaagg aataaagatg
     7261 aaagatactg gttcatcaaa gcaaatcacg accatgacct ttatcggaat gacctgcgcc
     7321 cttgtagcaa gtgtacgaaa tattccagat gtagcagcaa cgagctggac gatgattttt
     7381 tacatgctgg ttgcagtatt attgtacgct tttccaatct cattgatctc tggtgaattt
     7441 gctggaatgt ttccccaaaa aggtggtcca gaactttggg tatcgaatgc tctaggaaaa
     7501 aaatgggggt tcgttgtctc atggctgtta tgggtccaaa tgtttcctgg gatggtgatg
     7561 gtcgcttcag cactcgctcc gttgtttggt aatattatcg ataatgtgcc gcttggtttg
     7621 aacagcaaat ttacattagt tgtcatttta gtcgtttatt ggatcatcac ttttttgaat
     7681 ttgaaatttg atatggcaaa aatcggcgga aaagtcggtg tttggttagg attgtacgtg
     7741 cctttgacga tgatgctctt attagggttt gctgcgtgga tcaaagttgg gatcgtacca
     7801 accggaacat taggcagttt ttcatgggac aaattaatac cagatactac gactgcatcc
     7861 tcatttgttt attttgcgcc gatcatgttt atttttactg gtatcgaaat gtcctctgtg
     7921 tatatcactc gattggaaaa tccagttaaa acgtatattc gcggagtatt tgccgcgtta
     7981 atttttatat ttttcgtcaa tattttgaat gctttagttg tagcaaatgt cgtaccaaaa
     8041 ggacaaatgg aattgaataa tatcgcgcaa tctatttcga tttattgcca aattcttggt
     8101 ttgccacatc ttattgtgaa tctgtttagt ttacttgtct tcattggtgt agcagtccaa
     8161 ttatctgctt gggcctcagg accagcaaag acagtgacag aaagtgctag aaaaggtgcg
     8221 tatccaccaa aattcaattt ttggaagacg aatcaatttg acgtatccaa atccgttatt
     8281 ttgactcagt ctgtcattat ctcaatcttt gcactttttt atttgctgat tcctggcgtc
     8341 aatcaagcat ttttgatgct cgtaaattca actactgtta tctactgtat cgtctatgtc
     8401 atcatgggga tcgcagtttt gagattgcgt tatacacatg caaagttgaa tcgaccattt
     8461 agaattggaa aaaaagagaa aaagagcaat cttggagttt ggactgtcgt tattgtttta
     8521 tttgcggcaa tcggattttc tgtcggactg acgatgaaag ctggaacatg gatcaatcta
     8581 attgcagtca cagccatcag tgtcattttg tttgtagtac cactctgtat tgaaaaaata
     8641 aagaaacctt cttgggaaca ggaagtcgcc atgctgcttg aaaaagaaaa aggtgaactt
     8701 gatggaaaaa ccggcaatga aacggactct aactaatcga aatatccagt taatcgcatt
     8761 aggtggcgca attggaacag gattattttt aggttcagca aaggcaattg catcagcagg
     8821 gccagctgtt attctcgttt atttgatatg cggtttggct atttatggtt tgatgcgggc
     8881 catgggtgat ttatttttag caaatagtac atttaactct atcagtgatt tcgttgacta
     8941 ttatttaggc aaaaagtggg cattttttgt caattggact tattggcttt gctggcttgg
     9001 agctggactt gcggaattaa cagcaatagg attgtatatc catttctggt ttgtacatgt
     9061 accagctttg ataagcggag gtagcgcact aatgatctta atggcagtca accttgttgc
     9121 agtcaagtgg tttggtgaat tggaatcttt tttctcaatc atcaagatct tggggatttt
     9181 cctttttatc ttattgggta taggattgag tgtctcagat tttcataccc agtctatcgg
     9241 aatcaaagaa aattttggac agatcgatga attactaccg tttggctttg ctggaatggt
     9301 agtctcattt cctatggttt tattcagttt tgctggaatt gaaatgatcg gcttaaccgt
     9361 tggcgagaca agtgatccgc aaaagaattt gaaaaaagca atcaactgtc tgccttggcg
     9421 tattctcttt ttctatatag gaacgatttt gtcgatttta tttgttgttc catggcattg
     9481 tttagattta aaagaaagtc ctttagtgac tatgctgcaa gtgatccatt gccaaggcgg
     9541 agcagcaatt gtcaatctaa tgattttggt tgcttctttg tcctcagcaa atagtgcagt
     9601 ctacagtact agcaggattt tatatcaaac agcaaaagaa aaaagtttgc cccattggtt
     9661 ttgtgcgctt tctaagaaag gcgtaccttt acacggtatt ttaattacag gaatgttatt
     9721 ttttgccggt ctttgtctac atttttttgt agctgatagt cgattgtttt ttcagctttt
     9781 ggcaggcatg acaacgagtt gttttctatt tgtgtggtct tcgatcttgt gtgcacatgt
     9841 tcaatttatt aaagaaaaga aacggaacac tacacaaaga tataaagatc ttgctttgtt
     9901 ggtgtttttt acttcaattg ctttcagctt gttattcgaa agcacgacac gctttattcc
     9961 ttttttattg gttatctggt ttgtattctt aaattttgta tataaaagaa taagcaatag
    10021 aaatatcagt taggcccaga tacattttgc aaaacgaatt atttcagacc cccctgaaat
    10081 aatcgatacg cctgaaaaaa cttccctgcg tataataaca gtatcaatta aacaggaggg
    10141 gataggatga actggtacag attgacggta gaagatgtat tgaaaaacta tcaaacatca
    10201 acagaaggct tgtcgcaaga agaacgacag aaacgcctga ctgatcaagg atataatgaa
    10261 ataaaagcaa ggcagcagac aaaacgatgg aaaaaaatag caaagcattt tactgatctg
    10321 ctgatgatcg tgttgatcat tgcctctttt ttgaaatttg cttctagtga atatattgaa
    10381 ggaagtatta ttttattcgt agtgatcgtc aatggactcg ttagttactg gcaagaaaga
    10441 aaagcggaag aatcattgaa tggtttgaag cagctgatgg gacaagaagc gatagtacta
    10501 tcaaacggga tgcaagaaaa aatccctgcg aaagaactgg taaaaggaga tgtcattgtc
    10561 ttacgtgccg gagatgtcgt tccagcagat atccgattaa tcgaagcaca tgatttatta
    10621 gtggaagaat cgattttgac gggagaatca gaagcatctg aaaaaagtca tgccgttttg
    10681 acagaagaag aatcgatcgg cgaccagaaa aatatgggtt tttcaggaac gcttgtacaa
    10741 tctggttctg cagtcggtgt cgttgtagaa acaggaaaca cgacagaaat cggtaaaatc
    10801 aatcaagcat tgcaatcggt cgaacaacaa acgacgcctc tgattaaaaa aatcaaccga
    10861 ttaaacaaac aaatttttca aggcattctt tgtttgagcc tattcttagt tatttttacg
    10921 acatttcgat atggtatgga ttggcatatc cttctttcgg ctacgattgc tttggtggtg
    10981 tcaatggtac ccgaaggatt gccagctgtt ttgaccatga ttctatccgt tggagtacat
    11041 gaaatggcaa aagaaaaggc gatcatcaaa ggacttcctt cggtagaaac attaggttct
    11101 atgacagtga tctgttcaga taagacagga acattgacaa aaaatgaaat gacagttgtg
    11161 gatgtagctg caaaagaaga agcctgcgtg ttgtccatta tgaaaaactg tcaggaatta
    11221 aaaacaaaag aagaacagaa aacagaaaat ttaagtggca atccgacaga agttgcgctg
    11281 attcgacaca cagaaacagc caatctacct ttgaaaaaaa tagaagcaaa gattccgttc
    11341 agttctgaat acaaatatat ggcaacgatg catgcggagg aaaaaggagc ggtgatttac
    11401 gtcaaaggtg caccagaagt attatttgct aaatcaactc tttcttctgc agaacaagaa
    11461 gcatggagtc agacagctgc agaatttgcg agaaaaggtc agcgtgtttt aggatttgct
    11521 tataaaaaag tagattcgaa acaggaactc acgcatgaga ctttaaccca attaactttt
    11581 gcagggatcg ccggattgat cgatccgcca aaagaaagtg cagtcaaagc tgtaaaggaa
    11641 tgccaacaag cagggatctc agtgaaaatg attacagggg atcacaaaga cacagcaaaa
    11701 gcgattgcgg aacaaattgg cttaaaacat actgccaaag ttttagaagg gatcgatttg
    11761 gatcttatgt ctgatgaaga gttgattcag caagtgccaa tagtcgatgt ctttgctcga
    11821 acgacaccag aacataaact gcgaattgtt aaagctttgc agaaaaatgg agaaatcgtc
    11881 ggaatgaccg gagacggcgt aaatgatgca cctgctttga aaagatccga tgtaggaatt
    11941 gctatgggaa tcaaaggtag tgaagtaagc aaacaggcag ctgatatggt tttaggagat
    12001 gataatttcc atacgattgc aaaagccgta aaagagggcc ggcggatttt ggataatttg
    12061 caaaaaacca tcaatttctt cttgccaact gcattagctc aaggattgat cttgatttgg
    12121 gcacttatgc tgaatcgccc gctacctttg tccccagtac agattttatg ggtcaatatg
    12181 gtaacgacaa tcactttatc ttatgcacta gggttcgagt cgccagatcc agaaatcatg
    12241 aagcgcccac caagagatcc aaagcaagga atcctatctg gttatcattt gtttcggatc
    12301 atctatgtct ccctactgat catggttcct gcctatttat tggctatgca gtttgaaggg
    12361 caaagcttac agcaaaccat tcttctgcaa aatatcgtct tagctcaagc tgtttacatg
    12421 atcaattgca gagagttgtc caagccgtca ttgaaccaag gtcttttgca aaacaaagcg
    12481 ctttttcttt cattaggtat ccttgcttta ttgcaaggga tggttatttt tctaccagtc
    12541 ggccagcagc tgatcggtac agtaagttta acgatgacac aacagttatt gattggagca
    12601 aatgtgattc ttctattctt agttgtagaa atagaaaaag ggatcatgaa caaactgttt
    12661 cgcaaaaaag aaaaaacagc tagagaatac agtcgctagc cgttactata aaaaagtttt
    12721 gaaagtgagt tcagtcaaac gattgagctc atttttcgtt ggaatagaag aaatgtatac
    12781 aatagggata tctagagaat ctagagggaa attggaaatc aagatatctg ttttttcaaa
    12841 agaaatagtg gaaagatcgg ttagttcgat aggtaagaag cagacacgtt ttcctaagta
    12901 atcttttaat tcctgctgca agaacgcctt ccatgaaggt tctccttgga aaataaaata
    12961 gacattgaac aaattcggtt tgatagaaaa aatcgtttgt tggatcagta gagacaagag
    13021 ttggagaaag taatttggtt cattgatttc tatttctttt tttagatcgt tcagtaaatt
    13081 ctctttctgt tgatacaagt gtgcatattg ctgatcatat gtttctaaaa aacgatggta
    13141 ttgacggtcc aatgcttcaa tcaatatcgg cgattcatga tattttaata tgaggagagc
    13201 caagttatca gtcagagatg ttttttttag ttgatcaaga gacagctctg aagctagttc
    13261 ctctacaaac gtttcgagga cctctaaaaa tggagaatga tgcaggcagc gagcggtttc
    13321 ttgtccatag ttattgttgt aattccaact ttccagaaag cagaaagaag caaaagaaag
    13381 ctcagtgtct ttaagataaa taccttccgt taaatacaat tttttcaatg cagagtcata
    13441 cagggaggag agtgtgctgt tggtatggat agaagaatct aatcggcaat tcgcttttat
    13501 acggatcgta ttgataaaga accaacatgt tccgaaaatc tcttctgttt caaccgctag
    13561 ttcagtttga ctgaggtttc ttaaaaattg aaaataatgg gattcatgga tcgaataatc
    13621 ctcgaaaaaa tagttgttat gagtgaaagg aatcagcaat cgatgataaa aagtacgaat
    13681 cgcacgctct tttccaagaa tacaagctgt atatttcgtc aaatcgatct gcaagtcgta
    13741 acttgctaaa cgctgattga ttttagtcac atgtcttttc aatgtcccat aagaaagccc
    13801 atggtcggct agaaaatcag ggacagcaac tttttttgta aaaagcagat ttttcagcag
    13861 ttcgacactg actgattgtt tgaaaaaaat ctcccaaagg tcatttgtta ataaattttg
    13921 ctggttttgc aaactgattc ctgcttgatc gctgatgatg tcccagcctt cgggcagctg
    13981 agagcgaata gattgaaggt cagaaaaaat cgttcgttcg gttgtcccta tgatttcagc
    14041 taattcattt acggttatct ttgtatgatt cagcaattgt tctactaaag aaacatgacg
    14101 tcgaatatct ttttcggtaa tgattttttt cattagtgaa tacatcaatc agactccctt
    14161 ctattctaag aatacggaga aaataaacgg tggtcaagtt tcaactgatt tttcaatcat
    14221 tgtttatttt tctggagcat ttactgatga taagaatttc atcatgaact tgaaaaatga
    14281 taccactaaa atcaaaaaat aggtgggcaa acaataataa acattttaat agtagatggt
    14341 agcaagacca acacaggcgg attgaacatt taagagttga taaaaaagaa aaatatcgaa
    14401 gtatttgatt agcaactact tgtattcatt tttctattct tacgtggtat ctttgaataa
    14461 agcgaatcaa taagaaagag atggaagcag atgctaacga aaaaagaact aatcgatcac
    14521 atcaatgaaa catacaattt gtctccagaa tatattttta aaaaatttcc tgattattgc
    14581 gtattcagac atcaaaaaag caaaaaatgg tttggggtcc tgatgtctgt tccagaagaa
    14641 aaaatctatg atgatgcaag tgaccatagg atcatcaata ttcttgattt gaaggtcgat
    14701 cctgaacttg gcggaatttt gcgaggaaaa gaggggtatt ttccagccta tcacatgaat
    14761 aaagaacatt ggatctctat tgatctacgt aaaatagaaa agctagatga actagcagat
    14821 ttgattgatg gaagttttaa atcaacgcaa tgaatgtcta ggacaataga gaagaggttg
    14881 tgacagaaat gtttagcttc aagaaataag aaggaattta cgaaaattgc ttctcaaatt
    14941 tttgggaaat ttcggcttat tttctaagaa attacttctg gaacaccgtt tgttcgaaaa
    15001 aagagctgcg acaagaattt tgtcccagct cttttttttt tattttccta aataaaattc
    15061 ccgcatcgtt tttgcaatca ttttgatcaa aggatgatct tctgtgaagc tagaagataa
    15121 ataaattgtt cgtgtcaagg gcaaatcttt gatttcccgc aagacgaaag gatcattttt
    15181 atcgtaagac caagaaactt ttgggaaaaa gctaacagct aatccttgct tgacaataga
    15241 acgtaatgtc gcaggatcat ctgtcgtcga actgatcgtc aattcgatac ttttcgcttc
    15301 aagtgctgca tccagtgtat gacggaaggc gttgctcttt gacagcatca agatgggggt
    15361 gcgttggata tcttctaatt gcagatgttc ttttttagca agaggatgtt tcttaggaat
    15421 ggcgacataa acaggttctt tcatgatagg aatattggtc atgccaggct tcatctccga
    15481 agtgatgtac agtacaggat cggtaatatc atctggtaaa tgatgttgca tgatcgttaa
    15541 ttggatatca ggatgctttt ccataaaaat tcggaccaag ttcggaatag aaacagaggc
    15601 tacctctaaa tacaatatga cggaagtaac gatcttctca ttgaccgaag acaataattt
    15661 gattccttct tctaatgtgt ctaatgtctt gactgcatat ggataaaatt cttctgcata
    15721 aacggtcgtc tcaatgtttc tgccgctatg gtaaaacaaa gggacaccta attcgttttc
    15781 caaatgtttg atcgcacggc tcaaggcagg ttgagaaata aataattctt tggctgcttg
    15841 ggtaacattt tttgtttcga atactttttt aaaatactgc agatgaagca atttcataat
    15901 tatccatccc atccatacct ttttgttatg gttttttctt tttttggcat tttttattat
    15961 agcagaaatt gactaagata gcagtggaaa aagtaagtgc tgaggaggcg aggctcatga
    16021 gtacaggttt cttatttcct ggacaaggca gtcagaagtt agggatgtta gcggatatcc
    16081 ctgaaatgta catggaacgt ttagaaaatg tgacggggta tcggttagtt gataaagaag
    16141 aaaactatca agatacggtt tttatccagc tagcattact gacaaaagca gtattttatt
    16201 tagatgaaat ggaaaaaaac ggtattcagc ctgatattgc agcaggacat tcgattggtg
    16261 ctttttgtgc agctgttgct tgtcgatctt tgagatttga gaatgcagcg gaactagtct
    16321 atcaccgcgc agtcttaatg aaagaagctt atccaagcgg ttatgcaatg ggcgtagtag
    16381 taggtgtgac gagaagcgaa gctgaggaaa ttgtagcaaa aacatttgat cctgaccatc
    16441 cagtttatct gtcaaatgaa aattgtccga tgcaacatac gttatctggg agtatcgaag
    16501 gtttatccgt tacgttggca gaagccaaac ggcaccaagc gcaaacagcg aagctgctga
    16561 aagtccctgt gccatctcac tgtctcttga tgaatgatac ggtcaagaaa ttcaagccgt
    16621 atatgcagtc tgtccatcta tctgatgcta gatgtttgta tataaaaaat acggatggaa
    16681 gagccactca ggacgcagaa gagattcgta aagatttgtt ggaaaaccta gctttccctg
    16741 tgcagtggaa ccaaatgatg gacttggcaa aagaattagg aatggatctg acgattgaat
    16801 ttccccccgg caatactttg acaaagctga ttcatacaaa atttggtgta gataaaggga
    16861 tccgtacgat caatctagac caacatggta ttgatgacgc cacgtttcta tatcaaaaat
    16921 ggagatgact aattatggaa aaaccaaacc gcaactgggc ttctaaacgc gaaaaaaaag
    16981 cgcagcgatt ggaaaaaatc agcaacttgc tcaatggcaa gtatgtagaa actgaaaaaa
    17041 tcgtagaagt aatggaaaaa ctgattgctc ccggcgacaa agttgtatta gaaggcgaca
    17101 atcaaaaaca agcaagtttt ctttcagaaa gtcttgaaaa agtagatgca gaaaaagtga
    17161 atgatctgca tttgatcatg tctagtatct cacgaccaga acatttgaat atttttgaaa
    17221 aaggaatcgc aagcaagatc gatttctctt atgctggtgc acaaagcttg cgtgtcgccc
    17281 aaatgattga agacggcaca atgaaattag gtgatatcca tacttatttg gaactttatg
    17341 gacgcttatt catcgatttg attccaaatg ttgttttagt agctgcagat aaagcagata
    17401 aagacggcaa cttgtacaca ggtttcaata cggaagaaac accaacaatc atcgaagctg
    17461 cagctttcaa agatggtatc gtgatcgttc aagttaacga agttgtcgat tctttaccac
    17521 gtgtagatat ccctggtgac tgggtagacg ttgtcgtgaa agcagataaa ccttatcaat
    17581 tagaagcttt attcacacga gatcctcaaa acatcacaga attgcaaatt ctaatggcaa
    17641 tgatggcaat caaaggaatc tacgcagaac acggcgtaca atctttgaat cacggtattg
    17701 gattcaatac agcagctatc gaattgttac tgccaactta tggtgaatca ctagggttaa
    17761 aagggaaaat cgcgaagaac tgggtattga acccacatcc aacgatgatt ccagccatcg
    17821 aatcaggttg ggttgaaagc atccatagtt ttggtggcga agttggtatg gaaaaatata
    17881 ttgctgctcg cccagatgtc ttttttgtcg gtaaagacgg cacgatgcgc agtaaccgaa
    17941 cattatcaca agctgcagga caatatgcca tcgatatgtt tatcggttca accttgcaat
    18001 tagattacga aggaaactct tctaccgtaa caaaaggacg tttatctggt tttggcggtg
    18061 cgcctaacat gggacataat cctggcggtc gtcgtcattc tacaccagca tggcaatcac
    18121 ttcgcgacaa tgatgatcca ttagggaaag gccaaaaatt agttgttcaa atggttgaaa
    18181 catatggttc aaataaaaaa ccagtctttg tcgaagaatt agatgcacta gctgttcaaa
    18241 aacaagcagg cttagccaat gcaccagtga tgatttatag cgaagatact acacatatcg
    18301 tgacagaaga aggaattgct tatttgtaca aagcaaaaac aaaagaagaa cgacaagcag
    18361 caattgcagc aattggcggt gttacacctc ttggaatgac tagcacgaaa gaatcactag
    18421 atagcttgcg tgcagccggc atcgtgaaat taccagaaga tctaggcatc aaacgtagtg
    18481 aagcgaaacg ttcattatta gctgcacaaa ccatcgatga tttgatcgaa tggtcagatg
    18541 gcctgtatga accaccaatg aaattcagaa cttggtcata aaattggcga gaaaaacctt
    18601 ttgtcgccat ttacccagat ggaagaagaa agacgaataa gcagaacgtg tatgctctgt
    18661 tttacaaaaa ggggagagat ccatggaaca attagcttat aaatatccaa cgaaacagcc
    18721 gatcaataag aaaatccatg taggagtggt cggttcagga gatttggaaa tcttgatgtt
    18781 tccaacagaa aaaacagaat ctactgtaaa aatttgtaca ggaagcgatg gcttcggcga
    18841 agtatggaac aatgtattaa cgagattttt tgatcgttat ccaatttcag cagatatcgt
    18901 gatcaatgac ttcggtgcta caccaggagt tgtgtaccta cgattgacgc aagcaatgga
    18961 ggaactttca gatgaaaaat agttttgtag aattaaacgc cagagaacga gtgaacgctc
    19021 tacttgacaa tggacgtgaa ttgattgatc cattcgatca aatcatcgcg ccaagtttac
    19081 cagcacaagg aatcgtccct gaaagtgatg acgggatcgt tgtcagccgt ggcacgatca
    19141 atggcaaaca agcagtagta atcgctatgg aaggaaaatt ccaaggtggc ggtatcggtg
    19201 aagtttccgg tgcaaaaatc gttgctgcac ttgaacatgt gttagaagaa aataaaaaag
    19261 gaaatgagat ctttccagtc attgtactag atacaggtgg tgttcgtttg caagaagcaa
    19321 actacggact gttatctatt tcagaaatcg gaaatgtaat cattgctcta aaacaatatg
    19381 taccagtcat cggtttgatt ccaggacgtg taggatcatt tggcggtatg tcgattactt
    19441 cagcattaat gagttattta atcgcaacaa aaaaagcgag agtcggtttg aatggaccag
    19501 aagttattga acaagaagca ggcgttcgag aatttgattc tagtgacaaa gacttgattt
    19561 ggaatacatt aggatctcgt caacgggtag aatcaaaaat cattgatgaa ttagtgactg
    19621 atagtgtaga agcaatcaaa gctgctgtca tcgcagcaat ggacgaacga aaagacacgc
    19681 atcgcagtga aagctcagca ttttatttat cattacttga ttctttagat ctttcaaaac
    19741 caatggccat tgacgattac aatgaagcaa ttgctaacca tcaagcaaaa tcaattgact
    19801 taccagaagt agcagaagga aatgaaccag ctgcagcaag tgttggttac gattggtttg
    19861 cggcactaac tggcatgaaa aatccaactt cttctattag tactgtgtat tatggtaaat
    19921 caagtaaatt catagaagaa gccatcgtga catcaatcgt acctgatcct aaaaacccga
    19981 tgtaccgtgt acgaaacggt gaagttggtt tagttgaagg tttccgcatg ggacaaattt
    20041 tggatcacgt ttacgaagaa gataaagaca aaaaagtgaa acggccaatc atcgttgtga
    20101 tcgatgtacc aagccaagca tacggctata acgaagaatt gatcggtatc catgtggctt
    20161 tggctaatag tgctgctgct tatgcaaaac tccgtcaagc tggacatcca gttattggat
    20221 tgattgcagg aaatgctatt tcaggtgcat ttttagcaca tggattacaa tcaagccgct
    20281 tgattgcttt aaacaataaa gaaattaccg tccaagcgat gtctaaagaa agtgctgcac
    20341 gtgtgacaaa acgttcggtt gctgaaatcg aagcagctgc tgaaaaagtt ccagctatcg
    20401 cttacgatat caataattat acaaaactag gagcgatcta tcatttccta gaagatatca
    20461 ccgaccaagc tgcttcagaa caaaatgtca gcaaagtcat cgaagcaatc aatgaagcca
    20521 ttcaagatgt ccgcaataca aatgacacga tgctgacaaa tcgatacaca aacgaaacag
    20581 ccaaaacatt cggacgtgtc gaaacaaata aagtccgtgc caaaatggac gaagaatggg
    20641 atgcataaaa aatgattcaa gcacatgata ttgtcctttt tgataaacat gaaatagaag
    20701 ggttcacctt gcctgaatgg gtcgttctaa ctgaaaagat gttcggaaca gttcgtcgtg
    20761 cgtcgacgga tcacgcagaa atactaccga ttggtttacg tggacctgcc cgcaatcaga
    20821 gatggtcaac agaaatctca aaacatgccg tcaaacaagt cattcatccg tgggacatca
    20881 tcgaacaaga atcgtataga caagagaaaa tcatggatta tcctgtttat cagcaatttc
    20941 atgctgctag aaacatatta tcaggatgta aatggggaat cggcggcagc ttaggatttg
    21001 aattatcgac tgggattccg gcagtaaaag aaaccagtga ttttgatttg ctgctgtacg
    21061 caaattcacc gattgaattg ccaattcagg taattcaatc gcatcctgct ttttttgagc
    21121 aattcgatac ccaagtagtg actcggcaag gcggtttttc actaaaagag tatttgagag
    21181 agccagagaa aaaaattcta ttgaaaacag tcgaaggacc taaattaacc aaagaacttt
    21241 ggtaataaag aaaaagagag agaaagagat gtttgaaaac aaaaaaatca aaatcaacgt
    21301 aagtgacttc cagaaagtat cagctgtaac ggctgttatg atgcaaacga tattgagctt
    21361 tgctttggca aacagccaca gcgcaacaaa tacagctttt cttggtacct tttacgtcat
    21421 cgttaaatac acagcaccaa tgtttatttt tgcaattgtt tataatatgg taaaaacaag
    21481 tcagcatttg tcttattggg aatttttgaa agaaaagttt tttgaactag tcgttccgta
    21541 tcttctatgg acgacggctt atctttggct atttccagca gtccaacaac aaactccgta
    21601 tacggatact gcaagctttt tactgaaata tgtcacaggt gatggagcgc ctcatttatg
    21661 gtataccgtt atgatgctgc agatccaatt gttcatgccg ttttttgttt ggctcggtta
    21721 caaagtattt gctaataaaa aatatgtatg gccagtattg attggttcaa cgattttata
    21781 tgtcgcatgg tatctgttct atcaaacaca agtattgact gggccttatc atgacagctg
    21841 gtacttatta gatcggtttg ttgctagttt catgatttat ggggtagccg catttgttta
    21901 tcacgaaaaa gtctatcaat atttggacag agtacgttat cttttcttac ctgttgcctt
    21961 agttatcgcg ttcttttctg tccgttcatt gctcgcacat ccaggagact tgagctttgc
    22021 gaatgcacct tacttgaata cgatccaaag tttatacagt ttagtcatca tctttgctgt
    22081 cttcataggc gcttccaaaa tgattgtaaa cgattcgcca aaattgccat tgttcaaatg
    22141 gttgtctgtt tatgcttatc gtacgtattt agccaatgta tttgttttcc aagtactgtt
    22201 attattgttt aaagatagtt ggctgcagtt accttcagga gtcatgattc ttgtagctta
    22261 tctaatgacg gcaagttgtg catttgcttt aagctggtta cttcatatca tttgggtagc
    22321 tatcaaaaaa ggtttcagca agtaatagta ggaagaagga gtgagtgtca tgataaatga
    22381 cgaacgaaaa aaagcagtga ctcagcaact cgctgcgttg gtagaggaag cattgatcgc
    22441 agaagtaaca ttaagtccaa aacctggatt agtggatgca ctggatgcgg gtgcacattc
    22501 cgatatggac tatgctttat ttcttaagtc tgctaagacg ctaactcctt tttttgaaga
    22561 aatggctcaa atagcatggc atcatgctat tgatcaggag ttaagagaaa gaattgctga
    22621 gattgggcga aaagcggaaa aggcaatgct gattgctaca aatggcatca atacccataa
    22681 aggagcgatt tgggcaatgg ggctattgat cagtgtcatt gcacaaaaaa tcagtatgga
    22741 acagcctttt tctttatcga aaatacttga tgatgtcagc aagcttgctg cttttcctga
    22801 tacaaaatat cgtcataaaa aaaccactca tggtcatagg gtgaagcaaa aatatggcgt
    22861 aaatggtgca tatgaagaag cgatattagg gtatccgcat atcaggcttg caattcaagc
    22921 agctaaggag ctagataatg attcatatgt acagaaacaa atgcatatgt tgttagtttt
    22981 gatgagttcg ctagatgaca catgcgtatt gcatcgcagt gatcagaaaa cgttagtgga
    23041 aatgcaaaaa cttgctcatg ctgctagtca aagtgcttta ccaaatcgtc catttacagc
    23101 actgttgcac ttttgtcagg aaaaccaaat ctctcctgga ggaagcgcag atctcttgtc
    23161 tgcaagcttg ttcatgctga agatagagga attatggact gccgacacat acatttgtcc
    23221 gatgttactc aatgactaaa taagacaaaa ttgctaaaaa aacagtttat gcatctggtt
    23281 attgactctt ccttttagca aggctatcat aaaaatagtt ttttgtaata aaagataacg
    23341 aggaagacaa tgaaaaaaat caattattta gcaatcattt tgttagtggg cctatgtttg
    23401 tttgctctgc cgacccaagg attcgctgtc gcgggtggtc atggtggagg tacatctagc
    23461 ggtcgaagtt cgggcagagg ttcatttggt ggtcgttcat atgggggcag ttattttggc
    23521 agtcgttctt acggcagaga ttcttatggg tattatggtc caggtacagg tccttctagt
    23581 tatgagcaaa cgggatttct tgcattagct gttttgtcag taggaggggt ttctgctttt
    23641 tctatcgtca agaataaaag acaaaagaaa aggctgaaag cggagatatt gtcccgcatg
    23701 tcaggaacgg ctaaagaaaa gaaagcacag tttctggaaa tccaaaagat attcatgaag
    23761 atccaatccg cttgggaaga agaaactcct gaaaaagcca gaagctgtta tacagacaag
    23821 ctgtttgccg aacatcagaa agttctgcaa aaaaacaaag cagacggtgt aagaaatcat
    23881 actaaaaaaa taactattca ggaagtagga aattatcgac agatccacga aaatagtttt
    23941 gccatccgaa ttgatttcag atgctgtgat tattcagtcg atcgaaaaga tgggcgattg
    24001 atctcaggaa gtaagacaca acgacagtat ttttctcaga tatggtattt tgatttttct
    24061 gagaaagaaa aaagatggca agccgatttt attcagccga ttatgctgga ttgacaggaa
    24121 atatttatga acggagttgt gacaaaagcc ttgtcgcagc tcctttttct gaataaacgg
    24181 tgtgccagag atagcctatc ggcaataagg cacgattttg taaaatatgg gaagcgattt
    24241 ttcaaaatcg cttcttatcg ctcaaggctg aacatctctg tcacaacctc tccactaaat
    24301 ggtgtttaaa agcttgcatc tgcgataata agaacaacca aacgaaaaat aggaagcact
    24361 attttagttt agttgttctt attacttgat gcaggacagc tttttcacaa cctctttttt
    24421 gtatagatgg gcaaaatttt tgtaataata ggcgtatgtc tttttcgttg aggagatgaa
    24481 aaagcatatg gcttattttt tgtttgcttt gcttactttc tttccacttc aagtggcaga
    24541 aatatctact ttttattatg taagcggaac catatacttg aaatcagaag gagatcattt
    24601 ctatgaaaac aagaacaatt gaaatacttc acagaatcat gacagcccaa tatccagtaa
    24661 aagcaaaaca tctagcaagt gagttcaatg tcagtcagcg gacgatccgt caggaagttc
    24721 ttgaagcgaa tatctggttg agcagtaaaa agcttccgga gatccgtaca attcgcaaca
    24781 aaggtttttt gttgaagctg gaaaaggaaa agcaagctcg attagaaact gaaataaaag
    24841 atgtgaaaga cggctttttg aatcggcgcg aacgtatttt tgatctagtt ttagccgttg
    24901 cttacagccg aatcccgatc catctaaaca aaaaagaaga agaatttcaa atctctaaaa
    24961 gtacattaga tgaagatatg agacggttac gagcaacact ggtcagttat gggatagaga
    25021 tcgtcagtta tggaaagcaa ggactggttt ataaaggttc agaacgatct atccgtacga
    25081 tgatctatga ttttatcaat cagaatctgg gaatgaccga cttctttatg aatcaagatg
    25141 cacaattaac accggctcaa aaaatctttc tccattattt tcctttggtg gaaatcaaaa
    25201 aaatacagga gatctattcc aataagttgt tgaaacaaga agatgatatt tataaaaaac
    25261 aaatcctgct ttttacattg atctggatcc agcgaatcag gaaacatgaa tttatctctg
    25321 ccattaattg gaaaaataca gaaataaaag acagtagctt ttctgcattt attgaccaca
    25381 tgatcgagga atttcaattg gacaatgtcc cgcaagtaga aagaaattat cttcgtttca
    25441 ctattgagac gttcaatgct agagatatct ccaattcaat cgagtgggta caagctcaat
    25501 tactaacgat ccaattgatc caatttgtag agaacgagac acacgtacct ttccatctaa
    25561 aggaagaagc tttattcgaa aatctctata agcacatggc tgcactgatt gtaagaatca
    25621 agaatcatat ccaagtcatg aatccattga aagaaaacat ccgaacaaat tatttcccaa
    25681 tctaccgcgc agtcactcat tttgttccta cgatcgaaga agtaattggt ggcaagatac
    25741 tggaggacga gatagctttt cttgtcatcc acttttcgac gatcgccagt gtaatcaagc
    25801 gagacttgaa ttatgtttat aaatcagtcg ttgtctgtaa ccatgggatg gctactggaa
    25861 atctgttagc agagaatctg aaagaaaagt ttcctcagat cgagattact gcagttttaa
    25921 gttcaaaaga aatcgaatta gtagataaat tggatgtcaa tctggttttc agcacctttc
    25981 aattgaatta tcgaaataag ccgcttttag ttgtcgatcc gattttaact gagacgaata
    26041 tcttggttat agaagacttt ttgaaaagaa atgaagaact acagcggtta gagcctaaca
    26101 gtcatgaatc gacggagtta ttcactcaaa taatagatgt catgaaagaa agtggtggcc
    26161 gagtagatcg aatcatctat ggcaagttag aaaaattatt cgaaacaaac aatttagaaa
    26221 tcaacaaaag ggagatccag cctatgttaa aagatatttt gacggacagc aatatcatga
    26281 tcaaagaaaa agccactact tgggaggaat cgattgaact agctgctaga ccattggtta
    26341 aagaaaacat tgtcgaagag cgctatgtcg aagcgatgat tcgtgcagtg aatgattacg
    26401 gaccgtatat cgtgattggc aaacacatgg cactagccca tgcccgacca gaagatgggg
    26461 tcaataaatt aggagtcagc gtagctacga ttgagcaacc aatcgatttc ggtaatcctg
    26521 aaatggatcc agtaaagatt attttttgtt tggcagcagt agactcctac tcgcatctga
    26581 acatcatgaa agaactaatt gaattgatca acaacgaaga gaagttggat cgattgattg
    26641 aatgcacaaa tatcgaagat ttcaaacaac tgcttttttc agtagttgag aaataataaa
    26701 tgatacgggg gagaacaaag atgaacaaat tgaacatttt attcgtatgc ggtgcaggtt
    26761 taggaagtag ttttgcagca cagatgtctg ctgaggatgt tttgaacaaa ttaggagtag
    26821 aagccaaact ggaccattgt gatatttctt ccgcagtttc catgaaacca gatgtgatca
    26881 tcacagcaga aaattttcgt tcgcagttcg aaaagttcgc agttgatcct aaaacggcga
    26941 tgatctattt gaaaaatatt gtttcaaaaa atgaaattga agaaaaaatc accccagttc
    27001 tgcaagataa gggagttatc taaatacaaa aagaaaagag ggtaaaaa
//
LOCUS       DACECR010000031        26297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.031, whole genome shotgun sequence.
ACCESSION   DACECR010000031 DACECR010000000
VERSION     DACECR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.031"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(72..1274)
                     /locus_tag="IWO17_001222"
     CDS             complement(72..1274)
                     /locus_tag="IWO17_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0290110.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1391..1906
                     /locus_tag="IWO17_001223"
     CDS             1391..1906
                     /locus_tag="IWO17_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0290111.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2105..2551)
                     /locus_tag="IWO17_001224"
     CDS             complement(2105..2551)
                     /locus_tag="IWO17_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0290112.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2598..4076)
                     /gene="msr"
                     /locus_tag="IWO17_001225"
     CDS             complement(2598..4076)
                     /gene="msr"
                     /locus_tag="IWO17_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0290113.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4430..5059)
                     /locus_tag="IWO17_001226"
     CDS             complement(4430..5059)
                     /locus_tag="IWO17_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0290114.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(5043..5507)
                     /locus_tag="IWO17_001227"
     CDS             complement(5043..5507)
                     /locus_tag="IWO17_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0290115.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5994..7262)
                     /locus_tag="IWO17_001228"
     CDS             complement(5994..7262)
                     /locus_tag="IWO17_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0290116.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7493..8230)
                     /locus_tag="IWO17_001229"
     CDS             complement(7493..8230)
                     /locus_tag="IWO17_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0290117.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8297..8865)
                     /locus_tag="IWO17_001230"
                     /pseudo
     CDS             complement(8297..8865)
                     /locus_tag="IWO17_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            8993..9865
                     /locus_tag="IWO17_001231"
     CDS             8993..9865
                     /locus_tag="IWO17_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0290118.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10207..11079
                     /locus_tag="IWO17_001232"
     CDS             10207..11079
                     /locus_tag="IWO17_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0290119.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11076..13034
                     /locus_tag="IWO17_001233"
     CDS             11076..13034
                     /locus_tag="IWO17_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0290120.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13366..14217)
                     /locus_tag="IWO17_001234"
     CDS             complement(13366..14217)
                     /locus_tag="IWO17_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0290121.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14340..14807
                     /locus_tag="IWO17_001235"
     CDS             14340..14807
                     /locus_tag="IWO17_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0290122.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(15070..15552)
                     /locus_tag="IWO17_001236"
     CDS             complement(15070..15552)
                     /locus_tag="IWO17_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0290123.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15652..16515)
                     /locus_tag="IWO17_001237"
     CDS             complement(15652..16515)
                     /locus_tag="IWO17_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0290124.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            17113..17988
                     /locus_tag="IWO17_001238"
     CDS             17113..17988
                     /locus_tag="IWO17_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290125.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(18133..18285)
                     /locus_tag="IWO17_001239"
                     /pseudo
     CDS             complement(18133..18285)
                     /locus_tag="IWO17_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(18553..19884)
                     /locus_tag="IWO17_001240"
     CDS             complement(18553..19884)
                     /locus_tag="IWO17_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0290126.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(20033..20746)
                     /locus_tag="IWO17_001241"
     CDS             complement(20033..20746)
                     /locus_tag="IWO17_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0290127.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            20921..22345
                     /locus_tag="IWO17_001242"
     CDS             20921..22345
                     /locus_tag="IWO17_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0290128.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            22644..23351
                     /locus_tag="IWO17_001243"
     CDS             22644..23351
                     /locus_tag="IWO17_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ0290129.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            23338..23661
                     /locus_tag="IWO17_001244"
     CDS             23338..23661
                     /locus_tag="IWO17_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0290130.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(23750..24787)
                     /locus_tag="IWO17_001245"
     CDS             complement(23750..24787)
                     /locus_tag="IWO17_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0290131.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(25108..26091)
                     /locus_tag="IWO17_001246"
     CDS             complement(25108..26091)
                     /locus_tag="IWO17_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ0290132.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
BASE COUNT         7987 a         5300 c         4610 g         8400 t
ORIGIN      
        1 atgagaagct atttttgttt ggctgttctt atgacttgat gcaggacagc tttttcacaa
       61 cctctgattt cttattcttc ttcatttgta gcaaaataat tacggtgata aatgatacaa
      121 catacactga taagaacaat ggcacctgca aaataaaacg catagctttc accaatatgt
      181 tcaccaatca cacctgcaat aaatggtgct aaagctccac cgaaaaagcg gacaaaacta
      241 tagctggacg aagcaacgtt tcgttgcaga cctggtattt ccatagcaat cgtcgtcaac
      301 agtgtattga taattccttg gaaaaatcct gctaacacaa tgctgatcgg tacaaggact
      361 ggcatagaag ttccagctcc taataacagc aaacaaataa tgaataagac aaatgctgct
      421 aaaattgtcc gataagtcgt aaacttccgc tcaagagccg gtgcaacgaa aatcgaagca
      481 atagccagaa gcacacccca gccaaaaaat acaaaaccga cttctaattc agaatagcca
      541 atcaataaaa atggcgcata cgcaagtaaa gtaaagaatc cgaaattata taacaatgcg
      601 attagtccaa ctgaacgaag tttatgattg cttaacgctt tgacaccttc ccaaaggcct
      661 acttttctct ttggtttctc agtttcaggt aaaagtaccg ctagggctat aaaaccgcaa
      721 aacatcaaaa atgctacccc aaaaaatgga taacgccatg aaccactgcc caagacccca
      781 ccgactagag ggccaacagc cattccgcag ccaagtgctg cttcatacat catgattgct
      841 ttttctgtgt taccaactaa aatactgacg atcgttgcta aagcagtaga gacaaataaa
      901 gcattcccga caccccagcc ggcacgtaac ccaatcagca ttccgatatt cccagataat
      961 ccgccaagac cagcgaatac aacgatgata aacaatcctg tcatcaatgt ttttttcaaa
     1021 ccgatccgac tagataagaa acctgtaaat agcatcacca caccagtgac taacatgtaa
     1081 ctagtaaata acagggtcgt ttcagctggc gtagcgttta actgtgcagc aatcgttttt
     1141 aagattgggt ctactagacc cactcccata aaagcgatca tacaggtagc tgctacaact
     1201 agcgctgaac ttttcccctt tgtttcttca tttgtttgtt cttgtatatc tatttgattc
     1261 tccaatattt ccatcccttt ctctctatta gcatcataca tgatttattt tacatgtcaa
     1321 tatgcacatg taaaacgtgg aaactttact gtgatataat aaagaaaata agacgagagg
     1381 taggattttt atgaatactt tagcctatat attattatgc gtgcttttat ccaaaccacg
     1441 ttctgggtat gaattgaagc agctgatcac aattttttgg gaagcccatc acagtcagat
     1501 ctatacaacg cttgccaagt tagaccagca gggttatgtg accgttttgg acaaagacga
     1561 gcactctcaa aaaaagatct accacttgac tgatgaagga cgtgctcttg tagaggaatg
     1621 gatcaaagag gaaactcccg ctcctacgca aaaagatgaa tttctagcaa aaatctatgc
     1681 tttttcgacg ctcgataaaa caacagctac tggactgcta ttcactagag aacaacagct
     1741 gaacaaaatc atgcaaaaaa atcaaaaaaa attgaatgac ctctcgctag acaagaaaca
     1801 agaatttgga cgttatgttg tgatccagcg cagaattatg ctttgtcagc aggaactcca
     1861 atggtgtcag caagtaagag aacaaatgca gcagtttttt gagtaaggga taaacagaac
     1921 ctttctagaa atatctattg aagagcaaat tatcttcaat aaaagagcaa tacagttgga
     1981 aaagaaaaag cgatccgaac gacaggccaa aacctatcgt tcggatcact atttttctag
     2041 aactgcattc aagagactat gacaaaaagc atgccaaggc cttttttcaa ataacagtgt
     2101 tccatcattc attactttga attgctttta gcacacttct tgaaagcggg ccaacgatga
     2161 tcaactgcaa cggtaaagcg gcaatcacat tcattttcca agcattcata taagcagaga
     2221 ataattgatc aggtattcct tgttccatga tcacaccgaa taaagacata catgtcacca
     2281 ttcctaaaac catcaagcat gaaatagtta agatcatttg gaccttgctt tctggattta
     2341 ctggtagttt aaaggcaatt tttttcgcca gcacaccgat gatgaagaca tccaaaatga
     2401 aagccaccac gaatccaggc actagaccaa tcaacaaact tgataaatga agatcttgat
     2461 gcagccataa attatacgcg ctcatcccca agaccatcaa aaaacacatc attgttgtaa
     2521 aaattattcc ttctttttta tttgtaggca atctgtacca ctcctttaaa tttgtcacga
     2581 aaatcatcgt agcacattta aaaatttctc gtaagtactt tttttatttt ctgccctttt
     2641 atttcataaa cttcatcagc cgtacgctcg acaaagtaag aatcatgaga agtaaacaaa
     2701 atcgttcccg gatatatttg cattagcttc tctaaagctt cgatcgttgc catatcaata
     2761 aaattagtcg gttcatctaa cagcaacaca ttacttggct tagtaaaaag caaagcaatc
     2821 gtcagacggg tcgcttctcc cccacttaat gtacataacg gtttcgtcaa agtttcctga
     2881 gcaaacccta agtgattcag caaactacgg acaagcgatt ctgaaaactc tgtttgtctc
     2941 atgacaaaac gaatgatgga ttcttcagat gtcatttgat aacccttttg ctgatatact
     3001 tgaaagctta ctttcggaga gagaaggatt ccttctcctt gtttgcggat atgttctaat
     3061 aaggttgtct tacctgaacc attttcaccg acaatcgcga tttttttacc taaagaaaac
     3121 tgaaaatcac catttaccag taatgttctc ccacttcttt ccaattggac attttgtccc
     3181 atgatgggat agcggctgtg gagttcaaga gattttggta ctggaaaacg gatttgtttc
     3241 atctgctgcg gcttggttgt ttctcctatt ttttggagtc ttctctctaa tgttttcgca
     3301 ttcttttgga tggctttttg tacactgtct ttttgtttag aggaagacaa ccgatccagt
     3361 ctgatttgtt gctttttctt tttactggac actttttcca acttttccgc ttgttttttc
     3421 ttttcttgga tcgatttttc caatcgtttt ttctctttca tgatcgactc tgcttctcgt
     3481 agctgagtct ttttctccaa ttccttttga gtgagatagg cactataatt ccccgaaaac
     3541 tctcggattt ctccgtcttt tacttcccag attttcgatg ctagctcatc tagaaaatag
     3601 cgatcatgac taacaaaaac cagcgtacca taataataac gaagctcttc aatcagtcga
     3661 tcaatacttt ttctatctag atgggttgtt ggctcgtcta gaagcatccc ttgttcataa
     3721 gaagaaagaa gacgtatcaa ctcgatcttt cgttcttccc ctccgctcca tttccgctct
     3781 gaagtttctt ccagtcttaa ctggaaatga ctggctaatt ccaaatttag gtcctttgct
     3841 tcagcgagat aggttaattg aggcaggtag ttgaaggtga tctctgtttg gattttccca
     3901 tgatcgggaa aaagttcacc ggctattagt ttgagcaaag tcgatttacc gacaccattt
     3961 tctcctatga tccctatacg atcttgctga tagacacgaa gagaatcaat agatagttct
     4021 agctggttgc caaagctgac ttgtagattt gttatattta ctgctaaatt ttccatatgt
     4081 tttacactcc tatttcatgt aaaacaacaa ttatcgatac taaaaaagag cagaaaaatc
     4141 tgtttgcaaa aaaactgcat cacatctttt cctactctga acgaaataac tatgactaaa
     4201 tcaccaaatc tgtcatagct ttcacctatt ctactgtcgt tcgaaaaaat ggacatactc
     4261 catccatgaa attgttgttt atacatgtct tttgaattta tttagttgtt attcaaaagt
     4321 tcgaaacgta atttcatcga tgcagtcatt tttccgacct ccttttttat taggttacca
     4381 cacacgattc aaaaaaagca atagctactg aatagtaact gaaaaccttt tattcgaaca
     4441 tagtgatctt ttctggtgct aaacgaacag actcgtaacc ttcttctacc gcttttccta
     4501 ttgataaaat aataactgga acataacggt ctttatccaa accaaatgcc tctgccaact
     4561 gatctgcttc gaatccacca attggatttg tttcatagcc atgatcacgc gcaactaaca
     4621 tgaattgcat agccgctaag ctagaatcga ctttgactac atcgttcatt tcttctcttg
     4681 aaaaactctt gtaataagga atgatcgcag ctaattgttg atctctgact tcttttggca
     4741 tcttccctga ttcgtaagct tggttgtaga tttcttcgcc atattcgtaa cattgcatat
     4801 cgccaaaaat catgaccatt gcagaagaag tatcattttg tctcgtattg aaacggatca
     4861 aaggacgcaa gatttctttt gcttcctcgc tttcagcgac cacaaagcgc catggtttca
     4921 tattgacaga agaaggtgca gtcgtcgctt ctttgatcat ttccagcatt tcttcgtgtg
     4981 ggattttgta tgtttcatca tatacgcgaa cggatcttct accagtcata attgttgaaa
     5041 aatcattttt ttgttccatt attattaacc tctttcgttt ctgttgttat attgcaaagt
     5101 tgggtattca atttatctaa aagtgttaga aattgtttca tttctacatc gctgaaagcc
     5161 ggggatagca cggaacctgt atctttatgg tccatttcac aatgcttgat tttcttctgg
     5221 gctttttctg ttagcgctac atacacttcc cggttgttct gaggatttct ctgacgcgaa
     5281 acatagccat ttgcttccaa taccttcaga tgacgagtaa ctgctccttg atcaatctgc
     5341 aacttttctt gcaaactgct ttgtagcaat ggattttctt ctttcagtct catcatgatc
     5401 tcgtatctag taagacttat accgatattt tcttcaaaga tctggacgat tttttgatcg
     5461 gctaatttga tttgatataa aaggtgacta atctcttcta aagacataat ttcctccttt
     5521 ctaataattg actcgtcaac gattgacttg tcaataatag aataaaaaaa gaaaaaagtc
     5581 aaagaatcag ctgtgtaata aaaaaaacgc tctcaataga aaagtataga gaaaagaaat
     5641 tttttgtaga atgaacggaa aaaatgataa agaaagcaca acgctaaaac caagattatt
     5701 gattcttatc aaactataga agatagctct tctgcagtag ataattttac accgccaaaa
     5761 atttccgaaa tcgccttttg caagaatccc gtcgaatatt agaaattgga tcagtatgct
     5821 taaacagtaa taaaaaagtt atgacaagat gttgtcacaa ccttttttga gtaaacggtg
     5881 ttcaaaaact tgcatctgcg gtaataagtt caaccaaaca aaaaacatga aaatctgttt
     5941 ttgtttggct gttcttacta cttgatgcag ggcagctttt tcactacctc tttttaataa
     6001 ccataaaatc ctattccttc atatctccag cccatcggtg ttagccaatc tctttcctta
     6061 gcactcaagg tatagtgatg ggtaccagtt ttttcatttg gattaaatag tcgataaatt
     6121 ggcttggaac caccagaata aaaacttaga ccttcataac gccaaccgat ttttgttaaa
     6181 gagttgatct cagattgtgc cattgtgtaa tgatggtctc cactgtaagg attgtataaa
     6241 cggtagacag gttgtccgct gtttggtgct tgccaagcaa tcccttcata ttcccagcct
     6301 acagttggca gataattttt ttctccggca ttcaatgtat ataaatgttc tcctgtatga
     6361 cggttataca gacgatacat cggagaagtt gaagttggtg ctgtatcata ctgtgtcaga
     6421 ttataggttt cgattacatt gttcaatgta gaaccataat taggcgcagt ggcataacga
     6481 cctgttagcc aagccgtagc atctttgtaa gagttcgtat tgcttttcca tgcaccggaa
     6541 tagtagtaaa cgcctggctg aaaagaagtc gtcttcaata ccgttacgtt atcttgcaat
     6601 gattctttgt acgaaggata ttttctaaaa gcggcattca ccgtaatcca ttgcccattc
     6661 acatattccg aagtaggcat cgtaacagat tggccattat agcttccttt aatcccaaat
     6721 agattgtaat taggggcaga agccaaagta ctgttccccc agccactttc aacgatggct
     6781 tgagcaatca tgactgatgc atataagtca ttggcacttg ccaaactttg tgcagttgag
     6841 gcaatactat caatgaacgc ttgttgagaa ttagcagatg agcgagcata aaaagcatag
     6901 ccagtatagt tttcgaattc agacaaatct gcttgatggg tatgtgtcaa tgtttgccca
     6961 tctgtcaatt ggttcgtgat ttcttctgca ttctctggat tgttctgagc ttctgctgat
     7021 gtactgtctt cggtttgtgt ttgttctgtt gtttcagttg ttggttcttc ggttgaaatt
     7081 gtattttctg agacagtagc tgactgcaaa gtactttctg tatcgttttc aattcctgtt
     7141 tctgaagaac tcgatgtctg agttgcgaca gtttctgtcg attcttcagc tagcactgtt
     7201 gtgttggcaa ctgctagatt tcctaaaaga ataacgctaa ataataaatt ctttgctttc
     7261 atcttttttc tcctttgaat tatgaaactt ttcctaaata acagtgtaac taaaaaagta
     7321 aaagcaagtc aatgtccata tttaaataac atttgtttct ttttaataaa tttgcttttt
     7381 ctgcataaaa agacgtaaat gaagaatatt cttctccatt cacgccttaa attgatggtc
     7441 gtttgaataa ctatttaaca agtaattctc tctggatatt atccgttatg gattatactt
     7501 cctgtgtcgt cggacggata atcatgttgc tgacagacat ccggtctggc gtttctatcg
     7561 caaatgctac agcagaagct atatcttcag atgttaatcc ccattctttt tgcgcttcat
     7621 gaagttcaag agcagccttt ttgtcgctga tggtttgata taattcagtt tgaacagctc
     7681 ctggagagat gatcgtagat ttgatgttat tttgccgttg ctcttgacgt aatccttcca
     7741 tgattgcacg gacagcaaat tttgttccgc aataaacggc ggaatcagga taaacaacat
     7801 gtcccgccac agaatcagtt gaaatgatat gcccagattt ttgtttttcc atgattggaa
     7861 gtacggcgga aattccattt aacacaccca tgatgttgat atcaagcata ttttgccatt
     7921 catcacggtg accttcaact aatggagcgg ttggcatgat tcctgcatta ttgtacaata
     7981 catcgattcg accgaacttt tccatcgcta aatcgataac tgcttggact tgtgcaaaat
     8041 ctcttacatc tgctgtttga atatatagct cagcttctgg taaagattct tttattgctt
     8101 ttaggcgatc ttctcggcgt gcagctataa ctagtttagc ccctttttct gctaatagct
     8161 tcgttgttgc ttcaccgatt ccgcttgaag cgcccatgat gacaatcact ttttctgata
     8221 aatcattcat tctattctcc tcctgttttg aaaaatctct tgcttatact cactatctct
     8281 tatttcatct tactgttcaa ataatccgtt cgacaggttt gacgattttg gcagggattc
     8341 ctgcaacgat ggtattgtct gggacatctt tcgtaacagt gcattcgctg caacaatggc
     8401 attctcgcca atcgttacgc ctggcagaat agttgctcct gctccgatcc aagcattttt
     8461 cttgatgtgg attggcttta ctgacaagcc tcttcgttct tttggatcgg ttagatggtt
     8521 caccgtgatg aggttgacac gaggaccaat caatactgaa tcttctaaac agatgccacc
     8581 taagtcaacg aacatcactt gacgattgat aaagacattt ttccctatac taatatgttt
     8641 gccgaaatct gtataaaaag gcagcagaac agtcaccgta tcatctactt tgtcactaat
     8701 gatattttct agccgtttgc ggatatctgc tggttcatgg tacccagtat tcagcgctgc
     8761 taaaggctct tcgttttccg cttggatctg atggatcact tcaaagagcg gatccgttac
     8821 tcgaatctct ttattgacga tttgtttttc gatgaggctt gtcattttgc tccctccttt
     8881 tatccttact ttataacgga cggcttatga tgtctaatac ttataaaaca tacttaacta
     8941 ttccttttaa ctatacttag tggtatgctt ttttaaaaac aggagggaaa aaatggatat
     9001 tcgtatactg acgtattttt tagcagtagc tagagaaaaa aatatcagcc gagcagcaaa
     9061 atctctgcat atcacgcaac ctactctctc aaaacaactg aaagatttag aagaagaact
     9121 tggtgtgaca cttttcactc gaggctcccg agagatacag ttgacggatt ctggtcgata
     9181 tctttattca aaaggaaaag aaatcttgtc gttggtagaa aaaacggcga acaatttaac
     9241 aaatgacgca atcatctctg gagaaatcta cattggcgga ggcgaaacta aagcaatctc
     9301 tttcatttct gatagccttc attcgttgat caaggcgcat cctgatatcc agtttcattt
     9361 atatagcggg aatgcagatg atatcataga caaactggat agcggattac ttgattttgg
     9421 actcgtaatc gaaccgacaa acaaacaaaa ataccagtca tttcgactgc cagcccaaga
     9481 tcgttgggga cttttgatac atcggaatca tcctttagca aagcaaacag tcatccatcc
     9541 gcaggaactg aaaactgttc ctttgatcat ttcccggcaa acgtcagtgg acagtcaact
     9601 agctgaatgg ctaggtttca gcatagatca gttatcgatc gtcggaacat ataacttatt
     9661 gtacaatgct tctttgatgg tcgataacgg gacttttggt gcattagctc tagatgggat
     9721 catcaatact tatgggacgg aaaaagtatt catcccactc gatccgccat tacaagcttc
     9781 actgaatctc atttggaaaa aagaccagcc tctttctcct gctgctcgta aatttttaga
     9841 tgtcctaacg gagaaataca gttaagtaga gtttttttcc ttcgctttca gctttttaac
     9901 aacgaataag ttaagcaaaa gatccaatga ttaaataaca taagtttctt aatttgttcc
     9961 ttccttttat ttctgataat tccagtaaat tactataatt ataaaaaaag aattgacaaa
    10021 acataactgt ctatataaaa ttgattacgt taacaataat tcgtttcatg tgactagatc
    10081 atactaggca tgaaaaatct ctgaggaaca tggtttccct aggaggtttt tcgtgtctat
    10141 ttttgtttgg ccgcaatcaa ctggtctagc aaacgtgaaa accaaatctt caaaaagggt
    10201 gcgcgcatga aaatcaaaaa gatctttaat caaaatgcag tattggtaga tgacggtgga
    10261 caggaaaaag tagctattgg aaaagggatt ggatttgaca gaaaaagaaa tgatttgatt
    10321 tttgatcgtg atgtcgaacg gatatttgtc atggaaccag aaggtcaaat caagttgcaa
    10381 aatttactta atcagattga tgaacgcttt ttatttgcag cagaaacgat cattgatcat
    10441 gcggaaactg ttttgatgga aaagttgaat gaacatgtat tgatcgcttt gaccgaccac
    10501 attgcttttt ctgcggagaa cattaataac ggtatcgtca tccgcaacaa acttttaaga
    10561 gaaatcgaag tgctttacag tgatgaattc agtattgctc aatgggcagt agagtatctg
    10621 acaaaagagc ttgggattcc atatacttat gacgaagcag gatatatcgc aatccatatt
    10681 cacagcggac gaagcggtcg gaacaataac catcgcagta tccgtgaagt gacgattgta
    10741 tctgacatca tccatttagt ggaatcagaa ttggaaacag atatccattc agaaccattt
    10801 tcactgaatt attcaagact ggtgaatcat ttacgactgc ttctacaacg aacccatgcg
    10861 caacaatatg ctgttttaga cacggaaatc gtagaaatgg tcaaacgaaa atacccagaa
    10921 agctataaga tttctaaaaa aatccgtgtg ttgctgacca aagaatacca gctcgcgatc
    10981 acgaaagaag aactgggtta cttggccatc catatcgagc gtttaagaag cgcaatcgta
    11041 caaacaaatg ttaacaatca tgaggaggaa aaaaattgaa agcatatcta caacgaatgg
    11101 gacgttccct tcaacttcca gttgcggtct tacctgctgc tgctttacta gtagggatcg
    11161 gaaactggtg ggcatcttat agtaacgaca ttgtcgctca ttttttacaa gctggcggta
    11221 acgcagtttt aggtcaatta cctttgctgt ttgccgttgg gttagcacta gggatgtcag
    11281 tggacaaatc aggtgctgct gccttggcag gtttggtcgc atttgaagtt ccagtaaatg
    11341 ttttaaaaac tgattcagta gctactttat tgaatatcaa accagaatca gttgatccat
    11401 cttttgctca gatcagtaac gtatttattg gtattattgc cggattgatt gcggcagctc
    11461 tttataatcg tttccacgaa gcaaaattac cgatggcttt atctttcttt agcggcaagc
    11521 gtctagtgcc aatcttggct gcctttgtca tgttgctgat cagtgcagtg ctactgctcg
    11581 tatggcctcc ggtttactct gctttagttt cttttgctaa attcattttg agtttaggct
    11641 ggggtggtgc aggtctttat ggcttcttca accgtttact gatccctaca ggattgcatc
    11701 aagcaatgaa ctccgtgttc tggtttgacg tagcaggaat caatgatatt ggtaaatttt
    11761 tggcttctga aggaacaaaa ggcgtaacag gaatgtatca agctggattt ttcccaatca
    11821 tgatgttcgg gcttccagca ggagcatatg ctatttacag aaatgcacgt ccagaaaaca
    11881 aagcaaaaac agcttccttg atgttagcag cagcttttgc ttcattcttt actggtgtaa
    11941 cagaaccact tgaattctca tttatgttcg ttgcttggcc attatatgtc ttgcatgcaa
    12001 ttttcactgg gatttcttta gcagtgagtg cattcttcca ttggacagca ggatttgcct
    12061 ttagtgccgg atttgtcgat tacttcctat ctttgaaaaa tccgattgcg aatcagccat
    12121 tgatgttgat cgtccaagga ttagtaacag cagtgattta ctactttggc tttaactttg
    12181 ctatcaaaaa attccatctg atgactcctg gccgcgagga agcagatctt tcagatgacg
    12241 atactgcaac gactaattca gacaataaat atgcggcaca agcaagcaaa atctacgctg
    12301 cattaggcgg agccgacaat gtaacttcga tcgataactg tactacacgc cttcgtttgc
    12361 aagtcaaaga tacaggcctg atcgatcaaa ataaaatcaa agccactggc gtacctggta
    12421 tgaagatcat cgacggaaaa aatgcgcaag tcatcgtagg tacagaagta caattcgttg
    12481 cagatgagat ggcaaaacta catggtggtg ctgcaacacg acctgcacaa acaaacaccg
    12541 tggttaaaac agaaacattt gccggtggaa cgatcgaaca agatatttac gctatcgcaa
    12601 atgggaaact gattccaatc actgaagttt cagatgatgt attcgcggaa aaaatgatgg
    12661 gtgacggcta tgctgtatta ccagaaaatg gagaaatctt ctctccaatc gctggtacga
    12721 tcacaaacat tttcccaacg aaacatgctg tcggaatcca aacagatgct ggtatcgaag
    12781 tcttgcttca tatgggcatc aacactgttg acttaaaagg cgaaccattc actttatatg
    12841 taaaagaagg tcaaaaagtc gcaagaggtc aattgattgc tttagttgat cttgccgcta
    12901 ttcaatctgc aggcaaaaac acggatatgg tcgttgtatt cacaaatggc gacaaagtcc
    12961 aaagcttaga aatcgaaccc gcacgtgatg taaaagcaaa tgataaaatc ggctctgttt
    13021 ccaacaaagc ctgagccatc gtcttcccca gactttttta tcgataaaca aacaggagct
    13081 gagacaaaaa tcttgtctca gctccttttt ccgaataaaa acggcgttct cctgccaact
    13141 ccgcggtaat aagtccagcc aaacgtaaca tatgaagagc tattttttat ttgactcttc
    13201 ttattacttg atgcagaaca gcttttttac aacctctgat aaacggtgtt caaaaaccag
    13261 tttatcggta tgaagtcaaa tttatgcaaa aatgtggaaa gccattttca caaaattttt
    13321 cttcatactc aaaactaatc ggctttttca caacctcttt atacattatt cctgatccaa
    13381 tatttctttt aatgctgctt caagagaagt taatggtttt cccaatactt tttctaaatc
    13441 atcagaggaa atagctaact gatccttttt aatatcatgt tggatcgcta aaaacatgtc
    13501 agctgcttct tgagggaatc cggcttcttt tagattttcg acaaacgctg catcacttgc
    13561 ttcaataatt tctaactttt tacctgtcgc acgctctaat gccttggcta attcttcata
    13621 gtcagttaga ttgccggata attctaagat ttctggagag tcactttcta ctagtacttt
    13681 tgctgcagct tctgcatatt cacgtttcaa tgcccaacct gtttttccat ttcctgcagc
    13741 ataaacgaat tgaccagttt ttaaagcctg cccaatgata ggtaattcat tctctacata
    13801 ccaattattc cgtaaaaatg tatgggaaat acctgaatcc ttaatcattt tttcagtgaa
    13861 ttggtgatct ggcgctaata cactgatcga gtgatcggca tctgcaaaac ttgtataagc
    13921 gatataagat accccagctg cttgtgctgc cttcactaca ttcttatgtt cggcttgacg
    13981 atttcctgga acgccagaga caaaaagcaa tcgatctatt cctttcaacg cttctttcat
    14041 tgagtctaaa tcatcataat caccaatacg tatattaaat ccagcttctt tcagtgcttc
    14101 tcctttttct tcactacgta ctagtacata gatttcattt tgtggaacca tcttttttaa
    14161 gaattccagc gcataaccac caaaacctcc agttgctcct gttactaaat aatgcatatt
    14221 tgccactccc tattcttttt atttgttaag atttacagct gtacatttat aaattacacc
    14281 tttatcataa agagtgtggt aagataagtc aagcaaaaaa aatgaaagga tgacaactga
    14341 tgaagtattc tctgcaattt agtgatgcaa tccatgtact tgcctatatt gaaatgtttc
    14401 aagatacccg atcactttct agtgagatga ttgccagtag tatcgaaaca aatgcagcaa
    14461 atgtacgtaa aatcatgagt cagctcaaaa aatccggttt gatctttaca aaaaccggaa
    14521 aaccgcagcc ttcccttgcc aaaagtccag atcagatttc tttactagat gtctatcgca
    14581 gcatcgaagg taatacacag cttatccaag tcgatccaaa aacaaatcca aattgcgtcg
    14641 ttggtgcgaa tatccaagaa gctttagaag aaagctacgc tcggctgcag aaaaaggcag
    14701 aagaagaaat ggctaagatc acacttgata gtctgataca taaaatagcc gttttggaaa
    14761 aagaaaaaag accagaaaac acaggcctcg tagagagatt tttataattc tctctactgt
    14821 gcctgcattt ttctgatcgg aatcgtctac ataaattctt ttattctccg ggggacaaaa
    14881 gccttgtcat gccccttttt cctctctaat gctcaggtca aagcgctgaa gtcatgacgt
    14941 cttgataaac ggtgttcaag tagctgaccc tcggcattag ttctgaaaaa tgcaaaatat
    15001 ggaaagctat ttcccatttt tcctctctaa tgctcaggtc aaagcgctac tttcacgacc
    15061 tctatattat catggtttat tgactaccgg tatccagatt tctcccatat atttcccgtt
    15121 ggtaactccc atttcgacta cagcatttgg acccccaaca taggcgtaat cttctacttc
    15181 tcttaaaaca tgcccaaagg ccgtattcgt caaatcattt cttaattctt caggcgattc
    15241 tgctaaagcg attacaacaa gatacgttcc ttttgggaat gggatgattc gggtagtgtt
    15301 tggaacatcc tgactcgttt gaacaccgac atagtacatc attttcccgt tatagggttc
    15361 gttgactgta aagaaataat gattttcagc agcttgtttc aatgcatcta ttgttccatt
    15421 accgataact ttttgctcaa atgcttgttt ctctgcagcc aaaccgacaa aatccgtatt
    15481 gctactcttc aattccacac caatacccgt caccgtaaaa tctggttttt cttcgatcct
    15541 atactttgcc atttctccca ccctttctat tatattaagt tcatcttaca taaaattaaa
    15601 gggaaatgac acgattacgc tcttttttct agggatattt ttatttttta actatttttt
    15661 tgctttcact gcaatccaaa tctcactgta aacattactg aaatcctctc cccattttgt
    15721 aaaagagatc tcgggagctt cgactaactc ataatctgaa gaaggaagcc attctgcata
    15781 gattttcccc caagtatctt gtaaagtttg gggaaatggc cctttattag gaaaaatggc
    15841 ccaagtgtgt tcttctatag caagcatttc taaatcagcg tacggatttt cttttgtcgt
    15901 agcaaagcca atcatgtgca tcaaactgcc cttttcttcc aagcgggctc catcaaatcc
    15961 ataagaaacg ttgattactt gatttggata taaatctcct aacttatgca tctcttccct
    16021 ttgagtctcc gtgatcgtct gcgctaactt ttgtatttct tgattgactc cttcaaattt
    16081 gacaggaact tttttcgcca cacctgccac ataaaactgt tctttctttt cgatacgaaa
    16141 ttccatcgat tttcctcctc tgacatcaat aaaaaatgtc aagcgtggaa aagatttttg
    16201 tatgccgtat ctgctgattt ctgaaggcag atatcctgac caatcacgaa aagctcttga
    16261 aaaaccttcg actgtttcat atccatatct aaacgccgtt tctgtcactc ttgatccatc
    16321 taggagatcg cgattagcca aagccaatct acggtttttg atatagtcca ttagcgacat
    16381 ccctgcaata aacgtaaaga tgcgttttaa ttgatattca gagattcctg tattttcggc
    16441 tattatttta taggaaagtc cttctgtcaa atgttcttcg atgtaatcga tcaattggtt
    16501 cagatcttta agcatattta tcttcctcct tgcttgcctt tttatcatat tatcaaagaa
    16561 agtcaattac ttgatgaatc aggaaccaat ttagtcagat gtagaaaatg ttgtatggat
    16621 ctaaagaaaa ttctcaagca gcgtttcgta acggataatt ggtattatct gcctatctat
    16681 gttttacctt tagaaaaata aattttgggg aagacacatt ctaagtaaac aaagcggtaa
    16741 aaatatcttt tattccctta gttaatccgc ttactaccta ctgtacaaag caaattgttg
    16801 ttcaaactgc tgatcagaac gctgcttgga tttttctttg cgatacatca tacctctttt
    16861 atcaattgga acaattattt atttctaaca aaatcacttc atttttgtca gataaataaa
    16921 aaacaaaaaa agttccatct ttaaaacacg ctattctttt actttttctc acattctcta
    16981 agaaaagaaa aggagaatga atttttgaca attcgagcaa aagtgattat caaatatgta
    17041 aaatcagcta cttttttctt aaacgtttct ttatactaaa tataaataag tgtttcatga
    17101 aaggagaact agatgaataa ttggcttgag tattttcctg aaaacgtttt ggaacgcggt
    17161 tacagctatc atctgcacgg ttttgtccgt catttgaact atacaagcaa gtacttgtcc
    17221 gcaacagttt ctggcacgga agattataag gtagtgatca cttgggatga aaagaccaac
    17281 atgacttgtg attgtctata tgccatagaa ggaaaaaaat gcaaacacat ggctgctgtc
    17341 ttattcgctt acgaagaacg tccaataaaa aaatccaact actcactttc cgaactctcg
    17401 tcccttgttt cctccgctag ttcctctctt gtacgggagc ttttaacaga gattcttata
    17461 gaacatccgc aatttatcga acgcttcaaa gttaagatgc catttcatgc aataaattat
    17521 tcagataaat taacaactat catccataaa tacgatcata ttatcaagaa aaacaaaaac
    17581 aggaaaacag cgaagttcat catggagatg cgaaagttca tccaagaagc agttgaatcg
    17641 ctcattcagc aaaatgccta tcttcccgca ttcgaattga tcaatgaagt gattgctact
    17701 ctcgaaacat tctattggga gcctgaggat gaacgaaccc ttcttctgat agaggactgt
    17761 tattatttat ggaaagaatt attagcagaa gcacctcatg cagaaaaaag gcaaatgttc
    17821 tcttggttcg tctgccaagt cgatcatact gacgcctctt attcaaaacg ctactccata
    17881 aaaatcctga aagaagactt tcgggaaaaa gaattttcta accaaaagaa aaaaatagat
    17941 aaaaaaacta aagaaacggt aaagaaagac gatttcaatg aaaaatagca agtaaacgat
    18001 aacaaccatc ctaaatccac aagaaataca gcagaccttg ctttaaaaac acgaatcatg
    18061 caaaggatga agtaaagaag acttaacgcg tacccaatat tagtcatttg actattaggt
    18121 acgcgttttg ttttataagt catgacaaat ttccttcgtt gctgggtaga ttttccggca
    18181 ttgttgatag attttatgat acttttctac attttgtaag agaggttcca ctgcatctgt
    18241 atattggata aaattctgac aacaactttc aggtgtgtcg aaccactcta atctttaaag
    18301 ataaatatta aaataacgat taataatatt aacagtcttt atttttcacc accaaaaacc
    18361 tatctttgca tatactaaat agacgtatat acacagatag gtttgtcttg tgaaaaataa
    18421 tgattatcct taccaaaaaa tgtttgtttt tctacatgtt cgtacttatc aaaaaatatg
    18481 atccttattg aaattactgg ctatttatat tccaacgact tcttgtgtta atttttgtta
    18541 tctcatagta aattatgttt cattttcact taatttagtt gtcacgtgtt cgtcaattcg
    18601 taaaaacggt aaatatataa acacacttgc ggctataatt aatatttgta aaacagctgc
    18661 tcgccagtct ccggctgttg ctaaaaatcc cgatataact ggtggcgtag tccaaggaat
    18721 ttgcactact acatgattaa ttaatcccac agcagttgca aaatatgcta ctgtcaaaga
    18781 aaaaatcggt gcaagcacaa agggaataat cagactgata ttaaatacaa taggcaatcc
    18841 aaaaataata ggttcgctta tattgaatat agcgggagtt accgataatt tagcaaagtc
    18901 cttataatct tttctacgac tgaagataaa tattgcaatc aataaagcaa tcgtatttcc
    18961 cgatccaccc attaccgcaa aggtatcttt aaaggccata ttgataatat gtggaatttg
    19021 ctctttgttt gcataagctg ccatattttc ttgcatattc tgaatcaaaa acggatctaa
    19081 caatgaacct gatattactg cttgatggat accaaatcca aaaaatagat tagcaataga
    19141 tgtaattaac aaaaatcctg gtaaaccagt tgtaatttta cttaatggct ctgtaactat
    19201 cgtggtaatt agcttattta tatccatatt aaacaattga atgattataa atgacaataa
    19261 agcaaacgag gtaatcgtaa taatgattgg gattaataca ttaaaagact ttaaaacagc
    19321 gggaggaata tttccggata gctgaatctg tagtttctta ttattagaaa gtttaataaa
    19381 taattctgta gccgctaatc caacaaatat acctacaaac attcctgaag ctcctgtata
    19441 ggatataggt atcacacccg tcacttcaac agctttatcc gcattttcag gagtaaacat
    19501 catctgcata ggtgttaata caacaaatgt cgaaatagca actaataccg ctgcaatatg
    19561 atttgtataa tttctgcttg cacacatatg ataagatact gctgctacaa ctaataatgt
    19621 agttatgctt aatgtaccat tggcaattga agtaccaatt tgttgtaacg tggtcaatgt
    19681 actagtatcg acaatttccc ccatgaatcc tgttggttct aagatcactg aattaataag
    19741 tgtcacaaat cctgctaaca tcataaatgg cataaatgta gcaaacgcat ctcgcaaagt
    19801 tcttaaatgg atctgattcc ccaacttagt cgcaactata ccaaattttt caaatacaat
    19861 gtccttcctg ctacttttgc ccatattttt tctcctttat gcttattgta atcgattact
    19921 ttttccaccc aaataataca tcacttatca aaaaaagtaa atacttttaa ttaaatatat
    19981 atatcttttt tagtataaaa aaataagctc tgaaaaaata attttcccga gcttattttt
    20041 caacaaaaac agcttcaaaa acaaagtctt gatataaatg tctcgatacc gaatactcga
    20101 aaggtttacc agacgataga gcaacaactt tgtctatctc aatcataaat tcacctggat
    20161 ctatttttaa tatttttgcg tcaaattcat tcgcagtatc tcctttgata cgtatgtgcg
    20221 aagtctttat ttccagaccc agttcatttg tgatataagc atatacagat ttctttaaat
    20281 gttttggttc caagcctggt atgactgcta atggcatgaa tgtttgctca atcgagtatg
    20341 gttgacaatc aataaaacgg cttcgtacaa tattatatac atagtctttt acactgatac
    20401 ttaatttatc agctactttt ttcgatggaa gttcaattga gaaagtatgg atttctgaat
    20461 ctacgtgttc ttcaccaacc acgctaaaca aaccttcgtt tggaccaaga actcgtgagt
    20521 tatcaattgg tgttctgacg acttcagtcc catacccact tctacttcgc aaaactcctt
    20581 cggaaacaag taagtctacg cctttcttta ctgttaactt gcttgtatga taaataactg
    20641 ccaattcatc tcctgtagga agtttctcac caattttata ctctccattt acaatttttt
    20701 tctttatatc taagtaaatt tccatatact tcgcaatttt tttcaatggt gaccctccaa
    20761 tcaaaaacat atcttcatct atactttaac ataaataaac cgataaaatc cacatcaaaa
    20821 ttatacacac aataaaaaag atatatatat ttaaacaaaa ctatttactt taagttgtaa
    20881 gcggtgttat cattaagtgg aaacaggagg taaaaaaatt atgacaacta tgaaaaagct
    20941 aacttttcca gatgattttt ggtggggttc cgcttggtca gctgagcaag cagaaggaaa
    21001 aggagaaaca ggaaaagcag agactatctg ggatcgctgg tttaaagaac aaccttaccg
    21061 gttttatcaa cgaatcgatt cctctattac aaccgatcac atccatcaat ataaagaaga
    21121 tatagaacta atgaaaaaaa ctgggcataa ctcctttcgc gtttctattt cttgggcacg
    21181 aatgtttcca aatggcggta ccggaaaagt aaatcccaaa gccatttctt tctatcgaaa
    21241 attattttcc gaaatgaata ataacggaat caaagtattt gccaacttat atcattttga
    21301 catgcctgct actcttcaag ataaaggagg atgggaatca cgtgaagtag ttgatgctta
    21361 tgttcattac gctgaaactt gttttaaaga atttggtgac ttagtttgtc attggtttac
    21421 cttcaatgaa ccacttgggc ctattttagg tacttatctt gaagattttc attatccaaa
    21481 cctcattgac tttaaacgcg gagcgcaagc agcattcttc actatacttg cacacgcaaa
    21541 agcaatacaa gtatttaaag aactagctct tcctagtaaa ataggcgtta ttctaaattt
    21601 gagtcctact tttcctcgta gtcaaaatcc tgctgatgtg aaagcagcag aaatcgctga
    21661 tttattttat acgcggagct ttttagatcc tatggttaaa gggaattttt ctaaaaagct
    21721 tatcctgtta ttacaagaat ataatcagct tcctgaagat tatacgaaag aagatcttgt
    21781 atgtatctac gaaaataccg ctcaaattct tggactaaac tattacgagc cacgtcgcgt
    21841 caaatctcgt tcttctatgg tggatacatc tagtccattt ttaccagagt ggtttttcga
    21901 accacatatc atgcctggac gacgaatgaa cgaataccgg ggatgggaaa tttacgaaaa
    21961 aggaatttat gatttgtgca tggatatcaa gaaccattat ggaaacatcg aagcatttat
    22021 ctcggagaac ggaatgggcg ttgcggatga agaacgtttc atggatgata cagggcaaat
    22081 aattgatgat tatcggattg agtatatcaa agatcatctt gcttacttat ggaaggcaat
    22141 aaatgaagga tgtaacatta aggggtatca tctttggaca tttattgatt gctggtcatg
    22201 gatcaacgcc tataaaaacc gatatgggct ggtttcactc gaccttacta ctcaaaaaag
    22261 aacaattaaa aaaagtggcg aattttacaa gcaattaagt gatacaaatg gatttagata
    22321 cgatgtagac ttgttagttg aataatattc taaaattcca cttgaagtga acacattaga
    22381 aaacaagaat aatgagaaat accaataaca aatacttttt cttcatactt catttatact
    22441 tgtcagtata agagactgtg acaaatgtag tgatcctcag aagttaggtc tggaaatcta
    22501 acttgagaag atttttttaa actacgtcca gtctttttta tttgtttaaa attgaagttg
    22561 acaagtcttc attaaaaatt tagaatattc acaagtttga cgacaattat gaaaatttat
    22621 gtcgtgctaa aggagttttg ttattggatg aaaagttaga tataaaaact gcgatcaaag
    22681 acacgttacc taccgttttt ggttatatcg gtattggact tgcatttggt atcgttggga
    22741 aagctgccgg gtttcatccg ctggtcgtca cgttgatgtc cctgctgatc tatgctggtt
    22801 ctgcccaatt tatcacggtc agcatgcttg ctagccatag tccactgctc tccatcgttc
    22861 tctcgacctt tctagtcaat tcccgaatga ttctgatgag tatgacgatt gctccttatt
    22921 tcaagaaaaa tcggctgctt caaaatctgc tgattggcac attgctgaca gatgaaagtt
    22981 tcgcactggg aatgaataaa ttgaactata ctggacaaaa gttaaacttt cgttggatga
    23041 atacagctaa ttggatttct tatttgacgt gggtgggttc ttctcttgtt ggcgcacttt
    23101 tagggaattt tattactgat ccgaaaaagt ttggtttgga tttcgccatc gtcgctatgt
    23161 ttatcgggtt actttatctt caagtcattt ctgatagaaa cacctctaaa cgattacagt
    23221 tgatcctgat tggtctcact ttgattctgg tatatgtcgg actgattttt atcccaagta
    23281 acttagtgat tttagttgta acactgattg ggtgcggatt gggggtgtgg atcaaacatg
    23341 ccttcttttg agtttacatt gctgactatt ttaggttgta ctttagcaac ttggctttct
    23401 aggatcctac catttgtctt actgaaaaaa ttcgatttgc cgcaaccgtt gcttgagtat
    23461 ttaagttttg tcccgatcgt cattatgtct gctttatggt tcagcagtct atttacccaa
    23521 aatatcggac atctgccgca gatcaatctc gaaaatgctt tggcttcagt tcctacactt
    23581 ttagctgcta ttctttcaaa aagcttattg gtgatcgtct tggtaggaat tctttccctt
    23641 tcgttgattc gcttattata aataaaaaaa caagagctta tggcaaatta ctgtcatgcg
    23701 ctcttgtttt tatcaaagtc aaataagaaa aaaatcggcg tattcttgct tatccgttga
    23761 acggaccact aacaacgact tttcctaata tatgtcctgt ttctacgtct ttggttgctt
    23821 gtttcaagtt cttcgcattc atcccatctg aatagacctt gctcaatgta ctgcggataa
    23881 tcccttcatt tgctaaagca gcaatgtgcg ctaaagcttc tccttgcgtt tcgatttcat
    23941 agttatagtc tgttttagcg aacatatact cccagtcgaa gctgatagat tggtttttcc
    24001 aaacacctat atctaacggc tctttgatcc ctacgatcgt tcccacatgt ccaaatggtc
    24061 ggacaaggtg tttgatttga tcaaagtagc gggtaatatc gaataataca gcgacatact
    24121 ctacttgatc gattcctaat tcacgaaaac ttttacttaa gtcgtgatga taatcgattg
    24181 gatagtctac acctgttttt ttcagccatt caaaattctt cggacttgca gtcgcgtaga
    24241 cttctaggcc agcccaatgc gctagttgat tcaaaatcga accgacgccc ccagctccgt
    24301 tgatcactaa gatttttttc ccgcggttgg cattttcttc tggagtcaga ccaaattttt
    24361 caaataacaa ttcataagcg gtcaatgagg tcaacggcaa ggctgcggct tcttcatctg
    24421 aaagattttt tggtgcaaga gcgacaatac gttcgtcaac cagttggtat tcttgattgc
    24481 ttcctgctct tgtcgttgta cctgcgtaaa aaacgcgatc gccgacagaa aatttttgca
    24541 cttcatcacc tacagcaaca actttcccga ctccatcaaa ccctaagata cgtaaagcat
    24601 ttcttatccc gttatcttgg cgaagtttcg tatcgacagg attaacagat actgcgttga
    24661 cttttaccag aagatcccgt gaactgggta caggtatctg ttctttttca tcaataaagc
    24721 tttttggatc atcgatcgac aacccttcat aaaaaccgac taccttcatt aattcctttt
    24781 ctgacatacg aattcctcct tagcttcatc aagttggagc atccatacag ttttcatttc
    24841 aatatatata gtatgctatt tgttccactt ttattcatca taattattct cttacttaaa
    24901 gtaaactatt tgcacttttt tggttaaaaa agaggttgcg acaagacttt tgtcacaacc
    24961 tcttttaaac ggtgttcaat caactgaccc tcggtattcg ctcaaaaaac gacaaaatat
    25021 gaggagctat ttttgtttgg ctgttcttat tacttgatgc aggacagctt tttcacgacc
    25081 tctaattcac ggtgttcaag caagaaatca ttgttcactg aaaagcgttg gatgttcttt
    25141 ttcaaaggtg tctaagatct ctcgtacttc atcagtagat ttcgtattca tcaactgaac
    25201 tcgcaagtca tttgctcctg gaaatccttt gatatagatt ttaaagaaac gatgtagtcc
    25261 tgtgatggaa cgcgggatga tttctgaata atgatcttgc agatctagtt gcagacgaag
    25321 tagtccgatc agttcttttt ctgtatgttc cctcggttct ttttcaaatg cataaggatt
    25381 cttgaaaatc ccccggccaa tcatgatgcc atctacgcca tatttttcgg ccaattcttc
    25441 tcctgtctgg cgatctggga tatctccgtt gatcgtgatc agtgtttgcg gagcgatttg
    25501 gtcacgtaaa gcaatgattt tagggatgac ttcccaatgt gcatctactt tactcatctc
    25561 tttacgcgta cgcaaatgga cagaaagatt ggctatatct tgatgaagta ggtgactgat
    25621 ccattcttcc atctcagaca tttccgtata gccgatacgc gtcttgacgc tgacaggcaa
    25681 accgccagct ttagccgctt caatcaattg tgccgctact tctgggcgaa ggatcaagcc
    25741 actgcctttt ccacgttctg ctacattagg gactgggcag cccatgttga tatcgattcc
    25801 ttggaagccc atttctgcca tagcaatgct catttctcta aaaaattctg gcttgtctcc
    25861 ccaaatatgg gcaaccatcg gctgttcatc ttctgtaaag gtcaatcttc ctcgtacact
    25921 gtcttttcca tctggatgac agaaactgtc tgagtttgta aactcagtaa agaagacatc
    25981 cggtgctgct gcatgcttta cgacatggcg aaatacaaca tctgttacat cttccattgg
    26041 tgctaaaaca aaaaaaggct ttggcagctc tttccaaaaa ttagtagtca tttatttgtt
    26101 actctccatt ctatcaagca gtgaagtcgc aatctttctc aaaaacattg tcattatact
    26161 aggaaacaaa aaaagaagca aagtgaacag cttaaaagat gtttctgatt gagcaaaaaa
    26221 agactgtgac aaaaatctta tcacaattcc ttttccggaa taaatggtgt tcaaaagctt
    26281 gcatctgcgg taataag
//
LOCUS       DACECR010000032        26182 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.032, whole genome shotgun sequence.
ACCESSION   DACECR010000032 DACECR010000000
VERSION     DACECR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.032"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            168..1208
                     /locus_tag="IWO17_001247"
     CDS             168..1208
                     /locus_tag="IWO17_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0290133.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(1260..3404)
                     /locus_tag="IWO17_001248"
     CDS             complement(1260..3404)
                     /locus_tag="IWO17_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0290134.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(3761..5134)
                     /locus_tag="IWO17_001249"
     CDS             complement(3761..5134)
                     /locus_tag="IWO17_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0290135.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(5134..5529)
                     /gene="rbsD"
                     /locus_tag="IWO17_001250"
     CDS             complement(5134..5529)
                     /gene="rbsD"
                     /locus_tag="IWO17_001250"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ0290136.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(5557..6474)
                     /gene="rbsK"
                     /locus_tag="IWO17_001251"
     CDS             complement(5557..6474)
                     /gene="rbsK"
                     /locus_tag="IWO17_001251"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ0290137.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(6582..7595)
                     /locus_tag="IWO17_001252"
     CDS             complement(6582..7595)
                     /locus_tag="IWO17_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0290138.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(7676..8275)
                     /locus_tag="IWO17_001253"
     CDS             complement(7676..8275)
                     /locus_tag="IWO17_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0290139.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            8461..9162
                     /locus_tag="IWO17_001254"
     CDS             8461..9162
                     /locus_tag="IWO17_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0290140.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            9172..11883
                     /locus_tag="IWO17_001255"
     CDS             9172..11883
                     /locus_tag="IWO17_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0290141.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            12334..13962
                     /gene="treC"
                     /locus_tag="IWO17_001256"
     CDS             12334..13962
                     /gene="treC"
                     /locus_tag="IWO17_001256"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ0290142.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14022..15203)
                     /locus_tag="IWO17_001257"
     CDS             complement(14022..15203)
                     /locus_tag="IWO17_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0290143.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            15455..16978
                     /locus_tag="IWO17_001258"
     CDS             15455..16978
                     /locus_tag="IWO17_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0290144.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(17047..17775)
                     /locus_tag="IWO17_001259"
     CDS             complement(17047..17775)
                     /locus_tag="IWO17_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0290145.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(17788..19068)
                     /locus_tag="IWO17_001260"
     CDS             complement(17788..19068)
                     /locus_tag="IWO17_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0290146.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(19209..19811)
                     /locus_tag="IWO17_001261"
     CDS             complement(19209..19811)
                     /locus_tag="IWO17_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ0290147.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            20056..20262
                     /locus_tag="IWO17_001262"
     CDS             20056..20262
                     /locus_tag="IWO17_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290148.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            20574..21293
                     /locus_tag="IWO17_001263"
     CDS             20574..21293
                     /locus_tag="IWO17_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0290149.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(21374..22057)
                     /locus_tag="IWO17_001264"
     CDS             complement(21374..22057)
                     /locus_tag="IWO17_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ0290150.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            22275..24461
                     /locus_tag="IWO17_001265"
     CDS             22275..24461
                     /locus_tag="IWO17_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0290151.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            24807..25217
                     /locus_tag="IWO17_001266"
     CDS             24807..25217
                     /locus_tag="IWO17_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0290152.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            25231..25587
                     /locus_tag="IWO17_001267"
     CDS             25231..25587
                     /locus_tag="IWO17_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ0290153.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         8777 a         4642 c         5139 g         7624 t
ORIGIN      
        1 ttcggaaaaa gaggttgtga caatattttt gtcacaacct ttttttcacg gtcttttatt
       61 agaatatagt aaattttttc tcgttactca cggaataagc tgttaagaaa acggtttcca
      121 tgtatgctgg aatcagtgca aatgaaagaa aagagggtgc aaggaacatg gaaatcaatg
      181 aacaaaattt tggcgaaaac tatcacttga ttacgatcac gaatcaaaat gggacatctt
      241 tgtctgtcag tgatcttggg gcacgggttg ttcattttca aacaataatc gataatgaaa
      301 aacgagagtt gatcttaggt tttgatgaag caaaagaata tctagaaaaa gacccttata
      361 tcggtgcttc aatcggacgt atggctggac ggattacgaa tggcacattc aaattagatg
      421 atgaaacgtt cgaagtaaaa acagacccgg acactggtca ttgtttacat ggtggtgcac
      481 ctagctttga agccaagcag tggcattata cagtcatcaa tggtgaaaac gaagcatcgg
      541 tcattttcta tcagactagc ccagatatgg aaaacggatt tcctggaact ttagatgtag
      601 aagtacggta tacgctgacg aacgatgata catggcgagt gacggtccaa gggatcagtg
      661 accgcaaaac agtattcaat cctacaaatc atgtctattt caatttaacc ggtgatccag
      721 cacagtcaat cgacgaacat atactttggt tgaatagccg attatttgct acgctcaata
      781 aagatacaac gcctacagga gaagtagttt cagtaagtgg cacagcattt gacttccaac
      841 gtcctaaaaa attaggtgag gtctttgcca gtgattttcg ccaatcaatg atagtgggcg
      901 gcatcgatca tccatttttg ttgaaaaaac aatctggttt actaaatgct gcggaattga
      961 caagcccaga tgaaaaagta tcgatccaag taaaaacaga tgcttcagga atcgtgattt
     1021 ttacagcaaa ttttggagaa gatggaccgg aaatgcgcgg gaataaatta gtaaatcatg
     1081 gcggtatcac atttgagact caagaactgc caggagcgga gcgacttgaa agttttggag
     1141 atgtcactct ggaacctaac caagtacgcg agtttgtaac ggaatacaaa ataaatataa
     1201 agaaatagaa taaattaaaa agggagtgtg acagtgtcac atcccctttt taatttattc
     1261 tatttcttta aaggcagatc aagccagaat gatttgtcta aacttaacaa ggttagtaca
     1321 ggaacagttg cagctttaac tgtacgagta gttgtttctg gtatctgtct tgccgtaaaa
     1381 ttgttccaca aatcccagat tgaagcgaca cggaagatgg aagaagcgtt gttgacgcgt
     1441 tttatcgttg aaaattctaa tcctgtgatt ggcacgccat ctgttgtcca tttgattggc
     1501 acatcgaatc gcttagacat attgaggatt tcagcgtttg cttcttcagt tgttgctgct
     1561 tcttttttcg gttcatcata ttttgtcagg tcgtacgctt cgatgatcgc gttcaatttt
     1621 tgtgaatatt ggcgatctgt agcataacgt ccttccaaat atttggttgc ttctttgtag
     1681 tcttttgttt ccgacttcca agttggtttg tagaaatcag gattgccagt cagtccattt
     1741 ttgatcaagt cggcatagtc ttccaaactt tccttgtaag aaggatattt acggaattgt
     1801 gcgcggattt ggtagctttg tccggaacta tcttgttcaa gtgtattgaa ggtcacgctg
     1861 cttcctttat atgatccttt gataccaaaa aggttatgat ttggttcgct tgataataag
     1921 ctgtttccag atccactttc aaggatagct tgagccatca tgactgatgc gtagattcct
     1981 tctttttgcc cgatttcctg tgcatcatct gcaatctttt tcaagaactg atcagttgtt
     2041 tggttttgac tgaaatggaa cgagccatct aatccatcta aaatagatga gccgcctgtc
     2101 agcgaacctg aagcagcagg taaacctgct aagtttactc ggacagcaaa atcaggcgtg
     2161 aaatctgaaa tagaagttac tcgaacttta tctcctggtt gaggcgcatg gataaattgt
     2221 ccgttcccaa tatagatacc tacatggtga gtaacaccgg aagaaggtcc ccagaagacg
     2281 aggtcgccag gttgggcctg acttactgga atctgcgtac ctgcatattg ctgttcgcct
     2341 gtccagccac cgatataatg accggttgct ttcatatata cgtaataagt caaaccagaa
     2401 cagtcaaaag tatttggacc ttttgctccc catacatatg gttttccaag atgcttgaat
     2461 gcttctcgaa cgattgcttg ttggacattc gttgtatttg taggcaatac agcatcaatt
     2521 ggtgatttta caacaaaatc agaagaagca gaagatggat tctctatacc gctttgattt
     2581 gctgtatttg gacgagatga agcattgttc gttccgccat tcgatgaacc attgtttgga
     2641 tggtttggtt ctctctgagt atttccattg ttattcgatc cagatgggtt tgtttgattc
     2701 gaagaattcc cgctgttcga attattgtta ttattgtttg aattatttga accattatta
     2761 ttgttgttat tacccacatc tggtttgttt ggcgtttccg gatctggtgt actagggact
     2821 tcttcactag gatcagttgg tttctctgtt gaagtatccg gtccagcttt aaattctgca
     2881 gtaatagata aattgttcgt acctactgta aaggtctttg catcataatt tacttctttg
     2941 gcaccattaa cagcgactga agcaatttgt tcaccttcag ctggcgtaat agtgtaagtt
     3001 accttcgttt ccgctaataa accttctacc gttccacttt ccgtaatatc caaaggttgc
     3061 tgttcagggg aatccgctgt cgaagcagtc agttggatct taccaatcaa agaaggagca
     3121 attgtaaggg aatatgtctc ggctggcttt tcattttctg atggatcagc cggatcaatt
     3181 tcagttggct cggtcgatcc tgttgttgag ttagttgttc cagttgggtc ttctgtaaaa
     3241 gaatccgttg tttcactagt agctgagctg gatgtagtcg tatctgttgt gactgtttga
     3301 tcagctattt gattatctac ggcaaatacg gtattggtcg acaaaacaga cggagcaatc
     3361 agccaagtag cagctagcaa acgtatcgtt tttttatttt tcataataaa ctagtccttt
     3421 tcctttcatt gattttctgt cttattcagc attcattaga cattttttgg aacagataat
     3481 cgtgtcgaaa agctttttct tttcataaat ctgtctgtgt attctaaata aaacaatttg
     3541 taaataagca acgagcattc attcgttttt ctttgacgtt cattttattg catagaccac
     3601 ctttgtttgg ggataaacaa gtggggataa aaatggttat gcatatgtaa attataccaa
     3661 agtttggaaa gtatcacaaa aatttataaa aataatgatc ctaaaaagaa aaagactgaa
     3721 atatgtgata aacatctcat acttcagtct ttttcattaa ctattcataa gacattaggt
     3781 cttcatcaaa cccgctaacg agagtagtga tcattgctac gactagacta acagccagtc
     3841 ctgctaagta ccagaaaaaa gtaccggaaa tgaatgcagg caatgtggtc agactaccaa
     3901 atacaaaagc gatcgctttc acttgggcaa atcccattac agctccacca gcagctgcac
     3961 caatgcaagc agcaacaaat ggttttttca atgggatcgc aattccgtaa acagttggtt
     4021 cggtcactcc cgcaagtaaa atcgaagcaa aagcagaacc ggagatcgaa cgggtttttg
     4081 aacgtttact tcgcagaaat acacctaaag ctgcaccagc cattccaaaa ttggctgctc
     4141 ctgtcaacgg aacgattcgg tcgaacccat aagcagagat attactgatc atgacagggt
     4201 ttacagccca ttggatacca aatattacca aaatattcca accaccagcg atgattgcgc
     4261 cactgatcgg tccgttaata tccatcatcc aagtaattgc ggcagctaaa ccttcgccaa
     4321 ggtaaacacc cgcaggcccg aataccatta acgtcaaagg aaccgtgata actaatgaaa
     4381 gtaaaggtac aaatactaat tgcatatttt cgcgaatata ttttttgagg aatttttcaa
     4441 caaaagaata aaagaaaata cctaaaatcg ccggcatgac agtagacgta tactgcatca
     4501 gtactacagg aatccccaga aaatcagtag ctgctttgcc actgtctgaa agtaaagagg
     4561 tcagattcgg ttcaatcagt gctgcagcaa tgacagccgc aatatatcca ttcactttaa
     4621 acgttttggc agcagaaaaa gcaagaaaga ttggcagaaa ataaaagacg gcattcgacg
     4681 cagcagacaa aatatgataa gttccgcttg tttcactaag ccagccaatc gtagtcgcta
     4741 aaacaaccag accttttaat ataccagaac cggcaaacag cggaatgatc ggcgtaaaga
     4801 tttccgatat caaaccaaat agtttttcga tcggatttag cttttctttc ggagctgaat
     4861 gatcgatttg acgcgtcaat tccttgtaag caggagccaa tccctgatcc atgattattt
     4921 ctgcatatct atcgagtaaa atgaaatact taatggaagg gatttgctgt tttaattttt
     4981 ctgcattggc tactggctgt tttttcaatt tgatacgaag ccgagtcgca catggaagga
     5041 cagaatcaat attgtctttt cctcccaact gttcaatcag catatgaatc tgctcggaca
     5101 gtaccatctg ttcaggattc attgtcgttt ccattagaag accactccac tcactaatat
     5161 aatattggcg taaggtgtca tttcgcctgt acgaataaat gcatgacagt tattcaactc
     5221 ttttttcatt tcttcatgtg ggataaagct aatcggtgta tctggtaaca ataatttgat
     5281 tttttccaat atttccgggt tcattgtctt tatttcttcc gccaattcaa tcgattgtac
     5341 ctcaagttct tctaaaacgt tttctaaaac agataaaaaa cttggaagac cgtttgagac
     5401 agccaaatct attttttcag taccaaaggg gacaggcatg cctgcatccc cgatacttaa
     5461 tttatcaaag tgccccattt gtgcgattac tcgtgaaata tctgaattga ttacttttcc
     5521 ttttttcatg tttgtttctt cctctcattg ttaattttag actgctgatt gttctaaagc
     5581 ttcgcggtaa gggatagaag gttgcgcacc ataagactgc acagcaacag aagaggctag
     5641 atttccataa aggatcgctt ctttcaagtt actaaggtct ggctgaagtg tgctgcttaa
     5701 tgcgccaata aatgtatcac ctgcggctgt tgtgtcaacg gcatccactt taaaagcagg
     5761 gacgataccg tgttcttttt ctgtatgata gaaagcgccg gcactgccta gtgtaatgat
     5821 tactgttcca atacctaatt catggagttt ttcggctgct gcgaccaaac tgttatgatc
     5881 tgtcactctg attcctgtaa tgatttctgt ttctgtttca tttggtataa tgatatctgt
     5941 cttttcaagt agttcagtag gaatcgtttc acgagctggt gcaggattca agatcgttgt
     6001 ttttcctgcg tcttttgcaa tagaaaaagc taggatcgtc gcatctaagc tactttcaaa
     6061 ctgagcaata ataaagtcac tgtttgtaat cagttgcttg ttttttagga cttcttgttc
     6121 atggaaagca ttatttgccc ctgcatggat caagatacta ttttctcctg ctgcgtctac
     6181 cataatcatt gcttgtccgg ttgtggcgcc ttggatttca gctatcccag atgtatcgat
     6241 gttttctttt gtcagtagat ctaataactg ttgtccttcg ctgtcagccc caacgcctcc
     6301 aataaaagat gtccgtgcgc ctgaacgttt ggctgccact gcttggttgg cacctttacc
     6361 tccgccagcg tgaaaaatct catgcgcatg catcgtttcg cctggcttgg gcatgttggt
     6421 taaacgaagg gtagtgtcca tattaatact tccaattact gtaaccgtgt tcattttaca
     6481 aacctccaac tatctcattt attttgatgg gaaaattatt ttttaaaacg ttttagtaaa
     6541 acgttttaat agttcgataa aaatatagca caccttgaaa gctatttcaa gggtgcagtt
     6601 gaaaatcttt tttctaatcg tacaggtaat cttttttctt cccattcttt ttctgggaat
     6661 tggatccgat ctaacaataa cttggcagat gtttgtccca attcgaagat tggttgtgcg
     6721 atcgttgtca gtttcggctt gatatactcg cacatatcta tattatcata gccaataatg
     6781 ctgtagtctt cagggatgct ttttccagct tcctctaatc cgcgatataa tccaaaagct
     6841 aattcatcat ttaaagcaaa cacaccagtt gcagcagaat caagaagttc tgctgtgatc
     6901 tggtagccac cttgttttga gaactgtgta ggtaagagga tcagttggtc gtgagggatt
     6961 tggtaaacat caagagcaga tttaaacccc tcaatccgag cgtggacatt ttccggtgga
     7021 ttttcaggat agacaagggc gatcgtttga tgtcctagac taagcaagtg catgcccgct
     7081 aggtaaccac cgcgaaaatc gtctgttcgt acggcatcac taaatccttc agattttttc
     7141 tgatccaaaa caatgaaggg ccggccttgt tttttcaaat tttcgttgat tgcatctgta
     7201 gagacagcag aagtcgcgat aatgaatcca tccacacctc gacgtgtgag ctcggctaga
     7261 tattcgattt ctttttgatg atcagaatca gcattacata atatcgtgac gaaattctgc
     7321 ttatacaaga tatcctcaat tcctcgcatc agtgtactga agaatggatt cgtgatatca
     7381 ggaacgagta caccaattgt tttcgtttcc ttggtaatca tttgtctagc aaaatagtca
     7441 ggctgatagc ctaattcttc tttggcagct aaaactttct gaacagtttt cggactgaat
     7501 ttctccgtat ttccatttaa tatcaaggag acagttgtga ttgaaacgcc ggaaagttta
     7561 gcgacttctt tgattgtgat cttttttttc accatgttca tactcctatc taaatcctgt
     7621 ggttattttc ccatgaaatc aaaaagaagt aaatgaaaat cacgtgtgcc agtacttatt
     7681 tgctttgttg gatagcttcg attatttgtt tcgttgagcg tactcgtgca gttttcggaa
     7741 atatcttttt tatgaacaat tgatgggatt ctttgtcacg atcactgcaa gcatcttcag
     7801 caagcacgat atggtacccg tgttgaaagg catctaatgc agtagcatat acaccgtttg
     7861 ttgtcgctac accgcttaaa atgatagttt cgattccacg ccggcgaagt tgaagatcaa
     7921 gagaagtacc aaaaaaagca cttggattgt attttgtgat agtcaacata ttatctgtat
     7981 ctttcaaaga atccttcaaa agtaaatcag tgaattctgg aggtacagaa gcagtcattc
     8041 cacccatatc agtttgttga ctcaaataag ggaaagtgga gatgtctacg ttcactaaaa
     8101 ctttcaaggc agcggtacgt ttcaatgttc ttactaacaa atcattgttt gtcagaatag
     8161 atgctgcagt gtgtggagag agtgtaccgt tatttacgat ctctttttgc atgtcgatcg
     8221 cgacaaaagc agtttttgat aaatcaaaaa tcatattttc ctttaaatca ttcatgatgt
     8281 tccttctttc aaataacaat gttttttatg taagtaaatc ataaatcgtt tatttgtcaa
     8341 ctaaattaaa ggattccttt ctttagtcta gagaaattca gtggtaaaat aaataagtgt
     8401 gagaaaacgc attcgatgca tagatatagc gagaaatatc gtgaaaatgg aggaatcaga
     8461 gtgaagtatg tcgaagtaaa aaatgaatac aaacgataca aaatgggaga aacgaccatt
     8521 acagcaaatg acgatatttc tttttcaatt gaaaaaggcg aattagtgat tatacttggt
     8581 ccaagtggag caggaaagtc aacgattctt aatattttag gtgggatgga tacacctgat
     8641 gaaggtcaga tcattattga tgacacagat attgcgcaat tttcagataa acagcttact
     8701 gcttaccgac gaacagatgt agggttcgtt ttccaattct acaatctcgt tcctaatcta
     8761 acagcaaaag aaaatgtgga attggcaacg gaagtttctc ctgatgcgct tgatcctgta
     8821 gaggtattga ggcaagtggg cctagctcat cgattgaata actttccgtc ccaattatca
     8881 ggaggcgaac agcagagggt ttcaattgct cgtgcactag ctaaaaatcc caaacttctt
     8941 ttatgtgatg aaccgacagg cgcgctggat tttgaaacgg gcaagcaagt attgaaatta
     9001 ttgcaaaacg ctagcagaca acacggcaat accgttttga tcattacaca taattctgct
     9061 ttagcgccca tcgctgatag agtcattcac attaatgatg ctaaagtaag gtctgttgaa
     9121 ttaaatgatc atccttcatc cattgatgag attgtatggt agggggattt tatgaaaaca
     9181 agaacatatc ttaaagcagg ttttcgcgaa attcggcaat ctaaaggacg attcattgca
     9241 atcatcctta ttgtcatgct aggaacactt ttatttgtag gtgtcaaaac tgctggacct
     9301 gtcatgcaaa aaacgatgga tcactatgtt ggtacagcag gactttctga tttgcaaatc
     9361 gtttctactg gtggcttgac acaaaaagat attgctcaag cagaaaagat cccagatgca
     9421 aaagtagaaa caggaaaaca gatctattat tccaatactg gcaaaaacga agtgattcaa
     9481 atattttctt ataacaaaaa taacaagcag aacaagctac aattaactga tggaaaactc
     9541 ccggaaaaac caaaccagct tgtattggat gaaaaagcgg aagatgaagg gtataaaatc
     9601 ggtgatattt atcagattga ttcagatgaa ttgaaagaaa aagagtatac catcacgggc
     9661 tttgtccgtt ctccattgtt catcaacaat ttggagagag gatacgcgaa cgtcggaaat
     9721 ggtagcgtcg attattttgt ctatttacct gaatccgttt ttaaaacaga tatttattct
     9781 gtcttatatc ttgattttac aaacgtaaag aatttgaata cgtacagcaa agcttacaaa
     9841 gataaaatgg agcaaaatca agaaaaggca gaaaagtatt tacaagatcg acccgaagaa
     9901 cgcttagaag agctgaaaaa atcggccaac gaagaattag aaccggcaca gcaaaaaata
     9961 gcggatggca aagcacaaac aacgcaagca agaacacggt tagaagatgc taaaaaacag
    10021 ttagcgcaac agtcagcaac tatccagcag cttcctgaag cgcaacgaac aatcgctgtt
    10081 caaacattat ctcaacaaga agctcaactt gctgaacaag aaaggcaatt agttgagaaa
    10141 gaacgtgaac tggcctctgc agaggaagaa ttgaatcgaa atgcagaaaa aattgaaaat
    10201 ttaactaaac catcatacct atacaatgaa cgttcagaaa atgtcggttt tcaagaattt
    10261 ggtgatctgt ctgatcgtat cgctgcgatt gccaatgttt ttcctgtttt ctttttcttt
    10321 attgccgcat tgatcacctt tacgacaatg acacgtatgg tagaagaaaa ccgcagggaa
    10381 atcgggacat taaaggcgtt aggttatacg aaagttgaaa tcgcaaaaaa atatgcaatc
    10441 tatgcaagtc tagcctcagg gataggtatt gttttaggaa cgatattagg aacgaatctt
    10501 ttgccaagga tcatttttga gctgtctaac gagcggtatg atattgggag tgcgcttatt
    10561 ttttatgact ggccgccgat tttacaagca gctgccgcat tcttcattgc cgcatttggc
    10621 gctgcaatga tcgttttatt caaagattta agagaacggc ctgctgcatt gcttcaaccg
    10681 aaagcaccga agccagggaa acgcattttt cttgaataca tcactccgct gtggtcacgc
    10741 cttagcttta atcaaaaaat cagttaccga aatctcttcc gatataagtc aagaatgctt
    10801 atggcgatta tcggaattgc tggatgtgcc gggctgatgg ttgcaggcgt gggattgaaa
    10861 gattctctaa gttctgtttc agccaaacaa tttggaacga tcattgacta tcaagcaatc
    10921 gttacattgg attcatccac ggaagatagt cgtcaaaaag cagcggatat gctgcagaag
    10981 caagaaaaag taaatgagat cctagctatt catactcaaa ccgtagaatt gagaaaaaaa
    11041 ggacatgctt cgcaaagtct gacagtgatc gtaccaagta aaacagctga tttggatcgt
    11101 tttatccatt taactgatct tgaagagaac ccacgctcct tgcctaaaac aggaatcgct
    11161 attacgcaaa aagtagcaga actatttgac ttatctacag gagatcatat ttcgctttat
    11221 gatgaagatc aaaaagaatg gaaagtcgaa gtaaaagaaa tcgtgaaaaa ctatctaggt
    11281 agctttattt atatgtcccc tgaatattat gcttcagtta ctgatcaatc gatgatggat
    11341 aatgcatttt tgctgcgtac agataagatg acaacaaaag aagaagaaat gctttccgag
    11401 aaactgcttg ccacagacgc tgtgacaaat acgtctttca tgtccaaaca aatcgaaacg
    11461 caagaagaat caatggcgaa cttagactcg gttgtcttaa tctttgtcgt attatcagga
    11521 ctgctggctt ttatcgtctt gtataatcta actaatatca atatttccga gcgtgtacgc
    11581 gaattatcta caataaaagt ccttggattt tttgataaag aagtcacgat gtacattgtt
    11641 cgagaaaata tcatctttac tcttttagga atcatcggag gatttggtgt aggatatata
    11701 ttgactgact tcatcttgca acaagcgtct atggagaatg tcatctttcc attagtgatc
    11761 acatggtggg cttatgtctt atcagcagga ttaactatcg tatttacggt tattgtgatg
    11821 attgttaccc atttcaaatt aaagcatatc gatatgattg atgcgttgaa atcgaatgaa
    11881 taaaaacaga ggttgtgaaa tcagcggttt gacctgagta ttagagaaac aaacggcaaa
    11941 atagtttttc atattttgcc gttttttgag ctaataccga aggacagctg attgaacacc
    12001 atgaatcaga ggttgtaaaa aagccgtcct gcatcgagca aaaagaatac tcaaataaaa
    12061 ttagctcctc atgttttaca tttgagaaga ctgattgccg tagataaaag cttttgaaca
    12121 ccatttatag aagaaacaag aatattttgc tattttaaac aagaggatag acaaatacat
    12181 gtctgatctc ttgtttttta ttgggcaaca gacctcttag aatgtataaa gataaatttt
    12241 acaaacgctt tcggttcagt tataataata aatgataact tgtatatata agttgtggta
    12301 ggtaataaat cagttgaatg gaggtaaata tccatgacat ttaaagacaa agtcatttac
    12361 caaatctatc ccaaatctta caaagacagt aatggtgatg gaattggaga tttaaaagga
    12421 atcaaagaaa aactcccgta tctgaatgat ctaggcatcg acatgatttg gctaaaccct
    12481 atttatccaa gccctcaaaa agacaacggt tatgatatct ctgattacac agccatcgat
    12541 cctatctttg gtacgatgga ggaattcgaa cagctaatag ccgaagcaaa gaaataccgg
    12601 atagaaatca tgctcgatat ggtgctgaat cacgtttcga cagaacatga atggttcaaa
    12661 aaagcactag caggggacca gcattatcag gacttcttca ttttaagaga agagccaacg
    12721 gattgggtat caaaatttgg tggaagtgct tggtcgccat ttggagatac ggggaaatat
    12781 tatttgcact tatatgatcg gacacaagct gacttgaact ggcggaacca agcagtaaga
    12841 gaggaactat tcaaagttgt tcgtttttgg atggaaaaag gggtaaaagg attccgtttc
    12901 gatgttatca atgttatcgg taaagacgaa caattgaaaa ataataaaga aaatgatggc
    12961 aaacctgaat atacggacaa accaatcacc catacgtatc taaaagaatt gaaccaagca
    13021 acgtttggga cggattccac tatcatcaca gtcggtgaaa tgagctctac gacgatcgaa
    13081 aattgtatct tgtatacaga acctagtaga gaagaattat caatggtctt taatttccat
    13141 catttgaaag tcgattatga aaatgggaat aaatggtcta ctcctccatt tgattttgca
    13201 gaattaaagc gactatttca tacatgggga gaaaagatga gtgagggtaa cggctggaat
    13261 gctctatttt ttaataatca tgatcagcca cgtgcgctca atcgatttgt agctatcgat
    13321 cagtatcgaa aacaaggagc agagatgcta gcaaccatga tccatttgaa ccgtgggacg
    13381 ccctatattt atatgggaga agaaatcggc atgatcgacc cagactattc atcgatttcc
    13441 gactatattg acgtagaaag tttgaatgct tataagttac tccttgaaca aggattcaca
    13501 gaaaaagaag cgtttcgtcg gatcaaagca aaatctcgcg ataactctcg aacacccatg
    13561 caatggtcag acacagaaca agcaggattt actacaggga aaccttggtt aaaagttgga
    13621 ggcaagctag aagagattaa tgtagaaaaa gaactatctt ctgaagattc tattttgtcc
    13681 tattacaaga aattgattcg gctgagaaaa acatatccaa tcgttgcaca aggagattat
    13741 catgcttatg aagcaaatca tccggaagtt tatggctttc ttcgccaatt tgaaggacag
    13801 cagctgcttg tattaacaaa tttctatgat aaagatacaa ggatcgccat cccagaagga
    13861 ttcacagaag ccaatattct tatcgataat tattccgata ctgttcttca tcaaagtctg
    13921 gagctaaaac cataccaggc aattgctttg ctgaaaaatt aaaacaaaaa cacaaaacgt
    13981 cgttcctacg agctattctc gtaagatcga cgttttgtgt tttatttaaa ataatcttct
    14041 tcagaaaatg aagagttttc atactctcct ttatcgccat aaaatttatt gtagcgttgt
    14101 ttgaatttac ggaaaataaa agtaacgagt aatttgacta acgtatagat aggaatacca
    14161 aaaataacgc ccacaattcc cattagatca cccatgacaa gtaaaacgat taagatcgta
    14221 atcggatgga tctgtaaacg atcacccatt actcgaggaa tcaccaagtc accgtggaga
    14281 agttgtacgg caaaccagac aatgacaaat ttgacagcca taaaagtaga gtcttggaat
    14341 gcaatgatca aacctggaaa gaaggcgata aacggcccaa tatatggaat gatacataac
    14401 aacccagcag aaatagctaa aacactagca aattctaatc cgatcagtaa gaaacaaacc
    14461 cagtagactg ctccaaggat aaatgaagca atgatttgcc cctttagaaa tgaacccaat
    14521 tgttgattag caatctttgt caaattttta aaatctgtac gaaatgctgg tgggacaatc
    14581 gctaaaactg actgataaaa tttttgagga tctctcaaaa ggaaaaaagc gacaattgga
    14641 ccagtaaata aagtaataaa aatagctgta acagttgaaa aaatgttgct gatccgttgt
    14701 gcgccacttt cccaataatt tccaataaat ccagctatat cagacgtcac gtcgtcaata
    14761 gaagcgaaaa attgattaaa tgaatcggca aaaggtgttt tctccaaaaa ttgtcggaga
    14821 ctctcttgaa aatcttgaaa caaatctggc aaactactga tcaatgtctt tgcctgttct
    14881 tcaagaaaag gaataagcca tatgcttcca agccccaata aaaataaaat aatcacataa
    14941 acgataatga ctgacaagtt cctcgaaagg tgcttgctca aacgtttgat gactggttcc
    15001 aaaagataat acaagatcag tgcaaacaat aatgggggca agatagtagc gataatgatt
    15061 ccgacaggct tgaatataaa cgagatctga tggaaaaaat aaatgatcag cgcaagtaag
    15121 ataactattc ctaaaacata gtaagaggtt tttcctccta aaaagcggat caaacgattc
    15181 gttgtatctg tttctttttt cactcttttt cctcctttat ctataaattc attatagaca
    15241 aaagcgggta taaaaggaaa atgatagagg ttgattgcaa aaatatcgtc aatcattcat
    15301 ataaactttc tcatcaatta aaataagaaa acgcttgaca gttcttttaa ccatatgata
    15361 aattaaatcc tgttgctaaa acgtaactat tacggtttgc gaaaagcgaa tcggaacatt
    15421 caaatgaaag aagatgagaa gaggagtgtc ataaatgaaa cgattgatag gtatattggc
    15481 catgctggtt ttagcaggta tgttaactgc ttgcggggca agtgggaaag ccgaggacag
    15541 taaagaaaaa ttgagcgtga tgacgacctt ttatcccatg tatgatttta caaaagcaat
    15601 cgtcggggat gaaggagaag tagagttatt gattccagca gggactgatt cccacgatta
    15661 tgaaccttct gctaaagaca tggcaaagat ccaagataca gatatctttg tttataatga
    15721 tgaaaacatg gaaacatggg tccccgcaat ccaaaaaaca ttacaagaag gcaatgtcca
    15781 tacgataaaa gcgacagaag gtatgctttt acttccggga agtgaagaag gacatgatca
    15841 tgaccatgaa cacggggaag aaggccatac acatgagcta gacccacatg tatggctagc
    15901 tccaagttta gcaatcaagc aggtagcaaa catacgtgat cagctgattg aagcttaccc
    15961 agaaaaacag gaagtatgga caaaaaatgc agcagcctat accgagaaac ttcaagcact
    16021 ccatcagctt tatcaagaaa cctttaagca agcaaaacaa agatcttttg tcacgcaaca
    16081 tgctgcattc aactatctag cgttagaata tggattgaat caagtatcga ttgctggtct
    16141 ttcttctagt gaagaaccgt ctgcggcgag aattgctgaa ctgaaacact ttgttaaaga
    16201 acatggaatc aactatattt attttgaaga aaacgccaaa gatagtattg ccagaacgtt
    16261 agcaaacgaa gcaggtgtat ctttagaagt attgaatcct ttggaaggat tgacgaatga
    16321 acaaatagaa aatggagaaa actatctttc tatcatggaa gcaaacctcg aagcgttgaa
    16381 aaaaacgaca gagacagaaa acccgctaga agatactttg acccccaaaa aagaaaagag
    16441 cgtctacaac ggttattttg aagacgagga tgtacaagat cgctctcttt cagattgggc
    16501 aggaacttgg caaacagtag atacgtatgt agaagatggg acttttgatc ctgtttttga
    16561 atataaagaa aaattgaatc aggataagac agcacaagaa tataaagaat attatacgaa
    16621 gggctatcaa acagatattg cttcaatcaa gatcagtgag aaagagatgg catttacgtt
    16681 taagaatgga acaacgaaac gttcaaatta tcgttatgta gggaaagaaa tcttgaccta
    16741 cgaggcagga aaccgtggcg taaggtatct attcgaagca gaagataagg aaagtggcgc
    16801 ttttagatat attcaattca gtgatcacac gattgcaccg gctgactccg aacattttca
    16861 tttatatgca ggaaatgaaa gtcaagaagt attgctgaaa gaaatggaga attggccaac
    16921 attttatcca gatacaatgg atggacgtga aattgcacag gaaatgcttt tgcactgata
    16981 ggcaaaaaag aaccgaaggg tttggatcgg ctggtcattt agtcggtcca aactattcgg
    17041 ttctttttat gaaatcaatt tctttaatcc acggatagag gctgggatgg tctcgccgtt
    17101 tgccaacgtg acttctctgg tttttttatt aatcgaccgc acattttgtg tattgataac
    17161 ataggattta tgacaacgga taaaattccg atgcattttc tcgatttctt tgatcttctc
    17221 atagaattgt aattgggcat gagtcaaatg gacttccagt ttatgtggcg ctcctagcga
    17281 agtagaaaaa tattggatgt ctttgatcgg taaaaatttg atctcatcat taaactctat
    17341 tttgaagata tctttttgga tttgggacat ttgaaaatct tcgaaaattt tgctcaggat
    17401 atgctcgaca ttttctttta aatcataaag atcttctttg acgatataat cagtcggttc
    17461 gatgttgctt tttaagattt tcatcgacat ttccgtatgg gaagtcacat agattatttt
    17521 tcctaaagga tctaattcac ggatcaattt accgatcgct acaccatcca tttcactgtc
    17581 ttctagttcg atatctaaaa agtaaatacc aaaagaagaa gttctgatct tggctgcatt
    17641 gagaagttct tccggatcag aggtagaaat atggacataa ctgtctaagt tttcaatcat
    17701 gattctattt ttgatcaccg ttgataaaac ttctaattgt tttggatcgt cttcacaaat
    17761 ataaatggga atcatatcat tactccttta ttttatttgt atataaatcg tttgagaaaa
    17821 acgctggttt tcaattgatg tttcaaggaa aagataagga ttggtctttg tcaattcatc
    17881 caagataaac aagccaaggc cttcgttttt tggatttgtt tttgttgaga accctttttg
    17941 tttcaactgg tttaacggta atgtatttgt tgttgtattg atcacacgaa tcatcacgga
    18001 atgattcttc ttgagtatgg tcaattcgat ttttttcttc tttgcagcac tagctccttc
    18061 aatcgcattg tccaagataa taccaagcat acgtacaagg tttaccgtat gtttcgtatc
    18121 cagttcgatt ggctcaggaa aatgaagctg gacgatcaac cctttttcct gtgccatcat
    18181 gagttttaat gacagtaaac tacggatctc aggaactttc atattatcca acatacttag
    18241 attggccgga aaaaggttca tcatctggcg tgtaggtaat atcgtttgat cataataaga
    18301 ccgtaatcct tctaagtcgc cttctgaaat atagctgtga agagaaaaaa gcagattttg
    18361 gtaatcatgt ttgaattctc tcaagtcttg atagtttttt tctagatctt tgatataatc
    18421 ctgttgcatc ctgacttgtt gctctaataa ctgatcagct aatttcttat tagagatttt
    18481 ccaataataa accaagatga tcaggccaac acacagatat agaatgaaaa aactcgtatt
    18541 caaagctaag acatcaggtg tttcaccaga aaaacgggta taaaaaataa tgatatagta
    18601 tgtaaataat atcaatgtcc cgatcgtacc gatcatacgg cggtccacag tgtgaaacca
    18661 agtctctaac gattttttta aacaaaaagc taataaagca gataaaaaag cggataagac
    18721 aatgtgaaaa tactgcaatg aatctcctgg ttcaataacc gaagatttca aaacaaaaaa
    18781 atcgccgatg cttgttaagt gatctcctaa aagcgacgtc aataacccaa aaggaagcgc
    18841 gtaaaccaat tgttgacgcg aagtcgaaaa gaagaaaaaa gtaagcatca gcattcctat
    18901 agcaattaga gcagtaaaaa tccctaaaat cgaatagacc catccaatca aaatacaaga
    18961 aatcagaatg actcccgcaa acagatattt gtatcggatg tgatggtcaa tcaataagat
    19021 acaaattgtg taaatcaaat attgcaacgc tatggaccat aaaaacataa agtacagctc
    19081 ccattgattt ttaccaaatt atagcacaat tggcgtatta tttttttaat ttttcaggga
    19141 ttttgaattc agaactcata ccaaagcagc agccagatac aaaaaagtca gataagttag
    19201 ttaattgttt aatgattgtt tttttcatga aagattcctc caaaattcat caagtagttt
    19261 gtaataggaa gcaacgtagc tatttctaaa acaatcccaa gtagcaaata ggtagaaaag
    19321 taagaagctg agaaaaataa cgtaatgaga gaaaaaatta gaaacatgct gatcgccttc
    19381 tttaataatg ggggacgttt ctcgggttca atgggattga ttgctgtacc tcttgatcca
    19441 tagcgaagca gtaacaaaaa gcagctgatt aacaaaaaga ccatcattgg gaaggagata
    19501 gtcaattgca gatgtttcat caccaaggga acgccccata aataaagtag tgtccacagc
    19561 agacaaacga attcagagtc ggaatgcaat ccataagcat atctgcggat gcagatataa
    19621 gaaagaaagg tgattcctgt ttccgcccat gagcctgtca ccagagaaaa gactagaacg
    19681 accgctagtt ttccaagatt attcaataaa acttctaaga caaattttac ttggacatac
    19741 accatatctt cttccgattt gtctccgctg aataaggctt ctaataattt gttcacaact
    19801 ttatctttca tctcatacgt acctccacca caaattttat gctaaaaaaa ataaaaaacg
    19861 tcttttgttt cataaaaagt ggttttgaca tcaaaaccgt catgatctcc taaaaaaatc
    19921 ttcgttttcc aagaacctta ccaaaaaaag tgacagtttt gatacgaaaa gtacttttta
    19981 tgacaagttt gtgctttttc gagtgattta tgttaaaaaa ttactgacct tataaaaggt
    20041 gatggaggga ttcttatgga aaatgaacaa ttgtttgaag tcggcgaaac atatgcattt
    20101 aaatctgatt tatttgaatc accaataaaa ggaaaaatag aaaaaatcta tgaaaatagt
    20161 gtgatgatac gtgtcatgaa atgcgcagta gaagacaatg ataccgctca taatctgcac
    20221 tggcagttag ttgtacgcaa atcaagtaca atagaagaat aaaaacagat aaaatgttga
    20281 gagcataaag aggttgtgag tctcccgttt agctctgagt tacaaggaat aatgttaaaa
    20341 aacagctttt catgttttat gaatattgcc ttgtgaccga ggtgttggca ggagaatact
    20401 gtttatttga aaaaagggct gcgacaagac ttttgtcaca gcctttattt tcatgaatag
    20461 gagttacttt tggctacttt tagagaagta gcttttaagg agcagataaa gtcaaggctt
    20521 ttatttctat aaatgaagta gtaaaatgaa gaacagattt tattacagga aggatgccga
    20581 taattgtgat cattgttgca ggaacgatcg gtgcagggaa aagcacacta actgaaatgc
    20641 ttgcccaaga tttagaaaca aaaccttttt atgagaacgt ggaagataat gaagtattgc
    20701 cgctatttta ttctaatcca gaaaagtata cttttttatt acagattttc tttttgaaca
    20761 aacgcttttt ggcaatcaaa gatgctttca gccatgatga caacgtacta gaccgctcca
    20821 tctacgaaga tagtatgctg tttcatttga atgcagattt agggcgtgtt tctgaagtag
    20881 aagtaaagca gtatgaaggt cttttagaaa cgatgctgaa ggagttagaa gaaatttcgc
    20941 cacaaaagaa accagatcta ctggtttata ttcgtgtatc ctttgaaact atgttagcga
    21001 gaatcaaaaa aagaggccgg gaatatgaac agctagaaca agatcctgaa ctctattcct
    21061 actataaaga actcaatcga cgatacgaag agtggtatga acagtttgat atatgtccca
    21121 aaatagtgat cgatggggac aaatatgatt tcgtagccga tccggcatgt ggagaacaaa
    21181 tcgtacaaga aatcaagatg cgggcaaaga aaatgacaga agaggcagat gcccagtcga
    21241 tccgctacac tacaaataac ccgaaagcaa agggaatctt tacaactaaa taatataaaa
    21301 actaagcaaa ttgctcactc gcagatattt ctctgtgagc agcatcttgc ttagtttttt
    21361 tgattcattg agatcaaata gtgaaccgat tcgtatgata aaattgccat aaatgaaatg
    21421 tctgagtgag ggccatgatc aaaaataaaa tggaaacgat caaaatcgaa tgggcgccaa
    21481 acagatgatg acagaaaaca aggacaatag ctcctgcgat aaaggcaaga acgacactta
    21541 gatagtggaa aaatcgatca aaatctgctt tttctttaga aatacaataa tcatataagt
    21601 tcgttgctac cccttttaaa tttccagttg taaataaatt agtgtaggca cgcccgttga
    21661 ttttatcaaa agctacccac tggatcgatg cagtaaaagc aatcaaaatc gttactaatg
    21721 cgggatgctc atggacgggt gtcaaactaa caaagaaaaa aatcactgct tcataatata
    21781 gaacaaacaa gcgccagaaa tgtttttctt tttttctaaa ataatcaatt agatactttg
    21841 ttagaagaat ccctaagaaa aagaaaaggg tagacaatac ttttttacca acacttagcc
    21901 attctccgtc aaatgcttgg atgatcgata gaatgatatt gccagtttga gcagaggcaa
    21961 aagcaccata ttggatataa gtataactgt ccatggctcc tccgataaat gtcagcaaaa
    22021 gtcctattgt acgcccctcg tgaaaggaag tctgcatttt tttcatcacc ttttatgaat
    22081 atttttgtct tccttatctt agcataaacg attgaaaaaa aggaaaaatt ttcagaaaac
    22141 taaaatcata tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat
    22201 agacaacgaa actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga
    22261 aggaaatcaa tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct
    22321 gggagaacaa gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta
    22381 cctatgaagc ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag
    22441 aaagtgaagg aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca
    22501 atcaagaaca aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag
    22561 gagctgggag cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa
    22621 aacaagtaga cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac
    22681 taaaaaaacg agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc
    22741 atagtatctt ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc
    22801 aaggaataca aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac
    22861 agctgatacg taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt
    22921 ctcggttaga ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta
    22981 aagaaaaata tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg
    23041 gattgctcac ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg
    23101 tactccaaaa tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa
    23161 aatatgatta tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg
    23221 tgaaacggat gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga
    23281 gtatctatgg attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc
    23341 ctaccaaaac tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag
    23401 caaatagatt gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac
    23461 aaaaagaagc aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt
    23521 ggctaatcaa acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca
    23581 tattataccg atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata
    23641 ttccttttgt actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc
    23701 aacatttcta tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc
    23761 aggcctacca gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat
    23821 gcgatcaata cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga
    23881 taagccccaa tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta
    23941 aaacagacca gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta
    24001 tagctaagga aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg
    24061 ctactttcag aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa
    24121 aaacccgctt gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc
    24181 acaaaagtaa aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt
    24241 tgccttataa cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac
    24301 cagtaacgac gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa
    24361 atcgtctgta tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat
    24421 ttatcaaaga aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca
    24481 gaagtggtca gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt
    24541 gaaatttcag cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga
    24601 ggtgtgacaa gaattttgtc acacctccct tatttatttt gaaaatgaga atactattat
    24661 caaaatagag aatatataga aaaattctca ttattcaaac gtaaataatc agttattttg
    24721 atttttttta tattttatta gctaataatt ggtattttaa aagaaattac tgtagaattg
    24781 gattataatt aaaaaggagt gagaaaatga taaaagtata tacttcgcct agctgtactt
    24841 cctgtagaaa agcaagagca tggttgcaag cgaatcaact tgaatttgaa gagaagaata
    24901 tttttgccga accgttaaca gaaagtgaga tcaaaaagat cttggcagcg actgaaggag
    24961 gagttcaaga tattatttcc actcggtcga aaatttacga aaagctgggc atagatttta
    25021 acgaattaac tttaaagcaa atgatcgtct tattcaaaga atatccttca ttattgagac
    25081 gacctatttt gattgatgaa aaaagaatac taatcggatt taatgaagat gagatccggt
    25141 catttgtccc tcgtgaaatt cgatgtgttg ccagaaatct gatctattta caaaacaata
    25201 acatcttaaa ttcataggga gggagccttc gtggaagtga aagtctatgg atcagcgcac
    25261 tgtgtaacaa gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc ttatcaattt
    25321 gttgacttag aagaacgcga tctaagtaca gaagaagcag aagaattatg cagttttgaa
    25381 gaagtgcaag cagaacagct atttgcgact tggtctgagg cttttcacaa cattgcgatt
    25441 gacctttcct gtgtaggaaa aaaacaactg atttttttat gcacacaaca taccaatctc
    25501 ctcagacgtc ctttgattat tatcgatgaa gttctattta ttggatacaa tgagcagttg
    25561 atggaacagc ttatcttaaa taaatagact ttaactgttt taatcaagtc actgagcatt
    25621 tcatacagtt gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc tttttttgac
    25681 tttatttttc tactatagag acatgctcaa tttatgtggt caaaattcaa atctaggtgg
    25741 ggatatagag ttaaaaatat attgacatgt gtaaatttac atgatatatt catttctgtt
    25801 gttttaagta gcaaagcaat aaaatagtat tttaaaaaac atataaaata gttgttgaca
    25861 tgttagaaat gacatgataa gataactgag ttgttaaaaa agaaccactt gtgaagcttg
    25921 attctatcga aaaaaacttc aaaaaagttc ttgacaaata acaaatgata agttatgata
    25981 aatgagttgc tgtaagattt cgaaaaacga aatcaaaaaa acttttaaaa aagagttgac
    26041 aattacttgt ctctctgata tgatataaaa gttgctacgg cagccaaaaa cgaatcgggg
    26101 aacactcctg atgaagtaga cctttgaaaa ctgaacaaag taagacaaac caaatgtgta
    26161 gggcgtcttg attcaattca ag
//
LOCUS       DACECR010000033        26166 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.033, whole genome shotgun sequence.
ACCESSION   DACECR010000033 DACECR010000000
VERSION     DACECR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.033"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(130..825)
                     /locus_tag="IWO17_001268"
     CDS             complement(130..825)
                     /locus_tag="IWO17_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ0290154.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IWO17_001269"
     CDS             complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IWO17_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ0290155.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2024..2215)
                     /locus_tag="IWO17_001270"
     CDS             complement(2024..2215)
                     /locus_tag="IWO17_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ0290156.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2236..3126)
                     /locus_tag="IWO17_001271"
     CDS             complement(2236..3126)
                     /locus_tag="IWO17_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ0290157.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3113..3880)
                     /locus_tag="IWO17_001272"
     CDS             complement(3113..3880)
                     /locus_tag="IWO17_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0290158.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IWO17_001273"
     CDS             complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IWO17_001273"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ0290159.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IWO17_001274"
     CDS             complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IWO17_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ0290160.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IWO17_001275"
     CDS             complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IWO17_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ0290161.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8496..8834
                     /locus_tag="IWO17_001276"
     CDS             8496..8834
                     /locus_tag="IWO17_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290162.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(9002..9760)
                     /locus_tag="IWO17_001277"
     CDS             complement(9002..9760)
                     /locus_tag="IWO17_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0290163.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9778..10596)
                     /locus_tag="IWO17_001278"
     CDS             complement(9778..10596)
                     /locus_tag="IWO17_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0290164.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IWO17_001279"
     CDS             complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IWO17_001279"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ0290165.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IWO17_001280"
     CDS             complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IWO17_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ0290166.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11724..13058
                     /gene="dnaA"
                     /locus_tag="IWO17_001281"
     CDS             11724..13058
                     /gene="dnaA"
                     /locus_tag="IWO17_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ0290167.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13256..14386
                     /gene="dnaN"
                     /locus_tag="IWO17_001282"
     CDS             13256..14386
                     /gene="dnaN"
                     /locus_tag="IWO17_001282"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ0290168.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14615..14857
                     /gene="yaaA"
                     /locus_tag="IWO17_001283"
     CDS             14615..14857
                     /gene="yaaA"
                     /locus_tag="IWO17_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ0290169.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14844..15968
                     /gene="recF"
                     /locus_tag="IWO17_001284"
     CDS             14844..15968
                     /gene="recF"
                     /locus_tag="IWO17_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ0290170.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15965..17911
                     /gene="gyrB"
                     /locus_tag="IWO17_001285"
     CDS             15965..17911
                     /gene="gyrB"
                     /locus_tag="IWO17_001285"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ0290171.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17931..20402
                     /gene="gyrA"
                     /locus_tag="IWO17_001286"
     CDS             17931..20402
                     /gene="gyrA"
                     /locus_tag="IWO17_001286"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ0290172.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20638..20937
                     /locus_tag="IWO17_001287"
     CDS             20638..20937
                     /locus_tag="IWO17_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ0290173.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20987..21499
                     /gene="ssb"
                     /locus_tag="IWO17_001288"
     CDS             20987..21499
                     /gene="ssb"
                     /locus_tag="IWO17_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0290174.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21525..21761
                     /gene="rpsR"
                     /locus_tag="IWO17_001289"
     CDS             21525..21761
                     /gene="rpsR"
                     /locus_tag="IWO17_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ0290175.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21904..23877
                     /locus_tag="IWO17_001290"
     CDS             21904..23877
                     /locus_tag="IWO17_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ0290176.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23883..24335
                     /gene="rplI"
                     /locus_tag="IWO17_001291"
     CDS             23883..24335
                     /gene="rplI"
                     /locus_tag="IWO17_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ0290177.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24609..25976
                     /gene="dnaB"
                     /locus_tag="IWO17_001292"
     CDS             24609..25976
                     /gene="dnaB"
                     /locus_tag="IWO17_001292"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ0290178.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         7941 a         5155 c         5189 g         7881 t
ORIGIN      
        1 tttatcgtaa aaagggactg tgacaagact tttgtcacag tcccttacta ttatttaact
       61 atggataaac aaaatgaggt tctttactac ccctagatat ctagcaaaat agctgatctt
      121 tttgctagtt tatcttcttg ggacggataa acttccaata atcccgtatt ttttcttcaa
      181 gtagtaacgg attgctaaag cagttgcccc gataatgata actactactg gatctaatac
      241 tggattgata actggtggca atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt
      301 cgttccacct aggataagca atgtcttacc cattcctggt ttcttgcttc gatctgctcc
      361 gggatattca tattgataga tgtatttgta catcatataa aagacccagc caccgattgc
      421 agaacctaga acgagtgtag aaaagccata agctggcgtt gttccacgtg aaaacaatcc
      481 ggtaatcgca atcatgatac ttaatagacc aagcaacagc aacgtattat ctaaccacat
      541 caataaaggg gtggatgaat tttttacttc tgctggctta ttgatgattg cttccgttct
      601 ttcagagact gtcccaaaca attgacgggc tgttcttccg cctttttgtt ctgcaactaa
      661 agtaggcaga atgtcatgaa gggcttttac tttttcttct tcagacaaat tcgctgcttc
      721 taaagatttt ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt ttgtttctaa
      781 actattgttt tcttttacga ttgctcgtaa ttgttctggt tccattatct aatagtcctc
      841 cttagccgtt ccttttctca acggtcgtca caaaagacat tctaccatat tctacagcta
      901 gtttgatagc ccttttacac attaaagcgg aataacatta cgtcgccgtc ttgtacgaca
      961 taatcttttc cttctaatcg aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa
     1021 tgatccaagt cttcaaaaga gacagtttct gcacggataa atccgcgttc gaaatctgaa
     1081 tggatgattc ctgctgcttg tggtgctttg attccttttt tgaatgtcca agcacgtact
     1141 tcttgttccc cagcagtaaa gtaagtagct aagcctaaca aatcataagc agcacggatc
     1201 aactgatcca aacctgattc ttcgattcct aaagcttcta aaaattccgc tttgtcttct
     1261 tcatctaatt ctgcgatttc ttcttctgca cgagcactga ccacgatcac ttctgcattt
     1321 tcttctgcag caaagttgcg cacttcttgt acataatgat tcgcgtctgc atctgctacg
     1381 tcatcttctg agacattggc tacataaaga attggttttg tcgtcaataa aaatagtgat
     1441 ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc gggcagattt tccttcttcc
     1501 agtacaggtt tgattttatc cagtacagct agttctgcca ctgcatcttt atctttggtt
     1561 ttagcaactt ttgctacacg agtatagcgt ttgttgatag attctaagtc tgctaacact
     1621 agttccaagt tgatcgtatc aatatcttct agtggatcaa cacgtccttc tacgtgagta
     1681 atattatcat catcaaaaca gcgaacaacg tgacaaatag cgtctacttg gcggatatgg
     1741 cttaagaatt gatttcccaa accttctcct ttacttgcgc cttttacgat tccagcaata
     1801 tctgtaaatt caaaagtcgt cggaacagtt tttttcggat gtacaagttc tgttaagcgt
     1861 tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga
     1921 tagtttgctg cttctgctcc tgcttttgtg atcgcattaa acaaagttga ttttcctacg
     1981 ttgggcaacc caacaattcc agcagtcaat gccataaatt tattcactct ttctctgcat
     2041 ttttttcaat tacttttttt aatttctttt caaaatctcg acgggtcatc atgacgatat
     2101 gtccgcaatt catacatctg attttgatat ctgcgcccat tcgaatgact tcccagcgat
     2161 tcgtctggca agcgtgaggt tttttcattt caacgatatc gtgcaagtcg tacataaaaa
     2221 tagcctcctt tttgtttatt cttcatcaaa ttgaatattc aagatatcaa gaattcgagt
     2281 caaatctttt tgagaaagat attcaatctc gatcttacct ttcccatttt tttcttggat
     2341 agctacactt gtaccaaatt tatccatcaa acgatcttcg ctttctcgga tataatatgg
     2401 tttttccttt gctagccttg ggattttttt cttttctttt ccatcatttt gatttaactc
     2461 agaaacaagc tgttccagtt ggcggacagt taaattttca gttactgcac gattggctaa
     2521 tttcaagatc aatgttttat tttttaaacc taacagcgta cgggcttgtc ccatagataa
     2581 acgctgatct tgtaccattt ccttgacaag atccggtaaa gaaagcaagc gcaaataatt
     2641 ggcgatataa ggtctgctct ttcctaaacg actagctact tctgcttgtg ttagtttcag
     2701 gtttttcatc agcatctcat atgcttctgc ttcttctaac ggattcagat cttctcgctg
     2761 caagttttcc agcactgcta cttgcatcat cgcttcttcg tcaaagtctc taacgatagc
     2821 agggatcgtt tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat
     2881 ctcatatcct tttactgcgg aacggcgtac gatgatgggt tgaaagacac ctgattgttc
     2941 aatagaatta gctagttcct gcaaagatac ttcttcaaat gttttacggg gctgatacgg
     3001 atttggtcgc agctcattca atggtagctg gacaacggta tcatctttta catccaccgt
     3061 ctctaagttt tctaaatctt gaaacagtgc atcgattcct cggcctaatc ctttactttt
     3121 cttcacgact taccacttcc tttgccaatg cttggtagac ttcagctcct cgagaacgtg
     3181 gatcgtagtc gatgatcggt tttccatgac taggtgcttc tgataaacga atatttcttg
     3241 gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg tacttcttct acgacttcat
     3301 ttcccagatt tgttcgtgca tcatacattg tcagtaagac tccctcgatc tctaattctg
     3361 gattaaagtg tttttggact aaacgaaccg tattcagtaa ttggcttaat ccttccaatg
     3421 cgtaatactc acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg
     3481 tcagatggcc aagagaaggc gggcaatcga tcaaaatata atcatactga ctgcttactt
     3541 ctgctaaaga tccttttaat cttgattctc ttgccatcat tgacgtcaat tcgatctctg
     3601 ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag
     3661 ttgcctcatc aattggcaat tcattgacta gaacatcata gatgtctctt gtcacatcag
     3721 gttttctgat cccgacacca ctggtagcat ttccttgtgc atccatatcc actagcaata
     3781 cttttttgcc aaggctagca agacaagcac ctagattcac cgtcgtcgtt gttttgccga
     3841 cgccaccttt ttggttcgca acagatatta tttgtgccat ctcttctcct cctatttgat
     3901 tggctgctta ttaggcagtc cgggctttct tgggtatttc tttggggttg gtttcttttt
     3961 ttgaataacg atgatatgcc gttgatcaga gacattcggc aaggtaaccg cctcttcttt
     4021 gaggaattta ccacctaata aagcaatcgc ttgttttgct tctgtcaatt cctcttcact
     4081 ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta gccaagggca aacacaattc
     4141 cgctaacaca ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa
     4201 gtgaggattt tgcccaaatg tttctgcccg gtcatgatat aaggaagcat cgattcctaa
     4261 taatcgggtt aactcagata agaaggttat tcgtttattc agagaatcaa cgatcgttat
     4321 ctgcagttct ggaaaggcaa tttttaaagg tatgctagga aaccctgccc ccgaaccaac
     4381 atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta
     4441 aaaatgcttc agatatacgt ctttttcatc cgtaatagct gtaaggttca tcttttcgtt
     4501 ccattcgact aatgtccgat aataaatagc gaattgattc atttgatgat cggtaagctc
     4561 tatccctttt tctgccaagg attggcgaaa ttcttctggt atcataaata ttcctttcat
     4621 tgtgaaacag caattgtttc acagattctc tccctacttt aacgcaaaat ctctagacat
     4681 gcaatccttc catatggatt cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt
     4741 ctttctagta tgactcataa aatatacaca gaagagagaa ctagctttga gcaatttctg
     4801 caaaaactag ttctctcttc tatttattat tacgtattac gctggttcac tgactttcgc
     4861 aatcttccct tgttcgatat agaccattag gatacttagg tctgcagggt ttacgccact
     4921 gattcggcta gcttgggcaa ttgtttctgg ttggattttc tgcaattttt gttttgcttc
     4981 tgttgctaat ccgttgattg ctgcgtagtc gatgttttct ggaatctttt tcgcttccat
     5041 gcgttttaat ttttctactt tttccatggc ttttttgatg tagccttcat atttgatttg
     5101 gatttctact tgttcgattt cttttcgtgt caattcttca ttttctggaa taaaccgtag
     5161 aagttctgca taagtaatct caggacgtct taaaaagtcg cttgctagta caccatcttt
     5221 taatggtgcc gcattaatcg ttgctaaaaa gtcttggact tcttctgttg gtttgattcg
     5281 tacattcatc aaacgtttga tttcttgttc aactgcttct tttttcttca aataattctt
     5341 gtattgatct tcttttacta agcctacacg gtgacccatt tctgttaatc gcaaatctgc
     5401 attatcatga cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac gataaggttc
     5461 gttcgttcct tttgttacca aatcatcgat catgacaccg atgtaaccgt cacttcgttt
     5521 gatcactaaa ggttcttttc cctgaacttt caaagctgca ttgataccgg caatcagccc
     5581 ttgtccagcc gcttcttcat aaccgcttgt accatttgtt tgtccagctg tataaagatt
     5641 ttcaacgact ttagtttcta atgtcggacg gagctggtgt ggctctacta cgtcatattc
     5701 gatagcatat cctgtacgca tcatttctac tttttctaat ccagcaatag aatgaagcat
     5761 gttgacttga acatcctctg gtaaagaagt agataaacct tgaacataga cttcttccgt
     5821 atttaagcct tctggttcca aaaataattg atgacgtggt ttgtctgcaa aacgcacgat
     5881 cttatcttcg atggatggac aatagcgagc gcctacccct tcaacgatcc ctgtaaacat
     5941 tggggcacga tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg tataggtcag
     6001 ccagcaagat tcctgatcta gtttataggc actatctggt gtgctgaagc taaaatgatt
     6061 cggctctttg tcaccaggct gctcttctgt aacagaatag tcgatcgtac ttgattttac
     6121 tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag ccaagttctt ttagatgatt
     6181 cgctagtcca atagaaggtt gagagttatt cggacctgat gaatatttca attcaccgat
     6241 aatgatttct ccgcgaagtg ctgttccggc tgtaataaca actgctttgg cccggtagct
     6301 cgctcctgtg gacgtcacta ctccatgaca aacaccatct tcgacgatca gttcttcgac
     6361 gattccttgt cttagcgtca aatttggttc tttttcgatc gtatgtttca tttctgttga
     6421 ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt
     6481 caacatacgc atttggatat aggttttatc gatattctta cccatttctc cgcctaacgc
     6541 atcgatttct cgaacgacaa cgcctttagc tggtccgcct acagaaggat tacatggcat
     6601 gaatgctacc atgtccaaat ttattgtcag taataatgtt tttgatccca tacgtgcagc
     6661 agcaagtgca gcttctgaac cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc
     6721 tgcttcataa tgttccaatc gtttgttcct ccttttattt tcctaaacaa aattgactga
     6781 ataattgagt gattagctca tcttgtacac tgtcgccgac aacttctcct agatagtccc
     6841 agcaacgcgt catatctatt tgaacgagat ccacaggcat tcctgaatca atacctgcaa
     6901 tgacttcttc aagtgagagg gaagcttttt ctaacaacgc gatatggcgg gtattagaaa
     6961 cataagtagc gtctctttct cctgtttctc cgctgaagaa taaatcagaa atagctgatt
     7021 ccaaacgatc tagtccgtcg tttttagcta cggaaattga aaaaactggt tcattttcga
     7081 tcaatttctt caatttttct tgttccagct gtgctggcaa atctgtttta tttagaagaa
     7141 tgatccgttt caatccactt gtcgcttcca ataattgttc atcttctgct gtcagtggtt
     7201 cgctttggtt tagaacaagt aagatcaagt ctgcttctgc taatgctttt cggcttcttt
     7261 cgaccccgat ccgttccacc acatcttctg tttcccgaat tccagctgta tcgattagct
     7321 tgagcggtac cccccgaaca ttgacatact cttcgattac atctcgagtc gttcctgcaa
     7381 tatccgtaac gattgctttt tcttctctta acaaatggtt taacaaactt gatttgccga
     7441 catttggccg gccgatgatt gccgtactta acccttcccg cagaaccttc ccttgcttgg
     7501 atgtagcaag tagcgcttgg attctttgtt ggatcatctg cgctttttca agcaataact
     7561 tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc aatatttact tctacttgtg
     7621 ctaatgtctc taaaatctct tgtctcaatg agcggatcaa agcagacaga ttgccgtcta
     7681 actgattaag agctagtccc attgctttgt ctgtttttgc ccgaatcaaa tccatcacag
     7741 cttctgcctg tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg aattcgcctg
     7801 gctcagctaa tcttgcacct tctcttaaca atagctgcaa aatttgattg actacaacga
     7861 tcccaccgtg acaattgatt tctactacat cttcccgcgt aaatgtcttt ggtgctctca
     7921 tcacggagac catgacttca tcgacaagct gattgctctt gggatcaacg atatgtccat
     7981 aatggatcgt atgacttggt acttctgata atcgtttgtt tccagattga taaactttat
     8041 ctgctagttg cacagcttga tcgccactta aacgaacgat actgattgca ccttctccag
     8101 gtggggtaga aattgccgca atcgtatcaa attctaaagt aatttcagcc attttttcac
     8161 ttcctttttc attttgcgca caaaaaaagt gcccactcca ccctttatgc acaataaacg
     8221 gtgaacttcg cactttgact gcttgttctt gattaaattc tggtcataga ttatcacact
     8281 tgctataagg aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat
     8341 taccttatat ttaattgtcc ttggatgtct ctttgcgttc tttggtttat tcgaagagaa
     8401 gatactgtat aaacaaggct ttatctttct taaaaaaaac ggtacactaa atggtagcgc
     8461 tatctaaact atacactttt gaaaggagaa aatatgtgag cccgttcaga tctgctgcat
     8521 tggaagaaga aagtgaccct cggatcaacg atgtcgtttt atctacaggg aatatcaacc
     8581 agaaatatat cgtccgcgat atcgtttttg ttgtggaatg cttagaagct gatttgtttg
     8641 acccatcttt tcccaaagat gaccttttct taacaacgaa gaggaaatta aagacgaagg
     8701 tacttgaata tggtgcgaat gcagtaatca actgtcattt tgagtatcag caaaaaaaac
     8761 gcgacggaaa gtattttctg gaactttttg cttatggaac agtgatccaa tttatccaaa
     8821 caacgattgg ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga cagtttcgat
     8881 ccgctttttt ctatcaaagt tagtgactac ttcatcgtat actctgataa gaatgatttc
     8941 tagtaaaata tacttggttt gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga
     9001 atcaataata ttttttagca ggttctacta ctaaataacg ataaggttcc tcaccttccg
     9061 aatgtgtttt tacatgttca tctttgctta atgcagcatg gatttgttta cgttcaaatg
     9121 caggcattgg ttctaagaaa acaggctgcc ctgtccgttt tactttttca gccgtgcgat
     9181 cagctaaacg ctgaatgatc tcttggcgct tttcacggta attgcctaca ttgactacta
     9241 cagacagctt atttcttgct aaacgatgga tgaagacttg tgccagatat tgcaatgcat
     9301 tcagtgtttt tccatgtttc ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa
     9361 caaccatatt atttgattct ctttccattt tgacaagagc tggagcatca agctgttttg
     9421 aaatttcagt aagataaact gccaattccg ttaaagctgc ttcatcacct agctcactta
     9481 cttcttctgt tttttcttca agaatcgttt cgttgactac ctcgatctca gctgttggtt
     9541 cttcttccaa aatttcttct acagtctgtt ctactgtttc agaaactttt tcgctgatac
     9601 ttggctggat cgaaagccgt gcattctttc gacccattcc taggaaacct ttttttcctt
     9661 cttccagtat ttcgatttcg acttggtctt tggccaaacc caaatcagcc agacctagtt
     9721 ggacagcttc gtctattgtt gcagcttcat aaatcggcat tagcattacc tcccctatta
     9781 tttccgtttt ttcttaggat tcattgcttt cttaagagca cgttctcttt cgcggattct
     9841 ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg
     9901 tacaacttgg aaggcattgg aaactaccca gtataatgaa agaccgctgg ctaaattgac
     9961 acccatcact aaaatcatta caggcatcac gaaattcatg atcttcattg atgcattcga
    10021 ttcaatttga ctcatgctag ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat
    10081 cggcaaaata aatgttggat ccgcgttccc taagttcaac cataagaaat gcccttgttt
    10141 caactctggt acacgtgaaa tagattgcca taacgccatc aagattggca tttgtactaa
    10201 taaaggaaga caaccagcat atggattgac gccattttct gcatataaac gctgctgctc
    10261 ttctcttaac ttactttgtg tttccggatc tcttgaacta tattgttgct gcagtgcttt
    10321 taatttcggt tgtaattctt gcgttttacg catgctcttt gtttggaaat gcatcaacgg
    10381 caataggatc acgcggatga tcagtgtaaa taaaatgatc cctatccctg cattaccaaa
    10441 tgacaacgcc ttgatcgcct cagcaaagta ataaacaata taacgatccc aaattcctgt
    10501 actctgtgcg ctgacttccc cagttccgca accggataag acgatgacca aactaacgat
    10561 accggccatc aaaagtaaac gtttgtattt cttcacttac tcttttcctc tctgtcgatt
    10621 atttttgcaa gttttaagac gtggactaaa ttggagcgaa cctcatcata agtcaacccc
    10681 ttcacacttg gtctagcaat cacaataaaa tccaactccg gatcaatttc ttcttttata
    10741 gaataaactg ctgctcggat ttttcgtttg acttcattgc ggtttaccgc gttgccgacc
    10801 ttttttccta cagataaacc aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa
    10861 acgacgaatt tgcggttagc aaaagaatct cccgtatgga acacggcttg aaactctcgt
    10921 tcttttttta ctcgatacgc ttttctcatt agttttcttt ccttttctat ctctcactta
    10981 cgctcataat cataccataa ttacgattgt cctttcatca tttccctaaa aaagagtata
    11041 aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc tttgcgacga
    11101 cggctagcta acacacgacg tccattttta gtactcatac gtttacggaa tccgtgaact
    11161 ttttgacgtt tacgtttatt tggttggtat gttcttttca tatattccac ctccaaaatg
    11221 ttcttatcta ttttataaac acagacatac tttgatagta taacgacaaa gtagtgattt
    11281 tgtcaatata aaatagaaga tcgattgtta aaacttatcc acaagattat ttttttctat
    11341 gggaatccta tgtggaaaaa gttatgcaca gggattatcc acagataata aaaggctggt
    11401 tttacacaga aaacacacgt gtaaaaaaag taatccacaa tcaggcgatt tcctgtgtat
    11461 tccgtctcct aactattggt atatctgtat tttttatcca cagacgcaag aggaaatcta
    11521 ttggttttta cgagttttcc acctgtgcat tagtggtgtg gaaaactttt tttgacaact
    11581 gtgtttttct cttttttctt tttgtcgatt gtgtggataa ttgtaattaa aaaatttttt
    11641 tcttcggttt ctgtggaaaa ctattgaaaa aaatgctaaa ttagtactag ttattcactt
    11701 tttaatgaag gaggaggcct tatatggtat ccctcgatgc tttatggaat gaattaaaag
    11761 caacatacca aaaggattta tcgcctgcta gctataatac atggatcgaa acagcgaacc
    11821 ctcgaaccct tgatcaaaac cagctcgttg ttgaggttcc tagcaagatt cataaagaat
    11881 attgggaaaa aaacctggcg acaaaaatcg ttgaagtcgg ttatatgtta tcaggaaatg
    11941 agattatccc tcgctttatt actggcgaag aagcagagca agaagaagtt atagaagaaa
    12001 aaaatccaaa agttgtggca cctagtccgc taaaaaaagc catgctgaac cctaaatata
    12061 cctttgatac gtttgtcatt ggtaaaggaa accagatggc tcatgcagca gcgctcgttg
    12121 tagcagaaga tccaggttct atttataacc cgctcttctt ttatggaggc gtgggacttg
    12181 gtaagactca cttgatgcat gcaattggtc atcaaatgct gcaaagtcag cctaatgcca
    12241 aagtaaaata tgtaagcagt gaaacttttg ctaatgattt catcaattcg atccaaaaca
    12301 aaacagcaga agagttccgt caagaatatc gaaatgttga tttactgcta gtggatgaca
    12361 ttcaattttt tgcagaaaaa gaagctacgc aagaagagtt tttccatact ttcaatgcct
    12421 tgtacaatga agggaaacaa atcgtactaa ccagtgatcg gttgccaaac gagattccga
    12481 aactgcaaga acgccttgtt tctcgctttg catgggggct gtctgttgat attacaccgc
    12541 cggatcttga aacaagaaca gcgattttac gtaaaaaagc ggcggctgaa cgtctagaga
    12601 tcccagatga cacactaagc tacatcgcag gacaaattga ttctaacatt cgtgaattag
    12661 aaggtgcgct tgttcgagtg caagcttttg ctgcgatgaa tagtgaagat atttcgacta
    12721 gtctagctgc agacgcactg aaaacgttaa aatcaggaaa aggacatcct caattgtcaa
    12781 tcctgcagat ccaagaagaa gtggcaaagt attatcacat ccaattgaaa gatctcaaag
    12841 gaaagaaacg cgtcaaatct attgttgttc ctagacaaat cgctatgtat ttggcacgtg
    12901 agctcacaga taattcttta ccgaaaatcg gagctgaatt cggtggcaag gatcatacaa
    12961 cggttatcca tgctcatgaa aaaatccagc agctgatgga ttctagtgtc agtatgcaaa
    13021 atgaagtatc agaaatcaaa aactttttac taaattaatt tgtcgattct atcaactgaa
    13081 aatagcatgt ggataaaaga aaaaaacgtt caaaagttat acacactttt tccacagctg
    13141 ttttttcgtt tctctcttac gttttttgag ttttccacag aatcaacatg ccctattact
    13201 tttattattc tttttaacta ataataaata aacaaacatt caaaggagat acatcatgaa
    13261 agttacttta aaccgagcta gctttatgca ggaattgcaa actgttcaac gagctatttc
    13321 aagcaaaacc acgatcccta ttttgacagg tgtaaaaatc acactgacac aagaaggttt
    13381 gactttgacg gggagcaacg ctgatatatc aattgaaact tttttgtctg ttgaaaacga
    13441 aaaagcaaat atgcaaatcg aatctactgg ttccattgtt ttacaagcac gtttctttag
    13501 cgaaatcatt cggagacttc ctgaagaaac atttacttta gaagttttag aaaataaaca
    13561 agtagcgatc acttctggaa aagcgaattt tatcgtaaat ggattagatg cagataacta
    13621 tcctcatctt cctgttgtcg aaagccataa ccagatgaaa ttacctgtac acgtattgac
    13681 taaactaatc aacgaaacag tttttgctgt ctcacaacat gagagtcgtc caatcttgac
    13741 aggtgtgcat ttcattttat ctgataattc tttattagct gtagctactg attctcaccg
    13801 tctaagtcaa cgcgtgattc cagtagaaca agcggctgat cattttgata ttgttattcc
    13861 tggaaaaagt ttgatcgaat tatctcgctc attaacaaat gaagaagaaa tcgtcgaaat
    13921 cagcattatg gaaaaccaag tgttgttcaa aacagaaacg atgtatttct attcccgttt
    13981 gctagaagga aactatccag ataccaatcg tttgattcct tcaagtttta atacagaggt
    14041 tgaattttct gttcctagct ttttagcggc gatcgaacgg gcttctttac tttctcatga
    14101 aggccgtaac aacatcgttc gtttatctat tcgtccagat gcggttgttt tatacggaaa
    14161 ttcgccagaa atcgggaaag tagaagaaag cttgagttat acagcaagca gtggcgatcc
    14221 attagatatt tctttcaatc cggattatat gaaagcagcg ttgcgtgcat ttggagatat
    14281 gagcatcaaa gtgaaattta tctctgctat tcgtccattt acattggaac caacggaaga
    14341 tggcgttcaa tttattcagc tgattactcc tgtacgtaca aattaacaat gattttcaat
    14401 gttcttgaaa atttaattga aaagaaatag aaaaaataaa attttaaaca gaaggcccta
    14461 aaatctcttt taaggctttc tgtttatttt tgtttaaatc acctttttta gattaaatcg
    14521 tcttatatgg aaaaatatat ggataaattt gttttattgc gaaaaaaaag gtataataga
    14581 gtatactttt aataatgaaa atgagggatg ggcattgaaa caacaagtca ttctaaatac
    14641 ggaattcatg acgttgggcc aaatgttgaa agaagtcaca gttatcggta gtggcggaca
    14701 agcaaaatgg tatttggcag aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg
    14761 tggacgcaaa ctttatcccg gcatgatggt tgaagttcca gaagtaggaa ctttttttat
    14821 ggtaaaaaaa ggaaacgacg ataatgaggc tgaataagct gtatttaaaa aattatcgaa
    14881 attacgaaga gctgaacatc gaattttcca agaacctcgt catcttttta ggagaaaatg
    14941 cgcaaggaaa aacgaatctt ttggaaagta tctacgtatt agctatgacg cgaagtcatc
    15001 ggacaagtag tgagcaggaa ttgattggct ggactgacga tcaggcgatg attcaaggag
    15061 agatcacaaa aggttcatcc actcttccat tagaaatctt attatctaaa aaaggccgaa
    15121 aaaccaagat caaccatatc gaacagaaaa agttaagtac ttacgtggga caaatgaatg
    15181 tcattttatt tgcaccagaa gatctttctt tagtaaaagg cagcccgcag atcaggcgga
    15241 agtttttaga tatggaatta ggccagatca atccaatcta tctttatgat cttgtccaat
    15301 accaagcaat tttgaaacaa agaaaccagt acttgaagca attgaatgaa aaaaaacaaa
    15361 ctgatttgct ttatttggat attttgtccg aacaactagc agcgtttggc agcaaagtat
    15421 tgaaagcaag agcacagttc gtcaagcgat tggagttttg ggctaattct ttgcatcagc
    15481 aaatcaccca tcaaaaagag cagctggaaa tcgagtatct caccgcagtc gattctttag
    15541 aaacacatac gcaagagcaa atccaagaac aatttctcgc acttttgaac caaaataaaa
    15601 agaaagacct ttttcgggga acaacgacag ttggtcctca tcgtgacgat ctttcctttt
    15661 ttattaatca gaaaaatgtg cagacttatg gatcgcaagg acagcaaaga acaactgcac
    15721 taagtgtcaa attagcagaa atcgatttga ttaaagaaga gacaggggaa tatccaatcc
    15781 ttttgttaga tgatgtgatg agcgagctgg atgataatcg tcaactccat ctgctagaaa
    15841 caatcgaagg aaaagttcaa acgttcttaa caacaacgac acttgaccat gtaaaaaata
    15901 aaatgactgt tgaacctgag attttttatg ttcaacaagg gaaactaaaa ggagaagaaa
    15961 ctgaatgaca gaagaaagaa gtttagtaga acgcgctaaa gagtatgatg ccagtcagat
    16021 tcaagtactt gaaggacttg aagccgttcg taaacgacct ggcatgtata tcggttcgac
    16081 tagttctgag ggtctgcatc accttgtttg ggaaattgtc gataattcga tcgatgaagt
    16141 attagcaggt tttgcaacga aaatccatgt gattattgaa aaagacaaca gtatcaccgt
    16201 tattgacgac ggtcggggga tacctgtaga tatccaggca aaaactggac gtcctgcagt
    16261 tgaaacggtc tttactgttt tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt
    16321 ttctggtgga cttcacggag taggatcttc tgttgtaaac gcgctttcta cacagcttga
    16381 tgtcaaagta tacaaagaag gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt
    16441 ggacgactta aagattatcg aacaaacgga tcgccatgga acaacggttc actttacgcc
    16501 agaccctgaa atttttacag agacaactga gtttgatttt tcaaaattgg ctacccggat
    16561 ccgtgaactt gctttcttga accgcgggat gcgtatatcg attgaagaca aacgagaaga
    16621 agaaccagtc atcaatgaat accattatga cggcgggatc aaaagttatg tcgaatactt
    16681 gaatgcaaac aaaaccgtga tttttccaga acctgtatat ttggaaggtg aacagcaaga
    16741 tatcgcagtt gaagtctcta tgcaatatac agacggctac cattccaata tcatgagttt
    16801 tgccaacaat atccatacgt atgaaggtgg aacacatgaa tcaggattca agactgcttt
    16861 aactcgtgtg ataaatgatt atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa
    16921 cttgaccggt gaagacgtgc gagaaggatt gacagctgtt atttctatca agcatcctga
    16981 ccctcaattc gaaggacaaa caaaaacaaa attgggcaat tccgaagtac ggacagtcac
    17041 tgatcggtta tttagcgaac atttcatgaa attcttgctg gaaaacccaa gtgtgggccg
    17101 gcaaattgtt gaaaaaggat tactagcttc cagagctcgt ctagcagcga aacgtgcgcg
    17161 tgaagtaacg agacgtaaag gtgctttaga gattagtaat ctgccaggaa aattagcgga
    17221 ctgctctagc aacgatcctg aaaaatgcga attgttcatc gtcgaaggag attcggccgg
    17281 cggatcagct aagcaaggtc gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa
    17341 aatcttgaat gttgaaaaag caagcatgga caagatcttg gcaaatgaag agatccggtc
    17401 tttatttact gctatgggta cagggtttgg agaagatttt gatgtatcga aagctcgcta
    17461 ccacaaatta gttatcatga ctgatgccga tgtcgacggc gcgcatatcc gtacattgct
    17521 gctgactctt ttttatcgtt acatgcgtcc tattgttgaa gctggatacg tctatattgc
    17581 tcagccacct ttatacgggg taaaacaagg gaagaatatt acgtatgtac aaccaggaaa
    17641 aaatgcagaa gaagagttga aacaagtcgt tgcttcactt cctgcctcac caaaaccaag
    17701 cgttcagcgt tataaaggac ttggagaaat ggatgatcat caactatggg aaacaactat
    17761 ggacccaagc aatcggatga tggcccgcgt cagtgtggac gatgctattg cagcagatca
    17821 gatatttgaa atgttgatgg gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa
    17881 tgcacactat gtgaagaact tagatattta atcatgaagg aggaaaaaga atgagtgaag
    17941 aaatcagaga aaacatccat gatgtcaatc taaccagcga aatgaaagac tccttcatcg
    18001 attatgcgat gagtgttatt gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac
    18061 ccgttcatcg ccgtatttta tacggcatga acgaattagg tgtaacaccg gataaagccc
    18121 ataaaaaatc tgccagaatc gtcggagatg tcatgggtaa ataccatcct catggagaca
    18181 ttgcgatcta tgagtcaatg gtacggatgg cacagccatt cagctaccgt tatatgctag
    18241 tggatggtca tggaaacttt ggttctgttg acggtgacgg tgccgccgct atgcgttata
    18301 ccgaagcacg gatgagcaaa atcgctacag aaatgctgcg tgatatcaat aagaacactg
    18361 ttgatttcca aagcaactat gatgatacag aaaaagaacc agttgtacta cctgctcgct
    18421 tcccgaatct tttagtgaac ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc
    18481 cacctcataa cttgtctgag gtagtagcgg ctatcgattt gctgatggat aatcctgatg
    18541 tgacgaccaa tgaattgatg gaagtattac ctggaccaga ttttccaaca ggtggtttgg
    18601 ttatggggaa atcaggaatc cgaagagcgt atgaaacagg taagggttct attaccgtac
    18661 gtgcaaaagt tgagattact gagatgccaa atggtaaaga acggatcatc gtgacagaat
    18721 tgccttatat ggtcaataaa gcaaaattga tcgaacggat ttctgaactg cacagagaca
    18781 agcgtatcga agggatcaca gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg
    18841 tcattgacat ccgtcgtgac gccagcgcat ccgttatctt gaataactta tataaattga
    18901 catccttgca aaattcattt ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag
    18961 ttttgagctt gaaacaaatc cttgaaaact atatcgaaca tcaacgcgaa gtcatcacac
    19021 gacggacggt ctttgaaaaa gaaaaagcag aagcaagagc acacatctta gaaggtttgc
    19081 gtattgcttt ggatcatatc gatgaaatca ttgcgattat ccgcgggtct aaatcagatg
    19141 acgaggcaaa agcaacaatg atcgaacggt tcgatttatc cgatcgccag tctcaagcta
    19201 ttttagatat gcgtttacgc cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat
    19261 accaagaact cttaaaactg attgaagatt taactgacat tctggcaaga ccagaacgtg
    19321 ttacagaaat catcaagaat gaattagcag acttgaatca acgatttggt gataaacgac
    19381 gcacagaatt actagtcggt gaggtcttaa gtctggaaga tgaagattta atcgaagaag
    19441 aagaaatcgt tattacattg acaaacaatg gttacatcaa acgtttggca aataatgaat
    19501 tcagagctca acgaaggggt ggacgtggtg tccaaggaat gggtatccac aatgacgact
    19561 tcgtcaaaaa tctggtttct tgttctaccc atgatacatt gttattcttt acgaataacg
    19621 gaaaagtgta tcgggcaaaa ggatacgaaa ttccagaata cgggcgtaca gccaaaggaa
    19681 tcccaatcat caatttacta ggtatcgatt cgagtgaaag catccaggca atcattgcgg
    19741 tgacgggaga agcagaagaa ggccactatc tcttctttac gacgcgtcaa ggtacagtaa
    19801 aacgtacttc tgtcactgct tttgctaaca tccgaagtaa tggtctgatc gcaattggtt
    19861 tgaaagaaga cgatgagctc gtcaatgtgc tactgacaga tggtcaatct aatatcatta
    19921 ttggaacaca taacggctac tctgtcactt tcaaagaaga agcagtccgc gatatgggac
    19981 gtacagcttc cggggtcaga gggatacgcc tgcgtgagga agattatgtc gttggggcag
    20041 ccttaatgaa ggaaaatcaa gaagtattag tgatcacaga aaaaggttac ggaaaacgga
    20101 caaaagtatc ggaatatcca gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca
    20161 tcactgaaaa aaatggtcca ctagctggat tgaccactgt ttctggagat gaagacatca
    20221 tgctgattac agataaaggt gtcattatcc gtttcaacgt atcgactgtt tctcaaactg
    20281 gacgttcaac attaggcgta cgtttgatga agatggaagc tgatacgaaa gttgtcacga
    20341 tggcagcagt cgaaccagaa gctgcagaac aaacagttga agaacaagga tcagaagaat
    20401 aaccatcaag actaagtgtg taagctagca agaataaccg attaaaaaat ctggttattc
    20461 ctgctggctt ttttgattag ttgaatttct cttaaaatat tcgaaactgt tcattctgta
    20521 ttgcttttga ccaaaataga gtgtataatt agagcttgtg agtagtctaa ttaattagat
    20581 aactctcctt gctcttagga acctaagagc cgaaagtcca cagggaggtg aatatcaatg
    20641 gaaaacacga aatatgaaat catgtacatt attcgtccaa acattgatga agaagcaaaa
    20701 actgctttaa tcgaacgttt cgatgcaatc ttgaaagata acggagcaga agttatcgaa
    20761 tcaaaagatt gggaaaaacg ccgtttcgct tatgaaatga acggttaccg tgaaggtatc
    20821 tatcacatcg ttaaagtatc ttctccatca tcagcaaatg ctgtcaatga atttgaccgt
    20881 cttgctaaaa tcaacgacga tatcattcgt cacatgattg ttaaagaaga agcttaattg
    20941 ttgtttcatg tgaaacaata gaaaacgatg agaggagatg actattttga ttaataatgt
    21001 tgtattagtt ggacgtctga cgaaagatcc agatttgcgt tacacagcaa gtggtacggc
    21061 agtcgcaaca tttactttgg ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga
    21121 ggctgatttt atcaactgtg tgatttggcg caaatcagca gaaactttag caaactatgc
    21181 tcgtaaagga acacttttag gtgttactgg acgtatccaa actcgttctt atgataatca
    21241 gcaaggacaa cgtgtatacg tgacagaagt agtagctgac aacttccaat tgctggaaag
    21301 tcgttctgca tctgaaaatc gccaacaaag cggcggattc caaagttctg gtcaatcagc
    21361 tggcggattt ggcggaaaca ataactctaa tcaaacatct caatcatcca acggtatgcc
    21421 tgatttcgac cgtgatactt ctgacccatt cggctcaagt tcaacgatcg acatttcaga
    21481 cgatgattta ccattctaaa cgaatttgat aggagggaaa tagaatggca caacaaagaa
    21541 gaggcggacg taaacgtcgt aaagtagact acattgctgc taaccatatt gaatatatcg
    21601 attataaaga tatcgaatta ctaaaaagat ttatttcaga acgcggtaaa atcttgccac
    21661 gtcgtgtaac tggtacaggc gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg
    21721 ctcgtatcat gggcttgcta cctttcgtta gcgaagacta ataaatccta ggaattgaat
    21781 gactgatcct tttgatcagc tattcaattc ctttttttct gtaaaaataa cctctattcg
    21841 tttagtcgct cgaatgacga aagttatggt aaaatcaaat tagtcatata tgggaggaaa
    21901 attatggaaa ataaaaacaa tcatttatcc ccgaaacctc tcttccttat cctgtttata
    21961 ttaatcgaag tattggtagt cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt
    22021 ctattgatga atttatgggg tgttcgctat tggttgaaaa tgaaacgttg gcttatttta
    22081 aataaacgtg aaaaaatcca gcaggcttcg actgtagctg aaaccaatct agcttatgtc
    22141 tcagaagtca tgcctgtagg agtgattgct tatcagccat caagccaaaa aatcgaatgg
    22201 ctcaatccat tagcattgaa aatcaaagat caaagtggat taacagagga agaattgcta
    22261 gatacttttt ttactgcaaa agaaaaaaag aaaaaccaag tctatctagc agataaaaca
    22321 tttcagtttg atatggatac agtaaagggt gttgtttatt ttaatgatat tacccaaaac
    22381 cagttgttaa aactccatca gacgaacagt cagccggtga ttggtatttt atctattgat
    22441 aactatgcag atgccattga taaattagat gacaaagaga tttcttattt gaactctttc
    22501 gtcactacac tagtttctga ctggatgaat gaataccatg tctttttcaa aagaatcaac
    22561 gcagaacggt ttttcttcgt agcacagatg atcgatctga aaaagatgat ggataaaaaa
    22621 tttgatttgc tggatcgctt tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt
    22681 agtatgggga ttgcttatgg ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat
    22741 aatttagatg ttgctttagt ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa
    22801 ggagcaaaac ctgtttatta tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt
    22861 cgttctcgag cgatgagtac cgctttgcgt cggatttttg atgaaacagg cgatgtgttt
    22921 atcatggggc acaagtttcc ggatatggat gctttaggct ctgcttttgg tgtcagttgt
    22981 ctggcacgct tcaaccagaa aaaagcttac atcgttatca atgagaacga gatcattcca
    23041 gatgtagaac ggtgtcttga agaaatccat aagcatcctg aattagaaat gcaactaatc
    23101 tctccagaac gtgccataga gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac
    23161 cataaacctt ctctatcgat ctctcagggc ctatatgagc aatttgataa gattgtgatc
    23221 attgatcacc atcgtcgtgg ggaagagttt ccaagtaaac ctctgctgac ttatattgaa
    23281 tcttcagctt cttcagcttc agagctagtt tctgagctga tccaatacca aagcagcaaa
    23341 acaaatcgct tagatcgttt tgaggccaca ttattgcttg cagggatggt tgtagataca
    23401 aatagtttca atgtccgtac aactgcacga acttttgacg ttgccagcta tttaaggtct
    23461 tgtggagcag acgcatcctt agtacaatat ttattgagct cggatttagc gacatacttg
    23521 gagatcagca atttgattgc tggaagcgac tatgtgactt ctgatattgt tgtggcggca
    23581 gggacagacg aaaaagagta taatagtgtt atagcagcaa aaacagcaga tacattgctt
    23641 tcgatgatca atatcaatgc agctttcgtc attacaaagc ggacagacgg cttgattggg
    23701 atcagtgcca gaagtaccgg cacgatcaac gtccaaatca tcatggaatc cttaggtggc
    23761 ggaggacact tcacaaacgc agctactcaa ctggaaaata cgacagttga ggaaacaaaa
    23821 cagaagctat ttgctgcaat caaagaaaat atgaatcaaa tatatgataa ggagtaatcc
    23881 aaatgaaagt aattttttta caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag
    23941 aagttccagc aggttatgca caaaattatc taatcaaaaa taacttggca aaagaagcaa
    24001 cgaaaagcag tatcagtcag ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg
    24061 aaatcttgga agaagctaaa aagttaaaag aattcttgga aaaagaagat actgtagtcg
    24121 aattgaaggc gaaagcaggg gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa
    24181 ttgctgaagg gttaaacaaa caatacaaaa tcaaattaga taaacgaaaa atcgaattag
    24241 aaaatccgat ccgttcacta ggctacacaa acgtacctgt taagcttcat catgaggtga
    24301 cggctacgat caaagttcat gtcgtacaag aataacaaat cttgatagcg atgttcagct
    24361 ggtaagtata accagaagga aaaaagacaa accgaaattt atctgagaaa acagctgcag
    24421 caatgaaagc aaaagctgca aatcgatttc agattgattt cggtttcttt tttgggaaca
    24481 gcaaaatagc ataaagaaaa cgagtataat aaaagagaac tattttttta ttacactttc
    24541 ggtacgaatg atgacttttc tctcattttc ttgtaaaatg aagcaattga agacaaggag
    24601 cgatacgtat gaatgaagtg tggcaggatc gaatcccacc gcaaaatatt gaagctgaac
    24661 aagcggtttt aggctctgtt tttcttgacg cagaaacgat cattgatgcc atggagtaca
    24721 tagagccaaa agacttttat cgtcgtggcc atcaaattat ttttgagaca atgatcgagt
    24781 taaatgatcg taatgaagcg attgacgtgg ttactgtcaa agaccggctg gaacaagcca
    24841 atttgttaga agatgccggt ggtttaagtt atctctcaga cttggcatta gcagttccga
    24901 cagcagcaaa tatcgtctac tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat
    24961 tgatccagac agcaacagga atcgttacga aaggattcga gcaaggagaa gaagttcaaa
    25021 caattttaga tgatgccgaa cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg
    25081 gctttttatc tattgctgaa gtactgaact ccacgattga aaatatcgat cgactagctc
    25141 aaaacaatga agaaatcaca ggtctaccaa ctggctatca aggtctggat aaaatgacag
    25201 caggtttcca gcccgaagaa ctaatcattc ttgctgcacg tccagcggta ggtaaaacag
    25261 cgtttgcatt gaatattgct caaaatgtag gaacaaaaac agatcgttct gttgcgatat
    25321 ttagtttgga aatgggggca gaatctcttg taaatcggat gctttgtgca gaaggttcga
    25381 ttgaggccag ccatttacgt acaggtcagc tttcggaaga agaatggtcg aatctgatcg
    25441 ttgcgatggg gagtctttct cgagcaaata tttttatcga tgatacacca gggatcaaaa
    25501 tctctgaaat ccgtgcgaaa tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga
    25561 tattgatcga ttatttgcag ttgattgaag gtaacggtaa agagaaccgt caacaagaag
    25621 tatcagatat ctctcgccaa ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg
    25681 ctctttcaca gttatctcgt ggagtagaac agcgacaaga caaacgacca gtgctaagtg
    25741 atattcgtga atccggatca attgaacaag atgcggacat cgtagccttc ttgtatcgag
    25801 atgattatta tgaaagagaa ggcggagatg acgatgatgg aaacgagcca aaaaatgaca
    25861 acgtcatcga agtcatcatc gagaagaacc gaagcggtgc acgaggaaca gttgaactac
    25921 tgtttatcaa agaatacaat aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa
    25981 gaggttgtga gccttgagca atcccgagag ctctcgtaag aagatgcaag gagtaaccgc
    26041 aggaacgact agagtactta atttaaacag atctccaaag ggattgtgac aaaagtcttg
    26101 tcacagtccc tttttccgaa taaacggtgt tcaatcagct gacccttggt attagctcag
    26161 caaacg
//
LOCUS       DACECR010000034        25871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.034, whole genome shotgun sequence.
ACCESSION   DACECR010000034 DACECR010000000
VERSION     DACECR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.034"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(441..1751)
                     /locus_tag="IWO17_001293"
     CDS             complement(441..1751)
                     /locus_tag="IWO17_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0290179.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1763..3181)
                     /locus_tag="IWO17_001294"
     CDS             complement(1763..3181)
                     /locus_tag="IWO17_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0290180.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3421..4989)
                     /locus_tag="IWO17_001295"
     CDS             complement(3421..4989)
                     /locus_tag="IWO17_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ0290181.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4993..5403)
                     /locus_tag="IWO17_001296"
     CDS             complement(4993..5403)
                     /locus_tag="IWO17_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0290182.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5400..6323)
                     /locus_tag="IWO17_001297"
     CDS             complement(5400..6323)
                     /locus_tag="IWO17_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ0290183.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6320..7198)
                     /locus_tag="IWO17_001298"
     CDS             complement(6320..7198)
                     /locus_tag="IWO17_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0290184.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7185..8522)
                     /locus_tag="IWO17_001299"
     CDS             complement(7185..8522)
                     /locus_tag="IWO17_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0290185.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8525..9628)
                     /locus_tag="IWO17_001300"
     CDS             complement(8525..9628)
                     /locus_tag="IWO17_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0290186.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9702..11210)
                     /locus_tag="IWO17_001301"
     CDS             complement(9702..11210)
                     /locus_tag="IWO17_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0290187.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11176..11868)
                     /locus_tag="IWO17_001302"
     CDS             complement(11176..11868)
                     /locus_tag="IWO17_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0290188.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11890..12864)
                     /locus_tag="IWO17_001303"
     CDS             complement(11890..12864)
                     /locus_tag="IWO17_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0290189.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13164..14036
                     /locus_tag="IWO17_001304"
     CDS             13164..14036
                     /locus_tag="IWO17_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0290190.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14494..16692)
                     /locus_tag="IWO17_001305"
     CDS             complement(14494..16692)
                     /locus_tag="IWO17_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0290191.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16924..17916
                     /locus_tag="IWO17_001306"
     CDS             16924..17916
                     /locus_tag="IWO17_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0290192.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17976..19397
                     /locus_tag="IWO17_001307"
     CDS             17976..19397
                     /locus_tag="IWO17_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ0290193.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19390..20889
                     /locus_tag="IWO17_001308"
     CDS             19390..20889
                     /locus_tag="IWO17_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ0290194.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20928..22322
                     /gene="uxaC"
                     /locus_tag="IWO17_001309"
     CDS             20928..22322
                     /gene="uxaC"
                     /locus_tag="IWO17_001309"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0290195.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22481..23164
                     /locus_tag="IWO17_001310"
     CDS             22481..23164
                     /locus_tag="IWO17_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ0290196.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23166..24002
                     /locus_tag="IWO17_001311"
     CDS             23166..24002
                     /locus_tag="IWO17_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0290197.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24013..25767
                     /locus_tag="IWO17_001312"
     CDS             24013..25767
                     /locus_tag="IWO17_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0290198.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         7874 a         5263 c         4532 g         8202 t
ORIGIN      
        1 ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt caaaggtcta
       61 cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt atatcatatc
      121 agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg tttttcgaaa
      181 tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa cttttttgaa
      241 gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta gttatcttat
      301 cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt tcaatgaagc
      361 gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa atattattta
      421 agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca
      481 gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc gattcgcccg
      541 aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg cttttcagga
      601 aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag gcatgcctgt
      661 acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg tatttcttca
      721 gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt tctattgtat
      781 tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat attcagattt
      841 tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat tcgaatacca
      901 cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc agcactaact
      961 tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac ctcgattctt
     1021 gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat agcaatacaa
     1081 tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga atcaatatca
     1141 atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac taatacatcg
     1201 ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca
     1261 acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc ataatccgca
     1321 atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg atcttttccc
     1381 caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc ttctataaga
     1441 atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc tacgccggct
     1501 actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag gactgcacct
     1561 tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa ataaatgcct
     1621 tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt ttgcagtatt
     1681 attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat aatccctttt
     1741 tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct tcaccaataa
     1801 atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg gatacaaata
     1861 agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact aaaactgatt
     1921 ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata aatccgccta
     1981 ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca tatactgttc
     2041 catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta atgaatgccc
     2101 agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga atcagatata
     2161 cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa gaagcaagta
     2221 acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta gcaaatggtg
     2281 ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct tgtttattat
     2341 ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga ttgatcacaa
     2401 tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa ccttttacca
     2461 actgagaagc agatactttt tcgtttttga caactcttac tctttcagta gtcaatttac
     2521 aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc caaaagaaat
     2581 gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa atcggaatga
     2641 agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg
     2701 tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg atattgattg
     2761 ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca
     2821 ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa aatggatact
     2881 tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag acatcacata
     2941 atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag aataacgtac
     3001 ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag aaactattga
     3061 ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc ttatcggaaa
     3121 tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt tgtgaaagca
     3181 tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct ttttttcttg
     3241 tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa aaacaaaaaa
     3301 taatctaaaa acattgatgc ataagattga taactttttt tacataacaa tatatacaaa
     3361 caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta aagaattaaa
     3421 ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac ttcctctttt
     3481 tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc ctgagagtat
     3541 acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt ctttcaaatg
     3601 aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg
     3661 aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac gtaggcattt
     3721 tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat ttaagtacag
     3781 taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg tctccgcatc
     3841 aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag attgaagaga
     3901 ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc aatcagcatt
     3961 tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg tatccataaa
     4021 ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt cttccatcgg
     4081 agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca cttcttgaat
     4141 agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa gatatgtcat
     4201 gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt gcaatgtaga
     4261 agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt aaggaccagt
     4321 aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc cttctgccgt
     4381 catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt tcgctaattc
     4441 tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg catcatattc
     4501 ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc atagttcatt
     4561 gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa agccgttact
     4621 atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga tcaaataatt
     4681 gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa gataaaacaa
     4741 tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat ttccttgcaa
     4801 agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat ttttcgactc
     4861 atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat accgctcttt
     4921 cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt tcactttgat
     4981 agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga atgccccagc
     5041 tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca acaatatgaa
     5101 tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag cagtcaaaaa
     5161 caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca gaccagtcat
     5221 gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta cagcaagcga
     5281 ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg ctaacccgcc
     5341 aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc attttctttt
     5401 catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat cgatgtttca
     5461 taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc atcccaatta
     5521 tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa gacatttgca
     5581 tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc agtcaaggta
     5641 cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc tgtgatgtat
     5701 ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc atctgcccca
     5761 ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg acgacaatac
     5821 tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa atcaggcaac
     5881 ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa agatacgcga
     5941 tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc tgctgtattt
     6001 tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt agcacttcgg
     6061 aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt accggagata
     6121 actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat atattcatca
     6181 tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc tgccaaataa
     6241 tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt ctttccaatt
     6301 atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa atatttctca
     6361 tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt tctggactac
     6421 ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct tcgtccaagc
     6481 acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt ttttcttgga
     6541 ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac ccatgatgat
     6601 ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga tcaatacaaa
     6661 ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc atagaaagac
     6721 caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa tcgatcaagc
     6781 tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa aattgtttga
     6841 acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca atcgaccaaa
     6901 cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag taaggagcac
     6961 catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc aggtaaagga
     7021 ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact gtttccaatt
     7081 catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct cggtataatc
     7141 gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc aactgcataa
     7201 gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga ttggaatttc
     7261 tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg attttcaatc
     7321 gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg tctgctcatt
     7381 tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc catcgagaca
     7441 tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata aataaaacct
     7501 gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct gaatgctggt
     7561 gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt ccctcgcgga
     7621 ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat gactatattg
     7681 gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa tcgtattcct
     7741 cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact accactcatt
     7801 tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt gatcgtgata
     7861 ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc aatacagtca
     7921 tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc tggatcaatt
     7981 ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa cgttgcatta
     8041 ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat atctttcaca
     8101 gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc gagattatct
     8161 ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt accgaaacca
     8221 gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac ttttcgatgg
     8281 actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga gaatttcaga
     8341 accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc tcctgttaga
     8401 aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc atcaatggct
     8461 tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa aatatcatac
     8521 atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg cctttttgat
     8581 cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc tgatctgctc
     8641 cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct tttgtttcca
     8701 gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc cattcttctt
     8761 tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata ctactgccgg
     8821 aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac catgtcccgc
     8881 tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt tctttcagca
     8941 aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc atcaaatacc
     9001 agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca gcccatggtt
     9061 ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg aatagatgtt
     9121 tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca ctaaaattct
     9181 ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa atttgataag
     9241 gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt gccaactgct
     9301 gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg tacaataaaa
     9361 agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg tagccacgga
     9421 tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca aaataaattt
     9481 tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca tagtcataag
     9541 accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc atgatacttt
     9601 ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt aaaataggat
     9661 ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc cgctgctttg
     9721 tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca gtcttctaca
     9781 gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt agcatcaatg
     9841 acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa tggcacagaa
     9901 ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa ttctttttca
     9961 gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga taacggttgc
    10021 acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc attcaattca
    10081 taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc taaaagatca
    10141 tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga tttgggaaat
    10201 gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc gccttcacca
    10261 tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg acccatcgtc
    10321 ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg tgcaaattgt
    10381 tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc attctcaaat
    10441 agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc tggagtgaat
    10501 ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt gaaaagttta
    10561 aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt gtcgtccaca
    10621 ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag ctcatccatg
    10681 gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac taagccagca
    10741 cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact cgcagcttcg
    10801 attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc tgatacatcc
    10861 cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat cgcaatttga
    10921 agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct ttcttctttt
    10981 gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc gatcaagttc
    11041 acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt gattttagct
    11101 tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc ggcaagtgct
    11161 agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat aaattttctc
    11221 cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg agttcttcca
    11281 ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga agagactgcc
    11341 accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa tccctaccat
    11401 acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa atgccatttt
    11461 cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa tatagagtgc
    11521 tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc aattgtgtat
    11581 gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc tcatcagcaa
    11641 ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag gaaacgtttc
    11701 ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag gaccgaaagt
    11761 aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat aaaccaactg
    11821 taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat ctttccctct
    11881 ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat tagctaaaat
    11941 taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga aacttaactg
    12001 tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa cgcttcgatt
    12061 caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct gaccgatttt
    12121 tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat gccatgtttt
    12181 gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat cgactgcagt
    12241 tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca cttgtccgat
    12301 atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa atcccattgt
    12361 tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag taatcgaaac
    12421 aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc cgtttttcaa
    12481 tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg gaaaaaacac
    12541 gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga atgtcggttc
    12601 tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta ttcctaaaaa
    12661 tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc taaaaatcaa
    12721 gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt tgattcctgc
    12781 aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca gttgaaatgg
    12841 ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt ttgctgctta
    12901 tgacttcatg atagataact atctgaatta cgtatataat catttttacc gtaagtaaaa
    12961 acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt ttttcacaaa
    13021 taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat aggaaatgat
    13081 tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa catttaattt
    13141 attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa agaagtttta
    13201 cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt ccttttttaa
    13261 atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag tttatccttt
    13321 ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg accattttta
    13381 gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt atgtgtgcaa
    13441 cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt ttcgtcaact
    13501 ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc ttagttgttc
    13561 gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct gttaatgcaa
    13621 tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg gaagaatcag
    13681 caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg ccattagcgt
    13741 taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac acttatatga
    13801 ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt ttacgccaaa
    13861 tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg attccaccag
    13921 cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg attgtttatc
    13981 cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa gggtgattat
    14041 gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa taagaaggca
    14101 tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg aagaatctac
    14161 tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag cgatgataaa
    14221 aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga ttttgaaaaa
    14281 tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc ttgaacgcca
    14341 tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg cgtgcttagc
    14401 cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc ttttattgct
    14461 tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc gatattctcc
    14521 cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat ttcgatattg
    14581 caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt tttttgctac
    14641 ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct ttttaaatac
    14701 attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta aagacagatc
    14761 acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt taaattcatg
    14821 atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta aaacaagttt
    14881 ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt tcatgttatc
    14941 aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat tgatcaagcg
    15001 gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg catcaatcaa
    15061 ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa acaatcgatt
    15121 ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa cgatttcctg
    15181 ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt cttttgcccg
    15241 atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc ctatactgat
    15301 agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact ccatgacttt
    15361 tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca tttcttcatt
    15421 caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca tgttgttgat
    15481 cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat cgatttgaac
    15541 caaagctgta taatagcagt cattttgaga actataacca aattgctgca accgctgatc
    15601 tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg tttccagctg
    15661 tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga tccgactgac
    15721 tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa gattcagacg
    15781 ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg aaagaagacc
    15841 aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat tgcggatcat
    15901 tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat aatctgattt
    15961 cagtactata tacgttttat ctttttctct tatcactttc agctgattgt aatctagatc
    16021 cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag taaaaaggtt
    16081 gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa cttgttctat
    16141 tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa acattggcaa
    16201 agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat ttttgttatt
    16261 tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca acgatccatt
    16321 gattactccc catttaccag ccaaactgac taaatcataa ttattatcat ccataacaat
    16381 cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat ctgtatatgt
    16441 cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag aaaacgaata
    16501 ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca aattcttttt
    16561 gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga ataaactaaa
    16621 tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat aaaatactgt
    16681 acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat ttttctgtaa
    16741 aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca taattaaaaa
    16801 taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc tattgactta
    16861 tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa ggaaaatgat
    16921 ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac gacagtttct
    16981 cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat acgacaacta
    17041 gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa tcaaaaaaaa
    17101 tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa tttcctagca
    17161 gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc tgttgaaagt
    17221 atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact gatcgttatg
    17281 agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga atttcctttc
    17341 gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc cgatgatgcg
    17401 gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa gatcgcttat
    17461 attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt attgaaaagt
    17521 ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga ttacagctta
    17581 gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc tactctggtt
    17641 ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt agctggcctc
    17701 cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt ctcctatctc
    17761 aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga aggaacaaag
    17821 cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga gctctccacc
    17881 ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt tatataaaaa
    17941 tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca aaagaattag
    18001 tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat tttatgcgtg
    18061 gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa ggtaatgttg
    18121 caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa gataatttat
    18181 atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc gagattatca
    18241 ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta tctctagcag
    18301 aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc caattcgtgg
    18361 agactgataa tttaacagat actcccccac agagcctccc gggcaaactg acagcctttt
    18421 tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc gaactgattg
    18481 acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa tgggatttag
    18541 aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt ttagttgatc
    18601 gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg cataactatg
    18661 aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa gggcctaaag
    18721 aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc acagatgata
    18781 tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca gcgatggtcc
    18841 ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat cctttattct
    18901 ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct ttacctaaag
    18961 atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct tttgcccatc
    19021 acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt ctgcttccag
    19081 tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact gtttcactag
    19141 ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat gaaaagacga
    19201 taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga ttattgcaaa
    19261 acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat acagttcaag
    19321 aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa atgaaaggaa
    19381 cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg agacagtgtg
    19441 gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca gcaaacgatc
    19501 accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt gaaagctgga
    19561 gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt aactgccggt
    19621 caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga ttatcgatat
    19681 gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca aggttacttg
    19741 cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac cgttgggtgt
    19801 gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc agatctggga
    19861 cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct tggaaaagat
    19921 catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc cggaggagtt
    19981 ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa aattttaggt
    20041 gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga tgagatcgaa
    20101 caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca gcgcatatca
    20161 gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg tctatcggga
    20221 ataactgcta atcctttact tggtgcattt tctgactact tgattgctca aggaggcagc
    20281 acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat ggcacgagcg
    20341 gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa acagtatttc
    20401 ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc aggtgggatc
    20461 actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc tcctgtcgtt
    20521 gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt gcaagctccc
    20581 gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt ggtcttgttt
    20641 accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt atctacgaat
    20701 acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt cttattagaa
    20761 caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt agctagtgga
    20821 gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa aaacggagta
    20881 acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg tttttatctg
    20941 atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac gcaaaaaaca
    21001 tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa aacaacaatt
    21061 tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc ttgatgcgag
    21121 cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa aaatttttag
    21181 cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg tcacatcttg
    21241 aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca gagaaaattt
    21301 ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa atcgcaaaaa
    21361 aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg cactatcatc
    21421 agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt ccagataaag
    21481 cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa gaagcagctg
    21541 accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg atagaatact
    21601 tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc tataaagaag
    21661 cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa ctattatctt
    21721 cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac ttctatcatt
    21781 ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat acacaaatgt
    21841 tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct ttgactattc
    21901 ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag acgatccttt
    21961 attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt ttccaaaaag
    22021 aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact cgagctggta
    22081 tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt gtcggcatgc
    22141 tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt cgtgttcttt
    22201 gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa caacttttag
    22261 gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc ttttttgcct
    22321 aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac ggaatccgtt
    22381 tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct cttgctgttc
    22441 tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta tccatattgc
    22501 cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag gttggggaga
    22561 aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa agaatggacg
    22621 cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac agtttcagcc
    22681 gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag aagaaagagg
    22741 tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat gcgcaaaaaa
    22801 agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact tagaaaacgg
    22861 aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat ttgctgagaa
    22921 acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc agaccttctc
    22981 aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta aaaattaccc
    23041 agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag ttgcccgaat
    23101 aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac aaaaaggagt
    23161 ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt cgaaatcgtg
    23221 aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt gcaccacaaa
    23281 aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct gcctattttg
    23341 gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat atcaacctat
    23401 ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag caaattgctc
    23461 tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc tctgtcggtt
    23521 acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct atcattgaat
    23581 tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg atcaatcatc
    23641 caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca ccaaatttaa
    23701 aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat caggaaaaag
    23761 tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat ctcaaagact
    23821 atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt ttagatttcc
    23881 gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact cttgaagcta
    23941 ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac caagattgtt
    24001 aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca tgcaaatgct
    24061 tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat gtcaggactc
    24121 ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc caatgtaatc
    24181 aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa cgcttttttg
    24241 atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa ataccatagc
    24301 acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa aatccaagaa
    24361 ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa gatcatccgt
    24421 gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt aaatatcgct
    24481 gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt tttcatgttt
    24541 ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa aaggatcaga
    24601 cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt gatcgaagtg
    24661 atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac aactcgctta
    24721 aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct gcaatctgtt
    24781 ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt gattttcact
    24841 gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta tcagacgtat
    24901 ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac gatttcaaaa
    24961 ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt agaaacttat
    25021 cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga tgtttctttt
    25081 ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt gaaagctgga
    25141 gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct taacttattg
    25201 attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc tttggatgaa
    25261 ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac tttgttgttc
    25321 tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa agaacgttta
    25381 gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc tgatggtctg
    25441 gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca acgtatctcc
    25501 attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc aacctcagct
    25561 ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac agaaacaccc
    25621 actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa aatcgttgtg
    25681 atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc tcaaaaaggc
    25741 tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag acttttgtcg
    25801 tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat
    25861 agtcatcaaa c
//
LOCUS       DACECR010000035        25676 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.035, whole genome shotgun sequence.
ACCESSION   DACECR010000035 DACECR010000000
VERSION     DACECR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.035"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            171..515
                     /locus_tag="IWO17_001313"
     CDS             171..515
                     /locus_tag="IWO17_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ0290199.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="IWO17_001314"
                     /pseudo
     CDS             516..935
                     /locus_tag="IWO17_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1027..1377)
                     /locus_tag="IWO17_001315"
     CDS             complement(1027..1377)
                     /locus_tag="IWO17_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290200.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="IWO17_001316"
     CDS             complement(1343..2674)
                     /locus_tag="IWO17_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ0290201.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="IWO17_001317"
     CDS             2930..3496
                     /locus_tag="IWO17_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0290202.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="IWO17_001318"
     tRNA            3641..3728
                     /locus_tag="IWO17_001318"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            4062..5036
                     /locus_tag="IWO17_001319"
     CDS             4062..5036
                     /locus_tag="IWO17_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0290203.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5361..7256
                     /gene="asnB"
                     /locus_tag="IWO17_001320"
     CDS             5361..7256
                     /gene="asnB"
                     /locus_tag="IWO17_001320"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ0290204.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7451..7897)
                     /locus_tag="IWO17_001321"
     CDS             complement(7451..7897)
                     /locus_tag="IWO17_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0290205.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8125..8274
                     /gene="rpmG"
                     /locus_tag="IWO17_001322"
     CDS             8125..8274
                     /gene="rpmG"
                     /locus_tag="IWO17_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0290206.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8362..8880
                     /locus_tag="IWO17_001323"
     CDS             8362..8880
                     /locus_tag="IWO17_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0290207.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8957..9913)
                     /locus_tag="IWO17_001324"
     CDS             complement(8957..9913)
                     /locus_tag="IWO17_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0290208.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10079..10885
                     /locus_tag="IWO17_001325"
     CDS             10079..10885
                     /locus_tag="IWO17_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0290209.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10882..11871
                     /locus_tag="IWO17_001326"
     CDS             10882..11871
                     /locus_tag="IWO17_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0290210.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11868..12872
                     /locus_tag="IWO17_001327"
     CDS             11868..12872
                     /locus_tag="IWO17_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0290211.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13004..13546
                     /locus_tag="IWO17_001328"
     CDS             13004..13546
                     /locus_tag="IWO17_001328"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ0290212.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13566..14264
                     /locus_tag="IWO17_001329"
     CDS             13566..14264
                     /locus_tag="IWO17_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ0290213.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14285..14497
                     /locus_tag="IWO17_001330"
     CDS             14285..14497
                     /locus_tag="IWO17_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ0290214.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14497..15459
                     /locus_tag="IWO17_001331"
     CDS             14497..15459
                     /locus_tag="IWO17_001331"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ0290215.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15477..15872
                     /locus_tag="IWO17_001332"
     CDS             15477..15872
                     /locus_tag="IWO17_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0290216.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16035..16400
                     /locus_tag="IWO17_001333"
     CDS             16035..16400
                     /locus_tag="IWO17_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ0290217.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16397..18208
                     /locus_tag="IWO17_001334"
     CDS             16397..18208
                     /locus_tag="IWO17_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ0290218.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18270..18437)
                     /locus_tag="IWO17_001335"
     CDS             complement(18270..18437)
                     /locus_tag="IWO17_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ0290219.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18685..19674
                     /gene="galE"
                     /locus_tag="IWO17_001336"
     CDS             18685..19674
                     /gene="galE"
                     /locus_tag="IWO17_001336"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ0290220.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19686..21170
                     /gene="galT"
                     /locus_tag="IWO17_001337"
     CDS             19686..21170
                     /gene="galT"
                     /locus_tag="IWO17_001337"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ0290221.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21194..22174
                     /locus_tag="IWO17_001338"
     CDS             21194..22174
                     /locus_tag="IWO17_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0290222.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22263..23087
                     /gene="tsaB"
                     /locus_tag="IWO17_001339"
     CDS             22263..23087
                     /gene="tsaB"
                     /locus_tag="IWO17_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ0290223.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23071..23613
                     /gene="rimI"
                     /locus_tag="IWO17_001340"
     CDS             23071..23613
                     /gene="rimI"
                     /locus_tag="IWO17_001340"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0290224.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23620..24084
                     /gene="rimI"
                     /locus_tag="IWO17_001341"
     CDS             23620..24084
                     /gene="rimI"
                     /locus_tag="IWO17_001341"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0290225.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24081..25097
                     /gene="tsaD"
                     /locus_tag="IWO17_001342"
     CDS             24081..25097
                     /gene="tsaD"
                     /locus_tag="IWO17_001342"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ0290226.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8329 a         4398 c         5378 g         7571 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggtaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc
     3901 cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt
     3961 ataaatgcta gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta
     4021 agataaaagt gtaaaaacga tttgttttag gacgggaaac gatgtgtacc gcaatcactt
     4081 atgcaacgaa agaccattat tttggaagga attttgatta tgagatgtct tataatgaag
     4141 tggtcactat tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata
     4201 aacattatgc aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg
     4261 ctacaaatga aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata
     4321 aagaattaca agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag
     4381 ggcaatgctc aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata
     4441 tcgattttag tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa
     4501 aatcgatagt aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg
     4561 tgttgacaaa taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc
     4621 tatcaaatgg aacaccagaa aatcattttt caaaccagat cagcttgaat gtttatagtc
     4681 gtgggatggg aggattgggc ttacctggag atctctcttc tgtttctcga tttgtaaaag
     4741 ccacatttac taaaatgaat gctgcatcag gtgattcaga atctgaaagt atcagtcaat
     4801 tttttcatat actaggctca gtcgagcaac aaaaaggagt atgcgatact ggagaaagaa
     4861 aatacgaata tacgatttat tcttcttgct gtaacgttga taaagggatt tattattatc
     4921 gtacgtatga agacagtcaa attactgctg taaatatgaa taaagaagac ttggatcgtc
     4981 atgaattggt cagttatcca gtcataaaag agcaacagat aaactatgtc aattaattaa
     5041 tgaatagatc cgtttggtat catattttct gatacagtca taattagaca cgatctttag
     5101 gtcgtgtctt tttttgctgg tttttttgga aatagcatcc actaatattt ttttaacacg
     5161 aagagaataa ggtagcctca aaaaagaaaa catggtaaat tagatattgg gactttattt
     5221 tctgtaaaaa cagaaacttt catcttttgt tttcacgtga cgaaattgta agagagaaaa
     5281 tattcgtccg ctatttattc tatgataaga tgcatatgaa tgaaataaac agtaatttta
     5341 tattaggatg tgaaagttaa atgtgcggta ttgtaggttt tgttaacgac aaggacaaca
     5401 aaaaaacaat tattaataca atgatggatc gaatcgttca ccgtggacct aatagttcag
     5461 gagaatatat cgataaacat gttgctttag gattcagaag attaagtatt attgacttag
     5521 agggcggtac tcagcctatt tacaatgaag atcggacaaa gatcattatt tttaatggcg
     5581 aaatctataa ttaccagcct ttgagagaag aattgattgc agctggccac gtttttcaaa
     5641 cacatgcaga tacagaagtc cttttgcatg gttacgaaga atggggaaca gaattattgc
     5701 aaaagatccg cggaatgttt gcttttgcta tttgggataa tgaaaagaat gagttattcg
     5761 gtgcaagaga ccactttggg atcaaaccat attattatgc agaaatgaac ggaacattta
     5821 tgtttggttc agaaatcaaa agttttctgc ctcatcctga ttttaacaaa gaattgaaca
     5881 aagaagcgtt gaaaccttat atgactttcc aatattctcc attaaacggc gaaacattct
     5941 tcaaagatgt ctatcgtttg cctgaaggcc attattatac gtataaagat ggaaaaatag
     6001 acattcagca atattgggat gcagattttg aaacaaaaga aacgcattcc cgacaagaat
     6061 ggatcgaaaa aatcgacgaa accgttcaag cttctattga agcacatacg gtcagtgatg
     6121 tagaagttgg ttctttctta tccagcgggg ttgattccag ttatgtaacc tctgtattga
     6181 aaccagatca ttctttctca atcgggtttg atgataaaac ctataatgaa gcgattgaag
     6241 ccagaaaact aaccgaacta ttggacttag ataatacagc agcagtgatc gacggagaca
     6301 tgtcgttcaa agcattccct ttgatccagt atcacttaga tgaacctgat tcaaatcctt
     6361 cttgcgtacc tttgtacttt ttagcaaatc ttgcttcaca aagtgtgcgg gtagtccaat
     6421 ctggagaagg ggcggatgaa ctgtttgcgg gataccaaac gtatggattc catacgaatt
     6481 caaaattcat ccgtgtgatt gctcaaggat tgaaaaaatt accaaaagga acacgctata
     6541 atttaggacg taaaatcgga aaaatgaaaa atttccatgg acggatccat ttatatgaat
     6601 cacttgctcc tgcaaaagaa tattttatcg gtcatgctcg agtgttcgaa gaatcagaag
     6661 catcagaagt gttaacacca aaatatcaaa cagcaccatc tgtggatgaa atcatgactg
     6721 ttcattacga gaaaacagaa ggaatcaaag atgaagtcaa taagatgcaa tatgtcgatc
     6781 ttcatcaatg gatgccaaaa gatatcttgt taaaggcaga taaactttct atggcaagtt
     6841 ctttggaagt ccgagtacct ttattggata tcgaagtcat gaaattggct caacaaatac
     6901 caagtaagtt tttattgaat caaaacaaca caaaagatat tttcagacaa gctgcgaaca
     6961 aacatcttcc ggaaaaatgg tcgaatcgtg tgaaattagg attccctgtg ccaatcaaag
     7021 cttggttgaa agaagaacac ggctacgaac aagtcaaagc attgttcgaa gcagattttg
     7081 caaaagaatt ttttgatcaa gaaaaaatca tgaagttgct agatgaccat cacgaaggac
     7141 gtaaagaaga gcaaagaaaa atctggacaa tcttcagttt cttgacatgg tataaagtct
     7201 actttgtaga tgaaacgatt ccgcaagcag aagcaatcga ctatgtgact gtttgatgaa
     7261 aatcaaatat caataaaaaa gaggttttct cctaccgttt aacactgagt gataagaaac
     7321 aatttcaaaa aacagctctt cccgttttat agatattatg acttatggtt cggagttggc
     7381 agaggaacac cattgtttcg aacataaggg ggttgtgaca cgattttcgt cacaacccct
     7441 ttacatatgt tcaataatcg atttcttttt cttttgtccc atctttttta taaatgaaca
     7501 aagttgattc tttgttttgt gctacttgct ttcctttttc gattgcttct ttttttgtat
     7561 caaaatggtt gcttgccttt tttgcccctt tagatttcac gatccattgt tcttcctcat
     7621 attctacgat cacatctgaa tcaagcagtt ttccagcacg aggattcgta tcgtgttcat
     7681 ccgtcttgct tggatttttt tctttttcga aagttcgttt ttctgattca ctagcatctt
     7741 cataccattt ttttgcttga tcaatggcaa ttgggatcgc acggttatca gggtaccctt
     7801 catctaataa tgcatttgcg atgtcgattg ctttttttct tgtcaattta tctaagtttt
     7861 tcattgatgc aggaaagtcc ttcatgttcc atggcatatt agtatccccc tttattaaaa
     7921 aacataatct atataaacaa gtgtaagggt atttatacaa aaagaaaaag attttgtttg
     7981 agaagtaaaa ccgttgtatt tatcgtcaag tagttttact taacaaaatc gtgaaaaaca
     8041 ctagcaatct agaaaaaatc atggtaagat aatcaagtct gagagtcatc ttagaggatt
     8101 cttgtaaaaa tgggagggaa aaacatgcgc gtaaacatca ctttagagtg tacttcttgt
     8161 aaagaacgta actatctaac aagcaaaaac aaacgtaaca atcctgatcg tttggaaaaa
     8221 caaaaatatt gcccacgcga acgcaaagtt actttgcacc gtgaaacaaa ataatgtttt
     8281 taaagttctc aattccttgc tggtcaaaga attgagactt tttttatctc aaaacatcta
     8341 aaatcataag gagcggataa tatgtttatt tccatgtggg cacaagataa aaatggtctg
     8401 atcggcaaaa atggtttgtt accatggcga ttgccaaatg atatgcgttt ctttcgcgaa
     8461 catacaatgg atcgaatttt agtgatggga agaaagacct atgaaggaat gggggatctt
     8521 tcacttcctt atcgccatat catcgtgttg acaacgcagc caaattttcg cacgaaagaa
     8581 aatgcggaag taatgcattc gatcgatgaa cttttgtcgt atagtcaaac gatctcagaa
     8641 gatatctatg tttcgggtgg tagtcggatt tttcaagaac ttcttcctta cactggtcgg
     8701 atctggcgaa cattgatcga tagcacgttt gaaggcgata cgtatattgg gaatattgat
     8761 ttttcagact tcgcccttat agaggagtat gaggggataa cggatcaaga aaatctatat
     8821 gcccatcgtt ttcaaaaatg ggaaagagta aaaaataggg ttgcttcaaa agaagaataa
     8881 aatctatgcg tctagtttgt taataagaaa aaaagcatat cacgactaat cgtgaagatg
     8941 ctttttttag ttaaaatcaa tttagttttt ctttgatatc attgaccatt tcctgactgg
     9001 caataggtcc gttatatagc caactagatt caggtccgaa ttgcagtaca tgattgtctg
     9061 tcactgcttt aagattcttc cagcttggtt cattgaacat ggcagcatct ttgtcactgt
     9121 tcacaaggat gatgtagtca gcatctaatt cagctagctt ttcagaggaa acagcagacc
     9181 agtcagaagt ggcttttttg gagacctctt ccactagatc aggaacacca aattccagat
     9241 cgtcgtatac gattcttcca cttgaccgat ttttgctgac cataaaggct gagttgttgg
     9301 tcacccacaa cactgcagct gtcttgttct cgattttatc tgctagttct tctcgagttt
     9361 tcttgatgga tttttgataa tcggaaatga tcttttctgc ctctttttct tttgccaaag
     9421 ctttgccgac atcttttagt tgttcttccc atgtgacatc tgttccgttt tttacaacat
     9481 aagttggcgc gattttgcta tattgttcgt atttcccgcc ttcgacagta gcggaagaag
     9541 aaatcaacag caaatcaggc tcaaagctta acactttctc ataaggaagg tcataagaaa
     9601 tcgttgggac atcctttaaa tcatcttgca gataatcttg aatggaacca ctgccaacag
     9661 tccactgggc gataggtttt tcccctagtg caatcagata gtcttctaag taactgccga
     9721 tgatccgttt gggcgcgttt ggaatctcta ctttatgtcc aagtgtatct gtgactgtat
     9781 gagtactatt ggatgaagtc gtcgaattgg cagttgtagc tgtttcttta tctgaagagg
     9841 gggagcaagc gctcagtact ccagccagca gcaatatgct agataatgtg aatacttttg
     9901 tttttaattt catttctttc ctcctaacat aattgagaat ctttctcatt ggttattatt
     9961 cctgatattt cgaacgctgt caaccgcttc atttggctat agatatgaaa gtagcgcttg
    10021 acgaaatgtt gaaacagcgt cataattgag aatgattttc agcgaaagga tgggtgaaat
    10081 gacgagatta agagcagctg acattgcagc cggctacgaa aacaaaaaaa tcattgaaaa
    10141 catgtctgtt acgattcctg aagggaaaat cactagtttg attgggccaa atggaagtgg
    10201 aaaatctact ttattgaaaa gttttacccg tatactacct gtgaaatcag gaaagatcat
    10261 tgtcaataac tctgatatct ctacatacag ttcaaaagaa cttgcaaaaa aaattgcatt
    10321 attagcacaa actagtgaac accctacagg tatgacagta gaagaagtag tatcttacgg
    10381 acgataccct tatcaaaaac tgttcagtgg catggacacg caagatcatg aagcaattga
    10441 gtgggcgttg gatgctacag gtctagctgg atttcgtatg cgggatatcg caacgctgtc
    10501 tggtggacaa acccagcgtg tctggatcgc tatggcgctt gctcaggaaa cagatatctt
    10561 gatcttggat gaaccgacaa catttttaga tccggcacac caattagaga tcctacagtt
    10621 attgactgaa atcaatcaaa tgaaacaaac aacgatcttg atgtcgattc atgacattaa
    10681 tcatgcttca cgtttttcag attatttatt agcaatgaaa aacggtaagt tgatcacaga
    10741 aggtacacct gaagaagtga tcacaaaaga gtgtatgaaa aaaatatttg agattgatgc
    10801 aacgattgtc acgcttccaa gaacaaataa accagttttt ctaacctatg atttgttgcc
    10861 aaaagagcag gaggatcaat tatgaggact atgaagaaaa atagactcct gcttttggtc
    10921 tgttttgttc ttttgattat tttttttgtt gctctttcaa atggcgcagc gactgtggga
    10981 aaagcagatt tttggcaggc gttgtttgat tttgatagat caaaacaaag tcaccaaatc
    11041 atacgaaata ttcgtttgcc tcgtgtggcg ggagcatttc ttgtagggag ttgttttgca
    11101 ttgagcggtg cattgatgca aggagtgaca agaaatcctt tagcagattc aggcttacta
    11161 ggaatcaacg cgggtgcttc gttggcaatg gcccttagtt ttgctttttg gcctaaagct
    11221 tctgattttt ctgtgtttct aatgtcattg gcaggttctt ttgctgtaac tttgctcgta
    11281 tttggttttt tgcagttttc gaaaatgggc atgaatcctt tacagctgat tttagctggc
    11341 gtagctatta gttctttttt tactgctatc agccaagcac ttacccaatt ctttaatctg
    11401 cagcaggatc ttgctttttg gtttgtaggc ggagctgcca atatcacatg gaaacaatta
    11461 gcagtattgt ttcctttata tatacttgct gttttggttt ctttttcatt aggaaaagca
    11521 atatctttga ttcatttgag tgaaacacat gcattagctc ttggaagtca tcctaaacga
    11581 acgcggttga ttgttttctg tctagttgca gtgttagcag ccattgccgt ttcgttagtc
    11641 ggacctgttt catttattgg gctgttggtt ccgccagttg ttcgcggaat ggttggaacg
    11701 gactatcgtt atgttctacc tgtctctttt ttagttggcg gtggattggt tatgttggcg
    11761 gattttacag cgaggatgat caatccccct tttgaaacac cttttggatt agtcattgca
    11821 ttgattggcg ttccgttttt actgatacaa gtaaggaggg aacaacggtg aaaaaaatat
    11881 attggacgtt ctatttcttg ctttttgtac ttgtcatcct gtctataggt agtttaatga
    11941 ttggtacgcc ttttatttac ctggatcagc tttttgccac gttcataggg aaagaatcat
    12001 cttctttggc gctgattgtt agagaatttc gagttcctcg attactgatc agtttattag
    12061 caggagcttg tcttggcgtt tctggttata tcttgcaagg agtaacaaga aatgaattgg
    12121 cagattctag tatattgggc attaatgccg gagcaggatt tttagtgatg ctttatttag
    12181 gtttcttttc gcactattcg ctgccgtttc tattgctagt tgtagccttt ctaggtggtg
    12241 ttttagctgc ttgctgtgtc tatcttgcgg cctatgatcg aaatggcttt ttgggtatga
    12301 atcgaatctt attgtccggt attgccgtta atgccggcct ttctgcatta acattgctat
    12361 gcacgatcca attatctaag gaaaattatg gatttgtgaa tgcatggtta gcgggatcaa
    12421 tctggggtgc gagctggcct tatgtttggg cattgcttcc atgggcggtt atcttaattc
    12481 cgctaggagg actttatgca cagaggattg tattgctatc atttggtcaa gaacgcgcgc
    12541 aaggattagg ccttaacgtg gctaaaagtc aaaaaatcct tttgttgtta gctgttgctt
    12601 tggcaagcgg tagtgtagcg gtgacaggta gtttatcttt tgtaggacta ctagctcctc
    12661 atgctgcgaa gctagtcgta cgtaaagaaa ataactggac ctttgttctg agcggtttgt
    12721 tcggttccat ttttctgctt agtgcagata tggttgctag agtgattcta ctaagtgggg
    12781 aagtacctac aggaattttg attgcccttt ttggtgcgcc atattttctg tatgcacttt
    12841 ttatcagaca aaaacacgcg ctgaacagtt gaaattcatt aaaaaaggaa ggccaatcat
    12901 ttctaggtct tctttttatt ctatttatgc atttatttga aagttcgatt gctatttttc
    12961 tttttttcct ataccatttt atgagagaag gaagggtgag tttttggacc aattgaaagt
    13021 aagattacgc cggaaaggaa tcgaaaagct gaaaaatctg gccaaaagtg gcaagaaaaa
    13081 ggaaaaagaa cagcgaatct ataaacagct attcgccagc gagtactgga agtcagcaaa
    13141 agtgataggg acgactttat cgaatgaatt tgaactggat acacagccgg ttatccaacg
    13201 agcagaaaaa gaacaaaaga aaatagttgt acctaaaact ttgccgaaac gtcagatggc
    13261 gttttatgaa atggatgaac agtccgttct tgtacgttcg agttttggtg ttcttgagcc
    13321 acaatcagag aagctctatt tgccggagat gatcgatctc ctcattgtac caggtattat
    13381 ctacaataga gccggttatc gaatcggttt tggcggtggt tattacgacc ggtatttaaa
    13441 taattttcat aaaaaaacat gtagccttgt ttttcgtgaa caattgaatg aacaatggca
    13501 accggagagc tttgatcaga agatccaaca attgtttatt gactaaaatg aaatgggagg
    13561 ttttcatgaa ttatcaacag caaataaaat tgcgtgcatg gttaagacga ccgttatgga
    13621 cctatgcttt tttagggata cagacagcgg tcttcctcat gatggaatta tttccgcgac
    13681 ttgagatccc ttattatgcc ggaatgtatg gtccttatct tgttcacttc aatgaatggt
    13741 ggcgtttagt gacaccaatc ttcatccatt ttggactgat gcatttcgta atgaactcac
    13801 tgatactgta ttttatgggg cagcaaatcg aggcaatcta tggccattgg cgttttttct
    13861 tgatttatat gttcagcggg ataatgggaa atacagcaag ctttgctttt aacgaagcga
    13921 atgttctgtc tggtggtgca agtacttcga tattcggttt gttcggtgcg ttgtttatct
    13981 tagggtttca ttttaaacat aacccagcga tccagcagct agtcagacat tttcttttat
    14041 ttattgtgtt gacatttgtt tttggtcttc tggatacctc tgtagatgtt tggggacatg
    14101 tcggtgggct aattggcgga ttagtgttag ggaatgtttt gggtctaccg aaacaacaga
    14161 caagctattc gatacaccaa aggattcttt ctacactagt ttttgtattt ctttttgtaa
    14221 tatgtatttt actgggttta aaaaaatatg gtttacttgt ataatatgaa ggaagtgctt
    14281 aaagatggag agcttatatg atgtacagca gctgttcaaa aaatttggca tctttatcta
    14341 tgtgggtgca cgcatctatg acattgaatt aatgactatc gaattgcgta atctgtatga
    14401 tagccgtttg attgatcatg atacatacat gacagcgtgg cgcatattaa aaagagagca
    14461 ccgaattgaa gaaagccgaa agaaaggaaa tttgtaatgg acaagaagat tattgggatt
    14521 gatttaggtg gaacaacagc aaaatttgca attttaacac cagaaggaga gattcagcaa
    14581 aaatggagca tcgataccaa tattttggat gatggtaagc atatcgttcc tgaaatcatt
    14641 gaatcaatca atcatcgttt gaatttatat aatatgaaag ccgaagactt tatcggaatc
    14701 ggcatgggta ccccaggaag cgtggacagc gaagctggaa cggttattgg tgcgtataac
    14761 ttaaactgga cagaagttca atttgtgaaa aaactgatcg aggcaggaac aggaatcaaa
    14821 ttcgctatcg ataatgacgc gaatgttgct gctttaggtg aacgatggaa aggtgctgga
    14881 gaaaacgatc cagacgttgt attcgttact ctaggaacag gtgttggcgg aggtatcgtt
    14941 gctggcggaa atttgattca cggcgtgagc ggagctggag gagaaatcgg acatatcact
    15001 gtggatcctg aagggttcga atgtacttgc ggtaaacgcg gatgtttaga aactgtatct
    15061 agcgctacag gtgtggtacg cctaggtcgt ttcttggcag aagaatacgc aggggattca
    15121 aaactgaaag caatgctaga caacggagaa gaagtaacaa gtaaagatat ctttgaaatg
    15181 gctcaagaag acgatccgtt tgcattgatg gttgttgatc gcgtatgctt ctacttaggt
    15241 ttagcttgcg gaaacttggg gaatacatta aatccgtcaa gtatcgtgtt aggtggagga
    15301 gtatcggcag caggagaatt tttacgtagc cgtgtagaga aatattttaa agaatttact
    15361 ttcccgcaag taagagaatc aacaaaaatc aaattagcag agcttggtaa tgaagctggt
    15421 gtcattggtg ctgcctcatt agccttgaaa tttgcttaaa aaaacggagg cattacatgg
    15481 ttttatgggt aattaatatc attttgctat tgattgtgct tgcaatcatc ctttgggaag
    15541 tctatctacg agtaatggcc aaacgttcag caacgacttt gacagaagaa gaatttcgag
    15601 aaggaatgag aaaagcacaa gtaatcgatg ttcgcgaaaa agatgtgttt gatgctgggc
    15661 atattttagg tgcaagaaac atcccttaca ctgttttgaa agattcatta ggatctattc
    15721 gaaaagacca gcctgtctat atttacgatc aaaagaaaag tttgagcatc cgtacagcaa
    15781 ataaattacg taaagcaggt tatcaagaga tttatttact taagggtgga tatgacggat
    15841 ggtctggaaa aatcaaaagt aaaaaagtat aaaacagatg attgtatgac agctttaaaa
    15901 accgaatcaa tcattagatt ttgaccaaga tcacatgaac ggcgcaatgg atagttgtgc
    15961 cgttcatttt ttttattttg tgctattatc tacatatata agctaagcga aaaggtagaa
    16021 agaaggaaac aactatgatc aaattaaaac gtgtttacct tgatgctgaa aaagaggatg
    16081 gctatagaat actcgtggat agattctggc cacgaggaat ttcaaaagaa aaagcgcagt
    16141 tagatctgtg gttgaaagaa attgcaccta gtaatgaagc acgcaaaact tttgaccatg
    16201 taccagaacg atttgcttca tttgcaaaga aataccagca agaattgatt ttcgaagaag
    16261 aaaagaagca agctattttt aaactcagtc aacttatgaa agaacatcca gtcattactt
    16321 ttgtttatgg tgcaaaaaat gaacatcaga accatgctgt tttattgaaa aaatttatcg
    16381 aaatggaggc atgcaaatga aaatagggat cattggtgcc ggacctagag gattaagtgt
    16441 agcagaaagg attctccata ataatcagga cagacattct cttgaacttt ttcttttcga
    16501 tcccaatggt cctggaggtc gagtttggcg gttgaatcag ccaccggagt tattaatgaa
    16561 ttctgtttct caacaagtga cgctttttac agatgaaaca ttgtccagtg gaggattggt
    16621 aacacaggga cccaacttgt ttcaatggag taaaggacct gctacagatt atatcgaaca
    16681 gacggtaacc aaaaataagc aactaattat agaagaaatc aatcgtctgg aaaaagatca
    16741 gcaatctact cgatgtttat acggactcta tcaacgctgg tttttctcaa agttgcaaga
    16801 agaatttcca gcttctattc agtttgttta ttctttagta aaaaaagtaa gcaaaagaaa
    16861 agatggttat cttttacaga cagatcagca aagctattct atcgatcagc ttgttttagc
    16921 aagcggtcac tgggaaaatg atgagacaga agaagaaaga gtgtgggtag actacgcaaa
    16981 gaaaaatgtt ttactgtatc agcttccttc taatcctgca gatgtagatc tttcgaagat
    17041 accgcctaaa gaaaatgtct ttttaagagg tctaggctta agtttttttg attatgtcgg
    17101 attgtttaca gtatcaagag gagggcgctt cgagaacgaa aagggaaaac tggtctatca
    17161 tccatcaggc aaggaaccga ttgtatacag tggatcaaga agggggctcc cttattaccc
    17221 tcgaggaaga aatcaaaagc aaggcggagc aatggcatgg ccacggttac taacaaaaga
    17281 aaacctggag caatggcatc agtcgggatt tttgactggc gaaacatttt ttgactatct
    17341 gaaaaaagac gttgagttgt tttattacaa gaaaatcatc gaagaatatc acttgccgat
    17401 tccaaagaaa aattttgagc atgattttct ctccctctct tcagaggagt gtctcggaaa
    17461 gcatccagca ctaatccctt atagatggga ttggtcgtat ttagaaactc ctcttgccta
    17521 tcagaatgag cccttttcag aagctagccg aatatttatt gaagggcaga taaaagaagc
    17581 ggaaaaaggc aattgcacag gagcatttgc ttcaacattt gatgcattaa aagactggcg
    17641 tgatccaatc cggcaggcta ttgaatggca cgttttttct gctaaggaat acaaagaaat
    17701 actatgggga tggtttactc atctaaatag ctttttaacg atcggtccgc caatcattcg
    17761 gacgaaagaa ttagcagcac tgatagatgc gggtattttt catttgatcg aaccgccatt
    17821 tgatattcag caaaagcaca accgctatgt tatttctcta caagataaag aaatcagcag
    17881 tcgctatttg attgaagctc gattgccaca aacaagcttt tctcgttcaa gtaatcctgt
    17941 cataaaaaat ttgaaggaaa acaaacttgt tcgccctttt tcattttcag ataatacagg
    18001 aagttatcat tcagaagcta tagatgttga gcgaggtacg aatcgcgtga ttgatgcttc
    18061 aggggaaatc cagtctgact tttattgcta tggaatacct gttgaaggtg tggattggct
    18121 aactgcagca gtagctagac cttatacaga tccatggaat ctgagacagg cagataaaat
    18181 agcacaactt atattgactg acaaataaaa atacgcatga tgacttatct gttttttaga
    18241 aagtcttcat gcgtattttt aaataattat taacgagaaa cgcgtttacg cattgctttt
    18301 ttacggtttt cttcgatcat tgcttctttt tcgtcaattg gatcgatcac tgtttttttc
    18361 acagaagcaa ccaaaccagc aacggcagcg gctgttgcca gacttcctac taatattcca
    18421 gatacaaatt ttttcattga atccacctca tttattattt gttatatcta ttatggaaca
    18481 aaagagaaaa aaaggctagt tttacgtttg tttgtaaacc tttgcaaaaa atccttgaaa
    18541 atgtttacta aatgttttat tataaattta ccatatgaat ttacgaaatt ttactttgtt
    18601 tgttttcacc ataaaagtaa acttcgtttt ttaaattata tagaatcaac aaacacaatt
    18661 ttactaatca aaggagtgtt ttttatgaca attttagtac taggcggagc cggttatatc
    18721 ggttcgcacg ctgtagacca gctagttcaa aaggggtatc aagtagctgt ggtagataat
    18781 ttgctcacag gtcacaaaca agcagtacat ccagatgctc atttttatga aggagacatc
    18841 cgggataaag aatttctacg ttccgtattt gaaaaggaac cgattgaagg agtaatccat
    18901 tttgctgcaa gctctttagt aggagaatct gtcgaaaaac cattgatgta tttcaacaac
    18961 aacgtatacg gtatgcaaat cttacttgaa gtcatgcacg agttcaacgt gaataaaatc
    19021 gtattctctt ctactgcagc aacttatgga gagccgaaag agtctccaat cacagaagat
    19081 acaccagcta atcctaaaaa tccgtatggt gaaagtaaat tgatgatgga aaaaatgatg
    19141 aagtggtgtg accaagcata tggcatgcgc tatgtcgctt tgcgctactt caatgtagcc
    19201 ggagcaaaag cagatgcatc tatcggagaa gatcatacac cagaaacgca tcttgtccca
    19261 atcattttac aagtagctct tggtcaaaga aaagccttgg ctgtttatgg agacgattat
    19321 gatacaccag atggaacatg tatcagagat tatgttcaag tagaagattt gattgctgca
    19381 catattttag ctttggaata cttgaaagaa ggaaatgaaa gcaacttctt caatttaggc
    19441 agcagcaaag gatactcagt caaggaaatg ctagaagcag ctagagaagt gacaggaaaa
    19501 gaaattccag cagaaatagc accaagaaga gctggagatc ctagccgatt agtcgcttca
    19561 agtgaaaaag cgagagagat tctgggctgg aaacctgaat acacagatat caaagcgatt
    19621 atcaaaacag catgggattg gcatgtcagc catccaaatg gttatgaaga ataggggaat
    19681 gagatatgtc aatcagtcaa acgatttgtg attttgccac acttgcgatc gaagctggcg
    19741 gatggatgga aatggaccgc ttatatttac agaatcgtct tttagggatg gtaggagaag
    19801 atgctctaga aagcattgaa gtaagaccag ttaaggaaga atctgtagac ttattggatc
    19861 agcttgtcgc taaggcaaga gaaaatggtg ttctttcaga attgccagca gaacaagaga
    19921 tttttgaagc gcaattaatg gattttctca caccgccacc ttcagtcgtc aatgcattct
    19981 ttgcacagca ctattcgaaa agcccagagg aagcaactga gtatttcttt aatttaagta
    20041 agaaaaatga ctacatcaaa acaagagcca tcgcaaaaaa catcaaattt ccggcagagt
    20101 cggagtatgg tgaactggaa atcacaatca atttatctaa gccagaaaaa gatccaaaac
    20161 aaatcgcagc agaacgcgag gcggtaaaag tagactatcc tacctgcatg ctttgcatgg
    20221 aaaacgaagg atatagaggt cgcttgaact atccagcccg caccaatcac cggattattc
    20281 gaatgaatct agacggtgaa agctggggat tccaatattc cccttatgct tattacaatg
    20341 aacattcgat cattttatca gaacaacacc gcccgatgaa aatcacaaag gaaacatttg
    20401 ctagactgct tcggattact gaagtcttgc ctcattactt tgttggttcg aatgctgact
    20461 taccaattgt tggaggatcg attctttctc atgatcatta tcaggcagga cgtcatgaat
    20521 ttcctatggc aaaagcaccg atagaaaaac ttatcaaact agaagattat ccgcaagtca
    20581 tcgcaggagt cgtgaaatgg ccgatgtctg tgatccgttt gcaatcaaca gagaaaaact
    20641 tgcttattga agcagctaac tcagtattgg aaaaatggaa agcatattcg gatgaaacag
    20701 tatctatcag agctttttca gcagatggta caccacacca tacgattacg ccaattgcac
    20761 gaagaaggga caattacttt gagttggacc ttgtccttcg agacaacaat gtttcagaag
    20821 aacatcctga tgggattttt catccacata aagatgtcca acatatcaaa aaggagaaca
    20881 ttgggttgat cgaagtaatg gggcttgctg ttttaccacc tcgtttgaaa ccagaactag
    20941 tagaagtaga gcatttctta ttaggagaag aaaaccagat ggctgaatat catcgtccgt
    21001 gggcagaaca attaaaaaaa gaacatcctg atttgacgaa agatacggtc accgatgtcg
    21061 tacaaaaatc tgtaggtcag gtttttgcac gtgtactgga agacgctgga gtattcaagc
    21121 gagatgaaaa aggacaggca gcgtttgaac gatttactgc gattttataa acgtaaaaaa
    21181 ggaggagaga caaatggcaa caattaaaga cattgcgcag ttggcaggag tttctcctgc
    21241 tactgtttca agagttctca actatgatcc tgatctttcc gttggcttag ccacaaagca
    21301 aaaaattttt gaagcagcag aacgcttaaa ttatacgaaa catcgtaaaa atgcagcaaa
    21361 tactaagcaa acgcttcgcc tgatccagtg gtacgacgat acggaagagc ttgaagatct
    21421 ttattattta tctattcgcc taggaatcga aaaaaaggca gaagaagcaa acgttacttt
    21481 attgaaagaa acgtgggatt ccattagtga tcttccggca gatggaacga tcgcactagg
    21541 aaaattcgac gaagctcagt tattagaaat gaaaggatcc caaaatgcct tgcttttggt
    21601 agattctgac ggcacttctc aaggaatcga ctctctagtt gtcgacttta agtcaagtgt
    21661 tcaaaaagtc attgaacact ttgtctccca taaagcgcaa catatcgcta tgcttgctgg
    21721 aacagaatat acgaagaaaa accatcagcg gctaaaagat cccagaatta tggcatttca
    21781 acaagttttg gcagaaaaag gctacgacga aggtccaatt atcgaagcag atttttctgt
    21841 cgagtcagga tatcaagcag tgaagcagta tttaaaaacg aatccgcatg ttcctgatgc
    21901 gctttttgca gctaatgatg cattagcgat tggcgcactg aaagcgattc aagaaaaagg
    21961 tttagtggta ccagaagata tttctgtgat gggcttcaat gatgtcagtg tagcgaaata
    22021 tgtaacgccc tcgctttcta ctgtaaaagt ttataccgaa tggatgggag agctagcagt
    22081 ggagacgatt cttcaattaa gtaattccaa agcaccggtt tcaagaaaga ttattgtgga
    22141 aacagagttg attattcgag aatcaacgaa ataaaagatg gaaaagctgt gggaaaatgg
    22201 agtgataccg cagctttttt gtgtttcagg aaggggcaaa atttgcccgc cagcagataa
    22261 tcatgtatta ttgtactagt tttgcctttg gtattttcat cctcagaata aaaaagtatg
    22321 ctacaatgga aggcgagaat gtcatagcag aaaggatcaa atatatgatt acattaggaa
    22381 tagatactgc aaaccaaact ttggcgatcg gtgtggtaga agacgaacaa attcttggac
    22441 aaatacaaac aaatatcaaa cgcaaccaca gtgtaacact gatgcctgca atcgaccaat
    22501 tgtttgcaga tcttcaaata agtccaaaag acattgaccg aatcgcggtt tctgatggtc
    22561 ccggttctta tacagggctt cgtataggtg taacgacagc aaaaacgatt gcttatacat
    22621 tagataaaga gctagtagga gtatctagtc tgaaaaccat cgcggctaat tgcatcggtg
    22681 tggataatat tattgtgccc atgtttgatg ctcgaagaaa aaatgtatac gctggggcat
    22741 accgctggaa agaaaataga ttagaaactg tacttgaaga tgtacatatt tctgcttctg
    22801 atttgttcac acaactacaa aagctgaatg aagctattta tttcgtcggg agtgattgtc
    22861 acaaattctt ctctgaaata aaggaaatat tgcctagtgc gatgattaat acagttccag
    22921 catgggatat tccaagtggt gtaactgtgg ctcaattagg tgctttagtt gaaccagttg
    22981 cagagaaaca agcttttctt cctcgctatt taaaacgagt agaagcagaa gaaaaatggt
    23041 tggagacgca cacacctgga gacgaaagtt atgttgaaaa aatttagagc gtatgccaga
    23101 agcttttttc aagataaaat gatttataca gaaaaaacag tcgaacaaat cgatcgtcgt
    23161 tacgtactaa gagaattgac tacagaagat atcaaagagc tgctggatgt cgaacgagaa
    23221 gtctataatg gcgaattgcc atggacaaaa agtgccttta tatcagaatt gaagtcaccg
    23281 cttcctcatc tatatctagg tatctttgat aatggaagaa tgattggttt tatcggttca
    23341 agagcgttcg gtttagattg ccatatcacg aatattgccg ttcgaccggc atatcagcgt
    23401 aaacgaatcg gttcgctgct tattgacgaa atcgagaact ttgctataat gaatcgctgt
    23461 gagacgatgt cattagaagt gcgtatgagt aatcaagatg cacaaagact ttatcggaaa
    23521 ttggggtttc aagcaacaac gatcaaaaaa agatattaca ctgaaacaaa agaagatgcg
    23581 ttggatatga taaaactttt agaaaatgag tgagattagt tgatttgcag aaaaaaagat
    23641 tttgatgaag cgtacttggc agaatgcgta tggatgatta gcgaaaatgc ttatccacat
    23701 ggttcacctt ggacgaaaat gcagtttttg gcagacatcc aacagcctca ctctgattat
    23761 ctgcttttag ctgaagataa ccagatctgt ggctttattg gatactcgaa agtaatagat
    23821 gaagtcgaga tcacaaatat tgctgttgca gtttcagaac aaggaaaagg gcatgcacgg
    23881 catttgttac aattactgat tacggaacaa ataaaagatt cgctgcatgt ttttttagaa
    23941 gtacgtctat ctaatgaagc agcaagaaaa ctttatgaat cagaaaagtt tcgtatatta
    24001 ggtaaacgga aatcatatta tcgcaatccg acggaagatg cgatcattat gagcagaaag
    24061 ttaaaaacgg agacgatgaa atgaatgaag aattgattct ggcgattgaa tcaagttgtg
    24121 atgaaacgag tgtagcggtc gttagaaatg gaacagagat cttatctaac atcgttgctt
    24181 ctcaaataaa cagtcataaa cgttttggtg gcgtagtacc tgaagtagct agccgccatc
    24241 acgtagaaca aatcacctta tgcttggaag atgcactggt agaagctggt gtgtcggcag
    24301 aagatttatc ggcagtcgct gtcacttatg gaccaggact tgtcggctcg ttgctgattg
    24361 gtatttctgc agctaaggct tttgcttggg cacaccaatt gccgcttatt cctgtaaacc
    24421 atatggcagg gcatatctat gcagcccgtc tggttaaacc attccagttt ccattgatgg
    24481 cacttcttgt tagcggagga catacagaat tagtctatat gcaagaagat ggatcatatg
    24541 aaatcattgg ggagacaaga gatgacgcag caggagaagc ttatgacaaa gttggaagag
    24601 tattaggtct ttcttaccca agcggaaaag aaatcgatca gctagctcat cagggaaaag
    24661 ataattacca ttttccgcga gcgatgatcc atgaagataa ttatgatttc agttttagcg
    24721 gattaaaaag tgcatttatc aatttggtgc ataatgcaca acagcgtgga gaagatctag
    24781 acaaaaatga tttagcagct agctttcaag caagtgtgat cgatgtattg attactaaaa
    24841 cacttcgagc ttgtcaaaat tatccagtca agcaactgat tatagctgga ggtgtagcgg
    24901 ccaatcaagg gttgcgtgaa ggattgcagg atgctttggc aactaaactc cctgaggtag
    24961 aactagtgat tccccctctt cgactttgtg gagataatgc tgcaatgatt ggagcagcag
    25021 cacatgtgga aatgcaaaaa agaaattttg ctagctatca gttgaatgct gatccaagct
    25081 tggttttatc tgaataaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
    25141 cccaggaaaa acggcttctc atgtttttga cctggctgtt cttattaccg cgtatgcaag
    25201 cttttgaacg ccgtttaatc aagagttcgt aagcctgtcg tttaactctg attcacaagg
    25261 aacaatttca aaaaacagct cctcatgttt atgaacatta tgacttgtga ccgaagagtt
    25321 gacaggtaca caccgtttaa tcgaaatagg gctgtgacac gtttttgtca aagccctatt
    25381 ttttacatac ttttaatcat tattgttatt ttgctatttt ttacagctta tttatcatta
    25441 tccgttcctc tgattgccct cactgagtaa tcgtttattg tcgaggaata tcgaataatc
    25501 tagatcggat gaggacaaga cgcttcatct agttaatcta acagtatttt ttgcaaacag
    25561 tgaatattac ataactgaaa cacacataga aaatctttga gtcttgtcca gtgatctaaa
    25621 actgccggat ctttcagaag ttttagggag gaaaataagc agtgaccctg atattt
//
LOCUS       DACECR010000036        25555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.036, whole genome shotgun sequence.
ACCESSION   DACECR010000036 DACECR010000000
VERSION     DACECR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.036"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(136..843)
                     /gene="deoD"
                     /locus_tag="IWO17_001343"
     CDS             complement(136..843)
                     /gene="deoD"
                     /locus_tag="IWO17_001343"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0290227.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(860..1678)
                     /locus_tag="IWO17_001344"
     CDS             complement(860..1678)
                     /locus_tag="IWO17_001344"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0290228.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IWO17_001345"
     CDS             complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IWO17_001345"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0290229.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(2967..3923)
                     /locus_tag="IWO17_001346"
     CDS             complement(2967..3923)
                     /locus_tag="IWO17_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0290230.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3923..5056)
                     /locus_tag="IWO17_001347"
     CDS             complement(3923..5056)
                     /locus_tag="IWO17_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0290231.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5049..6611)
                     /locus_tag="IWO17_001348"
     CDS             complement(5049..6611)
                     /locus_tag="IWO17_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0290232.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6823..7905)
                     /locus_tag="IWO17_001349"
     CDS             complement(6823..7905)
                     /locus_tag="IWO17_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0290233.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(7971..8369)
                     /locus_tag="IWO17_001350"
     CDS             complement(7971..8369)
                     /locus_tag="IWO17_001350"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0290234.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IWO17_001351"
     CDS             complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IWO17_001351"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0290235.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9098..10399)
                     /locus_tag="IWO17_001352"
     CDS             complement(9098..10399)
                     /locus_tag="IWO17_001352"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0290236.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10540..11556)
                     /locus_tag="IWO17_001353"
     CDS             complement(10540..11556)
                     /locus_tag="IWO17_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0290237.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11864..12469)
                     /locus_tag="IWO17_001354"
     CDS             complement(11864..12469)
                     /locus_tag="IWO17_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0290238.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12665..13588
                     /locus_tag="IWO17_001355"
     CDS             12665..13588
                     /locus_tag="IWO17_001355"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0290239.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13647..15212
                     /gene="guaA"
                     /locus_tag="IWO17_001356"
     CDS             13647..15212
                     /gene="guaA"
                     /locus_tag="IWO17_001356"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0290240.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15703..17334)
                     /locus_tag="IWO17_001357"
     CDS             complement(15703..17334)
                     /locus_tag="IWO17_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ0290241.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(17348..19021)
                     /locus_tag="IWO17_001358"
     CDS             complement(17348..19021)
                     /locus_tag="IWO17_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0290242.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19034..19351)
                     /locus_tag="IWO17_001359"
     CDS             complement(19034..19351)
                     /locus_tag="IWO17_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0290243.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="IWO17_001360"
     CDS             complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="IWO17_001360"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0290244.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21013..21798
                     /locus_tag="IWO17_001361"
     CDS             21013..21798
                     /locus_tag="IWO17_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0290245.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            21801..22493
                     /locus_tag="IWO17_001362"
     CDS             21801..22493
                     /locus_tag="IWO17_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0290246.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(22767..23138)
                     /locus_tag="IWO17_001363"
     CDS             complement(22767..23138)
                     /locus_tag="IWO17_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0290247.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(23538..23768)
                     /locus_tag="IWO17_001364"
     CDS             complement(23538..23768)
                     /locus_tag="IWO17_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290248.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(23862..24200)
                     /locus_tag="IWO17_001365"
     CDS             complement(23862..24200)
                     /locus_tag="IWO17_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290249.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(25128..25244)
                     /locus_tag="IWO17_001366"
     CDS             complement(25128..25244)
                     /locus_tag="IWO17_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0290250.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         7453 a         5609 c         4509 g         7984 t
ORIGIN      
        1 ttcacaacct cttggtaaac ggtgttcaaa agcttacatt tgcaataata agtccagcca
       61 agtcaaaaac atgaggagct atttttgctt ggatgttctt attacttaat gcagggcagc
      121 tttttcattt ccttcttatt ctgctatcgc tgtttccaat cctacttcga tcatttcatt
      181 gaacgtagat tgacgttctg cagctgttgt ttcttcaccg gtgatcaaat ggtcgctaac
      241 ggtcatcaaa cttaatgtct gcacatggta tttcgcgcct aagtaataaa gagccgctgc
      301 ttccatttcc actcctaaaa cgccaagttc agctaattgg aacgtctctg tcaaatcatc
      361 tttgtagaaa gaatcttccg aaagaacatt tcctacatga accgtatagc ccttttcttt
      421 agctatctca tatgctttca gtaatagatt gaaatcactg attggaggaa aatgaaaggc
      481 ttggaaattt ttttcgatca tcgaagaact ggtcgctgca ccttgtgcta gtactaaatc
      541 acgaacatgg acgtcttttg acaatgcacc gcatgtacct actcgaatca atttctttac
      601 accatacgac tggatcaatt catgggcata gatggtggct gatggcattc ccattcctgt
      661 tccttgaaca gaaatacgtt ctcctttata tttgccggta aagcctaaca taccgcgtac
      721 ctgattgtaa caaactggat cttccaaaaa agtctccgca atatatttag ctcgcaaagg
      781 atctcccgga agtaaaatct tatcggcgat ctcgccttct ttcgcttcaa tatgcacact
      841 cattttattg ttcctcctat tacaataatg tcaatgtttc tttaaccaat gctttaaaag
      901 attgtttgac tctctgagtt gtttctacaa cttcttcatg gctcagattc gcttgcatac
      961 ctgctgctaa gttggtaata caagagattc ctaacacttt taatccgctg tgagctgcta
     1021 cgatgacttc tggaacagtc gacatcccta ctgcatcggc tcctattgtc cgagacattc
     1081 ggatctccgc aggcgtttca taagttggtc cagaatagcc catatatacg ccttctttaa
     1141 gatccaaacc tagtttttct gctgctgctt ttgctgttgc ttgatagtca ggcgtataag
     1201 cttgactcat atctggaaat ctagggccca ttgcatcttc attcggaccg atcaaaggat
     1261 tttcaccagt aaaattgata tgatcggtaa tcagcatcaa atctcctggt acaaatgatt
     1321 cgttgacacc accacatgca ttagtcacaa tgactgaatg ggcccctagt ccggccatca
     1381 cacgtactgg ataggtcact gtttgcatgg aatgaccttc atagtagtgg aatctccctt
     1441 gcatggctaa cactttcttg cctgccagag acccataaac tagctttcct gcatgcccaa
     1501 ctactgtcga aacaggaaag aacgggatct catcataaga aatcattaca gcatcttcga
     1561 tttcatctgc taattcacct aatcctgaac ctagaatcaa accaaaatca atttctttta
     1621 ctcctttatc aaggataaac tgggtcgttt cttgtaacat ctcgcttaac ttggtcattt
     1681 gctttctcct atttaaaaag attattttaa taatttcaag aagctttcgc cattttcagt
     1741 cgaagaaaca ctaaagttct cagcaatcgt tgctgaaata tctgaataat atccttgagg
     1801 caatgcccct tgttctttca tttttttact gtaaaccaat aacggtacat attctcgcgt
     1861 atgatcggta ccagtaaacg tcggatcgtt tccgtgatcg gctgtaatca ttagcaagtc
     1921 gtcttcttcc atcgcttcaa gcaattctgg aattcgtaag tcaaattctt caatcgcatg
     1981 cgcatagccg actacatccc gacgatgacc gtataatgca tcaaaatcta ctaaattggt
     2041 aaagctgatt cctgaaaaat cgtttttcat aactttcagc agctggtcaa cgccatccat
     2101 attgcttttc gtgcggacag attcagtgat tccttgtccg ttgaaaatat cgttgatctt
     2161 tccaacagca atcacgtcct ttcctgcttc tttcaaagaa tcaaggactg tgtgtccgaa
     2221 tggattcaac gcataatcat ggcgattgct tgttcgtgtg aagtctcctg gttcacccac
     2281 gtacggacgt gcaatgattc ggccaatcat atacggatca tcttttgtta tatcacgaac
     2341 gaattggcaa atccgatata attcttctaa aggaatgatt tcttcatgtg ccgcgatctg
     2401 aaggacagga tcggcagaag tatatacaat taaatcgcct gttttcattt ggtgttcacc
     2461 gtaatcgtca ataacagctg ttccgctata aggtttgtta catacgactt tacggccaga
     2521 aaattcttcg atttgtttga tcagatcatc agggaatcca ttaggaaaca cacgaaatgg
     2581 agttttgata ttcaatccca tgatttccca gtggcctgtc atcgtatctt tccctacaga
     2641 aacttcttct aatttcgtcg catagccttg atgatctttc actgattcca caccttcaag
     2701 aggacggatc gttcctaaac caagctgctc taaatgtgga acgttcaatc ctgcttcttt
     2761 cgcaatgtgt cctaatgtat cagaaccaac atcaccgaac ttatcggcat ctggtgcttc
     2821 tccaattcct actgaatcca tgacgatcag atgtacgcgt ttaaacatga ttattccttc
     2881 tttcttttta tttttattgc ctttaaaaca aaaagggcga gacgaaaaac acttcccgta
     2941 acccaccctt tttattcgct aaaagattat tttgatttga tatagttctt cccgtttgct
     3001 tttggaccag aagctttccc taagaaaaca accagcacaa tgattgtcag caaatatgga
     3061 gcaatttgca gcaatactgg cggaatcgaa gaaataactg gtaattgtgc gccaatgata
     3121 ctcaagcttt gagcaaaacc aaagaacaat gaggcaccca ttgcccctaa aggattccac
     3181 ttaccaaaga tcatagcagc catggagatg aacccttgtc caacgatcgt accagctgaa
     3241 aagttaccgg aaatcgactg agcaaaaaca gcaccgccca taccgcctaa caagccagaa
     3301 atcagtacgc ctgaataacg catagcatat acattgatac ctaatgtgtc ggcagcttga
     3361 ggattttcac caactgaacg gaggcgcaag ccgaatttcg tcttgaagat gatcacccaa
     3421 gctaggacag caatcaaaat agcacaataa gcaggtaaga atgtattttt aaagaaaatt
     3481 ggtccaatga ccggaatatc tgctaatcct gggaaagaaa aataccccag attctgcgtg
     3541 atgttatcag tttgaccttt accataccat gctttgatca agaaaacacc taaagcaggt
     3601 gccatcagat taattactgt cccgctgata atatggtcag cacgcaaact gactgttgct
     3661 actgcgtgaa tcagagaaaa taggatacct acgacgccac ccactaaaat agcgatccat
     3721 ggtgtcatag aaccaagtga tggactcatc gtcaagttga aaacgatgga actaaaagca
     3781 cccatgacca tgattccctc tagccctacg tttacgatcc cgcttcgttc agaaaaagtt
     3841 ccgcctagtg acgttagaat tagcggtgtt gaataaacca gtgtagaagt aaggatcaag
     3901 gcaattaatt cgatagtcga cattatagtt cgcccccttc atggttttga ttcgttggtt
     3961 tcgattctat tgtcgcaacg atctcttctt gttttttccc ttggaagact ttagccatga
     4021 cgaaacgaat caggaagttg atcccgacaa agaagataat caaagcaatc gatacattga
     4081 cgatctcaaa cggaacacct gctcctgtct gcatgccaag tccaccgatc ttcaaaatac
     4141 tgaacaagat cgctgataaa agaataccga ttgctgatcc agaaccaagc aaggaaacag
     4201 ccattccgtc aaatccaata gatagcgaag tcgtttgaac aaagaagttt tgatacgttc
     4261 ctaatccttc aaccacaccg ccaagccctg ccaaagcgcc agaaatgacc atcgacaaaa
     4321 cgatcgtacg tttactactc attcctgcat actcagaagc aaaaggattc aatccaacag
     4381 aacggatttc aaatcctaaa gtcgttttct tcatcagcag ccaaacgaaa ataagcgcga
     4441 tgattgctaa aaataatcct gagtttacgc gagagccgcc aaaagcttgt gttaaccatg
     4501 gtaaccgcag tgaggcattt tgactgatca tattggaaga gtccaaactt gagcgatagc
     4561 tttcaggcat cacttcatgt aaagcaaatg tgcttacgta taataagata tagttcatca
     4621 tgattgttac gatcacttca cttgttccaa aaaatgctcg aagtaaacct ggaacagccg
     4681 ctgccatcgc accagctaag gcacctaaga tcacgcataa cggaagcaaa acaacttttg
     4741 gcagatctgg aaacgcaagt gccacccaga cactgacgat ccacccgcaa agtgcttgtc
     4801 cagaaagccc aatattgaaa aacccagctg agttagctac agaaaaacct aatgctgtaa
     4861 aaatcaacgg cgttgcttgc cttaacgttt cacctatccc tctttgcgaa ccaagggctg
     4921 catctagcat agaagaataa ccttggatgg gctgaaaacc aaaagcagcc atcaatatcg
     4981 cgcctaagat caatcctaaa acaactgaaa gtacaggcac taataaatta cggaattttt
     5041 cgctgcgatc attcatgtgc gccagccccc ttttctaact cttttcgtgc ttcttctaag
     5101 gtatagcccg ccatcaatag acctaattca ttttcagatg tttctttcgg atcaacgata
     5161 ccgacaattt ctccttcatg gataacagca atacgatccg aaacatttaa aatttcttct
     5221 aattcgaaac tgatcagtaa cactgctttg tatttgtccc tttgttcgat cagacgttta
     5281 tgaataaatt cgatcgcacc tacatccagt ccacgagttg gatttgcaac aatcaacaga
     5341 tcaggatctc ggtcgacttc acgagcgata attgcttttt gctgatttcc gcctgataaa
     5401 gcttttgcgg gaacaagttc attcgttgtt cgaacatcat attcttcgat caattctctg
     5461 gcatgttcat tcattactga ataattcagc atgccgtttt tactgtaagg tttttgatga
     5521 taggtttgta gtgcgatatt ttcagataat gtcatatcta ggactaaccc gtatttatga
     5581 cggtcttctg gcacgtgtcc tacaccatgt tcagtgattt ttcggggttt tttattagtg
     5641 atgtcttccc cttttagctt gatatgtccg ctttctgcct ttcgcaaacc agtcaaagct
     5701 tggatcaact ccgactgccc gtttccatcg attccagcga taccaagtac ttcgccagca
     5761 cgaacctcta agttcaagtt tttcacggct tctaatccac gattttcttt caccactaga
     5821 ttttcaatag acaaaacgac ttcttgaggc ttcgcttctt ttttcatcgt tttgaatgaa
     5881 accgttcttc caaccatcat atcggctaat tgttgtgagg ttacgtcttt aacgttgact
     5941 gtaccgattc cttttccacg gcggataact gtacagcggt cagctactgc tttgatttca
     6001 tctaacttat gcgtaatcaa aatgatcgac ttgccttctt tgactaattc cttcatgatc
     6061 acgattaatt catcaatttc ttgaggggtc aatacagctg tcggctcatc aaaaatcagt
     6121 acatctgctc ctcggtaaag tgtttttaaa atttctaccc gttgttccat tccaactgaa
     6181 atatcacgaa cgtaagcatc tgggttgact gacaatccat actgttcaga aactttttgg
     6241 atctcttttc gtgctttttt atggtcaagc atccctgcgc gactaggctc gcttcccaac
     6301 acgatgtttt ctgttacagt aaaggcatca acaagcataa agtgctgatg gaccatccca
     6361 atccctaatt ggttggcttt ggtcggactt gtgatcttta cttctttccc acgcatcaaa
     6421 attttccctg atgtcggctc taataatccg gaaagcacgt tcatcaatgt agatttgcca
     6481 gcaccgtttt ctccaagcaa cgcatgaatt tctcctgcct ttacttgtaa attgatgttg
     6541 tcatttgcct tgaatgttcc aaattctttg gtaatattct gcatttctat gacaatattt
     6601 tcttgaacca cctttttttc acatcctaac gatttctatc tagggcaacg attgactaag
     6661 attcacgctt agtgagcgtt cttcttttga aacaaacatt acttcaggga gttgttccct
     6721 tatccaaact gtcacaaggc agcttggata aatgaacaat tcttgttcta atcaaaccat
     6781 agccttgtca aacatgtgtc taacaaggct atagaaatgg ttttagtttt cttcaggagt
     6841 ttctggaact tttacgtcac ctgaaatgat tttttctttt gcttcatcaa ctgctttttt
     6901 cgcttcgtca gaaagttgtc cttctgttag accaacaccg tcttctttca atccataaac
     6961 agtgtgttcg ccgccaggga atttaccgtc cgctgatttt tgtgccaaat cttctactac
     7021 tgttccaact gctttcagtg tagatgtcaa tgtgaagttt ttcttttcgc cgtttaatgt
     7081 gtattcgcct tcgtctgatt ggtcgcggtc aacaccgata acccaaactt ttttgtcgcc
     7141 tgattcgttc aatgattttg cttcttggaa tacaccgtta cctgtaccgc ctgaagcatg
     7201 gaagatgata tctgcgtttt gtgcatacat accttgagca attgaacgtc ctttatctgg
     7261 agcagagaag tcaccagcat attgattcaa tactttgatt tcttttccta attctttcgc
     7321 acctgcatca acaccggctt tgaatccagc atcaaaacga tcgattactt caccttttac
     7381 accgccgata aagccgacta cgtttgtttc agtcgtataa gctgctgcaa cacctgcaag
     7441 ataagaagct tcgttgtctt tgaaagttgc agatacaacg ttatctaatc catcgatgac
     7501 atcatcgata ataacaaagt tcgtacctgt attgtttgtt gcttgttctt ggatcgctgg
     7561 ttttagttta taaccaatac caaaaatcgt tttgaatcca gcattcaatg cttggtcaat
     7621 atttgggatg taatctgatt catttgaaga ttggaaatat tggaagccat catttcctcg
     7681 tgaaagtcct tgatctttcc cccatttttc tagtccttcc caagctgact ggttgaatga
     7741 acggtcgtct acaccacctg tgtctgtgat caatgctgca gttgttgtgt ctgatgagct
     7801 tttgccaccg gatgaatcag tagagccgtt gttgccgcat gcacctaaaa ataaccctgc
     7861 tgctagtgcg acagcaccta aaccaaataa ttttgctttt ttcatttctg taaagcctcc
     7921 taaaataatt gttttatttt tcaacagtca gctgactgaa tgaacatgag ttatagatct
     7981 ttttcagtaa atgcataagg cagcagtgca cgaacggtcg ttgccttcgt cacaccattg
     8041 tctcctacta gcgtaaccgg catatcaggg gaacagaatt ctgccattac ttgtcgacat
     8101 gcaccacaag gagagatagg atcgggggtg tgccccgcta cgactagatg cgtaaatgtt
     8161 ttttctcctt cagatactgc tttgaaaaaa gctgtgcgct cggcacaatt cgttaatcca
     8221 aatgatgcat tctcaatgtt gatgccttga taaatcttgc ctgattccgt tacgagacaa
     8281 gcgcctaccg gaaaatggga ataaggaaca taggctttat ctaacgcttc aatagctgtg
     8341 ttgatccatt cttgtttgag ttctgccatg agcttctcct ttgaaaaaat taatagcctt
     8401 caccagttga accagatacg attgctactc ctgcgcttgt tccgattctt gtagcaccag
     8461 cttcgatcat tgccattgct tcttcttcgt tatggatacc gccggaagct ttgacaccca
     8521 tttctggtcc tacagtttca cgcatcaaac ggacatcctc tacctttgct ccgcctgttg
     8581 agaaaccagt tgatgttttg acaaaatcag cgcctgcagc ttttgctatt tcgcaagctc
     8641 ggatgatttc ttctctatct aataacgatg tttcaataat aactttgaca agcgcacgat
     8701 cttttgctgc ttcaacaact gcttcgatat ctttttggac ttgttgatca tcctttgatt
     8761 tcaaagcacc gatattgatg accatatcga cttcatctgc accgttattg atggcatctg
     8821 ttgtttcaaa tgcttttgtc tcgggtgtgt ttgctcctag aggaaacccg attactgtac
     8881 atacggctac tggctcacct tgtaattgtt ctttcgctag cgcaacccat gctggattga
     8941 tgcagacaga ataaaaatgg tatttttttg cttcttcgat aatttgcatc acagcttctt
     9001 tagatgcgtc tgctttcaaa atagtatggt cgatcattcg gtttagttcc atttcttttc
     9061 cccttttctt taaaacggta aagtccgctt ttttccttta ttctgtgatg atgtcatgaa
     9121 tcaacgttgg cttcattgcc gattcaccaa tttcaatatt tttatataat aattcaatga
     9181 cagaagtgat ttcttcgtcg ttactataga tcgtcaatag tgactcacct tttgtcacag
     9241 tgtcaccaat ttttttatgc agtttcaatc caacagcgtg atcaatatct tcttctttgg
     9301 ttttacgccc agcacctagc atcattgcag caattcctaa ttcatttgcc acgatttttg
     9361 acacaactcc gctttgttta gcaggaagct ccattgtgta gcgtgcagtc aaaattcgtt
     9421 ctggatgttc cacgatcgct gggtctcccc cttgattttg gatcatttct ttgaattttg
     9481 ctaacgcttt acctgattcc agagcttctt gaagcaacgt tcttgcttca tcggttgtct
     9541 tagctttgcc agcaagaaca accatttggc ttcctaaggc gtagcacatt tctactagat
     9601 cttctggacc atttccttgt aatgtttcga ttgcctcgac aacttctaga ctatttccga
     9661 tagcttcgcc aagaggttct gacatatctg agataaccgc cactgtttct cggttagcca
     9721 gtttcccaat gctcgtcatg gtctttgcca gacgtcttgc atcttcgatg ttcttcatga
     9781 aagcaccgtc tccagtggtc acatccaaaa cgattgcatc tgctcctgcc gcgatttttt
     9841 tactcataat cgaactggca atcagcggaa gagaatcaac ggtcgctgtg acgtcacgca
     9901 aggcatacaa ttttttgtcc gccggtgcca agtttcctga ttgaccaatt accgctactt
     9961 tactttcatt aacaatgcgt acaaattctt catccgaaag ctcgatctga aatccgggaa
    10021 ttgcttctag cttgtctagt gtaccacctg tgtaacctag accgcgtcca gacattttag
    10081 ccactgtcac tccgacacta gctactaggg gggctaaaat caatgtggtc gtgtcaccaa
    10141 cgccacctgt cgaatgctta tctaccttta ttccttcaag agaactcaaa tcgatgacat
    10201 caccagaatt agcaatcgcc aatgttaagt tcgtgatttc ttcatttgtc atgtcttgat
    10261 aaaaaattgc catcaataat gcactgactt gataatctgg aatctttcca ttcgtatagt
    10321 tcgtgacgat gtactcgatt tcctctttcg acagctcgtt tccatccctc tttttttcaa
    10381 tcagatccac cattcgcatt gcctgatcct ccttgtcttc attcggtatt atacattatt
    10441 tttaggaaag gtaaaccact ttagtaaact ggtttactaa aaataaaaaa agcactaact
    10501 agcctttgta aatgcttaca taagcaatat accgccgagt tagccctttg tcaatacttt
    10561 tacgctctca cgaataatta attttgtttc gaacactttg ttcggaactt ttctttgagg
    10621 aaattcgatc gtatcgatca ggaaacgtgc agcataaaat ccaatatcaa aacttggctg
    10681 ctgcactgtc gtcaattgcg gaatcatgta ttctgacagc tttaatccat cgaatcctat
    10741 gactgagata tcttctggga tttttttgcc caattcatcg atcgcctgat agcatccgat
    10801 cgccatggca tcattcccgc aaaaaatagc cgtgatatct ttatgtttga gtaatttttt
    10861 tgatgcagta tatccacctt ggacagtcaa ctctccatgt tcaacaaatt tcccattaaa
    10921 aggcaggcca tgatctttca gcgcatgacg atatccgttg aaacgttctt ctaattgata
    10981 gtatccagtg gtttccttca gcatgccgat cttcgtatgg ccattttcaa taaacagcga
    11041 aacagcttga taggcgccct catattcctt tacgataagc cgtccgttat cccgacgatt
    11101 caatccgcga tcgattaaaa tgattggcat tttttgataa aaaccgctgt ctaatgcata
    11161 tttctcaggt aaaatatttg gtgtggccag caagattccg tccactgagc ggtgggaaag
    11221 ctcttgtaaa tacttgatct cattttcttg gctatgacga gaattgcata acatgatcac
    11281 atagcccaaa ggattcaaat agctctctac accttcgatc agtttggaga agaagaaatc
    11341 agtgacatca gggacgatca ttccgatggt ctttgaatgt cgattgatca catttgaagc
    11401 gaaataatcg ggcttataat gatgttcatt cacgatcgac aacacttttt ttcttgtctt
    11461 atcactaaat ctgcttcctt tgttattcaa aatctgtgag acagttgtta ctgatacgcc
    11521 tgccatttct gcgatatctc tgatggtcag cttcatccta ttggctccct tctatctaaa
    11581 ttccctatca gtgtaccaaa acccccaaga aaagaataga gaaaaaagga aagtatgttt
    11641 tttgataaaa acctccttta tctaccgact tggcagcctt ctatacaagt tctgtatctt
    11701 atttatttta tcgtaaaaaa caacgactct gttcctacta tttctttttg aagaaaaaat
    11761 agttctttgt ttttaaaaac ttctcaacaa aaaaagaact gatacgtacc cgtacatgca
    11821 cgcccagttc cactctttcg tcgctccact atttttcagc tagttattct ttcacacttt
    11881 tgatgatata atatccttta tcttttgtga cgatttctgc attaccaaat gtttcttcca
    11941 ttttcttttg cgcactcggc gctccttgtt tcttctggat cacaattgtc agtgtaccgc
    12001 ccttcttcaa ccgaggatat gcgcctgtca aaatttcatg tacaactttt tttccagcac
    12061 ggatcggcgg attgcttacg atagctgcat aggtttcttc atgaactgct tcatagatat
    12121 tcgattgatg gatatcaaca tgttcgattt ggttacgctt tgcgtttcct tgcgctagac
    12181 ttaccgctcg ttcattaatg tcgaccattt caacaaaccg ctgtgtcgca aatgccaaag
    12241 ctaacccaat cggtccatac ccgcatccca catctaagat ctttccttca tcaggtaact
    12301 cttcccaatc gaaagcatca atcaggacac gtgaaccata atccactgtt tcacgtgaaa
    12361 atacaccaga atctgttaca aattgaaagg ttctgccttt taactcaaaa gcccattgtt
    12421 caaaatcgtg cgccgtatct gggcgttcag aatagtaatg atttgtcatg tcttccatcc
    12481 ttctcatttc tttcatgaca acagtctatc cttttatgta tccgtgcgca agtcaagaat
    12541 aagactccgt ttctttctat tataaagatt atacaaaaag atcggcaatc tctcttaagt
    12601 ggtggggttt gcgtttattt tgtgttaaaa tagttccatt gatgggagaa cgcaggaggc
    12661 taatatgaat gatccaatga actactaccg tgtggcaaga gaagaatggc gagaatttta
    12721 tcgtaatggg caagcacctt tgacacaaga tgaactagat aatatcaaaa gtttgaatga
    12781 ccgcatttcc atgcaggatg ttcgagatat ctatgtacct cttgctcatc tgatttatct
    12841 ttatatgaaa gaatttgaat ctttgttctt gagtaaaggt ttatttcttc ataaatacgt
    12901 agaagtaccg ccttttatta tcgggatcgc cggaagtgta gccgttggaa aaagtacaac
    12961 agccagactg ctgcagacga ttttgtctcg taccttcaaa cgaagaaacg ttcagctgat
    13021 cacgactgac ggatttcttt atccaaatcg tatattaaaa gagcgtggaa tcatggaccg
    13081 taaaggattt cctgaaagtt atgatatgga gatgctgatc gatttcttga accgtgtaaa
    13141 atctggtcag gaagagataa aagtacctgt ttactcacat gatgtgtatg atatcattcc
    13201 agacaagtac gaattgatcc agcagcctga tatcttgatc gttgagggga tcaatacgct
    13261 tcaattacct gcgaatcagc aaatctacgt cagcgacttt tttgattttt ctgtctatgt
    13321 cgatgccgat cccgtgctga tcgaaaaatg gtatttggat cgttttggtg cgttactgga
    13381 tacggctttc caaaatccag agaattatta ttatcgtttt gctatcggag atcgtgaaga
    13441 agcctttaaa atggcaaaag atgtatggaa aaatgtgaat ttgaaaaatt tacaggaata
    13501 tatcttgcct acacgaggaa gagccgatat aattctccat aaaactgaaa atcatatcat
    13561 cgattcgatt ttcatgcgaa aatattagca ttttaaaata gtttgactat tttgagataa
    13621 aatttaaagg gataggggta ataaatttga caaacgttgc cgatatgacg aacgttgaaa
    13681 agattatcgt attagactac ggtagtcagt ataaccaatt gatcacaaga cgtattcgtg
    13741 atcttggagt cttttccgaa ttattgagcc accgcattac tgcagaagaa gtaaaagcga
    13801 tgaatccaaa gggaatcatt ctttctggcg gaccaaacag tgtatatgat gagaatgcat
    13861 ttgggatcga tcctgagatc ttcaatttag gtattcctgt attagggatt tgctatggta
    13921 tgcaattgat cacgtacaaa cttggcggtt ccgtagagcc tgctaaaaac cgcgaatatg
    13981 ggaaagcctt gttagaagta acaagcgatc aagctgtctt gtttaaagaa acaccaaaac
    14041 aacaaacagt atggatgagc catggtgact tggtaactaa agttcctgaa ggctttgaaa
    14101 cagtcggaac aagcaaagat tgtccgattg cttctatcca agacacaaca cgtaattttt
    14161 atggtatcca attccatgca gaagtacgtc attccgaata cggaatcgaa ttgctgcgcc
    14221 actttgcctt tgatgtttgt cactgtgaag gtaactggac aatggataac tttatcgata
    14281 tgcaagtaga aaaaatccgt gaacaagttg gtgacaaaaa agtcttgctt ggtctttcag
    14341 gtggcgttga ttctagtgtc gttggggttc ttttacaaaa agcgatcggt gaccaattga
    14401 catctatctt tgttgaccac ggtctgctac gcaaaggcga agcggaacaa gtaatggaaa
    14461 gtctaggcgg aaaattcggc ttgaacatca tcaaagtcaa tgcaagagat cgcttcttag
    14521 acaagttgaa aggtgtatct gacccagaac aaaaacgtaa aatcattggg aatgaattcg
    14581 tatatgtttt tgatgacgaa gcaacaaaac ttgctggtga agaaggcgtc tctttcctag
    14641 cacaaggaac gttgtatact gacgttatcg aatctggtac agaaacagca cagacgatca
    14701 aatctcacca taatgtaggt ggactgccag aagatatgca atttgaattg atcgaaccat
    14761 taaacacatt attcaaagac gaagtccgtg aattagggac tcagttaggc atgcctgatt
    14821 caatcgtttg gcgtcagcca ttccctggtc ctggtcttgg tattcgtgtt cttggcgaaa
    14881 tcactgaaga caagctagaa atcgtacgcg aatctgatgc tatcttgcgt gaagaaatcg
    14941 cagctgctgg acttgaccgt gatatctggc aatatttcac tgttcttcca gggatccgct
    15001 ctgtcggtgt tatgggtgac ggacgtactt acgactacac agtcggtatc cgtgctgtaa
    15061 cttcaatcga cggaatgacc gctgactttg cccgtatccc atgggaagtc ttgcaaaaaa
    15121 tctctgtacg tatcgtaaac gaagtagccc acgtcaaccg tatcgtgtat gacatcactt
    15181 ctaaaccacc agcaacagtg gagtgggaat aaagagccaa cataatatgg agggggaatc
    15241 cctataaatc aacattcttc aatagctaaa atgcttgatt ttcattaaaa ataagatggt
    15301 tccctaccaa aagagactat aatataagtt aatttgtttc attcttatca taaaaagaag
    15361 ttctcgaaaa acgaactgtt ttccgagaac ttttttctat ccatctaagt gctcaaatca
    15421 tattcacgac ggaattctaa ggttaccctt tcttaaacaa actttatgtt atctgcacaa
    15481 ctttttgcaa ttttttcatt gcttcttacg aaatcgttga actctcttta gcataataca
    15541 aagtgtagat gaagtctggt gctttactcg ttttggtaaa aatgactgga aatcgtttct
    15601 ggttattttt ggcaaaggcg aggtgcaggt tcgattcctg ccttctacgc taacgatgtg
    15661 gctgaaggga acctccgatc ttgaatcgaa acgatccgca gtctattgtt tcaccaatga
    15721 aacaaaatca acgttcgtcc aagcatttcc tgaagccttg ctataaaccc caatttcaca
    15781 aacacctgtt gtaattggaa tgtctttgat tgtaattttt gtccattcac ttgttttaac
    15841 aaccgcagta tttagttccg ctccaccata atttttgata tataactgat tagtatcaaa
    15901 ctctccccca gacattacat aggcactcaa tgtgtaagtt cctttgctta tgccactaac
    15961 attttgataa gctgatgcct cgtatgcttg atcctgccaa aaagttagtt tgtagtcccc
    16021 aaaaaaagtg cccgcttccg ttttgatcgt acccttagcg gttccttgtt gaaaccaggt
    16081 ttgccaattg gaaggactgg tcgttggtcc atcgctttcg aaacttgcat ttttaactag
    16141 atttatttgc gcccctgctt gtttaaacgc acgcaagctt ggcaaggcgt ttccagcaaa
    16201 atcaaacatc gcttggttgt cccattcgtt tcccatagtg gattgaacac tgagataatt
    16261 catacctgct tgtgttgccc aactcacatt tccgttgtac caaagaggtt cccaatagaa
    16321 gaagcccgcc ccgcgattat tcggtactgc ctgaatccga tcggcaagat ccagcaaata
    16381 ctctgcttgt cctgtcggtg ttggttcata tccccccgca gctgcttcgg cttcaccaaa
    16441 agcatttggc atagtatcgg cattcgctgt tgtatgccca taagctgttt caactacgat
    16501 cacttcttta ccataccgtt ggctgatcgc attcatattg gtttgaagtt cattcattga
    16561 accgtgccaa tacggataat acgacactcc aattaaatca aatgacacac ctcgactcgt
    16621 gatttcatca aaccaccaag taaatgctcc tgtatcgcca ccatcagcta agtggagcat
    16681 tactggagtt gttttaggtt gagtgtctcg aacagcttgg ataccagact tcaaaaaagc
    16741 agccaatcga tcaaattctc ccccgccttc tccccaagac ttgccataag gccagagcat
    16801 tccgctattc agttcattgc cgatctgaac catatcagga taaacgtcca gctgcttcat
    16861 ttccgacaga acgttctttg tatagtcgta caatgccgta ttcatttctt caaaggtcag
    16921 accttgccat gctttaggaa gattctgttt cccaggatca acccaaaaat cactgtagtg
    16981 aaaatccagc agcaccttaa gtcccagatt ttttgctcgt tttgcaaggt caatcgtcgt
    17041 ttccagatca tttgtccctg ctccataact ttctccatta ctattataag gatcctgcca
    17101 caaacgcagt cgaacatagt tgacaccatt attttttaaa atctctaagg catcttttga
    17161 aatatcgtct tcataatatt ttgcaccaga tttttccata tctgacagga tcgaaatatc
    17221 tgctccgcga ataaattcgc tggcagatgc ttcaacagtt tttgaaacag cccccaaagc
    17281 ccctaacaca gctgcaaata aaacagcttt acgcaaccaa attcccattt tcattatggc
    17341 tcctccttta ttgaaattgg ttcagaataa actgaacagc tttttcagga tctctcgtta
    17401 atccgatata ttctgctcct tttgtcgcaa ccagcttgat tcctaagcga tcggtgtctt
    17461 ctttgatatc atcatagtaa gaattgactt gcggtgctaa gaccaccata tcaaaatcct
    17521 gcagcatttc gtaatgagaa ccataagcac ccgccattgc actgatcggt gctttcgatt
    17581 cagatgcacc tttttttaaa gcattagcta acatcgcact tgtccctgcg ccagcacaaa
    17641 gtaccaacac attgatttct ttatttgcat cgctgataac ttgactcgct gccacaggta
    17701 cttcctcacg tacatcggca gaagcacctt cctcggttgc gatcacctct gctttttgtg
    17761 cttctttctc tactaagaca gcatcgtatg ctttgcaaaa tggtagatag atcaggaagt
    17821 caacgacgat tagaaccgct gctagaacaa aagctaagat acccatgccc gttcctagaa
    17881 ccaaaccgat cggtgctgga gttgcccaag gcaaaacgta cataaagctg ttcatgccca
    17941 atacatcgac aaaaaacttg aacaggcaaa cgtttacaac aggtgttaac aaaaatggaa
    18001 taaaaaaata agggttcaaa atcatgggtg ccgcaaatag caatggttcg ttgacagcga
    18061 aacttactgg aataaaggaa gctttcccaa ctgccttcaa ctgcttagac ttagcaaaga
    18121 gtaggaagat aaaaggaaca accaatgttg ccccagttcc tcccatagtt cctacaaaat
    18181 tccctaaacc caccgtcagt actttagaag cttgttcacc ggaattgaac aattgcagat
    18241 tggtttccac attggcatag ataattgccg caattgccgg ctccacgatc gacggtccat
    18301 gaacaccgac aaaccaaaac agcgccatcg ctccccagat aatagcgatt cctaagtacc
    18361 cgtcagctgc tgtaaatagc ggctgcagca ccgtaatgat tgcttctgca aatgagattt
    18421 ggaagatttg tcgaatgcct aaatcaatta aaacggccgc aaatactgaa aaagagaaag
    18481 gaaaaatatc tcggaaggtt tgcgaaatcg ttccaggaac ttctttaggc attttaatgg
    18541 taatatcttt cagcacacaa aacttatata aattaactgt taaaaatgca gcgacaaagg
    18601 aagccagtag tccctttgtc cccatatatt ccgttgaaaa tccaccttca atcggagaga
    18661 cgcttaacaa caggaaactt accacagagg ccagcatcac agaggtggtg ttgattactt
    18721 tgtttttcgg taagcgacga ttcatcgaat cagccaagca gcgagcagtg gttgctgaaa
    18781 cgagtaaccc tacaacaccc atcgaataat tatagacttt ccatagccat gtggagacag
    18841 attcaggcca ttggtaacca aaaatctcag gaaccgcagc aatcaaaatg aaaatgcttg
    18901 aaaataaaat agctggcatc gctgttaaaa atccgtcacg aacggctcct aaataactat
    18961 tacgtgagat tttttcaaaa aatggtttcc ctttttcgat ttgtttaatg attccatcca
    19021 tagcgttctt ctcctatcct tgattctgtc tgtacagtac aataaagtct tgaatcagat
    19081 ctctcagcaa taaagtggtc attaaatgat cctgcccatg gataaaaatg aatcccatct
    19141 ccatctcttt cccagatgct tcttccgcta aaatttttgt ttgcgaatta tgtgcaaggg
    19201 ttaaattttc gtcggcttct ttcaacgccg aatcaacatg ttcaaagttt ccttgtctta
    19261 cttctttcaa taatttcaat aatgtactgc gagcgtcgcc tgaatatgca acgatctcaa
    19321 aaccaagcat ttgtaattcc tctttattca tcttttcttc ctccattatc acttattaaa
    19381 taatcgtttt ggtttcactg accaacttgt accagtaagc tgattctttt gggtaacgct
    19441 tctgggtttc aaaatcaaca taaaacaagc catacctttt gttataccca tttgtccatg
    19501 agaaaagatc cataagcgac cacaaaaaat accccttcac atttaccccg gctgttatcg
    19561 ccttgcttaa ggattccaaa tagactctca aatagtcgat tctcggctga tccataataa
    19621 tgccatcttc aaactgatct ttatacccca tcccgttctc agtgatatag attttgttgt
    19681 aatgtgggta atcgctttta attcgcagca acaagtcata tagaccttct ggataaatca
    19741 gccagtccca atcagttcga ggaattcctt ctttataaat acgttctccg attcccttca
    19801 ctttataaac agaggtccct ttttcacccg taccgttatg atggattgca ttttctccat
    19861 cgtaagcttt gacaaaatgg cattggtagt gattgatccc taagtagtca ttacgcgtgg
    19921 atgctttttt caattcaacg aaatcttctt caggaaaatg atagctggcc tgatttgctt
    19981 cacatatctc atcaagagca gtcaaggttt cagttgaata atagcctaaa taggtagcat
    20041 ctaataagaa acggatcgac aatgcatcgt ctaaaaaggc agcatgctta tcttccggcg
    20101 cgtctgttgc cgcatatttc gtctctaatg agtgaactac accaatttca cccggaagtt
    20161 cgttttcttt gaaatagttc actacacggg catgagcaac catcatatta tgaagacagg
    20221 cgacgatctt tgtaaaatca tatttgattc ctggtgggaa aacacctaac aaatattgat
    20281 tggttgcaac tggatagatt tcattaaagg tactccacac cttgacttct ttgaattcat
    20341 gaaaacaaaa aatggcatag gaaacaaatg cttctattgt ttctcgattc aaaaaatctc
    20401 catggtcaaa caaacgttta ggggtatcaa aatgatgcaa cgtcacaaac ggcgttacgt
    20461 ttcttttctt acattcttcg aacacacgat gataaaaagc gattccttct ggatttggtt
    20521 tcccagctcc atcaggaaaa atgcggctcc aagcaatgga taatcttaga ccatttacac
    20581 caaaacgttc acacaattca atgtcctttg catattgatg ataaaaatca cttgccggat
    20641 cgggactgaa tcgcccttgt tcctctaaaa agtcatccca agcaacagcg cctttgccac
    20701 cttccttagt ggctccttca acctgatagg ctgctgtggc tccgccaaaa ataaaatctt
    20761 ctggtaattt aagcatgtac atctctcctc aaatctataa tcagttttcg ctctttctaa
    20821 ctgattgcat tgtaaacgca tccaaaacat ttgtcaacaa aaacaaacac aaataatcat
    20881 aaaaaccaga aaacagcaat aaaatctatt gtaacaaaca taaaaaccac cctaaatgtt
    20941 tacttttgtt cgaattagat tgactgttgt ccgtttatat gatagattat ccttaaatta
    21001 gaggtgaaga caatgttgaa aaaagaaaga ttgcttacta tcgtagaaat ggtcaataaa
    21061 aaaggaatcc ttaccgttaa tgaaatcatc aatcaattag atgtttctga tatgaccgtt
    21121 cgcagagatt tagatgaatt agaaaaatcg ggcaagctgc tgcgtgtcca tggaggggct
    21181 caaagtcttt cctacacttt agatcaagaa ctttctcaca ctgaaaaagc cactctgcag
    21241 attcaagaaa aaaaagaaat tgctgccaca gcagcctcct tgattcacga gggagaaacg
    21301 attttcttag gaccaggtac aacaatcgaa ctattggcga cgcatttact gaacaagaaa
    21361 attcggatca ttacaaataa ttatccagtt tttgatattc ttgctcaagc agatggacca
    21421 gaaattctac tgattggcgg tgatttccgg aaaaataccg gcgcatttgt cggaccgatc
    21481 accaacgaca atttaagaag actaaagttt acaaaagcat ttatcagttc aaatggcgtt
    21541 cacaacgaag cgatctctac ttacagtacc gaagaaggag aagcacagca gatcgctcta
    21601 aataattccc gtaagaaata tctactcgtt gataataaaa aattcaatcg agatgatttt
    21661 tatgtgtttt ataatttgca tgatttcgat ctgctcatta cggatcgcca gatctcgcgg
    21721 gaggttcaag agcactatgc acaatatgtc gatctgcaaa ttgctgattc aaccgaaaat
    21781 accactatag gagcgtaact atgaaaggga ttatatttga tttcaacgga acaatgtttt
    21841 tggactctca tctgcatgaa gctgcttggc tgcatatgat acatgatcat acaaaagatg
    21901 gtttatcaga cgaggatatt ttaaaaaata ttcatggtcg gacgaatacg gagattttga
    21961 aacatttcat ttcagagcat ttgaccgcct ctgagatcgc cgtattgtca gaagaaaaag
    22021 aaagctatta tcgtatgctt tgcttgcaaa atgaaaacga gcttcagttg acaaaagggc
    22081 ttgaagcgac tcttgaccag ctgactgacc aacagattcc attaacgatt gccacggcta
    22141 ctgtcaaaga gaatgttgct ttttattttg atattttcga tttagcccgc tggtttgact
    22201 gggacaagat tgtctatgac gacggctctt tccctgggaa accgcaaccg gatatttttc
    22261 ttaaagcggc agacaaactg agcttagctc cggctgattg tcttgttatc gaagatgctt
    22321 actcaggatt acttgctgcc aaacgtgcgg ggatcggaac aattattgca attgatccat
    22381 tcggtaaaaa caaaacagta tttgaacagg cacagctaaa taaagatgga accatcaaag
    22441 attttactgg tttttggaca gagcacctgt ctgccacatc cgttaattct taaacgattc
    22501 aactaactac caaagaaaag caactgaaat gcacacctcc ttttataaaa aactgccagt
    22561 gaaacgacat gaaagataca ctgctatatc cttagtgaaa tatagcagtg tatctttgac
    22621 tttttcattc ttttaaccat atgacgcgtt catctctatc tataattaac attaaaaata
    22681 gtgttttcac attatttctc aaaccaatct taaaaatcat caggagtact tttttgatat
    22741 acttttatta atgctgggcg taatggttaa aagatttcca ctagttcctc tccaccaagc
    22801 tgagttgaaa aatgcttatc tgtaaacaac atagacaaag aggaaataaa cgtttccgta
    22861 tctttctctg tcccaaggat attaattaat tttcctacta ggtaagtatc attaaactta
    22921 agatctgcct tcacatcaaa agttttgata aaagctttta tcatgcgtac aattttgtct
    22981 ttatcgccat aattgatttc accactcata tcaatatagt cactaatccc tgaagaaata
    23041 atggcaactg cttcttcttt cgccttatca gataattcat ttaatgactg ggattgattg
    23101 atgacttctt gagttttatc tgttacgtaa tctctcatta tcctatcggc atactccgca
    23161 aagtaatctt gaaaaaattc ttctacaggg taaccaactt cgttttccca accaggagaa
    23221 atttcattat cagtacttat taaatctatt aaaaaccaac ccgttctctg agtgttgatt
    23281 ctataatatt tttaatttcc tatataaatc aaaaatatta tagaatcaat aagctaatta
    23341 agaaacaaac tttagaaact ctagaagaac ttctgaattt atatagaagt gtgtagctac
    23401 ttactaaata tacaaaagtt aaaataaaat actctggata ctctttatac tgcattaaag
    23461 ttagtaatat taacagcaaa gatatcgaaa aaacaataac ataatatgtg tcaaaatttt
    23521 tcataataag aactccctta ctgttgcata ctaacataat ccaaaaactc ttctacttca
    23581 gctaaagaca ttccactttc tttagtccaa aaaacagttt tttctatcga tccttctctg
    23641 taaactgtaa atgtcggagt cgagttaatt gagtagcgac tcttgaattc tttccactca
    23701 ttttcatctt ctctaacttt tgctacattc acgtaataaa tattttcttt tcccttttct
    23761 accagcatat cgaatagctt cctgcttttg atgatcgatt tcttgtgcta catctagtaa
    23821 attttcatca atttctatat cataaagtat ttcactagtg tctacatcca caatatatac
    23881 tttttatcag tagcaatttt tgagtaatcc gttaaaatga tctcagtctc gtcactttca
    23941 tcagtataaa tatacgttaa caatgaactc accttttcaa tcggtgttag ctctaagcct
    24001 actgtatttt ttactattga gtcattattt accaccataa cttcgtactg tatataccat
    24061 cctatttggt ttccatcaat atcagtaatt ggctcactag ctaatggagt ctccggaaca
    24121 atattttcct gatatttctt aaactcttct gtagcacgaa aaaggttatc atgttcctca
    24181 cttgaaactg gttgagtcat agaaaatgtt acttggctaa acagagggaa aataaacact
    24241 acaatcaaca aagtataaaa aaaagccctt ctttttcatt ttttatcttc caatcacaaa
    24301 ttgaaaaact tattcctgca actttgagtc tagtatcaca atcgactgac tacaactatt
    24361 catttacatt ctactttaag ttcctcctta accattaatt ttattaaaga tacttgtaat
    24421 agtgattctc acaaaatttc atgaaaaata ctttattact gtatctaaaa ttccaataca
    24481 caacttatcg actattatct gaaaaccaaa acttattatt ccttgatgtt attctagcaa
    24541 tagtaactgc ttttttattt ttttgcatat tttttcggcg ttacttttta aagttaactt
    24601 tttcgacaaa tagaaagaga tacgaatcac tgattttttt gaagaaaaga aatggagtga
    24661 gctgaacgtt agagatattg ggagctttcg taactgctta ttaaacgata atggttattt
    24721 gcaaccgtat tgctcgcttc tacatgaaat atttcatgaa atatttcgca aaacctttga
    24781 ttatgtagtt acgtttccat tcttgactaa aaatgtttca aaaacgaaca aaggtgttaa
    24841 aaggtaaagt gatcaatact actggtcaaa ataagaggtt gaaagggaat tgaaaaataa
    24901 ctacagataa tacttttata aacgtacgtg ctttgtggtt ctgtagttaa ctatataact
    24961 ggaattaggg aatggtatca atttgaaaag aaagattcta ccaatttatc atatgttaaa
    25021 aatggaaaat caacagcagt tctttcggaa accttacaca aaaaccgatt attgaaaata
    25081 gccgaaaaac gtttttcggc tattttttta attaagtttc cttcttctta ccttttccca
    25141 ctattccgtt tcacatcttt gatgatcttc agcgcacgtt ttcgtgtttt gatatttggg
    25201 cttttcaagc ggcggtatgc acttgagata tctgtttttt ccatatggac gacccacttt
    25261 cttctttgat tgtagtcatg ctaaaaaaca caaatcgaaa caattacgat ttgtgtttta
    25321 aatggtatca aaagttaaaa tgaaagtcaa cttgttaaaa cagtcgatca gactagctga
    25381 ttgactccta tctctggaaa taaattttca ttattacgtt ttagctacct aaaaaaggcc
    25441 ctcttgtccg ttaactttaa tatctattgt tatagctagt ttacctattg aacgtactgt
    25501 gtgttcgata gaacactaaa aaagaggctg ggacagaagc gtttaactcc aagaa
//
LOCUS       DACECR010000037        25315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.037, whole genome shotgun sequence.
ACCESSION   DACECR010000037 DACECR010000000
VERSION     DACECR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.037"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..276)
                     /locus_tag="IWO17_001367"
     CDS             complement(<1..276)
                     /locus_tag="IWO17_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290251.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAI"
     gene            complement(861..1568)
                     /locus_tag="IWO17_001368"
     CDS             complement(861..1568)
                     /locus_tag="IWO17_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290252.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1633..2469)
                     /locus_tag="IWO17_001369"
     CDS             complement(1633..2469)
                     /locus_tag="IWO17_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0290253.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2487..3896)
                     /locus_tag="IWO17_001370"
     CDS             complement(2487..3896)
                     /locus_tag="IWO17_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0290254.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3910..5733)
                     /locus_tag="IWO17_001371"
     CDS             complement(3910..5733)
                     /locus_tag="IWO17_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0290255.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6576..6902)
                     /locus_tag="IWO17_001372"
     CDS             complement(6576..6902)
                     /locus_tag="IWO17_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290256.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6948..7310)
                     /locus_tag="IWO17_001373"
     CDS             complement(6948..7310)
                     /locus_tag="IWO17_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ0290257.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7347..7562)
                     /locus_tag="IWO17_001374"
     CDS             complement(7347..7562)
                     /locus_tag="IWO17_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0290258.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7689..8024
                     /locus_tag="IWO17_001375"
     CDS             7689..8024
                     /locus_tag="IWO17_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0290259.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(8100..10097)
                     /locus_tag="IWO17_001376"
     CDS             complement(8100..10097)
                     /locus_tag="IWO17_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290260.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(10195..10470)
                     /locus_tag="IWO17_001377"
     CDS             complement(10195..10470)
                     /locus_tag="IWO17_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0290261.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10702..11436
                     /locus_tag="IWO17_001378"
     CDS             10702..11436
                     /locus_tag="IWO17_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0290262.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11475..12617
                     /locus_tag="IWO17_001379"
     CDS             11475..12617
                     /locus_tag="IWO17_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0290263.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12712..14103)
                     /gene="sufB"
                     /locus_tag="IWO17_001380"
     CDS             complement(12712..14103)
                     /gene="sufB"
                     /locus_tag="IWO17_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ0290264.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14108..14578)
                     /locus_tag="IWO17_001381"
     CDS             complement(14108..14578)
                     /locus_tag="IWO17_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ0290265.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14565..15800)
                     /locus_tag="IWO17_001382"
     CDS             complement(14565..15800)
                     /locus_tag="IWO17_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0290266.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15797..17083)
                     /gene="sufD"
                     /locus_tag="IWO17_001383"
     CDS             complement(15797..17083)
                     /gene="sufD"
                     /locus_tag="IWO17_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ0290267.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(17096..17869)
                     /gene="sufC"
                     /locus_tag="IWO17_001384"
     CDS             complement(17096..17869)
                     /gene="sufC"
                     /locus_tag="IWO17_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ0290268.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(18066..18914)
                     /locus_tag="IWO17_001385"
     CDS             complement(18066..18914)
                     /locus_tag="IWO17_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0290269.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18938..19624)
                     /locus_tag="IWO17_001386"
     CDS             complement(18938..19624)
                     /locus_tag="IWO17_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0290270.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19617..20651)
                     /locus_tag="IWO17_001387"
     CDS             complement(19617..20651)
                     /locus_tag="IWO17_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0290271.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(21046..21384)
                     /locus_tag="IWO17_001388"
     CDS             complement(21046..21384)
                     /locus_tag="IWO17_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ0290272.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21386..21742)
                     /locus_tag="IWO17_001389"
     CDS             complement(21386..21742)
                     /locus_tag="IWO17_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ0290273.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21748..22935)
                     /locus_tag="IWO17_001390"
     CDS             complement(21748..22935)
                     /locus_tag="IWO17_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0290274.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(23126..23971)
                     /locus_tag="IWO17_001391"
     CDS             complement(23126..23971)
                     /locus_tag="IWO17_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0290275.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            24205..25170
                     /locus_tag="IWO17_001392"
     CDS             24205..25170
                     /locus_tag="IWO17_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ0290276.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7418 a         5313 c         4279 g         8305 t
ORIGIN      
        1 aattgcatat cccaaaatat caacgattgt ggcaatcaga tcttcttttg attcttcatc
       61 tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga
      121 ttcatctgct aaaagaacca cttgttccaa caatcgatcg gataaatatt tatagaaaat
      181 cagtcccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
      241 cgcactaaat aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt
      301 taaagttgct atataccctc ttattttagc ataccccctt ttataaaaag caaaaaaaaa
      361 aagaaagaaa cttattcttg ctaataaact acgcgaaagg agtctaacgt tgtttgatat
      421 aacttagact gatcgttaac ttatttttta tatttctaca tagttatctt tagaaccatt
      481 tcagatacat atgaaacaat tacatatgat tttttcataa aaaaacagta ctgtctagta
      541 gtataaaata tacaaatttc ttaaaaattt tttcactaaa atcactacct gaaacagtct
      601 aaaacactta tatatcaacg tttaatgaaa ctatctccgt ttcatttcag atacaaataa
      661 atatagtcgg gctccaaatc cgaaaaaaaa tacccccagt agcttatcct aagctactgg
      721 agatgtataa gtacggtcat ataacaacgg ggtagggtaa ataaatgaaa aagatggaag
      781 aatcattatt caataacttt tttaaatact tcgttgtaat cagttacctg tccgttctta
      841 aataccacag aataatcttt ttacccattt tcaagtaacc gaagtggaat gtctttacca
      901 acttgctctg tcactgcatt actgatacct gttagattag ctaataaatc tgataaagat
      961 tgagcatctg tagaaatctc tgtatcagta tttatcgtga ttcctttatc ttcatttaca
     1021 agcttccaag taggtggtat agctgtgtat gtttcgatga atgaatcaat tctttcatct
     1081 atcgtcttcc cagtatctac ttctgtctca ttactcttag atgaatcgat tctttcatcc
     1141 atcgtctgct ccgtaactgc ttctgtctca ttattctcac cggaaactct cttcacaccc
     1201 aaacttatat attgtccatc tttagtgtgc atagttaaaa ttcccacgct tttactaata
     1261 aattcactat aaccagttct ttcaatagag acttcaatgt atttcacact atcatactca
     1321 aaagaaaaat agacatgtaa aagcattttg ttgtttacat ctacataata atcctttgac
     1381 tctacttgag accttcctat acggaataca gctttactgt ctgatatctc tataccagca
     1441 tccttattat taatctgcca atctccaagt agctcttctg gcaagctagt agtttcttgt
     1501 gaacacttta ccacttcact ggtagaggtc gaaacattca cctccgcact gttagaagaa
     1561 ttttccaagg aatcaggagc cttagcacaa ccaacaagtc tttatatatt gtgcagccga
     1621 tattttcttc attcagtaca cccttgaaat atgcagtgtt agataaagca gttcgtcctg
     1681 atttgctgag gcacccatct cttcttgaac gtactccttg attttcaagg ccgcccgata
     1741 ctctttcgga aaacgctgct tcactgaagc cagcaaaaaa tcatccttct ccggcaactc
     1801 ttccttctta atcattcggg caataaaaaa ctgtaaatgc gtcacaaatc ttaagtaaag
     1861 catcgaatct ttatcaacct ttttttcggg aaacagatat cctagcatag ccaagatatc
     1921 ctttagtgct cttaccgaga gcatggtttc ttccatattt gcttgttcac tttgggcatt
     1981 tacaatgtga aacgcaatgt ttccagcctc ctcttctggc aactgaacat tcattttttt
     2041 gttgatcaat tggatagcat agcagccgat ctcaaattct tttgggtaat acttttgaat
     2101 ttcaatcagc aggcgatttt gaaaaacaat gcctttttga ctgcgttcaa tggcatgctt
     2161 caagtgatca accaaggtaa aaaatagact tttattcagc ttaaagtcaa acttggcgtt
     2221 ggcataatcg ataattcgct tgacgcaaat aattagatca tccggagcag cctcaaacag
     2281 tttaatgtaa tcctttttta gagcatcaga aagtgaatct aatttaaata ctttttggat
     2341 tttgtcagcc ggaaccaaat cgcctttctt tagttgaaat ccaatccctt tccccatcaa
     2401 aattacttct ttgccttgct tatctaagca aagaattaaa ctgttgttta acgttttaat
     2461 tactctcatt agattcaccg ttctctctaa gctaagccat ttcttgcgat tactttttga
     2521 taccaaaaga agctttcttt ttttattcgt tttaattctt taagattctc atcggttcgg
     2581 tcaacatata taaaaccata acgcttattg aagccttgat gggtactaac aacatcaatc
     2641 gctgaccaag gtaaataacc aaaaacatct acaccatctg ttaaagctgc ttgaatagcc
     2701 agaatatgtt tggctagata atcaattcga tcctggtcat taattcgtct atcttcctca
     2761 actatgtcgg gtgccccata gccattttca gtaattaaaa taggtaaatc atagcgatcg
     2821 tacaatttcc tcaatgttaa tcgtagtcca gtagggtcaa ctacccagcc atatggcgtt
     2881 ttatctacat aaggattttc agctgcccgg tagacacctt cttctcccaa catgatctgt
     2941 tgatcccccg ctctagcgga aacatctgat ccgtcatttt tgctggcagc gatggtggca
     3001 gttgaataat aattaatcgc aataaaatcg ggtttaccgg aggataaaat tgcctgatcc
     3061 tctgcctcaa ttttaggata taatccacga tcattcaaat agttttcaaa caaataattg
     3121 tattttcccc gaactgctac atctaagaaa gaccagcctc tcaatgtttc ccaattatgg
     3181 gctgcaattg catcttctgg actggaggta gcttgataca tagccgttaa attgattgct
     3241 ggaccgattt ttcctttaag attcaactga tgatacaagt gaattactct agcttgagct
     3301 aataaaacat ggtgattaat ttgaaagagt tctttttttg aaggcagctc tccacttggt
     3361 ggcaaaccaa tcgctccagg atgaaggatc atcgtgtttt gctcattaat tgtcagccaa
     3421 tagttcacct tgtcaccata ctcagtaagc aatacctgta catagtccac aaaataatcg
     3481 atagttgctc gatttaacca accgccctgc tcctctagaa aataaggtaa atcgaaatgg
     3541 tacatggtga caatcggttc gattccctga ttcaggcatt catcaattac ttgatgataa
     3601 aaagaaattc ccttttgatt ggttttatta ttttcaatat ccataattcg cgtccaagcg
     3661 atggaaaagc gaaaagcttt taagcctaat tctttaaaca gctgtatatc ttctttatag
     3721 tgatggtaaa aatccgaggc aacttcaaaa ttagctaccc cttctgtatg ctgatacttg
     3781 tcaataatag acggttgttt cccatcctcc tgataggcac cctctgcctg gtaggcacta
     3841 atggaggcac cccataaaaa cttctctgga aatgctgaaa gctgtttgta ttgcacaaaa
     3901 atcctctcct taactaattt gcatcaatgg ttcgtgttcc tttaagcttc cgcttgcttc
     3961 actaattcct gcgtaatttt cactgttggt cactaccaca atcactgtat catcgtagcc
     4021 agcgtttcta atggcagcga aatcggcgtt gattaataat tgtcctgctg acacctgatc
     4081 tcctgctttt acaaatactt caaagggttc cccttttaaa ttcacggtgt cgatccccac
     4141 atggatcaat aattcaatac cttctttaga acgtagacca atcgcatgtt tagattctaa
     4201 aatagtaatg acttctccct caacaggtga ataaatgctg ttttcttctg gatgaatggc
     4261 tacgcccttt cccaatagtc ccttagaaaa gacagcatcg ttcacttcag aaataggaca
     4321 cacggttcct tttaccggat tttcaacaat gactgcttcg gctattgccg tttgctctga
     4381 ttcatcagaa gtatcgtcaa atccaattag ataagcaatg ataaaggttg caacaaaggc
     4441 gattacacac gtaataattg cgtgaacaat attcattgga ttttctccaa taaaggctgg
     4501 taaggctgct aagccagggg aaacaaaggc atatctgact aaaccgctca agccagcata
     4561 caaaccagca attgcaccac caatcatgga agcaactaat ggttttttca gcttcaaggt
     4621 tactccatag agtgaaggct cagtaattcc cattaaagca gtgatcccag tggacacagc
     4681 tagctgcttc aactctttgt ttttagtttt cgcggctaca gcaaaggtag cacctgattg
     4741 agcaatatta gaagccagca ttcctggccc attgatcatt tcagaacccg tactgctcaa
     4801 ttgcatagta gcgattggcg tcattgccca agctgtaccc gtaaccacca aaaatggctg
     4861 caaaccaccc atcaaaaatg gaattaacca agctgctttg ccgttaatcc attcagcccc
     4921 cattgctata agatcgttca gcaacgttcc aaacggtcca atgacaacca acgctaaagg
     4981 ccccataatt aaaaatacca gcatcggctt agtaaagaat ttaataaacg aaggcgaaat
     5041 tcgatccgca aagggctcta cataggacat gatccaaaca gtaaagataa tcggtaaaac
     5101 tgatcccgca taatctgcag ctaataccgg aattcctaga aaagaaacgg catcgccagt
     5161 agaaaaaagc tgtgaaatat ttggatgaag caaaatgcca gcaacagtaa tcgccaaaat
     5221 tgggttgaca tcaaacttta tggctgcccc ataggctaat agtactggca taaaataaaa
     5281 ggctgcatca gcaatagtat tcaaaatgat gtaagtttga ctactgtctg atagtacacc
     5341 agtcaaagtc aagacggcca aaatggcttt taccatcccg gcacctgtta tagctggaat
     5401 catcggtgta aaggtggtag aaatgacact aatcaatctt gaaataatac tttcttgatt
     5461 gtctgaagag gggccctttt taattgaagt tcccacttcc ttttgaattt ccttgaaggc
     5521 tgtttggact tcattgccaa tcaccacttg gtattgtcca ccgctatcca ccacactaat
     5581 tacaccaagt gttttttcta aggctttcgt atcggctttc cttttatctt tgagttcaaa
     5641 acgcagtctg gtggcacaat gggttaagct gaccacattt tcttttcccc caacctgttg
     5701 caatacattt cttcctactt ctctgtaatc cattattctc tctccttaaa gcagtaaaaa
     5761 agaccaagtc aaaccacgcc ccaatcaaca ttgaagtgtt agtttgtctt ggtcttgcct
     5821 gcataatcag taacatgcca ataacgttat tttgctgtaa tccaataata gcacattatg
     5881 atagcgcaat caacagggaa tttaattttt tatcttttcg tttgaccgta tagggtaata
     5941 taacagttag atcacatcaa ctagggtata tctgcgtata cttactgcta aaataagtat
     6001 atctaatatt aactatacca gtaaaaccat tcctacataa ccagcgggaa attcccaaaa
     6061 tcgtgaaagc atcaaaaatg catataacgg atcacacaac ttcaacaatt ctgctcttgg
     6121 gaaaaattga tgaatttggt gagtaaagga tggaataaac agcattccaa caatataggc
     6181 aacaatagtg atacttgttt ttttggtaaa aatcagtaag caatagccaa tttcaatcat
     6241 tccgagatat aaaggtatcg atagaatgat ttgggctagt attcttaaaa attaactagc
     6301 aaatgtttaa actgatttat ttctaatcta catgtagtaa gtatttcaat tccaccaatt
     6361 atcaataaaa ataccaaaca tatccataat aattttttct ttttcatttc ttttatattg
     6421 ttataacagt gaataacata aaaattcccg acaatcattg taatattttt ttgtcacctg
     6481 tattagtatt taatttatag gaaactataa aaactcccta gtgaatttac acctttggga
     6541 gttcctgttt ggacggtatg cataatacat ttaaatcaat ttaacattaa cacctcagca
     6601 tatggtatct ccacatatat acggtctttc tttggatagt actcaatatt tatgttgata
     6661 gcacttccat tgtctaatat agctttagcc cggtatgtgt tatctccatt ttcatgagta
     6721 atcgtcacat catcacatga tcgatttaag ttgtactgtt cttgtaaaat ctgtgttacc
     6781 aatggttttg atgacttctc taatccagat gtacctgtca tttttgaaaa aataatacct
     6841 ataatagcca agacaacaat acctataatg acaaacttct tgttcatatt tactttattc
     6901 aactttttat ctcctatctc caaaataatt tgttcttatc cctcttatca gtacattgac
     6961 atccctaaaa gtacaccaaa gattgctcca gctacccctg ctatcatcaa aatcgtaccc
     7021 attttctcat ctttttctcg atataagtag cccataataa cagcaacagc accaaataca
     7081 acaggaataa atgctaacga tactaccata gaaatccaac caataatcaa atacagcttc
     7141 cctgtctctt gtccatgtac gatttgttcc ttcatttcat taattgtctg ctcagtactt
     7201 ttctttactg tgtctgttcc ggttgtctca tctgtttctt gtagtgctcc acaaaactgg
     7261 caatacttac tttcatctgt tatttctttg ccacattttc tacaatacat gtcttctcct
     7321 cctaaaatca ttcaattgct tattagttaa ctgtttactt tattaaatac atcagaaaac
     7381 agctcattca ttggtacact taatatctca gaaagcatct tcatttgata gggtttagga
     7441 atcgttctcc ctgtttgcca cgaacttaca cattgctgtg taacattcat tttttttgct
     7501 aactccgttt gagaaagtcc tttttctttt ctgtatcggc tgagattact tacttttttc
     7561 atgattctcc cctcctgttt tattatatac aacaatatga tgtatttcaa ctcataaaat
     7621 ataaaaaact tcaaatacca ttacaaatac aacaacttgt tgtactatac tgagatgggg
     7681 gtgtattaat gaaacagaaa caatatgcaa aggtcattag agaaaagcga aaagctctcg
     7741 ggtggacaca aaaagaatta gctgaaaaaa tcttctctac acaacaggca gtagcaagat
     7801 gggaaaattc tgtaacagag cctaacctcg atagcttaac agctctttct agagccctag
     7861 gaacccctgt aagccatttt ctagacaatg ttgtcgtaga ttatgaggaa gagtttttgg
     7921 ctctctatcg ttctcttagc actgaagatg cagttcgaac aattgactat atgaagctat
     7981 taaaaaggca agaaaatgaa cgcaatcagc tattgaaaga ttgaaaaata aataacccac
     8041 cgaactttaa cggtgggtgt attgagatga aataagatta agagctatgg ataacctgtc
     8101 tagtttaata caattgggtc ttttctcttc cttactctcc gtccatcatc tcttgttaca
     8161 cttgtaaatt gatacagagg aactatccca gctcctgata atatcaattc ttttattgtt
     8221 tcatctatcc ctgccccttt cgctaaatat attaaaaaac tactacttgc tttcacattg
     8281 tatagtttct taatatcctt agaagctcgt tcaagagtga atccttgttt aacaaagtag
     8341 cctctgtttt ttattaaagc atttttcacg ctgttatccc caacattacg taggcgttca
     8401 caaacagtgt taataagttc tttctctgtc attagatatg tcccttgcca cgataagccc
     8461 tgtaaaactt catgtgcttc atctaaaatc aattgttttt ccttgctgag ttctgatcgg
     8521 gaattttgag gaaatactct tttagcctct ttttcgttaa aacatatctt aacccattca
     8581 taagaaaaat attgatatgt aaaatgaatt ttttccagaa gtggaaggat atcttcattc
     8641 aatttttgaa tcacagaagt tgcattaaaa acgtctaatc gatagacatt gcttctctta
     8701 tgattaaagt ttcctaacgc tttctcttcc tttaacctat ttaggtattc gagcatcttc
     8761 aaaggaacat cttcatcatc taattttttt atcaatccta taaaagtcat aagtgatagt
     8821 aacttattac atttatctat gtcataagtt ttaccatagt aagaactcat ttttttagct
     8881 aattgttccc ccgacaaaat acaagtcaca ctttccttac tatcatcaaa aaaattaaca
     8941 tcagaaagca agacatcata gacacgtcgc atttcctcta gataattcct tattgtcctg
     9001 aaaagtttgt ctttcattga ttcaagagtg tcaaaaagta cttttgattc ctctttcaat
     9061 tttcgaaaat gtgtaggata caagtctagg ttctctaata gccaattaaa tgtttctacc
     9121 cgactgtctt tctttaaaat tagcatcaca actccaataa aattttcact gatatcacaa
     9181 ttactggaat gacaataaaa aatggaatgc ccagttttac tggtaaaaat actagcagag
     9241 ggattatgat cgttgtgaaa cagacatctt aagtttttac ttgacttaag taatttattc
     9301 atatcaatgg ttaacaatct ttcataaatc tcattaaaag acatgtcgga ggaatcaaac
     9361 aggcagtctt taaaccactg tttcatttct tctttgtcgt cattttttat caacttaaca
     9421 aaatcggttt cattgactag ttcatactct ctccctgaat cactttgaca atttttagaa
     9481 catgtcatgg taaaatcaat cggaagctca gtaatatcta acatgttgtc ttcattaatt
     9541 attatcactg catctgactt atatcctcca aaaaacattc tattcgaagc atttacattg
     9601 tcatcacacc cggggatgtt ctttttcaag tattcgtaaa cagcaaccat tttaccatag
     9661 tcagtaattg cttcttttaa tagaaagaca atcttgtatt tttctagatt ttcagagtac
     9721 cgaatacttt tttgtatcgc aaacgcagaa ttatgaacta aattgataga taaaaccttt
     9781 tctatgctta agtatctact atcatcttta ctcaaatcta ctaccttccc cttctcatct
     9841 ttaacagtgt tatcgacatc cagaataatt actctggttt ccacaaaact ctccttgtca
     9901 ttgcctttat tctttgtctt gcttggaata aatgattgtg atgaatttag tatctctaca
     9961 aactcatctc ttgtaacttc tctatagtta tctaaacacc tatctcttac atcagttttt
    10021 tctttttttg taactggctt ctctaataat ttttcttgat ctacataaat tggaatatta
    10081 atcattgatg tttccatttg tgtttcctcc ttataaaatt ctgttaaaga ctaaaacata
    10141 cagataataa aaacaaactc tttattacta tatgttttag tcttatttgt ttactcaacc
    10201 tttataccgt tccatgaaac tatcaatatc agatttttta aatctaatga ccgtatcaat
    10261 ttttataaca ggtaacccgt attgagaaac atattttcta atcgttccta ctgatacacc
    10321 tagatattca cttgcctgct gatagtccat atactctaga gtgactccag aaataatgtc
    10381 ttctagcata ggctttagca ctgtaattat ctctttcact tgttgttcaa cttgtataga
    10441 aacaatgcta tcaatgattt gtagagacat atttccccct cctttcgtga ttgcttgaac
    10501 aaattttcta agaattatct tcgtattttt tgctcaaaat aaaagtattt tgttattaac
    10561 cgattaatca aacaaaaaat gattaccagt tcatcgacat acacataata cctcgataaa
    10621 aaaggggtgt caacagcttt gtaaaattta tttacaacac cccttttttg aggtatacta
    10681 attttaggag gtgtggtcaa tatgataaaa cgtccaccaa taaattattt agaacgaaaa
    10741 aagatactcg gaacaaaaat aaaagctata agaaagagta aaaaattaac acaaccagca
    10801 tttggtctta tgattaacaa cggtcaatta attgacaaaa aaactatcta cgaatgggag
    10861 aagggaacct atttaccaat accagagcga ttatcccgaa ttgctgattt gggaaatatg
    10921 agtatagagg agttagtttg tggcaatgta gaagaatata ttttgggaat tattctttat
    10981 agagatagca ttgtactaga tggtataaca tttccagata aaaacctgtt ccagcatctt
    11041 cgtcaacagt tcccaccagt acactctaat ttagatacat ggctggatag atattccaaa
    11101 ttagaaccag agatgcaaga atttattgct aacaagactt gtaataaagt taaaaatgaa
    11161 aaaattagtc ttttcaatat cttaaaaata gaagaattat tcatcaatgc tattgtagaa
    11221 gaatttgaca ataacattct tttcctcact agcagtatag aagaactact agaaagaatg
    11281 gtagatgaat ggctacctat tcaattgaaa gacatgtctt atcccgaaga agctgtgaga
    11341 gaaattactg ataatataaa caagctagaa caaacgattt catcaattgg gaaaaaatac
    11401 actaagaaaa aaatgaaggg aggtgatacc atctagtggt aagtactcct tgtaaactaa
    11461 gaggagatga tttaatggca aaaatatcaa agtacaaaaa gaaaaacggt caagtggcgt
    11521 ggatgtataa aggacacatt gccaccgatc cacgaactgg tgaaaaaata aatactacga
    11581 gaagaggttt tagtagcaaa caagaagctc aaaaagatta tgacgagtac agacatcaaa
    11641 tattatacgg tgttaaaaag aaagcacctg atatgacatt tgaagacctg tataatgaat
    11701 ggataactca tcagcgtacc agtgttaaag cttcaacaat cgcaatctca gtaagatacg
    11761 caaataatca aatacttcca gcatttggta agcttaaact atcgaatatc tctgtcccat
    11821 attgtcaaaa agtggtagat gaatggcaca gtaaatatga aagttacgat tatatgagaa
    11881 aacagactgc acaaatttta cgctacgggg ttgcaatgca atatattgac aataatccta
    11941 tggaaaaaac acttcttcca cgaaaaaaag agtatgagaa aaatcgtaaa ttttactcaa
    12001 aagaggagct taataattta ctggatgctt ttaaagattt tggtaacatg aaacaatacg
    12061 ccttcttcag attattggca tacacaggta tgagaaagag tgaagtactc gctttacagt
    12121 ggaaagacat agacacgttt aataaagaac tacatgtcaa taaaactctt gctgtagatg
    12181 aatttggtaa agttataatt caatctccga aaacaagagc ttctagacga gttatctctt
    12241 tagatactga gactctttct atcctcaaca actggaaatt acagcaaaaa gaagagtatt
    12301 taaaacttgg atacaatact agctctaaag agcaacatgt ttttacaaca gttaaaaaca
    12361 ctctatacat tccaaacact gttaatgatt ggttaagata tattttgaaa aaatataact
    12421 tgcctcgtat tacacctcat gggtttagac atacacatgc tagtttatta ttagaagcag
    12481 gagaatctgt caaggtagtt caacaacgat tagggcatga aaatagtaaa gtgacattag
    12541 acatctatgc acatattaca aataatgcac ctaagaaaac aggtcaagac tttgctgata
    12601 tgatggctca tcagtaaaag agctggtcaa aatctggtca tttctagtat acaggcaaaa
    12661 caaaaaagct agaaccctta tatatcaagg gttctagctc ctataacagg tttacccaac
    12721 actgccttcc atctcataac tgatcaatcg attgagttcg actgcatatt ccattggaag
    12781 ttctttggtg aatggttcaa caaagcccat gacgatcatt tcagttgctt cagattcggt
    12841 caggcctcgg ctcatcagat agtagagttg ttcttctgag atttttgaga ctttggcttc
    12901 gtgttctaaa gagacttggc tgttgtggat ctcattgaat gggatcgtat cggatttcga
    12961 ccattcgtcc atgatgatcg tgtcacattc gatatgagag atcgaacccg cactgttttt
    13021 ggcaaaggtt acttgtcctc gatagtttac ttctccgccg tcttttgaaa ttgatttaga
    13081 gacgatagag ctagatgtat ttggtgcatt gtggatcatt tttgcacccg tatcttggat
    13141 ttggtttgct cctgcaaagg cgatggagag catggtgcct cgtgcacctt ttccatctaa
    13201 gtaaacactt gggtatttca tcgttgtttt tgcacctagg tttccgtcga tccattcaac
    13261 ggttgcgcct tcgtatgctt ttgctcgttt ggttactaag ttgtacacat tgtctgacca
    13321 gttttggatc gttgtgtaac ggcaatacgc atcttttctt gtaaagattt ctacgatggc
    13381 ggcatgcagg ctgttacttg aataagtagg tgccgtacaa ccttcgacat agtggatgct
    13441 tgctccttcg tcaacaatga tcagcgtacg ttcgaattgc cccgtgtttt ccgcattgat
    13501 ccggaagtac gtttgtaatg gaacatcgac tttgacggct tttggcacgt aaataaaggt
    13561 cccacctgac catacggctg aattcagcgc tgctaatttg ttgtcggttg gcggtacaag
    13621 ttttgaaaag tactctttga ataattcagg gtattctttc aaagcagagt cagtgtcagt
    13681 aaagacgatt cctaactttt cgaattcatc tttcatatta tggtacacga cttctgattc
    13741 atattgtgcg gaagcaccgg ctagataagc tcgttcagct tctggtatac caatcttttc
    13801 aaatgtatct ttgatttttt ctggtacgtc ttcccagtca cgagcgggtt tgtcacttgg
    13861 tttttggtaa tatttgatgg catcgaaatc gatgtctgaa aggtcggggc cccatgtttg
    13921 cattggcatt ttgttaaatg tttctagaga tttcagacgg aattcaagca tccattctgg
    13981 ttcgcctttg attcttgaca tctcacggat cacttcttct gtcaagcctt ctcctgtact
    14041 gaaaaccggt tcgacatcat cgtgaaaccc gaatttatat tcttctaatt ctggtacgcc
    14101 catgttctca ctcctcttcc ttgtggtaat gatggaaagc tgcggtacca tcgttttcca
    14161 ttgcttttcc tagtgctttc caggcaagtg tcgcacattt gatccgagcg ggaaatttag
    14221 ctacaccacc aagcatagca gcatcgccta attggtcggg atctttgact tcttttcctt
    14281 ggacaagctg tagaaagtct acagttaaat gttctgcttc ttctattgat tttccgatga
    14341 cggcgtctgt catcatcgag gcacttgccg tactgatgga gcagccactg ccgtcaaagg
    14401 cgatattttt gatgattccg tcttttacat cgacttgcaa ttcgatcaca tctccacatg
    14461 tggggttgtt caattcgatc ttgcttgtcg agtcagtcaa gcttccacga tgatgcggat
    14521 gggaagaatg atccaagatc acttgacggt ataaattgtc taatttagat agtcccatgt
    14581 tggaaaaact cctttgttgc catgattgct tcaactaaac ggtcggcgtc ttgcgttgtg
    14641 ttgtagaaat aaaagctcgc gcgagcagtc gctgatactt caaggtactt caatagcggt
    14701 tgcgcacaat gatgacctgc tctgacggct accccttcca tgtctaaagc tgtcgcgaca
    14761 tcatggggat gtaacccatc caaattgaat gcaacgactc ctgtccgaaa attcgggtct
    14821 tgtggaccat aaacagtcaa tccatcaatt gcttgtaatt ttggcaacac ataagcggct
    14881 agttcttctt catatcgatg gatattgtcc ataccgagtt tttgcaaata atcgattgca
    14941 tggcctaatg cgatagcgcc ggcgatattt ggtgtccctg cttcgaattt ccaaggtagt
    15001 tctttccatg tactgtcgta taattcgaca aaatcgatca tttcgccacc aaattcaaca
    15061 ggttccattt cttctaacca ttttcgttta ccatataaaa caccaatacc tgttggtccg
    15121 cacattttgt gtccgctgaa agcaaagaaa tcacagtcta gtgcttggac atcaatggcc
    15181 atgtgcgggg cagattgagc accatctact acgatgaccc cgccgttttc atggacaagt
    15241 tcagctaatt cttgaacagg attgaagaca cctaagacat tggatacatg agcaatggag
    15301 acgattttgg ttttgtccgt aatctttttc gctgcatcct ccatatccag gaagccttct
    15361 tctgttagtt cgatatattt caagacagca cctgttcgtt ttgccaactg ctgccacggg
    15421 ataacattag aatgatgttc catgtaagag agcactatct cgtctcctgc tttgatatag
    15481 cggtcgccga aggttctggc tacccaattc aagcttgtcg tcgtaccacg agtaaacagc
    15541 acttctgctg tttcttttgc atggatgaac gcccgtactt tttctcttgc ttgttcataa
    15601 tcatgggtcg cgcgttctgc taacgtatgg acaccgcgat gaacattggc gttatcgtgt
    15661 tcgtaatagt acgctaatgc ctggagtact tgttttggct tttgggtcgt cgctgcatta
    15721 tccagataaa ctaaaggttc atcattgacg tcttgaaata agataggaaa atcattgcgt
    15781 agctttttag catcgatcat ccattcaact tcccttcgat tacttcgact aattctttcc
    15841 gaacttgttc tacagggatt gctgttaaga cagaaccgag gaatcctcga atcactaagc
    15901 gctctgcttc ttctttatgc aatcctctac tcatcagata gtacatttct tctggatcaa
    15961 ctcgtcctac actggcagca tgtccagcag ttacttcgtt ttcatcgatc aaaagaatag
    16021 gattcgcatc accacgtgct ttatcagaaa gcatcaagac acgactttct tgttgggcat
    16081 ctgcaccttt tgcccctttc aagatgtggc cgataccatt aaaggtcaat gttccttttt
    16141 ctcggatcac tccgtgttgc aaaatatgcc cgatcgtatg aggggcttta ttggttactc
    16201 gtgtatcgat tccttggatt tgtctgcccg agctgattgc cacgattttg acttctgcgt
    16261 gtgcaccttc tcctgctaaa tctgagtcaa agtcagcgat cacatgaccg tcattcatga
    16321 caccaattgc ccagtcgaca gaagcatctc gtaaaatgtg cccacggcga ttcatataag
    16381 aagtaatatt ttctcctaat tgatctacgg ctgaatattt gatttttgaa ccagctttag
    16441 cgatgacttc cacgatgata ttacctgatg attttgctac ttgttctttg gttgtctgga
    16501 aacgttccag ataagaaaat tcactatgtt catctgccac gatcaacaca tgcttgaaga
    16561 agtgctcatc ggatgcgcca tcttgaatga ataaggactc gagtggttca tcgatcacga
    16621 catttttagg cacatacagg aaaataccgc tgttcataaa agctacatgg agtgcagtca
    16681 attggtcttc gttcatgttt actgcttttt tcatataata ttcttggact agttctggat
    16741 attcgatcat tgcagtgaac aagtcggtaa aaatgactcc tttttctgct aattccacag
    16801 gcagttgttc aaaaatcgtc catgaacctt gttggacaag cacaggattg tctttcattg
    16861 catcaaatgc cggaatcgtg cctgtttcac tgatggtttg tgtttctttc acatcaaata
    16921 acgaccagcg atggaatttc acccgatcga tatgcggcaa tggcagttca tctgctttcg
    16981 ctagtgcgtc ttgacggaag gttctcatcc aatcaggttc gcctttactt aaagaaaaat
    17041 catttaccgc gtcaagataa tcgagccagt ttttttcttt cattcttgct cccccttacg
    17101 cttcttcttc tttatagtcg attcctaatt ctttactgat accagcgtag ccttctgctt
    17161 ccagacgttt agctaggtca gcattccctg tcatgactac tcggccttcc atcatgatgt
    17221 gaacgacatc aggtgtgata tagttcaata atcgttgata gtgggtgatg atcaatgcac
    17281 cgaaattgtc tccgcgcatt tcatttactc ctttagcaac gactttcaaa gcatcgatat
    17341 ccagacctga atcgatttcg tctaaaattg caaatgtagg ttctaacatc aacaattgca
    17401 ggatctcatt ccgtttcttt tctccgccag aaaaaccttc gttcagataa cgttcagcca
    17461 tttcttctgg catatttagt aaagccattt tttcatctaa ctttttcaaa aatgccataa
    17521 cagaaatttt gtcgttttca tcacgtttcg cattgatcgc tgcacgcatg aattccgcat
    17581 tcgtgatacc tggtatctca cttggatatt gcatagctaa aaataagcca agtcgcgcac
    17641 gttcatctac ttccaagtct aaaacatttt tcccgtcaaa caaaatttcg ccttctgtta
    17701 cttcatagtt tggattgccc atgatagcgg cagataaggt agattttcct gtgccgttag
    17761 gtcccatgat ggcatggatt tctccggttt tcattgttag gtttacgcct ttaaggattt
    17821 ctttttcctc gatcgacaca tgtagatttt ttatttctaa gacagacatg cattataccc
    17881 ccaaattctc tttcgttctc actcatttta gactaattga gaatgaagcg caatgacctt
    17941 ccttactaaa atcaaatatc ttttatttat tattaaacaa ttttgatttc taaattagaa
    18001 taggtataaa aaagatgaga aataagccat tacagactta tttctcatct tttttgttcc
    18061 ataccttatt ttgcatcagc ctttactggt ttaacagagc cgccccattt gtctgtgatc
    18121 cattcttgga cttctttgcg gtgaagcact tcaatcagtt ttttgatttt gtcactgtct
    18181 tcatcacctt tacgagtcgc aataatattc acgtaaggag agttgtcctt ttcaagcaat
    18241 acggcatctt ctaatggatt caaccctgcg ccgtaagcga agtttgcgtt gattgcaact
    18301 aggtcacctt catcattttg gtacgtagaa gctaaaagtg atgggtcgat cgtatggttg
    18361 aacttcaagt tcttagggtt ttcttcaata tcatcgaatg ttgctgtttc cagatctact
    18421 ccatctttga ctttgactaa gccagcatct tgtagaattg tgatgatgcg tccccagtct
    18481 gattcagaat ttgaagtaat gattgttgca ccatctttta attcggaaac atctttgact
    18541 tttttagagt acaagcccat tggttcgatg tggacagcac cggcattgac aaagtcatat
    18601 cctttttctt tgatttcttt gttcaaataa ggaatatgct ggaagtaatt ggcatcaatg
    18661 tcgccttctt ccaaagattg gttcggtaat acatagtcat cgaatttgac gatttcaaga
    18721 tcgattcctt catctttcaa taatggtttc acatgttcca aaatctctgc gtgcggtgtc
    18781 ggtgaagcgc cgactttcaa tgtcgtgtcg tctgctttat ttgctgaatc tttgctgtcg
    18841 ctgttgttac cgcatgctgc taatcctaca gtcagcaaaa gcgctgctgc aaatccaaaa
    18901 attttctttt tcatatccta catcccccta tatcctatta tcttttgtct gttttcttag
    18961 ttaagaaatc accgattcct tgaacgataa atacgattag taaaataatt aaagtcgcaa
    19021 cgaatgtcac tgttagatta ccacgctggt agccttcctg ccaagcaaga cctccaagtc
    19081 cacctgcgcc gatcgcgccg gccatcgcag taaatccgat catcgagatc gttgtaactg
    19141 ttaatccaga aatcaatgcg ggaatacttt caggtatcaa gaccttccag atgatttccc
    19201 agtaacttgc tcccattgca tctgctgctt ccagtacacc agggctgact tcacgaaaag
    19261 caatctctac tagacgggca taaaatggtg ctgcggataa aacaagagct ggaatggctg
    19321 cttgcgcacc tagcatagtg cctacgattg ctttagtaaa tggaatgatc aaaaccatca
    19381 ggatcataaa tggcgttgac cggaaaatat tactgatgat cgatacgatt ccataaatga
    19441 ttcgagcata cggtttgttt ttgcgtccaa tagagtaaag gatcaatccc aaaactaatc
    19501 cgatgatcgt cactaagacc attgagatta atgtcatata tagtgtatca aggatagcct
    19561 gattcatcgt ttccgggttg atttgtttaa aatctagata tgtttctgaa aagcttttat
    19621 ccacgatgga tcacctcaat tcctactcct tgttctttga tgaacacttc tgcttgatct
    19681 aattctttgg tttcaccagt catcaagaca gtcaatgaac caaaggaacc ttctttcact
    19741 tgtttgatct ttccatacac aacgttgaca tcgatgttat agcgtcgaat cgcttgggaa
    19801 atgactggct cgttggcatt tctttcgcta aaggtcaaag ttgccaaacg gccgcttgga
    19861 ttttctttga tgagttcagc taatagttct gtcgaatcag cttctggttc gatgtcttgt
    19921 tgtacaaagc gtttggttac ttcttttttc ggttgtctga agacagtcaa tacgtctcct
    19981 tcttctacga ctttccccaa ctccatcaca gcgactttgt tgcagatttt gcggatcaca
    20041 ttcatttcat gtgtgatcaa gacgatggtc aaattcaatt tcttattgat cgctaacaat
    20101 aagtctaata cttcttctgt tgtttgcggg tccaatgcgc tagtagcctc atcacaaagc
    20161 aggatctcag gatcatttgc caatgctcgt gcgataccga cgcgttgttt ctgtccgccg
    20221 gaaagttgtg aaggatatgc ttgtccgcgt ccttctaacc cgactaaacg caacagttct
    20281 tctgcccgtt ctttacgttt gctctttggt acgccagcta attccagcgg cagctggata
    20341 ttttcaagta cagttcttga ccataacagg ttgaaatgct ggaagatcat accgattttc
    20401 tttcggaatg tacgtaattc tttgtttttt aattttgtaa tatcttgatc gtttacgatc
    20461 acttcgccgc tcgtgggaag ctcaagtcca ttcaggagac ggactaatgt actttttcct
    20521 gcgccagaat agccgacgat tccataaata tccccttttt caacgtttag tgaaacgtca
    20581 ttgacggctt ggatgtttcc gtttttccca ttgaatgtct ttttgacatt cgttagttgg
    20641 attaatgaca tgccattcac atctttctct tatgattttt caaacaaaaa agccttcgcc
    20701 ctcttgcctt aagcaaaagg acgaaagctt gaacttccgt gttaccacct tgatttaccg
    20761 ttacttcgca ataacagcct cttccagtac ttggacttag cttctttccg tatactgtcg
    20821 cgctgtaacg ggcactcccg tagtaagttt gcttacgctc gcttactctg ctctaagacc
    20881 atcttccatc tcattttcgt tacccatttt cagcaaacag ggctctcttc aaacgtccgt
    20941 gacatgtact cttcttttca aagctttctc gtttcatata ggattgctta tgattatagc
    21001 aactggaaga atcagtgtca acttttttca agtgattttc tgattttata attcgtcaaa
    21061 ttcttttgga tcaacatctt tgaaacttaa gatccacgca ttcatttcat ctgaatcatt
    21121 caagatattg atatcttcac cgattttatc attaacagaa gaaactgtac ccgttagcgg
    21181 gctggcaaat tcattgaccg ctttttccgc ttcaacttcg atcagaggtt catctttttt
    21241 tagcgattgt cctgcttttg gcagattggc aaacgtgatg tctccaagtt cttcttgcgc
    21301 ttcatttgtc aaaccaacac agtattcttt tccattaaat aagatccaaa ggttgtcctt
    21361 tttcttcaaa tgcttgcctg ccattttata tcagttctcc ttcataaatt ttttcattga
    21421 atccaattgc aacaagctgg tcctctcgta agatcagtgg tcgcttgatc agcataccat
    21481 ctgtcgataa gacttggctc gcttttgaag ccgtaaactc gcctacttga tctttcagtc
    21541 caagttcccg atatttcatc ccgcttgtgt tgaacaggcg acggattggt acttgactcg
    21601 tttctagcca agtctcaatc gttgaggcct tcggtacttc tttgatcata tcaacggttt
    21661 gataactaac gccatgctgg tctaaccacg ctttggcttt tttgcatgtt gaacatttcg
    21721 ggtaccagta aaaggtgtac aattttctta cctccttgaa gatcttgcag gtgcttcatt
    21781 ttggtatctc atcgataata tcaacccgat accgatcatg ttccctaaaa tcgaggatcc
    21841 tccctgacta atgaaaggca atgggatccc tgttaaagga agtaatccga tatttgctcc
    21901 aatattctca aagacatgga acagcagcat catgatcaaa ccgcttgcga tataggcgta
    21961 aaattcatta ttcgtatcga aacacacccg aatcatacga tagattaaaa taaaatacaa
    22021 gaaaatgacg aatgcaccac cgataaagcc gaagttttca ccgataacgg taaaaatcat
    22081 atcggactca cgaacaggca catagatatt gcttacgttg aatcctttac caaacaagcc
    22141 accggtacca attgccaaaa gtccttgggc cggctgataa ctcattcctg acgtatcatg
    22201 gaaagggtcc agccatgaat caatacgtgc aaactgataa gatttgaatc caactttata
    22261 aagcaagtct cttccgcctt cagtcgtaac caagaagatc gttcctgctc caacgactac
    22321 tgccaagatt actacaggtg cgatgatgcg ccaagagatt ccagacatca ggaaaatccc
    22381 gccaaagatc gcaagaaaga caagcatcgt tccaaagtca tcttgggcca tgatcaatgc
    22441 taaaaccggt attgtcacga tcaagagctt cccaatcagc caaaagtccg atttcatcgt
    22501 ccgttctttt acttgcgtat tatgtttagt tacgatcagt gccatcatca agatgtacgc
    22561 aatcttcatc aactctgctg gctggaaagt aaacgcaccg aatcgaaacc agtttttaga
    22621 ccctgttgaa gcagctaaat tctgatcata gaatttgagc aagagtccca ttaccactaa
    22681 ccccaaacca taaaggtaag gagtcagttt ccagatccat ttggaattca aatgcatgat
    22741 gacaacgatt gctgccgctc caactaaata ccagacaact tgcatcccta cgcctttcaa
    22801 gactgctgga ttgttcggat catggctaag tgctacatat aatgagagca gaccaatcaa
    22861 acataacaga aagactggta aaatcacgcc ataatcaata cgattatcgt tcgaagcttt
    22921 taattttttt tccatgtttc atcccttttc ttaaaaacca tactgctgat tattatagcg
    22981 gtccagttag ataatggaaa gtctttccat agacatttgg gaaaaattaa ctttccgttt
    23041 gtgcagttgc ttgagcaaat gcagtacaaa agcctactgc ttatctttag tgtatcatag
    23101 aacttttttt gatacatgca attatctacc agctgagatt ctcctcgctg tgagaagttt
    23161 ctgtgctttc attgtatcga aggatctctt cggcaatctc tagcagctga tcagaaacca
    23221 gattggttcg ttggatgaaa taaaaatatc gatggaaatt caacggagtc attggcacat
    23281 tttctggaac agagcgctta gacaaaatcg agcgaccgat tccttccttc agcaacgcaa
    23341 caattacatc gttacttttc acgatcatct tcttatcttg gatattcatc tcttcaaaat
    23401 aacgctgcgt gtaatgaaaa acaccagagt ttgcttctct tacaagccac aatttgcttt
    23461 ttgggtcccc agcgatgact agctggtctt ccatcaaaga gtatctttgt acgccggaag
    23521 tctctaatgg cttttcgata aaaccaaaat cggattcatg tttcgctact tgttctaatg
    23581 attcatgaga attcgtgatt tcgatctcaa aatgcacttt aggaaacctt ttgatcagat
    23641 gtttcatcaa ctgaggcaaa agatagacag caaaggtgtg ggaagcaacg atccgacata
    23701 cttcttcttc attttgttca ttgagcaatt tttgctgagt ttcttcccat tcttctaaca
    23761 aaaccagtgt ccgttcatat aataagtcgg cttgcttagt tggaagcatc tcttttcttc
    23821 cgttacgttg aaaaagatcg atattcaatt cccgctccaa ctgtttgatt tgattcgaga
    23881 ctgctggttg agataaaaac agtacttctg ctgctctgga aaaatttctt gtttcatata
    23941 ctgctcgaaa agtcgttaat aacttaaaca tggcaccact tccataactt tttatgataa
    24001 ttactataaa aactatttga tttaaatata caaaaaacca tctataatag aaatgtacaa
    24061 caaaacagtt gctgatccaa ctagtaactt agatgaagac aatcagctgt ctgcttgctc
    24121 gtaatcatac attcctattc attttacaat gtattcgttc atcttggtac taaaatctat
    24181 taaataaaaa ggagaatatt atatatgaag aactttttga aaattattcc gatcccgatc
    24241 tgcgggttga tccttgggtt agcttccctt ggaaatctgt ttaaagacta tcaccatgtt
    24301 gctttaggca atctaactgg cggtatctct atgattctta tgatattcat ttttgttaaa
    24361 ttgtttatct tattcgaaca cacgaaacaa acgttgcatg atccaatcat cgcctccgtt
    24421 tcaccaactt ttacgatgtc actcatggtt atttgtactt attttgtttc atatgaaagt
    24481 attgcaccaa ttgtcaaatt catttggctg tgcgctgtta tcttccaagt gatccttgtc
    24541 ttttatttca attatcacca tgttatcaaa gctgatcttt caattgaagc aatttatcca
    24601 agctggttta tcatttacgt tggatttggg gtcatcactg taactgccgg gaacttcttc
    24661 ccacttattg gaaaaatttt cttttggatt tcattgatct gctatgcgtt cttattgccg
    24721 atcatcatca aacgtgtctt tttcgtaaga aatatggctc atccgacacg tccgctgatt
    24781 acgatcgttg cagcacctgg ttctctttgc ctaacaggtt atcttaaaaa ctttgctgaa
    24841 cctaattttt atcttgtcgt agcattgttc gctttatctc aagtattgta tttcgttgtg
    24901 ctgtctatgc ttccacgttt gttcaagctt cgtttttatc caagctatgc agcctttaca
    24961 ttcccgcttg ttatctctgc aacagctata tttactactg ttcattactt caacagttta
    25021 ggtatctata gtagcgtatt ggatttctta aaagttgcgg aatgtctgtt tgcaagtgta
    25081 gttgtcttct acgttcttgc tcattacgta cgtttcttaa ttaaagaaca tagcaaacat
    25141 ggaaaatttg tttctaaaaa aatcgcttaa taatcgattc aataggttgt gaaaaagctg
    25201 ttctgcatca agtaataaga acggccaaac aaaaacagct gttcatgttt ttgtactggt
    25261 tgggcttatt accgcagatg caagcttttg aacaccgttt accaagaggt cgtaa
//
LOCUS       DACECR010000038        24574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.038, whole genome shotgun sequence.
ACCESSION   DACECR010000038 DACECR010000000
VERSION     DACECR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.038"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(154..552)
                     /locus_tag="IWO17_001393"
     CDS             complement(154..552)
                     /locus_tag="IWO17_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ0290277.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(549..1322)
                     /locus_tag="IWO17_001394"
     CDS             complement(549..1322)
                     /locus_tag="IWO17_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0290278.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1319..2002)
                     /locus_tag="IWO17_001395"
     CDS             complement(1319..2002)
                     /locus_tag="IWO17_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0290279.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2169..3056)
                     /locus_tag="IWO17_001396"
     CDS             complement(2169..3056)
                     /locus_tag="IWO17_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0290280.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3053..3922)
                     /locus_tag="IWO17_001397"
     CDS             complement(3053..3922)
                     /locus_tag="IWO17_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0290281.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4059..5345)
                     /locus_tag="IWO17_001398"
     CDS             complement(4059..5345)
                     /locus_tag="IWO17_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0290282.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5618..6196
                     /locus_tag="IWO17_001399"
     CDS             5618..6196
                     /locus_tag="IWO17_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0290283.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6196..7125
                     /locus_tag="IWO17_001400"
     CDS             6196..7125
                     /locus_tag="IWO17_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0290284.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7122..8858
                     /locus_tag="IWO17_001401"
     CDS             7122..8858
                     /locus_tag="IWO17_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0290285.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="IWO17_001402"
     CDS             complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="IWO17_001402"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ0290286.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9754..10263)
                     /locus_tag="IWO17_001403"
     CDS             complement(9754..10263)
                     /locus_tag="IWO17_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ0290287.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="IWO17_001404"
     CDS             complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="IWO17_001404"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ0290288.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11564..12655)
                     /locus_tag="IWO17_001405"
     CDS             complement(11564..12655)
                     /locus_tag="IWO17_001405"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ0290289.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="IWO17_001406"
     CDS             complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="IWO17_001406"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ0290290.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="IWO17_001407"
     CDS             complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="IWO17_001407"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ0290291.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="IWO17_001408"
     CDS             complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="IWO17_001408"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ0290292.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16618..17592)
                     /locus_tag="IWO17_001409"
     CDS             complement(16618..17592)
                     /locus_tag="IWO17_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ0290293.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17573..19456)
                     /locus_tag="IWO17_001410"
     CDS             complement(17573..19456)
                     /locus_tag="IWO17_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ0290294.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19474..21699)
                     /locus_tag="IWO17_001411"
     CDS             complement(19474..21699)
                     /locus_tag="IWO17_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0290295.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21814..22848
                     /locus_tag="IWO17_001412"
     CDS             21814..22848
                     /locus_tag="IWO17_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0290296.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22915..23928)
                     /locus_tag="IWO17_001413"
     CDS             complement(22915..23928)
                     /locus_tag="IWO17_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0290297.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24006..24194)
                     /locus_tag="IWO17_001414"
     CDS             complement(24006..24194)
                     /locus_tag="IWO17_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ0290298.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7061 a         5340 c         4397 g         7776 t
ORIGIN      
        1 tagcatattt tgaaaagaaa gtctgatgct tagagtagaa tttcataaaa atttactatt
       61 tcataagtat tcacgagaaa aatacaaaca aaaaagcgga aataacgctc atccgccttc
      121 ttgtttgtgt ttatttcctc ttcaaacgct tgtctatgat tcagactttt ctctatcgtt
      181 attttcatct aaaagttctt gcattccgat aatttctcga tattgatgaa tcttagtgat
      241 accaatgact ttgtctacat caatactaaa acaaatacca ttacctggaa tatcgatgtt
      301 gatgccttga ccaatcgcat ccattacttt attcgtcaaa ctatcgggta ctaatgtcaa
      361 cacaatatct ttttctggcg aaatgtccaa acctaaaaat ttccctgaat catgtacacc
      421 agaacctctt ccatgtaaga tcgttccgcc tgctgcacct gcttcttttg aaaagtcgat
      481 aacatcttcg cccattcctc cgtcaacgat cgtcacaatc atgtgaagat tcaatgctac
      541 aagatctttc atttgctttc ccccaatttt tgcaatcttt ctttttcgat tctgtctttt
      601 tcatcttttc gtttataaat atatcctagt actaaagtgg ataatattgg tgtcaatgca
      661 atcattgcaa ccacaccaaa cccgtcaatg atcggatctc ttccttcaat ttgtgcagcg
      721 attgccacgc tcattgataa aataaatgtc gaacacatcg ggcccgttgc tactccaccg
      781 ttatcaaaag ccattgctaa aaagatcttg tccacttttc gtcctaaaat aaatgctagc
      841 ccgtatcctg ggatcaagaa ataataaaga gagaatccga cgagcaaacg ccacatagac
      901 aatcctactg ctgcgccgat cccaattgaa attaccatca gcatgatttt cgatcggatt
      961 ctcccctcgc tcaattcttc aacctgtttg atcattacat gaatcgccgg ctcagcaaac
     1021 cctactaaga accccattaa aaagccaagg ggaaccagta tataattgta aggcaatccc
     1081 gcaatagcac tgcctaaatg ctgtcctacc ggtacataag ccatattgac tccgtgcagg
     1141 aaaaggatca acccaatagt cgttatcaag aaacctttca taatgtcaaa gaaacgcttt
     1201 ttgcgcatac gaaaactaat gatattcatt aaaacaaata ggattacgat aggtgccact
     1261 gccaaaatga cttccatggt aacgatttcg attccatcta tcacttgctg ccagaaactc
     1321 attggaagat cacccccaaa atcatcacgg caagaatcgg ccctaacgaa gcaataccga
     1381 ccattccaaa actgtcattt tcatttttct tttgtttgat catactagtc atccctccgg
     1441 caagggccaa gataaatgga acagttactg gaccagtagt cactcccccc gaatcaaaag
     1501 caatcggcat aaattcggaa ctggtgaaaa atgaggcgat caaaacgcct atgtagccta
     1561 tagccatcaa ttgataatag gatagcttga atacgacccg aagcagcgca aaggctagaa
     1621 atactccagt gccgacgctg acgatcccaa tcagcaacac tctgccaatc tctccgtcag
     1681 atatatcata tacttgttgt cccaaaactt gcacggaagg ttcagcaatc gtgatcacga
     1741 tcccgatagc aaatcccaaa ctgaccaaaa cggtcaaact tttctttttg atcatatatt
     1801 tccctactag atctccgact tccatcatag aataatctgc tccaaacaaa aagagcgtca
     1861 ttccgatcat catgattgcc gcaccaacca gaaatgaagc gaggtcttct ccttctaacg
     1921 gtgcaaaaac aaacgttaaa atgacaatca ggacagtcat cggcaatata gcagcaatca
     1981 cttctttaaa attttttagc atgtatcctc ctcctttact ctttaagttt accttaaaat
     2041 gaaagagttt tcgagcacaa acttaaaacg ttatcattta atttttctca acataaaata
     2101 acacacgtcc caaaagtatc tttttacttg gaaaaatgac tttgaaggaa cgcgcttatt
     2161 gtgttttttt attttacaga gccgatcatt ccttgaacga attgcttttg caaaacaaaa
     2221 aatacgatgg cagttggtaa agtagcgatg actaatccag ccatgatcat tccatagtct
     2281 ggtgtatagg aagcacccat agcagataaa acaagcggaa ccgtccgttt atcaggagat
     2341 tgcaaagcta ctaagggcca taaataattg ttccagctac tcatgaatgt gatgattgca
     2401 gccgcggcat aggtattttt agccgtcggc atatacactc taaaaaaaat acttagttct
     2461 tttagaccgt ctaatcgagc ggcttctact agatcttttg gaaaagattt aacattttgt
     2521 cgaaagaaga agatcaaaaa agcagtgcta acagatggca ggatgacaac caagaaactg
     2581 ttgattccca aaaccgttcc gttcaattgt gaaaacattc gatataaagg gatcatcaat
     2641 gccgcaaaag gaaccatcat cgatagcagt agaatattga aaattaggtc ttttcgttta
     2701 ctttgataaa tctcaaatcc atatcctgcc attgacgaaa tcagcaatgc aaacacagtg
     2761 gtcaccaaag cgatcatcgc tgaattccaa agggaacgag taaaattcag ctcattggaa
     2821 aacaaatttt gaaaattgat gatcaagtta ctgccaatct ttatttttcc cgatgtgata
     2881 tccacgggtg tattcgtcat tcccaaaacc atccagacaa aagggaaaat ggaaataaat
     2941 gataggatgc ttaatgctgt atactttaat actgacccaa ttgtcgaacg aagttctaat
     3001 gcttgttttt tgacgttggg gcgttcgatt gtttcttttt ctaccatatt tttcatcgac
     3061 tttctcctcc tactttcgtt tggattatcg acaatatcac aataaaggaa acaataacaa
     3121 aggaaactgc tgccgcatat ccaaaattag gcgtgaattt ataactcaaa ttatagatgt
     3181 attgagatag ggtggtcgtt gcatttgctg gtcccccgcc agtaatattt tgtacttcat
     3241 caaaaagctg caatgtcccg atcgtcgaag taatggaagt aaacaaaatg attggtttta
     3301 aatttgggat cgtcactttg ataaactgct gccatttaga agcaccgtca atagatgctg
     3361 cttcataaat ctccgaatca atattttgca tgcctgaaag gaaaaaaatc atattgtatc
     3421 cagtccaacg ccaagtgatc gaaagaatga tcagaactct cgcccaaatt ggatgagtca
     3481 accagccaat cggtgtgtca atcataccaa tgcccaacaa caagttgttt acaaggccag
     3541 attgagaaaa tagacttttc atgatcagag aataactgac catcgaagtg atacatggaa
     3601 gaaagatagc ggttcgaaac aaccctttat atttcaactt tttatcattt aaaaggttgg
     3661 aaataaaaag tgctagaaac aacatgatcg gtacttgtac aagtaaatac aggaatgtat
     3721 taaaaaaagc ttttttaaaa gttgcatctg tcaacatccg ctgataatta cctaacccgt
     3781 taaatgtcat attgacaggg ggaccacttt gaaaagacgt gatcaaagcc aaaaccatcg
     3841 gaataaaaac catgattgat atcaaaatga ctggcaagat taaaaaagca tgcccgtttt
     3901 tgaataaatc tttctgcttc attcttttcc ctccaattct atcattgatt atctacatga
     3961 aaagacaaga aagacaatat ctgtaaggca gacgtgatct tttttgtctt ttcgtttgtc
     4021 tgatgaaaaa gcgaagaaat tcactttttt accaccgatc aattagccaa ttgtgcttct
     4081 acttgttttt gtgtatctgc tagtaccttg tctgtctctt ctccattgat gattcgatga
     4141 agagcttcag ctacgactgc ttcaattgca taggtctcat gtccgtaatt cacttctggt
     4201 atttcagatg tccatgttga aaaatcgtca aagatctttt gaccactgta aaattcagaa
     4261 gcttcttgat aagcttcttg ttcattcgcg ctaagcatcg tagatactag tccgatttct
     4321 ttcgcaagag tgcccatcag ttcttcattc gaagcaaacg ttgcttctag gaaatctttc
     4381 gctgaatctt ctccagctac tcctttgatc acataccagc cagcgccgcc aaggttagaa
     4441 gcttgcgctt tttgcagatt ttctggaagt gcaggtattg gtgcaatctt ccattttcca
     4501 gattggtttt ctgctccttg gatcgttgaa gaataccaag cgccagttgg gctagaagct
     4561 actgctcctg attgtatcgc agttactcca gcattccagt cagaggtttg ttcgactaga
     4621 tcttctttca aaagagtggc aaacaattca agaccatact ttaaagatgc attgtcttgg
     4681 atcgtgactg tctccccatc ttcagatgta taccattctc ctgcctgttg cataatcatc
     4741 cttactcgac caaggtcaga aggattgact tcagcgattt tctttcccgt cttttctttg
     4801 acatctcgcg ctacttgaat atactcgtcc cacttcagat tctccatatc ctcttctgta
     4861 taacctgctt ctgccatcag atccgtacga tagaagtttg ctgttacgcc cgaatcgaaa
     4921 ggaacaccgt atattttatc tcctactttg tttaccgcaa atttatacgg tgcaaaatga
     4981 tcttccttta caatagcaga caaattagcg aatgcatctg gataagaagt caaatagcct
     5041 tgtatcctat aatcttcaat caagacgata ttgggcaatc cttctgtatt cccgctcgct
     5101 aaagcggtgt tcagtttctg aacgatatca tcctgagaca tcgtaacaac ttctatctct
     5161 gtttcttcgt tctcataaac tttttttgct tcattcactg ctttgatatt gaatgtctca
     5221 tcccacgccc agacagtgac tttttcttct ttgtctgctg tactactagc acttccgcat
     5281 cctgttaaga aacctgatgc tgccgctaaa caaacgactc cagcaattaa ctttctgatg
     5341 ttcattttta tccgctcctt ttccatccat tagttgttta cgtttgctac attcatcata
     5401 ccaaaatttg aaaacggttt ctttattatt cagatacttc aaaaatttct cctaaaataa
     5461 taaaaaaata caaatcaact atgacaaaag gaataatctt agcatctaga ccaaagatcg
     5521 aaatggtgag gtttctttaa aataaaaata tccttgcatt ttctcctaga aataagtatt
     5581 tttaaattac aagactattc ttaaggcggg taaaaaaatg ccaatacgcg taattcttat
     5641 cgatgatgaa cagacgattt tagatggaat gaaacagttg atcaattggt ctgagcacgg
     5701 gtttcaatta atagggactt tttccagtcc gctgaaagcg cttgattttt ctttggtaca
     5761 tgcaatcgat atcgtggtca cagatgtaat gatgcctgaa ttaaatggaa tcgaattatc
     5821 gcggctatta aaacagcaac aacctgaaac acagatcctt atcctctcta gctatgatga
     5881 atttgatatg gttaaagatt cttttaaaga aggcgtcgct gattatcttt taaagcctcg
     5941 attgagtcct gatttttttc taaattcctt aactgccgtt tcacaaaaaa tcaaaaaaaa
     6001 gcccaacaaa agcccaatca ccaatcgtta tgagcagatc tgcgagaatc tttcacagca
     6061 gattgctgga caaactcgtc cattgcttga ccctcttctt tttgaacaca ccttgttttt
     6121 ctgttatata ccgaaaaacg gccattcagt tcaaaacaag gctctaaaca aaaacagtcg
     6181 atcacccatt cattgatgga tagcgacgat tcgtttacta tttatcctgt ttctaccttt
     6241 tcggaagaat ccggctatgt aatcaatact gattccaaag atacacttag gaagtttgtt
     6301 caaaagtttc gtcaagaagt ttttccaggt gatttttttg ttgtttctga ttcttttgca
     6361 atccaagata tcaatgaaat attccaagag atcagaaatt gcaccaaagg acaacgtttc
     6421 tatcataagg gaaagaacct tgcttatcag catgaacttc ttccgttatt aaccaaccac
     6481 gagcagcaag caagtagtta tttaacaaaa atcgttcata aagactacgc tttagctgtc
     6541 aaagaacttg agaatttctt cacgacgata ttggaacagc ctattgatcc agcttatttg
     6601 aaacatgagg caatcaatcg tttctatgca ttactcaacg ctttggctga cgagcatgtt
     6661 gggaatgaag aactttcttt gctcaagatc gcccttcctt ctttattagc cgatgctgag
     6721 tatttcgaag atttttctat tcttgtcaaa gaaacacttg agaaactcac ccacttttct
     6781 gcaaatgcaa aaaaagacaa tttcgatatc ttgactttga tctacgagtt cgtccaggaa
     6841 aattatgaga aagaaatcaa tttacaactt ttatccgaaa aatatcattt ttcatatagc
     6901 tatctctcag ccatctttac tgaaaaattt ggtatcaact tttcaaaata tctaaaaaaa
     6961 gtccgtatca gtaaagccaa gctctttttg acggaaacac ggcttacttt gacggaaatc
     7021 tgtgagaaag tcggctatac cgaactgggt tatttctcca gagtttttaa agaagagaca
     7081 ggaatgacac cttctcaata ccgtaaagga acttttgtaa aatgagaaaa aaaatcaaag
     7141 agcatgaact ctttgccaaa cttctgttca ttttgttcat cggtcttttt ctgcaagcca
     7201 tcgtcatttc tttgtttatt tatcatcgat caagggatgc ctatatccag ctgttcaatc
     7261 aatcgaatga tgtggtacta aaaaagattc aatctgaatt tgaaagtttg aatgatacaa
     7321 tcgagaatac attagccgct tttgattcta atccagctgt caaaagctat ttttccaatg
     7381 atccagccca acatatggaa cagcttcagc aactaagaac aatccaaaaa atgaatgact
     7441 ctctttcaaa aatccatcca atgatcgatt acgatgtatt gatctttgga gaaaatggaa
     7501 gaacttttgt cggaaacgac atgctcactg ccgtatctgc cgattctttt tttcaatctg
     7561 cgattgctca gagagtgaat gaacgtgctg ctgatacaca aatgcttttt gcacaccacg
     7621 gattgactct tcgcgataag aaagcaccca gcgtcttctt tgtcaaaaaa ctgaaaaata
     7681 cattgaatca cgtttacgga tatgctgttt tatcgatctc atccaagcaa ctagcgaatc
     7741 tttttcaatc tgtagtaaat cctgagatat cgaagatctc cttcatgaac gaagaacaga
     7801 cgatcatcgc atcaaatgaa gaaaatacaa ttgggagccg atcaatgatt ttcaaaaaac
     7861 taacagtagg tgaaacaacg atttcaaatg gacagcagtt gacccgtctt cctctgtacc
     7921 gccaggattg tacattactc agtcaagtcg atcttcattc tttatctaac caaatgggcg
     7981 tgatttttcc gattattttg tataatatcg tgacacttgc ttttgctggc tcattggtat
     8041 ttatgtattt ggatcgacac acaaaatcga tctaccgctt gattgattcg ctaaaacggc
     8101 tagaggaaga tggcttaaaa gctactgttc ctgtacaagg aagccatgaa gtccaattgc
     8161 tagggaatac gatcaatcaa ttgctaagta atgctcagca tcattatgaa cagacgatcc
     8221 aagacgaaaa gaagaaacgt tcgcttgaaa tccaaactat gcaagcacag atacagcccc
     8281 attttattta taatacgctg actagcttga aatttttgat ttggcagaaa aaaacagatg
     8341 aagcagttca aggaatcgac agtttcatta ctttactaag aagtacgatt gggaaaaaag
     8401 aagaagtgat tccagtcaag gaagaaatca aaagcgtaca aagttacatc gatcttttga
     8461 gtatgcgata tggcgaaaga atctcggcta aaataatgat tcctgatgaa ctgcagtcat
     8521 tatctatgcc gaatatgatc cttcaaccaa ttatcgaaaa cgcctatctt catgcttttc
     8581 acaataaaac gcaaggattc atcacggtgt acggcaaaac aaaaaatcag cttcttttat
     8641 tcgaagtgat tgataacggc gatggcttcg atactgaaaa taccaaacaa aaaaatgatc
     8701 atttttcagg aatcggcatc agccatgtag atgagcggat acgcctaatg tatggcgatg
     8761 catatggctt aacgatccaa tcagtgattg ggacaggaac gactgtaacg atttgtctac
     8821 cgcttcttcc taaaaatcaa tcagaatatc aaaaataaag aatagccaag cgctgatttt
     8881 tttattttaa gttaaataaa tcaatcagta ttcttgactt tcatctacgc gtctcttcta
     8941 ctgtcagcac tggataacag cccaatattt gactttctcc tccgagcatc ttgatttctt
     9001 ggagcgcatt ctctactaat gtcattggac ggtccaacaa caaatcgatc acgaaaaaat
     9061 attctcccaa gctagttttc aatggtcggg attcaatctt acttaagttg atttcccgcc
     9121 agccaaaagc tgccagcatt ttatgaagca tccctggccg attagcaggt agtgtcaaga
     9181 tgacactcat ttttactgga gctgactgct ggctggtcat ctttcggtca ccgacgatcc
     9241 aaaaacgtgt ctggttcaac tcattatctt ggatattctc agctaagatc tccaatccaa
     9301 catgctgtgc tgtttctaat gaagcaatcg ctgctgcgtc ttctttcgga tgttctgcta
     9361 catacattgc tgcagcagtt gttgattctg ttgctactaa aggaacatta ggataatgcg
     9421 tttctaaaaa ctgttgtgat tgtgctagtg cttgtggatg tgaaaggatt tttgtaatct
     9481 ttgttgctgg attgcctaaa agctgctgct tgataggaag aacgatttca cttttaactt
     9541 cgacttctgg atgtttggaa agaagatcga ttgtgtgatg aacagctcct tccaatgaat
     9601 tctctacagg aacgacagcc agatcgactt gtttgcgaaa caaagcgcgc aaacaaacag
     9661 ggatggaagc atacgcagtc aattgttctg taggaaatag ctgacaagcc gcctgataag
     9721 tgaatgaact ttctggccct agatagctga cgttcatatg cttttgatcc tttctataat
     9781 ggtatcgatg atcttttctg gtgtttgatt ggtcgtgttg attcgatgtg tcgcactttc
     9841 ttcataccaa gaaagtcgtt gtgccaataa tgcttttatt tcttctcttg tttttccaag
     9901 agctaaaggt cgaatagacg tttcatcttg ttggatacga tcaactagac agtccgcttc
     9961 tgcctctaag taaagaacaa tcggctgtgt ttttaaaaaa cgccgatttt ctgcactttg
    10021 gacaatccct ccaccagtcg cgataatttt gtcttcattt gacagctttc ttaaaatatc
    10081 tgactccact tttcgaaagc ttttttcacc gtaatagtga aaatattcgg caatcgacaa
    10141 ttggatatgt tcctcaatct ttgtatctaa atctacatac tctttttgta aacattgagc
    10201 taacccttta ccgattgttg tttttccagc gcccatgaaa ccaatcagca aaatgctagc
    10261 catgagcttc tcctcctgac acaagttctg ctaaatcgtc aaaaaatgcc ggataagaga
    10321 ttgaaacagc ttcagctcct tctagttcgc aaggtccatc agctatcaaa gcagctactt
    10381 gcagcatcat accgattcga tgatcgcctc tgctggacac ttttgcgcca tgcagaggtg
    10441 tcggcccatg gatgatcaga ccgtctgctg tcgcttttat ctttgctccc attttctgta
    10501 attcttctgc caccgcatca attctgttgg tttcttttac ttttaattct tctgcgtctt
    10561 tgatgatcgt gattccttct gcttgagtag ccactaaagc tagaatgggc aattcatcaa
    10621 tcagacgcgg aatgatttct ccaccaatcg ttgctttctt caaatgactc gtcttgacac
    10681 ttaaatcagc cgattggttc tgttcattgt gattcgtctg ggtaatcgct gcgcccatct
    10741 cctccaacac atctaagata ccagttcttg ttggattgac cccgacattt tttaacaaca
    10801 gttggctttc cggaacaaga agtcctgctg ctagaaagaa agcagctgat gaaatatcac
    10861 ctggaacagt tatctgttga ccagttaatt tttgcggtcc ggtcactatg atcgttttat
    10921 cttccactgt gatccttccg ccaaattgac gaatcatttc ttccgtatgg ttacgggaac
    10981 gttctttttc aacgattacg gatgtgcctt ctgcttgtaa tgccgcaaac agcaaagcag
    11041 atttgacttg cgcactggct acaggcatat tgtatcggat aggtgataaa gagcgggttc
    11101 ctgtcacagt gattggtaga tactggtcat tttcttctcc ttgcaagtca gcgcccatct
    11161 cgcgtaaagg ttccatcact cgccccattg gacgtttaga caacgaagca tccccgaaca
    11221 gagtagtcgt aaaaggctgt ccagccaaaa tacccatcaa taaacgtatg gtcgtaccgg
    11281 agtttcccat gtcaattgat tcggttggtt ggacaaatga gtggctaccg cgaccatgaa
    11341 tcttgatcac ctcttcctct tcttcgatct cgacacctaa ttgttggaag gcatgtaaag
    11401 tactcagaca atcttctgcg cgcaaaaaat tttgtatagt cgtcgttcct tcagcaattg
    11461 ctccaaacat gatgctgcga tgggaaattg atttatctgc tggtatccta acagtccctc
    11521 ttaatccatg tatttgttgt aataattgca tttatccagc ctcctatttg atccagcaat
    11581 gataactggt attagacata atgattcttt ttgcttgtaa caaatcctgt tggtttttaa
    11641 aactgatttg cagtattcct aaaatatctt ccctggtctc ttggatcttc aaattaatca
    11701 aagaaactcc cgcttttccg atcagtccag ttacttctgc aataacgcca ggttcatctg
    11761 gcacatcgat aaataaatca aaaaaggcag gaatcgcgcc ttgcttatgg acaggcaact
    11821 ggtctcgact ttcttttgcc cgattgaaaa aagaaaagat ttccgattgg ttctcagtca
    11881 tgatccattc tgctatttgt gtcatttctt gttgccaatc tgccaatcgt tccaataata
    11941 tctgcttatt gctcaacaaa atatctgtcc acatgacagg atctgaggaa gcgatcctag
    12001 tgatatctcg aaagcctcct gctgccagtt gtttggctcg cggatactct tgattgaatt
    12061 gttctgcctg attgactaag ccagctgcca gaatatgtgg aaaatgactt agcattcctg
    12121 ttatacggtc atgttctctt gctgtaagag ttacatattt tgctttggtt ccgcaaaata
    12181 gttcttgcag tttttcaaca tcttttttcg tcttttccaa aggtgtaaag atgtaatagg
    12241 cattttcaaa tagtttagca tctgctgcct gtatacctga tttatgagaa ccagccattg
    12301 gatgtccacc gacaaaccga agatttttct gctcagcgga tgtcacgact tgctgcttcg
    12361 tactgctgac atccgtaatg atgacgtgat cagacaagtt gattctttca aggacatcaa
    12421 gataagctaa ggttgtacgg acgggaacgg ctaataagat aacgtctgct tgttctgcac
    12481 ctgtttcaaa atcacttgca acgtaatcaa cgatctgctg ttctactgct tgctcacggg
    12541 tttttgactg actatcccaa ccaactacta tcagttctgg attggtctga cgaatacaaa
    12601 gtgccataga agcaccaatc agtccaagcc ccacgataaa aacatgttgt gccatcttat
    12661 ttgccccctt tttagtaatg tttcaagtag tcacggtact ccttcactgc agctttcaac
    12721 tcaacaagcg aatcacttgg gaattttgac aatagctctg tcgctaccac agtggctaca
    12781 acattttccg caacgacgct agctgcagga actgctgtac tatccgaacg ttctacactt
    12841 gctttgtaag gttcttttgt atcgatatca acactttgaa gtggcttgta caatgtagga
    12901 atgggtttca tgaccccgcg aatgacaagt ggttcgccat tggtcattcc gccttcgaag
    12961 ccgcctagat tattggtacg tcggaaatag cctctttctt cttcccatag gatctcatcc
    13021 atcacttctg agcctggtaa tgaaccagaa gtgaatccga ccccaaattc tacgccttta
    13081 aaggcattga tactaaccac agcttgtgcg attttggcat ctagcttcgt gtcccattgt
    13141 acgtagcttc ctaatccagc tggaacgcct ccaacgagca cttctacaat tcctccgatc
    13201 gtatctccat ttttctttgt ctgatcgatc aaatctttta ttctttgctc aactgctgga
    13261 tcaacaactc gtacttctga cgcttcagaa atttctttga tttcttgaat cgagatattt
    13321 ccaggtattt ctgcttggat tccaccaaga caaatcacgt gtcctgctac gatgatgtca
    13381 agttcttcca gaattttctt ggcaattgcg ccgattgcta cacgcatggt cgtctctctt
    13441 gcagaggacc gttcaagcac attccgaaga tctcgatgat ggtatttcat accgcctacc
    13501 aaatccgcgt gtcctggacg cggacgtgca actcgccgca gtgattggtc tttttcaggc
    13561 acttctgcta cagacatgac agttgtccaa tttttccaat ctttattttc taccactaaa
    13621 gtgacaggtg aaccaattgt tttgccgtga cggatgccag acgtgatccg aacacgatct
    13681 ttttcgatct tcatccttcc tcctctgccg taaccagctt gtctgcgagc gagttcgtga
    13741 ttgatttctt cttctgacag aggtaatcct gctggtagtc cttcaataat ggctgttaat
    13801 tctggtccat gggattctcc cgcagtcata taacgcatct tacagtcctc ctaagtaatc
    13861 tttgaatgtc tctgtgggaa caggaacgat ttttgcttgc ccaatctttt ctagcaagat
    13921 gatattcaat tggcttcctc ttgctttctt atcatgggtt atcgcgtgga ataattcatc
    13981 tggatgactt agatcgtacg taatcggcag atggaatttt tgaagtatcc tttctaaagc
    14041 agctgtcgat ccagttggag acaagccgat tttttctgca tgagaagtga tggctaccat
    14101 tccaatagct actccttctc catgactgac taccccatat cctgctgtat tttcaattgc
    14161 atggccaatt gtatgaccaa aatttaaaat aagtcgattt ccttggtcaa agacatcttc
    14221 ttccactact tttcgtttga tttccaacgt agcagtaatg attggaacag catgttccag
    14281 aagatctttt tcgtctttca gctcgttcag ctgtttccaa agcgttgcat ctgctatagc
    14341 tgctgatttg atgatttcag cgattccttc tcgtaaacga cgtaccggca aagtttttag
    14401 aacctcagga tcaatcaata caccagctgg ctggctaaat gttccgacaa ggtttttcgc
    14461 tttgtttgta ttgactgctg tcttcccgcc aatactagaa tctacttgtg caagtagcgt
    14521 agtcggaatt tgaacgaaat ggatcccacg catgaacgtc gaggccacga atgctgctag
    14581 atcacccacc attcctccac caagtgcaag tatcccatcg ctgcgggtaa aatcattatc
    14641 tgctaaaaaa tcgtataact cagctgcttg ttctaaagat ttactggtct cacccgccgg
    14701 tacgattgaa aggactggtt caaaattagc ttgtctcaat tggaacatga ttttttctgc
    14761 atatagtgga gcaacgtttg tatcagtgat cacagctact ttttgaggtc tccaaatcgt
    14821 ttgaagccat gatccagcct gttctagtgc gcctttttca atatgaatat cgtatttttg
    14881 gttatcaaca tgtacagtaa gcattcgttt cacttctttc ttttaaaaag ctggaattaa
    14941 tcgagtgtct gaatcttttc cattgcttct ttcatgatcg caacttcttt catcatgcgt
    15001 gagtaagtct tttcattcaa cgattgggga ccgtctgacc aagcatttgc aggatcagga
    15061 tggatctcta cgatcatgcc atctgctccg cttgccacac ccgcacgagc cattggtggt
    15121 acaagttccc aaattcctgt accatgactt ggatcaacaa tcacagggaa atgagttaat
    15181 tttttcatca atgggacagc acttaaatca aacgtattac gtgtcgctgt ttcgtaagtc
    15241 cgaatcccac gttcgataaa aatgacagga gatttgtctt cgactgcaat gtattctgca
    15301 gcattaagcc attcattgat cgtaccggaa attccacgtt tcaatccaac aggtttacct
    15361 gttttaccga cagcagatag cagtttaaaa ttttgcatat tacgtgcgcc aatctgtagg
    15421 atatcgctgt actcagccac catatcgata tgcccttcat ccatcacttc agtaatcact
    15481 ttcattccaa attcatcagc cgcttgacgg atgtatttca atccttcttc ttctaaacct
    15541 tggaaagcat atggagaagt acgtggtttg aaggcaccac ctcgcagaat ttttgcgccc
    15601 ccggctttgg ccattcgcgc acattctctg atttggtcta atccttcgat agaacaagga
    15661 ccagccatcg tcacgaaact gccgtcacca atttttactc cgtctacatc cacgactgta
    15721 ttgccaggat ggaattctcg agaagtcaat ttgtatgtgt ttaaaatacg cacagcattt
    15781 tctactcctt catagcgtgt aaatgctcct tcttgtaaac ttcgcgtatc accttttagt
    15841 ccaatcacta ctcgttctgt tccttgatcg attgagactt tgaatccttc tttttcgata
    15901 tgatcgacga ctttttttat ttgttcttcc gttgcttctt ttttcataat tactatcatt
    15961 gtttttcccg ctttcttttg tttacttatc tatcttggct gattttctct actttttcaa
    16021 ggtggtttta cattaattcc tactaaatga ccgtgtcctg ttgttctcat tttcgctcct
    16081 tctttcctac ttatgaccca gataatcaac aaaaaaagat gtccctgtag gaaattgcct
    16141 ctacagtgaa catcttcaac gagccttaaa aagcgctgca aaaatataaa atagccacta
    16201 tagatgaatt tctctacgtg gctgtaagtc gtgtttaaaa atctttagcc atcatagata
    16261 caaacctggt ttgtttgtac ccacgaagtc atgaatacaa acctatctaa aatagctaaa
    16321 gtaatacgta ttcagttttg taagttttct agatttcata aaaaatcgct ccaaactgat
    16381 gtaagaattg tacctagtat agacataaaa aaatcgactg tcaatcattt ttatgaaact
    16441 ttttttaatt ttctgatagt taactttgtt tttcagaaaa ataacaattg gttttattga
    16501 agaaaatagt catgaacaaa agtatctgag aactttttta ctctcttgtt catgacatat
    16561 agaataaagc agctttgtct tgtaaactcc gtttttcaaa taataatcta aagattgcta
    16621 ctgaataata aagccgattt catggtcctc ttcgctgctg attcggtaag gtgactcaac
    16681 atccgctccc cagctattga tacccccgac tcctcttact tttgcaaaaa tcgtaagaac
    16741 cgttcgtctt tctggcggta gttcttctat atgtgttgcc tgttccagtt cttctgcttt
    16801 gaatggtaaa gcggaaaaag caaatggcgt atcctgctgt tcaaatcgca acgaaaaatc
    16861 ttcttgatct ttatccgtat tatccaacgt ttgatttctt gttagttcta cccatttcgt
    16921 ttccatatgc atgccgcatt cttggggaac aaggtatggg gaaacgggga gtcctgccac
    16981 tttataaata ccttcgacac caccagccat ccgatccgga tatgtttccc ctgataggcc
    17041 ttcatactca aaaaattttg ctttcgttgg cataatgaac cttaaaccca atgcgggtaa
    17101 ttccggtaat cctttttgtc cataataatg catattgaca agcattttac cctctccagc
    17161 gatttggtag gaaattgtca cttgggtttt tggtactgtg ttgatttcca gtgtaaaaat
    17221 gatttcaacc gtttcagcgt attcattact tgaatattga ttatttactg gagcaatcgg
    17281 caaatcgatc tttttattgt ccaccagtac ttctatttct ttgacttttg gatacaatcc
    17341 ggctcctaac cacataccgg atttaaatcc aaatccgcta cctctgtcat tatcggtcgt
    17401 tgctcgccaa aaaactggaa gtgtttcccg gtatagccat tcttttccgt ttttattgag
    17461 cgattccagt ccgcctcttg tataagaaaa aatataatgc gtattccccg atttgacacc
    17521 taatgtacag tcgccgaaga cgattgcaac tttttccgta tccatatgac ttttatttac
    17581 tgtatttttc ataataatat cttacctcct gcatggcggg tttttcttgt ccatctgcaa
    17641 aaacgatccc attccctgag aattcatagt cacttggttt atcgtcaaag tcaccgccat
    17701 agcgcatcac ttctctgcct gtgattggat cagtcacttg gatcgcctga tctttaaaat
    17761 cccatatata gcctccttgg tacatttcat aacgatcaag caacgtcatg taagaatcca
    17821 ttccacctaa cgaattcccc atgtcatgca tatattcaca taagatgaac ggtttttccg
    17881 ctttattttc aaggtagtca atgatctctt ctggtggcgc atacatgcag ctttttacat
    17941 ctgaaatcga atcttcgaat gctggatttg gaaaattccc ttcataatgg acaagtcgat
    18001 cggggtctac ttttttgaag taatcgttca tttgtcgaat gttttcttcc gcataagatt
    18061 catttccaag agaccaaaaa agaatacttg gatggttttt taatgtttcg aaattcgttt
    18121 ttgctcgatc aagaacagcc gcctgccact gaggtactgc acctggtacg ttgtaagaag
    18181 gctcaaatgc gcccattttt tgccatgagc catgggattc gaggttcgtt tccgccatca
    18241 catggattcc tgcttggtcg cataaataat accaagtgat ctggttgggg taatgagatg
    18301 tccgtacgcc gttgatcttt gcatttttta tgatagccaa atctttttcc atatcttcta
    18361 tagtgatggc gcgtcctttt ttcggatgcc actcatgtcg atttaccccg ttgataatca
    18421 tccgttttcc attgaagtag atgattttat ctttgatttc gattttacga aaaccgaagg
    18481 aatagtcaat atactccgtt actctttttt cttcgtctat cgtctccaat tcaagcttat
    18541 ataaattggg ctgaaaatgc ccccacagtg ccacattttc aaatccatcg atgcgaatcg
    18601 tcatttgctc ttttaacgga tattgctgtt gatagatttc cttcccatca ggatctagta
    18661 tcgctacatt cagctgactg tttttggcta cttgctcaaa agacaaatct acttcaagtt
    18721 tgccattttg acatttgtca tctaagcgag gtttgatcca aagatcttct aaatggtttt
    18781 gtcctcgagc aactaagaag acatctctga agatcccaaa aaatcggaaa aagtcctgat
    18841 cttccaagaa tgcagctgta cttcttttgt aaacctccac agctagaata ttttcttctt
    18901 ccaaataagg tgttaaatca aattcagacg gagtgaacga atcttcggca tacccgataa
    18961 aatgaccatt caaccaaata aacattgctt gttctacacc agcaaaatga atcgagattt
    19021 gttttccctt tagaccttct tctaatcgaa aagtcgtcaa atacgaacca accggattgt
    19081 ctttagcagc tgaaaagatc ccttccgcct ctttctcagg gcttgtcgtg aaagggggac
    19141 ggcgaaatac atgtccctcc cacggataca tcgtattgat gtaatggatc ttgtcataat
    19201 ttgctaattc aatatgacta ggaacagtga tttcatcaaa agaacaggca tcaaaatctt
    19261 ttttataaaa tgtttttggt cgtaccgaag gatttggcga ataacaaaaa cgccatgatc
    19321 catttaaaga ctgtttaagg gaggatgtgg tttctacttc ttcccttgtt gcataacaaa
    19381 catgatcgct atgtgcattt gattggttca ctctaaaaac ttgtggatca tctaaccatt
    19441 ttatatttgc tttcatactt ttttccttct ctctcataac tttttgaaat gaagcagttc
    19501 tgtttggaaa tcaccttgaa aatcggggaa gtaaagtcca actgtcatca attcggcccc
    19561 cgaaaatatc cgcttcgagg tctgttcctg ataaagtgcc tcatcatcta accccatcag
    19621 atatacttca tggaacgctg gttgagctga agctagtatc ctccctaaaa acacaatagc
    19681 ttcatcttgt ttaggagaca caaacatcca tgcggcaata ttttcttcaa aaggactcgc
    19741 caaacgataa taatggccat actggatcac cggacgaatc gtttgatagg atataatttg
    19801 gtcttttact tgcttttttt cttcaggaga cagctttgtc aaatccagtt cataaccaaa
    19861 aacagcgctc atagctgttt ctcctcttgt tttcaacgaa gtagatctgt cggtctgatg
    19921 atttggtaca gctgatacat gagctgtgat ggacgaaatc ggataagcaa ggcttgttcc
    19981 atattggatt ttcatacgct ccactgcatc cgtgttatca cttgtccatg actgcggcat
    20041 ataataaata aacccggcat caaaccgtcc acctccacca gaacagcctt cccataagat
    20101 ttccggataa tcagttgtca aatgttcaag tagttcataa agcccgagca tataacgatg
    20161 cgctacttct ccttgtctct gtgggtcaag ggtaatcgaa tagacatctg aaagcgaacg
    20221 gttcatatcc catttgatgt agtcaagagg aatcgtatct aaaatctttc tcatttgttt
    20281 ctcaatcgtc tgccgaacat ctaaccttgt aaaatccagt acatgttgtg atctggaagc
    20341 agaaggcatt ctacctggct cctgcataag aaaatcagga tgtgttcgat acagttcaga
    20401 atcaaccgag atcatttcag gttcaaacca taaaccaaat ttcaatcctt tgctatgagc
    20461 ataatctgct atttcacgta atccatttgt caacttgcct tcatactcga accagtctcc
    20521 taatgaagaa ttgtccgcat ctctttttcc aaaccagcca tcatctaata cgaacatttc
    20581 gatgcctagc tcctttgctt catcaatgat cgccttgatt ttttcactat tgaagtcaaa
    20641 gtaagtggct tcccagttat tgaccagaat cggccgttca gcaaattgat gacgacctct
    20701 tgcgattctt tcacgtagca actcatggtg gatctgactc attttgttca atccctgact
    20761 tgagtaagaa aggatcattt caggtgtttg aaaagactgg cctgcattca attcccacga
    20821 aaaacgatgg ctgttgatcc caccgacgac tctcaattga ttgatctgat ctttttcaag
    20881 ttcgaaagaa tggttgccgg aataaacaaa ttgaagtccg atcgcttctc cagtattttc
    20941 tgtcgtatga tgatgaacta aagcaataaa gttattcata tggtgactgc tcgttcctcg
    21001 acggctttca aatacttttg ttccagataa aacagattgc cgtgaaatct gtctttcatt
    21061 gacatgtgct cctggcaaag caatgacttc atcaaatcgc ctagtatggg caaagtctag
    21121 ctggaaacta gccgcttttt ctagtttgat tgtttctccg gtttcatttc ttatctttac
    21181 agatcgtgtg atcactgctc tatcagtaaa tatggtatag aacaaatcga aataaatcgc
    21241 tgtttctcta tccttaagtg aaatcaccaa cgtttgtgcc tctgacttgt ccatcacata
    21301 agtttgaggc aagccttcga tctctggctt cccatctgta atataatggc tgtcatatct
    21361 cagatccagt aagtctgatc cattcttgcg ctgaatgatc gatgcgggcg ttcgaaagtc
    21421 catgctgcca tgagaagagt attcttgcgg tagtgtatct ttcgacagag accggtcagg
    21481 atttaaggga acatttcctg aaaatccacg atctcttcgg ggataatttt tattgtcttt
    21541 gtactgcttg actggtttgc caaaataaat atgcgccaat acattgcttt cttctatctt
    21601 tatcagataa gaaagaaatg tatttcgtaa atggaagact cggctctttt catcaaataa
    21661 gatgaaattt ttatctgttt ttctatgcgt aatatccatc atttcactcc caacaaaacg
    21721 gaaaccgttt actttttttt ataattcctg tatactgttt actaaacaat tttgtcaata
    21781 aaaatttttt atttttttga ggagtgtgta aaaatggttg gaattagaga tgtcgcacga
    21841 aaggcaaatg tctctcctgc tacagtttcc cgtgtgttga ataacgatca gacgatccat
    21901 gtggctcaga ctactcggca acggatagta gaggcagctg ctgcgttgga ttatgacatc
    21961 agtactcgta aatatatcaa aaagagaatg ccttcgattg gtgtgatctc tacgatcggc
    22021 aaagcgagcg aagaacagga tatctattac aaacatttgc gagtagggat ggaagaagaa
    22081 gctcgacgtc tgcatttagg gatgaatcga atctataatc tatctgataa tcctaaacaa
    22141 tgggaagatc tagatcagtt aggtgcggtg atcgttgttg ggactgttac gaaagaatcg
    22201 attaccgcct tgttaaaaca aaacatgcaa atagtcgtcg tggataaccc tgacatccag
    22261 caagaggtcg atatggtgta tgcagatctt gaacggatga ctaaaaccgt actggaattg
    22321 ttcttagaaa atggtcataa aagaatagcc tatatcgggg gatataaagt cgatatggat
    22381 gaattcggtg ggaagcagat ttctataaat gaaaagagga tgcgtgccta tcagcatttt
    22441 atgatcgatc atcacttaga aaaatatatg tcttatcatc taggtggctg gacagaaaaa
    22501 caaggtgagg tacttgcgac taatctatta gaaaatgaat cccctcttcc tactgccatt
    22561 cttgtcggga gcgatccaat ggcaattggt gtctatcggg cattgaaaca aaaaaatatc
    22621 caaattggag aagagattgt tgtagcaagc tttgataata tcgagacaat cgcaaacctc
    22681 tcccctggct tgaccagtgt tcaaatcaat gcgaaatcaa ttgggcaagc agctgttcga
    22741 atggcattgg agcgaattga cggtatacgg gacgaaccaa tcattttgac ttatcctgca
    22801 cgattgattg ttagggaaag ttttatccca agaacaaaaa ggtattgaaa gagaggttgt
    22861 gaaaagccgc ttcgtttctt catactcaaa acgaaacggc tttttcacaa cctcttactt
    22921 atacttattc acaaaatcca atgtctgctg gtaacaattt tgcgattctt ttagcgtgta
    22981 atcaaattgg tattcatggg taatctcttt ttttgtatct gtataaaaca aggattgcac
    23041 gggtaccttt aattctgtca accgattttc taatgcttga ccttgctcct ggaatgagta
    23101 tgcatttccg tcagttatat acgttggcgg aaattcctta gaaacatggt cagccacact
    23161 agcttctttc aattgctcac tcgttttcca atcttttgtt ccaatcaacg accacgcaac
    23221 ggttttgacg aaaaacttca taaatcgatc atccgaatgc tgctgtgctg ttagcttcaa
    23281 atcaacaggt ccgcaataag aaatggttcc tttcagacta tcttttggaa gtagctgttt
    23341 gattttcatt tgtttagcat acgcatcatt cgtttgtata gctgcatatt gcaatgcgat
    23401 ttgtgaacct gcactatctc caccaaaaaa taattgttcc atgtctagca atttgtcgtc
    23461 tttttcttca gctaataaat ctttgatcag ttcattcact tgaagaactt gattagggta
    23521 ttctgaatca ggcgctaatt cataattcat cgcaaccact gcgatgttgg cgtctgaaac
    23581 taattttgtc gcaaattctt tgacacccga tttatctccg ccgacgaatc ctccaccatg
    23641 tacccaaaat agaacaggaa caggcccctt gctgtttttt ggatagtaga tgtcataagt
    23701 attctcttta tacttggact catacgtcaa atctgttttt ttcgccacgt ttttttctgc
    23761 ttgatcgaac gtttttttat cagtgatttg aacagtattt gaaaacatat ggttgatcaa
    23821 gaatgctcca ggtctggggg aaagctggaa ggcaagaaag attgcaaacg cgatcccaag
    23881 aatgattccg aagaaccatc cgataaattt aagaattttc tttttcataa ggctccctca
    23941 gtagttagta ttaagcattc tgattatacc atagtgttat ttcctatcaa gctctcctag
    24001 atgatttaat gattttcccc aaacttcttc tgcaatgctt ttgacaaaag aaattttttc
    24061 cattgttctt caaacaaaac attttcttct tctgttgaag caaatccaca ttgaggactt
    24121 aagcacaaac gatccaaagg cagatatcgt gtcgcttcgt agatccggtc aatcacttgc
    24181 tggtgctctt ccatttcgcc atttttcgtt acagtcaaaa tctaagacaa ctaatttatc
    24241 ttcagagact ttctctaaaa gaacaaaact cccagcacgt tcactatcgt attctaaata
    24301 ataggcacgg acattttctt gagtaaaata atgggtctgc tacgtcgtca tcccccctga
    24361 tgccgccaaa atcgaataat aatttccgcg tcaaacatgt atatttagtg ttgaatcatt
    24421 tgagaatatg aaaataaaaa tctttttgat caatcagtta atcaaattga aataacgaaa
    24481 aatctgagac agaatgacgt tcattctgac ccagatttga ttttgcacaa ctcaacagtc
    24541 cttattggca aatcgccaaa ataatgtaga ctaa
//
LOCUS       DACECR010000039        24371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.039, whole genome shotgun sequence.
ACCESSION   DACECR010000039 DACECR010000000
VERSION     DACECR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.039"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(100..414)
                     /locus_tag="IWO17_001415"
     CDS             complement(100..414)
                     /locus_tag="IWO17_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ0290299.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(428..688)
                     /locus_tag="IWO17_001416"
     CDS             complement(428..688)
                     /locus_tag="IWO17_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ0290300.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            858..1172
                     /locus_tag="IWO17_001417"
     CDS             858..1172
                     /locus_tag="IWO17_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0290301.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            1174..2829
                     /locus_tag="IWO17_001418"
     CDS             1174..2829
                     /locus_tag="IWO17_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0290302.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            2912..5047
                     /locus_tag="IWO17_001419"
     CDS             2912..5047
                     /locus_tag="IWO17_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0290303.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            5222..5731
                     /locus_tag="IWO17_001420"
     CDS             5222..5731
                     /locus_tag="IWO17_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290304.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            5728..7032
                     /locus_tag="IWO17_001421"
     CDS             5728..7032
                     /locus_tag="IWO17_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0290305.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            7128..7997
                     /locus_tag="IWO17_001422"
     CDS             7128..7997
                     /locus_tag="IWO17_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0290306.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(8109..8429)
                     /locus_tag="IWO17_001423"
     CDS             complement(8109..8429)
                     /locus_tag="IWO17_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ0290307.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            8583..8978
                     /gene="dhaM"
                     /locus_tag="IWO17_001424"
     CDS             8583..8978
                     /gene="dhaM"
                     /locus_tag="IWO17_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ0290308.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            8980..9963
                     /gene="dhaK"
                     /locus_tag="IWO17_001425"
     CDS             8980..9963
                     /gene="dhaK"
                     /locus_tag="IWO17_001425"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ0290309.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            9991..10596
                     /gene="dhaL"
                     /locus_tag="IWO17_001426"
     CDS             9991..10596
                     /gene="dhaL"
                     /locus_tag="IWO17_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ0290310.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            10743..11408
                     /locus_tag="IWO17_001427"
     CDS             10743..11408
                     /locus_tag="IWO17_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290311.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            11691..12350
                     /locus_tag="IWO17_001428"
     CDS             11691..12350
                     /locus_tag="IWO17_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0290312.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12427..14037)
                     /locus_tag="IWO17_001429"
     CDS             complement(12427..14037)
                     /locus_tag="IWO17_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ0290313.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(14050..14931)
                     /locus_tag="IWO17_001430"
     CDS             complement(14050..14931)
                     /locus_tag="IWO17_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0290314.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            15185..15784
                     /locus_tag="IWO17_001431"
     CDS             15185..15784
                     /locus_tag="IWO17_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0290315.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            complement(15853..17153)
                     /locus_tag="IWO17_001432"
                     /pseudo
     CDS             complement(15853..17153)
                     /locus_tag="IWO17_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            17733..18989
                     /locus_tag="IWO17_001433"
     CDS             17733..18989
                     /locus_tag="IWO17_001433"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ0290316.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVGALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            19280..21157
                     /gene="tdc"
                     /locus_tag="IWO17_001434"
     CDS             19280..21157
                     /gene="tdc"
                     /locus_tag="IWO17_001434"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0290317.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            21400..22821
                     /locus_tag="IWO17_001435"
     CDS             21400..22821
                     /locus_tag="IWO17_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0290318.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            22930..24297
                     /gene="nhaC"
                     /locus_tag="IWO17_001436"
     CDS             22930..24297
                     /gene="nhaC"
                     /locus_tag="IWO17_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0290319.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT         7994 a         4397 c         5037 g         6943 t
ORIGIN      
        1 ttttcacaac ctttgattca cggttttcca gaagtagctt cttcgaaaaa taagccgaaa
       61 tttcttgaag ctgatcactt ctgtcgcaac ctctactgac taaggcttga tataagcata
      121 accttgggat tgcagttcaa ctaaatcaag gacacctgct ggtacaacga ttacttgttc
      181 aggtaaagaa ctttctgaaa tatgattagc acgcatagca ttggcgcaag cgtgaaaaac
      241 gactccttgc aaatctaaga attctgcatt ggcagagtct agataaccgg taatcgcgat
      301 gccatttaca gatacgacaa tcgtcgtttc tggagaagct tttatcaggt tttttacgtt
      361 tttccctgtt tctgaccatt tttctaattc atcaatatgg aaaacgactt tcattgaaat
      421 cattccttta ctttttaata atcatcttga tcgcttgttc tagtttagcc gcttgttctt
      481 ctggattact atcaggtttc tcaaagcaat ttttcagatt atctgcaacg atcatgccca
      541 ttacgcgatc aatactagaa cgaacagcgc ttaattgagt gatgacatcc atacattctt
      601 tttcgtcttc gatcattttt tggataccac gaatttgtcc ctctgtacgt ttcaaacggt
      661 tcaggatatt tttattatca atctccataa cacattctcc tctattttta taattagata
      721 atacccctta tcggtatttt atgcaaatat aaaaagtagt gaaaaaaatg aagagtcatt
      781 ttggttgaca tattaataaa gctcctgttt aatatacccc atgaggtatt tataaaaatg
      841 taaatgaggt gcagaaaatg aatttgctat ttcaatcaat aaaatcgata acagtcccag
      901 aattaaagga aaagcttttg gaaaatccag cgttacttga tgttagaaca cctgcagaat
      961 accggggcgg gcatataaaa ggagctaaaa atgttccatt acaaagtata aacagatatg
     1021 atggagacaa agaaaagacc gtctatgtga tttgtcaatc agggatgagg agtaaacaag
     1081 cagctaaaga attaaagaaa tctggttatg atgtagtgaa tgtacgtggt ggcatgaatc
     1141 aatggtttga tcgaacagta ggagggaaat aagatgaaaa tcgttattgt cggaggtgta
     1201 gcaggtggaa tgtctgctgc tacacggctt cgccgattaa tggaagatgc agaaatcgtt
     1261 gtttttgaaa aaggaccgta tgtttctttt gcaaattgcg gcttgcctta ttatctttcg
     1321 ggagaaatca gtgaacggga aaatcttctt gtccaaacgc cagaatcatt atccgctcgt
     1381 ttttgtttag atgtgcgtcc aaatcatgaa gtgacagcca tctttcccga aaacaaaacg
     1441 gtagaagtcg tacatgaggg tcaaaaacac attgaacagt acgatgcatt ggttttatct
     1501 cctggtgcaa aaccagttgt tccatcgatt ccagggataa cagaagccga caatgttttt
     1561 tctattagaa atgtaccaga tatcgataaa gtgatgcatg cattagaaaa acagccaaag
     1621 cgtgccgtga tcgttggtgc aggattcatc ggattggaaa tggcagaaaa cctaaaaaga
     1681 agaggtttag aagtcatggt gatcgaacaa gcaccacata ttcttccaac actggatgaa
     1741 gaaatggcag cttttataga aaaagaattg tctcatcaag gagtagaagt gattacttct
     1801 catgctgtcg ctggatttga agaccacggg aaacgattgc gactggatga tgggcgtacc
     1861 atccctgctg atttagttat tttatccatt ggtgttcgtc ctgataacca gctagcagtg
     1921 accgctggaa tcgaattagg catacgcggg ggtatcctag tagacgaacg ttatcaaacg
     1981 aatattcctg atatttatgc ggtgggggat gctatcgttg taaaacagca aatcactgga
     2041 aaagatgcac ttatttctct tgcttcacca gccaatcgtc aaggtagaca agttgcggac
     2101 acgatttccg gaatttctcg aagaaatcaa ggcggtattg gaacagcaat tatacgaacg
     2161 tttggaatga ctgccgcatc caccggttta agtgaaagaa cagccaaaga aaacgaactg
     2221 tcttttgaag tcattcatgt atcaggaaaa gatcatgcaa gctattatcc agaagcaaca
     2281 gatattttac tgaagttgat cttccatcca gagactggcg agatttatgg tgcacaaggt
     2341 gttggggcaa aaggtgtgga taaacggatc gatattttag caacagcaat caaagggcat
     2401 ttgacgatct tcgatttacc ggaattagaa ttgacgtatg caccgccatt tggctcagcc
     2461 aaagatccag taaacatgct aggatatgca gcaatgaaca ttgcagaagg gcttagtgaa
     2521 accgtacaat ggcatgaatt gccgacagaa ctagcaaaag gaaaaatttt attagatgtg
     2581 cgaacagcag aagaattgga aaagggcaaa ttcaaggaag ccaaacatat ccctttgaat
     2641 gaacttcggg accgattaaa tgaattagac agccaacaag aatatatcgt cagctgtcat
     2701 agcggattac gtagctatct agcggaaaga atcttgaagc agtctggcta ccacgtaaaa
     2761 aaccttgatg gtgcattttc tttatatcaa actgtccgac ctgaagaact gatatatcct
     2821 aacaaatgat actcgttaaa aagcttgcat tcgactctta aaagaggtaa actaatcggt
     2881 tccaccactt taaaaaaaga ggagaatgtg tatggtcaac acacaaaaac agttagaaga
     2941 agcatatctg aagaacgtgt atcaaaagct aattgataaa aaaaacgaac tgcgggaatc
     3001 gatggatacg gcaaaaatag aaggaaacca gtcgttgaaa gaaatggctg gcgagcttcg
     3061 attgaatttt gacagttact tagataacct ggatactttt tcgatgattg aaatgaaaaa
     3121 tagagaaatc gatcagatga atatcaaaca gaaaaacgca gaaaatcagc tagcaaagat
     3181 cgaacgttta ttgaaaagtc cctactttgg aaaagtcgtt gtagactttc tggataatga
     3241 gccaaaagaa tccttttata tcggtacagc aaatttcaca gatgatgcag atgaaaatct
     3301 tgtttatgat tggcgttcac caattgccga acttttttac aataacatga ttggtccatc
     3361 ttcttatacg gttcgtcaaa ataaaatcga tacggttatt aaagaaagac gacaatttct
     3421 tttggaaaga gatcgtttgg tcaagttttt tgatacgtct gtagctattc aagatgacgt
     3481 attattagaa gtcttaggcc aagatgagac caatgaaatg aaggctatca ctgctacgat
     3541 ccaatcggaa caaaacacaa tcattcggga tgtgaaaagt caaaatatct tggtaaatgg
     3601 cgtagcagga agtggtaaaa cctctgctat catgcagcgt attgcgtatc tgctctattt
     3661 atatcgagat acgatgacat ccgatgactt gctgattcta tcacctaatc atcggtttat
     3721 cgactatatt tcgaatgtcc taccatcatt aggtgaaaga aatccactga atctaaccat
     3781 catacagctg gtttcacagc tttcagcaga ggaaatagaa ggtgaagagg cttatttcaa
     3841 acgtatcacg ggagaaaatg tatcggagca gacagaacgt ttgcgtagca aaaagtttat
     3901 cgataatcta aaacaatcag atcctttgtt tttagatcat ccgaatttta tccgtggact
     3961 tactaaaaat ggaaaaacag tcctttctaa aaaaacgatc gaaaaaatat atgagaaagt
     4021 tcctgctcat cctaaattga tcgataggct tcaagcaacg aaaaaggcac tgatgagcga
     4081 atggaagaat catcttttga aacaagcaaa aagtcctgct gtgcagaatc aggtactctc
     4141 gttgacagaa gataggcagt tggaattatt cggtaaattg atttctgatg attcggaaca
     4201 aagtattgta gcttatgcaa ggaaactttt acaaaaaaaa tatcgaaaaa taaccagaca
     4261 aatcgaagaa atggcatggg tggatgagca tcagttgttt gaaagaattt atgaaaaacg
     4321 atatggctca gcttatgctt ggcaacccac acgtactgtt gatgaagcag tcattatatt
     4381 agctattcgt catttacttg ttgaaaaggt aaatgtacca gcatttcgct atctattgat
     4441 tgatgaagta caagattata ccctggcaca attaggtcta ttgatcgaat tatttccaaa
     4501 aactcacttt actttagtag gtgacgagaa tcaggcaatc tttaattctt ccactacttt
     4561 tgctgatatt atgcgttgtt tcgacgacta ccatctgccg atccatcggt atgatcttag
     4621 aaatagttac cgttctagcg gagcaatcac agagcttttc aaaacctatg ctgtggatca
     4681 agagaagatc gacatcgtat cgatccgacc gcaaggaaaa gaacccgaat accgctcttt
     4741 tcgatcctct gaagaattgt tgaaaatact agctgagatt ttggtttctc taaaaggaga
     4801 aaaagcggca gttatcaccc aaacagaaga ggaaacccaa acgctacaag aatcgattaa
     4861 aaaaaattca tatggattag aaaactgcca aatcattcct ttgagtctag caaaaggact
     4921 ggaatttgat catgtaatct tatatccatt tgaaaacgat ggagatgagc aaagaagacg
     4981 cagacaaatg tacacggcga tatctagagg aatgaaaagc attgtcgtat tggaaagagc
     5041 aacataatta agagataaaa aataaaaaga acaactgata aaaagaacaa aatatttttc
     5101 atttgctgat gagaaatgtt ttgttctttt tcttaggaaa aatttaggaa cactttatgt
     5161 gaaaagaaat gaaatgtatg gtaaactcct acgtcgtaac aaaaaaacgt gaggtgagga
     5221 tatgaaaaaa gtaacaagag aagaagttgc atcaattttg gtgaaagata agtatttttc
     5281 aaaaggtaat tactggttaa agatttggca aacgttagtg gctcttttag gatggatgat
     5341 cgttgttctt ccttttatat gggtcttttt cccattaacg agaccagaaa gagccaagga
     5401 tttttccttg tacgcttttt tatcagaaaa gaaaatgctg tatttcttaa tcggcttttt
     5461 tgtattgatt tttttcagtt tcctgattac ctcatttgta ttgactaggt ggaacaatcg
     5521 aaatctatac aaaaaagtaa ataaatcttt tgaatatgaa gatgaaaaat tgaaaaaaag
     5581 acaagaatta cttgaagaag tgtatacgga acgttttgga acaaaagaag agagagaaac
     5641 ggtccgtttt tattctgtct ccgaagagaa aaatctagat acgacattca ttgcagattt
     5701 gtataaagaa aatggggtgg aactgaaatg atctcaatca cccatacagg taatttattc
     5761 ttagatactt gtttgtctat tatgtatttt tttctcgttt cttatccgat tttaggcgga
     5821 tttgtatggt ttattggtgt ctggtgttat gtgtttttat acaaacataa gcaaaaagaa
     5881 tgggtagatg ttcccttgag tgtcgaaccg ttcatcacga tcatggtgcc agcccataat
     5941 gaagaaattg tgatcgaata tacaatcgag tatttgatga cgaaattgaa ttatcacaac
     6001 tatgaggtat tagtgacaga cgatggttca acggaccaaa caccagaaat attagctaga
     6061 ctgatgaaaa aatacgctaa tcttcgtgtg gttcggatcg aaaaaaataa aggaaaagcc
     6121 cacgcgttta atatcggact tgcctttgca aaagggaaat tgattttaag caatgatgca
     6181 gacacggtac cggagccaga tgcactgatt aggtatgtga attatttcat tcgccctggt
     6241 gcaagacata ttgctgcggt cactgcaaat atggatgtcc aaaatcgtac caaattgatt
     6301 gcaaaatctc aaacggttga attctcaagt atcgtgggca tcattaaacg aacacagtca
     6361 gctgttttcg gtggtcttta tgcgtacagt ggagcaaata cgatgtatcg aaaagaagcc
     6421 ttgatcgatg tgggcggatt cagacaagat cgagcgacag aagacatcag tattgcttgg
     6481 gatcatcagt taaatgactg gttatctgtt tttgctcctg agattatctt tttcatggaa
     6541 gtacctgtga cgttgaagat gctatatcga cacagaaaac gttgggcaaa aggtggaaca
     6601 gaagtctggc tgacgaattt caaaaaagtg atgctccatc cttttaaaca tattggaaga
     6661 acgatcatct tcattgatca aacattgagt attgtctggt caatattctt ttgtatttcc
     6721 gtagttcttt ttgctggatt aatcgggcat tatgtatatc aaggaaacta cgaacagatt
     6781 tatattactt ttacattcag ttttgtgttc atctgctttg aaatggtcgc aggatttttc
     6841 cagttgctag cttcgctgat tgtagatgat cgacgaagaa aattgaaata ccttcttttc
     6901 gcaccactat atatgctttt gttctggata gtgaacgcga taacgatcgt gacgactttc
     6961 attccagcag tcaaaacgat tttaggttat ggaagcggga catggaaaag cccggaacgt
     7021 acaaaaaaat aaaagatgca ccgtttttta gacaaacagc ctgttgtgtt tatggttctt
     7081 ccaaacattt ttgcttataa taagaaatta gttaaaaagt agtaaggatg ggaaaagtga
     7141 tgaaaaaaag aatgaagccg ttaatctggc caattgctgt ttccagtatt ttcttattgg
     7201 gacagacagc ttttgcggaa acttctgaac ctgctcaaac agaaccttca acaaccataa
     7261 gctcttctgg tgctgaaaac ggtgcctcgg aaacagaaat gacttccaat gtaccgacag
     7321 ataattcagg agaaacgatt tcatcttcag aagatacatc aataaatagt tctacctctt
     7381 ctgaccaaat cgatcatttg caagacttgg aaaataaaag aaaagaactc gaagaagcat
     7441 tagaagctgc aaaaaacaaa ttgaaagaag accaagcagc aatcgatcaa gatttagctg
     7501 atcttaaaaa taaagtggga aatgaccgtg cacaacttga gaaaaagctt gccgaagcga
     7561 aagcaaaagc ggaagctgtc caaacagatc ttgacgggaa gatccaacaa gcaaaagaat
     7621 tcaatcagca attaaatcag gttgctcaaa cagcttctgg aaagctaagt caaactcaag
     7681 cggatatcac agcggccagt caagcaatcc acggaaagat cagcgaagca caaacagttg
     7741 ctaatgattt tgaagcaaaa aaagctgact tagcagctag tttccaacga ataaaagata
     7801 ttttgcattc ggcaggagaa aaatcagtga cctctggaag cacgacagaa acagttcagc
     7861 aaacgacaga gccatccaaa tcaacaagtc agactttgcc aaaaacaaat gaaaaaacag
     7921 cttctgtcag ttattcctta gcaggggcgg gtgtgttagt cgcagcagct tacggaatca
     7981 tccgcaaaaa gaaataaaaa agagaggatg ggaaggtaga gtacctcgaa gttagactga
     8041 aaaatctaat tcttgagtgc cactacatgt cccatctctc tttagttatt aaaacagatg
     8101 tcccctactt atctcgtgga gacagctgtt ccactacatg tgaccatcag catgccattg
     8161 tcgctgccta atacttcata gtccattttt acgccaatga tcgcatcagc tcctaatgct
     8221 tttgcacgtt cggccatttc ggcaagtgct tcttctcttg cacccattag ttcgttttca
     8281 tagctttttg agcggccgcc aaagatattt cgcaaccccg cctccaagtc tttaaatgca
     8341 tggatcccag taatgacctc accaaaaacg atccctttat aagctactac ggcttttccc
     8401 tctacactgt tcgttgttgt tgtaatcatt cgatcactcc tctcttttaa ttaaagtata
     8461 acaaacttca agaagcacta cacatatttt cccaaaatta gaaatttatt atatacttga
     8521 attgttcatt aatgaaagcg aaaccaatcg ttagcaacta tgtaaaagaa aggaagattc
     8581 ttgtgaaaaa aagtattttg cttatttctc atagtcaaaa agtaacagat ggtattaaag
     8641 aaatgattga gcagatgcaa aaatccgaag aggtgcaaat tttttctctc ggcggaataa
     8701 atggcgagat cggatcagac ccgatgaaaa tcgtagaagc agtcaatgaa tcaagcgatg
     8761 cagctagtta ttttgttttt gcggatatcg gtagcgcggt attgaactcg gaactggcaa
     8821 aagacatgtt ggaagaagag cagcagcaac gttatcatct ggtggatgcg cctttagtgg
     8881 aaggagcttt tgcagcagcc atcactgccg gtataacaga tgatactcag caaatcttaa
     8941 cagaagcaca aaatgccgga aagaaaggat gggaataaat tgaaaaaaat catgaatgat
     9001 ccaagcaata ttgtcgaaga aatgctggaa ggattagtaa aaagctatcc agaacttgtt
     9061 catcgtgtag aatcttcccg tgttgtagcc aagaatcaga aagcggaaca agttggtttg
     9121 gtttctggtg gaggaagcgg acatgaacca tcccatgccg ggtttgtagg ggaagggatg
     9181 ttaagtgcag cggtgcttgg tgatgtgttt acttctccaa ctccagatca gattcagacg
     9241 gctattaagg aagccgatag tggaaaagga gttttgctga tcgttaagaa ttatacaggg
     9301 gatgcattga acttcgatat ggcaaaggaa ttagctgcta tggacgatat tgaagtagaa
     9361 agtgtgatcg tagatgatga tatcgccgtt gaaaatagta cgtatactgc tggaaaacgc
     9421 ggagttgcag gtactgtcct tgtacacaaa atcgtgggcg atgcagcaag aaacggtgct
     9481 tccctagctg agttgaaaga actgggagaa aaggtcgtac aggcaactaa gacgatcggt
     9541 ctagcgcttc gagcagcaac tgtaccagaa gtagggaaac caggattcga actaggagaa
     9601 gatgagatcg agtatggtgt tggtatccac ggtgagccag gctatcgtcg agaaaagatg
     9661 cagccttcta aagtactggc aaaggaactt gttactaaga ttttagatga ttactcgaat
     9721 ttaccaaaag aagcaggtgt actagtcaac ggaatgggcg ggacgccttt gatggaacag
     9781 ttcgtgttca tgaatgatgt tttagctctg ttggaagaaa gaggcgtcaa tgtagttttc
     9841 cgaaaagtcg gtaattttat gacctcacta gatatgcaag gtctttctct aacactgatc
     9901 gacttgacgg agacacaatg gaaagattct ttagaaagca atgtacagac gatttcatgg
     9961 taaaaaatct caagataaaa gggtgataat atgaaactaa ccgttcaaga aatccaagaa
    10021 tggctcacgc aatttgcaga ggcaatcaat cagaacaaac aatatctcag tgatctagat
    10081 accccgattg gcgatggaga ccatggaaat aacatgggaa gaggggtttc agcttatgaa
    10141 gaagcttttc aagccgatca tcctgaaaca atcagcgata cattcaaagt attctctatg
    10201 gcgatgatat cgaaagtcgg tggggcttct ggtccgttat acggatctgc attcatgaac
    10261 atgatgaaag cgacaaaaga ggtcgattct attgattctc aagaaaaatt aggcgaagtg
    10321 atcgagcagg gaacagcggg tattcaaagc cgaggaaagg cagaagctga cgataaaacg
    10381 atgttagatg tttggctgcc agtcacagag gcattgaagg cagggaattt aacaaaagaa
    10441 atcattgacc aggcaaaaga acacaccaaa gtcttggtag ctaaaaaagg acgagcttcc
    10501 tatctaggag agcgtgcaat cggacatatc gacccaggag ctgcttctag tgccatttta
    10561 ttttcaacgc ttttagatgt gatcaatcaa ccttaagttc aataaaactg cggtgatgct
    10621 catcgcagtt ttttgtttgg ctgttcttat gacttgatgc agaacagctt tttcacaacc
    10681 tctatttgtc catatcgcaa aattcaggta gaatacaaaa gataacggaa agaggcgaaa
    10741 caatggaact tggaaaatca gattatcagt tgtttgatcg accaatctac gcattcaaac
    10801 agctcaaaga gagccatcca acagataaga ttgaacaaat caagaaagaa tataaagaac
    10861 actggcaaaa atggaaagag attcagctcc agacagctgc acttttgcct gatatgtacg
    10921 gcatgagcaa acctaaaatc gaaagctgga cgaatggctg gaatttacgc agtcattttt
    10981 ggagcgcgta ccgaagcgaa tctcgtcaag acgagaatgc ctgtctagca gttttgttga
    11041 atcaaaaaca ataccaaatc tatttgatgt accaacatta taaaagcgaa gaacgatatg
    11101 gtagcattgc agcgtataac cagttgcttg cgatactgaa agagtggagc aaaacagtcg
    11161 atatcaagga ttattacata tggccgcaac cagaacatga acttgacgac cacttagctt
    11221 tgtctgacta tttgtccgat acaaagaaac aagaagagtt gaaaaaagcg atgagagacc
    11281 gaacctttca aatgggtaaa ctattcttct atccgcaaga aatcgaacac gttgagcaaa
    11341 taaccgctga aaccttacaa gaactagcac cactatatca aaagctaggt gcggaaaaat
    11401 ttcaatagac aacacgaaat aagcaatgcg aaaaacaaac cttactctcg gcttgcgagt
    11461 gaaggtttgt ttttgattat tcaaaaagta aatatttaaa aaattgatag gaatcaatta
    11521 cttaaggtct caaaagaagg aaaataaagc aagggacaaa aagaaatcca ctctatttgc
    11581 tggtatctgg ttcgcttgac gtttatgcta tgataaaaga aaattatcag ttggaataag
    11641 ggaagttatg tttctttatt agaaatagct gtttagaagg aggagagatt atgagtcatg
    11701 atcatttatg tgtttcattt gttcctttgt tcaatcactt agtacagcaa gatcaagaaa
    11761 aagtacacgc acttgtcaaa catcactatg tggaaaaagg agaacagatt cttgcgccga
    11821 atggagaacc tcaattagtg atcgtggcct ccggcagtat gaaagtgtat caattatctg
    11881 ctagcggaaa ggaacagctt ttacgagtaa tagagccggg cggatatgag ggagagagcc
    11941 agttgctagg cgttccaaat gagtctttat ttggcgaagc actagaaaaa acggaaattt
    12001 gcatattgag acaaaaagat ttctcggaac tcttactgca ttatcccgcc cttagtctga
    12061 aacttttagc aatcaacgtt gagaaattaa caaaagtaca gcagcagaca acttttttga
    12121 tgatggaaaa agtagaagaa cgagtcgctg tctatctgct tgacttagct aaagcgacag
    12181 ggaaggatca ctttttcctg ccaatgaaaa tgaaagaact tgctgcattt atcggtacga
    12241 cccccgaaac tttatctaga aaatttaaat tattagagga gaatggttat gtaaaaagaa
    12301 agaatcgaga agtctggatc attgataaag agcagttaga agacttgtaa ataaaaaaat
    12361 gacctccaaa tgttggattt ttgacccaga tttggaggct agcatgaaat tgtacatgct
    12421 tttttattat ccttctagta agtctcttcg cctataacca atagaaccaa ggacgaacag
    12481 tgcaaggctg atacatgata acgtaaggaa cacggaaaga tcaaaagaat cgtttggcat
    12541 ccgtggaacc cagctaggga cagctgtttt ggaaaaccat tctggtaaat ccaaaattcc
    12601 gccaaagtaa ttgagtgcaa aggagtaagc taagtagata taagcaagtt taccgattcg
    12661 aggaagccat ccgaatagaa agccactaat tccgataaac actaaaacgg caggcaggaa
    12721 attgaacccg gctttaagaa acatcaatag atccatgtct ttcgcatcct tcatcgaagc
    12781 caaagcagaa agccctagtg caccagaact aatcaggatt gccaatatag caaatagaat
    12841 agctagtaac atattggtcc aataaagttg gttcctagaa atttttgttg cgaacagctg
    12901 attcatacgt ccgtgggttt cttctgtaaa taaacgattg atgctagcaa ttggtaaaat
    12961 agcagctaga caaatcaaga cgaccatgat cgtgcttgta aagttttttt caattgagac
    13021 gccagattgg ataaacatct gtttcattaa gtcgttactt tctaaaaact gttgcatatt
    13081 cccgtagata gagccgtatg ccgcccccat caaaccaaaa gtgataagcc aactgattgt
    13141 catcccttta tttagataga ataataaacc acgcaaggat aaaagagaac gtttagcata
    13201 cggttttcct tcacgttcgg ggatatagcc ggtgttcaaa tctcgatgtt cctctaaaac
    13261 aaatgaactg attatcaaga ttagactgaa caaaaacaaa taaagcaaag gaatccagtt
    13321 gttagttgta aatggatagg tcaaataaga ccagcctaaa ggattccaca tcgaaaggga
    13381 ggaatcagct acatctgtta ttgctcgtag aaggtaaagt ccacctaaaa acgctaaaga
    13441 cagaccattc gaactggctg aacttggcat cagttgtgca gcaaacagtg caacggcact
    13501 ccccaaaatc cccatactac ctacagatag cccaaaaagc caagcacctt ctgcagtgat
    13561 cgaatccgct tggaaaaaaa tcatcgtaca actgatcgat attgctaaaa gcaaattgat
    13621 cagaatactt tcagcgatga cagctaaaga gtttgcttga cgtccgactc gaaaggaacg
    13681 aataagttca atcagaccaa gttcttcttt ttttctcgtt tggctcacca cgtgtaaaac
    13741 agagatcaca gcagcaaaca aactgcaaaa aagcaacatt tcatttgcat acatcgctcc
    13801 taaagtatac tcagcagcat tcgaagctgg agttggtccg accattgcaa tcattgccgg
    13861 gttttgcaag gtttcgtata atccagctaa tccttgtccg gaagtcaatt cttcaaacgc
    13921 agggacaaaa ccaccagaaa aagcagtgac accgactagc catatcagga tctttttcca
    13981 gtcacggcta aggttttgag aaaaaagggt ggaccagcgt gaaaatttat cagtcatttc
    14041 gcttcctcct catccttcat aatgtctcat gaataaatcc tcaagagtcg gtggaataga
    14101 ttcaaatttt ttcacaccta atttggacgc ttcagctaaa atcacatcga aagcttcgtt
    14161 atcagcagca aattgagcga ggtgaccttt ttgcacaaag tcatggacac cgttcaattg
    14221 tgctaagtga ctattctctg cttctgtttc tatagtgatg gtcgaacgag tcaaatgacg
    14281 caattcatcg agtgttcctt cttcatcgag tgttccttcg cgtatgatca cgactttatc
    14341 cgctagtcgc tcgacttcac tgagaatatg agaagaaagt aggatggatt tgccctgttc
    14401 tttcaatttt tcaacttctt cttggaaaat cgtttccatc aaaggatcta aaccagaagt
    14461 aggctcatca aataaataga gatccgaatc aacagccaaa gcagcaatca agcccacttt
    14521 ttggcgattt ccttttgaat agttttttgc ttttttctta ggatcaagag agaaacgttg
    14581 aatcagttca tctttttttt gagcactccc ttttccatga agcttcatga agagatcgat
    14641 gatcgctcct ccagtcaaat ttccccacaa agagatatcg cctggaacat aagaaagacg
    14701 ttcatggata gataaactat cttcccatac atcttgccca aaaatcgttg ccgttccgcc
    14761 gtcttttttt ataatcccta aaagtgtccg aatcgtcgtg gattttccag aaccatttgg
    14821 tccgataaat ccaacgactt cacctgaatg tacggtgaaa gagacatctt ttaaagcttc
    14881 gaaacgacca aagctctttt tcaaatgtgt gactttgacg atttctgcca taaaaacccc
    14941 actccttttt ctaaaaatat atactaaaca agattaaata gtttaataaa aaggttttat
    15001 acctttttat aaaacttttt ttattatttt agtacataat attcttttta taaatttctg
    15061 tcaatgaatt cataaacaag gaattgctag cttgatactt tctcaataga tagttataat
    15121 aaacctcaga ataaatttga actatatata ttataaaagt ttaaaattac gggaggctct
    15181 acaaatgaac ggttttgaaa agagagcaca agagaaaaag aaagagattt tacaagcagc
    15241 ttttgacttg atgaacacag acataggaac cgctggagtg acaatggata aggtagctga
    15301 gaacgctcag gtaagtaaag cgacgatttt taaatatttt ggcagcaagg aagggctgat
    15361 ttatgaagta ttttttgcat ttatgaatga tttagggcaa gaagcgaaag cgttgattgc
    15421 ggaaaatctt ccatttgaag aaacactgat tgccatgagt gaaaacaaaa tccgttatct
    15481 caatcgagtg aacaaacaat tttatatcga tttgatgaca tactatacaa aaaaagaaaa
    15541 aaaagaattt tctgacgcga tggatgcgta tacgcaagaa agtttcagta tcatgcttga
    15601 tcttttccat cgagggcgga aagaaggaaa agtggatttg aaatattcgg atgagttctt
    15661 gatcctctat tttcaagcaa tggtagaagg aatttctaag ccggagatat acgcaaaact
    15721 gcttccatat acagcagaat ggacagaagt cctgatcaag ggaatcgcac cgaagaagaa
    15781 ataaagcata aaaaagccga aactgacgat aaaatgtcag ttcggctttt ttggttcata
    15841 aatgacgtaa aatcaagcag attcgcgtgc ttctttgtca tagacatcat ggacattgat
    15901 gaatggatca agaataggat tttttccgtc taaatcagat tgaaaacgaa tgccatcgat
    15961 attgatccga gcatttcgat agaaatccgt acctaattgt ccccaagctg gtgtagaagc
    16021 atcgacatta cagaaaatat cgaaaccttg actcttcaag taagcaaatt tttgattttc
    16081 tgctgaataa ggctgccagt cgctgatgtc tgcaccaaat ggataaatca gaatatttgt
    16141 ttttcctagg ataggtgcaa cttcttgctg ccacaattca ttgtcttttt ttacatcttc
    16201 tagtgaagct tgagtcatat tcaagtgacc ccatgtatgg gaagcaaagc tccagccgtc
    16261 tttcttcaac tgttccgcca cttttttagc ttctttaatc tctttttttg ttttttcatt
    16321 gtctccatat tgagattttg atgttcgata acctaaaact ccgttgtaac ctgtcagggc
    16381 caaggtacct tttgcgcctc gataagaaaa atcaggatgt ttcttgacaa aggcatcgat
    16441 cagaggaacc atgtcaaagt caccgatttc ttctttatct ttctctttat agacattttt
    16501 gatttcattg ttatttgata cgaccaatcg gctaggaaaa ccggaattat ccatatattc
    16561 ataatagctg acatcatctt gtgaaagaac caatggtttt tttccttcag gtagcgcaag
    16621 cccgttaaag gaaagatttc cttgttcgtc tttttggatc aaatcattca aattaactaa
    16681 aacaaaatca ttttgataaa gttgttcgat cgaacggttg aattcttcaa cagtcaccat
    16741 ataatctttg tatccttgtg cctgttgaga gtggaaggct ttttgcgggt ccacgatcaa
    16801 gctgtggaaa aagacatgag agatctgagt aggatcagac cagtcgatca gttgttcttt
    16861 ctcctttttt agtgtttgaa gaagctgttg tgtttcagta gatccgttct tactatcttt
    16921 ttccaataac gcaatcgctt ggtcataatc gtagctttga gccagatttt ttgcttgagt
    16981 aactagttgt tgcttatcat ctgtcggttc ttttgctttt tctgttattt tttttcagga
    17041 atgattggtt ctttttttgt agatgatcgg atgacaaaaa acaaacttag aattaaaata
    17101 acgatagtta aagcagaagt ggctcctaaa ataatttttt gctttttttc catatacctc
    17161 gtcccttgct ttttcatttt tttacttgtc ccattcctta ttgtaacgaa gaaagaataa
    17221 gaggcaaagt aaagtgactt ctcgataaat tgcagaaaaa atacagaaaa agttatttct
    17281 tttaacaata attgaaaata aaatgttcgg aattagagcg gattcaaaaa agaaaaaatg
    17341 aggaatttaa aaaaaatatc ctttttctgt tgacaaagaa aaagttgcgc tctatgatag
    17401 acacatatta ataataacaa gacgaagatc gaaacgaagt agttgttaaa gccaatgttt
    17461 cagaaagctg gtggtcgctg caaaccagta catgctttaa agcgaattac atttcaggag
    17521 tctttggcag tgattgccac gcgacagcgt taaatgttcg agaggtatgt tggatatcta
    17581 gacatacaaa ttgggtggta ccgcgagatt tcgtcccttg gcttatatgc caagggactt
    17641 tttttatttc ctatcctatc ctgaagaaat aattaattac acatgctagc aggttcctgc
    17701 tttcataaaa aaactacacg gaggtcagta aaatgaatat tattgatgaa ttaacttggc
    17761 gcgacgccat taatcagcaa acaaacgaag aaagacttcg cgagctcgtt gaagaaaaga
    17821 gcatttcact ttattgcggc gttgatccaa ctggtgattc gatgcatatc ggacatttga
    17881 ttccatttat gatgatgaaa agattccaat tggcaggcca tcatccatat attctgattg
    17941 gcggcggaac aggaacgatc ggcgacccaa gcggccgcaa aacagaacgt gtgttgcaaa
    18001 cgatggaaca agtgcaacat aacgtgggcg cactttccaa tcaaatgaga aaattgtttg
    18061 gtaaagatgc aaatatcaca tttgtgaaca actacgactg gttgtcaaaa atctctttac
    18121 ttgaattttt gagagactac ggtaaaaact tcaacatcaa cacgatgtta gcaaaagata
    18181 tcgttgccag ccgtttggaa gtaggaatct catttacaga gtttacttac caaatcctgc
    18241 aatctatcga ctttttgcat ctgcataaaa catacgatgt gcaattgcaa atcggtggag
    18301 cagaccaatg gggaaatatc acagctggat tagacctaat ccgaaaatta gaaggaccag
    18361 aagcagaagc gttcggttta acgatcccat tgatgctgaa ggctgacgga acgaaattcg
    18421 gaaaaactgc aggcggtgcc gtttggcttg atccgaagaa aacttcacca tttgagttct
    18481 accaattctg gttgaatcaa gatgatcgcg atgtagtgaa atacttgaaa ttctttactt
    18541 tcttgtctca agaagaaatc gaagacttag cgaaaaaagt agagacagaa cctgagaaac
    18601 gtgaagcaca acgccgtttg gcagaagaag ttacaagatt tgtgcatagt gaagaagact
    18661 tgaaagaagc gcaaaaaata acgcaagcat tgttctctgg aaatattaaa gaactaaatg
    18721 cggaagaaat cgcgcaagga ttcggtaaaa tgccgaacgt tgaaatttca agcacacctg
    18781 aaaatatcgt ggaactgctt gtttccacaa aaattgaacc atctaaacgt caagcacgtg
    18841 aagatgtttc aaacggagct ataagtatta acggtgaccg cgttaccgat ttaaattttg
    18901 tcataaatcc atccgatgaa ttcgatggta agtttgtggt tattcgaaaa ggtaagaaaa
    18961 attacttttt ggccaaagta attgattaga atttagtaag tttttacctt gaaaaaggga
    19021 aatactatta caacattcca attttgaaac ccatcctaaa atagaacgct ggttgtcgtt
    19081 aatacaatct ttgcgaggga gtttacattt gtaaattccc tcgttttttt taattatttc
    19141 aaaagtgtga acaattcgga taattgatac atattaaaat atgattaaaa atcaggccaa
    19201 aacttagttt acagaataga agaacaggtg taatgtaggg ctcgacaaaa tttaaatatt
    19261 tttttaggag ggtttcacta tgagtgaatc attgtcgaaa gatctaaacc taaatgccct
    19321 attcattggg gacaaagctg agaacggcca aatctataaa gctttgttga atgaattggt
    19381 ggatgaacat ttaggctggc gtcaaaacta catgcctcaa gacatgccta ttattacgcc
    19441 agaagaaaaa agcagtgcta gttttgaaca cactgtgaat aaaacaaagg atgttctatc
    19501 agaaatttca gcgcggatgc gtacccattc cgttccttgg cataatgccg gacgttactg
    19561 gggtcatatg aactctgaaa cattgatgcc atctttacta gcgtacaatt ttgctatgct
    19621 atggaatggt aataacgttg cctatgaatc ttctcctgca acaagccaaa tggaagaaga
    19681 agtaggaatg gaatttgcaa aattaatgag ctataaagat ggctggggcc acatcgttgc
    19741 tgatggttct ttagcaaact tagaaggact ttggtatgcc cgcaacatca aatcattgcc
    19801 gcttgcaatg aaagaagtga cacctgaact agttgctgga aaaagcgatt gggaattaat
    19861 gaacttgtca acagaagaaa tcatgaatct gttagacagc gtcccagaaa aaatcgacga
    19921 aatcaaagct cactcagcac gtagcggaaa acatttagaa aaattaggaa aatggttggt
    19981 tccacaaact aaacactatt catggctaaa agctgccgac atcatcggta tcggtttaga
    20041 ccaagtgatt ccagtgccag ttgaccacaa ctatcgcatg gacatcaacg aacttgaaaa
    20101 aatcgttcgt ggtctagcag ctgaaaaaac accaatccta ggtgtagtcg gtgttgtagg
    20161 atcaacagaa gaaggagcaa tcgacggtat cgataaaatc gttgcattgc gtcgcgtatt
    20221 agaaaaagac ggtatttact tctacttaca cgtagatgct gcttatggcg gatacggccg
    20281 tgcgattttc ttagacgaag acaacaactt cattccattt gaagatttaa aagatgttca
    20341 ttacaaatac aatgtcttta cagaaaacaa agattatatc ttagaagaag tacacagtgc
    20401 atataaagca atcgaagaag cagaatctgt aacgattgac ccacataaaa tgggctatgt
    20461 tccatattct gccggtggta tcgtcatcaa agatatccgc atgagagacg ttatctctta
    20521 ctttgcgaca tatgtatttg aaaaaggcgc agatatccca gcattacttg gggcttatat
    20581 cttggaaggt tcaaaagcag gggcaacagc agccagcgtt tgggcagcac accatgtctt
    20641 acctttgaat gtcacaggat atggtaaatt aatgggtgca tcaatcgaag gtgcacatcg
    20701 tttctacaac ttcttgaatg acttgtcatt taaagtagga gacaaagaaa tcgaagttca
    20761 tccattaact tatccagatt tcaatatggt agactatgta ttcaaagaaa aaggcaacga
    20821 tgacttagtt gcaatgaata aattgaacca tgacgtatat gattattctt catacgtgaa
    20881 aggaagcatt tacggtaatg agttcttaac ttcacatact gactttgcta tcccagacta
    20941 tggcaacagc ccattacaat ttgtgaacca actaggattc tcagatgaag aatggaaccg
    21001 tgccggaaaa gtaactgtat tgcgtgccag cgtaatgaca ccatacatga acaaagaaga
    21061 acactttgaa gaatatgctg aaaaaatcaa agcagctctt caagaaaaat tggaaaaaat
    21121 ctacgcagac caattattgg caagcgaagc aaaataagca aggtaatcat gttagaaaac
    21181 agcttgaata ctaactgttt ctgacgcatg tttggatctg aagagcaagg ggagacctaa
    21241 gctcttcgat ccctttgttt tgaggttgtc tttgaagaaa cccttcaagc aataaactca
    21301 aagaatcccc tcaaatattc gctgaattgt ttcgtttatt gctgaaacta cttcaaagac
    21361 accgttattt tatccaaaaa atcaaggaga gtgtcaacgg tgagtaaatc tgaagtaagt
    21421 aaaatgggtc ttgggacctt tatcggtctg acaatggcac tgtgtgcaac ggttcggagt
    21481 attcctacgt tagccgcagt cggttggaca ttgatttttt attcaatttt tgcagttttg
    21541 ttttttgccg gtccgatatc aatggtttcc ggtgagcttt ccacgatgct tcctcaagaa
    21601 ggcggtcctc agttatgggt aaaaacagca ctgggcagca aatggggatt tgtcgttgca
    21661 tggctattat gggtgcaaat gttcccaggt atggtaatgg tcgcttctac tttaggacca
    21721 ttgttaggaa acacatttgg taatgtagaa cttggcaata atcactggtt cgttttaggt
    21781 tgtattttag ttatctactg gatcatcacg atcttgaact tgaaattcga tatggcaaaa
    21841 gtcggcggga acatcggtgt atggctaggg gtatatatcc cagtagtcat catgttcgtt
    21901 ttaggtgttt tggcagcctt caaagtcgga ctagtctcaa acggctatct aggtgacttt
    21961 tcatggtcaa aagctttccc tgacttggaa catattgatt cattgaaata cttagctggt
    22021 atcacattta tttttgttgg gatcgaaatg agttctgttt acatgcctcg tttgaaagac
    22081 gcaacaaaaa attatacaaa aggcgtattt atcgctttga tcggattagt tttattgaat
    22141 gtcatcaatg cgatgcttgt agcaaatgtc gtaccagatg gaaaaatgga attagcaaat
    22201 atcacgcagc ctatcttgat tgactgtcaa atcctaggct taccagaagt gatcggaaat
    22261 atcttcagct tcatggtatt tattggtgtg cttttacaat tatcagcttg ggttactgga
    22321 ccatcaaaaa caatcatcca agtagctcgt gaaggattcc ttccaccaaa attcggattc
    22381 cataaagaaa acaaatacgg cgtatctaga aatgtcgttc taacacaatc aatcgttatt
    22441 tcattatttg ctttgcttta tggtgtaatg gatgacgtaa gtgctgtatt cttgacactt
    22501 acaaatgcta caactgttat ttattgtatc gtgtacattt tgattgctgt ttcattgctt
    22561 aaaatgcgta agaaacatcc agaattcgaa cgtccttacc gaattggtaa aaacggaaac
    22621 ggattagctt gggtagtcag ctgcatgctg atcttctcta tcatcgttgt cgtatttgct
    22681 acattaggta cagcgacatt gagtgatgca ttattagtag cagctatcac agtggtcatg
    22741 ttcgttattc cattgatcat caatcacttc aagaaagatt cttgggggat tgaggtagaa
    22801 aaaagtttag aagaaaaata aaaaaataag tctttcagcc gacgttttgt cgctgattta
    22861 gtaggaggtc ctgccaagtg aatagttttt tcatggcagg gccttttcat ttgaaatagg
    22921 agatcgacga tgaataaaaa tatttcttta aaagaaagta tcgctgtatt gatcgtgttg
    22981 ttggcactcc taggagtact gatcatcgga ttccagctct cgccacatat cccgatcctt
    23041 gcagctttca tgtgtttgtt gttttatggc agatttaaaa agttcacatg ggatgagatc
    23101 catgacggga tcatcaaagg aatcactcca gggatcattc caatcctgat ttttcttttg
    23161 attggtgtct tagttgcttc atggatttta tctggaacca tccctacgat tatggtttat
    23221 ggatttaaac ttgtttctgt gcgtttcttt ttaccgacag catttcttgt ttgtgcaatt
    23281 gtcggagtga ctgtcggcag ttcatttaca acgatttcga cgattggaat tgcttttttg
    23341 gggatcggtc atttacttgg attcaatgat gcaatgacta caggcgcagt cgtttccggt
    23401 gcctttttag ggaataactg ctcgccttta tcagatacga ccaacttggc agcgggtatt
    23461 ggaggagtta atctattcga acatatcgct ggtatgcgtt atacggattt cccagcattc
    23521 gtcctttcgt tgatttttta tacgatatta ggacataaca gtcattcagc cgatctagct
    23581 agtatcaatg aaatgatcca aaacatgaaa gcgagctttt ggatctctcc ttggacattg
    23641 attccactag ttattttatt tgggggggca atcaaaaaaa ttccggctat ccctacatta
    23701 ctagcgggat cgacagctgc tctcgttctt tcttttatcc atgagaaaag tttgacggtc
    23761 ggaaaagcag cagacatctt gatgaatggt tatgtcgcaa atacacctga tccaaagcta
    23821 gatgaactgc tttctcgagg cgggatcatg agtatgttag gttctgctag tttgatttta
    23881 cttgcattgg cattaggtgg gttactgatc aaatatctga tcgttgatac catcgttcaa
    23941 gaattaaaag aaaagatgga ccgaccttct cgtttagtcg ggtttactgc cttgagctgt
    24001 atcggaatca atctgatcgt tggagaacag tatctgtcca tcattcttcc aggagaaaca
    24061 ttcaagcgtt cttttgatga cgttggctta tcaaaaacct atttgacacg aacactagcg
    24121 gattcaggtg cgaccgtcaa ttctctcgta ccgtggggag tcagcggcac attcattatg
    24181 ggaacgatga aagttagcgc gttgcattac ttgccattcg cctttttctc gattcttgca
    24241 cctgtcttta caatattagg cggtttttta ttgaatcgtc gacgtgtaaa aaattgaagg
    24301 aaggaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag aaactcaaga
    24361 aaattgcttt t
//
LOCUS       DACECR010000040        23128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.040, whole genome shotgun sequence.
ACCESSION   DACECR010000040 DACECR010000000
VERSION     DACECR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.040"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(174..1589)
                     /locus_tag="IWO17_001437"
     CDS             complement(174..1589)
                     /locus_tag="IWO17_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0290320.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(1606..2922)
                     /locus_tag="IWO17_001438"
     CDS             complement(1606..2922)
                     /locus_tag="IWO17_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0290321.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(3072..3770)
                     /locus_tag="IWO17_001439"
     CDS             complement(3072..3770)
                     /locus_tag="IWO17_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ0290322.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(4059..5048)
                     /locus_tag="IWO17_001440"
     CDS             complement(4059..5048)
                     /locus_tag="IWO17_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ0290323.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(5128..5961)
                     /gene="msrA"
                     /locus_tag="IWO17_001441"
     CDS             complement(5128..5961)
                     /gene="msrA"
                     /locus_tag="IWO17_001441"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0290324.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(6136..7770)
                     /locus_tag="IWO17_001442"
     CDS             complement(6136..7770)
                     /locus_tag="IWO17_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290325.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(7757..9604)
                     /locus_tag="IWO17_001443"
     CDS             complement(7757..9604)
                     /locus_tag="IWO17_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290326.1"
                     /translation="MFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKDNSSILKI
                     ELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSS
                     IFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNICTILI
                     SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLI
                     FNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLV
                     KAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTM
                     GLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWR
                     ILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHG
                     HIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNI
                     TYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKY
                     TPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            9591..11228
                     /locus_tag="IWO17_001444"
     CDS             9591..11228
                     /locus_tag="IWO17_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0290327.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(11373..12113)
                     /gene="truA"
                     /locus_tag="IWO17_001445"
     CDS             complement(11373..12113)
                     /gene="truA"
                     /locus_tag="IWO17_001445"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0290328.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(12304..12939)
                     /locus_tag="IWO17_001446"
     CDS             complement(12304..12939)
                     /locus_tag="IWO17_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0290329.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(13086..14327)
                     /gene="sstT"
                     /locus_tag="IWO17_001447"
     CDS             complement(13086..14327)
                     /gene="sstT"
                     /locus_tag="IWO17_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ0290330.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            14525..15523
                     /locus_tag="IWO17_001448"
     CDS             14525..15523
                     /locus_tag="IWO17_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290331.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            15536..17554
                     /locus_tag="IWO17_001449"
     CDS             15536..17554
                     /locus_tag="IWO17_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAQ0290332.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(17614..18330)
                     /locus_tag="IWO17_001450"
     CDS             complement(17614..18330)
                     /locus_tag="IWO17_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0290333.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(18557..19099)
                     /locus_tag="IWO17_001451"
     CDS             complement(18557..19099)
                     /locus_tag="IWO17_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0290334.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(19104..19331)
                     /locus_tag="IWO17_001452"
     CDS             complement(19104..19331)
                     /locus_tag="IWO17_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAQ0290335.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(19410..21290)
                     /locus_tag="IWO17_001453"
     CDS             complement(19410..21290)
                     /locus_tag="IWO17_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0290336.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(21479..22093)
                     /locus_tag="IWO17_001454"
     CDS             complement(21479..22093)
                     /locus_tag="IWO17_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0290337.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            22235..22795
                     /locus_tag="IWO17_001455"
     CDS             22235..22795
                     /locus_tag="IWO17_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0290338.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         7225 a         4501 c         3614 g         7788 t
ORIGIN      
        1 gttgttttta ctttgctatt cttattactt gatgcaggac agctttttca caacctctca
       61 ataaacggtg ttcaatcagc tgaccctggg tattagctca aaacgctgga ttcacaacct
      121 cttttatttc aaataaccag taaatacaat gaacatctac aaaaaacttc tatttattta
      181 tagatttcgt caggcaaatc caattctctc ttatcaatca aagtctgata ccacgaagca
      241 gattttttca atgttctgct tcgttcatcg ttcaattcga ttcgaactaa tccatatcga
      301 ttcttaaatg cattcattgg tgacacacag tcagtgaatg cccataacat atagccttcg
      361 caatttgcgc cttcttctgt tgctttgatc aagtaatgca aatgctgtcc gataaattcg
      421 atacggtaat catcttgcac gattcctttc tcatccatat actgttcttc atattctctt
      481 cccataccgt tttctgttat cagccaaggg atattatgat aattttcttt tagatacatg
      541 gctatatcat acatgatttt cggatagatt tcccatcccc tggaattatt cattttcttc
      601 ccaggtaaat caaagacttc ataatatcgt tcaggatgga aaggtgtctc ttcattccat
      661 tgataacgag gggcgcgaac gcgtttagga taatattgat tgattccaag gaaatctact
      721 gtatgatgct gaatcgtttc taaatcctct gttgcaggat cgaaataaat cgattgtttt
      781 tgacacaact ccaggaactc ttctgtgtag tatcctttta ccatcggatc aaaaaatact
      841 cgattataaa acaaatcata catataagca gctttttggt cttctggaga attggatcga
      901 gcatagacca tttcaggatt taaaatacag ccaatccttc caccataatt tccgctgcgg
      961 taaatctcta ctgctttagc cgttgctaaa actttatgat aattccacat catccacttt
     1021 tttgtgtttt gttcatgcgg ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg
     1081 attggttcgt tgaatgtaaa ccagtttttt accctatcac cgtaacgatc aaaggcaatt
     1141 tgagcatact tagcataaaa atcaactact tttcttgaag accagccatc atatttttcc
     1201 aaaagaaaag ctgggacttc ataatgttcc aaacaaatca tcggttctac gtctgcttcg
     1261 atcaaagagt cgatcatttc acttatatgc tgagcatatt cttcatctac tatacagttc
     1321 tcataatcag taaaaaaacg agcccaattg atagaagtcc gataatgtgt cagatggatg
     1381 gatttcatca gatccacatc ctgctggtat tgttccataa aattagttgc tacggctggg
     1441 ccatacccat tatgccaaac aaaatgttca tttttatacc agctatctat aaacgaatcc
     1501 tgtccttctt tttttccttt ccaaccttcc gtttgccaag cagaagatgc agcaccaaga
     1561 ataaaatttt ttggaatagt aattttcatg atttcctcct gttttttaga tcccatcatt
     1621 ttccacacga tttgctactt ttacaaatgg cagatagatc aaaatagaaa caataataca
     1681 aacaagttgc gtaataactg caccgatatt tcctgctgtt gccagataag cattgacgat
     1741 cggcggcatt ggccaaggga ccattacaac tgctttcgcc gcaaatccta aagcggttgc
     1801 aaaataacca atcgttccag tgataagagg cgttaagata aacggaatag ctaaaattgg
     1861 attcagcatg atcggcatac caaaaataac tggttcattg atgttgaaga tcccaggagc
     1921 aagggataac ttggtgattt gtttcatatc ttcccgtttt ccaactaaca atactgcaat
     1981 cagtaaaccg atcgtgactc cagacccgcc catactcata tacatatccc aaaatggcat
     2041 agtaataatg ttcggtactt cttttccagc ttcaaaagct tccgtattta cagcaattgc
     2101 cgctaaaagc aacggttccc gtatcggctt caccatttga tttccatgga taccaatcac
     2161 ccaaaagact tgcgcaataa acatcaatag caaaataccc ggtaaccctt gtacgacacc
     2221 ttctaatggg cgttgtacga cattgtaaat gatatcataa aggtacattc ctgtaagttc
     2281 tttgatgata aatcccgcag ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc
     2341 agaaaaacta gcagaaacat tcgttggcac ggtatcaggc attctcagct gtagtttctt
     2401 ctgtttcctc aaccagcaat aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa
     2461 taaccctttc gtatctgtat attgtttagc caatacgttt tctactataa cagtctgatt
     2521 ttcattcact aattcataaa cagttggatt aacagataaa taagacatca ctgcgatgat
     2581 accagggaat acaccatcca ctttattcag cttggctaat tcgatcccta tcagaaaaac
     2641 agcatagatg gtcaagaaac ttagtgtgac atagctgatg gcagatgcga taggctttaa
     2701 ttcttctaaa aaacgcaaga aacttatctg tgctaagcca ttttccgagt caaagaccat
     2761 tgctgaaaac aatgtgccaa aagcacccgt aataattacc ggaatcaatg cagcaaatgc
     2821 gttttttatc accatgatat accttaaatt attgattctc gttgccacgt tccctaaaac
     2881 attggctaat ttatccataa aactcattcc agatcccctc attcctgctt cttctccttg
     2941 acgtcatctc tttttgtcta ttagtcagcg cttacatttc gatgaacaga aactatttac
     3001 tcctcttttt taccaaatat aaaacaaaaa ttcatctgat gtatttatca taaaatacat
     3061 cagatgaact gtcaattagt ttctttttct cctattattt tctgtaattc tctccgattt
     3121 attgccatat ggacagtcat tgcatcaaac gcagcaagtg cattccgcct ttcgatggcc
     3181 tgaacgatct ctgtatgaat atcgatcgtc tcgattttca gatgctggat atcataattt
     3241 ttattaaaca aagcaattga ttcattaatg attgggataa tatgcagcaa ggctacgttt
     3301 ccgctagctt ctgcaatcat cgtatgaaaa tcgatgtcta attgaacatg ttcttgtgtg
     3361 ccttctcgaa aagaagtttc aatcttttgc gttaattttt tcatttggac gatttgattc
     3421 ttcgttgcat attccgctgc caaacctgcc atcttaggct ccaataaata tctcagttca
     3481 aataagtctc gtatcatccg atcctgatct tcaatcaaag aaaaaccgag tgggtcattg
     3541 gcaatccctc gcttatcact gacaaatgtc cccgctcctt gccgaacttc aaggatattc
     3601 ctggaagcta gagcccgcac agcttctcgc accgtgcttc gccccacctc taaataatcg
     3661 gacaattcat attcattcgg cagtttatct cctacttgca actgcatttc ctttaaatat
     3721 ctaaggatct tgtcagtagt tacttctact agggttttat tttcttccaa accacatacc
     3781 tccttttcat ctgatgtatt ttttcgtaat tattataaca cgtttgaatc atctgatgat
     3841 tttttgtata caaaattgat taatccgttc atagacatac aaacgaagaa gatatttacc
     3901 ataaaaaaga ctaggataaa attcactcct agccttcgat catttttatt ttgaacgaca
     3961 aaaaatatga tgttcaataa cagacgatct tcattcgatt cttgatgtca tcttttgttt
     4021 tttttaatat atggataaaa agtttgatta catcgtttct attctagaat ttggtcgatt
     4081 ctcaattctt cttctggtga gaaatctaaa ttatccatca ttcgaacact ttcttgtaac
     4141 tgacttaacc gacttgctcc gaccagtaca cttgtaacag atttttgtcg caaattccat
     4201 gcaagggcca tttgcgctaa tgattgtcct cgtgatacgg cgattgtttg cagtgctttg
     4261 attttttcca gagttgatcc aacttgttct tctgaaagga atggtatttc cttacgatgt
     4321 gctctagaat cttcaggaat accatgtaga taacgatttg tcagcaaccc ttgagcaagc
     4381 ggactgaacg tgattgcccc tagcccttct tcttctaata ctggagatag atcgtcttct
     4441 agtagtgcac gagaaaacat attgtagcgc atctgatgaa taatgaacgg tgcttccttt
     4501 ctttttaaaa tttcagtcat tttttttgta tcttctccat tatagttgga tataccaata
     4561 tataatgctt ttccttgtct tacaagttgc atcaaagctt ctgctgtttc ttccaaaggt
     4621 gtatcagggt ctgggcgatg atgatagaag atgtcaacat aatctagccc cattctttgg
     4681 agactttggt cacaacttga aatgatggat ttacgagagc cccattcccc atatggccct
     4741 tcccacatat gataacctgc tttgctagaa ataatcagtt catcacggta ggcttgaaaa
     4801 tcttctctaa aaatacgccc aaagtttttc tctgcagctc cagcagggcc tccataatta
     4861 ttcgcaaggt cgaaatgggt aattcccatg tcgaatgcgc ctcttaaaat ctctctttga
     4921 ttatggatag gatcataatc accaaaattt tgccataatc cgagagataa aatcggtaat
     4981 ttcaaacctg aatgaccgga tttacgatat tccatttttt cataacgtgc actatctgca
     5041 atatacaatt tttctcctcc tcatcaaatc tttttaaaac gctttctttt ttctattttt
     5101 agtttactac attttaaaga aaaggagtta atcaaaatga ttttttcgta tatttttttg
     5161 ccatttgttt tttactcgtt gatagatcag aaattgatta cgtgcttgct ggattttttt
     5221 ataacgtttc ggttgttttt tgtaaaattg ctgatgatag ttttctgctg gccaaaacgt
     5281 accggctggt tcgatttttg tgacgattgg ttggtggaaa gttcctgaac tgtctagttt
     5341 ctgttttgat tgttccgcta attctttttg tcgttcgact tgataaaaaa tcactggacg
     5401 atattgcttc cctctatctt gaaattgtcc aaatgtatct gtgggatcag taatttgcca
     5461 atagattgtc agcaactcgc tataacttat ttctcttgtg tcataaatga tctccacagc
     5521 ttccacatga cctgtgtaac caccacttac ttgatcataa tttggttttt caacatgtcc
     5581 gcccgtgtat cctgacaaaa cagataggat cccttttttc gattcaaacg gctcgaccat
     5641 acaccagaaa catccccctg caaatactgc acgttcttga tagtcagcat cttgcatggc
     5701 atgcttggaa aagtcatatt tttgctcctt atcaaatcct tccgtaatca ataaataaaa
     5761 gtccattaca tcaggcgtca gattcattcg gatagctaat ggacgtagtg ttgcttctag
     5821 ttttgataac tgttctttaa cggatatatt ttctcttatg tcattctttg tctgaatcaa
     5881 tacttttcgt tcccaatctc gtgtattcgg attcaaaatc agattataaa gtgtttgaat
     5941 gaatatttgt tcctcatcca tagattctcc tcctttccga agaagacaag ctagtgactt
     6001 tagctgtaat ttatcataaa gtgaggaaat aagcgattac ttcatccccc tacagatttt
     6061 tcgtataaaa aagagagaat ccttatcgac agattctcct ttttaatcaa tttattttat
     6121 aaatgaaaag ttaatttacc ttttatggaa aactatttta aaaaataaac ataaatatgc
     6181 agttaatgta acaagataag aaataatagt cgttactgta taacttgtgc ttactttaat
     6241 atcagcaact cctttgactc cgcttatttg taccaatcca ttagcattca cctgctcctt
     6301 tacaggttga tgatttattt ctacattcgt tcctaaataa gcatatacag gaagtgtgat
     6361 tacttggctc ttctcattag ttactctata tatgatagaa ctaccagttg ttttataagg
     6421 aaggtttacc cattgactgc caatctttgc ttcgtgtttt attatatgct ccttattctc
     6481 caatgaagcg ataggagaat aatcatataa aaaataatcc attcctttag tggtatacgc
     6541 ctctactgtt tctttgttta caacaatatg tcccggctga gaaattattt tttgattgaa
     6601 atttttttta ctagaaacag ttaaagtgat taataacaaa acagtcataa ataaataaaa
     6661 tagttctctt gtcttattgt atcttctatt tttgaaaaat atcatcgtta atatcagaga
     6721 agagaatatt atttgcaagc ttaagatccg ccaaggaaat tgtaacaatt tggctggcgt
     6781 attttgcaga agataccatg gcaccaaact agttgtagct agccaagtca aacacgtacc
     6841 ataataaatc tttttttcta tgtttgttaa ttttttaaat aaaatcaggg gaatacataa
     6901 agataacaga ccaactagtc ccattgagta agtagttaag tcgttgttca tagcttcagt
     6961 cagactatcg aacacattag atgcatgagc atataaattt gtcacgctag gtttgttgat
     7021 tttttggtag agcatttgtt caaacatcgg ataccaaaaa gcactcgtca gaaccaatgt
     7081 cattcctgcc gcactaaata attttttaaa tatagcaata aaatctgcat tcttgaaacc
     7141 tttagaaacc aaacgaataa aaataaaaat gataattaat gctgtactca ttaatgttga
     7201 tagtacatgg ctgtacacta gcaaactcat actcaatgct agctttacag acggatactt
     7261 ttcttctttt ccagagatga tttgataagc ataaaataat acagggataa caagtgttat
     7321 tgcgatagct tcggcaattg cggaacgata gtagatgtct actgtccgat aagcagagaa
     7381 aatatagaga caactaaata aaaatgccgc ttttttttgt tttaaaaacc gctcgccata
     7441 atgataagct acgagtgttg tacacaagtt caataaccaa acgtaaataa tatagcttac
     7501 aatcaaattt tttgtcatcc aatataacat tacagctgga aaaaaagtca gaaagggata
     7561 aaaataattt acaccatatc cagtataatt gaatgtctca taattaatcg gactggtaaa
     7621 aatagtcgat aaccccttga ttcgagataa gtgaaaagaa agatctccag tggtataaat
     7681 actctgtgta tttttgaaaa agatgaaaga atatatacta ctaactataa ttaatccact
     7741 caatattact acttttttat ttattttcat tcttcttgtt tatctccttt ttagtcacat
     7801 atcgagaatc tttcaactta ttaggtgcaa atcgtttaaa acttgaatac accactaaac
     7861 aataagtcag aatactgatc cataaagcca tgagagcagt tggagtatat tttgttgtaa
     7921 tttctatctt attttctcca gcatttacat ccacattcac taagccatgt tttttataag
     7981 agtcatttac gactttaccg tttactttta cctgtgtacc taaataagca tatactggta
     8041 ctgtcacttt ttgctttttg ttgcttgcca ctgtgtaagt aatgttatta tctgcagctt
     8101 tatacttccc tttttcccac gattctccta cttttacttc atgtttttct aagtgaggcc
     8161 tttcttttat cgcttctgtt ggggcgtaat cgtataatcc tcccatatct gatgttgtat
     8221 aacgtgggag tgtttcttca ttgataacaa tatgaccgtg ttttgattgg ataacttttg
     8281 cgtaattttc ttttgctgct atagacgtga cgagtagaaa caccacagta gcgccaatga
     8341 ttaaccatga atatttttta ttcatagtct ttttcataaa aacaagcgtt atgatcattg
     8401 aagaaaaaaa gatttggata cctaagatac gccaaggaaa ctggagtaag cttatcggtg
     8461 ttttttgaaa caaaaaccat ggaaataaat tagttacgca tacccatgta gaaactacgc
     8521 caacatagat gattttttct tttttctcca tttctttcag tatgaataaa ggaaaaatca
     8581 acgcaaccaa tcctaataac cccatagtgt atgttgtaat atcgttattt aacgcttgaa
     8641 tgatactatc tgaaatagaa agtgcttcta gctgcaaatc tgttttgaag ggactgtgta
     8701 tttcttgata attcatctgc tgcaacatag gataccaaaa atagctagtt aatgcacaag
     8761 tcaaaaaaac agcctttact aaattcacga ccacaaattt tatatcgttt tttatttgct
     8821 tgcttaaaaa caaacgaact ataatcatac aagcaatcaa tgcactacac aatagtgtag
     8881 ttaagacatg agtatacaca agtagactta tactgacaga taaagcgatg actttttttt
     8941 ctctattgaa tatcaatcca taagcataat ataaaacagc tgggataatc gtaagagcga
     9001 ttgcttctgc aattgcggat cgataataga tatcaactgt tcgataagtt gcaaaagtat
     9061 ataaacaact aaataaaaaa gcagcatcta tctttttaaa aaacttttct ccataataaa
     9121 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
     9181 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
     9241 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
     9301 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
     9361 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
     9421 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
     9481 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
     9541 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
     9601 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
     9661 ctctattcat gtctaaggga atctatcata gtgtcaatca ggatacttac tttcatttat
     9721 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
     9781 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
     9841 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
     9901 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
     9961 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
    10021 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
    10081 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
    10141 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
    10201 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
    10261 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
    10321 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
    10381 tgacacatat tttaaataat aactataaca actatactat tggattattc ttatttctcg
    10441 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
    10501 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
    10561 atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct tctttattta
    10621 tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat aattcatgga
    10681 aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta agcaattcta
    10741 tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt gctatagctg
    10801 aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct gatatgatta
    10861 ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat tttatgagta
    10921 acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg acgacaccta
    10981 tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa acaaaattat
    11041 ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc gttattactt
    11101 atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact ttgattttat
    11161 ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa gtacaacatt
    11221 ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag gctaaaaagt
    11281 ctttttttat atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg
    11341 aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac
    11401 agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca
    11461 tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc
    11521 cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt
    11581 cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca
    11641 gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc
    11701 atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg
    11761 ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc
    11821 tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag
    11881 tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc
    11941 gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc
    12001 tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc
    12061 catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag
    12121 tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata
    12181 tagctccttg cttctctaag acagaagcta tacaagaaaa ctgtggaaat atatatttca
    12241 gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa
    12301 gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa
    12361 actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat
    12421 tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa
    12481 gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa
    12541 taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca
    12601 cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata
    12661 gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactaa caaatgcagc
    12721 atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa
    12781 cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc
    12841 taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa
    12901 taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt
    12961 acctatgctt ttaattgtta ttataaacga ctttatcttg atttaacaga aacctctatt
    13021 tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct
    13081 aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt
    13141 cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag
    13201 gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc
    13261 gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg
    13321 cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc
    13381 aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg
    13441 gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac
    13501 tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa
    13561 cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag
    13621 ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc
    13681 atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc gattccagtc
    13741 gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact
    13801 tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa
    13861 cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca
    13921 tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt
    13981 tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa
    14041 attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc
    14101 aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc
    14161 aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat
    14221 attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca
    14281 acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt
    14341 cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa
    14401 aaattctaac aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt
    14461 tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt
    14521 tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga
    14581 gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt
    14641 gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac
    14701 atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca
    14761 atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt
    14821 cctaatagcc acactagttt gcaatatctt tttttacgta caagctggca gcttgaatat
    14881 tcgtgattat tggcttatcc taacacctat cagtcagaat tactttactt atgctgtttc
    14941 ttgtactttg cttttttgta gtattcctta tacagttgga tttttaaata aattatcgaa
    15001 aacaacaatt aaatatctac tattattttt aacaattgga ttagttggct gctctactct
    15061 atttaataaa gacccgtttg cattacagaa tggacagagt attttatgga ttttttattt
    15121 atttttaatg ggttatggtt tgaaagaatg gaactggaaa gaaaaggtcc gctataagct
    15181 tcctcaagta tttatcagct tgattgtttt attcggtttg attattttga tgacacaaat
    15241 ttcactcatc atcagaggaa atacagatac agcgctgagg ttttctaaac catattctct
    15301 cttcgcaatg tattatactt tctcttcgtt tttattttta gaaatgctat ctgataaatt
    15361 aggtataaaa ggaagaggaa attttcttgc tgtatttttg attgtcactc aagttatatg
    15421 taatttccct ttagtttctt atcgcgtatc tacatttatg aaacgtgaat ttccaaactc
    15481 agggaaggca tggctgataa atatcgggca gttttttatt tgatatttat tagcagtgtc
    15541 cctcctagtt atctttttat tttatataca aaaaatccgt ttcattcatc gtttcatcga
    15601 atacttcgct tttgattcga ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg
    15661 gctttttgtt cataaaagtt attttttagt tgcagcattt ttttactgct ttaccttcgt
    15721 gcagattttc gttttagaac caaaagaagg ttggaaagaa actatccagg ttgccttaaa
    15781 aatatttact cagcgacaag cacccatgat cctgacaatc atcattatta tgggattttt
    15841 cttcttacta ttgttgattt ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat
    15901 taacttactg ctcactattt cgacagtaat caaaatggaa atgcgggaag agcctgtctt
    15961 tccttcagat ttaaaaatgc tgaccggatt gtcagaacta ttatcgatgg ttagcccagt
    16021 cttattaatc gtgggcggat tgatccttct cctattattg attactagta ttattgtaca
    16081 acgtagacta caaaaacagt attccttgaa gattcattgg aaacgtcgat ttatcagtat
    16141 cgctgtatta ttaggatgtt tttcgggtgt ctttttcatt aatcacaaaa attcgccttc
    16201 tttcttgctg tttaatttat ttaaagtaaa caagactttc tttaaccagc aaggcgccgt
    16261 aaaagataac ggaccagtca ttcaattcct aaacaatatc gacattaaaa tcatggaaga
    16321 acctgctggc tattcgaaag aaaaaattaa tggaataatg aaaaagtaca acacaaaggc
    16381 agatgaaatc aaccaaacaa gaagtgactg gttaaacaat cagacgatcg ttctcaattt
    16441 aagtgaaagc ttcagtgatc ctgcacgtgt ccctaatctg acagtaccta cagaccctat
    16501 tccttacatc aaaagcatta taacagaagg aacaggagga ttgatgttat ccgtcggtta
    16561 tggtggcgga acagctaaca tggaatggga aggactgaca ggcttgagta tcagtaacct
    16621 gtctgcttcg ttagttacac cttataccca gctagtggag cgtcagaaaa taagtccaaa
    16681 tattaccgat ctattcgatg aaaaaatagc catccatcct tttacagctg ctctttatcg
    16741 cagaaaacaa gtgtttgata aatttggttt tgacaggttc tattatttag acagtccgga
    16801 taaattaact tatacggata aaatagaaca aagcccacgc atatcggatg attcagcata
    16861 caaagaaacc ttgaaagctt tacaaagcaa tacggaaacg tcacaattca tccaattatc
    16921 cactatgcaa aaccatatgc cttatgatga ttactatagt gaattgaact acactgctga
    16981 aggaactgct gtcattccta atagaagaaa agaattacaa acttatatgc aaggccttca
    17041 ctataccgat acagcagtaa aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat
    17101 tacctttgtt ttttatggtg accatttacc cgcaatctat agtgggaatg attcgaaaaa
    17161 atatggatta gaacagcacc agacagatta tttcatttat aacaacaagt atagtcgtga
    17221 acaagcagct acgttaaata aagaagttgt tgctcctaat aactttggtg ctatggctct
    17281 tgagcatgcg aatatcaaag taactcctta ctatgcgttg ttgacagaag tatctaatca
    17341 tctacctgca ttaacaattg atcctacaga aagcttgtct aatcgtttta atggaaaaca
    17401 ggtctttgtt acagaggata gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa
    17461 agaaattcta gaagattatc gtctgataca atatgatctt gttgcaggta accagtacag
    17521 tgcaacatgg gcgacacaaa aaataaaaga ctaacaaata aactgagcaa ggggttgtga
    17581 taaaagacat gtcacaaccc cttgtttatc tcattaattt gcttgtccat aaacgatttg
    17641 attaaagcca tttttcaatt cattattcgc tgtctcgttg aagtaaagat aattatcggg
    17701 attcccctga ttttgttgtg tcaccaatac ttttggctga ttttgctgaa acccgaatag
    17761 atagacgtgc tttttcagat atggttctgt ctctgcatcg gaatagacgg ctgtgatttg
    17821 atagtctgct tgacctgtcc cactttctga ccattctgca gaaacaggca ctccaccaat
    17881 agccatcttc cactccccgt tcaaaatagc ttgagggact ttaagaccat ataagtccac
    17941 actcatttga ttcgtataac ttttgtattg ttgtcccatt gtttggcccc atttggtcat
    18001 aaatgcctct aattgtgcgg atttgtctgc attccacagt gtttgttcaa cttgttcgct
    18061 gctttctgaa gaggaagatg aagcctctac agaagaactt gaagtagatg tttgagttgt
    18121 ttcagtcgtt ttggaagact ctgacgaagt agaggtatct gctgtttctg tacttgctgc
    18181 agttgacttg ctctctgtgc tagttgttgt agtagttgca actgttgctt tcgctgtttc
    18241 ttttgttgat tcttttttcg attcattccc gttggtacaa ccagctaaca aaaaagctag
    18301 accaattgtt actaaaatcc tttttttcaa ttaaaaaact cctttcgatt actcccactt
    18361 ctttattcta tcaaataagc aatgtgattt tattgatttt tcgtattttc tcatcaatct
    18421 ttcaaatctt ctaaaaactg agaagcaccc gctgatgttg tctgtaatac atcagcgggt
    18481 gcttctttta ttttaatctt cgtgattttc caatttacct cacgctttat atttgtattt
    18541 gaagaaacat agacttttag tcatcatcgt cttcctcatc caaatcttgt cggacagttt
    18601 ttccttggag tctttgtact tggtagatga tttctttatt gaaacttaaa agttcaacaa
    18661 gcaattgttg caagccatat ttttcttctt tttcagctaa tttgattgcc cgtgtgataa
    18721 agacattttg gaaatcaaaa tcttttgcaa agttttccaa cattgttgag gctggtaaat
    18781 atttgttttg accatgttcg taaatttttc cataacgcat gacttcttct aaagtagacg
    18841 gaacgatttc attttcaatc actaactcat gagagatttt attgaaccat ctcgcttctt
    18901 gatcagcata ttctgaaaaa agggtactta attcttgcga agatgtaccc aaaacataca
    18961 attgtgcttg gatcaatttc atttcttcta atttaaaatt tgctaataca tgccccacca
    19021 tggcgccagc agttggttta tgatggtcga tatctgcttg ttttgtttct tgaacaaatt
    19081 tttcttccac tatattcatt gatttacgcc tcttttactt ttgctgattg gacttcataa
    19141 cctaattttt ccacaactgc agctaattgt tcagggtgga cttcatctgg atcaaagttt
    19201 gctttgattt tgctcgcatt aaacaaaact ttcgtattgc ttaccccagg ctgttgagaa
    19261 accgcatgtt cgattttttg catacagctt ggacaagcta gtgtatctaa ttgcataata
    19321 atttttttca tcgtagttct tccttccttt ttcttgatag tcttattcta gattacagct
    19381 tccataaaaa aattgattct tatcaagttt tacgattttt cttctttgct ttcgtgtcca
    19441 aaatacaata aacgcatcgc gttaaagata acgatcaaga tactagcttc gtgaacgaac
    19501 atcccactag ccatgtaaat aaacccggca aataagccgt tgagtaaaaa taagacgaca
    19561 ccaatggcaa tcactacatt ttcacgggta tttaaaacag ttttcttagc caagcggtag
    19621 gcatgaacca acgattcaaa attagattgc atcaagacaa cgtcactcgt ttcgatagca
    19681 acatccgttc ctgatcccat agcaatcccg atatcagccg cagccaaaga gggagaatca
    19741 ttgattccat ctccaataaa agcaacgcgc aatccttgtt cttggtattt tttcacaaat
    19801 tgaacctttt cgtctggtaa taattcagca tggacttcat caatgcctaa catatcagct
    19861 acataatcag cagtcatctg attatcacct gtcagcatca ctaactgttt tgctcctgct
    19921 tgtttcaact tagctaattg ttcagcgact tctggtcgaa tcacatctga cacacctatg
    19981 atttgagtga tttttttgtc gattgctaca ataatgaccg agctgcctgt ttgttgtaat
    20041 tgatgtaaat cttgtacttg ctttggtgtg agagaaagtt tgtgagcggc aatcaatttg
    20101 gtgttgcccg cacaaactgt atgaccatca atctcagctg tgattccttg tcccttgatt
    20161 gtctgattat ctttgactgc taattgttga aaatcaatgt ctttctcctg agcataatgg
    20221 atcacggcgc gccccagagg atgatcggat aacgtttcta atcgagccac taaagctaag
    20281 ctgtcccctt catcctctgt atagttcttg atagtggata cagcagtagt tcctttggtc
    20341 aatgtaccag ttttatcaaa gacaaaagta tcgattttgg aaaaagtatc cattgcttca
    20401 ccgcctttga ctaagatgcc ccgtttcgct ccattaccga ttcctgctac attcgaaaca
    20461 ggcgcaccaa tgactaaagc gccaggacaa ccaagaacca aaacagtgat tgctaaacga
    20521 aaatcacgac taatcagacc aacaaccaac gcaataagta aaacggttgg agtataatac
    20581 tgagaaaatc gatcaatgaa tttttccgca tgggatttgg tatcttgtgc ttcttctact
    20641 aattcgataa ttttggcaaa ggtcgtatca tctccgacct tagttgcttc aatcgtcacg
    20701 tagccatctg ctaaaatcgt tccagagaag acttcatctc cactgatttt gacgacttgt
    20761 cgtgattcac cagtcactgc ggcttcatcc gcataccctt tcccgtcaat gactcgtccg
    20821 tccacaggaa tagctccacc agtttttact aagacgatat cgccttcttc cacgtcatcg
    20881 atttccactt cttcagtcgt tccatcttct tggacaacac tagcagtaga tggggccatc
    20941 tcggtcaaag ccttgattga ctcccgcgtt ttggtcaatg ttttttgttc cagatagcta
    21001 ccaaacaaaa acaagaatgt aacgatagct gactccttgt attctccgat cataaaggca
    21061 ccaagcacag caatagtaac gagtaaatca attgagataa ctttgacttt cactgcctga
    21121 tacgcatgga tcataatagg tatgaccgca ataatagaag ctatggctag agcaatgtta
    21181 tagaacaaga caaaaccagt aaattttccg ataaaaccaa aaataatcaa cacagctgat
    21241 aaggcggtga tatgatttcg atgctcttgg atccactttt gtaatttcat ctttctccct
    21301 cttttccttt cattcttacg agactaagta tagatactta tccatcataa aaaattgacc
    21361 actatcaagt ttagtcaaaa aggcggtttt cttttggtag aatacactac aaaaaatagt
    21421 tagcaagaag actcactaaa agtgatcttg ccaactattg tcgtttctat cgaaattttc
    21481 attgaatgat tttcctgtac agcatacatc ggagatctat ttctttatct aagattttta
    21541 tcttgctaat tttttccttt agtctttcaa atccacgtcg ttcatagaat tgatacgtac
    21601 agccactatc tgtaaaaagg aatatttctt ttcctttctc tcttttttca aattctgtta
    21661 gcaacttggt accgatgcct tttgttttca aatcaggatt tgcagctaaa aaaatgatct
    21721 ctccatctgg cgaatgcttc tttaaatatg ctgcaagcat ttgttcattt gctgtctcgt
    21781 aagctccttt actttcttta gcaaatatat tttgtaaaaa atcgaatatc ttaacatata
    21841 tttgctgtcc aaaagaacga tgtttctttt tttctccttt tatctccgcc agcaaaacac
    21901 ctgccaactc gtcctctgca tacaaagcaa gaatttgagt tgctcgattc aactccatat
    21961 acaaaaaata tcttccgtaa agattcagca gaagttcatt ttccatatat ctctcaaagt
    22021 tcattccttc aacagcaaaa tagatagctt ttttgtaatc tttcttttct aagggtttaa
    22081 aagttagttt catcatttcg cctcctcaac ttcaatgatt gtgttatact tacattataa
    22141 tagacacagt gtctactatc tataatcaat catctataag tttgtcgatt aagcgacagc
    22201 tttgctttga tatgtcggat aaggagatag gtaaatgaaa aagacagata gacgcgtcaa
    22261 aaaaacagaa aaagctttgg ctgaaacact atccacatta ttagtaaata aaaagattca
    22321 agcaatcact atacgcgaac taacagaaac ggcagatgta catcgttcga ctttttatac
    22381 acattataag gatatctatg atctatatga tcaattagaa agcaactttt ttcgggatct
    22441 aaatgaaatt ctctcttatg atccagccca tagctatgag gaactataca ctcgcttgat
    22501 tgactatgtt tacgcaaata cttttttata tcgcttattt atcctcaacc atggtgatat
    22561 ttcgtttcag aagaaagtta aacaagttat tgaagaaaac tatctgaaca tatggctata
    22621 tgaagacaag aaaacgatta tcacagaaga aatgagattc tttactacgt accacgtcca
    22681 aggttgtcta tccgttctca atcggtgggt agaaagcaat tacaagtact ccaaaaaaga
    22741 attattagaa ctcttacaga aactgaatga tcacatagag aaaatcatgc cttgaataaa
    22801 aacatactaa gcttagttaa gaagaaccgt aaccttctcg tataacaact gaatcttatt
    22861 tagccaaatt gtttactgaa gattttttga gtaaaatttc tataattcct acttattgaa
    22921 aagtagtttg tagtgctgct tctccctctc taagtgatat atcttgttgt accaatcaaa
    22981 ttcacctcat cattaataaa ttacttttag gctaatttat tacaacttat aggttgaagc
    23041 tgtaaccaat ttatagtcat attatatttt tatcatccag tttattgaat tcttctcgtt
    23101 tacctcaatt cattttcaag gggaatca
//
LOCUS       DACECR010000041        23006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.041, whole genome shotgun sequence.
ACCESSION   DACECR010000041 DACECR010000000
VERSION     DACECR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.041"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            46..1791
                     /gene="dnaX"
                     /locus_tag="IWO17_001456"
     CDS             46..1791
                     /gene="dnaX"
                     /locus_tag="IWO17_001456"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ0290339.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            1811..2125
                     /locus_tag="IWO17_001457"
     CDS             1811..2125
                     /locus_tag="IWO17_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ0290340.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            2150..2746
                     /gene="recR"
                     /locus_tag="IWO17_001458"
     CDS             2150..2746
                     /gene="recR"
                     /locus_tag="IWO17_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ0290341.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            2810..3454
                     /locus_tag="IWO17_001459"
     CDS             2810..3454
                     /locus_tag="IWO17_001459"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ0290342.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            3469..3798
                     /locus_tag="IWO17_001460"
     CDS             3469..3798
                     /locus_tag="IWO17_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290343.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            3812..4750
                     /gene="holB"
                     /locus_tag="IWO17_001461"
     CDS             3812..4750
                     /gene="holB"
                     /locus_tag="IWO17_001461"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ0290344.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            4786..5610
                     /locus_tag="IWO17_001462"
     CDS             4786..5610
                     /locus_tag="IWO17_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ0290345.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            5603..5950
                     /locus_tag="IWO17_001463"
     CDS             5603..5950
                     /locus_tag="IWO17_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ0290346.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            6019..6891
                     /gene="rsmI"
                     /locus_tag="IWO17_001464"
     CDS             6019..6891
                     /gene="rsmI"
                     /locus_tag="IWO17_001464"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ0290347.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            7000..8121
                     /gene="dinB"
                     /locus_tag="IWO17_001465"
     CDS             7000..8121
                     /gene="dinB"
                     /locus_tag="IWO17_001465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ0290348.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(8175..8777)
                     /gene="nrdG"
                     /locus_tag="IWO17_001466"
     CDS             complement(8175..8777)
                     /gene="nrdG"
                     /locus_tag="IWO17_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ0290349.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(9092..11281)
                     /gene="nrdD"
                     /locus_tag="IWO17_001467"
     CDS             complement(9092..11281)
                     /gene="nrdD"
                     /locus_tag="IWO17_001467"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ0290350.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            11607..12374
                     /locus_tag="IWO17_001468"
     CDS             11607..12374
                     /locus_tag="IWO17_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0290351.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            12364..14406
                     /locus_tag="IWO17_001469"
     CDS             12364..14406
                     /locus_tag="IWO17_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0290352.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            14687..14836
                     /locus_tag="IWO17_001470"
     CDS             14687..14836
                     /locus_tag="IWO17_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ0290353.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            14847..16361
                     /gene="dltA"
                     /locus_tag="IWO17_001471"
     CDS             14847..16361
                     /gene="dltA"
                     /locus_tag="IWO17_001471"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ0290354.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            16358..17563
                     /gene="dltB"
                     /locus_tag="IWO17_001472"
     CDS             16358..17563
                     /gene="dltB"
                     /locus_tag="IWO17_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ0290355.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            17608..17841
                     /gene="dltC"
                     /locus_tag="IWO17_001473"
     CDS             17608..17841
                     /gene="dltC"
                     /locus_tag="IWO17_001473"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ0290356.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            17844..19112
                     /gene="dltD"
                     /locus_tag="IWO17_001474"
     CDS             17844..19112
                     /gene="dltD"
                     /locus_tag="IWO17_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ0290357.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            19253..20335
                     /locus_tag="IWO17_001475"
     CDS             19253..20335
                     /locus_tag="IWO17_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ0290358.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            20335..21138
                     /locus_tag="IWO17_001476"
     CDS             20335..21138
                     /locus_tag="IWO17_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0290359.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            21208..21948
                     /locus_tag="IWO17_001477"
     CDS             21208..21948
                     /locus_tag="IWO17_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0290360.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            21959..22963
                     /locus_tag="IWO17_001478"
     CDS             21959..22963
                     /locus_tag="IWO17_001478"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ0290361.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT         7416 a         3951 c         4818 g         6821 t
ORIGIN      
        1 acgtataata agagatagtg aattttttaa aaaaggggga tccttatggc atatcaagca
       61 ctttatcgag tctggcgttc gcagcgtttc gaagacatcg ttggacaaaa agctgtgaca
      121 cagacactaa aaaatgctat tgtttcccac aaaacctcac atgcctactt gtttacagga
      181 cccagaggaa ccggaaagac gagtgcagca aagattttcg caaaagcaat caactgtccg
      241 aacagtaaag acggtgagcc atgcaacgaa tgcgaaatgt gtcgatcaat tactgctggt
      301 acgcaagaag atgtgatcga aatcgatgct gccagcaata acggagtcga agaaatccga
      361 tttatccgtg atcgggcaaa ttatgcgcct acaaaggcag cgtacaaagt gtacattatt
      421 gatgaggtac atatgctttc aacaggagct ttcaatgcct tgttgaaaac attggaagag
      481 ccaaaagaaa gtgtcatatt catcttagca acgaccgaac ctcacaaaat accagctacg
      541 atcatttcac ggactcagcg atttgatttt aaacggatca atacggcaga tattgtcgaa
      601 catcttgaat ttatcttgaa agaatcgact atcagctatg aaccagctgc attgaatgtg
      661 attgcccgtt ctgcagaagg cgggatgcgg gatgcattga gtattcttga tcaggcaatc
      721 tcgtttagtg aaggaacaat tactttagat gatgccatgc aagtgacagg tagcttaact
      781 gacgaaatga tggacagtta cttgagtgct tgcgtgaaaa aagaagtacc agaagcattg
      841 gaaacattga accaaattct cgcagccggc aaagaatcac gtcgctttct agaggatatg
      901 ttacaatact gccgtgactt gctgatgtat cagcaagcac ctaacctagt tgaggaacaa
      961 tcgagtacga tcagaagtac atttacagaa ttggcacaaa ctgtttcagc ggatcagctg
     1021 ttttcaatga taaagatttt gagcgatact caaaatgaaa ttcgttttac caatagtgcc
     1081 gctatttatt tggaagtcgc tacagtcaaa cttgcacagc taccgtctgc ttctgttcct
     1141 tcagcagatg tagcatttgc agatgatcag atagtaaaac agctacaaca agagattaag
     1201 gaactaagaa acgaaatcaa ggaactacgc agtaatacag gaattgagaa tacaaaagta
     1261 cagccggtac agcaaacaaa tcataaaaaa cagacaagta cctatcgtgt accgacggaa
     1321 cgagtatata aagttctgcg ggaagcgaca agacagcatc tgaaccaagt aaaagaagta
     1381 tgggaagact tgctgatgag tctatctgtc actcagcgag caatgcttaa agcaagcgaa
     1441 ccagttgcag ctggacctac aggactagtg attgcgtttg attatgagat tgtttgtcaa
     1501 agagcagcaa atgacgaaga attgcagctt tcggtacaca acaacttaag ccgaatgatc
     1561 aaagattatg cgcctgatcc tgtttatatt acaagagaaa gctggccaga acttcgaaga
     1621 aattatttga cgcaggcaca agaagaggga acagaaaact cttttgcttc tgaagactca
     1681 gaaggagaaa tcgagttgct tcctcaagaa caagaagtac cagaagaaac acttgtagta
     1741 aaagcagagg aactgtttgg ctccgaagct gttacagtgg tagatgatta ataaaaagga
     1801 gtagataatt atgatgcgtg gaatgggcaa tatgcaaggc atgatgaaac aagtacaaaa
     1861 aatgcaaaaa gaaatggcag aagcacaaga gctgctaaat aaaacagagt ttacaggtaa
     1921 agccaccaat gaattggtca aagcggtttt tacaggcgat cgtcgcatga aagatatcca
     1981 aatcaatgaa gaagtcgttg atcctgatga tatcgagatg cttcaggatc ttgtcgtaat
     2041 ggcagtcaat gatgcgatcg cacaaattga tgtggaaact gagaaaaaat tagggaaata
     2101 cgcaaaaggt ttgccaggat tttagtttta aactgaagga gtaatgaata tgcaatatcc
     2161 agaaccaatt gcgaaactga tcgacagcta catgaaactt ccaggaatcg gtcagaaaac
     2221 agcgacaaga ctcgcctttt atacgatcga tatgaaagat gaagtagtaa atgaatttgc
     2281 taaatctttg ttaagtgtaa aacgcgattt gcatttttgt agtatctgcg gaaacatcac
     2341 agaagaagat ccctgcgaaa tatgtagaaa tccttcacgt gatcgaagcg ttatcctagt
     2401 ggtcgaagag ccgaaagatg taatggcaat ggaaaaaatg cgagaatatc acggacttta
     2461 tcatgttctc catggtgtgt tatcaccgat ggaagggaca ggtccagaag acatcaatat
     2521 ttcttctctg ttgcagcggc tgcatgatgc agatgtaaaa gaagtgatta tcgcaacaaa
     2581 tgcaacaaca gagggagaag caacagctat gtatctctca cgtctgatca aacccgcagg
     2641 tatcaaagtg actcgtctag cacatggtct gtctgtaggc tctgatatcg agtatgcaga
     2701 tgaagttact ttattgaaag ctgtcgaagg aagacgtgag ttataaattt ctaattaagt
     2761 gttttttcag atacaatata aaagcagcac aagagatggg ggcaacaatg tgaacggact
     2821 atttattaca atcgaggggc ctgatggtgc aggaaaaacc agcatcttga atgaacttta
     2881 ccctcttttg aaagaagtag caaaaacaga aatcattcaa acaagagaac caggaggcat
     2941 tccgattgct gaagagatcc gagcagtgat ccttgatcca aaaaatgatc ggatggatga
     3001 acgtacggaa gcactgcttt atgcagcagc aagaagacaa catctagtcg aaaaggttct
     3061 gcctgctctg gcaaaaggga aaatcgtttt atgcgataga tttgtcgaca gttctcttgc
     3121 ttatcaaggt gcaggccgac gcatcggcgt taccgagatt gctcggttga atgagtttgc
     3181 tacagaaggt actacgccgg attttaccct ttatcttgac gtagattcag acaccggatt
     3241 aagaagaatc aagaaaaaca gacaaaatca aatcgaccgt ttagattcgg aaggtttgga
     3301 attccaccag cgtgtacgtc atgcttactt aaagctggca gaagagaatc cggaaagaat
     3361 ccacaaagtt gatgcaagaa aaagctttga agaagtgctg caaacaagtt atcatacaat
     3421 tattgaacag tatccgcaat tttttgaaaa ctaggaaggt gaacgtttat gaaaattatt
     3481 ttagcgatta tccaagacaa agacagcaac cgtttatcaa atgaattgat tgatgcaaat
     3541 atccgtgcca caaaactatc atcgacaggc ggcttcctta aggctggaaa cagtacgttt
     3601 atcgtgggta ttgaagatga ccgtgtagaa gaagcattgg aaatcatcaa aaaaacgtgt
     3661 gaatcaagaa agcaatttgt atctacacct gttacactgg atatttcgat ggatgggggc
     3721 gtcccttatc cagtagaagt agaagtaggc ggtgccacag tcttcgtctt acctgttgaa
     3781 ggattccatc aattttagga ggagctgagc tatgaatcaa gaaataaact tggctcaaat
     3841 gcaaccgatc gtgtatcagc agcttcaacg gagttttgag catgggcgta tcgctcatgc
     3901 ttatcttttt gaaggggaga aaggaacagg aaaacatgaa gtaggtatat ggcttgcaca
     3961 gcacctgttc tgtacccaaa tgaaagacaa tctgccttgt ggaaaatgca acaattgtca
     4021 gcggatcgaa aagaaagaac atccagatgt tttggttatt gaaccggaag gacaaaccat
     4081 caaagtcgat cagatcagac gtttgcagac ggaattcagt cgcagcggtt acgaatcaag
     4141 aaaaaaagtt tttttgatca aagaagctga aaaaatgaat agcagtgcgg caaacagttt
     4201 gttgaaattc ttggaagagc cccctggaga ttttttagcg gtgttagaaa cagatgcagt
     4261 aggaagaatt ctccctacta ttcaatctcg ttgtcaagtt ctgcattttc aagaactttc
     4321 gaaagaagca ctgatccatc gattgcaaga agagcagatt ccattggaaa aagcaaaact
     4381 tttggctttc ttaacaaaca gtttaggaaa agcagttgaa atctctcaag atgaatggtt
     4441 taatgatgct aaggacttga tttatcaatg gttcgtttac ttgcaaaagc aagatactca
     4501 agcctttatc tatgtccaaa aaaaattggt aaaatcattt aaagaaaaaa cacaacagtt
     4561 tacaggatta tctgtcttga tgttttatta tcaggaagca cagcgtcaag cagtagcaaa
     4621 cggcgaatat ggcaaagtga aacagataaa ccagataatt gaacggatat tgatcgcaga
     4681 gcagaaacta cgcagcaatg tcagttttca ggctgttgcg gaacagttcg ttttgcatac
     4741 gattttttag cttgccgcaa aggatgatga tcaaggagga tggaaatggt agaagtagta
     4801 ggtgtccgat atagagatgc aggtcatatc tattattttg caccaggaga ctctgaatat
     4861 gtttatagtg acaaggtatt agtcgaatct cagcaatcct tacatattgc tactgttgtc
     4921 cttcctaaaa aagagattga tcccaatgac cttcctgaag ctatccgtcc gatcttgcat
     4981 aaagcgtcac aagctgaatt agaaaaagac aaaaaaaaca aaaaagatgc ttatgatgca
     5041 ttgaaaatcg caaaagaaaa agtgcagttg cgtgagttgg aaatgaaaat ggtcaaagcc
     5101 gaatacacat ttgatcgaaa taaattgatt ttttctttca cagcggacgg gcgaattgac
     5161 tttagagaat tggtaaaaga tctggccgct gtatttcgta ctcgtattga gcttcgacaa
     5221 attggtgtcc gcgatgaagc aaagataata ggcggaattg gtccatgtgg gcgtccatta
     5281 tgctgttctt cttttttagg cgatttcgtg cctgtatcaa tcaaaatggc aaaagatcaa
     5341 agtttatctt tgaatccaac aaaaatttcc ggtttatgcg gacgcttgat gtgctgtctg
     5401 caatatgaga gcgaagaata cgagacagcg aaaaaagaac tccctgactt tggaaaagta
     5461 attgctacac ctgatggtaa agggaaagtc gtagggttga atctgcttag ccgtatcgtt
     5521 agtattcggc tgactggacg ggaaaatcct gtcgaatatg aatgggatga attgaaagaa
     5581 tttatccaag taggtgagat gaatggataa gagatcatta tacgacggat tgaatcaatt
     5641 agaatcagag ttgagaaata cattaacaga acttgttgaa atgaagcatg ctttgcatga
     5701 gatcgtagaa aaaaatacaa ctttggagat ggaaaatcaa cgtctgcggg aacacctgcg
     5761 ggaattgaac caagttgcac aagctccagc tgaaaatgtg aagcaggaac tttctaagtc
     5821 tcgtatgaat cttgaaaaaa tttatgaaga aggattccat gtctgctacg atttatatgg
     5881 atcaagaagg gacaatgacg agccttgtgc gttttgtcta gaagtgatct atcgcgaaag
     5941 cggtcgatga ccaataacaa gaggagactg gagcggagaa atactgctcc agtcttttta
     6001 ttaaggagat tgtgacagat gtataatcaa aaaagtttca agggaaatcc ggtatatggg
     6061 gatttatatc ttgtaccgac gccaattgga aatttagaag atatgagcta cagaagtgtt
     6121 cgtatgctgc aagaatccga tcttatcgca agtgaggata cgcgtaatac acaaaagtta
     6181 ttgaatcatt ttgaaatcca tacaccccaa aaaagtttac atgaacataa ttataaagaa
     6241 cgtgtccctc aattgattgc tttgttaaaa gaaggaaaaa cgattgctca agtcagtgac
     6301 gcaggaatgc cttccatcag cgatcctgga catgagctag ttttagcctg cataaaagaa
     6361 gggatttctg tgatctctat cccaggacct acagctggga tgacggcttt gattgcttca
     6421 gggctagtgc ctcaaccatt tttattttat ggcttccttc cacgtaagaa aaaagaacag
     6481 aaagaaacat tgaaaaagct aaaagaacag acagttacgc tgatttttta tgagtcacct
     6541 tatcggatca ctgctacatt gacgaatttc ttagaaattt ttggaaatcg ccaagtcgtt
     6601 ttgtgtcggg aattgacaaa ggtccatgaa gaatacctta gaggaagtac ggaagagcta
     6661 ctcgcttata tcgaagaaca tccagtaaag ggtgaatgtt gtctgcttgt agaagggaat
     6721 ttgttcgaaa tgccagtgac ggaagttgat gaagagttgg gaacgttaaa ggaacaagta
     6781 gaagcaaaga tcgcttcagg cgaaaaacca aatgctgcta tcaaagcagt agcgctgaaa
     6841 aatgggctga aaaaacaaga agtctaccgt cagtaccatg aattagactg agatagtata
     6901 agttgtctat aatgaaagac taatgaggaa atcagctact aacttttcaa atctcttgaa
     6961 cgcgaacgaa tgttcttgta taatgaataa gaggtgagga tgatggagct gcaatttcct
     7021 ttacataatg atacatcaag aaaaatcatt catattgata tggatgcttt tttcgcttcg
     7081 gttgaagaac gagatcatcc tgagtttcgc ggacatccat tagtaattgc cagacacccc
     7141 agtgacactg gtgggcgtgg agtagtgact acagcaaatt atgaagcaag aaaattcgga
     7201 attcattcag caatgagtgc acaaaaagcg tatgagcttt gtccgcaagc catctttaag
     7261 cctggcgacc atcataaata cagcgatatt tccaaacaag tacgggaagt attctatcgt
     7321 tatacagata ttgtggaacc agttagtatc gatgaagcgt atcttgatgt gaccgaaaat
     7381 aaaatcaatt gcaagtcagc catcaaaatc gcccgaatga tccaagcaga tatttgggag
     7441 agtgtccagc taacatgttc tgcgggagta agttacaata agtttttggc caaattagca
     7501 tcagactatc aaaaacctag aggaatcacg gtagttgccc ctcaagacgc tgtgccattt
     7561 ttaaaagctt tgccaattga aaaatttcat ggggtaggga agaaaactgt acctagaatg
     7621 catgagctag ggatttatac aggaaaagac ctatatgaat gcacagaaat gatgttgatt
     7681 cgaaattttg gaaaaatggg ctattctctt tatcgtaaag tcagaggaat ccatgattct
     7741 ccagtaaacg ttacaagaga gcgaaaatct gtgggaaaag agcatactta tgggcagccg
     7801 ttgcaaacgg aagaagcagt gctgactcag ttgcgccaac tagctgaaaa agtagaagaa
     7861 gcactgagaa gagtccaaaa acatgggaaa acagtagttt tgaaggtcag gtacacggac
     7921 tattctactg tgaccaaaag agtgacattg ccagaatata tctataaaaa agaagcactc
     7981 ttttatcaag caagccttat atgggaggaa atcttgggag tagaaaaagg aatccgattg
     8041 ttagggatta ctttgacgaa tctggacccg atgacttatg aaaacatcgt ccttccatta
     8101 tgggaaaatc aagaaatata aaaacagctt tgagagttga aaccggaaat ccgttttctc
     8161 tcccaaagct gtttttatat caagtcttgc tttttgattt gttcataggt ttctgttgca
     8221 tccgtacatt tttcccaaat cacaacttga ttggattcta aagatttttg gacgtcaatg
     8281 atccgctggt tgctacttcc gcgaaattgt agttttaggt cacgtttgct taattcaaag
     8341 cgtccgtcaa caaggatatc gatttgcgaa aggagttcta atttatcttc tgaatcttgt
     8401 agcaattcat caaaggtata gcctgtccat gaccagatat cttttgtcgt accgaactct
     8461 tttcggatac gtcgtacgac ttgcagacaa acatctgtat tcaaaaaagg ttcgccacca
     8521 agtaaagtta atccttgcac gtaatcgttg cgcaagtcag cgatgatttg ttcttctaat
     8581 tcaggtgtga atggtttgcc atatcggaaa ttttgtgcag ctttgttgaa gcagccttcg
     8641 caggcaaata agcagccact aacgtaaaga ctgttgcgga caccttcacc atcgacaaaa
     8701 ttaaacggtt tgtagtcagc aatatagttt tggctgtagt cttttgctaa ccattctttt
     8761 ggttttggat ttctcataaa aaaacttctt tcatttaagg ttcaaagata tagctattga
     8821 agataaaatc tactcgacca cataaataag aacaaactga cctaaatcca ttgttcaaga
     8881 aagcttgttc ttattttgat agagggcgtt caaagataca aagccatcga aagagggtgg
     8941 tgaaaaaaga cttgtcatag cctcttattc tgaataaacg gtgttcaaaa gcttgcatct
     9001 gcagtaataa gcccaactaa acgaaaaata tgaggaacta tttttgttta gctattctta
     9061 ttacttgatg caggacagct ttttcacaac ctcatttcat gtgtttcaca cgtgaggaaa
     9121 tttctttatg gcggccatgt accatcggac gagcttgcgg atttcctaaa tagccacaag
     9181 tacgtttgac tacatcacat gtttttggat catgatttcc acattctgga cattcgaacc
     9241 ctcgttctgt cggtttgaaa tcaccttcga aaccacattg atagcaatga tcgattggcg
     9301 tattcgtacc aagatacccg attttgtcat atgcaaaatc ccatacagct tctaaagctt
     9361 tcggattttg ttggagcata gggtattcac agtagtggat gaatccacct gaacaatatt
     9421 ttggataatc cttctcaaat tccaattttt caaatggcgt tgggttttta cgcacatcat
     9481 agtggaaact gtttgtataa tattctttat ctgtgatatt ttcgatgatg ccgaattttt
     9541 ctgtatccaa acggcagaaa cggtcagtta gactttcact tggagttgaa tagacactga
     9601 aatgataacc atattcatcc ccccatgcat ctgcattagc ttttaattct ttgacgatat
     9661 caagtgtgaa agcttttgct tctgggtttg tttcccattc gccaccgaag aaagctgaag
     9721 caacttcata caatccaatg tagcctaacg atacagtagc tcgacggttt ttgaataatt
     9781 cattgacaga atcggtacgt gaaagacgtt tcccgaaagc accatacata tataaaattg
     9841 gcgcatttgc tggaatggct tctttacaac gttctacacg atagagaagc gcatctttca
     9901 tgatttctaa gcggtcagac agtaactgcc agaatttatt ctgatctcct tttgattcga
     9961 tcgcgattct tggcaaattc aatgtaacaa cacctaaatt cattcgtccg acatttactt
    10021 cttgaccatt ttcgtctttc cacccttgca agaatgaacg acagcccata ggtactttga
    10081 aacttccagt taattcaacg attttatcgt aattcagaat atcaggatac attcttttcg
    10141 ttgcacattc caatgctagt tgtttgatgt cgtaattagg atcttcaggg tttaagttca
    10201 cacctttttt caaagtgaag atcagtttag ggaagatagc tgtccgcttt tcactcccta
    10261 atccattgat tcgaatctgt aaaattgctt tttgaatttc tctttcaaac cagttttctc
    10321 ctagtccaaa tcctaatgaa gtgaaaggag tttgtccatt tgaagtaaat aaggtattga
    10381 tttcatattc caaactttgc atagcatcga agatatcttt tttggttttt gcttttgcat
    10441 attctttttg gcgttctggc gaatcgatcc attcttccgc gtcttttaga tgttttttat
    10501 agttcaattc tgcaaaagga gcaagcaatt catctgttcg gtcggcagaa cagccaccat
    10561 attgacttga cgctacattg gcaataatct gtgagatttg tgctgtggcg gtctggatag
    10621 attttggcga ttcgacttcg gcattgccga ttttgaaccc attgttcagc atgcctttga
    10681 aatcaatcaa gcagcaattc gtcatcggtg tataaggatg ataatccaaa tcatggtaat
    10741 ggatatcgcc tttttgatgc gcatttgcta tatgtggtgg caacattttc agtccgattg
    10801 attttccaac gatcccggct gtcagatctc gctgtgtatt gaaaacatta ctgtctttat
    10861 ttgcgttttc gttcacgaca gtttggtcct tgttgattag cttttcaatc gaaaagttga
    10921 tatctgttgc tttactgcga gcaaaatctc gtttggtacg ataattgatg tactcttctg
    10981 ctaattcata ttcgtttttt tctagtaata tatgttccac gatgttttga atctcataga
    11041 ttttgacatt ttcagcaaac cggcttttga tttcttggac aaccaagtct gcaatcattt
    11101 gaattttttc ataagtaagt ggtgataatg agccgtgaat tttcttttct gctttgatca
    11161 gtgcttcatt gatcttttgt tcatcgaaat caacatggcg gccatcgcgt ttgatgactt
    11221 tcatctggga tgtctctggc tgctgagtaa cgacagtttt ttcttcataa tttactttca
    11281 ttatcttcaa ctcctgctaa attgttcttg tctatcataa aacaaaatcg gggaaggagc
    11341 aactaaatat cggctatata tagtattcaa tgtgctgtat aattggattt ctatactata
    11401 tgtagtgtat attttattgt gaaaaaaggt gaaaacttgt agctggtgga tttgtttttt
    11461 gcatatttaa ttttgctgaa taaaataatt gaataaaatt ttttattcag tccaagtaaa
    11521 agtgacaaaa acataagagc aaattattcg tagatatgct actatgcaaa taaatagaaa
    11581 tgaaaaatga aagaggagga gaagaattgg aaaagatgat cgaagtgaaa catctaagta
    11641 aaagctacgg cagcaaactt tctcccgtca aagccttaga taatatctca ttcacagtag
    11701 aagaaggcga gtttgttggg attatgggcc caagtggtgc aggaaagaca acgctgatga
    11761 atatcttgtc cacgatcaat ttgcctacga tgggcagtgt ctcgatccaa ggaaaagaaa
    11821 tcacaaaaat gaaaaacagt gagctcagtg actttcgtcg aaaaaaacta ggattcattt
    11881 ttcaagagtt caatttgatc gataccctaa atgcaaaaga caatatccta ttgcccctag
    11941 cagttgaacg gatgactaaa gaggaaatgg aaaagagagt aagacatgta gctcaattgt
    12001 tgaatataga agaactgctc aaacggtatc ccgatgaact ttctgtcgga cagagacaac
    12061 gaattgcagc tgctagagca cttgttgttc agccacaagt gatttttgca gatgaaccaa
    12121 ctggctcttt ggactcgaag tcggctacag aattgttgaa ctacttgaag accatgaatc
    12181 aaaaagaaaa agcaacgata ctgttagtca cccatgatcc atatacagca agctactgcg
    12241 atagaatttt atttattaaa gatggtgtga ttttttctga agttgtcaga agagggacaa
    12301 gaagagagtt ctttgagaaa gtaatagaca tgcaggcgac gattggcgga ggaggaaaaa
    12361 tgaatgctgt ataaactagc cgttaaaagt ttttttcggc agtcacgagg ctatcttgtt
    12421 tattttttca gtttgacttt atccgtaatg atctattatt ccttcagcgc aatgacctac
    12481 gatcaatctt tagtcagaag agcaagtcaa gatacttcga tagatggtgg attgagtttt
    12541 ggtggattta tcattactgt tgttttgctt tttttcgtat tcagtgccaa ccgatttttc
    12601 ttgaatagaa ggcaaaagga aataggcatc tatcagcttt ttggtatgaa taaactgcag
    12661 atatcaggta tatatgtgtt agaaatcatg ataattggat tgttcgcctg tatttctggg
    12721 atattactcg gtattatttt ttccaaactc ttttcaatga ttttggttcg aatgatggat
    12781 atggatctta ctagtccatt ttttatctcc attccatcag tagtagatac tttatttgct
    12841 tttttcatca tccttttagc agtatctgta tattcaatat ggaaaatatg gcgctatcca
    12901 atgattcgaa gttttggaga aaaagaacaa gcagatagtt caacaatgcg cttcaggaca
    12961 aggcaccgat tactcgcagt catcgggtta ctacttttaa cgagcggata ttttggagct
    13021 agtcatttta gggaaatcac ttcatggatg atcagttatc attatcttga tacattagtt
    13081 ttcttttttc cttttttaat ttttttcgtt tgtgtggtcg gtacttattt gtttttctgt
    13141 tatacatttc ggttattttt gatcatattg agtaaatgga agatttatta ctatggtatc
    13201 aattttttga taatgggtaa cacgcaagtt catttgctca aaagttggcg tacaaattct
    13261 ctgattacag tcattctcgg aatttcttta atgacaattg gcggcacggc tagtatctct
    13321 actattttat cctaccagga gaaagtttcc tccccgatgg actatcagtt tgatgttgag
    13381 acagagaaaa aaatcaagcc gattctagca gaggaagggc agaagataac taaagaaatc
    13441 accttgcatt ataaagtcat tgggagtatc tatagtcaaa atctcgaaga acctatgttt
    13501 caaactgcta atctgatcac ggaaacacaa tatcaagcct tcagaaaagt taatccagat
    13561 ttgccgaaca tttctttgaa atcacctggt catactgttt tgcttgatca aatgcaaact
    13621 ctttttcgga atatttcatt atatggatct agtattggac tgccagataa tcaagaattg
    13681 aagattcaac aaatgcttcc aagtgtatta ggtgacgaaa caatggtata tctaagtcct
    13741 gtactgattg tagatgatac cgtttttgaa aaagcaaaag gtgtggatta tcaagtagtc
    13801 aatgtagatg tatcaggtgg aaataatgaa aaaatagatg aacgactgag caatgacctg
    13861 tcgataaagt ggctcaatgc tgtttattat tcttatgata tcgtaaatgg aagtctacaa
    13921 ggagaaatta gcacaaagaa acttccggat gaccaaatga aggaagcaca atttagccaa
    13981 gaaagctctc gtttgaattt ctcttccagc tattcgaaat tgcgtgcagt taatcggcga
    14041 atcggtatca atatttttgt cacactattt gtcgggatga tcgtgattat tgctactggt
    14101 agtattttga cagtccggca attagcagaa gcagaggaag aaaaagaaaa ttatcaccta
    14161 ttgactaagc tggggattcc acaaagaagg atcagacgga tgatcttcga gcaaaacgca
    14221 cttaccttct ttccacccat gatattaggg atcactcacg cagtatttgc aatcaatgtc
    14281 ttcactcagt atataaaaac tgccgattac tggttggctt atcttgtgag tggcttattg
    14341 atccttatct atcttttgct ttatgtcatt acgagtcagt tatattgtcg gattatcgag
    14401 gaatgatata cggaataaga caagataata aatagaacta aattttgaat accatttatt
    14461 tggaataaga ggttgtgaca cgatttttgt cacaacctct ttatttttta tattttttaa
    14521 aagtacgaga aattataaag aaaaaagcag tgatttgatg tgctattttt ataaaagcta
    14581 tgtgacaaaa ctgttagtaa aaaaacggtt tctgtaagct agacacatgt ttctttttgt
    14641 caaaattagt tacactttgt gtgaggttat atgaggaggg aatccaatga acaaatggcg
    14701 ttctaatcca aaattgaatt attggggaaa attcatcatt cgaacaattt tttatagtgc
    14761 agtactgctg ttcctgattt atctgtatca ctataaaaat attcaaggag gaacttttat
    14821 ctataacgaa ttttaggagg aagagtatgg aaatcaaaac gattattgaa gcaatcgatg
    14881 attggggaat aaatgaacct gatcgagttg cttatcaggc ggatgagacg catacttttg
    14941 gtgaattgaa acaagcatca gacgcacttg cttattattt agaaaaaaag gtagaaggcg
    15001 atggaccaat tgtcgttttt ggcaatttgg agtttgaaat gattgtctca tttctagggg
    15061 ttgtcaaatc tggccatgcc tatttgccga ttgaagagca tacacccaag gagcgtatcc
    15121 tatcgatctt tcgggtggct aaaccatcta tggtcatcag tatcggggac tggatagaag
    15181 atattccttc ggttcctgtc ataacaaaaa aagaatttca agaaatcaag aagataccag
    15241 taggatttta tcccagaaat agcgtaaaag gtacagaaaa tttttatatt atttttactt
    15301 ctggtactac tggagaacca aaaggcgtac aaatcagtca tgataatcta gtcagctttg
    15361 ttcaatggac gatggaagac tttgggatca agcctggtat gcatttctta gcccaagcgc
    15421 cattttcttt tgatttatcg gtattttcta tttatcctgc attagtttct ggcggaatgc
    15481 tgaagccgtt aaataaagca gttgttcaag actttcgtca attattcgcg acattgccag
    15541 ccttgaagtt gaatgtgtgg gtttctactc catcatttat ggatatttgc ttgatggaac
    15601 ctacattcaa tggagaaaat gtgccggaat tggacatgtt tttattttgc ggagaagaat
    15661 tgacgaaaaa aactgcagaa agtctacttg atcgattccc aaatgcacgg atatataata
    15721 cttacggtcc aacagaagca actgtagcta tttcaagtat tcagattgac cagcatgtac
    15781 tagatagtta tgaccgacta ccgattggct acgtaaaaaa agacacgaaa gtttctattg
    15841 tccaagaagg taaacctgtt tcaaaaggag aaacgggtga aatcattatt gcaggaccaa
    15901 gcgtatcaaa aggttacctg aacaatcccg aaaagacagc tgctgcgttc ttttgtcaaa
    15961 atgacttagc aagttaccat acaggggatg caggtagact agatgaatat gggcttttat
    16021 tttatgaagg acgtatggat cagcaggtca aattacatgg ataccggatt gaactcgggg
    16081 atatcgaaca ttatctgctc caagacaacc gaatcaaaca agctgtcgtc gtaccaaaat
    16141 accaaggaac aaaagtacag cagctggttg cttttgtcgt attagaaaac aaaacgaaac
    16201 agccggattt tcagttgact aaatctatca aagaacaatt actagaagtt gtaatggatt
    16261 atatgattcc tcagaagttc cattatgtcg atctgctgcc tcagacagtt aatggaaaaa
    16321 tcgatcgaaa aaaattaaca gctgaggtga atcctgaatg atgttacctc atatgatccc
    16381 ttatgcagag ccgttttatt tttttctttt agccattgcg cttgtaccga tcgttctatc
    16441 tttattgata tgggaaaaaa gattaccagt ttatcaatca gcagtgacac ttttcttttt
    16501 atttgtcagc tttggaggta attattggca acagggagcg gcgctgattg ggtatgtttt
    16561 gtggcagacg atacttgtat ggggctattt tgtttatcgg caaaggaaaa atcagacagg
    16621 atggtttttt gccgccgtta ttttagcgat aattccactg tttttaacga aagtgactcc
    16681 tttttttgaa catggaaaag catcgttact tggtttttta ggaatctctt atttaacctt
    16741 taaatctgta caaatgatca tggaaataag agatggcatg atcaaaacat ataagccatt
    16801 tcgttatatc cagtttctgc tgttctttcc aacaatctct tctggaccaa tcgatcgcta
    16861 taggagattt gaaaaagatc agctccatcc gcctgttcgt gataaatatg ttgatctttt
    16921 gggaaaaggc gtccaaaata tttttgtcgg ttttttatat aaattcatta tcggttatta
    16981 ttttggtcaa gtactgcttc ctgtagtggg acgcattgcc ttacagcatg gtggtatttc
    17041 atgggcatta gtaggttata tgtacgtgta cagtatgtat ttgttttttg attttgctgg
    17101 ctatagctta tttgcagtcg gtacaagcta catgatggga tatcagacac caatcaattt
    17161 caataaacct tttttatcat ggaatatcaa agaattctgg aatcgttggc atatgacgct
    17221 gtccttttgg ttccgtgatt atgtatatat gcgattgatg ttcttcatga tgaagaaaaa
    17281 attttttaaa agcaagatcg taatgtcaaa tatcggttat tttgctttgt ttcttctaat
    17341 gggtatatgg catggattga cgtggtacta catcgtatat ggactgtatc atgcttgtct
    17401 gatctgtctg acagatgcat ggatccgatt taagaagaag cataaagagc agttgccttc
    17461 taacaaattt acgcatggct tagccgtatt tttgacattt caagcagtat gttttagctt
    17521 cctgattttt tcaggatttt tagataaact tttttttagc taacgaatag tgacatatta
    17581 atttatataa caaaggagaa aaggaaaatg gaagaacaaa ttttatcaat cttagaagaa
    17641 ataacaggaa cagatgaagt aagaaaaaat cgtgatattg atctatttga tgaagggttg
    17701 cttgattctt tagcatctgt tcaattatta gtcgagctag acggagagct ggatatccaa
    17761 gtccctgtat ctgaattcga gcgagaggat tggagcacac cgaataaaat cattcaacaa
    17821 gcaactgcat tgaagggttg agtatgaaaa agaaactttt tgctattttc ggcccggttg
    17881 ttttagctgt tggcttactg tttgtgtttt ttacctcctc ctatcaagta gatatctcta
    17941 atccagaaac gattagacgt gcctcttctt ccatgtcagt gaacgtgttg aaaggagatg
    18001 cgataaaaaa ttatgcatta tctgaaaaac aatatattcc tttttttggc tcaagtgaat
    18061 taagcagaat cagtccattt catccgtctg ttctggcaga aaaataccag cgaaactatc
    18121 gtccattttt gctgggagca cccgggacac aatcgttaag ccaatatatg atgatgcgtt
    18181 ctgcaggaga tgctatgaaa aacaagaaag tcgtctttat tatttctcct cagtggttcg
    18241 ttaaaaatgg tgtaaaaact gattacttta atacgtatta ttctgaactt caaacatacg
    18301 actggttgtt ttcaatgaag aaagtaacgc ctgctgatcg ctatttagct agaagattat
    18361 taactttctc aaaagtaaaa gagaatgata cgttgacggc gattctgcaa acgatcaaaa
    18421 aagggaagct acctttacct gaatctttga atcagctgcg cagccaatgg aacatgctga
    18481 agagagagga tgaagtattc agcaatattg ggttgaaaga tcgacagcaa aaaatcgatc
    18541 atgagtcaaa acgattacca aaacaatacc aagaaacaga attatccatt ttagcaaatc
    18601 aaattggaga acgagaaacg acgaataatc cattcgggtt gaaaaacgat ttttatacgc
    18661 atcgtatccg agcccacgag ccagagctca aacagtcaca aaaaaattgg gattatcgtt
    18721 tttcgccaga attttctgat tttcagctgg tcttggatca attagcaaaa aaccataacg
    18781 aagtattgtt catcatccca ccggttaacg agaaatggag cgattatacc ggactatctc
    18841 aagaaatgtt acaaggattt gccaaaaaga tcaaatttca attaaatagc caaggattca
    18901 atcgaatagc tgattttgtc aatcaagcgg gtaccaacta ttttatggaa gacacgatcc
    18961 atctaggctg gaaaggctgg ttggctgcag atcaacagat cagaccgttt ttagaagaaa
    19021 atcatattac tgctagtaag tatcatcttg acgatgcttt ttttagcaaa tcttggcaac
    19081 atcaaatacc agataaacta caattaaaat aaagaaaaca ggcgagtgtt gctcgcttgt
    19141 tttctttatt tttgaatatt ctgaataaaa gataaaaata ccaagtttgc ctgaacaaaa
    19201 atcataagca atggtataat cacaatatga atatagaaat gggggaaaac atttggatac
    19261 aaaagaagaa cagctactta ttgatttaac ttctgcaaaa ggagtacctg gaaatgaaga
    19321 agaggtaaga gaagtattca gagaatatgc caaaccattc gctgatgata ttttttatga
    19381 cggtttaggc agtgtgattg ccaaacatgg tgctggcgga ggccccaagg tctttatttc
    19441 tggacatatg gacgaagttg gttttatggt aacaaaaata accgaaaaag gattcttgga
    19501 attccaaact cttggaggtt ggtgggggca agtcatgctt gctcagcaag ttgaaatcaa
    19561 aacacgtgaa gggaaaatcg tccacggtgt gattggttcc aaaccaccac atgttttaac
    19621 acctcaagta agaaataaac cttatgaaat aaaggatatg ttcattgata ttggcgcatc
    19681 aagccaagaa gaagccaaag agtggggcat acgtcctgga gatatggtca ctccatatat
    19741 tgaatacaaa cgaatgaatg gcagtaaata tttactggca aaagcatggg ataatcgtat
    19801 cgggacagct gtttctctgc gagtattaga gaatctatct aaagaagcac acccaaatgt
    19861 tttattcgca ggaagcgatg tgcaagaaga agtaggcttg cgaggtgctc gaacaagtac
    19921 acaccttgta aatcccgata ttgcttttgc tttagatact ggtacagcag gagacactcc
    19981 aggaatgaca ccaaaagaag cagattcgat tttaggaaaa ggcccacaga ttttgatttt
    20041 tgatgcatct atgatcccgc ataaaaaatt attgaatttt gttattgatg tagcagagga
    20101 aatggatatt ccattccaat atacagtgat ttctggcgga ggaactgatg caggacaaat
    20161 gcatttgaca agagatggtg ttccgtctgt agccattacc gttcctgttc gttatctgca
    20221 ttcccataca tcagtgattc atgaagatga ttatttgaat acagtgaaac tagtcacaga
    20281 attagtgaaa cggcttgata gtgaaacagt tgaaaaaatt cggagttatg aataatgaat
    20341 aatagaatac ctatttctga atatcggaaa aaagcacgag tttctttgga aaatcaatgg
    20401 ggaatcaata cttggcttgt tttcttatct ataatattta cgagcgtcat ccagaacttt
    20461 tttgacggtt tcagtccttt tccggaagaa agcatgcagc agcacttatt gagttttcta
    20521 gtcgataatt tacttttatt cgcattcaac tatgcattgt tttacattgc gctagtagtt
    20581 gtacggggtg gcagaccaaa aagtaacctt ttattttcag ttttccaaag aaagtatttt
    20641 ggttcgatac ttggcatcaa tttgatcaat tcagtcatca actggctact caacagttta
    20701 atctttctgc ccatgttttt aatcagcgga gtgagtactt atatgcagct gcttttcagt
    20761 tcaaatgcgg tacctacggg tgtcatgcca gaagattttc ttgatatcag ctttgtttta
    20821 tcgatgatag ggatgatttt tgtttcagca ctcatcatga gtattgttgg cggtttattc
    20881 caatttgctg catggacaaa gttcgactat cctgatttgt cggttattca atgtttgaaa
    20941 tatggctggt ttttattgaa agatagaatt ggtacgtata ttttgttgca gctgtctttt
    21001 atcggctggt atttgctagg agcaatcgct ttgttcatcg gtcttttgtg ggtcattgtt
    21061 tatgtgaatg tgtcagttgc agaattttat gaacaagcac ggattgaaaa aggaggtcca
    21121 gacgcctatt ttacttagca tgtaagcagt ttattcatca aaaaaataac ttttgtggta
    21181 ggataaatct ttgaggggga atgtacgatg aaattaactg ttttaggatg cttaggagcg
    21241 tatccttata aaaatcaagg gacaacaggt tatttgcttc aatctgacgg gttcaatttg
    21301 ttgatcgatg caggtagtgc tacgctgatc aaattacaag aacaccttga tccacttgat
    21361 ctagatgctg tgatccttag ccattatcat catgatcata ttgctgacct tggtgtatta
    21421 cagtattact ggcaacttca tcctaagaga aatccggaga cattgctttc aatttatgga
    21481 cacacaaaag attccattca ctttgaagaa ttgacgttgg aaaatgtctc aaaaggacat
    21541 ccttattttg aagctgaaga attaaaaatt ggtccttttc tgattacttt tatggaaaca
    21601 atccatccag tagtatgtta cgccatgcgt atcgtagaag aaacaacggg gaaagtattc
    21661 gtatttacag gtgattcagg ttatttggaa agtttcgctg attttgcgaa agatgctgat
    21721 ttgtttctag cagataccta tctttttgaa ggaaatgaac atcatcatgc ccatttcact
    21781 tcaaaagaag ctggagaact tgcgaaatca gctaacgtaa agaaacttgt acttacacac
    21841 ctacctcagt ttggtgattt agaacaattg aaaaatgaag cgcaaaaggc agcaggcaaa
    21901 gggattgata tagaacttgc tgcagttgac aaagtgttta acatttagga ggaagaacat
    21961 gatttttgta ccaaatgaaa accacgaccc aagaatcaat ttagcaattg aacaatattt
    22021 gctgcaagaa atgcctttag atgaacctat tttgctcttt tatatcaatg aaccttcaat
    22081 catcatcggc agaaaccaaa atacgcttga agaaatcaat cgagattatg tggaagaaaa
    22141 tggcattcat gttgtccgtc gcttaagtgg aggcggagct gtctaccatg attttggaaa
    22201 cctgaacttt agttttatca tgcctgatga tggggattca ttccgtgact ttgcgaaagt
    22261 aacacagcca atcatccaag cacttcatga attgggtgtt gctggggcgg aattgaaagg
    22321 ccgaaatgat ctagtcattg atggcatgaa attttctggc aacgcgatgt atgctaccaa
    22381 cggtcggatg tttgctcatg gaacaattat gttcgactct gatatcaatg aagtagtcaa
    22441 tgcattaaaa gtaaaaaaag acaaaatcga atcaaaagga atcaaatcga tccgttctcg
    22501 tgtaacgaat atcaaaccat ttcttccaga agaaaagcaa aaaatgacaa cagaagaatt
    22561 tcgtcaagaa atcttgttga aaattttcgg cgtagagtca gtagaagaag taaaaacgta
    22621 cgaattgact gatgaagatt ggaaaaaaat caatcgaatc tctgaggaat attatcgaaa
    22681 ttgggattgg aattacggga agtctccaga tttcaatttc tctcgccaaa aacgtttttc
    22741 tatcggttcg attgaagtcc atttgaatgt ctcagaagga atgatcaaag aagcaaaaat
    22801 ctttggcgat ttcttcggac taggagatat ctctgacgtg gaaaaaaaat tggttggaca
    22861 gaaatatgat aaagcatctc tatcaaaaat agtcagccaa attgatgtga agaaatattt
    22921 tggtgcgata gaaccagaag aattgcttac cttactttat tgaaaataaa aaatagcgga
    22981 atccacatat atggattccg ctattt
//
LOCUS       DACECR010000042        22984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.042, whole genome shotgun sequence.
ACCESSION   DACECR010000042 DACECR010000000
VERSION     DACECR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.042"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(108..563)
                     /locus_tag="IWO17_001479"
     CDS             complement(108..563)
                     /locus_tag="IWO17_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290362.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(717..1925)
                     /locus_tag="IWO17_001480"
     CDS             complement(717..1925)
                     /locus_tag="IWO17_001480"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ0290363.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2037..2369)
                     /locus_tag="IWO17_001481"
     CDS             complement(2037..2369)
                     /locus_tag="IWO17_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0290364.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2517..3392
                     /locus_tag="IWO17_001482"
     CDS             2517..3392
                     /locus_tag="IWO17_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0290365.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3474..4055)
                     /locus_tag="IWO17_001483"
     CDS             complement(3474..4055)
                     /locus_tag="IWO17_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ0290366.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4061..5320)
                     /locus_tag="IWO17_001484"
     CDS             complement(4061..5320)
                     /locus_tag="IWO17_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0290367.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5322..6368)
                     /locus_tag="IWO17_001485"
     CDS             complement(5322..6368)
                     /locus_tag="IWO17_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290368.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6603..6878)
                     /locus_tag="IWO17_001486"
     CDS             complement(6603..6878)
                     /locus_tag="IWO17_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ0290369.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7121..8431)
                     /locus_tag="IWO17_001487"
     CDS             complement(7121..8431)
                     /locus_tag="IWO17_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ0290370.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8613..9842)
                     /gene="rpsA"
                     /locus_tag="IWO17_001488"
     CDS             complement(8613..9842)
                     /gene="rpsA"
                     /locus_tag="IWO17_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ0290371.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9977..10657)
                     /locus_tag="IWO17_001489"
     CDS             complement(9977..10657)
                     /locus_tag="IWO17_001489"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ0290372.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10706..11341)
                     /locus_tag="IWO17_001490"
     CDS             complement(10706..11341)
                     /locus_tag="IWO17_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0290373.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11407..12810)
                     /locus_tag="IWO17_001491"
     CDS             complement(11407..12810)
                     /locus_tag="IWO17_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ0290374.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12797..13828)
                     /locus_tag="IWO17_001492"
     CDS             complement(12797..13828)
                     /locus_tag="IWO17_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290375.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13859..14080
                     /locus_tag="IWO17_001493"
     CDS             13859..14080
                     /locus_tag="IWO17_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ0290376.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14245..14982)
                     /locus_tag="IWO17_001494"
     CDS             complement(14245..14982)
                     /locus_tag="IWO17_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ0290377.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15135..16475)
                     /locus_tag="IWO17_001495"
     CDS             complement(15135..16475)
                     /locus_tag="IWO17_001495"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ0290378.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16712..18235)
                     /locus_tag="IWO17_001496"
     CDS             complement(16712..18235)
                     /locus_tag="IWO17_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ0290379.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18613..19224)
                     /locus_tag="IWO17_001497"
     CDS             complement(18613..19224)
                     /locus_tag="IWO17_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0290380.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19347..19472)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19554..20270)
                     /locus_tag="IWO17_001498"
     CDS             complement(19554..20270)
                     /locus_tag="IWO17_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0290381.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20276..20845)
                     /gene="scpB"
                     /locus_tag="IWO17_001499"
     CDS             complement(20276..20845)
                     /gene="scpB"
                     /locus_tag="IWO17_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ0290382.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20829..21626)
                     /locus_tag="IWO17_001500"
     CDS             complement(20829..21626)
                     /locus_tag="IWO17_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ0290383.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21596..21967)
                     /locus_tag="IWO17_001501"
     CDS             complement(21596..21967)
                     /locus_tag="IWO17_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0290384.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21986..22873)
                     /gene="xerD"
                     /locus_tag="IWO17_001502"
     CDS             complement(21986..22873)
                     /gene="xerD"
                     /locus_tag="IWO17_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ0290385.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6444 a         4912 c         3900 g         7728 t
ORIGIN      
        1 aatcttgtca cagtccctta ttctgaataa acggtgtgga agagacagcc tatcagtagt
       61 aataagacac atctctttca cagcctcatt tttgcgtaaa aatcgattta ttcgaaaaat
      121 tccccgtata catcttctac acgttgatct ggattgatca cgatatacaa gttacctgtt
      181 ttttcgctga tttttgttga ttgatattga ttgtctaatc cttctgttcc tttatctaga
      241 tatgggaagt acacaagatc aggtgtgcca tttcggtaaa ggatttccat tcgctcgata
      301 tcttcgtatt tcaatgcgcg attaaacatg cccatttcca gtccgcctag gttgatatca
      361 tgtgtcgcaa ctgcgtcccc ttcatggaag atttcgattt taaatccagc acacggatgg
      421 atttcaacaa attcactgcc attgattcgt ccaaaacttg ttgtgatttg tttgatccaa
      481 agatcgccga tatgacggcg gttgatcgtc caggtttctc catttctaaa ataaaatttt
      541 agcgctgtca tttctttact cattaaaaag actcctttat gatgtaattt attcttgtac
      601 tacctcagtt tagcacaaat ttatgtaagt ggtcacatct tatgtttttt cttctaatga
      661 cgtatctgcc cactttaagt agccaacaaa gctgagcaga tacagtctag tgttttttaa
      721 atcaattttt ctttcgataa aaaatccaaa acctgttttt tatcattttt tattctttgt
      781 tctacgacgg ctttttcagc taattgcaaa gcttcactaa cccacttacc aggttttttt
      841 ccagtttctt ctagcaagat tttaccatct actgctaatt cgcttaatga cttgataggt
      901 aaacgatcat aggattctcc tacggcatcc atctgaggtg tttcgtcaaa atagatcatc
      961 aattcttcta cttgcagtgc atgttttttg cctaagaagt acaattcgaa tggttccaaa
     1021 gctccagttt ctcgttgtcg tagtccttgg acaagtgctt gaacgtcttt aatcatttgg
     1081 ttagaacact tccacgattt caaaaaggaa cgaatctcat tttcttttag atccagttgg
     1141 ctgatcaata atgcccatgc ttggctctct tctttcaatg ggacggatgg taattcagca
     1201 aaacgtaaaa gtgcttcgcc ttcttctttc aaaccgggac agcaaatgta gcactctgtt
     1261 tcaataaatg catggattcc cgcggcccgt tcatttccca agagcatctt gatgaattcc
     1321 acatttatac gttctacaga tattttacct aatagctcgt ggttttcttt gatggcgtag
     1381 aaggtttctt cctctaattt gaaccctagt tgactgacaa aacgcaaccc gcgcatcatt
     1441 cgtaaggcat cttcatggaa ccgctcatga ggattgccta cagctcgcaa gatctgttct
     1501 tctagatctt gcagaccatc gaacaaatcg atgatttctc catcttcttt caaagcaaac
     1561 gcattgatcg taaaatctct tcttttcaaa tcttctgcca acgaacgaac gaattcgaca
     1621 tgatctggtc tacggtagtc ctgataagtt gattccgtcc gaaatgtggt gatctcatat
     1681 tgttcatcat tttctaatac caacacagtt ccatgttcga tcccaatatc tatcgttttt
     1741 ggaaaaagct gtttgatttc ttctggaaat gcgcttgttg cgatatccac atcatggatt
     1801 ggctggttca gcaagatatc acgaacactt ccacctacaa aataagcttc atatcctgca
     1861 ttctctaatt ttttcagaac tggaattgcc ttttggtatt ccagaggtaa ttgttttaat
     1921 ttcatttttc caacctttcc tgcgtgtttc ttgtcctgag ataaagactc agttgcatgg
     1981 tgactgtttg acaagtccgt ttcagagtct tcaaggccag gaccacttca tcttgtttac
     2041 tcgtccttac gctcaaagcg attatggtct ctttgattga atttttccat gtttttttca
     2101 aaaacttcag ttaagtcgat attcaatgaa ttagccatga tcatcgtaac gaataaaaca
     2161 tcaccaagtt cttctgctac cgagttaact ggttcagaag cctttttgct tttttctcca
     2221 taagtgtggt tgacttctct tgccaattca cctacttctt cagttaatcg agccatctga
     2281 gctaatggag agaaataccc caccttgaat tgctggatat attcatccac ttcctgctgc
     2341 atactgtata aagaacgctt tttatccatc tttttccctc caaaactatc ttaccaaaac
     2401 tttaacgcaa atcctagata agagcaattg agaaaatcga atttccatgc tacatttagg
     2461 aaggaatttc taaatggaaa ttctgtgaaa atacataatt gaaagctgga attcatatgg
     2521 tcaaacgaaa aacagtaaaa gaagttcttt ttatcattat aggaacaagt atttatgcat
     2581 ttggtcttgt ttacttgaac atcgctaatc atttagcaga agggggagtc tcagggatca
     2641 ccttgatctt aagagcttta tttggtattg atccagctta tacgacttta ttaatcaaca
     2701 tcccattgat tttactagga ggaaagatcc tcggtaaacg ttccttggcc tatactgttc
     2761 taggtacact ttctttgtca ttctttttat ggatctggca aagggttcca ctagaaatca
     2821 atttacagca tgatctactg atcgtttctc tacttgcggg tttagttgca ggtgtaggaa
     2881 gcgggatcgt ctaccgcatg ggtggaacaa caggcggcag tgatattatc gctcggattt
     2941 tagaacaaaa ttacggcatc actatggggc gttccctatt ggcatttgat atcatcgtct
     3001 tacttgcttc attaacctat atcgacttga aacgtatgat gtatacgttg attgtttcgt
     3061 atgttttcag tcgtgtgatt gattcgatct tagatggcgg atattctgca aaaggtctac
     3121 ttgtcgtctc caacaaaagc gaagagattg caccactact gatgaccggt ctgcaaagag
     3181 gtgtgacttt cctttctgga gaaggcgcat tctctggtaa ttcaaaaaaa atcatctata
     3241 tggtcgttag ctctagtgaa ttaactgaag ctaagaggat cgtccacgag attgacgaaa
     3301 aagctttcct ttcaatcatc aatgtgcatg aagtggaagg gcaaggcttt acttatttga
     3361 agccacagac aagattactg aaaaaactat aagaaaaaag acaaagatag gctatgagga
     3421 aagattccac atccagctga cctttgtctt ttttagtact atactatttt taactaattg
     3481 ttatgttcac ttacccggtt acttttcttt tgtgtctgca aaaggatctt tcggttaact
     3541 tcatcagata atttcaaaaa agcatcccta tctccatctt ctagtgcttg atcgatttga
     3601 gcataaagca tttttagttt tgcctcttct tctttttctt gaaaatatcg ttcaattaat
     3661 tcagtatctt cttcactgac tgaagaatcc caagaagaaa atggattgtc ttctaagata
     3721 gatagatatt ctcttgtctg ccaagcattt tcgaatatac attccacata taaaggcttt
     3781 ttccaattca aacggatttc atgaaaaatc tggtctgtgt ccgtaaattc ttttccctgc
     3841 aaaaacaatt tcatgggttc cccttcaaca gatagatcac tgatctgcag tcctcgatcc
     3901 gcaaaattcg ccccttcaac aaagtggaca ttgcttaaaa tcgcttcatg attggcaaga
     3961 taattcaaga tccaaaaaac ttctcttcgg ctaaaagata catgattgac catccacgtt
     4021 aaaaaattct tcttttcccg tacatcaatt agcatttttc tcacctctct gttaggtcat
     4081 ctaataatga cagtgcctcc atatctcctg gttcaagtgt taaataataa gtcaataatt
     4141 cttgcgctct tttcagctgc ccttcttctc ttaagaaaaa ggcatactct ttcaagaaat
     4201 ctggttcatg tgatagttca tgataagcct gttcatagtg tacagctgca acggtaaagt
     4261 cttctaattc attataagca tgggctaggt tccattctgc ataaggatta gcagtttctt
     4321 ccatctgatt gatcacttgt atcacatctt catatctttc atcatccaag taaagattac
     4381 ttaaagtaag taatgtttca tcttgtttgt ctcctacttc taaagctttc agtaaaagtt
     4441 cttctgctcg ctgtctatca tgcagacgga aagcattttc cgaggccagg tgataaagtt
     4501 cgacttggta aggattttct ttgattcctt cttctaacac tgtttgtgct tcttcaatca
     4561 tttcttcttc ttgaagagct tgtcctagat atagatataa cgattgataa tgcggattaa
     4621 cttctcgaag ttcttccaga tagttgattg cctgctgatt ttctttcaat tgaagattcg
     4681 taaaagctag ctggaacagt cgatcatctg tcctttcttc tttcagtgct tcttctaaag
     4741 gtacgatagc tgcttcgaat tctccttgca tacttaagga ttgtcctaaa cgttcctgca
     4801 tcgagattcc tgaaatttca tttatcccgc ttgccaacaa tgtttcataa accactgctg
     4861 cctctttgaa acgatcaact gaaaaataca attcagctag cgcaaactgg atcaatggtt
     4921 catcaggaag caacccagct gcttctttca attttgcttc actcacttca gggattccga
     4981 taacttggta caaatcagct atcgccaata agctttgcgg atagtaatca ctttgcttat
     5041 ccactttttc caggtattca aaagctaagt cgatctcatt atcttcgatt gcaatttctg
     5101 ctaacgggat gttcagtccg tcattttctg ggtattggtt gatcagttgt tcaaaaatct
     5161 gttttgcttc gatcaaaaag ccaatagcca gcaattcttc gcctagttca gctaatatat
     5221 ctgctgggtc ttcttttaat gctttttcta aaaagatttg tgcttgtgac aagtcttcat
     5281 ctgataaagc ttgcaacatt ttttcgctat ttgtattcat ttcaattccc tcttatctca
     5341 ctgacgattt gttgaaatac cgtcattgtt ctcaataatt ctttttcgtt tggatgttgg
     5401 tatccgtcta ttatacctat tttttctaaa acaagtattt ttttgctttt ttctgccaaa
     5461 aaaagaactt tttccaaata ctccatcttt gaaatctgtt ccagccagtg gattggatag
     5521 ccaagcaagt ccagccacac aaaaaagttt tttagttgga aatcaaaagg atatgcttgg
     5581 agattccaca cgaaatgaaa catcattcct aaaaattttt tcatactagc agaaagtaga
     5641 tggccatttt cggtcagata aaaagcttgt tcaaacagtt taccataatt tgacaggttt
     5701 tctgcctcta attgataaaa atagatcatt tcttcgatca tcccggcaaa cggccgagtc
     5761 gttaattgct ttttatttgg atagttttta tacagatttt gcaaaaaacg atgatcacaa
     5821 atcattccgc aacagataaa agtcaaaaag tcaatctgct ttccgtccac ctgccgctcg
     5881 ctgattgttt gatcataaac gatgcgttct ggcagattga tggtttgcag cactacacga
     5941 tttggtagct gcaaaatcgt ccgctgcggt gttaatgctc ggcttagaga acggatcgat
     6001 acagggatca tccatagtga tgaatcaagg gttgtatgtt tttgaaaaaa acctgccaga
     6061 tccatgatcc cttcatttcc aaatccgatg ataaacatgg attgattttc gggatagcgt
     6121 ttgacgaaat caagcaggtt ctttaattct gttaaatgat tgcattgagt gttggcacaa
     6181 atataccagt cattgtttgc ttgatttgca aataacgggt tgatcttatc tgcaaacaaa
     6241 tcataatagc gctgattcgt taaaaataag attggctgat caatggaaag ttctcttagc
     6301 tgactgacta gcgtttctcc gtaaaacact ttcgtcaacg catcttctac ttgataatca
     6361 agttccatac gcatccctcc tcttcttatt tttattttac cattagtaag caaattctag
     6421 aaaagataag agcaaagctc acctcataac ttggaaataa ataaaaatta aataaaatac
     6481 atgtgttttc tatgtatgac atacggattc ggaatctctg tctcttattt attccttttc
     6541 tgctaggaaa taaaaagaaa agcacttgta gccctagagc cacaagcgct ttttcaactg
     6601 tcttatttaa cagcgtcttt taatgcttta cctggtttga atgcaggtac tttgctagct
     6661 ggaatttcga tttcttcacc agtttgtggg ttacgacctt tgcgagctgc gcgttcgcga
     6721 acttcaaagt taccaaaacc gattaattga actttttcgc cgttagctaa agcatcttga
     6781 atagttgaaa atacagcatc tactgctgca gttgcatctt ttttagttaa atcagtagct
     6841 gaagcaactt tttcgattaa ttctgctttg tttgccatgg atagattcac ctcctccaca
     6901 agagatatga tagaaatatc atttagttaa aactttctga gtggtccaga agtttaaaaa
     6961 gaaatattga ttaacttcaa tattctgtta tcacgatatc atatgaaccg cttaatagca
     7021 aggtttttac caattttcac atgctttttt tagtcataga aacgtaacaa acgaaactgt
     7081 ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc ctattttctc ctacgaggga
     7141 taattcggat ggacgtccct tcaaaagtaa atgcttttct gatttgattt tctaagaaac
     7201 gttcataaga aaaatgcatc aattcctctt cattgacaaa aacgacaaat gtaggtggtt
     7261 tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt tcctttatct gttggtgttg
     7321 gattgattgc tacagcatcc ataatgatat cattcaacac agccgaagag atacgaagat
     7381 tctggttcat acttacttgt tcgatcattt caggcagacg ttccaaccgt tgtttagtca
     7441 ctgcagaaac aaaaacgatt ggagcgtaat caagataccg gaattcatca cgaatctctt
     7501 gttcgaaatc acgcattgta ttggtttctt tctttacaag atcccattta ttcacgacga
     7561 taataattcc acgaccagct tcatgggcgt aacccgctac tcgcttgtct tgttcccgaa
     7621 tcccttcttc agcattcagt accatcaaaa cgatatcaga acggtcaatg gcacgcattg
     7681 cacgcatcac gctgtatttt tcagtatttt cgtatacttt tccgcgttta cgcataccag
     7741 ctgtatctat cattaagaat ttctgaccac tttcagaaac gaagtgggtg tcgattgcgt
     7801 ctcgggtcgt tccttcaata tccgaaacaa ttacacgttc ttctcctaaa atcgcattga
     7861 tcaaagagga tttaccaaca tttgggcgac caatcaaact aaacttaatc gtgtcgtcgt
     7921 cctcttcttc agtctcttca gagaaatact tcactgcttc atctaaaata tcaccaatcc
     7981 ctagtccatg gcttcctgaa acaggataag gatcgcctag ccctaaggaa taaaattcat
     8041 agatatcgtt tctcatttct ggattatcta ctttattgac tgctaaaatc actggtttat
     8101 tacttctgta taaaattttc gcaaccatct catctgcgtc tgtcacgcct tcgcgtccgc
     8161 tgactacaca aatgattaca tctgcttctt caatcgcgat ttctgcttga tgcttgatct
     8221 gatccataaa aggttcatcg ccaagatcaa ttccgcctgt gtcgatcacg ctgaattctc
     8281 gacctaacca ttcacctttt gcataaatac gatcacgagt tactccagga gtatcttcga
     8341 caatcgagat acgctcacca gcgatgcggt taaaaatcgt agacttaccg acgttcgggc
     8401 ggccaacgat tgcaattgtt ggatttgcca tattatttcc tcctcacaag tttccaatct
     8461 cacttagttt aacgaatgca ttgccaactt tcaagcatag attgtcaatt aaacgaagta
     8521 tttttattcc ttattgaaat actaatcgct tttcaaaaga aaaactgaga caaaagcagc
     8581 tgctttcatc tcagtcacag atacaaaatc aattattttt cagaatcatc ggttgctttt
     8641 tcttctactt cttctttcaa cgcatcacct aaaatgtctc ccatagtaaa tccagtattt
     8701 tcttctggta attcataaga ctcaacattt tttggttctt cttctgaagc tggtttttct
     8761 tctaaagctt tgatactcaa tgcaatacga tgttcttctg gatggatctc taatactttc
     8821 actttcactt cgtcaccttc atgaagcact tcatgtggtg tcgcaatatg tttatgtgaa
     8881 atttgagaga tatggactag cccttctact ccagggaaca cttcaacaaa ggcaccgaag
     8941 cttgtcaaac gtttaactgt tccgtctaat actgtgccaa ctggagcttt tgtatcgatt
     9001 tctgaccaag gtccaggctg tgtgtctttg attgacaagg aaacacgttc ttgttcagga
     9061 ttgatagaca acactttgac atttacatca tcattgacag ccaatacatc acttggtttt
     9121 gctacatgac tatgtgaaat ctctgagaca tggactagac cgtcaattcc gcctaggtcg
     9181 acaaatgcgc caaaatcagt aagacgtgca acacgtccac tgagaacatc tccctcatgg
     9241 atagttgaaa gaatttcttt tttcttttct tctctttcgt tttccactac cgctttatgc
     9301 gataagatca aacgattttc tgatggttcg atttcgatga ttttaaagct caatgttttg
     9361 cctttatagt ctgaaaagtc tgcaacaaaa tgatcttcca ccattgatgc agggacaaaa
     9421 ccacgtacac cgacgtcaac gactaaaccg cctttgacaa catttgtaac aggtgcttcg
     9481 atgaccttgc cctcttggaa atctctttcg atatcttccc aaactttttt agcatctaaa
     9541 cggcgttttg aaagtaggta gctgccgttt tctttatctt tgccgatcgt actgataacg
     9601 actaaatcta atacatcacc gacttttact acttcgctga tatcttctac cggcaaagtt
     9661 gataattctt ttgccggaac gactccttca actccagccc cttcgattcc gacaatcact
     9721 tgtttgtctt caatgactaa aacttcgccc ttaacaacat cgccaacttt tacttcttgt
     9781 acgcttttca ttgcgtcttc cattgactgg ttgttctctt gattttgatc aaattctgtc
     9841 ataacacttg tcctcctaac caacattctg ccgtaagtac ggtactgttt ttagtttaat
     9901 tgattattca aaaaaaataa agcgatttcg ttcgttttcc ttgaaaaaac aattgttttt
     9961 catttttttt cttaatttaa aaaagaagat aacctttttt caaaacaaca gcttttattg
    10021 ctgcaacgac ttctggaatc gatttgccag tcgtatcgat acgaacagca tcctctgctt
    10081 gcttcagcgg agagacgtcc cttgttgaat cataatggtc tctttcctgt atctctttcg
    10141 tcagtgtttc aagatcagtc ggaattcctt tttcttggtt ctctttgaaa cgtctttctg
    10201 cacgctcact tacgcttgct actaaaaaga ttttcacttc tgcattcggt aatacagctg
    10261 tcccgatgtc tcttccgtcc atgacaacgc caccgcgttc ccctattttt cgttggacag
    10321 caactaattc ttctctgact tttccatgtg cagagacttc agaaacagct tttgtgacat
    10381 ctggttgacg gatttctaaa gtcacatctt ttgatgctat gaatacatgc tgtccatctt
    10441 cttgttgcga aaaagagata ggatactttt tgatcagcga aactagccct tcttcatctg
    10501 aaaaagatac attgttcgta atagctaaat aagttactgc gcgatacatg gcaccggtat
    10561 ctgtataaat ataattcaat tcttttgcta atatcttggc aacggtactt ttcccagatg
    10621 aagcaggccc gtcgattgca atactgattt gtgtcatgat ataactcctt tttctcagaa
    10681 aaaaagccgc aaagcgcggc tattattatt taattcttaa ttcttgacca gggtaaagca
    10741 taaagttgtt tggatctaag ccattcaatt caaacaattg atcagtggtg atccctgcac
    10801 gctcagccac ttgttttgga ccttcgcctg cttgaacagt cgttgttgaa ccggctgcag
    10861 ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga atcccctgct tgataagttg
    10921 cctcacttga ggaagtcggt gttgtctctg ctgcaatact gctttctgac gttacacctg
    10981 tatcttgaga cacagtagat tcttccgcga ttgtactaga ttctactgtt gaagtctcag
    11041 ttgactcctc ttttgttgta cttgtagaac tttctactac tgatgtcgaa gtttcagaag
    11101 aagttgctgc tgttgcattg ttgcttgatc catttctgat ccagaaataa gcacctgctg
    11161 gaatagcgat acataagacc agtaaaatca aaaatagcga aagaaataat gtatttcctt
    11221 tcttttgttg tctctgagaa cttcttgatg aaacttcatc atctgtatcg tagattggtt
    11281 gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg atgtctatct tttctgctca
    11341 ctgttttacc tcctaaactc tatcgtgtgt attataccat aggaaaaagt gtcttgtcct
    11401 aaaaaatcaa tgttccatgt tttttttaaa taaatttagt aaaatcgttt cccaatgaag
    11461 ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt tctgcttttg ctccatcaat
    11521 atcacaacaa ttctgcggct tttcggagag tttttcgcca aaataagcta agatgaattt
    11581 tctgcggcag ttttcttcat taatatagcg aagcatctgc tgcaggcgga attctttttc
    11641 tttttcctga cgtttgagcc gttccaccca attttctggt ttttcagact gctggatcag
    11701 ctccatccat tttttttgca gctcatcaaa gggagcttgc tttgtggcat attccaaata
    11761 aagctcaaaa ctttgtcgct gttctctaga aagttgatta aagaaataat ggatacgttc
    11821 atccccactt tgataaaaaa gaattgctgc actttcttcc tgatctctgc ctgctcgacc
    11881 gatttcttgt acatagtttt ctagactgtc tggcaaatca taatggacca cataacggat
    11941 atcagattta tcgatcccca taccaaatgc attcgtagca atgagaaact gcagctgatt
    12001 ttttacaaac tgctgttgaa gttgtcttct ttgagctgca tcaagaccac cgtgatagta
    12061 tccaactgta aaacgttccc tgaataaatg atacaatcgt tcgacttctt tttttgttgc
    12121 acaataaatg attgccgctc cttgtgctct ttccatgaag tgttcaagtt cttgttcttt
    12181 ttgctgcgtt ttccgaacaa aaagcgagat attttgccga ttgactgatt gtctgatctc
    12241 ttttggtggt cgatcaaata gtacatgacg tatatctctt gctactaaat ctgttgctgt
    12301 cgccgttagt gccagtgtga ccgggtttcc taaaagcttt cggatttttc ctaactgttg
    12361 atattctgga cggaaatcaa ctccccattg agacacgcaa tgagcctcat ctactacata
    12421 taacgcaata ttttgttttt tcagatgctc taaaagagaa ggattagtca acatttcggg
    12481 actcaaaaac aaaaatttat attgcgatag atgacggagg acgtatcttc tttcagattg
    12541 cgaaagtaaa ctattgaaag cagctactcg tttctcccct cgtttttgta attgcgtcac
    12601 ttgatcttcc attagagaaa gcagcggtga cacgatgatg accagacctt ccattaaata
    12661 ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta ggcaaaatag cgagagtatc
    12721 ttctccatca agcaacgcct gaataatttc ttcttggccg ggacgaaatg aatcaaatcc
    12781 aaaatatttt tttaattcat ttattaacat tctgtatcgc ttcctttctc aatagataga
    12841 tttgatacaa acgaaattct ccataatcta attgtccatc tacattccat tcgctaaagc
    12901 gccactctcg cacggaatcc ttatttggtt ccaaacgttc gattgtttct gcagagagaa
    12961 tccactcgta tggaaaatca tcaaaaaaca aggcccactc aataagatga tctgttactg
    13021 ttcctctttt taaatgacgg attgccatga tctggtcaat tgtctgacca ttcataaaca
    13081 tctgcttggt gatcatcatg ctttgattga aattttgctg taataatgaa tcgatcaaag
    13141 cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa caaaaggtgg atacatctgc
    13201 tttgttgcag ataaacctct gactcatcat tgttttttgc cagctgataa ggaagcagcc
    13261 ctgttcgctc atttccagat aattggttag ctaaaaaatc tgctgcttct tctggtatct
    13321 ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat taaaatttca cgtggcaatt
    13381 ggctttcact aagccatttt tttagttgga aagtataaaa aggacttgtc tctgctggaa
    13441 gatagtcttg aatacccgtt gccagattag aaattacttg aacagcaaat ttaatgagcc
    13501 gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg caaaccagta tgttctattt
    13561 gatgttcgga caacatatca gcccctgcag gcgttagctt tgcttcattt tcatttattt
    13621 cgatccaacc ttgctgcgca attttttgca aaatttgata aaacttatcc tgcttcaatt
    13681 ctggaaaact gttatggata aacaaaagtt cgtgggtaaa tccataaata agtactgaag
    13741 tcgttcgttt tcccacaagg agttggtaaa gagaactaat ccgcagcttg tgtcctgatg
    13801 aaaatagcga taaaataaaa acaatcatat tattctcctt aagttaggat ggttagaaat
    13861 gtcactacta tgtaaacttg tacctgaacg ctgtattgca tgcggtcttt gtcaaatcga
    13921 agcgccagat attttcgatt atgatgaaga aggaatcgtt ttatttgccc aagaaccaga
    13981 ggctcatcaa cagttcgtga tgccggaaga agaaacatat gtaaaaaaag cgtatcaaaa
    14041 atgtccagtt cgagcgattt tattagaaaa taaaatataa atataaacga aactttaaaa
    14101 acgtacacta agttctctaa acttgaaaat aagccgggat ctgtttctct tgttccgtga
    14161 aatcccgact tattttgaaa gattcatttg ctatctatag ttaattgact agttttttca
    14221 cggaccctat aggacccatt tatattattt caactttttc agccagtttt ccaatgtatt
    14281 gacgatcaca gtttgctggt ctgcgttaga aatttctgcc tctccatctc ctttttgctc
    14341 accatagctg ccaaaacctc catgatttcc gccattgatt gatacatact ctgttctttc
    14401 cggtaaatac tgcttagctt cttgataagc ttcctgattc aatactttat cttttgtcgc
    14461 cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt ccttttttgt cgggataact
    14521 tgccatgaag aatacccctt ttaaatgttt gtctgaatga ttcttattga cataacgact
    14581 tgccattacg ccacctaatg aatggccacc gatcacataa tcggaatttg gaaaatcttc
    14641 tttgacctta tccgctttgt ttccgcccat cactgctaga tcaagtggca ttttgacaat
    14701 agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa atactataac tttctggctc
    14761 tacgagagca ccaggataaa aaataagtaa tggagacttt ccatgattct ttttctctcc
    14821 ttcaaaaaat aagtaatctt ctttgttttt tccttctttt accaacactt gcgcttctga
    14881 cgatggttcg tacgtatttt gctgtatata gtaataaccg gctccaccga gtaatcctaa
    14941 aatgatgact aagccaatta gtattcgtct aaatattttc actttctccc accttttcta
    15001 tctgagtgac ccctttttct taatcaagta tgattatacc ttaaaaaaat aagagagaaa
    15061 tgcaaacttt ccagcatcaa ataaaaaaag acggaatatc catttgaata tccgcccttt
    15121 ttgtatgttt atttttattt agcacataaa cgatacacag cttctgcata aatttccatt
    15181 gctttataca tactacttaa ttcccatcgt tcattcgcct ggtgcataaa gtcaggagta
    15241 tcagggaaca tcgcaccgaa tgccacacac tggttcatcg tacgagcaaa agtagcgccg
    15301 cctgatacaa acggttcagt catgtctcca gtaatatcgc gatatgtccc caatagattt
    15361 ttcactaact cactgtctag tggcacgtat aatgaatcaa ggaagtcaaa ttcttcataa
    15421 gtcaacccat atttttctgc tgttttagtt aatttagcta ctaaatcatc tttcttgaat
    15481 gtaacaggga ttctcatatc tacaccgatt tttgtttgtt ctggtgtgat ttccaaactt
    15541 gcaaagttca tcgttagctc gccggattgt tcatcttctg ttttgcccac gacagcttca
    15601 ccagttgcat tttcttgaac taattttcct aagaaattga ttgggccaaa atcaaatact
    15661 tctgaaagag caattgccag acgagaaata gcattcactc cttgtgctgc atctttcgcg
    15721 tggatacttt taccaagaac gacgatgcct tcaccttgtt cttcgaaatc gaattcgtgt
    15781 tttttcaatg cttcttttac ttcagaaagc ttttctccag agtaaggagc tgcatcagga
    15841 acaacattca acgctcctcc agcttttaca gaaaactcgt tcgttcctgg tcctgtcaag
    15901 taagcttgca ataagccttt ttctgcataa attaaaggaa attcagcatc tggcgcaaat
    15961 ccttgcgtaa ttccttcttc tttttcgtta tatttttcta aacaacgcca taaattttct
    16021 tcatctgtac caaagataaa acgaatacgt gtcttgaatt ccacgccagc atcaatcaaa
    16081 gctttgacgg catacattgc cgcaatagaa ggacctttgt catcttggga tccgcgacca
    16141 actaaccagc cgtcttttat agttggatcg aatggttttg tttcccaatt attttcgtcc
    16201 cctgctggaa caacgtccat atgacacaag ataccgaata attcatctcc agagccaatc
    16261 tctgaataac cgtaatagcc ttctggatct ttaaatgttc taaatcctag cttttcactg
    16321 atttccaata ctttatccaa agcttcaatc acttttttac cgaagggatg tccttgtcct
    16381 gaatctgctt catctagtac ggaaggaatt cggatcaatt cgctcaatga ttctaatgct
    16441 tgttcatgat gttgttctgt cacaaatttt ttcatgatat tatcctccta atatgttaaa
    16501 aagggtcatg tcaaattcct ccgacacaac ctcttcttct caaataatga cgttttaagc
    16561 ctgctccatc agcaagcaga atgttcttaa caaatgatga aagaaaaaac actcactgaa
    16621 tggagctatt gacttgtcca cgtgcttatc gatagccgtt tatcggtttc ttcattctta
    16681 tgatagacct tgtacctgta tttgtctagg tttagaacat tgctgctaca cctaggatca
    16741 cacagatggc tacaaataat atcgcaatta atttaccggt aaatttccac cacactgaaa
    16801 catcgaaacg agcaattgct acagcaccca ttacgatagc agatgttggt gtaattaagt
    16861 ttactaaacc agatgcagat tgataagcag tgattactaa atccttaccg acgccggcaa
    16921 attcacccat aggtcccatg attcccattg ttgcagcagc taagccggaa gttgaaggaa
    16981 tcaagaatga catagggata tagaaaataa atgtaataat gatgaataca cttgaagata
    17041 atgaacttaa tccttgttcg ccccaatgta agatggtgtc tgtgattaat ccattgttca
    17101 taaccacttg gataccacga gcaacagcta ctacgattgc tacaccgatc aaatcagaag
    17161 caccagctaa aaattcagag acaaattcag actctttcat gccataagtg attgctacta
    17221 cgatggacat tactaagaat aacatagtaa tttctgggaa gtaccagtca cctaatggaa
    17281 gcatgctttt acctagaacc gtacctagta ccggaaggtt tgttaaccag tttgtcaatg
    17341 attcaaaaat cgtgaaattc gcattcaagg ttgtccaagg aatcaaactg atgatcatga
    17401 taccaaaagt aataaagaac aatacattaa catgtttttg ttttttcgtg attacttctt
    17461 gacgaccgat tgcttcaata tcgaaatgct tcatatcttc tttacgttgg ttgtatacta
    17521 atgattttgt tggatctttt tcgatctttg atgcataacg atacacatat acaatagaaa
    17581 tagctaacaa aataacgaac atcaagaatc gaagaccaat accttcaccc atcgaaatcc
    17641 caacagcttg tgaagctacg cctgttgcaa aaggatttac cgttgaagct agacagccta
    17701 cttgagaccc tactaatacg attgctacag ctactactgt atcaaagcct acagccatca
    17761 tgactgggat cagtaaagca tagaatggaa tcgtttcttc tgccattccg taagtagagc
    17821 cgcctaatgc aaataacagc atcaatactg gaataagcat tttttctttt cctttgaatt
    17881 tcttcacaac tgaagcaatc ccggcatcta gtgcccctgt tttgttgata atacctaaaa
    17941 atccaccaac aaccaaaatg aagaaagaga ttgggattgc agcaggtgtt tctttagtta
    18001 gctcccccgc tggtacacct agcatcccat tgattggcgc catgaaaata tcccacagcc
    18061 cttgaggatt ttgttgtact cgttcatatg atccagcgac gatgttgcct gcatcgtcta
    18121 cactatagtg cccagctggt ataaaccaag taagtacagc gattactgca ataataataa
    18181 ataagaccgt atacgctgaa ggcatttgaa attttttctt ttttgtttgt tccatcttct
    18241 tcttcctttc ttttccgtta gagacaacaa ttttggctct aatgttattt tatcaaaaag
    18301 agtatcagaa caaaaataat aacacgcatt ttgtaaaact tttgttcaaa aagttcgttt
    18361 ttccctattc cctcctcttt tttcaggttt ttaaagcttt cttctttttc attgtagcgt
    18421 ttcctttttt ttataaacaa acattttcgt cttttaaaag gagaagaatt tcacatttgc
    18481 aaagaattga ttgattttta tgcataaatc aaaaaaatac tcaaaacaaa caaataaaac
    18541 gtttacatta ttgcatgtct tgtatttttt tccaaaaaaa aagaccaaga aatttcttga
    18601 tctttttgat atttatttga ctgctgtatg acgttgttcg aactgatgtt gttttcgact
    18661 taaccatgga agaagttgcg catgcagcac taaaaagaca gcactcacga tgaatccttt
    18721 gatcaagttg aaaggaacga tcccaatcag tacatagttt gctaaagaca tgcccagcat
    18781 ctgtgttgcg ttcagcccta aaaacattaa gtaaagaggc gtaataacga aataatttgc
    18841 gatactcata aaaatagtta gagcgatggt tcctgtaatg acacctaaaa atttgtttct
    18901 tcctttatgc tgtttcttta cactaaagaa ataataaatc gggaatgtaa agatcgttgt
    18961 tgataagaaa ctagcagtgt ctcctaccaa attatccgga gaaaaacctg tagtcagcag
    19021 atgtaatact gaacgcaaga acgctgttgc aactccggcc gctggaccga ataagaacat
    19081 gctgatcaat accgagatat cactaaaatc gatcttcaaa aaactaaaag ccggcatgat
    19141 cggaaaagca aaatactgta aaactaatgc gatcgcagca aacatcgcta ctgcaaccat
    19201 tttctgtaca cgggtgttct tcatttgaaa tcctcctgtt aggactcctc ttcagcgaac
    19261 tttttgacgt ttctctccaa atgaaaaaac tctattttcc agggaaaata gagttagagt
    19321 tatttgatca agcgtcgtca aataagctct atcttcttta tccagactat actgtcggta
    19381 tcggaatttc accgattcag ctattttttt gcaaaatagg tcgtggacta taaccaccgg
    19441 tcgggaattt caccctgccc ctgaagacga accttatata atttttgttt acatattcat
    19501 tatacatatc tattcaaaaa acgcaacatc taacacttat aaaaagtaag tgttcatttt
    19561 gagtggttca gcaaactgct gacttctttt ttgttcagtt cacggtaatc cccaggacgt
    19621 aggccttgta aagtcagatc tccgtatttt tcacgtttta gtttttgcac aggaagacca
    19681 actgcttgaa gcatattttt cacttgatga ttacgtcctt cgtggatcgt caattccacg
    19741 atactgtggt ttgtcttagg atccactgat aaaatttgga atttagctgg agatgttttt
    19801 cgtccttcga tctttattcc ttttgtcaaa ggtgcaagca tcgattttgt agcgatccct
    19861 tttacctttg caacgtaaac tttatccact tcatgttttg gatgtgtcag gcgctgagca
    19921 aaatctccat catttgtcag tagcaagatc cccgaagtat cgtaatccag tcgacctaca
    19981 ggatagatac gttcttttac catcggcaaa agatccgtaa caacttttct tcctttgtca
    20041 tctgcaacag cagaaataac tcctttaggt ttgtacaata aatagtaacc gtattcttct
    20101 tgataaattg ggacttcatc cacttccaca gtgtcatgtt tcccaacttt tatccctaat
    20161 tcccttacaa cttgtccgtt tacttttaca cgtcctgcaa ggatgaattc ttctgctttt
    20221 cgacgagaag tgatgccagc gtgggcaatt actttctgca atctctccat tacaattatt
    20281 ctcctttatt cattccatca aagaatagat ctggtgacac gtcttcttca agtgaaattt
    20341 ccatttcttg tgtatcaggc agttcttcta atgatttcaa accaaaataa tctaagaagt
    20401 agccagttgt tccatataat atcggccgac ctggtccttc tactctacct ttttcttcaa
    20461 tcagtttatg ggcagctaga cgctgtaccg ctcctgaaac ctgaactcct cgaatctgct
    20521 cgatttcagc tcttgtgatt ggctgtttat aagcgatgat tgctaaggtt tcgacggcgg
    20581 cttgagataa tgtgtttgat gtcccttgcg catatcgctt aagtaaggga gccagctctt
    20641 ttttagtcgt taatacataa tggctttccg tttcaaaaat atgcaaagct gagtgcgagt
    20701 tttgagtgta agattctttt aattctttca ctaaacttgt gacttctgct tcgctgagat
    20761 ttaacagtcc gcttaattct tcattactga tcccttcctc gccaacaata aaaagaaggg
    20821 cttcaagttc acttaatttc gtcatgttcg gtatctccta ctgctggata aagcataatt
    20881 gattcatagt tttctttttg ttgcgcaatg atctgccctt ttttcatcaa ttctagcaaa
    20941 gccataaaag tagtgacaac ttcactttta gaaggtgact caaaaaaaga atcgaaagta
    21001 cagccatgcg catttctttt tatacgctct tcgataaata ccattcgttc ttcgatcgtt
    21061 gtgtcatcgg gcgtgatcgt tttttccact ttttgagaaa atttcttacg ttctaagatt
    21121 ttctgcatgg cacggatcaa atcgattttt ttgacttgtc ctcgcttcaa cggcttttca
    21181 gcatccgcta acccatcgat gttcataggt tcttttgtgt aataaagact tcgttcctct
    21241 gctttttctg tcagtctttc agctgcatat ttatatttac ggtattctaa aagctgagtg
    21301 accaattctt ctcgaggatc ttcttcccat aattcttcat cgtctgtgcc gaattcttgg
    21361 acaggaagca atgtcttgct tttgattgcc atcaatgtag cagccatcac taagtattct
    21421 cctgcaacag ctagctcgag tgttttcatt gtatggatat aattcatata ttgctccgtg
    21481 atcgtagcga tcggtatatc atatatatcg atttccattt gctgaatcag atgaagcagc
    21541 agatcgagag gaccctcgaa tacatctaat ttaatattga tctccgtcaa ctcattcact
    21601 ctttcttctt tttctttgct agccattttc caatgtaagg ctctgtgatt tcagttgcac
    21661 ggatggagac atccgggtac ttttcctgta attcatctaa catttgaaag cccatattct
    21721 caccgcgata agacggattc aaagaaatgt catggatgat cagctgatcg ttcgttgccc
    21781 agtaaatacc aataatccct tgaatgttgg ttgaaccttc tggcgtaaag aaaaaaagtt
    21841 cataatcgtc ttcagattcg tacgtatcaa tctccttcaa caaagaagtt ttacctgtca
    21901 gtttcttatg gaaactcaat agtcccatag caattttacg gttttctttc cggtaatgtg
    21961 ttaacatctt ctcaccttcc tatgactaag cccttggaaa atgctgctta taaacatccg
    22021 tcattctttt cttcgtgata tgtgtataaa tctgagtcgt agagatatcc gcgtgaccta
    22081 acaattcctg tactgtccgc aaatcagctc cattttctag cagatgtgtg gcaaagctat
    22141 gccgcagcgt atgtggtgtt acttctttgt aaatcccagc ctctcgtact aactgcttca
    22201 gatttttcca gattccttgc cgagaaagac ctttcccatg attattgaca aatacatgca
    22261 tttcagatgc gtcctttaca agtaatggac gtgcttcgtc taagtagcgt tccagccatt
    22321 ggatcgcata atcacctaaa ggaatgatcc gctctttatc tccttttcca actgtctgta
    22381 ccagtccaag agaaagatga agatctccca atttcaatcc aatcaattca ctgacgcgca
    22441 tcccagtagc atacatgact tccaagattg cacgatctct tatccctaac gtttttgtcg
    22501 tatcaggtgc ttcaatcagc ttttctactt ctgtcagagt caatgtactt ggtaatttct
    22561 gtgctttctt cggcgtatcg atatgttgca tcggatcatg atcagtgata cgctcttgcc
    22621 gtaaaaactg atggaatctt ctgagacttg agatcatccg agtaacagtt gctgtcgctt
    22681 ttttttcttc atgcaacttc tccagaaaac ggatgactaa aaagcgatcg acatcctgcc
    22741 atgaagcaat tttctgctcc tgtagaaaat gcaagtactg ttccaaatca cgttcatagc
    22801 tttgtcgtgt attggcggac aatccccgtt caatattcaa gtagtggaga taatcgttga
    22861 tttgctcttt cataataatc cccctcatgc ctctctatca tatcagatac cgcttcattc
    22921 tgatagtctt tttccataat ctgagcaaag aaaaagccgt ctagacactc agacggactg
    22981 gttt
//
LOCUS       DACECR010000043        22895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.043, whole genome shotgun sequence.
ACCESSION   DACECR010000043 DACECR010000000
VERSION     DACECR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.043"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            41..286
                     /locus_tag="IWO17_001503"
     CDS             41..286
                     /locus_tag="IWO17_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290386.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            283..696
                     /locus_tag="IWO17_001504"
     CDS             283..696
                     /locus_tag="IWO17_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290387.1"
                     /translation="MIPKFRAWVKPDILSNHLNGVIAEALPNFIYGNCLVLREDLEED
                     KQFEEIIDFLDIELMQSTGMKDKNGVDIFEGDIVKTLDADLKVTFSTIKFEEGAFCVD
                     YKDLGTEFDFLYFVDSPLEVIGNIYENSELLEEQR"
     gene            693..1277
                     /locus_tag="IWO17_001505"
     CDS             693..1277
                     /locus_tag="IWO17_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0290388.1"
                     /translation="MNKQELIDKLSSLVGSIEDFKGISTFHAGKYAGLEHALELIQQL
                     NESQKPVIPQLVAGWIEKSTDNLTKAEKIAYLIKSKDGDSYYFCDWFVRDGILTQEQG
                     EELLAWATRQSYETLLSLYNGYEVEKEPLYEVIIGDLYLIKKFNNRNDFCFDISRSLC
                     TWEKSAYQLTEAEIKEIDERYWPFAVPVEEVVER"
     gene            1274..1573
                     /locus_tag="IWO17_001506"
     CDS             1274..1573
                     /locus_tag="IWO17_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290389.1"
                     /translation="MSDYLTAEQYEYARKTGDWESGKVLTHGQALNDNQKIVLEWLEE
                     YYAKSQWPFPFYVLKHAANDMPTNIFGFYDDFTMKEEFEVLAAFAQWGLKQEEAE"
     gene            1570..1767
                     /locus_tag="IWO17_001507"
     CDS             1570..1767
                     /locus_tag="IWO17_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002381641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290390.1"
                     /translation="MNKRQKNKQFKKQLIKNGYPDKKVKLYCSECGNRLSFKNEYHKR
                     HLLCNEFCFMHSVGLGWNDFL"
     gene            1783..2088
                     /locus_tag="IWO17_001508"
     CDS             1783..2088
                     /locus_tag="IWO17_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290391.1"
                     /translation="MNVQVYLKRDLARFPRSWDEYSCPFEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            2112..2408
                     /locus_tag="IWO17_001509"
     CDS             2112..2408
                     /locus_tag="IWO17_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290392.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDIKLINDK"
     gene            2484..2900
                     /locus_tag="IWO17_001510"
     CDS             2484..2900
                     /locus_tag="IWO17_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0290393.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHVNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            3161..3574
                     /locus_tag="IWO17_001511"
     CDS             3161..3574
                     /locus_tag="IWO17_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290394.1"
                     /translation="MDKEIKATVKLDLTELKELLNKASDQVEQIKYQGMDRKRLIDII
                     EQQRLELIQKEAVCKEYKKHLEQVIEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTP
                     VFNSVKSEKLESISTALVEKAAEMNSLANEVSRLI"
     gene            complement(3567..3746)
                     /locus_tag="IWO17_001512"
     CDS             complement(3567..3746)
                     /locus_tag="IWO17_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAQ0290395.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDMTK"
     gene            4229..4441
                     /locus_tag="IWO17_001513"
     CDS             4229..4441
                     /locus_tag="IWO17_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290396.1"
                     /translation="MNENQLRELFKTNEANQTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNEGQAMEFMMRTFSANNQQRE"
     gene            4448..4672
                     /locus_tag="IWO17_001514"
     CDS             4448..4672
                     /locus_tag="IWO17_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290397.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            4699..5232
                     /locus_tag="IWO17_001515"
     CDS             4699..5232
                     /locus_tag="IWO17_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAQ0290398.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            5219..6463
                     /locus_tag="IWO17_001516"
     CDS             5219..6463
                     /locus_tag="IWO17_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAQ0290399.1"
                     /translation="MGLVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDIAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRVKRMF"
     gene            6476..7972
                     /locus_tag="IWO17_001517"
     CDS             6476..7972
                     /locus_tag="IWO17_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0290400.1"
                     /translation="MDKVNEFEYGTDIHYSNDVNTNYVKFSVESNLHYRFSSAEDLLN
                     DSNTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKDRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNAETSADRGVFDSDKETDTEVEEDEST"
     gene            7959..8222
                     /locus_tag="IWO17_001518"
     CDS             7959..8222
                     /locus_tag="IWO17_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290401.1"
                     /translation="MKARKKPVTVEIVQLKMLSARSYRKCKEFVGEAWIDHNNMPNGL
                     PGIETLEGTMEISDGDYIIKGVHGEFYPCKPDIFIETYEIIEE"
     gene            8227..9183
                     /locus_tag="IWO17_001519"
     CDS             8227..9183
                     /locus_tag="IWO17_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ0290402.1"
                     /translation="MKSQDYFIKREKAWQEQQIKDDKKRMNEIKKRLQYAQDAIQKEI
                     DAQWNSFSNGQKITRSEAMKRANEMDVKAFARKAKKYVKEKDFSPTANKELKLYNLTM
                     RVNRLELLKANIGLELIATFNELDKYFSNELTKAGLDELKRQAGILEMAIAKSGYAKL
                     VEQVINSSFRADGFATFSERLWMYQAELKADLDKLLVRSVTMGRNPKQLAPELSKFLT
                     EEGRENTKFNTQRLMVTETTRVQSGIQRESYLDANIEKYGWVTEPNACRLCKSIAADG
                     PYLVSEMEVGKNMMPVHPFDRCSTYPIVDRDAFEKSLKERRL"
     gene            9261..9488
                     /locus_tag="IWO17_001520"
     CDS             9261..9488
                     /locus_tag="IWO17_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290403.1"
                     /translation="MHLYQNDAQFKEDYDAVKQHLIDVLEPIVKEVYETARKVIEPVV
                     QSITELVQSNPEYFKALQNNMNQSVFQSASM"
     gene            9791..10393
                     /locus_tag="IWO17_001521"
     CDS             9791..10393
                     /locus_tag="IWO17_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ0290404.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPTEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELEAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            10408..11295
                     /locus_tag="IWO17_001522"
     CDS             10408..11295
                     /locus_tag="IWO17_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0290405.1"
                     /translation="MAQTWNPDNVTVYETKEGKIPDKYNTLIINDIMENSKVMQLAKY
                     EEMTDKEKKFEYFAEGPGAYWVGEGEKIQTSKPKWLQATMVAKKLGVIIPVSREYLHY
                     KMSDFFTVMQPKIAEAFYKKFDAAALLNTDNPFPQSLEESVVAAANVIKGPLTYDNIL
                     ALEDALGKNEFEPNAFISNRKNRTELRSAAQTVGTNVEFIYDRSANTIDGLPVVDLKA
                     LGKGELYAGDFNYMFYGIPFNISFKISEDAQLSTLKNEDGTPVNLFEQELVALRATMD
                     VGFMIVKDAAFGKIEPAGE"
     gene            11589..11918
                     /locus_tag="IWO17_001523"
     CDS             11589..11918
                     /locus_tag="IWO17_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HAQ0290406.1"
                     /translation="MVIKDDVKKLLSGSTDDKLEVIERRTRERLASLLGVSVTPESLE
                     YIVFDVTNKRFNRIGQEGMTSYAQEGLSMAFPDSDFSEYASEIDDFKRKDDEELYKPR
                     KGSVRFL"
     gene            11915..12229
                     /locus_tag="IWO17_001524"
     CDS             11915..12229
                     /locus_tag="IWO17_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290407.1"
                     /translation="MRFLDEVIFEKDGEGNRYDPELGEWIEEAPITVTTNANVTDLGT
                     DRSVAIFGDIRQGAKVIRTMPLFVVPKYDRIIFNGKTYKDVAQRSLLSTNSIIVQEVI
                     AK"
     gene            12226..12597
                     /locus_tag="IWO17_001525"
     CDS             12226..12597
                     /locus_tag="IWO17_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ0290408.1"
                     /translation="MKVSLGYKGVDELLKHLEEAVTLRDVQMVVKTNGAELTKRMQEK
                     ARFNGHWEGDVFVHPTGFTRRSIRMWLLEGGFVAQVGPQSDYSPYLEYGTRFMSAQPF
                     VGPAFNVQKAIFMKDMQRLFK"
     gene            12594..12983
                     /locus_tag="IWO17_001526"
     CDS             12594..12983
                     /locus_tag="IWO17_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HAQ0290409.1"
                     /translation="MIKTRDQSLFDEMFARSLALGYQTYDYKPLDDVGYPFVELESSQ
                     TVHNPNKTDIKGTVNLTISVWGLQKKRKQVSDMASALFNQALQIQATEGYYWDLNYQA
                     SGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            12994..13506
                     /locus_tag="IWO17_001527"
     CDS             12994..13506
                     /locus_tag="IWO17_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HAQ0290410.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDD
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            13552..13920
                     /locus_tag="IWO17_001528"
     CDS             13552..13920
                     /locus_tag="IWO17_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290411.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFIDGNMEFGMGLSAKVL
                     PELRSYNANTLSRVLEIANRTEEETITLDEMDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQAE"
     gene            13974..14261
                     /locus_tag="IWO17_001529"
     CDS             13974..14261
                     /locus_tag="IWO17_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290412.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            14278..17100
                     /locus_tag="IWO17_001530"
     CDS             14278..17100
                     /locus_tag="IWO17_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0290413.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEEKSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEEQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQRLVVA
                     IGAIAIAIGPVLYALGMLVKAFQTMKVGLGVLGNGISLFKKLGSAIGFLTSPVGLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTIQEAPGKAADWIKNKWTETKEFFSSIWDGIKEAASSAWEGIVNILTP
                     YVIAIKNVFQPMIDFFTNLWSQIGSIAGSAWEIIKTAVMGPILLLIDLITGNFNQLKE
                     DASMLWTTLTTNIQNIITTFVDIVVGYYTALKDTVINIWNVLTSTIKDVWNSFTTWIK
                     ETTNNIVNSIKQGWNNLKQGTIDLFNNMIQGAKDLWNSFKAWFINLVIGTKDNIIQGW
                     ENLKQGTIDTFNNLVNGAQEVWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDS
                     FLEGLQNAWKSVQDFVGGIGDWIREHKGPIRYDRKLLIPAGQAIMNGLNAGLTNGFAS
                     VQSNVGNMANMIADSFTRTPDIDLSANLKNANRNFTTQIEHSVNYGKNKRPAVFNIRL
                     GNQVFEAFVEDISNIQGKEADINLLF"
     gene            17118..17900
                     /locus_tag="IWO17_001531"
     CDS             17118..17900
                     /locus_tag="IWO17_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0290414.1"
                     /translation="MEWHNPMYEFRDTVKNDSQKTWIPTSALNYDGKFIENYIEGYQT
                     LYVEGREMVSLEIESEAVSIGVRISSQRLPERILTIHFKLEEKNPIEFQRSFNKLMRL
                     LYRDKDVEIHFNDELDMYYYGRYQTCDNIPGNVHSVISSFSIICSDPRKYTRIFETNG
                     IVSEYLPYEVAPISISLKANNDGSLRITNGRQNISVTNSMIKKGDFIEMDIAEGKVFV
                     NGVNKTRILDLTSSFKNFMVRTGDLVKCDNGTPLIRYRGVWL"
     gene            17900..20674
                     /locus_tag="IWO17_001532"
     CDS             17900..20674
                     /locus_tag="IWO17_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0290415.1"
                     /translation="MDKDVYFFDDSQKLIKIVGEDKLFSVVQEKEITPSKDELINDKL
                     AVSMEFDDEIKESAYMAVRESESSFSMYKIIGIADPGSLLIFTGINFGPDELDAYIIN
                     DIRPANEFFQKTIQRVIDFTLGEWRVGHLDSTLPAVSMTFYYCSIREALKNLQTLGCE
                     IVFRCNLSGEGITDKWIEVYKQIGEYSNERYEYGDKALTIEKEVDRSNIYTSLIGRGR
                     GEEVGDGYGRRIEFDQVYWSKSKGDPLNKPTGQIYLEIPEMTEKYGIPTKNGKRRKRE
                     KVIIFEDCEDPVELIQLTYQELVNCSRPLVQFKATIFGADSLGNIIRIHRDDRGYHYE
                     TRIFSVKIDRLTGKVETGLGDNLNTSSTRQASNTQTAIQTLDEKKMTFYESTEVSKWQ
                     SDIIRGAKGGSIIMMNPWDTGKGESRQPYQMVWMNGDSIDTSNHFLVANSEGIGFIDG
                     KFNESNFKTAWTIDGNFNANYIQSGRIRADIFETSFNAVGDQLKLVKGALQIVNSNKK
                     IMELTKKGMEFWNTKESIGTIGTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILI
                     SPKVGKGLVLLGNGKAIYFGDLDVQGKLTVNGKEITGNNSGGSDPGTIPPQLTTEAEK
                     RAWKIWTMLKARGYSEYAAAGILGNIQGEVGASMNPDTEQLGGPAYGIVQWDGSAYPL
                     VGSPTWNGREYVQRLMNTAGIQEDYRSIEAQVKLLDWCMFNGQWLGKVNPTTVSGFKS
                     INDAKSAAYAFEMNFERPASAHPERQNYAQSWYNKLHGLTSPEPGGNFICPIQKPVTV
                     TSECGWRTSPINGGQEFHNGIDLVNGNPNTPVFAALDGEVVQAGANYYDWYGNYVVIK
                     HNNGKWTGYAHLSRIDVSVGQKVQKGAQIGLMGTTGPSTGEHLHFQIMKNYWPQPVVD
                     FENPRNYIQF"
     gene            20687..21595
                     /locus_tag="IWO17_001533"
     CDS             20687..21595
                     /locus_tag="IWO17_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ0290416.1"
                     /translation="MSKWNVVLSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            21595..22215
                     /locus_tag="IWO17_001534"
     CDS             21595..22215
                     /locus_tag="IWO17_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290417.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            22219..22668
                     /locus_tag="IWO17_001535"
     CDS             22219..22668
                     /locus_tag="IWO17_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290418.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            22668..>22895
                     /locus_tag="IWO17_001536"
     CDS             22668..>22895
                     /locus_tag="IWO17_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290419.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTG"
BASE COUNT         7993 a         3546 c         5087 g         6269 t
ORIGIN      
        1 tataaagaaa tattagaaga gtatttgagg aggacagcaa atgataaaaa aactcgttca
       61 attcagcatg gatttatatg atatcgaatc aggagcaaca ctatctgtgg aatcggacca
      121 tctaatcata aattttggtg gaaagcgcca gattattttg tgggtagttg atgatgtact
      181 gtttccagaa attgttcatg atttcgaaga atcaaaagcg gttgagtttg aaatagtgaa
      241 aaaagtaatg gaattgattg aaaaatacga ggaggacagc gaatgatacc gaagtttaga
      301 gcgtgggtaa aacctgatat tttaagcaat catcttaatg gggtgattgc tgaggcatta
      361 cctaatttta tttatggcaa ttgtttagtt ttgagggaag atttagaaga agataagcag
      421 tttgaagaaa ttattgattt tcttgatatt gaactcatgc aatccacagg aatgaaagat
      481 aagaatggtg tggatatatt tgaaggggat attgtcaaaa ctctcgatgc tgatttgaaa
      541 gtaacttttt caacgataaa gtttgaagaa ggcgcatttt gtgttgatta caaagatctc
      601 ggcacggaat ttgatttttt gtactttgta gattcacctt tggaagtcat cgggaatata
      661 tacgagaata gcgagttatt ggaggaacag cgatgaataa acaggaattg attgataaac
      721 tatcaagcct tgtaggtagc atagaggact ttaaaggtat aagtactttt catgctggaa
      781 aatatgcggg tttagaacac gcattagaac taatccaaca actaaatgaa tcgcagaaac
      841 cagttattcc acaactcgtg gcaggttgga tcgaaaaaag taccgacaat ttaacaaaag
      901 ctgaaaaaat agcgtattta atcaaatcca aagatggtga ttcatattat ttctgtgatt
      961 ggtttgtgcg agatggcata ttgacgcaag aacaaggaga agaattactc gcttgggcaa
     1021 cgagacaatc atatgaaaca ctattgagcc tatacaacgg ctacgaggtt gagaaagaac
     1081 cgttgtatga agttattatt ggtgacttat atcttatcaa gaaatttaat aacagaaatg
     1141 atttctgttt tgatattagt cgctcgctat gtacttggga aaaatctgct tatcagctaa
     1201 cagaagcgga aatcaaagaa attgatgaaa gatactggcc atttgctgtg ccagtggaag
     1261 aggtggtaga aagatgagcg attacttaac agcagaacaa tatgaatatg ctagaaaaac
     1321 aggagattgg gaatcgggta aagttctcac tcatggacaa gcgctcaacg acaaccagaa
     1381 aatcgtgttg gagtggttag aagaatatta cgcaaaatca caatggccgt ttccttttta
     1441 tgtgttaaag catgcagcaa acgatatgcc gacgaatatt tttggttttt atgatgattt
     1501 tactatgaaa gaagaatttg aagttctagc agcattcgct caatggggat taaaacagga
     1561 ggaagcggaa tgaataaacg ccaaaaaaat aaacaattta aaaagcagtt aatcaaaaac
     1621 ggttatccag ataagaaagt gaaactttat tgttcagaat gtggaaacag attgagtttt
     1681 aagaatgaat accataaaag acatctattg tgtaacgaat tttgctttat gcatagcgta
     1741 ggtcttgggt ggaatgattt tctatgatga ggaggaagcg gaatgaatgt tcaagtttat
     1801 ttaaaaagag atttggccag gtttcctcga agctgggatg aatattcctg cccattcgag
     1861 gcagaagaag agagctatct aatgtctgct aacatgatag aaattacaag agatgatgcg
     1921 gatgagtttg ataaaaaaag atcatggtac tcggtttcga atccaatgta tacggctgta
     1981 atagaagatt atcatatatc cgacagattt attctggttg atctagaaag acctaagaaa
     2041 cgaattaaac agtatagacg ttgcgggttt tcaacaaaga aatggtgaaa attaaaacag
     2101 gaggaagcgg aatgaaacta aaagacggat tttactccag cagccacggc atcggcggtt
     2161 taatgctaga tatgccgaca aagaacccta aaacacgtaa gaaaccaaaa ttcaaagtcg
     2221 gcgacatggt tcgatgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac
     2281 atgtttataa tcactcagcg atcattcgta ttgaaaacac gatggaatgc gacaagtggt
     2341 tagcgaaaag caaagagaat ttagctgtag cgagattggt ggatattaaa ctaatcaatg
     2401 acaaataaaa aagccggatc gctccgactg atctaataaa ttccacaagt ttattatatc
     2461 acataaagga gcggtttgac ttgatgcaat tgttacgaga ggtagatttc aaacagacaa
     2521 gatgtaatgc gagagatgtg ctgaagaact ttcggcgctt ggagcggatg gcaggtcgct
     2581 ctttgataga cattaagtcg cctatcataa ccgatatgcc gaaggcacca aagcacgtca
     2641 ataaggcaga agacgcgatc attcaaatga tggatataga agcagagaga gacgcgattt
     2701 tagcggcttt gatggctctc agtctgatta gtcgtcagat actctactac agcttctgtg
     2761 acgtaaataa gcactctaat tatgaaatag gtcaattgat acgaggatac ggagagaaga
     2821 atgtagagaa gctgaaatcc atcgcattga tcgaatttgc tgaagcatac aaaaaaggcg
     2881 tattagttca gtatcgttga ttttgtaggg tttttgtagg gatagtgtag ggtttttgag
     2941 tgttttaacg tgatattatg gtagtgtcga aagattagtg ataggtctga gacaaaataa
     3001 taataaaagg aacatcgttt tattattgtt tcacaattaa gcttcgatag acagcaacgg
     3061 aaatattaag aataaggatg tgaatttcaa ctccttctga ttgttcttat tatctatcat
     3121 ccgttgctgt ctattaattt atgtattgga gggaaaacga atggataaag aaatcaaagc
     3181 aactgtcaaa ttagatttga ctgaactaaa agaactgctc aacaaggcta gtgaccaagt
     3241 cgaacaaata aagtatcaag gtatggatcg aaaacggttg attgatatta ttgagcaaca
     3301 acgtttagaa ttgatccaaa aagaagctgt atgtaaagag tacaaaaaac atttagaaca
     3361 agtaattgag tatcattcag tagaaaagta cagatcagtg gtgcaaaaaa atagagcaac
     3421 gagtcctgat cctcgtcaag atgtatcgat tagtatcaag acgccagtat ttaattctgt
     3481 taaatcagaa aagctagaaa gtatctcaac tgctttggta gaaaaagcag ctgaaatgaa
     3541 ctccctagca aatgaagtta gtcgtcttat ttagtcatat cgataacttg agagtctttg
     3601 gtgattccgt tttttataga attaatggtt tgttcagcgg aatatttagt caagtatgtt
     3661 tcgctagttg ctactacttc attgttattg gacttaataa cgaagtaata ttggccatta
     3721 gttgcttctc ttataacaaa gtacaaattt ttcaccacct ataatttatt tcagcggacc
     3781 actcgctgat aattaaaatt atacgcttag tatttatttt cacaatataa atttgtcact
     3841 gtggcggaaa ggtaacgcta ataacttaaa tcagtggtaa tgagcacctg agcttagttt
     3901 atgcagctag gcaagagacc gtcgagggta aaggacaaga ggcatcttga aataaatccc
     3961 ctaaagatag acaattctag aggtgagagt ttttatgatg gagagtctgt aaaatatcgg
     4021 aaaatggtgt tgcaatgttc gattcattgc cagtgacttt ggtttacggt gatccataac
     4081 tgcctgtcag taaaaccgaa tatacaggtt ggaaactata gcgcgctgat gtttctgtaa
     4141 tccacattcc ctcgaaggat agtgggtcct gtattacata ttagatcact cattgagtgg
     4201 tctttttatt ttgaaaggag ttttatctat gaatgaaaac caattaagag agttatttaa
     4261 aacgaatgaa gcaaaccaaa ccatggaggc gacattctat gaaactcaaa aaagcttagc
     4321 gttaatcgca aaacaagcta agtatttcta cgatcagctt attctacaag ggtttaatga
     4381 aggacaggct atggaattta tgatgcgaac cttttctgcc aataaccaac agagagagtg
     4441 atacataatg agaaactact ggtatgtatc attatcaaat aaatatccgc aaccaaacgc
     4501 agatgatcca attagagttg tccaatcagt ccaaatcaaa aagaagtact ccatcattga
     4561 aatgaccaga gaagctacac cgaaagagat cgataagtac aatcttcgtt actgtggcca
     4621 tggatatttt agtgagcaga acatacagac aaatattgag agatattgtt aataaaattg
     4681 aaaggtggtg gcttgaatgt ggtaaatttg acaccaaaac aaaaagcttt tgcggatgaa
     4741 tatataaaaa atggtggtaa tgctactcaa gccgccatta aagcaggtta tagcaaacga
     4801 tcagcaagag taataggtaa ggaaaaccta actaaaccta acataataca gtatattaat
     4861 gaacggctaa atcctatcga aaagaagcgc aaattaagtg ctgaggacgc tttaaatgaa
     4921 ttgatagata tttggcaagg agaagtacaa ataagcgtga gcaagcaaat agaccgcttg
     4981 gagaaaaaca aggttattaa acatatgcaa tatgaatata caccagattt agaaagcaaa
     5041 ttgaaagcct tggatttgta tttgaagtat aaatcgctgt tatcacaaac gcaattagaa
     5101 aaagctcaaa cagaaataaa attaatgcaa gcaaaattag aacaattaca gataaactca
     5161 gagcgttcta ccgaagaaaa acttgatgag ttgttagaaa agattagtgg tgaattagat
     5221 gggactagtt gatatttata acccaaagca aatcgacgtg ttaaataaaa ccattaagaa
     5281 tgattggttt attactttat tacatggagc aaaacgttct gggaaaacaa aaataaacaa
     5341 tgatttgttc ttgtttgaat tgcgacgtgt gcgaaagatt gccgatgaag aagacattaa
     5401 ggaaccaatg tatatcctag caggagtttc aagtgcaaca atccaaaaga acatcttaca
     5461 ggaactatac aacatgtaca gcatagaacc taaattcgat aaacatggaa actttaagtt
     5521 atttggcgtt aaggtcgtac aagcttatac aggaaacatt ggcggtgttg gtgcaattcg
     5581 tggtatgaca gcatatggcg cttatatcaa tgaggcatca ctagctagac aagaagtatt
     5641 tgctgaaatc gtttcacgtt gttcaggaac aggagcgaga atcctagctg acacaaaccc
     5701 tgacaacccg gaacactggc tgaagaaaga gtatatcgac aattcaagta aaaacattca
     5761 atcgttccac tttgaattgg atgataatac ttttttatct gaacgctata gaaataatat
     5821 taaagaatca acaccaagcg gcatgtttta tgatcgtgat atcaaaggtt tatgggtttc
     5881 tgcagaaggt gtcgtttatc gtgattttga tgctagcaag cactacatcc agtcaaaaga
     5941 cttaccacct ttgagcaact tttattgtgg tgttgactgg ggatatgaac actggggttc
     6001 aatcgtagtt ataggtgaaa cggatgacgg aacagcttat ttaatcgaag aacatgctac
     6061 tcaatttgaa gagattgatt attgggtaga tatagcaaaa ggcattcaag agcgttacgg
     6121 cttacgagtg cctttttatt gtgactctgc gagaccggag catgtggcta ggtttgtaag
     6181 agaaaagatt aatgctaaaa atgctcataa agcacggtta tctggagttg aggaagtcgc
     6241 caagagattt aaacaagata aattgtttat ctgtcaagat agagtgatga aatttcggga
     6301 tgaaatttat caatacattt gggacaagaa aaaaggtgag ccaataaaag aatatgatga
     6361 tgtgctagac tccgttcgat atgcgatata tactcatgag cttcttaaga aaccaaaagt
     6421 taatgtcaac gaaaagatta aacgtgttaa acgtatgttt taaggagtgt gagaaatgga
     6481 taaggtaaac gaatttgaat acggtactga tatacattat tcgaacgacg tgaacacaaa
     6541 ttatgtaaag tttagcgtag aatcaaatct tcactataga tttagctcag cagaagattt
     6601 acttaacgat tcaaatactt tagcagcaat gataaaacat catcatgaat atcaggtaaa
     6661 gcggcttagt gtattagatg attattacaa agctagaaat acaaatatca tggataaccg
     6721 tagacgtaga gaaaaggaaa aagcggatca ccgatcagca cataactttg gaaaagttct
     6781 ttgtacgttt gatgttgggt acaacacagg caatcccata aaagtgcaaa tcgaggatac
     6841 aaatcaacaa aaagaaatcg aagagtttaa tactaataat gacatagatg ggttaaatgc
     6901 tgaactctgg cttgatatgg ataagtatgg gagagcctat gagattatct atcgagattc
     6961 agatgataca gattatgttg atttggctaa tgtatttgaa acatttgttg tatatgatac
     7021 tacagtaaaa cgagagccta ttttggctgt acggtatcct aagacaagat tcaacaagga
     7081 tgctgataaa cagtacattc aaccaatcgt atacacaaaa gaaaaaagta tcacttatga
     7141 tgagacgaca ctaacagcaa ttgagttaaa aaatccccag gatgaaccgc atgaatataa
     7201 agaggtacct attacagagt attctcctaa tcgttttcgg atgggcttgt atgaagatgt
     7261 actatctttg attgatctat acgatgcagg gcagtctgat accgccaact atatgactga
     7321 tctaaacgat gctcttctag ttattagtgg tgatattgaa gcagcaggac tatccacaga
     7381 ggacgccatc aagcagaaag aagcgaatat gcttttgctt gaatctggaa ctgatgtgaa
     7441 cggtaataaa acaagtgtga ctgcaggata tatttacaaa caatatgatg tgaacggtgt
     7501 agaagcatac aaagacagag tacgcaagga tatccacgaa atctcaatgg ttcctgatct
     7561 tactgatgac aatttttccg gagtgcaatc aggagaagcg atgaaatata aattatttgg
     7621 atttgaacaa atgacggcaa caaagcaaag gctattcaaa aagggtctta tgcggcgtta
     7681 tcgtcttttg tttagcctaa aatcaagtat ttctgaaatg gataactccg atttgaaagg
     7741 cttacgtgta atatttacgc ctaatctacc taaagccatt ctggaagagt tgaaatcttt
     7801 ggttgatgct ggagctgaac tcagtcaaga gacgatctta ggactcgctt cttttgttcc
     7861 agatgtacag gcagaattaa aacgtgtgaa tgctgaaacg tcagcggaca gaggtgtgtt
     7921 tgacagtgac aaagaaactg atacggaggt tgaagaagat gaaagcacgt aaaaaaccag
     7981 ttactgtcga gattgtgcaa cttaaaatgc tatctgcacg ttcatatcgc aaatgcaaag
     8041 aattcgttgg tgaagcatgg attgaccata ataatatgcc caatggatta cctggtattg
     8101 aaacattaga aggcacaatg gaaatatctg atggtgatta catcatcaaa ggcgttcatg
     8161 gtgaatttta tccgtgcaaa ccagatatct ttattgagac ctacgaaatc atcgaggagt
     8221 gattaagtga aatcacaaga ttacttcatc aaaagggaga aagcttggca agagcaacag
     8281 attaaagatg acaagaaacg catgaatgag atcaagaagc gtctgcaata cgcgcaggat
     8341 gcgatacaga aagagataga cgcacaatgg aatagttttt caaacgggca gaaaattact
     8401 cgtagcgaag caatgaagcg tgctaatgaa atggatgtaa aagcatttgc tagaaaggct
     8461 aagaagtatg ttaaagagaa agacttctca cctacagcga acaaagaatt gaagctatac
     8521 aatctaacga tgcgtgtcaa tcgactggaa ttgttaaaag ccaatattgg tttagagctt
     8581 atagctacgt ttaacgagct agataagtat ttctcgaatg aacttacaaa agcagggcta
     8641 gacgagctta agcggcaagc tggcatctta gaaatggcga ttgctaaaag cggttatgca
     8701 aaattagtgg agcaagtgat aaacagctcg tttcgagcgg atggatttgc aacgttcagc
     8761 gaaaggctat ggatgtacca agcagagttg aaagcagatt tggataagtt gcttgttcga
     8821 agtgtgacga tgggacgtaa tcctaaacag ctcgctccag aattaagtaa gtttctaaca
     8881 gaagaaggac gagaaaatac taagtttaat acgcaacgtt taatggtaac tgaaacaaca
     8941 agagttcaaa gcggtataca gagggaaagt tacttagatg cgaatattga gaaatatgga
     9001 tgggtaactg aacctaatgc ttgccgatta tgtaagagca ttgccgctga cggtccatat
     9061 ttagtgagtg aaatggaagt aggtaagaat atgatgccag tacatccttt tgatagatgt
     9121 tctacttatc cgattgttga tcgtgatgct tttgagaagt cacttaaaga aagaaggtta
     9181 taacaatgaa gaaagattca tttcaagtta tatctgacag atttttagaa acggcatatg
     9241 ggaaaagcaa agaggaaatt ttgcatttgt atcaaaatga tgctcaattt aaagaagact
     9301 atgatgccgt taaacagcat ttaattgatg tcttagaacc aatcgtaaaa gaagtgtatg
     9361 aaacggctag aaaggttatt gaacctgtcg tgcaatcaat aaccgaattg gttcagtcta
     9421 acccagagta cttcaaagct ttgcaaaaca atatgaatca aagcgtgttc caaagcgctt
     9481 ctatgtagag gagagtacgc atatggaaaa tgaagaagca attgttagag ctatcgcaag
     9541 tttaaataag attctacaaa acgaaaaagt gctaagagta tccgttgaaa ctgatagtga
     9601 atgggaagac ggtattcaat cgattgatat cagcgtgaaa tatttacctt caggagatgt
     9661 aagtgaagcc gaataacggg ctttttatta tgccctctac tgctcggggc aatataaatt
     9721 gagctgttcc gactgaccgg cgtaactggt caaatttatc gggtagcggc gtaaccgtgg
     9781 aggattaatc atgaaaaaac gtttatttat gccaatgaac ttacaatttt tttctgaacc
     9841 aggagatggt ggatctggtg atgagggaca acaaggaaac ctaccagctg gctcacaaga
     9901 gacaccgacc gaagcaaaag aagaaaacaa tactggcaaa acattttctc gtgatgaagt
     9961 agcgaaaatg atcgctgctg agacgaataa agcaaaagca gcgtgggaaa aagaactaga
    10021 agcaaaaaaa gaagaagcta aaaagctggc aaaaatgaat gcggaagaaa aactacagca
    10081 tgagttggaa caaaaagaag ctgaaatcgc tgaattaaag cgtggacagg cactatctga
    10141 aatgacgaaa gaagcttcta aaatgctgac agatgcaaat ttaccacacg atgatgattt
    10201 acttggtctg attgtttctg atgatgcaga tgccacaaaa caagctgtag cagtcatcac
    10261 taactttgct tctttgatta agagagaaaa cgcaagacaa acaccaccaa atgaaggtgg
    10321 acaatttaca gcatcgaaaa atactaaaga aacagtggct aaactagctg ctaaaaatcg
    10381 aattatcaaa taggaggaat aaacaatatg gcacaaacat ggaatccaga taacgtaaca
    10441 gtttatgaaa ctaaagaggg taaaatccct gacaagtata atactttgat tatcaacgac
    10501 attatggaaa actcgaaagt aatgcaattg gcgaaatacg aagaaatgac agataaggaa
    10561 aagaaatttg aatactttgc tgaaggtcct ggcgcatact gggttggtga aggtgagaag
    10621 attcaaacat ctaaacctaa atggttgcaa gcgacaatgg tagctaaaaa gctaggcgtg
    10681 attattcctg tgtctcgtga atacttgcac tacaaaatgt cagatttctt cactgttatg
    10741 caacctaaaa ttgctgaagc tttctacaaa aagtttgatg cggctgcatt actaaacact
    10801 gacaatccgt ttccacagtc tttagaagaa tctgtcgttg cagcggctaa tgttatcaaa
    10861 ggtcctttga cttatgacaa cattttggca ttagaagatg ctttaggcaa aaatgaattt
    10921 gaaccaaatg cgttcatctc aaatcgtaaa aaccgcacag aattgcgttc tgccgctcaa
    10981 acagtcggta caaacgttga atttatctat gaccgctcgg cgaatacgat tgatggtctt
    11041 cctgtggttg acttgaaagc attgggtaaa ggtgaattgt acgctggtga ctttaactat
    11101 atgttctatg gtatcccatt caacatttcg ttcaagattt cagaagatgc gcaattatct
    11161 acgcttaaaa acgaagatgg cactcctgta aacttgtttg aacaagagct ggttgcgttg
    11221 cgtgcaacaa tggatgtcgg ctttatgatc gtcaaagatg ctgcgttcgg taaaattgaa
    11281 ccggcggggg agtaacaatc cccgttatag gcgttactgt aacgcctaaa acctcaagcg
    11341 cagttgcggg gactgctggc aatagacaat taacagctac cgttgcacca caaaacgcaa
    11401 caaataaaac agtgacgtat tcaattgcgc cagtaacaag cggattgacg gtttcttcta
    11461 gcggtaatat cacttggact gacacagtac cagccggtaa atacacgaca acaataaaaa
    11521 cggaagatgg atcacacaca gatactcacg ttttgacact gactgagccg taggaaggat
    11581 aggtacgaat ggtaattaaa gatgacgtta agaagcttct aagcggttct acagatgata
    11641 agctagaagt tatcgagaga cgaactagag agcgcttagc gtcattgcta ggcgtttctg
    11701 ttacaccaga atcactagaa tacatcgttt ttgacgtaac gaacaaacgt tttaaccgaa
    11761 ttgggcaaga gggcatgact tcatacgctc aagagggttt atcgatggct ttccctgatt
    11821 ccgatttttc agaatacgca tcggaaattg atgatttcaa acggaaagat gacgaagaat
    11881 tgtacaaacc tagaaaaggg agcgtgcggt tcttatgaga tttttagatg aggttatctt
    11941 tgaaaaagat ggagaaggaa atcgttacga tccagaatta ggcgagtgga tagaagaagc
    12001 accgattacg gtaacaacaa acgcaaatgt aacggactta ggaacagata gaagtgtggc
    12061 gatttttggg gatatacgac aaggggcgaa ggttatcaga accatgcctc tttttgttgt
    12121 tccaaaatat gaccgcatta tttttaatgg aaagacatac aaagatgtcg ctcaacgcag
    12181 cctgttatca acgaatagca ttatcgtcca ggaggtgatt gcaaagtgaa agtttcattg
    12241 ggttacaaag gtgtggatga gctgcttaaa cacttggaag aagctgtcac gcttagagat
    12301 gtgcaaatgg tagttaaaac aaatggagcc gagcttacta agcgcatgca agaaaaagcc
    12361 cgctttaatg gacattggga aggggatgtg tttgtgcacc cgacaggatt cactagacga
    12421 tcaattcgaa tgtggttgtt ggaaggcggt tttgttgcac aagttggacc gcagagtgat
    12481 tacagtccgt acttagaata tggaactcga ttcatgagcg ctcaaccttt cgttggtccc
    12541 gcatttaatg tgcagaaagc aatctttatg aaagacatgc agaggttatt caaatgatta
    12601 aaacaagaga tcaatcatta tttgatgaaa tgtttgctag gagcctagct cttggatacc
    12661 aaacttacga ctacaaacca ttagacgatg taggttatcc gtttgttgag ttggaaagct
    12721 cccaaacagt ccacaatccg aataaaaccg acatcaaagg aacagtcaat ttgacaattt
    12781 ccgtttgggg attgcagaaa aaacgcaaac aagtgtctga tatggcttcg gcactattta
    12841 atcaagcgtt gcagatacaa gccacagaag gctattattg ggatttaaat tatcaagcaa
    12901 gtggaattca agtgatggac gacacaacaa ccaatacgcc cctaaaacga gcggttgtca
    12961 cacttgaatt tagaattaga taggaggaag aacatggaag cattaaaagg tattgatgtc
    13021 attttgcttt atcgcttatt gaaaaaagaa actcaggaag ctgcttggaa aatggcattt
    13081 caaacagaac acgaaaatgg tttatcaaga gattcagact ctacagtgac aaaagacgga
    13141 aacgttcaaa gtttaagccc agttgaatat gatttttcgg ctacttcaat agttgccaaa
    13201 ggcgattctc atgtagatga aatgaaacaa gccttattaa atggcgatat cattgaaatt
    13261 tgggaaatca acaaagcaga acagggcaca gatgatgatg caaataagta caaagctact
    13321 tattaccaag catatgtgtc tgaatttact ccatcagctg ctgcagagga taacgttgaa
    13381 ttaagtttat catttgcagt aaatggtgtt ggtcaagatg gttatgcaac cttgacagaa
    13441 gatcaagccg atgttgttca atatgcattc aaagataccg tgaaagcaac ttcgacagga
    13501 gcataagagg gcttagatgc tctctttttt attttaggag gatgaaaaac attgaaatta
    13561 aaaattaaag gtaaagaata ttcgtttaaa tttggcacta aatttgtacg tgaattagac
    13621 aaagtgatgc ctttcatcga tggaaatatg gaattcggaa tgggactctc agcaaaagtc
    13681 ttaccggaat tacgttctta taatgccaac acattgtcac gagtcttaga aatagcaaat
    13741 agaacagaag aagaaactat tacgttggat gaaatggatg attacatcga tgaagttaaa
    13801 gacatcgaaa aattgtttga tgaagtccta aaagaattgg cggagtcgaa cgcgggaaag
    13861 ttagcggtcc gaaacctgaa tcagaaattg aaagaagcgg aaaaacaaca agcggaatag
    13921 attctgcact ggcatacgaa caaattctta tcaattcttt tcgatatttg gaaatgacca
    13981 atatctcaga tatcgaaaga atgacgttat atgaatacaa cattcgtatg actgcagccc
    14041 agttatcttg gcttgacaaa gaaaagttga ttcacgaatt agcgtgggca aatcagcaag
    14101 tccaagcgga gaaaaaagta ggcaaaaaga cagttcctgt atatcgatcc tttgaagaat
    14161 tcttcaatta tcaaaaaatc gaagattcaa tcatgggagt ttccgaactt tcaaaacaag
    14221 ataaaaaatt ccaaagctta ctaactaaag ctaactcttg aggaaaggag gaaaatcatg
    14281 gaacaatttt ctgttgaagc cttattaaaa gccacagata gtggatttgt aaagactttt
    14341 aaagatgcgc aagatgctgt taagactttt gaagagaaat caaatagtat gacaaccgct
    14401 gtaggtaaag tgatgcaagg tactggtgcc gcaatgacaa agtatattac cactcctctt
    14461 ataggagtag gcgtagcagc tgctaaagtt ggtggcgact tcgaagaaca aatgagtcgt
    14521 gtaaaagcta tatcaggagc aacaggcgac acatttgaac agatgaaaca gcaagcgatt
    14581 gatctaggag caaaaactgc ttttagcgca aaagaatcgg ctgccggcat ggaaaatctt
    14641 gcttctgctg gatttagcgc acaagaaatc atgaaagcaa tgccgggtct tttagactta
    14701 gcagctgtat ctggagggga tgtggctcta gcttctgaaa atactgctac tgctttgaga
    14761 ggatttggtt tagaagcaag tgaagcagga catgtcgctg atgtatttgc tcgtgctgct
    14821 gcggatacca atgctgaagt tggagacatg ggagaggcat tgaagtatgt tgctcctgta
    14881 gccaattcaa tgggtatttc tttggaagaa actgcagcag ctattggtat tatgagtgac
    14941 gcaggtatta agggttctca agcaggtaca acgttgcgag gagcattgtc taggttagca
    15001 aggccaacaa aggctatgca agatacaatg gataatttag gtgtttcgtt ttatgatgct
    15061 gacggtaaaa tgaaaccttt aaaaactcaa gtagaattac ttaaaaaagc ttttgaaggc
    15121 ctgacgcctg aacaacaaca aaatgcttta gtaacactat atgggcaaga atcattatca
    15181 gggatgatgg ctttgattga taaaggacct gattcattgg gcaaattaac aaaatctctg
    15241 aaagattctg atggtgcagc tgacgatatg gctcggacca tgcaagataa tatgaattct
    15301 tccatcgagc aaatgtttgg agcttttgag tcagcagcta ttgtaattca aaagattcta
    15361 gcaccatcca tcaaaaaagt agcagatgcc atatccggct tagtagaaaa atttgtaagt
    15421 gctccagaat caactcaaag attagtagtg gccataggag caatcgctat tgcaattggg
    15481 ccagtattgt atgcattagg aatgctggtt aaagcgtttc aaaccatgaa agtggggtta
    15541 ggtgtattag gtaacggaat ctctttgttc aagaaattag gttccgccat aggttttctt
    15601 accagtccag tcggattggt tatagctgcg gtagcactac ttgttgtagg tttcatctat
    15661 ctttggaata cgagtgaaga ttttagaaac ttttggattg gcttatggga gggaatcaag
    15721 tctgctgtaa gctcggcagt agaatggatt cagaatgcat ggaaatctac aggagaatgg
    15781 tttaacaatt tatggaagtc cattaaagaa ggcgcagaca atgtttggac tacaattcaa
    15841 gaagcccctg ggaaagcggc agattggatc aagaataaat ggactgaaac aaaagagttc
    15901 ttttcgagta tatgggatgg catcaaagaa gctgccagtt ccgcttggga aggaattgta
    15961 aacattctaa caccgtatgt tattgccata aaaaatgttt ttcagccaat gattgatttc
    16021 tttacgaacc tatggtctca aattggatca atcgcaggtt ctgcatggga aattataaaa
    16081 actgctgtaa tgggtccaat tctacttttg attgatttga taacaggcaa ttttaatcag
    16141 ttaaaagaag atgcttcgat gctgtggact acattaacta caaatatcca aaacattatc
    16201 acgacgtttg tagatatagt tgttggttat tacacagcct taaaggatac tgtgataaat
    16261 atctggaatg tgttgacttc taccatcaaa gatgtgtgga attcttttac tacatggatc
    16321 aaagagacaa ctaacaatat tgtaaatagt attaaacagg ggtggaataa cctaaaacaa
    16381 gggacaatcg atctgtttaa taatatgatt caaggagcga aagatttatg gaattctttc
    16441 aaagcttggt ttattaatct agttattgga actaaggata acatcattca gggatgggaa
    16501 aacctaaaac aaggtactat agatactttc aacaatttag taaatggtgc tcaagaggta
    16561 tgggataatt tagtaaatgc tgttagtgat acggttgata gagtaactgg ctggtttgat
    16621 aacttgaaaa atatcgattt actagcagcc ggaaaagcta tcatggatag ttttctagaa
    16681 gggttacaaa atgcatggaa atctgtgcaa gattttgttg gaggtattgg tgactggatt
    16741 agggaacaca aaggacctat tcggtacgat agaaaattgt taatcccagc tgggcaagca
    16801 atcatgaatg gactaaacgc aggtttaact aatggctttg ccagtgtcca aagcaatgta
    16861 ggaaacatgg ctaatatgat tgcagatagt tttactcgta cacctgatat tgatctttca
    16921 gcgaatttaa aaaatgcaaa tagaaatttc acaacacaaa tagagcatag tgttaactac
    16981 ggcaaaaata aacgtcctgc agttttcaat attcgcttag gaaatcaagt gtttgaagcg
    17041 tttgttgagg atatttcaaa cattcaaggt aaagaggcgg atattaattt attgttctag
    17101 aaagtgaggg aaatatgatg gagtggcata atccaatgta tgaattcaga gacacggtta
    17161 aaaacgacag tcagaaaaca tggataccga cgtctgcatt gaattatgat gggaagttta
    17221 tcgagaatta tatagaaggg taccagactt tgtatgttga aggtagagaa atggtttctt
    17281 tagagattga gagtgaagcg gtgagcatag gtgttcgtat tagttctcaa agacttccag
    17341 agaggatttt aactatacat tttaaattag aagaaaagaa tcctattgaa tttcaacgaa
    17401 gctttaataa attgatgagg ctgctttaca gggataaaga tgtagaaatt cactttaacg
    17461 atgagttgga tatgtattat tacggaaggt atcagacctg tgataatatt ccaggaaacg
    17521 ttcatagtgt aatatctagt ttctctataa tctgttctga tcccagaaaa tatacacgta
    17581 tatttgaaac aaacggcata gtctcagaat atcttccgta tgaagtagct ccgatttcaa
    17641 ttagtttaaa ggctaataat gatgggagct taagaattac aaatggccgt caaaatatta
    17701 gtgtgactaa ctcaatgata aaaaaaggtg actttatcga gatggacata gctgaaggaa
    17761 aagtttttgt gaatggagtg aataaaacga gaattcttga tcttaccagc tcatttaaaa
    17821 actttatggt tagaactggt gatctagtta agtgtgataa tggcactccc ttaatacgat
    17881 atagaggagt gtggctgtaa tggacaaaga tgtttatttt ttcgacgata gccaaaagct
    17941 tattaaaata gtaggggaag ataaactttt ttcggtagtt caagaaaaag aaatcacacc
    18001 aagtaaagat gagttaatta atgataagtt agcagttagc atggagtttg atgacgaaat
    18061 taaagagtca gcttatatgg cggttcgtga aagcgagtcg tctttttcta tgtataaaat
    18121 tatcggaatt gctgatccgg gttcattgtt gatattcact gggattaatt ttgggcctga
    18181 tgaattggat gcttatatca ttaatgatat tcgtccggct aacgaatttt ttcagaaaac
    18241 catccagaga gtcatagatt ttacattagg tgagtggcga gttggtcatt tagattcaac
    18301 tctgccagcg gtttctatga ctttttacta ctgtagtatc cgtgaagcct taaaaaatct
    18361 acaaacgtta ggatgcgaga ttgtttttag gtgcaatcta agtggagaag gaatcacgga
    18421 taaatggata gaagtttaca agcagattgg cgaatacagt aatgagcgat atgagtacgg
    18481 cgataaagcc ttgacaattg aaaaagaagt agatcgaagt aacatctata cttctctaat
    18541 cggccgcggc cgtggtgaag aggttggaga tggttatggt cgtcgaattg agtttgatca
    18601 agtatactgg tccaaatcaa aaggggatcc gttaaacaag cctactggcc agatatattt
    18661 ggaaatccct gagatgactg aaaaatatgg tattcctact aaaaacggaa aacgtcgtaa
    18721 gcgtgagaag gtaattatat tcgaagactg cgaagatcct gttgaactaa ttcagcttac
    18781 ctatcaagaa ttggttaact gttcacgtcc gctagttcaa tttaaagcaa ctatttttgg
    18841 agcagatagt ttaggtaata ttatacgtat tcatcgtgat gaccgcggct atcattacga
    18901 gactagaatt ttcagtgtga agattgatcg attaacagga aaagtcgaaa ctggcttagg
    18961 tgataattta aatacttcat caacacgtca agcttcaaat actcaaactg ccatacagac
    19021 ccttgatgag aagaagatga ccttttatga gtccacagaa gtttctaaat ggcagtcgga
    19081 tatcattcgc ggagcaaagg gtggatcaat tatcatgatg aatccttggg atactggcaa
    19141 aggcgaaagt cgtcaaccat atcagatggt ttggatgaat ggggatagta ttgatacttc
    19201 taaccatttt cttgtagcta attcggaagg gattggtttt attgatggga aattcaatga
    19261 gtcaaatttc aaaacggcat ggacgattga tggaaacttc aatgccaatt atatccaatc
    19321 tggacgtatt agagcagata tttttgaaac ttcatttaac gctgttggtg atcagctcaa
    19381 gttagtaaaa ggagctttgc aaattgtaaa cagcaataaa aaaatcatgg aattaaccaa
    19441 aaaagggatg gagttctgga ataccaaaga atccattggc acaattggta ctactgattc
    19501 tgctggtaat ccttttcctg gagcttctac tcccacacct attgaagata attctttagt
    19561 tattcgtaca aatggagacg gcaaatatat tttgatttct cctaaagttg gtaaaggatt
    19621 agttttatta ggaaatggta aagcaattta ttttggagac ttagatgtac aaggcaaact
    19681 cacagttaac ggaaaagaaa tcacagggaa caacagtgga ggaagtgatc ctggaactat
    19741 ccctcctcaa ttgacgacgg aagctgaaaa aagagcatgg aaaatttgga caatgctgaa
    19801 agctcgtggc tattctgaat atgcagctgc aggtattcta ggaaatattc agggggaggt
    19861 tggagcaagt atgaaccctg atacagaaca acttggtggt ccagcttatg ggattgttca
    19921 atgggatggt tctgcttatc cgttagtcgg atcaccaact tggaacggac gagagtatgt
    19981 tcagcgtttg atgaacaccg cagggattca agaagattat cgaagtattg aagcccaagt
    20041 aaaattatta gattggtgta tgttcaacgg tcaatggctc ggaaaagtaa atccaaccac
    20101 agtatcagga tttaaatcga tcaatgatgc caaaagtgca gcgtatgctt ttgaaatgaa
    20161 cttcgaacgc ccagcttctg cacatccaga acgccaaaat tatgcccaat cttggtataa
    20221 caaattacat ggattaacta gtccagaacc tggagggaat ttcatttgtc caattcaaaa
    20281 accagtgaca gttacttcag aatgtggatg gagaactagt ccaataaatg gcggccaaga
    20341 atttcataat ggaattgatc ttgtaaatgg aaatcctaat acacctgttt ttgcagcatt
    20401 agatggggaa gttgttcagg ctggtgctaa ttattatgac tggtatggca attacgtggt
    20461 tattaaacat aataatggga agtggacagg ttatgctcat ttgtctcgta ttgatgtttc
    20521 tgttggacaa aaagtccaga aaggtgctca aataggcttg atgggaacaa ctggtccatc
    20581 tacaggagaa catctacatt ttcaaattat gaaaaattat tggccacagc cagttgttga
    20641 ttttgagaat ccaagaaatt atatccaatt ttaaggtggt gattctatga gtaaatggaa
    20701 tgtcgtttta agtacaacag aaccatataa ttatgtgggg atgattcaag ttcgacaagg
    20761 caataagaat acagaggtta tggaagcgac tatagttgaa aatggtcttc cctacgattt
    20821 atcagaatgt aaggtatatt ttgaatcagt tgtaggtggg aaatatccag tccaattaga
    20881 aacaaaaatt gtggatgcta aaaaaggaaa aattaactat atttttgata aatattccat
    20941 gcagtgttta catcgacaaa cagccaattt cattatattt aaaggagaag acttgattgg
    21001 aacaactcaa gacttctctt attttgtcat taatgctgtt tcaaaaacag aaggagaaat
    21061 gggttcttat tggcaatcaa tcgaagattt aattgcggat atgacggact ttattaatga
    21121 aaataaaggc gattttacgg actggatgaa tgaaaggaaa gaagaatttg aacgttggag
    21181 acaagaacag gaacacagtt tccaagattg gagagaagga caagaatcag actatttggc
    21241 atggtttgaa tcaatcaagg atattttaaa gacgattgat ccaggtggag tcatgttagc
    21301 cgaactaacg gatgcgcgag tagatattca aggagttcgt catgcgtcta tttcagagcg
    21361 tttgctggca gatatggact atttatatca gaaattgcga gcaacgcttt tcacgattga
    21421 atacggggag attgaagtga ctgatatttt gcaggatgat ctcttttcag ataatcatga
    21481 agtcgaaaaa gttggaactg tagcattccc gattgaagaa ggagccttga tcatcgcaac
    21541 cgttgatgat ccaaagcaaa atgtgttcac tcttgagaaa gttggggtga tctaatggct
    21601 aaaactaaac gaatgatgga aacggatgaa aaaacgggtg tacaacgcca attctttcca
    21661 atcacacatg cttccgcagt tcttgggtta gaagaaataa tagcaggaga agcaacagtt
    21721 ttatctgtta atggaaaaat tggagccgtc gtcattacta aagaagattt gggattagag
    21781 aatgttctca cagaattacc ctatgcaagt gaagaagatg acggcattat cacggcagaa
    21841 atgtatcaaa aaattttaaa cagtggagaa ggtgactacg tgttgccagt tgccactatc
    21901 gaccgtttgg gtggcataaa agttggtgaa ttattgacga ttgatgagac agggaaagtc
    21961 tctgcagtca gacaatctga tgtcaatttt tcgctggagt taaaagaaaa actcgattca
    22021 ctgaaaaatt atactgctgg agaaaacatc actattgatg aagatggaac aattagttct
    22081 acaggtggta gtgggactgg tggtgtcaat cagtcatatg tagatcaaaa attccaagaa
    22141 gctgtaaatc aagcagaaaa ttacacaaac gaaagaattc caaattttac ttttgaaaaa
    22201 attggggagg tatagagaat gacagatatc gtaaaaataa aacaaagcaa tgtacaggtt
    22261 tatccacaaa ctcattggaa tgctatagaa ggcaaaccaa caacggtgaa gggagataaa
    22321 ggcgatcctg gccaagcagc aacaattacc ataggaaccg tttctagtgg ttcaaccgct
    22381 tctgttacga atgtaggaac atcatctgca gctagattta attttgtttt accaaaggga
    22441 gataaaggtg atcctggaat aaatgccaca actacagctg tagctacaac tacagcaaat
    22501 gggttgatgt cgtccacaga taaaactaag ttggatggaa ttgcagctgg agcacagaaa
    22561 aatccaggta atgctacaac tacgacagca ggtttaatgt cagcaaccga taaagtgaaa
    22621 cttgatggat tagccaatat tacatttgag aaagtgggga cggtttaatg gctgatattg
    22681 ttcaattaaa agaagacgga gttgctaaat acttaaaaac gcatgtaaaa gctattgatg
    22741 gtaaagaagc attggtgcag acagatggag accaagccat tgcaggacat aagaattttt
    22801 caggttctgt aacaatcaat aataaacgtg ttttgacgac tgatgataac agatatgaag
    22861 tggtaaatct ggttgtcact aacggtaata caggg
//
LOCUS       DACECR010000044        21847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.044, whole genome shotgun sequence.
ACCESSION   DACECR010000044 DACECR010000000
VERSION     DACECR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.044"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(41..1537)
                     /gene="lysS"
                     /locus_tag="IWO17_001537"
     CDS             complement(41..1537)
                     /gene="lysS"
                     /locus_tag="IWO17_001537"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ0290420.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1648..2652)
                     /gene="dusB"
                     /locus_tag="IWO17_001538"
     CDS             complement(1648..2652)
                     /gene="dusB"
                     /locus_tag="IWO17_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ0290421.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2653..3540)
                     /gene="hslO"
                     /locus_tag="IWO17_001539"
     CDS             complement(2653..3540)
                     /gene="hslO"
                     /locus_tag="IWO17_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ0290422.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3757..5868)
                     /gene="hflB"
                     /locus_tag="IWO17_001540"
     CDS             complement(3757..5868)
                     /gene="hflB"
                     /locus_tag="IWO17_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ0290423.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5958..6503)
                     /gene="hpt"
                     /locus_tag="IWO17_001541"
     CDS             complement(5958..6503)
                     /gene="hpt"
                     /locus_tag="IWO17_001541"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ0290424.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6503..7948)
                     /gene="tilS"
                     /locus_tag="IWO17_001542"
     CDS             complement(6503..7948)
                     /gene="tilS"
                     /locus_tag="IWO17_001542"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ0290425.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8068..8538)
                     /locus_tag="IWO17_001543"
     CDS             complement(8068..8538)
                     /locus_tag="IWO17_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ0290426.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8584..9009)
                     /locus_tag="IWO17_001544"
     CDS             complement(8584..9009)
                     /locus_tag="IWO17_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ0290427.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9076..9345)
                     /locus_tag="IWO17_001545"
     CDS             complement(9076..9345)
                     /locus_tag="IWO17_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ0290428.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9356..10951)
                     /locus_tag="IWO17_001546"
     CDS             complement(9356..10951)
                     /locus_tag="IWO17_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0290429.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10966..14487)
                     /gene="mfd"
                     /locus_tag="IWO17_001547"
     CDS             complement(10966..14487)
                     /gene="mfd"
                     /locus_tag="IWO17_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ0290430.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14504..15064)
                     /locus_tag="IWO17_001548"
     CDS             complement(14504..15064)
                     /locus_tag="IWO17_001548"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ0290431.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15417..16391
                     /locus_tag="IWO17_001549"
     CDS             15417..16391
                     /locus_tag="IWO17_001549"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0290432.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16561..17961
                     /locus_tag="IWO17_001550"
     CDS             16561..17961
                     /locus_tag="IWO17_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ0290433.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18039..18761)
                     /locus_tag="IWO17_001551"
     CDS             complement(18039..18761)
                     /locus_tag="IWO17_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0290434.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18840..19346)
                     /locus_tag="IWO17_001552"
     CDS             complement(18840..19346)
                     /locus_tag="IWO17_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0290435.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19469..21268
                     /locus_tag="IWO17_001553"
     CDS             19469..21268
                     /locus_tag="IWO17_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ0290436.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21336..>21847)
                     /locus_tag="IWO17_001554"
     CDS             complement(21336..>21847)
                     /locus_tag="IWO17_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ0290437.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         6053 a         4791 c         3969 g         7034 t
ORIGIN      
        1 ttgacaaaga gccggaattc cttagacagc ttttattcga ttatctcatt gttgggaata
       61 gcaaaacatc ccgaattgat tgtgcatcag ttagcaacat tactaatctg tcgatcccga
      121 tgcctagacc accagttggc ggcataccgt attctaaggc ttccaagaag tcttcgtcta
      181 caccgtgagc ttcgtcattt cctagttcac gttctttttc ttgcgcttcg aatctttctc
      241 tttgatcaat tggatcgttc agttctgtaa aggcattagc aaattcgcgc cccactatga
      301 atagttcgaa tcgatctgtg aatcgaggat cttctggatt tttcttcgct aaaggcgaaa
      361 cttcgactgg atgtccgtat acaaacgtcg gttgtgtcaa tgtttcttca acaaatgttt
      421 caaagaattc attgataaca tgtccgacac ccatagcttc ggttacttct acattgtgtt
      481 tttcggctaa agctaatgct tcttccaatg tcatttcttg ccagaagtct acaccagttt
      541 gttctttgat tgcatctacc atatggatgc gtttaaagtc actttctaag tcgacagctt
      601 gtccatcata tgtgattttt gctgttccta gtactttttc agctgcgtta cggataatac
      661 cttctgtcag attcattaca tcattgaaat cagtatatgc tgtgtatgct tctaacatcg
      721 taaattctgg attatgtgta gtatcaattc cttcattacg gaaaacgcgg ccgatttcat
      781 aaactttttc cattccgcca acaattagtc gttttaaatg aagttctagc gcaatacgca
      841 aatataaatc aatgtccagt gcattatgat gcgtgataaa tgggcgtgcg gcagctccac
      901 ctgcctcatt gtgcaatact ggtgtttcta cttcaatata gccattgcca tctaaatagc
      961 gacggatttc gctgataatt tggcttcgtt tcataaatcg gtcaaagctt tcgcgattac
     1021 tgattaaatc caagtagcgt tgtctgtagc gttgttcaat attcgttaag ccatggtatt
     1081 tatctggcag tggacgaagt gcttttgaca aaatcgtgat ttctgaagct ttgatagaaa
     1141 cttcgcctgt gtctgttttc atgatttgtc cagttacacc aaagaaatct ccaaggtctg
     1201 catgtttgaa tacttcatag gcctcatcac ctacttggtc ttttcttaca taaatttgaa
     1261 tttggccttc acgatcttgt agatgcgcaa atccagtttt cccttttcct cggcgagtca
     1321 tcatacgacc agctacacta gcggtcaatc ccatttctgc tagttcttct tttgttcttg
     1381 gatcaaatag ttcatgcaat tcttctgagt tgtgcgtgcg ttcaaatcgt tttccaaatg
     1441 gatcgattcc atcttcacgc aattgttcca ttttttgacg acgtacaagc atttgatcat
     1501 ttaaatcttc tacttgatgt tgttgttcct gtgtcacaaa agttcctcca ttctcatgtt
     1561 aatctctttc ctataatgcc aatgaaacac aaaaaaagca agccgaactt ggaatttcgg
     1621 cttgaaaatt cacgaaaaac ttagaaatca caacgtttgt ttcaatagta ctttttccgc
     1681 tttttctaca aattgatcca gcaggtcatt gatttcttgc tgtttttctg tctgattgat
     1741 tgctactttc actttcgctg ctcgaggaat cccttttaaa taataagccg catgtgttct
     1801 gaattctcta caagctactt tttctccttt gagatctgct aagcgttgta aatgaagttt
     1861 agctgtagca attttttcgg ctggtgttgg ttcaggaatc agttctcctg tttcgagata
     1921 atgctgtgtg cggtgaatca tccatggatt gcccagcgct gcacgaccaa tcatcacacc
     1981 gtctgcaccg acatagtcaa gcatccattt tgcatcttct ggcgttttga catccccgtt
     2041 ccccataaat gggatcgtca aatgtttttt cacttctttc aatacttccc agtccgcttt
     2101 tccttcatac atctgaaccc gtgtacgtcc atgcatagcg atggcacttg ctccagctga
     2161 ttgtgctgct aatgcatttt ctacagcaaa gacgtgctct tcatcccaac caatacgcat
     2221 cttgattgtt actgggatat ttactgctga agaaacagct tggaccattt cgtatacttt
     2281 ctcaggatct aaaagccatt ttgctcctgc ttctgctttg attactttat ttactggaca
     2341 gcccatattg atatctatta tatcggcagc tgtgttttct tcgacaaatt gagcagcttc
     2401 aactagacta tctttatttc cgccaaatat ctgtaaactt aatggatgtt ctgtttcttc
     2461 aatgtggagc atctctaaag tttttttgtt tctgaaatga ataccttggt cactgatcat
     2521 ttcacatacg actaagcccg caccaaattc tttgacagtg actcgaaatg ctgaatttgt
     2581 tattccagcc atcggtgcaa caacgacacg attagggatt tccacattgc caattttcca
     2641 tgtgtgttcc acttaaatcc ctcctaaggc ttctttcttc aatccatcta attcttcttc
     2701 gctatacttg tatttgttat tacagaaaga acaaaccgct tctgcaccgt gatcttcgtc
     2761 tatcattgct tggatttctt ctgcacctaa cgcgataatt gcttctgcaa atttttcttt
     2821 tgaacagtca catttgaatt gaacaggcat tttttccaaa atgttcaatt cttctgtacc
     2881 aagcaatcct ttcaatattt cttctggtgt ctgtccttca tctaagaggg tagagattct
     2941 tggtgtcgtt tttaattgtt cttcgatctt gctgattgtt tcttcatcgg cacctggcat
     3001 gatttggatc atgaatcctc ccgccgtctt gatgctgtcg tcagtattga ccaatacact
     3061 cagtccaaca gctgaaggga tttgttctga gatagcaaga tagtaagtaa aatcttcagc
     3121 gatttctcca gaaacaatcg gcgtttgccc agaaaaaggt tcttttaatc ctaagtcttt
     3181 tgtgacagta aacattcctt gtgttccaac tgctccccgc acatcgattt taccttcact
     3241 atttagagga agactgagat gcggattttt gatgtaacct ttcacatctc cctttccatt
     3301 tgcatctact acaattcctg ttgctggacc atcgccttgg attcgaacag ttagtttgtc
     3361 ttctcctttg agcgtcgcac caagtagcag actaccgacc aatgtccgtc ccaaagcagc
     3421 tgaggaggtg tgccaagtat catgacgtct ttgtgcctcc gctacggtat ttgttgcttg
     3481 aacggcgtat gctcggacga atccgtcgtg agcaagtgcc ttaaccaaat aatctttcat
     3541 ttgttatgta ctcctttttc cgtttttaaa ctcatagtga atcatactag caactactgc
     3601 ttttttcaac tttggaatgc ttcttttaaa aataattcag aatcatctag aaaacaggac
     3661 atcaccctat ttacaactta aataaagaaa tctcccgaaa accggttttt gttccggttt
     3721 tcgggagtca tatcagatca attgacctat atttttttat ttgtattgat cgtctgaatg
     3781 attatcttct gattgttgag aatctgaatc atgttctgct ttattttcct cattcagttc
     3841 atgttttgct tcttcaaaat cacgtttttc ttccgcttgt ttttcagcgt ctttttcttc
     3901 caaagcacgt tttgcttctt caaaagtttt cgcttctttt tcgcttggat aatcatttga
     3961 ctcagtacct tcaggcatca caccgtgttc aaacaatgac ttgattgctt tggcatccaa
     4021 tgtttcgaat tccaaaagtt tttcagcaat cagtttatgc tgtgcacgat gagcttcgat
     4081 gatttctcga gctttatcat gagcttccat caagattttt cgtacttctt gatcaatctc
     4141 gaaagcgact tgttccgagt aagcttttgt ttgaccatag tcgcggccaa caaacacttg
     4201 atggtttcct tcatattgaa caggacctaa acgatcactc attccatatt ctgttaccat
     4261 gcttcgtgct agagctgtcg cttgctcaaa gtcattggaa gcaccagttg attggacatt
     4321 gaagatgatt tcttcagctg tacgtccacc aagtaagcct acgatttgtt caaacatatc
     4381 ttctttcgtc atcaagaact gatcttcttt tggtaaagcg atcatatatc cgccggcgcg
     4441 tccgcgaggg atgatcgtta ctttgtggac gacccgagcg cgactcaata ctaatccgac
     4501 aatcgtatga ccagcttcgt gataagcaac catttcgcgt tcttttttgc tgattacgcg
     4561 atctttttta gctggacctg caatcacgcg atcttccgct tcatcgatat cagaagcatc
     4621 gattttcttt ttatttcttc tagcagctac taacgctgct tcattcaaga cattttctaa
     4681 atctgctccg gcaaaacctg gtgtctgttg agccacaact ttcaagtcaa cgtcatctgc
     4741 catcggttta ttccgtgcat ggactcgcaa gattgcttca cgacctttta catctggacg
     4801 tccaactaaa atctggcggt caaaacggcc tggacgtagt aacgctgggt ctagtacatc
     4861 agaacggttg gttgcagcaa tgacgattac tccttcattt ccgtcgaagc catccatttc
     4921 tacaagcaat tggttcaatg tttgttcgcg ttcatcatgt ccgccgccca taccggcacc
     4981 acgttgacga cctaccgcat caatttcatc aatgaaaatg atagctggtg cgtttttctt
     5041 cgctgtttca aacaaatcac gaacacgact tgctccaaca ccgacaaaca tttctacgaa
     5101 atcagaaccc gagatcgagt aaaacggtac tccagcttct ccggcaacag cttttgctaa
     5161 taaggtttta cctgttcctg gaggtccttc taaaagtaca cctgccggga tacgggcacc
     5221 taattcaaca aatcggcgag gatctttcag aaattcaaca acttcaacta gttcttgttt
     5281 ttcttcttcc gcacccgcta catcagaaaa acgtacgcga ttagcttttt tgtctgcttc
     5341 tttggctttt gatttaccaa agttcatgac acggccgcca ccgccaccgc cgccgccttg
     5401 ttgtcccatc atcatgtaaa agaaaaagat gatgattacg attggcaaga agctaagcaa
     5461 tagggaaacc catataccgc tgcttgattc ttcttttact gttgtagcaa ctttgttttc
     5521 ttttgccagt tctgttactt ggctcaatgt tgtatcattt ggcaaaatga ttgttgtgaa
     5581 atgggtagta gtcgtatctg ttgaacctaa gatagctaac ccaccgctgt tagcgacggt
     5641 ctgctgttct ttgtattcac cagtgatctt atatacacca tttgcaggct gcacttcaaa
     5701 ttctttgatc ttgcctgctt gtagttgttc tgtgaatttt gagtactcga tatctgacga
     5761 tcgctggccg ttattaccaa aaatgaaata aacgaccatt accatcgcta agattacgag
     5821 gacataatac aaggcatttt tcatcatgcc gttgttcttt ttgttcatag ttgccctccc
     5881 taagcgtcaa tttaatttaa cagtttttta tcaatcctga ccattctatt gtaccataat
     5941 ttaattgaga tacagcatta atttgattga taaacactag gtttcaatac accgatgtac
     6001 ggaaggttac gatatgcttc tgcataatcc aagccataac cgaccacaaa ttcatttggt
     6061 acgttgaagc caacataatc tgcttcgata tttactactc ttccttcagg tttgtcaagt
     6121 agtgtaacaa ttttgactga ttttgctttg cggtatttga aaagatccac taaataagca
     6181 agtgttcttc cgctgtcaat gatatcttca acgatcaaca gatcgcgacc ttctacattc
     6241 gtatctaagt ctttgacgat ttttacttcc cctgacgaga ctgttgcatt cccgtagctt
     6301 gaaacatcca tgaaatccaa ttccaaatac gtatcgatag aacgaacgat atctgccatg
     6361 aatggtaccg ctccttttaa gacacctact actaggggat tggtgttttg gtaaatttct
     6421 gttaactctt tgcctagttc tttgcaacga acttgtatct cttcttgtga gatcaaaatt
     6481 ctctcaatat ccttttccaa catcaggttc tccttccaat tgcttctttt tgatatttgt
     6541 aaagaagtat gtagtgtatt ttatcagttt cttcacgaat actcaaatat gaaaaggcaa
     6601 aaggtatcaa gctatatata tttcttttgg catctgtaat gatccaagca tgctgccttt
     6661 gtgaattagg tactttctga tcgataaaat atctagatac tttttttgtg agattctctg
     6721 ttaatttaat gcggtctcct gcttttcttt tttcgacata cagttgcaca tcagcaggaa
     6781 tccaaatgtc atgagaaaat agagaccatg aatctgttgc tttgatgtcc cctgcatttt
     6841 caggtttgac cagaccaatc cattctgttt cattcaaata tattccttgt tctggaatca
     6901 gcaacaagcg ctggtcttct gtctggctag ttgcttgttt ttctttttta gagagtacaa
     6961 caaaatgata ctctcttttc agtatccaat tattgccaat actgagttgc cattggcttt
     7021 ttttctgttg ccactggtct aaaatcgact ccatttgttg ttctttcaaa ataattcctg
     7081 tttttgagaa tgctttgtcg aaaaaggagt aaaaaaaata gtatcgttca gcatctgtca
     7141 tttcttcaat catttctgca gaaaatcgaa attgttcctc ccactgttcg acttcctttt
     7201 cgatcaactg tcccatatat ttttgaatga cttggtctgc ccattggatc tgttgcgaaa
     7261 attgctggaa atgtctcaac gcctgtgtat tttcttgttt caattgagga actaccaaat
     7321 gacgaagccg atttctctgg atatctaaca attggtttgt ctgatcctca aagtaaacaa
     7381 gctgcgattc cgctgcataa gtatataggt cttctttgct aaaactcagc aacggacgaa
     7441 tcagtctgcc cgtagcaaat ggttgtactt ctttgattcc agaaaaagta tttaactgtc
     7501 ccccacgaac gattttcatt aacaccgtct cgatttgatc atctccgtga tgagcagtca
     7561 tcaacgtgtc atattgtcct tcttccatca cgcgagtgaa ttcttgataa cgaaagttac
     7621 gagcttctgt ttctatggcg gatttggctg gatcttccca aatacttaca tataaaggga
     7681 gattcttctc ttcacaatat gagcgaagat aggcagcttc ttctgctgat tcttctctta
     7741 attggtgatt gatgtggacg atacctaaag aaaaaccgat ttggcgttgc acttgtaaaa
     7801 gtaaatggag caaaaccatc gaatcaacac cacctgatac cgctgctaaa attctcgatc
     7861 cttgttgcca gaatccttgt tcattcccgt gccgaataaa tgactgctgt aatagcggtg
     7921 cttttttccc gcctttttca acgttcatcc tttccacacc ctttcttcct aaaaaaaaga
     7981 gagcgggaaa acgtctaaaa cgacaattaa cctgccctct gttgatacta tgacccaaat
     8041 agtcgcacta tcattatttg cggcttttta gctgcgacgt ccgccacggc caccgcgctt
     8101 tccttctgtg tttcgcttca atgaagataa gcggtcatcg ctatctttca aaaatgaact
     8161 catcaatgaa tcgaagtctt ctttttgagc tgaaggttga ctgcgagaaa aagatttttt
     8221 ctgtgctgga cgatttcggt tgtcacgggg atctttttgt tgatattttt taggacggct
     8281 ttcttctcca gattcagcag gttgttcctg cgctttccgg atagatagtc cgatttttcc
     8341 atcatcattt actgaaataa ctttaactgt tacttcatct cctacactta aaacatcatg
     8401 gatgtctttt acatagccat ttgacacttc gctaatatga actagacccg tttttccagc
     8461 acctagatca ataaacgcac caaaatttgt gattcctgat acttttcctt gcagttttgc
     8521 tcctacctcg attgacataa aaaaaatgtt cctcctaatt atttcccttt tttatattcc
     8581 tgcttatttt gcagttgtag aactagttga ttgatctgtt tcatttgtcg agctgtctgt
     8641 atttgattct aatataggat aaactttttc accgtctttt gagtaaaaga atttgcttct
     8701 ggcaagttta gctacatact catcatcttt cagcaatgaa acatcttggt gcagctgatc
     8761 aacgtctgcg ttgacttctt tcatttcagt atttgcttcg actctcaatt cgttcaattg
     8821 atgcatcctt tgcatgtcat ggaaaatctg cacacctatt atcgcgaaaa tgaccaaagc
     8881 accaacaaaa attgcagcca gtctgcgtct tcgaaaaatc agttgacgtt gttgttgctg
     8941 aaattcagca tactttttct ttgcatattc cgtatctagc gcagcgattt tattgctttt
     9001 tttcgtcact tggattcgct ccaatctttt tgattgtctt tttcattata ccaatatcgt
     9061 gaaagcttgt ctaatctatt tctctagtgt ttctgtgcga ttttcagaaa taatttcgta
     9121 cattttttgt gcatcttctt ttttggttga ttcatgtaat tccgtaattt tcacttccaa
     9181 cgttttgttg ccaaactgga ctttcaaaat atcgcctact ttgacatcac tggaagattt
     9241 cgccaattta ccgttgattt gaattcttcc tttatcagct acttcttttg ctactgatcg
     9301 tcttttgatg attcgggaaa tttttaaaaa tttatctaat cgcatatctt attcctcact
     9361 ttttaatttt tttatttaat cgcaggattt tcttaccaaa aggcaacatt agccattctc
     9421 gtactgtaaa cagacgaaga cgaatcactg cataaatgaa cacagaagcc ccgatcccaa
     9481 ctcctatcac gctgacaaaa aaagcagtgg aacgatgagt tacaggacca aatactatat
     9541 tcaatacacc ataatacagc agcagtacta ctgtcataca aagaaggctg aacatcaact
     9601 tccgtccaaa atgccgttct ttccaaaaag catttagctc ttttgtttca attcgaacaa
     9661 aataccaaag cgtcacacct aatcctaaaa gggtagacag gcttgcacct gctgttccta
     9721 ataaaccggt cagaaaagaa gtagtcaatc cttttaccaa tagtccccat cctgcgcctt
     9781 ttaaagaagg gcgaaacgag ttcttgcttt gagcaatact ttgataagct tggatgactg
     9841 cagtgaaagc aatggaaaaa acaaataata caagcgctgc atttcccgca taatctttaa
     9901 acaacgcata attgatataa gggagcagca aggctaatcc caaagacgca gctaatgcta
     9961 aagcggtcgt caatctaaga tagattttgg ctgtctgtaa aaactgacga tagatccggt
    10021 tggtcaaatg acgagtcaac gcaggcagga atgtcgcact taatgcagtg gcaatcacta
    10081 aacctaattg tactaaaggc tgacctcgat catagacccc ttttgctatc ttcgcaccgt
    10141 attctgacag tccgtaaact tccaaagcat tttttacaaa gaaagagtcg atcagctgga
    10201 aaaagataag caatccacta taaatcgaaa ccagtccgcc ttctaccaga aaacgccgga
    10261 tcaagggctt tgacggctgt ttgaacaatg gaaaccgtcg gaagcttaac gctcctccat
    10321 gaatcttttg ttcatagtag tgaaggatcc catatgcgca taaaccgcca aaaacagctc
    10381 cactcattgc tgccgtccct gtttggtaca ccgtccatcc aagctttcta aaagcaaacg
    10441 cactacagac aatcacaaaa acacgaacga actgctcaac tacttgagaa acagcagttg
    10501 gttccatcaa gaatcttcct tggaaattcc ctcgataaaa agatagacca ggcatcagca
    10561 aaaaggtaaa ggaaactact tggatcagcg gtttcaattg tatgtctccc atcatccaag
    10621 caatccagct gctaaatgca aagacaaaca accacaaaaa gacacctgtc caaaatacta
    10681 agggataaag attttgcaaa gcttgctttc gctcgaaagg attctttctt tctgcaacgt
    10741 actttgatat gaactgcgga agaccggata aagctaaagt cattgctatc ccataaattg
    10801 gataaacttg ctgataaaca taaaaacctt cgtcgccgac taagttttgg aggggaattc
    10861 ggtaaagggc acttaatact tttgctatga aagaagcaat cgtcaataca aatgctcctt
    10921 gcatgacgcg ccgcatttct ttatgatcca aatcattgct ccttcctacg ctgacagata
    10981 tttttgctca cgcaacgctt tgacaaactt ttggatttcc tgaagccata ctgcttcttc
    11041 catttggttg gggatcgtca agcgtatcac catctgttct ttttcaacgc ctagactggc
    11101 ttttagtttt gtcgctgtca gtgcttcgaa taactgttcc acactatagc gttttgttcc
    11161 aactttactt agagtaaaga ctatatgcgc ctcctgtttc cttatcgttt ctaggagcgc
    11221 tcgatcgcca tccattttga tctgcccaat tgtcaagaga tgtgctactt cttctggata
    11281 ttctccaaaa cggtcgagca gatcatcttc tagttcatct agcatttctt gcgaatcaag
    11341 ttcacgaatt cgtttataga tctcgatttt ctgccgttga tctgccacgt atgtttcagg
    11401 tagatacgca tcgacaccga ggtcgatctc aacagtggtc ttctccactt gactattctt
    11461 tccttgttta cgagaaacag cttcgcttag catttgagaa tacatatcga aaccgactgc
    11521 atcaataaat ccatgctgct gagcacctaa gagattacct gcaccgcgaa tggaaagatc
    11581 ccgcatcgca atcttgaatc cagatcccag ttctgtaaaa tctttgattg cctgaagacg
    11641 tttttcactg acttcgttaa ggatcttctg ctgctcatac ataaagtaag catatgcgac
    11701 acggctgctt cttcctaccc gccctcgcaa ttggtataac gtggataatc ccatataatc
    11761 agcgttctca acgaacaacg tatttgcgtt cgggatatct actcctgttt caatgatggt
    11821 tgtagtgaca agaacatcat attgaccttc aataaattca aaaagcgtat tttctaattg
    11881 cgcctctgtc atttgcccat gagcataacc gatccgtgct tccggaacga gttcttggat
    11941 ttcttctact ttttgttcga tcgtttctac ccgattatat aaatagaata cttgtccacc
    12001 tcgtcccatt tctcgatgga ttgcttcgcg aatagcccca ggattttttt ccataacata
    12061 cgtttggatc ggataacgat tcgcaggcgg tgtttcgatt actgaaagat cccgcacgcc
    12121 taacatcgac atatgcaaag ttctcggaat cggtgttgca gtcagagtca aaacatccac
    12181 ttgtgcacgt aactgtttca atcgttcctt atgttttaca ccaaaacgtt gttcttcgtc
    12241 aatgatcagt agacccaaat cgctaaattc aatatctttt gacaaaattc ggtgggttcc
    12301 tacaacaata tcaacttgac ctgtacgcag ttgttcgatg gtttcttttt gttgctttct
    12361 tgttctgaaa cgacttaata gcccgatatt tacggggaaa ccttcaaatc tttccagcat
    12421 cgtttcatag tgctgctgtg ccaagatcgt tgttggaact aaaaaggcta cttgtttgct
    12481 ttcctttatc gctttaaagg ccgctcgaag cgctacttct gttttcccat aaccaacatc
    12541 acctacgagt agtcgatcca ttggtttttc tttttccata tcccgtttga tctccgctgc
    12601 acttctcaac tggtcatcag tttcagagta ggggaaggca ttttcgaatt ctttttggta
    12661 ggcgtcatca ggtccaaaag catatccttt ttctgactct cttgcggcat ataatttgat
    12721 tagatcgtca gcgatatcct cgatttttga agagactttt cgcttggttt tcgtccattc
    12781 acttccgcct agcttattga tgcgaggcgc tttggcttca gaggcgacat atttttggat
    12841 cagattcagt tgagtaacag gaataaacag tttgtcgtcg ttttgataca aaatagtcat
    12901 atagtcttgg tgaacgccat ctacttctag cgtttccatc ccaatgtatt tcccgatacc
    12961 atgattggca tggacaacgt aatctcctgc ttttaactca ttatagcttt tcaatcgctc
    13021 tgcgtttgtt actgtttgac gtctcgtttg ttttttcgtc gttttttgga aaatttcttt
    13081 ttctgtgata gctactattt tttcttgagg aagttcaaaa ccagcctgca aagctccttc
    13141 aactaattgt acctgtcctc tgatcaatgc atttttttct gtttctacta ctaatatgtc
    13201 ttcatctcga agcatttgtt ctgctttgcg tattctgtcc tttgtcggaa taaagatcac
    13261 aacagtctgt ttttgcttgc gccaacggtc catttctgtt ttcagcaatg gcatttgtcc
    13321 gaagaactgt tgcatcgtcc gatattgaaa attatggatt gcttggaaac gaagatttcc
    13381 catccctttt tggaagagtg agaaaaaggt tgtggtgaac ttttccttct gcacgatttt
    13441 ttgtatatct gctgaaaatg tctgctcaga aaacacccgc aattcgctga ttttttgagt
    13501 ctgccattct gccgcttccc tttcgatttc ccgatttgtt tccatgattc gctgataatc
    13561 atcaacgact agtaaactat ttgaagggaa ataatccaag atcgtcacag attcttggta
    13621 aagaagatct gagtagaatt ttgcttcttc tgtcgggaca cctgcttgcc agcttgaagt
    13681 cagctgaccg aagtaatctt gcaaaaactc gcggtctgct tcttctgtgg tcgcagcaag
    13741 ccgtttttct agcatttttt gcatttgctc tattccatgt tctagatcct ctggtccgta
    13801 gacttgttca ctcgttgggg aaacccagat ttcttccgtt ttttcgattg agcgttgtgt
    13861 ttctgcttca aaataccgca tagaatctat ctctacatca aatagttcta cacgaacagg
    13921 ataggttgtc gttaacgggt aaatatcgat aatgcttcct cgaatactga attctcccgg
    13981 ttttgcgacc atctcttctc gtacatagcc catcaaagtc aattggcgag gaagattttc
    14041 cacgtctatc tcatcaccaa tatgccaatt caattgcgcc tgtagccaag ttttcttatt
    14101 cggcagtcgt ttgtgcaaag atgcgactgg caacaaatag atacctgctt ttttttcttg
    14161 aagtgcgtta agcgttgcaa ctctctcgcc tcgcgcttct ggagaagaaa aggccatttc
    14221 agcagaaagc acttcatcca ctggaaataa atggatctga tcttgcggaa tcacatgttg
    14281 cagatcttct gctaactgat tcgcataata caaatttggc atcagaataa tcatgttttt
    14341 atttgttttc ttaaacgctc gagcaaatag aagtgttttc gctgagcccg ctaatcctgt
    14401 gattagttgt ctaggcattt tttcttctaa attgctgacc cattctttta cttgcggatc
    14461 ttttccgact aattgaataa tatccattcc ttttcctcct tcctcaattg aatcgattca
    14521 tcgcatccac gaacgaatgc ccttctatga gatattcgac tgctttaaca gattgacaca
    14581 tgctttcttc tatcaaaggc tgattttctt tgctgaaagg actaagcaca tgctggacta
    14641 ctgttttttt cccttctggc ctgccgattc ccaccttgat tcgatcaaag acattcgtat
    14701 tcaaatggga aataatactt ttgataccgt tatgcccgcc ggcacttccc ttttgacgga
    14761 gccggatttt tcccactgcc agatccaaat catcgtagat tacgactaat tcctctggat
    14821 aaatcccaaa ataagtcatt agtgggccga ctgcacgacc agattcattc atgaatgttt
    14881 gtggtttgac taaaaggatc ttttctccat tatgaaaaaa ttcggcaact tctgcttcga
    14941 acggattttt tttgaaagta gcttgatgct cgcgtgcaat tttatccacg accataaagc
    15001 cgatattgtg ttttgtatat tggtatttgg tacctgggtt tcctaacccg acgatcattt
    15061 tcatttttgt tttccaccta attctaaaaa gcagcacaaa aggctggact gctaattagc
    15121 ggtccagtcc atcgagcttt tttctttttt atacttgtca atgcatagta taacacaaaa
    15181 caaggcgttt cggctaaaag agaatcgtta ataaatgata aagaaattaa aaatttcttc
    15241 aaagtttaat gaaataattg atatataaaa atagtgaaat aacgcacatg taagctttta
    15301 catataatac aaccttgtta tctgttatat tttatagcca aatagcgtga catggggctt
    15361 gagaaatgtt agcataagca tgggaaaaaa caaatcaatg gaaggattga tactaaatga
    15421 ctgcagctgc agaaaaaaaa gatcatcaaa aagttattct tgttggagac ggcgccgtag
    15481 gttctagcta tgcctttgct ctagttactc aaaatatcgc tcaagaagta ggtattgtgg
    15541 atatcaatac tgctaaaact gaaggagacg caattgactt gtctcacgct ttggcattta
    15601 cttcacctaa aaaaatctat tctgctactt acgcagacgc acatgatgct gacttagttg
    15661 ttattacagc tggcgcacca caaaaaccag gtgaaactcg tttagactta gtaaacaaaa
    15721 acctaaaaat caaccgcgat gttgtcacac aaatcgttga atcaggattt aacggtatct
    15781 ttttagttgc cgctaaccca gtagatattt tgacttactc aacttggaaa ttctcaggtt
    15841 tccctaaaga acgcgtaatc ggttcaggta cctctcttga ctcagctcgt ttccgtcaag
    15901 cacttgcgga attagttgac gttgatgcac gtaacgtcca tgcttacatc ttaggtgaac
    15961 acggagattc agaattccca gtttggtcac atgcaaatgt tgccggcttg caaatctatg
    16021 aatgggtaaa aaacaaccca gaaatcgatg aagaagctat ggttaaccta ttctttggcg
    16081 tacgtgacgc tgcttacaca attatcgaga aaaaaggcgc tactttctat ggtatcgcag
    16141 tagcattagc acgtatcact cgtgccatct tagatgatga aaatgctgtt cttcctttat
    16201 cagtattcat gaatggcgaa tatggattga acgacatcta cattggtgca cctgctgtta
    16261 tcaaccgtca aggtatccaa aaagtaatcg aaattccttt aagtgacagc gagcaagacc
    16321 gtatggctgc ttctgctaag caattaaaag aaattctaga tgaagctttt gctaaactag
    16381 acgctgaata atctttgaac atgacggaaa acatctattc ggtgttttcc gtttttttat
    16441 tttatcaccc ttatggtttc taaatttttt cataaagtag agataacctt cgtcttttcc
    16501 tctaaattat gctatcatga gaccgttcca ccctaaactt ttacttaagg agttgtcgat
    16561 atgacaagat cttcgcatcg aaaaaaatcg aaacatgttg ctttgctttc agttcttggt
    16621 gttctcttag tgctgatcag cttttatacc tatcgcagta catactacac aaagcatttt
    16681 ttgccgaata cagaaataaa tggaataaat gtcagcaatc taacagtcga aaaagcaaat
    16741 gaaaaattaa aagaagctta ttctgataaa aaactggcta ttaaagaaga tggaaaagtc
    16801 tggcaggaag taccaaaatc cgagctcggc tacaaagatg atttcacttc ggagctttct
    16861 ccgatcctaa atgaacaaaa cggatggact tggggaatga cttatgtctc agcggcagaa
    16921 aaacaagaaa tagatcctgt cagtcaaaac caccaaaaac tagacactac cgttcagaca
    16981 ttgacaacta agctgactga tttgaacaaa gaccggacac ctactactga tgcaacgatc
    17041 gaaaaagcag gtgactcttt cgttatcaag cctgaagtca atggaaatac gatcgacgtc
    17101 gatgctgcgg tcaaacaatt aaaaagtgcg gtaaactctg gtaaagatac aattgaattg
    17161 actgaattca aagaaaaacc aaaagtaaca tctgaagaca gctcactgaa agaacaactc
    17221 gcatccatga atgctatcgc aaatgtaaaa gctacttaca gtataaacgg tgagactttc
    17281 cagatcccat caagtgatat tatgagttgg ctgacctaca atgatggaaa agtcgattta
    17341 gatacagaac aagtacgtca atatgtaaca gatttaggca caaagtataa cacaagtacg
    17401 aatgacacaa aatttaaaag tacaaaaaga ggagaagtta cggtcccagt tggcacttac
    17461 agttggacga tccaaacaga cagtgagaca gaagcattga aaaaagcgat tctggcaggc
    17521 caagatttta ctcgttcacc cattgtgcaa ggtggcacaa ccgctgatca tccattgatc
    17581 gaagatacgt atatcgaagt tgatctggag aaccagcata tgtggtatta caaagacgga
    17641 aaagtggccc ttgaaacaga tatcgtttcc ggaaagccaa ctacacctac acctgctgga
    17701 gtcttctatg tttggaataa agaagaagat gcaactttaa aaggaacaaa tgacgatgga
    17761 acaccttacg agagtccggt aaactactgg atgccgattg actggacagg tgttggtatc
    17821 cacgattcag actggcaacc tgaatacggt ggagacttat ggaaaacccg cggttcacac
    17881 ggctgcatca acaccccacc aagtgtaatg aaggaattat tcgggatggt cgaaaaagga
    17941 acgcctgtat tggtcttcta acaaaaataa tatataaaaa gaaagcaccc gtacgattgg
    18001 tacaggtgct ttctttttat tatctaacaa tgtgctactt acatttcagc agcagtaaag
    18061 acttctgaaa catcatcatc gtcttctagc ttatctacta gacgttccaa ttgctctttt
    18121 tgttcctcat ttaaatcaat cgtcgtttgt ggaatcatcg tcaattctgc ttgcgccaaa
    18181 gtgaactctt tttccaactc atctcgtaca gcagcaaaat cttctggtgc agtataaatc
    18241 tcgaatactt caggagaagt ttgcagatct tctgcccccg cctctaatac atcctcaaac
    18301 atcgtctctt catcaacagt caacccatcg cgttcaatag cgatatatcc tttgcggtca
    18361 aacaagtaac tgactgaacc cgtttctccc agtgatccac cgtttcgagt aaaagctact
    18421 cggacatttg tcgctgtccg attacggtta tctgtcaatg catggactaa gattcctact
    18481 ccacccggac catatccttc atacgtgatt tcatcgtaat tcgtttcatc tacggcactc
    18541 gtagcctttt taatcgcccg ttccacattg tcgttaggca tatttgcagc ttttgctttg
    18601 tccacaacaa gccgcaaagc tggattcata gatggatcag gaccgcctgc tttagctgcc
    18661 atataaattt cacgagaaag tttctgaaag actttccctc gttttgcatc ttgggcattt
    18721 tttcttcctt ggatattatg ccatttacta tgtcctgaca tgatttttcc tcccttgttc
    18781 ttttgagaat acacttctat tctagtacta tttcacaaaa aaacaaagcc acagccaatc
    18841 tataaacgct tcttgaatgc cgcaaaaagc acacataata tcacagcagt cagagcaccg
    18901 atcgaatcca acatgacatc ttggaacata ggtgttcgtc caccggtcat catctgatgg
    18961 aattcgtcca aacctgcgta ccctgttgct gaaagccaag caaggatcgc tgtcagccac
    19021 caaattttta gcttggggaa aacacctaaa aacaagctgc ctcccaaaat aaaataggta
    19081 ccgaaatgtg cgcccttcct taaaaagaac tctaaaaatt tggcgtagcc cagatggttg
    19141 atactcacca tacttccacc ataattaaaa cgtatacccg ctaatgtgtc tttaaatggc
    19201 tgattaggca gccagctttc taatcgcgaa acttgtgatt gctgtccgta agtttgagaa
    19261 gaactgtaaa ataagattat aacgactatc agtgcgatta ctaaataaat atttccgttt
    19321 ttcatttgtt tttttgaaga attcataagt cacctctgta ttttatctta gacttttttt
    19381 gaagaaacaa caatcatttt catagagact tcttccataa aaaagtgcta tacttaattt
    19441 tgtattacaa tatgaaggga gttttttcat gagttattca ttaaattgga atctagattc
    19501 tattttttcc ggaggcagtc attctgatgc tttgaaccaa cggatgaagc aattggaaga
    19561 ccaaatgaac gagtattatc accgcgtaac aaaatggtca cctagttctg acaatgcaga
    19621 gcagttaaac gctattcttc aattgcaaga aacgattacg aatggtttta cccaatgcag
    19681 cagctatatt actgcattgc tttctgccaa tgtcaatgat tcagatgcaa aggtcttatc
    19741 aggcaagctt tacgcaatgc tgcctcgtct gcaatcagcc gaaactgtat tgtcaaagaa
    19801 atttgcagaa atatctgaca atgattggaa tcagatgctt tctcaatctt cgtttgaaac
    19861 aattgctttt cgtttaaacg agattcgtcg tgatggctca caattattat cagaagctga
    19921 agaaaacatt ataaatacgc tttctttaga tggtctaaat gcttggagca gtcattatga
    19981 tacgattgta gccagcattt cgattccctt cgaacaagat ggacaagtag tcgaattatc
    20041 tgctggacaa gccttcaata agatgatggg cgatccagat ccaaaagtca gagaaacact
    20101 tttcgcagca tgggaagaag cctggaaaga aaaggcaccc ctcttcacgg atactttgaa
    20161 tcatttagat ggttttagac tttctgacta tgaacttcat ggtgtcacag attttctaca
    20221 aaaaccatta gagtacaatc ggttgaagaa agaaacattg tctgtcatgt gggatacaat
    20281 ccagaaaaac aaacaacccc ttgttaatta cttgactaga aaagctaatc ttttcggtaa
    20341 agaaaaaatg gaatggcaag atcaagatgc acctattatt ttaggcgatt tgaaagaaaa
    20401 aacctttagt tttgacgaag cagcagcatt tattatcgaa aactttaaca aattcagtcc
    20461 aaaaatggct aaatttgccc aatctgcttt tgaaaagagc tggatcgaag cagaagatcg
    20521 tcctgggaaa cgtcctggtg gttattgtac tgagttacca gaaacaaaag aatcaaggat
    20581 ctttatgacc tacagtaatt ctgttaatga ggttgcgaca cttgctcatg aactaggaca
    20641 tgctttccat agcagcacga tgtgggattt accttcttta aatcgcgaat atgccatgaa
    20701 tgtcgcagaa acagcaagta cattcgcaga actgatcgta gccgatgcta cattaaaagt
    20761 cgcaaaaaca aaagaagaaa aaatcaattt gctagataca aaactgcaaa atgctttagc
    20821 tatgttcatg aatatccatt cccgattcat ttttgaaaat cgattctatg aagcacggca
    20881 agaaggatta gtctcagaag ataagatcac agagatgatg gttgaggcgc aaaaagaagg
    20941 ctatcaagga gctttagcta cttaccatcc atatttctgg gctgcaaaat tgcatttctt
    21001 tattgatgac gtaccgtttt ataacttccc atacacattt ggctacctct ttagtttggg
    21061 catctatgct tacgccaacc aaaagggagc aaattttgaa gaagaatata tccatttact
    21121 acgcgatact gcctcaatga cgacggaaga tcttgcgaag aaacatttga atgtcgatct
    21181 tacccaacca gatttttggc aagcaggtat cgacatggtg actgaagata tccaaacgtt
    21241 catggaattg acagaagaat ttatctgatt tctattttat cacaataaaa aaagagttag
    21301 tccagaatgg gctaactctt tttgattaat cattcttaca tgctgacagc aaagtcaggg
    21361 gcaaaccatt gaactgaacc aacttttacg ctttcgcctg gttgaggagc atggatatat
    21421 tgtcctccgc ctaaagcaat cgctacgtgg taagttccgc cttgtgaccc ccagaataac
    21481 aagtctccag cttttgcttg agaaactgaa atttttgttc cagctgattc ttgaggaact
    21541 gtccaaccac cgatgtcacg accagttact tgcatgtaaa catagcgtgt gaaccctgag
    21601 cagtcaaagc cacttggatc tttaccgccc caaacatatg gagtaccaat atatttgtaa
    21661 gcttctgcta cgattgctgc accattcaca cttccagaag gagctggagc aggtgttaca
    21721 ggttttggtt gttcaggtgt tggtgttggc gttggagccg gagtactact tcctgtgccg
    21781 tttcctgtat caacactttg atctgttgat ggtgttgtag gcgcaggtgt tgttgattct
    21841 tctgttg
//
LOCUS       DACECR010000045        21810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.045, whole genome shotgun sequence.
ACCESSION   DACECR010000045 DACECR010000000
VERSION     DACECR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.045"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            69..503
                     /locus_tag="IWO17_001555"
     CDS             69..503
                     /locus_tag="IWO17_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0290438.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(581..1114)
                     /locus_tag="IWO17_001556"
     CDS             complement(581..1114)
                     /locus_tag="IWO17_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ0290439.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1208..2386)
                     /gene="mutY"
                     /locus_tag="IWO17_001557"
     CDS             complement(1208..2386)
                     /gene="mutY"
                     /locus_tag="IWO17_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ0290440.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2379..3176)
                     /gene="recX"
                     /locus_tag="IWO17_001558"
     CDS             complement(2379..3176)
                     /gene="recX"
                     /locus_tag="IWO17_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ0290441.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3283..4665
                     /gene="rlmD"
                     /locus_tag="IWO17_001559"
     CDS             3283..4665
                     /gene="rlmD"
                     /locus_tag="IWO17_001559"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0290442.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4839..5417)
                     /locus_tag="IWO17_001560"
     CDS             complement(4839..5417)
                     /locus_tag="IWO17_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ0290443.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5739..6209
                     /locus_tag="IWO17_001561"
     CDS             5739..6209
                     /locus_tag="IWO17_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ0290444.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6317..7543)
                     /locus_tag="IWO17_001562"
     CDS             complement(6317..7543)
                     /locus_tag="IWO17_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ0290445.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8297..8497)
                     /locus_tag="IWO17_001563"
     CDS             complement(8297..8497)
                     /locus_tag="IWO17_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0290446.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8631..9239)
                     /locus_tag="IWO17_001564"
     CDS             complement(8631..9239)
                     /locus_tag="IWO17_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ0290447.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9324..9794)
                     /locus_tag="IWO17_001565"
     CDS             complement(9324..9794)
                     /locus_tag="IWO17_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ0290448.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9842..10756)
                     /locus_tag="IWO17_001566"
     CDS             complement(9842..10756)
                     /locus_tag="IWO17_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0290449.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10902..11705
                     /locus_tag="IWO17_001567"
     CDS             10902..11705
                     /locus_tag="IWO17_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ0290450.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11883..12830
                     /locus_tag="IWO17_001568"
     CDS             11883..12830
                     /locus_tag="IWO17_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0290451.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12898..13191)
                     /locus_tag="IWO17_001569"
     CDS             complement(12898..13191)
                     /locus_tag="IWO17_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ0290452.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(13219..13884)
                     /locus_tag="IWO17_001570"
     CDS             complement(13219..13884)
                     /locus_tag="IWO17_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ0290453.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(14024..14656)
                     /locus_tag="IWO17_001571"
     CDS             complement(14024..14656)
                     /locus_tag="IWO17_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0290454.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14663..15730)
                     /locus_tag="IWO17_001572"
     CDS             complement(14663..15730)
                     /locus_tag="IWO17_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0290455.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15727..16458)
                     /locus_tag="IWO17_001573"
     CDS             complement(15727..16458)
                     /locus_tag="IWO17_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ0290456.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16627..17106)
                     /gene="greA"
                     /locus_tag="IWO17_001574"
     CDS             complement(16627..17106)
                     /gene="greA"
                     /locus_tag="IWO17_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ0290457.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(17242..18417)
                     /gene="mltG"
                     /locus_tag="IWO17_001575"
     CDS             complement(17242..18417)
                     /gene="mltG"
                     /locus_tag="IWO17_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ0290458.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18621..19790
                     /locus_tag="IWO17_001576"
     CDS             18621..19790
                     /locus_tag="IWO17_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0290459.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(20057..21679)
                     /locus_tag="IWO17_001577"
     CDS             complement(20057..21679)
                     /locus_tag="IWO17_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290460.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     TFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         6750 a         4244 c         3449 g         7367 t
ORIGIN      
        1 gaaaacttgc catacttttt atttgtttta ttttccagta tactatacga aaggaaggtg
       61 gctagaccat gaaagttgta caaacaaaag atacaatgag caatatttac ttagatgctg
      121 tacggatcag gcatcaagtt tttgtagtcg aacagggtgt gcctttgtct cgagaaatag
      181 acaaagatga agcccattgt atccacttcg tcctgtactc agataaaaaa gaaccgcaag
      241 gaactgtacg tcttttgcct ttagaaaacg gaaaaatgaa attacagcgg atggcgattc
      301 tttctgaata cagacaccaa gggttaggaa aaatattgat tgaagaagca gaaaactttg
      361 ctaagaatca aggctataat acgattttat taggggcaca gtccacagct gaaacttttt
      421 acgaaaaact aggatataca gcttacggcg atccttttga agatgctggc atgcctcata
      481 tttatatgaa aaagacttta taaaaattta tttcgaaaaa agcaaaaaac agcaagcttt
      541 tgagaaaaat ctctcactgg cttactgttc ttttttgtgt ttacttacgc gacaattgct
      601 tgtaacggtt ataccaaata tcaatatagc cttccgagaa cgggccatct ccattattga
      661 tccagtcaac cagaatcttc acattttctt ttaggatgaa gtcaatatcg ttcgggtaat
      721 tcatcatttt gctgtgcatc tcatattcat cgacatccaa tagtcgtttc tcgccgtcag
      781 gaaatacctt gatatccaag tcatagtcga tatatttcaa ggcctcatcg tccagcaaaa
      841 aaggtgaagc tagattacaa taataggaaa cccccttttc tctgatcatt gctattacat
      901 tgaaccagta ttttttatga aaataaacaa tcgctggttc gcgcgttacc catcggcgtc
      961 catcagattc agtgactaat gtgtgatcat tcacgccgat catagagtgt tcgcttgttt
     1021 ttaataccat ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg taactttgga
     1081 tcgtcacaaa ctctccttct tttggaactc ccatagcaac ccttccttaa gcagcatttt
     1141 gtgctttgtg tttgggtgtt cattacaccg acatcataat tattatatca ataaaaatga
     1201 gctttgccta gaaaggattc ctattgtttc gatttttctc taactgatct accagtttca
     1261 tttggacctt aggaaaaacg accgaatcaa atgcatcagg cttcaaccac ttcgaagttt
     1321 tgttctcggt aaattcttgt tctgctccct cggttgcccg tccgtaaaaa aggagaacat
     1381 gccatttcag atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc tgccaaacaa
     1441 agagttcctg cttttcaaat gggagatttt gctcttcttc tgctactagg tcatcaaaca
     1501 gatcgatttc tggctttgtt tcccattctt ttttcaatcg ctcatactcc tcttgtgtca
     1561 cttccatcat tggaaatgtc cacatattcg ccagcaattt ttggctatct ctttcttcaa
     1621 aataatacgc tccagaatga ttttgaagag ctgcactgat ataataaacg tcttttggct
     1681 ttgctttttt tgtttttaca ggaaaactcg tttggattcc tcgtttgtta gctaaacaaa
     1741 aggcttggat cggacaagtc tcacactttg gagaagttgg tgtacagata gctgaaccta
     1801 gatccatcat tgcttggttg aattcacctg gatgcttttc atcaatgatt ttccgcatcg
     1861 cttcatcaaa gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg caaaacaatc
     1921 tgctgactac tcgcatcacg ttgccgtcaa ctgcaggttc tggaagtcca aatgcgatac
     1981 ttgcaatcgc gcctgtcgta tatggtccta ttcccttcaa tgagctaatt tcttcaggcg
     2041 tttgcggcat ttccccatca aattctgaca tgatctgttt agcagcagct tggatgttcc
     2101 gggctcttga gtaataacca aggccctccc atgcttttaa aagcttttct tctggagcat
     2161 ttgctagctc ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa tcgatcactg
     2221 tgtctactct ggtttgctga agcatgatct cagagatcca gatacgataa ggatcacgat
     2281 tatagcgcca aggcaggttt cttttttctt gttcatacca ttgaatgaac tgatcttgaa
     2341 attcttttgt ttctttatct gtccattttt cccattcttt attcactttc ctcttccttt
     2401 tccgtaatga atcgctggat catatccaaa tcaaatcctt tttgatataa gctttgcttt
     2461 acttttagat ttcttttttt aggttcaaac cttaaatttc ttcgccatag tttatctcct
     2521 tgtttgacta aataatcata ttctatttct tgatctgctt cttgcggaag atcagctatc
     2581 gcttgttgga tcacttcttg agaaaagcct ttcgtcatca atgtttgttg gatttttctc
     2641 aataattctt tttggctttt tccgtgattt tttccaaacg ctttttcggc tgtctgatgt
     2701 gcattttgaa cttggtcttc aaatgtatat tggctgagcg cttgttcaat gatttcaggt
     2761 ttcaaccctt tttgctgcat ctgctgcgct aatttctttg gtcctttgtc acttaagcgc
     2821 atattcgtgc gcacataact ttccccatag accaaatcat cgacaaggtc caattctttt
     2881 aagcgggcta cgattttatt tcgatcttct tgaaggattt ccttttcttt taagtatgtt
     2941 cggatttctt tttccgatcg caactgataa cttaagtaat tcattgctag gtgaaaaccg
     3001 aaatcagctc cgctactctt cttgatctct tggatagttt cttcgtctaa ttcccgtcct
     3061 tttaataatc gatagcgtac taatacatct tcagatactt gtaaagcttc tccttcagat
     3121 aaatctacgc gatagaaagg gcctttgcct ttactgattc tcgtaacggt taacataaac
     3181 atcctcctcg aacatatatt ctctatcata ccacagaaaa tccccgtaat caggtattat
     3241 atgttagaat aaaccacaga aaaaagaaaa agggagatac ttatggcaga aacaattgtc
     3301 aaaactggac aaacggttcc tttgaaaatc aagcgtttag gcataaatgg tgaaggaatc
     3361 ggttatttca aacggttgat catatttgtt ccatatgcac taccaaaaga agaagtacta
     3421 gtgaaaatca cgaaagcaac tcctcgctat gcagaaggag aattaataaa agtcaaaaaa
     3481 acaagcaaag atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg tggctgtcaa
     3541 ttacaacact tagcttatca agctcaattg gattttaaaa aggatctgct tttgcaagca
     3601 ttggaaaaat tcaaaccagc aggctttcga tcttatcaac ttttgccgac gatcgggatg
     3661 gatgagcctt ggcattatag aaacaaagca caatttcagt tacgaaaaaa taagcagact
     3721 caaaaaattg aagctggttt atacgaagca aactctcatc agttagtccc tataacagac
     3781 tgcttggttc aagaacctcg aacacaaaaa gtaatgaata ctgttgttca actattgaat
     3841 aaatttgatg cacccatcta tgatgaacgg acaaacagtg gaatcttccg cacaatcatg
     3901 gttagaatcg gtgttaaaac tggggagtta caagttgttt ttattactcg cagtaaaaaa
     3961 ttcccgcaaa aaaacgcaat gatccgtgag atcaatgaac gactgcccga agttgtttca
     4021 atcatgcaaa atgtccaggc acaaaaaacg tctctcgtga tgggggatga aacgatccac
     4081 ttatggggaa aagaagccat tgaagaacaa atcaatgaag tgactttcga tctttctcct
     4141 cgtgccttct ttcaactcaa tcctcagcag accgaattgc tttatggcga aggaatcaaa
     4201 gcattagatg tccagcctaa tgaaacgatc gtggatgctt attgcggagt tgggacgatc
     4261 ggcttaagtg tagcaaaaca agccaaagaa gtacggggaa tggataccgt tcctcaagca
     4321 atcgctgatg caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg atatgaagtc
     4381 gggacagcag agcaagttct gcctaaatgg ttgaatgaag gttttcagcc tgatggtatc
     4441 atcgtagatc caccacgtac gggtttggat aacgcattga tccaatcatt attgaaatac
     4501 cctccaaaaa aacttgttta tatctcctgt aatgtttcta cacttgccag agatctggtt
     4561 aatttaagta aaaagttcaa gattgaatat ttacagtccg tcgacatgtt tcctcagact
     4621 gcacgttgtg aagttgtcgt gaagatgacg agactcgctg actaaatgaa agaggttgtg
     4681 aaaaagtctg ccctgtatca agtaataaga acagccaaac aaaaatagct cttcatgttt
     4741 tatgaatact gtacttgtga ccgaagagtt aacaggagga cactatttat ttaaaaaaag
     4801 ggttgtgaca tagtttttgt cacagccctt tttgattatt attggatatt cacgtctatc
     4861 acttgataaa atgcatttgt cgtatcgctg atattccata ctgctaaaat cacattatat
     4921 ccttttcgat cctgcggaag tgtgatcgtt tggttgactg tttttggtgg aacagttgct
     4981 ttgtcgtcta tttttgtgat caattcaaaa tcactaaatt tcaaaggttg attaggattc
     5041 caagttggtt tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc tgttaaattc
     5101 caagtaatat ttaatgttcc tggattaacc atgtttttat gccatctgtt agatgtctgt
     5161 tcatctaaag gctcaaagcc gctgactcct gcactagcaa tttttccatc aatgaatgta
     5221 tttttggtag cttcgatact ttgcggttcc cattgtgctc ttccgacatt ttgatttaag
     5281 tttcctgctg ctgatgttcc taggtatgca cggcttcctg gagaagtaat ataaccatgc
     5341 gcaaaacctg gaatgctaaa accgaacaac cctaaactaa ctaacccaat aaacaataac
     5401 gtactaatct ttttcatgta gaaacctcct tcatttgata accacattgt aaacgcttta
     5461 ttgtattaac acaaacatta tgcttttata attttatgtt gaagtaaaaa aggtgaaaaa
     5521 agtaagatgc agattataac agaagtagac gagaaaagta caaatagaaa acatgagaat
     5581 caaatcatat gatctttttc aaaaacatca ttcatataaa caaatgttca actttttaat
     5641 aacaacaatt atcaattttt ttcttacttc acaaactaaa tacaggtctt gctgtgagcc
     5701 ttcatggtaa actaatatta tagaaaggag ttagtaaaat gaagatgata aaagatattc
     5761 gtttttgggt ttgtgttata ggaattgtca tattaggctt tttatcaggg ttgcttagcg
     5821 gaaatcccgg aaaatattac tattctttgc aacttccacc atttgcacca cctagctgga
     5881 tatttgggcc gatgtggacc ttactgtata tattgatggg tatttctctt tatttgttac
     5941 ttaatcacaa caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg attcagttca
     6001 tttttaattt tatatggtct gctctatttt ttaatttaag aaatattttc attgcagcaa
     6061 tcgatattac acttttagtt attttcttgt cagtgcttat gtatcagttg tggctgcatc
     6121 atcgcctggc aatgtggctc atgattcctt attatttatg ggtactattc gcaactctat
     6181 tgaattattc aatttatttt ttaaactaag cgctaaaaag agacgtcctg taatttaaca
     6241 catagaaaaa gtgccaccta agttagattt tttgtctaat ttaggtggca ctctttatta
     6301 accaaactat ttttttctag tacccacttg ttgttccata ctcattattc tggtcataac
     6361 cataaccatt ttgttcctgt ccagcgtaag gatcataact ttgagtatct tgacttggtg
     6421 tatagtactg acttgtatct tctgtttgct gataataggt attcggatct tggtactgat
     6481 catatgtgtt ttctgaatag gaagaataat cggtgtaccc ttcactattc gatccatcac
     6541 cgtttgtcga atcgccatct tccgaatcat caaagaacat cattgaagca ttctcatttt
     6601 tcaaattagg taatgtatcg cttgttttta tatttaattg tttcttcagt tcattttgga
     6661 tcgaaagaag atcatttttt cctaaaattt gatagtagat atcattgatc atttgtcctt
     6721 cgccttgcaa ctggtcttgt ttgattgttt caaacgccgg ggtgtagttc gtcgctacat
     6781 caagcatgtc atcccaatct aagtccgtcg tcacattgtt ctcaactgct tttaaaattt
     6841 ttctataatt gcttacgcca tctaaactta aaactttatt gacgattttc gtcactactt
     6901 cccgctgacg acgttgtcga ccgatatcgc cttctggatc ttcgtgtcgc attcgagaat
     6961 aggctaaacc tttttctcca ttcaatgttt gttcacctac tggtacatgg acaccatcca
     7021 aagtaaagtc aattttatta ttcactttga tgccaccgac cgcattgatc aaatcttcca
     7081 tgccatccaa attgatcgtt acataatgat caataggaat atcgagcagt tgctcaattg
     7141 aatccatggc catttcgaca ccaccaaatg cgtaggcatg atttaattta tctaccgttc
     7201 cataacctac gatattcgta taaatatctc ggtccaaact gatgatcgtt gatttttttt
     7261 gtttcggatt aactgttact accatcatcg tatcagaacg tccttgttcc gttcttccta
     7321 atccaccggt atctaaacct aatagcaaaa ccgaaaaagg ttctttgtca tcaatgctta
     7381 ctctctcaga acgtttggag gatattcgat tagagttttt agaaattttg tttactgttt
     7441 ggctcgcttc tcccataaat ctgattccgt atgctgataa tccggcaatc agcaaaacga
     7501 atacccctaa caaggttaat agaacttttt gccatcgctt cataatacac ctctgtttca
     7561 cattttctaa aatattgtct catataactg ttcaatcagc aaattcactt tttaatcata
     7621 agaaaacttt aaaaaacatt tttgtgtaac gattattatt ttttaaaatt tctcaaaatt
     7681 cttaactatc ataccatacc gtgaaaaaga tcgaaagacc gaatacacgc agttcgcaat
     7741 cgttttatct ttttttatgt attttttcca gaaaaagcga acaacaaggt attttccaaa
     7801 taatcgtgca gtaaaatgat cgcacatacc tttcccatct ttttaacagt ctttgcctac
     7861 cgttcagatt atcattcgat ttcacgacaa tttcttccta tgactggtcc ttttgttaaa
     7921 cccatcttct attcgtttta tttccagctc actgcatggc tcttaaaatg actcgaacta
     7981 gtgattgcat taccatcatc ttttaatgga tagatatgct cctcttcaaa gtaaaacgat
     8041 tttcttttaa agtaatagaa ttgacatggt aatgagtatg tatactagtg gtttcacaaa
     8101 tggtctttta ttcaacatcc aaaaactgaa atttctttaa aatccattac taacgaagaa
     8161 aatgttcctt gcttgataat tagttaggac atgtcaagtg aacaaatgca tttacttagc
     8221 caaaaaatct aaaaacttca taaatagaca aaaagacacg actctctaag aagtcgtgtc
     8281 tttattcaac aaattattat aatttagtca cttcagcagc ttgaggtccg cgtgcacctt
     8341 caacaatcgt gaattctact tcttgacctt cttctaggct cttgaagccg tctccttgaa
     8401 ttgctgagaa atgaacaaat acgtcatctc cgccgtctac tgtaataaac ccaaatcctt
     8461 tttcattgtt gaaccattta accgttccgt gttccataaa tgtatatcct ccaaaaaagt
     8521 tcttgcaagt aaccttgcaa gaacattata cccaatttag aaaacgctag caagctttcg
     8581 ctcttttatt ttccaaatta tttttaagac tgatatgacg gaggaaatgg ttattttgct
     8641 gcttcgaaac gtttgtttac ttcatcccaa ttaataacat tccagaatgc tgcaatataa
     8701 tctggtcgga catttttata tttcaaataa taagcatgtt cccaaacgtc caaacctaga
     8761 attggtgtct ttccttccat caatggagaa tcttgatttg cagtagaggt aatagctaat
     8821 tttccatttt ccattacaag ccaagcccat ccagaaccaa atcgtccagc ggctgctttt
     8881 ttgaattctt ctttaaaaga agaaaaatca ccaaaagctt cattaatcgc ttcttttatt
     8941 tctcctgtag gttcgccacc agcatttggt gccatgattt cccagaaaaa tgaatggtta
     9001 gcatgtccgc caccattgtt acgtacagct gtcttgatat ctgttggaat agcgtccata
     9061 tcagataata attcttctat tgttttttcg cctaattctg ggtatttctc aattgctgaa
     9121 tttaaattcg tcacataagt attgtgatgc ttatcatgat gcagatgcat cgtttcttcg
     9181 tcaatataag gttccaatgc gtcatatgca taaggtagat ctggtaatgt gtaagtcata
     9241 aaaaaattcc tcctcaatgt tttcttaata atgtctacat tacaaagagt aacaaagaga
     9301 aggaaattct tcaattgtta tgcctagtcg tctttttcac tttgacctac ttcttcttca
     9361 aaaacctcag tttctttaaa aataaacaac ttgctgaaaa tatagttcgc aaggataaca
     9421 ataatttgag tgatgatttt cgccacatac tcattgctgt tcataacatt gatcaacaat
     9481 accattgccc ccatgtccaa tccgtaagaa aggatacggt agaagaagaa cttaccgcat
     9541 tcaataagtg catgtgtaaa attcggtgat tttgaatgga atacccaaag tttgttcgtt
     9601 acaaaagcaa acaatacaga acaaaaccac gaaatagaat tggataaggc catacttaaa
     9661 tcaaaatatt ggcgtgcgag agcaaaacta acaaaattta caatcgtagc caaaccacca
     9721 aaaaagagat agactagtat ctcccaatat ccttttgctt ctaaatatcc ttttaactgt
     9781 cgatactgct tcatgagcca acctacctta ttaaaaattc ttatttatca tactctatat
     9841 ttcaataact ttcatcttta tttaaaaaaa ttaatttttt ttgatccttt gcctttgtct
     9901 ctgcttggat cgtcacatgt gtaataccat attttaattt taataaagaa attatacgag
     9961 gatatatctt ttcaatctcg cttagtagct gatcttcaaa atcaatatgt ccgtcgaaaa
    10021 taattatctt ttcgctatac tgccaaatat ggacatgatg aacatccttt accccatcga
    10081 ttgcttcaat ttcttgttcc attttttcat agtcgatagc aggtgcactt tgtatcaaaa
    10141 tagcggcagc ttttttgatc accattatcg cttctatcaa tatatatatc gaaatgatca
    10201 gtgtaataac tggatcgatc cagtagatat cgaaaaaacg gatcaaaatt gctccgataa
    10261 tcacacttac cgaagacagt gtatcactta acaaatgcaa ataactagat tttatattca
    10321 aactttcatc tgcttcttgt cgtaataata atactgagaa caaattcgcg agcaatccaa
    10381 taactgctac tgttagcatc aaatgactat ttatcttttc tggtgaagtg aaccgcttga
    10441 atgcttcaac ggttaaaaac aaggataata tgatcaatat gaatgagttg atgaaagcac
    10501 tcaatatttc taaacgctgg tagccatacg tacgtttttt atttgcttct tttcgggcca
    10561 ttttttgtgc cccgtaagaa aaaataattg ccaatgtatc actaaggtta tggaaagcat
    10621 ctgaaacaag agacaaactc cctgacataa tccctccaat aaattctgat aaagtgatca
    10681 ccgcattcaa aaccactact ccaaatagtt tttttgatga tctctgtggc tgtgtaggca
    10741 ctattccttc ctccatttta tttatattgg atagtcttta gacaagaata acattttttt
    10801 gatatttcac caatcattca gctgtttcct taaagcactt gtatttcact tgatacttcg
    10861 ctagaattgc aagtgatgac actactagat gaggagtact tatgacaaca aaacaattaa
    10921 ttatcagttc ctttatacga ttcaaagacc taaaggaggc gagaaatgtc ccattttgga
    10981 aagccattgt ctatctttta gttttgagta taattatggc tttgccaatc tcttttcaag
    11041 tttttcaagt cttaaacaac attcaattgg atgggcaaaa gattgctact cgcattcctg
    11101 attttacaat ccaaaacggg cagcttcaaa ccgaagaaaa agaaggattc atttaccaaa
    11161 caaactccat tatcttcaca tttgatccag agggaaaacg aacagaacag gacatctcta
    11221 ctgatttgat ggggaatttt gttagtgtcg ggatgttaaa aaacaaactt atcatagctc
    11281 ttcctaatac aggcacgact tctgctttgt tgaacaataa ccaattagaa cttccctaca
    11341 ccaatgaatc tttaaaaaac ttaacaggta agcagctccg ttctttttta agtgaagcat
    11401 ctataccact ttggataaaa gtcctgacat ttatattttc tatttatcct agttttctga
    11461 atctgctgat cactttgcta tttaccaaca ttgctgcttt tctctatgca cgctttcgac
    11521 taacaaaagc tacttttctt gattgtttaa aaacattgat ctattcaatt tcattgccta
    11581 ctataattgc aacaatcttg atggtctttc ttccttcttt tgacactagc gccttcattg
    11641 cttttgcagg aatctttatt tttgctcaag ctgtcaaagg atgggcaaaa atatcgatca
    11701 attaagaaat aaagacaact cgttatttct acttcaatag acccttttag aggttgtgac
    11761 atttttttgt cacagcccct tttttatttg aaaaatattt ctttctcaaa aaacaagcaa
    11821 ggcgcttcaa agttcttctt gtatgcgcta cacttagagt aaagaaaggc gggaaattaa
    11881 ctatgtcaga aagaatcatt tatgcagatc gtccatttcg tgatgaattt atcgaagaaa
    11941 tccatactat agcgcctggt tatatcttta aaacagaatt acaaccagag gatctacaaa
    12001 atgtagagat cagtttgggc tggaactcag actaccaaga aaaactatta gcctcttcta
    12061 ccttgaaatg ggtccaatct atctcagctg gtgttgatca gctacccctt gagacttttt
    12121 caaagaaaca tattttactt tcaaatggta gtggcatcca cagtgaatcg attacggagc
    12181 atgtcttagg aatcatcctt gggtattctc gtggtctttt tcaagcacaa aaggcacaga
    12241 gtgacatgaa atggttaggt tcatcggtcc attaccaatc tatccaaggg aaaaaaatac
    12301 taatcattgg aactggccat attggaaaaa tgattgcaaa aaaatgcaaa gcattagaca
    12361 tgaactgcta tggaatcaat acatccggtc atccagcaga aggaatggaa aagacttttt
    12421 ctttaaatca actaaaggaa gttcttcctg aagtagattt cgtagtcaat atcttacctc
    12481 taacggagga aacaactggg ctctttgatc agcatatgtt tgaacacttt gatccaaaat
    12541 ctgtattcat caatgttggc cgtggtgctt ctgttaagac acaagatctg gtgcaggctt
    12601 taaataacca gcaactttcc tttgcagcat tagacgtttt tgaggaagag cctcttccac
    12661 aagatcatcc tctttggaaa atggacaatg tactgattac ttcccacatt gcaggtttga
    12721 cacctgattt tcaaaagaaa ttaatggcta tttttttaac caatctgaaa agctactttg
    12781 ccacccatga gcttcaaacc aatcaagtaa aattgtctgc aggatattaa tagctgaaaa
    12841 caatattttc aaaagaaaaa agagaaggtg acaaaaattg tgtcaccctc tcttttttta
    12901 ttgtttttta aagtgatagg ttggctcctc caggttggga tcataatcaa caagtaaatc
    12961 atatccttta aagaaccatt catctgcttc ctcgataaaa aaaagtgtcc catcaatttc
    13021 cactttcttt acaggatgtt ctggctgttc aactgacatt ccaatcgaaa aaccatcatg
    13081 aacctctgtt ttgccgtaga cttttccaaa aaaacgtatt ccgtaagttt ctggcagatc
    13141 aatttctgag tcaaaccact tttgtgcttt tggcgttacc gttaatttca tcccaacaac
    13201 ctactttctc ttttctttct attgattcat cttatcgtct agaatatcca catcgcctgt
    13261 ctctccaata accagtagat tatcattttc acgaacatat tcatctgctg caggagaagc
    13321 gataaccgtt tgatctactc ttcgtatggc gactactgtt aagtcaaaac gttgcctgaa
    13381 atttaattca gctaatgttt tgttgaaaaa tttaggattg gagactcgga tttctgctaa
    13441 agaatattta tctgacaact ctaggtaatc taaaatattc tttgacacta aattatgcgc
    13501 aatccttatt cccatgtctc gctcgggatg gacgacgcga tcagctccga tcttttcaag
    13561 tactctggca tgatactcat tttgggcttt tgctaaaata tttggtacgc ccatttcctt
    13621 taccatcaga gtaactaaga tactggcttg aatatcttct ccaatagcga caattacatg
    13681 atcgaaattt cttatgccta atgatcgaag tgtcatttca tcctgtgcat ttgccacaac
    13741 agcatgagtg gctatattca tatattcatt gacccgatct tcattactat caatagccag
    13801 tacttcttga ccagcttcga tcagtgttcg gcagatactt ccgccaaatc gtccaagacc
    13861 tacaattgca aaattttgtt tcaccgtttt cctcctttag tatcttcttt tctcagtata
    13921 acaaagcctt tacaaaaaca aaagcaagca tcattattgt aaaaaagcca agaaaaataa
    13981 tagttatttt ctcagctttt gattatcctc tttatcatcg tttttatttc accaaaccat
    14041 gtttgaatgc atagatagcg gcttgtgtac ggtcttccac ttctagctta gccaatatat
    14101 tggaaacatg tgtttttact gttttcagtg taataaacaa ttcgtccgct atttcctgat
    14161 tactttttcc ttcagaaatc agcattaaaa tttcattttc ccgattcgtt agttcttcat
    14221 gtaatacagg ctcatttcgc cgactcattt tttccatcat ttttgttgtc acttctggtt
    14281 ctaatactcg ctcgccacgt tgcgttgccc ttatagcatc agcaatttca tgtgcggtag
    14341 aggtcttcaa caaataccct gctgcacctg cttcaatggc aggataaact ttttcgtcat
    14401 caataaaact ggttacgata atgattttag cttctggcca atcttttaag attgcttttg
    14461 ttgattcgat accatccatt tcttccatga ctaagtccat taaaatgacg tccggtcgaa
    14521 gtgtcatcgc cttctcatag ccttgtcgtc cattttcagc ttctccaatt acttcaatgt
    14581 cttcttgaat agataaataa gaagagacgc ctaagcgcac catttcatgg tcatctacta
    14641 ataaaacttt tatcatcctt atctatgcct ccttcatcaa agggaccttg atttctacac
    14701 ttgtaccttg acctttaaaa ctaatgattt ttactgttcc accaatacca gctactcgtt
    14761 ctttgatatt gttcaatcca taacttcccg ttttcgtttc attcatatta aaccctgttc
    14821 cgtcgtcaat aatacgtaac aaaagattat tgtctatttt gtgcaaatat acctctaatt
    14881 cattggcttt agcatgtctt aatgtatttg ataacaattc ttgaacgatt cggaacaagt
    14941 gatcctcaat ggaactagat agttttacat cttctacatc ccatttcagc gaaatctgga
    15001 ttttattttg caattccttc aataactgct cgataccttg ctttaaactt ttctcttcta
    15061 aattgactgg acgtagatga agaagcagcg cacgcatctc agactgggaa gcgttgatga
    15121 tctctgctac cattttcaac tgcttgcgaa acatttctgg tgtctcgctt ttttctgctt
    15181 gctccgtcaa tgccgacatc atcatcattg ctgcaaacaa ttgttgcgaa actgaatcat
    15241 gcagctcacg ggctaatcga tgtctttcta attctaaaat ttcttctttg gtttgtccat
    15301 ctacatattg gggacggctc gttacaattt gtaattcact agatacttca atcatttttt
    15361 cttttatctt agtgatttcc ttatcaatat ctttgatgta taaatcgtcc gatgcacttg
    15421 ggatcggaag aagcaacaat ttggattcat aattgccaga agaaagtgca cgcagctttt
    15481 cttcgatttt cccgtatttt gtcttttgaa taagtgataa taatagaaag acaattaatc
    15541 ctacacctaa ggaaatggct aacaaatgga agataagcgg aacatataat aaacgtgcac
    15601 gcagtaattc tccccaccaa tgactttgat tgctggcata aaaataagtg aaaagtgaaa
    15661 atagaataat aaggaaagcc gcaatccccg aataaacagc tagcatcgct ctggatattt
    15721 ttcccatcat attcgtatca cctcgacatc tccaatcaag gtattagtaa taatcttcaa
    15781 tctacgaggg ttttcatcat aatcactgct gtagattttg atttgttcat ttttcaaaga
    15841 aaattgctct tcttcaaaca agacatttcc tactaatgtc gcatgctcta atcggatggc
    15901 tacgcccaaa ggtactaaga tccgtgttct gcctatccct tttcttacaa tcacaatatt
    15961 atcatctttt ggcaaaagcg tattgcccaa atctatgata gtatcaccag aaatgacggc
    16021 gatgttgata tcgtcccatt catagacgtc gtttccaata cgttggttac cgaataattg
    16081 ctgtcttttc cgctcattat tatgtgtttt taattgctct gtttggatca tcatgatctg
    16141 tttctttcgc caaaaagcat ttttggttaa atctatccct gaaatctcta cacctttcag
    16201 accaataaat aaaacggcaa atacagccat catccataaa gcaggactgt tgactaagct
    16261 aaaaaaaatg accaaacttc ctaatatcaa ttgaaaattt tgaaatttag tacgaggatg
    16321 tttgcgcata gctagatata tattgaaaat accgaatatc actaaaagca aaagtccagt
    16381 attgtttacg atttgccaaa cagcaaataa cagcagtagt gcttcaacta ccacaaaaaa
    16441 acgccaagaa ctattcatcg ctctttctcc ctcctgacac attctcattt catcatacct
    16501 atttttttaa ctgttggcta taggacttta gatgtaagat cgtttgttga aatctaaaat
    16561 acacaaagag aaaaagatct agagtgtatc atcagtaaga tgaactctct agatctttca
    16621 gtcagactac gcttgttcta ctttgacgat tttaactaac atatcgccac ctggagtagc
    16681 aatagttact tcatcatttt tacgtttgcc aatcaatgct tgcgcaatag gagaatcatt
    16741 ggaaattttg ccagaaaacg gatctgcttc agcgcttccg acaatcgtgt actcttcttc
    16801 ttctccatct ggtagttcga tgaatgtaac tgttttacca attgatacct catctgcatc
    16861 tacaccgtcg ttatcgatga tttgagcaaa acgaatcata ttttcaattg ttgtgattct
    16921 tccttcaaca aatgcttgtt catctttcgc tgattcatat tcagagtttt cggaaagatc
    16981 tccgaaaccg cgggcaattt tgattcgttc aatgatttct ccgcgtttga ctgtttttag
    17041 ttcttctaat tctttttcta atttttcttt accttctaga gtcattggat aaactttctc
    17101 taccatgtgt aacgtctcct tttttcgata aaataccgca aaaggtaatc ttttgaggaa
    17161 tatagtccaa aaaagattac cacgcttttc ttcaaatgta aatgtttttc taaaaatgat
    17221 caatgtctag agcctgtttt tctaagaatt atttacatat ttttccacta attccatatg
    17281 ttcttcataa gttttagcga aataaacatt tccagtgctc gtatctgcta cgaaataata
    17341 atagtcattt tctgctggtt ctaatactgc cttgattgcc tcttcgcttg gattgtcaaa
    17401 aggtccaggt ccatagccgg tattggtata caaattatat ggtgaatcta cagctgtatc
    17461 ttcatacgtc actaattctt tgtgttcacc taatgcgtat aaaacagaga tatctgactg
    17521 aagtggcata tttgctttga ttcggttaaa gaagacttgt gcaatgtttt tacgatcatt
    17581 ttcttttacg ccttcttttt caactagaga agctagagtt aaaacttctt gtacagtcaa
    17641 atttttctgc ttgatagctg aataatagtt ttgcatgacc gtattcgtct tgttcaccat
    17701 ttcaatcact agatccttaa gcgaagtact cgtgtaataa tcatatgtgg ctgggaataa
    17761 gtaaccttcc aaacggtatt ttgtgttctc agctttagat gcactttcca acagttctgg
    17821 gaatttttgg tgcaactcgt taaagaaagt ctcatcattc actaaatcta aaaattcttt
    17881 cttgtcttta ccagttacct tcgctacccg ttcagcaatc tgatcgatat cgtacccttc
    17941 tggaatggca attttcccat ctgcaacttt tgttggctca gaagtaccgc cttcttgcaa
    18001 ctgtttgcca atctcgtcaa gagtcatatt aggcgctaac tgataatagc cagcttgaaa
    18061 gccagttaaa tttttaaatt ttgtataata attgaaaaca atcccgcttt taataatatt
    18121 gtccttctcc aaaatctcac caatctgctt gttagaagag ccactcggga tctcgacttg
    18181 gactaattgg tcatctgatt tatctaaagg cttcaacccg ctatctacat aacgatacac
    18241 ggtaaatcca agaaatccgc caataatcaa taatgtaaga gcaatcacta aaatgatctt
    18301 cctcacaatc ttatcttctt ttttacgcag atttctccgc ttgtcgatta tatctgctgt
    18361 agctgcctta ctggcttctt ttttatcttt acttgggtgt tgtttgtttc ctgacaaaac
    18421 aactcctcct tactatcaag ttcgtaactt tattcattat acatgatatt tgaattggcg
    18481 tgaatgattt tatttaaata ttctatcaaa aatgtaagta gtaaatccat tactataaga
    18541 ttaaaataaa tgttatcaag atacaagcta ttttagcttt attctttctg taaaaaagaa
    18601 aggagactaa ataaactatt atgaaaaaag gaaaacatgc taaaaaaaga aaatattcat
    18661 ttttaatgat cgttgtcgtg ttgtttagcc ttagtgcgtt ctctacagga actattcttg
    18721 gatggcaatt ttacaataat gaacactctc agatagttgc ccatgctcaa tcagctcaaa
    18781 aaaagttgat actgactacc aatgaacaac agctcgttga aaaaatcgat caactattaa
    18841 gaaaaaatga ttatattggc tcaatttacg taaagaaaaa taatcgcatc atcatggaaa
    18901 aaggctatgg ctatgcgaat aaactcacgc aacaaccaaa tgatccttct ctttattatc
    18961 agattggttc ggttcaaaaa gcattgacta ctcttttgat tttaaaacaa gtcgagcgtg
    19021 ggcacctttc tttagatact aagctggctc aattctatcc acaaattgct ggcagccagc
    19081 aaattactat ccgtgacttg ctgtatatgc gctcaggctt gcaccgaact gcttctccaa
    19141 ccataccgat gactgatgaa gaagtcgttc aatttgcgat tcagcactta aaatttacag
    19201 attatcagac ctctcattat gattcattga actttaccat attgactgga attttgatca
    19261 aattaacaaa tcaaccgtat gaaaaattat tgaccgatga tatcatcgaa cctttaggat
    19321 tacagcatac aaaattttat gaccaagtca aaaattcatc caatcatgca ctatcctatc
    19381 aaatgtctaa agaggatgat tactttaaac cactgacaga atctgaaaca gagatccata
    19441 acgaattagg gactggtaat atcagtatgt ccgtctatga tttaaatacg ctgtttacac
    19501 aagttttatc tggaaaacta gtttccaaag acttactctt ctcattatgg cgtgaaaatg
    19561 cgaatggaca tccatatagt ggtggggtat atagcggaga taattatatc ctgtcgcaag
    19621 gaaatatcga ctcttttgat acatctgcta tatttagcaa agattcacag gatgctatca
    19681 tcatggaaag caatataaaa gctgacaaaa ctatcaaact gcctactgct gatctaagaa
    19741 accaaatcta tgacctgatg gaaggaacta ctaagttggc aaatagctga tatgatgaaa
    19801 agatagcaaa tactacttct ttgaatagca tttgcttaaa aaaagtactc gactaaacgt
    19861 aggaaagagt gtgggacaaa tctaaaaatc aatttttgct acagacttta attccaaata
    19921 aacagaacag aaagaggtgt tcttctttct gttctgtcca aattattcta agatttctct
    19981 ttaccaacat tatttgacaa agggcctatt aaatctaaaa tagtatcttt agattgctaa
    20041 aatatgtgct agcaaatcat tcatggcttg ttcttatttt taattttatg caataaaaaa
    20101 gaagaacaat tagcgtgatt atagaaataa ataatgagat tttagaaaaa aatgtgtatg
    20161 aatacctgat tgttacttta tttattccac tttcactttt tactgtaaca aaaggacctc
    20221 cttgttttag tactgtagca ttttttgaat taacagtaac cgttaatcct ttataccgga
    20281 atacaggtaa aactattgtt ttgccttttg aagatttaaa cgtaaatgta atttcatctt
    20341 tatttggaga aaagctacta taaataactt gaccatcttc tgtaacttga tggtttttca
    20401 aaagcaattg atctttttta tagcctatag gtacataatc aaaattctca aattcttcaa
    20461 gtttattttc catttgttca ttagaataag ttccttgaaa attcatttct ttcaccatgt
    20521 taactactga tgaataattt aaaattgaaa tagctaatat taataatagt ataaaatttc
    20581 ttctccttgt ataagaaaat ttttccaact gtggagaaat aaatagagat actgaaaaag
    20641 atatgaacaa tgttgaaaat atcataaaac gccaaggagc ttgaataata tttaaaggag
    20701 tattttgtaa ggcaaaccat ggaaatattt ttgtacaaag tagaatagat attataccca
    20761 ttatataagt gattttagat aagattccta attttttaaa gttaaatgtt agtataatta
    20821 aacaaataag caaagttaag ccaataccaa atgatagtaa ttctgaatct atacttccaa
    20881 tcaaaaactc tttacccaga taaattgctc ttttttgtaa tatatccaca taaggtctat
    20941 tcagatcttg atacaatgtc tgctcaatca taggacctat aaacgctagt gaaagaagta
    21001 aagtaactat agcagctttt attaatttaa taattaagaa gatttctatt ttctttttcc
    21061 gataaaattg aataattaaa gcaattataa tcattaccat agtcatagct acagaaagaa
    21121 catgagtgta aattaataaa gtcatgctaa tcgccaacat aacccaagat tctttttttg
    21181 agtcgtaaag ctttataaac ccgagtaaaa taagaggaag tattgacatt gcgagtaatt
    21241 cccccattgc ccctcgaaga aatatttcta cactcctaaa agaagatgtt gtatatataa
    21301 tagcgaaaaa ggagctagta acatgttttt tggtaatttc atacattaca taatgacata
    21361 taaatagagt aatgagcgta acaatataaa gaaaaaggtt atagcctatt gctaaattag
    21421 taaacttgat caataaaaat aaaggatata gagttaacca tgggtaaaaa ttatttactt
    21481 gcatgccata tgatctaaac gtttcaaaat taatagggga gctaaaaata gattctaaac
    21541 ttaacgcacg attaatgtga aatgccgtat cataaaagcc aaatatttcc atgtttttaa
    21601 atgctggaaa taaatataat aaactaacaa atgcataaac tataaatatt tttacagaaa
    21661 aaattttttt caaattcata taccacctca tgttttttta tattacatat agtatcattt
    21721 atttttttaa aacactagat tagtcactat ataaaatgtg attaatttta ttattaataa
    21781 aatatttttt atattacaaa agtagtgttt
//
LOCUS       DACECR010000046        20496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.046, whole genome shotgun sequence.
ACCESSION   DACECR010000046 DACECR010000000
VERSION     DACECR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.046"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(13..996)
                     /locus_tag="IWO17_001578"
     CDS             complement(13..996)
                     /locus_tag="IWO17_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ0290461.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1001..1648)
                     /locus_tag="IWO17_001579"
     CDS             complement(1001..1648)
                     /locus_tag="IWO17_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0290462.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1733..2365)
                     /locus_tag="IWO17_001580"
     CDS             complement(1733..2365)
                     /locus_tag="IWO17_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0290463.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="IWO17_001581"
     CDS             complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="IWO17_001581"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ0290464.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(2929..3666)
                     /locus_tag="IWO17_001582"
     CDS             complement(2929..3666)
                     /locus_tag="IWO17_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ0290465.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3663..4652)
                     /locus_tag="IWO17_001583"
     CDS             complement(3663..4652)
                     /locus_tag="IWO17_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0290466.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            4910..5452
                     /locus_tag="IWO17_001584"
     CDS             4910..5452
                     /locus_tag="IWO17_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0290467.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5469..6554
                     /locus_tag="IWO17_001585"
     CDS             5469..6554
                     /locus_tag="IWO17_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0290468.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6566..7372
                     /locus_tag="IWO17_001586"
     CDS             6566..7372
                     /locus_tag="IWO17_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0290469.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7373..8200
                     /locus_tag="IWO17_001587"
     CDS             7373..8200
                     /locus_tag="IWO17_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0290470.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8197..9270
                     /locus_tag="IWO17_001588"
     CDS             8197..9270
                     /locus_tag="IWO17_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0290471.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9698..12394
                     /locus_tag="IWO17_001589"
     CDS             9698..12394
                     /locus_tag="IWO17_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0290472.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            12471..12959
                     /locus_tag="IWO17_001590"
     CDS             12471..12959
                     /locus_tag="IWO17_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0290473.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            12971..13465
                     /locus_tag="IWO17_001591"
     CDS             12971..13465
                     /locus_tag="IWO17_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0290474.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            13486..14295
                     /locus_tag="IWO17_001592"
     CDS             13486..14295
                     /locus_tag="IWO17_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0290475.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            14292..15113
                     /locus_tag="IWO17_001593"
     CDS             14292..15113
                     /locus_tag="IWO17_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0290476.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            15164..17263
                     /locus_tag="IWO17_001594"
     CDS             15164..17263
                     /locus_tag="IWO17_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ0290477.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            17260..18378
                     /locus_tag="IWO17_001595"
     CDS             17260..18378
                     /locus_tag="IWO17_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ0290478.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            18417..19295
                     /locus_tag="IWO17_001596"
     CDS             18417..19295
                     /locus_tag="IWO17_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0290479.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            19486..19638
                     /locus_tag="IWO17_001597"
     CDS             19486..19638
                     /locus_tag="IWO17_001597"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290480.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         6279 a         3526 c         4361 g         6330 t
ORIGIN      
        1 atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt tgtttgcttc
       61 catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa tgattggccg
      121 aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg cgagctctgc
      181 tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac gtttcccaat
      241 caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa tcaagaccca
      301 acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc cgatgaaatc
      361 caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt gaaagtcttg
      421 ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga gtttttcttc
      481 tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt ggatcgctgc
      541 tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt gtaattttcc
      601 aagccagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag cagcagaaac
      661 atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag catattcggc
      721 ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag tggagatatc
      781 acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt agggaggttt
      841 caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg tatggacttt
      901 gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt ctaatacatc
      961 tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc tttgcccatt
     1021 cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc gtacgagaaa
     1081 tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga gtgatcgttt
     1141 ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca tcgttagtca
     1201 atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat tcatctgcat
     1261 aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt tcatctgcat
     1321 aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg atcactgttt
     1381 tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg gcagcaaaaa
     1441 tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa gttaaatcaa
     1501 aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca tctctcaccc
     1561 ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg ccaatttgtc
     1621 cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca aatctttcca
     1681 ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac tatcagttag
     1741 taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca gtcgcaagac
     1801 ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata gctgaataaa
     1861 cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa aagtagatca
     1921 tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag aataaagaaa
     1981 cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa taaggaacca
     2041 ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc tttccagctt ccatgttctg
     2101 ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg ataactgtgc
     2161 tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt cttggtccta
     2221 aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt ggaccgatag
     2281 ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac atcgcagtta
     2341 atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt ctttcattaa
     2401 gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct tttccaaaat
     2461 ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca gatctcaatg
     2521 tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg gcaggggcaa
     2581 tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga tcgtctgcaa
     2641 tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga aacaagtcag
     2701 cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc atgacgtctg
     2761 tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa tgtgtgctgc
     2821 ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt gtaagatcag
     2881 cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc attttgattc
     2941 ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt gagcttcttc
     3001 cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga tttccgtcaa
     3061 atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg cgactgccag
     3121 aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg cttttggctg
     3181 ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca caagatgctc
     3241 ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg caaaccaatt
     3301 gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt cttctgaaaa
     3361 actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat cgtagtgttc
     3421 ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga tcgtataaat
     3481 cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt aatcgattgt
     3541 ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc ctgtcgaatg
     3601 atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg tgattagaat
     3661 cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc agcaagtagt
     3721 ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag ttcatctgca
     3781 atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg tacattgaca
     3841 gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt cgcgtttagt
     3901 ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat caatggaaga
     3961 agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg ctgttttcct
     4021 aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa agcacctaga
     4081 ctttttagcc atgtgacaga ctcttctttt ctggaaaagg caatgattga ctggaatcct
     4141 agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc agtaacgaca
     4201 acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa catagcagta
     4261 aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg tagaggaact
     4321 aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt gacgccaagc
     4381 ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt actttggacg
     4441 acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac ctgaccagac
     4501 tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt taccaagact
     4561 tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa aacaggttcc
     4621 tttattacct gaaaagatcg aaaagcgttc atttgtgaga cactcctttt aaaattttct
     4681 tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt agacaaaaat
     4741 taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa gaaatattca
     4801 gcaaaaaaaa ttttacaaat ggttgccttt ttcaataaat ttcgtatact aatacgagtg
     4861 gtagtaaaca ggaagtttac tattagaaaa cttcgattcg gaggaaatta tggaaattgg
     4921 tgagaagtta agaaatttgc gcatccaaaa gaatttgaca caagaagaac taggcgaacg
     4981 cacagatttg tcaaaagggt atatttccca gttggaacga gatttaagtt caccatcaat
     5041 ggaaacattc ttttctattc ttgaagtctt aggtgtgact ccagaagaat tttttcatca
     5101 agaagcagcg aatctgcaag tggtttattc aaaagaagat catacggttt atttcgatga
     5161 agaaaacggg tatgaattgg aatggctcgt ggctgattcg aatgaaaaag aaatggagcc
     5221 tgtcttgctg acttttgaag gtgcaggaga atataagacg tttgaacctt cattatctga
     5281 aacgtttatt tttgtattag aaggggaatt agagttaagc ttaggagaga cggtctatgt
     5341 ggcaaaagca ggacagtcga tctattatca agcgacaaaa caacatcaat taaggaatca
     5401 tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa tcatatttgt aatatagggg
     5461 gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt agtgaaacgc tatgatgatg
     5521 aaacagtatt aaaaaaagta agttttgaga tcgaacaagg aaaattttat acactgcttg
     5581 gtccttccgg ctgtggtaaa acaacgatcc tacgtattat agctggattc accgaagcta
     5641 cagaagggga catctatttt gaagggaaac ggttgaatga tttgccagct aataagcgac
     5701 aagtaaatac agtcttccaa gattatgcac tttttcctca tatgaatgtt tttgataacg
     5761 ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga tatcgaaaag aaggtaaaag
     5821 atgctttgcg tatggtccag cttcctggat acgaaaaacg ggagattagt gaaatgtctg
     5881 gtggtcaaag acagcgtgta gcgattgcac gagcaattgt aaatgaaccg aaagtattgc
     5941 tattagatga accgttatcg gcgcttgatt tgaaactacg tacagatatg cagtatgaac
     6001 tgcgggatct gcaacaacga ttaggaatca cgtttatttt tgttactcat gaccaagaag
     6061 aagcgttggc aatgagtgat gagatttttg tcatgaataa agggaaaatc gtccaaagtg
     6121 gtacacccgt agatatttat gatgagccaa tcaatcattt cgttgctgat tttgttgggg
     6181 agagcaatat cgtggacggt gtaatgatcg aagataatct agttgagttt gttggtaagc
     6241 aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc agttgaagtt gttttacgcc
     6301 cagaggattt gacgatcacg acaccagata aaggaaaact agtcgtaacg gtcgatactc
     6361 agctgtttcg tggtgtccat tatgaaatca tctgttatga tgaacagcaa aatgaatgga
     6421 tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt aggattggcc tttgagccag
     6481 aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga attcgatgca cgtcttgaca
     6541 gctacgaaga atagggggga caagaatgaa aacaatgcgc aggatctatt ccgtccctta
     6601 tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta atgatcattt atcaatcttt
     6661 ttttgatatg aatgggcaat tcacgctgaa taattatcta tcttatttta cgtcggggac
     6721 atcgctttct atgacgatca attctgtatg gtatgcattt ttgatcacgt ttttcacgct
     6781 gatcatcagt tacccgactg cttatttttt aacaaaactg aagcacaagc aattatggct
     6841 gatgctgatt attttgccta catgggtgaa tctgctgctt aaagcttacg catttattgg
     6901 gattttcagt gtgcacggca gcgtgaatca tttctttgat tttatcggga tcgggccgaa
     6961 acagatatta ttcacagact tcagttttct gttcgtggca acgtatatcg agattccatt
     7021 tatgattatg ccaattttca atgcattgga agagatgaat ccttctttga tcagtgctag
     7081 tagagattta ggcgcgaata gctttgagac cttccgacga gtgatttttc cgttatcatt
     7141 aaatggtgtg aaaagtggtg tccaagcagt atttattcct tctctttctt tattcatgct
     7201 gactcgtctg attggcggaa atcgggtgat cactttagga acggctatcg aacagcattt
     7261 cttggttaca caaaactggg ggatgggttc tacaatcggt gtgattttaa tcattgctat
     7321 gttcatcgtg atgctcctga caggcgagaa gaaaaaagga ggtcgctcat aaatgaaaaa
     7381 atttaaatgg tcgtatcttt atttagggat cgtcttttta ttcttatatg caccgatctt
     7441 ctatctgatt ttttattcct tcaatgaagg aggaacgatg aacagcttta caggatttac
     7501 ttgggaaaat tatgaagctg tttttgaaga tacacgtttg atcattatcg tattgaatac
     7561 gttcatgctg gctttcttat ctgcattgat cgccacgatc atcgggacat ttggggctat
     7621 gggaatctat tacacgaaaa aacgacgtgc acggacagcg ttgttgagca tgaataacat
     7681 cttattagtc tctcctgatg ttattatcgg tgctagtttc ttgattttct ttacactact
     7741 aggattcgga ttaggttttc agagtgtatt gctttcccat attgctttca gcattccaat
     7801 cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac gattcaatgg tcgatgcggc
     7861 tagagacctt ggtgctaata atttccaagt agtcaaaaat atcattttgc cttatctttc
     7921 tccaggaatc atagctggat attttatggc atttacctat tctcttgatg attttgcggt
     7981 tacatttttc gtcacaggaa atggctttac gacgctctca gtagagatct attctcgtgc
     8041 aagacaagga atcagtttag aaatcaatgc gctaagtgcg ttagttttct tgttctccat
     8101 gatattggtc attggatact actttattag taaagaaaac cgtccaaaac ggctgaagaa
     8161 aaacagaaga gtacgcgagg gggtggcacg gctgcgatga aaaaattaca atctctgttt
     8221 ttaggtattc tcatcattat tgcggtgctg ctgcttggtg ttcgtcaact tgaaaaaaca
     8281 agtggcatga gtggagcaaa ggtacttacc atctataact ggggagatta tatcgatcct
     8341 agtttgatca cgaaatttga aaaagaatat ggatacaaag tgaattatga aacatttgat
     8401 tctaatgaag cgatgttcac aaaaatccag caaggtggga caaactacga catcgctata
     8461 ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac tagtacttcc attagatcat
     8521 tcgaagatca aaggattaaa taatattgat ccacgatttt tagacttgga ttttgatcca
     8581 gataatcagt attcgattcc atatttttgg ggaactttag ggatcgtata taacgataaa
     8641 ttctttgatg caaatcaaat taagcattgg gatgatttgt ggaagccgga attaaaagac
     8701 agtttgatgc tgattgacgg tgcacgagaa gttatggggc tttctttgaa tagtttaggc
     8761 tattctttga atagtaagaa tatggcacag ctgacagaag cagcaaataa attgaatcat
     8821 ttaacaccaa atgtcaaagc aatcgtggca gatgagatca aaatgtatat gatcaatgaa
     8881 gaagcctccg tggcagtgac attttctggc gaagctgccg atatgatgtg ggaaaatgaa
     8941 catttacatt atgtgatccc accagaagga tccaatcttt ggtttgataa tatcgttatg
     9001 ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca tcaactttat gctagaaccg
     9061 gaaaatgcgg cacaaaatgc agagtatatt ggttattcta caccaaatga agaagcaaaa
     9121 gcgttattgc caaaagacat ttcgtcggac gaacaattct atccatctga tgacacgatc
     9181 tcccatcttg aagtctacaa agatctagga tcgaaatacc taggcatcta taatgaccta
     9241 ttcttagaat tcaagatgta taggagataa tggcaaaact cagtaagaat ggggacttat
     9301 caacgttcta aagagctaaa aatcgtcctt tttctatctg agttttccaa tcttctacca
     9361 aaagcggatg aaacgccgct ttttttgtgt tattttattt tttctaccac gtttttctac
     9421 cagagaaaag tggacaaaac cattgtaaaa tagggcccga gacgggctag taaatctaag
     9481 attgaaaaag ttacagaagg ataaagtaat tggaaactat gcaaatatac ttaacccttc
     9541 tgtaatctat taataaaact aatatatgaa tccagttcaa tatttgtagc tatgaaaaaa
     9601 cataaaaatc atattagata aaaactaaat tggtagataa attattttgt cagatatctg
     9661 aggcaaatat tcgactattt gtgtttcaaa gtaaaaattg gaatacaaat atagaataga
     9721 catattgcag ccgatagttt attatgcttt tagacacatt aaactggagg aaaaaatgaa
     9781 aaagagcgct aaagatcgag tttttgatga gctgatacga ctaacaggga atatgtcttc
     9841 tgtcactgcc gagaaaattt cgaagaatct aggtatctct cgtcagaatg ccagccatta
     9901 tttgacgaga ttagtggaag ataaaaaggt cgagaaatta ccgggaaagc ctgttttgtg
     9961 gaagccttta gatgaatatg cggttgtaga taatacggag caaataaacg aagcctttca
    10021 ttctgtagtt ggtcatgacg gaagtctgag agaggtcatt caaaaatgta tagccgctgt
    10081 caaatatccg cctaacggac tcagtgtatt aataaatgga gccacgggtg ttggaaaaag
    10141 ttttttagcc acaaaaattt ttgaatacgc tatccatgag caaattattg aaaaagatgc
    10201 accatttgct atcttaaact gtgccgatta tgcggacaat cccgaactgc tttcggcaac
    10261 actgtttgga tacaagaagg gggcctttac gggggctgaa aaggataccg atggtttatt
    10321 ggcaacggcc aacaacggct atctttttct cgatgaggtc catcgcctat cgaaagaaaa
    10381 tcaagaaaag ctctttctat ttattgatac tggaaactat cgtccagtcg gtgaaaatgt
    10441 aaattggcat tctgctaagg ttcgttttat tttcgccacg acggaaaaag gtgaaaatta
    10501 tttattagac acctttgatc ggcgtattca aattagtgtt atgttgccaa cttttgaaga
    10561 tcgccccatt cgagaacgat tagagctgat tcaattattt tttcaaaatg aagcggatgt
    10621 tttacaaaaa gatattattg tttcaagaga agccttgtct ttaatgttat ctcatccctt
    10681 tagtgggaat attggtaagc tgaagaatat cattaaaatc agttgtgcgg atgtgtatag
    10741 tcgaacacaa gaagaaccat taacgattgg gaaaaatgag atcttgattc agttaaatat
    10801 gctggaatcc gaagtagaaa gcttgccgat tgctagttta ttgataaaga aaaatcaaaa
    10861 gataaaaacg gatcgaatca aaccgtttga ttatgattcg attttggaaa aaattactca
    10921 gctgataaat gaaggtaatt tcaatgaaaa tttttcttcg ataaagaatg aagtacagca
    10981 tttggtgcat cacattgata aggattcttt tttatcaatg agtagaaaat tgatgattca
    11041 gatgtttaga aaggtctggg tatccgtttt aggaaaaacg tacggattga cgacgagtgg
    11101 acctgttgca gaaattatta gcagatccta tgtcatttcc ccaagctata agacagactt
    11161 aaccgggata aatcgtacat tagctgcaaa tctacctcgg acgacttact tatgtaatca
    11221 gttcttggag catttaccac cgctctcaga aaaggacgag aactttctaa gactgcttat
    11281 tgtcgtgact ttatctgatt atgtagatga atccatcgag ctgaagggcc tgttgcttgc
    11341 tcacggagat agtacggcta gcagcattca agcagtagtg aatcaattat gcggcaatta
    11401 tgtttttgaa gcgttggata tgccagtaga gagtaatgtg tcagagattg tcgaatcgac
    11461 aaggaatttc gtgaaacggc aagtgaatac agatagtcta gtgctgatcg tagatatggg
    11521 ctctttaaat caaatttaca cacagatcaa gaacgattta aaaggggagt tacttcttct
    11581 gaataatttg acgacttcca ttgcacttga tattggcttg aagatgacga tgaatgcgcc
    11641 atttaaacag attgcggagg atgcagcaga caagtattca atcaatgttc aatactttga
    11701 aggcttttct caaagtagca acattatcat ttcctgtatg tcgggtttgg ggatttcgga
    11761 caaattgaaa gatgtttttc gtcatcattt atcggatgaa tccatcgaag ttttcacgaa
    11821 ggactacaga gaactacgtg aattaatcga ccaaaatgat gaaacttatt ttgacaagac
    11881 aaaattggtc atcacgactt cggatcttcc acgttccttt tcaattccta atattaatgt
    11941 ttatgacatc ttagacggtg atggagcagc caatttggag catattttga gtcctgaaat
    12001 gaagccggca gaatttaacc gcttgattca ggagctagta aaattctttt cgttggaagg
    12061 aattgcggac cgtttgaact ttctgaaccc aacgatcgtg atgagtgaag tggaaacagt
    12121 catttctaaa tatgaaaatt actatcattt cactttaaat ggccgtgtaa aactaaatct
    12181 ctatatgcat acggcattga tgatggaacg tttattcttg gctcggacag aaaaatctga
    12241 agaaccgaat tatgatttga aaccagatga agatgaattt tattatgttt ctagaagtat
    12301 ttttcaatca atggagatga aatataattt taaagtcaat gcttatgagc tttcgctgct
    12361 ctatgaatta ttaggaccgt atattcaaaa ataaagtgtc taaaaaagtg tctaatctgt
    12421 tttttttagg ttagacactt tttttaatgt gaattaacgt gattaaacca atgtttttaa
    12481 aagttggcac aggaattgct tatataaaag tgaacagaaa gaaaggaatt aaccatatga
    12541 agaaaattct aatagcgact cacggtcact tagcttcagg ctttcttagc agtattcaat
    12601 tactgacggg gaaggttcaa gagattacgg tgattaatgc ttatgtggat gagtgtgatt
    12661 ttgaacagga gttgaatgta tttgtaaacc aggtgaacga gcaggatcag atttttgttt
    12721 ttaccgattt atttggcgga agtgtcaatc agaagattac caaaaccttt ttggagaaag
    12781 agattgctgt aactattttg tctggcttta atctgccaat tctgctcgaa atagtattgg
    12841 caaccaagga attaacaaag gaggatattc aggaactggt aaataaatgc caacaggaat
    12901 taaagatgac cattcttgat gaggtacatg tagtaaatga agaggaagat tttttttagg
    12961 aggaagaaaa atgattaaag cagtgcgaat tgatgaacga ttaattcatg gacaagtagc
    13021 gatgacttgg acaaagagtc taaatttaac cgggttagta gtagcaagcg atgaggcggc
    13081 taccaacgat attcaaaaga tgacattgaa gatggctgca ccatcgaatg tcaaagtcat
    13141 tattaaaaca gttgatgacg cgattcattt gttaaaagat ccgcgggcga aggacatgcg
    13201 cttgatggtt ttagtcccta atgtcaaaga tgcggtaaaa gttgcgaaag ctttcccaac
    13261 ggaaatcgaa ttaatgaacg tggggaatgc tgggaaaatg acaccgccat cggaagataa
    13321 gaaaacctta tcaaaggaag tgatgttaac gacgtcagaa attcaagact taaaagaatt
    13381 agttgaactt tatccagata ccgtttttca gcctacaccg gcgatggaaa aacgttcagc
    13441 tatctctatt ttgagtgatt tttagaaaaa ggaaggagaa aaattatgta tgatgcattt
    13501 gtagtcgctt tggccgtttt tattggagtt gctggacatg aaatgttcgg gatgtccatg
    13561 ctgagtcgtc cgattgttgt tgctccgtta gcaggagcgt tgatggggga tttgcagacg
    13621 gggttgattg tcggggcgtc actagaagcc atctttatgg gtgtcgtcaa tatcggaatc
    13681 tctagtacgg cggagccttc tttggctgct gggctggcta cagcgttcgc aatcaaaatg
    13741 ggaggaaacg tggatgcgat tattcccatt gtctttccac tagcagtatt aggattacag
    13801 ttaatgaata tgattttttc ttttgtatgt ggaccgatgg ctgctaaatt tttcaaattt
    13861 gcaaaatctg gtgatcaaaa aggaatcatc aatctgcact tctttatgtg gtttgtccac
    13921 tatgcgcttt atgctcttat tccattcttt gccgttttat ttggagcaga tgttgtcaaa
    13981 agcgtcttgg ataatattcc agaggtcatc atgaacggat taacagtcgc tggaaatctg
    14041 ctgccggcag ttgggatggc gatgctgtta cggatgcttt ggagtaaaaa tattgctgtg
    14101 tggtttttct ttggggccgt tgttatggcg tatctcgagt taccattgat tgcaattgcc
    14161 gcagttggaa cgattattgc aattgttata gcccaaagag atctacaact aaaaaatata
    14221 tctgttcatg cagttcaaag cgatgctgct gtaaatgcaa cgcttgacca agaagaggag
    14281 gatttctttc aatgaaatta gctgatggaa tttctaaaga tgagaaaaaa ttggttcgga
    14341 agatgttttg gcgctcgatg actatgtatg cttcggtgaa tccggtgacg atgggcggcg
    14401 gtggtttcgc ctattctatg atgcccttca ttaatcattt ctacaaaagt gaagaagatc
    14461 gagctgcggc gctggaacgt catacacagt atttttcgac gaccattcct tgcgcgtcct
    14521 ttgtcatggg gattgcggca tcgatggaga aggaaaattc tgaaaaagct ggttttgacg
    14581 tggaaagtat caacgcgatc aaaacgagtt tgatgggacc attggctgga attggcgatt
    14641 cattgttctg gggagtttgg cgtgtcgtta ccgcgggact agcgatcaac ttagccaatg
    14701 ccggaaatat tttggcacca attatctttt tagttatgtt caatatacct aattacttga
    14761 tgcgatacta tggcggcgtt ttaggctatt cattaggctc taaatatatt gaaaagctgt
    14821 atgcgaacgg gttaattgaa attctgacaa aagctgctag tatcttgggt ctgatcatgg
    14881 taggtgcaat gacgatcagt atggtcaaat tcaataccgt tttgagcttt tcaatgaagg
    14941 gcgaagaact aatgaatgtc caagaaatcc tcgatagtat ttttaaagga attgtaccgt
    15001 tattggtaac attgagctgt ttcaaattac tagataaaaa agtcggcgtc atcaaaatta
    15061 tttttggaat ggtcgtcttg ggaattttat tgtcattact tggaatcgca tagtaggaag
    15121 ctgcagctac taggctgcag tttttttagc aaggagggaa aagatgacta ttaatgaagt
    15181 aatttattca cccaaacacc cattttttaa tgctgagtta aacgacggaa atgtgactac
    15241 ttcaaacgaa tacagctact atcggaatat tcctgatgta aagggattga ccttcgatgt
    15301 aagtaatcaa aagacattag caaaccttga tgtttgcggg acgattcaaa cgttgacctt
    15361 ttatcgagaa aatttactaa ctgaggaaaa accaggtgtt tgggtcaata aacagctctc
    15421 tcaaaccaat tcgttatcct taaaggttga agtaaacggg attgtctacc atttgtcaga
    15481 atcggatcat cgtattgaag tcgatgtgat tcaagactgt ttgcctagat atattcatca
    15541 ttattcgacg tttaaggtca ttgtagtatc ctttgctccc atactggcta ataagcgttt
    15601 aagtatgctt gttcaacagg tttacattgt gaatgaaaca cacgaaagcc ttcaagtttc
    15661 agtgaaagat gtgccttcgt atcaagagaa gtattcggat cagcaaaatg tgttgatttc
    15721 tcaaaaagga aataaggaca cgatcgcaca acaggaagtg gcaagcttcg ccattgcttt
    15781 tgtggaccca aatgcttttg aagaagtaat gacttttcag gaatctgata tcgaaaaatg
    15841 gctgaaggaa acattgaact attttgctca gctatttggg cagctgtcaa tgccaaacga
    15901 tagagttgtc catctctatc gtcgtgcact ttatcaaagc ttttctagtt tcggtatgaa
    15961 tcgacaggga caggtcgtcg gcagcaattg gggcagctat cctgctacta atcgaatttg
    16021 gaataaggat atgtattacg catcattgcc attcaccatg tttgaaccgg agttatgtca
    16081 aaaaacggtt ctatggtttg atcaatatgg cgttaagttt cctggttcaa aatttacagg
    16141 aggtatcaat cattctctga gtaattcgtt atccagtgcg ttactggcta gtctttattt
    16201 tgaacatact ggggatacaa aattctttga gcggtgtccg caggttctac tgaatgcttc
    16261 aagaattatt gaggacatat tagctcaacg tcggtcaggg gaaccaatgc tttttccatc
    16321 tgtttggtta tcagacgcct ttgcacttgg aaagtatcat accggcagca atctttgtct
    16381 ttggaaggcc tgtattggac ttgcagaaat ttttgaagcc ctagaacaac tgagtcttag
    16441 caagcgatac aaaaacattg cctgtaaact aaaggaatca atttgtaaac aaatgactat
    16501 cgatggtcct tttggtgagc aatttttgga gggaatcggt gatgaggaga aaacaaccta
    16561 ttcagtgaag cactatcaaa aaccgattct tgagcaaggg ttaatctttt taagtgatgt
    16621 gattgtggat ggaaaaatta atttattgat gcatgatgga gaagaatcag acactacgtt
    16681 gatgcctttt tatcaatttt tagataataa agatcattta tatcagaaca ccatggcctt
    16741 ttctgctagt tctttcaatc caacctatag cgaggaaatc aaaggcatca cttggggatt
    16801 agaatccggc gccacttttc ctggctttat cactgtttta atgagcgagc tgcacaaccc
    16861 tcaagctttc gtaacccgat tagaagaact aatttgtttg gcagatttag acggttcgtg
    16921 gtggtggtgg ccgtatcgct tagaggctca acaaggagaa gtcgtgcgag actttggctg
    16981 cggcaaatgc ggctgggcat cgggtctttt tgtcagccta atgatcagtc aatatttcgg
    17041 attgaaattc aaaaaaggag tattgcaaat tgatcctgtt gataatttaa cgttttcttg
    17101 gaagaatttg aagttcggtc aagccttcat ttcgattgaa tgtacgcaat ccgagttgag
    17161 tatttcaaat ttgtcagaaa agccattgaa aataagtgat acgaaaaaaa ttcttcacgt
    17221 agaaaaggga aaaacgattc acattcaaag gagaaaaaga tgagtatact aaaaaatatt
    17281 acccaattta aaaaaatcca gagtcgagca atcactcccg aaaatccaac aggcgagaaa
    17341 ggtaaagctg cgatgttgcc aagcgagcta ggaccaagcc gcaaaggtcg tggcgcaatt
    17401 ccgctgactt ctggtgaaga aactgtgatt gcggatatta ctggaacggg aataattcgt
    17461 catatgtgga tgacgattcg tgatcgaaca gaatttggca gttttgtttt acgtgattta
    17521 atcttaagaa tttattggga tgggtcggat agccctgctg ttgaaagtcc tttaggggat
    17581 ttcttttgta atgggtttgg agagcgttgc gaggtgaact cgttgccgat agtggttaat
    17641 cctacagggg gctttaattc ctatttcgaa atgcctttcc aaacatccgc taaaattacg
    17701 atcgagaatt tacatccgaa ggatatgtcc tcattttttt atacgataaa ttacgctttg
    17761 gtggacgaat tggaagaaaa tagcctatat tttcacgcaa attggcatcg ggaacgcatt
    17821 accaaattac aggaggatta taagttaatt gatcgattaa agggtcaagg ctattacgtg
    17881 ggtacttacc ttgcgcttac ctgcctagaa cgttattggt ggggcgaagg cgagtttaaa
    17941 ttctacattg atggggatag tgagtatccg accgtaacct caacaggttc agaagattat
    18001 tttggcgggg cgtgggcctt tcatgagtac gatgagttcg gacgtccaca tgcgaaaaac
    18061 tattcaacag cctttttagg gtatccttac caatcaaata gagatgccac aagagatcgt
    18121 tttgaaacgg gaaaattgaa tgccgttcat gcctttggtg acgacgcaat gccacgacat
    18181 ggtctttatc gttggcatct tcaagacccc atctgtttta ccgaagattt atcggtcact
    18241 ttacaacaaa tcggcaacga tgatgttcgc ttatttgagc ggcaagacga tgttgctagt
    18301 gtcgcttact ggtattcaac agaagcttca ggaaatacta aacgtcattt cactgatcga
    18361 gaacgcttgc cgcgttaatg aagtgttttt cacatattcg aagggaggaa cctatgatga
    18421 tatatggggc aattgaagct ggagggacta aatttgtctg tgcagtcggt actgaggaat
    18481 taacgatttt ggaaagagtc agttttccca caacaactcc agttgaaaca atgaagcagg
    18541 tcattgattt ttttaaacct tttaaggaac aattggcagg catcggggtc ggctgctttg
    18601 gtccaattga tattcaatgc aaatcaacga gtttcggtca tatcacatca acaccaaaac
    18661 ttgcttggca aaatttcaat tttgtaggaa cgctaaagca atatttcaat attccgattg
    18721 aatggacaac agatgtcaat gcggcttgct atggtgaata tgttgctggt tctggcaaag
    18781 ggttagcagg tgttgcatac ttcactgtgg gaacaggtat cggaggtggt gccttgttca
    18841 atgggacttt cgtggaaggg ttcagtcatc cggaaatggg gcatatgttg atcaagcgac
    18901 atccaaagga caatttttca ggaaactgtc ccttccacca agattgttta gaggggatgg
    18961 cagcaggacc agcgattgaa aaacggttgg gtgtaaaagg ccagaattta ttggctgacg
    19021 attcgttttg gcagattgaa gccttttacc ttgcccaatg tgcatacaat acaacactga
    19081 tgttttcgcc agatagaatt atttttggcg gtggtgtaat gaaacaagaa cacatgaaga
    19141 aaaaagttca ggataaattt gttgagctta taaatggata tgttgaaata ccaccaattg
    19201 atagctacat tattacgccc gaattaggtg ataatgccgg aataatcggc ggattagcat
    19261 tggccagaaa agcggtaaga aataagcaac cataattaat gaatgtttca ttgatactca
    19321 tgtcaaaagt atttaaccga gtaatcaaat gatcaaatga agttggataa agcgaaaatg
    19381 aattcaaata aaaaggctct aagtcaaatc gtgttgtttt cattaaatca ctgaaaggaa
    19441 atgcttctgc gtttcctttt ttctttttgt tgtgcgctgt ataccttgag gatattcact
    19501 ggtggcgtac acttaaatag taatatattg tgttacttta tgtctgtggt aatagttata
    19561 gttgcttacc aatgtatgtt catttttcga aatgttgact tcataatcct tggcttaatg
    19621 tttgaatttt tttggtgata gattttttca gccgtataaa ataaatcgta attctattat
    19681 agagagttgt gattataaaa agatagcaag aaaaaaaaag aaagtttcta tacattttaa
    19741 atattttatt cctatttcta ttgctaaaaa agtagaaatt ctatatttgc ctttaactct
    19801 cgcttctctt gcaaatgtag ttagcatttt aatcgataat aaactcaggt agttatatta
    19861 ccttgaaatg attgataatt ttgtcacttc tatttttttt gcaataaatt ttttatttta
    19921 gtgaaaaata agaaactatt tctttgaagg cgataagtta gaaaaatatt attgtagtct
    19981 tttctttatt aggaatcatc actgttgtat cttattacct tttctgtttt tcttattaga
    20041 gaaaaataat ttgagaggta gatagttttt attggaggaa gtaattatgt atatttttga
    20101 aacaaaaatt tttcattgca ataattgcaa caaagaaatt tcatcggggg aagaggtttg
    20161 gacaaaaatg gaaatttccg ccaaaagtaa ctgctcctca actgaaatca attaaagaac
    20221 tagaatttga gaatgcactg gtaatttgta tgacatgtac tgatgaagta tttcgaaaac
    20281 atttttaaag gttgagatgt atatagattt aaccaatcat ttatctctct cggaaaagga
    20341 atattaacta accagcgggt tatcaaaaat aggtaggaaa actgtactta atggcttatt
    20401 tcgcgctgat tatgagaata tcagtctgtt tgattaattt ttagggttca aaatacagag
    20461 acaaactcca ggtcgggttt cgccacaagc gaatta
//
LOCUS       DACECR010000047        20080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.047, whole genome shotgun sequence.
ACCESSION   DACECR010000047 DACECR010000000
VERSION     DACECR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.047"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            156..935
                     /locus_tag="IWO17_001598"
     CDS             156..935
                     /locus_tag="IWO17_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290481.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            981..1925
                     /locus_tag="IWO17_001599"
     CDS             981..1925
                     /locus_tag="IWO17_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0290482.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1941..2720
                     /locus_tag="IWO17_001600"
     CDS             1941..2720
                     /locus_tag="IWO17_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ0290483.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            2732..3430
                     /locus_tag="IWO17_001601"
     CDS             2732..3430
                     /locus_tag="IWO17_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ0290484.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQVLAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3458..4222
                     /locus_tag="IWO17_001602"
     CDS             3458..4222
                     /locus_tag="IWO17_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ0290485.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            4235..6052
                     /locus_tag="IWO17_001603"
     CDS             4235..6052
                     /locus_tag="IWO17_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0290486.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAVFGSLSASAVSSIIILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYVETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAARVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            6063..6695
                     /locus_tag="IWO17_001604"
     CDS             6063..6695
                     /locus_tag="IWO17_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0290487.1"
                     /translation="MKAIIVGAGGFGKEIAFLLQSVSRYELVGFVDDSLKMQNQELLE
                     KPILGTIDLLIELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVAPSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSVFPSVNISGHVILGD
                     KNEIGVGTKIIQNLSIGNTNIIGAGSVVIKNINNNTKNVGVPTKVIERWD"
     gene            6697..7815
                     /locus_tag="IWO17_001605"
     CDS             6697..7815
                     /locus_tag="IWO17_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ0290488.1"
                     /translation="MENKRILLASPHMSEEGYEQEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNEKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHNFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINTNHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            7808..8425
                     /locus_tag="IWO17_001606"
     CDS             7808..8425
                     /locus_tag="IWO17_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0290489.1"
                     /translation="MRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLGS
                     PIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKILRATSLDELPE
                     LWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKFD
                     LDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
     gene            8433..9647
                     /locus_tag="IWO17_001607"
     CDS             8433..9647
                     /locus_tag="IWO17_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0290490.1"
                     /translation="MPLNILFLTLLDFQSLEESNIYTDLMSEFVKNNHNVSIISPTER
                     KKRIKTHLIEINDKCEILKLRIGNIQKTNLIEKGFSTIQIERKFISGINQYFSNKKFD
                     LVIYSTPPVTFAKVVKFIKKRDNCKSYLLLKDIFPQNAVDLNQLSKNTYIYKYFRKKE
                     VSLYEVSDYIGTMSEQNSQYIRKHNKIDEQILEVNPNSIRINENKIGSPLDKEEIRNL
                     YKIPKEKTIMLYGGNLGKPQGIDFLLNIISSLEKDNKILLLIIGSGTEYGRIRKHINT
                     NNIKNTILIDSLSKNEYKKVESIADIGLVFLDKKFTIPNIPSRILGYMESKKPILAAV
                     DKHTDLNEMIKTGKFGYCSEHGDLNAFLSNMERLKDEKIRNELGKNAFIYLKNNFDVK
                     RSYSIIMNHFEL"
     gene            9663..10685
                     /locus_tag="IWO17_001608"
     CDS             9663..10685
                     /locus_tag="IWO17_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0290491.1"
                     /translation="MFKDKILLITGGTGSFGNAVMKRFLDSDIKEIRIFSRDEKKQDD
                     MRKKYNNDKLKFYIGDVRDINSVKNAMYGVDYVFHAAALKQVPSCEFFPMEAVKTNIM
                     GTDNVVTAAIEMGIQKVICLSTDKAAYPINAMGISKAMMEKVFVAKARTVTDERTMIC
                     GTRYGNVMASRGSVIPLFIDQIKNGKPLTVTDPGMTRFLMSLEEAVELVVFAFQNAKA
                     GDIMVQKSPASTVGDLAQAIKELFHADNEIHIIGTRHGEKRYETLLTKEEYLVAQDMG
                     DFYRVPADTRDLNYDKYFVEGSKELTQEGEYNSDNTQRLSVEQIKERLLELDYVRKEL
                     KEWQNQ"
     gene            10682..11791
                     /locus_tag="IWO17_001609"
     CDS             10682..11791
                     /locus_tag="IWO17_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HAQ0290492.1"
                     /translation="MNILVTGSNGFIGKNLVAELKNEGFTDILSFDRDTPVHLLDEYC
                     EKADFVFHLAGVNRPKDDAEFMTGNFGFTSELLEKLKEHGNTCPVMISSSIQAELNNS
                     YGKSKKAGEDLLKKYQKETGAPVYIYRFSNLYGKWSQPNYNTVVATFCYKIARDEEVT
                     VNDPSAIIELCYIDDVVKELLHCLNGQPYTENGYCAVRETDEVTVGELKELITSFKNS
                     RTNLSVPNLARRVEKNLYSTYLSFLPEDEFSYFLKMNVDNRGSFTEFLRTKDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGEGVIRFRKVDSNEIIEYFVSGEKLEVVDIPVG
                     YTHNIENLGSTDMVTVMWVNEPFDPEHPDTYYVEV"
     gene            11795..12919
                     /gene="wecB"
                     /locus_tag="IWO17_001610"
     CDS             11795..12919
                     /gene="wecB"
                     /locus_tag="IWO17_001610"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAQ0290493.1"
                     /translation="MKKLKVMTVVGTRPEIIRLSAVINRLDRSEAIDHILVHTGQNYD
                     YELNEVFFEDFKLKKPDYFLNAATGTAIETVGNILIKIDPILEEVKPDAFLVLGDTNS
                     CLCAIAAKRRHIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINLTYSDIAREYLLRE
                     GLPADRVIKTGSPMFEVLNSRKDDIQHSDVLNRLGLKEGQYFVVSAHREENISSETNF
                     LHLVDSLNTIAETYQLPIIISTHPRTMKMIEAKEIKFHELIQTMKPMGFNDYNKLQIN
                     AKAVLSDSGTISEESSILNFKALNIRQAHERPEAMEEASVMMVGLEKERIMQGLEVLE
                     TQQKDTLRHVADYSMPNVSEKVLRIILSYTDYVNRVVWGR"
     gene            12916..13971
                     /locus_tag="IWO17_001611"
     CDS             12916..13971
                     /locus_tag="IWO17_001611"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0290494.1"
                     /translation="MRIVVNDIAASEGGAMSVLRDLHSDIISSGDENEWIFLLGEKFI
                     EESDNIKVLTFPKIKTSWIKRLDFDFISGKKIINKLQPDIYLSLQNTATIGVKAPQYV
                     FLHQPLPYQKEKNFSFIKKKEFKMATYQHVIGKIYSILFKKTKATIIVQTQWMKENVS
                     SILNNKILKVDTKINIDENIINKQINENKRNMKHFFYPASNIPYKNHDFLIESFSKCK
                     DKELRLFLTLSRDEIDIKDDRIILLGKVPREKVFDIYSKSNLIFPSSIETLGLPLLEA
                     QCFMSPILALETEFSKEALSGYPHKYLFKLDNLDNLSHLIEKISTSSIVFNVENVIYK
                     KENRVSILDILKGENIY"
     gene            13968..15182
                     /locus_tag="IWO17_001612"
     CDS             13968..15182
                     /locus_tag="IWO17_001612"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0290495.1"
                     /translation="MKKFVVAHPAQQHSYKIAEALIEKEMLFKYITSVYLKKGSILNK
                     LLNIIPGENKLRAKGRHSDKICDEEVLLINEFSGLLLLILQRVKFLKHLYKKYWDFHN
                     KKFNKKLIKYINSNIDEISGVIVFDQVSFDFFKKKINIPIILDMSAPSVVFMKSQFEK
                     YGISTLKYDALLKKNNFEINSADFFIGASSITERSLLNMGIEKKQIKIFPYGVNIVKE
                     NDKLMNLRTRKKRLKFLFVGRVTLEKGIGVLDSFFEKFGIEDAWTFAGDYSDSKKFYE
                     KYEDKVQFVGHVSKIKMFELYRDNDVLVFPSLADGFGLAVIEALSNGMYVICSDSTGA
                     KDIIMEGKNGDVFLTGDENDLNNKIQYYKKNINKIDRLKIKETVNDLTWERYNKNIIK
                     SLEYFTVGMNNE"
     gene            15175..16290
                     /locus_tag="IWO17_001613"
     CDS             15175..16290
                     /locus_tag="IWO17_001613"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290496.1"
                     /translation="MNKLLMMLNFFYLYVYMIDAYGIINNDLYLRMVLIILFIMIGLI
                     NCIKDIMNKIYSKDNLFFTLYLIYFLSTQYMLWNIDISQLIMIIYTPMMFIAFSRFHI
                     DRYFYEKFTFSYILLMTFTYFYTRFFLNINNGLVINSCYYLLLTVPIVYLFVNPYRKN
                     ISFLLISIIILFSMKRTALITLALIIFIELILNVLNKKRISLRRLAFLIPFSLFIAAL
                     LIKINKIISSLYDNNIFMRFYSGDTIGESRGDILKLLLEKICTNSNIYFILLGQGINS
                     TIYYTGGLTAHNDFLEVLFDFGIFGLLLYIMIYIIFIKKIRIITNVKDKRSLLYTMVI
                     LMLVSVSSHLIFIPSYVSLLIMNYNYLKNKNYFSKEN"
     gene            16290..17384
                     /locus_tag="IWO17_001614"
     CDS             16290..17384
                     /locus_tag="IWO17_001614"
                     /inference="COORDINATES: protein motif:HMM:NF016145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HAQ0290497.1"
                     /translation="MKKIGILTFHRAENYGAVLQTYALQEYISKMSSNYEVEVIDYRS
                     PFIEKYYKLLSFKAFTNIKSLKRIVYVLLYNGTLKSKRKIFDNFIDKFIRVSLSEYDD
                     KTIRGLDDKYDLFICGSDQIWSPYSAGFDPIYFLDFVKKRNKKNAYAASFGVEIVPEE
                     TIKEYSRRLKNFNVISVREEIGKKIIKNLIEEDVPVSLDPTLLLSKTEWDLIIEKKRK
                     ISEKYLLIYLITETREIIELAKKVAREKKLKLVYINDNIYKTKGIYNYNNCSPSDWLN
                     LFYNADYIVTNSFHGIAFSINFEKEFSVQYLPEPAKVNSRINNILDNYSLKWRINYDG
                     NEKINYSKVNKILVEDRVASQQILKDIMEY"
     gene            17394..18377
                     /locus_tag="IWO17_001615"
     CDS             17394..18377
                     /locus_tag="IWO17_001615"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0290498.1"
                     /translation="MKPKVSIIIPAYNAEYTISRALESALKQTYSNKEIIVIDDGSKD
                     RTLAICKEYQLNNHEIFIFSQKNSGVSVARNLGIRKADGEFIQFLDADDAMEPNMTEK
                     LIEVQRNNDSDYVICGLTIIKDNNIVRTPHLKERNINFINFEDYKYIYPLLAAPCNKL
                     FKKELIVLFEKNFSNGEDLLFNTEYLTKIKKISIIEDCLYKVYLDNENSLNKKMSIST
                     VKSLLLINEYEKEIMNKLFDNDNVLEFNVFNKFKIIQTSLQKIVNNEEKDDVIELINN
                     LRSDSQFLTILEKTNMKNMKSNLIKFCFSYCDSQTIYQLFFLISKVRGYFQ"
     gene            18374..19810
                     /locus_tag="IWO17_001616"
     CDS             18374..19810
                     /locus_tag="IWO17_001616"
                     /inference="COORDINATES: protein motif:HMM:NF014051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0290499.1"
                     /translation="MKDRVKNVIYNAVYQVFIIVIPILTMPYVSRKLGAENLGINTYT
                     QSLVLLLGLFINSSILQIGAREIARQDRISRTKTFITLWLLQVLCGLVIIIVYILIIM
                     GSKYYYYYLLQLPFLISYIFDISWFFVGLEKIKNVVFRNMFVKVCTIFLIFLLIKNKN
                     DLSIYIIIISLGSLVGNFIFFFSLKKYLDFDKIVGLDLSNLRPFINSIVILLVPQVAI
                     QLYQYIIKFSIENSTTTTELSYFDQSQKIARIIIQLVSSISIVLMPVIANLDKNKSRN
                     ALNKIIQLSVNYSLSIAVIFTILLVIISPTFVPIFFGPEFKEMIPHMIISSFIIIPIC
                     VGGVFSNQLILGMGLYKYYATPYLIGGSIGILICLIYKDKMSGLIGCIVLVIVESLVC
                     FFRIIFANKIIDIKENLQGTIKEFFLLFLLLAEVYIVNTIFTPSPSALLNLFYMMIII
                     IQYFVLSTIIKTETAKDLSKRLKSFLIK"
BASE COUNT         7486 a         2844 c         3657 g         6093 t
ORIGIN      
        1 aacagtttgt ggtatgatta tatagggaaa acttgtttac aaatatttct gtgaatatat
       61 tagtttaatt atgtttttta aaataaaaag ctgtcatttt tatgtaagtg aattatatct
      121 taatttatct atcatcaata cttaggagtg aatctatgga aaagccattc gtcaagtcat
      181 tgactgctgt ggcggtaggt gcaggtgcta ttgcgatctg tttgtttggt taccatatca
      241 ataatcagcg acaacatcag caacggatca attatgcaga atctgccatc accaatcaaa
      301 aagacacctt gacttcttta tcaaaagaag tagacaagct gtatagtaca aaagaaaaaa
      361 tttttctaaa ccctgaaata acagaagaaa caatcacaag tttgtcaaat aagctatcct
      421 ctgtcaaact ttcagcagat gattacgaca tcaaagaatc agaattgcct aaagaagctg
      481 ttgcaatcca agatgagaag aaagcaatcg tgacacagtt agatagtgca gaatcaaaac
      541 tgaagatcca gacagctgtc aacgatttat ttactaaaaa tgtatcaaac tggcagcaag
      601 ctgtagacga tgtcatcatc aaagaaaagg tgacaagtgc agatgtcgct cgtgtacgtg
      661 aaaatatgag cttttttaaa gatagtcctt ggaaaacggt tgttatgcaa tatctaggat
      721 tcgctgatac tcagatcgcg caagtcacgc aactagatca gttattcgat acgatgttga
      781 aggatggtca agtgactgcc accgctactt acgatcaata tttgacggtg ctcagtcaaa
      841 tagagcagat ccggaatgaa aaaatacgcg cagcatacgc aacaaaagca caaactgttg
      901 ctcagcaaat gggctatagc aatataagct attgacagtt aaaacgttga ttcaagcaag
      961 gttagcaaac ggaggaaact atggcaagac gaagaagaaa atcaagaggg aaaaaaatat
     1021 ttctgattat tttagcagtt cttatcgtat tagtattagc aattgtagga gtaggagcaa
     1081 aattatatct ggatctctca cattctattg agaaaactta cgaatcagtg gaacgaaccc
     1141 aaggagaacg aacgaaacca gttgatctgg acaaacaaga atctttctct gttctattga
     1201 tggggattga tactggagat ttaggtcggg tcgatcaagg aagatcagat acgatgatgg
     1261 ttgccacagt cagtcctgaa gacaaacaga caacgatcgt cagcattcct cgagatacgt
     1321 atgtcgacat cgtaggtcgt gacacaaaag acaagatcaa ccacgcttac gcattcggcg
     1381 gagctgccat ggcgatggat accgtacaaa aatatgtaga catcccaatc gatcactatg
     1441 tctctatcaa catgaaaggg ctaaaagaac ttgttgatgc agttggtggt atcgaagtca
     1501 ataatgacct cactttctca caagatggct atgactttac aatcggtaaa attactttga
     1561 acggggatca ggcattatct tattctcgta tgcgttatga agatccaaac ggtgactatg
     1621 gtagacaaga gcgtcaaaga aagatcgtag aaggcgtagc aaaaaaagta ttgagtctaa
     1681 acggtgtcag caactatcaa tccgttttga atgctctaga gtcgaatatg aaaacagaca
     1741 tgagctttga tgaaatgaaa aaaattgctt tagattatcg ggaggcattt ggtacgatca
     1801 agcaagatca gctgcaagga gaaggcttca tgcaagataa tatctcctat caaaaagtca
     1861 gtgatcaaga acttgatcgt gtaaaaaaag aattaaaaga tcaaatgaat ctagagaata
     1921 aataggaaag tggagaaaaa atggaagaga caatcagctt gaaagaaatt tttgaaacat
     1981 tacgcaaaca cttggtaaca atcatcatct caatgtttgc tggcttggcc atctcaggaa
     2041 tcgtgacgtt tttcatcttg acaccaaaat acagttcaca agcacaattg atcgtgacac
     2101 tgccacaatc agacacagca aatgtcaatg atgtcaatac gaacctgcaa atgatcaata
     2161 cgtataaaga tatgatcgtc agtgatttag tactaaatga agtcaaagat cgtttagaaa
     2221 cagaagacaa tgtgaaaatg actgctggac aaatcaaaga tgcgatctcc gtcaatcagt
     2281 cacaaaattc acaaatgttc tcgatccagg caataagcac gaatgcagtg actgctcaac
     2341 agatcgctaa cacaacttca caagttttcc aagaaaacgc caaagacgtg atgaatgtcg
     2401 ataaaatctc gattatttca ggtgcagttg ccaatacgac acctgtttcg ccaaataata
     2461 aattgaatct agtgattggc ttagtgttag gaatgatggt aggcgtaggt ttggcattct
     2521 tattcgaatt acttgatcgg acagtgaaag atgaaaaata tgtgacggat acattaggat
     2581 tcccaatctt aggaacagtt ccagaaatgt cagcgaaaga attgaacgct ggtatccgta
     2641 aaaaaggaac agctcctgca cgaccaaaag ctacaaacga taaaacagcc ccatcacgca
     2701 gaagccgctc aagagtctag gaggaagaaa aatggcacga acacagaaac aaaaagcagc
     2761 acaaactcat gcagtcagtt tgatcacatt aacaaatcca tcttcaccaa tcgccgaaca
     2821 atatcgtacg atccggacga atatccagtt tgcctccgca gctggtcagc aaatcaaaac
     2881 gattgtcgtg acttcatcag gtcctggaga agggaaatcc accactgcag caaatattgc
     2941 agtcgttttt gctaaatcag gtcaacgagt tctactagtc gatgcagatt tacgtaaacc
     3001 cgtcgtctat aagacattcc agctgaacaa tgccagtgga ttaagtacag cacttagttc
     3061 atcaggaagt gtagcagatg tgatccaacg aacgccagtc gagaacttgt caattttgcc
     3121 tagtggacct aagccaccca acccatcaga attgttaagc tcgccaagaa tggatcaggt
     3181 tctagccgaa gcaagacagt tatttgacgt ggtgatattc gatatgccac cagtagtagc
     3241 cgtaacagat gcacagatca tgtcttcaaa aacggatggc actttattgg tcgtacgtga
     3301 aaatatgtca cgtaaagaat ccttgactaa agcaaaagaa ctgctggaga tggtacaagc
     3361 acgagtctta ggtgttgtgt acaacggagc agaacatagc aaagatgctg gttattacta
     3421 cggaaattaa ggaataagaa gagaggagac aagagaaatg atcgatctac attgccatat
     3481 cctaccagga atcgatgacg gggcagaaaa cttagaagca agtattgcaa tggctgaaaa
     3541 agcgattcag caaggcatca cgcacatttt atgcacacca caccacaata atggaaaata
     3601 cagcaatgaa aaatcacaag tcatttctct agtcgcttcg ttacaagccg aattagagaa
     3661 aagacagttg cctttgacgt tattggaagg acaagaagta cgtattaccg gaacgctcat
     3721 cgaagacatc caccgagatg aaattttatt tacagattta gatgatacat atcttctgat
     3781 cgaatttccg acactggaag tgccgttata tgcagaacgg cttttttttg agttgttaga
     3841 actaggaaag acaccagtga tcgtccatcc agaacgtaac gcgcattttc gcgaagaccc
     3901 aaatcatttg cttccgttcc tagatatggg atgtttggcc caactcactg cgccaagtta
     3961 tgttggggta tttggcaaag acatccagaa aacagccaaa acgatggtca aacataatct
     4021 tgtgcagatg gctgcttctg atgcccatgg ggtctctaag cgcacctttt acttaaaaga
     4081 gtgctatgaa cagattgcca aggactttgg taaggaaaaa gtagtacaga tgaaacaagt
     4141 cgcaaaggat ctgatcaacg gggatgaaat cagttatcca atctatgaag aggtaaaaaa
     4201 gaagaagttt ggtttatttt agggggaatg tagggtgttt gtattaacaa gaagaagaaa
     4261 aattgccaca ttggtatttg tggacagctt gttgttgatt gctgcgaata ttgcagcagc
     4321 taaattcatg aagccgtttg tagctatacc aatggacctt attttaatct ctatcggttt
     4381 gtcgattggt ttttatttat tctatggtag tttattcaaa gtattcactc ggatcaatcg
     4441 ctatacaaat ttaagagaaa tcattgcggt ttttggctca ctatctgcaa gtgctgtctc
     4501 aagtatcatc atattgctgt tcataaatcg gcgatacagt ttacgtttag tcatttttgc
     4561 ttacctgctt tcgctattat tgattattgg tagccgattg atctggcgta tctatgtcga
     4621 aacaaaaaat atgagatatc tatccacaga ttctgctaaa aatacattga ttgtcggtgc
     4681 tggcgaaggc gggcgtattt tatacaacag ctttcttgga tccaaaacgg ctcaagatat
     4741 ccacgttgtc gggtttgtag atgatgatcc aaataaaaga aatacgtatc tttcaggaaa
     4801 aaaagtatta ggtgcattaa aagatattcc ggaattgatt gaaaaatatg acattcaaat
     4861 ggtaacgatc gctatccctt ccttatcaag aaaaaaatta cgaagaatct tcgagttagt
     4921 cgaatccgct cgtgtgaaag tcaacacgat gccttctata gaagaacttg cttcaggaaa
     4981 aattagcgta tcaaaattaa aaacaattga cgtagtggat ttactgggaa gagatgaggt
     5041 aaagctggat attgaatcca tcaaagatca gatcacagac aaagtcattc ttgtcaccgg
     5101 cgcaggagga tcgatcggtt cagaaatctg tcggcaaatc attcaattca atcctgcaaa
     5161 gctattgctt ttaggacatg gggagaactc gatctacttg attgaccgtg agttacgcac
     5221 gcatcatcaa aattgtccta cagaaatcgt accgatcatc gcagatatcc aagatcgcga
     5281 gaaaatcaat gagatcatgg aacaatatca tccagatatc gtctaccatg cagctgctca
     5341 taaacatgta ccactgatgg aatataatcc aaaagaagca gtcaaaaaca atattttcgg
     5401 tacaaaaaat gtagcagaag cagccaaagc agcaagagtg aaaaacttcg tcatggtatc
     5461 gacagataaa gctaacaatc ctccaaacgt catgggttca acaaaacgaa tcgctgaaat
     5521 gatcgtgact ggattgaatg aagaaggctg tacgaaattc tcagctgtca gatttgggaa
     5581 tgtccttggt tctcgcggta gtgtgattcc agtattcaga gaacaaattg ctcaaggtgg
     5641 tccaatcact gtaacagact ttcgtatgac ccgatacttc atgacgattc ctgaagcgag
     5701 tcgtttagtt attcaatctg gcgcattagc aaaaggtggc gaaattttta ttctagatat
     5761 gagtgaacca gtgaagattg tagacctagc caaaaacatg atccgattga gcgggtatag
     5821 cgaagacgaa atcgaaatca ttgaaacagg tatccgtcca ggggaaaaac tatatgaaga
     5881 actattgttg gataaggaaa gaaatgatga agcagtttat gagaaaatct ttgtaggaaa
     5941 catcaaaggt tactcaatac aaaaagtgat ggattttgtc aagagcctgc cgcaagacga
     6001 tgaacaactg gctaaagaca ttgtcacttt tgcaaatgct tcaaataagt agggggagta
     6061 aagtgaaagc aattattgta ggagctggtg gatttggcaa agagattgct tttctattac
     6121 aaagtgtctc tagatatgaa ttggttggtt ttgtcgatga ttcgttaaaa atgcaaaatc
     6181 aagaattatt ggaaaaacct atattaggaa caatagactt gttaatagaa ttagaagaag
     6241 aaactgctat ttttctggga attgcttcgc cagatatcaa agagaaaata tatcagaaga
     6301 taaataaaaa taataaattg attttcccta atttagttgc accaagtgca ctagtgggca
     6361 taaatgttca gttaggaatt ggcaatattt tgatgcccta tacgacttac actgctgacg
     6421 tagttttagg aaatttcaat atgatcaata tcggctctac tatcggacat gatactcaaa
     6481 tcggtaatta taattcagtt tttccaagtg taaatatttc aggacatgtt atattaggag
     6541 ataaaaatga aattggagta ggtactaaaa taatacaaaa tctaagtatt ggaaatacga
     6601 atattatagg tgcaggaagt gtagtaataa aaaatattaa taacaatact aaaaatgttg
     6661 gcgtacctac caaagtaata gaaagatggg attaagttgg aaaataaacg aatattatta
     6721 gcatctcctc atatgagtga ggaaggatat gaacaagagt atgtaaaaga agcatttgat
     6781 acaaattggg tggctccttt aggtgcgaat gtgaatggat ttgagaaaga attagcagaa
     6841 tacgtgggag ctagtcatgc tgccgcatta tcttctggaa cagcagctat acatttagct
     6901 ttgaaagcgg ctggggtcgg taaagatgac atagtgattt gtcagtcctt gacgttctca
     6961 gctactgcta atccaattct ctatcaggga gctatacctg tatttgttga tagtgattac
     7021 gaaacttgga acatgaaacc tgaacatttg cgaacagcgc tagaaaaata tcctaacaca
     7081 aaggctgtat tagtagtaca tttgtacggt ttatcagcta acttagacga aattactaaa
     7141 atatgcaaag aatatgatgt tactctaatt gaagatgctg cagaatcatt aggaacaaca
     7201 tataaaggaa aatatactgg tacattcggc gactatggaa tttattcttt taatggaaat
     7261 aagataatca caacttcagg tggcgggatg cttgtgtcta atgaaaaaga aaaggtggaa
     7321 aaagtgagat tttgggctac acaatctcgt gagaaggcac gccattatca acataatgaa
     7381 attggctata attatcgaat gagcaatatc gttgcaggta tcggtcgagg acaattgaaa
     7441 gttttaaatg aaagaattga gaaaaaacga tatattcata atttttataa aaaagaattg
     7501 tctaaattgt caaatataca attcatgcca gaaaataatt ggaatgaacc gaactattgg
     7561 ttaagcgtca taacaataaa ggggataaat acgaatcatc ttattgatga gttagataaa
     7621 aataacatag aagcccgccc agtatggaaa cctatgcatt tacaacctgt tttcaaaaat
     7681 tatgattata ttggcgatga tgtagcagaa actttatttg acttaggagt atgtttacca
     7741 tcagatacaa agatgacaga tgaagattta aaatatgtag tatctacgat taaaagagtg
     7801 atcaatgatg cgtaaaaaaa ctttctatca gctttttggg aaaagaattt tagatatcct
     7861 attgtcaggc attgctctca tcgttttatc gccgataatc ttaattgtag gttttttagt
     7921 aagaattaaa cttggttctc caattatctt caaacaagaa cgaccaggaa aaagtgaaaa
     7981 aattttttcc atgtataaat ttcgtacaat gactgacgaa agagaccaca atggagaata
     8041 tttacctgat gaaattcgac taactaaatt tgggaaaata cttagagcaa caagtctaga
     8101 tgagctacca gaactttgga atattttgaa gggtgatatg tcgatcgtag gacctcgtcc
     8161 attattagta gagtatttgc cactatatag tgagaagcaa cggaaaagac acaacgtaag
     8221 accaggatta actggactag ctcaggtcaa tggaagaaat gcgattagtt gggaagaaaa
     8281 gtttgattta gatgtttatt atgttgataa aattagtttc tttaatgatc ttattattat
     8341 aattcagaca tgtaaaaaag taatcaagaa agagaatatt aatacaatca ataacaatat
     8401 accagaaaaa tttaaaggga gttaaatata gtatgccatt aaatatttta tttcttacgc
     8461 ttttagattt tcaaagttta gaagaaagca atatctatac tgatttgatg tctgaatttg
     8521 taaaaaataa tcacaatgtg tccattatat ctccaacaga aagaaaaaaa agaataaaaa
     8581 ctcatttgat cgagataaat gataaatgtg aaatcttaaa attaagaata ggaaatattc
     8641 aaaagactaa tttaattgaa aaaggttttt ctactataca aatagagaga aaatttatat
     8701 caggcattaa tcaatatttt tcaaacaaaa aatttgatct ggttatttat tctactccgc
     8761 cagtaacttt tgctaaagtg gtgaagttta taaaaaaaag ggataattgt aaaagttatc
     8821 ttttactaaa agacatattt ccacaaaatg cagttgattt aaatcaactc tctaaaaata
     8881 cttatatata taagtatttt agaaaaaaag aagtttctct atatgaagta tctgattata
     8941 taggaacaat gtcagaacaa aattctcaat atataagaaa acataataaa attgatgagc
     9001 aaattttaga agtaaatcct aattcaatta gaataaacga aaataaaatt ggatcccctc
     9061 tagacaaaga ggaaataaga aatttatata aaattcctaa agaaaaaact ataatgttat
     9121 atggaggaaa cctagggaaa cctcaaggta tcgactttct tttaaatatt ataagtagcc
     9181 tagaaaaaga taacaaaatt ttattgctga ttattgggtc aggaacagaa tatgggcgta
     9241 taagaaaaca tataaatact aataatataa aaaatacaat tctaattgat agtttatcta
     9301 aaaacgaata taaaaaagtt gaatccattg cggacatagg attggtattt ctagataaaa
     9361 aatttactat ccccaatata ccttctagaa tattaggata tatggagtca aaaaaaccta
     9421 ttttagctgc tgtagacaag catactgatt taaatgaaat gataaagact ggaaaatttg
     9481 ggtattgttc tgaacatgga gatttaaacg catttctaag taatatggag cgattaaagg
     9541 atgaaaaaat aagaaacgaa ttaggtaaaa atgcttttat ttatttgaaa aataattttg
     9601 atgtaaaaag aagctattct ataattatga atcattttga attataaaaa ggagcaattt
     9661 aaatgttcaa agataaaatt ttattaatca caggcggcac gggttctttt gggaacgcag
     9721 tgatgaaacg ctttttagat tcagatatca aagaaatacg tatcttttct cgtgacgaga
     9781 aaaaacaaga cgatatgcgg aaaaaataca ataatgacaa attgaagttt tacattggag
     9841 atgttcggga catcaacagt gtcaaaaatg cgatgtacgg cgttgattat gtcttccatg
     9901 cagcagcact taaacaagta ccttcttgtg agtttttccc tatggaagct gtgaaaacaa
     9961 atatcatggg aacagataat gtcgtaactg cagcaatcga aatggggatt caaaaagtta
    10021 tttgcttgtc cactgataaa gctgcttatc caatcaatgc tatggggatt tctaaggcta
    10081 tgatggaaaa agtatttgtt gctaaagcaa gaaccgttac tgacgaacgc acgatgattt
    10141 gtggcacacg ttatggaaat gtaatggctt ctagaggatc agttatacca ttattcattg
    10201 accaaataaa aaatggtaaa ccattaacag tgacagatcc tggcatgaca agatttttga
    10261 tgagtttgga agaagctgta gagttagttg tttttgcctt ccaaaacgca aaagcagggg
    10321 atatcatggt tcaaaaatca ccagcttcta ctgttggcga cctagcgcaa gccatcaaag
    10381 aattattcca tgcagacaat gagattcata tcatcggaac gcgacatggc gaaaagcgtt
    10441 atgaaacact actgacaaaa gaagaatact tagttgctca agatatgggt gatttctatc
    10501 gtgttcctgc agatacacgt gatttgaatt atgacaagta tttcgttgaa ggaagcaaag
    10561 agttgacaca agaaggagaa tacaattcag ataatacaca acgtttatct gtagaacaaa
    10621 tcaaagaaag acttcttgaa ctggactatg taagaaaaga attgaaagag tggcaaaatc
    10681 aatgaatatt ttagtaactg ggtcaaatgg ttttattgga aaaaatttag ttgctgaatt
    10741 aaaaaatgaa ggattcacag acattcttag ttttgataga gacacgcctg tacatttatt
    10801 ggatgaatat tgcgaaaaag cagactttgt ttttcattta gctggagtga acagaccaaa
    10861 agacgatgct gaattcatga caggaaattt tggttttact tcagaattgc tagaaaaact
    10921 aaaagagcat ggcaatacat gtcctgttat gatttcctct tctatccaag cagaacttaa
    10981 taattcttat ggcaagagca aaaaagccgg agaagatcta ttaaagaaat accaaaaaga
    11041 aacaggagca cctgtatata tttatcgctt ctcaaactta tacggtaagt ggtcgcaacc
    11101 aaattataat acagttgtag ccacattttg ttacaaaatc gcaagggacg aagaagttac
    11161 ggtaaatgat ccgtctgcta tcatagagct ttgttatatc gatgatgtcg tgaaggaact
    11221 actccattgt ctgaacggac aaccttatac agaaaatggg tattgtgctg ttcgtgaaac
    11281 ggatgaagta acggtcggcg aattaaaaga actaattaca tctttcaaaa atagtcgaac
    11341 gaatttgagt gttcctaatc ttgcgagaag agtagaaaaa aatctgtaca gtacgtactt
    11401 aagcttctta ccagaagacg aattttctta ttttctaaaa atgaacgtag acaatcgagg
    11461 atcgtttact gaatttttaa gaacaaaaga ccgcggtcaa gtatctgtca atatttccaa
    11521 accgggaatc actaaaggaa accattggca tcatacaaaa aatgaaaaat tcttagtagt
    11581 aagcggagaa ggtgtaatcc gttttagaaa agtagactca aatgaaataa tcgagtattt
    11641 tgtttcagga gaaaaattag aagtagttga tattccagta ggttatacgc acaatatcga
    11701 gaatctggga tcaacggata tggtgacagt gatgtgggta aatgagccat ttgatccaga
    11761 acatcctgat acttattatg tggaggtcta atcaatgaaa aaattaaaag tgatgacagt
    11821 agtaggaacc cgaccagaaa tcattcgttt gtctgctgtt atcaaccgtt tggatcgatc
    11881 agaagcaatc gatcatattt tagtacacac tggccaaaac tatgactatg agctaaatga
    11941 agtattcttc gaagacttca agctaaagaa acccgattat tttctaaatg cagcaactgg
    12001 tacagcgatt gaaacagttg gaaatatttt gatcaagatc gatccaattt tggaagaagt
    12061 aaaaccagat gcattcttag tattagggga tacaaatagc tgtttatgtg caattgcagc
    12121 caaacgtcgc catattccta tttttcatat ggaggcagga aatcgctgtt tcgaccaacg
    12181 agtaccggaa gaaacaaatc gtaaaattgt tgatcataca gctgatatta atctaaccta
    12241 tagcgatatc gcgagagaat atctcttacg tgaaggatta ccagctgatc gtgtaatcaa
    12301 aacaggaagt ccgatgtttg aagttctcaa ttcaagaaaa gatgacatcc aacattccga
    12361 tgtccttaat cgcttagggt tgaaagaagg acaatatttt gttgtttctg ctcaccgcga
    12421 agaaaatatc agctcagaaa caaatttctt acacttggta gatagcttga atacaatcgc
    12481 tgaaacttac caattgccaa tcatcatcag tacgcatccg cgaacaatga agatgatcga
    12541 agcaaaagaa atcaaattcc atgagctgat ccaaacaatg aaaccaatgg ggttcaatga
    12601 ttacaataaa ttgcaaatca atgcaaaagc tgtactaagc gacagcggaa cgattagcga
    12661 agaatcttcc attttgaatt tcaaagcatt aaacatccgt caagcacatg aacgaccaga
    12721 agcaatggaa gaagcaagtg tcatgatggt aggattagaa aaagaaagaa tcatgcaagg
    12781 actggaagta ttggaaacgc agcaaaaaga tactttgcga cacgtcgcag attattcaat
    12841 gccaaatgtt tcggaaaaag tattgcgtat catcctttcg tatacagact atgtgaatag
    12901 agtggtgtgg ggaagatgag aattgttgta aatgatatcg ctgcttcaga aggtggggca
    12961 atgtcagtat tgagagactt acattctgat ataataagta gtggtgatga aaatgaatgg
    13021 atttttttac taggtgaaaa attcatagaa gaaagtgaca atataaaggt actaactttt
    13081 cctaaaataa aaactagctg gattaaaaga ttggattttg attttattag tggaaaaaaa
    13141 attatcaata aattacagcc tgatatctat ttatcattac aaaatacagc tactattggt
    13201 gtaaaagctc ctcaatatgt ttttttacat caaccactac catatcaaaa ggaaaaaaat
    13261 ttttcgttta taaaaaaaaa agagtttaaa atggctacct atcaacatgt tatcggaaag
    13321 atatattcta tattgtttaa aaaaactaaa gctactatta tagtgcaaac tcaatggatg
    13381 aaggaaaatg tttccagtat attaaacaat aaaatactta aggtagatac aaaaataaat
    13441 attgatgaaa atatcatcaa taaacaaata aatgaaaata aaagaaatat gaaacatttc
    13501 ttttatccag catcaaatat tccgtataaa aaccatgatt ttcttataga gagtttttca
    13561 aagtgtaaag ataaagaatt aagacttttt ttaactttat ctagagatga aattgatatt
    13621 aaagatgata gaattatatt attaggtaag gtacctagag agaaagtttt tgacatatat
    13681 agtaaatcaa atttaatatt tccctcatca attgaaactt taggcttgcc attattagaa
    13741 gcacaatgtt ttatgagtcc aattctagct ttagaaacag aattctctaa agaagcattg
    13801 tccggttacc cccataagta tctatttaaa ctagataatt tggacaattt aagtcattta
    13861 atagagaaaa taagtacaag tagtatagta tttaacgttg agaacgtcat ttataagaaa
    13921 gaaaatagag tttctatctt agatatatta aaaggagaga atatttattg aagaaatttg
    13981 tagtagcaca tccagctcaa caacactctt ataaaatcgc tgaagcatta attgaaaaag
    14041 aaatgttatt caaatatatt acttcagttt atttaaaaaa aggaagtata ttgaataaac
    14101 tattaaatat tattccagga gaaaataagt tacgagcgaa aggtcgtcat agtgataaaa
    14161 tatgtgatga ggaggtatta ttaattaatg aattttcagg gttgctactt cttattttac
    14221 agagggtgaa atttttaaaa catttataca aaaaatattg ggatttccat aataaaaagt
    14281 ttaataaaaa attgattaaa tatataaatt cgaatattga tgagatatcg ggagtaattg
    14341 tttttgatca ggtatccttt gatttcttta aaaaaaagat taacattcca ataatactag
    14401 acatgtctgc acctagtgtt gtatttatga aaagtcaatt tgaaaaatat ggaatatcaa
    14461 cattgaaata tgacgcactc ttaaaaaaga ataatttcga aatcaatagt gcagattttt
    14521 tcattggggc ttcttcaata acagaaagat ccttattaaa tatgggaatt gaaaaaaaac
    14581 aaataaaaat tttcccttat ggagtgaata tagttaagga aaatgataaa ttaatgaatc
    14641 taagaacaag aaaaaaaaga ttaaaatttt tatttgtggg aagagtaacc ttagaaaaag
    14701 gaattggggt tttagatagt ttttttgaaa aatttggaat tgaggatgca tggacatttg
    14761 ctggcgatta ttcagattct aaaaaatttt atgaaaaata tgaagataag gtacaatttg
    14821 tgggacatgt atcaaaaata aaaatgtttg aactgtacag ggataatgat gttttagtat
    14881 ttccttcttt agcggatggt tttggtttag ctgttattga agcattaagc aatggaatgt
    14941 atgttatatg ttcagattct acaggtgcta aagatattat tatggaaggc aaaaatggag
    15001 atgtgtttct tacaggagat gaaaatgatt taaataataa aatacaatat tataagaaaa
    15061 atataaataa gattgatcgt ttaaaaatta aagaaactgt aaatgattta acatgggaac
    15121 gttataacaa aaacattata aaatcattag aatattttac cgtaggtatg aacaatgaat
    15181 aaattattaa tgatgttaaa ctttttttat ctctacgtat acatgattga cgcatacggt
    15241 ataataaata atgatttata tctaagaatg gttttgatta tcttatttat aatgataggt
    15301 ttaataaatt gtataaaaga catcatgaat aaaatataca gtaaagataa tctttttttt
    15361 acgttgtatt taatctattt tctaagtacg caatatatgc tttggaatat tgatatatct
    15421 cagctaataa tgattatcta tactccaatg atgtttatag ctttttctag gtttcatatc
    15481 gacagatact tttatgagaa atttaccttt agttatattt tgcttatgac ttttacttac
    15541 ttttatactc gtttttttct taatataaat aatggtttag tgataaattc ttgttattat
    15601 ttgttattaa ctgttcctat agtttatttg tttgttaatc cttatagaaa aaatattagc
    15661 tttttattaa ttagtatcat aattttattt tcaatgaaaa gaacagcatt gataacatta
    15721 gcactaatta tttttattga gttaatttta aatgttttga ataaaaaaag aattagttta
    15781 agaagattag catttcttat accgtttagc ctttttattg cagcattgct aattaaaata
    15841 aacaaaataa tatctagttt gtatgataat aatatattta tgcgatttta ttctggagat
    15901 acaataggcg aatctagagg tgacatttta aaattattat tagaaaaaat atgtacgaac
    15961 tcaaatatat attttatttt attaggacaa gggatcaact caactatata ttatacaggt
    16021 ggtttaacag cccataacga ctttctggag gttttatttg attttggcat ttttggtcta
    16081 ttattatata ttatgattta tataattttc ataaaaaaaa taaggattat tacaaacgta
    16141 aaagacaagc gatcattgtt atatactatg gtcatcttaa tgctagtttc cgtaagtagt
    16201 catctaatat ttattcctag ttatgtaagt ttattaataa tgaattataa ttatctgaaa
    16261 aataaaaatt acttttcaaa ggagaactaa tgaagaaaat tggtatttta acatttcata
    16321 gagctgaaaa ttatggagct gtattgcaga catatgcttt acaggaatat attagtaaaa
    16381 tgagtagtaa ttacgaagta gaggtaattg attataggtc accatttata gaaaaatact
    16441 ataaattact aagttttaaa gcatttacta atattaaaag tcttaaaaga atagtatacg
    16501 ttttattata taatggtacg ttaaaatcaa aaagaaaaat ttttgataat tttattgata
    16561 aatttattcg tgttagctta tcagagtatg atgataaaac aataagaggc ttggatgata
    16621 aatatgatct ttttatctgt ggaagtgatc aaatttggag tccatactcg gctggtttcg
    16681 atcccatata ttttttagat tttgtaaaaa aaaggaataa aaaaaatgct tatgctgcta
    16741 gttttggagt agaaattgtc ccggaagaaa ctataaaaga gtatagtcga cgtttaaaaa
    16801 actttaatgt aatatcagtc cgtgaagaga ttggaaaaaa aattattaaa aacttgattg
    16861 aagaagatgt acctgtatca cttgatccaa cactattact tagtaaaact gagtgggatt
    16921 taatcattga gaaaaaaaga aaaatttcag agaaatattt attgatatac ttaattacag
    16981 aaacaagaga aattattgag ttagcaaaaa aagtagcaag agagaaaaaa ttaaagttag
    17041 tatatataaa cgataatatc tacaaaacaa agggaatata taattataat aattgttcac
    17101 ctagtgattg gctcaattta ttttataatg cagattatat tgtaacaaat tcatttcatg
    17161 gaattgcttt ttctataaat tttgaaaaag aattttctgt acagtattta cctgaaccag
    17221 cgaaggttaa ttccagaatc aataatattt tagataatta tagtttgaaa tggagaatta
    17281 actatgatgg taatgagaaa attaattata gtaaagtaaa caaaatactt gtagaagata
    17341 gagttgcctc ccaacaaata ctaaaagata ttatggaata ttgaggtggt attatgaagc
    17401 caaaagtatc tataattatt cctgcatata atgctgagta tactatttcg agagctctcg
    17461 aaagtgcact taaacaaact tattctaata aagaaataat agtcattgat gatgggtcta
    17521 aagatagaac attagcaatc tgtaaagagt atcaattaaa taatcatgaa atatttatat
    17581 tctcacaaaa aaattcaggt gtatcagtag ctagaaattt gggaataaga aaagctgatg
    17641 gagaatttat acaattttta gatgctgacg atgctatgga acctaatatg actgagaaac
    17701 ttattgaagt tcagcgcaat aatgattctg actatgtaat ttgtgggtta actattataa
    17761 aagataataa tatagtgaga accccacatc taaaggaaag aaatatcaat tttattaatt
    17821 ttgaagatta taaatatatc tatcctttgc ttgctgcacc atgcaataaa ctttttaaaa
    17881 aggagcttat tgtactattt gaaaaaaatt ttagcaatgg agaagacttg ttgttcaata
    17941 cggaatattt aacaaaaata aaaaaaattt caatcattga agactgttta tataaggtat
    18001 acttagataa tgaaaattct ttaaacaaaa aaatgagtat atctactgta aaatcgttat
    18061 tattaatcaa tgaatatgaa aaagaaataa tgaataaatt atttgataat gataatgttc
    18121 ttgaatttaa tgtttttaat aaatttaaaa tcatacagac gtctttacaa aaaatagtta
    18181 acaatgaaga aaaagatgat gttatagaat tgataaataa tttaaggtca gatagtcaat
    18241 ttctaacgat attagaaaaa acaaacatga aaaacatgaa aagcaattta atcaaatttt
    18301 gttttagtta ttgtgatagc caaacaattt atcagttgtt ttttttaatt agtaaagtac
    18361 gaggttactt tcaatgaaag atagagtaaa aaatgtaatc tataatgcag tatatcaagt
    18421 atttataata gttattccta tacttactat gccatatgta tcaagaaagt taggggcaga
    18481 aaacttaggt ataaatacat atactcaatc cctagtattg cttttaggat tatttatcaa
    18541 ttctagcatt ctacaaattg gcgcaagaga aatcgctaga caggatagaa ttagtagaac
    18601 aaaaacattt attacacttt ggttattaca agtactatgt gggttggtta ttatcatagt
    18661 gtatatatta atcattatgg gtagcaaata ctattattat tatttactac aattaccttt
    18721 tttaattagt tatatatttg atatatcttg gttctttgtc ggattagaaa aaataaaaaa
    18781 tgttgtattt agaaatatgt tcgtaaaggt atgtacaatt tttttgattt ttctattgat
    18841 aaaaaataaa aatgatcttt ctatatacat cattattatt tctttgggat cgttagttgg
    18901 taattttata tttttcttct ccttaaaaaa atatctcgat ttcgacaaga ttgtcggttt
    18961 agatttatct aacttaaggc catttattaa ttcaattgta atattgcttg ttcctcaagt
    19021 ggctattcag ctatatcagt atataataaa atttagtatt gaaaattcta ctacgactac
    19081 tgagttatct tattttgatc aatctcaaaa aattgctaga ataatcattc aattagttag
    19141 ttcaattagt attgtattaa tgccagtcat tgcaaatcta gataaaaata aatccagaaa
    19201 tgctctgaat aaaattattc agttgtcggt aaattattca ttaagtatag cggtgatatt
    19261 tacaattttg cttgtaataa tttcacccac tttcgttcca attttttttg gtcctgaatt
    19321 taaagaaatg atacctcata tgataatctc atcttttatt ataattccaa tttgtgtagg
    19381 tggagtcttt tctaaccagc tcattttagg gatggggttg tataaatatt atgctacacc
    19441 atatcttata ggtggaagta taggaatttt aatttgctta atttataaag acaaaatgag
    19501 tggattaatt ggatgtatag tactagttat agtagaatca cttgtttgct tttttagaat
    19561 aatatttgca aataaaataa tagatataaa agaaaattta caagggacaa taaaggaatt
    19621 cttcttatta ttcttattat tagctgaagt atacattgta aataccatat ttacgccaag
    19681 cccatcagcg ttattaaatt tattttatat gatgatcata attatacaat actttgtttt
    19741 aagtactata ataaaaacag agactgcgaa agatttatca aaacggttaa aatcattttt
    19801 aattaaataa cactttacca aactaaatct ggaatagaag tttatgcgta agcgccccat
    19861 agaacagtac cttactaaca aatgcacctc aagatatact tgagctatca agaagtctgg
    19921 aggttttcta tgtataaatt aaatgagatt gttcctgtcc aattaggaaa cgaaaagagt
    19981 gcaccagaaa aaggtaaacc cgcggctgcc aagttagcga tgcgcatcaa aagagagaat
    20041 ggttctgaag ttttgatcta taacggtatt aacaattata
//
LOCUS       DACECR010000048        19916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.048, whole genome shotgun sequence.
ACCESSION   DACECR010000048 DACECR010000000
VERSION     DACECR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.048"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(59..187)
                     /locus_tag="IWO17_001617"
     CDS             complement(59..187)
                     /locus_tag="IWO17_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0290500.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IWO17_001618"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IWO17_001618"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0290501.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="IWO17_001619"
     CDS             complement(1674..2729)
                     /locus_tag="IWO17_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0290502.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IWO17_001620"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IWO17_001620"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ0290503.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IWO17_001621"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IWO17_001621"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ0290504.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IWO17_001622"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IWO17_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ0290505.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IWO17_001623"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IWO17_001623"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ0290506.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IWO17_001624"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IWO17_001624"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ0290507.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="IWO17_001625"
     CDS             10491..12050
                     /locus_tag="IWO17_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ0290508.1"
                     /translation="MKKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="IWO17_001626"
     CDS             complement(12161..14092)
                     /locus_tag="IWO17_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0290509.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IWO17_001627"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IWO17_001627"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0290510.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="IWO17_001628"
     CDS             complement(15021..15773)
                     /locus_tag="IWO17_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0290511.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="IWO17_001629"
     CDS             complement(15932..16141)
                     /locus_tag="IWO17_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290512.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="IWO17_001630"
     CDS             complement(16152..17480)
                     /locus_tag="IWO17_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290513.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17845..18432)
                     /locus_tag="IWO17_001631"
     CDS             complement(17845..18432)
                     /locus_tag="IWO17_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ0290514.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IWO17_001632"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IWO17_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ0290515.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
BASE COUNT         5293 a         4284 c         3585 g         6754 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atgaaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaa
//
LOCUS       DACECR010000049        19705 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.049, whole genome shotgun sequence.
ACCESSION   DACECR010000049 DACECR010000000
VERSION     DACECR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.049"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(216..446)
                     /locus_tag="IWO17_001633"
     CDS             complement(216..446)
                     /locus_tag="IWO17_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290516.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="IWO17_001634"
     CDS             1063..1725
                     /locus_tag="IWO17_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290517.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="IWO17_001635"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="IWO17_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0290518.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="IWO17_001636"
     CDS             5145..5459
                     /locus_tag="IWO17_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0290519.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="IWO17_001637"
     CDS             5472..5846
                     /locus_tag="IWO17_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0290520.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="IWO17_001638"
     CDS             5856..6200
                     /locus_tag="IWO17_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0290521.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="IWO17_001639"
     CDS             6271..7623
                     /locus_tag="IWO17_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0290522.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="IWO17_001640"
     CDS             7683..8825
                     /locus_tag="IWO17_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAQ0290523.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="IWO17_001641"
     CDS             8850..9104
                     /locus_tag="IWO17_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290524.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /locus_tag="IWO17_001642"
     CDS             9360..10544
                     /locus_tag="IWO17_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0290525.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10559..10678
                     /locus_tag="IWO17_001643"
     CDS             10559..10678
                     /locus_tag="IWO17_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0290526.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            10683..10904
                     /locus_tag="IWO17_001644"
     CDS             10683..10904
                     /locus_tag="IWO17_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290527.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="IWO17_001645"
     CDS             10917..11417
                     /locus_tag="IWO17_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0290528.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13372
                     /locus_tag="IWO17_001646"
                     /pseudo
     CDS             11514..13372
                     /locus_tag="IWO17_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
     gene            13375..14271
                     /locus_tag="IWO17_001647"
     CDS             13375..14271
                     /locus_tag="IWO17_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0290529.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14321..14710
                     /locus_tag="IWO17_001648"
     CDS             14321..14710
                     /locus_tag="IWO17_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0290530.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14697..16199
                     /locus_tag="IWO17_001649"
                     /pseudo
     CDS             14697..16199
                     /locus_tag="IWO17_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            16944..18770
                     /gene="ltrA"
                     /locus_tag="IWO17_001650"
     CDS             16944..18770
                     /gene="ltrA"
                     /locus_tag="IWO17_001650"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0290531.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18904..>19705
                     /locus_tag="IWO17_001651"
     CDS             18904..>19705
                     /locus_tag="IWO17_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0290532.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         6662 a         3220 c         4309 g         5514 t
ORIGIN      
        1 gatatcaaac aaaaagtata cacccttctt cattctagtt ttcgcagcag gaattttgaa
       61 atagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctaaaaagaa
      121 aacttctgaa ttccatactt tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaga ggtatcaaaa acttagtgaa gagttatctc aagatgaatt aaaaatgtta
    13381 caaaaccttg attctttata tcaatcttta tcaaaaactg aagagcgatt ggaagaaatt
    13441 attcaacaag aaataaagct taaagattac caagttgagg ttgactcagc aatgtcaaga
    13501 atgaataaat catatcaaaa tttctattct gagagaagta gttcctcgcg cgaaattttt
    13561 tctgatatta caggaataga ggctactatt caaccaaata gggatttcaa taattatgtt
    13621 aaagagctca gagcttgtat taataaggag gacacttttg acagtgagtt tggatctctt
    13681 attgataagc tgacaaaaaa ttccattact aatcaagata tatataatga catacttgat
    13741 ataagagtta gtggggaaag tagtattttt tcagataaaa aattaatcaa ttctataaga
    13801 aaattgaatg atgcacaagt tcttgaatta caaaccctta agccaaatga tttagtagtt
    13861 atccgacttg aactaaatgg aaaaaaagtt agtttatcga atgctagtgc tggtcaaaag
    13921 acatctgcaa ttctttctat gattttggcg tatggaaatt caccactcat attggatcag
    13981 cctgaagatg atttggatag tcaattaatt aataacttaa tcgtaagaag tatcattaca
    14041 aaaaaggaaa acagacaaat tttaattgta actcataatg caaatatccc agttaatggt
    14101 gattcagaat ggttgattag catgggagat tcaaaagaaa tatcggctga tcagtgcggg
    14161 tcggtagatg aacccaatat caagccaaga atatgtactg tgatggaagg tggcgaggat
    14221 gcttttacaa atagagcgaa acgctatggt tttaaacaaa ttgtagaata acactagagg
    14281 gtgagacata ttattatgtc cgcccttttt aggaggaaaa atgaaaaaaa taaaaagcta
    14341 taccagtatc tgggcagttg aaaaagtcat ttacgcaatc aatgattttc agttgccttt
    14401 tcctgtgact tttaaccaaa tggcttggtt cgttttatct ttactgtttg tcatcgtatt
    14461 tgcccatgtg cctccactat ctatgataga ggaggtcttt ttaaaatact taggcatacc
    14521 ggtggctgtc acttggttta tgtcgcaaaa gacgtttgat ggcaagaaac cactgggatt
    14581 tttacgatcc tttatcagct atcacttgcg atttaaggtg acttttgcag gaaagaaagt
    14641 caaagaacag aagaaacgat gggatgaacc cagtactctt gtaaggagtg tgaactatgt
    14701 atccgattaa gtatattgaa aataatctcg tctttaacca agaaggcgag tgttttgcct
    14761 actatgaatt agtgccttac aattactcct ttttgtcacc cgaacagaaa tttcaagtgc
    14821 atgataactt tcgccaacta attgcgcaaa atcgtgaagg caagatccat gcgttacaaa
    14881 ttgccacaga aagtagtatt cgcgccacac aagaacgatc aaaaaaagaa attaccggta
    14941 gactcaaaga agtagcgaaa caacgaattg atatacagac agatgcatta gtatccatga
    15001 ttggcgatag tcagattgat tatcgctttt ttattggctt taaactaatt gcgacagatg
    15061 aagaagtcaa tctaaagagt ctcaagaaat ctttcttttc tgggcttcaa gaatttgtct
    15121 atggagtgaa tcatcatttg atgggcgatt ttgtttcctt atccaatgaa gaaattcgct
    15181 gctattctaa gcttgaaaaa ttaatggaaa gcaaattggc tcgaagattt aaagtcaggc
    15241 gagtcacacc gagtgacctg acgtatttga ttgaacatat ttatggcgaa aaagggatac
    15301 cgtttgaaga atacgaattt cagctaccaa agaaaaagct aaaatcagaa acactggtga
    15361 aacgttatga cttattacgt ccaaatcgtt gtttaattga agaaaaacca aggtgcttac
    15421 ggatggaaca tgagaatcat gagtcgtacg tagcgtattt aacaattaat acaattgtgg
    15481 gggagatgga atttccttcc tcagaacttt tctattacca gcaacaacaa tttacattcc
    15541 caatcgacac aagtatgaat gtagagattg tgacgaataa aaaggcttta gctaccgttc
    15601 gtaataagaa aaaagaactg aaagatttag acaatcatgc ttaccaatct gacaatgaaa
    15661 cgaattccaa tgtcttagat gctttggatt ccgtggatga attggaaacc accttggatc
    15721 agtcaaaaga atctatgtac aaactgagtt atgtggttcg agtgagtgcc gaatcagtgg
    15781 atgaactaaa gcgacgctgt gatgaagtgc tcgattttta tgatgatacc aatgtaaaac
    15841 tagtccggcc ctttggcgat atgatggggc tacatgaaga attcttaccc ttaagtaaac
    15901 gatatatgaa cgactatatt cagtatgtta cgtctgattt tttggcgggg cttggttttg
    15961 gggctactca gatgctagga gaattagaag ggatttactt tggctacaat gtggatacag
    16021 gtcgtaatgt ttacctgaaa ccggcattgg cttcacaagg agtcaagggt tcagtaacca
    16081 atgcgctggc cgccgctttc ctgggttctc ttggtggagg taaatccttt agtaacaatc
    16141 tcttagtcta ctatgctgtt ttgttcggtg gtcaagctgt gattgtcgat ccaaaagggg
    16201 gtgcgaaacg ttcctacttg aaaagagtag gcacagcact tcattaaatc aatatttaat
    16261 gtgtgttgga gaactaaaca cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta
    16321 ctttaatact ctgactggca gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa
    16381 gtcggcagac agacaccgta acagataatg ctgacgaaag attcccagag gtgggcgttg
    16441 tgtcctatat gccggaagtc tataaaatat ctatggttag aatgtagatg agaggtctac
    16501 tgacgaatcc ttgaatgtac aggtctatat cggggctgga tagaaatgtc cagtgacaca
    16561 aacagtgtcg ctactgtggg tgagtaagac tccgcattta tgaaaatcca tataacgtta
    16621 aaggcgttat caaggtagca ggcttataga ggaaacctaa ggatatatgt acagatagtg
    16681 tgtttggaac gttgaaagct gacaacatgg agattttact gtctgtgaag tgttcacatc
    16741 agaaagttat tgtgagatta gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg
    16801 cacagtacct atgaaaccgt gataacaagc ggtggaggga tagccactag tcatatgaag
    16861 aatagaatta ctaagaaaaa tggtatgggt tcgagtaaga ctaagagatg tacttgctcc
    16921 tgcaaacaag gagggtacag accatgctaa agaaaaacaa gctccgctat aacgagtatt
    16981 atgatatgca acatatttat gacgagttat acgcacagag caagaacggt aataactttt
    17041 ataagttact tgaaattatt ggttcggaac aaaatatttg tttagcttac cgtaatctga
    17101 aatcaaacag tggcagtaaa acggctggaa cggatggaat gacgatagac gatataaaac
    17161 aactcagcaa tgctgagatt gttgctaccg ttcgggaaag ccttagcaac taccgcccca
    17221 aatcagtcag acgtgttttc attccaaaag ctgggagcga taaaatgcgt ccattgggga
    17281 ttccgtgtat ctgggacagg ctggtacaac aatgtatcct gcaagtccta gagccgatat
    17341 gcgaaccaaa gttccataat cattcctatg gattccgtgc gaatcgaagt gcccaccatg
    17401 cagtcagcag agtaacgaca ctgataaatt tatcaaaata tcattactgt gttgacgtag
    17461 acattaaagg atttttcgat aatgtcaatc atggaaaact attaaaacaa atctggacac
    17521 ttggtattcg ggataaaaga ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg
    17581 acggcgaggg tgtcccagaa aaaggaacgc cacaaggcgg tctgttaagt ccactgctct
    17641 ccctcatagt ccttaatgaa ttagactggt gggtaagcag tcagtgggaa acattccagc
    17701 cgaagaaccg cagtaaaaat gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa
    17761 gcggttttat tgtacgttac gctgatgatt ttaaaatcat gtgttcaacc tatggagaag
    17821 cacaaagatt ttaccacagt accgtagatt ttcttaataa aaggctgaaa ttagaaatca
    17881 gtccagagaa atcaaaggta gtaaatctga agaaaaattc atcagatttt ctggggttca
    17941 aaatcaaagt cattccaaaa ggaagaacga aacacggtta tgttgccaaa acagatatga
    18001 accaaaaagc attaaagaaa gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca
    18061 gacatacgac tggcttcaat atcagcagat ataatctgac agttatcggt atgcagaatt
    18121 actactgcat agcaactaac gtatataaca acctgacaga agtaagttac gctctcttac
    18181 cgaccatacg aatccgttta aggaacattg caaaatcagt tccatttgaa agtacaagtt
    18241 cagaatttca gagcaggaca aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg
    18301 ataatccgct attgccgatt caaggagtac aacacaaaaa tcctatgaat ttctcgcaag
    18361 atatttgtaa ttttacaaag caagggagaa acaaggtgca tgaggacgtt gtagttgtaa
    18421 caaaagaaga aatccgagcg ttgttggaaa atgaaaaccc tgccgatagc gtagagttta
    18481 acgacaatcg tatttcggca tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc
    18541 gaggaactcc gtccactctg atatgtattc acaaatcaga caaaggagat aaccttgaca
    18601 gatattctaa tctggcattt gtggaaattc ccattgcaaa ggcaatccta acagaatcag
    18661 cagacgaagc aaaatcactg ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac
    18721 tgaacagaat acggacgaat tacggatacc agacaataac tgggaaataa cttataaatc
    18781 tatttggata tgatggaacg ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg
    18841 gaaaacttgg agataacatc aaaaagttac ctatcgctat agcgaggagg gtggaaagaa
    18901 accttgccag aaattgcgga tgaaatcagt atcctcaact tgacgagtaa gtcagaaaat
    18961 caaggtctgc ttgatcctta tgtgattatg aagaaaaaga aagactcgga aagtttagca
    19021 attgatattc tgactttttt aacaggaatt tcaagtcgag acggggagaa gtttcctgtg
    19081 ttacgtaaag ccattcgaac ggtgactcaa agtgatcaac gggggttatt acaagttatt
    19141 aacgaattgc gaaaagacgg gtcaccaatc gcggaaaata tcgccgatca tatcgagtcc
    19201 tttaccgatt atgattttgc tcatttgctt ttctcagacg gctcgattac ccaatcgatt
    19261 agtttagata aacagctaaa tatcattcaa gtggcggatt tggttttacc ggatgcagaa
    19321 acgacttttg aagaatatac caccatggaa ttgcttagtg tttcgatgct aatcgtcatt
    19381 tccacttttg ccttggactt tatccatagt aaccgagaaa tcttcaaaat tgtcgatttg
    19441 gatgaggctt ggagttttct tcaagtggct caaggaaaaa cgttatcgaa taagcttgtt
    19501 cgtgctggtc gggccatgaa tgctggggtc tatttcgtca ctcaaaatag cgacgattta
    19561 ttagatgaac gtttaaaaaa taatatcggg ttgaagtttg cttttcgcag taccgatatt
    19621 catgaaatca agaaaacctt ggaattcttt ggtttggatc aggaggatca aagcaatcaa
    19681 aaacgcctgc gagatttgga aaacg
//
LOCUS       DACECR010000050        19677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.050, whole genome shotgun sequence.
ACCESSION   DACECR010000050 DACECR010000000
VERSION     DACECR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.050"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(44..1693)
                     /locus_tag="IWO17_001652"
     CDS             complement(44..1693)
                     /locus_tag="IWO17_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0290533.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(1810..4413)
                     /locus_tag="IWO17_001653"
     CDS             complement(1810..4413)
                     /locus_tag="IWO17_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0290534.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(4425..5474)
                     /locus_tag="IWO17_001654"
     CDS             complement(4425..5474)
                     /locus_tag="IWO17_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAQ0290535.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(5500..5814)
                     /locus_tag="IWO17_001655"
     CDS             complement(5500..5814)
                     /locus_tag="IWO17_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAQ0290536.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(5830..6276)
                     /locus_tag="IWO17_001656"
     CDS             complement(5830..6276)
                     /locus_tag="IWO17_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0290537.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(6277..8253)
                     /locus_tag="IWO17_001657"
     CDS             complement(6277..8253)
                     /locus_tag="IWO17_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ0290538.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(8411..9307)
                     /locus_tag="IWO17_001658"
     CDS             complement(8411..9307)
                     /locus_tag="IWO17_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0290539.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(9300..9980)
                     /locus_tag="IWO17_001659"
     CDS             complement(9300..9980)
                     /locus_tag="IWO17_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0290540.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(9981..10763)
                     /locus_tag="IWO17_001660"
     CDS             complement(9981..10763)
                     /locus_tag="IWO17_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0290541.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(10756..11673)
                     /locus_tag="IWO17_001661"
     CDS             complement(10756..11673)
                     /locus_tag="IWO17_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0290542.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(11837..12160)
                     /locus_tag="IWO17_001662"
     CDS             complement(11837..12160)
                     /locus_tag="IWO17_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0290543.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            12948..16175
                     /gene="fss3"
                     /locus_tag="IWO17_001663"
     CDS             12948..16175
                     /gene="fss3"
                     /locus_tag="IWO17_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0290544.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            16288..16602
                     /locus_tag="IWO17_001664"
     CDS             16288..16602
                     /locus_tag="IWO17_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0290545.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            16615..16989
                     /locus_tag="IWO17_001665"
     CDS             16615..16989
                     /locus_tag="IWO17_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0290546.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            16999..17334
                     /locus_tag="IWO17_001666"
     CDS             16999..17334
                     /locus_tag="IWO17_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0290547.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            17417..18757
                     /locus_tag="IWO17_001667"
     CDS             17417..18757
                     /locus_tag="IWO17_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0290548.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            18835..19509
                     /locus_tag="IWO17_001668"
     CDS             18835..19509
                     /locus_tag="IWO17_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0290549.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
BASE COUNT         6115 a         3772 c         3520 g         6270 t
ORIGIN      
        1 aagccaagat ttttgatcca ataaatactt tcgatcactt cgcctatccc aatacatgcc
       61 actgccgctt ttcatcaaat aacgcggctt ccgtttttgc ggtttctact aaagtctctg
      121 ggtaatccaa aactttcagc aaggcaatcg catttcgggt ccttgaaggt ccttctttta
      181 atttgaaatc aaaactgatt ccttttcctt ctgttacttg ttcttcaaag tggacattct
      241 cacaataatt ctttaaaatc tctgtcaatt cgatatcatg tgtggcgaca aatgctaaag
      301 atggatactc cgcaagccac cgaacgactg aagcggaaga agcaatccgt tcgatcgtat
      361 ttgtcccttt taaaatctca tctacgaagc agtagcagcg ttctttggaa gccacttggt
      421 ccaacaaacg tttgatcgat ttgatttctg aaacaaaata actgtcgcct tcaaataaat
      481 catcttcgat tgccatggaa gtcaacacat gtccaggttg cagggtaaac tgttctgcaa
      541 tcgctgtttg gatcgtctgt gctagaatac agctgatggc tatacttttg acatacgtag
      601 attttcctga tgcattggaa cccgtgacta atgtattctt ctgccagttg accggattca
      661 ctaccgcttc ttttaacaat ggatgataga tatctttcgc tgtcacaccg ccctcagtaa
      721 actctggtcg gcaagtgatg ggcatataag tacggtaatt caatattgca gcggctactt
      781 ctaattctcc cagcaattcc cacagcttga tggcttcagt tgaatgcttt tctaatttag
      841 tgagaacgaa atgataggag ataaatggca gcatgaacat gatgttgaca tattcaaaaa
      901 tcaactctgc ttcactccca ctcttcatac gaaatgaaaa acctaattta gggatgtctc
      961 ttaatggttt agtttgctgt ttcagctctt cttgaagtgg tgtcggcagt tttgctagct
     1021 gattggcggc tgaaatcgtt gaaaccaaat aactcatgct attcagctct gtttctagtg
     1081 tttgtttctt cactagataa tagaccgtat tgaatactaa cccaccgatt gccaaataga
     1141 ttcctagtac ttgtcctaaa agaagcaaac ccaaacccac cagcggaagc aagccaagaa
     1201 aaataaataa acctacgcgt ccaatctttt tactttttcc atccgctaga tattgttttg
     1261 caaaattatg atcttttttt cctaaacgag cgaattgata ttggattttt tctctcagtt
     1321 gaggattttc ctgataaaaa gcaatcagtt tttctaactg ctgatcttca ccaaaatcaa
     1381 agctgcgtaa acgttgatac aacgcttctg aacctacact agaataggta ctgttgattc
     1441 cttcgaatac ttcgaacata tccaggtcat accaagtaag atcatcgatt tcgctatccc
     1501 atgaacggaa cttcttctcc gtgagccatg cttccttcaa gctttcttct ttatcaaatc
     1561 tcggttgata aggaattttt tcccatttgc tacgaactgt ttcctttaat ttcaaacgtg
     1621 aaaaaatgtc taaagcaact aataaaataa cagctgcaat aataattaaa acgataaatt
     1681 ctctttgacc catatccctc tccgttcctt tatttgttag ctttagtata gcactgttgc
     1741 gctatttgaa aagagggtaa aaaaacacct ttcaaaataa aaggtgtttt tttcttgtat
     1801 attacatatt tatctctcat ttatcaaaat tgacaaataa tcatagggag gaattgttgt
     1861 taattcttgt ttattctcca gtgcattgac aactgtccca agggaaagga tagcctcagg
     1921 tactattttt tcttgttctg ttggattagc taagcgaaga acaaatcctt gttcatgaaa
     1981 ataagaagga taaatcgatg atacaattaa gtcttcaggt aattcaatca atgaaaattt
     2041 acgaggaaca tcaagttcat tttgtaatgg ccaaatcttg ttatctaacc tgtgaataaa
     2101 tacattcagt ttctgctttt gataagaaat agattgttct aaccgttttt ctgcctgttt
     2161 tgccactttg aactcattaa atcttccttc ttctacatta aatccaaaag tgactttata
     2221 ctcacctatc tcttgagcta gcggtgtttc catcatgaca tgcccttcat ttgtcgtatc
     2281 tcctgaagct cgcccaggtc gccaagcaag attaggtttt ccaagttccc ctgttgttga
     2341 aaataaggtt atgattaatt gattttctat acgttcatac tcttttaaac catctgcata
     2401 aaacgtaaaa taatgatcat tttctgccac agagacactc ttatcgaata tttctaaatt
     2461 aacaggtttc tctaccatag tttctgacca attcggagga atgacatcag gggtattttc
     2521 aataaatccg ttttgaattt gtgcaattga tttttcagat ttatctaaaa cattaattgc
     2581 taagcgcata cgatgcgagt aaacagtatt atgaattttg agtgtgcctt caacgatttg
     2641 actttctccc agactgaacg atatttcata agatatttct tccatttcgg gtttttcaga
     2701 taaacgatct tttaaatctt taggaagttg agcttttccg taaacaacta atgtttcttg
     2761 taaagaatct ttatatactt tagctgtttc taattttaga gatagttctg tatctccttc
     2821 taatggtgaa taatcatacg tatcgccatc atttgctgaa tcgatcaaat gaacgaaatc
     2881 aagatattgt ctatctccaa ctatcaaatt caattgccca tctttaaaag aaagtttata
     2941 tttattgtta cttatctcag ttttgtctat ctcgattgct gtttctaatt tttcattatt
     3001 tttctcaaat tcaacaacag tgtatcctag tgctggcaca tttacattta tttttatgtc
     3061 aagttcataa taagctggtt catcaaaata ttcgaaacct gtagcaatca tccgataagc
     3121 atttttccgt tctggaaaat aaacttcttt ttcaatgata ttatagggac tacctttgaa
     3181 gtgtattttc tttgaatcag ctactacatg cacgattttc gatccactat aattctcagt
     3241 cgttgtgttg aaaacaagga cttcattcga ttttagcttt aatgcatcag ccatccgttt
     3301 tacaataagg ttttcgattc cgtcagctat ctcattagct tctttgattc gatggaaaat
     3361 atcttccgca acattatctg aaacacagcc accaatacta tcatgagcct ggttttgcaa
     3421 aatctttttc cataaccgac gaagtaggcc tttactgata tggataccat ttctttctgc
     3481 aatcagcatg agcggctcaa ctcgttttag aattttttgt tccaatttaa aattttctat
     3541 tttcattttt gttcttactg agccaataga acgatgaaca cgagagtaaa ccggctgacg
     3601 aagttcacct ttatattcag gtaattcttt tttgtttctg ataacttcta cataagaagg
     3661 ataatctgaa ataatattgt ggaatttact gtatttattc aacttctcta cgacttgatc
     3721 aaaatttaaa atcattgact tttgatctac acccgaaggt actaaaatat ctttgttttt
     3781 tccttgcata tgaagcattt caactgcttc atccaacttt tcttttgcaa attcaggaat
     3841 atctttaatt gcgtctaagg tcatcattcc agtcatataa ccaaatggaa aattcatggc
     3901 ataaacaaat ttatctccca atcctttcca gataaaataa ggagatggaa ccatcgtttc
     3961 aaagtttaat cctcgccaac acatgaaatt atcgatttct acttgatgca aaagtgtagg
     4021 caaattcgca ttgaacccaa acgtgtcagg caaataccct accatcattg gatctccata
     4081 tttaaggatc gtatcactga ttccaatcat taagtttctt aaaattgatt cagcatccac
     4141 taataaggca tctgtttgtg cataccacgg tcccacgaat agttgtcctg ttttcatcag
     4201 tcgttggata accttaagct tttgcggatt gatctccaaa tactcatcga cgattgaagt
     4261 ttgtccatct aaacaaaagt ttactttagg gttcctttcc aattcatcta gaacttcagt
     4321 aaagacctga tcagacaata cgatggcatc ttcatttgta aaataccatt ctctgtccca
     4381 gtgcgtgtgt tgaattatat gtgcatttac catatcagat tctcctactt tccttcttct
     4441 actgaattta cttgttgttc ttttttatac ttgatggatt ttagtgcatt gaccattaac
     4501 gctgtaacca ctcccccaat aacaattgat actaaatata aaggtatatt cgtaacaaaa
     4561 ggaattgcca taaaggttga aagcattgtt tcttgagtaa caccaaaaaa tccatttaga
     4621 ccacatgcta ctgcagtgcc caccacgatg cttggtatta ctcgtaaagg atctttcacg
     4681 gcaaacggga tcgtcccttc agtaatcccc acaaggccca taatcatagc tgtctttgca
     4741 ttatctcgtt ccgattcatc ataaagtttt ttgtttaaaa ttgttgctaa aaacattcca
     4801 aatggcggaa tagcaattcc taataacatg gctgtactag gtccaaagct tccgcttgaa
     4861 aaagaagcaa ccccaaaagc atatgcgatt ttattgatag gtcctcccat atcaaacgcc
     4921 atcattcctc ctaaaatagc cgacatcaaa gcagcatttg ctcctgacat gccttctagc
     4981 caattagtca gtcctgttgt cattgcttga ataggcgtag ctagtacata ccataatatg
     5041 attccaacaa ctaatgtagt aacaaaagga atcagcataa ttgattttag actaataact
     5101 tgcatcggta cttttacttt tttcaatgca ttagctaaat agccacaagc taatccagca
     5161 accattcctc caataaatcc cataccaaga ttttgacaca tcatcccagt gataaaagct
     5221 ggtgctagac cgggacggtc agcaattgaa tatgcgatat aggctgaaat catcggtacg
     5281 ataaactgta atcccgtctg accaatcatt cgcaggactc cccagaatgt atccgtctgg
     5341 tcccaaacac ctgtgccacc aaaaatgact gcaatagaag ttagtaaccc tgctgctacg
     5401 acaagcggga tcatatagga aaccccactt tggaaatgat ttagaatttc tcttccttgt
     5461 ttcttgaatt ccattagtaa ctcccccacg atatattttt tatttgttaa ttaatgcaat
     5521 tgcttcatca atcgtctctt caccatcttt tactacctct gaggtcccta ctgttaaaat
     5581 cggtttaccg tcaaagcgac ctcgttctga aacatttgta tctactgcaa atatgacaac
     5641 atctgcctcg gaaacatccg aaattgaaag tttgttttct atccccatag ccccctgtgt
     5701 ttctacttta atatctaacc ctttcttctt cgcaaacttt tctaaattag cttttgccat
     5761 ataagtatgg gcaatccctg cgatacatgc tgttactgct actatttttg tcatatgaaa
     5821 ttcctccttt taaaatgata gagcttttac caattctttt ttatttctcg aatcttttat
     5881 tcttttaaca aactcgtcat ccatcaatct tccagataat tctgctaaaa tacgtaaatg
     5941 attagtacct ttatcagtgt cttttattgc taacaaaaag acgagatcaa ctggttgatc
     6001 atctaatgcg ccccattcaa cagaccgctt taacttggct acagccactg ttgattcaat
     6061 tactgctagg cttttgccat gaggaatggc gatatttttc ccaattcctg tggtcatatg
     6121 gttttctctg tcaaatacgt ctttttgaaa ttgtttgtgg tcagacacaa acccattagt
     6181 ctctaacaat ttcgataatt tttcgactac acttttttta tcagatgcat ttaaatctat
     6241 cactatatta tttatattga ttttttcact gatcatttat aagccttctt tcaatatata
     6301 aactaatttt tcaaacgacg aactcgtttt taaatttgct aataactcat gattcgaagc
     6361 gatttcattg aatgtttcat aaattttatt caattcaggt tttacttttt cattaagtgc
     6421 aaaaaagaaa acagaatgaa caagtgtccc atcccaatca attccttctg gattaacgta
     6481 gacacagatt actggctttt ttataaaatc tacttcgata tgcggcatag caaaatcatt
     6541 catcgctgtt gaagatagtt tttctctttt gattgctccc tctagtatcc cttctttcgc
     6601 gtaattttct tttaccaatt ttttggtaat tatatttaaa acgttcactt gattatcctt
     6661 tatttggtgg gtaaaaaagc aaaatcgttt atctaataaa tttagaaaag catcttctgt
     6721 ttgttcattt aactgtagat actgttttac ttttctgaca tctgccccta acatcaaagg
     6781 actaacaaca ataacaggaa aatttgtatt ttcaatcgga atcgtgctta taacaagttc
     6841 atcagtgact tttctatctt gtagttcatt gatgcttagt acatcttcta tctcaaccat
     6901 atctgcaaaa ccgttattta ttctttgctc taataatttt gatgttccat atccgctact
     6961 acaaactaat atcactttcg ttttgtcagg tttgtaacga tccaaaagtg attgaatatg
     7021 gagtgttacg taagcgattt catcttcatt aaaatgtata cagaatctct cttcgatttc
     7081 ctctaataaa tctacactta tcataaatga ccttttaaaa gaatatttga ttttgtccgt
     7141 ataagggtta tagatattta ctcccaattt gagtctttta actgctgcat tgagatgtaa
     7201 tactagatta ttgatcaatt cttcatcagg ataaaattca tttaatttaa ttcttactaa
     7261 ctcttgtagc tcaacaccca tatctaaata ggaaagcttc gtttgatcta agtcgtgatt
     7321 catagcattt ttttcaatcg aagtaatgtg aatgtcaaca taagcttgtt catctattgg
     7381 cacaataatt gaaaatgctt tttcaatcat tttgattaaa ataagtgtat tctttgagat
     7441 ttcttcttca gaattattac tacgtgataa gaaattgctt gtttcaatcc tttctatcgc
     7501 aatagcaagg tggatagcta aagtttgaaa ttcataatct gtaaatttca attgtgtctt
     7561 attcatgtat cgattaagca attcggctac ttttttcaat atgtccttat ttaagaaatt
     7621 attcgcttca ccaatgaaat tgatattcaa gtctaagaga tctgttttac tgttagatgc
     7681 tgtaaagcca ccccaataat agttcacaac ttctgacatc aaccttcttt tacttttttc
     7741 atcagctttg atgcgcacgc catttcgatc tgaaactaag gacatcccat gcttgtttag
     7801 tctttttcta acatctttca ttgtgctttc taccgtcgac ctgctcacgt ataattcgtc
     7861 agcaagattt tgaatcttta gagaatcatt agtattcaat aatttagcaa acacataagt
     7921 tagacggtcc tccggagagt ctgcgatatt gattttatga ctggataata aatttcgaag
     7981 tgtttctttt tttccaataa gcgaaacgcc ttttttaggt tgaaccaaca tttgtacatc
     8041 tggcattttt tggattattt ctttcaaaat taaaatatct ttgctaattg ttttcgtaga
     8101 tacatataat tgttctgata attctttgta agttgtgtgc tcattttcaa tcaaaaagta
     8161 tagtattttc atttgccgtt cgctaaattc gatgcccatt atcttcacct cctgaataca
     8221 gcttaagtgt acaattatta tctctatttt catacagaag aaagttcttg attaatgtag
     8281 aagtttctaa tattcttgat tatttcaaat caataaaaaa gcctgcaata tttatatcac
     8341 aggctttttt ctctattaaa tatatataag ataatacatt tttctactag ttctcactaa
     8401 tcttgtataa ttaaaccctc cgcaacttga cggagatgga gaagatgccc ccgtctaatc
     8461 tagcggtcat ctctcctccc attccttcca ccaatttttt tgacaggtaa agcccgattc
     8521 cgctagattc cgtatttgtg cggctggtat tttcggtata aaaacgctgg aagacttttt
     8581 caacagaggt ttgcgttcct ggtttgatag gattggccac tgttaatcga atgaatggat
     8641 cctcttttga aaatgtcacc tttgcttgtc catctgcata acgcaataaa ttctgtatga
     8701 tattttgcag cactcgcagc aacatgagcg gatctgctaa tacagttgta tttatcgagt
     8761 gttcagcgaa cgctacctcg atatttttct cagaaaattg ttcataaaaa gagaaaaata
     8821 cttcttccac ctctttagaa aaagaaagcg tatggagatc catttctttt tgcttcgtct
     8881 ctattcgagc aagatcataa aactgatccg tcatactgag taatcttgca acagattgtt
     8941 tgatgatcgt taaatacttc tcccgtttct ccgtatcctc tacctgttcc agtaattgta
     9001 cataaccgtt aatagcagtc aaaggagttc tgagatcatg agaaaggccg acgatcgata
     9061 gtctgatgtt ttcttccatt tcttgaaatg cttgtccttt ttgttcattt tcatccaacc
     9121 atgtattcag tgcatctact aggtccatta gattcttttc tcgaaattcg actgaaagac
     9181 gaccaccata ttcttgtcgt tcagaaagag tggcgatttt cttagttaat ctcttgattt
     9241 ccctttgcag gaagaaaata aaacttccca taaagataac aacgagaagc aacagccatt
     9301 taatcaaatc taaaccccac tccccacacc gtatgaatat acacagcatg tgcctttgct
     9361 agcttccctc gaagattgga aatatgcata ttcactgttt tctcactatc aaaatatggt
     9421 tcttgccaaa ctgtttcata aagatttgct cgcgaaaata cttttttcgg atgttctaaa
     9481 aaaagcttca gcagatcgaa ttctcttcct gtcaaatgga cagccacacc attcacattt
     9541 acatccctcg actctttgtc cagtaagatt tccttgtaat agatgcgttg cgacagatct
     9601 tttacttgga cagaagtctg atgacgaagt tggatgccga ttcgtaacaa aagttcttcg
     9661 atatcaaatg gtttcgtcac atagtcatcc gctcctgctg ccaacaagga aactttatct
     9721 ctttgatcgc tttttgctga aagaacgatg atcggaacat tcgatgtctg tcggatttct
     9781 gctaacaatg cttcacctga aagccctgga agcatgagat ctagaaggat caaatcgaca
     9841 ggatatgtgc ttaacaataa ttttccttcc gtacctgaat aagcatcaag aagtttgtat
     9901 ctctgttcaa gaacctcctc cagtaaggaa tgaacagctt catcgtcttc gataatcaaa
     9961 atagtttcca gcaggaccac ctacttaatg tcagattttt ggaaaataaa cattcctaga
    10021 gctgatgata ccacgattgt tactagactg accagaatca ttttcggtgc ttgcgaataa
    10081 gttgcttgtt gcgcaaagtc cgtatacatg agccacgttt ccgtaaacca ctccgttaga
    10141 aattccaagc ctttgatttt tgcgaaaaca ccgggaatga aggccgttcc catgatataa
    10201 agaaaagaaa gaagcaaact gacactggta cttctagtga tgaatgtcaa agccactgga
    10261 acagataatg tccctaatgt cagcagaagc aatacaggaa atacttttag aacataattt
    10321 ccatagtttt tgattccctc aacttcacca aatccgaaag caagtgtgta agcaagcatg
    10381 gctagtcccg tgatcaaaac cacgccaagt gttgtgatca aaagcgtaac gagaagtttc
    10441 gacacataat aagtcattcg attccgtcct aaagataacg tattgcgaat cgtaccgttt
    10501 tgaaattctt ccccaatata aaagcacgca aatgctgcga aagccagaag aatccatgca
    10561 gccatttttt ctaccattaa aatccctgtt gcaccgttga tgactgtatc cgttgcttgg
    10621 tttaattcat tgccgtattt tgtatgcata taccattcgt tccaagaata aaaaagcgga
    10681 agtacgacca tcagtcctag tgagacccac attttacggc tcttccatac ttttattttt
    10741 tcagcagtta atagattaaa catgtggatt gcctccagtt aattgtaaga aataatcttc
    10801 cagtttttgc ccaactaggt gaataccatc cacaggaact tttgcttcta ccaatgcttg
    10861 attgatggct gctacttggt caattccttc aaaaagctgg ataacacggc tagattgtac
    10921 aaaataatcc ctataaccaa gagagtcgag aaccgttact gcttgttccg tcgccgaagt
    10981 gtcaattttt ataaattgac gagattcttg tgccaattct tctttcgata attctttgat
    11041 caagtgtcct tcatgcaaaa taccatagtg agtggcgatc tgtgacaatt catctagcaa
    11101 gtggctagaa agcaagacgg tgatgccccg ttcttttacc aaacgttgga tgatttctcg
    11161 catttctgcc atccctgctg gatccatccc attcgtcggt tcatccaaaa tcaaaaattc
    11221 tggattcgtg atcagtgcat ttgcaatagc taaacgctgg cgcataccta aagagaaatt
    11281 ctttgctttc tttttccctg tattttccaa tcgcatcatt ttcagcaggt cttcgatttc
    11341 tctatccgac acaccgccgt tcgctgcttg gatacgtagg ttatctcttg cagttaattc
    11401 tggataaaga gcaggggttt caatcgattg acccatacgt cgtcttgctg cctgcaaatc
    11461 cgtcgttcca aataattcga tcgaaccttc atcaatcgag atcaaaccca taatagttct
    11521 cataaaggta gacttacccg ctccattttc cccaatcaat ccatagatca ttccacgttt
    11581 gatttcgatt gatactttat ctaatgcttt ggcagcaccg tattttttgg ttataccagt
    11641 tgctttcaag accgtttcat ttccaaaatc catacttatc cttctttctt attgatgagt
    11701 gaagtatagt tgacgggtat aaagaaagca gccagaaatg tataaagaaa gtgtaaattt
    11761 tccaattgaa tgtcaaaaaa aacaatcgga caatacgtgt ctttcctatt atcaggcagt
    11821 ttgttatacg cttgtatcag tttcctgatt ctttcttttc gaccaaagtc gtttctctgt
    11881 attcctcttc actgaaaatt tttgcttctt tcagatcaag cttgagtaat tttaccagac
    11941 cggttataag gataactata gcaagactcc ctcccagaaa aattagactg tctcgcagac
    12001 gtggttgcgt tgtcttccaa tcaaagagcg caccaaacag tgctaatcca agtggactgg
    12061 ccaccatggt aatcgttgtt atcgttgaca taagtcgtcc ttgtatatgc gttggaactt
    12121 ctcgctgaag ccagaccata taaaaagcaa aagaaaacat taatccttaa aaacggactt
    12181 atgaaaaata atacatgccg tttgggattt acaattagct accaaataag aggttatgac
    12241 aaaagacttt tcacaatccc ttcttccgaa taaacggtgt tctcctgcca actcctcggt
    12301 cacaagtcac aatatttatc aaacatgaga agcaattttt gtaaattcct tcttatttct
    12361 tgaagctgac cacttctgtc acaacctcag aagcggataa ataatattgt ttgatgatac
    12421 tttatctttt tcccgtttgt ttatagatcg aatgatatta tagaccaaga atcctaccta
    12481 ttaacatagc taaaaacaca aaaaaggcac tttccaaatt gtggaaagtg ccagaaaagt
    12541 taggattaag gtaactgcgc tcagcattgc ttcgcccagt acgattctcg taactcgtga
    12601 actcgaacga taatcgcaat taacgttttg agaactgtga agctttacgg gctttcttaa
    12661 gacctggttt tttcttcgta tcattgaata agtttgaaaa accttgattt ataaaggttt
    12721 tcccgtaaat atattttatt atatttgatt aaatttgaaa tgtttgtgtt ctaaaatgtg
    12781 ttctaattta aaggcagtat atttcgtatt ttagattaaa tttttaggct cattcattct
    12841 caaatccatt ctattcagtc aataaaaatt ttaaaaagac tttataaaca aagatgttca
    12901 agcgagcatc ttattttttt gtgcttaatt tgaaaggaga acatttcatg tataaaaaac
    12961 aaaaaaagaa attatgtgta ttaactattt tgagtttggc tgttcaagca attgttggag
    13021 caattgctcc tacgattact tttgctgatg aaattaccca tccacaaacg gtaaccgtag
    13081 aactagattt agcacatcaa tatgctgttg aaggaacttt tagtgatggt cgtccaatgt
    13141 cagaagtcac tgttccgcat tatgcagtgt acaacggctt taaacaagat attttctgta
    13201 ttgaaccagg agtcccgatt tacaatgagt tcactcctgg ctatgagaaa aacccgttac
    13261 cagatatgtc cgataaagcc aaattagttt ccgttttatg gaaaaaagta ggtacagatg
    13321 cagatactca tattgtggca cagaagatga tctggcaaga agttaatggg tatacccttc
    13381 actcaataaa acgatcagat ggtagtgccg taaatatcgc agccattgag gcaaaaataa
    13441 atcaagccat tgacgattat cagaagaaac caaccttcca taatagtacg gctaaaacag
    13501 tattagggca atcgactact gtgacggata cgaataatct gaatttgtca gagttcgatg
    13561 aggttgtgga aaatgccgca aacattgatt atcgtgtgaa tggtaatcag ttgataatca
    13621 ccccaaatgc caattcaaaa gaaagtggtg tgctgacgct taagaaatct gctggtaccg
    13681 gaacacctgt cgcctataaa aaggctggcc aacagacttt aatggccggg gcgattgata
    13741 aacctaatac ctacacaatc aaaattgatg tggaaacgga gggttcttta aagatcaaaa
    13801 aagtcgataa agaatcgggt gatgttgtac caggaacggt tttccattta gactttggaa
    13861 aaactttacc tgcaaaagac gtgactactg acaaagaagg cattgctaca ttggatgaga
    13921 ttcctcatgg tacaaaagta accattaccg aaaaatcagt accggcgcct tatacgattg
    13981 ataccacccc catgactacc accattaaag caggcgaaac catttacgtg acttcaaaaa
    14041 acgcacggga aaaagggcaa atcattctgg ataaaagcgg agttgaaaca gggagtgatc
    14101 tttggaatga caattattcc ttagctggta atacgtttgc cattcgcaaa gacagtccaa
    14161 ccggtgaaat cgttcaagaa atgaccacgg atgaaaacgg tcatgcggaa acaccaaaag
    14221 agattgctaa tgcgttggaa ttgggaacct attatgtgac cgaaactaaa gctagtcatg
    14281 ggttcgtgaa tacgttcaaa cctgtaaaag tcgaattgaa gtatgccaat caaaccgtag
    14341 cccctgttac cagtaacgta aaagggcaaa atcaagaagt cactggcgaa accactttga
    14401 cgaaagaaga caaagatact ggggataaag cacaaggtaa ggcagtcttt gagggtgctg
    14461 aatacacctt gtttactgct aaggatggaa aagctgttaa atggagtgag gcttttaaac
    14521 cagagttagc gaagggaacg aaaggttctg atgaaacggt cactttgact ttagacgaaa
    14581 agaaccaagt tgccgttaaa catttagcta tcaacgagta ttattgccaa gaaacaaaag
    14641 cgcccgaagg atatagcctt gatgaaacaa agtatcctgt atccatcaaa aaggtggatg
    14701 atagcgaaaa aaatgccgtg attacccgaa atgttacggc aaaagaacaa gtgattcgct
    14761 ttggctttga tttctttaaa tttgctggat cagctgcggg cactaccgaa actggcttca
    14821 atgatctgac ctttaaagtg tcgccattgg aagggaccaa tgaaatcaca ggtgccgaag
    14881 atgaagcgac cacagcttat aacgagcaat tagggtttga tggttatggc aagtttgaaa
    14941 atcttcctta tggggattat ttacttgaag aagtagaagc cccagaaggt tttcaaaaga
    15001 ttacgccatt agaaatccgt tctactttta aggaaaacaa agaagacttt gtgaagagtg
    15061 agtatgtctt taccattacc gaacaagacc aaaaacaacc aattaaaacg gtgaccgttc
    15121 cttacgagaa gctgacgaac aaagcatttt ctgttagttt gaaccgttta atgctctatg
    15181 atttgcctga ggaagaagat agtttaactt ctcttgcgac ttggaaagac ggaaacaaag
    15241 aattaacttc tctggattct accgagctag ttgataaatt gagttacaat ctacatgaaa
    15301 tcaaagaaga ctggtatgtg gtagcgcaag ccattgatgt ggatgcgaca aaagctgccc
    15361 aagaaaaaga tgaaaaagcc aaaccagtgg tgattgcaga aacaagcgca acgttggcga
    15421 acaaagagaa aacaggaact tggaaaattc agcataaatt aaccgccgaa caagttttaa
    15481 ataaaactat tgtcttattc aactatgtgt atgaaaataa ggaagctttt gaagcaggcg
    15541 ataagccagt agcgaaggat gttagcttga ataaccaagc ccaaactgtc agttgtacag
    15601 tggaacacca tgtttctatc caaacaaagg cccacctaga aaacggttct cagaccttta
    15661 ctcatggtga tgtggtggat atgtttgatg atgtgtcgat tactcatgat gtactggatg
    15721 gctcaaaaga agctttcgaa acaattctgt atgctttatt accggatggt acaaacaaag
    15781 aaatttggaa atctggcaag attgattatg aagtaaatga taaagaattc accaaaactg
    15841 tgcttgcgaa aaaagtagat accggaaaat atccagaagg aaccaagttt acttttgcgg
    15901 aaatcaatta cgataaagat gggaatatca atggaaaaca caatgaagat ttgaaagaaa
    15961 aatctcaaac cttaacacca aaagaagttc caaccatact aagtacgccg aaacaaccgg
    16021 aaacaccaac tgttccaagt ggctctcaag aatctagtcc cacagtgaag accttccctc
    16081 aaaccgggga gaaaaattcc aatgttttac tgttcattgg ttttactttg atttttgcga
    16141 cggcgggcta ttatttctgg aatcgccgga actaaggtga tgagatgata caagtaaatc
    16201 ctaagaacca acgtgcgcga ccgcctccta aaaaagtagt cgcgctattt ttgtatccaa
    16261 aactaacaat gaaaagagga aaagaagatg gaattgaaat ttgtcgtgcc agatatggct
    16321 gaaacctttg gaaagttaag ttatgccggt gaaggagaaa ttataacaga aggatatgga
    16381 cggaatacta cggtgattgg tcgcagttat catttgtatt ccagtaagca acgggccgat
    16441 gatattgagg tggtagtagc tgcggaagcc ggtgaaaagg attttgatca agaccaaccg
    16501 ctaaaagccg tgaatcccca tttggttgcc aaaggctatg agattgagaa tcgtgggttt
    16561 actgattacg tgttgtatgt tgatgattta gtgaaagcat aggaggaaaa gaagatgaga
    16621 ttaacagaag gtattgtcgt agattcaggt ttaacatttg ggaagttgcg tttttccgca
    16681 ttacgccgag aagtacggaa gcaaaatgag gatggaacaa ttggtaatga ggtaaaagaa
    16741 cgaacctata atttaaaatc ctctgcacaa gggcgaatga ttcaagttag tatcccagct
    16801 aatgtaccct tacgcgagtt tgcttatgat gcagaggtag agttggtcaa tccaatcgtg
    16861 gataccgttg ccaattatgt ttttcgagaa ggaactaccg ttaattggtt cattaaggcg
    16921 gatgatttag tcttgaagcg gcagccgaat caaggaaatc ctacgaacca aaatgaaaga
    16981 aagaaatagg tgaagattat ggaaattaat gttgaacata ttttagattg tctcgatcaa
    17041 tatggaaaag gcgagctgac tgaggaacaa ctcgttaaag cattaacctc tgatgaaaaa
    17101 atgtttctga ttatacatca aggtttactt ggagaaaata gcgacagtga agaagactct
    17161 gatgtactag actggttagg agaagaagag tctttcttta tagttgtcga agtaaatgaa
    17221 aacttatgtc ggcaggcaga atccattttg gaagaaattg gtgtggagat gccggatgct
    17281 attgaaggct ttttaaatca attggttgaa acgaaacaac tcccctcctc gtagcagtaa
    17341 atgactagca taactttttg aaattgccta atgaaatcct attgggcttt ttcgattgga
    17401 ggtggaaacc atcaaaatgt acaaaggaca tcgcatacgc gcaggagatc agcacttggt
    17461 ttatcacttt gttcttggct ggctactcgc tctgttcatc ggttggatga gcgtctttta
    17521 ttttcaagag tttagacaat ttgatatttc taagctatca ctttctacta ttgaaatcgt
    17581 ttggtctata aaagatttag tatgtctgct tggaagtctc gctttttcag gagctatgat
    17641 attgctttat attcactttt ttcttgatca ttggcgaagc ttgtggcatc gacaaaagct
    17701 agctcggatg attctagaga accattggta tgaagtgaaa caaactcaaa gtgaaggctt
    17761 tttcaaagat ttgaatagta gccgaaccag agagactatc agttacttcc ccaaaatctg
    17821 ttatcgaatg aaagatggat tactgtctat tcgcgttcaa atttcacttg ggaaatacca
    17881 agatcagctt ttaaaattgg aaaagaagtt agaaagcgga ctgtattgtg agttagtaga
    17941 aaaggaactc aaagactcct atgtggagta tactttactg tatgatatga tcgccaatcg
    18001 aattgggata gacgaagtag tggcagaaaa tgggactctg cgattgatga aaaatcaagt
    18061 atgggcttat gattctttac cccatatgtt aatcgctggt ggcacgggtg gagggaagac
    18121 ctatttcctc ctcaccatta tcgaagcact attgaagtca gatgcagaat tgtttatcct
    18181 cgatcccaaa aatgcagatt tagctgattt aggtacggtg atgcctcatg tttattcaca
    18241 aaaggaagaa atttctgctt gtgtggaaga tttctatgaa cgtatgatgg ctcgtagcaa
    18301 ggcaatgaaa gaaatgtcta actacaaaac aggagagaat tatgcctatc ttggacttcc
    18361 acccaacttt ttaatctttg atgaatacgt ggcttatatg gagatgttaa cgacgaaaga
    18421 aagtgcggtg attttgaata agctcaaaca aatcgtgatg ttgggtcgtc aatctggttt
    18481 ctttctgatt ttggcttgcc aaaggccgga tgcgaaatat ttaggagacg gaatccgcga
    18541 tcaattcaac tttcgcgtgg ctttgggtcg tatgagtgaa ctcggatatt ccatgatgtt
    18601 tggggaggtc gaaaaaaact tctttatgaa gcacatcaaa ggtcgaggtt atgtggatac
    18661 aggtggcagt gtgattagtg agttttatac gccacttgta ccaaaagggt atgatttctt
    18721 agattcaatt agtaaattag aaaagatgaa tgattgagtg atcttcaata ctgactttat
    18781 acttcaagta acgtataata cttttatata gtaggaagaa aggggataat taatatggaa
    18841 agtaatgatt cttatttaga gtatgcaaaa gcaaataagg ataagtttat ttcttatatt
    18901 atcaaaggga aatcccccga agttggacga gacgcagttt ttatggcagg gagtcctggg
    18961 gctggaaaat cagaggttgc cattggatta gctgagaatt atgataacca tgtgattatt
    19021 gatgcggatt actttcgcac ccagtttcct gattataatg gaaaaaactc tagtctcttt
    19081 caaaaagcat cttcttggtt ggtagagcag gcatttagat atgtcttaga tcatggctat
    19141 tcttttattc tggatgcaac ttttgcaata ttaagtgctg aaaaaaatat ctcgcgggcg
    19201 ttgaaaaaac attttcgagt gacgattttc tatgtttatc aagaccctaa aatcgcttgg
    19261 aaatttacac aagaacgtga aattgcagag ggacgtcatg tgccgaaaaa ggcatttatt
    19321 aacgcttttt tcaaatcaag agagaacatt gcaaaagtaa agagtaaaca tccagatgta
    19381 ttgcttcata tcataataaa agattatacg aataatattt ccgaagttca ttatgctacg
    19441 gataatctag aattattttt accactaggg tatagtagga aagatttgga ggttcaatta
    19501 catgactgag aaagaacaag taacgaaaat tgtaaagaaa tacaataaga gcattgcgga
    19561 tttatcggaa aatgctactg caaaagagtt caaaacggtg attaaatatg tagccgatca
    19621 ggccaatgaa aaacaacgga aattagttgg attaaataaa aaatagtagg acaaatt
//
LOCUS       DACECR010000051        19539 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.051, whole genome shotgun sequence.
ACCESSION   DACECR010000051 DACECR010000000
VERSION     DACECR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.051"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            108..338
                     /locus_tag="IWO17_001669"
     CDS             108..338
                     /locus_tag="IWO17_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290550.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            523..1068
                     /locus_tag="IWO17_001670"
     CDS             523..1068
                     /locus_tag="IWO17_001670"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0290551.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            1069..1386
                     /locus_tag="IWO17_001671"
     CDS             1069..1386
                     /locus_tag="IWO17_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0290552.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(1543..1707)
                     /locus_tag="IWO17_001672"
     CDS             complement(1543..1707)
                     /locus_tag="IWO17_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HAQ0290553.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1809..2330
                     /locus_tag="IWO17_001673"
     CDS             1809..2330
                     /locus_tag="IWO17_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAQ0290554.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            2302..2757
                     /locus_tag="IWO17_001674"
                     /pseudo
     CDS             2302..2757
                     /locus_tag="IWO17_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(2797..2955)
                     /locus_tag="IWO17_001675"
     CDS             complement(2797..2955)
                     /locus_tag="IWO17_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HAQ0290555.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            3048..3809
                     /locus_tag="IWO17_001676"
     CDS             3048..3809
                     /locus_tag="IWO17_001676"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0290556.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3996..5348
                     /locus_tag="IWO17_001677"
                     /pseudo
     CDS             3996..5348
                     /locus_tag="IWO17_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            5364..6584
                     /locus_tag="IWO17_001678"
     CDS             5364..6584
                     /locus_tag="IWO17_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0290557.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            6619..7296
                     /locus_tag="IWO17_001679"
     CDS             6619..7296
                     /locus_tag="IWO17_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ0290558.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            7293..8486
                     /locus_tag="IWO17_001680"
     CDS             7293..8486
                     /locus_tag="IWO17_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0290559.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            8501..8827
                     /locus_tag="IWO17_001681"
     CDS             8501..8827
                     /locus_tag="IWO17_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ0290560.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            8831..9166
                     /locus_tag="IWO17_001682"
     CDS             8831..9166
                     /locus_tag="IWO17_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0290561.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            9163..9558
                     /locus_tag="IWO17_001683"
     CDS             9163..9558
                     /locus_tag="IWO17_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290562.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9555..9926
                     /locus_tag="IWO17_001684"
     CDS             9555..9926
                     /locus_tag="IWO17_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290563.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9942..10502
                     /locus_tag="IWO17_001685"
     CDS             9942..10502
                     /locus_tag="IWO17_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0290564.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            10627..10938
                     /locus_tag="IWO17_001686"
     CDS             10627..10938
                     /locus_tag="IWO17_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290565.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            10962..11153
                     /locus_tag="IWO17_001687"
     CDS             10962..11153
                     /locus_tag="IWO17_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290566.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            11153..15009
                     /locus_tag="IWO17_001688"
                     /pseudo
     CDS             11153..15009
                     /locus_tag="IWO17_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            15010..15882
                     /locus_tag="IWO17_001689"
     CDS             15010..15882
                     /locus_tag="IWO17_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290567.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15879..16928
                     /locus_tag="IWO17_001690"
     CDS             15879..16928
                     /locus_tag="IWO17_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290568.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16932..17756
                     /locus_tag="IWO17_001691"
     CDS             16932..17756
                     /locus_tag="IWO17_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290569.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            18314..19539
                     /locus_tag="IWO17_001692"
                     /pseudo
     CDS             18314..19539
                     /locus_tag="IWO17_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331001.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6772 a         3347 c         4368 g         5052 t
ORIGIN      
        1 ggtaacggtg agatagtacg aaagtataga agaccaataa agagcgatac atataaacga
       61 aaggaaaaca atgaagttat ttcttttcgc agaaggagaa aagccaaatg agaaactact
      121 ggtatgtatc gctaactaat gaatatcctc gaaccattga tgattgttca gtgcgtgttg
      181 tgcgttctgt acaaatcaaa gggaagtact ctattgtcga aatgctaaga gaagctacac
      241 caaacgaagt ggataaatgc aagctgatat attgcggtca tggctattgg aaagacgagt
      301 atatccaaca aaacattgag aggtggatag atagatgagt tacctcgaac atttgaaacg
      361 ttgctacatg cattctaaga ataaacttcc tgacagctac actgtagatg atgttgctat
      421 acatgtattg aagactgaga gccatagcag tcctgatggt agcagtaagg agcagacgct
      481 cgcgtggttc aagttcttta agtggattaa ggaggaagag taatgcacat gcacatagga
      541 gaaaagatat ataaattgag acggcttaat aacgaaacaa tgaacgactt agttaaacaa
      601 actggagtgt caaaatcatg gttatctgat attgagtctg gaaaaaagca acgcgttgat
      661 tttaataaga tatgtgcaat agccaaacat tataaagtta gcttagaata ctttcgggat
      721 gattatttgg atagtgatga acacgaggat attattcctt tgacaggcga aacaagagtt
      781 aaatcagtga aatcgcttga acaaagccta aagtatctga ataacaaaca agaacctaac
      841 cttctagaga tcaaactaaa agatactgac tcagtaccag aggtttggta caagggtgag
      901 aggttggatg aattgcctaa aggattagta gatgtctcgt atcattggaa gactgatgat
      961 tttactaatg atgatagagg agcgaacgac atcacgattc aatacttttc tagctttaat
     1021 gataagtatc cagataagaa aacaatcgga cacaagagag atatgtaaat gaaagaagtt
     1081 agacctagag acgaaataga caaactatac aagaccaaac gatggcgaga cctaaggcaa
     1141 gtagtaatag ctagggactt cggcatgtgc caagagtgca agcgtcgagg gcggaacaca
     1201 aggggcacga tcatccatca catagtcgag gcgagggaag acctgtcact gttctggtcc
     1261 gtagataacc ttgaatgtat ctgtgtagct tgtcacaaca gagagcatcc agagagatca
     1321 ggcgggaaga agaaaccaaa acctaaatca catatcgtta aaatgtattc aactcctgaa
     1381 agataagttt gcagcgaaat gaaggtagcc cccctactct aaaagattaa agagtaaggc
     1441 ttgagaagaa cggtgctgtc cttccttcgt aaaaagaccg cttttcaagt tttttggaga
     1501 aaaaggaaaa agccgaccaa tttaagccgg ctttggacga agctatttct tagtccattt
     1561 gtttcctttt tgagaagtag ggggtaatcg gtcgcctgga tcaattttta cttcttgtcc
     1621 gccttggaca tttccaccac gaggacccac ttctttgtag gttccttttg gtttgttgtc
     1681 ttcgccgggt ttatagagtt ctcccatagg aatccctcct taacaaattt cggcacagca
     1741 ctgccgataa cctaattata aggattgtga taacgatttc aatctatctt ttgaaagaag
     1801 gtgatattat gccgcaacca gcgaagagtg caaaattaca attattaaac ggaaacccaa
     1861 ataagaagaa taccgaagaa ctccgcaagc gagcggccgc agaagacaaa ttaaaaatgg
     1921 ctactgacaa aatcaaaccg ccgttatggc tagattcgct aggaaaggat acctttgagt
     1981 ttatcgccga tgaattgctg tctgtggatt taatcagtaa tccggacgtc catacaatgg
     2041 ctctatactc caattggtat tcgcaatacg tttctttaga aaaacagctt cgaaaactac
     2101 aacgagagta caagttgaac tatgcgcttg cgaaaaagga ggcagaggcg agaggtgagc
     2161 cgtttaatga acctaatgaa ttaattggta atccgctctc tcggcagatg gatacagcgt
     2221 ctcggaatct ccgttctttt ggcgctgatt taggactatc accagcagcc agagctaagt
     2281 tagctattaa gatggctgat gatggtggtg atgacgatga cgacttctaa tattttggat
     2341 atgtcttaca cagaacgtgt ggactattgg caaagctatc ttgaggagca agcttcttgg
     2401 ggtggctttt taaaatgtcc atatccggaa ttgttaacta cttggtatgc ggaacgatta
     2461 atcgatggaa gcataccagc cagcaaagaa aatattcaag ctgctaaacg gcatatgcgt
     2521 gatttgcagc gccaaggaac agatgatttt ccttggatct ttgacgaaga aaaaggtcac
     2581 cggcctatta gatatatcga aaaaaaatgt aaaccaactg aaggcgactt tggttcgttt
     2641 gttttgcaac cttggcagca tttcataatt ggatccatgt acggatgggt acatcgtgat
     2701 acaggagagc gtcgcttccg cgaggctctt atttttgttg gacgtaaaaa cggtaagttt
     2761 tgccgtttaa aaatcgggca aaatcggtga aaacctttat ttttggcttt cttttagtaa
     2821 taattcgata gctttatcaa gcaatttaga cataggaacc atagtttctt ccgacatttt
     2881 ttttaatcct tcgtataaat ctttatcaat tgcgttagaa attctaattc tgttttttag
     2941 tccgtatttg tccatgtgtg acactccttt ttattttact ataacatccg attccaccgc
     3001 ttgcaactac catcaattga tggtagtata taaaagaggt gagtagattg aaaaaataca
     3061 ttatttacaa acacttaaat aaaataaatg gcaaaattta tatcggtgtc acaaatgata
     3121 ttggcagaag gtggaggagc ggtgggattg aatataaacc accaaagaac gaaacccaac
     3181 accacagaag tttttggaat gcaatacaaa aatacgggtg ggaaaatttt gatcatttga
     3241 ttattgaaga agacttaacg atgaaagaag cttttgagaa agaaaagttt tatattgaac
     3301 tttatgactc aactaataag aaaaaaggtt ataacatcgc aaaaggcgga aatggcggaa
     3361 taatatataa agttcacccc aaaggaatgc tagggaaaaa acaatctaaa gaattttctt
     3421 caaatcacag taaatgggca aagaaccata agaataattg tatgacaaac ggtgatgttg
     3481 tatggggtgt tactcacgaa cacccaaaag gcatgttggg aaagcaccac agcaaagaat
     3541 caattatgaa aaagaaagca tatagtggtg aaaatgctgt aacctcaaaa ccaattgtcg
     3601 caattgaacg ggatggaact aaaagagaat ttcacagcgc taagctttgc atggcttact
     3661 attcgattag tacatgtgtt ttttaccgac ttttaaaaga tggcgcccct tatgtgataa
     3721 atcctaaagc gaactacaga aataaagaaa aaatattagc tatcgaagga attatgttca
     3781 aacataaaga agataccgag gtaagttagt agattgcgaa aggttactaa ctaccgtaac
     3841 gcgtaggagt tgaataaata taatgctccc aagagtgtcc gacagcagat aatttgctga
     3901 aaatgtacgc taaactgggc cagaactgac tgaccgatga aaatgagggt gacctccaga
     3961 gtgcgagata aaaagctcgc agataataac aatcgaaaac atcactaatt tcaggccttt
     4021 ccacatacat ggtcgcttat gatgatgaac aaggcgccaa cgtttacgta ttggcaaatg
     4081 ctcgtgatca agcaagcttg ttgtttgata aggccgcaga aatggtcaaa caatcgccgg
     4141 cgctctttaa gaaatttggt aagcctaaac gatcaagtat taattatgct cccgcctttt
     4201 ctaaaatgga accacgcgcc tcagatagcc ggaaattgga tgggctaaac actcactttg
     4261 gtatttttga cgagatccac gagtttacga attacaatct gatcaacgtt atcaagaaat
     4321 caagaggaac cagaaaacag cctctgatag tttatatcac aactgctgga tatgtattag
     4381 atggtccgtt gatgtcttat tttgagcaag gtgtggactg tttggaacat ttggaagatg
     4441 acatcgatga acggactttc tattatctgg caaaacttga cagtgcggaa gaggctgatg
     4501 acccaagatt atggatcaaa gccaatccga atatttgtct aatgaatttt gttggcatgc
     4561 tagatgacta tgttaaggat aaaaaagatc caaaagaata tgctgactgg attaccaagc
     4621 aatttaactt gttttccgat atcgatgagc tgtcatttgt cgatatgcct accattaaac
     4681 gaaacaataa aaccatcgat attgaaacgc tcaaaggtaa gaagtgtgtc ggtggttttg
     4741 acttgtccga aacggaagac tttaccgcag ccgttttaga atttccgctt gaaacaggcg
     4801 aggtattcat tttgcaacac acatggatcc cacaagctag atttgatcga gataacaatc
     4861 aagagcgtat caaagcgtgg gagaaggtgg gagatctaac gattattcct ggtgattacg
     4921 tcaattatga atacgtctta aattggtttg tagaaaattc gaaaatctat gacattgtaa
     4981 aaatcaatta tgacaaggcc aaggcgctac gattaaataa agaactagaa aatgcaggat
     5041 ttgaaaccaa cgagattcgg caagggtttc tatcattagg tgggccaatg caaaacttca
     5101 aggaaatgct attggacggt aaggtgattt tcaacaattc caagctttac cgatggtatc
     5161 tatccaacgt caagctggtg atggatcgca actcaaactg gatgccgtct aagcagtcca
     5221 agagtagaaa aatagatggt tttgcagcaa gtttgaacag ccacgccgaa gtgttgaata
     5281 tgttggttaa tcctgtcgga accgggaaag taacctatta ctcgatttcc gatttaatga
     5341 atatgtaaga aaggtgtgga ggaatgagta ttttagatcg tttgcgttct tttggccgag
     5401 cgaagccgaa agcgagcaaa caagagtatt ttttgaatga cccgggattg ataccgtatt
     5461 tagtcggaaa agatgaaata tcagaaggga ttttttccgt aattagccgt gtatcgaacg
     5521 tttttgcgtc tctccctctc aaaatgatag atgtggagtt tggccaaccg gacgactgtc
     5581 ctgcatacaa cttgttgagc gaaggccctc gatattttac aaagtttgat tttttccggg
     5641 acgtggaagt tttgagaaac taccaaggga atgcgtatgt gcagattttc cgaaatatca
     5701 atggagaagt agcagatatg gcgttagtaa aacctggtgc ttgccatcca gtgattgata
     5761 tggatagcgg ggagctttac taccaagtaa ctgcgactga caaaggcagt tacaagcaag
     5821 ttatctatgt acattacatg gaaatgcttc actttaaaca accgaggttt ggcggcttgg
     5881 aaggtacaga ccccacaaaa gtattaacga ataccctcgg atatgatcga gaagtccgaa
     5941 aaatctcttt aagtcagctt aaaggaagta atgaagggct aaaagttaag tttgctagca
     6001 atatggatga agaagctaaa aaagctacag ttaaaaacat tgctgatttt tatcgacaaa
     6061 acggtggact acttgtggaa gaaaacggtg tagaaatcga acgtttacaa cgagagctgg
     6121 tagacagcaa gcttttagat actgataaag tatctcgctc cagaatcgct atggtctaca
     6181 atgtgccgga gcatttcatc gggaataacc agtcgagtta ctcctctcag gaacagctca
     6241 atatggaatt tttgacgtac aatctagtac cgaccgttaa tcaatatgaa gcggaactaa
     6301 ataagaaaac actatcgaga accgaaaaag caaaaggtta tcgatataag tttaatgtcg
     6361 caagtttgct aagagctgac actcaggcaa ggggacaatt ctatcaaatc atgcgacgag
     6421 gcggagcata ctcagcgaat gatgtccgaa gatttgagga cttgcaaccg ataaataaaa
     6481 ccggtatgga tgattaccat atttccggag acctatatcc aatcgatatg gatccaacat
     6541 taagaaaaac aacctcgtct aaaagcgtag ccgaaaacgg ttaggttttt ttagtttgca
     6601 ccgaagggag gtggaaaaat gaaaaaagtg acgttaagcg gcgatgtcgt ggataacgat
     6661 accgcgtggc tttatgactg gtttgggatc aattgtatct caccagggaa aatttctgcc
     6721 gctcttacag aagcagcggg ggatgaagta gaacttgata tctcatcgaa cggtggggat
     6781 gtcctagcgg caagcgaaat atataccgct atccgtgcct atccgggaaa ggtatccgga
     6841 aatgttgtga gtattgcagc aagcgctgca agtgtaatcg cttgcgcttg cgaaccgctt
     6901 agaatctcac ctacggcaca catcatgatt cataacgcat gggtgaccac tagtggcaac
     6961 gctgaggaat taaaagccaa tgcagaaatg ttaagcagtg tggatgagtc tattgttaat
     7021 gcttacgaga tcaaaacagg actcgatcgg aaaaaacttg ctgatttaat ggcgaaagat
     7081 acttggttaa atgctcaaac agcagtagca gaagggtttg cagatgaaat tatgtttgca
     7141 gaagcaccag taacggtact caatgcctct caaccggtta ttccaaaaaa cgcagtaact
     7201 aagttgaaaa atttaatact caaagcggaa gcaccgcaag aagaaacact cttacagaaa
     7261 aaactaaaag ccttaaatgg agggaaaaac gaatgaattt agaacaatta aaaaatgcgt
     7321 gggtcgaggc gggaagtaaa gtctctgact taaatgcaca actcaatgca gcattggttg
     7381 acgatgaaaa aacagaagaa gatgtagtaa gtttgcaagc acaagtaaaa gcagcacggg
     7441 ctaaacggga cggattgaaa gagcaagtgg caaatatgga agccgaacaa gtcttaaacg
     7501 tcaaaaaaga accattagat aaaaaagatg aaaacttgaa aaacaagttt atcaaagact
     7561 ttaaagcgat ggtcaatggt gatcctgcta ttatggctac tttgacatct aatacggatg
     7621 aatctggtaa tgctatcgga ttgactattc ctgtagatgt gcaaacgact attcatactt
     7681 tggttcgtcg gtttgactct ttgcaagaat acgtaaacgt tgaaaaagtg accactacca
     7741 gcggttctcg cgtttatgaa aaatggtctg atattacacc gttgactgct ttggatactg
     7801 aagacggtga aatcccagca aatgatgatc ctgcacttta cttgatcaaa tacttgatca
     7861 aacgctatgc aggtatttcc acagtaacta acagcttgct aaaagatact gccgaaaaca
     7921 ttttggcatg gttgtctaaa tggatcgcga aaaaagtagt tgttactcgc aatacaaaaa
     7981 tcttggcagc tattgatgga atcaaagcgg cacaaaagaa agatgttaca gatgttgatg
     8041 gaattaaaga tatcgtaaac gtccaacttg atccagctat cgaagctaca tctatgttta
     8101 ttacaaacca agatggcttc aatgttttag ataaagtgaa acgtgctgat ggatcttact
     8161 tgttacaaaa agacgtagct tctgcaactg gatatacctt cttaggtaaa ccaatcaaga
     8221 aaattgcttc tcgtttcttg ccaaacaaag gtacgcaagc tactcctaaa tatccactgt
     8281 acattggtga tctgaaagaa gccgttacat tgtatgatcg cgaaaacatg agcttgctga
     8341 caacgaatat tggtggtgga gcttttgaaa cagacaccac taaagtacgc gtcatcgatc
     8401 gcttcgatgt gcaactagtt gatgatgaag cggttgtttt ggctactttt acaactattg
     8461 cgaacgagac accggcgaaa gtttaaggag ctgattcctt atgattcttg atcctaaaat
     8521 ggatttaggc gaaatcaaaa acgcactaaa gattgatacc gatgatgacg atgtggaagt
     8581 aagccgtgcg gcacaagctg caattgcata cattagaggg gctatcggga atgataagcc
     8641 ctctttttat aagcaagaaa atgaaacggt tgatctgatt aatttagcta tcctgcaatt
     8701 agcagatcat tattacaacg ctcgatcagc aaccgtgagt gggaacttgc gagagtacga
     8761 tttaggtttt acaagcctaa tcttgcaact caaagcaagt tatttgcttt ttgtggagga
     8821 ggagtagcgt atgcccctta tccaaacagg aaatttaaat caacgcatca agtttgtccg
     8881 agatacgacg gttaaggatg aggacgggca agttgtcccg acttctacaa ccattcttac
     8941 ttgctgggca agtgtgcaga cacaacgcct gaacgatatt aagacgtcga ttggtacggc
     9001 tttggaagga acactgacgt tcattatccg ctaccaacaa aaatcagagc tgaccaatga
     9061 tatgaaagtg cgttggaatg gaaaaacgtt tgaaatcatt acgattacga aaggcgagtt
     9121 tgcgaaggac ttcacgacaa tcattgcaaa agaggtttca aaatgagtgt agaagtcgat
     9181 gcaaccgaag tgtacaaagc gcttagggaa gtaaaagcaa acgttcaacg agtggaaagc
     9241 ccagcactta gaaaagctgg ggagtacgct caagaagagt tacgacaaaa cacaccttac
     9301 tgggatggaa cgaagtcaaa cggtaaacgt ggttcgtata tgcaagaaca tgctaagaac
     9361 catgtggtta caagctcgat aaaaaacgga ttggtagaag tcggctatga caaagatgtt
     9421 tcttggcgga tgcactttat cgagttcgga acaatcaaac aacgtccaaa aggtttcgta
     9481 caaaaaacac aaaagcaaat cgaaaaacaa gtaacacaaa tcattgctga cgaagtaaaa
     9541 aggaggctag gactttgaaa acggcagtat cacaagtcta ttcaattctg aatagcaatg
     9601 aaaaaacaaa gaacattgat ttttacacca atagtgttcc ggaatcagct caaacagtac
     9661 ctagccttcc agttggcaga attacagaga tatccggcaa ctatgaagat tttgcaagca
     9721 acaatccttt gaccattcaa tttaacgtac aggtagatgt atgggtgtca accatgaaag
     9781 aggttgatgc cttttatttt gcccttgatg aggttatgag ggggaatggt tggcaatgcg
     9841 catacacgga acaaacagat gacgaggact tggaaggtgc aaagcggatt atcaaacgat
     9901 atgtagcaaa tatttcacta aattaaaagg agagaaaata gatggcaaca gtaggattcg
     9961 agagcgtcat ttttggcgta aaaacaggtg caggcggcac tctaaaagaa ttagtagcag
    10021 ataagtcgaa aggcggagcg atcgaagcta aaattactgg attaggcgca acttctaaca
    10081 caacatacgc ttcaaacgta ccgttcttca ttgcaagtaa aggggtttcg tcgccaaaag
    10141 ttacgcttga cgtggcagac ttaatggata acggcattta cagcgaaatc attggtgcta
    10201 aaaccgtgga tggtgcaaat gtaattggtt cagaaactga agcgccttac gtgtcggtag
    10261 tcatggttac agcgaacaaa gaaggaaaac gcttgttcat gggattgaca aaaggaaaat
    10321 tcagtcatcc agatatcgac atgaaaacag ctgaagacaa aggggtagaa ttgcaaaccg
    10381 attccatcga aggggaattc atttctgatg aacgtggcta tgtatactta acagccgtag
    10441 aatcagaagg aatgacctta caaaaattca aggacttggt aaataacaaa gagggggagt
    10501 agttaaccct gcatctacac caacgacaga tacagggaca ccaaaagaac cagaaccaaa
    10561 aattgataca caaggttagc catttttggc tagccttatt ttttgtaaaa acaaggagga
    10621 aaacaaatga ttgaattgca attgaaactt gacggaaaga aaaaaacatt caaacaacaa
    10681 gatatttccg cacgtgcaat gcgtgagtgt atcaaatttt acgagaaagc ggaaaaagca
    10741 gacctaactg atttagaagc gattgattca atgattgcaa ttacagcaga tattttccaa
    10801 gatccagcag ttacatttga tgctatttta gacggtttga ctgcgagcga gttagtaccg
    10861 gcattagaaa gtgtttttga acaaataaat gaactgggaa acaatgaaaa aaagcagacg
    10921 gcgagcaaaa agaaataagt ttttctgaag ctaggaaagc aatggatcaa atctacaaag
    10981 atttaatcga agcaggttgg acgatgagag atgtggacga agccgactat cattatttgt
    11041 tacacctttt tggagaagtg gagagtggcg aagaatatgt agatggtgct gatttcatca
    11101 aacaattttt atcggctgaa gacttagtaa aacttgagga aggaggtaaa taatggcagg
    11161 aaaaggacaa ccggcaggaa atatcaagct agggattagt ttagatagca ctagttttgg
    11221 taacacgctg gacgaaatca atgcgaaagt caaacaagct gagtcgaata tgcgtgccaa
    11281 tctaaaggct tatgattcag caggacgttc atacgaagca cttagtcaaa agacgaaaga
    11341 cttgtctacg gttatggaag ggcaaaacgc caaagtaaga gaattaacaa agcgccgtga
    11401 tgaagcgatt agcaagtatg gcgaggaatc gaaacaagtt gctaacctta acacacagat
    11461 aaacaatgct accgcaaaat ataatgctta cagtcgccag ttgaacgaca caaaaaaaga
    11521 attggtgtat tccaaaacag ccgtcaatga tttatctaat gaaatcaaag aaaatgaacg
    11581 acaaatgaac gccgaagtaa aagcgttgaa agccgctggt gatgaatctg gtgcttttga
    11641 agcaaaacaa aaagggctag ccaaacaaac ggaattatcc gagaaagcta tcgaagaaca
    11701 gcgcaaagtt gtgaaactga tggctgatga gtttggcgat tcagcaaatg aaaccgaaga
    11761 tgcaaaaagg gcattagaaa agttagaacg acaaagccaa atatctagca ggcaattaga
    11821 agcactcaaa agctccagcg atcaatcagg aaaagaaata gaagattttg gcgacaagtc
    11881 cacaaggtca gctaggaaac tggacggact aaaagacaaa ttaagctcgc taaaaagcgc
    11941 attttcgttt ggtgcagttg ctggattagc gcataacgct attagcagtg tagtaagtgg
    12001 cgtgcaaggt ttggttggcg aagcagtaaa cgcatcggat tcattgatga agttttccaa
    12061 aaccatggag tttgctaact ttgggaagtc acagatagaa agctcgaaaa aagaaatgaa
    12121 agactacgcc gataagacgg tttatggttt agaagaaatt ctgaacacaa ccgcacaatt
    12181 ggcatctaat gggattccta actatacaga actaaccaag gcggcaggta acttgaatgc
    12241 cgttgcaggc ggttctagtg atacattcaa atccgttgcc atgatgctaa cgcagacggc
    12301 aggagctggg aaactaacaa ctgaaaactg gaatcaatta gcagatgcga taccgggtgc
    12361 ttcaggattg ttacaagacg ctatgttgaa aaacggagct tatacaggaa acttccgtga
    12421 tgcaatggcg caaggtcaaa tcacttccga cgagttcaac caagcaattg tacagttagg
    12481 tatgaatgac ggagcagtta aggcagccac ttctacagac acattgagcg gttcttggga
    12541 gcagatgaaa tccactgtaa taaatgggct acaaagtatt atagaaaaaa taggcgttga
    12601 aaatatcact ggtttcatca acagagtaac aaaagggatt gaaaattcta ttcctaaaat
    12661 tactcaattt ataggttggt tgagagatat tggaacgtgg atcgttgaaa atagagagcc
    12721 actaacatgg attgtcggaa tcataggcgg aattacatta gcagtaaaag cattgaacgt
    12781 agcaagtatg ttgctggcaa ttactggcgg aacattggca gccccttttg tggcgattgg
    12841 tgtagcatta ggcgcactag caggtgcttt ggtgatagct tatacaaaat ctgaaacatt
    12901 tagaaatata gtcaatgcgg cttttacagc tgtgaaaaac gtagttatga gcgttatcaa
    12961 taatttggtg gaatactaca aaatgttgtg gagcgtgttg cagtggcttt gggaaaaaat
    13021 aaaagaatgg gcttcatgga ttggcaataa attcattgaa atgaagaaca gcgttgtgaa
    13081 tacggttcag aacctatgga atggcgtgaa aaacttcttc agcaatggcg ttggagatac
    13141 gtggaataag gtagtcggtt gggtaaaaaa cattttcaac aaagcaactg aattgaagaa
    13201 caacgtttct gatgtaatcg gtaacctgtg gaatggtatc aaagacacat tccgtagagg
    13261 tatcgatacg gtattcaatt ggttttcaga actaccaacg aagatgaaga atgccattat
    13321 tggcggtaaa aacgccattg ttgatgcgtt caaaagtatt ttcaacgcag cacttaaagc
    13381 gataggtaaa ccagttaacg caatcatcca tggagcttca tgggtactag aaaaattggg
    13441 cgcagaacct ctacaagaat gggatgtacc acaatacgct acaggtacac cagcaggtgg
    13501 gcacccaatc aatggtccaa tgatggttaa tgatggacgt ggagcagaaa cagttatcac
    13561 accagatggt agagccttca ttcctaaagg acgcaatgtt gttctaaacg caccaaaagg
    13621 aacgcatgtc ttgacagcag aagagaccgc gcaacttcaa ggttctaaag ctcctaagta
    13681 tcgttacaaa aaaggtacta acttctttgg taacatgtgg gatagtgtga aaaaggttgc
    13741 tggtaatgta ggtcacacaa ttaaaaacgt agtaggtgac gtgtgggact ttatttcaga
    13801 cccgggagcg ttagctagaa aagtacttgg ggggttagat gtattaggtg gattgacaaa
    13861 gtatccatta gaagtaggta aaggcatcct atctaaagca acaagtgcac tgactgaaaa
    13921 aatcactggg ttgttctcat ctggtaactt agatacctcc gtaggaacaa atggcgtcta
    13981 taaatatttg gctgatgttg ctaagtctgt gatgaagaaa ttcccaggat ttgtggcaac
    14041 tagtgggtat agacctatag accaggtgac ccttattcac atggtaaacg taatgccatt
    14101 gatattgcat taccaggcgt cacaggaggc tcacctagat acacagaagc agcaaactac
    14161 gcttttgaca aattcgcttc caaaattggt tacgtaatca ctaatgggaa agttcgtgac
    14221 cgttcaggac aatcaggtca accagcaact ggtgcatggg agccatggcc cgatggagat
    14281 cactatgatc atgtgcattt aaacggtgtg aaagacccac aaaacactca aatttcagga
    14341 gatagcgtgg gaggcagtgg ggtagaaaga tggcgcaatg tagcaattag agcgttgaaa
    14401 atgaccggtc aatacagtac tgcaaactta aatgcattac taaatcaaat gcgtacagag
    14461 tcaaatggta atcctaatgc agttaacaat tgggatatta acgccaaaaa tggaacacca
    14521 tcaaaagggt tgctccaagt gattgaccca acattcagac agtatgcaat gccaggattc
    14581 aacagcaata tttttgaccc actatctaac atcttagctt caatcagata cgcactatca
    14641 agatatggct cactaacaaa tgcctatcgt ggagttggtt acgcaaacgg tggaattgta
    14701 aaccaacatc aaattgcgga aatcgcagaa ggaaacaagc cagaaattat tattccgtta
    14761 gataaggcta aacgatcaag agcgatgcaa ttattgtcta tcgcaatgga caaactagga
    14821 gtttctccta aaacttatgg aaatgcgaca actgtttcca gtgattacga aatcctagaa
    14881 gccattgaaa aacaggcggc aacaacgaat cagctgttat cgttattgct ggcatttttc
    14941 aaaggaaata gccgaactga tagagattta gctttagata ttcagaagat cttggttagg
    15001 aggatgtaga tgcgaactgt attactgaga aatagaataa acgaagaaat tgatttgtcc
    15061 acagaagact attttgctac tgggttgagt aatatggggt ttgaagtaaa aaaagaacat
    15121 gtgggacagt gggggaattt cagagaaagc agtgagagcg ttgaaatatc tgaatttcag
    15181 tcatctgtaa tcatttctgt gcatgggttt cgagaaaaag aactgtacaa ttcacttgtg
    15241 cagttcctat cggaaggtcc gtttgaactg gaatttgctt ttgacggcga aacaatggta
    15301 agaagatgca gtctaaaatc tttaagtaag actgaaatcg atccgaaaac atcgttacta
    15361 acagacactt tagaactata tttcacatca aactggtatt ccgtaaaacg cgaaaaatta
    15421 attcagcgac cgaatgtagt gaaaacacgt ggtaaggttt ttccgtataa aagatcttat
    15481 atctacactc aaaacttgtg ggagaaaaaa ggtgtattca aattcaataa taattctgta
    15541 tacctaacga atagcaagga acggatgtcc ccgctaaaaa ttcgtgtgat tgggaagtgt
    15601 tcaaatccgt attgggaagt aatccaaaac tcacaaatca tcgcaacgga tggatacttc
    15661 atagatatga ctgaaacaca gactctagaa gtatcaagcc tttttgaaga tacaacggcg
    15721 attttaaaag atattgcagg ggtagaatct tctgtctacc aacaacagga ttatacaaaa
    15781 actaattttg ttcaggcccc aactggagaa ttcagcattg tgtttcatgt tgggggggca
    15841 gacgtagaga ttgagttata cgaggagcgt gatctgtttt gattttagca gtcacgcttt
    15901 ttcatcgtga tttaatgctt tataacgaac atttattctc taacagtttt gaatttggtg
    15961 tggacgagat caacgaagag gctagtagct ttacgatgga taagtacgtt cctgtaaaaa
    16021 caggcgattt tctactagca aaatatattc ctagtggaaa atttgcatat tttggtgtaa
    16081 ttacgtcgca ggaagacgaa aaaatcagtt gtaaaagctt acttagttta gctgatagtg
    16141 agataccgac tgcacgtgtg tcaggagata actacgaaga gcatatccgg cggttgattg
    16201 aatactattt gctgaatgat ccaacgaaac aactaaaaga tattttagac gtcaaagcag
    16261 aaagtgcgac ctctcattcg tatcaagcta ccgatacgaa taaacacaag ttaagtgcgt
    16321 atattctcaa tggtttcaaa aaatacaacg taaaatggta tttcaaggga attcaaaaca
    16381 gaaaaattta tacaggcata cgtgctgtaa atgaatcaat ttacattaaa gacaattctt
    16441 ctgaatttag cgattgggat gtgtttgttc aggcgccggg cgctggaaac gagaacaagc
    16501 tattaatcgt tgataaagca atgaaagata tagagaaacc gataatactg tcaacatggt
    16561 atttggacga agaaaacaat ttgacacaag atggatcgaa agaaaatatc acgaaaccaa
    16621 ctgtcaattt ggttaatatc tacgatcaaa ccgcagaaga taaagcatcc tacgaagatg
    16681 tggcaaaatc agagttgaaa ggcaatacgt attcgcatga aatcaaagtg aacgttgtaa
    16741 gaaatgcaaa aaatttgaat gtcgaaacga ttgaaacagg gatgtttgcc acgatttctt
    16801 ataaaggaaa gatatacaag tcggttttga cagcttggcg aatatcaagt gataaggagt
    16861 ttgtggaatt gactttcgga aacataagaa gtcgttttat ggattatttt gaagataatg
    16921 ggggataaaa aatggttagc aatgtggatg gatatcaatt tgaaaacgtg aaagtaagcg
    16981 cagaaaatga tgctagactt tatcacgttt tatataatcg gaaaaatcag gttattgatg
    17041 gttacgatca gtctatgaat ttatcttcaa gcggattaac agtaaaagtt gctgcaggag
    17101 cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga agaaagtata acagttccag
    17161 caaactcaag tggttatata gcattaacag tggatttgac acaagaagtt atacctggat
    17221 ctattcttcc agaatcggaa gaatatgaat ggactaataa tcaagtcaag ctagaattta
    17281 taacaaaagt tataaaaggt aatttaaaca atggtgataa ggtctataat ttaccactat
    17341 gctcagttaa ttctactgga tcaactgttt caatctcaaa gatatcggat agttacgagc
    17401 tgactctctc taaaggagaa attttgtgga gggggactgc gttaatgcat gatactcaaa
    17461 ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg ttttttgtta gtatggctcc
    17521 catacgaaaa cgggcaagca attgaggata gatatgtaac tacgcctttt tataaggagc
    17581 gtgtaacttt tactaatgtt ttaggagaaa ttgtttcagg atttgatgac tatcacaaaa
    17641 agtggtttag taaacgaata aactataatt cgaatactaa tatatttaca ggtgcagcaa
    17701 gcaatgcaag tggagataac gcaaatatgg tgcttaggta tatagtttct ttttagttag
    17761 gaggtatgga agagtggcaa atttagaaat taaattatct gcaaataaga gacagcctta
    17821 tctacgtcac cgtgttgttg gtagagttgg tgatgggggg ctcacaacaa tcaatgtaca
    17881 acttcttgag gaagatgaaa ttacaccttt tgtaattaat ctaaacggta ctttgaaatt
    17941 tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa ccagaaataa tcgattcgac
    18001 taatgggtta attagttaca cgttcactaa gtcaaatttc agcacgagtc atcaattcaa
    18061 acaagcatat tttgaatatg tagatcctaa cggcaaaaaa gtaacttttc agaacttcat
    18121 catagacgtg ttagaacgag tggatattaa ttcggagcaa gcgaaatact atatttcttc
    18181 attggaaaaa ttacagagtg aaatgcaaac cactttcaat cagttcatta gtgataaaca
    18241 ggtccaatac gatcaaatct actcgaaata taacgaatta gtaagattga taaatgaatc
    18301 agataagcaa gtaaatgatc gtattgatca aaccaatcag caaatcggcg atctcggcaa
    18361 gctgaaaaag atgtacagta acagcatcga ctttgggggc tatgattatt cggggaatcc
    18421 gaatttgtta agtaagctat catacgactt aattgaaaat caaaatactt cagctggaac
    18481 actttctaag ggtgaaaact cgtttaaata taataagata tcagctgaag tggaaggtgg
    18541 agtagagtta tattataaac gaagaggtat agctaactgg ttaccctcta ataaaacgct
    18601 tgtaatgact gttaagctta gagctggagc ggactatagt ccagttgacg gaaaacttat
    18661 actgattaga tataggtatg ttgacagtgg aactggcaag attgttttag acttacctat
    18721 taacagtaat tcgataactc aggaatggaa agagtttagt attactggaa ctactccaac
    18781 atttagccca caagcatacc atccttggat acaatttagg gctcaagatg gggtacttgg
    18841 ggaaatagaa atgagctatg acatcaaaat cgaagaaggc tctacagcta caccatatca
    18901 gcctaactta cttgatgact cttactggct aggtaaaaca ccgttgggtg agaatttgat
    18961 aacgaatgcc aagttcccaa ttataacacg aagcaatcct atttctggtt ttgatatttc
    19021 agaagaatta ataataggtg aaacttatac ggtatcttta aaagggacta agccagctac
    19081 taaagaattc cacttatact acggagaagc aaattaccag cagttgcagt atcaggcaac
    19141 tttacttcct gtggaaggat taacagatgt gtggagtgcc acttttacag ccagaaatac
    19201 tagtgaaacg acaaatttac ttagagtagc gttatggcaa aaacctaatt ctgcaacata
    19261 tgatactgtt caaattgact ggctcaagat tgaaaaaggc gacacacgca caccaaatat
    19321 cagtcagttt aaatactttg gtgaaggctt gaaagacagt aacaatccga acgactacag
    19381 ctgggatgtc acaccagagt atactgaaaa aagcttgaat aatacggtta gtctgactga
    19441 accgcaatcc atcgaaggat tgaaaaattt cgaagatggt attcaatcaa aaggaaaatc
    19501 tgtattgaca tcagacgaca acaaatatga agtcgtaac
//
LOCUS       DACECR010000052        19450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.052, whole genome shotgun sequence.
ACCESSION   DACECR010000052 DACECR010000000
VERSION     DACECR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.052"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            126..3653
                     /locus_tag="IWO17_001693"
     CDS             126..3653
                     /locus_tag="IWO17_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0290570.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3650..7372
                     /gene="addA"
                     /locus_tag="IWO17_001694"
     CDS             3650..7372
                     /gene="addA"
                     /locus_tag="IWO17_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ0290571.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7441..7782
                     /locus_tag="IWO17_001695"
     CDS             7441..7782
                     /locus_tag="IWO17_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0290572.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8091..9134
                     /gene="pheS"
                     /locus_tag="IWO17_001696"
     CDS             8091..9134
                     /gene="pheS"
                     /locus_tag="IWO17_001696"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0290573.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9139..11559
                     /locus_tag="IWO17_001697"
     CDS             9139..11559
                     /locus_tag="IWO17_001697"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ0290574.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="IWO17_001698"
     CDS             complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="IWO17_001698"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ0290575.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12993..13730
                     /locus_tag="IWO17_001699"
     CDS             12993..13730
                     /locus_tag="IWO17_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0290576.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13744..14559
                     /locus_tag="IWO17_001700"
     CDS             13744..14559
                     /locus_tag="IWO17_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0290577.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14572..15219
                     /locus_tag="IWO17_001701"
     CDS             14572..15219
                     /locus_tag="IWO17_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0290578.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15235..15891
                     /locus_tag="IWO17_001702"
     CDS             15235..15891
                     /locus_tag="IWO17_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0290579.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16053..16874
                     /gene="racE"
                     /locus_tag="IWO17_001703"
     CDS             16053..16874
                     /gene="racE"
                     /locus_tag="IWO17_001703"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ0290580.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16877..18232
                     /gene="rph"
                     /locus_tag="IWO17_001704"
     CDS             16877..18232
                     /gene="rph"
                     /locus_tag="IWO17_001704"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ0290581.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18233..18751
                     /locus_tag="IWO17_001705"
     CDS             18233..18751
                     /locus_tag="IWO17_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0290582.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18858..19358
                     /locus_tag="IWO17_001706"
     CDS             18858..19358
                     /locus_tag="IWO17_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0290583.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6540 a         3340 c         4177 g         5393 t
ORIGIN      
        1 gtatacttaa aaaagtagaa atatcagaac cttaaaagga tataatttga agaatataga
       61 acaaatgttc agtatttttt gatataataa aggagcaccg tatcatgtgt tgaaggagga
      121 aaagcatgtc tttacaattt attcttggaa acgggaaaaa agatcaccgt caagtcttga
      181 tcgacgaagc atccgcttgg ctcgaccagg atgcaagtaa ccaagtattt ttccttgttc
      241 ccaactacag caaattcgaa caggaacaag aaattttagc ggagatgcga agaagaagcg
      301 gaaaaaaaac tttcagcaca acaaacgttc aagtatttag tttttatcgt ctggcttggt
      361 atttgctgca acagacaact cttcttacag gaaatgaatt atcagaatca ggatctgcaa
      421 tgatcttgcg acagatttta gaaaaaaatg aggaagaatt aacaatcttt cgcggagaaa
      481 tcaataaaag agggtttatt cgtcaattac aagaactcta cagtgaattg cgtacaggaa
      541 atattcaacc agaagacctg atgcttttat ttggtgattc acctaatgca gaagaccagc
      601 aacttaaaat gaaagacttg aagctgattt tttcagctta tgacagcgaa ttgactcaac
      661 gtgcactaac aaatgaagat gcactatcta tactagcaaa ttatatgcag acacaagatt
      721 tcaacaacgt caaatttatt gtcagtggtt tttctcgaat caatggtcaa gagtatcaat
      781 tactgcaggc aatgatggaa aaggggcaat tggttgtcga tttattgctg gatcgtcctt
      841 atacagcgga tcttccagaa gtacttacgc tctttcatga gactggaaag ctttatttcc
      901 gtctttttca aagtgcaaga gaacggaaag tccctatatt agtggaccga tttgcaccag
      961 agcagactcg tccgacagag cttcaactgc tgcaaacaat gtgggaagag acatctagcc
     1021 aaaagaaagc ggcagtacac aaaatagggg gacaagagca tctccatttg tgggaagctg
     1081 aaaatccaac tgaagaagtc cgacaattag ctgttgaaat cagacgacta gtcagtgaag
     1141 aaaactaccg ttatcgtgat atccaagtgc tgacaaaaaa tatgtcttta tatggaaata
     1201 tccttcggcc gatctttcaa gaaatggcaa ttccttttta tatcgatgaa gaacaaatga
     1261 tggaaaatca tccgctgatc gaatggatca acagtctgtt cgctcttgac cgctattctt
     1321 atcggctaag tgatattatg cgttttttca aaactgaatt atttctgtcg acggaagcag
     1381 aaaccgaact tgatgaatgg aaacaagagc gagatgaatt caggaaaaaa ctggacatca
     1441 ctgaaaatgt agcacttgct tacaattatc aaggaactta ttggactcgt gaaaaagact
     1501 ggcagtttgt ggcgtatgat tttgaagcag aacagattga agacgtacag tatttggaag
     1561 aacagtcaaa tacgattaga cgattatttc agaaagctgt accagtattt ttgaaaaaaa
     1621 taaagaaaag caaaacagga caagaggccg caaatgtttt ttatcaattt ttgatccaaa
     1681 gtggcgtaca gcaacaatta ttgttttggc gtaatcagga agtagacaga ggaaacttag
     1741 aagcggcaag aaaccatgag cagacatggg gagcgctgat tgatctgttg gatgaatatg
     1801 tagtaattta tggtgaatcg gcttttgact ggtcactgtt ccaagaaatc attacaagtg
     1861 gtctagaaaa tctaagttat ggtaaaatcc ctaccgcaat cgaccaagtg cgaatcaatc
     1921 gtttggaatt agttcgacca aatcaagctg ccgtcacttt tgcattaggg ttgaatgatc
     1981 aagtgtttcc agaccgaaaa gagacaaaag gattgttgtc cagtgaagaa cgagaagtat
     2041 taaatcaggc cttgccagat ggacagtttt tattcgaccc atcaaaggaa agtatctctt
     2101 ttgagccttt ccaagcctat cttgtttttc tttccggaac agatcgatta tatcttagtt
     2161 atgcacaaag ctatgacaca gatggttcgc tgaagatgtc tccttatttg cgtagaatca
     2221 cagaatattt atctgttccc gtcgagagaa aagagcattt gacgatcgaa agcgacccta
     2281 attgttatgt agggacttac agaagcggaa tcaatacagt caataggatt tatcgattag
     2341 ctttagatga aaaaaaacct gtaccaaagc actggcaaaa gctcaaagaa ttagtactga
     2401 attcgtcatg gcgaaatttt gctttacaga tttttgaaag tcaggaacat ttaaatgttc
     2461 ctgtttcgat gccagaaaaa atggcggaag agctttatgg aaaagagatt tatacttcaa
     2521 tttcacgtat ggagaatttt tataattgtg aatacaaata cttcatgcaa tttggtttga
     2581 aattgaaaga acgaactgtt tatggtctaa ctccagcagc aactggtgat ttttatcatg
     2641 aatcattgga tcgtttcttc aagctactgt tttcaaacca attatcctta gtgaatatgt
     2701 ccgatgcgca gcgtagagac tttactgaaa aagtgctgca ggaagtattc ggagaacttc
     2761 ggtttgagat attggatagt tctgcaagaa tgaactatat ccgctaccag ttaggacaga
     2821 cgattcaaaa agtgacatgg gctttgcaaa aacagagcaa aaatacaggc atgcatcctc
     2881 ttcagacaga gattctattt ggtcagattg ctggagcaaa aggtatcaca ggtctggaat
     2941 taccgttgag taatggtgga aaactccatg ttcgagggaa aatcgatcga atcgatgtag
     3001 cttctgaaca agaagatact tggctaagcg tcgttgatta caaatctagc ggacgttcat
     3061 tcgatgtcac agaagcttat tatggcatgg ctatgcaatt attgacgtat ctggacgtag
     3121 cattgatgga tgcggtacgt ttgactggaa aggcggctgt taaaccagca ggatcatatt
     3181 atttacatgt tcataatcct gtattgacgg agacagatga tgtagagaag aatacattga
     3241 aaaaatttag ttatgatggc gtttttgtcg atgatcccgc tcttttagaa gtactagatc
     3301 attcactgga agcaaaagaa caatcactga tctatccagt gaaaaaaaat gcgaaagatg
     3361 aataccaaaa aggtagtttc agtaaagaca aatttttcac ggaagaagaa ctgcaactat
     3421 tcatggcgca taatcgggaa aatatgcggt tagctggaga tgcgattact tccggagaaa
     3481 tcaaactaaa tccagcttat aaagataaac agcggattgc ctgcgaattt tgtccattca
     3541 gaagcatctg tacgtttgat gtgatgctga aagaaaataa ttaccaccgt ctggagaaaa
     3601 tagataagga agaagcactg cgtagaatat tgaaaaggag tgaagaagag tgaatcaaat
     3661 tcctttaaaa ccgcaaaatg aacgattcac agatgatcag tggcaagcga tttttgatca
     3721 aggagataat ctattagtct ctgcgtctgc cggatcaggt aaaactacag tgcttgttcg
     3781 aagagtgatt gaaaaactaa aaatgggttt tgatatcgat gaattattga ttgtgacatt
     3841 tactgaagct gccgcaagag aaatgaaaga aagaattcaa gaggcattac aagaatccgt
     3901 caatagcgaa agtgaccctg tacgtagaca gcattttact aagcagttag ttttattgcc
     3961 cacagcgaat atctcaacct tacatgcttt ttgcctgaca gttattcgac gttattatta
     4021 cttaattgat attgatccag tctttcgcat gttaacagat gaaacagaaa cgatcttgat
     4081 gaaggaggat gtgtgggacg agctgagaga agcactttat gcagaaaatg atgaacgatt
     4141 tttccaatta acaatgaatt tttctaatga tcgttcagat gacggcttga cgaatctggt
     4201 gttttctctt tatgagtttg cccgtgccaa ccctgacccc caaaaatggc tagaacagtt
     4261 aagcgataat tatcgtttgc ctgaggggct agcgaaatct cgattatatc aaaaacaaat
     4321 ccgtccttta gttttggcag atatatacca atgtgtacag ttatatgaac agatgactca
     4381 gttagcacaa ggtgaaggat tagaaaaaat gcatgagcaa gtagctggag aacaacagca
     4441 gatcaagaat atctatgaag cgttctcgca agatcggtta gaagaagctt atgctggttt
     4501 ggaacaacta actttttcaa cttttaagag cagtcggaaa gcagaattga aagaaatttc
     4561 caatgaagtc aagggcatga gggataaagc aaaaaaactg attcagcaga taagtaaaag
     4621 ttattttcct gtttccccaa gccaaatgga agaattaacg gacaaagcac taccgttagt
     4681 cgaagaaatg acaaaagtca cgcaaagctt catggacggt ttcagtatgc gtaaacggga
     4741 aaaaggtgtg ctggatttca atgatctgga acatttagcg ttgcagattc tcacagaaaa
     4801 aacgaaagat gtatggctgc caagtgaagc ttcaaaacat tatcgaaaaa aattcaaaga
     4861 agtaatggta gatgaatatc aagacgtgaa ccaattacaa gaagccattt tatactggct
     4921 aagagaaccc gatgatacaa aaggaaatat gttcatggtt ggggatgtca aacaatccat
     4981 ctattctttc cgtttagcag atcctagttt atttatcggg aaatacgaga atttctcaaa
     5041 gaaagaaggc ggacgtcgaa tcgttttggc tgagaacttc cgctctagaa aagaagtcct
     5101 ttcttttacg aatcttattt ttgaacaact aatggatcca gcagtgggtc aaatcaatta
     5161 tgacgaagca gccaaactga tccaaggatt ttcagacttt ccagagaatg aacagtttga
     5221 gccggaaatc atgatctatg aaaaagaaca ggaagaatca gagattgaga ttccgacgga
     5281 tgatatactg gaagacaaaa cagaaggcga actttttatg acaggactaa aaattcgtca
     5341 attaattgat tcctccttta tgatttatga taaaaaatca aaaaaaagtc gtccgataga
     5401 gtacaaagac attgttctct tgacacctac aaagaagaac aacttaacga ttttagagat
     5461 tttcaaaaca ttggatattc cattagagat gaatgatgct caaaactatt ttcaagcgac
     5521 agaaattcgc acgatgatct cgctgctaca attaatcgat aatccctatc aagatattcc
     5581 gcttgcggct gtattgcgct caccgatcgt gggtctgatt gaaccagaat tagccagcat
     5641 tcgattagct aaccgtgcac atacttacta tgatgctgtg cttgcttatc aagcatcaaa
     5701 cgaagatgaa ttagcagcta agctggaaca ttttggcaag caactagagc attggcgaga
     5761 acttgctcgc cgttcttcga tcactgactt gttatgggat atctattatg agacgggata
     5821 tctagagtat gtggtcggtc tgccagcagg ggcacagaga caagccaatc tttatgcatt
     5881 ggttgatcgt gccaaagctt acgagcaaag ttctttcaga ggattatacc agttcgtgcg
     5941 tttcattgaa aaaatgcaag aaaaagataa agacttagca gaaccagtca tttcaataga
     6001 agataatgct gttcgcgtta tgacgatcca tgcaagtaaa ggcttggaat tcccagttgt
     6061 atttttgtta gatatgacaa aggagttcaa cttacaagac ttgcgcaatc ggtatgcttt
     6121 tgaagaaaaa cttggagcag gaattcgcta tatggatcct gaaacacgag tgttatatga
     6181 tacgttaccg tttcaagcca tcaaattggc aaagcaaaac aaattgttgt cggaagaaat
     6241 gcgtaaattg tatgtaggat tgactcgggc agaacaaaag cttttcattg tcggttccta
     6301 taaaaataaa gagcaaatga tccaaacgtg gtctgaagca gctgatcatg aggagttagt
     6361 ttttgatcct gcgctacgtt tgaagggcag aagcagcttg atgaattgga ttggttacgg
     6421 attgatccgt catcctgaaa tgcagaaata cttagaggaa gaaatttcga cttctcttct
     6481 tcaacattca aatgcacagt tttctatttc atggatgaat cagcaatcga tcattgaaca
     6541 aagacagttg cttgctgaaa aagaactggt taacttagat cagcagatga aagaagatga
     6601 aactttatta gcagattcgt tacaaaaacg tctggcatat gaatatcctt atcaagcttc
     6661 ttcacaaacc acaagttatc aatctgtatc tgaaatcaaa cgattattcg aagatccaga
     6721 tgatacacaa gaatcacgtt tgactctgga aagcagtcag aacaaagcag catcccgtca
     6781 atttcgttat acgcaagaac aactagcaga accaaaattt ttgcaaaaag atcggcaagt
     6841 gtcagcagct gctgttggaa ctgctacgca tgctttgtta caattactgc cattagagat
     6901 gccaactaca gagagtatcc atcaaaaatt acaggaattg gtaaggaaac ggttagtaga
     6961 cgaaaaagtc gcgaaaaaag tagatgtttc ttctattata tggttctttc aaacagaact
     7021 tggtcaacag ctgattgcca acaaagaaaa cgtaaaacga gaacaacctt tttccatgct
     7081 gcttcctgct gacgaagtat ttcaagacta tccaaatcaa gaagatgaac tattaatcca
     7141 cggaatcgtc gatggatacc tagaagaaaa agatcatctc aatatctatg attttaaaac
     7201 agattttatt ttacatccgg atgatccagc agaaatagat gcaatcgtcc aaaaatatca
     7261 aggacagcta agactttacc aacaagcaat gtcagaagct ttgaataagc cagtagaaaa
     7321 tgttttccta attttgcttc gagtgaaaca aattataaac attaacaaat aattataatt
     7381 gagcaaactt gacttatgtt ttgtaaggat tatgctatac tctgggaaga ggtgagtatg
     7441 atggagctaa cacagacaga attcgtttta tgccctaaat ttgaaaaaac attctctatt
     7501 ttaggaaaaa aatggaacgg attgattatc gatgtactac tcgaaaacgg tccgcaacgc
     7561 tttgttgagc ttgcggcaaa aatcccgatg gtcagtgatc gagtattagt cgaacgcttg
     7621 aaggaattag aaaaagaagg aatagtcact cgaaactttg cctgtagcga aaagagccgt
     7681 tcagaatatc tcttgactga aaaaggagca gacttgcaaa aagcgatgcg tcaaatacaa
     7741 gtttggagcg aaaaatggat cacagaggaa gaatgcggtt gattttgtaa aaaaaatctt
     7801 gtaaactata gtccatacga ataagtaaag aacgatgacg aaaaagagta gtttggctaa
     7861 accagttcag ggagatctgt cgcagactga aagcagatcc tgtgtttgaa aaatgaagtt
     7921 cactttccga gttcgtctga taaaatcaga ccggcaattg tcgttaacga agtaagagtg
     7981 cggtgtaagt catttatccg caaattagga tggtaacgcg aagacctcgt tccttaacag
     8041 gaatgaggtc tttttgtttt gtatttggaa tttaaaggag gaacaagcac atgtctctac
     8101 aagcacaatt agagagcttg aaagaagcaa cgctcgtcaa aatcacagcg gcacaagatt
     8161 taaatgaatt aaatcaaata cgtgttgaaa cgttaggaaa aaaaggaccg atcacagaag
     8221 tattacgtgg gatgaaagat ctaagtccag aagagcgacc aaaagtcgga agtttcgcaa
     8281 atgagatccg agatcttttg acagaagaga tcgtgcgaaa aaaacaagta ttagaagaag
     8341 aagcgttaaa tgcagctttg gcaaaagaaa caatagacgt gacactacct ggtagaaaag
     8401 taaaacatgg tacacgtcat atcgtgacac aaatcatgga agaaattgaa gatatttttg
     8461 tgggtatggg ctatcagatc gtagaaggat atgaagtaga atctgatcat tacaatttcg
     8521 aacggatgaa tctgccaaaa gatcatccag caagggacat gcaagatact ttctatattt
     8581 cagacgagat attgattcgg acacatactt caccagtcca agcacggaca atggaaaaac
     8641 atgatttctc taagggtgct ctacgaatga tctcgccagg gaaagtcttc cgcagagata
     8701 cagatgatgc gacccacagt catcagttcc atcaaattga agggctagtt gttgataaaa
     8761 acatcacaat gggcgacctt aaaggaacat tagaagtcgt aatgaaaaaa atgtttgggg
     8821 aagatcgtga aattcgtttg cgtccaagtt atttcccatt tacggaacca tcagtagaag
     8881 tagatgtcag ctgtttcaaa tgtggtggtg ccggttgtaa cgtatgtaaa tacactggat
     8941 ggatcgaaat tttaggagct ggcatggtac acccgaatgt gttgaagatg tcaggaatcg
     9001 atccagaaga atattcaggc tttgctttcg gccttggacc agaccgcgta gctatgttgc
     9061 ggtatggagt gaatgatatc cgtaatttct atcaaaatga cttacgtttc ttaagtcagt
     9121 tcaaggggga gtaagaaaat gttagtttca tataaatggc tcaatcgtta tgtagattta
     9181 tcgaatgtaa caccaaaaga attagcggat aagatgtctg tgacaggaat cgaagtagag
     9241 ggagtaacgg ttccagaaga aggcttgaaa aaaatcgttg tcggtgaagt caaggaatgc
     9301 gtaccacatc cagattctga tcacctttct atttgtcaag tcgatattgg cgaagacgaa
     9361 ctgtctcaaa tcgtttgtgg agcgcctaat gtgaaggttg gaatcaaagt gattgttgca
     9421 cttcctaact cacgtatcgc tggtaatgta aaaatcaaaa aaggaaaaat gcgtggccaa
     9481 gtatcaaatg gcatgatttg ctcattacaa gaaattggct attcagatag tgttataccg
     9541 aaagaatacg ctgaaggtat ctattatatg ccacaagaag ctgtaaatgg cgatccagtt
     9601 tttccttatt tggatatgga tgattcgatt attgaattat ctatcacacc gaatcgtgcg
     9661 gatgcattgt caatgcgtgg cgtagcttat gaagttgggg caatttatcg tcagactcct
     9721 acattcgatg acgaaaagct ggtagaatcc tcacgtcctg ctagcgataa aattcaagtg
     9781 gaagttgaag ataaagaagc agttcctgct tatcaaatca gaatcattga aggagtaaga
     9841 atccagccaa gtcctcaatg gctgcaaaat cttttgatga acgaaggcat tcgtccaatc
     9901 aacaatgtag tagatgtgac gaactatatt ttgcttttat tcggacaacc actacatgca
     9961 tttgactacg acaaattgaa atcaaaagag atctttgtcc gccgtgcaaa agaaaatgaa
    10021 acattagtaa cactagatgg agaatctcgg gaattgacaa ctgacaatct cgtcattact
    10081 aacggcaaag aaccagttgc tttagctggt gtcatgggag ggttagactc agagatcacg
    10141 aatgaaacaa caacagttgc actagaatct gctttatttg atccaatttc gattcgtcgt
    10201 acagcaaaac aatttaattt gcgtagtgaa tcctctgcac gttttgaaaa aggaatcaac
    10261 catgcgactg ttggagaagc cagtgaggtt gctgcagcaa tgatcgtttc tcttgcgggt
    10321 ggggaagttc tacaaggcgc ggtcaaaggc tcagagatca aagcagaaga cgtatcagtg
    10381 aagatttcac ttgaacgcat caatcaatat ttaggaacat ctcttgtcgt taaagaagta
    10441 aatgagattt ttgaagcact tggatttagc tatgaagaaa aggagtatgt ttattccgta
    10501 gtagtaccac caagacgctg ggatatccaa atcgaagcag atattatcga agaagttgct
    10561 cgtatttatg gatacgacca gttgccatct acacttccaa gtggagaaac ggttgctgga
    10621 agcttgtcaa aagaacaaga aactactcgt aaaattcgta caatctttga aggaagcgga
    10681 ttgagcgaag caatcagtta tgcattgaca acagaagaaa aatcaagaca gtttgttata
    10741 aatgaaagta atattacacg tttggactgg ccaatgagcg aagaacgttc tgtattacga
    10801 atgaatttga tcagcggact attagatgat atcagttata acactgcccg aaaaaataca
    10861 gagattgctt tatatgaaat tggtcgggtg ttcttccaag aaaatgatcc acgtacacat
    10921 ctgccgcaag aagtaaaaca tgcagcaatc gctatttctg gtatttggga agaaaaagat
    10981 tggcagacga aagctcaaaa tgtagacttt ttcaccatca agggtctttt agaaaactta
    11041 tttgaacaac taggcatcac tgaagatgtc cattatcaaa cagtgacaga aatgaaggaa
    11101 atgcatcctg gaagaactgc tgcgatttac cttggcgaaa aatttattgg ttttgtcggt
    11161 caagtgcatc caacgattac aaaaagttat gatattccag aaacatatgt tgctgagttt
    11221 gatttatctg ctatagtgga agctgctcaa aaagggatcg ttttcgaagc agtgacaaaa
    11281 ttcccagcag tcagccggga catcgctttg cttgtaaaag aaactgtgac aaatcaggaa
    11341 ttaacagaag ttatccgttc tgctgccgga agatttttaa cagatatcca attatttgat
    11401 gtctatcaag gagaaaacat tgaaaaagga cataaatcca tggcgtatag cctgacattc
    11461 gtcaacccag aagcaacatt gacagatgaa gaaatcaata aagcaatgga aaaagtcgaa
    11521 aaagcgttgg tagaaaacct agaggcaagt attcgctaag aaggaaaata tagtaagtaa
    11581 cgatcaagta gcttttgaag accatacatt tgaaaaaaag atgtgacaca acttcgtcac
    11641 atctcttttt ttgattttaa aaaaggaata gcacagagac gagataaaaa aacaggcctt
    11701 tacagcctgc ttttttagag taaatgaatt aatggaagta attgattccc attgctgatt
    11761 ttacttcaga aagtgtttgt gcagctactt tagcagcttc ttcactacct ttacgtaaga
    11821 tttccataac agctgaagga tctttttcca attcttctcg gcgcgcacgg atcggtgcaa
    11881 attcagcttc caatacatca atgaggtatc gtttgatctt tacatcacca agtccgccgc
    11941 ggcggtaatg agctttcatt tcttcaattg cttctttatc tgttccgaat acatccaagt
    12001 aggtgaagac catattccct tctacttgtc caggatcttg tacatggatg tggtttggat
    12061 cagtatacat actcatgact tttttctgta agacatcggc aggatcggag atataaatac
    12121 cgtttcctaa cgatttactc attttgccat ttccatcgat tccaggtaat ctacccattc
    12181 cttttggtgg gaatacgcct tttggttcca ccaagacttc accatatgta tgattgaagc
    12241 tttgtacgat ttcctgtgtc tgctctagca ttggtttttg atcctcacca acaggtacaa
    12301 gattcgcttt gaatgcagtg atatctgctg cttgagaaac aggatagata aagaatcctg
    12361 taggcacgct ttcaccgaat tttttttgtt cgatttctgt tttgacagtc ggattgcgcc
    12421 gaacgcgtcc tacgctgact aagttcaaat aatacatggt taattcagct aattccggaa
    12481 tttgtgattg gataaataaa gtagattttg caggatctaa tcccacggct aaatagtcta
    12541 aagcaacttg caagacattt gatgaaacct tctcaggatt tttagcatta tctgtcagtg
    12601 cttgcatgtc agcaatcatg acaaacagtt ggttgtcttc atctgcctgc atagccactc
    12661 gggtttttag agaaccgacg taatgcccta aatgcagctt cccagttggt cgatctcccg
    12721 ttaaaataat atttttcaaa aaagacacct ctttacgatt tgtaagtctg agcacttaat
    12781 atcttaccgt ttttcctgaa aagaaacaac agttcctaag gaaaaaacaa ggaaagagga
    12841 taaaataact aatagattag aaatggaaga aatatttatg ggagaataaa cgttctaaga
    12901 aactttacaa attactaaac tatcagatat aatgtatttt attatacatc tttaaaagaa
    12961 atcttactaa ataataatgg gagtgtaatt ctatggcaat gattaaattt gatcacgtgg
    13021 ataaatatta tggcaagttc cacgcattaa aaaatatcaa tctcgaattc gagaaaggag
    13081 aagttgtcgt tgtcattggt ccttctggtt caggaaagag cacgatgctt cgctgtatca
    13141 atggattaga gacgatctct tcaggtaagc tgctgattaa tgatactgac ttgcatgata
    13201 aaaagacaaa actgacagaa gtccgaaaaa acatcggcat ggttttccaa catttcaatt
    13261 tgtacccaaa taaaactgtt ctggaaaaca tcaaattagc accaatcaaa gtgttgaagc
    13321 aagaccaggc aactgcagtc aaaaacgcgg agaaattttt gaaaactgtc aatatgttag
    13381 ataagaaaga ttcttatcct tccatgttat ccgggggaca acagcaacgg gtggcaatcg
    13441 ccagaggttt agcgatgaat cctgagatgc tgctgtttga tgaacctact tctgctttgg
    13501 atccggaaat gattggtgat gtattagacg ttatgaaaaa attggctcat gacggtatgt
    13561 caatgattgt cgtgacacat gaaatgggct ttgccaaaga agtggcggat cgtgttatct
    13621 tcatggctga tggagaagta ttggaagaca gcagaaatgt gagagacttt ttcgaaaatc
    13681 caaatgaagt ccgagcacag caatttatca gtaaagttat caatcattga taggaggaaa
    13741 aagatgcgca ggttaaaaaa aatacgcctg attttccttt taggcattgc ttttcttgca
    13801 ctagccggat gtaaaggaaa tgggttggca aatgaagata ttttagagcg aagcaaatct
    13861 accaatgaaa tcatctgggg tgtcaagtat gatactcgat tatttggaat gatggatatt
    13921 gagagcagaa ctgtacaagg atttgatgta gatattgcaa aagcaatcac taaaaagatc
    13981 cttggtgaca atggaaaaac agaatttgtt gaagtcactt ctaaaactcg gattccatta
    14041 ttgaaaaacg gaaatatcga tgcaattatt gcaacgatga cgatcacaga tgaacgaaaa
    14101 aaacaggttg acttttcaga tgtctatttc gatgctggcc aagctttgtt ggtaaagaaa
    14161 ggaagccaaa tcaaaagtgt agatgatttg aatgcttcta caacggttct agcagtcaaa
    14221 ggttctacat cggctgcgaa tatccgacag catgcaccag atgcaaaaat tttagaatta
    14281 gaaaactatg cagaagcatt tactgcttta caatctggtc agggagacgc gatgactacg
    14341 gataatgcga ttttgttagg gattgcagat gaaaatccag agtatgagtt agttggtgga
    14401 acgtttacta atgagcctta cgggattgcg atcaacaaag ggcaagaaaa cttcttgaaa
    14461 gctgttaacc aagcgctaga ggaaatgcat gcagatggca catacgataa aatctatcaa
    14521 aaatggtttc caaatgaaac tgaaggaaaa gtagagtaag ggggagaggc aatgagcgaa
    14581 ttgatacaaa cttatggacc aatctttgtc gacgggttca agatcacatt gttttcgagt
    14641 gtcttagcac ttgtgtttag tttgattatc ggaacgttaa tggcaatctt ccaattatca
    14701 aagaataaag tagtgagcgc cttagcaaag gcatatgtag aattttttag aaacattcca
    14761 ttattaatca tcgtgatgtt tttttatgtt gtcgtaccga tttattggtt taatctagat
    14821 ggattccaag caggtacgat tggattaacg atctatacat ctgcttttat tgctgaaaca
    14881 gtacgttcag ggattcaaac cgtaccaaaa ggtcaaacag aagcaggact atcttctggt
    14941 tttacctatt cagaaatcat gcgttatatt gttttaccgc aagcattcaa aatcgttatc
    15001 ccgccattag gcaaccaatt tatcaacctc gtaaaaaact catctatttt agcaatcgta
    15061 gctggtttag atttgatgta tcaaggagac ttgatcgcta gtgaaacatt caataccttc
    15121 gatacgtata tcatcgttgg gttattttac ttagttatca cgctaccgtt atcttactta
    15181 atggtttatc ttgagaaaaa atgggcggtt cgttcataga aggaggggaa atacatggat
    15241 tttagcggcg ctttttcatg gatgaatatc cgttttttat tggaaggatt aaaggttact
    15301 gttgaagttt cattgttttc gatcattttt agtttcttga tcggtggtct gacaggtgtg
    15361 attcgttttg cgaatatccc tttcttttcc aaaatccttg gtgtagtgat tgatattata
    15421 cggaatcttc cattattatt gatcattttc ttcacttact ttgccttacc tcaaatcggc
    15481 attcagatga acatcttctg gtccgctgtc gcagcattaa cgatttttga gtcagctatg
    15541 ttgtcggaaa ttttccgagc tggactcaat gctgttccaa aaggacagat ggaagcagga
    15601 ttatcaacgg gactgactta tgtagaatcg atgcgaacga tcgtgttacc gcaagcattt
    15661 aaatcaatga tcccggcaat tgtgagccaa ttgatttcac tgatcaaaga tacttcatta
    15721 gcagtaatca tttctttacc tgaactttct catcaggcac gtattattta tggacagaac
    15781 accaattatg tgcttccgat gttcatcatc atgacactta tgtacttcgt ggtatgttac
    15841 gcgctttctc tgctttccgg ttacttggaa aagagacggt acagttatta atagatgatt
    15901 cttttagcgg ttgtggcaaa ctaaagtcac aaccgctttt cctctttcca ttcgaaaaaa
    15961 agagatttcg attgttttaa gcaagtaaga gcacttgaaa attcacgcta aaaagtacac
    16021 aatagggagg atagaattta ttgaaaaaaa ggatgatacg attgacagat aatcgcccta
    16081 tcggatttat tgattcaggt gtcggcggct tgactgtagt aaaagaagcc ctgaaacaat
    16141 taccgaatga aaatatttta tttgtaggag acacagcacg ctgcccatat ggccctagac
    16201 ccgcggaaca ggtaatccag tatacttggg aaatgacgga ttatctggtg gagcaaggaa
    16261 tcaagatgct ggtgatcgcc tgcaataccg caactgcggt ggctttagaa gaaatcaaag
    16321 ctgctctttc tattccagtc atcggtgtga tccttcccgg tactagagcg gcagtaaaaa
    16381 aaacacaaaa taaacaagtt ggcattatcg gtacgattgg tacggtaaaa agtcaagctt
    16441 atgaaaaagc actgaaagag aaagtaccag aattgactgt gacaagtctt gcttgtccca
    16501 aatttgtttc agttgtcgaa agtaatgaat accattcatc ggtggcgaaa aaaattgtgg
    16561 cagaaacatt agctccttta accactaaaa aaatcgatac attgattttg ggatgcaccc
    16621 attatccatt attacgcccc atcattcaaa atgtaatggg agaaaatgtt caactgatcg
    16681 attctggagc agaaacagta ggtgaagtat ctatgctgtt agattatttc aatttgagca
    16741 attcaccgca aaatggtcgg acattatgcc agttttatac aactggctct gccaaacttt
    16801 tcgaggaaat agctgaagac tggcttggaa tcggacactt aaatgtagaa catatcgaat
    16861 tgggaggaaa ataaggatgc gtcatgacgg gagaaaagcc caagagcttc gcaaactgga
    16921 gcttcaaaca aatgttttca aatatccaga aggatcggta gtgatccgtt ttggtgatac
    16981 aacagttatt tgttctgcaa caatagaaaa ttctgttccc ccttttttga gagaaacagg
    17041 aacaggatgg gtgacagcag aatatagtat gctgcctcga gcaacgagta cgagaaatcg
    17101 tagagaaagc agcaaaggga aactttctgg tagaacaatg gaaatccagc gtcttattgg
    17161 tcgttccttg cgtgcagtag tagacttgga aaaattagga gaacgtagta tcatcgtaga
    17221 ctgcgatgtg atccaagcgg atggcggtac tagaacagca agtatcactg gggcatttgt
    17281 ggcattgaaa ttagccattg aaaaactttt aagagaaaaa gaattgtcag aagatccaat
    17341 aaaagaacat cttgctgcag ttagcgtagg aatcttacca gatgggactt gcgtcacaga
    17401 tttggattac caagaagatt ccgcagcact tgtagacatg aatcttgtaa tgacagaatc
    17461 tggaaaattc gtggagattc aaggaactgg agaagaagca acgtttgatg gtgaacagtt
    17521 gaatgaaatg ctcttttttg gaaaaaacgc gattgaagat ttgatcaaag agcaaaaaca
    17581 tgctttgcta acagagtttg ctcaaaatga tgagcggata gaagaaacaa aaacaatcat
    17641 cattgcgaca agaaatccag gaaaagcaga agagtttcga aatatgttca aagaagcggg
    17701 atatcatgta aaaacattac ttgactatcc tgaattgcca gatgtggaag aaactggcag
    17761 cacatttgaa gaaaatgccc gattaaaagc agaaacgatt gcacaattgc ttgatcagcc
    17821 agtcttagca gatgattcag gtttgaaagt ggatgcttta ggcggtatgc caggaatcta
    17881 ttctgcacgt tttgcagggg aacaaaaaag tgatgcgggg aataacgcca aattattata
    17941 tgaattgaca gatgtgccgg atgaaaaaag aacagcacaa ttccattgta cgttagtatt
    18001 cgcggcaccc aaaaaagaca gccttgtagt agaagcggaa tggcccggtc gagtagcccg
    18061 tataccatct ggcgaaaatg gctttgggta tgatccttta tttatcccag aaggtaagaa
    18121 acaaacagca gcagaattat cgagcgaaga aaaaaacaag atcagtcatc gggctcaagc
    18181 aatgaaaaaa ttgagtgccg agtggaaaca atggctggaa ggagagagat agatgcggta
    18241 tttagtagta agtgataatc atggagacag agatatcctc gttgacttag tagatcgtta
    18301 tcgttctgaa gtggatatct ttttccattg tggtgattcg gaattagaag cttctgaccc
    18361 attatgggat acattccaag tggttcaagg taattgtgat tatggaccag gattcgaaca
    18421 gaaaaaagtg atccaaactg gtcaggatac agtattcatg actcacggtc atttatcgaa
    18481 tgtccgattt ggtttgacac agttagcaat cgaagctcga gctgcaactg ccgatatggt
    18541 gttttttggt catacccatc aaataggctg tgagatggat cagaaaatcc tgtttttgaa
    18601 tcctggtagc atttctcagc ctagaggccc gatccaaatc ccatcatatg ctcttatcga
    18661 aagtttagat gaccaaattt ctgttcaata ctataaccgt gcccatcagg cagttgaaga
    18721 gttggccttt tctttttcga gagagcgctg aataaaaaaa ctctaatttt taataaaaat
    18781 aagctttatt ttatgaattt tgcatccggt ttcggtaaaa tagtagtgtt gaatgaaata
    18841 gaaatggagg ccatcagatg attggaccta tagtaagaga aatgctgctg gaaaatcagg
    18901 aaacgttttt gattcctgca gaaaatgtgg caaatgttat gtatcatcat ccattgtccc
    18961 atggtttact tgtgttatcg aaggtcggtt attccaaaat tcctgtatta ggaaaagatg
    19021 atcactttgt aggactagta agtttatcag atgtggtcaa taaaatgatt gacttgcaaa
    19081 caatcagcat ggacccgtta gaaggactaa cggttgctga tgtgacagaa acagatgttc
    19141 cgacaattga tgaaaattgg gaactagaag aagtactcca tttgttagtc gattcgcctt
    19201 ttttaccggt tgtcacagaa gatcggattt tcaaaggaat cattacaaga aaagagctgc
    19261 ttaaagccgt aaacttcatg gtgcatgaat tagaacgtcg taatatattg tctccgaaaa
    19321 tcgaagaatt aaaagaacaa atcgatatcg tgagctaaag aacgagaaac taaaagataa
    19381 tcaaaaaata agtagaaaaa acagcgtacc agttttgact ttatcttgaa aaagaggctg
    19441 ggacaaaagc
//
LOCUS       DACECR010000053        19352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.053, whole genome shotgun sequence.
ACCESSION   DACECR010000053 DACECR010000000
VERSION     DACECR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.053"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            170..364
                     /locus_tag="IWO17_001707"
     CDS             170..364
                     /locus_tag="IWO17_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290584.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            633..932
                     /locus_tag="IWO17_001708"
     CDS             633..932
                     /locus_tag="IWO17_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290585.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1029..1463)
                     /locus_tag="IWO17_001709"
     CDS             complement(1029..1463)
                     /locus_tag="IWO17_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0290586.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1695..2405)
                     /locus_tag="IWO17_001710"
     CDS             complement(1695..2405)
                     /locus_tag="IWO17_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0290587.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2395..3255)
                     /locus_tag="IWO17_001711"
     CDS             complement(2395..3255)
                     /locus_tag="IWO17_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ0290588.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3260..3898)
                     /locus_tag="IWO17_001712"
     CDS             complement(3260..3898)
                     /locus_tag="IWO17_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ0290589.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3913..4212)
                     /locus_tag="IWO17_001713"
     CDS             complement(3913..4212)
                     /locus_tag="IWO17_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0290590.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4255..5739)
                     /locus_tag="IWO17_001714"
     CDS             complement(4255..5739)
                     /locus_tag="IWO17_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0290591.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5759..6220)
                     /locus_tag="IWO17_001715"
     CDS             complement(5759..6220)
                     /locus_tag="IWO17_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0290592.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6238..7305)
                     /gene="ulaG"
                     /locus_tag="IWO17_001716"
     CDS             complement(6238..7305)
                     /gene="ulaG"
                     /locus_tag="IWO17_001716"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ0290593.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7523..8293
                     /locus_tag="IWO17_001717"
     CDS             7523..8293
                     /locus_tag="IWO17_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0290594.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8566..9393)
                     /locus_tag="IWO17_001718"
     CDS             complement(8566..9393)
                     /locus_tag="IWO17_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0290595.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9961..11196
                     /locus_tag="IWO17_001719"
     CDS             9961..11196
                     /locus_tag="IWO17_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0290596.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12195..12455)
                     /locus_tag="IWO17_001720"
     CDS             complement(12195..12455)
                     /locus_tag="IWO17_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0290597.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12482..13783)
                     /locus_tag="IWO17_001721"
     CDS             complement(12482..13783)
                     /locus_tag="IWO17_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0290598.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14075..14533)
                     /locus_tag="IWO17_001722"
     CDS             complement(14075..14533)
                     /locus_tag="IWO17_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0290599.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14546..15871)
                     /locus_tag="IWO17_001723"
     CDS             complement(14546..15871)
                     /locus_tag="IWO17_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ0290600.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15888..17300)
                     /locus_tag="IWO17_001724"
     CDS             complement(15888..17300)
                     /locus_tag="IWO17_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0290601.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17317..18081)
                     /locus_tag="IWO17_001725"
     CDS             complement(17317..18081)
                     /locus_tag="IWO17_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ0290602.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18185..19060)
                     /locus_tag="IWO17_001726"
     CDS             complement(18185..19060)
                     /locus_tag="IWO17_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0290603.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         6159 a         3973 c         2975 g         6245 t
ORIGIN      
        1 tttaagaaca atagcctttt attatatata ttattcaaat tagttatatc gagtgaaaaa
       61 aactttcaaa ttgattcttt ttatttatca taaagatata gatacataat cattttcact
      121 tacatatatt tgaatattaa ctaaaaacag caagaaagaa gaggatcaaa tgtattatgt
      181 agaagtaaaa acaaaaggcg tcaaaaacaa acaatatgtg aaaggtatca gtaacgaata
      241 tcctttactt ggttcatgga aagaagctgc tcctttttcc aaaccatgtg ccataaaaat
      301 aaagaacgag ctagaaaaag aactgacttg tggtaaagct gttgtagata tcatcgaaaa
      361 gtaaatccaa tgtatagaaa ggaagatcac acatgaaaag cgacgtacaa attgctaaga
      421 ttatagaatt agaagaagaa gacgcagtgt tgacaattga aaaaaaataa atgtttgtat
      481 accatatgat tatttcaata tgtatcctgg aattggagat ctcgtagagc tatatcaaga
      541 tgaatcaaat atacttgtca ttcatcatga aatatagaga cttataaaat tgtttatttt
      601 tataatgtat cagtatagaa aggaaataaa acatggatta tactgttgaa tttgacagtg
      661 aactacttaa gcaatttcca caagaagaac atttcatgta taaagaagca atttcccaca
      721 aagtgacaga gtatttggag cataattttt ttcgtataaa gccagtgcgt actcgttcgg
      781 caaaaggaat atacgagatg aaagtcaaaa ttaataaaaa aaattatcgc atagcatttg
      841 ctttaaaaaa aaatcatgtg ttcatttttt atattagttc gaatttgcaa aaaattgtat
      901 ttgataaaga ggtgagtaaa aaaataaaat agctgagcat tggaggatag attgagtgat
      961 actaagtcta tcctcttgtt tttctaaaga actaagctag aattttttct tttgtacgtt
     1021 attatatgtt atttgtcatt accaaaaagc accttttttc ttctagtatc tatgacataa
     1081 gaaataccac ccatttcctt tacttgaaca aatcctactg attcataaaa tttttttgtt
     1141 ttctcagtat catctgtcaa taattcgatt tggactggtc cataactagt gatcttctcc
     1201 aaagcttcat tcaaaagcac tttgcctatt ccttggcgct gatacctcgg atgaatgagg
     1261 atatcttgaa taaatgcgat cgtcgcaaaa tcagtgatcc aacgaacgat tccgataatc
     1321 ttcccttccc cattacgctt tatcagagat tcagattgtt caaatgctct agctaacctt
     1381 gcagtgtctt ttgtgtaatt cgtccaacca acgctcttat ataattcaga aatctcatcg
     1441 atcataaggg aatcaatatt catttttttc ttcctttctt atacaattct ccctctaata
     1501 atacagttct ttgactattt aatctatcac atataactct cctgtaaaga ttctctcgat
     1561 tccttaaaat tcctctttcc ttttagaatg catacttttt gataaataaa aaggttgtga
     1621 taaattttct tcacaacctc tgtttctctc tatcaagtgt tatcttttaa ttttctaccc
     1681 ttaccctagc atctctattg cccgtagtat gcatactttc catgtttgcg aaaataatgt
     1741 ttatctagta aatattgcgg gatttcacat atattggggt tgatcagctc gttttctttg
     1801 gccatcagac aaatttcatc taagatcaaa ctgttttcta ctgctttcat aggtgaatcc
     1861 ccccatgtga acggtccatg tccatagaca agtactccag ggacttcgtt tggatctaat
     1921 tttctttcct taaacgtctc gacaatcaca ttccctgtat gaacttcgta agcttctctt
     1981 acttcttctt tcgttaattg gcgtgtacaa ggaaccttac cataaaaagc atcagcatga
     2041 gtcgtgccat aagctggcaa atcgcgtcct gcttgcgccc acatgacgga gtgcgtggaa
     2101 tgagtatgag taatagcagt tatatcttca aaagtttgat acaaaacaac atgtgtgggg
     2161 agatctgaag atggttttaa gctatcttct tctattatat ttcccgacaa atctgttacc
     2221 accatttgat ctgcttgcat gcattcatat ctgacaccgc ttggcttaat gacgatgact
     2281 cctagctcac ggtccacttc actcacattg ccccaagtca atttgactaa tccgtatcgc
     2341 ggcaatgcca gatttgcttc aaggacacgt tgcttcattg ctgcaatttt ttcttcacaa
     2401 gacatcatag ccagcctcct tcaatttaga acttaagtag tcttttgcct gctggatttc
     2461 tcttcgaaag tcttggcttt tttcactcca catttcaatc agaaatgttc catcataatc
     2521 tagacgtttc aaatgtctta ataatccaag aaaatcaaca caaccgctac caaaaggaac
     2581 atcacgaaac tggcctgaat attcttttgt tacagggtaa gtatctttta agtggatagc
     2641 agtgatataa tcgatccctt tctcaagttc tcgagccgga ttattttccg gccatgcgga
     2701 caaattgcct aggtctggat atacttgtag aaaaggtgaa tgaatctgtt ctttgatttc
     2761 taaaaatttt gaaatggagt tcataaatgg atcatccatg atttccacgg aaagaatgat
     2821 gccatattta gaagcttcct ttacgctttc ttttattcct tcgataaagt cttctctgga
     2881 attcattgat ttctcttcat aataaacatc gtatccagct atttggatta ctttgacgct
     2941 tagattatgc gccaaatgga ttgctttttt cattagttgt ttcgctgctt ttttcttttc
     3001 tggatcagca gaaccaaacg gaaatcgccg atgcgcactt aaacaaatcg aatggatacg
     3061 tacaccggag ctgaacatct cttctctcag ctcagctatc ttttcttcag accaatctaa
     3121 tcgtgctaac cgctcatccg tctcatcaat cgacatctcg ataaaatcga agtccatttc
     3181 ttttacaagg gaaaaacgtt ctttccatga aatatctttg ggtaacgctt tttcataaat
     3241 cccaatttga gccatttcct tacccccaga tacgaatgat ctcatctttg aagttttgtg
     3301 cggccttcat cggatcattt gctgctgtaa taccacgacc agtgataaag gtatacacat
     3361 cgatcccttc aaataagtgt aatgtgtgtg gatttaatcc accagttaca gaaacattaa
     3421 agcctagttc aatcagtttt ttgatcttcg ataaatcttt ttcaccccaa gtttcaccag
     3481 ataacagcgc atcacggctt tgatggtaga ttacctgccg gataccgatg ttatgccaat
     3541 ccatggcttg ttcaaaagtc caattgccgt agagttctac ttgcaattca cctatttctt
     3601 tttgtgctgc ctccatagtt ggaatcgttg cacaacaaat gactgtcatc cagtctgctc
     3661 ctgcttttgc aacattactc gccactgttc ctccagcgtc tgcacatttc gtatcagcga
     3721 ctagtttttt atctgggaac atactgcgaa tacagcgaat cgctttttgt ccttcctgta
     3781 agcaaagaat tgtgcccact tcaataatat ctactacatc accaactttc ataacatctc
     3841 ctaatgcatg ttctaagtca ttgtgatcta acgcaatttg caaattcggc ctgctcatct
     3901 tagttccttc ctttacttgt ttttttggta ccactttgtt tcagcaaatt tttcactgat
     3961 ttctttgact gacatcacat ttttgatacc gataactgta actccttttt tctctgcctg
     4021 agtaaacata ttcaaaaaat tgactggcgt aaaaactaca tcgtattggc cagctgaact
     4081 ttttccctct gaaatggcac aatgatggat tttggtaatt ggtacgttta gttctttcat
     4141 tgctttttcc acacttctca tcatcatcaa gcttgttcct gatccatttg cacacgatac
     4201 taaaattctc atcttaatcc ctccattatg agtgttgtct aatcagattt tcttttatac
     4261 tctctgcttt tctgcatatg tgtcatagtc ttctacaatc aaaaaataac catcaggatt
     4321 tttccgatat tgtaactgcg gaatagccaa taaaccaatg acaactacgg caattccagc
     4381 ataacctagg actttcatga ttactgtaaa caatggccaa gctgttgccc agtcgaacat
     4441 cccgatataa ccaccgtatg ttgacatccc aatccatgta gcgaataatg ctgaaccgcc
     4501 tacttgtatg attcctgaaa gaaacgggaa caggcaagcg gctttgaatc cacctcggtt
     4561 attggcataa actgctataa cggcattatc aaagaacaac ggaatgaaac cggctatgac
     4621 gatcgttggt gatttcaata aaatcagtaa accaatcatc aaaaactgtc ccaaagcacc
     4681 aaacaagaac ccaatcgtta ccgcatttgg cgaaccaaag ccaaaagtag cagctacgtc
     4741 aattcctgga acagctcctg gtaaaatcgt gtttgagatt ccttggaaag attgagttag
     4801 ttcatcgacg aatgtccgaa cacctaattg caagatcgcc agatataccg caaaatttaa
     4861 agaagtcgtc ataatgtaga ataagaaact ctgcccttct tgcatgaatt gcgcttgaat
     4921 caaataatct ttgcctagta caatcagaat gatcccaaag aaaaatagca tgagtaacga
     4981 agtagcaacc atattctcat tgaaaatcga caagaatcct ggtaatttga tatcttcaat
     5041 ttttttgtct tgcttgattg atctattttt cttttcatcc cgctttttca tccactcagc
     5101 taattttgca aatataaaga ttccgaacat ttgctgatgt gccactgcaa atccggcacc
     5161 ttctgtcaat tcctgcgtga ttcctactgt taagttagaa gctactgtcc aataacaacc
     5221 taaaatcagt cccataaaaa tcaatgcttg tatcctacct agatcaggaa agcaaaacag
     5281 taaaatccaa aaagcggtag ctgcttgttg aacctgaacg tttccggtag tgaaaacgga
     5341 acgcagtttc gtatatttct gaaaacggac taataagata tttaaaacaa atgcaatcag
     5401 taataaaatc atcacatctc caaaggtacg gccaaatgtc tccattaatc cagcatccac
     5461 agcattttgt ccaaaataag gatctgtaac catggcatcc aaattgaacc gttcctttag
     5521 tccaaccaag attggccgaa aattattgac caaaccacca gagccaacag tcaaaataaa
     5581 gtaaccgacc gttgccttta agaatccagc gatgcattca tgaagcgggc gtttcagcaa
     5641 aatatagcct aataacacga taaaaccaat caggtatgct ggctgcgtca agatatttgt
     5701 tgcaaaatac gtccaaatgc ttaacaaaaa ttcccccatc ttttttctcc tctatcaatt
     5761 aattatattc tgccatcaca acttggtaat catcgatgga gttcgtagag atgagacgct
     5821 caaccaatcc atctgtcatt aataattcgg ataatttttg gatattttct agatgttctt
     5881 ctggatcttt tgctgcgagt gtaaaaaaca aactagccgt tttctcctca ttctcccctt
     5941 cactttcaaa acggattggc tgtttcaact tggtaaatga aatagctgtt ccaaaaacac
     6001 catcactttt ttctgaagaa tgcggcattg ctacttgagg tgcgatcaca atataaggac
     6061 cgtatttttc tacacaatgg atgatctcct ccacatacgt ttcatcaata atttccttat
     6121 cgatcaatac aagacagctt tcggcgatag cttcacgcca atccgtgatt tctttatctg
     6181 ggtaatgaat caattgatgg tcaaaaaagt atttcagcaa gaaactcccc ttctttctta
     6241 taaaaacgac ggaaacggtg tatcattttc aatcgagaag acatcttcaa atcctcgata
     6301 aaaattaaac tcaagatcat ctttgtttgt cggatagatg aatttcccgc ctacttgcca
     6361 gatataaggt ttaaatccat acttcaggcg atcctttttg aatttccaaa tttcagtgat
     6421 ctcttttgga tcagccataa aattagacca aatatcgtag tggacaggaa tcactacttt
     6481 tgcattcaat gactccgcca tgcgcagcat atcaacagaa gtaactttgt ctgttatccc
     6541 tcgtgggttt tcaccatagg cccctaaaca gacatcgatt ttgtgttcat taccatgttt
     6601 ggcaaataaa ttggagtaat gtgaatctcc tgcatgataa agattccctc ctgatgtttc
     6661 gaataaatag tttactgcaa tttgatccat ctcctgaggc atttttcctt ttaatacctc
     6721 ttctggatct tcacaagtga ccaacgctgt ccgatcaaac gcttctaaag ctactacatt
     6781 gatgtctttg attttgactg catcacctgg ctttactgta atcgtttttt cttcaggaat
     6841 tccccatttt tgccacgtgc gaaccacttc ttttggccca atgaatgttg cttcaggaca
     6901 attttgatga actgctgcgg ctgtatggat atctagatga tcggaatgga tatgtgtaac
     6961 gattaaagcg tctacatttt ttatcgcaaa aggatcgatt acgaaaggct gagtacgtaa
     7021 attaggctgc atattttgaa cgccactcat acgcatcatc tgatggcctt ttttcatcat
     7081 gccattccca tgactacgtt ttcccgtccc acaccataga tcacataaaa tattcgttcc
     7141 ttcatgactt ttcagccaaa ttcccgtaca gcccagccac cacatagcaa aagttccagg
     7201 atccactact tctgtttcga tttcttcatt caagtatgtg ccccactcag gaaatgtttc
     7261 tactaaccac ttttcttttg ttacttcatt gacagtttta ctcatttgct tgctcccccc
     7321 atatcgttgt tccaacttac aagtaaatat taatgtgtat cagcatccat gaaaaccctt
     7381 ttatatgacc gtttgttttt tatatgattt tctttcagta atcaatgatt gtttcagtat
     7441 aataaagaca gatatgaatg tttaaatgaa caactagtca aataaatcca tggatcaaga
     7501 tgtccctcag gaggcaaaag tcatgaaaaa ctcacttgtc aatattgaaa aacgtcaaaa
     7561 agaaattttg gaactgcttc agcagactga acaactatca actttcgaat tagctgaatc
     7621 ccttgatgta tctattagta cgatacgaag agatttaaat ttattagaag aaaaaaacga
     7681 gatcatacgc aattacggtt attgttctta taattacaaa aaccagactg attttgatca
     7741 atctggtcct gaaaggatta aacaagcgat tgccagagaa gctagtaaat acattaacga
     7801 ttatgatacg ctgtttatca attctagctc gactgcgttg aaaacactgg attttcttca
     7861 atctaaccat ttgacagtca ttacaaacaa cgtgaaaatc aattactctc cgcacaaaga
     7921 taattgtagt tatattttaa ctggtggaga gatgcgcttt ccaaaagaag cactagtggg
     7981 cgacatcgca ttaaatacga tttcttcagt caatgcagac atttgtatca ttggctgtag
     8041 cggagtcgat gtagtacatg gggtcacaac aaagatatta aatgaatcaa cgatcaacga
     8101 aatgatggtc aacaagacaa tcaaatgtaa gattttagtc gctgatcatc gaaaaatcgg
     8161 actgacatca aagttcaaga ttgctgatat ttcagtcttt gattatttga tcactgataa
     8221 attttgttct gaaaaagtat tagaggagat cagaaatacg gggataaaaa ccattcaagt
     8281 agattcattt taagaaccag gacgtaaata tgtaaatagg attatcaaaa aaatgaataa
     8341 caaatttatc gataaatgaa aaacacgcta cgctcaaatt gataaaggaa tcccaagatg
     8401 tatccatcca atcgatacca tcataatcta ttgcttcttt tataaccgtt atatgttgcc
     8461 taaataggaa tcaaacaata tcgaaaaaag gaggtatgac aaaagtcaca cctccttttt
     8521 tcgaataaac ggtattcaaa tcagaacact gatttcacaa ccactttatt tcaggtcgtt
     8581 ttcaatcacc tttaccgcgt aatcatttcc gttgaaacgc gctatttttt ttgcgtccat
     8641 atatctttct aaataatcat cttctcctaa ttgcttatgg caagaagcaa tttcataaag
     8701 caagtattcc aatgcataag tcgatttatg tttcttatta tactcaatac ccttctcaca
     8761 taattggatt gctttatcat aatcctcgat tttacggtaa taggtggcga tatcataata
     8821 tacaatgcag accatcagat tatcaaaatc agtctcactc aatattttaa gtgtacgctc
     8881 cacataaact ttcgcaaaat ctaattctcc ttttgacaag tagatcattg ccacgtttgc
     8941 agctgcttgt aagtgatgga tatctgtttc ggtcgtttgt tccaacactt gattaaaata
     9001 aaataatgct gcatctggtt ttttcgcata aagatgatca agcagtcctt gataaaataa
     9061 aatttgattt aaaagatcag aattacttac tttttcctga tcgatcgttt gtaaaatttt
     9121 ataagattct tggtattttg atacagaatt caatgattga atctcattta tttttttcct
     9181 gattttgtct tcttctgttt ctactaatat atcggagatc tcgacattca gctttttgca
     9241 aagcgcactt aagatattga tgcctggcga catattcttg ttttcgatat tgctgacagt
     9301 tgcttgttca caaatgcctt ctgctaattg tacttgtgtt aacccctttt tctttcggta
     9361 ttcttttagt aatgctccat ctactttaat caaaacaatt tccttccctt taaaaaatta
     9421 tttctccttt atatcataca caaaaaaaag ttctaaacaa ccaaaaatgt ttagaacaga
     9481 ataaaaattt taattttaaa ataatattca aattaaacaa ctggcgtatc tgctccttga
     9541 tttctttttg tgttatctga ataattatcg tttacagtta taacacatag tgaaaattgt
     9601 catggcgaga aagtctaaga atatttttaa acataactat ttctttctta cgtgtgtata
     9661 atgacgacag cacacctatc aattaatata attattttat ttttttatgt tctcatgttt
     9721 atttttaatg acacaacttc tcgtatttat ctggcacaac atataaatcg atcaaaaaat
     9781 tgttgcgtgt ttttttgaaa attggagttt caacttgatt acaaaattta aatttagaag
     9841 ttgtgaaaac agcgagagac agtttaatat tcaaaatata cataggctac tcgaacgctg
     9901 tgttttttag tatactagta agaaacgaag ggatagactc attccaataa ggaggatttc
     9961 atgaagacaa ttctttttgc tcctgcagta ttcaatttag ctgagacaac acgaatgatt
    10021 gaggttgcca aagctatgaa agaccgttat tactgtgtat tctttggctt ttctaaaact
    10081 tttgctcctt tgatcacaga aagtggcttt gagtatcatt tactagcgcc gatccttacg
    10141 aaaaaacaag aagaacaaat cataaaattt gaccagttgc gtacacttaa aaaccctttt
    10201 accaaagaaa tggttgcctc tcgtatcact agtgaacaga gtctgattgc taagtttgag
    10261 cctcgactga ttgtgacagg ttcgaatgtg acaatttttt tatcggcccg caataaaaag
    10321 attcctttag tatatgttaa accctatgct ttgagtgaac ctttttttca atcagagcat
    10381 gcgctgatgc cccacgagtt acgtttcttc ggccctttaa gcaatcccct ttggcactgg
    10441 acaaaaaagc gtatagtaaa tacccgatgg atccccaatg ccttcaaaca cgctgcacaa
    10501 aaagaaatgc ttcctttacc aaaaacaagt ttacaaatga tggatgctga tctaaatctc
    10561 atcacaacgc ctaaactatt tttggaagaa gaacagctgc cggaaaatta tcgaatcgtt
    10621 ggtccaattt ttgctaagct cagtacttcc ttgcctgaag aagtgctaac ttttatacag
    10681 cgaaagcaac aagaaagaaa acgtattatc tatttcgcaa tgggtagttc aggcaatccg
    10741 cgtatgttac gcaaaatttt gttcttctta caaaaacaac cggatctagc ggttattgca
    10801 cctattgact atctgcttga tgacattcaa tctgactcag aagatctttt aataacagaa
    10861 tatctgtcta gtcatctttt gcatgaattc attgattttt cttttatcca cggcggggaa
    10921 gggaccgtcc aaactgcctg tgaatccggc aaaccgttta tcggtgtggg gatgcaattt
    10981 gagcaaagct gtaatattga ctattgtgta aaatacggaa acgcaatcgc cctcacaaaa
    11041 ccagtgactg aaagaaaact gcagattgct ctgcgcgaag tttcttcacc caagattcgt
    11101 cagcaggcac gcacgctgca agccgttttt ccaataaatg gtccacaaca agcaattcaa
    11161 gaaatcgaaa ccttcctgag caaccaccca atatagtttc aagtagaaaa agctttttta
    11221 atagagactc atcagtaatt tcgctattcg agacccagac agtgaaaata caaaaaaagt
    11281 aaatagtgga ttgaaaagtt agagttactt aaattacggt gtctaaaaaa cacaaaatca
    11341 tccttaaaat aaaagatctg acgctatcca aatgagcata ggtagcgtct gccatttatt
    11401 cttagtcaaa ttctttgaca cctttctctg gttggatcat ctcaacgttt cttcaaaaga
    11461 aagaccataa taattaggca aatacatttt cttaaaatgt gtcatataga tctagaagca
    11521 tcgctttgta gagatttgtg ccgctagtcc cctcaacaat tactaaatca aatgtaggat
    11581 ttaccacttt atctttgata atcaacctcc ctcttctgtt gagacaatga tctcgcaatc
    11641 cgatgcatac tcctatataa actgccagca atccccacat attgagtaga tcatgtcaga
    11701 atgttctatg tcgtcagcaa cgatacacta aaagtcacgt tcaccatgac tgattgctat
    11761 tcccagcaca attatttcta tacacgtttc atgatttgaa taaacactca catcatacat
    11821 attaccattc atacaccata ctgcagcccc tactatatgg ttgtaagctc ccaaatctat
    11881 atttgacttg atcactttgg tagtagatct actaattcta aatccattgc tgataaaatt
    11941 tccatttttt cctccttctt tctgccaaaa cagttaaaac aataataatc tttatgtaaa
    12001 aaagggctgt gacaaaagcc ttgtcacagt cccatattcc gaataaacgg tggtcaaaag
    12061 cttgcatctg cggtaataag ttcaaccaaa cgaaaaataa gccaacattt cacaaaaatt
    12121 taagaagcaa ttttcgtaaa tttcttctta tttcttgaag ctaaccactt ctgttacaac
    12181 cacttttaga acatttaaag cttcgatgtg acaaaccatc tatgctgctc gatcataaaa
    12241 ttgatagcaa aaattccaca aagtacatat gtcccccatg aaaacaaaac gttcatatgc
    12301 ggtacaatga tctttggaag aatcacgatc aaaaatgatg ccagtgccca aatccatcga
    12361 ttgatttgaa tatgatatgt agctagcata ttcaatataa ggtgactagc cgttaataaa
    12421 aagataaaaa caaaaaataa aatcagccaa tacattgttt cccaccttct aaaaaccaaa
    12481 cttagacagt ctggacttcg ttttctccac gactttgttc atctgacagc atctttttat
    12541 cataagcttt tacaaatgga taatagataa taaatgctat caatgtgttg atcatgatca
    12601 agataacaac acgccagtca tttcctgctg aaagataacc gccgattgga gctggtaatg
    12661 tccatgttgg caaaacagta aatctattga ccatttctaa tttgactgca aaataactga
    12721 taatagtcaa aactaaagga actaagttga acgggatgaa catgtaagga ttcaacatca
    12781 taggagctcc aaaaattatg ggctcattga tattgaagat accaggaata atagaaaaac
    12841 gaccaacctc tttcaagaat tttgatttac agaagaatat aaacaaaaca gataccacga
    12901 tcgttgctcc tgcaccacca attgttaccg tccattgata aaactgttca ggagcgatat
    12961 agggtaattg ggtggcagct gtaccatctg ccaatgcctt tgcattctca tccaacatga
    13021 tcagccacat tggacgaaca acagccccta caatactcat gccatggata ccaaatgacc
    13081 acaacaaatg gatcaaaaac attggtagta aaacaccaag caaattattc cctaaaatat
    13141 tggttaaagg ctcgaataac gataataagg cagcgtttac atcaaagccc gcgacgcttc
    13201 gaatcagcca agtaattgtt aaaatgactg cacctggaat caatgcctca aaactacggg
    13261 caacagcagg tggcacttgt tctggcattt tgatagtaat atttcgattt ttaaagaaac
    13321 gatatacttc cactgcaaaa atcatagata agatccccgc aaacatccca gatgctccca
    13381 aagaactcat aggtaagacc cagccaagag gtgcctcttc aggagttaaa acgtttacag
    13441 gaacttgtaa cataaagaac gttcctaaag ataatatacc acctgtcaca ctatctaatc
    13501 catatgattt agctagatcc gaacccataa caaaagttgc ataaatagcc atcaatccca
    13561 tcgtcatcct aaagggtaaa acaatcgttg ccgaataagg ggcaataaat tcattccatc
    13621 caggaaacgg taaattgata aagaccataa aaaatgtccc aatcaaaatc aatgacaatg
    13681 ttgaaacaac cccattacga attgctaata aatgacgctg tgtaccaatt tttgccattg
    13741 gtgggacaag tttctcttcg acccatttca tcagcttttc cactaaaacc actccttttc
    13801 aataaaatat gaaccattca ttgcatgaca acgcaaagaa tgttcataac taattatata
    13861 attaagaaat tttatttcaa tatattctct taaataatca aatttatttt taagaatcct
    13921 gaaaataagt tttctaaatc ataggagcaa caaaaaaatt cgataattaa caaaaatcaa
    13981 aatgattaaa tctagtgatt gaaacatatc atttcaaaaa aattttgtaa attcacccat
    14041 aaatatcacc ctatttaact tttttcatcg tacactaaaa ttcaaaacaa ctatctttaa
    14101 tctttacttc ttggttttgt tttaataaat ttccttcaaa aatgcctgga gaaactacaa
    14161 tgatcaccgt atctttttgt gcttgctcca acttcttcaa atctataata gctaacagtg
    14221 tcccttcttt tacctcctgg ttactttcaa cccgtatctg aaacggactt ccttcgagtt
    14281 caactgtatc taaacccata tggatcagta tttttctgcc agattcactt tcgatcgtta
    14341 tggcatgtcg ggtaggaaat atattatcga ttattccttt aactggcgca tggatatatc
    14401 catcatgatt cgttatagcg tatccaactc ctatcatttt tgaagaaaag acctcgtctt
    14461 ttactgctgt taatggaata atttgaccac tagctacagc ttttagaatg tttcttgttt
    14521 taaagaaatg cactttttat cacctctact ttaatgctgg ccaatatttt ttgtttactt
    14581 tgatcatttc atctagtatt ttttttgcga ctctggcatt tggtacggtt tgattcaatg
    14641 taaatgcttg taaagctttt tgataagaac cttcaaagta agcatctact agtaacttct
    14701 ctactgcaac ttgtgcctcc atcaatcctt tatgaaaatc actgattggt tttcttagtg
    14761 taattggctc cacaccagtt gcaccaacat atgccggtat ttcaacaact gcatctgacc
    14821 gtagattcgg aatcgcccct tgattaggaa cgattagcat aaagcgacga cgatcatcat
    14881 tcaacaagga aatagctata tcgacgatat attgtccatg ctccccaaaa ttaaagttca
    14941 atacttcttc cacaataccg cggcgtattt tatcagccat ttctttggta tttttctctc
    15001 gtccatccat gaccatatta gcacgtgtat actctttgtc agtatgctca acgaccatat
    15061 caggatataa gtaatactct aagtaattat tcgggatatt acttggaaaa tttttcgtga
    15121 tgactgacat catttgaaag gttttttgcc aagttttatc tcctatatta aaatcggcga
    15181 cttgcatttc ttttgttatt agtttttcaa taatctctgg cataatttct cgttgcaatt
    15241 ctttatcata aatcgatgta taccagccaa agtggtttaa accataatac gtaacgatcc
    15301 aattcttacg atcataaccg taattcactg caatcgtttc ttcaattgaa atagtcatat
    15361 cacaggcatt aatcattttt acattcggat atttacgacg aacagcttca gaaacgatcg
    15421 actctggatt tgtataattc aaaatccatg ctttgggggc atagtcttgt atatgatcga
    15481 caatttccaa taaacctttg attgagcgca tcccataagc aaatcctccg agtccacacg
    15541 tttcttgtcc tactaatcca tgtttaagtg gaatcttttc gtccttttct cgcatctcca
    15601 atcctccaac acgaatttgt gagaaaacaa aatcgcatcc tgtaaaagca agctttggat
    15661 catccgttgc acgaatgcga atatttgaaa aaccttttct tttcagcata aatttaacaa
    15721 tcaagtacat atccatattt ctttcttcat caatatcata taaacgtatt tcagataatg
    15781 gcaaccgttt ttcattgttc aaaatggctt gaacgattcc tggtgtatac gtactcccgc
    15841 ctccagctat agtgataatc tgttttttca tcatcatctt ttcctttcta ttcttctaat
    15901 aatttattga attctgccat aacataagtc acttctaagc cgatgataat ttgataattt
    15961 ttttcagttg gtttaatgat cccgcttatc cctaattgct tgattcgctg ttcctcgacc
    16021 atggtgggat ccactacctc tacccgtagg cgtgtattac aactatagac atccaggata
    16081 ttggcttttc ctccaaacgc ttgtagcatt tttgttgcca tatattcata atttttactt
    16141 gctaacccta actctgtttc ttctaaggtt acttcattgc caaactctgc cttagcttca
    16201 cgtcctaatg tctgaatgtt tctcttattg atcaaataat aaaaagtaac gtaatacaag
    16261 gcaaaaaaga aaattccgat tggaagaatc aaaatagcat tggtagcgat tcgaaaattg
    16321 atgagataat caacaataca tgctccgaat gtaaaaccaa ttcgtatatt gaataagtaa
    16381 cagacaatcc ctgctaaacc ggcaaaaagt gcatggatca cgtacaacat aggagaaaga
    16441 aacatgaaac taaattcaac aggctcagtg atatttgcaa taaatgaagt tcctgcaccg
    16501 cttcccatga ttcctttgac catctcttta ttttctttat aagctgcttt atagatagct
    16561 agacatatag ctggtatacc aaacatcatt actacaaaaa aaactgataa aaacagtcca
    16621 gcctgaggat ctccgttgat aaagcgtgtc atctctcctc tgaaaacttc cttgctagga
    16681 gatgtatagc ttcctaaatc gtaataaata tacgtattca acacatggtg cagtccaaag
    16741 gggatcagca aacgattcaa aaacataaat atccccacac ctaatgctcc catatcaacg
    16801 agcagttttg caaaattatc aatcccaatt tggatgagcg gccagataaa gcccaaaaaa
    16861 acagctgcga ttgtttgtag aatcatcacc atagtcaatg ggaatttttc gcccgcaaaa
    16921 taagaaaaaa ctactggtaa tttttgttct ttaaagcgat tatacgtcca tgccgctact
    16981 aaaccggaaa taattccacc aaaaatcccc atattcactt ctgggtccat aatttctaag
    17041 ccttttttca aggtagcaat cgctaaaaaa ccagtaagtg caggtattcc tttgtcctta
    17101 tttttgacaa aaccgattgc aactcctata gcaaataaga tatccaagtt tccaaaaacg
    17161 gcatttccag ctgcaccaat tactgggatg ttcagcatat cgtcagctga taaacgcatg
    17221 atcaatcccg ctaatggcat cgctgcaact ggaagtagca tcgctcttcc aagcttctgc
    17281 acatttgatt taacattcat ttttattcct cctctattaa atatcctcat atgttactaa
    17341 ctcaatcaaa ggatcatttt tcactttttg agcaaatgct gaatgaatta aaaattcgac
    17401 ttggtgggta cgaggataca caaaacttga accatttaac aattcctgat ccaaatacgc
    17461 tgcgtgacac atgacttcga tcgttttatc ttctctcaat atcatgagta aacttgccaa
    17521 atattcttcc acactcgtat tgatttgtcg ttttttttca tcagtttctc ctacgacatc
    17581 gaaacaaggc tcaaaataat cgacatgacg aacttcatct tttcgttcaa agttgctccg
    17641 aaccggcaaa tcatattttt ttgctaatgt aacaactacc tcctgatttt catccaaagt
    17701 atgagtatga tgatgactgt ctaaatgagt tggtatgatc cccgcatgat aaattttttg
    17761 aatctgagca ttccattcct gatacagttg atctttgtca atgttaaatg gatgctgata
    17821 ataagataag gatctgaact ctccgctttc gataagagta tcgacagttt tgaggagtgg
    17881 tttgccacac gtcaatgtta aatggactcc tacacctagc ctaggcattt cctttcttag
    17941 tttcacagca tgctcgaaac ccggcatatt tgccatcaat gtagttgatg tcaaaattcc
    18001 tcgctgataa gcatccataa tcccataatt cacaccatgg ctatagccaa aatcatccga
    18061 attaaaaatc acttttccca tttcattctc cttctttttt ctactaaaat aggcgcaacc
    18121 aaataatcat agtccattta ctatgtgtca tttggttgcg cctatagata gttacgccct
    18181 tattttaata gattcttgct agatgaattg ccaaatagat gatttcattt tcttgaatgg
    18241 caagttgaaa ttgctgttcg atgatatctt taatctttag tgctgtacaa tacgctttgc
    18301 tatgtttaaa tgtttcagta aaaaaaccaa tattttctac tggcgtttct tcctgataaa
    18361 tcaagcgttt aaccaggaat ttgatatgaa ccattaagcg atgataagtc agtgattctt
    18421 tatcataaac gtttgaatag gatttttcta gatgggacaa aatcgtggtc gtcatttcta
    18481 ctatttttaa gctctttaaa ctattaggct ctcctagctc agcattaatg atatgcatag
    18541 tgatgaatcc tacttcattt gaccctaact cggtttgata tcgatgattg attcgctcga
    18601 ccacttctac tgctatatca tattcatttg gataaaggaa tctcatttct ggcaacataa
    18661 ttgctgtgac atctctatct tctctttttc gttgaatagc aaaataaata tgatctgtca
    18721 atgtaatata gatatttgaa tctaatggag aggagatcct agctgttatc agttcaatca
    18781 cttcttccgc aaaatccata aattcgattg gaatctgcga gactatctca ctgaatcgtt
    18841 tgatcttttc tttagaatct aatacaaata atttgtcagc atcctcaaca tcaaatggct
    18901 tactcacttt agcagaaaag ccaatccctt tccccatcgc aattttttct attccatcgt
    18961 catcgatcaa tacagcatta tgattcagct ttttcatcac atacaccgtt aattcacctc
    19021 ctctatatga taaaacaaaa caacttgcag actccttcat acatattttt gtctccaaac
    19081 aatttttaaa gacaaaaaac gacctaagaa agaacccttt cttaggtcgt gcctaattga
    19141 atagtagcac cctacataat ttacaaaaat agtttaacac ataataaaag cgttatcaat
    19201 atctttttta ctcgttttat gataatactt gtatcgaatt gatttggagg taattacata
    19261 aaaacaaaaa aaatatagca actctctata agaaagttgc tatattgata attataccgg
    19321 cggccggggt cgaaccggca cgtcctcaac gg
//
LOCUS       DACECR010000054        19279 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.054, whole genome shotgun sequence.
ACCESSION   DACECR010000054 DACECR010000000
VERSION     DACECR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.054"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            79..1125
                     /locus_tag="IWO17_001727"
     CDS             79..1125
                     /locus_tag="IWO17_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0290604.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IWO17_001728"
     CDS             1474..2106
                     /locus_tag="IWO17_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ0290605.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IWO17_001729"
     CDS             2694..4133
                     /locus_tag="IWO17_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0290606.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IWO17_001730"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IWO17_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="IWO17_001731"
     CDS             4895..5110
                     /locus_tag="IWO17_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290607.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="IWO17_001732"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="IWO17_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="IWO17_001733"
     CDS             complement(5770..6681)
                     /locus_tag="IWO17_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0290608.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="IWO17_001734"
     CDS             complement(6700..7704)
                     /locus_tag="IWO17_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0290609.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="IWO17_001735"
     CDS             complement(7701..9824)
                     /locus_tag="IWO17_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0290610.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="IWO17_001736"
     CDS             complement(9829..12276)
                     /locus_tag="IWO17_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0290611.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="IWO17_001737"
     CDS             complement(12263..12652)
                     /locus_tag="IWO17_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0290612.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="IWO17_001738"
     CDS             complement(12712..13215)
                     /locus_tag="IWO17_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0290613.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..13452)
                     /locus_tag="IWO17_001739"
     CDS             complement(13228..13452)
                     /locus_tag="IWO17_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290614.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13556..15467)
                     /gene="ltrA"
                     /locus_tag="IWO17_001740"
                     /pseudo
     CDS             complement(13556..15467)
                     /gene="ltrA"
                     /locus_tag="IWO17_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(16213..16332)
                     /locus_tag="IWO17_001741"
     CDS             complement(16213..16332)
                     /locus_tag="IWO17_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0290615.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            complement(16347..17531)
                     /locus_tag="IWO17_001742"
     CDS             complement(16347..17531)
                     /locus_tag="IWO17_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0290616.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(17790..18080)
                     /locus_tag="IWO17_001743"
     CDS             complement(17790..18080)
                     /locus_tag="IWO17_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290617.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18070..19212)
                     /gene="dcm"
                     /locus_tag="IWO17_001744"
                     /pseudo
     CDS             complement(18070..19212)
                     /gene="dcm"
                     /locus_tag="IWO17_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT         5643 a         3948 c         3430 g         6258 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctaacaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacatagc ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attgggcatt ggttaaaaac cattgatata tggataggta
    13441 agttcctgat attataaata tctcaaactt ttccccccat tcacacggag cgtgcgactt
    13501 tcaccgcact ccgcgttcca tcgtgtttat attgaaaagt atttatgtag tagacctata
    13561 tcaagtttaa ttcttgattg ccagctaata ctctatattt atttagtctt atgagctgga
    13621 ctttatttag cttcaataga gctatatatt tttgtattgt ttcttctgtt ttagcgtgta
    13681 ttagacggtg taccgcctct tttaccaaaa taagattttt ataatcgtca tttccaccag
    13741 ctccagttgg tacaatatga tgacaatgta tttcattagc ttgttgaaat tcttccagag
    13801 ttatagcaca ttttccatgt tgagcagaaa atagcgaaat tcgattgtct gcgtattcta
    13861 ttgtgcgatt ttccaaagat aatttcatta actggtacat cagtaattta tcaacactta
    13921 gattggtgtg gataaatttt cttccctcta gagtgtagca attcgctaaa tacacctttg
    13981 ccattggttt tctgtgttgg atatatccaa cagggaaaat aggttcgccc tcgccagcaa
    14041 cataccgaag cattttagat ttaccatatc ttgttgcctc ctttgtcgta agttgtctgc
    14101 ctgtttttct caatctgccc cttttgtttg tacctaatct gttagtgaag atagccatta
    14161 cagctctatt cagtttcata caatccagat ttacattcgt tgcaatctga taataatttt
    14221 gcatacctaa taccattgaa ttataaagtc ttgtttcttc taattccgtt ttcccttttg
    14281 agggagaagc aattttcttt gcctgtgata ccagcttgtc tttttggttt ttgaattgtt
    14341 tatcacttat atgtgattta acaactaact tctttcttct cggtatcatt ttaatcttga
    14401 aaccaagaaa gtcggagtat ctttttctaa catttacaat tcttgttttt ttcttgtgat
    14461 acttccaatt ttaatcgttg ttcaatccat tgagtgattg ctattttggc tttttcagca
    14521 ctttccttgt agcggcagaa aactctaaaa tcatctgcat aacgaaccat atacatttcc
    14581 tttaactttg ttttcttcat ttctttgtaa gcgttgcttt tacatgggct accgctttta
    14641 ttgatatgaa ccacatagtt ttttgtaaca ggattttcct gccattggct ttctacccaa
    14701 tggtctaact cattaagaac aatgtttgct agtagtgggg atattattcc cccttgcgga
    14761 gttcctttct ctggatatgt tattgtaccg ttagtcattt tgattggagc ttttaatatc
    14821 ctttttaaaa tgaatatcaa gtgcttgtcc cgtattccca tagcccatat ctgttttatc
    14881 agcttggaat ggttcacgtt gtcaaagaaa cccttaatat caaattcaat cacataatgt
    14941 agctggctga tttgtaggcg gttataggtt gcttttattg cattttcaac tgacctatta
    15001 ggtctaaaac catagctgtt ttcactaaat ttagcttcgc agattggttc taatacttgt
    15061 tttatacatt gttgtataag cctgtcccac atacaaggaa tacctagagg tctggtttta
    15121 ccatttggtt ttggtatttc ttttcgcctc actggcttag gtcgataccc atgtggactg
    15181 cctgcaacaa tgtatcttac ttttttgaca actgtttctg ctggcaattt gccaatatct
    15241 ttaattattg taccgtctgt tcctgctgtt ttactgcctg catttgcttt gatatttcga
    15301 tatgccagta atatattttc tctcgatagg ataatatcca taagatgagt gaaaatatct
    15361 ccattcttac ttttggcata taaatcatca aaggtgtttt gcatttcgta atattcagcg
    15421 tgtcttaaat cttctacaca caatgtttca tttttatttt taggcataag gcttcactcc
    15481 tttacttgaa attgagtgag cttttttatc ttactcgtaa tccttattga aacaacataa
    15541 tacttttcta atatatacac gacttgaggg cattcctcca ttctcattac aagaatttct
    15601 taggttcgcc ctctgctttt cagcattagt tcaactgctt attgttcttc gtcagtttat
    15661 tctcttgatg agaacccaat acctttcctc gttccgataa tcttatctat acatatatat
    15721 ccttaggtca ctagctataa gcctgctggc ttgataccgc ctgtaacgtt atatggcatt
    15781 tcataaccta tattcttact ttgccacacc agctacgatt tctcgtacct ttgcatttct
    15841 acaaagttca aatttagacc cgtacattcg ctgtttcgtc agttccttat agaacattct
    15901 aaccatagat attttataga cccccgacct gttggatacg ctacccacct ctggatagtt
    15961 ttcgatagaa gatatatgtt catcaactat tacggtatct ttcagccgtc tttaccgagc
    16021 ttcaaacatt ctgagtttcc tcatacaatg cttgtcggag gctcagggca ggcgtttcag
    16081 agcgttactt catcattcca cctgtcagat tatcagttct cctaataacc aaactttttc
    16141 gttcttttat tagtgcctta gcttttcact aaggaacgag tcgcacataa attttgtccg
    16201 aaattcatct tgtcactttc ctttcttttc cattgctcga tccgctgctg cacttgcatt
    16261 catcaagcac atcacagtaa caccaagcat actgccacca aataccaaaa aaatatcttt
    16321 tacaattccc atgacgattc ctcttttcat ataatcaaat tttcaattgc catactttgt
    16381 tgctccaaaa tctttttatg tcggtctgat aagcgtgcat tctgtaccat atcctggata
    16441 taggttgtcc gattcaatcg atccaactcc ttagccattt tccacgtagg ggcgacttgt
    16501 cggcctagcc aattcaaggt tcgttcaatg gtatacggtt ttggctcgga agttaattga
    16561 atctctttcc gattccgtcc aataaaacgc ccccattgtt cattcaaggg ccagttggtt
    16621 cgccttttac cttctacttt atccgcaaat cgcaagtaac gattgataat ggaaaacgtg
    16681 gtgctctcaa tgctacgacc ttttaacaaa tcttgaatcg catgaaaagc acggtcattt
    16741 ttcagtcgaa tctcaaaacg atttttcgtc tcagtttggt ctaatggaat ccctaacttg
    16801 atatattgtt catagtcttt ttcatacaaa cagaaataaa cctcactctt gagactgccg
    16861 atatataagg tattgcccat gccgtccttt tcatcagcct ttaaaagttc cccggaacga
    16921 taacttttaa aggtacgaaa caaggagata cattcttcct tctgacattt tttcgttagc
    16981 tcaggtatat ccaatagccc cactcgatca ttgattgcca agtccaagcg tttcatcacg
    17041 ccaccggcct ttagacaatc ctcgaaaaag tcgtaccagc tacgcttttg agcgagtaaa
    17101 aaagtttcaa attgtcggca acctcgcccc tttaattcta aaagcacacc tttatcttct
    17161 tcatgagaca acatcaccac aatatcaccc atgacatact gttcttgata ggagtaaaag
    17221 gcataatctt catggagcat atactcaaat ttcaaatgga gaatttcttg aataattttt
    17281 cgtgcatccg ttgtgggaaa gcgaatcctt acatagtcaa acagtaacgt caaaggcaaa
    17341 tcgggattaa agctttccag ttggtcaaaa attttcctca tggtttttat agatgcggtt
    17401 acgccaccat tttctatctg atttaagtac ggccgactaa gtccacaagc cgtagccaat
    17461 ttattttgtg aaacgccgta ttcgttgcgt cggtctttta atagccgata atctaaattc
    17521 cgttgtgcca agttctcacc tccaaattca aggtgttaac tttttcaaat gagttaacac
    17581 ctatacaaat tacttactat ctttgataca acaagcattc ttaatggttg cttacttatt
    17641 ttttgtgttt ttgtaccccc ctgttagata cgggggtgag acatcatggt cggctaacgc
    17701 cgtccatgaa caggcacagc accacggagg atttctgctt cgcgaatcct ccgcggtcag
    17761 cgcctaacag tcagttgtgt tggtttaggt tactcatctg taataccatt tacttccaac
    17821 tctgctttca tttctaacaa gtattgacga atatctggtt cttttgcttg gtccgcaatc
    17881 cgctcaaatt ctgcaattac ttgatttatt tcaattcgag catccgtctc ctcattcatt
    17941 aaatatggat cgccagcgac tttgacacca ttcttgcgaa tatcaaattt taaccgtcgc
    18001 aattgttctt ctaattctgg gtctgactga atctttccaa tcacccaata aatttctcca
    18061 atcaactcat cataaaccat gtacatccaa ctcttttaat cttctggcaa tatcaaaaat
    18121 gaccggaact gttacagagt ttccggcttg cttatacaac tgactatctg aattgacttg
    18181 agaatctcga tcaaaagccc aatcgggaaa gccctgaagt cgccaacact ctctaggtgt
    18241 tagcttccga atttgatact gatccgtgac gatcgcttgt tggtctcctg ttagtaaggt
    18301 atgaacactc tgttttccca gacgacctcg gcgacttgtg gaattaggaa aagccagatt
    18361 cacactgtct tctggcttca cctcttgata ttctgtttca ttttttcgaa tcatgatttt
    18421 tgtaccacct tgtttcgttg acaaggtggg ggcggttcca gtaacttcat aaactcgatt
    18481 accttgttca aactttcccg gtaatagtcc tgctacagag attccatccc ttgctggcag
    18541 tgcgattttg attccttcat cacctgttaa ggttggcgct acgccatctg aaccatagac
    18601 ttgtccaccc attcctcgat tggaaggatt gatatttacc aaatttctga tgttttgatc
    18661 agatgttgaa gacggtccaa tgataggaaa tacttgttcg gtacatgcgt ctcgagaatg
    18721 tccgataagg aatatccgct cccggctttg gggaatgtag gcctttgagt taagcaccta
    18781 ccactccacg tcatacccca atccatccag cgttcggagg atcgtctcga acgtagcccc
    18841 tccttcgtga ttgagcaatc ctcggacgtt ctcaaggaat aaaagcttag gtcggagaag
    18901 agtggtgaac cgagcgattt caaagaataa agtacctcga gtatcggaaa atctttgccg
    18961 ctttcccgca attgaaaaag cttggcacgg aaatccgccg caaaggatgt ccactggtcc
    19021 gagggattga ataaactcat ctgatacact tgtgatgtca tgcatttcca cctccttact
    19081 tgtatcatga attgctttat aactacgtct agcaaattta tcaatttcac aaaaaccgat
    19141 acaatgatgg ctcgcctgtt ccatacctaa acgaaagcca ccaataccgg cgaataaatc
    19201 taaaaatctc atacattacc tcattcattg caaagaaaaa agccaagaac attttgctct
    19261 tggctcatac attattaat
//
LOCUS       DACECR010000055        18840 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.055, whole genome shotgun sequence.
ACCESSION   DACECR010000055 DACECR010000000
VERSION     DACECR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.055"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(45..1364)
                     /locus_tag="IWO17_001745"
     CDS             complement(45..1364)
                     /locus_tag="IWO17_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0290618.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="IWO17_001746"
     CDS             complement(1361..2014)
                     /locus_tag="IWO17_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0290619.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="IWO17_001747"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="IWO17_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="IWO17_001748"
     CDS             complement(2724..3440)
                     /locus_tag="IWO17_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ0290620.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3564..3749)
                     /locus_tag="IWO17_001749"
     CDS             complement(3564..3749)
                     /locus_tag="IWO17_001749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290621.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3976..5249
                     /locus_tag="IWO17_001750"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="IWO17_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="IWO17_001751"
     CDS             complement(5356..6387)
                     /locus_tag="IWO17_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0290622.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="IWO17_001752"
     CDS             complement(6394..7224)
                     /locus_tag="IWO17_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0290623.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8027)
                     /locus_tag="IWO17_001753"
     CDS             complement(7221..8027)
                     /locus_tag="IWO17_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0290624.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="IWO17_001754"
     CDS             complement(8033..8857)
                     /locus_tag="IWO17_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ0290625.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="IWO17_001755"
     CDS             complement(8847..9947)
                     /locus_tag="IWO17_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0290626.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="IWO17_001756"
     CDS             10125..10910
                     /locus_tag="IWO17_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ0290627.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="IWO17_001757"
     CDS             10943..11326
                     /locus_tag="IWO17_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0290628.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="IWO17_001758"
     CDS             11402..11533
                     /locus_tag="IWO17_001758"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0290629.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="IWO17_001759"
     CDS             11502..11738
                     /locus_tag="IWO17_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0290630.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11816..12787)
                     /locus_tag="IWO17_001760"
     CDS             complement(11816..12787)
                     /locus_tag="IWO17_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ0290631.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            13439..15061
                     /locus_tag="IWO17_001761"
     CDS             13439..15061
                     /locus_tag="IWO17_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAQ0290632.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     PFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
     gene            complement(15347..16723)
                     /locus_tag="IWO17_001762"
     CDS             complement(15347..16723)
                     /locus_tag="IWO17_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0290633.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(16723..17379)
                     /locus_tag="IWO17_001763"
     CDS             complement(16723..17379)
                     /locus_tag="IWO17_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0290634.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(17389..18666)
                     /locus_tag="IWO17_001764"
     CDS             complement(17389..18666)
                     /locus_tag="IWO17_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0290635.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(18685..>18840)
                     /locus_tag="IWO17_001765"
     CDS             complement(18685..>18840)
                     /locus_tag="IWO17_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAQ0290636.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         5801 a         3888 c         3044 g         6107 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttatccag actgtagaca aagtccattt tatggatgat
    13381 gtctacagtc ttttctttta taatagattt atgaaaacga atgcaaggag tgatagctat
    13441 gatgtctaaa aacaatacga atggtagaaa tcaatttgcg atgcttacga ttgatgatct
    13501 agttcctcaa gatcatctcg ttcgaaagat tgatgccgcg cttgattttg aatttattta
    13561 tccaatcgtc gaagccactt actccgattt aggtcgccca agtatcgatc cagtgattct
    13621 gatcaaattg gtgtttattc agtatttatt cggtatccgt tccatgcgac aaaccattaa
    13681 ggaagtcgat accaatgtcg cttaccgctg gtttttaggc tactccttcg aggagaaaat
    13741 cccccctttc tcaacttttg gaaagaatta tgttcgccga tttagagaaa cgacggtgtt
    13801 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
    13861 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
    13921 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
    13981 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
    14041 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
    14101 agaaaaacaa tttgcttatt ctgtccatac ttcatgcgac gacaatggat ttattcttag
    14161 cacaatcatc actcctagaa atattcatga cagtcaagtc gcctttcagc tcgtcaaaca
    14221 atcgaagaga ctttttcctg aaatcaattg tgttgtcgct gatgctgggt ataagacacc
    14281 taagtttgtt cactttttga ctcatttgaa ccttcgacct tgtttgcctt attcaaggcc
    14341 caaagggaaa aaggagctat tatcaaaaaa tgagtttctc tatgacgagt attttgattg
    14401 ttatatttgt ccgcaagatc aaatgctcgc tttttcaacg gttactcgag aagggtaccg
    14461 agaatacaaa tccaatccaa aagaatgtgt gaactgtcct ctgctaaacc aatgtaccat
    14521 aagtaaaaat catcaacgag tcatcacgag acatgtttgg ggagatctga tggatgaagt
    14581 ggaacatctt cgcctaacag atctgaataa atcaatttac aaaaaacgga aacaaacgat
    14641 tgaacgaata tttgcggatg caaaagaaaa gcatgggatg cgttggacca agtatcgagg
    14701 cttggaaaaa gtcgcgacgc atacgatgct agtgtttgct gcaatgaatt taaagaaatt
    14761 agccacctgg ctatggaaag gcaaggagcc tttatttttt tgttcaaaaa ttcgaaacga
    14821 agtagataaa aagctgttcc aagctcgagt tacgagtttg gaacagcttt tgtctacagt
    14881 ctgcgatgtc ggagacatct cgttttctat taggaatcaa ctcaggttca aaagaagcct
    14941 cccggtctct gggaacatca atgtttactt taccaaagct cgttttaagc gtcttattgc
    15001 cataaccgtt tcgtcgattg tcatgtttct ttggctcttt agaatgggcg tcatacccta
    15061 aatggttatt caattctcct tgaagcattt tttcaaataa gggaccaaac acatctttta
    15121 aaacatcttg catatcctct acagattcgg gttgataagt attcaaaatg gattcagcta
    15181 acttctcagc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
    15241 ttttagaaaa ataaaaaccg tgaagcaacc acccgcctag gattgatggt tacttacacg
    15301 gtttatatta cactctcatt tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa
    15361 ttaataattc ttttggattc cttcttaata tattgaatga agaaataatc aatgatacaa
    15421 gaagtactaa actcataaat ataactacat aaatcatgaa tttaggtaat acattgatat
    15481 ctaaacttga taatgtcttg ttaaatcctt cagcttctgc cccaccacct aattggctag
    15541 atgcagattg tttagctatt tgtttagcta tatcaccagt aaccttattc aatatattat
    15601 tcccaagctt gtcagctgta tattgagcta agaaatagga accaacgaat gcggggattg
    15661 atataaatac catctcaatt aggaattgac caaaaatttc taattttgat atacctaatg
    15721 atagtaatac tgctatttct ttctttctag cattcatcca taagaataat aataatgaaa
    15781 ctacaactcc tgcaaatatt aatgagccaa aaaataattt atttgaaatt gagtaaatac
    15841 ctgatataga ttgttgtaat gcagggtaat ttgatgaact tttaatcaaa ttatattctc
    15901 tccaatttat atctaatttc ccaagatctt ttattacact atcaagattc ttatcacctt
    15961 ttacaaagaa tgttgcatct tggtatacag ctgtatcttc ggtattacca taaactttag
    16021 cagctgtatg aatatcagtg attaatgtat tttcgtatag ttcttgtgct gcacttacta
    16081 caccgctatt atgcccatcg aatagacctt taatttctac ttctactgtt tcatctgcac
    16141 ctttctcatt atcagcatca aataaattag attttatttt tatcttatct ccaactttaa
    16201 ggttattttt ttcagctaaa tctttatgca ttaagatttt atttttatct tcattttcta
    16261 aatgctttcc ttctactaat ttatatgctt ctgatacaaa ttttgtttct tttgttgagt
    16321 cgttaacccc agttaacata actgttcttt taaaattctt tgctctttca ggtgattgat
    16381 tcgcaagagt ctcttgagtt tcaataatat cataatcaac taaatctgca acactgttta
    16441 ttctttttac ataggagtct atactatctg tttgagatat ctttttaata tcttcacctt
    16501 ttacatttcc cccacctcta ggtgttcctg gatttacttg tctatttatc tccatagaaa
    16561 aactatttgt tatattagca aatgtttctt ttgaagctct gtccgtagca tctttaatag
    16621 ataaactaat gagacttaaa gttgacatag ataaaataac taatatgata attaatgatt
    16681 ttaaactttt tctagttaca taagcaaatg cattttttat cattattcaa cctccaaatt
    16741 cattttattt acttttttca actttttacc acttagttct aaaatgatat ccgcagaatc
    16801 tgctacttcc ttactatgtg ttacaactat tacacattta tttctatctt tagctaatgt
    16861 ctttaatata ttaattattt ctccagcagt aacactgtct agattaccag taggctcatc
    16921 agctagtatt attggagcat ctgataccaa tgctctagca atagctactc tttgttgctg
    16981 tccaccagat aatttcataa catttctttt tatttgtttt ttatctaaac ctagttcaaa
    17041 caagatactt tcatctgctg atttatttac taatctaata ttttcaattg gtgataaata
    17101 atctattaaa ttatagtttt gaaatactaa agatatatta ttttttctat gattactata
    17161 tcctttattt tgtatatctt cattcttaaa caatattttt cctgtttgag gtttatcaag
    17221 tccagcaagt aaggaaagaa gtgtagattt tcctgttcct gactttccta ctatcgcata
    17281 aaacttccca agttcaaatt tttgatttac tcctgacaaa actttttctt tagaatttgc
    17341 ataactatat gctacattct ttatttctaa tatatccatt attttctcct aacttatttt
    17401 tgataagatt tctttaggct tcttgattaa cattaatgaa gaagcaaata caactgataa
    17461 aacaataata cttattaata ttaaataact ttgtccaagt gttgttatat ttgacataaa
    17521 actactatta tttattaaac ttccaccgga aatcattgaa tcctctgagt taatgaatcc
    17581 atctacaatt acttttagta atacatttcc taaaaataag gaagatatta tacttggtat
    17641 cgatatgaat attaactcga atataaattg cattataatt tgtatcttag atgttccaat
    17701 agataagaat atacctattt cataaattct ttcccttaac catagaatca agattaatga
    17761 aagaacaacc atcccaccta acataataga ataagtcatt acttttatta tatattttat
    17821 tccactcact gactctaaag actcttcaaa cgcattagaa tctttttcaa caaaatactt
    17881 tgactcatca attttaagct ctttcaattt gtttaaggct aagtctgtag attctgcact
    17941 accagaatac attaaaattt tatttgcaat ttcattattt tctgaattat ttaatatctc
    18001 ttggcttgtt gaataatcta caaacaccat attttcacta aaatcagaag ataatcctgt
    18061 atatgtttcc tgttttttac cagaaaagat ccctataatt ttaaatttat gactttttat
    18121 ttttccactt ttttcaatat ctagtaattc aagatcaact tcatcaccta attttagatt
    18181 gttttgttta gcaaattctt catgaacaat aattgaattc ttatcatttt cttctatatt
    18241 tttaccttct ttaattgtaa atactccact actaaataaa atatttcttt tagtattatt
    18301 tgtagcttcg agtgaaacaa cattcttaaa ttcgtcagat aaatcttctc tatttattct
    18361 ttgttctccg ctaactactt tagcatcttt tagttttgct aatccatcat attgaattat
    18421 tttttcttca atttctttta atttttcaat atctttaaat tgattaacat taaaatattt
    18481 accatctttt tttgttattg atattgaaga attagaactt tcatataaag ccttttctat
    18541 ttcattgctt gatttcatta ttgttaaaca agaatacaag caagaaagaa ctatagttaa
    18601 tatgataaaa attattaaag ttctattttt ctttcttgta atatatgcta tagcattttt
    18661 tatcactttc ctaactactc ctctctattt tcttactcca taactaattg catccactgg
    18721 acaagcattt ttacatcgtc cacatctgat acactccaga tgattgcaat tctcaactga
    18781 atctttattc atttggcaaa cttttttaca ttttccacaa ttaatacact tatccttatc
//
LOCUS       DACECR010000056        18534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.056, whole genome shotgun sequence.
ACCESSION   DACECR010000056 DACECR010000000
VERSION     DACECR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.056"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            129..776
                     /locus_tag="IWO17_001766"
     CDS             129..776
                     /locus_tag="IWO17_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0290637.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1169..3814
                     /locus_tag="IWO17_001767"
     CDS             1169..3814
                     /locus_tag="IWO17_001767"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ0290638.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FYRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4124..5437
                     /locus_tag="IWO17_001768"
     CDS             4124..5437
                     /locus_tag="IWO17_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ0290639.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5427..6080
                     /locus_tag="IWO17_001769"
     CDS             5427..6080
                     /locus_tag="IWO17_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0290640.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6135..6827
                     /locus_tag="IWO17_001770"
     CDS             6135..6827
                     /locus_tag="IWO17_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ0290641.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6977..7177
                     /locus_tag="IWO17_001771"
     CDS             6977..7177
                     /locus_tag="IWO17_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0290642.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7431..9110)
                     /locus_tag="IWO17_001772"
     CDS             complement(7431..9110)
                     /locus_tag="IWO17_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0290643.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9112..9324)
                     /locus_tag="IWO17_001773"
     CDS             complement(9112..9324)
                     /locus_tag="IWO17_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ0290644.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9717..11447
                     /gene="efrA"
                     /locus_tag="IWO17_001774"
     CDS             9717..11447
                     /gene="efrA"
                     /locus_tag="IWO17_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ0290645.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11444..13204
                     /gene="efrB"
                     /locus_tag="IWO17_001775"
     CDS             11444..13204
                     /gene="efrB"
                     /locus_tag="IWO17_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ0290646.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13299..13496
                     /locus_tag="IWO17_001776"
     CDS             13299..13496
                     /locus_tag="IWO17_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290647.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13649..14134
                     /locus_tag="IWO17_001777"
     CDS             13649..14134
                     /locus_tag="IWO17_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ0290648.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14238..14831)
                     /gene="clpP"
                     /locus_tag="IWO17_001778"
     CDS             complement(14238..14831)
                     /gene="clpP"
                     /locus_tag="IWO17_001778"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ0290649.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            15011..15538
                     /locus_tag="IWO17_001779"
     CDS             15011..15538
                     /locus_tag="IWO17_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ0290650.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15629..16366
                     /locus_tag="IWO17_001780"
     CDS             15629..16366
                     /locus_tag="IWO17_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0290651.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16370..17287
                     /gene="miaA"
                     /locus_tag="IWO17_001781"
     CDS             16370..17287
                     /gene="miaA"
                     /locus_tag="IWO17_001781"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ0290652.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17289..18518
                     /gene="hflX"
                     /locus_tag="IWO17_001782"
     CDS             17289..18518
                     /gene="hflX"
                     /locus_tag="IWO17_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ0290653.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5991 a         3208 c         4027 g         5308 t
ORIGIN      
        1 ccgcagatgc aagcttttga acaccgtttt atttgtctca cacccttttc cttcctactt
       61 gtaaccttgc tcccgtgtta gaaacatgtt acactaaata tgtgaaatta ttaacaagga
      121 gcgtcgttat gcgagaaaaa acagatacac tgccaaaagc aactgcgaaa agattacctt
      181 tatatttacg ctacttgaag atgctggatg actcaggtat aagtagaatc aaatcaaatg
      241 agttcagcga aatcacccaa attccttctg caacgatccg cagagacttc tcgcaattag
      301 gcgagctagg cagaagcgga tatggctatg atgtcccttt tcttatcgat gtattcaata
      361 atatccttaa tactaaagaa gaaaaacgaa tcgcattagt aggatatgga aatcttggca
      421 aagcattgaa acacaacaat tttagaagaa atgaaaatct aaatatcgtg tgcgtctttg
      481 ataatgatcc agcactgatc aatcgagtaa ttgatggaga aatgatctat ccgattgatc
      541 gtttcgcgga gatagccaaa gcaaaaaatg tgacagtagc aatctctacg gtaccaagta
      601 aatattcaca atctgctatt gatgaaattg tcaaaggaaa tgtaaccgct atacttaatt
      661 ttgcgcctga cagagtaact gtcccagctt atgtcaatgt tcaatacatt gatttgacaa
      721 ccgagttgca gacgcttatt tactttgatg agaattactc agaagttttt tcttaggaaa
      781 atttgagtag gcttattgat cttgcgagaa ctcgtcgtaa ttctttcaaa aattttttag
      841 tactaaatga agtggttttt tcacgaccac ttgttgacta aaaaaaaaaa ccattgtaga
      901 atgaagcata acgctgaaca agaggagtaa ctggcacatt cattcttaga gagagaacta
      961 tctgctgaaa ggttctcaat gacaaccagt gaaggtagct tgggagtttc tgtcttgaat
     1021 ggagtagaga tagacgagtc gtgatcgtta actcacttga gaggcaataa tgattgatta
     1081 ttgcaattta ggtggtaccg cgtaaatcat acgtcctaga atacgaaagt attttaggac
     1141 gttttttagt ttaaaggagg aaaacagcat gacggaagaa aataacctat caacaaaata
     1201 taatccgcaa gaagttgaag ctggacgtta tcagcattgg ttagaacaag atttgtttaa
     1261 accgagcgga gataaaaaag caaaacctta ttcaatcgta atcccgcccc caaatgttac
     1321 tggaaaactt catttagggc atgcttggga tacgaccttg caagatatgc tgattcgtca
     1381 aaagagaatg caaggatatg atactttatg gctgcctggt atggaccatg caggaatcgc
     1441 gacacaagca aaagtagaag cgaagcttag agagcaaggg atttctcgtt atgatctagg
     1501 tcgtgaaaag ttcatcgatc aagtgtggga atggaaagaa gagtatgcga gccacatccg
     1561 cgaacagtgg gctaaaatgg gcttatcttt agattacagt agggaacgtt ttacattaga
     1621 tgatggatta tctgaagcag tacgtaaagt ttttgttacc ttatacgaaa aaggtttgat
     1681 ttatcgtggt gaatatatca tcaactggga tcctcaagca cgaacagctc tatctgatat
     1741 cgaagttatt cataaagata tcgaaggcgc cttttatcat atgagctatg aactagcaga
     1801 tggatcaggc gtagtagaaa ttgcgacaac ccgtccagaa acgatgttgg gagatacggc
     1861 gattgctgtt catccagaag acgagcgtta tcaagcgctc atcggcaaaa aagtgattct
     1921 acctttagtc aataaacaaa tcccaatcat tgcagatgaa tatgtagata tggaattcgg
     1981 gacaggtgta gtaaaaatca ctccagcaca tgatccaaac gactttgagg taggtaatcg
     2041 tcatgatctt ccacgcgtaa atgttatgga tgatgatggt acgatgaatg aactagcagg
     2101 aaaatatgct ggaatggaac gttttgctgc tcgtaaagca atcgtgcaag atctgaaaga
     2161 aatcggtcga ttgattaaaa tcgatacgat gatccacagt gtgggtcatt ccgagcggac
     2221 aggtgtcgtt gttgaacctc ggctgtctac tcaatggttt gtaaaaatgg cgccgttagc
     2281 gaaaaaagcc attgataacc aagaaacaga tcaagcagtg gaattcttcc ctccaagatt
     2341 caatcaaacg tttttacgtt ggatggaaaa tgtccatgat tgggtaatct cacgacaatt
     2401 atggtgggga catcaaattc ctgcatggta tcacaaagaa acaggtgaaa tgtatgtagg
     2461 gatggaagca ccagcggata gtgaaaactg ggtacaagat cctgatgttt tagacacttg
     2521 gtttagttca gctttgtggc ctttctcaac aatgggctgg ccagatacgg aaagcgaaga
     2581 ttataagcga tacttcccta ctagcacttt agtaaccggt tatgacatca ttttcttctg
     2641 ggtaagcaga atgattttcc aaagcttgga atttactggc gaacgtccat ttgagaatgt
     2701 cttgattcat ggattgattc gtgacgaaca agggcggaag atgagtaaat cattaggtaa
     2761 tggaatcgat ccgatggaag taatcgaaaa gtatggagca gatgctctcc gctggttctt
     2821 atcgaatggt tctgcaccag gacaagatgt ccgcttcagc tatgaaaaaa tggatgcttc
     2881 ttggaacttt atcaacaaaa tctggaatgc ttcacgtttc gttatcatga atatcgaagg
     2941 aatgaccgta gaagagatcg atttatctgg agaaaaatca gtggctgacc ggtggatcct
     3001 tactcgtttg aatgaaacga tcgaacgagt aactgagcta ttttatcgtt ttgaatttgg
     3061 tgaagcaggt cgacaactgt ataactttat ctgggatgat ttttgtgact ggtacatcga
     3121 aatgagtaaa gaagtacttt atggagatga tgctgtttca aaacaaacca cccgtagcat
     3181 cttagtccat acacttgatc agatcctacg tttattgcat ccaatcatgc cattcgttac
     3241 agaagaaata tgggaacata ttcctcatca gggaaattct ctagtagtgg cagaatatcc
     3301 tgttgtacgt ccagagtttg atgatgaaac agcttctaaa gggatggaag tattgaaaga
     3361 attgattcga gccgttcgaa atattcgttc agaagtgaat acaccattgt ctaaaccaat
     3421 tacgttgcta attaaaacaa acgatgctaa aatcgatcaa tttttagttg aaaatacaag
     3481 ctatatcgaa cgcttctgta atcccgaaga gttgacgata tcaagtgaga tcactgcgcc
     3541 agatctagcg atgtcagctg ttctgacagg tgcagaaatc ttcttgccgt tagcaggatt
     3601 gatcaatatt gaagaagaaa tcaaacgatt agaaaaagaa ctggaaaagt ggacaagcga
     3661 agtaaaacgt gtccaaggaa aactttctaa tgaacgtttt gtttctaacg cgccggaaga
     3721 agtagtagag gcagagcgtg caaaagaaaa agattattta gagaagcaaa cggctgtcaa
     3781 agaacgaatc gaaagtttgc gtactattca ataagaatag aaaaagagag tgtgaaatca
     3841 gctagctatt actaaaagct agctgattga acatcgttac aggaaagagt ttgagcctca
     3901 ctcttagctt cgattcataa ggagatgtga cataaatttt gtcgcatctc ttttttagtc
     3961 tactcttatt atgtaggaga aggagggagg tggatcatgt atcaagagtc aataaagtag
     4021 ttgataaaat cgttcctctc tctgtttctt ttctctttgt catactttta tttttcttat
     4081 gtgttctatt tgttataata aaagagaaat gggggaattg ttgatgacga ttgaagaagc
     4141 aatagaatgg atccatagtc gattaccatt tggttcacgt cctggccttg accgagtcga
     4201 agctttatta gaagtggtag ggcatccaga gagaaaagta aaaacgatcc atatcgctgg
     4261 aacaaacgga aaaggttcta ctgtagctta tttgagaagc ctattagaag aggctgggct
     4321 gacagtaggt acatttactt caccttatat cgaaacgttc aatgaacgga tcgcaatcaa
     4381 tggcaaaagc attccagaca atgatttgat ccagcttgta aaaaaatatc agccactgat
     4441 caaacaactt gatcaatttg aagcagtagc tggtattact gaattcgaga cattaacagc
     4501 aatggcattt gattattttt tgatgaagaa agtggacgta gcgattatcg aagttggctt
     4561 aggcggctta ttggattcta ccaatgtagc tgtaccaatg ctgacgggaa taacaacgat
     4621 tggacttgat cataccgaaa tacttggaga tacaattgaa gaaatcgctg cacaaaaagc
     4681 tggaatcatc aaacaaggcg ttccagtagt aacaggaaat attgccccag aagcttttac
     4741 tgtcatccaa caaacagcta aagcaaaaaa tgcaccgctt tatgtatacg gggaaaatta
     4801 taaaataaac tatctgcatc ctgatgagat gtggggagaa gtatttgaat tttttaatga
     4861 aataggaaag atccctcaat taaccactcc gcttttaggg caacatcaag ctgaaaatgc
     4921 aggagtagcc atcgaattgt attttgttta ctgtcaattg gaaaagctgc ctttccgaga
     4981 aaaagatgtt ttgaagggat tgaagaaatc tcgatggcca ggaagaatgg agcgtttgag
     5041 taacgagcca ttagttgttt tagatggtgc ccataatacg catgcaatga accggcttgt
     5101 tgagaatttg aaaaaagaat tcaaaggata tcaaatcaac attttgtttt ccgccttacg
     5161 aactaaaaat gttagagaaa tgctagaacg attgcagcaa gtaccaaatg tccatattta
     5221 tttgacgacc tttgaacatc caaaagccat cgaattaagt gaagtatcag atttagcaag
     5281 tgagagagta tcaatcgttt cattgtggca attcggatta gcagatatat tggaaaagat
     5341 gacaaatgaa gacgtattag ttgtgaccgg ttcactttat ttcatttcag aagttcgcca
     5401 tttattaata caattaggag gagatcatga agtataatgc agtgattttt gacatggatg
     5461 gtttgctgtt cgacacagag ttagtgtatt acgaagccac acaagtagta gcagacaaga
     5521 tgggttttcc ttatgataaa gacctgtatt taaaattctt aggtgtttca gatgaagaag
     5581 tatgggcgaa ttatcatatg attttctcgg aatttggcaa aacaagtgta caaagattca
     5641 tagatgattc ataccaggaa acgcttgaac gtttttcgca aggagcggta caaatgaagc
     5701 ccggtgtgtt ggaactcctc tcttttttag aagaacatca gattcccaaa gttgtcgctt
     5761 ccagtaacca acgagcagtc attgaacttt tattgaagaa gaacaatctg tttgaacgat
     5821 ttgagacgat tgtttctgca gaaaatgttc aacgagcaaa accagaccca gaaattttct
     5881 tgttagctaa tcagaagcta ggtacagcga agaaagaaac attgatttta gaagattcac
     5941 aaaatggtat tttagcagct tctgcggctg agatcccggt agtcatggta ccagatctgc
     6001 ttccaccatc ccaagaattg atcaaaaaaa cagctggggt tgtttcttct ttacatgaaa
     6061 tccctgcatt gttaaaataa aaatccatct cttttcgtat cttattttag ataagataga
     6121 aaggagatgt ttttttggga aaacgattga tcaaagaagt acctactagc tctttaccaa
     6181 gggaacgtat ggaaatttat ggcgcggaag ctttatcgga tcaggagctt ttggcaatct
     6241 tactgcgtac cggtcaacac ccttatagtg tgatgtcgat tgctggaaat ctgttaaaaa
     6301 cgtttggagg actggcctct ttacgacagg ctactttgca tgagttagaa gagattcgag
     6361 ggataggacg cgtgaaagca atcgaaatca aagccttgat cgaattaggt cgacgaattc
     6421 agacggatca tgaaagagcg gctccaccgg ttcgtaccag ctacgaattg gcccagcaat
     6481 tgattctgga aatgaaagat cacaaacaag aacatttggt ttgtatttat ttggacacga
     6541 agaatcaagt gatcttaaaa aaaacagtgt tcatcggttc gctgaatcag tcagtcgctc
     6601 atcctagaga aattttccat tatgctgtac gttattgtgc agcaaggatc gttttagcgc
     6661 ataatcatcc ttctggtaat gttattccat cgcagcagga tatgaatttt accaaaagga
     6721 tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca
     6781 aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc
     6841 aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa
     6901 catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca
     6961 cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt
     7021 tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg
     7081 tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga
     7141 ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat
     7201 ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga
     7261 caatttctct tgtggcaggt atttttttgt atgaaaaagt aaataaattt aagaggctat
     7321 gaaagagcca ccttgtatca aggaataaga acaaacaaaa atggcttctc atgtttttca
     7381 tttgttttct cttatattca ggacaaggtg ctgatttcat aacctcttat ttattcttca
     7441 tctggtgtca ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa
     7501 ggctgaagcg cgccagtcat cgcttctttc aatttatatt ctgaacagtc aggtccattc
     7561 aaacattctc ttaaagcgtg gaataagatt cgctgagctt cattgatcat ttctcctgat
     7621 tctcgcatgt agacaaatcc acgagagaga atatctggac cagccaagat ttcttttttc
     7681 ttgatatcca ctgtcgcgac agctaataca agaccttctt ctgaaagcag gtgacggtca
     7741 cgtaaaacga cattaccaat atcaccgatc ccgctaccat cgatatacac atctccagcc
     7801 acaaaatgcc cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc gccattttcc
     7861 atgatgaagc agttttctgc aggaacacct acatcttgtg ctagagaagc atgtactttc
     7921 aacatgcgga attctccgtg gacaggaata aaatattttg gcttcatcaa acgaagcatt
     7981 aatttttgtt cttcttgtcc accgtgaccg gaagtatgga tgttattgat ttttccgtga
     8041 atcacttctg caccagcttc agacagcaaa ttgatcaaat gattgacact tgttgtatta
     8101 ccaggaatag gtgaacttga aaagatcact gtatcgtctg gctggatttg gatttgacgg
     8161 tgagtaccat tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga acctgtacat
     8221 aggatcatcg tttcattggc aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg
     8281 tctggaactt tgatatatcc taaacgttga ccgttgacga tggcattttc catgcttcgt
     8341 ccgaaaacag cgattttacg accagttgcc acagcggcat ctgctgcttg ctgcaaacgg
     8401 aagatgttag aagcaaagct ggcaaaaatg atacgtcctt cgatttttga aaagatcttc
     8461 aaaatagaat gcccaatcgt tttttctgat ttagtaaatg ttggaatctc agcatttgta
     8521 ctatcagata aaaggcacaa gactccttct tccccgatac gagccatttt atgcaagttt
     8581 gctggttcgc caacaggagt gaaatcgaat ttgaagtctc cagtcgctac gatatttcca
     8641 gaaggtgttt tcaccacgat ccctaatgca tctgggatac tatgggtcgt tctgaaaaag
     8701 ctgacagaag tctttctaaa acggatgatc gtatcttcat tgatctcatg gagctctgca
     8761 tctcgaagca agccatgctc atctaatttg tttgtgatca acgctaatgc gagaggacca
     8821 gcatagatag gaatatttgc ttgtttcagc aagtagggta ttccgccgat gtggtcttcg
     8881 tgcccgtgag taatcaccaa ggcttttact ttgtgtaagt tttgtatgat gtagctgtag
     8941 tcgggaatga catagtcaat tccaagtaaa tcgtcttccg ggaatttgat cccggcatca
     9001 atcaaaatga tttcatcttg gaattgtacc ccatacgtat ttttcccgat ttcacctaat
     9061 ccaccaatag caaagacgcc tgtctcgtta tttttgatgg aaagtttcat attaaaactc
     9121 cactaatgag aaatcagcat tttctttttc gtactctaga tgacttcctt ctagaagttg
     9181 aacgaattca atattgtatg gggtattttc ttccacacgt tggcgagcgt ctacatcgct
     9241 ttttgcctca atgtaaagag attttgtttc ttcccgttta gggttgcgta cttttgtttc
     9301 ttgataataa actttataga tcattgtttt gtctccttta tctgcgtaaa gcagtgattt
     9361 agctagttga tttttttaga caacacaaaa cgaggggaca agccacctgg tttttcatat
     9421 gctttgacta gaatactgga ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt
     9481 cggatatttt ttcaaccgta ccttctgtat aaaaaatata caacaattaa ttgttattct
     9541 agcataaaac agcaaataag tatagaaatc aactagattg aaagaaaaaa tcaatgttta
     9601 tcatcccgct ataatcataa tgactttttt atgagagaag tgttctatta ataggctcgt
     9661 tgtgctatca tttttagtaa tgaaaatgta acaaaagatt aaaaggagat gagaaagtga
     9721 aactaatgtg gcattatacc atgcgttata agaaattctt gtttttcaat ttcatatgcg
     9781 tatttggttt tattttgatt gaattaggac ttcctacgat tttagcacgt atgatcgatg
     9841 taggaatccg caatgatgat tttgattatg tgaagcaaca agggatattt atgatcgtta
     9901 ttactgtaat cggtattatt ttgaatatct tgttaggtta ttttggttcg aggattacga
     9961 ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc
    10021 aggaatacga aaatctaggc gtatcttcat tgatcactcg tacaacaaac gatgcttatc
    10081 agatcatgtt gttcctgcaa aatattttac gtatcggttt tatggcacca atgatgttca
    10141 ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg
    10201 cattaccttt actattattg gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta
    10261 aaaagcaaca aaaaaactta gataaaatca acagtatctt gcgggaaaat ctttcaggat
    10321 tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca
    10381 atgagaatta tgcttctagt tcaaaaagct tattccgttt gatggctgtt gcacaaccgg
    10441 gtttttattt cttatttaat atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa
    10501 tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat atattccatg
    10561 ccttgttctc attcatgtta tttgctagtg tgttcatgat gtatccaaga gcagctgttt
    10621 cagctggacg tatccaagaa gccctagatg ccaaaccttc gattgtagaa gatccaaatg
    10681 gtatatctga tacaaaaact aaagggtata tcgaatttca aaatgtaaca tttgcttatc
    10741 caggccattc gcaagagcca gtgatacgaa atgtcagttt tacagcctct ccaggagaga
    10801 ccgttgcttt tattggaagt accggtagcg gaaaatctac gttgatccaa ttgatccctc
    10861 gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg agagattatc
    10921 ggctaagcaa gttgcgagat aagattgggt ttattccgca aaaagcgctg ctatttactg
    10981 gaacgattgc agataatttg cgatacggga aagaagatgc tacgcaagaa gaaatggaac
    11041 gtgcagctga gattgctcaa gcttcagaat tcatttctcg aaaaccagat ggatttaatg
    11101 aacatttatc agaaggtggt tccaattttt caggtggaca aaaacaacga ttggcaatag
    11161 ccagagccat catacgccgt ccggaaatct atatttttga tgacagcttt tctgcattag
    11221 attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg
    11281 tattgatcgt agcacaacgt gtgggaacga tcatgcatgc agataagatc atcgtattaa
    11341 atgaaggaga agtcgtcggt atggggacgc atcgggagtt gttggaaaaa tgcccgattt
    11401 attatgatat tgccgcttct caattatcaa aggaggaatt agcatgaaaa aaaccttttc
    11461 ttccttaaaa cgtttagctc gatatatcag accgtatcgt gagacttttc ttttagtgat
    11521 cctatttaca gttctgacag tggctttcaa cgtggctttg ccatatgtta caggacttcc
    11581 tactacagaa atcagccgta atattgccaa tggcgaatca atcaattttt cttatatatt
    11641 caaatgtctc atttggatca cagttgttgg aatagggtat tgtatttcgc aattactttc
    11701 tggtgttttg atgacaaatg tcgttcaaag tgccatgcat gatttgagaa gagacataga
    11761 agaaaaaatc aatcgtcttc cggtttcata tttcgataaa aaccagcaag gaaatatttt
    11821 gtctcgtgtg acgaatgatg tagatgcagt cagcggtgct ttacaacaag cttttattgg
    11881 tatcgtaaat gcgatattgg gaattacgtt ggctgcagca atgatgtttt atatccagcc
    11941 gatgatggcg ctgatctcta tgatcatgat tccaacatcg atctggatct caaaacgagt
    12001 gatcaatgct tcgcagaaat attttcaaag catgcagaat tctcttggtg agctaaacgg
    12061 ctacgtccaa gaaaatatga caggttttag tgtattgaaa gtatatggac gcgaaaaaga
    12121 aacattggaa ggattcaaaa aagttaatca cagcttgaaa tactttgggt ttcgtgcagc
    12181 attcatttct ggcttgatga tgcctctagt tcaattaact gcctatgcta cttatattgg
    12241 tatggctgtt ctaggaagct tttatgttat tactggggcg atcgttgtcg gtcaactaca
    12301 agcattcatc caatatattt ggcaaatcag tcagccaatg gggaacgtga cacaattgtc
    12361 ttcagttttg caaagtgcat cagcggcaac gaaacgtgta ttcgagattt tagatgagcc
    12421 agaagaaaaa gaaaacaaag tagatgcttc actgccagaa acgattttag gagatgtgac
    12481 attcgatcat gtcgattttg catatgatcc gaagaaacca ttaatccaag atttgaattt
    12541 tgaagtgaaa gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg gcaagacgac
    12601 attgatcaat ctattgatgc gtttttatga cgtagacaaa ggagcaataa ggattgatgg
    12661 aatcgacacc aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca
    12721 agatgcatgg ctttatcaag gaacgattgc agataatatc cgcttcggaa agcttgatgc
    12781 gacagattat gaagtagttg atgctgcgaa aacagcaaac gtagatcatt ttatccgaac
    12841 gatgccagat ggatatgata tggccatcaa ttctgaggga gacaatgtgt ctttaggaca
    12901 aaaacagttg ctgacgattg cccgagcagt ggtctccgat cctaaaatct tgattttaga
    12961 tgaagcaaca agttcagtag acacccgttt agaagcgttg attcaaaaag caatggatcg
    13021 cgtgatggag ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc
    13081 agatctgatt ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc atgagagttt
    13141 attggcacaa ggtggatttt atgaaaaact gtataacagc caatttgctg aggaagcaga
    13201 ataagtcata taagaataaa acagtttaaa acttattttt tgttctctat tcttgataat
    13261 ggtaaagtaa agttatcaag aaaagtgagg acacggagat ggaacagttt ttaaatgaaa
    13321 aaattactga agccaaaaga gaagttgatc gtttaacgca cgtctgtcag acgaagcctt
    13381 ttgattcagc tgattttttt gaaaatgagt ttcaaatcga acgcttggca gcacaaatag
    13441 aagtgtacga attaatttta aaaaaagtaa tagaagaaaa tatagatgta caataaaaac
    13501 aggtaaaacg tcagttacca gatactgtaa atagacatct ggcctgagca ggttttacct
    13561 gttttttttc ttatgaaata aatatatgct atgatacctt tattcaatag gattagtttc
    13621 tacaaaaaat gaaaggagaa aaataaatat ggaacggttt atagcatcaa atgccacagt
    13681 gattggtgat gtgacattgt cagaagatgt gacgatctgg tatcaagctg ttttacgcgg
    13741 ggacagtaat tggataaaaa taggacaaag aacgaatatc caagatggta caatcatcca
    13801 tgtcgatcat gatgcacctg tagatattgc ggagaatgtg acggtaggac atcagtgcat
    13861 gctacatggc tgtacaatcg aaaaaggggc gctgatcggg atggggacca cgatattgaa
    13921 tcatgcggtt attggtgaga atagtttgat tggtgctgga tcattagtaa cagaaggaaa
    13981 agttattcct cctaatgtct tggcttttgg tcgcccagct aaagtgatcc gtcctttgac
    14041 aaaagaagaa atacaaaaaa acaaagaaaa catccagcat tatgtcgaga taggtcggat
    14101 gcatgcgcaa ggaaaattca aggagcaaag ataggcagta gcatccataa aataaaaaag
    14161 gatggtcagt ttgagttgac catccttttt tatttggttt agataagaga gcttaaaaaa
    14221 gcttcctttg aaacagatta atttaatgaa gaactgtttt ccatgacttc atcgatcaat
    14281 ccatatgctt ttgcttgttc agctgtcata tagttgtcac gatctgtatc tttttcaatc
    14341 acttcaagcg gttgtccagt gcgttcagct aaaattttgt ttaagcgttc gcgtgtttgc
    14401 aaaatatggc gtgccgcaat ttcgatttca gttgcttgcc cttgagcgcc gccaagaggt
    14461 tgatggatca tgatctcagc atttggtaaa gcaaatcgtt taccttttgt accagctgtc
    14521 agcaagaagc ttcccattga agcagccata cccataacaa ttgtttggac atctgctttg
    14581 acaaagttca ttgtgtcgta aatagccata cctgctgtaa ctgaaccacc aggagaattg
    14641 atataaagat agatatcttt ttcagaatct tgagcgtcta agaaaagcaa ttgggcgatg
    14701 atcgaattag caagatcatc tgttacttgg ccgcttaaca tgatgatacg gtctttcaat
    14761 aaacgtgagt agatatcgta agctctttca cctcgtgaag attgttcaat gactgtagga
    14821 attaaattca taatagagtt cctccttgat ttcgtttaga aatagtgtac cataatttag
    14881 tttctaatac ctaaaatgta gtatacattt ttggtcaaaa aaggtcaaat aaaaaactta
    14941 ggcaaattag tcgcataaac ccgctttatg gtatgataag cagagaaaca aaacaaggag
    15001 gaacaacaga ttgatagttg gtattactgg ccttatcgga gtgctgacag gtgctgtagt
    15061 tgtctatatc ggcatgatac tgcaaatgcg tcacttggaa aataaagaac agcgtagacg
    15121 tgaattggaa ttgaaagtca aagaaatcga gacgctgaat caattaaata aaaagatcaa
    15181 cgagatcttg caaaaacgga caacgctgat ggaagaatat gtcagctttg atgcatttga
    15241 tgattgttat atcacgattg acgattttgc gtatctccaa tcattttcag cgcaaaatag
    15301 tttttatctg ccaaattata ttttagacga gctatttaag aatatctccc atcgaaaagt
    15361 gattttatct ccagatgaaa caatgaagat tggtggatat gcttataaag gcggacgcgt
    15421 gattttagag aattttacgg atagtctgac tgaaatgatc aatgaaaaaa aagctcagtt
    15481 gaagactatg acgcgccagc cgattggttt cttctcgaaa gatgatgttt ggagctgata
    15541 ttttgaagca ttgccgttgc acatgagagc gtggtaatgc tttctttttt tcttaatgat
    15601 acaatagaac tgaacggagt gaagaaagat gttacaaatc tatgcccatc gcggaagcag
    15661 cggcacccac ccagaaaata cgcttcctgc atttgcagaa gctgtacgag taggtgcaga
    15721 tggtatcgaa ctagatgtcc atctaagcaa agatggttat ttgatcgtta tgcacgacga
    15781 agaagtggac cggacgacta acggtaaagg gctgattcga gaaaaaacat tggaagaact
    15841 caaaaaattg aatgcagggg gcaaattcag caaagaattt ccagcagcta aagttcctga
    15901 attgagagaa gtgttaggac tgctcctcca aaaaaattac cgagggatgc taatgattga
    15961 aattaaaact gatcagtatg aatatcccgg tatcgaagaa aaattagccg aaacattaaa
    16021 aagtagagat tggccttttc aacatgcata ttgcagcttt catttggatt ctttggaaaa
    16081 aatcgcatta ctggaaccgg agatacagct ggacttattg atgagtactt ctgataaaaa
    16141 gatccaacta gcagcagaaa ccccttatat agaaggagta catccaaaaa atacttggtt
    16201 gttcgaacat ccagtccagg cagctgattt tccaaaagca attcgtgcat ggacgattaa
    16261 cgatgaagaa gaaatggcca aatgtcaaga actcaaaata gatggaataa taactgattt
    16321 tccggagaaa gctcttcggt ggaaacaatc aacgaaaagt aggtaaaata tggaaaaagt
    16381 attagtgatc gtcggtccga cagcagtcgg taaaacagcg ctaagtgtgg aactcgcaca
    16441 actatttgga ggagagatca tcagtggtga ttcattgcaa atctataaaa atctggatat
    16501 cggtacagca aaaatcaaag aagaagagat gaataatgtt ccgcatcatt tgattgatgt
    16561 gatcgaacct actcaacgtt actcagccgc tgattttcag cattcaggtc gacaattgat
    16621 taccgagatt gcgaaccgag ggcacctacc tatcatagct ggtggtacgg gtctctatat
    16681 ccaatccctt ctatatgatt atcagttagg agcgacagaa gaagcgaatc ctgagattag
    16741 aaaaaagtat gaaacgttag ctgaaacaat tggttcccaa gcattatgga atcagttgaa
    16801 agaaaaagac ccggcagctg ctgagaaaat ccactggaac aatcaacgca aagtgataag
    16861 agctctggaa gttttagaga cgacaggaca tagtatcacg gcacctaaag aagagccaaa
    16921 gcaattgtat gattacctca tgattggatt agatacgaag cgtagtattc tttatgaacg
    16981 aatcaatcaa agagtcgatt tgatgctagc agaaggttta gtagcagaag cgcgtaaggt
    17041 gtatgagctt ggagaagtcc aggccagtca aggtattgga tataaagaat ttttccctta
    17101 ttttgaaggg aaagaaacat tggaagaagc aacagaacaa gtcaaactac attcgagaag
    17161 atacgcgaaa cgtcagttga cgtggtttcg taaccgtttg gatgcacagt ggtttgatct
    17221 tataagtgag ccaaatcaaa aaagtcagat tgagcaaaca atcaaaaaat ggctggaggc
    17281 ggaataaaat ggaagaaaaa gtgatcatcg taggggtaga aaccgaagca aaccagcgtt
    17341 acttttcaga gtcaatggaa gagctagttc agctgacgaa taccgcatca ggagaagtcg
    17401 tattcacaat tacacaaaag agaccacaag tggatcggca aacgattatc ggtaaaggga
    17461 aattacaaga attagttcaa caagcggatg cgcatgaagc ggatttgatc atctttaatc
    17521 atgagctaac tccgagacag agccaactga ttaccgatgc tgttggtctt ccggtaattg
    17581 atcgcgtgca gcttatctta gatatttttg cgatgcgtgc tcgttcgaaa gaagggaagc
    17641 tgcaggttga acttgctcag ttagaatacc tgctgcctcg tttagtaggg caagggaaaa
    17701 cattatctcg tcttggcggt gggatcggaa caagagggcc aggtgaaaca aagttagaaa
    17761 cagaccgccg acatatacgg aataaaattt ttgctgttaa gaaagaattg aaagaagtag
    17821 aagctcatcg agagcgcaat cgccaaaaaa gaaaaaatag cgagattttt cagattggac
    17881 ttattggcta cacgaatgcc gggaagtcaa ctatcttgaa tctattgaca caagcggata
    17941 cttattcgaa agatcaattg tttgcaacgt tagatccatt gactaagaga tggcgttttg
    18001 ctgaaggatt tgagattact gtaacggata ccgtcgggtt catccaagac ctgcctacgc
    18061 aactgatcga tgctttccat tctacattag aagaaagtca aaatatggat cttttgctac
    18121 atgtagtcga tgctagttct ccagatcgta ttttacagga gcagactgtc ttaaaattga
    18181 tggatgaact gaatatgaaa gaaatgccga tactgactgt ttacaataag gcagaccaga
    18241 tcgacccagc gatgttcacg cctacgctat tcccaaatgt attgatttct actcagacga
    18301 aagaaggtaa gatggcgttg atcgaagcaa tcaaacgtca gctgatggaa ctaatgaccc
    18361 cttatacact ttctgtgcca agcaacgaag gacagaaatt aagcgagttg agaagacaaa
    18421 caatggtttt ggacgaagcg tacatggaag aaacaaacga atatgaagtt aaagggtttg
    18481 caaaaaccaa ttcaaaatgg ttaagaaaaa accaataaaa ttttattttt ttaa
//
LOCUS       DACECR010000057        18285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.057, whole genome shotgun sequence.
ACCESSION   DACECR010000057 DACECR010000000
VERSION     DACECR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.057"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..2177)
                     /locus_tag="IWO17_001783"
     CDS             complement(<1..2177)
                     /locus_tag="IWO17_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290654.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="IWO17_001784"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="IWO17_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            complement(4085..4846)
                     /locus_tag="IWO17_001785"
     CDS             complement(4085..4846)
                     /locus_tag="IWO17_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0290655.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            4946..6667
                     /locus_tag="IWO17_001786"
     CDS             4946..6667
                     /locus_tag="IWO17_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0290656.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            6682..8466
                     /locus_tag="IWO17_001787"
     CDS             6682..8466
                     /locus_tag="IWO17_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0290657.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8847..10400)
                     /locus_tag="IWO17_001788"
     CDS             complement(8847..10400)
                     /locus_tag="IWO17_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0290658.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10748..13162)
                     /locus_tag="IWO17_001789"
     CDS             complement(10748..13162)
                     /locus_tag="IWO17_001789"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ0290659.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            complement(13589..15607)
                     /locus_tag="IWO17_001790"
     CDS             complement(13589..15607)
                     /locus_tag="IWO17_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0290660.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            15977..16633
                     /locus_tag="IWO17_001791"
     CDS             15977..16633
                     /locus_tag="IWO17_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0290661.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            16633..17589
                     /locus_tag="IWO17_001792"
     CDS             16633..17589
                     /locus_tag="IWO17_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ0290662.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            17589..18155
                     /locus_tag="IWO17_001793"
     CDS             17589..18155
                     /locus_tag="IWO17_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0290663.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
BASE COUNT         5329 a         3697 c         3262 g         5997 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acatttttat aaaacgacga aaaataaatc actaccagtc aacttctata
     3421 atattaccaa ataacaaact aactatccat caataacgtg ttttattttt taaaatttct
     3481 ataaaaaaaa caatcattct taaaaagaat atataaaaat aacaataacc tttgtgattt
     3541 ttcttgtttg aagaatgtta gaagaaaaat ctctaaatac ttataattat tttataagca
     3601 aaaaaatgct atcggtagta tcaccgatag ctgaggaaag tcacagtagg gactatttga
     3661 ttaaaaaata tgtttattca atccatctat ttatttttgc cttattaaca tattgtaata
     3721 atgtaagcaa caatgtattt taagtgtaat gcttttatgc acctatttaa tttatcattc
     3781 tcatctgata caatctcact cataacaata tgaatttttc ttctcattag tctcagttca
     3841 ccatgaatta tacataacac ccaatacaat tatttttcca tactttttta agaggacaat
     3901 tacatcgtat atatcgctaa gaattgcaac cattccattt ttaaagacat atttcctcta
     3961 aaaaactaaa attctatcta tccacgacta ataatccccc acattaaacc cgtaatcgaa
     4021 ccagaaaatt tttctttata actaaaacaa ccatcatttt cgagttatct taaacacata
     4081 tttcctattt tttcatttct atccatgcat gataatattc agttctgata ttgttttcat
     4141 ccgtattcgt caatagatac tggccgatca cttctcctgt tttccatcct ttttcataca
     4201 ttctttctct gatttctaaa atattattac gttccgcatg gttcatctcg gatttcaata
     4261 agaatcgacg ataaacagcc gtatcttttt tctcccaaat cacgtcttga gtaaataaat
     4321 gtacttcact agggtcgata aaaaatccat acatcgtctg tgaactggaa gctggcatac
     4381 tcatcatccc aaagcttgaa tagttaggta gaaatttttt gatttccaca atatcttcaa
     4441 actccgttga gatcatacag tcaaaaggga tctcttctac aggaaattct tcctcgtttg
     4501 tatcgatcat caatttcaac tgtttttttt gctcccttat ctctttttgt ttttgtttca
     4561 tcaccaccag ttctttatgg attcttcttt ctgtttcatc aagtgtctca tataactccg
     4621 tcaatgtttt tccatataaa ttcttcatct gtttgatcgg tatatctaat tttctataaa
     4681 aaacgatatc ataaagcata aaaatatcat cgatatcaaa caatcgataa tcattctctt
     4741 cttgtctact tgaacggatt aatccttgtt catcccaata gcggatcgtt gattttggca
     4801 aatcaaatag ttccgatact tggccaatcg tatacatttt tttcattttt tctcacctct
     4861 ttttcagttt attctttttt ctattgacct tcaagttact tgaaggtata gaatcgaaaa
     4921 gtatcaggaa aaaaggagga ataatatgtc tatcttaaaa acattcttac agaaaaacaa
     4981 aggactcgct ctccttacct ttgtgatgat ctgtttagaa atcgccggta cattaggtgt
     5041 accaaaatta gtagcaaagc tgatcgacac aggtatagca agtggcgaag cctcagttat
     5101 aaaaaaaatt ggagttgaaa tgctgcttgt agcgtttcta ggaacaatcg ctgcagttgc
     5161 ctctagttac ttttcagcat tggcttccgc taggtttgga ttccaaacgc gggagaaata
     5221 tttccgtaaa ttccaacagc tgtcaatgaa ggatatcagt cgttttagtt ctggatctct
     5281 tttgacaaga atgacgaatg atgtcgataa tgtccagcaa atgattgttt tattttgcca
     5341 aatgatctta ccagcaccgg ttatttgcgt tttcaccatt ctcatgatgt ttcgttattc
     5401 tctattacta acatgggtta cattgatctc tgttgttttt tatgtatgga ttgtttatcg
     5461 actgatgaaa cgaggggcac ctttatcttt gagtatccag ccaaaaatgg atcggatcac
     5521 catcacattg agagaattct ttactgggat taatatgatc cgtgcctttg ataatcaatc
     5581 atatgaagaa aagcgaacga ataagagctt tgctgactat gctaatcaaa tgatcaaagt
     5641 gaaccgaata tttgcttggg tcactccaat tgcttttttg ctcatgggaa tcgtttatgc
     5701 ttctattttg tggtttggcg gaaatcttgt agcagaagga gcattacaaa tcggtgtcgt
     5761 gactgctgta gtcgaatact ctatgctgac acttgcttac ttgatgatcg cagctatggt
     5821 attggtcatt atccctaaat cgtttgcctc attacgacgg atagaagaaa tactgcaagc
     5881 agagatcgaa attgaggaag agacgatcaa aacagtagct gaaccaacct ttgatcagac
     5941 aaatgcagct gcttttgaac atgtcacatt taaatatact gaaatctctg atccagttct
     6001 agaagatatt gatttcacaa tacctaaagg aaaaacaaca gcgatcgttg gcgggaccgg
     6061 atctggaaaa agtagtttgg cgaaacttct cttgagactt agtgacccta cgataggaaa
     6121 agtaaccctt ggtggtattc ctcttactca aatgactcaa gaacagatcc gctcccacat
     6181 cagttatgtc cctcagaagg ctttcttatt cagcgggaca attttaagca acttgcagat
     6241 gggaaatgaa tatgcctcaa cagaagagtt gtctaaagcg atcaagatcg ctcaattaga
     6301 agaactagtg gacacccttc ctgacggatt ggacagcttt gttgctcaag gcggttctaa
     6361 ttattcaggg ggccagaaac agcggatatg tattgctcga gcgttgatca aaccagcaga
     6421 gatctatgta ttcgatgata gtttctccgc tttagactat aaaacagatg cagcattaag
     6481 agcagccctt cataaacaca tgtcggagaa aactctcgtg attgttgctc aacgcctcag
     6541 tacaatccaa caagcagata acatcattgt tttagatgaa ggaaaaatcg ttggacaagg
     6601 tactcatgaa gaactactgt ctaattgcca aacttatcag gagtttgcaa aatcacaagg
     6661 aatatagaaa gaagggatac gatggaaaag aaaaaaacgt tgaacacgcg tgctttgata
     6721 cgtttttgga aaatgatcaa acctgaacat aaatattttt acggattgtt tctatgcagt
     6781 ttattcggga acctattagt cgtagccatg cccttgatca tgggaatcgg gatcgatgat
     6841 ctcttatcag aaataagaaa aacaggtatt ggacagcttt ctttcagtga tatcaaacaa
     6901 gcattgttgt tccctgtctt attgctgatc ggctgttcag tcttaagcag catcacttca
     6961 tttattcagg aacggacaat ggcagcgtta agcgaaaaga tcacattgag ggtgcgaaaa
     7021 gaaatcacaa aaaaattcaa aacattaccg atgtcgtttt ttgacaatca tcaagtaggt
     7081 gacatcatta gtcggacaac taccggtctc aatcaacttt ctcaagtgtt attgacaggt
     7141 atcaatcaat tttttacttc tgtcgtaaca atagctgctg ctggtctgat gctgttttat
     7201 atcgaaagca agcttacttt gctcgtctta ttgctgatcg ttggcagttc aattgttaca
     7261 actaagattg cgaacaaaaa caaacagatt tctgaacaga atcagtatga actgggtgtt
     7321 ttaaataatc gaatggaaga aatcttagct ggtaatctag tggtcaaaac attcaatcag
     7381 caagctcatg cagaagctgc tatacacgaa gtaaataaaa agcaatacaa cgcttttaaa
     7441 cgtgctcaat ttatgaactg tgctatctat ccggccattc gtttcattaa tcaactcgcc
     7501 ttcatcataa gtgcggtatt cggtgctatg cttgttctat caggtggtgt gacgatcggc
     7561 ttcttacaag cttatcttca gtacatcaat cagatttctg aaccaatttc taccgcttct
     7621 tatgtgatca actcgatcca atccgcaatg gcagcaatcg ataggatatt tgagatatta
     7681 gatgaaacag atgaaattcc agaccttcca gatgcaagac ttttagaaca accgaaagga
     7741 aaaatagctt tcaaaaatgt acagtttggt tacacatcag ataaaatatt gatgaatcaa
     7801 gtcgattttt cagtaaagcc aaaacaaacg gtagcgatcg ttggtccaac tggtgcagga
     7861 aaaacgactt tagtcaatct gctgatgcgt ttttatgaac tgaataaagg aaagatcgaa
     7921 ttcgacggtc ttgatattac caagctttca cgtggagaac tacgtcgttc ttttgggatg
     7981 gttttacaaa atacctggct gtttgaagga acggtagctg ataatatcgc ctacgggaaa
     8041 atggatgcca caagagaaca aattatcgaa gcggctgaaa tcgcacaatg tgatcacttt
     8101 attcgaacac tgccagacgg atatgatacg atcatttcta gcgaaaacgg cacgttatct
     8161 caaggacagc aacaattatt gacaattgct cgagtcattc tagcgaatcc tcctgtcgtt
     8221 attttagatg aagcaacttc tagcgtagat acacgaacag aagcattgat tcagcaagca
     8281 atggaagctg tgacaaaaga taggacaagt tttgtgattg cacaccgttt gtcaacgatt
     8341 gaaaatgcag acatgatcct tgtaatgaag aatggcgata tcgtcgaaaa aggaacacat
     8401 tctgaactac tccaatcacc aacgctttat gccagtcttt ataatagtca atttcagata
     8461 gcgtaacaaa cattatctaa aaagctatcg atcatgtatg attagtccat gatcgatagc
     8521 ttttttaaat gtactagtgg tgccactaga aacacaaaga ataatactaa tgatactttt
     8581 tggatagctc cttcagtttc gttcggtaag tagtgacaaa agacttgtca caatcccttt
     8641 ttacgaataa acggtgttca agagacaacc tatcggcaat aagacacgat cttgtaaaat
     8701 acgagaagct atacttcaaa atcgcttctt gttgctcttg tcaactccga atcacgagtt
     8761 acactattca acaaatatga gaagctgttt tttgaatttg tttcttgtga ctcggtgctg
     8821 aacgtcaggc tcacaatctc tcttctttaa tctccttgtt ctacttgcga tagttgctga
     8881 acgaattgcc atgtttcccc gttatcagtt gaacggaaga ggcaggcaaa cattttccct
     8941 gcatcgattg gtcctttttt caacaaataa aattttgtct ctaacgtatt cgcgcttatt
     9001 tgagtaactt catttggtga ttgaaaaaga tctatccccc ctgtctgata gttttccgga
     9061 atcgacagaa ctgattcttt gaatgagcgt cctccgttgt ttgtataaaa caaactttca
     9121 cgtgtcgaaa caaaacctaa agaagttgta acgaagctga cattttgtac tggctgattg
     9181 atcattgtac ttccactttt tatccaagtg attccgccat ccatagtagt gtaaatgtac
     9241 aaagtttctg cagacatccc actggctgat gtacagataa atagaccatc gttgccataa
     9301 aataccgctt ttcgataacg tatcggctga actgattggg tgataataga cgtttgccat
     9361 gttttaccat tatctcttga agtcagcaga cgtacatcat ccccagaaga atagataaac
     9421 catgagaaat cggcggaaat atcatatgtg ttgtccatcc aataaccttc aggaatcagc
     9481 cccgttgtca gggtgtattc gccaaaacgc aaccattgcg aatcaatcgg cacaaatacc
     9541 caattagcac cactatctgt tgtcatatag agagagtgcg tctctgtatc aagtttgaga
     9601 tcatttatct tttgtgttgt caaatttacc ttttgcttag gatcagctaa caacccttca
     9661 tttgtctttt gtatatgcgt atcgttagtt gagttcattg tcatgtgaga agcagcattt
     9721 atatgagaag acatttttgg ctgagtgtat ctcccataat agattgttcc agctaaaaga
     9781 atgaggaaaa aaccagtgac taatgtactc atcttagttg tcaggtatcc accaggcgaa
     9841 gactggcttt tttcccatct gggttcgttt tctagtaaaa aaatatggaa tgtgttcatt
     9901 ccgaattttg atttgctttc gcctaagtag ataggtaaat catcaagtga tctcagcaat
     9961 aactggcaat cacttttttg tatccgaaaa gcttgactcg tcgaagtctt ctcaaaataa
    10021 agatagtcat catcttgaga agttggttta ttatgtaaaa actgttgtgc tctttgacta
    10081 agccattgat cgaaatgacg cgacaattga tggtaacgga gccaaataag tagaagaaaa
    10141 acaataagca caacaattgt tattccgcca actccccacg aaaagtgata caaattattt
    10201 aatttttgca tcccttcagc tgaacggact actgcactca tatggcgata taggaacgat
    10261 acagctccga aaaaaagcaa accagagaaa aatactaagc ttaaaagctt gaacattatt
    10321 cttttcgctc tttttttcgc tttctcgata attttttgtt tagctgataa tacttcgata
    10381 ataaatagtt cttgatccat ttgtctctcc ttttctcata cttcattcat tatgcgtcaa
    10441 aatccataca atcactagtc ccactaataa aaaatttatg aatttggtat gtactctcaa
    10501 tttcaggtag tttatacatc aaaaaatact aagattaata gacaaaaatt tgatgattac
    10561 agagatctct tacaagacca ttcagaagaa aaagacattg aacgtgtttg agaacatgaa
    10621 aatcaaaacc tttttgatca atcaattaat caatttgaaa taacaaaaaa tctgagacag
    10681 aatgaagttc attctggccc agatttgatt ttgcacaact catcagtcct tattgacaaa
    10741 gagccaatta attagcaacg atattgacta acttgtttgg taccgcaata acttttctga
    10801 ctgttttgcc ttcaatatat tcttgcacta tttcattggc taatgctgct ttttctagct
    10861 catcttttcc taagtctcgg ggaacattgc ttttcgcacg aactttaccg tttacttgga
    10921 aaacaacttc tacttcgtct tcaacaagtg ctgcttcatc gtatgttggc catggtgcgt
    10981 aagaaatacc accatcattt ccaaggattg cccatagttc ttcacccatg tgaggagcaa
    11041 tcggtgcgag tagttgaaca aatccttcga tatattcata aggcagagcg tccactttat
    11101 aggcttcatt gataaagacc atcaattggg agattgctgt attgaaatga agattttcat
    11161 agtcttctgt cacttttttc actgtttgat gatacacttt atcaagcttg ccatcattca
    11221 atgtagtgat acggtcacgc attttattgt tctcatcaac gatcaaacgc catacacgat
    11281 ccaagaattt acggcttcct tctaagccat tttcgctcca agcaatcgaa gcatctaatg
    11341 gccccatgaa catttcgtac atgcgcagtg tatctgcacc gtaagtttcg accacatcat
    11401 caggattgac tacattacca cgagattttg acattttttc gttgttttcg cctaagatca
    11461 ttccttgatt gtatagtttt tggaatggtt cttttgtagg aacaacaccg atatcgtata
    11521 agaatttatg ccagaatcgt gcatatagta aatgaagtac cgcatgttct gctccaccaa
    11581 tgtagatgtc aactggtagc caacgcttca acttttcata atctgctagt tcttttttgt
    11641 tatgagggtc gatataacgc aggtaatacc atgagcttcc tgcccattgt ggcattgtat
    11701 tcgtttcgcg acggcctttt ttacctgtta caggatcaac gacattgacc cactcctcga
    11761 tatttgctaa tggagattct ccagtaccgc ttggcttgat gttttttgtc ttaggcaacc
    11821 gtaatggtag ctcttcttct gggacagctg tgactgttcc gtcctcccaa tggatgattg
    11881 ggattggttc accccagtaa cgttgacggg agaatagcca gtcacgtaag cgatagctga
    11941 cttctttttt ccctacgcca ttttcttcta gccattcatt cattttatcg attgcttctt
    12001 gtttgttcat gccgtctaga aattcagagt tgatgtgcgt accatcttct gtgtaagcag
    12061 ctttttcaac atctccacct tcgataactg gaagaatttc tagtccgaaa gtttgagcga
    12121 attcaaagtc acgttcatca tgtgctggaa cagccatgat ggctcctgtg ccgtatgaag
    12181 ataatacata atcggcgatc cagattggga tttccttgcc attaactgga ttgattgcat
    12241 aagcaccagt aaatactcct gttttttctt ttgcaagatc cgttctcttt agatctgatt
    12301 ttttagctgt ctcttcgata taagcttcta cagcagcttt ttgttcaggg gtcgtaattt
    12361 ctctaacaag atctagttca ggtgcaagaa ccgagtaagt tgcaccgaat agagtatctg
    12421 gtcgtgtcgt aaatacggta taggatttat ccgtaccagc cactttaaat tcaatatttg
    12481 ctcctactga acgaccgatc caatttcttt gcatttcttt aatgctttct ggccaatcaa
    12541 ctaattctag atcatccaat aagcgatcag cataagcagt gattttcagc atccattggc
    12601 gcattggttt tctgactaca tcatagccac cacgttcact tttcccatcg atcacttctt
    12661 catttgaaat aactgttcct aattcaggta cccagtttac cgctacttct gcttcataag
    12721 ctagaccttt ttcataaagc ttcgtaaaga tccattgtgt ccatttataa tactccggat
    12781 cagttgtatt cacttcgcga ttccagtcat aactaaatcc taaagaattg atttggcgtc
    12841 ggaatgtttc gatatttttt tgggtaaact cagctggatc gtttcctgta tctagcgcat
    12901 attgttcagc aggcagaccg aatgcatccc accccattgg atgcagcacg ttgtatcctt
    12961 gacttctttt cacacgtgaa aggatatccg ttgccgtata accttcagga tgccctacat
    13021 gtagcccttg accggatgga tagggaaaca tatccagtgc gtaaaatttt ggcttttcag
    13081 gatcatcatg ggtgttgaaa gtattcgttt ttgcccagta tttttgccat tttttctcaa
    13141 tttctttatg attgtaactc atttgctgtc ctcctgttct attgaaaaca cttgtttcta
    13201 ttataaatat aacgaaaaaa ccggcagaat ctgtttattt ttatttcctt ttgctttgtt
    13261 ttctcaaact tcacaaaaga aataaaaaaa tcctataaaa agattgctct ttttatagga
    13321 cgtaatctac gcggtaccac ctaattttac cttagtgaaa aggcctcgaa cgttgtaacg
    13381 gccgtcaccg ccgcttgctt actcgggtat tcagcaaggg caactcaaag gcgagttcaa
    13441 aagcatccgt gcacattctc accagccatg cgctctctga aacttcagtc tttttactac
    13501 ttccttttca agtgttgtca tatttaagta gccttatagt aacaaatatt tttgtggaat
    13561 tcaatcgtta tttcttcgaa cagctatttt atttagctac aatcaacgtt tgacctggat
    13621 aaataaagtt gttcttaata ttgttccatt cgattagttg agccattgta attccatgac
    13681 tgtcagcaac actccaaaca gattctccag atttcacagt ataggttttc gaaccagtaa
    13741 ctgattgttg gtttgttgat tgagatgaac ctttcttcac aatcacttgc tgtcctggat
    13801 agatgaaatt atttttgata ttattccatt cgattaactg atccatcgtg atatgatgtt
    13861 tgttcgctac gctccaaact gactcgcccg ctttcacggt atacatcgta ttaccagtgg
    13921 atgatgtact tgtcgtagca gacgtattcg gtgtatttgg tttcgctgca gaagtagttg
    13981 aacctgtatt tgtatttgcc gaagttccac ctttaacgat caattgctgt cctggataaa
    14041 tgaagttgtt tttgatatta ttccattcga ttaactgatc catcgtgatg ccgtttttat
    14101 ttgccacgct ccaaacagat tcacctgctt tcacggtata agtgccttga gacgaactgc
    14161 tgccagtatt aactgaactg ctgttatttg tattggttga cgaacctgta ttacctgacg
    14221 tgctgtttgt tgatccactg tttgaaacag tcagttgttg tcctgggtag ataaagttat
    14281 tttggatatt gttccattgg attagctgag ccatcgtaat tccgtattta tttgagacgc
    14341 tccaaacaga gtctccggct ttaactgtat atttcgttcc tgaagctgta ttagaagaac
    14401 ttgagctgga cgaactagag ccacttgtat tcccagtatt tgagccagta tttgctccag
    14461 cattagaaga accgccattt gatacgatta gctgttgtcc tggataaacg aaattgttct
    14521 tgatattgtt ccattcaatc aattgagcca tcgtaattcc gtgatcattt gagatcttcc
    14581 aaacagaatc accagattga acagtgtatt tcgcaccagt tgtttgtcct ccgtttgatg
    14641 taccagcatt tcctgaagag ttggtattgt taccggaatt attcgtcgaa gagccatttt
    14701 cctggctacc tttgattgtc agtttttgtc ctggataaat gaaattgttt tggatgttgt
    14761 tccattggat caattgatcc attgtgatac cgaatttatt tgagattccc caaacagaat
    14821 caccagattg gacagtataa gtggtcgaag ttgctgaacc tgagtttgta ttaccagaat
    14881 tgcttgaacc gccagtacta cctgcacttc cagtattatt gccacctgta gtcgctccac
    14941 cgcctgcgtt tccgctagct ggtgtatcat attgggtcaa tccgtaagtt gcgatgatat
    15001 tatttaattt gcctgcgtaa ccaggatcag tcgcataacg ccctgtcaaa caagctgtcg
    15061 catctgtata ggatttcgtg ttgctcttcc aagtaccggc atagtaataa ccgttgccaa
    15121 atgaagtatt tctaagtaca taggcattgt cattgaaaga ttcagcaaaa gaaggatatt
    15181 gtcggaaagg ctccttttta tttacccatt gattatttaa aaattcaagc gtatccatgt
    15241 agacggtttg tccttgatag ctgcctttta tcccaaaaag attataataa ggtgcttgag
    15301 ataacgcact actgccccaa ccgctttcta caactgcttg tgcgatcatc actgatgcat
    15361 atagatcgtt ggcttgagcg attggttgag cataagtagc aatttctgtg ataaatgccg
    15421 ctgtattgat tcttgcgccg aactgaacag gtgtttcatc cgcttgaaca ggaaccggct
    15481 gtactaatgg ggcagcgaca gaacagccta ccatcgcagt tcccacgatt gctgcccctc
    15541 gttttgcttt tcgaaaacgt ttggtctgtt ctaaacgtct ccgttctttt cttgcaatgt
    15601 tttccatctt ctatcctcca acttcaattt atctattatt aataattata acttctctta
    15661 atatttatta aaagttattt aagagctttg ttgttttttg taatcatatt gtaatttacc
    15721 gtaatgaaca acttctgttc attttcaagt tcttcctacg tctacgttct tgtttaaaag
    15781 gatttcgaaa aacactgatt ttatttccct aacatttcat ttgcattacc aaaaaatgtc
    15841 tttccgatta cagttatttt ttttataaga atattggtta ttcaaagagg aatcatacat
    15901 tggttttatc tctttaccgt ttttttcgct ggaccaaaaa tgctataata agtcattgct
    15961 tcaaaggagg aattgtatga aatataaaac atcatttcag gtggctggta gcttctttct
    16021 tgtcctgttt ttcattttgg gctggatcgt aaagttccat ctgtcgtggc tttctggttt
    16081 cgatcagtgg tttacaggca ttgtcagagc ttcttatcct gatctgaaca gctaccaact
    16141 ctttatgact aaattcggta atcctcttac cgtcatcatt ttatttgctt gtgcggcttt
    16201 atggatgtgg tataagaaaa aaagggcaga aacattttgg ttcgtttctg gatttattgt
    16261 aattgctgga atcatcaacc cggtgatcaa actatttttc atgcgagagc gtccttcatt
    16321 ggcgcactta gtagttgaaa caagttacag tttccctagt ggacatgcag caggcagtat
    16381 gattctttac ggttctttac tatttctgct tccagctttt gttcaaaaaa gaaccttacg
    16441 cacattgctt caattgctga tgatgtttat tatagtaagt gtcgggataa gccgcattta
    16501 tctgggcgtt cattttccta ctgatatcat cggcggctac ttactcagtc tttcttggct
    16561 gtgcctgact tatccagtgt atagagaaaa aagaaaacaa atagataact cttacagaaa
    16621 gaaggacttt taatgacttt tccttattca gaaggaaata aaagatatca ttcttggaat
    16681 tacgctttac gaaatgagtt tggcgggaaa attttcaagg tgccgatcga tggaggattt
    16741 gactgtccga atcgagatgg gactgttgcc catggcggtt gtactttttg cagtgtatct
    16801 ggttcgggag acatgatcgt tgcacctgaa gaccctcttc caatccaatt ccagaaagaa
    16861 atcgacatga tgcatcaaaa atggcctcat gtccaacaat acatcgttta tttccaaaat
    16921 tttacaaaca cccatgcgcc agttgaaata atcaaagaac gatttgagca agtagtcaat
    16981 ttaccaggtg tcgtaggatt atcagtcggt acacgaccgg attgtctgcc agatgaagta
    17041 gttgattacc ttgctgaact gaacgagcgg atgtatttat gggtagaact tggattgcaa
    17101 acaacttttg aagaaacttc aaaactgatc aaccgagcac atgattatca aacttattta
    17161 gatggcgtgg ctaaattacg caaacataat atccgtgttt gtacgcattt gatcaacgga
    17221 ttaccaggcg aaagtttaga aatgatgaaa gaaaatgtca gacgcaccat tttagattca
    17281 gatatccaag gaatcaagct ccatttgatg catctgatgc gcaaaacgag aatgttgcgg
    17341 gactatcatg aaggtcgcct tcaattaatg agccgtcctg actatgtcaa tgtcatttgc
    17401 gaccagctgg aaatgatacc aaaagagatc atcatccatc gtttgacagg ggatgcgccg
    17461 tgggattcct tgattggacc aatgtggagc ttgaaaaaat gggaagtctt gaatgcaatt
    17521 gatgaagaac ttcttcgcag agatagcttc caaggaaaat atgatgtacg gaaaaaggtg
    17581 agcgtctgat gttgcaaact gctttgcatt tcagccatac attactgcaa gaaatcttgc
    17641 aaccagggga tcacgtcgta gatgcaacga tgggcaatgg ccacgatacg gtttttctag
    17701 cagaacacat cggaaaaact ggtcatgtct atagttttga tatccagcaa caagcaatcg
    17761 atgctactcg tgaaaggcta gaacagcgtc agttagaaga acgtgtctca ttgtttctac
    17821 aaggccacga aacgttagga gaagtgatag ctgaacaaca aaatctcaaa gcagggattt
    17881 tcaatctagg gtatctccca aaaagtgata aagcaatcat cactatgcca gagactacac
    17941 gcacagctat ggaagaaata ttgaaacgac ttgtgcctcg cggacgattg atccttgtcg
    18001 tctactatgg tcatgaaggt ggagaaaaag aactggatat ggttgattct ttttgttcca
    18061 aactgccaca ggaaacatat aatgtgctga attatcggtt tatcaaccaa aagaaccagc
    18121 cgcctatttt atattgtatt gagaaaaaaa gataaataaa aaacgcaaaa cataaatgat
    18181 atgttttgcg tttttttctc taatactcag gttagagcgt tgatttcata acctctttat
    18241 tcacggtgct caaaagctta tatctgcggt aataagtcca accaa
//
LOCUS       DACECR010000058        18156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.058, whole genome shotgun sequence.
ACCESSION   DACECR010000058 DACECR010000000
VERSION     DACECR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.058"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            15..638
                     /gene="lexA"
                     /locus_tag="IWO17_001794"
     CDS             15..638
                     /gene="lexA"
                     /locus_tag="IWO17_001794"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ0290664.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            818..1036
                     /locus_tag="IWO17_001795"
     CDS             818..1036
                     /locus_tag="IWO17_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290665.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(1325..1837)
                     /locus_tag="IWO17_001796"
     CDS             complement(1325..1837)
                     /locus_tag="IWO17_001796"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ0290666.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1851..4148)
                     /gene="recJ"
                     /locus_tag="IWO17_001797"
     CDS             complement(1851..4148)
                     /gene="recJ"
                     /locus_tag="IWO17_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ0290667.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(4290..4724)
                     /locus_tag="IWO17_001798"
     CDS             complement(4290..4724)
                     /locus_tag="IWO17_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ0290668.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4734..5522)
                     /locus_tag="IWO17_001799"
     CDS             complement(4734..5522)
                     /locus_tag="IWO17_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0290669.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(5519..6460)
                     /locus_tag="IWO17_001800"
     CDS             complement(5519..6460)
                     /locus_tag="IWO17_001800"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ0290670.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(6637..7224)
                     /locus_tag="IWO17_001801"
     CDS             complement(6637..7224)
                     /locus_tag="IWO17_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ0290671.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(7445..8758)
                     /gene="obgE"
                     /locus_tag="IWO17_001802"
     CDS             complement(7445..8758)
                     /gene="obgE"
                     /locus_tag="IWO17_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ0290672.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            9046..10056
                     /gene="gap"
                     /locus_tag="IWO17_001803"
     CDS             9046..10056
                     /gene="gap"
                     /locus_tag="IWO17_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0290673.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(10141..11076)
                     /locus_tag="IWO17_001804"
     CDS             complement(10141..11076)
                     /locus_tag="IWO17_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0290674.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            11262..12221
                     /locus_tag="IWO17_001805"
     CDS             11262..12221
                     /locus_tag="IWO17_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ0290675.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(12307..13401)
                     /locus_tag="IWO17_001806"
     CDS             complement(12307..13401)
                     /locus_tag="IWO17_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0290676.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            13590..14351
                     /locus_tag="IWO17_001807"
     CDS             13590..14351
                     /locus_tag="IWO17_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0290677.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(14466..14906)
                     /gene="fabZ"
                     /locus_tag="IWO17_001808"
     CDS             complement(14466..14906)
                     /gene="fabZ"
                     /locus_tag="IWO17_001808"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0290678.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            15126..15881
                     /gene="fabI"
                     /locus_tag="IWO17_001809"
     CDS             15126..15881
                     /gene="fabI"
                     /locus_tag="IWO17_001809"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ0290679.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(15991..17004)
                     /locus_tag="IWO17_001810"
     CDS             complement(15991..17004)
                     /locus_tag="IWO17_001810"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ0290680.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            17169..18089
                     /locus_tag="IWO17_001811"
     CDS             17169..18089
                     /locus_tag="IWO17_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ0290681.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         5305 a         3879 c         3208 g         5764 t
ORIGIN      
        1 agaaggtgta ccttgtggcc cgacaaactg aatcaagaca aatcgaagtc ttacgatata
       61 tccatgaaca agtaacccaa aaaggatacc cgccgactgt ccgtgaaatc ggggaagctg
      121 ttcagctttc ttctacttct actgttcacg gtcatcttgc acggcttgaa aaaaaagggt
      181 ttatccaaag agatccgacg aaacctcgtg caatcgagtt gacaaaacaa ggtttagaaa
      241 agattgggat tcgtccaaca actattccag tacttggcgt cgtaacagct ggagaaccca
      301 ttttagcagt agaggatgct tcagacttct ttccaatccc tcctaatctt tcttccgaag
      361 aaggcagtct ctttatgctg acaattcgtg gagatagtat gatcaatgct gggatattgg
      421 atggtgacaa cgtgatcgtt cggaagcaag aaactgctca aaatggcgat attgtcattg
      481 caatgactgc cgaggacgaa gcgacttgca aacgattcta taaagaaaaa aactttttcc
      541 gtctgcagcc tgaaaacgac ttgctcgaac caatcatctt agatcaagta tctatattag
      601 gacgagtagt aggactttat cgtagtcata tctattaatt gctactaatt aaaagaaaac
      661 tttttaaaaa gagtttgaga cgttggtctc aaactctttt ttcgcccggg ttcttcttct
      721 ttcaaatttt tatcacttcc ctctttacaa accgaacaaa tattcgtata ctaaatatac
      781 gaacaattgt tcgcataaat taaagaggtg agaattcatg aaatcaatga gacgtcttgg
      841 cttagtatta actgttttgt ttttaggtat tattattggt aatttcggca tacgtccagc
      901 tttacttgtg tttgttccgt tatttacttt gtggttcatg ttgtgggatg aaaagaagta
      961 ccgtcaatct gagcggcgca gaattcatga agatcatttc tatcatgagc cctactacag
     1021 atccaggcaa tcataataag gattgactgc taattattca aaagacaaga agttaaactt
     1081 ttacgggggt gtgacaaaaa tcggtcacat cctttttttc gaataaacgg tgttctaaag
     1141 ccagtttttc ggtattaagt caaattgcgg aaaaagtgat tttttaaact tctactggtc
     1201 tttgcttgtt tccatttata aaaaaatagc gaccctcaaa agtcagatag agaatctgac
     1261 ttttgagggt cgctttaaaa tcgcttcttt aagaataaca tcaagaatag cttctttttt
     1321 ctgcttaata ttccattaat gtaatgatgt catatccttt gattttgtcg cgtccatgga
     1381 gatccatcaa ttcgatcaaa aatgcgcagc ctacgactac accgcctaat tgttcgatca
     1441 gttcgatcgt cgctttgatt gttcctccag tagctagcaa atcgtcacaa atcagcacac
     1501 gttgacctgg tttaatggca tccttatgca atgtcaatgt atctgttcca tattctaatc
     1561 cataagtcac ttcgatggtt tctcgaggta acttcccttt tttgcggact ggggcaaaac
     1621 cgactcctaa ctcataagca acaggacatc ctacgatgaa tccacgggct tctggcccaa
     1681 ctaccatatc aattcgtttt tcttttgcat aatttacgat ttgtttagta gcttcccgat
     1741 aagcgtcacc atctgccatc agtggggaaa tatctcgaaa cacaattcct tttgacggat
     1801 agtcgggaat actggcaata taatctttta aatccactct ttgtttcctc ctaggttgtt
     1861 aaccatttct ttatggtagg aatatcgctc aaaagcaaaa attcctcaac tttgatttta
     1921 ttgagccgtc gctggtataa tctgctctca gtcaaaggat gactttctgg gctagcgact
     1981 ttttgtaaaa caccgttctc tattgtaaca aatttcaatt caaaaaacac ctgtatcata
     2041 aaaattaata atttttcagg gatacgcaaa tattgagcta ttgcttttat tttatgacgt
     2101 atatcaattc gttcttgaga atggatcaat ttaaacaatt ttgcgtactg ttctcttgtg
     2161 cctaccccat tcaaataggc ctcatcaggt gaaatcccca tcagataaac acgattgaat
     2221 gaataagacc gaaaaacttc tttcagatcc gctaaatcat ctggacaatc cataacaact
     2281 agagagtttg gttttctttt ttcaattatt gcctttacag aatctagtcc ttcgaaaaca
     2341 atcgcagctt cctggataat ctcaggcacg aatttcaacg tcgaagaatc aaatgctaaa
     2401 taaaggctac cttccgacgt tcggctctgt tcacggaaac gttttgcccg ccaatcaaat
     2461 acttgcagac cacccacaga aaaatcagat accatcagct gcggtttctt acgcccgttc
     2521 cattcattga tcgaaagctt tcctaccaca tctacctgat tattcagcag ttcttcttct
     2581 tgagtaccaa aaccaaaagc gaccgcatca agctgactcg atgcatcgga aaggacaaat
     2641 ttcagatgct gttggtttgc tccgattctt ttgatatttt ctgcttggac ttgtcgaaat
     2701 aaaaactgag gcaaaggatt atccgtacca aatggcgcca atagtttcag ctcatcaatt
     2761 cgctctactg ttacttcatt tggcagcaat acttcatcaa ttctcaactc aggccctctc
     2821 atcaagtcaa tttgatgatc gactatatat tggttcatct tctcctgtag tatagtgaca
     2881 ttttcacttg gcatcgttaa accgactgct gcatgatgtc ctccgaagta agtaaataaa
     2941 tctcgcatct ggtcaagcat ttcaaaaaga ttcaaggctt caatactacg tccagatcct
     3001 ttagctgatc catcttcttt taaagtaagg acaagcgttg gtttacccgt ttcattcatt
     3061 attttcccag caacaatgcc caaaacgcct tcatgccatc ctcggtttac caatagatgg
     3121 atatgattct ccttattgac catcgctaat gcttcttgtg ttatctgctc tacgattgct
     3181 tttctttctt cattgatctc attcaatttt tttgcttgaa cggatgcttc ctcttcatca
     3241 aacgtcgcaa gtaacgagac ggcaggacgt ggatcgccca tccgaccgat tgcatttagt
     3301 ctgggagcaa tcgaaaaacc gatcgttgtt tcgtctgcgt ccctcatttt tacgccgctt
     3361 tcttcaaata gcgcctgaag tcctatccgt tctgaatgat gaatggcatc gattcctaat
     3421 gaaacaagga ttcgattctc atcagtcaat gaaactaaat cggcgatcgt tcctatcgct
     3481 actaaatcca aaaattctgc aggcggttct tccaataatg cagaagctac tttgaatgct
     3541 actccgactc ctgccaaatc tggaaatgga tagtgccccg ctggatgacg agggtgaaca
     3601 atcgcataag cgtctggtaa attctcaggt agttcgtgat gatctgtaat gatcacgtct
     3661 acaccagctt gctgagcgta tgctaccgca tcatttcctg ctaccccgtt atcaacagtg
     3721 atgatcaact ggattccttc ttcgatcttt tcttggtata ccgattggtt tggtccatac
     3781 ccatgctcaa atcgatttgg caggaatact tctacttctg caccaagtaa ttccagtgtt
     3841 tctttcatta ccgttgcact ggtgatccca tctgcgtcat aatcaccgta aacgaggatt
     3901 ttttctccac tgattacagc ttcttggatt cggctgattg ctttatccat atcgtgtatc
     3961 agatacggat catgtaattc ttgttccttt ggatgtaaaa atcggttgat atccttttcc
     4021 tcatggtaac ctctttgcca cagtaactta ccgacaaatg gagataattt cagttcacgg
     4081 atcatttgga gaaaggattc agaaggctct gtctcgctta acttccaatc aaaatttgct
     4141 tgtctcactt tctcactcct aacttagtta ttatagcaaa ggaaatcagc aaaaaaaagc
     4201 agaaagaaag aataaaaaca gatcagatcc gctagacaga tgttcagcag gacctgatct
     4261 tttgtacgac catttatgaa atatccctat tagtcaaaat aattgaagct ttgtttgggc
     4321 gattgagttt tttgttcttc cggcgctttc aattgactga tttgtgcggt tttcgcttgt
     4381 aactccgcct gataagcagc ttccaactcc gcacgttcgt ttctaaactc gcgttgctgt
     4441 tcttcttttg cttcttcaat tttcttttga agttctgttt gatattctga catttcctga
     4501 gtcagctgtt tgatttgctt tttttgctgc catagtgttg ccgaagcagt caaaaataca
     4561 atcagtgcac caatcagcgc agaaccgatg atcactaaga tcaatggcgc agaaaaggaa
     4621 gtgaagccaa aacttacagg aacattttta ttgttcaata cagcaaaaag aacaataatc
     4681 aaaactaaaa caaatccaag aatcacattg ctttgctttt tcaattcctt cacctacttt
     4741 ttgttaaaaa ttccgctggc taaataatca ccaatcgttg gaaacagcgt ataaaatttt
     4801 gatgcaactt ctaaaataaa cggttgattg atttcccgtt ttggatgtct catattgcgg
     4861 acgatcattt ttgccaactt ggcaggttct aaaacgattt gtccgacttt ttccaaatag
     4921 cttccgcttg gatcagcctt atcgaagaaa tttgtctcga ttggacctgg attcactgtc
     4981 gtaacagcta cgccaagtgg tttcaattct aatcgcaaag cgttggagaa gccaaggact
     5041 gcaaatttcg tggcagaata aaccgttgat tttgcagttg ccatttttcc agccatagag
     5101 gcgatattaa tgatatgacc ttgtcttctt tctgccatat cgatcgcaaa tttttgtgtc
     5161 aaaaccatca ttcctaagat gttgacttca aacatatcgt atgcttttcc aaggtcgaat
     5221 gtaaggaagt cttcgaaaat cccaaaaccc gcattgttta ccagaatatc gacgcgcccg
     5281 acttcttcca tcaccttttc atataattcg tccacgcttt caggattgct gacgtctacc
     5341 ttgaaagcat aagcctcttt gccgctcaag cgtctgcact cgtctctgac accttctaca
     5401 aatgccattc ttcgtgcgca acttataacg attgctcctt gctttgctgc ttcataacag
     5461 atttgtgcgc ccagaccgcc tgaactacct gttactaaaa caactttatc ttctaaattc
     5521 atgcctcttc ctcctcgcga aatggaatct caatgctatc aaaatctttc ataatcgtcg
     5581 tgttttcaaa cacttcttgt gcttcttttt caagctctaa agctgctttt cctaaatatc
     5641 tcgcactgat atgagtaagt agcagccgtt ttgcctgcgc ttcttttgca acttcagcag
     5701 cttgatgagt cgtggaatgg aaatagttgt gcgccattct tgcttcatct ttattaaatg
     5761 tgctttcatg taccagaata tcgctatttt ccgcaagcac aacggaatta tgtgtttttc
     5821 tcgtatcgcc taaaatagtc acgattcgtc cttttttatc tggacctacg aaatcttttc
     5881 cattgatcgt ccgcccatct tctagttgga tcgtttcacc ttgcttcagt ttgccgtaca
     5941 gtggtccagc tggaatacct aattctttta gacgatccac ctgcaattct cctttatgat
     6001 catgttcaac cacacgataa ccaaaactgt cgatcccatg atttagtttt tttgtataaa
     6061 cagaaaattg ctgatcagta aaaatcgggg ctgtttcatt tagctcaatg aatttcagcg
     6121 gataacttaa acgcgtttgc gaaataccta aagagacttt tatgtattcc tctatccctt
     6181 ttggcccgta aatctctaat ggcgtatccc caccttgaaa tgaccggctg ctgatgagcc
     6241 cgggaagtcc aaaaatatga tcgccgtgta aatgtgtaat gaaaatttta ccgatttttc
     6301 tcggacgaat agtcgttcgc aaaatctgca tttgcgtgcc ttcgccacag tcgaacaacc
     6361 aaacttcatt gcgctcgtct aaaagtttca atgctatact tgtcacattt ctatgttttg
     6421 caggaacacc tgcacctgtt cctaaaaatt gtaattccat aatctacctg actttcttta
     6481 agttcctctt taattttagg gtaaaaagaa gggataatca accgtaagtc gagaatgggt
     6541 tcaaattaaa aataacgttc taaagcgata gcgttttctt tgaaaattac tataaaaaca
     6601 gcagcgaacc cgtttgagat cctctgctgt tttttattaa tttgaagatc cagaactttc
     6661 attgtcgttg gaagtttgcg gatgtttgtc cttccaagtc ttttgtgcag cttctttcgc
     6721 atgattttca gcttctttcg catcatgcac atctttttcg acatcttcat aatccaagcg
     6781 agtaatttct ccgcctaatt ccatcattac caattgattc agtgggcgat tgtcctcttc
     6841 atcagcaatc aagatcagtc ctgtagctcc ttcaccgatt ttatttccta caaagtcgaa
     6901 cacggtctgg gcatctttta tttctttggc atcttgtgtc gcaccgatca ttcctccggc
     6961 aaaccatcct aataaaatcc ctagcggacc acctaataca ccaaccaaca ttccgattaa
     7021 tccacccgta gaagatttgt tattaccggt aaagtcgata aaatcttcta ttttgaattg
     7081 atgtgtccca ttaggatcat ggctgacaac tgccatctgt tctcctttaa tcgaacgaac
     7141 agcatgtaac tttttgattt ctgaaaatgc ctgatatgcc tggctttcta cttgaaaatt
     7201 taaaatgatt actcgtttgc tcattaaaaa cgcccctttc ataccttctt gttatttatt
     7261 ttacctttaa ttgaaaaata agaaaagaaa agcactcttt ttttccaata aaatagggct
     7321 gtgacaaaag tcgtatcaca gctccttttt ccgaataaac ggcgttcttg aagctgatca
     7381 cttctgtcac aacctccctt aaatctgaat ctatattttt tggtatttgt ttttatttgc
     7441 ttgcctattc gacaaattca aattcaaact caccgatacg tacaaggtcg ccatctttcg
     7501 caccgcgtgc ccgaagagct tcatccacac ccataccacg taattgacga gcaaaacgca
     7561 tcactgtttc atcatgatcg aaattcgtca tttggaataa tttttcgatc ttctcaccag
     7621 ataagatcca cgttgcatct gaatcacgat cgatttggaa ctcaggacct tctggattga
     7681 atccatagtg tacagtttct tcttccagtt cttcttcgta caatgggaat tctggcgtta
     7741 cttctaacaa atctgctgta gcattcaata acgcgtccaa tccttgtcga gtcactccag
     7801 aaattggaaa tactgggatg tcatcagcaa attcatcttc tttttccttg ttcagctgtt
     7861 ctttgaattt cactagattt tccggtgcat ccggcatatc cattttgttt gccacgatga
     7921 tttgcggacg ctctagcaag cgtaagttat aagtagataa ttctttgttg atggcaagat
     7981 agtcttcata aggatctcgt ccttccatcc cactcatatc gatcacatgt aaaattactc
     8041 gtgttctttc gatatgacga aggaattgtg tacccagccc aacaccttgc gaagctcctt
     8101 caatcaagcc tggcaagtct gctgcggcaa aacttctgcc atcacttgta gtaaccattc
     8161 ctagattagg tacgagcgtc gtgaaatgat aagcgccgat ttttggacga gcagatgaaa
     8221 taacggatag taaagtcgat ttcccgacag atggaaaacc tacaagtcca acgtctgcca
     8281 atactttcaa ttctagttcg atttttcgct cttgtcctgg ttcaccgttt tcagcaattt
     8341 ccggtgcagg attccgagga gaggcaaaac ggatattccc tcgtccaccg cgtccacctt
     8401 ttgccacaac tagtgtttga ccattttcaa ttaaatctcc taaaagagca ccagtctccg
     8461 catctcggac agtggttcct tgtggtactt taacataggt atcttctgaa ccacgaccgt
     8521 gcatcccttt actcatccca ttttcacctg gctgcgcttt gaaatggcga ttgaaccgaa
     8581 aatccatcaa tgtacgtaac ccttcatcga cgatcaagat gacatcgccg ccacgaccac
     8641 cgtctccgcc agccggtccg ccgtcaggaa cgtatttttc gcgacgaaat gcaaccattc
     8701 cgtctccgcc cttgccagct ttcacatcga tcgttacttg atctaaaaac atggacataa
     8761 ttaagtcctc ctattattca tttatttatt tgactgcctg aatttccaca ataaacaaaa
     8821 gtcttactta ttttatcggt gaaatctgat tttgtctatc atttcagcaa aaagattgtt
     8881 taccttcttc tttttttatc atttggtaag gtttccaacc ttttttctgc taccaagtat
     8941 aattttttga caaaatattc tttgcgtagc agaataacag tgtattaatt gtattaattg
     9001 aggatgccgt ttcacagcac ccctaataaa ggaggaactt ttgatatgac agtaaaagta
     9061 ggtattaacg gatttgggcg tattggccgt ctggctttcc gccgcatcaa agaagtctca
     9121 gatgatattg aagtagtagc aatcaacgac ttaacaagtc caactatgtt agcacaatta
     9181 ttgcaatttg actctactca tggtacttat cctggcacag ttactgcaac agaagactca
     9241 atcgtagtgg atggcgaagc aactcgtgtt tatgcagaac ccgacgcaag caaaatccct
     9301 tgggcaaaag aaaacggtgt agatatcgta ctagaatgta ctggttttta tacatctgaa
     9361 gaaaaagcaa aagcgcattt agatgctggt gtcaaacgag tagttatttc tgccccagct
     9421 ggtgctatga aaaccatcgt ctataacgtc aatgatgata cattagatga aaacgacaaa
     9481 atcatctcag caggttcatg tacaacaaac tgtttagcac ctatggctta tttcttaaat
     9541 aatgaatttg ggatcgaagt tggtacaatg acaacagttc atgcctacac ttccacacaa
     9601 atgctcttag atggacctgt aaaaggcggt aatttgcgtg ctgctcgttc agctgctgac
     9661 aatactatcc cacattctac tggtgctgcg aaagcaatcg gcttagttat ccctgagcta
     9721 caaggaaaat tacaaggaca cgcacaacgt gttccagtcg ttgatggttc attaactgaa
     9781 ttggtttcag tattgaaaac aaaagtaact gctgatcaag taaacgaagc aatcaaaaaa
     9841 cacacagttg acaatccttc attcgggtat gatgaccgtc aaatcgtttc tagcgatgtc
     9901 attggaacaa ctcaaggttc gatctttgac ccaacgcaaa cagaagtaac aactgctggt
     9961 gatttccaac ttgttaaaac tgttgcatgg tatgacaatg agtatggatt tacttgccaa
    10021 atgattcgct tactagaaaa attcgctaac ttgtaatttg atagatcaat cgataaaaga
    10081 agcctttgac tggatccagt caaaggcttc tttcttttca tcccctactt ttaaactcgt
    10141 tcaatgaaca tattggctta aaaattgttg gactacttgc tgatattttt caggatctgt
    10201 cttgtatgct tccgcatgct cggctccttt tacaacatac ttttcttttg gtcccttcgt
    10261 tgcacgatag accttatcta acatttcaaa tggtacaaat gtatcttcgt ctccatgtat
    10321 aaagagcatt gggcgctggt ttttcttcaa ttgttccact gcatttgctt caccgaaaaa
    10381 gtagccagca cggacttttg tcactagact cgttacttgc ataagcggaa atgctggaag
    10441 actaaacata tcgtctagct gataagcaag ctcatcatga acggaagaat aaccgcaatc
    10501 ttcaatgatt gctttcacat tatctggtag tttttctccg ctagtcatca taactgttgc
    10561 tgctcccatg ctgacaccgt atagaacgat ttcttgcgat tttccattgt tttctagcac
    10621 tttgtttatc cactgtacat agtctttacg ctctggccat ccaaatccaa tgtaatcccc
    10681 ttcgcttttt ccatgtcctc ttgcatcagg aacaaggaca ttgtaaccca tatcatgaaa
    10741 catctttgcg taattggtca tcgtttcagc gtttcccata tagccatgag ccacaagagc
    10801 tgttttttca cttttcgttt cagcaggaag ataaatcgct gataatttca gaccgtcttt
    10861 tgaagtgatg gtccaatcgc ttcggttttc ttttgaacta aaccatttat gtgcatccgt
    10921 ttctttggat gtctgtgttg taccaggtgt atcttcgtct aaaaagtctt tctcagaagg
    10981 cacaacagca taattataaa aataattccc tgcaaacact aacgcaacaa tcagagctaa
    11041 agcgacgcct aaaaggattt tcatccaaag tttcataaat catccatctc atttctcttt
    11101 tttcatataa ccttgaatta gtataatcaa agaatctcta ctatgcaatg ttcattttta
    11161 taacccgcga attgtaaaat taagttagaa aaataaaaag gcatttatgg tacactcaaa
    11221 ttgtatttcc gacgaaagaa aacaaaggag tgtaaccata aatgacctac aaacatctta
    11281 ccatagacga actgacaatg atagaatcat attatcttca acataataag ccggttgaaa
    11341 tcgctaaccg aatgggacgt gctatacaaa ctatttataa tgtagtcaat aagttcaagc
    11401 aaggcaagac tgctcttgat tattggcacc agtataaaga aaataagaaa aaatgtggta
    11461 gaaaagtcat tcaattacct gctcatgaag tagattacat taaagagaaa gtcactcttg
    11521 gttggacgcc tgacgtcatt atcgggcgaa aagaaaggtc tgtttcatgc ggtatgagaa
    11581 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
    11641 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
    11701 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
    11761 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
    11821 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
    11881 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
    11941 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
    12001 ctggaacacc ttctcaacgc ccattaaacg agaattctaa cgggattctg cgtcgtaatg
    12061 gactgccgaa atcaatggat tttagaaaag tgaatcagac atttatttcc agtgtcagca
    12121 atcaacgtaa tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt
    12181 tgagctatgt acaagaagca ttttattcta gcttaatttg acaaatcata acctataaaa
    12241 aaacaaaata aaaaagcaga aataactggc tatttctagc ggattatttc tgcaataact
    12301 aattatctat tttttatttt caattaattt aaagcttgac catggtttca aaaagtcaag
    12361 gaggaataat tcgtcatcgg agattcttcc tacaacattg acacgtccgt cgtttttcat
    12421 atccttcaag gcgatttgtg tttctccttt gtattgaccg tacccttcat tgtcaatcag
    12481 tacatcccca tgatgcatat ctcgcgtatt atgaggcggg aaatctttat ctttgtagta
    12541 tacacgagtc atcgttgaac gaaggatata ttctgagcga tcccctcgat aactatgaag
    12601 gttatcaaac aagcagattc gttcgttttc tgtaatacct tcttccgtat ctactttcaa
    12661 tgttggataa tccgcgttaa atgcttctgc catttctttc aattcttctt cacttgcgta
    12721 cgcatttcca atagaaatat catcaatcaa tcctgtaagg attaaatgct tcacttgtgt
    12781 cgcaatttca agatctcggt ggtcttccag cgtgcacaag ccatcttgcg taggccaagg
    12841 gccaaactca gcagattgcg aattgacgaa tgccatcgta ttcaaattat attttctgaa
    12901 tttttctgaa caaaaaacaa agtgttcgta tcctaaacca gagtatcgat gaggataaaa
    12961 attatgagaa cctaataaat tatccgtgtt tggcgaataa ctcatgatgt tgtcaacgta
    13021 attcgtgccc gaactcatat tgatctcgat tttgatacca aaaggattgc gcgtcatttt
    13081 tgcttcttct gctcctgtga atccgatatc taagcggacc ccgtaggcac ccatcttatg
    13141 gaaaaaagat aagtcatcgt aagaaatctc caactgttca aataacgctg gattgatatc
    13201 aaccattact tccatgccta ggctattcgc gtaatcaact acttctttaa attctgaaag
    13261 aactttttct ttatcatcat tgatctgcag caaactggta aaaacccgtt tatacccata
    13321 cttatgagct aaatccaagt acgctttgtc cttttcaaat gttgaccgtt ctggataaat
    13381 cgagattcct aattttccca tattaccatt tcctcctgca tcttttcgtt tttgtatcca
    13441 cttacattat agcaaacaca aagtaaatgg tctacacctt ttctttttct tttaaaataa
    13501 atgataaatt ccaataattt aagtaggtat tttccctcta ttttgttatc attaaagata
    13561 acaaaatagt aaattggaaa ggaacagtta tgaagaaaaa taaagttatt ttaagtatta
    13621 gtttagcagc agcattgctg tttgctgctg gttgttctgc aaacaacacg acttcttcta
    13681 gttcgtctac agaaagttca aaaagtacat ctactgcgga aagcacgact gccagtagcg
    13741 tcgatttgaa cgcattggaa ttaccacaat tgacgaaaga tgtcgccgaa aatgaagatc
    13801 tggtcgaaat ggtgacgaat aaaggcacaa tcaaaatcaa attatttccc gaacacgcac
    13861 caaaagcggt tgaaaacttt atgacacatg caaaagacgg ttactacaac ggactgactt
    13921 tccatcgcgt catcaaagat ttcatgatcc aaggtggcga tcctaaaggc gatggcactg
    13981 gaggggaaag catttgggga gaaggatttg aaacagagat caacaaccac ctttataata
    14041 taagaggtgc gttatctatg gctcgctcac aagatcctaa cagtaacggc agccagttct
    14101 ttatcgttca aaatacagat gatatgcatg atggtctttt aaaagacgac tatcctcaag
    14161 caatcatcga cgcttataaa aacggcggtt atccttcact agatggccat tatactgtct
    14221 tcggccaagt gattgaagga atggatgtcg tggatgctat tgcaaatatc gaaacagata
    14281 gttcagacaa accaaaagaa gaagtaaaaa tcgataaaat caatatactg caagaagcaa
    14341 aacaaaaata aaaaaataac gtctgaatcc tgcatagatg acaacgtatg ctaggattca
    14401 ggcgtcttct ttttacataa aaagcaagta agcttccttt tgttttgcta tatcaggttt
    14461 tatagtcaag cattttggtt gacgatgaac gtgaactcgc actcacagac ttttttatca
    14521 tctacatatg ctgcagataa cgctgtaccg actgatccct tcattttggt gatttcaaaa
    14581 tgacatttca gcacatctcc tggaacaact ttttgacgga atttggcctt attgatccca
    14641 ccgatataag ctctttttcc tagaaactct tctgatttta ggataagtat cgaacctgct
    14701 tgtgccagtg tttctagaat caacgcaccc ggcatcactg gattgtcagg aaagtgacct
    14761 tggaaaaaat cttcattgat tgttacattt tttgtcgcaa cgatcttttt tcctggttct
    14821 aaagtatcta cataatcaat ataacaaata ggataacgat taggaatcaa ttccattact
    14881 tcagtagcag ataatacgta agacataatt aaactccttc tttcaggcaa ttgccatttt
    14941 cattactttg actatcaaag tatcatattc ctaaatattt agcaatcttt ttttaattta
    15001 agaaaaaata gtttgacaat caaaacattt tgactaataa ttaagaaatc aagaagttat
    15061 tttagctatt attctctatt tttttacgac tatcaaattt ttatcagaaa caaaggagag
    15121 tcattatgtc atttctagaa ggaaaaaaaa tcgtcattat gggcgtcgct aacaaaaaaa
    15181 gcattgcttg gggttgtgct gaagcaatgt tgaaacaagg ggcagaaatt atctatactt
    15241 accaaaatga acgaatgaaa aaatcattag tcaaattggt cggcgaagaa gcattcactg
    15301 tagaatgtga tgtttctagc gacgaaagca tcgaaaaagc aatgaatgcc atcggtgaat
    15361 atgctgggga gatccacggc cttgtccatg cagttgctta cgccaacaaa gaagaattgt
    15421 ccggaaatgt gtctgatatt tccagagacg gctatcaatt agctcaagat atcagcagct
    15481 actctttaat tgctgtatca aaatacgcac aaccgtttct tgcaccaaac agcgggatcg
    15541 ttagcatgac ttatctaggt tcagaacgcg caatcccgaa ttataacatg atgggtattg
    15601 cgaaagcttc tcttgaagct gctatccgtt atttggcagc tgagttctcg cctaaaggaa
    15661 ttcgggtcaa tggaatctca gctggggcaa tcaaaacatt agctgtcaca ggtgttaaag
    15721 actatcaaaa actgatccag ttatctgaat ctcgtacgcc tgatcagcaa ggtgtgacga
    15781 tcgaagaagt cggtaatgct tgtgctttct taatcagccc actttccagc ggaatcatcg
    15841 gtgatattat ctacgttgac aaaggtgtcc atctttcata aacttttgtt gcattcttta
    15901 aagtaaaaca tataaaaatc aggaaaaaac tttttaaagt ctcttcctga ttttttcttt
    15961 ttacgttttt ttcaactcat tactttgaaa ttattccacg acttcaatcc agccttctgg
    16021 tgcttctatg tctccaaatt gaatacctgt caattcatca tacaaccgtt ttgttactgg
    16081 tcctacttcc gtttcactat aaaatacatg aagattgtta ttgtgataaa tcccaccgat
    16141 tggagaaata acagccgctg taccacatgc accagcttct gcaaattgat ctagatcatc
    16201 aatgaaaata tctccttcaa ttgcttgcag tcctagtcgt tcttcagcta actgcaacaa
    16261 ggaatatttc gtgatacttg gtaagataga gggcgattgc ggagtgatga attttccgtc
    16321 ttttgtaatt ccaaagaaat ttgctgcacc tacttcttcg attttcgtat gtgtggctgg
    16381 atcaagataa acacaatctg agtaatccag ttcttttgct tctgttcctg gaagtaaact
    16441 cgctgcgtaa tttccgccga cttttgctgc accagtcccc ttaggagcag cacgatcgta
    16501 atcagataca ataaaattcg ttggttttag tccacctttg aaatacgctc ctactggcgt
    16561 acagaagacg acaaataaat attctggagc tggatggaca ccaatgtttt caccgacccc
    16621 aatcagtaaa gggcgcaagt ataatgtagc gcctgttcca tagggcggca caaaatcttc
    16681 atttgctttt accacttgct tgacagcatc aacgaacatc ttttcaggga ccattggcat
    16741 cagcaatcgt ctagcacttt gattcatccg tcgactattt tgttcaggtc gaaagagatt
    16801 gaccttgccg tcttttcgac gataagcttt tagaccttca aaacattgct gaccataatg
    16861 aagcgctgga gagccttcat gaatcgtcag gtaattatct tctgtcaatt ctccttcttc
    16921 ccattgtccg tctttccatt tggcaataaa acgccaagga gttttgatat aagaaaaacc
    16981 tagattgttc caatcgattt gcatatactt accaccttcg ttattttctc cacattttat
    17041 cattatccat attatttgtc attttatttt cagctttttt ccttcattat agttattttt
    17101 gctatttcct tttttgaaaa cttcttggta caatacagaa agaatcaaat tttagaaaag
    17161 aggattttat gttttcatta ttagcaactg ccggtacgac agactcttct ggcgagacat
    17221 tgacagatca agctgtcagc aaagtaaatg cgtttcagcg gttttgggac gggattgatt
    17281 gggacgccat tttggctgcc tttatccaaa aaagtttata tctgattttt ttgatcgtcc
    17341 ttttttggat tcttcaccgt gtgggaaaat atctgattga taagtctttt aatcagtatt
    17401 ctaaaaaagc cattctaaac gagagccgct taaagacttt acatagtctt ttaaatacta
    17461 tttttcatta tacccttggt ttctttttta tctatgcaat tttatctgct atcggtgtgc
    17521 cgatcggatc acttttggct ggtgccggaa ttgcaggtgt ggcaatcggt cttggtgccc
    17581 aagggttcat gagtgatgtt attaccggat tttttattat catggaacaa cagatggatg
    17641 ttggggacta catcaagcta gcaaacttaa cgattgaagg aaccgtcgta tctgttggta
    17701 tccgtaccct tcagctgaaa gcaacggatg gtactgtcca tttcatcccg aatcgaaaca
    17761 tcaccacgat cagcaatctc tctcgtgcaa atatgcaagt acttttagat attcggattg
    17821 ttcctgaaga aggatatgac aaaatctatg agatcattga tcgagtaaat cgtacattag
    17881 ctgaaaaata ccaagaagag ctccaaactg aaccttctat ctttggatta gtggatcttg
    17941 gaaacagcaa ttttgcgatt cgtacggtct gctacgcatt gaacggaaaa caatatgcct
    18001 taaaagaaga gttcttgagc tcctatgtca aagaattgac agcttctggt ttcacgattc
    18061 cgaacagtcc aattgctgtc ggaaaataat ttggacaaac gatgcaacga atctcactaa
    18121 aaagagcgtg ggacaaaagt aaagaacact tttgtc
//
LOCUS       DACECR010000059        17404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.059, whole genome shotgun sequence.
ACCESSION   DACECR010000059 DACECR010000000
VERSION     DACECR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.059"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..382
                     /locus_tag="IWO17_001812"
     CDS             <1..382
                     /locus_tag="IWO17_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAQ0290682.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
     gene            complement(776..1783)
                     /locus_tag="IWO17_001813"
     CDS             complement(776..1783)
                     /locus_tag="IWO17_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0290683.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            2251..3657
                     /gene="lacG"
                     /locus_tag="IWO17_001814"
     CDS             2251..3657
                     /gene="lacG"
                     /locus_tag="IWO17_001814"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0290684.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            3680..4003
                     /locus_tag="IWO17_001815"
     CDS             3680..4003
                     /locus_tag="IWO17_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0290685.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            4014..5696
                     /locus_tag="IWO17_001816"
     CDS             4014..5696
                     /locus_tag="IWO17_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0290686.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            5902..6866
                     /locus_tag="IWO17_001817"
                     /pseudo
     CDS             5902..6866
                     /locus_tag="IWO17_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6876..7112
                     /locus_tag="IWO17_001818"
                     /pseudo
     CDS             6876..7112
                     /locus_tag="IWO17_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            7327..8079
                     /locus_tag="IWO17_001819"
     CDS             7327..8079
                     /locus_tag="IWO17_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0290687.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(8672..10129)
                     /locus_tag="IWO17_001820"
     CDS             complement(8672..10129)
                     /locus_tag="IWO17_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAQ0290688.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(10151..10453)
                     /locus_tag="IWO17_001821"
     CDS             complement(10151..10453)
                     /locus_tag="IWO17_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAQ0290689.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            complement(10510..10989)
                     /locus_tag="IWO17_001822"
     CDS             complement(10510..10989)
                     /locus_tag="IWO17_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0290690.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            11154..11927
                     /locus_tag="IWO17_001823"
     CDS             11154..11927
                     /locus_tag="IWO17_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0290691.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            11947..12375
                     /gene="lacA"
                     /locus_tag="IWO17_001824"
     CDS             11947..12375
                     /gene="lacA"
                     /locus_tag="IWO17_001824"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ0290692.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            12394..12909
                     /gene="lacB"
                     /locus_tag="IWO17_001825"
     CDS             12394..12909
                     /gene="lacB"
                     /locus_tag="IWO17_001825"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ0290693.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            12922..13848
                     /gene="lacC"
                     /locus_tag="IWO17_001826"
     CDS             12922..13848
                     /gene="lacC"
                     /locus_tag="IWO17_001826"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ0290694.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            13861..14847
                     /gene="lacD"
                     /locus_tag="IWO17_001827"
     CDS             13861..14847
                     /gene="lacD"
                     /locus_tag="IWO17_001827"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0290695.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            15303..15971
                     /locus_tag="IWO17_001828"
     CDS             15303..15971
                     /locus_tag="IWO17_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0290696.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            15962..17095
                     /locus_tag="IWO17_001829"
     CDS             15962..17095
                     /locus_tag="IWO17_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0290697.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
BASE COUNT         5651 a         2861 c         3674 g         5218 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccttcta acaattcctg gggccatgtc gcccacagag atattcaaaa tgtttgagat
      121 gggtgctgat attgttaagg tgttccccgc caactgtctt ggaccggaat atttcaatca
      181 agtgcaagcc cctttaggaa gtttaccttt gatggctgta ggaggggtag atcagactaa
      241 tgcacaaaac tatttgaaca atggagcctc ttatgtagga attggttcaa aattttttga
      301 aaaaagtgct gttcaccaac taaattacga gaggctaatg gagttggctg aatcttttat
      361 agatagttta agagttgaat agaacccatt tgcggaatac aacttgtttt ataatatggc
      421 gttatctgta taaaacatag tatcacagtg aatgagattg aactggtaga ttgatttttc
      481 gaccagttct ttttgcgcgc tctgatgctt gatatattcc caacgagata aaaaatcccc
      541 caagatacta agatgaagcg tttatccaac ctcatactta gataaaaagg aggaaatgtt
      601 ctgaatgata aacaaaaatt agcgcgcaca atttggcgat gaaaaaatca tattgtattt
      661 acgtcaaaat atcaccatca aattatttat ggaaaacata ataggcataa acccgattgc
      721 tcggctacta tgtggacaca attatagttt taaagataag tttcaattac ttattttaca
      781 cctcttcttt agggataaaa cttttcctaa cggtcaattt tgcgccgatt aatgttttct
      841 ttgatatgtc ccgcgaatat accagttgat cacttaataa atctacacag gttttagcta
      901 tctcttcgat atctatctta aaagttgtaa gtggtggtgc aacgaatttt gcgatgtcgt
      961 catcattaat acttattact gccatatctt tagggatgtt aatacccaat tcatggcagc
     1021 cctgaagaac gccggcagct atagtgtcag atggtatgaa acatgcctcg ggcatatctt
     1081 catttaactc agctttcatt ttaagagtta attgataccc ttcgtcaaca gtaaatgatc
     1141 cttgggagaa aatatacttt tcattgagta gtccgctctt ttctaaatga tatcggaagg
     1201 catattcacg gctatcaatc tcttctttac ccgtttctag gttaaagaaa tttcctccaa
     1261 taaatccaat ttttgtgaaa ccttcattaa taaagtagtt aattgcttgt ctggccattt
     1321 cgtggaaatc aggttctaca gtatcgaagt gttcaggtaa tgggtttatt cctcggatta
     1381 cccccttata ccctttttga tttaagtagt acaattcctt ttttgtcaat tgtcctattg
     1441 cgataaatcc agtaatatcg tcaggaatgc tttttacacc atcgttttta gagatgaaaa
     1501 tcaaatctag attagctttt ttactataat ttttcaaatc atctttgaca tgttgatagt
     1561 agacatcctt ttttaactgt tcctctgaga tccaaaaaag aatggcaatc ttgtacagag
     1621 tagtcttaat ttttgtcaga tcatatccta aagatttggc tgtaacgatt attttgtttt
     1681 tagtatcaga tgtaatagag agtgactcat cacctttaaa aagtcttgat atagtagccg
     1741 ttgagtaacc agattttttt gcaatatctc taatagtaac cattgttcat cctcctaggg
     1801 atataataat ccttttcttt gttaaagtaa atcgaaactg ctcttttagt aacttaaatt
     1861 actttttcta gcaggaaacg aaactctgat atcagcagag agcctaatcc attaacagag
     1921 aagattgaat cgataatgga tagccacttg gagaatgatt agtaggtggg attgatttta
     1981 gtgggaatca gtccgttttc ttgtttcgtg taaattaaat tagtaaaaag ataaatttat
     2041 tttacaaaaa aaacgtttac aaaacggttt ttgaatggta tatttatttt acatcaaggg
     2101 atataagtgc ttgtatatcg caataaaata gatattcgag gtcggttttt cgttgtgtaa
     2161 aaaaatttac ttatacatgt taaaattatg ctgaggtgat ttatggatta ttggcatatt
     2221 gatcaaacta attttcaagg agagattaaa atgaattata aaactttacc agaagacttt
     2281 attatgggag gagctacggc agcttatcaa gcagaaggct ctacaaaaga aggtggaaaa
     2341 gggcaagtag cgtgggatac tttccttgta gaaaagggac gctatactgc tgatccagct
     2401 agtgattttt ataatcaata tccagttgat gctgaattat gcaaaaaatt taaaattgat
     2461 agtattagga tgtctattgc atggagccgt atttttccaa aaggctatgg ggaagtgaac
     2521 caaacaggag tggaatatta tcataaggta tttgctgaat tgaataagca tggtattatt
     2581 ccttttgtaa ctcttcatca ttttgatacc cctaaagaac tatttgataa tggagatttt
     2641 ttaaatcgag aaaatgttga acatttcgta aactatgcac agttctgttt cgatgaattt
     2701 tctgaggttc attattggag tacatttaat gagatttatc cagttgcttc aaatcaatat
     2761 ttgatcggat cgtttcctcc agaagttcaa tttgatatag agaagactgt aacttgttta
     2821 cacaacatga tgtatgcgca cgcaaagaca gtaaacattt ttaaagatgg tggatataaa
     2881 ggggaaattg gtgttgttca ctcgctagag acaaaatatc cagcaagtga caaagaagaa
     2941 gataagcatg ctgcgaaatt agatgatgcg ttatcaatta agttcttatt agatgctact
     3001 tatttagggt attactcaga tgaaacaatg gcacatatta atgagatttt agcagcaaat
     3061 gggcaatctg cagatattga ttctaatgat tttagagaga tgaaaaaagc agcaaatcga
     3121 aatgattttc ttggtatcaa taactatggt agccattggg ttaaagagta caaaggagaa
     3181 agcacacttc actacaacac taccggagaa aaaggtagcc aagtctattg cattaaaggg
     3241 attggcgaga gaatcgtgcg tgaggatctt ccgaaaaccg attgggattg gatcatttat
     3301 ccaaaagggt tatatgattt acttgtacga atccaagacg aatatccaaa ttataataaa
     3361 atatacatta ctgaaaacgg gctagggtat aaggataatt ttgaagatgg gattattcta
     3421 gatgatgatc gaatcgatta tttacgccag cattttaatg cgatttccga tgctatcaaa
     3481 atgggagtta atgtaaaggg gtatttcatt tggtcattaa tggatgtttt ctcttgggtt
     3541 aatggataca ctaagcgtta tggactattc tatgtagatt tcgatacaca aaaaagatat
     3601 ccgaaagagt ctgcatattg gtataaattt gttagcgaaa caaaaacgat aatataaaaa
     3661 tagttaggag ggcaaggaaa tggataggaa agaattgcaa ttattaggat ttgaaattgt
     3721 tgcttatgca ggagatgcca gaagttcatt attaacagtg ttgaaggaag tgagagaagg
     3781 taattttgat aatgttgaac gattattagc ggatgctgat gaaaatttaa ccttagcgca
     3841 taactcacaa acgaaatgtt tagctgaaga agcgtcaggg aacgaaatgg aagtaggcgt
     3901 aatttttatt catgggcaag accatttaat gacaacgatt ctattaagag aacttatcaa
     3961 tgattttgtt gaattgtaca aagaaaaatt tgctaaagca taggagaaga aaaatgaata
     4021 cgattattaa gcaaattgag aagggaaaac cgttttttga aaaaatctca cgtaatattt
     4081 atttaggggc aattcgcgat ggctttttaa ctgctatgcc agctattcta ttttcaagta
     4141 tttttattat gattgcggct gttccagaag tatttaacgt gacgttacca gaggcattct
     4201 cgacgtggct ttggaaagtt tatggttatt caatgggatt agttggtctt ttggttgttg
     4261 ctacaacagc tcgatgttta gctaattcaa tgaaccgtca attatctgat tcaggtaagc
     4321 agattaatga agtttctgtt atgttagcgt caatttgtgg ttttgtaatg atggcagctg
     4381 atcaaattga tggtgggata gctactgatt atttagggac aaagggatta ttagcagctt
     4441 tcgtttctgc gtttgttact gtaaatgtgt ataaattctg tgttaaaaga gatttgacga
     4501 tccgtatgcc aaaagaagtt cccggaacaa tcacacagat gtttagagat attatcccct
     4561 tttcaattgc agtatttatt gccgttttga ttgatacggg agttcgtcaa ttttttggaa
     4621 catcatttgc agcaggtgta attcaattat tgcagccatt atttagtgct gctgatggat
     4681 atgtcggtat tgcaatcatt tggggagcca tggcaatgtt ctggtttgtt ggtgttcatg
     4741 gaccatcgat tgttgaacca gcgattgctg caattattta tgcgaacgta gaaactaact
     4801 ttgaattatt taaaaacggt gcacaagcct caaaagtatt aacagttggt ctaggaaact
     4861 ttgttggaac gatgggtggt actggagcga cactagttgt tccttttctg ttcatgttgt
     4921 tcgctaaatc aaaacaattg aaagcagttg gtaaagcttc atttattcca gtttcgtttg
     4981 cggtaaatga accgttacta tttgctactc ctattatttt gaatccgtac tttctaattc
     5041 catttttatt agcaccaatc gctaacgtat ggatattcaa actttttgtc gatgtcttgg
     5101 ggatgaatag ctttatatcc gtattgcctt gggcgacgcc cgcaccgatt ggcttggttt
     5161 taggaaccgg gtttagtgtg ttggcgattg tattagcggt agtattgatt gttgtcgatg
     5221 cgattattta tttacctttt gttaaggcct atgattctga attagtagaa caagagttaa
     5281 aaaacattgc tgcagaagaa gtaaaagaag aattggtcga agaaatccaa gaagttggtg
     5341 caactgcggc agttatagtt gatgaagcaa aagcatcggt tggtgaagga aagcgcgttt
     5401 tagtactttg tgctggtgcc ggaacgagtg caatgttggc taatgcatta gaagagggcg
     5461 caaaagagac aggtgttaat ctttctgcta aagcaggagc ttatggttct cattatgata
     5521 tgatgaaaaa ttttgattta atcgttctag cacctcaagt gaattcctat ttggatgatc
     5581 tccgtaaaga tgcagacaaa ttagggataa aagtaattgc aactaaaggt gcagaatata
     5641 tcaatttaac acgtgatcct aaaggagcag ttgattttgt attgaagcaa ttttagagct
     5701 tgcttagatt aataaagcgc tagccaactt agctagcgtt ttatcttaaa cacaatggta
     5761 agcgctatct ttttttgctg tttaaatttc gggtattctt tttgaggcgt cgattgtaaa
     5821 attaagctag acaactaaaa agatcatttg tgctacactc aaaatagttc cgaccaaaga
     5881 attaataagg agtgagtaca aatgacctac acccatctta caacagacga actagtaatc
     5941 atagagtctt atttcaatat cgagaaacct gtcgctcttg tcgcacaaag tttaaatcgc
     6001 tctagacaaa ccatttataa tgtttattcg tttctcaaac aaggaaaaaa gtgctagatt
     6061 attaccgtca atacaaacaa aataaatctc gttgtggacg acgttcaacc gttttatctg
     6121 atgaacaatc aacatatgtt caagaaaagg tagctcaagg gtggtcaccc gatgtgatta
     6181 tcggccgtgg cgagtttcca attgattgtt ctgttcgtac actctatcgg atgtttaaaa
     6241 aaggcctttt tgacacacag tcgttaccta tgaaggggaa acgtaaaccg aataatcatc
     6301 aagaaaagcg tggaaaacag gcctttcgca gaacgattca tgaacggaac agtctctatc
     6361 cacacctgac taaagaattt ggtcatctcg aaggcgacac cattgtggga gttaaacaca
     6421 agagcgctgt tattacattg gttgagtgtt tatccaaggt tattattact ttgaaaccga
     6481 caggcagacg agccgaagat atcgaaaaca ggttaaatga ttggttcaaa tccatgccta
     6541 aacacttgtt taaatccatg acatttgatt gtggaaaaga attttccaac tggcgatcta
     6601 taagtaactc gaacgatatt gccatctttt tcgcagatcc aggaacacct tcgcaaagag
     6661 ggctaaatga gcacgagcac tcaaacggct tgcttagacg agatggctta cctaaaacaa
     6721 tggactttag ggacatggat gaaaccttta ttcaatccgt tgcctcgaaa agaaaccata
     6781 ttccaagaaa atcattaaat tatcgaacac cattggaagt atttttgagc tgcatagata
     6841 aagacatatt gtctagctta atttgacaaa tcagaaaaga aaaaatgtat caagtttttg
     6901 aagaaatggg ttatccaatt aaaacggtca ccagaaaact agaagtaatc aaagcggatg
     6961 atacgatcag cgatttgtta gatgtggaag aaggagatcc aatgttctat ttccatacac
     7021 agggtttttc gctagaacgt ctgcccgttg agtattcgat ttcgaaatat cgaggagaca
     7081 ttaactactt tgtttttgaa cttgcacaat aacttcagat taaataaaat gatttattaa
     7141 agataaaagt cgaatgaaca aggctgtcac tatgacagtc atgctcgttc ggttttattt
     7201 tttagaatag cagcttgcaa attctgaaaa attttgcttt ccttcctgca gaaggcttct
     7261 taagaaaact tgttctagca cttttcttaa gaatttgata gtcttaaaaa gaaaagaggc
     7321 gatgaaatgt taaaaacgga tcgattccaa gcgatcatgt cgttattaga ggttaacggt
     7381 acagtacgag tcagcgatat tatggagaag ttttcggttt cggatatgac agtccgacgt
     7441 gatttggacg aaatggagaa acaaggatta ttagaacgcg tccatggcgg agcgaaattg
     7501 aaggattttt atcggcatgt ggagctttct catagagaaa aacagattat gctgcaagaa
     7561 gaaaagcagc ggattgtgga aaaggcgaat gaaatgatca atgatggtga tacgatattt
     7621 cttggaccag gaactacaat cgagctcatg tcaaaaatca ttcaagggga ttcactgcgg
     7681 attgttacaa actgtttgcc tgtttttgaa gaattaaata agcgaaaagg gaatcgaaaa
     7741 atctatctat taggtggcga gatgcgggaa acgactcaat gctttgttgg tgaaatcacg
     7801 aataaatcgc tacaggatat gaattttcat aaagcatttt tcagctgtaa tgctttaaat
     7861 gaacagaaaa ttatgacttc gacttttgaa gagggccaaa ctcaacagat cgctctgaat
     7921 aattctgttg aacgcttttt attgattgat cattcgaaaa ttgggaaaag agatttttca
     7981 gtatattata atctgaatga tgtaacagta gttataacgg atgatgatga acaaaagcac
     8041 tatgaaaaaa tcgaaaaaga agaaaaatat gttgtgtaaa tgagaaactg ccaaactcta
     8101 gataattaga acttggtagt tttttggtag ccttgaattt aagcgaatta tgttattttt
     8161 agggtgcatc attactaatt cctccgaata cttgaataca ccatttgaaa aaagatagtc
     8221 gatttttttg ggatgttatg tttatgtgaa ggaaaaaata gcagtatcaa tcaccgcttt
     8281 attaaggagg aaaagggata tcaaaacaac catggacact aagtcagctg aagctctttg
     8341 acatgaaacg agttagatca gaaaaagcaa tgatgaaatc gcacgcataa ccgcgggatg
     8401 cgaagcgatt caggaaaaaa gaaatcaagg aattcgcaat taaacaacaa aaagtcaaaa
     8461 agcacaaaac aaacaagaac acaaaaagaa tcgttgagga ataccaacaa aggcaagctt
     8521 aaaggagtta aaaaaagcat aaacgttgat aatttcttta ttgggtgtgg caagagatgg
     8581 gctatgcttt ttgaaatgga ttttacatct atttattttt tataggtaaa ataatacccc
     8641 gaagtctaaa gacattcggg gtgtttttag cttatttagc ttcggctgca taagtcatat
     8701 tatgcgccgt tgccggagat gcattctctt ctttttcagc tgcgtatgca gcgttgcgtt
     8761 ttttcatttg acgagcgtac caaatgaaga ttaaaaggta tgcacctaat gctaaggcag
     8821 cgtatatcac aaattcgatt tgtccttgag aagcattacc gactaaataa cctaggaatt
     8881 tttcaatcgg gccttccatc gttgtatgag agatcatggc ggacccgttc aagccggctg
     8941 gaaaggctcc gactgcttta gctgtttgag taacgaacgg ggcgatcaag gtgccggacc
     9001 ataagaataa tggtaattcg atcgcgccga ttacgatcat acggattaat tttccacgag
     9061 ttacgaccaa tagtgctggg gtaacaccca ttgcgatgat tccgcctagt ggcaataatt
     9121 tatttcctgg taagatgatg gcttctagca acatgattgg tgctaatacg ttagcggcag
     9181 cccagatttc agcgcggcct gctatgaaag gccagtctaa acctatgttg atggtacgtc
     9241 cttttagtcg tttattggca aaatcggtaa tcccttgtga taacggttct acggctgcga
     9301 tgaaccaagc gcctatcaaa gagaacaatt ccaaacagac ggcagcggta aaggctagcg
     9361 agaaaatttg cgttggtgtt tgtcctgcta gtaatccaac aaaaatcccc aaataaattc
     9421 caatcgcgaa ttttgatccc cagaagccaa ttttactatt caatttggca gcatcgaaat
     9481 catatttatc caaccaaggg aagattttgt cgaaaatttt atcgaaaacc ataataatcg
     9541 gattcatcat gtagttcata tgagtcgtgg tcatcgggtt gctgtctggc gcatttaaca
     9601 agtcattgaa ggttggtttc attaagtcgg agttccaaat ttttaataca ccaatgaaaa
     9661 taactaatgc agaagcaata aacagattag caccgcagaa gatcgcgaac aaaccaacaa
     9721 ttgctaaatg ccaaatatcg aaaatatcca catctaatgt gttggttttt ttcattgcta
     9781 acatgacaat attgacgatc accataatta gtaaaaaata aagcgtgtat ggcgaccccc
     9841 atgtgatggt tgctagtggg gcccaaccga catctgtaat gtttaatgaa atacctgtgc
     9901 gttcaacaaa agctgtcata gcgtctgaaa aagcagtagt taaaattcca ataatagctc
     9961 cgatcccagt cagggcgatc cctaatttga gcccgccttc aagagcgcgt gacggtttta
    10021 ctttaaaaaa taaagcaata acagttaata cgatggtcat taacggtgcc gcacctaaat
    10081 taattagggg ggtaaatact ttattggcaa tatcaataat tgtattcata tgcttttcct
    10141 ttctttagtc ctatttgttt aatgattgga ttgcgctttc catactttgg taaacaggct
    10201 cagccatagc aggaatacga taaagaatag cacctgcatc aacgacaggg atgttaatgt
    10261 caaaacccaa gtcggtgttg gcaatttgtg cgaaaatgtc ataatgagac agcatttctt
    10321 cattgatatc tttgatcata acggcgtcac aggtgacttg atagccacga tttttcaatt
    10381 cagtttccaa agcgcttttg atttgatggc ttgaattaac gcctgctcca catgctgcta
    10441 aaattttgat cataataatt ctccccttaa aataagttta ttttttatcc tgcggttagg
    10501 cagagacttc tatatataaa aatgttcttc taaaaaatga taaatcgctt ctgtgtcctc
    10561 taatttgaaa aacgaaaaca gtttttcttc gtcaacagaa ttaatgaaat ccatgatatt
    10621 agccaacatg ccagcctgtg cttcaccatt ttcgttcaag atcatgaaaa gaaatgaagc
    10681 ggaaatctga tcgcttggcg agatcatgtt gtagaaaggg atttcatttt ttactttgat
    10741 tggaatgata cgggtggttc gcacaaactc actttcggta tgagggatag cgatatctgg
    10801 caatatttta gagacaggtg agagatctaa tcccgtcgga taattacgct cccgttctaa
    10861 aaggttctcc aaaaaatctt cggttaccaa ttctttcttt aacagatctt gatagacttc
    10921 ctcgaacact tcttcctgcg actgcttgtc agaaacatag acagtgtctt tgaaaaagaa
    10981 ttcattcaca caaaacagct cctttattag ttcgtttatg tttatttata tgtttacaag
    11041 ttgagtataa ccgcttacaa aatcattgtc aacgcataaa cgaaaataaa cgaacaatat
    11101 gtgttgaaaa atgttcgtaa atgttttatt ataaggtcgt ggaggtacag aatatgctga
    11161 aaacagaacg acagcgttcg atcttagcga tgtgtgatcg ctatgggacg ataacagtca
    11221 aatttattca ggaagtactg aatgtttcag atatgacgat ccgccgagat ctagaggaac
    11281 tcgcgcagaa gaaaaagctt ttacgcgttc atggcggtgc acaaaaaatc gattatctgg
    11341 aaatgtcgac ttctatagaa gcaaatgagc tttctcatac tgaaaaaaag aagctgcata
    11401 caaaagagaa ggaatacatc gctaaaaatg cggcagctca tatcaatcaa gaagacgaca
    11461 cgatttttct tggatcgggt acaacgatcg aattaatgac acgatacatc aagccgtcat
    11521 ctctccgaat cgttaccaat agtctgccgg ttttcaatct attaagtgaa ataaaaagat
    11581 ttgaactgta tttgatcggt gggctatatc gggaaaagac tggagccttt gtcggttcaa
    11641 ttgccaatga agctgttcaa aaaatcggta ttaagaaggc ttttgtcgga gtgaatggat
    11701 tgaagggttc ggaggtttcg aatttcagta ttgaagaagg taccttgcaa aaaattgttt
    11761 tagataatgc ggcagaaaaa tgtttggttt cagatgcaaa caaattcgac catagcgatt
    11821 tttataattt ctatgatttg cgcaagatcg acaaattatt tacggattct actctttcag
    11881 ccgagctgaa gagttattat cagcaattca ctaaaatcat tcagtaaaaa ataggaggaa
    11941 acaaaaatgc gtgtagtaat tggttcagac agtgacggca tggaattaaa ggaattaatc
    12001 aagaaaaaat tgatcaaaga aggtattaca gtagtagata agagtgaaac ggcttcagaa
    12061 gattttgtgg aatcaacttt ggcgatcgca aatgatctga atgaaaacga aggtagttta
    12121 ggaatcgcgg ttgatggcta cggcgctggc agctttatca cggcaacaaa ggttaaagga
    12181 atggtagcag cagaagtttc ggatgagcgc agtgcgtata tgacccgtaa acataataat
    12241 tctcggatga tcacacttgg atcaaaaatt gttggcgatg agttggcgtt gaatattgtt
    12301 aaggaatttc tacaagcaaa ttatgatggt ggtcgtcatc aagtacgtgt cgacatgtta
    12361 aataaaatgg cgtaattagg aaggatgaat gaaatgaaaa tcgcaattgg ctgtgaccac
    12421 atcgtcacag atacaaaaat ggcagtatca gatttcttaa aatcaaaagg acatgaagtg
    12481 ttggatatgg ggacctatga ctttaccaga acccattatc cgatctacgg aaaaaaagtc
    12541 ggagaggccg tgactagcgg agaagcagat ctaggagttt gtttgtgtgg aacaggtgtt
    12601 gggattaaca acgctgtcaa taaagtacct ggtatccgca gtgcgctggt tcgcgatatg
    12661 acgactgcta tctatgcgaa ggaacaacta aatgccaatg tgatcggctt tggcgctcgg
    12721 attacgggag aatttttgat ctgtgacata ttagaggcct tcatcaatgc cgattatcaa
    12781 gaaactgaag aaaacaaaaa attgatcgcc aaaatcgatc acgtcgaaaa agccagtgac
    12841 aagcagtctg atccgcattt cttcgatgaa tttatcgaga aatgggatcg tggcgaatac
    12901 cacgattaag cgggtggaca aatgatttta acaataacga tgaatccatc gattgatatt
    12961 tcttatccat tagaaacctt caagctcgac acagtcaatc gagtaagaaa tgttcgcaaa
    13021 acagctggcg gtaagggcct aaatgtgaca cgtatcttga agcaattaga tacggaggtg
    13081 attgctagtg gtttaatcgg cggtttttta ggtgaagaga ttaaaaagga cctgactcaa
    13141 aatgagattg ctcatgactt tttacctatc agcggagaga cacgtaattg catcgctgtt
    13201 ttacatgacg gaaaacagac ggaaatccta gaaaacggac ctgttattac tgaacaagaa
    13261 gcaagggcgt tcttgcaaca tttcgaaaaa ttagctgtta aagccgaaat catttctttt
    13321 tcaggaagct tgccagaagg attgcctgct gatttttatc gacaaatgct gcaagtttgt
    13381 cagccaaagc ctgtcgtttt agattgttca ggtacagcat taagggaagt gttgcgaggc
    13441 agtgtaaagc ctaagctaat aaaacccaat accgaagagc tggctggtct gctaggacga
    13501 gaaatctcaa cagaattatc agatttaaaa gccgccttgt cagattcgat gtttgatgaa
    13561 attgaatgga tagtggtttc aatggggggc gacggagcct ttgccaaaca tcacgagact
    13621 ttttattgcg tgacaattcc aaagattgat gtcgtcaatc cagttggctc tggtgatgcg
    13681 accgttgccg gattgaccgc tgcgattcaa caaggtcttt cggatgaagc cgtgttaaaa
    13741 cagggtaatg ttttaggaat gctgaatgct caggaagcga cgactggcta tgtccaatta
    13801 gccaattatc aaaaactata tgatcaaatc ttagtggaga aagtgtagga ggatacggaa
    13861 atgaacgaag aaaaacgtca atatatcaag caactatgca atgaagatgg agtgattggg
    13921 gccttagcaa ttgatcaacg cggggctttg aagaagatga tcacaaaatt caagggcagc
    13981 gaagcagaag aatcagaaat cgttgaattt aaagaaattg tttcttcaca attaacgaaa
    14041 tatgcctcat cgattttatt agatcccgaa tatggcttgc cagcagctga cgttagagca
    14101 aaaaatgccg gcttgctgtt agcttatgaa aaaactggtt acgatgcgtc aactcccggc
    14161 cgtttaccag atttattatc gatctggtca gttaaacgtt taaaagaagc aggcgccgat
    14221 gcttgtaagt tcctacttta ctatgatgtc gatgaagcag aagaaatcaa taatcaaaaa
    14281 catgccttta ttgaacgaat cggctcagaa tgtcaggctg aaaacttacc cttcttcttg
    14341 gagcttgttt cttatgacgc gaacatctct gatgcgactt cgaaagaata cgccattaag
    14401 aaacctcata aagtcaacga tatgatgaag gaattttctg atccacgtta tggcgtagat
    14461 gtgttaaagg tggaagtacc cgtcaatatg aattatgtgg aaggctatgg gacagacgaa
    14521 gtcgtgtaca caaaggcaca agcggctaac ttcttcaagg aacaggctga agccactaac
    14581 ctgccattta ttttcctaag cgccggtgta aaagcagagt tgttccaaga aacactgcgc
    14641 ttcgcgaaaa atgccggctc aaccttcaat ggtgtacttt gcggccgtgc gacttgggca
    14701 gatggtgtag aaagctatat cgttgatgga aaagaagcag cgatggagtg gatgaatacg
    14761 caagggcgta aaaatattga agaattaaat gccgtattga aagaaactgc tacaccggtg
    14821 gaagtgttaa caagctcact atcataagaa atcagactgt gaagcaaaat tgcttgcagt
    14881 ctttttttat acataaagcg taaaatcatg ctgcattatt aggggaacga attctaaaat
    14941 agtaagtgag ttaaaacgaa ggggtttttc tttagagaga ttttcttaca tgtggtactt
    15001 aatgacttga tggcaatttt cagtattcta acgaggctta ttggtcataa accaacgaac
    15061 tttgaaaagt atatacaaga aaaccaagca aatagaaagt agtgagactg gaggaaaggt
    15121 tccagtcttt ttctattcta ggtattttca aacagtaaaa tcattcttaa ttatgtgttt
    15181 tttaacagaa tgtatgaatt tcttcaaatc ttcacaaaaa gaacgtggac tttgaagaag
    15241 agcgtgtact atgctaaaaa gcataagtgt aattactaaa tccgtatgag gagtgaatat
    15301 acatgcgtgt actggtggtt gaggacaatc gtgagctggt tttatccgta aaaaagggcc
    15361 tagagcagga gaatatttct gtagatgtag cttatgatgg cgagtcagga gaagagaagg
    15421 ccttcatcaa ttcatacgat gggatactac tagatttaaa tctgcctaaa aaagatggat
    15481 tatctgtttt acaaagtctc cgtgctgaag aagtcgaaag tccagttatc attattactg
    15541 cacgtgatga gattgaagaa cgagcaaaag gcttggattt tggagcagat gattatttag
    15601 tcaaaccctt tgagttacta gaattaaaag cacgaatacg cgcggttatt cgtcgttatc
    15661 atggacgtgc gctgccgcag atttcaattg gtgctttgaa gattgatcca ttaggtcgaa
    15721 gggtcagcta tcaaaagaca gaggtggtcc tgaaacccaa ggaatttgat atcttactgt
    15781 gcatcgctca gcgtcatcca gcggttgttt cgtctgagga aatttctgaa catgtctacg
    15841 atgaagattt tgatcctttt tcgtcggttc tgcgagtgca tctggctcgt ctaaaaaaga
    15901 aactggctga agcagctgaa aaagaggtct taaaaaccat tcgagcaaag gggtaccagc
    15961 tatgctatta aattttaaaa tgacgttaac gatgctggct ttttcactgt tggtgacagg
    16021 cgtgatttta gtgagtttct tctttctgag aaataatttg gagggaatca gtatcgcatc
    16081 taccttaggt gaagatgttt attttatgga agagggtaag acggttcaag cagagacgca
    16141 gatggaagct tcgaaatctt ccaacaatca atttatgact atttcgatga tagataaagc
    16201 ttaccaggac gaactattta aatatttacc agcgatggtg ctggttattt gttctgccgt
    16261 catgttatta acgtttattc tttggttggt tttacgcaaa atttacagta atcaaatctt
    16321 agcgattacg cgtgatttaa cgtttattga tcaagttcct aaaaatactg tgaaggacga
    16381 ggcattaaat gcggctttta ataagctaaa ggataagttc gatggtcatt tggaggatta
    16441 tcgacgatta aatagttatc tgacccacga gcagaaaaat gccatcgcta ttttgcgtac
    16501 gaatctggaa atagaacggc aacatgccga tcaaggaaat ttaatgcttt tagatcggat
    16561 gaccgacagt attgacgata ttttgacatt atccaataac tcagaggatc ttagcacgga
    16621 agtcgtagac gtttcactga tctgtgcgga agtttgtgat gcctacaaaa tgaattatcc
    16681 gaatcttttg tttgcgttta ctgaagatca accaaccctg attcatggac gagatcgttg
    16741 gatttatcgg gcggtttcga atttagttga caatgcaatc aaatatggtg aaggaaaact
    16801 ggtaattgtc accatttcaa accaaaaggg tagcgtgatt attgaagtaa aagatcaggg
    16861 aattggcatt cctcaggaga aacaggcatc aattttttct catcagtacc gagtgcatgg
    16921 gctcaagcag gatggcttac ctaaaacaat ggactttagg gacacggatg aaacctttat
    16981 tcaatccgtt gcctcgaaaa gaaaccatat tccaagaaaa tcattaaatt atcgaacacc
    17041 attggaagta tttttgagct gcatagataa agacatattg tctagcttaa tttgacaaat
    17101 cagatgtcta taaaattcgt tagaaaatac aatttataga tatccaattg atttgtaaac
    17161 aaaaaggagt tgtcaactaa gccactcctt tttgttgtaa tttttcccaa taagaaagtc
    17221 tactacttta ggaagataat agtaagatag aaatgttgcc cccaaaatta ttatccagct
    17281 ggatataatt aatcaaataa aaaggatcaa aaatgtagtg acccccaaaa gttagacttt
    17341 tttggagtgg agaaatccac tctttttttc atgctctaaa atgatatttt tacacaaccg
    17401 taac
//
LOCUS       DACECR010000060        17385 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.060, whole genome shotgun sequence.
ACCESSION   DACECR010000060 DACECR010000000
VERSION     DACECR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.060"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(176..1462)
                     /locus_tag="IWO17_001830"
     CDS             complement(176..1462)
                     /locus_tag="IWO17_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0290698.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="IWO17_001831"
     CDS             complement(1455..2723)
                     /locus_tag="IWO17_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0290699.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="IWO17_001832"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="IWO17_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ0290700.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="IWO17_001833"
     CDS             complement(3495..3941)
                     /locus_tag="IWO17_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0290701.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4034..4279)
                     /locus_tag="IWO17_001834"
     CDS             complement(4034..4279)
                     /locus_tag="IWO17_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290702.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4394..4972)
                     /locus_tag="IWO17_001835"
     CDS             complement(4394..4972)
                     /locus_tag="IWO17_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0290703.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5049..5579)
                     /locus_tag="IWO17_001836"
     CDS             complement(5049..5579)
                     /locus_tag="IWO17_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ0290704.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="IWO17_001837"
     CDS             complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="IWO17_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ0290705.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6436..6831)
                     /locus_tag="IWO17_001838"
     CDS             complement(6436..6831)
                     /locus_tag="IWO17_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0290706.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6828..8240)
                     /locus_tag="IWO17_001839"
     CDS             complement(6828..8240)
                     /locus_tag="IWO17_001839"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ0290707.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="IWO17_001840"
     CDS             complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="IWO17_001840"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ0290708.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(9095..10555)
                     /locus_tag="IWO17_001841"
     CDS             complement(9095..10555)
                     /locus_tag="IWO17_001841"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ0290709.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPKMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10725..11882)
                     /locus_tag="IWO17_001842"
     CDS             complement(10725..11882)
                     /locus_tag="IWO17_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ0290710.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11916..13286)
                     /gene="radA"
                     /locus_tag="IWO17_001843"
     CDS             complement(11916..13286)
                     /gene="radA"
                     /locus_tag="IWO17_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ0290711.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13349..13876)
                     /locus_tag="IWO17_001844"
     CDS             complement(13349..13876)
                     /locus_tag="IWO17_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ0290712.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14106..14912
                     /gene="proB"
                     /locus_tag="IWO17_001845"
     CDS             14106..14912
                     /gene="proB"
                     /locus_tag="IWO17_001845"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ0290713.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14909..16156
                     /locus_tag="IWO17_001846"
     CDS             14909..16156
                     /locus_tag="IWO17_001846"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ0290714.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16232..16576
                     /locus_tag="IWO17_001847"
     CDS             16232..16576
                     /locus_tag="IWO17_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290715.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16693..17019)
                     /locus_tag="IWO17_001848"
     CDS             complement(16693..17019)
                     /locus_tag="IWO17_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290716.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
BASE COUNT         4735 a         3726 c         3174 g         5750 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg aataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tctccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
     4021 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
     4081 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
     4141 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
     4201 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
     4261 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
     4321 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
     4381 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
     4441 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
     4501 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
     4561 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aattgctaaa ggttcttgca
     4621 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
     4681 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
     4741 tgcttataac gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
     4801 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
     4861 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
     4921 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
     4981 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
     5041 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
     5101 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
     5161 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
     5221 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
     5281 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
     5341 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
     5401 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
     5461 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
     5521 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
     5581 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
     5641 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
     5701 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
     5761 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
     5821 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
     5881 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
     5941 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
     6001 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
     6061 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
     6121 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
     6181 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
     6241 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
     6301 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
     6361 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
     6421 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
     6481 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
     6541 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
     6601 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
     6661 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
     6721 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
     6781 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
     6841 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
     6901 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
     6961 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
     7021 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
     7081 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
     7141 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
     7201 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
     7261 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
     7321 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
     7381 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
     7441 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
     7501 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
     7561 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
     7621 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
     7681 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
     7741 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
     7801 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
     7861 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
     7921 ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
     7981 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
     8041 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
     8101 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
     8161 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
     8221 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
     8281 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
     8341 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
     8401 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
     8461 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
     8521 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
     8581 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
     8641 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
     8701 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
     8761 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
     8821 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
     8881 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
     8941 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
     9001 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
     9061 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
     9121 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
     9181 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
     9241 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
     9301 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
     9361 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
     9421 cagcatagct cattttaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
     9481 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
     9541 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
     9601 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
     9661 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
     9721 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
     9781 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
     9841 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
     9901 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
     9961 caaaattata agttggcata ccatcgcgtt tttggatgac aaagtcgcca ccgatattat
    10021 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    10081 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    10141 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    10201 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    10261 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    10321 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
    10381 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    10441 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    10501 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    10561 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    10621 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    10681 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    10741 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    10801 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    10861 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
    10921 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    10981 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    11041 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    11101 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    11161 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    11221 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    11281 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    11341 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    11401 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    11461 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    11521 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    11581 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    11641 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    11701 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    11761 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    11821 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    11881 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    11941 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    12001 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    12061 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    12121 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    12181 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    12241 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    12301 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    12361 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    12421 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    12481 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    12541 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    12601 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    12661 atcccatttg ttttagcggt tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    12721 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    12781 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    12841 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    12901 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    12961 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    13021 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    13081 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    13141 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    13201 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    13261 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    13321 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    13381 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    13441 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    13501 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    13561 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    13621 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    13681 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    13741 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    13801 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
    13861 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
    13921 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    13981 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    14041 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    14101 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    14161 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    14221 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    14281 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    14341 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    14401 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    14461 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    14521 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    14581 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    14641 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    14701 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    14761 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    14821 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    14881 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    14941 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    15001 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    15061 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    15121 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    15181 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    15241 cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    15301 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    15361 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    15421 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    15481 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    15541 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    15601 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    15661 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    15721 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    15781 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    15841 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    15901 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    15961 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    16021 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    16081 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    16141 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    16201 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    16261 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    16321 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    16381 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    16441 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    16501 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    16561 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    16621 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    16681 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    16741 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    16801 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    16861 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    16921 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    16981 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    17041 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    17101 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    17161 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    17221 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac ggcgttcaat
    17281 cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat gagaagctat ttttccgttt
    17341 tgtttctcta atacttagga taaaacgctg attttacaac ctctg
//
LOCUS       DACECR010000061        17176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.061, whole genome shotgun sequence.
ACCESSION   DACECR010000061 DACECR010000000
VERSION     DACECR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.061"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(180..2087)
                     /locus_tag="IWO17_001849"
     CDS             complement(180..2087)
                     /locus_tag="IWO17_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ0290717.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            2217..3092
                     /locus_tag="IWO17_001850"
     CDS             2217..3092
                     /locus_tag="IWO17_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ0290718.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3172..4146
                     /locus_tag="IWO17_001851"
     CDS             3172..4146
                     /locus_tag="IWO17_001851"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0290719.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            4335..5471
                     /locus_tag="IWO17_001852"
     CDS             4335..5471
                     /locus_tag="IWO17_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0290720.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            5608..5952
                     /locus_tag="IWO17_001853"
     CDS             5608..5952
                     /locus_tag="IWO17_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ0290721.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            6223..7509
                     /gene="efmA"
                     /locus_tag="IWO17_001854"
     CDS             6223..7509
                     /gene="efmA"
                     /locus_tag="IWO17_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ0290722.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(7647..8246)
                     /locus_tag="IWO17_001855"
     CDS             complement(7647..8246)
                     /locus_tag="IWO17_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ0290723.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            8650..9771
                     /gene="mnmA"
                     /locus_tag="IWO17_001856"
     CDS             8650..9771
                     /gene="mnmA"
                     /locus_tag="IWO17_001856"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ0290724.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            10017..11438
                     /gene="arcD"
                     /locus_tag="IWO17_001857"
     CDS             10017..11438
                     /gene="arcD"
                     /locus_tag="IWO17_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ0290725.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            11452..12570
                     /locus_tag="IWO17_001858"
     CDS             11452..12570
                     /locus_tag="IWO17_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ0290726.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(12630..13922)
                     /locus_tag="IWO17_001859"
     CDS             complement(12630..13922)
                     /locus_tag="IWO17_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0290727.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            14072..14632
                     /locus_tag="IWO17_001860"
     CDS             14072..14632
                     /locus_tag="IWO17_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0290728.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            14761..15138
                     /locus_tag="IWO17_001861"
     CDS             14761..15138
                     /locus_tag="IWO17_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ0290729.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            15071..17143
                     /locus_tag="IWO17_001862"
     CDS             15071..17143
                     /locus_tag="IWO17_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0290730.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         5483 a         3113 c         3656 g         4924 t
ORIGIN      
        1 ccattttcac aaaatttttc ttactacttg aaactaagca gctttttcac aacctctaat
       61 acatggtatt ccagaagtaa cttcttcgaa aaataagccg aaatgtcaca aaaatttgag
      121 aagcgatttt cccaaattcc ttcttatttc ttgaagctga ccacttctgg cacaacctct
      181 taccagattc gcactctttt ttctggcgct agatacatag cgtcttgttc cgtgatacca
      241 aatgtttcat agaattcgtc aaggtttttt ggttgcacgt ttgctcggag tttagcaggg
      301 gcatggacat cgatttgcag taaaagttgt tggtattctt tttttgcttt tgtacgccaa
      361 atcgttgccc aattgacaaa gaattcctcg atagaaagat ctgattccgt ttttgctgct
      421 tccaatgcac agcttagacc gcctgcatca gcaatatttt ccgaaacggt taattttccg
      481 tttacttttc cgtcagcaaa aggcagacca tcgaattctt cgatcattgc ttgtgctttg
      541 ttttgaaatt gtgtaagatc ttcttctgtc caccagttgt tcaagttgcc atattcgtca
      601 aataaagcgc cgttattatc aaatgcatgg gaaatctcat gagcgataac agctccgatt
      661 ccaccataat tagcagagct tgattgttct agactgtaaa atggtgcttg aaggatcgct
      721 gccggaaaga cgatcacatt ccgaaatgga tgataataag cattgaccgt gtcagcactc
      781 atttcccatt ctgaacgatc cactggtttg ttccatttgc tgaaacgatc ttttaatgtg
      841 agtttgctga aaacaagagt atttgaaagc aaagtaccac cctgatctgc aggtactgtt
      901 ttgaactggt cgaataatgc tgggatctta tcggggtagc ctacttggat acctaagtta
      961 tttaatttta tgacggcttt ttcttttgtt gcatccccaa gccagtcatt ttcttctaga
     1021 cgttttttat aaacggcgat catttttttc accatttgtt ctacgtcttt tttagcagct
     1081 tcgccaaaat atttttttcc gtagtaatcg ccaactactt gatcgaattg tccagaggca
     1141 aggtaatacg ctgctttttt ctgaggcatt gcttcgtctg ttccagacaa tacgcgagaa
     1201 tagattccgc tgacttggcg gaattcttct gatagataac cactaagaga acgtactgtt
     1261 ttaatgatca tccagctttt gaataacgga aaatgagctt ctgtcaggat gtcattcaaa
     1321 tgatcgaagt acactggatc agttacgatc actttatctg gagttgtccc aatcaaaccc
     1381 gtgacaagtt ttttcaagtc cagtttatct gtgtatttaa taaatgtatc aaaagattgc
     1441 ggattataca ttttgctgta atctgcgttt tcttctgcac tttttacatg aggagcaatc
     1501 aatttatcaa attgcactgc ttgttccgtg attgcttctg cctgactttt ttctttgccg
     1561 gcaagctcta ataactgaac agccatatca aagaaaacgc ctattagttg tgcaccgttg
     1621 ggatggtctg atgcataata cgtcttatct ggaaggaata aagaaggcgg ataagcaaag
     1681 aaggcgttcg tttgcgcatt cttcatatct gcatctacat ccagattaaa tggcaatggt
     1741 aatgtatcca acagccattc tggaaattgc ttattcaatt cgtcgaaaga ctgcaatgat
     1801 tcaacttttt cgattatagg ttgaagcggt tgagcatgca actcatttct tcggtcataa
     1861 tcgtttgcca gttgataata tgcaaggaaa tgtttcattt tttctgaaga cactttttct
     1921 ggatgggcaa gcagatcgtc tgtttctccc atcaaaagtt ggtcgatccc gtcaactaaa
     1981 tcttggaaac cgcctgtagc tggtttatct gcagggattt ttgctgtttt ttgccattct
     2041 tcattgactg cttcatagaa atcttgtttc aatcgttctt tgttcattca tcgatcgctc
     2101 cttttttgtt tcgtgtgccc attatcttac aaaatgaagg aattaacaaa tagaacataa
     2161 aaaattgaaa attcgaacaa tcaaactgat ttgttttttt caatccttca cttataatga
     2221 aaaaaaggag gcgatcccaa ctgaatcaag tagtggataa acctttatat ttcaaagtag
     2281 ataaaaaaat taaaaaatta gccagcactc agcagctgca gtcaagaaaa agtaaattgc
     2341 tttttttagc tctggttttt gaagatcaaa gttatgtgat tatcgatcaa tcaggacatc
     2401 cggtagaata ttctccagca aaatatactt atcaagaggg tctttcttgt aaaaaatgga
     2461 agctgatcaa tgaagagcca atcgagttat ctagatggat caacagaaaa gaagatattc
     2521 ccgttttgat cgaagaaaaa aggtcaggaa aggaactcgc aaattgctgg gtgggattgc
     2581 cagaagaacg ttttttgagg tataagaaat gggctacccc ctcaggctat ctttgtggta
     2641 cttacgcggc agcagttctc cttgcctatt atcaagacta tcgtaagggg tggatgcttc
     2701 caaatgagat tcgcaagaaa aatacggctg attcgttggt tttgacgaag gcattgcgta
     2761 gccagatcca accactagga cttcctacga ttccttttca ggttagtaca gggatttcta
     2821 gttttttaaa gaagaatggc aatcatgagc gtgcacgagc aactcttcta ggttcatggc
     2881 agcgagctac aaaacgtata cgtgaaggca aacctgtgat gattggtatt ttgaaagtac
     2941 ttggcagtac atatggcaat cattgggtga cagcgtacgc ttattttgaa actgaaacag
     3001 gagaacgtta ttacaaagta cacgacaact ggggcgatta tcataaagtc attcctgcca
     3061 gttggagtaa cggaacagtt tcacttcctt aagaatttgc tgaaattaac agaaaatctt
     3121 ggaaaaatat gttaaactat cgaagaatag agaagttttg gggaggacta catgagtaat
     3181 aagtatcagg atgacacgtt gaagattttt agtttgaatg gcaaccgtcc attagctgaa
     3241 aaaatcgcga aagtgttcgg aacagaatta ggtaaaagcg tcgtgaagca atttagtgat
     3301 ggcgagattt ccatcaatat cgaagaaagt attcgagggg atcatgtgta tatcgttcaa
     3361 tcaacgaatg cacctgtcaa tgattactat atggaactac tgatcatgat cgatgcaatg
     3421 aagcgagcaa gtgcgaaaac gatcaatgtc gtattgcctt actatgggta tgctaggcaa
     3481 gaccgcacgg cgaaacctca tgaaccgatc actgcgaaat tgatcgctaa cttgatcgaa
     3541 gaagcagggg caactcgagt actgacttta gatctgcaca cggttcaagt acaaggattc
     3601 tttgatattc ctgtagataa tttatttacg atgccgttgt ttgctcatta ttaccgtcaa
     3661 ttaggtctga cgggagatga catcgtagtt gtttcgccaa aaaacagcgg tgttcagcgt
     3721 gcacgtagtc tttcagaata tttgaacagc acattagcca tcgtagatca tgcagatgat
     3781 gcttcaactg agggtggtta tgtgatcggt gatgttcaag gaaagacatg tatcatggta
     3841 gatgatatct tgaacacagg agcaacgctt gcacgagcag caaatgtatt aaaagaaaac
     3901 ggcgcaaaag aagtatatgc ctgtgcgtca catggattat tgtccgatca tgcgaaaagt
     3961 attttagaca atgcaccaat caaagatatt tgtatcactg attctgtttt cacagaagaa
     4021 aatcgtcatc cagaaaactt aacaatcgtt acatgttcag aattaatggg tgaagcggtc
     4081 aaacgcatcc atgaaaatac accgatgagt ccgttattcc gtctggaaga aaaagaattc
     4141 gaataaacaa aaaaaagagg gaaagaagtt gtgacagaag tgagcagctt caaggagctg
     4201 cgacaagact tttgtcacag ctcctttttt ctatgcccat gaaacgtcct ctagacaaaa
     4261 atgagctctt ctttggtttg ttcgctattt tatgctagaa tgtgcctagc aataatttgg
     4321 aagggatgaa tgaagtgaaa actatttatc tggatcatgc ggcaactacc cccatgcatc
     4381 cacaagtaat tgaagcgatg acggcaatca tgcaagacac gtttggaaat ccttcaagca
     4441 tacatggatt tggtcgccac gcgcatgaaa aattggaaac agcaagacaa atcattgcag
     4501 acagtctgca agcaaaacct cacgagatca tctttaacag tgggggaaca gaaggggata
     4561 atacagctat cttagaaaca gcgttctcgc gtcaaaaaga aggcagacat ctaatcacta
     4621 cagctatcga acatcctgct gttcttaata ccatgaaata tttagagaca aaggggtttg
     4681 acgttacata tctgcctgta gatgaaaaag ggaatctgtc gatcgaagag gtcaaaaaag
     4741 cgttacgaga agatacgatc ttggtctctg tgatgtacgg gaacaatgaa atcggaaatt
     4801 tgatgccaat caaagaaatc ggtgaacttt tagcggatca tccagcttat ttccatacag
     4861 atgcggtaca agcgtacgga aaccaaacga tccatccaga agaattagga atcgatttat
     4921 taagtatctc tgctcataaa atcaatgggc caaaaggcgt cggtttctta tataaacgag
     4981 acggagtaaa tatccctgca ttgcttttag gcggggagca ggaagaaaag cggcgtgcag
     5041 gtacagaaaa tttagcaggg atctggggta tggcaaaagc agttgaactg ctgtcacctg
     5101 aagaacgtga agcacgtaac aacaaatata aaggattcca gcaattgatt cttgatacat
     5161 tagaagcaaa cgaaatccct tatcaagtaa atggtgattt aacgaataaa ctccctcatg
     5221 tgttgaatct tcgcttaatt ggcgaagcaa atgatttgct actgatgaag cttgatctta
     5281 atggggtcgc tatttctact ggttcagctt gtacagcagg aaatatcgag ccttcacacg
     5341 tattagaagc aatgtatgga gaagatacac cgatactaaa agaatccatc cgtatcagct
     5401 ttggtttggg aaataccgaa gacgacatcc agtatttcct tgatcaatta gttcgtgctg
     5461 tcaaaaaata actagtgaag tcattcttat atgaacagat gtctgagaga acgccttcat
     5521 tttttaggga acttaactga aaaacctagt aaaaatcagg ataaactttt ataataaaga
     5581 gaaactaatt ttatcgaggt gaaaatcatg gcatttgaaa caacagcaac ggtactgggc
     5641 gcaccaatta gctatcgttt gcatcctgat gcaaaaagat atacattgcg cgataatgga
     5701 tttacagaaa caaacggagg caattatcaa ttgatccgac cattagatgc cacgccgcaa
     5761 agcaaagaag gattcaaatt aaaaatcact gtatcaaaag atattcaaac attgaaaatg
     5821 tcgatcacta cagcaaatgg attgaaagca gtcaatattt tcaaagatcc aaaacagaaa
     5881 atgagccaag ataaatttta ttttctgatg gatgggatga ttagccgtgg gttattcgaa
     5941 aaagtcgaat gatctgtgga tataaaaaga caactagttt tgaaacggcc tattgttaga
     6001 tgactaactt tgtaaggacg acgcaaatta gttgtctttt ttatttttaa atttaacaca
     6061 tgttgttatt tttaatattt tgctgattta ttgtttctaa gtaaagaaat ttaaattgaa
     6121 acgattgtta tttagggaaa aaggtatttt ttgaaaaaag aatcccgtat aatgattgtt
     6181 gtgcaaacgc tatacaaaat gaaaaagaaa agagggatgt ttatggaaaa tgaacagtcg
     6241 gtcgttttaa cgaattggaa gagaaattat ttgttctttt tatcgggaca gtttttgtca
     6301 ggaatcacta gtatggtcgt ccaatatgcg atcatctggt atttgacaag agaaaccggt
     6361 tcggcgacaa ttttgagttt tgctacttta ctaggaatga ttcccatggt tttactaagc
     6421 ccatttgtag gaccgctagt ggatcgctgg gataaaaaag ctcttttgat tgtcacggat
     6481 attattgtag cgattttcgc gcttatttta gcagtagtcg gaacgatttc agaatccttc
     6541 ccgatttggc tagtttttgt gtctttgttt atgcgttcgg tcgcacaaac attccaaatg
     6601 ccaacgatcc agtcgattat gccgacaatc gttccatctt ctcacataac aaggacgaat
     6661 ggacagttag gaatggtcca atcagcgaat ttcatcattg cgccagctct aggtgcggct
     6721 ttgttttcag tcgttcctgt caattacctt attttattag atgtattggg ggcagttttt
     6781 ggtgtcggct tattgatttt tgtgaagatc ccaaaagttt ctcctgaaat attggaagtt
     6841 ccgcttacta tttttaaaga tgcaaaattt gggctgcagc aattgatgga taataaaggg
     6901 ttatggtaca taacgatcaa tggtgcattt gtgatgctgt tgttcatgcc agcaatcagt
     6961 ctgtatccat tgatgacgct agattacttt ggtggttccg ttggacaggc gggagcagtg
     7021 gaagtagtct acgcagtcgg aatgctctta ggcggtgcac tgatcagttt tataggaaca
     7081 tggaaagacc gaatgaaacc tattattata gcctacatca ttatgggcct gacgatcggt
     7141 gcaagcggat tggttccaaa cgatagtcaa gggttcttgt actttcttat cttgaatgca
     7201 ggagcaggtt gtgcaacgcc atatttcaat acactgctga tggcaatgat ccagcagagt
     7261 tatgaatcga atgttttagg tcgtgtctta gggaacttca actctttgat gaacctggca
     7321 ggtccaattg gattattatt tgcaggacca ttagctgatc gtttaggggt agaaaaaatg
     7381 ttcttgtttt ccggaatcgg gattttatta tgcggaatcg ttttattttt aacgtcagct
     7441 gcccgaaatt atgataaaga attacagaaa aaactggtaa aagaacacca tgagcaaaaa
     7501 gacgaatagt atggaatgga gttgtgacag aagtcttgtt acaactccat ttctgatcct
     7561 aatgttcctg tggcaataag cctactcaaa tgaaaaatat gagtaattat ctttatttgt
     7621 ttaatacttg atatagggca gttttttcac gacctatcgt acttcctctt tgcttcatag
     7681 atttctttga tttgttgctc agataacgga gtgccaaatg gtagtttttc ctttaagagt
     7741 tctctttccg gtgcgtctac gcctacattg acatcgttct cgataggtac gctgtaatga
     7801 tggatatgtc catgaagatt gcggatagtg tcgttaggcc cgagcaacat cggataatga
     7861 gtcaagtaat attgttgatg gtcgaattta acgatggcac ctacatcata aaaataaaat
     7921 ttgggacttc cgtcagtaag cctaggatca tttttttcta aataacggaa aaaagcgcga
     7981 ctgtcatggt tccctttgat caagtggatt gttccttgca gttgagtgac cagttccaaa
     8041 atttctgcat ttgctttttc atattgcgga tgcatagcaa ggtcgcctag atgataaaca
     8101 tggtcggttt cttttattac tgcattccag ctagtgatca actgttggtt catttcatcc
     8161 acatttgcaa agagccgcgg agcaaagtcg ttttttccta ataagcgttc atggaaaaag
     8221 tgcatatcac tgataaagta attcatgtat cttttgcctc ccttttttct tagagtaaac
     8281 aaaatcactt atctgagcaa ctcataacat ctaaaaagaa aaggttgtaa atgagacgtg
     8341 cagctttaag cgataagaag caattttgaa aaatagtttt tcacaattta taaaattatt
     8401 ccttattgcc gaaaggctgg ccctttttat gagtatgcca attttttata tcgaatgctc
     8461 aaaagatgag tgattttttg gttgactatc atgtttgtgt actgaatagg tccagtagaa
     8521 cagtattttt acgacttgat aattaaggga tgaaattttc agaaatatga cgtataatgt
     8581 aaaatactga aaagttgcga ccttcaactc gtagatgttt cagaatctaa ttgaaatgat
     8641 ggtgatatca tgacagacaa cagcaaaaca cgcgttgtcg tagggatgag cggcggtgtc
     8701 gactcatctg taacagcact tttgttaaag gaacaaggat acgacgtgat cggaatattc
     8761 atgaaaaatt gggatgacac ggacgaaaac ggtgtatgca cggccacgga agattataag
     8821 gatgtagcga aagtagctgc tcaaatcggg attccttatt actctgtgaa ttttgaaaaa
     8881 gaatattggg atcgtgtatt tgaatatttc ctagccgaat accgcgcagg acgtacacct
     8941 aacccagacg tgatgtgcaa caaagaaatc aaattcaaag cattcttgga ttatgcaatg
     9001 gatcttggcg ccgattatgt agctactgga cattatgcgc aagtcactcg cgatgaaaac
     9061 ggcgtggtcc acatgcttcg tggagtagac aataataaag atcaaactta cttccttagc
     9121 caattatcac aagaacagct ttcaaaaacg atgttccctc taggcggcat ggaaaaatca
     9181 gaagtacggg caattgctga acgcgcaggc cttgcgactg ctaagaaaaa agattcaacg
     9241 ggaatctgct ttattggtga aaagaacttc aaacaatttt tgagcaacta tctgccagca
     9301 aaaaaaggaa atatggtcac tcttgatggt gaagtcaaag gacaacatgc tggcttgatg
     9361 tattatacga ttggtcaacg tcaaggacta gggatcggtg gcggaggaga ttcgcaagaa
     9421 ccctggtttg ttgtaggaaa agatctagcg acaaacacat tatatgtggg gcaagggttc
     9481 catcatcctg cattgtatgc cacaagcttg gatgcaagtg aaatccactt tacgacgaat
     9541 gaaccaatgc caaaagaatt caagtgtaca gcgaaattcc gttatcgtca gcaagacgtt
     9601 cctgtgacag ttcgcttatt ggatgataat cgtgcagaag tcgtattcga cgaacctgta
     9661 cgtgcaatca ctcctggaca agcagttgta ttttatgatg gaatggaatg tctgggtggt
     9721 ggtttgatcg accacgcata tcaagaaaca aaagttttac aatacgttta agcgatatta
     9781 aatgaatcaa aacagctatg gctaacaaaa gaaacacctt ttcttttgga tagcatagct
     9841 gtttttttgc aataaaatta atttttattc attttatact tgaaatactt gtgtaaaaag
     9901 ttcataatat ccaataatat gaataaagtt gctttttgtt aagcaagagc agttgcaaaa
     9961 tagaagggaa tcttttgcaa gtgatttgtt caattttata agtgagggtg atggaaatgg
    10021 aaaagaaatc gacgggtatt agcaaatggg gactggttgc tctggttgtc agttcgtcaa
    10081 tcggaagcgg tgtgtttggg atcacaagtg atctggctag ttctgcagct ccagggccag
    10141 cgatcctttc atggataatc gttgggattg gtattttagc tttggtatta tcgctgaatc
    10201 atttaggaga aaagcgtcca gatctagatg gtggtatttt cggttacgcg gaagcttcat
    10261 ttggtaaatt aggtggcttc atcagtggtt ggggttactg gttgtctgcg tggctgggaa
    10321 atgttgcttt tgcaacgatg ctgatgagcg ccatgggaga atttttcccg atattcaaag
    10381 gtggacaaaa tgttccttct attttatttg caagtatttt tatttggggt ctgactttat
    10441 tagtaaacaa tggagttgaa ggagcgagcg tcatcaatac aatcgtgacg atctgtaaat
    10501 tagtgccatt attcctattt ttaatatttg tatttatcgc atttaaagtg aatatattca
    10561 ctgctgattt ttggggtaac gtatctgata atctagtgaa tggaacaggg cagcaaggaa
    10621 cgatctggtt gcagattaaa ggttgtttga tggttatgat gtgggtcttt gtagggatcg
    10681 aaggcgcaag tgttttagcc aaccgcgcga agaaacgttc agaagcccag caagcatcga
    10741 tcattggtct gctttgttta ttgtttatct atatccttgc ttcgatcttg ccatacggcg
    10801 tcttatctca agaagagtta gcaactattt cacaacctgc tatggcaaat attttaaaag
    10861 gaatcgtggg aaattgggga gcagcgctga tcaatatcgg cttgattatt tcgatcattg
    10921 gttcttggtt gtcttggacg atgctcccag cagaaacaac gatgttgatg gctcgagacg
    10981 gtctgttgcc gaaaggttgg ggaaaaacga ataaaaaaaa tgccccaact tattcgttag
    11041 ttgttactgc aatcttgact aatctattct tattgacttt cttagttact gattatgcct
    11101 atcaatttgc ctattcttta tgtacagcgg caattttgat ttgttatcta cttgtcggga
    11161 tctatcaagt ccagttttct ttaaaagaag gaatcaaaaa acaagtagtg atcggtggga
    11221 ttgctgtagt ttttgaattg atgggcatca ttttagcagg attttcttat gtccttttat
    11281 gtagtattgc ctatcttcct ggtttttgct tctactggat cgcttgtcgt gaaaccaaac
    11341 atcaaattac gggaaaagaa aaagtaatga ttgggctgat ttgtgtggcc gcactgcttt
    11401 caatcgggtt gcttgctgta ggctggatca agatataagg gggaaagaaa catgaaaatc
    11461 gcaaatttcg gtgtagaaga atggctaaat aaatgggaaa agaaagcggt atatgatatt
    11521 gcacaaagct cgatcgaagc attgacatta gacgaactcg ttggcttaga tgggactagc
    11581 gtaagtaaat ttttcgaaaa acgcaaaaac gaaccattgg actatggctg gatcgaagga
    11641 tcagatgttt tcaaacaaga agtcgcttct ctttatcaaa ccgtcgatcc ggaaaacatc
    11701 ttacaaacga atggagcgac tggtgctaat cttttagctc tatacgcgtt agtggaagca
    11761 ggagaccatg ttgtttcgat gcttcctact tatcagcaat tatatgacat ccccaaatct
    11821 ttaggtgccg ctgttgactt tgttcatttg aaggaagaag aagattggca gtttgattta
    11881 gaacagctag aagccttagt caaacctgaa acaaaaatca tctgtttgaa tagtgcaaac
    11941 aatcctacag gcacattgct agatcggaaa actttagaaa aaatcgcaga aattgctcgt
    12001 acagtagatg catatgtatt gatagatgaa gtatatgcgc ctttaacgga taaaggtgaa
    12061 ttcttgtcga tcgtagatgt ctatgaaaaa ggtatagcaa ccaattccct atccaaaacc
    12121 tactccattc caggtattcg tattggctgg acagctactg gacctgagtt ggcagaagtt
    12181 tttcgaaagt atcgagacta cacgatgatt tgcggcggag tcctgtcaga tgatcttgct
    12241 gtacatgcat tgaagaataa agaaaaaata ttggcgcgaa acaaaaagat cattacagag
    12301 aaccttgtga ttttaaaaca atgggtagcc aatgaaccga aagttgaact tgtcgctccc
    12361 aattacgtct ctacttcttt cattaaatta gccatcgaag aagatgacca gacattttgt
    12421 atcaatttat tagaagaaac aggagtacta ttagtaccag gaagtgcctt tgatttaccg
    12481 aagcatgccc gactaggtta ctgttgtaaa aaagaaacct tggaaaaggg attattcctc
    12541 ttatctgagt tcttgaaaaa acaggcctaa acaaaaacac tgcaaggaca gtctgtgaca
    12601 actgttcaag cagtgttttt catttacgct tattgtctat gagaaggaga gtgccctgaa
    12661 gctatggaga ctcctgaagc tttttcttct gcagtttctt gctgatggat ctcttgtgta
    12721 ttgtgtcgta caagcaagaa gttgaccaaa acaagaagag aagtcgaaag aaagacgccg
    12781 cgataaccaa atgcactgga aacactggag ccgatcatag gccctactac gttaccagtt
    12841 gcttggaaag attggttata actgaagatc cgtccagcag cttgatgagg cgaatatttt
    12901 gtgatcaatg cttggacagc agggagcaga caagcatcag agataccaat caaaaaacgt
    12961 aaggcagcta attgccaaac attcgtgacg aatgccattg ggatataaac cagaatcgca
    13021 aaacccaaac caatcgcaag tattctttca ctcccaatct tgtcacctaa gcgaccaaat
    13081 cttggagcgg ctatcaaagt ggcaatccca ggtatcgaag caatgattcc gctgatcaac
    13141 gtaacattcc catgtccatg cagcaattgg cgaatgtaca gactgatgat tggactaatg
    13201 gaattgttgg atgcttggat gatcatcgtt gtgacgaaca taccgatgac gacgtgtgga
    13261 tatttcaagt ctttgaaaat ctgtttggca gaaaccatgt tttctttttt cactgggaca
    13321 aatttttcat ggacgaaaaa cagactcaac aagaagacga aaaataagat gatccctgta
    13381 atgaaaaagg tagggcgata gccaaacgct gaagcagaga tcccgccaag aagcggtcca
    13441 agtaatgttc ctgtaacaga accagttgct aaggttccaa gaacttgacc acttttttct
    13501 tttggcgtcc cagttgcgac aagggcagtg gcattactga tataaccaga aaaaatccct
    13561 tgaagcagac gcaatgcgac taattgatac acactagtca cgcaacccat caaagaaatg
    13621 acgattgcca ttcctaatga agcacgcaac agcatcaatt ttcttccttt ttggtcggct
    13681 aattttcccc accaaggaga gataagcgta gtcaccaaga aagtagatga aaaagtcaat
    13741 ccgctccaaa agttcaattg tgacgtacta taattaccta acgtatctat gtaaagtgac
    13801 ataaaaggca tgactaaact aaatccgata ccagccataa atgttccaaa ccataagaca
    13861 aacaagtttt gttcccaagt ttctcttttc ctaaataatt tttcctttat tcttgcagcc
    13921 aattagactc cttctttctg aaaattttca tctacagctc tttattataa actacaatat
    13981 gtagtttatc aatgaaagtc tcttttttct ctttcttttt cgtggtaaac tgaaagagtg
    14041 aaggatttat tttatgagag gaaacatgga aatgaaaaaa agaaatctgt ctcaagagaa
    14101 aatcatcgac tgctttcgag aactagcaga agagatggat gttcagcaaa taacctttca
    14161 gcatttagcc aaacgcttgg atatcaaggc tccctcgctg tataaccatt tcaaaaatat
    14221 tcgtgatgtc aaaacagcgc taacagcgaa acttctgcag gaacttaatg accagctgcg
    14281 acgggctctt gtgggaaaaa gtggtcagga agcactgcag atctatgcag aaacttatcg
    14341 atcctttgct ttttcaaatc aagcagtgta tgagttactg atcagtgtac cgcataccaa
    14401 tgaagctgtt ttattagatg gaatccatga aacgaatcag atcattttgc agatatttga
    14461 ggcttttcaa ttaacgaaag aagaaaaagt tcatcgaagc agggaacttc gtagtatgat
    14521 ccacggttat ctttccttac gttttttagg atactttaca aaagaaccaa caatcgatcc
    14581 agaagacagc tatttttgga tgatcaataa ctttatcgct actttgcctt aaacaatcag
    14641 attcatttgt aacaatttta agatgttcat taaatgaccc ttttataaga gaaaattctt
    14701 gcaactttct tgaaaagtga ctaagatgag tctagaaaag ctgaaagaag gagcacgagt
    14761 atgtatcaag taattaccat gttcggggac aatgagcctt ggtggttttt tgaagactgg
    14821 gaagaagata ttgaagaaga agaaacattc gagtctttcg aagaagcacg acaagcgtac
    14881 gaaaaacaat ggaacgaaat ccatcaaaat tatgaatata tcaatgcaaa aagtaacttt
    14941 ctcagcgcct tttggaatga cggagatgag cgctggtgtg aagagtgtga cgatgattta
    15001 cagcaataca agggacttgc gttgttgaaa aatcatcagc ccatcacagt agaaagcaga
    15061 aaggaatttt atgaaacaac taattctagc ggaaaaacca agcgttgcga aagacctaag
    15121 caaggtgctt ggtgctaatc aaaagcacaa aaattattat gaaggaccca aggtgatcgt
    15181 tacttgggcc cttggtcatt tattaggatt gaaaatgccg gaagatctga ataaggaatg
    15241 gcagagctgg caaatggaca cgttgccaat gatccctaaa aaactaggaa tcaaaccact
    15301 tcctaaaaca ggacatcaat taaaagcaat caagcagctg gctcagcgaa aagatgtctc
    15361 agaagcagtc attgcgacag atgccggaag agaaggggaa cttgttgctc gctggatctt
    15421 agagtatgtg catttcaata aaccagtgaa gcggttatgg atctcttctc aaacagataa
    15481 agcaatcaaa actggattca agcaactaag acctgcgaaa gaatatgatg cattgtatga
    15541 atctgctctt gcccgggcaa aagcggactg gctggtagga ttgaatgtga ccagagcctt
    15601 aactgtgaag tacgcggata atttatcagc aggccgtgtg cagacaccga cactagcatt
    15661 agtgagagac caagaaaaga aaatcgagca gttccgtcca caaacgtatt tcacgatcac
    15721 gttgcaagct ggaaaagaac aagccaaaat gcttcaaaaa aatcaatttg ctttaaaaac
    15781 acaagaagaa gcacaaaaac ttgttcaatt gatcagtaca agcaaaggaa ctgtcacaga
    15841 agtccaagaa aaattaaaaa cggaatctgc gccattgcct tatgatttaa cagagatcca
    15901 aagagaagcc aatcaacgct atggctattc tgcaaagaaa acattaagtc ttgtacaaag
    15961 tttgtatgag acccataaga tcgttactta tccgcgtaca gacagcaaat atctgacaaa
    16021 tgacatgaaa agtacgatga aagaacgatt gcaagcagtc agcgactttg ctcctgaggt
    16081 caagcagtat ttgaaaaacg gagccatcgt ccaacagaca aaagtattcc aagacagtaa
    16141 agtgaccgat caccatgcac ttttgccaac tgaaaacaga gcacgttatg aaaaattaag
    16201 taatgaagag cgaaaaatct atcaaatgat tgtctctcgt tttttaggac tgtttgctca
    16261 accccataaa gtcagccaaa caaaagttac ggttgaattt ggtaaagaac gattcatttt
    16321 tcggcaaaat cgtgtcgtcc aagcaggatg gaaaggcgaa actgaaactg agacggaaac
    16381 agtcaaatgg gaaaaaggaa tgagaatcaa tcccgatttc gccatcaaaa aagaactatc
    16441 tgcaccgcct aaaccattgt cagaagcaag cttgcttggt caaatggaaa aatacagtct
    16501 gggaacccca gctacgcgtg cagagattat cgagaaacta atcaaatctg aactgatgga
    16561 acgtacccct caaggactgc aagtatctcc taaaggtaag caattattag tattggtaaa
    16621 tccctcttta gtaacaccag atttgacaga aagctgggaa aaagaccttg aagctattgc
    16681 tgcaggaaag aaacaagcag gaaccttctt gaatggaatc gaaaaagaaa caaaacgctt
    16741 ggtgaatgag atcaaatcaa gcaaacaaga atatcaagat ttctcgatta cccaaaagaa
    16801 atgcccagaa tgtggggcca atctgcgcga gaaaaacaca cgtgacggca aaatctatgt
    16861 ctgcaccaat tcagattgca actaccgcag aagaaaagat ccaaaagtct ctaatcatcg
    16921 atgcccgcaa tgtcaccgga aaatggaaat catcgaaggt aaaaacggtg cttatttccg
    16981 ttgtaaatac gatggaacga cagaaaaatt gcttgataaa aaagagcaga agaagaaaat
    17041 gaccaaacat gaagaacgac gcttgctgaa aaaatatgca caagcagaag aaccagaaga
    17101 aagcccatta gcagcagcac taaaagcagc aatgaagaag taataagata tgaaagaggt
    17161 tgtgatagaa gtggtc
//
LOCUS       DACECR010000062        16854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.062, whole genome shotgun sequence.
ACCESSION   DACECR010000062 DACECR010000000
VERSION     DACECR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.062"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..454)
                     /locus_tag="IWO17_001863"
     CDS             complement(<1..454)
                     /locus_tag="IWO17_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0290731.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
     gene            complement(559..1422)
                     /locus_tag="IWO17_001864"
     CDS             complement(559..1422)
                     /locus_tag="IWO17_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0290732.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1618..3717)
                     /locus_tag="IWO17_001865"
     CDS             complement(1618..3717)
                     /locus_tag="IWO17_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0290733.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3937..5046)
                     /gene="rpoD"
                     /locus_tag="IWO17_001866"
     CDS             complement(3937..5046)
                     /gene="rpoD"
                     /locus_tag="IWO17_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ0290734.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5070..6911)
                     /locus_tag="IWO17_001867"
     CDS             complement(5070..6911)
                     /locus_tag="IWO17_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0290735.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7093..9486)
                     /locus_tag="IWO17_001868"
     CDS             complement(7093..9486)
                     /locus_tag="IWO17_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0290736.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9593..10453)
                     /locus_tag="IWO17_001869"
     CDS             complement(9593..10453)
                     /locus_tag="IWO17_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ0290737.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLCGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10512..11033)
                     /locus_tag="IWO17_001870"
     CDS             complement(10512..11033)
                     /locus_tag="IWO17_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ0290738.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11353..12072
                     /locus_tag="IWO17_001871"
     CDS             11353..12072
                     /locus_tag="IWO17_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0290739.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12098..14689
                     /gene="mprF"
                     /locus_tag="IWO17_001872"
     CDS             12098..14689
                     /gene="mprF"
                     /locus_tag="IWO17_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0290740.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14877..16064)
                     /gene="tuf"
                     /locus_tag="IWO17_001873"
     CDS             complement(14877..16064)
                     /gene="tuf"
                     /locus_tag="IWO17_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0290741.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16432..16788
                     /locus_tag="IWO17_001874"
     CDS             16432..16788
                     /locus_tag="IWO17_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0290742.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4631 a         3497 c         2956 g         5770 t
ORIGIN      
        1 tttctttttc atagcattcc tgacagattc catggaacgt tagccgatga tcttttacta
       61 aaaaatgata acgttcttct actacttgct ccacttcaag taaaagatcc tcttctactt
      121 cctcgatcgt gccgcagttc aagcatagta aatgatgatg gaaatgtttg gcgccttctt
      181 ttctcaaatc ataacgggct aatccatcat taaaacttac tttatcgaca attttcaatt
      241 ctgtcaagat ttcaagggtt cgataaacag tagcaagtcc aatttccgga ctcttttttt
      301 tcactaaaaa gaagatttct tctgcagata aatgctcttt ctcattttcc aaaagcacaa
      361 gcagagtagc ttctctttgc ggtgtcagct taaaccccgc ttcatgtagc tgctgttttg
      421 tttttttcat cgccgaaata gcattcatga ccatttactc aaccccaaca atttataata
      481 attacaaata aaaaaagata taacactatg ttaattaaaa ttattataag ctagttaaga
      541 aaaacatgca agggattctc aattagattt tttgactagt tttgtcacac cgttttcttg
      601 tgtaatcaat ccttgtttca aaagattacc taatgcgcgc ttaaatgctc ctttgctgat
      661 accaaaagct ttcatgattt cttctggatc cgatttatct gtaaagttga tttgctgatc
      721 tgcagaacgt tctaaaatgg ttaaaatcat ttgtgcatca tctgatatag cttcatatgc
      781 tcttggcttc aatgaaagat tcagtacacc gtctggtcgg acaccaatca ctcgaccttt
      841 tatctgctcg cctagacgag gttcattata tcgttcagaa ggatggataa acccaagata
      901 gaaatcttcc gttaaaacgt acgtacctgt taatttcaag cggtatgctg ttcctatcac
      961 atctttgttt ttcatttctt ctgttccata tttgcttaaa gatttgaata ttttttcatc
     1021 tgctaaactg ccccaaatac ggtcttttgc atcaactcta agggaaatca tcaagcgatc
     1081 ccctttttta ggccatagct ctgtcattgt cggcaattca tcaagagata cgacaaagtc
     1141 tttatctgga agaccgatat cgacaaacgc acctaaatct tttcgtgtag cagtaacgat
     1201 tccaaaagcg taatgaccga tacggctttt aggaatttgt gtagtgatcc gattttcatg
     1261 tttttgattt tggtaaccga atccttcaac tgcttcaccg attttgtgtt caccttctga
     1321 tttgcttaag ccaaaggttt gtccgttttt ttggacaaag tagttttttt cattttcatc
     1381 aataataata cctgtaaata tagttgctaa taaatcgttc atttttttct ccctctatag
     1441 tgaaaggttt tcttaataca tttctttgct atcataacat aatgttatct acttgaatag
     1501 ctgagccttg gtttggaaag gtgaaaaaag gagtatgaca acagttgtgt catactcccc
     1561 tttttcgaat aaacggtgtt aaacggcagg ctcacagcct ctttcatttg cctaaaatta
     1621 attgctccca tttcctccga accatccagt tttacagttg atggttacga tgtaaaatgg
     1681 caaagcactt tctccaatgc ctttcatcaa tgcagatttt gttgaagtgg catatagatt
     1741 ataatagcct tccccatttt cgattctggc tttttctaat ctgtaacctc cttcaacgat
     1801 atatccacgt tggatacatg tagcaatcac tttgctttgg acacctggtg cccacgtcca
     1861 tgctggatta gatgcatcgg caatatcact tgctctggca gatttgattg gctgattcgc
     1921 tgagtttgaa gaggaagggg tcgaggatgc cgcttcttgc tgtgctgctt gtgcgaccgc
     1981 agcttgttga gcggcttgag ctgcagctgc tgcttgagcg gcttgttttg cttgttcttc
     2041 agctgctttt ttcgcttctt ctgctttttt cttttcctct tcttcttttt tctctttatc
     2101 agtgagcatc ttatctactt gtttcaattg ttcattcaac tgagcagata gatctgtatt
     2161 tttcactttt tctactgctt cttttgccgt cttgtattgg tcgcgtgttg ctgaatccac
     2221 tacttttccg tctttaaaga ctatagctgt tttttcttta gctgtttgca attcagcata
     2281 ttgattttta gcttctgttt gtgcttgttg aatcaatttt ccaaaatctg tgttatccgt
     2341 ttcgattgct tggatctctt tatctgcttt caacttgatg tcatctgcta gatgatcatc
     2401 gacgatggca ggtgctatga accactcatt cactttttgg atatctcgga tcttagattg
     2461 gatatcttgg taagttgttt ctaactctga atatcctttt tcattcttaa gactggataa
     2521 atcatttttt agttttgata aatcttgatg aatcatatct gcacgaataa attgatggtg
     2581 atcatctgta taaaaggcag caagtttctc atgaagttct tccatttgtt gctgtctttt
     2641 tgcttctgct gctacttcgg cttctacttt ttgatgatgg tcataataag cccagcttcc
     2701 tccaccgagt agaagtactg ctgctaaaac gagataaggt actttttttg attttttctt
     2761 ttgcgcttta tctggatctg ttttttctaa gtttgattct tgatgtgttt tttcagtatc
     2821 tggcacgatt gttattgatt tcgcttctgc ttcaaacagc ttaggctggt tctcttcttt
     2881 ttctattttt gcttcaagat cagtaccctc tttgcttaca gattgatttt cagcatcgga
     2941 ttcactattt atttcatcgg gctctgttga ctttttgtct gtgttttcga gtatttcttt
     3001 tttctctgct tccggcagtt tattcgtttt ttccttttct aattgttttt cagtaaatac
     3061 ttcagttttt tctgtgatat ccgtttttac gtcttttatt tttactgttt ctttacctgt
     3121 ggcgttttct ctatcagagt ctttttcagt ttcttgttcc ttttctggct ctactttgtt
     3181 ttcctgtgca attttttcac tgatcatttt gagcagctct ttcgcgtgct cttcttttgt
     3241 atcttcacga tgtgctttga tataagcgga tagtatcgaa ttttcttcct cattatcgac
     3301 gatttctttc agttcagcta ctcttctttt ttgttttgat tcaagcgttt cttcttgatt
     3361 ctttcctgat tcttccgaag tctcatgaac agttgctttc ttctgttcat ttttctctat
     3421 attagttact tgatcgtgag caggtggttc ttcgtcttct ggttctgctt tttcgaataa
     3481 ttccattaca gattctagag gtaaatcttt caattccgac cattcgattg gatcgtttaa
     3541 ttctgtatca ggaaagactg catcttcagt tgatttatcc tgcatagctg catctgtatt
     3601 tgtttcttca attgattctt ttatttgctg atcttctgca gttatttgat gaccgcagtc
     3661 tggacagata gtattctgct ccattttttt cgatccgcaa tttggacatt ttttcatcac
     3721 ttagcgatcg gctcctttac taggtttacg ctaccattta atctatcata ttctatacgt
     3781 cacgccaata gcttattttt aggatttact taaaaataat gaatgaattt ccttcctata
     3841 gaaaaaaaag cttgagactg cgtagtctca agcttttttt cttttaaaat aaaccaaaca
     3901 gttttactga tttctcttct ggtcaatagt ccacgcttat tctaagaaat cttttaattg
     3961 ttttgaacga gaaggatggc gcaattttct taatgcttta gcctcgatct gacgaatacg
     4021 ttcccgagta acaccaaata ctttccctac ttcttccaat gttctcgtcc ggccatcatc
     4081 taaaccaaaa cgtaagcgaa ggacattctc ttcccgatca gtcaatgtat ctagtacatc
     4141 ttccaattgt tcttttagta actcataagc tgcgtgttct gctggacttg ttgcttcctg
     4201 gtcttctatg aaatcaccta agtgagagtc atcttcttca ccgattggcg tttcaaggga
     4261 aacaggttct tgcgcgattt tcaagatttc tcggactttt tctgttggaa gatccatttc
     4321 cgcaccgatt tcttctggcg taggttctct tccaaggtct tgcagcaatt gacgttggat
     4381 acgtatcaat ttattaattg tttcaaccat atgaactgga atacggatcg tacgtgcttg
     4441 gtcagcgatt gctcgagtga tggcttgacg aatccaccat gtagcatagg ttgaaaactt
     4501 aaatcctttg cggtagtcaa atttttcaac tgccttcatc agtcccatgt ttccttcttg
     4561 gatcaagtca aggaactgca tgcctcgtcc tacataacgt ttagcgatac ttactactaa
     4621 acgaaggttt gcttctgcca gacgctgttt cgcttcttgg tcgccttctt cgattttcaa
     4681 tgctaacgct acttcttctt ccgcagttaa aagggagaca cggccaattt ctttcagata
     4741 catacgaacc gggtcattga tttttacacc ggttggcgca gaaagatctt ctgtttttgc
     4801 ttgttcagct tttttctcag cactcttcaa gctgtgaatt gatggatcac cattttcatc
     4861 caccacactg atacctgcgt cttcaatctt ttggatcaat ttgtccattt catctgcatt
     4921 caaagtgaat ggtgtagcaa gctgattgga taaatcatca tatacaactt gtcgtttggg
     4981 tttgttttct ttgatgaaag ctgctactgc tgcttcatac gtttttttat ctgttccatt
     5041 tgccataaaa gaaggccccc cttgagtagc tatttggctt gttttaactg tttcgttaaa
     5101 ttgatgattt caatcgtcaa ttcaagctca agctgtttgt ttccagttcg tctggcttct
     5161 tgctgcattt gtttttttaa tgttatttca tcagctaagc cagatttgct gatcacatct
     5221 agcaagtctt ggatttctcg tggcgaactt tcttctgcca ccggtaaata ttcgatttcc
     5281 accaccaacc gccttaaatt ttcatcttgc aaaaagtcta aaaactctgc aaggttgaat
     5341 tcataatgtt gtgagacata tgcatcgaat aaaaaataaa cttctgcata tgcatcatga
     5401 ataaactgaa attctcgttc ttttaaaata ttacggacag aaacttcttt aaatgcacga
     5461 tataatagaa tctgctctgc cttttctact tgcgtcaaag gtctcttttt ccgtgcagtt
     5521 tcttgtctcg taacctgagt gtgttgactt tcctgaggtt cttgacgatt ttgctgattt
     5581 gtccttcgaa gatcgcgcag ttgtttttgt atcgtctctc tgccgatctg aaactctgtt
     5641 gacagttgag atagatacat atcttgttcg attggtgaaa caactcggct aagttcaact
     5701 agtaattctt cgaggtattc gatcttgtct ttttcattat ctaaattttt cccttgtttt
     5761 aaataacgtg atttaaaagt aaagactgtt tcgcgcccat gagccactaa ttcttggaaa
     5821 gatgcttctc catacttacg gagatattca tcaggatcaa gcttttctgg aacgactacg
     5881 acagaaagtg ctaatcgact gttttgccga agcaattcaa tccctcgatt ggttgcttca
     5941 aaacctgcgt tgtctccgtc atagcaaaag accaattctt tcgtcagctt ttccatttgt
     6001 tggatctgtt gagctgttag acttgtcccc atagaagcta atccagtttt gataccagcc
     6061 tgccatgcag caatcacatc cataaaccct tcgaacaaca aaatttctcc gtttttccgg
     6121 atatcgcttc ttgctttatc aagattaaat aaaacttgtc ttttattgaa cagttctgtt
     6181 tctggactat tcaagtattt gggctgatct ttttcctgcc ctttttcagg tgcaagccag
     6241 cgtcctgaaa aaccgatcgt ctggccttga gaattacgaa gtggaaacat cactctttga
     6301 taaaaacgat cagacatacg gccatcatca tgcgtgacaa acaatccaga gcgttctaac
     6361 aattcatttg aaattgattc attcttgaat acttgctgaa gaaaagaacg ctcattaggt
     6421 gcaaaaccta tgttgaattc ttcaattaat tctaaagaca acccacgact ttgcaaatat
     6481 tctaaagctg gctgtccagc ttttgtatgc aatagcatgt gatgatagat ttccgctgct
     6541 ttttcatgag cagcaatcag tttccccgta gcagattcag gattttggac agctatagta
     6601 cgctgacgcc attgatcttc taacggtatc tgttcgaagt ctgccacttt taccactgct
     6661 tctggaaaac ttagaccttc tagttcttga agaaaagtaa acacgtttcc gcctttacca
     6721 caaccaaagc aatgaaaaat ctgcttgtct tctgcaacag aaaaagacgg tgtacgttct
     6781 tcatgaaaag gacacagccc caagtaattt ttaccagact ttttcaactg gacatattgt
     6841 ccaatcacct caacgatatt cgtttgactg cgtactgttt cgataacctc ttgaggaatc
     6901 cgttgagcca ttttttcacc ccctgctcta ttgatatctt ttgggtttaa cagaagatat
     6961 ttcaggagaa taaccggtaa aaaatcgcac taactgaaat cagtgcgatt ttaaaggaca
     7021 caattataca tgtttgattg tatcatattt cattgctttt tcaagtataa cacatttcta
     7081 tctcattaag catcaatttg ctactctttt cagtaactga ttttgaatat cagccttcca
     7141 gtacaatact cgaagaacaa tatttacgag catgaataca aagaagtatc ctacgctctc
     7201 tcccaaccct aagtttactt ccaaaattcc atggaacagc attgcagcta catatatccc
     7261 tacgatcgtc gttactgcta ggaagatgcg caatgggttg aagggataac aagcttttac
     7321 aactgccatg caactgatcc caatcagcaa gtaatacatc aaagtagtag tttcagcatt
     7381 tccccatgcg aatgtttttc caagtagata caccgcaatg atattcacta ctacaagcaa
     7441 ggcatttggt aaagcattaa taagtgctgt aggcaaataa cggtaagtga tttttctttt
     7501 atcttgttca aaagataaaa agaaggaagg atacccttca atcgctaaat caatcagcgt
     7561 gatttggata gggataaacg gaaatccaat tgctgtaacg gcacatataa tcgataaaat
     7621 aaatgaataa atcgttttga taaaaaagat gcctgagact ttcgtcacat tgttgaccac
     7681 tcggcgtcct tcaaacagga cttctggcaa tgtagtaaaa tcagagtcca acaataccag
     7741 attcgcaatc tgacgtgttg ctccatcacc ttctgccata gcaatgctgc aatctgcttc
     7801 tcttaacgcc aaaacatcat tcacaccgtc gcctgtcatt gcaaccgttc tgccgctttc
     7861 tttcaattca tttaccagca atttcttttg ctgcggactc actcgtccaa aaaccgtata
     7921 gcgatgagca gcttctcgca cttccatttc ttcagtaatt tgagaaagat cgacatacgc
     7981 atcataattc ggtaatcctg cacgtctagc gatattggag acagtaaaag gattgtctcc
     8041 agaaatcact ttcagatcca caccttcctc atgaagataa gctagtgttt cttgagcgtt
     8101 ttttcggatt ggatcatcga tttcaaacag agcaaccggc tcaacaaatg ctggtttttc
     8161 ttcgttcaat tgctgatttg gagcgatccc caacataagt acgcgatagc cattcgcttg
     8221 ggctgttttt agactgtccg gaagccgatc ttcttccacc aaacgttcag gcgctcctac
     8281 gtacacgact cccagttctg gaaattccat cgcgccccat ttgcgatcag aagaaaatgc
     8341 gactttgttt gtcacatcat accgattaga aagctcaaaa taatctctca tagcccgcat
     8401 agtgatgttg ttatcactgc tttctgccag atacgtcccc ataatttctg ggatggcttt
     8461 ctcgtacagc ggatgtagtg cttctacccg ctggatactc attttccctt cagtaatggt
     8521 ccctgtttta tccaagcata atgtatcgac atgggccagc gtttcaattg aatacatgtc
     8581 ttgaacaagg attcgttttt ttgccagttt ggttacccct gtcgttaatg caatacttat
     8641 tagcaatacc aaaccctttg gaagcatgcc taacaaagct gctgcagatg caataacaga
     8701 aattttgata gaagcatcgc ggatgaacag tgcttccaaa aatagtatga tccccaaagg
     8761 aataatcacg aaactagtga atttggatac tttgcgaatc gaatctacaa gttcagaacg
     8821 aatagctttg tgagtctttg cctcgctagt caagcggaca gcataattat ctgcacctac
     8881 tcggataact tcagccatga cagaaccgct tgtcaaaaag cttccagaaa gcaattcagc
     8941 tccagtttct ttgacaatca aatcagattc gcctgtcaaa agagcttcat ttgcttctgc
     9001 ccgtccagat ataacctgta aatcggctgg tatctgttct cctgctccga tttttaccaa
     9061 atctcctaat accagctctt tagtagaaat cgattgttct ttaccgtccc ggatcactag
     9121 tacttctgat tgggaaacaa tcgataattt ccgtacaaga tttcttgcat gtatttcttg
     9181 ataaattcca ataacgatat tgacgacaat aattgctaaa tacgccatgt tggaatacgc
     9241 acctacaaat aacagacaaa tcccaatacc aaagttcaga aaattgaata acgtcaatat
     9301 attatcgctg aatatttgtt ttgtactttt tgatgcatct tcctggtagt cattttgctg
     9361 gccagaagcc attttctgaa ctacctcttg ctgtgtaagt ccattcattt tctttttctc
     9421 cattttccgc caactttcaa aaataatcac gtgtttaagt ctagcgtgtt ccaagattaa
     9481 aaacaacttg tttttataaa atttatagaa aaacacacga atgattttac aaaaaagatg
     9541 ccaacctttt tgtttgaatc acccgtgtgc tgtttttatt atcaatctat ctttacttat
     9601 attgttcctc caaacggctc atccaccaac ctagtgctgt accggctata aaaagaacag
     9661 gacaagctag taccccagca actcccaatc ccgcgtaatc ggtaggaatg atcataatcg
     9721 ttgaagcaaa aactacacct aaaatgaaat ggaatagctt gctatatgcc tttgagaaaa
     9781 tgtaatccat gatttttgac aatccaagaa cacaaagaat cccaccaatt ccgatcggca
     9841 caatcacttc aagtcgtact tctttaaatc cgtcagccat cgctttatac aatcccatat
     9901 acaccaaaaa attagaaggg ctcaatcctg ggacgatcat acccaaacca atcaatccgc
     9961 cggcaatcag ccatgttcca aaattctgat cgacttgccc gtcaaataat tgagcgccgc
    10021 ataataaaaa caaataagcc agaacaaaag taacaaccat gatgatcaca tcagaagtat
    10081 ctcttccttt tttccctgct tctttccata acgcaggaac agttccagcg atacatccga
    10141 caaaaaacca taatatcgtt gtttcatacg cacctaataa aaaactgact aaaaaagaaa
    10201 gtagaaagat gcctgtcact ccgcctagac caacaggcag aaaaaacaaa acattttctt
    10261 tgaaattttt tgtaaggtgg gctaaaaaag aaatcagccg ctcatacatg ccaaagacag
    10321 ctgctaatgc accaccgctg actcctggca agataaatcc tgaaccgata aacattccct
    10381 tcacaaaccg taacatccag tctttggttg tgtaccccac ttcttgattt tccatttttt
    10441 gccttttttc cactatttgc gcactccttc tttcatttaa ttttattcat gattgaacat
    10501 ttcctgctat tttacttaaa gactttttca taggtctcta ttattttttc attcattttg
    10561 acaatatcag gataaaacat gttacctcca ttatgataga gatagccgcc attgtaaagc
    10621 aaggactgga tccgccaata tcggtattgt ttcccttcct catttcctaa aagtgggctc
    10681 agcacatctt tagaatacgc ttctgctaat tctactgtgt tttctccgcc atgatttttt
    10741 acataggcaa tataatcttt cccaaaatta tatgcttgga cagctgtcca taaatcgcat
    10801 ccttgtttct ttgcttctac tatcatttcc gccaaatatt tgacaccttg atcaatactg
    10861 tcagctggat tatcaatgat ctcagcttct ccatgcagac tttccgagct ctgcatgaga
    10921 tctgtccctt ttccttttga ttcggtcaat attatcgcct tgatcaaatt ctcgtagttt
    10981 tccatatgat aattttttgc agctgttgtt atttctattt gatagctttc cactcgtttt
    11041 accgaacgat aagttcttcc tcataaaaac aacaccgcac caataaccgt cagaatgatc
    11101 atgtaaaaaa gaatacggat aatagataat cgttttcgct tcatttcatc acctagttat
    11161 tttgacatct ttttttatct tactatttct ttatcttctt ggctatttct tccttgattt
    11221 ctgtgtacgc cttaagaaaa aatgaatttt cctgtatttt ttgacatctt acgaaaatga
    11281 tcgttgtttt tcagtaaaaa caattgccca atgactgata taatcgttat actttacaca
    11341 gtaggtgatt atgtgaaaaa agaaaagaaa tatgcaaaaa tgccagatgg gagcgaaatt
    11401 tactatgaaa agagcggtca aggcttccct ctctttttac tgcatggcaa tgacggcagc
    11461 ggccgctttt tttcagaaca ggtccctgta ctcgaacgct actataccgt gtatctagta
    11521 gatagccgag gacatggtcg ttcgacaaac gaagcatcta tgttgaattt tcagttaatg
    11581 gccgaggatc tgaatacgat catgttgctg gaaaaaatcg atcaggcaga ttttcttgga
    11641 tttagtgatg gagcaaatct agcactggta tttgccagca gcttccctaa aaaagttcat
    11701 cgattgattt taaattctgg aaacacgctt gttaaaggcg ttcgtttttc tgccagagtc
    11761 atcagtaata ttcattatgc atgggtatgg ttactctctt tatttcgccc aagtcttcgc
    11821 aaaaatcttt tagttatcaa acttttgctg catgacatcg ggcttacaga aaatgattta
    11881 aaaaaaatca actcccccac tttgatcatc gtgggaaaaa aagatgtaat caagctaaaa
    11941 cattctttgt atatagctaa aactatacca aaagcttcct ttgtgcttgt gaaagaacaa
    12001 ggacatgaat tagctagaaa agatcctgaa cgcttcaatc gagaagttct tcaatttctt
    12061 tctgaaacat aactattagg aatagaaggt gatttatttg ttaaaaaata ccatacaatg
    12121 gctgaaaaac cacctaggac tgttcaaaac aatctttttg atttctgtca ttgtcattat
    12181 tgtcggtgaa ttgatgtcca tcggtaaaac tctatctatc cagcaattag gagaaacgtt
    12241 tgccactata cctgtatgga aaagcggact aatgctattg attggtctta tctctgtgtt
    12301 acctatggtt ggttatgata ttattttgaa tcggatgctt gaacaaaagc agaatcctcg
    12361 ttacctgttt gagacaagct ggctgatcaa tacgatcaac aatattgccg gtttcggcgg
    12421 gtttgttagt gtggggcttc gctctgaact atatggacaa aagaaagaga gcagaaacgt
    12481 gatccaagca ttatcgaaag ttttcttgtt tttgatggct ggactttcta tttacagtct
    12541 gctttctttt ttacttgtag tcttcacgcc agttgctcct tttttgaagc aatattggat
    12601 atggttgatc ggcggcggtc tatattttcc tgtcgtcttt ttatttacta ctttcaaaaa
    12661 gaaaggattc cttggtggta cctcacttaa aactcaagga cagctattgc tagtttcatt
    12721 gttggaatgg accggtgtcc tgtttagttt cctttctgtt ggttatttaa tggaaatccg
    12781 gattgatctg tggcaaacaa ttccattatt cattgctgct tcagtgatag gaatcgtgtc
    12841 tatgattcca ggagaaattg ggagtttcga tgtaatgatg attatcgggt tgtccgctat
    12901 tggtgtgcca agagaaatag tcgttgtgtg gatcttgctc tatcgcctgt tctactacat
    12961 cattccgttt ttgattggaa tcgtcttttt cttcaaaaat atcggttcca cttttgatca
    13021 gcgttattct ggtatcccta aacaattagc aacagaaatt gcgcataaaa tcgtggttgt
    13081 cttattatac ttttctggaa tcatgctagt actttctgcc actatcccgc aagcttttac
    13141 agagtttcgc tggcttcata gtttgaaccc attgaaattt cattttatta tccaatttcc
    13201 tagtatacta ttgggatttc ttttaatcat tatgggcaga gggatcgcag cacgtgtgaa
    13261 acgagcatat ttgcctacaa tcttcttgat tgttctagct ttactttatg ttttattaag
    13321 tgattttagt ttcacaccgg tggttttctt gtctattctg cttctgatca ttcttgcttc
    13381 taaaaacgaa ttatttcggg aacaattgat ctattcatgg gagtggcgta cgatcgatgg
    13441 aatcattatt ggtgcactgt ccttgcttta catcgttatt ggggtataca atctgccgga
    13501 tttccctcat cggcgtcacc attttatttc ctttttctta tttccttctg aaaagatttg
    13561 gttttcggga ttgttggcca tcatcgctgt tagtttcatg atcgttctat ttgttcattt
    13621 ccttcaagga gaaaagaaac agattggcga agcctttaat gaagaaaagg ccctgaagat
    13681 ccttacaact tatggaggaa actccgatag tcaattgatc ttcttaaaag ataagcggat
    13741 gtttgcctat gaaaaagacg gtgaaccaac tgttcttctc caattcgctt gcttcaataa
    13801 taaatgtatt gtaatgggtg atccttctgg taagaaagaa gattttccag aagcaatcga
    13861 agcttttatc gaagaaacag atcgactatg ctatttacca gttttctacg aaacaagcga
    13921 agagattgtt atgattttgc atgaattcgg ctacgatttc attaagatgg gggaagaagc
    13981 ttatgtagac ctgaatagtt ttacaacttc agggaaaaaa atgaagggca ctcgtgctgt
    14041 cttgaatcgg atcgaacgag aaggatttac atttgatgtc ttacaaccac cattttctac
    14101 tgagcaaatg agtactttca aaaatatctc tgataattgg ttgggttcac gaaaggaaaa
    14161 agggttttct ttaggctttt tctctgaaga ttatctacaa agagcgccta ttgcagttgt
    14221 caaaaatacc caagaagaaa ttgtcgcttt tgcaacgatc atgcctactt acacgaacaa
    14281 taaagtagga acgattgatc tgatgcgata tgattctaaa aaagcacctt ctggcagtat
    14341 ggatttcttg ttcttgcatt tgtttgccta tatgaaagaa gaacatatcc aatggttcaa
    14401 tttagggatg gcccctcttt caaatgtcgg aacttccaga aagagttttt tacaggaaag
    14461 gattgcatac ttggtttacg agtttggttc tcatttttat tctttccatg gattaagaga
    14521 atataaaagc aagtacgcta caaactgggt ctcacgttat actctatatt ctagagatag
    14581 ttggattgca tatgttatga ttgctttgct gattattgat aatgcgccag ttgagcagac
    14641 acaaggtaga ataagtggtt tgaaaaaatg gatacgaaga aagtattaaa aactgcatac
    14701 cgatagatga acactctcat gatgaataaa agagactgaa gtaaaacgaa agttcatgct
    14761 tctacaatct cttcttatac aattgaagaa caaagagcgg tccaaacaga aatcctgttt
    14821 ggaccgctct tttattttaa acatttttca ttaccaaaat attcgagtcg cgtttcttat
    14881 gcttcgatct ctgtaacgat acctgaacct actgtccgtc cgccttcacg aattgagaat
    14941 gtcgttccgt tttcaacagc gattggatgg atcaagtcaa catcaatcgt cacgttgtca
    15001 ccaggcatga ccatttctgt atcttctggc aatgtgattg ttcctgtaac atcagttgtg
    15061 cggaagtaga actgtggacg atagttgttg aagaatggag tatgacgtcc accttcttct
    15121 tttgtcaaca cgtacacttc tgctttgaat tttgtatgtg gagtaatgga acctggttta
    15181 gcaagtactt gtccacgttc gatatcgtca cgttggatcc cccgtaacaa cacgcctacg
    15241 ttatccccag cttccccgta atctaacgtt ttacggaaca tttctacacc tgttacaact
    15301 gctttttgtg tttcaggttt gatccctacg atctctacct catcaccgac acgaacagca
    15361 ccacgatcaa tacgacctga ggcaacagtt cctcgacctg taattgagaa gacgtcttcc
    15421 actggcaata gtaatggttt atctgtatca cgttctggtg ttgggatata ttcatctact
    15481 gtatccatca attccataat agcagcttca gcatctggat cgccttgcaa tgcttttaat
    15541 gcggaacctt tgatcacagg agtgtcatcg cctgggaaac catattcgct caataactcg
    15601 cgaacttcca tttctaccaa atcgatcaat tcttcatcat cgactaaatc aactttgttc
    15661 aagaaaacaa tcaggtattt tacaccaact tggcgtgaca ataaaatgtg ttcacgtgtt
    15721 tgaggcatcg gaccgtcagt tgcagaaaca actaagattg caccatccat ttgtgctgcc
    15781 cctgtaatca tgtttttcac atagtccgcg tgtcctggtg catcaatatg cgcgtagtgg
    15841 cgtttttctg tttcatattc tacgtgtgcg gtattgatcg taatcccacg ttcacgttct
    15901 tctggagctg catcaatact agcatagtct tgaggatttg ctaggccttt tttgcctaat
    15961 actgtagtga tcgcagcagt taaagtagtt ttcccatggt caacgtgtcc aatcgtacca
    16021 atgttaacgt gtggtttact tctgtcataa tgttcttttg ccataattta caaacctcct
    16081 aaataatata gatcactgaa aaatttcagc tctcaccttt ttattatagt ctttttttga
    16141 ctaaatcaaa aagaatacgt ccgacttact ttttgtaaga ttcgaactaa gcttattata
    16201 cgtcaatatt ggtcaaagtc aactaagagc actcgtctgt cttatacgcg aaaaaagtac
    16261 cttttataaa taaaaaaatg tgttttttga gaaaaacttc aagatagtcc ttaactttct
    16321 tttgaagcaa taagaacaaa atgaggaaaa gtttaaaatt cagctgttgc caacaacttt
    16381 caatagacga aatcttctat ttcgttaata ataaggaaag aggtgaagta catgaaaaaa
    16441 ttaattggcg tacttgtcgt tttaggcata tttctcggcg gaagttttgc agcttatcat
    16501 tatttttacg gcggcgaagc ttattataca aaaatcacga cgaatggcga aatgtcagct
    16561 acccaagcag ataacggaga gaaattcacg acttatacct acaagcaagc ggcttataat
    16621 aaagatggtg aaaaaaaaca ggtgacgctt cgcgaagaaa gagaacatcc attaaagatg
    16681 aatgcgtatc ttaaactcaa agtcaatcct cgtaaaggtg tattaagctg ggaagaaata
    16741 aaggcttctg aagtgcctaa aaatgcggca gaaaaaatcg ataattagta aatggtctta
    16801 aaattttatt agataaaaag aggctgggac agaagcgttt aactccaaga aata
//
LOCUS       DACECR010000063        16589 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.063, whole genome shotgun sequence.
ACCESSION   DACECR010000063 DACECR010000000
VERSION     DACECR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.063"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(81..788)
                     /locus_tag="IWO17_001875"
     CDS             complement(81..788)
                     /locus_tag="IWO17_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ0290743.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(867..1205)
                     /locus_tag="IWO17_001876"
     CDS             complement(867..1205)
                     /locus_tag="IWO17_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ0290744.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1316..2278)
                     /locus_tag="IWO17_001877"
     CDS             complement(1316..2278)
                     /locus_tag="IWO17_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ0290745.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2293..3624)
                     /locus_tag="IWO17_001878"
     CDS             complement(2293..3624)
                     /locus_tag="IWO17_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0290746.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3632..4312)
                     /locus_tag="IWO17_001879"
     CDS             complement(3632..4312)
                     /locus_tag="IWO17_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ0290747.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4531..5010)
                     /locus_tag="IWO17_001880"
     CDS             complement(4531..5010)
                     /locus_tag="IWO17_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ0290748.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5137..6816)
                     /locus_tag="IWO17_001881"
     CDS             complement(5137..6816)
                     /locus_tag="IWO17_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0290749.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7364..8866
                     /locus_tag="IWO17_001882"
     CDS             7364..8866
                     /locus_tag="IWO17_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0290750.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8879..9541
                     /locus_tag="IWO17_001883"
     CDS             8879..9541
                     /locus_tag="IWO17_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0290751.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9642..9845)
                     /locus_tag="IWO17_001884"
     CDS             complement(9642..9845)
                     /locus_tag="IWO17_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0290752.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(9956..11140)
                     /gene="iadA"
                     /locus_tag="IWO17_001885"
     CDS             complement(9956..11140)
                     /gene="iadA"
                     /locus_tag="IWO17_001885"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ0290753.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11297..13036)
                     /gene="yfcC"
                     /locus_tag="IWO17_001886"
     CDS             complement(11297..13036)
                     /gene="yfcC"
                     /locus_tag="IWO17_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0290754.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13809..14423)
                     /locus_tag="IWO17_001887"
     CDS             complement(13809..14423)
                     /locus_tag="IWO17_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0290755.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15029..15406)
                     /locus_tag="IWO17_001888"
     CDS             complement(15029..15406)
                     /locus_tag="IWO17_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290756.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15706..16587)
                     /gene="galU"
                     /locus_tag="IWO17_001889"
     CDS             complement(15706..16587)
                     /gene="galU"
                     /locus_tag="IWO17_001889"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0290757.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4809 a         3573 c         2831 g         5376 t
ORIGIN      
        1 tattccaaat aaatggtatt caatcagcta actctcggta ttagctcaga tcaaagcgct
       61 gatttcacaa cttctttatt ttaatctctt cggttgccgc cgaacaataa gatcaagcgc
      121 aacagctgca ggaatgtcga aagcgctgct gcaacataag tcagtgctgc tgcaaataat
      181 acgtgtcttg ccatcggtac ttcttcttcc gtcagtagtc cgccttcact caaaatttgg
      241 attgctcgcc gagacgcgtt aaattcgact ggtaatgtca ccaattggaa aagtaatgct
      301 aaagaaaaag cgaaaatccc gatattgatt aacgtagagt tccagctaag caatacacct
      361 accataatca aggggaacga aatcatcgaa ccaaagttag ctgctggaac aagtgctgca
      421 cgaatcttca atgggacata acccgaagca tcttgtaccg catgtccgca ttcatgagct
      481 gctacaccta tcgcagcaac tgaagtagac tgtgcagttg cttgagacaa actcaacatc
      541 ttattacctg aattgtagtt atctgtgaga tctccggcaa tttgttgaac accgacatta
      601 tttatctgtt gactttgtaa tatatattgt gctgcttgtg taccagtcac attgtttttg
      661 cttctcactt gatcgtattt tcggaaagta ctattgacgt acgctgacgc aattccggaa
      721 ataactaatc caagaatgac caaaagaaat gtcggatcta aaaaaccata cattccataa
      781 ccatacattt tcgtttcact ccttttcgtt tactttattt tatcattagt agtaggaaaa
      841 gaatgtgaaa agaccttgaa tattctttaa ttctttttca caaattccga tttcagtttc
      901 atcggtccaa atccttctac cttgcaatcg atattatgat cgccttctac taaacggatg
      961 ttcttgactt ttgttccttg tttaatggca cctgacgcac cttttacttt caagtcttta
     1021 atcactgtca cgctatcgcc ctctaacaga cggtttccat ttgcatctaa taccactaat
     1081 ccgtcttcta tgacagtttc ttgttcttct ttgttccatt cgttcccgca ttctgggcaa
     1141 atcaacaagc ctcgatcttc atatgtgtac tctgagccac attctgggca atttggtaat
     1201 gtcatcttct ttctccttta aaatctttag tgagcactat tgtaacggaa aaagaagatc
     1261 ttgaaaagat acatttgtga tttttactac aaaaggcacg ttttcgaacg aagtatcatt
     1321 ctgtttttct gctcttatac gattggcata tatcttggat ctctttatag atttcctcta
     1381 cttctgctaa atcttttgca ttcgcaccac ttgctaatgg atggcctcct ccatgatggc
     1441 gtttagccaa ttcattgatg atcggcccct tagaacgtaa acgtacgcgg taataccctg
     1501 aaggctgctg aacaaaaatc ccccaagcca gcacttcgtc aatcactcca ggaagtgaaa
     1561 cgatcgaagc tgtctctgaa tcatcgattc catatttatc aagaatttct tgcggaagga
     1621 ttattcttcc tgcaccattt tcatccacct ggatattttg ataaagataa ccggaaagtt
     1681 tagcgacttt catcggcatt tgttcaatct ctctgttcaa agcacttgca tcaaatggat
     1741 aatttctcaa tatcgaagca atctctaatg tacgggacgt cgtggaggga tacaaaaagc
     1801 gccctgtatc tccgacgatt cctgcataaa gtaaccgtgc agccttaacg ttcatcttta
     1861 aagattcagg aaacatcaaa gcaaattctg caatcatctc actacagcta cttgcttttg
     1921 tatttaccca cacaagatct ccataagggt catcattggg atgatggtcg attttgatca
     1981 gtttttctgc caactgatat ctttgatcac tgattctggg tgaattagct gtatcagtca
     2041 cgatgactag cgctccttgg tagaagacat cttcaacttc ttgcatttct gctaaaaagc
     2101 gaagcccttc aatcgtttca cctacttgac gtatttcttt ttctgggaaa ctttcccgta
     2161 atagttcggc taatcctact tgtgatccta atgcatcagg atcagggcgc aaatgcctgt
     2221 ggataatgat tttatcgtat ttttttattg tatctactat ttcttgctgt actgtcattt
     2281 cactcctgct ttctaagaac gttccattac ttgacaaaca acgattgctt tggaaacgat
     2341 ggtgttttca ataaacactt cgatatccag tttagcggaa cgtctgccga tttccaatac
     2401 acgtggacga atgtctaatt cgctttccaa ttggatcagc cgcaaataat gtaggttcac
     2461 ttgttcaatc aagatatttc gtttttggtt catcaccatt gttttttgtg cgacatcaga
     2521 aatgatttcg ctaagtacgc caaaagaaat ggttccaacg ccattgacca tttgtggtgc
     2581 tactacgaat ttgaacttag ggttttccaa acgatttcct tcacggtcag tatcaatcgt
     2641 atggatattt ccagatatct gatccgaaat cgtatctgca atctgcggtt gtcgctggac
     2701 aagctgcatc gctttcatta catcttgccg ggtaatcaag ccttccaatg tcaagtcatc
     2761 ggaaacgacc ggcatgactt ctagcccatc ccagatcatt tgatggctaa cagaggcaat
     2821 actcattgtt ttctttacac tgattggttc tttcgtcatc acccgatcga tgatctggtt
     2881 tggcgatttc cctaaaacat cttttgccgt aacaatcccc atcaaacgat gatggcggtt
     2941 gacgactgga aaacgcgaat gctgcgtttc ttctgacaaa cgctgataat cagaaatcgt
     3001 atctgtctgc tgcaaataat gagtcttttc agctggcata tagatatcac tgacaagcat
     3061 gatatctttt ttgatcaatt ggtcgcttaa cgctcggttg atcatggttg cgactgtaaa
     3121 tgtatcataa gaggtgcgta agatcggcat ttcaagatca tcagctagct ttgcaatctc
     3181 agcagtcgta ttgaatcctc ctgtgatcaa tactgcggcg ccatcttcca atgctaattt
     3241 ttggacttcc gcccggttgc cgacgatcat tagtgatcct ggcgtaatat agcgttccat
     3301 ggcatgttta gtcatcgcgc cgatgacgaa tttattcaag accttgtcta atcctgccgc
     3361 cccgcctaaa acgtcccctt caatgatcct gacgacctcg ccaaatgtca aacgttcaat
     3421 atgttttttt aatttgcgtt cgatacggat ggtccctact cgttgaattg ttgaaactag
     3481 tccgacattt tctgcatctt tgattgcacg gtacgctgtt ccttcactga cattcaattc
     3541 ttttgcgatg cttcttacag aaattcgatt gccaacaggt aaactttcaa tatattctaa
     3601 aatcaaatca tgttttgtcg ccattttctg cctacacttc cagcgtttcc gagacgctca
     3661 ttatttttcc ttcactatct tccagtaact ctacaaaaga ttcgggatct tgttcaatga
     3721 cagggaaagt attgtaatgg attggaacta ctttcttggc acgaattgtt tgcgcagcct
     3781 ttgctgcatc ttctggcccc atcgtatagt tatcgccaat cggcaagaaa gcgacatcaa
     3841 tatcgaattc ttctgcaagc aatcctagat cactgaaata agcagtatcc cctgcatgat
     3901 aaatcgtttt tccttctgct tgaataataa ttccgctagg ctcgcccata tactgaacga
     3961 taccgtctac ttcatagctt gaactatgct gtgcatgtac gaattttacc acgccgaatg
     4021 gaaacgcatg ttttccaccg atattcatac cgaatgaatc tactcctctg ctgttcgcat
     4081 aatcagcaat ctcaacaata ctgataattg tggcttgatt ttcttttgca ataggaatca
     4141 tatctcctat gtggtcacta tgaccatgag taatcagcaa ccaatctgtc tttacttctt
     4201 ctgcagttaa gtctgtcaat ggatttcctg taataaacgg gtcaatcatc acgcgttggc
     4261 cattttccat ttcaattgtt aaacaggaat gtccatgata cgtaattttc atattttaca
     4321 cctcgcgttt tctctgtatc ttttttatta aaaacgattt catcacttgc acatcaatga
     4381 aaaaatatag ctgctaaata tattttcatt ggattgatag tacagtatgt acaaagcaat
     4441 tgtaacagat aatacaggat gcaccaagta ccaagcagat ataatctcaa gtttgaggtc
     4501 tctgctctgc ttagcttctc ttctcgtccc ttaactaatt aaatcagcgt aaataggatg
     4561 gacgatcttt tctaaatcgg ctacttttat ccgaaggctt ctcccgattt caccgcttga
     4621 cacaattact tcttcttttt cttttaaaga actgtctgct aaaaaacaat aatcaggatg
     4681 tgcgagtttt attccaatag gggtgtttgc tccgtgggga tatcctgtat gcattaatac
     4741 aaaatccatt ccaggaaaac caactttacg atctttcgtc acttgtctcg tttttttgta
     4801 atctaagtga tcgttcaaag gaatcaatgc gatgatcacc ccagttttgt ttcctttcac
     4861 tacgagagtt ttacatatca aagctggatc aatccctctt ttttgtagtt cttctgtgaa
     4921 agattcttct accacatgac taaaatcaag tggttgatag ctgatctttt gttcttctaa
     4981 ataacgttcg acttttgttt gactcatcat tgattcactc cctttattct atcgtttatt
     5041 ataaagcgag ggatgctgta ttcaaaaatt atttgttttt cgcaaaaaaa gagcaagacg
     5101 agcactctat agagaggttc atcttactct ttttgtttat tttttatttc gacgttgagt
     5161 agcttccata atgactggca agatcactgg gcggcgtttt gtttgttcaa ataagtaacg
     5221 gcctaattga tctcggatgt cttgctttaa tttgctccac tcaaaatcat tgctttctaa
     5281 atgtttctca acgattgttt caatcatatt gctgctttct ttcatcagat cacggctagc
     5341 ttttacgtaa acgaatcccc gtgaagtaat ttgaggtttt gaaatgattt tcttttctcg
     5401 ccgactgata gtgacaaccg ctacgaaaat cccgtcttct gacaagattt ttcgatcacg
     5461 taaaacaatg tttccgatat cacctatgcc taatccatca atcattacat tttctgcact
     5521 tgtagttcct gcaacactca tacgcttgtt cttatattcc agcacgtctc cgcgtccagt
     5581 aatatagata tttttgtatg gcatccctac tgcatgagcc aaatctgcat gagcagctaa
     5641 ttcacgatac tctccttgaa ctggcacaaa atatgttggt ttcatcaaat tgatcattaa
     5701 ttgcaagtca ttaggattgg catggccaga aacgcgcata ttctcagaga tcaacttcac
     5761 tgttccacca gctcgataaa tgatatcttc cgttttcgcc acgatggttt ccatcgcgat
     5821 gcttggcgta gtagtgatat aaaccaaatc tccttcttca atcttgacat tacggtgtgt
     5881 accgtttgcc attttttgca gagacttgat gggttcaccc attcggccag tttctaaaat
     5941 gatcagttct tctggatcat agtttttcat gtctttcaac gtaacaagca gatcttcact
     6001 tggtaattga agtttttcta aacgcatcgc tgtgcgaata attcgttcga aatcttgacc
     6061 agtcaagaca acttttcgtt gtgctttttc tgctgcatcc aatacttgtt gcactcgttg
     6121 caaattactt gctacacaag cgacgatgat acgcccttcc caatatttga tcgtatcaaa
     6181 tacttcatct gctatttggg cttcagaagc tacaggctga ggattttctg catttgccga
     6241 ttcacttagt aaggctaaga ctccttcttt gccgatttcc gccaatcgtg cataatctgt
     6301 ttgatacatt gggatagccg tttggtcaaa tttaaaatct cccgtataaa cgatactacc
     6361 ttcttttgtt ttcagattga tccccactga atctggtatc gtatgagtgg tacggaaaaa
     6421 gctcactact gtttcaccaa agtctatttc tgtatgctca tctaccacat ggaaatcctt
     6481 aaatcctttg gctgcttcat tacgactgac attcaatttt gctaactcga tcgtcaactc
     6541 tgtcccgaat actggtacag aaatttttga caacaagtaa ggaagtgccc cgatcgcatc
     6601 tgcatgtcca tgtgttaaaa agacaccagc tacacgatca atgttttctt ctaaatacgt
     6661 aaaatcaggg attacgacgt cgatccccaa caactcattt tcaggatatt ttagcccaca
     6721 atctaaaata aaaatatcct cgcccacttc tgcgacatac atgttttttc cattttcacg
     6781 aacgccgcct aatgggataa ttttaatggt actcacaaac ttcacctctt cttatgacat
     6841 ccaagtttaa agatgtcgat tttcttcttt atctaaatat atgagccatt ccaatgaagc
     6901 atatgcaaaa tcactatttc tcaacttatg gagaatcagc aggaaaatta ccagcacatc
     6961 cgcgttctga cagttggatg ggcattttat tttggatata cttcattaac aaaatggatc
     7021 ttttgcgaaa cgctatagcc agaatactta tataagtatt cagctgttcc ccattattat
     7081 acaacaaata aactaaatcg ctagtatcag caattgttag aacaactatg cacgtaaaaa
     7141 tccctctgta gcatctagga tttcttttgc tattcctctt tcatttcttc ttcgtcggtt
     7201 tgaaacatac ttttttcatt ttaccacatt ttttgcaata actatagtga ctttttatat
     7261 atacagaacc tcatgttgat ggtgtgcaaa cagcataaga tttaagctag ctttaagata
     7321 ggttgtctat tatcaaatgc atctactagg agggatgcaa aatatgaatt ttatgaaacg
     7381 tgcatggaaa agtacaaaag taaaatgggg acgttcattg ttattgtttg ccgtgttcac
     7441 agccattctt atttttgtgc tagctggcct cacgattcga agcgctgcag aagaagcagc
     7501 gaatcaagca caaaaagaag ttggagcaac agtgacttta agtgccaatc gagaagcttc
     7561 atttaaaaaa caggaacaaa cttcttcaag cggttctacc actgaagaac gtccagatcc
     7621 aggcagtttt tctcttacac cagtttcctt aacggacgcg gaaaaaatag ctgaattaga
     7681 taatgtaaag agttattcct ttgaaagttc cgcctcagct atggcaaaag atggaataac
     7741 tgccatcact agttctgatt caagtgaaga cagttcagaa actactgatg caggcggtga
     7801 acctgctgga ggaatgggcg ggaatccgca aatgactcaa gcagactttc aaatcagtgg
     7861 tgtgtcagca actgcacaaa atagtaattt ttctgatgga acagcaaaaa tcacggatgg
     7921 tgaaggaata gatgacagcg acaaaggcac aaacaatgta gtcattgatt ctactctagc
     7981 agaaacaaat gacctggcag tcggagatac tttcacaatc acgagtactg aagatgaaga
     8041 taccacttat gaaatgacga tcaaaggaat ttatgaatcc agcgaaacaa gcggcagtat
     8101 gggtatgaaa ttcaacttca tgaatccgtc gaatacactt tacacttctt acacttttgc
     8161 aaatgaacta aatgggacat ctgacgataa cacgatagat tctgctattt acactctatc
     8221 tgatccagat aaaatggatg agtttctcaa agaagctgag aagctgatcg acacagatac
     8281 atttagctta caatcaaatg attccatgta tcagtcaatg ttggaacctt taaacaacgt
     8341 ggcaagtttt tctaaaaatg ttgttttact agtgactgta gccggtatta tcatacttac
     8401 tctgatcatt atgattacca ttcgggagcg tcggcatgag ttaggcgtct tgctatcttt
     8461 aggagaatcc agaagtaaaa tcattctcca attgtttacc gaagtggcta tttgtatgat
     8521 tcttgcatta ggagttgctt cgcttagcgg aaacgtcgtc gcgaatgccg tgggacagca
     8581 gctacttgac caacagacag aaaccacgac acaaaaccag caagctcctg gcggtggaca
     8641 aatgccaggt aacagacaag aacaaggcgg tcaacgcgat ggtcaacaag gaaccacgaa
     8701 taatccattt gctgtttctg atcaagtcag cgaattggaa atcaatgtca aacctgcaca
     8761 attgggacta ttagccggag tagcatttgg aatcagtctg ttttctgtct ttttagcttc
     8821 tatagggata ttaagattaa acccaagaaa aatcctatta aactaaagga ggaatttgat
     8881 gttacggaca gaacaattgg gattttggta tcaagatccg caagatgcgc tttataaaga
     8941 tgtagaactg aatttcgaag ctggaaaaat gtacgctatt ttaggagcaa gtggttctgg
     9001 aaaaacgact tttctttcgc ttatttctgg actagatgca ccaaaagaag gaaaggtata
     9061 ctatcaagat cgttcactga acaaaatcgg gttaagaagt tatcgtaaaa atgatgtctc
     9121 gattattttc caagcttaca acttgcttcc ttatttatcg gctctagaca acgtcatagc
     9181 agcaatggaa atcactaaga ccaagcaaga gaattatcga caagttgctt tagatcgact
     9241 ggctcgtgta ggaattgatg aaatattagc aaaaaaacag gtgaccaaac tttcgggagg
     9301 tcagcagcag cgagtcgcta tcgttcgtgc tatctgctgt gaacatcaac taatcgtagc
     9361 ggatgagcct actggaaatc tagatgaaac aacttcacaa gatatcgtca aacttttcca
     9421 agaaatcgcc catgaacaaa atcgctgtat tatcatcgtg actcatgaga ttgaagtagc
     9481 aaaaagctgt gatgaagtct ttgaactaaa aaataaaaaa tttcaaaaaa tcgaagttta
     9541 aaaaacagga taatgatcaa ataaaaaatc cgaaccatat ctgtctcgca gatatggttc
     9601 ggatttttta tttgcttgat tcaattagta ataagtggtt tttatttttt ttcgtctttt
     9661 cgagaaaact tgtctttgac ttcgtcgcct aaatttcgag ctttttcttt gatgtcagca
     9721 cctagtgatt gtgctttccc ttctgtttct ttgctcttgt cgtctgttgc tttgccgtat
     9781 gcttcttttc cttcaccgac tactttatct ttcatatctt tagaacgtcc ttttaaatct
     9841 gccattgtgc ttcctcctct tagtttgttt attgatctac cttcagggta acatcaacaa
     9901 cagatacaga caaacagaac gctcgttttc taaaaaagat agttagagct gttgattaaa
     9961 aacttttttc gaaagtcccc catactttga cttctttttc ttggaccatt atttctcctt
    10021 tagcgataac tgtatcaata tcaagcgttt ctttatccaa gatacacaga tctgcatcag
    10081 caccaatttc gatttttcct tttttatcca atttcaatat acgtgctgga ttactggtta
    10141 tagctcgtaa ggcgatttcc aaagggatac tttctttttg cacagcttct ttgatcccta
    10201 ctagcaatgc cttggcactt cctactccta accctaggaa atgattgttt tcatcaaaat
    10261 aaggcaagct tccttgtcca tctgaggtca tcgtgaaatt atctaaagaa acatcttcct
    10321 cgatcaatcg tttcagtgct ttgctgaagc gaacttcacc atctgtcttc tcccaaaaat
    10381 caggatcttc gctagcagtg aagtcaatcg tgccaccacg tttggcaaat gccacgcaag
    10441 cttcaaataa ttctggtgtg cgatttgcat gagtaggcaa aaattgtgtg atcggaattt
    10501 ctgtttcttc tacgattcgt gtcaatagct ctagctttct ttttcctccg cctaagtgga
    10561 cattgatgat cccagctttt ccggataaca tgcctcctac acgagcatct gctactgcac
    10621 gagcgaactc ttcaaaactt ggctgagaag aacggtgatc ggatactgcg atttctccga
    10681 ttccgataat tttatcaatt gtcaaaaagt ctttgatgat agaatctgtt actgttttta
    10741 ccggcaatcg gtacgaacct tcgtatacat aagtggagat tccttctgct tccagacctt
    10801 tcgctttgct gatcaacgca gtcatatctc taccttctcc gtctgttccc aaacaaccga
    10861 caactgtcgt tacaccagct gtagtcaatt ggctcaatgt gacttctggc gttcgatttt
    10921 gataaccgcc ttctcctcct ccacctaaaa tatggaaatg acagtcaatg aaacccgggg
    10981 ttagtacttt tccctcacct gaaagttctt ctacttccac tccttcgtag ccgccttcta
    11041 gctgttcttc tacagccaaa atcttcccac cggcaatcag tacatccatt tttcctaagt
    11101 aatctggtgc aaacacttca atttgtcgaa tgattttcat acatttttcc tctttcttta
    11161 agtataactg atgataggat cttaaaaaat aatccaaacg ataggatctt tactcctttg
    11221 attttaccgt aaaatgacgg ttcttggagt ccttatctac tgtttggatt acttttttat
    11281 ttacaggtct ccagtactac tgcagaccta ttgcaatggc aatcatgatg aaaatagttc
    11341 ctaaaacaaa caggaatcct tgcatcttga tttggaatct tgcccatttt gcccattcga
    11401 tccgtgcaac acctagcaca ccaatcaagc tggctgatac gggtgtaaaa gcgtccataa
    11461 aacctgaacc aagctgataa gcaagaactg ctacttgtct ggatacacct actaaatctg
    11521 ccagtggagc catgattggc atagtcaaag ctgcttgtcc tgaatttgat gtaacaacca
    11581 agttgaacaa actttggaag atgtacatcg cccaagcacc aatgacagct ggaacaccgg
    11641 tcaacgccgt cccgatccca tgcaaaatcg tattcaaagc tgaaggaaca gatgcatcag
    11701 atcctcccaa gacaagaaga atccctttgg ccatacctac tacgattgct gtaccagcta
    11761 aatcagctgc accagattgg aaagaagaag cgatgtcatt gactttcatt ccatctaatt
    11821 tgaaaagaac agcaatgatt ccagcaacta atcccatcac aaagaattga gaagcgattt
    11881 ctggaatgta atagcctttt tgagtcacgc cccaaacgat ccagacaagg acaactaaca
    11941 tctctaataa gatcaattta tgcccaagca tgaatttttt attttcatca gatgtctttt
    12001 tgatatgaga acggaaataa tcatctgatt tgtaagtcaa cgaactttct ggttttttac
    12061 ggattttttc tgcatatatc atcatataac cagcggctaa tgcagtaaca actacccaca
    12121 taatcaatcg gaatgtcgca ccagacaaca cggggattcc agcaatccct tgagccaccg
    12181 ctacgctaaa cgggctcatc caagaggtcg catttccgac ttgagatgcg acataagtca
    12241 cggttactgc tacgatcgag tcatatccta acgcaatgac aaatggaacc atcaccattg
    12301 agaatggaat tacctcttct gccatcccga atgtagcgcc accgaatgaa aaagcaaaga
    12361 ataatagcgg taaagctaaa cgctccagtc ccttagtttt actgataaac gcatagatac
    12421 ctgcgtcgat cgctccggta cgcataataa taccaaaagc acctccaaca acaaggatca
    12481 atgcagcgat ccctacagca gatccatact tatctccaga tactagtcct tcaaaaacaa
    12541 aatttaagaa accaaagccg ccaaaatcat ctgttcccca tattttggct gttttatgca
    12601 attttttaga agtatcatag atattttcgc cgtactgatc gtaaagcaca tcatctgtca
    12661 aggagatttc atctagtttt tcctgcgtaa gatttttttc tccgtcagca atgacttttt
    12721 ccaatccttt ttccggaaca tccagttttt cacgttctgc tggatgatct tgcagttctt
    12781 ctaattgatc aaacacatac gacttatcca gctcgtaagc ataacggaag gaatcttgac
    12841 gtaaaacagt acgagtactt gtttcgccat ttgcatcttt gtattcaata tcttgcgtgc
    12901 tgaactttcc tgctggaacg acaaaagtca gtatccatgc aaagatgact acaccaaaaa
    12961 taatgacata cgtatggggt gtcgataatt tagtgaagtc gcgtttcggt tttcctgaag
    13021 aatttgtttt cttcaaattt atttcttcct ttcttttttc agtaattttg atgaataaaa
    13081 aatggataat caaatgaatt atacagtaaa aatgacattt ccacatgaat aatttattag
    13141 aaaatcactt ttttctattt tgtttttttt ctcctatgta tgattatttt tttctctttc
    13201 catttatatt aagtatttta tttctctgtt tttccttaaa attctttttt tatttttaga
    13261 ttagagaaaa tctcgtcctg tttttttcat tttacaatca ttgaaaaatt gaatttcatt
    13321 gcaaaaaaaa taataaacac ttataaatat ccattatcta tccttcaatt tttgtgtatt
    13381 ttatttctat tagaaaaagg tatcctgttg gacaattttg ttatatcagg taaacttcat
    13441 tgcaataatg ataacaaatg tactataatt actttgtaaa gagttttggg gaagactctt
    13501 tcatcaacgt gtattaagag acaagaaaga atccatatgg attctttctt gtctcttttt
    13561 ccactacttt ttatttttct gacactacat attcttataa ctcatttctc ataactgtga
    13621 aacaacttat aaaaataaca aactttaaac aaaacaagct taaaatcaaa gatgatccat
    13681 cttaaaataa ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata atcgttttct
    13741 ttttttcaaa cgctccattt ttatgtggaa cattattttt ttaaggaact ttgtcaacat
    13801 gtggataact attttccact tgtgtcctgg actagctgtt ttgctgtaaa atacaaaact
    13861 tgctgcagca aaaagatcgt tgcaagagag gctgttccgc agatcgctgg atctagattt
    13921 ggcaaagtca agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa atcagcaaat
    13981 tcaaataatc gcaaaccact gctatgttgc gatttgatac ttcgtgacaa atcaaatcca
    14041 gtaacaatga ttaatggatg accattttct ttgatccact gagctagttc gatgattgcc
    14101 gggtttcttc cttcattcga aagaagaaga aaaatgtctt ctgatttgac agaaagctga
    14161 tcaataattg cctgtcctgc accttctagc gtttcataga cacctccggc tggattttca
    14221 atcaatatac ttggtacaag atccggggtt tgatcccaaa attcaaatgc tacaccactt
    14281 agtgtgcggc tagcatatat ctgcaatcta ccaccttgat taatttgttt cgcgatccaa
    14341 gtgccagctt gaagcatcag cggctcttct atccttaatt tctcttccaa ttggtgataa
    14401 tagtcaaaat aacataaact catttatctc tcccgcctag ctatacttga catgaataga
    14461 aacagctgcc taatctaagg aagtacataa aaaaacagta gttagacagt aacacatgtg
    14521 gtctttgctg tttcaaacac aaaaaaggca ttgaacgaat acgcccaata ccttgacata
    14581 gcaaataaaa caacgtgcag accatggaaa atatctacat aggggtagaa ttttcaccct
    14641 agattaggta caataaatta ggtcgatgca ctgcattgac tatggcaaaa tgatcggtca
    14701 gggtgtatgt gtgtgactgg tcattccctg attcttagcg ccaactaagg gtcagggagc
    14761 ctttttttat tcagctgttt ctatctgaat ttattgtaag taatctctta tccaagtgca
    14821 agcaagaaaa tacaaaaaaa cattggtatg atttatatat atataaataa cataaaaaaa
    14881 ggaatggctt atcccgatac aacaagcatt cctttaaaaa agattctttt atataagtga
    14941 aaaaagcttt tttccaccaa ttaaataaat ctctcatctc aatagtttat ccattatttt
    15001 atcttttttt gcgtatacct actaaatatt accgtcttct tctcgttctt ctactttgtt
    15061 cgagagaatc tgtactagaa aaagtagttg tatgtctacc ttttctgcct atccgagact
    15121 ttcttttcat atatgggata taaatgatta gatacagtac agcaaaaaat aaagcaaata
    15181 cgcaagcttc ccaactacct aacgccaatt caaccatcaa ggtagcaacc ccaacggatg
    15241 ctgcatacca gataaaaatc agaaacgttt ctttttttct gaccatgcga aacatgataa
    15301 atagactaat aagcaatagt ggcaacacaa atatccaatt gcttccttcc atcagccact
    15361 tgagtatatc aagcagcgac tgtgcaacag cgactaattt tcccatagtg aacacacacc
    15421 tttttttata attaaaaaat tcgccttttt attctactat gaatcttctg gcttggcttt
    15481 catttctctt atgtaatcat tttcttttat acaacttaaa aaaaaattaa accattagca
    15541 taatattttc tgaatctatt cacgaaagtc tttttctatc tttcgttttc cacgactcaa
    15601 ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa gctgccgctt
    15661 taataacaaa tagcctttca ttctcagcag gccacttatt tccagttaac tttctgttct
    15721 tagcttttta ctcaactgga taatatatgc acttaatgaa gtcccaattt ccgaatgttg
    15781 aagtccatat tcaatatttg tcttcatata tcctaactta tcgcctacgt cgtatctttt
    15841 tcctttaaac tctctcgcat agaccgcctc tgtctggtta agagtctgaa gtgcatccgt
    15901 tagctgtatc tcgtttcctg ctcctggttc ttgagtttct aatatctcaa atattttagg
    15961 agtcagtaga taacgtccga taatggctaa attacttggt gcttttccca tttcaggttt
    16021 ttctacaaac ccttttactc tgcaacgacc cttttcgcta tctgttttag gatcaataat
    16081 tccgtattta gatgtttcat tttctggtac acgcatgacg gcaacagcgg aagcttgtgt
    16141 ttcttcataa tccatcatca attgttgagt caatgggata tcatctttca tcaaatcatc
    16201 ccccagcatg acgacaaacg gttcatttcc tataaatgct tttgcttgaa gcacggcatc
    16261 ccccagtcct cttggatgag attgacggat gaagtgaaga ttgacttgag tcgtttcttc
    16321 aactaaagcg agtaattctg ttttttcctt ttcccgcaaa ttgttttcca attcgatatt
    16381 tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc gttacgatca agatgtcttc
    16441 gattcccgaa tccaatgctt cttccacaat aaactggatc gttggtttat ccacgattgg
    16501 aagcatttct tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta gcccagcggc
    16561 aggaatgact gcttttttga ccttcactt
//
LOCUS       DACECR010000064        16197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.064, whole genome shotgun sequence.
ACCESSION   DACECR010000064 DACECR010000000
VERSION     DACECR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.064"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(21..905)
                     /gene="rsmA"
                     /locus_tag="IWO17_001890"
     CDS             complement(21..905)
                     /gene="rsmA"
                     /locus_tag="IWO17_001890"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ0290758.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            complement(898..1467)
                     /gene="rnmV"
                     /locus_tag="IWO17_001891"
     CDS             complement(898..1467)
                     /gene="rnmV"
                     /locus_tag="IWO17_001891"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ0290759.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            complement(1460..2236)
                     /locus_tag="IWO17_001892"
     CDS             complement(1460..2236)
                     /locus_tag="IWO17_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0290760.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            complement(2391..3713)
                     /locus_tag="IWO17_001893"
     CDS             complement(2391..3713)
                     /locus_tag="IWO17_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0290761.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            3888..4475
                     /locus_tag="IWO17_001894"
     CDS             3888..4475
                     /locus_tag="IWO17_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290762.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            complement(4954..6963)
                     /gene="metG"
                     /locus_tag="IWO17_001895"
     CDS             complement(4954..6963)
                     /gene="metG"
                     /locus_tag="IWO17_001895"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ0290763.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            7231..7509
                     /locus_tag="IWO17_001896"
     CDS             7231..7509
                     /locus_tag="IWO17_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290764.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            7606..8427
                     /locus_tag="IWO17_001897"
     CDS             7606..8427
                     /locus_tag="IWO17_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0290765.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8712..9722)
                     /locus_tag="IWO17_001898"
     CDS             complement(8712..9722)
                     /locus_tag="IWO17_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0290766.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(9734..10687)
                     /locus_tag="IWO17_001899"
     CDS             complement(9734..10687)
                     /locus_tag="IWO17_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0290767.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(10677..11747)
                     /locus_tag="IWO17_001900"
     CDS             complement(10677..11747)
                     /locus_tag="IWO17_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0290768.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(11753..12796)
                     /locus_tag="IWO17_001901"
     CDS             complement(11753..12796)
                     /locus_tag="IWO17_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0290769.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(12797..13738)
                     /locus_tag="IWO17_001902"
     CDS             complement(12797..13738)
                     /locus_tag="IWO17_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0290770.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            13822..14169
                     /locus_tag="IWO17_001903"
     CDS             13822..14169
                     /locus_tag="IWO17_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ0290771.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(14239..15900)
                     /locus_tag="IWO17_001904"
     CDS             complement(14239..15900)
                     /locus_tag="IWO17_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0290772.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         4580 a         3329 c         2897 g         5391 t
ORIGIN      
        1 ttctgtcaca acctcttttt ttactgcata ttttctgaca tcgcattgct taatgcggcg
       61 aattcttgta atgacaaagt ctccccacga cgttttggat cgattccagc tgtttccaag
      121 ctttttgcta accatgcttt tgtctggtca tctttgccat acgaatgctg caagttattc
      181 caaagcgttt ttcttctttg ctgaaatgcc gcttttgtca atcggaaaaa tgctttttcg
      241 tctgtcactt cgactgccgg tatatctctt cttgtcagtt tcaaaatcgc tgaatctaca
      301 tttggctgag ggacaaatac tgtttttggt acgatgaatg ctaaactggc ttccatataa
      361 tattgaacag caatcgataa tgaaccataa gctttcgttc ctggttcagc agagatacga
      421 tcagctactt ctttttgcat catgacgact agttcatcca caaccaaatc tgattcaaga
      481 aaatgcatca tgatcggtgt cgtaatatag taaggtaaat tcgctacgac ttttaacgga
      541 agctcctcat gaaatgtttc tctaacagct gttgacaaat cggtctttaa aatatcttga
      601 tgtacaatag tcacattgtc atagggttgc atcgtatcag ccaatacagg gatcaaacga
      661 tcatcgatct caaatgctac gacttgtttt gcatgcttgg ctaattgttc cgtcaatgca
      721 ccgattcctg gtccaacttc gatcacgttc gtatgttcat ctatttgcgc agtttctacg
      781 attttcctta aaatatttgg ttcagtcaga aaattttgtc ctaaactttt tttaaatgaa
      841 aaaccatgtt tcaataaaat ctcttttgtt ctagagggtg tcgcaatttc acgatattca
      901 ctcatgttgt tcctccacta ttttcattgc ttgggcgagt tctttcggcg taatacgaaa
      961 catcttcaaa cgtttttcca attgtttgcc gttcgtgtaa ccgatgcgca actcgtcacc
     1021 tagtttttct cgtttttctt ttgcttgtgc cccagctatt aaaccatagt ctaaaaggtc
     1081 ttgtctagtg atttcagaaa cagccgtttg ggaaatgtct ggcgtcacga cctttttcaa
     1141 tgcttctaaa atagcagcat cacttgcatg ctcgacacca aggcttccct ttttctttgg
     1201 tttagcttgt gtgcgcggca aaaaagcatg ttttgcctga ggaacagctt ccataatgat
     1261 ttttcttatt ttttctcctg aaaaatcagg atcggtaaaa acgatcactc cacgagtctc
     1321 ttgggcatgt tcaatttgca tcaggatctc ttcattgatt gcagacccta ttgtttctat
     1381 cgtatctgca tcaactactt cccgcagcct tcttgtatcg tcttttcctt caacgactat
     1441 aatctcttgg atcttcatct tatccatgtt cgcttagacc aaataatcgg tgcgcatttg
     1501 tccatgtttg ggcagccact tcttctgtcg gaagattccg aagttcagca atcttttcta
     1561 ctgtataacg agtatagcct ggttcatttc gctttcctct aaaaggaact ggtgccagat
     1621 aaggggcatc tgtttccaca agtaatcgtt cgattggtac atttgctgca gcctcttgga
     1681 cgtctaatgc ttttttgaaa gtgacgacac cgctaaatga aatatgcatc cctaagtcaa
     1741 gaaatcgttt tgcccattca taatcaccac tgaaactatg catgattcca ccgatatctc
     1801 gaacatcttc ttcttttagg atttgatacg tgtcttcaag tgcttctcta gtatgaatac
     1861 tgattggtag gttcatttct tttgcgatag caatctgtct gcgaaatact ttatcttgga
     1921 cttctttcgg gtcttccatc caatgataat ccaagccaat ttcccctaat gctacaacct
     1981 ttggtaaagt caattgttct tgtaatttat tttcgatttc tttcgtataa ctgccagcct
     2041 ctgtcggatg ccagccgata atactgtata tatttggata gtcatggctt aattgcaaag
     2101 acttttcaat cgttggcgta tcgaagccca cgaccgccat tttcgttacc cccatttctt
     2161 gtgctcgctg aatcgtttct ggaatatctt cattgaactg ttcagcattt aaatgggtat
     2221 gagaatcaaa tatcatgttt atctctcctt ttcattctta ctttagcaca taagcactac
     2281 ttgcagaagt agcaaaaatc aaaatagaag aacttgtttt attgaaatag aaaaagcacg
     2341 aagatctaga ggacctagtt cgtacttttt ctgtctgttg ttgaaatatt ttactcttct
     2401 gagcgttttt tctttttttg ataatagact gggataccta tcaaggtaac acctataccg
     2461 atcaatgcta atttcgtttg cgtcatcatc gtcataccta aaatgaaaat agcaccgaga
     2521 atcgcaataa tcggaacgat tggataaagt ggtactttat atggacgtgg caattccggc
     2581 gctttttttc ttaaaataaa gacaccgata aaaatcagta aactgaatag ccacatcaca
     2641 aaaatcaaca tgtctgttaa aaagtcgaaa gaaccaaggc tcatcataat acaagctact
     2701 gctaactgga aaaccgcagg aacataagga accgtaaact tctttgaaag ctttgtcagc
     2761 tgtttactga aaggtagctc gtcttctaaa gccatcgcat aaggcacgcg gatcccagtt
     2821 agtgtataac cgttcaatgc accgtatacc gagatcaaga tcccaatagt gaccagcttt
     2881 ccaccgattc ctccaaaaat gacgtctgat gcttcagaag ctgcattcaa attaccggca
     2941 agatgatcaa tcggcaaagt tttcaaaaaa acaaaattga tcaatagata aacgactgtt
     3001 acaaaactca aaccaagaat aattgcttta ggcaaatctt tttccggccg tttcatttcc
     3061 cctgccattg caccgacacc taaccagccg tcataagcaa acaacgttgc taacaacgca
     3121 ctgctcaatc cttcgacaaa agtaacatct tttccagctt cgattggaaa taactggaca
     3181 gttccttgtc ctggggtcaa taatccccat atgacaatca cagcaattgg tattaattta
     3241 acaatcaacg tcactgattg tacagaagca ccgacttttg tccctaacaa attcaaccct
     3301 gtcacagaaa cagcggtgat cactgcaatc aacaaaagat aatcggtaga taattgtaat
     3361 aagttcgtca attgtgtcgc aaagataatc gataatgcag caatattagc aggaaagtaa
     3421 atcaagcttt gcgcccatcc taataaaaag ctgggcaatt ttccatacgc agcttcaata
     3481 tattttacag ctccaccagt ttccgggatt gctgtagcta attcagccac tgtcaatcca
     3541 gcacagatcg ttaagaaacc acccaaaagc caagctagca atgtcaaact tgtagactga
     3601 gtactagcag taacagcagc agttttgaag aagacgcctg caccgatcac ggttcccata
     3661 acggtagcta atgcgccaaa cagattgatt tctcgtttta gtttgccttt cattcccatc
     3721 ctctttcttc tttgaaattt ggtataattt tcagtataac tgagaatatt ttaatttacc
     3781 attctttttt ctccactttc agtgaaaaac tccctcttaa gacggatgta tttctttttt
     3841 atttttcgta tactaaacct accttttcca cgaaggagga acagtttatg gattttaagc
     3901 gttcttatcc gaaattagca gattatttat ccaaccgggt cgatttttcc cgtaaagaac
     3961 aacagcagtc ccattggcat tggttcacat taattttatt tggattaatg actgttgcaa
     4021 cgttatcaca aaaactaata ttgctgactt tgttcatcgc aatcgttgca ttgatcaaag
     4081 gtcctgtgat gatcttgtgg gggatgttgt attccttttt ggttgggctt tttccgccta
     4141 tcggtatttt gctttcagcg attttctttt tattgaatat cggaacattt acaaagaact
     4201 ggcggatgac cttagtcggg atctattttt atttctatcc ttttggagtg atggcactta
     4261 gtgaagtcat gcattgggat aatcattggt ttatagctgg ctccttgctt ttagggttga
     4321 tcttgttaca cgtgatgttg acaaagctct atcaacatta tggtatcgga cgtacgattt
     4381 tctggtatgt cttttcgatc ccatttgcac tgcttactgc tctcttacca agccgtttga
     4441 agacaaaaat aaaaggttac cataaaatca agtgatagga tttttacaaa ataaagaaaa
     4501 caatcatcga agtcaaagcc gatgattgtt tttttatcta tattttcaat cacacatata
     4561 agagcacaaa ctaattgaac ataaagtgat agaacttata attaacacat caacgctact
     4621 gttagatata aatatagatg taataaagtc attgcattca aactttatga atgtaaggac
     4681 aataaaaaag agtaccagat gaatcaatta aaatctgccc gaccctttat cattatgctt
     4741 tttttctgtt tttacgattc ctattagtat tattctaaat taacaaaaat cagcgaagct
     4801 cgtacttctt tttctaggat ttattttgga tagttctctg tttctgtatt atatgattgg
     4861 cttcaagtaa taagaaggaa tttacgaaaa ttgcttctca aatttcggaa aaagggggta
     4921 tgacacaact agtgtcatac cccctttgaa tttctatgcg atttctgagc catttggcat
     4981 ggattttggt gcttcgatta cttgtaaaga gccatctggt gcttctgcag aaaggatcat
     5041 tccttgactg atttgtccac gcatctttcg tggtttcaga tttgctacaa tgacgacttt
     5101 tttcccgatc aattcgcttg gatcaggata aaattcagcg attcctgaca agatttgacg
     5161 gtcttggctg tctccagcat ctagtcggaa ttgcagcaac ttatctgccc cttctacttt
     5221 ttgacagttg atgacttctg ctacttttag ttctactttt tcaaatacat caaatttgat
     5281 ttgtttttct ttcgttgaaa caagttctgt ttcttctgga tcccatttga ctgtatcttc
     5341 gtttgtttga gtaccttcag acattttttc ttgaataaaa gcaacttctt cttccatatc
     5401 taaacgtggg aagattggtg taccttttgc aactactttt gttcctgaag gaaattcgcc
     5461 aaagtgtaat ccttcaagat tcattgtttc agagtccaat cctagttgat tgaagatctt
     5521 ctctggcgtt tctgtcatga ttggctgaag taatatcgct gcgatacgca aagattcagc
     5581 taaatggatc atgacactat tcaattcagc tttcttttct tcgtcttttg ctaataccca
     5641 tggttctgtt tcatcaatgt atttgtttgc acgagaaatc agcacccaga tttcagccaa
     5701 tgctgtattg aattccattt tttccattgc atcatggtaa cgtccgacaa cgttagcagc
     5761 cgtcgtagac agttcactat caaatggagt gacaagtgag gcatagtcag gaacgagtcc
     5821 atcacaatat ttgttgatca tagcaatcgt tcgattcaat aagttcccta gatcatttgc
     5881 cagatcataa ttgacacggg aaacaaaatc ttctggcgta aagacaccat ctgacccaaa
     5941 cggaacagca cgaagcaaat aataacggag tgcatctaaa ccataacgtt ccacaagcat
     6001 ttcaggataa acgacatttc cttttgattt agacattttt ccgtctttca tcaatagcca
     6061 gccatgccca aatacttttt taggtagagg caaatcaagg gccatcagca tgattggcca
     6121 atagatcgta tgaaagcgaa cgatttcttt accgaccatc tgaacatctg caggccagta
     6181 tttttcgaat agactttcat cttcggagcc atatcctagt gcagtaatat aattggataa
     6241 tgcatcgatc catacataca ccacatgttt cggatcactc ttgactggta ctccccaagt
     6301 aaaagttgtt cgtgaaactg caagatcctc taatcctggt ttgataaagt tgttgatcat
     6361 ttcgttttta cgagattctg gctggatgaa ttcgggatgt tcttcatagt attccaatag
     6421 acggtctgcg tatttgctca tgcggaagaa gtatgattct tcttttacca attctacttc
     6481 gtgtccactt ggcgctttac caccgatcac gttaccttcg tcatctttgt acacttctgc
     6541 aagttgtgtt tctgtaaaga attcttcgtc agaaacagag taccagcctt catattcacc
     6601 taagtaaata tctccctgtt ctaataaacg atcaaatatt ttttgaacag ctgctttatg
     6661 gtaatcatct gtcgtgcgaa tgaatttatc ataactgata tcaagcgttt tccataattt
     6721 cttgacatca gccgccattt tatctacata ttcttgcggt tttacaccta gttcttctgc
     6781 ttttgtttcg attttttgac catgttcatc cactccagtg agataaaaca catcaaagcc
     6841 catcaaccgt ttataacgag caattgcgtc acatgcaatc gtagtatacg agttgccgat
     6901 atgaagtttg ccgcttggat aatagattgg cgtagtgata taaaaagttt ccttttctgc
     6961 cacggggctc ttcctccttt atcgcatgat gcgaattcgt ttccttgcta gtataccata
     7021 aaaaaagcat gctgttagct ttttttcaaa atgaaaaaga ctggataaac gacttctatc
     7081 tacgttcaat ctatccttta aaaggatgaa taggttgaat aaatgcgggt ttttgttttg
     7141 tttcatatct actaaaaaac ggctttttat ttctcccctc acacgtggta taataaagac
     7201 tatctatctt agtaagggac tggaacaatc atgcagaaga aacatctttt ttttacactt
     7261 agtattgctt ttctttcatt agcacattta atattcagct atttttatat tcgtatgtat
     7321 ggttatttta acctccatgg gcatttgaac agttttatga cagcagcttg gattttgcgt
     7381 tttatcattg acgtatatat cgttatttgt ggcttctttg ccattcgtga agagcggtat
     7441 aaagtattgc cgttttatct gcttttcttt ttattcaatc tcatacttcc atttattttt
     7501 catatttaaa tgataaccta gatttttcaa gtaaatcctt ggtttttttt cagagtctgc
     7561 tacaataagg taaaaagtac ttttactgtc aatggaggtg gtcagatgaa ccgttttgct
     7621 tctaaagaag aagaaaaatt atattatgaa cataaagcaa gtgaagaaga atttcttaac
     7681 tggtatcaaa aacaggaacg tccagaatat gacaagccct ctttgactgt cgatatcgta
     7741 ctgatgtgct ataacaaaga agaagatcag ctgaaagttt tgttgatcca aagaaaaggg
     7801 catccttatc gaaattcatg ggcattgcct ggtggctttg tccaaaagga tgagtcaact
     7861 ggagaaagcg tcttaagaga aacaaaggaa gaaactggtg tggttatttc aaaagaaaat
     7921 attgagcaac tgcatacatt cagtacgcct aatcgagatc cgcgaggctg ggtcgtcaca
     7981 gtcagctact tagcctttat tggagaagaa ccgctaatcg ccggagatga tgccaaggaa
     8041 gtacgctggt tcacactaga acgtcatagt aacaagatcc atttatctag cggagaagtg
     8101 gctatctcgc ttgatttagt cactggagag tcttctggca aagatacttt ggcttttgac
     8161 catagtcaaa tcatcttgaa agcattcaac cgtgtagtca ataaaatgga acacgagccg
     8221 caagttctac aagttttagg taaagatttc acgattacgg aagctcgaaa agtatttgct
     8281 aaattcttag ggatcgatta taaaacgatc gatcattcca attttaagaa agctatgctc
     8341 caatatttcg atgagatcgg tgaacgtcct gtaggaatcg gccgtccttc aaaaatctat
     8401 cagctcaagc cggggcacca tgaataaggt tgtgagcaaa agattcagac ctaatcattc
     8461 aagaaaaaac gcaaaaacaa ctctttgtga gttacgtttt ttgtttcaga attttgatgt
     8521 attcgtaaac tttctgaata aaaaattaac aatagcagaa aaagctcgta cgaacttttt
     8581 ctgctattgt tttattggtt tgtcataatt agtgagcttt tcttcaaaag aaaaattcct
     8641 agaaacaaaa gtcattaaga ctttttatta acaaggctaa ccctaacgct cctatcagtt
     8701 tcattcattt tttatccctt gataaccgaa tgtctgccca ttttttctac tacttggctc
     8761 tttcttacgg aattgctttc ttctgctccg tcggtattga tcaaaagaaa ttttttatta
     8821 taccattggg acttataacg caattttgtt attaatttat gccagtcgtg aatagaaaaa
     8881 tcagcatgtg cctcttcttt tggaaaagat tctacaaata agtagtcata ttgtttgacc
     8941 aatttcccag ataattgttc aagataatct tctcgctggt tcttttgttt cagataaaga
     9001 tccattactt ttctcttttg tttctgataa ttgcttgcct gatctaacct gatctttcta
     9061 tggtgagcgg agcggacttt cacattcaat ttccgctgtg catgttgcag cttttcttct
     9121 gtctggtgaa attttggtaa agttacttct agaggtcgag atgtttggat caatgtcttc
     9181 ggatgatagg cgatcccgat ccatttagct aaagattctt tttttactgg cgaaacatca
     9241 caaagtagtg cgacataaaa ttcttcatta tatcgagcag aaatcgtagc ggagcgaatc
     9301 gatccttcaa ctggccgatg ctgatttatc ttgattagtt ctttttgttt tggtaacttt
     9361 aagtagccat tttccagata gatcgtacgg gtttggttgt tggtcgtata agattgccac
     9421 atactttttt tcgttttaag ctttgggaag ctggcacgtc ccttgaaata attgcgaaaa
     9481 gcccgatcca aattaagctg tgcatttgct aaagcaagac tgtctacttc ttttaagaat
     9541 gggtattctt tttttaatgt agcgggtgtg agcgaaaaat tggctactcc tgtttgttgc
     9601 caacttttct gccgcttttt cagcaaatgg ttataggtaa atcgagcaca tccaaaagta
     9661 cgaatcaatt gctgtttttg ttcttctgtc ggatacatac ggaatttaaa agcttttagc
     9721 atctatgtcc ctcctagtta gcaaccagaa gagattcctt ttgtcgtttt agtttttttg
     9781 cgtaatatgg gacttcctgc tcagttgcat agacaaaatg ttctggtgcg atttcacgaa
     9841 gttgacgtac ttgtccgtca tctggttcag gttgatattt gattcttctg cgttttctct
     9901 catggtctgg atctggtaga ggaatagcag ataacaaact ttctgtataa ggatgaacac
     9961 cgtgattata gatttcatca cttgttccca tctctaataa caagccgtta tgcattacac
    10021 cgattcggtc actaatatgt ttaaccatcg acaaatcatg tgcaataaat agataggtca
    10081 attgatgttc tttttggaga tcttgcaaca aattgaccac ttgggcttgg atcgacacat
    10141 ctagtgcaga aattggttca tcacaaatga taaaacgcgg ttttactgcc aatgctcgtg
    10201 caataccaat acgctggcgt tgcccacctg aaaattcatg aggataacga gtaccgtggc
    10261 ttggattcaa tccaacagtt ctcaatagat catcgacttt ttttgcacgc tcttcagatg
    10321 atttagctag tccattaaca tcaattcctt cggcaatgat atctctgact ttcatacgag
    10381 gattcaacga agcgtatgga tcttgaaaga tcatctgaac atcatgccgg aatttcgtca
    10441 aacctttttt atctttgatc ttcgttatat cttgaccgtc gaataatatc tcaccatcag
    10501 taggttcatt caagcgaatg atcgtacggc cagtagtgga ttttccgctt ccagattctc
    10561 caactaaacc gaatgtttct ccttcataaa tgtgaaagct gatgtcattt acagcatgta
    10621 cttcattctt ttttcctaca ttgaaatact gtttcaatcc tttaacttca agtagtttat
    10681 ttggcatcag caattcctcc ttgaccgtaa gtatgttttt gtttctcttt aaagatagcc
    10741 cagcgacgtt ggatttcttc tggcggtgtc actttgggtg cctgcggagc aagtagccaa
    10801 gttgctgcct tgtgtgtttt tgatagttca aaaaatggtg gttcttgttc tgcatcgatt
    10861 ttcatcgcga attcatttct tggatagaat gcgtctcctt ttggcgggtt caataaatcc
    10921 ggtggagaac caggaatagc atacaatcga tcgttcgcac tatctaatgt cggcattgaa
    10981 ccaagcagtc cccaagtata gggatgttgc gggttgtaga aaatttcttc cgatgttcca
    11041 acttcaacga ttcttcctcc atacatgaca gcgacacgat cagcgacatt cgctactaca
    11101 ccaaggtcat gagtgataaa gataattgat gtatcgattt ttgtctgaat ttctttcagc
    11161 agctccaaaa tctgtgcttg aatagtcaca tcaagcgctg ttgtaggttc atcagcaatc
    11221 aggatttccg gataacatac cagtgcaatc gcgatcacga tccgttgacg ctgacctcct
    11281 gagaactgat gcggataact gttgatacgt ttctcagctt cgggtattcc aactaattta
    11341 agcaattcta atgcagcttt ttgtccctct ttttttgaaa ctttattatg ctttctaagg
    11401 gactcagcga cttgcttccc aatgggcatc gtcggatcta aagaggtcat tggatcttgg
    11461 aaaatcatcg ctatttcttt accacggatt ttttgcatct ctctttctga ttttccaacg
    11521 atatcgttac ctttaaagag gatttgacca gcatctatat tggcgttgct tgacagcaag
    11581 cgcatgatgc ttctggtcgt cactgatttt ccacttccag attccccaac gatcgccaat
    11641 gtttctcctt tattcaaatg aaaattgaca ccgcgaatcg ctcgtacctt tccagcatac
    11701 gtatcaaaag agatttctaa gtctttcact tctaaaattt tcgtcatcgt cttcactctt
    11761 tcattttagg atcaaaggca tcacgcaatc catcagcaag taagttaaag ccaatcatga
    11821 taaccgataa agttactgcc ggaatccata atagataagg cagatacagg aatgtcttgt
    11881 acccttcgct caacatcgtt cctaatgaag ctgacggagg cgtaagacct aaaccgataa
    11941 agcttaaaaa ggcttcgaag aaaatagcag atggaatact aaacatcatt tgaataataa
    12001 tgacactaga gatattcggc aaaatatgtt tgaatgcaat tttcattctt gattcaccta
    12061 atgtcaatcc agctaaaaca tattcttgat cttttaattt cattgtttgt gcacgaacga
    12121 tacgtgccat tggtatccag tttgtgatgg ccatagcagc cacgattgaa aaaattcctg
    12181 gttcaaaaac gaccaacatc aaaatcatga caacgaggtt tggaattcct gataagattt
    12241 ccaaaaaacg ttgcatcacg ttatcgacac gtccgcctaa caaaccagaa accaacccgt
    12301 aagctacacc aatcgttaca tctagtaatg cagcaataaa tgcgatcagc aacgagattc
    12361 tcattcccat aaataagcgg cttaatacat cacgtcccaa accgtctgtt cctaaaaaga
    12421 aatttacgtt tgaagggacg tttgcttgag catatttatc aacaagttca ccaccaacca
    12481 ttgcttttcc attcaatcca tcgatattga ttcccggaat acgaggaggc aaattgatat
    12541 aagccacgtt ttgggcagtt ggatcatgag gagaaacaaa gattgtgatc acagagatca
    12601 aaatgatgac caatagtaaa accattgaaa taacggcagc tttgtttttc tttaaacggc
    12661 gccatgaatc ttggataaaa ttcaaagaag gtgcagtaat ctgttcacgt tcttcttcgg
    12721 tatttttttg taatggaaga aattcatccg cagggatatc tgcaactgtt tcataacgct
    12781 ttggtatcat ttccatttat cctcggctcc cttctgaaac acgaattctt ggatcaacta
    12841 gaccgtacaa tagatccacg atcaaaataa caaaaatcag cataaatgag taaaggatcg
    12901 ttacagccat gatcgtagga taatcatttg ttgtgattga cttaacgaat tgttccccga
    12961 taccaggaat agcaaagata ttttctacca ccagtgatcc ggtcatcaat gctacagcta
    13021 acggtcctaa taaagtcatc aatggaatca aactatttcg aagaccatgt ttaaaggcga
    13081 tttcccaacg gctcaatcct tttgcttttg ctaattccac ataatcacta tgcagtactt
    13141 ccaccatttc cgtacggata aaccgagcag agtcagcaag cggcgacatg gccaacgcaa
    13201 ttgttggtaa gattgtgtac acaaagccat cccattttgc gattggcaaa acttgtaatt
    13261 tgattgcaaa gatatattgt aataaaacag caaatacaaa gtttggaatg gaacgaccta
    13321 atatcgctac gagtgtagaa gatgtatctg cccatgtatt ttgcttcatg gcagagatcg
    13381 ttcctaaaat cgtccctagg actgttccaa agataattgc ctgtaaccct aactgcaagg
    13441 atggtccgat tcttccagct aacaaatgtg cgactggctg gtttttaaat tggaaggaaa
    13501 ttccaaaatc tccttgaagc aaattagaaa gataaatccc atattgtacg ataactggtt
    13561 tatccaaacc gacttgttta tttagcatct cgattgtttc aggactcaaa cgttcctgat
    13621 tcgtatatgg cgtgcccggc agcaattgca tcaagaaaaa ggtgattgtt gcaattaacc
    13681 aaagcgtgat gatcatgaaa aagacacgtt ttaatacata tttgatataa ctattcacta
    13741 atgtttcccc ctagcttttg acttctgaca gatgtttcgc tacattatta gataatgaac
    13801 caaaaataaa ggagcaatca aatgaaaaaa aaatctatcc cctacgcagt agcttttcta
    13861 ctgattctgg ttattttaat aaaaaatctc atcaaccatt cctttacact cattcaatta
    13921 tctaatgatc tattcttatg gtcattgccg tttctgatta tcggcggttt tttatgggtc
    13981 ttttcttcgg gtttcttcga tcattttcag cgatcggtcc atcttgcacg aacaaggaac
    14041 cgaaagaaaa aaccagaatt ttcctcgctc tcgtctgcca gctacggtat gtacagcttt
    14101 tggctgatca ttgccggaat actaattgct ctttctgcta ttttcatgct tttttctttg
    14161 ttaggataag tgaatatgag aacgaggttg cgacatttgc cgcagcctcg ttttttctca
    14221 tgctcacggt attaacgatt attcttcaac gtatgtccat ttgtaatccc atgaagcgcc
    14281 tgccgcatgg tgaacgatac ctttgatttt ttcattcact aggtgacctt ctgctttttg
    14341 gtacaccgga acgacaccca tatcatcaga gatgatcttg attgcttttt ggaaatcatc
    14401 ccaacgggct tgttcatcgc cagcgtctgt tgatgaagct gctttaactg cttcgtcata
    14461 ttctgcattg ctccagcgac cacggttgta tgaattacct gtgacaaaca aatcagtaaa
    14521 gctgcttggg tctgcatagt cagcagccca tccgcctagt actgcgtcaa aatcacccga
    14581 agttgagcgg tcaagacgta cagagaatgg aacaggtgtt ggtttgactg ttacgccatc
    14641 caagttttct tggattgagt tttgaacata ttcgattact tttttcgatg aatcatcatc
    14701 agaagccatc aaatcaaatt ctaaagaatc aacgcccaat tcttttttcg ctttttccca
    14761 atattctttt gctttatctt tgtcataaga aactaatttt ccagcttctt ttgcaaagtc
    14821 ttcgttcgtt gttggacttt ttaccatatc tgatgggatc aatcctgtag agacaactga
    14881 tccgtcacct agtacttgtt ttactaaagc ttcacgatcg atagaataag aaatagcttt
    14941 tcttaggttc acattgttga atggtgaatt cttttcacgt tgattgaatt cgatatagct
    15001 tgtccgcgct tctttaacag aagtataagc tggatcgttt gcgttttgct gtgctaactc
    15061 accagacaag atcacgtcat ctgcttggcc atctttgaat aagttcaatg cagtagaaga
    15121 ttcttttact acactgactt tgatttcaga aagttttact gtatctttgt cccagtaatg
    15181 atcatttttc ttataagtcc attctgtatc tgttcctggt ccatcaaaat cttctaatgt
    15241 gaatggtcca ttataaacag ctttatcact tgcagatgca taggcatccc cattttcttc
    15301 cactacggat tgtttttgtg ggaaaaatga tgggaaagca agcagatatt ggaaatatgg
    15361 tgtttgtttc gtcaatttga tttctaattc atagtcacca actgctttga tccctagttc
    15421 tgatttatct ttctttccag ctgtaatgtc ttctgcattt tcaactggcg catacaggta
    15481 agcatattca gaagctgtag ctgggtctac tgttcgttgc cagccataaa cgtaatccgc
    15541 tgctgtaaca ggatcaccat ttgaccattt tgcgtcttct cgtaatttga ttttgtaagt
    15601 taatccgtct tcactgactt ctgccaactc gctagctcct gccggctgtg gtttgctgtc
    15661 ttcatcaaga cggtaaattc cttcataaac gttattcaat gctgtaaaac tgattgtatc
    15721 tgttgcaact gataagtcag ctgtaggcat ttcttgaggc acgactaagt taaatacgcc
    15781 tcctccatct gctgaaccgc tttttgctga actactgctt gtggctgaac tgccgccata
    15841 acatccagca agtacaaggc cgcataccgc aaccaaacca aatgtaaatt tctttttcat
    15901 tttacgtacc cctttattgg aattttcaga ataattaata ttttctgaag ttgtttaaga
    15961 gtttaatgaa taattattaa aaaatcaaca cttttttttg tctttttcat aaaataatga
    16021 ttttttcgtg aggtttcaag gaatttttca gaaaaaatag ctttgtacaa ggttttccat
    16081 ggtcttccaa aagccattaa ataattttcg ttatattgtc tattattgcc atacaaagat
    16141 aaaaaagaat aaacaaaaag aaactgtgac aaaagtcttg tcacagtccc ttatctg
//
LOCUS       DACECR010000065        16143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.065, whole genome shotgun sequence.
ACCESSION   DACECR010000065 DACECR010000000
VERSION     DACECR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.065"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(182..601)
                     /locus_tag="IWO17_001905"
     CDS             complement(182..601)
                     /locus_tag="IWO17_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ0290773.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(603..1187)
                     /locus_tag="IWO17_001906"
     CDS             complement(603..1187)
                     /locus_tag="IWO17_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ0290774.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1234..2709)
                     /locus_tag="IWO17_001907"
     CDS             complement(1234..2709)
                     /locus_tag="IWO17_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ0290775.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            2890..3201
                     /locus_tag="IWO17_001908"
     CDS             2890..3201
                     /locus_tag="IWO17_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290776.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3316..5652)
                     /locus_tag="IWO17_001909"
     CDS             complement(3316..5652)
                     /locus_tag="IWO17_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0290777.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            5794..6462
                     /locus_tag="IWO17_001910"
     CDS             5794..6462
                     /locus_tag="IWO17_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0290778.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6465..7508
                     /locus_tag="IWO17_001911"
     CDS             6465..7508
                     /locus_tag="IWO17_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0290779.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(7745..8626)
                     /locus_tag="IWO17_001912"
     CDS             complement(7745..8626)
                     /locus_tag="IWO17_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0290780.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            8785..9513
                     /locus_tag="IWO17_001913"
     CDS             8785..9513
                     /locus_tag="IWO17_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ0290781.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            9669..9986
                     /locus_tag="IWO17_001914"
     CDS             9669..9986
                     /locus_tag="IWO17_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0290782.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            9979..10587
                     /locus_tag="IWO17_001915"
     CDS             9979..10587
                     /locus_tag="IWO17_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ0290783.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            10603..11433
                     /locus_tag="IWO17_001916"
     CDS             10603..11433
                     /locus_tag="IWO17_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ0290784.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            11571..12059
                     /locus_tag="IWO17_001917"
     CDS             11571..12059
                     /locus_tag="IWO17_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0290785.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12110..12982
                     /locus_tag="IWO17_001918"
     CDS             12110..12982
                     /locus_tag="IWO17_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ0290786.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13158..13520
                     /locus_tag="IWO17_001919"
     CDS             13158..13520
                     /locus_tag="IWO17_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0290787.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            13529..14281
                     /locus_tag="IWO17_001920"
     CDS             13529..14281
                     /locus_tag="IWO17_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0290788.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14295..16097
                     /locus_tag="IWO17_001921"
     CDS             14295..16097
                     /locus_tag="IWO17_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0290789.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
BASE COUNT         5025 a         2811 c         3306 g         5001 t
ORIGIN      
        1 ccatgtttac aaaatttttc ttactactcg aaacgaagcg gctttatcac aacctcttga
       61 taaacggcgt tcaatcagct gtccctcggt attagctcaa aaacggcaaa atatgagaag
      121 ctatttttcc atttttttct ctcatactca ggtcataacg ctgatttcac aacctcttct
      181 ttcataatgg tttttcacca tctttataat aaactggttc tttgatatgg aaatctattt
      241 tttccaacca atctaaaatc ttgtaaccta actcttctga catcggaaaa gaggcgttga
      301 tttgtgcatt ggagatatgg tcaacatgga taccggaagt gatcgtacaa cttcccggaa
      361 cggcagtttt gatgatcaca gctatttttt cggctaagac atcgtcttta tggaaccgac
      421 catcgtggct agggaaacgt acagtttgca tctctgtatc acgagaaaat gtagtgactg
      481 ttccgatatg tggtgtatct ccgcctgtta aagtgatcaa taagtcttta ccgattactt
      541 taacagcagc ttggatcgtg aaacctagtt tggttacttc aactatcgtt tctttttcca
      601 tattaatcac cctgccaacg ttttgaatgc tcatttacaa tttcaaattg atccaaaagt
      661 ttcattgtat ggtgattaac aagatcttgg atcgtttgag gctgattata aaaagcagga
      721 attggtggta tgatttgcac acctagtcgt gctaatttag tcaagttttc caagtgaatg
      781 gcatttaatg gcgtttcacg aggaacaatg actaactttc gttgctcctt cattgttaca
      841 tcagcagcac gtgcaatcag gttgtcgcct aaaccaacag agatacctgc gactgttttc
      901 atacttgccg gtacgatgat cattccatcg gtcaaaaatg atccgctggc gacagcagca
      961 cctaaatctt tgtttgaata atggtaatca aacaggcttt cgaattcagt tagtgataag
     1021 tcggattcca gctttaagtt ctgttttgcc catggactga tgattccgtg tgtttctatc
     1081 atcggatatt cttttagttt cttcactagg ttgatggcat aaatggtgcc tgaagcgcca
     1141 cttacaccaa tcacgatttt tttcttccgt tgtgccaatt gttccatttt ccgcactcct
     1201 ttttatgatg tcaaaacagt tgcagatcat tcattatttc aaatattttt cccaatcttc
     1261 tacttcttgg aattttgcac gtacaaaatg ttctttcata tcgaaaggaa ctgtaccgtc
     1321 aaagatagtt tttgcactta tcccacggaa acgaatagag ccaggattat attctggtaa
     1381 ttctgatggg tccaatggat ggttcctcat tcctgggatc acgatgatat ctttatctcc
     1441 ttggaaacga gtgttgaaag tccaaactac atcgttcatg tcgaaaatat caacatcttc
     1501 atcgactaaa atgactgtct tcaattcttt gaaggctgat aaagcaagaa tagctgcttg
     1561 acgctgaata ccttcatccg cttcgttttc tttacggatt tgcatgatgg tcatcaattt
     1621 accaccacct gcaggaggat tgtagacatt tactactttt cctggaatag ctttatcgac
     1681 caaggcaaga atacttgctt ctgtaggaat accggccata gatacgtgtt cttcactggg
     1741 accaatcgtt gtttgcatga ttggattgtc ttttcggtga gtaactgctt tgactttcac
     1801 tacttgtaca gctggattag catccccagt atagccagga aattctggca ttgcttttcc
     1861 tgagttcgtg ttgatatctt cttgcattgt ttcatttggc atgatttctg cttcaatgat
     1921 gaattcagca cgagcaatac ccacttcatc gactgctata ccatcaacga tttgaatagg
     1981 ttcgttacgc aaagctcctg cacaccatag ttcattgtag ccaagtggag tggtaggtgg
     2041 ttcgaaagtc gtgccaatgg caattgctgg atctaaacca atattgatag tgataggcat
     2101 tggtttattc aaggcttcga attgtttttg gaagtgacca atgtggcgtc cgccaggcat
     2161 gatgtacata ccgatcgtat ctttatcttc tagaaccata cgatggatcg ttacatcgct
     2221 cattgttttt tctggatctg atcctaagac tacacccatt gtgatgtatg gaccagcatc
     2281 ttcaggagta tttgtaggtg caggcaaaat gttgcgaata tcgaagtctg catcggtagc
     2341 tagatgaacg acttcttgtg ctggagcgtc tgctttgtct actttcacag gtttgattgg
     2401 attttctaca gcgtctttca agaaacgacc aagtgttcga taatcatggt gcaaaatttc
     2461 acctacacgt ttacgactag ccattgttcc gatgttcaca cgtgtgccgg ggaaaccttt
     2521 gatattgttg aaagtcatag ttggtccttc ttgagtcgga cgttttactg taccgccagc
     2581 accgatataa cggtaaacac cagataattc tgcttcagga tcgatttcaa tatctgtttc
     2641 gtgatattga cctggaagtg tttttagttc ttcgatgact tttcttaaat catacggttg
     2701 atctgtcatg agagattcct ccttagttgt ctttacctat ctatagtaaa aggtgaaaaa
     2761 gaggaaaaca attcaacttg ttcggtaaag tattctacaa tggaatggat gcgttttttt
     2821 gataatagaa taaactatga tatacttatc actaaaagaa agcgcatact tagtgttgga
     2881 ggaaggaaag tgaaaaaaat ggaggagtca tttcctaaag cagtaaaagt ggaaaatata
     2941 gcgaatattt tgaaagttac atttgaaaac ggagaagtaa aatacgtgaa atctcattgg
     3001 acagaagaga taaccgatgc cttgcaattt ggaaaaaaag gaagaggaaa aagaaaaaat
     3061 cttctcgcac tttctcgtaa tatgtggatt gggactgaag taacgattga agcagatgga
     3121 acagtattca taaatggaaa agacaggtat acgccagaag agttgtggta caaaggaaag
     3181 aaaagtatac cagaattata aaaaataatt aaaaaaaaca gcgcgaaaca aggaacatat
     3241 tacttcctcg tttcttgctg ttttctacta tttcagcgct cttatgactc aaatctaaat
     3301 gtggttgtca caaccttatt ctgcacgcaa agcgatagcg gcgtcttttt tcgctgccat
     3361 tcgtgcagga agatgaccgc caagcatcgt caatatagta gagacgatca ctagaataat
     3421 cgcatggaca ggatttagtt gagcaacatt ttttagatcc gtcatgttgt ataagatgct
     3481 attgataggg aacgtagcta gccatgcaat aaacacacct aatatcccag aagaaatacc
     3541 taaaatacac gtttctgcgt caaatacgcg agtgatgtct tttttacgtg cacctaaagc
     3601 ttttagtaca ccgatttctt ttgtccgttc aatcacagat gtataagtaa tgatactgat
     3661 catgatcata cttgttacta aagagatccc tgcaaaagca ataaggacat aagtgatagc
     3721 atccatcaat ccgccagtca gttcagtcat cgttccagct aaatcagtat aaatgatttt
     3781 atcttcatct gatttgcctt tattgtaatc atctagataa tccaagatct tttctttgtc
     3841 ttcaaaattg ttcggataaa tcatgatact tgaaggcaag ctgtctccac ctaaataact
     3901 aagcaatgtt tgtttggcat tttcgtcaac tttttcagta gttaagacat ttacaccact
     3961 gtctctttga gctttgacga tttctgattc tttgttttcg ttgacgattt gagtcgttaa
     4021 ttgatcgcta taagcgattc ctggagataa gagattcatt gtagaagaag atttcacgcg
     4081 taggatcccc gagatcttca attcatcaga agcattctga tacatcgcat cataatctgt
     4141 attagggata aaattcccag ttggaagttt tgtatagtaa gtattgttgt ttactaattt
     4201 gaatgttgta ccaacgatat catcaaaatc aagtttttcg tcttctttca catcaaatcc
     4261 aagattcttc aatgcgttga tattagtatt attattgccg tctacgatca gcacgacatc
     4321 tgttgctgag gctggataac ttccggcaag taaagaataa ttgtctttta aaaagttttc
     4381 tttgctagtg tcaagctgtg tagggaaact tgacactccc acacctgtca ttgctgacat
     4441 cgtactagat aaagaaagag attccgtatc aggattttga ttcgagaagc tgacaggctg
     4501 tactttgcca tttacatcac gtaaaagatt gattcctgta gtacgggtaa atccgatatt
     4561 attacttaag ttaggatcga tgtctgttac gtaatcgacg tatgtttgat caatcttatt
     4621 cgtgtgctgt gctttatctt cttcgctgac ttttgcagta atcgttttgc tgtctggata
     4681 atccgctttg cttgcaccca atccggcatc gtcacgagtc tggttagtcg ttaccttaga
     4741 aatagtaatc ggaaatttcg ccatcgtttc agcttgcgtg ttatcgattt gtttttggaa
     4801 tccagaagaa agggacagaa cgatcgcgat tccgatgatc ccaatactgg aagcaaagga
     4861 ggtcaagaaa gtacgtcctt tttttgttcg gatattgttg aaggaaagct ttaacgctgt
     4921 ccaaaaactc atctttgttc tgcgtaagtt aaattgatca tcttttggtc gttcgatgtg
     4981 aggatgggaa tctgttaaga tcttaccatc agaaaattca atgattcgat ctgcgtattg
     5041 atgggccagt tcaggattat gtgtgaccat gatcactaat ttttctttcg ataactcttg
     5101 gatcaattcc atgatctgga tacttgtttc tgtatctaac gctccggttg gttcatcgca
     5161 caaaaggata tctgggtcat tggctagtgc tcgagcgatg gccactcgct gcatttgtcc
     5221 accagataac tgatttggct ttttatgcat atgatttgtc aagccgactc ggtgcaatgc
     5281 atcttctgcc cgttttcgtt tttcgtcttt agaaacacca cttaatgtca tcccaagttc
     5341 aacgttttca ataatgccta gatgaccaat caagttgtaa ctttgaaaaa tgaaaccgat
     5401 agagttgttt cggtaggcat cccagtcact gtccttaaag tctttagtag actttccgtt
     5461 gattaccata tcaccggaat cataattatc tagtccacca atcacattca gcatagtcgt
     5521 tttaccagaa ccactaggac ctaaaatcgc tacgaattct ttttgtctga aagcgacaga
     5581 cacaccatcc aatgctttgg tggtggtttc gccgacttta taatattttt ttatatcttt
     5641 taattgaagc ataatcaccc ttctcccgta ttgaaatatt cggtatacta ataagtatat
     5701 cagaacaatg atcgtagtat acgaaataaa tgttaactga agatgaacta caagaaaaaa
     5761 tctagaaatt tttcaaaaaa cagagggaga tcaatgaatc gtattttagt agtagaagat
     5821 gatccgcaat tacagttgct ttaccgttct gtgttggaac gagcgggctt ttcagtcatc
     5881 accgcattga atggagagga tgcgctgaaa aaactagaag cgacacgtat agaattaatc
     5941 attacagata tcatgatgcc gaaaatggat ggctatgact tgctagactc attacgtagc
     6001 accagatttg acacacctgt tctaatgatt acggcaaagg ccgattttga ggataagaaa
     6061 cgcgggttca aattaggagc agatgattat atgacaaaac ccatcgatgt caatgagatg
     6121 atcctacgtg tggaagcatt gcttcgacgt tcaaagatca attacagcag tagcttgata
     6181 atcggacaaa caaaattaga gctagatgct tatacagtgt tacaagatca gcattcgttg
     6241 attctgcctc aaaaagaatt tctgcttctg tataaattac tgtcttatcc caacaagatc
     6301 ttcactcgcc aacagttgat ggatgatatt tggggattag atacgaatac agaagaacga
     6361 acgatcgatg tacatatcaa acgtttaagg acccgttttg aaaagacgga agattttcaa
     6421 atcatcacag tacggggact aggctataag gcggttattt aaagatgaaa aaaaagctat
     6481 tttcacaatt atggatctat tttgctagta ttgtcttcat ttcgatctta gtgacactgc
     6541 tgtgttttct cggttttatt ttcttattgt ctcgacattc tatctcgcct gctgaatcgg
     6601 ctggaaaacc aacgttatac ccgttattcg tctttgcggg gttcagtatg gtcgtgggta
     6661 caggaatatc gatttttgtt gggcggcgta tcctgcatcc aatcagtgcg ctagggacaa
     6721 atatgagtct ggtagcaacg ggagattttt cgattcgaat ggatgaacag cagaaagtag
     6781 cagaagtaca gcaactgtat aaagatttca acgttatggt ccaagaactc aatagtatcg
     6841 agacactgag gaatgacttt gtgtccagtg tatcccatga attcaaaaca ccactagcaa
     6901 caatccaagg atatgttcaa ttgttacaag ccccaaatct ctctgacgaa gaacgccaaa
     6961 tatttttata tcggattatc gaaagtatca cgcagctttc acaattaaca gagaacatct
     7021 taaaattgaa caaactggaa aaccagcgta tccaactgga aaagaaagag tatcgattag
     7081 atgaacaaat cagagaagta atcgtttttt ttcagcctaa atgggagaaa gaacagctag
     7141 aattggatat cgaacttgct gcagtaaatt acacaggcaa tgaagagttt ttgtaccaag
     7201 tgtggctgaa tatcatggat aacgccataa agtacaatca aatcaatgga cagattcata
     7261 tcaaactatt tgagacagcc acagagattg tactggaagt gaccgattca ggtgtaggaa
     7321 tgaatgaaga gaccagagat cgaatgtttg aaaaatttta tcaaggagat acaagtcgtc
     7381 aaatttctgg gaatgggcta ggactttcac tggttaaaaa aattctagaa ctgcatgatg
     7441 gacgtattga ttatagcagt atcgaaggag taggaacgac agcaatgata cggttaagta
     7501 aacaatagga aaaagaataa aacccctaaa aattctattg aaggttttta ggggttttta
     7561 gtcgtttttt tgtgattagg gaaatgttct tacggttctt ttggcataaa aatcgctgct
     7621 ttcttattga actacgagaa atgttagagg tgtcattgaa acaaggacat gatgcaagaa
     7681 agagagcgaa agtcatgcgt cgctctcttt ttttgggtgg atagatggac agaaaatgta
     7741 gggcttagtt ttcttcttga tttaaatact cccaaaatgt atctgaagcc aaagaatgct
     7801 caccgacttt acgagtaaat gcaagataac tctctgttgt tggagaaata gggagaatca
     7861 cagtattttc ttttaataga ttcttgactg ttttttccat aacaatagca atccccatgt
     7921 catttgcaat catattgatg atcaagtcga ttcttgctcc tttataagag atttttggtt
     7981 caaatccagc ttcgcgacac agatctagta caggttgata caagttcgta gtggggccaa
     8041 ggagcaaaaa gttctccttt ttcagttcgc ttagatccaa tttcttttta ctagctaaag
     8101 gatgattttt aggtagaact gctacaaaat cgtctgtttc agtaggcaac cactcaatcg
     8161 aacttggtgg ggcctggaaa cttctagcaa agtggatcgt gtcactatgt tcattggcgt
     8221 cattgtaagt taattcgaca ctttccactt ctttcaactg gactgtaaat tcaggatggc
     8281 gtttcaaaaa ctcggtgatt ttggaaaaac ctttataatt gatcattgtc ggtatcgtta
     8341 agatatttaa tgaaagattg ttgtctttcg tataggtttc aagcacttct tgcatagcat
     8401 ggtattgttc tacgacattt tttacatatg ggagcaataa gcttccagct tcggtcaatt
     8461 cgatttttct atgggcgcgt tcgaataatt tgacgtctaa ttctgtttct aacgaaagaa
     8521 tctgtttaga aatatttcct tgggtggtga attgtcgatc agcagttttg gtgaaattca
     8581 ttgtttcggc taaatcaaga aaaacttcga atttatggat atccatcata ttcctcctac
     8641 ttattccaat aaagaatact ttaacacaaa aaatgaattg gtgtaatctt ctctgtctgt
     8701 tacaatcttt cttgcagcga aagcaataat tacagaaatt tttctgattt ttttcgcttc
     8761 gtagaaaaag agaggagttt tttgatgcca actggagttt tgatcaatgt aggttccgtt
     8821 ttgtttggcg gactaattgg cggattagta ggaaataaat taagtgaaca ttttaaagca
     8881 cagctgacga tggtttttgg tgtgtgttcg atggggatgg gaatctattc cattgcgccg
     8941 atgaaaaata tgccggctgt tatttttgct ttagtgatag gaacagcgat tggtttgatt
     9001 gttcatttag gtaatgggat aaataagggg gctgcgctga tgcaagtacc tatttctaaa
     9061 atcttcccta gtgaaaagct tggtatgacc cacgatgaat ttatcagcac attagtgacg
     9121 gtcattgttc tattttgtgc aagtggtacg gggatctatg gaagtttaga ttctgggatg
     9181 acaggagaca gtacgatttt gatttctaaa tctgtccttg attttttcac agcagctatt
     9241 tttgcgtgta atttagggta tgtcgtttct gtgatagcaa ttccccaatt cattattttt
     9301 tatattttgt tcttattggc aaaatttatt tatccattga ctacaccaga catgatctta
     9361 gattttaaag cttgcggcgg attcttgatg gtcgctaccg gttttagaat gatcaacgtg
     9421 aaaatgttcc cagtagctga catgatacct gcaatgatcg ttatcatgcc actgagctgg
     9481 atgtggacaa attggattat gccactgtta taataaaaat caaagagaag ccattttttg
     9541 ctgactaaaa aggatcaaat cttttagtca gtttttttgt atttgacaaa ctgattttga
     9601 aactatatta tatgttgtat aatatagatt gaaataaaat attacacttg agaatagaag
     9661 gtgacgtaat gcacatccag attccaactg ttcttcttga tggtactgtg ctagcagcat
     9721 taagcaagga agatatgtat gggtatgcct taacaaaaat cgtacaagaa agtctctctg
     9781 tcagtgaatc tacactatat ccggttttga gaagattgaa aaaagaagga tatcttgaga
     9841 cctatgatga accttttgaa ggcagaaacc gtcgatatta ctcgttgaca ccaatgggaa
     9901 gtcgtcattt agaagaaatt attgatgaat ggattgtttt tcgagattct ttagaccggt
     9961 tattggagga gagcacaaat gaatgaatat ttgaatgaag ttgtggatca tcttcaagaa
    10021 attcctgaag acgatcgttt tgatttgatc cagtattatg aggaatattt tttagattct
    10081 ggaaaaacat tagatcagat cattgatgaa tatggtacac caaagcagtt tgccttaaaa
    10141 ttaaaaatca gttacttttc agaagaagaa caagcagaaa acgagttagt gaaaaatcca
    10201 aaaagacaaa ttcgcctgat ttggttaatc attatcggtt tatttgcttc tcctgtcctc
    10261 attccactag cactggggtt tatattggtt attgggggac tattggttgc tttactagcg
    10321 ctcattttta gtatctatgt tttatgtatc agtatcttag gtgttggcct attcatgatc
    10381 atctcaggaa tcagtgtgtt tggacaatcg attctgagtg gattattctt tatcggtgtc
    10441 ggtctgttcg ccacaggaag cgcagtattt atttcgccac tgttgctgaa aggaacaaaa
    10501 gggctttttt atgtcatgat gaactttgtt aaatggatgg gacgacgttt tattacaaaa
    10561 agagatctgc attcagcggg cgtttgaagg aggacacata aattgagatt gaaacatttt
    10621 ttgattaccg gtattggtct gatgcttact ggtggcctcc tagcaggtgc aacttataca
    10681 ttaggcgctc aaaagagtct gacttgggat aatggtcctc ggttgatcga tatggttact
    10741 gagacaaaaa aaatcaagga tgcaaacaaa atcgtcatcc atgcagaaaa tcaaacagtt
    10801 tcggtcatac gcggattgga ttttgagata aaaacgactt atgataagac agaaaagcca
    10861 gaaataaagg agaaaaatgg cgtgttgact gtgaatgcag tcaaaaaatc aacaggtgca
    10921 atcattagtt tagaagacac aagtgcacaa atcactattg ttgtccctgc agatcatgaa
    10981 ttatctgaat tgacaatcga agggaataat agtgcaattg atatcaacga agtatcaatc
    11041 aaaaaagtga atactaagac acatgatggc tgggtatcgt tcaatgaatt tacaggagga
    11101 acaataactg cgaaagatga attaggctca cttcgattag atgaggccaa tgtggataac
    11161 ttgatcgtca caggtgaaaa cagcaacttc catctagaag acagtttgat cagcaaagaa
    11221 ggaaaagtca tcctaaacgg tggcaattta tctttgcatg agatggaaaa cagtggctat
    11281 actatccaaa cgtctggtga atcttatatt gaaaaagact accagccaat aacaaatact
    11341 ttgttagaac aaggacagaa aaagctaaag atcactgcta aagacacatc gatcaatatt
    11401 acgacggatc aagaggacaa tgaaacagat tgatcctgta ctcaactgga tattttaaga
    11461 aaaaacagca aagatccttc caatctttgc tgtttttcat cgcttaacgc acataactat
    11521 agtaaaataa gaaagaaaat atctaaaaag aaaacagggt gaaagagtat atgaaggtag
    11581 aagaaggtca aagagaaaag ttaaaaacag aaatggaaag attacatact tatatcactc
    11641 aactaagcca aaccttctat gatccagaca aagaaaaagt gatggttaat tacccgaaca
    11701 attcagaagg tcgccaatta gagcaagtgt accatgaagt tttcaaacat ttattgacgg
    11761 tgaagaaaga gctggattat tattctttac cgattatcga tacaggaata ttgaagtatg
    11821 atgggaagaa ggaacgattt atctttaaat ctgttcggga agatttgcct ctttcagcag
    11881 ggatggatct agaggtcttg gtagaagact attttacaga agaaaagcac tgggttcgaa
    11941 caaaattgga ttatttaccg caggcagccg gagggacaca ggcaagcggt tggtacatca
    12001 ctgaagacaa agagttagaa ctagaaggtg taatggcgcg tattcgaaaa aaaaattaaa
    12061 aaagtaacat tagatattta ttgaaggata aaggagcgca ggaagtagca tgagagaaaa
    12121 aagagcattt tatatcaatg gttatgcagg gattgtcgga ttggtcgtat tagctttagt
    12181 tggattgttt ttcttctatt taggtatgtg gcaagcgaaa gtttgggcac tttttttaag
    12241 tatttttctt tggttgatca cgttattgtt gttaagttcg gcaactgtag tcagtcctaa
    12301 tcaagcaaaa gtaattttgt tttttggaca atatttaggt acgattcgag aaaatggctt
    12361 tttccttacg atacctttgg cacaaaaaat gactgtttcg ttgaaagttc ggaatttcaa
    12421 tagttctgtt ttaaaagtca atgatttgga tggaaacccc atcgaaatct cagcagtagt
    12481 agtctttaaa gtcgttgata cagcaaaggc attattcgat gtagcgtatt atcaagattt
    12541 tgtagaaatc caaagtgaga cagccattcg gcatatcgct agccaatacc cgtatgatac
    12601 attcaatgag aatgatttga cgcttcgagg aaacacgacg gcagtgtctg acgagttgca
    12661 aaaagagctg caagagcgtt tagcagtagc aggtgtcgaa gtaatcgaaa cacgcttgaa
    12721 ccatttagcc tatgcaacag aaatcgctag tgccatgctc caacgtcaac aagcaaaagc
    12781 cattttgtct gctcgacaaa ccattgttga aggagctgtc acaatcactc aaatggcctt
    12841 agagcaaatc gaagatggac aagaaatcaa tttcaccgat gatcggaaag tccaattgat
    12901 caataattta cttgtctcaa tcattacaga caaaggaacg caacctgtta tcaatacagg
    12961 cgacatgaaa gaccgtaagt gacatacttt ttgtaagata ggaagaagga gtttaggacg
    13021 gattattgtc ttgaactctt tttttagttg ttttaaagaa aatgacaaaa ttgaaagaag
    13081 aatgatagtt gattcgaagg cagaattgtt cgcttttttc tataatcagt atataaagta
    13141 agagagaagg ggaaaaagtg aaaaaacttc tgatcggtct acttgttttc atcgttgtgg
    13201 tacttgcagg ggtgaaaatc gcagattacg tagttatggg tggagaagaa tactatgtac
    13261 aaattacgac agatggtaca aaaacagttt caaaatcaga ctctggacaa gaaacagtca
    13321 actatagata tatcctgcct ggctatacga aagagggtaa ggaaaaagaa ctggacttca
    13381 agggacaatt gactcgaccg cttcgtaaag gagcatattt aaaaatcacc tggaacaaaa
    13441 ataaaggtgt cacttcgtat gaagaagtcg aaaaaaaaga cgtgccgaaa acagcactgg
    13501 aaaaactaat taaagggtag ggaaagaaat gaacaaaata gtcgaagtac agaatgtaac
    13561 aaaagtatac ggaaaaaaca atgagaaaaa gacgcaagca ttaagcggca tcagcttcga
    13621 tgtagaaaaa ggcgaattta ttgggatcat gggcgcttct ggttctggta agtcgacact
    13681 tttgaatatt ttatccacat tagataaacc aacagatggg catatccgaa tcaatcaaaa
    13741 agatgtgacc acattaaaag gaaatcaact agcagatttt cgtgcgaatg agattggctt
    13801 tattttccaa gatttcaatt tattagaaag cttgacagca caagaaaata tcgctgttcc
    13861 tctttcttta caaggcgtca gaccaaaaga aatcaagcaa cgtgtccaaa aaattgcaga
    13921 acgtttatcc atctctcata ttttggaaag ctatccttct gagatttccg gtggacaaaa
    13981 acaacgggtc gccgccgcac gtgcattgat cacacaacca acgattctat taggggatga
    14041 gcctacaggt gcgctggatt ccaaaagtgc ccgggatctt ttagatacga tggacgaatt
    14101 gaatacaaaa gatcatgtat cgattcttct tgtgacgcat gatccgtttt ctgcaagtta
    14161 ttgccaacgt attttattca tcaaggatgg cggtatccat caagaagtga aacgtggaga
    14221 tcaaagccgt gattccttct atcgagaaat actatctatt cttggaaact tggaacagta
    14281 gggggagatt gactatgctt tggaaattat cacttactgg gatcaagagc cgtttaagag
    14341 actacttggt gttattctca ggattagtta tggcatctgc tattttttac atgtttgaat
    14401 caatggcaag caacgaagct tttttgaaaa gtaatacgat cgttgcggct acagttatca
    14461 ttttccgatt cggttctgtt ttactaggga tcatcacatt cgtttatatt ttatatgcaa
    14521 attccttttt gatgacgatg cggcaaaaag actatgcaat gtttatgatg ctgggagcaa
    14581 aaggcagaaa aatcgcacag atgatttttt ctgaaacatt cttagtaggg atcgcagcta
    14641 ctttagtggg ttcagctgtc ggtgtaggat tgacaagtat cgtccaccga ttattagtgg
    14701 atcagttaga tctcaatatt tcgcatttta caccttttag tattcacggg ttgttgatca
    14761 ctgttttgtt ttttgctgtt ttgtttttat tagctgctgt tgtcaatgct ttttcgattg
    14821 tcaaaaaacc gattttagca ttgattcgtg cggatcagac accaacaaga atcaagcagc
    14881 ataaattctt gtttttcctt gaagtagtac tgggaattgc ttttctaggc tgcggttact
    14941 atatgatgaa acacgtgggg acctatcaag tctttggtat aggggtagcc ttagttacga
    15001 tcgtattagg aacttatttc attttccatt ctgtgatcat tttcttttta tcattactaa
    15061 agaaatcaga acagatttca ttgaaaaaat tgaacaattt cacattgtca cagttaaact
    15121 ttcgtattcg tgaatataca caaatgctct caatggtcgc tatgctgttt gccttggcac
    15181 tgggcgcttt gacggtagga ttaggtttta aaaatgaaat tgcagacttg acagaaaaca
    15241 attctggata tgatctagtg atgaataatc ctcgggcaga agatcagcaa aaaatcaaag
    15301 aactttcacc aacactttct gcttcttata ctcaaaaaga agatgcggga acgatttact
    15361 ataatgcaag tgaatttgcg gctcaaccat tgatcatgat aaaacatgaa gatggaacac
    15421 cgccgaagat cacgaaagaa aaagttcctg ctaaaaaaat gaatgaactg actgtacaag
    15481 aggaacttcg aagctatgaa ttgccggaac agcaagaaaa agaaatcaag gtagtcagtc
    15541 aatcagaatt tgatcaactg aacttgccac aatccacttt acagctagtt caggtaaaag
    15601 acttccaagc aaacctgaaa ccaatcgaaa cacttgttgc tcaaaataaa atgaacaatc
    15661 catctttgca aaaagtagag tacagcaacc agaaatatga aatgtatgaa gtgtataatg
    15721 gtctattctc tggttttgaa tttatgggat tcttcctagg tttagctttc ttgaccatgc
    15781 ttgctagctg tttgatgttc aagatcttgt caggaagcaa gagtgacatt gctcgttatg
    15841 aaatgcttga aaagatagga acacgacgcg ggttgcttaa acaatcgatc cgtagagaag
    15901 taggcgtgtt attcttagca ccaggaatct taggtgtagt ccatgtctta ttcggattac
    15961 aaatgtttgg tctattcatg caaaatccat acaaagatat ttgggttccg tttacgatat
    16021 tcttcgtatt gtatttcatc tactatgtac tgacgacatg gttatatact ggaatcgtat
    16081 tgaaaaagag attgtgaaaa agctgtcctg catcaagtaa taagaacagc caagcaaaaa
    16141 tag
//
LOCUS       DACECR010000066        16085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.066, whole genome shotgun sequence.
ACCESSION   DACECR010000066 DACECR010000000
VERSION     DACECR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.066"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(91..1509)
                     /gene="nhaC"
                     /locus_tag="IWO17_001922"
     CDS             complement(91..1509)
                     /gene="nhaC"
                     /locus_tag="IWO17_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0290790.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1913..3136)
                     /locus_tag="IWO17_001923"
     CDS             complement(1913..3136)
                     /locus_tag="IWO17_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ0290791.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3168..4007)
                     /locus_tag="IWO17_001924"
     CDS             complement(3168..4007)
                     /locus_tag="IWO17_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ0290792.1"
                     /translation="MKKNIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(4024..4704)
                     /locus_tag="IWO17_001925"
     CDS             complement(4024..4704)
                     /locus_tag="IWO17_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0290793.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(4706..5779)
                     /locus_tag="IWO17_001926"
     CDS             complement(4706..5779)
                     /locus_tag="IWO17_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0290794.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     gene            complement(6429..7100)
                     /locus_tag="IWO17_001927"
     CDS             complement(6429..7100)
                     /locus_tag="IWO17_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0290795.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(7188..9866)
                     /gene="mgtA"
                     /locus_tag="IWO17_001928"
     CDS             complement(7188..9866)
                     /gene="mgtA"
                     /locus_tag="IWO17_001928"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ0290796.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWLFITVVAYLALVTAVKKIYIRRFQELL"
     gene            10526..10963
                     /gene="ndk"
                     /locus_tag="IWO17_001929"
     CDS             10526..10963
                     /gene="ndk"
                     /locus_tag="IWO17_001929"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ0290797.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(10985..11851)
                     /locus_tag="IWO17_001930"
     CDS             complement(10985..11851)
                     /locus_tag="IWO17_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0290798.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(12085..14163)
                     /locus_tag="IWO17_001931"
     CDS             complement(12085..14163)
                     /locus_tag="IWO17_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0290799.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            14656..15873
                     /locus_tag="IWO17_001932"
     CDS             14656..15873
                     /locus_tag="IWO17_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0290800.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
BASE COUNT         4697 a         3468 c         2777 g         5143 t
ORIGIN      
        1 ggcttattac cgcaaatgca agcttttgaa caccgtttat tcgtaaaagg gagtgtgaca
       61 aaagtcttgt cacactccct ttttacgttt ttattgttct aattgcgctg tcttgcttac
      121 tgcttcttgc ggtttcacaa atttctttga gatcccccaa cctgtaaagg cactgaccat
      181 cgaaaacaca ggtgaaagca aactgaaaaa cacgaatggc aagtaagtga gtgtaggaat
      241 gccaaatgta cttgctacga aacttccggc gattccccaa ggcactaagt agttgattac
      301 tgttccacca tcttctagtg ttctccctaa tacagttgga tcaaatcctg ctttttcgta
      361 agtttcttta aatgcattac caggcaggat aacagataga aattgttctc caataaagat
      421 atttactccg atgcttgtca tcaatgttgt aaaaatcaat tttggggtgt tcgtcatctt
      481 tcttgatact cgttccataa cggttgagat caacccaaat tcgatcaaca aaccacctaa
      541 tgacaatgtt agaataatca atgacacagt tggcatcatg ctttcgattc ctccacggct
      601 aagtagtgaa tcgatttgtt ggtttccagt atttgaaaca aatcccgatt cgatgacccc
      661 tgccaacttc gttaaggaag tttgtgggtt ttgaataaag atcattccta ctgctacagc
      721 aatattcaac aaaatcgtca aaattgccgg tacctttttc catgcacaca agagcattaa
      781 aaatagagga atcagtgacc agatcgaaat agtaaagttt gcttctaaaa tatcagtcac
      841 ttcacgaata gatgaaagac ttgcggcttt acttgtgtgt cctaaaatca tgaataaaaa
      901 gaaagaaaca acaaatgccg gaacagttga ccacatcata tgcttgatgt gtttgaacaa
      961 atcggcatca acaatagctg ctgctaaatt tgttgattcg gataatggag acatcttgtc
     1021 tccaaaaacg gcacctgaaa caatggctcc aacaacaagg gctggattta gtcctagtgt
     1081 tgtcccaata ccgaaaaatg caatcccaat cgttgacata acagtaaagg cactccctac
     1141 agcgctacct acgattgcac aaacaacaaa tacagaaggg acgaaccact ggacactaat
     1201 caatttaaat ccaatgacca tgagggttgg aataattcct gcttgtatcc atacagcaat
     1261 caatgaaccg actaaaataa aaataaagat tggaatgatc cccggtttga tgccattgat
     1321 gatcccttcg tgcagccaat ctgtcgggta tttttttatc actgcgtaca acatgaccat
     1381 tccgatagct gccaaaatcg tgatattagg tgaaaccttt aatccgacaa cgccaatcgc
     1441 aatgcctaaa acaatgacca tcaatacaag tgttgcttct aaaaaacttg gtgttccttt
     1501 tttttccatg ataatctctc cttttttagt tgttcttatg ttctaaaaaa gttaagatat
     1561 cggattactc cgccacaatt catccgcgat tcctccttta taaaaataaa aagacctgct
     1621 ttcaactaag gacgaatgac tccgcggtac caccttaatt aaaaacagat ctgtttttca
     1681 ctttcaaacg attattcaaa tgtatggact ttccatgtaa ttcgtcaatt aggcttgatc
     1741 ggttcacagc gaccaccgac tttctgaaca aaacacctaa ccactactgc gtgaaaagtt
     1801 gacttgtatg aagtttacct tccataagat catttgtcaa tgaaaaagaa aatatttttt
     1861 catttttctg aaaattattt ttataacttt cgcgcacaac tttatctttt tactagaaag
     1921 tagcgccctc ttgttttttt cttgctacta tttcttgatg ttcttgtttg attttttcta
     1981 atagcttggg ttctctcaat aaatctagag ctgtattggc aagtaattcc gctgcaatcg
     2041 caatagaagc aagtcctttt tcacttttag cagcagcttt gaattcttct gtatgtccgg
     2101 caatatatgt ctcagagatt gctacagtag gctgtatcgt tggaataact tggctaacat
     2161 ttcctacatc agaagaacct agacttgttc tttgttccgt ttctatttct tcatcaggaa
     2221 cccctgcttc ttttgcatgt tggaagaata agtcatcgaa agaaggcgtt acgatgacat
     2281 catcgactgc attttggaac aatccaaact caaaagtcgt acccgtgctt aatgcagcac
     2341 ctcgtacgat attttccact ttttcatata ctttgtctaa tgtttgtcga tttcctgcac
     2401 gtaaatagaa acgtccagca gcgtagtctg gtatgacatt ggccgccacg cctccatctg
     2461 taatgattcc atggatattg acatcttttg gcaattgcaa gcgtagagcg ttgattccgt
     2521 taaacacttg gatcaaggct tccaaagcat ttactccttt ttcaggtgct gctgctgcat
     2581 gagaagcgac accgtaaaat ttgatatcga ctggatcatt tgctaaagat tctgtcgttt
     2641 tcccataacg aaaagctgga tggacacata atgctgcatc gacatcctca aaaaaacctt
     2701 cccgaacgaa gcttccctta gctgatccat tttcgcctcc ttcttccccc ggagtaccat
     2761 acacacgaat ttctccgcca atctcatcaa tgaattgttt cactatgctg gctgctaata
     2821 cggagtaagt accaaataaa ttatgtccgc atgcatgacc aatacctggt aaagcatcaa
     2881 attctgccaa aaaggcaatt gtcggacctg gtttattgga aatataacgt gcatcaaagc
     2941 ctgttcggtg cccagctact tctttttcta cccagaaccc ttctttttcc aattgtttga
     3001 ttaaagctgc tgaggaataa aattcataat tacttacttc aggatggtcg tggatagcta
     3061 gtgcaagtgc ttgatattct ggtaaacggt attgaatcag atccttcaat tgatttgttg
     3121 agagtgtggt tgtcataaaa atttcctcct atgctagtcg ttttttctta ttttctcccg
     3181 aatgtttccc acgcaggtac gcttgaacct tttgatgttt tttcgattac tttggctgtt
     3241 tcttctgttt gataggcatc tactattttt tgatacgttt tattgtcttc atcttctttt
     3301 cttgcaacga tgatattgac ataaggacgt gcatgctcat cgactggttc taagaagatt
     3361 gcatcagaag cgggattaaa tccagcatct acagctacac cgctattgat gactgaaata
     3421 tctacatctg acaaagcacg tgctgtttgg gaagcatcta actcagaaat gtctagattt
     3481 tttttattct cggtaatatc agaaacggta ggcgcttggc cttttgcctt gtccactttg
     3541 atcaatccag cagtttgcaa taacagaaga gctctgccgc cattcgtgac atcattaggg
     3601 attgcaactt ttgctccatc tttgatatca gccacatctt ttaacgtatt ggaataaatt
     3661 cccaacggtg cattaaccgt attcccaatc gatactaaat cagttccgtg ttcttcattg
     3721 taattgtcta aaaagaattg atgttggaag gaattcaaat cgatcgatcc atctgctaaa
     3781 gccgcatttg gctgattgta atctgtaaat ttcacatatt ccaaatggat gttttccttt
     3841 ttcaaacggt ctttcacatc gtcccataca tctgtatctt ccccaatgat ccctagtttt
     3901 acggtcgttt cttttttttc agttgtagct gcttttcctt ggctgcatcc tgccaaccaa
     3961 ataattgcta caaatgtaat gactgcaaaa cgaatatttt ttttcatttt ttctcctcca
     4021 ttttcaatgt gtgacttttt taatcagttg attactgata aattgactca aaaaaactaa
     4081 gaccagaatc aataatgtag cgacaatcgt tacatctgtt tggaatcgat tatatcctct
     4141 cgatatagca agattcccta atcctccggc acctactgct ccagccatgg tagtcagtcc
     4201 gatcacattg atgatcgtca acgctgagac ccgaatgata ccaggtaacc cttctaacaa
     4261 ataaacacgt aaaatgatgc cgattgggct cgtgcccatc gcttctgctg cttcgatcac
     4321 cccaggatca acttctagca gtgcattctc gatttgacgg gcaaaaaacg ggacaacgcc
     4381 aaaaatcaaa gggactaaag ctgctttggc accaatcgtc gttccaacta agaaacgagt
     4441 agtcaaagct aaaagagaaa gcaagataat gaaagggatg gaacgaatga tattgatcaa
     4501 cttatcaagt atttcgaaca gcagcctgtt agctagtaat ccatttggac gtgtcactac
     4561 taacgtcaca cctaagatga ttccgatgat cccaccaatg aatgccgtcc aaaaagacat
     4621 atatagtgtt tgctttgtcg cttcgatgaa ttcatccgga atcaaataga cattcggcaa
     4681 atatgtttta aaaaattcag tcattttagt ttcctccttc gatgactttg atacggttat
     4741 ctaattcatt tttttgtaaa atagttactt ttacaccaga tttaatcaaa taatccaatg
     4801 ctttttgccg ctgtttggtt tctccagcta aagtaacgat caaacttcct aagggtgttt
     4861 cttgtaatag ctctacatta ccgtataaga tattcgctgt tacttggtac ttgctgtaaa
     4921 gatgagcgat tagaggttcg ttcgtctggt tgccgatata acttaattcc accaaccatt
     4981 tatttgcaat ctgatcggca aaagcagcat gggcactgat tgtttccaat gcttggtcaa
     5041 ggtgtgtcgc tgtccgaata aaatctttag tcaattcttc ttcaggatga ctgaaaatct
     5101 ggatgctgct acctttttca atgattttcc ccgcttccat aaccgctacc ttgttgcaga
     5161 tttctttgac aacttgcatc tcatgagtga tcaggacgat tgttagacct agctgacgat
     5221 ttagtttttt taacagtgca aggatctgaa gggtcgtttt cggatcaagt gcactagttg
     5281 cttcatcgca taacaacact ttaggttcat ttgccagagc tcgtgcgatt gccactcgtt
     5341 gtttttgccc ccccgacagt tgtttaggat acgcagatgc tttttcttcc agaccgacaa
     5401 gttccagtag ttcattcact ttttgacgac gttcctgttt tgattttcca gaatacttta
     5461 gagaaaaatc tacgttgtca aaaatcgttc gactttccat tagattgaaa tgctgaaaga
     5521 tcataccgat ggcctttctt tcttttcgca attcttttgg agagagctgg cttaaattcg
     5581 tctgattgac gatgacttct cctgctgttg gtttctgcaa caaattcatc acgcggacta
     5641 acgtactctt tcctgcacct gagtaaccga cgatcccata aacatcccct ttttcaatcg
     5701 ttaaatctac agaatctact gcctgtatct ctttttcttt ttgtttgaaa catacagaaa
     5761 tatctcgcaa ttcaatcatt aacattcctc cttgtctata aaaaaagcat cctcaagaaa
     5821 atgaaaatag tcattttctt gaggacgcct ttttgcgtgt taccacctca tgttcaagct
     5881 gtgttcacac acaactcctc ttcaagtacc atcatactct agcaagatat cggtcgcagc
     5941 cgtcaccgcc ttacatctat gctcgggatg aattgctcga tggccatttt ccaatagtcg
     6001 ttccttgctt cttctcagct accgaagctc tctgtcaaag gttcttctat cgtacttacc
     6061 atttcaatgc aaataatatt caaaaaaaaa atccttttac taacataaac tatgtcagta
     6121 aaaggacgac gtatcgtggt accacctttt ttcggaagtt tccttccctc aagcccaaca
     6181 tctcaaaaag agagattggc gttcgtttta acggtcgaac atttccgaat ttgcctactt
     6241 atcgacaaat tattctccaa ggccattttc agcttttctt acttgactct tcacaccagc
     6301 cagagcctct ctgcaaagtt tccaagctta ctttccttct caacgaaatc aatattttgt
     6361 tgttgggaat aatctacagg aggaaaactg aaaagtcaag catgaaatcc aaattatttt
     6421 ttctatattt atttttctgt ttttttatct aacatttcaa taacagttcg aagatctttt
     6481 ccttccgcaa gaaggttcaa tttttccttc tcttcgacgc tataatttcg aacaaaactg
     6541 gaatcgggaa tcaaaacaac gcctagtcct gcacgtgaag cggctgtcgc tcctactaaa
     6601 gaatcttcca ctgcaaaagc tgaaagagct tctaatcctg ttcgttccaa agctgtcaga
     6661 taagggactg gtgaaggctt gttctccgat acttcatctc cgaacgtaaa cgtatcaaag
     6721 taatcggcta attgaaagtg atccaagata tctgttgctc ttttcgtaac agttgtcgta
     6781 gcaatcccta gtttcatatg acgttctttt aagtctgtca atatttcttt tgcatagggt
     6841 ttcaattgga tagctccact tgtcagctgt tgataaaaat attcttcacg ctttgctcgg
     6901 acttcctgca ctttttcagg actgcctgct gcctgcacta aaatatcaaa actttgtttc
     6961 cagttttttc cgccccaagc gtcaacgatt tctttcggga tatgaatatc aaattgctgg
     7021 aatccccaaa gccaaccatc ccgatacgtt ctttccgtat ccactaacaa accatctaaa
     7081 tcaaatattg cacctttcat ctatacatta atcctttcca aaacaataca cctagtttag
     7141 catgactgat ccaaaatgaa cagaagcata gagcccagcc gcatcactta taagagttct
     7201 tggaatcgac ggatatagat tttcttcaca gctgtcacaa gcgccagata agcgacgact
     7261 gttataaaca accataacca gtaattgcca gggagcggca tcaagcctaa ctcagcacca
     7321 aatcctgtga atggaaggac tgaaccgaca atgattccta atgtagtgat aaccagcatg
     7381 gcaaatgttg cattgctttg gataaatggt attttcggtg tacgcaatgc atggagtact
     7441 aatgtttgtg tccacaggga ttcgacaaac catcctgcat ggaataatgc gataaaaatc
     7501 acttgttgtg agctatccaa tgtatggaaa cttccaccaa ctacagcagg acaaataaca
     7561 aaatacatca acaagtaagt agtgatatca aaaatcgaac ttgttggacc aaaataaacc
     7621 atgaattttc caatactaga tgcattccat ttctttggct catccaaata ttctttatcc
     7681 atccgatccc atggtaagga aatgcaagaa acgtcataga tcagattcaa aaatagaagt
     7741 tgcaaaggca acattggtaa aaatggtaag aacgtactgg caatcagtac ggaaaacata
     7801 ttgccaaaat tagaacttgc tgttgccttg atatatttca tgatgtttcc aaaagtttct
     7861 cttccagaaa gaaggcctct ttccaaaatc gtcaaatctt tttctaaaag gatcacgtct
     7921 gctgattctt ttgcgatatc cacagctgta tccacggaaa taccgatatc agccactttc
     7981 attgctggag catcattgat accatctcct aaaaatccga cagtatgccc atttttacgt
     8041 aaagctgtcg tcaatcgtgc tttttgctga ggattcaatt ttacgaatac ttcgtgttct
     8101 tcagctactt gcgctaattc atgatcattc atttccgaga ttttctcacc agtaatcaat
     8161 tcattgattt ctaggcctac ttgacggcac acagaccgtg tgactaattc attgtcccca
     8221 gtcagaactt ttacagctac accatgttct tttaaagctt tcaacgcttg ttttgttgtt
     8281 tcttttggtg gatcaaggaa agcaagataa ccaatcagaa ccatgtctga ctcatctttg
     8341 actgaaaatt ctccaacaac gcttggattg gtcttttgtg cgacaccaat cactcgtaag
     8401 ccgtcttcat taagtgattg tacttttgca agcaccgctt cttttcgttc ttttgttaat
     8461 ggtgcgacag taccaccaag atcaataaat ttagaaatgt tcaacatttc ttcaatcgcg
     8521 ccttttgtga tcatctgtgt tttgccagcc ggatcagcga ctacaacact catacggcga
     8581 cgttcaaaat caaaaggaat ttcatctact ttttgataat tgattgtatc tgtatctaaa
     8641 gtttctgctg cttcatcaat gatagccaca tctaacaaat ttttcagccc tgtttgatag
     8701 tagctgttta gataagcatg gcgcaaaaca cgatcatctt catttccttc acaatccata
     8761 tgatattcaa gaatgatttt gtcttgtgtc aatgttcccg ttttatccgt acataacaca
     8821 tcgatcgcac caaaattctg gatagcattt aaatttttga tgactgttcc ttttttggcc
     8881 atcacacttg ctcctttgac caaattcgtc gtaacgatca ttggaagcat ttcaggcgtt
     8941 aaaccaactg ccacagataa tccaaaaaga aaagcttcta accaatctcc ttttgtaagc
     9001 ccattgataa cgataactgc tggagccatc aatgacataa atttgatcag cagcatggat
     9061 gttttgtgga tgcctaattc aaaacttgat tggatgggtt tttcagatag cgtttctgct
     9121 acatgcccaa atagtgtgtc atttccgaca gaaacaatga ttccttccgc gcttccactg
     9181 atgacattgc ttcccataaa gacaagattt tcataacttg tttcactttg ctgatcgtct
     9241 tcacatactt tcgcatgctt ttctactgga tagctttccc ctgtcaatgc tgcttgagaa
     9301 atgaatagat cctttgcgga caataaacgc atatccgctg gtatcatatc tcctgctgaa
     9361 agccgaacaa gatctccaca gacgatttct tccataggaa tctctgtata ctcgccattt
     9421 cgtttgacag ctgccgttac tttcacaagt gatttcagtt tttcagctgc ttgattagaa
     9481 cgcacagact ggatcaacgt catcgtccca ctgacgataa ccatagtgaa gataatggct
     9541 acaccaaaga gatctttatc tcctggagcg gctaatacgt attccgtaat gaatgaaata
     9601 attcctaatg caatcaacac taatgtaaag ggtgtgatgt atgctttcaa tatttctata
     9661 atgaatggtg tttttttgcc atatgaaatc gcattttcac cataagtttc tcttgaacgc
     9721 tcgatttgtt cttctgacaa tccatttggt gatgcatgga acaattcaaa taattcttgc
     9781 ttcgtttttc cagcaaaagc tgcatagatt ttttgtgtat ctttcttttt ttccatctcg
     9841 atctctcctt tgtgtccaaa agacagctgt tctcgctgtt tttggtcttt tgctattcat
     9901 cggatcgata gagaaaatgc tgaaaggggc tgagaacaga tagataatag ccctggcctt
     9961 tcctctaaat ccttgttttt cgaatgactg tcatccattt gttctcatct cctttactaa
    10021 gtaattttaa ttcatgactt ccaatcattt ttcgaatagt tattttccca actcgctcac
    10081 ctcccttttt gttttttcac ctatgggatt tgcggcaaca aaaaaacctc tcgccaaata
    10141 ctcagcgaaa ggtttgaaaa agcaaatcaa aaaaagcttc tcagatctca tcgttgagtt
    10201 ttgacactgt acaacgtaaa aagcagatat ctgcttttag ctaccttatg aaaagcctta
    10261 tctcgacaat tcctgttcta cccattggcg tctctcgacg tttttgggca gtagcctatg
    10321 tttgtatagg agcctcacct aacaaatcct attcggtttt ccctcgttaa tataacaact
    10381 aaaaaagagg atgtcaaatc aattttccat gtttttcata cttttttaaa atacacgcgt
    10441 tatatttcct gtctcacgca gttttatttg tctcattttc cacctcccgc tataatagaa
    10501 ttatagtagg aaacgaggtg ctgttatgga acgaacactt gttattatca agccagacgg
    10561 cgtaagacgc catttggtcg gaagcatcat ccaacgtttt gaagcaaagg gactggccat
    10621 tgctgaaatg aaatttgcaa cgatgacacc tgaactagca aaagaacatt atcagcactt
    10681 gactgaacgt agtttttttg atgagcttat cgattacatg acttctggtc cggtcgttta
    10741 tcttgtgctg gtcggcgagg aagtgatcga tatcgtccgt aagatggtcg gagcaacaaa
    10801 aggggcggat gccgttccag gtacgatccg aggagattat gcactgccgg ggactgaaaa
    10861 catcatccat gcttctgatt cccgtgatgc tgcaacaaaa gagatcgccc gctttttccc
    10921 gccattcgat acaaacaaac cgtctgaaaa agcggcgaag tgatacaaat cctaaactgt
    10981 tggtctaact gctgggttta gcaatggcca gcagtttttt ctctaaagaa ttcctttgat
    11041 tcttccctaa tgtatattcg atcactgcga aaaactcttg gtgattttcc gatttttctt
    11101 cccctaaagc agcagaaatc agtcgttggt aataatccgc catcaatgtt tcataaatca
    11161 ctgtacttct ttctttatgc gctaaactcc gtttcatatc ttcattcacg ctacgtgcct
    11221 tttttaagcg accattttct atataaaaca aagtcaagtt cgtcaaaaaa gtaaagagat
    11281 actgctcgtg attattcagt tgcgcgatcc gtcgcattct ttttttgatt ttcaaataat
    11341 tattctcaat aaaacgatca ctgaatatcc aaggaaaatc tgctagaata gatacttcaa
    11401 attgcccata ctctggtact tgcatcaaat attcttcaat gatacgacat tcataacgca
    11461 aaaattcaaa ttccgtctca gatacttctg tttttttgga catgtgcaga ttcaaaacca
    11521 aatagtacag atcttctgtt ttcttatagt gcctgcggtt attagcaatt tctttgtaag
    11581 gatcaaagct gtcttcttga atccgatcta gcattcgtct ttttttcttt aatttttttg
    11641 gctcatcaag ctgatttcgt atccattcat attcggcaag tgtcatgttc attcgctcta
    11701 aaaatgattt caatgttcca aacgatactt ttttcactga atttgtctcg atcccgacta
    11761 gcgaagtttt agaacagatt ccttccgcta attgggtctg tgtcagcttt ttgttgactc
    11821 gaagcttctt aatcaattct ccatcataca taatagttct ccttttttaa ttgaaattaa
    11881 cccttggctt ttccagaatg cttgccaata agaggaatga ttatttcaat ttaaattttc
    11941 cgcattcaat aaatagatac gttaaaaata aaaaatacac gaaaaaaata aaaaaatgtt
    12001 tttctcataa aaaataccaa ccttcaagca tgagactgct gtctaatccc tgaaggttgg
    12061 tttgattgtt tattgatcat ttttttactg ctccgcattg gttcctgtta ctggatatag
    12121 gatagtcgtt gttttattgc cgttttttga aaagacactg gtcgaatgtt cacctttttg
    12181 cttttcgatt gcttcattac gttccgcttc gtttttatag tcatattttt tcggatcaat
    12241 cgtctcaaat ccgtttggca cataaaaccg caagagattt tcctgattca gcttatctga
    12301 aagacttaac gcagtcttga ctttttcctg atctttggag atttcctcgt cgattcctgc
    12361 tgctttcgta tcgatgattt ctcctgtctg attattgtaa gcttttcctc ctaggacggt
    12421 gtatttgggc gtcacgaagt tcccatttcg aaaagcaact acttgatcgt gctgagaaga
    12481 taataagtct gtaccaaaat gaagataatc tttatcatcg attcccaata gatgtagtaa
    12541 tgtcggcaaa acatcgattt ctccaccata ttcatgattg actgtccctt tagtgtaacc
    12601 aggcatatgg atcatcaatg gcactcgttg catctgtgcg ttgtcaaatt catcccatgt
    12661 atctggatct ttaccaagag cacttgctaa atctttatta tctgtattcg agatcccaaa
    12721 atgatccccg taaatcacaa agattgaatt ttggtaaata ccagattttt tcaaatacgt
    12781 aaaaaattgt tccaatgatt gatccagata atgggccgtt cgaacataat tatctactgt
    12841 gctattgcca gtattcaaag atggaaagtc gaaattcttg tcatccgtat aataaggcgt
    12901 gtggttggtt acggaaagaa atttcgtgta aaacggctgt tgtagatgtt caagatattg
    12961 ggcagattct ctgaataaat ctttatctaa tatcccataa cctaacgttt catcggattc
    13021 atcaaaataa gatcgatcaa agaaattgtc ataccctaaa tttttatata catgatctct
    13081 attccagaaa ctagctacat tcccatggaa aacagcactt gtatagcctt ctgtttgacc
    13141 aagggtcgct ggtgctgctt gaaagacatt atctgaccca agttctgtga ataatgagcc
    13201 ttgaggtaaa ccaaatgtgc cagtttccaa catgttttca gcatcacttg tctttccctg
    13261 tcctacttca tgaaagaaat tgtcaaaact gatcgttgct tgattctgga aaatactatt
    13321 taaaaacggc gtgacttctt gtccgtctac cttcatattg atcaaaaact gttgaaaact
    13381 ttctaaatga aggacaatca cattttttcc tttagcaatc ccaaatgttt caggatttgg
    13441 ctctgcataa tgtttctttg tatagtctaa aacttcatca attccattac tgcttgcatg
    13501 cgcgcgaaca ctgctagtca ttcctgtttt gattccgtca taaatagtgt acgcatcgat
    13561 tcctagatat ttgacaatat aggaacggtc aaatgtccgc tgtagcaatt ggggacggtt
    13621 ggcttcgctc aacgctaaat ttcctgaaaa aatcaatcct gaaagacagg taacagcgat
    13681 agccatcggt ttgctgacag ggttgctttt gataggtatt ttcttgacct tcaaccaaac
    13741 cagtagccca atgaacacag cgatatccaa ccaatagatg acatcatggg gtttcatcaa
    13801 agaaaaggag cttcctgaca acccttgtga cactttcgaa aagcctaata cagatttgac
    13861 tgtaatgaaa tctgtgaatt cccgataaaa aataatattc aagtacaaca aaagcgtatt
    13921 taaaatatca atgatcaaaa gaatggctaa cgctggcttt ggctttttaa tatacaaaga
    13981 aaggccaaaa aacaataagg tagtcgcaat cgggttgatc cacaagataa agtactggat
    14041 cgtaccttcc acacctaatg aaaaatctgc atagtaggca atgatcgtct tcagccagaa
    14101 aaagaaaaca gctgtcaaaa cgattgctaa acgattttgg cacaattttt taagttgtgt
    14161 cacgtatatc acttctctct attcacattt tttcttattt tgctcctgaa aaatgaaaat
    14221 ttcctaaaaa agttgttatc ttaagatttt tttattcttt ctaaagatta ttccattcat
    14281 ttttcacagc ataaaatcgt ttcggtgtaa gcggttaaaa tagtaaggag ataaaaatct
    14341 tgtggtgacc gatttactct atagtattca aaagtagttt ctcggtattc gtacaaaaaa
    14401 aacatactaa gattagaggg gaacatcatt gacgaatgat gttctcctct attttctatt
    14461 tttagattat agtgaagagt gaaagaaagt tcgaacaaag ccttgaggac tacgagtccc
    14521 tatcaaaaac cgaacttctt tactgtcatt ttagaatcag gtttacgtat gttggggcca
    14581 tgagcccgtg tataggaaat tggaatcgaa atgaagcagt aaagttcttt caacacttaa
    14641 aaaggaggaa aacaaatgcc ttacattctt gcattagatg tttcaatggg acacagctac
    14701 agtgtcctat acaaagacga cacctgctta tttgaagacg agatcgaaca cactcaaaga
    14761 ggatttcatg cattactcga agagatccag cacttgcctg aacgtccatt gattgttttt
    14821 gaagcaacgg gtatttattc cagaccagtc gaaaagttct ttcaagatca tcacttttct
    14881 tactgtttgt taaacccgtt agaagcgaaa aaacagatgg aagaaaacac cttacgcagt
    14941 tggaaaacag ataaatcgga tgcccatcgg ctcgcacaga ctcatcttaa aaatcaacgt
    15001 caaccaaaag aagtacaaag caatttttac ttagagatgc gcgatttagc ccgtttttat
    15061 caagaaattg agaagaaaat cactcgactg cgcatggatt tacacaactg ccttcagttg
    15121 acttttccag agcttgaaca gttcttttca aacaggctga ccccttacgc attgacattg
    15181 atccgtcttt ttccccatcc agattttgta ttggcatcta ctcggacaaa aataaaaaat
    15241 aaactgatca acgaaacacg ggaaaaaatc tccgcgaatc gtgccgagca aaaggcagat
    15301 caaatcatcc actatgcaca gtgtgcctat ccagctgttg aaaaagacag tattcactgt
    15361 caaaaaacga tctattacgc cgaacttctc caagatttac tcgaacaaaa aaaagcactg
    15421 gcgacacaaa tgatcaaaaa agccgaagcc tctccttgct ttttgttgta ccaaacgttc
    15481 cctgggattg gcgcactcac agccgcttta ttgctcggtg aattaggaga tatcacacga
    15541 tttaaaaccc ataaacaatt gaatgccttt gtcggaatcg acatccgacg gtatcattca
    15601 ggtaagtata ctggccaaga ccgcatcaat aagcgtggta atcccaaagc acggaaaatt
    15661 attttcttca ccattcgaaa tatgattcgc cagcaacgcg cagctcctaa tcatattgtc
    15721 gattattact acaaattaaa aaagcaacct atccctaaga aagataaggt tgccacagtt
    15781 gcttgtatga acaagcttct gaaatgtatg cacgctatgg ttagggcaca tacagagtac
    15841 gactatgcgt acgcggtctc agtggaccat taactaaacc acttacagta taccacttct
    15901 ttgcccagat cgaaaatttt ttacgaacgg ggctttattt agtgtgcctt gattttgtcg
    15961 ctttcgttta cgagctattt ttcttgcacc ttttacttga ctaatcgtag gaaagaggct
    16021 gtgacaaaat tcttgtcgca gctccctatt tcttgatgca ggacagcttt ttcacaacct
    16081 ctcca
//
LOCUS       DACECR010000067        15313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.067, whole genome shotgun sequence.
ACCESSION   DACECR010000067 DACECR010000000
VERSION     DACECR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.067"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            278..787
                     /locus_tag="IWO17_001933"
     CDS             278..787
                     /locus_tag="IWO17_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0290801.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IWO17_001934"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IWO17_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0290802.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IWO17_001935"
     CDS             2322..3302
                     /locus_tag="IWO17_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0290803.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IWO17_001936"
     CDS             3424..4842
                     /locus_tag="IWO17_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ0290804.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IWO17_001937"
     CDS             4913..6382
                     /locus_tag="IWO17_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0290805.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IWO17_001938"
     CDS             6392..6859
                     /locus_tag="IWO17_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0290806.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IWO17_001939"
     CDS             6895..7767
                     /locus_tag="IWO17_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0290807.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="IWO17_001940"
     CDS             7849..10383
                     /locus_tag="IWO17_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0290808.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="IWO17_001941"
     CDS             10539..11312
                     /locus_tag="IWO17_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0290809.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="IWO17_001942"
     CDS             11305..12096
                     /locus_tag="IWO17_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0290810.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="IWO17_001943"
     CDS             12106..12597
                     /locus_tag="IWO17_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0290811.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="IWO17_001944"
     CDS             12615..13025
                     /locus_tag="IWO17_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0290812.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="IWO17_001945"
     CDS             13029..14429
                     /locus_tag="IWO17_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ0290813.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="IWO17_001946"
     CDS             14452..14826
                     /locus_tag="IWO17_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0290814.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5315 a         2156 c         3060 g         4782 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaaaatg ctcactccta gagtaagcgc
    15241 cccatagaac agtaccttac taacaaatgc acctcaagat atacttgagc tatcaagaag
    15301 tctggaggtt ttc
//
LOCUS       DACECR010000068        15287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.068, whole genome shotgun sequence.
ACCESSION   DACECR010000068 DACECR010000000
VERSION     DACECR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.068"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="IWO17_001947"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="IWO17_001947"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="IWO17_001948"
     CDS             complement(580..1353)
                     /locus_tag="IWO17_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0290815.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="IWO17_001949"
     CDS             1497..1847
                     /locus_tag="IWO17_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0290816.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="IWO17_001950"
     CDS             1828..2544
                     /locus_tag="IWO17_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0290817.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="IWO17_001951"
     CDS             2544..3992
                     /locus_tag="IWO17_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0290818.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="IWO17_001952"
     CDS             complement(4062..4571)
                     /locus_tag="IWO17_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290819.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="IWO17_001953"
     CDS             complement(4561..5286)
                     /locus_tag="IWO17_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290820.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5509..7629)
                     /locus_tag="IWO17_001954"
     CDS             complement(5509..7629)
                     /locus_tag="IWO17_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0290821.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            7859..9037
                     /locus_tag="IWO17_001955"
     CDS             7859..9037
                     /locus_tag="IWO17_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ0290822.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9053..9811
                     /gene="aroD"
                     /locus_tag="IWO17_001956"
     CDS             9053..9811
                     /gene="aroD"
                     /locus_tag="IWO17_001956"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ0290823.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            9834..10319
                     /gene="ybaK"
                     /locus_tag="IWO17_001957"
     CDS             9834..10319
                     /gene="ybaK"
                     /locus_tag="IWO17_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ0290824.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10390..11643)
                     /locus_tag="IWO17_001958"
     CDS             complement(10390..11643)
                     /locus_tag="IWO17_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0290825.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11760..13109
                     /gene="gdhA"
                     /locus_tag="IWO17_001959"
     CDS             11760..13109
                     /gene="gdhA"
                     /locus_tag="IWO17_001959"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ0290826.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13221..14567
                     /locus_tag="IWO17_001960"
     CDS             13221..14567
                     /locus_tag="IWO17_001960"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ0290827.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(14875..15261)
                     /locus_tag="IWO17_001961"
     CDS             complement(14875..15261)
                     /locus_tag="IWO17_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0290828.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         4990 a         2737 c         3104 g         4456 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
     5521 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
     5581 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
     5641 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
     5701 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
     5761 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
     5821 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
     5881 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
     5941 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
     6001 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
     6061 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
     6121 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
     6181 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
     6241 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
     6301 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
     6361 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     6421 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     6481 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     6541 gaatgtacca gcgttcccat ggaataccgc actaatgtaa ccttgtgttt gacttaaaat
     6601 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     6661 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     6721 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     6781 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     6841 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     6901 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     6961 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     7021 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     7081 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     7141 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     7201 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     7261 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     7321 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     7381 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     7441 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     7501 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     7561 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     7621 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     7681 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     7741 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     7801 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     7861 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     7921 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     7981 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     8041 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     8101 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     8161 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     8221 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     8281 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     8341 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     8401 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     8461 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     8521 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     8581 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     8641 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     8701 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     8761 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     8821 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     8881 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     8941 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     9001 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     9061 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     9121 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     9181 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     9241 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     9301 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     9361 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     9421 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     9481 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     9541 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     9601 ggagttgact agacgggcaa atgctttttt ctccatgcct attattacga tgtccatggg
     9661 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     9721 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     9781 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     9841 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     9901 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     9961 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
    10021 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
    10081 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
    10141 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
    10201 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
    10261 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
    10321 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
    10381 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
    10441 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
    10501 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
    10561 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
    10621 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
    10681 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
    10741 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
    10801 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
    10861 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
    10921 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
    10981 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
    11041 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
    11101 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
    11161 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
    11221 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
    11281 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
    11341 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
    11401 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
    11461 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
    11521 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
    11581 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
    11641 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
    11701 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
    11761 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
    11821 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
    11881 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
    11941 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
    12001 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
    12061 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
    12121 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
    12181 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
    12241 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
    12301 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
    12361 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
    12421 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa
    12481 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
    12541 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
    12601 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
    12661 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
    12721 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
    12781 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
    12841 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
    12901 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
    12961 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
    13021 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
    13081 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
    13141 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
    13201 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
    13261 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
    13321 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
    13381 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
    13441 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
    13501 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
    13561 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
    13621 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
    13681 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
    13741 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
    13801 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
    13861 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
    13921 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
    13981 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
    14041 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
    14101 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
    14161 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
    14221 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
    14281 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
    14341 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
    14401 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
    14461 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
    14521 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
    14581 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
    14641 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
    14701 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
    14761 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
    14821 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
    14881 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
    14941 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
    15001 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
    15061 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
    15121 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
    15181 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
    15241 caaccctaaa atttttttca tgaaagatct ccttttttat ttttatt
//
LOCUS       DACECR010000069        15217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.069, whole genome shotgun sequence.
ACCESSION   DACECR010000069 DACECR010000000
VERSION     DACECR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.069"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(24..722)
                     /locus_tag="IWO17_001962"
     CDS             complement(24..722)
                     /locus_tag="IWO17_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ0290829.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="IWO17_001963"
     CDS             complement(715..2067)
                     /locus_tag="IWO17_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ0290830.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="IWO17_001964"
     CDS             complement(2290..3690)
                     /locus_tag="IWO17_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ0290831.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="IWO17_001965"
     CDS             3851..4588
                     /locus_tag="IWO17_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ0290832.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="IWO17_001966"
     CDS             4713..5192
                     /locus_tag="IWO17_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ0290833.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="IWO17_001967"
     CDS             5189..5956
                     /locus_tag="IWO17_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290834.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="IWO17_001968"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="IWO17_001968"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0290835.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="IWO17_001969"
     CDS             complement(6859..7461)
                     /locus_tag="IWO17_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0290836.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="IWO17_001970"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="IWO17_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="IWO17_001971"
     CDS             8142..8726
                     /locus_tag="IWO17_001971"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ0290837.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="IWO17_001972"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="IWO17_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ0290838.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="IWO17_001973"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="IWO17_001973"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ0290839.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="IWO17_001974"
     CDS             10627..10806
                     /locus_tag="IWO17_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290840.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="IWO17_001975"
     CDS             10828..11853
                     /locus_tag="IWO17_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ0290841.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="IWO17_001976"
     CDS             12028..13272
                     /locus_tag="IWO17_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ0290842.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="IWO17_001977"
     CDS             13425..14372
                     /locus_tag="IWO17_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0290843.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="IWO17_001978"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="IWO17_001978"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ0290844.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
BASE COUNT         5188 a         2720 c         3111 g         4198 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gcttttt
//
LOCUS       DACECR010000070        15202 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.070, whole genome shotgun sequence.
ACCESSION   DACECR010000070 DACECR010000000
VERSION     DACECR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.070"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..609
                     /locus_tag="IWO17_001979"
     CDS             4..609
                     /locus_tag="IWO17_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0290845.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            629..991
                     /locus_tag="IWO17_001980"
     CDS             629..991
                     /locus_tag="IWO17_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290846.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            994..1176
                     /locus_tag="IWO17_001981"
     CDS             994..1176
                     /locus_tag="IWO17_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290847.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            1193..4612
                     /locus_tag="IWO17_001982"
     CDS             1193..4612
                     /locus_tag="IWO17_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0290848.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            4663..5400
                     /locus_tag="IWO17_001983"
     CDS             4663..5400
                     /locus_tag="IWO17_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0290849.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            5410..7701
                     /locus_tag="IWO17_001984"
     CDS             5410..7701
                     /locus_tag="IWO17_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290850.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            7725..9806
                     /locus_tag="IWO17_001985"
     CDS             7725..9806
                     /locus_tag="IWO17_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290851.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLMANINADSFS
                     QGSGALSVVDDGDEVVVTLDPNNKLEIFKAKSQPALVVGKTYTMSVEIMLEGDFTGDP
                     SKIGLRYIKMPNWVSGLYTRNTLTATKGVWQKLTGTVKITAASDNAESWFIMLQNKDA
                     NNSLSGKLRLRHAKLEEGSTATPYQPNLLDAPYYLSKVALGENIADPAVSFPIKTSAY
                     RLYGVNMLEEFKVGQRYTITIKGTKPATQDFWAYNGGNISLERMTPVEGLVDVWTCSF
                     TILKLDSSSPSLLSIYQTPQSTVGSCQIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYG
                     SAYSFVITYSI"
     gene            9820..10167
                     /locus_tag="IWO17_001986"
     CDS             9820..10167
                     /locus_tag="IWO17_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290852.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            10160..10300
                     /locus_tag="IWO17_001987"
     CDS             10160..10300
                     /locus_tag="IWO17_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0290853.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            10400..10789
                     /locus_tag="IWO17_001988"
     CDS             10400..10789
                     /locus_tag="IWO17_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0290854.1"
                     /translation="MTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTGLAKAWKLKEI
                     SSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEGISFLENISVF
                     LPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            10786..11796
                     /locus_tag="IWO17_001989"
     CDS             10786..11796
                     /locus_tag="IWO17_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0290855.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     SDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPD
                     VNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIP
                     KR"
     gene            11986..12059
                     /locus_tag="IWO17_001990"
     tRNA            11986..12059
                     /locus_tag="IWO17_001990"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12020..12022,aa:Ile,seq:cat)
     gene            12736..13898
                     /locus_tag="IWO17_001991"
     CDS             join(12736..13012,13012..13898)
                     /locus_tag="IWO17_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0290856.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFKERDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGPKRQVIDANK
                     HKYSISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAK
                     RGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIV
                     TDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFH
                     TDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLA
                     QLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
     gene            complement(14028..14477)
                     /locus_tag="IWO17_001992"
                     /pseudo
     CDS             complement(14028..14477)
                     /locus_tag="IWO17_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14821..>15202
                     /locus_tag="IWO17_001993"
     CDS             14821..>15202
                     /locus_tag="IWO17_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290857.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIK"
BASE COUNT         5269 a         2417 c         3131 g         4385 t
ORIGIN      
        1 acaatggcta aaacaacaac agtaacaact tttgacaata taagtattaa aagaatttct
       61 tttaatttta agaatgcaac aaatgcaatt tcaactgatt gtaatggaca attagatggc
      121 gaaacagaaa tgcagagaat tgtaaaaaaa tgcggttcaa cagaagtaaa atcgaaatct
      181 aaaccaatca atatgacggt aacaattact gcacatgtac cgatggaagt ttatcgacgc
      241 ttcaatgggt tgaaacaaga tgaacgtatt aaatcgggaa tttactctta tggtcctgat
      301 tccgtagggg aagatttctc tcttgctgca gagatcgtgg atgatttcga agaaaaaaat
      361 aagctgattg gtatgttagc atgcacttcg aatacaggat taacattctc tattgaaaat
      421 ggtgcggatg aagtagctgc gttagaacta gaaacaaaag ttatgcaaga tgaatttggt
      481 aaattttatc atgaagcaat tgttgcagaa cttgaagaag acttaacaga tcaatggatg
      541 acgaatctat ctgctgatgt gattaaaaaa acttcagttg tgacaactac ggccactcaa
      601 tcacagtaaa aaaaacggag gtagcgaaat gaacgaagat tactcaaaaa ttgaactaaa
      661 cgatggaaca attttgaatt tagaacctaa actgaatatc aagaaattat tgatgatcaa
      721 tagagatttt aacacagacg agtttgcaaa aatgactgtg ggaaaaggat ccatggatat
      781 ttctgttatt caaggtgcaa aggctgtgta tattgcttac cgccaagcga acatgactga
      841 ttatatttca ttcgatgaat ttatcgataa atgggatttt gatatggcta ctgccagcta
      901 tatttatcaa ttgatgatgt tcaaacaagc acgcgatgct tatcaaaaag aatttgaaaa
      961 agcaaataag gaaaaaaagc ttcaaaagta aaaatgccaa agctcttagt tgaaacgtgg
     1021 gtcgatgtct attcgatgtt gaccgacgtt ttttctatgc cttcagattt ggttttaagc
     1081 gatatctgtt tagatgacat tttgcaaatg gcttacaaca agagtgctta tgaaggatgg
     1141 aaaaactatg caataaacca atcccagaaa aactaaagaa aggaggtaaa aaatggctaa
     1201 aaagagaaca gaagcagaag taacattcat agctaacgat gacggattga aatctacgtt
     1261 aaaagaaatc agtgctgaat taactaaaaa tagagcagaa ttaaaactag aacaagctca
     1321 attacaacag actggttctg aatcagacaa gttaggaagt aaattatctt ctttagaaaa
     1381 gcagtatgaa ttacaaagtc aaaaagttga agtaactagc caacgtttag ccaatgccaa
     1441 aaaatattat ggagaaaatt ccaccgaagt tcagaaactt gagagagaac tgattaacca
     1501 acaaacagcg caacaacgtt tgtcaaacga aattgataaa acgagtaatg cactagctca
     1561 agcaaaaggc gaaatacaga cgtacgagtc tacaatgcaa cagttggata gtgaacaaaa
     1621 aaatgttcaa gctagtgctt ctctgattga atccgaatac aaaaaatggc aagcaactgc
     1681 tggtcaatca gcttctgaat ccgagaaatt agcgaaagcc caagaatatg tttctcaaca
     1741 atctgaaaat gcagagaaaa cgatagatat cctgagacga cagttagaag ctacacagtc
     1801 tgagtttggc gctacatcca cagaagcaat gcagatggag gcgaagctta atgatgctga
     1861 acgtgaattt gaagagttag gacaagctgc taaaaatgta gatacaacta acttggacga
     1921 tatcggaagc aaaatagata tgaataatct aatggaagct tctgacgttt taagcgacat
     1981 tggcgataag cttacagaat tagggaaaca agcagtggac tctgctaata gtgtaggtag
     2041 ttcccagagt aaaatacaag ctaattttgg tttgactaaa caagaggctg aagaattaac
     2101 gaatgtagcc agagacattt attataaagg ttttggagaa tcgttagatc agtccacaga
     2161 tgcattgatt ttggtaaagc gtaatttagg cgatttaaat aatcaagatt tacaaaatat
     2221 cacggaacaa gctatggtcc tagaaaacac catgggcgct gatatggatg aaacgttacg
     2281 tggtgtaaat ggcttaatgg tcaatttcgg cttgagcgct caagatgcaa tggatttaat
     2341 ggtttcgggt actcaaaacg gtttagataa aacgcacgaa ttaggcgaca atatggcaga
     2401 atatagccaa ttatggagtc aaatgggata ttcagctgat gaaacgttcg gaatgcttca
     2461 aaatggttta gatgcgggtg cttataacct tgataaagtc aatgacttag ttaaggaaat
     2521 gggaatatcg ttaacagatg gtcgatttga gcaaaacatg gatatgttta gtgaaagtac
     2581 tagaaaagct tttgaagagt ggaaaaatgg cggaggaaca caaaaagacg ttattaattc
     2641 catgattcaa gattttagca atatggatgg tcaatacgac caattaaata aagcttctac
     2701 aatttggtct gcacttggcg aagataacgc gatgaaagtt gtccaatctt tgactgatgt
     2761 taaccataca tttgatgatg ttagtggatc tgcacaaaaa atgaatgaag attctactac
     2821 tccgttacaa gaattaaacg gaaaaatagc tgaattaaag gattcattag ctcctatagg
     2881 caacacaatc atagatgcac tcgaaccagt aattgatttt ctaggaaaga tggctgatgc
     2941 gtttaataat cttccacaac cagtacagga ttatgccgta gcgattggcg gattgactgc
     3001 tgcatttact ttattaatgc caataatagt tggcttcatg gctctaggtg gtcctactac
     3061 attaataata ggagcagtta ttactgttat tgctggagtt atagcaatta taaaaaactg
     3121 gggcgcaatt actgactggt ttaagggaat atggagtaaa ttcactgatt ggttgggtgg
     3181 tacttgggaa agtataaaag aaggtgcctc atcagtttgg gatggagtta aagaaacctg
     3241 gtctggattt gtagattggg ttcaagatat ttggcaagga gtttctgatt ggtttggaga
     3301 gctatggagc ggattagttg aaggagcttc caacatctgg caaggagtcc aagagacttg
     3361 gcaagcattc gttgattggg tttcaaatat ttggaacgga gtcaaagaag tatggtcgat
     3421 tatttgggca gacattgtag gaattgttca aataccatgg accttaataa cgtcattgat
     3481 tcaagccggt attaatatta tcgtgggtat ttttgatgta gctggacagt tattaggcgc
     3541 agcttggcaa gctgtttgga cacctatttc tgatttcctt aaaaacactt gggatactat
     3601 gacacaatgg gtaagcatcg cttggaatgg aattgtaact acattccata ctatatttga
     3661 tccagtagtg gcatggtgga atggtatatg gacagctatt agtactacgg cttcaaatat
     3721 ttggaattca attagtgcaa cagcttctag tatttggaac agtatcaaga atacaatcac
     3781 tagcttggta caagcagctg ctacagtaat tcaaaatatt tggtcaactg tatctagttg
     3841 gttaggtgga atttggaatt caatcagctc tacagcatca aatatctgga atagcgtgac
     3901 tagtagtata agcaatgcta taaacgcagc taaaagtgcc attcaaagtg tttggaatag
     3961 tatatcttcg tggatcagcg gaatttggaa cggtatcaaa aacactgctt tgaatctttg
     4021 gaatggaatt acaagcacta ttagctctaa agtaaacgat ggaaaaaatg caatttcaag
     4081 cggttggtcc aatctaacag gtattgtttc cgacatattc aataatgtta aaagtacaat
     4141 tgctaacatt tgggaaggta tcaaaaagac tgttagcgct ccaattgatt ggatcagaga
     4201 taaaatcagt agtatctttg ataatttgaa tatttctata ccacatattc cgttaccaca
     4261 ttttaaattg agcggagaat tcaatccatt gaaggggaaa atcccaacgt tgggtgttga
     4321 ttggtatgcg aaaggtagtg tgtttaattc tccgaatatt atcggtgtcg gcgaagcagg
     4381 acctgaagca gttttacctt tgaaaagatc tgtgctgcaa gaaattggtg atcgtatctt
     4441 gagtagcaca tcagtttcat ctagggcaca aacgattcaa cctgtgaata actacgaatt
     4501 caatttcaca attgatggta acgcagatga ggttactatg aagcaaacaa ctcaacaaat
     4561 cattgatagc attacaaaag ttcaaaatga taatgcttcg gcatggcgtt aaacaggaga
     4621 gtatttctcc tgttttttta gtattaaaaa ggatgtgaaa aaatgactga ttgtatacat
     4681 tctataatcg atggatttcc tgattatttg cataaattgg ctttagctga aagaccaacc
     4741 ataccttctc caaaaagaca gagagttgaa acttctgttt taggaaggtt aggtggctta
     4801 gtacaagatt actcgtttga agacatgtcg tttacattgc actataacta tttagaggat
     4861 gtggaagacc atcaagcgtt caagcaatcg ttttatatca tgcgtcattg gttaaattat
     4921 gcaaagaaat tagaattctc tgatgatccc aacgtctatt atgttatcca gactatcgat
     4981 attggggatg cagaaaacga tattgttgaa tggggagagt tcgatgtaaa tattactgcg
     5041 aaaccattcg caagagttca agaagatgta cctataaccg tagataaacc acagtcattt
     5101 aacttgctga ataatagttt agaagaaagt tttccaaaga ttatcatcac tccttcagct
     5161 acttcatgcc agttcatctt aaatgattat gtgtttagtt ttgaaggctt agtagtagga
     5221 actgacgtag tcattgatag tgatttgatg ctttgctacg aagagcaatc ggacggagat
     5281 attttagatc ggtccaacaa aatgaagacc atgcaatatc cgacattgca agtggatatt
     5341 aattatttta attgtactgg tttgagcaaa atacaaattt atcgtaatgg gttaaggtag
     5401 gtgaaataga tgatcgataa tttaataact atttacgata aaaacgacgc gaataattta
     5461 gctgaacatt tatatgatac gcaaggttta ggcgctttgt cagactggtt aacagctact
     5521 gttagcaata aattaaacgg agccgagata tttcagggta cttatccaat aagcggaact
     5581 aatgcagatt tgattgtaga aggacgtatt attcagtgtt atgtagatga aaatcgagca
     5641 aaacagcgtc tacggattta ttatgcaaag acttctgtaa taggcaatac gatagaagta
     5701 aaagctgaac ctattttcaa tgatataaga aaatcggtgt tgaataaata tgacagtgga
     5761 acagaaaaga tcactgctag tcaggcatgg caaaacgcaa aaactttagc gaaaccagtt
     5821 attccttcac agttttcttt ctcgtcatta gtagatacgc ttgctaatgt gaagatagaa
     5881 aaggcgaatt ttttagaatt ctttggtgga aaagagggat ctattctaga tcgatttcat
     5941 ggggaatttc taaaagataa taacacatta cgtcatgaaa aaaggctagg cacggatcat
     6001 aaaatcaaag cgatttatac taaaaactta actggtcttg acttagagat tgatgctcaa
     6061 agtgttttag ttggagttta tccattcatt agcagctctt cagaaggaga agacgagatc
     6121 actctaccag aagaagttat tttcacggat tacgtggatg attatcctgc tggatatgtt
     6181 tcttttgttg attttaaaga caaagcgact gatgtagcca cattaaggga agctgctaaa
     6241 gactggttga aaacaaacat agataaacaa aaaccacaag tgagtggttc gattgaatta
     6301 gtaccattga ggcatcaaag aggctatgaa aaatttgttg atctagaaaa agtttcgatg
     6361 ggtgacggag tagatgtgta tcatccacag ttaaaagtga atatgtcagc gagaattgtg
     6421 gaatatacgt ttaatgttct aaccaattca tacgataaat tagttgtagg aaacgtcaaa
     6481 acaaacttct tagaaaacac agagaataat gtcagcaatt tgattaatga tgccattgat
     6541 caattgaaaa acggtggcga aatcagtgat ttactcaatg atattgtaga tcatcaaact
     6601 gatatgatta ctggtcaaaa tggtggttat gttttattag atcctaaaga agcgcctagt
     6661 cgtattttga ttatggacac accagataag aataccgcaa ggaatgtttt acaaatcaac
     6721 aatgctggta ttggtttttc taaaactggc attaatggaa catatgaaac ggcatggacg
     6781 ttagatggcg gattcaatgc ctcgtttatt acggctggtg aaatagtagg aattactatt
     6841 agaggtacta cattaattag tgatggtgct gattatagaa caagtattgc taatggcaaa
     6901 atgacttggt actcaaaaaa agttaacaaa gatattatgg agctagaagc acgtgattat
     6961 gtaagtgctg atgccggtat tgtatcatac accatgaaaa ctggtggtgg tttcatgatt
     7021 agaaatccac agggtaactt ggtttttagt acgtgggata atggtaataa cagaccgttt
     7081 ctatcttttg gtgcgcccaa tttcaggtat agcaatgcta gttatgtaac ttctggcgac
     7141 ggtagttctt taagcattaa tggtagtgcg ggtaactcat gggaatttaa ggtagctggt
     7201 aggactatga aatttactag tgatggtatg ctaacgttac caggttgttt ttttggttca
     7261 tgggaagatg ggaaacttgc taggtttgaa caatcaacgg tacaagtata taaagatttt
     7321 actgttagag gtactaaaaa ctcaactgta ccaacagaac attatggaca acgactattg
     7381 aacgcttatg aaactccaga atattatttc gctgattatg gggaagccgt tacaggtgac
     7441 aatggtaaag ttcgtgttga tattgacccc atgtttgctg aaacagtaaa tctaagtcgg
     7501 tatatgacac atgtgacacc tacagaacta gttttgtgtg ctgttactca tgaagatatt
     7561 gaccatttca tcattgaaac tagtaagcca aacgtattag ttagatggaa tttagtggca
     7621 caccgtctag ggtatgaaga tattagatta aaagaggata cagcatatga tagcacagtg
     7681 cttgaccaaa aacgttttta aaacgaagac aaggaggtat ataaatggct agcagtttat
     7741 ataatttggc tttagatttc agcaaagaat taaactacac caaagctatt atggctcgtc
     7801 aaggtgataa agggattacg gtgacggtta aaccgtttct aaatggcttg cagatggata
     7861 cgagtggcgg aacatttact ttaaaaggaa caacaccatc taaccgttac gtagataatg
     7921 ttgcaactag tgtaactagt gaagaagtca cgttttctct tgatggcaca tttatgagtg
     7981 aagcaggata ttataaacac tgctacgtag aatatagaaa agacaatcaa attttaacaa
     8041 cgcaagatat catttttttc tcactaggag tgtctgacat ttcgcaaggc caagccgatg
     8101 aatatgtttc gcaattagaa gagttgattc gaaagtacaa cgaaactttt gatgctttta
     8161 tggctgaaat caaaggtaga gtggatagct taaatcaaca gattactgat ttaactggtc
     8221 aagctaaaac gctacaagac aagttagatg ctctgaaaga agaaatttct aagttaggta
     8281 acttacaagt gatgtacagt aacagcatcg acttcggggg ctacgattat agcgggaatc
     8341 cgaatttaat ggctaatata aacgctgaca gcttctcgca aggtagtggt gccttatctg
     8401 ttgtagatga tggtgacgag gtagtagtca cgcttgaccc aaacaataaa ttagagatat
     8461 tcaaagcgaa aagtcaacca gctctggtag taggaaaaac atataccatg agcgtagaaa
     8521 ttatgctaga aggcgatttc actggagacc ctagcaagat aggcctaaga tatattaaaa
     8581 tgcctaactg ggtatcaggg ctatatacgc gtaatacatt aactgctact aagggtgtat
     8641 ggcaaaaact aactggcacc gttaaaatta ctgctgcgag tgataacgct gaaagctggt
     8701 ttataatgct acaaaataaa gacgctaata acagcctatc cggcaaacta cgtttgagac
     8761 acgctaaact cgaagaaggc tcaacagcca caccatatca accaaattta ctcgatgcgc
     8821 cgtattattt gagtaaggtg gctttgggtg agaatattgc tgatcctgca gttagcttcc
     8881 caattaaaac gagcgcatac cgtttatacg gtgtgaacat gctagaagag tttaaagttg
     8941 gtcaaagata tacaattaca ataaaaggaa ctaaaccagc aactcaagat ttttgggctt
     9001 acaacggtgg gaatatttca ttagagagaa tgactccagt agaagggtta gttgacgtgt
     9061 ggacttgttc atttactata ttaaaactag atagtagttc accaagtcta ttgagtatat
     9121 atcaaacacc acaatcaaca gtaggctcat gtcaaattga ctggctcaag atcgaaaaag
     9181 gcgacacacg aaccccgaat attgagcaat ataaataccg aggaatcggc atgcgagact
     9241 caaacaatcc aaaagattat gtttgggatc tagcaccaga atatgtcgaa gataatttgg
     9301 ccacagatat taaaatttct gaaattactg gtaaagcaaa caattatacc gatgggaaag
     9361 tatcggagat taattcgcag ttgactgctt caattaatga agtagacacc acagctaagg
     9421 atgctcaaac aaaagcgaat gctaatgcga ctgctataga tgaattagac aataagatcg
     9481 atgaacgcat taatgataca gctactacca cattaacagt tacaaacggg aataccggat
     9541 cagcaaagct ttatcgtgaa ggaaaaacag tttctatata ttttgtggct ttaaacggaa
     9601 aaagcagtgg tggaaatgat tcaacgatac taacaattcc agaaggctat cggccaccaa
     9661 ttagttttga gcaactggtt ggctcgatag accgttctac tttgaacagt gctcaactat
     9721 ctattggcgc agacggagcc attaaatgga gaagaaattc aagttatgga tcagcttatt
     9781 catttgttat cacttattca atttaaggga ggaaatctaa tgaaagtagt ttacaaatca
     9841 atcaagcctt acggattcga gcaaatcatt ttgaacaatc aagaaaatat ccctgaaaac
     9901 tgtacagaga ttaaaccacc agttcctaac tggagaccaa gatttgattt tgataaaaaa
     9961 cagtgggttg agttagctac tgaagaagag aagagtggca cagcggttga cgatattgaa
    10021 gatgtcgatc agttgaagca attaaatgct ctactgacaa aacaattggc gatatcggta
    10081 caggaacaag aaaaaatgca acaaatgtta gctcaattga cgatggaagt cgcaagtatt
    10141 aagaatggag ggaaaagtaa tgaatagttt tccgggcttt gaaaatataa aacaatttta
    10201 tgattgggga tgttatacgg accaagattt acttgactat gtaaacatga actgtttaac
    10261 aaaagaccaa tataagcaga ttactggaaa tgaaatttaa ttaagcacaa agttaaataa
    10321 aaagcgtact caaacgagtg cgcttctttg cataaaggag aaatagatat tggaaaaata
    10381 tttaaataca ctctcagtaa tgacaggggt gataggcggg acaattgttg gtttattggg
    10441 agggatggac aatatactac atgttttaat ttttcttgtg ggtgtggact tcttaactgg
    10501 acttgctaag gcatggaaat tgaaagaaat aagtagtgag ataggtttcg aaggattgtt
    10561 gaagaaagtc ttaatcttcg ttgtgattgc cgttgcggtt gacgtacaga aaatcgttgg
    10621 gaattctata cctctaagag aaatagttat tatgttctat gttgcaaatg aagggattag
    10681 ttttttagaa aacatttcgg tattccttcc tttgccagat aagttaaaag aggtattcca
    10741 acaaatacga aatgatactg aaaataaaga ccgaggagga accaaatgaa aaagaaaatc
    10801 tttgtaggag ctattatagc tcttttttta ttgccaataa acgcctttgc ttactcgatc
    10861 aacaatgaat tcaatttggg cgcaaacgaa ggtagttctc aagtagcaaa taaccaatac
    10921 atcctattgc atgaaacagc aaacgaaact gcgacaggac gaaatgaagc gcagtacatg
    10981 aaacgttctt ggtacaatgc ctatacagcg tatattgttg gcgatggtgg gatcgtttac
    11041 caagttggac aacctggtta tgtacaatat ggcgctggtt catatgctaa tgcaaatagt
    11101 cctgtgcaaa tcgaactgca acatacgcac gataaagtaa cgtttgaaaa gaactacaag
    11161 gcatatgttg aattagcgag agattccgct aataaatacg gtattccact tacattagac
    11221 acaccgtata atcaaccagg aatcaaatca catttatggg taactcaaaa catttggggc
    11281 gatcacactg atccgtatgg atacttatca gaaatgggtg taagtaagga aaaactggct
    11341 tacgatttag ctcatggatt tacagacgaa aacccaacga cttctgagaa caagcctgtc
    11401 attgatccaa cccgagcagg tgcagcaaat ccaactttat cagatggaac gaaccattct
    11461 cacattgatc agtttggaga aatcgaaaat gcgaacttac acgtcgctgg atggcacatc
    11521 gctaactata aatacgagta tattttcatt atggactaca atactgggaa agaattagct
    11581 cgagtaagag ctgatggaat ttatagaccg gatgtaaatc aagcttataa tacttctgga
    11641 aatgttggtt atcatgtatc tttcaatatg cgtaattttc ctagtaagaa agtctatgta
    11701 atgatgcggg caacgaatga tccagaggga aacactaaag gcggtgcgca agatttccat
    11761 gacaaacgtt ggtatttaaa tattcctaaa cgataaaaat agctcctcgt tgaggagcag
    11821 tacataacta tattgacaac tataaaaatt attcgataaa atagtgatgt tatcgcatat
    11881 cttcactatc acccataaat agtcacactc caagctatgc gataacaggt ttgttgccac
    11941 acattctact ggttgattgt ttatggcttt atgtggcaac aaccagtacc cttagctcag
    12001 ttggtcagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta cagggtacat
    12061 taacgtagcc atttgaatcg ttctgtgtta gaattttttt gaagagtatt atacaagcta
    12121 aagcttttct tcattgccac tcaaatgagt ggctttttta tgtatccttt tatggattaa
    12181 tgaaaggatg tttcacatag ttatacttct gtatatttga aaagttttac tttgattttt
    12241 aaatagaaag acatttgggt taaattgtga gataataata aagaagagtt taaagcgcac
    12301 cccaaaccac ttccccataa gtgtgttacg ctttaaactc ttttatattt gaagccatta
    12361 aaaagcatac catatttttg aaaaaaagtg agaaaaaagg cttacaattg gagtggtagt
    12421 taattagtga cttatttttg attttatagc actgatacta taaaatatag atatcatcat
    12481 attacacaat cttaatacta acttaaaaaa tatctccttt cacaagtatg gtgataaaat
    12541 tcgttccggg ctaccttttt aggtagccta ctttaatctt tatacctttc tggatcaacg
    12601 aaagtatact ttatatagtc ataacgccga tgatcgctac gtgcgtccgg cacgtcagtc
    12661 atgaatcggc tacactagac tagacagaaa aaataaggtg tgtagaataa taaaaaacac
    12721 actggaggat ttttcatgtc aagacgtcaa cgaagaacct attcaaaaga attcaaacaa
    12781 caaatcgtcg atctctatct cgctggtaag cctcgcgcag aaattattcg agagtatgag
    12841 cttacgcctt cttctttcga taaatggatg aagcaagcac aatcaacggg ctcattcaaa
    12901 gaaagagaca acttaacacc agaacaagca gaattgatcg cactaagaaa gaaaaataag
    12961 caactcgaaa tggagaatga tattttaaag caagcggcgc tgatattcgg acgaaaagac
    13021 aagtaattga tgccaacaag cataaatatt ccatatcagc gatgtgcaaa attctaaata
    13081 tttctcgtca aacctactat tatcaagcga aaccgatcga aaatgagtcc gacttagaag
    13141 agatcgttca ggaagagttt attcgaaacc gaaaggctta cggtacccga aaattgaaga
    13201 agtgtttagc aaagcgtggg cttcaactca gtcggcgccg aatcggtcga atcatgaaac
    13261 gccgcggatt gacatctacc tatacgatcg ctcattttaa agggcaacga acagcttgta
    13321 atgaagcgaa aacagcgaat gtattagatc ggacctttac acaagaacag ccattggaag
    13381 ccatcgttac ggatcttact tatgttcgcg tggggaaaaa gtggcattat atctgcttaa
    13441 tacttgattt gtttaatcga gaaattattg gttattcctg tggtgagaag aaagatgcct
    13501 cattggtaaa agaagccttt ggacggatac cgtattcttt aacagacgtc aagctttttc
    13561 atacagaccg gggaaaggaa tttgataacc aaaccattca tgagattctg aatggttttg
    13621 gaattactcg ttcattgagt aggaagggtt gtccgtatga taatgccgtt gtggaatcaa
    13681 cctataaatc tgtcaaagta gaattcgtgc atcaatacca atttgagaca ctggcacagc
    13741 tacgtctaga attgtttgat tatgtgcatt ggtggaacta tctacgctta catggcacgt
    13801 tggcgtatga aacaccgatc caaattcgac aacagagatt ggcgaagcga atccttgata
    13861 atgagcgcgg atctgatacc tctggagagg cagcgtaatt gaatgattgt gcttctgccg
    13921 gagaaaatca gatccgagga tgctcattgt caagggcaat cgtagccata acaccgcagc
    13981 attcacaaca ccttataatt tttgtcaaaa aaagtgttgc cattccactt ttttaaatat
    14041 gcgttctctg ctcttaaacg aataacttct tcttcaagag actcatcttt aagtttttta
    14101 ggaatgttta gcttggaatt catactaatt tttctgcccc ttttttggct ctcaagtgaa
    14161 gaagcaccgc cttcttcata ttgctctatc catttactta gagttctatt tgaaccgata
    14221 ttaaattttt tagcagtttc ttggatagaa agaccatttg tttccatata ttctataaca
    14281 tcaagtttaa attttgtagt gtagcttttg ccacctccaa ccaagccttc ccaaccatga
    14341 tagttataaa tccttaccca atgtctaacc agtgtacgat ttatttgata tttatgtgca
    14401 agatatttgt agccgccttc gttatttaaa tagtctgaaa ctactttttt cttaaaaaca
    14461 aatgtatatt tccgcaaaaa aagcacccct tttaattaga tttctagtct aacttttggg
    14521 gtgcacatca tttttgaggg gaagtacata tagttgttga tatagttgct aatattgtta
    14581 gctaatttgt tagtaaaact gtatacgaat ttagatagta aaaaaacagt ttgttcctta
    14641 tatagaggca cttttactac tgattaaatg tcagatatgc ttacaaatat acttataaat
    14701 atgcttacaa aattagctaa tatttccgtt gacaaagctt tgttactatt gttaaactaa
    14761 attgactagc aaaagtgaaa cataaattta ttaaataaat gtaaaaatta ggagaaatca
    14821 atgtcttata gagtacaatt aataatttca gaagatgtag aaagtcagca atttggcact
    14881 aatgttatta ataaagtaat taatccagct ctttctatta atgcgccttt aattcctact
    14941 gctttatcat tttctgtaac agctgtggtg tctgagatag aggatacaga aaagataaaa
    15001 atagtagaaa ttgaagtttt aaacaagaat gaaaaacaaa ttttttctac gggtgaagta
    15061 tcggtaagct tgcctcctca agttaacgat ataaacttta atataaacgc cagaaatgtc
    15121 ttggttgaag aagcaggaga gcattatgct gttgttaaat ttaatggaac tgagattgga
    15181 aggcaaatct ttgatatcaa gg
//
LOCUS       DACECR010000071        14943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.071, whole genome shotgun sequence.
ACCESSION   DACECR010000071 DACECR010000000
VERSION     DACECR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.071"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(9..530)
                     /locus_tag="IWO17_001994"
     CDS             complement(9..530)
                     /locus_tag="IWO17_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0290858.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..4421
                     /gene="nifJ"
                     /locus_tag="IWO17_001995"
     CDS             729..4421
                     /gene="nifJ"
                     /locus_tag="IWO17_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ0290859.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4610..5497
                     /locus_tag="IWO17_001996"
     CDS             4610..5497
                     /locus_tag="IWO17_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ0290860.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTRAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5494..6663
                     /locus_tag="IWO17_001997"
     CDS             5494..6663
                     /locus_tag="IWO17_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290861.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6687..8042
                     /locus_tag="IWO17_001998"
     CDS             6687..8042
                     /locus_tag="IWO17_001998"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ0290862.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="IWO17_001999"
     CDS             complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="IWO17_001999"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ0290863.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10329..10619
                     /locus_tag="IWO17_002000"
     CDS             10329..10619
                     /locus_tag="IWO17_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290864.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10632..11657
                     /gene="sppA"
                     /locus_tag="IWO17_002001"
     CDS             10632..11657
                     /gene="sppA"
                     /locus_tag="IWO17_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0290865.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11660..12319
                     /locus_tag="IWO17_002002"
     CDS             11660..12319
                     /locus_tag="IWO17_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0290866.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12476..13408
                     /locus_tag="IWO17_002003"
     CDS             12476..13408
                     /locus_tag="IWO17_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290867.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13562..14851
                     /locus_tag="IWO17_002004"
     CDS             13562..14851
                     /locus_tag="IWO17_002004"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ0290868.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
BASE COUNT         4862 a         2683 c         3286 g         4112 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
     4321 cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta
     4381 aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat
     4441 aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt
     4501 tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca tatcagcctc taaaattaaa
     4561 acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca
     4621 aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc
     4681 tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact
     4741 gatccaactg ctgcgaggca caagagcgat tcagcttctg aaaggtgtag ctatttttat
     4801 tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca
     4861 agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg
     4921 actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga
     4981 tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg
     5041 tgcgttgatc actatcgaac gtcatactgg attagatgag tatattgaga cagggattgc
     5101 tttagatgca gatatcactg gggagctgct gatcaatatc tttattccta atacaccgct
     5161 gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc
     5221 tctatcagaa agtatgttga tccctaaaga attcgggact cgacaccggg cagctgtcgg
     5281 tataagcgaa gtcagtgacg cgattaccat cgttgtctct gaagaaaccg gggacgtcag
     5341 catcacgtta gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatctt
     5401 gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt
     5461 cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc
     5521 attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc
     5581 cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt tacgggggtg
     5641 ccgatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg
     5701 actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca
     5761 cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaagttccg
     5821 ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca
     5881 gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg
     5941 ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc
     6001 actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg
     6061 atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg
     6121 gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag
     6181 ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa
     6241 agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt
     6301 gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca
     6361 actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt
     6421 gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg
     6481 acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca
     6541 tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca
     6601 gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat
     6661 taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg
     6721 gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt
     6781 atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata
     6841 caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta
     6901 tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc
     6961 aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa
     7021 tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag
     7081 cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg
     7141 tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg
     7201 gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg
     7261 ttaatcgttt gtttgccgat cttgaaacag atttttatac aatgggcaca aatccagacg
     7321 ggttaaatat caatgacggc gtaggctcta cccatccaga acgtttagct gagatggtcg
     7381 ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg
     7441 tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact
     7501 tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag
     7561 gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc
     7621 gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc
     7681 acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga attcagttac
     7741 tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct
     7801 atccgcaaaa attagtgaat atccgagtaa gtgataaaaa cggtgcgatg gatgttccgg
     7861 caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag
     7921 ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga
     7981 aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt
     8041 aagagatatt ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt
     8101 ccttgttctt ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt
     8161 cgaatccccg taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta
     8221 gagtagttag ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc
     8281 cctctgagac aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc
     8341 gagtatgcgc accagtcact tcttcactga tgattccaat cactggtgtt ccttcttcga
     8401 tcaaagcaat cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac
     8461 tgatttcttt caatttcaaa gcagcttcaa gagaaacgtt gtagtcagcg ccacgtccaa
     8521 tgtaaaaggc atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt
     8581 tttgggcgat gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga
     8641 atacaagaga agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta
     8701 cagctatttg tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca
     8761 atagcagtgt aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa
     8821 gtgaagggta gccgagacgg ttgattttta ccagaacttg gcggctatct gctgtttcgc
     8881 cactttgact caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa
     8941 attcactggc taaatgaacc tcaacaggga tctgtgtcaa tttttccaga gtctgtttac
     9001 ctgctaaccc cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag
     9061 acaatgtatc gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt
     9121 gagcgatacg acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat
     9181 aggtaccttt ctcgatatct gaagaatcta cttgtgcttc gaatgaagaa cgttctacga
     9241 tgtcacctgc aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat
     9301 cttcaatctc aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca
     9361 cattgaatcc atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa
     9421 catcaggtgc tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag
     9481 cttttaaaaa cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga
     9541 taatctcagt atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt
     9601 cttcaaagtt ttcgattacg ccattgtgaa cgagcacgaa gcgtccactt tgggaagtat
     9661 gtggatgggc gttttcttca gaaggttgcc cgtgagttgc ccagcgtgta tgaccaatac
     9721 ctatatttcc tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg
     9781 ctttgaccag ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac
     9841 ggtattctaa tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca
     9901 ttccaacaat tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct
     9961 tccagctgca ggaaaatcct tttgttttat ctttgcagat attttttgtg gattttctgt
    10021 agagtgcagt ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg
    10081 tttacacaga cctggcgcta tcctaaaagg tataactatc ctcctctttt gttttttatc
    10141 tggaaacatt taaaatcata accttttgcg aatgttcttg tcaacaaaaa aagaataaaa
    10201 aataaattgg tatatatcaa atgctttgtc aaatgctctt tttatgtcaa aaaataatgg
    10261 gtttttatct atcatctttt tcaggagttc ggtacaatag aaagtaatcg gatagaggag
    10321 gagtaaaaat ggattcaaaa attgagatca tgacactggg aatgttaaaa aaacaattgt
    10381 ccaaattcga agcatcagct ggtgtatcag atgatacgaa aatattttta gatactggct
    10441 gggatagcat ccaagaaatc gcacctgatg ccttggaagt tgctgaagct cgtgagttta
    10501 cggtagaaga tgaattgaca aaagagtcgt tttccgggta tgcgcgggaa gaaaaagcag
    10561 agcggttcga cacttctgag cagtcagaaa ctgtgatcgt cataaaaaac ttgtattaga
    10621 aaggagaaag aatgaataag agacgttggg cagcagttct gattgctgca ggattatttg
    10681 tggtttcgtt agtttcagct agtttgacta agcctcagga aaacgatcaa attgagggat
    10741 tgaacagttg gttatatggg gatgaagaac tagcttcaaa tgtgttggaa gagggagaca
    10801 gtacgcaaaa aatcgtaaaa ttatcagtag atggaacgat tgctgcggga gggtcttcag
    10861 gtttatttgc ttctgaaggt tacaatcata aaaattttat ggaacaactg aaggcgattg
    10921 aagaagatcc aacagtcaaa ggtatctttt tagaagtaaa ttctcctggt ggcggtgtct
    10981 acgaaagtgc ggaaatcgct aagaaattgg atacgattcg taaagaacat gatatcccaa
    11041 tgtatgtcag catgaaaaac atggcagcta gcggtggcta ctatatttct gctcaagcag
    11101 ataaaatctt tgcaacagaa gaaacagtga ccggctcaat tggtgtgatc atgtcaggat
    11161 tgaattattc aggacttcta gaaaaactgg gtgtggaaga tacaacagtt aaaagcggag
    11221 cgttgaaaga tatgggttca ggaacgcgac cagagacaaa agaagaagaa gcggtgcttc
    11281 aagcatatat cgacagcgca tatagtcgtt ttgtagcaat cgtcagcgag ggacggaata
    11341 aatctgaaga agaagtcaaa aaaattgctg atggaaggat ttatgatggt gtccaagcaa
    11401 aagaagtagg tttagtcgat gaattgggat ttcctgaaga tgctttaaaa gctatgcgaa
    11461 aggaacaaca gcttgaagat gcggaactag tagaatattc ttccacgcca tctgggtttg
    11521 ctagtacatg gttaggaagt aaactagcag aattccaggg attgaagcct tctgagacta
    11581 gtcagatctt atccgttctt gagaatctag ggacagcgga atcaccaaaa gcaatgtatt
    11641 attacggagg tgagtaagca tgtcagagct ttcaaatgaa aataaggaac aagaaaaaga
    11701 taagcaacag cagcctgctg taggacctga ctccaacttt tcccaaaagg tactgagctc
    11761 ttttgaaaga gagcctttga cgcccgaaga aatcaaaaag aaacaacgta aatggaaaaa
    11821 gtatacggac acaagaaatg aaccgccaaa agcaaatgat tttcctaagt atttctatgc
    11881 aggttttttt atccgaatgt ttgctttctt ggtcgatctt ttgtgtatcg gagctattac
    11941 gcgtttaaca cttggtattg ccaaaaatct aggttggctt aatttttcag attcttatct
    12001 gagtatctac ggattcacag ccttacttat ttatctagcg tattttattt tattgacgaa
    12061 attcaatcat ggtcaaacga ttgggaaaat gatctttggt attcgagtca tcagctttga
    12121 tgaagaagaa cttagctggt caaccgtttt agttagagaa ggtgcttgcc ggttcattct
    12181 aaaatttcct ttgctgatgt tagggtatct tccagcaatc tttaatcaga aaaagcaaca
    12241 tctaggcgat tttttcacta atacaagtgt tgttacgatc aatctgatca aagcattcaa
    12301 ccaggaagtc aacagctgat gagcaataaa aagcagtaac agttgaaagc accttctaaa
    12361 agtcagatct aaaatctagc ttttagaggg tgcttcagtt atttttaatt gtcatgcttt
    12421 ttttgttttg ctactattga acatgagctg aacatgagca agaggaggac actgcgtgaa
    12481 gccgattgat tttccagaaa aatatgagaa tttaatgcga gtagcacaac aagcactagc
    12541 aaaccagcaa tatcagcagg caaaagaatt atttcaacga gcttacgagt tgaaagaaag
    12601 ctttgaggct aatagcctgc ttgttttttg tctttacgag ttggacgaga aaaaagaagc
    12661 attgaagcaa gcactcttgc atgaaaaaca gtatcttcaa aatgaggagt ttgctgaatt
    12721 ttattttgac ttgttaatca gtacacaaga ttttttatat gctagaaaat tgatagcaag
    12781 tacagatttt tatgaagctt tcgagcagcg ggtcattgaa aaaatccaat ttgcagaaga
    12841 attatccgga caaatggaga ggcagaagat caaggcactc cacaataaat cggaggaact
    12901 tccttcgttg gaacctgcac gtcagcttgc gtctattgat gagatcgaac agcttcctta
    12961 tcatgagttt atccaaacag cgagtaaact tattgtatcg ccagaggtcc atattttagc
    13021 aagggcaaaa ttactggaga cacttaggca attgaacgaa tgccatcctg ttttctattt
    13081 gacgattgaa gaaaagttag tcaaagtcat accaaaagat ctgccaaagc cgcagcagca
    13141 ggcaagttac cagcaattat gtgtattttc agatcgttac ggtaatgaag atgcgctgct
    13201 tagttccgtg ttaaatgaag aattcacgtt gcaatctgca attgtttatc ctgtctatga
    13261 tacatacatc gaagacccga agcgctggtt tcaactgact gtagaggcgt gtacaggaaa
    13321 aacgatgtat gaaggtacag aagatgaaaa acaagatttt ttaaaaaaaa gagaaaaaat
    13381 tttgcagcaa atgatcttct tccattagat tttatcagtg aaaaagataa aaatgttatg
    13441 aaaaacaagg attctatcca ttcaatatgt ttacaatttg aaagggttag tgtactattt
    13501 aaaggtatgc atgaaacgtt tttgatgtat aaaatgaatt tctaaactcg gagggaatta
    13561 aatgactgca acatttgaaa aaaaaggcac gaatgatggt gttttaaaat ttacgattgc
    13621 gcaagacgaa atccaaaaag gattaacaac tgcgtttaat aaagtgaaaa aaagcttgaa
    13681 cgtacctgga ttccgtaaag ggaaagtttc tcgtcaagta ttcaaccgta tgtacggtga
    13741 agaagcttta tatgaagatg cattgaatgc tgtattacca gaagcttatg aagctgctgt
    13801 caaagaagct ggcatcgatc ctgtttctca accaaaaatc gatgttgaaa gcatggaaaa
    13861 aggtcaagac tgggtaatca ctgctgaagt aacagtgaaa ccagaagtaa aattaggaca
    13921 atataaagat ctagttgttg aaaaacaaga ccgtgaagta acagatgctg acgtggatgc
    13981 acgtatcaaa cgtgaacaag aagcacaagc tgaattagtt atcaaagaag atgctgcagc
    14041 tgaaaatggc gatacagtag taatcgactt cgaaggcttt gtagatggcg aagcgtttga
    14101 tggcggaaaa ggcgaaaatt actcacttga attaggttca ggctcattca ttccaggttt
    14161 tgaagatcaa ttagttggtc ataaatcagg tgaaagtcta gatgtaaatg taactttccc
    14221 agaagattat caagcagaag atcttgctgg aaaagaagca gtcttcaaag tgacaatcca
    14281 tgaagtaaaa gcaaaagaag tgcctgaatt agatgacgaa ttcgcaaaag atgttgatga
    14341 cgaagtatca acattagacg aattgaaaga aaaatatcgc aaagaattag tagaaacaaa
    14401 agaaaaagca gcggacgatg caaaagatga agcagcgatc cgtatggctg ttgaaaacgc
    14461 agaaatcgtt gaattgccac acgtgatggt tcatgatgaa gtacatcgtt caatggacga
    14521 attcttgaac aacatgcaac gtcaaggtat ctctcctgaa atgtactatc aattaactgg
    14581 ttctacagaa gaagacttgc ataaacaatt cgaaggtgaa gcagaaatgc gcacaagaac
    14641 aaatcttgtg atcgaagcaa ttgcagctgc tgaaaatctt caagcaacag aagaagaaat
    14701 caatgcagaa atccaagaat tatcagaaac atacaacatg ccaatcgaac aaatcaaacg
    14761 cgtgttgaca gaagatatgc tggaacatga tgtgacaatg aaaaaagcaa tcgaattgat
    14821 cacaggtaca gcgacagaag cgaaagaata gaaatcaagt aataaatcat ttctgaagaa
    14881 gctacttcta gaaaattgtt tattcgaaat aagggactgt gacacgattt tgtcacagtt
    14941 cct
//
LOCUS       DACECR010000072        14477 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.072, whole genome shotgun sequence.
ACCESSION   DACECR010000072 DACECR010000000
VERSION     DACECR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.072"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(287..649)
                     /locus_tag="IWO17_002005"
     CDS             complement(287..649)
                     /locus_tag="IWO17_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290869.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IWO17_002006"
     CDS             complement(723..1103)
                     /locus_tag="IWO17_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ0290870.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IWO17_002007"
     CDS             1185..2360
                     /locus_tag="IWO17_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0290871.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2436..3020)
                     /locus_tag="IWO17_002008"
     CDS             complement(2436..3020)
                     /locus_tag="IWO17_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0290872.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3221..4219
                     /locus_tag="IWO17_002009"
     CDS             3221..4219
                     /locus_tag="IWO17_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0290873.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4331..4840
                     /locus_tag="IWO17_002010"
     CDS             4331..4840
                     /locus_tag="IWO17_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ0290874.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4870..5529)
                     /locus_tag="IWO17_002011"
     CDS             complement(4870..5529)
                     /locus_tag="IWO17_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ0290875.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5531..6295)
                     /locus_tag="IWO17_002012"
     CDS             complement(5531..6295)
                     /locus_tag="IWO17_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ0290876.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6303..6980)
                     /locus_tag="IWO17_002013"
     CDS             complement(6303..6980)
                     /locus_tag="IWO17_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ0290877.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7017..8402)
                     /locus_tag="IWO17_002014"
     CDS             complement(7017..8402)
                     /locus_tag="IWO17_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ0290878.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8612..8696)
                     /gene="ffs"
                     /locus_tag="IWO17_002015"
     ncRNA           complement(8612..8696)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWO17_002015"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8719..9234)
                     /gene="tadA"
                     /locus_tag="IWO17_002016"
     CDS             complement(8719..9234)
                     /gene="tadA"
                     /locus_tag="IWO17_002016"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ0290879.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9341..10198
                     /locus_tag="IWO17_002017"
     CDS             9341..10198
                     /locus_tag="IWO17_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ0290880.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10279..10614)
                     /locus_tag="IWO17_002018"
     CDS             complement(10279..10614)
                     /locus_tag="IWO17_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290881.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10681..12051)
                     /locus_tag="IWO17_002019"
     CDS             complement(10681..12051)
                     /locus_tag="IWO17_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ0290882.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12129..12665)
                     /locus_tag="IWO17_002020"
     CDS             complement(12129..12665)
                     /locus_tag="IWO17_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0290883.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12974..14455
                     /locus_tag="IWO17_002021"
     CDS             12974..14455
                     /locus_tag="IWO17_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0290884.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4459 a         2881 c         2354 g         4783 t
ORIGIN      
        1 tttttttaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atatttattc aaaataaaag gacgagacct tactaggttt
     2401 cgtccttttt cataattact ccgtaccata ccaaactatt ctgaaatttc aattgtttca
     2461 atcactacat catgtacagg tcggtcttgt ggaccacgtt gtacatttgc gatttcatct
     2521 actgtgtcca tcccttctag tacatgtcca aagactgtat gtcggaaatc tagccaagga
     2581 gttccgccac ctttatacgc ttcaatgatt tctgatggga atccagcacc ttctagttgt
     2641 ccaagcatat tagctggcac atttgtgtta tttacaataa agaattggct tccgtttgtg
     2701 tttggacctg aatttgccat agataaagct ccgcgaaggt tgaacagttc acgactgaat
     2761 tcatcttcaa aactttcgcc ataaatactt tcgccaccca ttcctgttcc agttggatcg
     2821 ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa tcacgccatc ataataacct
     2881 tttttggaaa gttcaatgaa attttgaacc gtcttcggtg ctagttcagg gaataattga
     2941 acagtaatgt caccacggtt tgttttgata acagcttttg gtcctttttc actttttaaa
     3001 tctagttgtg ggaatgccat attgtttcct cctaatattt gcatacttca taataataat
     3061 aacataatac ctaacctctc gtaaaaatac tagatttgtt ttcttttatt tttcatcact
     3121 agatacattc tataacttac cgtttcgatt tcgaattcaa acatttttga gaagcattct
     3181 caattaaatt ctgctatagt gtgaaaaggg aggaattttt atggaaatct atgacattac
     3241 aatcatcggt gcaggtcctg tagggatgtt tgccgctttt tacgcaggaa tgcgccaagc
     3301 taaaacaaaa atcattgaca gtctacctca acttggtggt caattagcaa ctttgtatcc
     3361 cgaaaaatat atttacgata ttcctgggta tccagcaata aaagcaagtg aattaatcga
     3421 tcaattagaa aaacaattaa ctacttttaa ccatacattc catttaaaag aagaagttct
     3481 ctcgcttact cgtgaagacg aagttatcga gattactaca aataaaggca tccattattc
     3541 caaagcagtc attttagctt taggaaatgg ttcattccag ccaagaaaat tgaatttaga
     3601 taatgctgaa tcatttgaaa accacggatt ggattatttc gtgaatgatt tgatgaagta
     3661 tgctggaaaa aaagtcgcta ttgctggagg tggtgattct gctatcgatt ggacgttgat
     3721 gttagagcct attgcaagcg aagtttactt gattcatcgt cgcccggaat ttcgtgcgca
     3781 cgaacatagt gtatcacgct tgaaatcttc atctgtcaac ttgcttactc cctatctcat
     3841 cgatggatta tcaggcaata atggggaact gacagatatc cggttaaaaa aagtgaaaag
     3901 tgacgagacg attgatttga tgattgattc tctgatcgtc aattatggat tctcttctaa
     3961 tttagaacat ctttcttctt ggggacttga tagtaccaga aatgccatta ctgtaaaatc
     4021 agatatgtct acttctattc caggcgttta tgcagctggt gacatctgta cctatgaagg
     4081 aaaagtgaaa ctgattgcta ctgggctcgg tgaagcacct actgcagtga acaacgcttt
     4141 acactatatt aatccaaagg aacgaactca gcctggtcac agcacaagct tatacgataa
     4201 aaatcttcgt cctagttgat tttttatatc tctttgcatt tcaagttaaa cccgaaagca
     4261 gtttcttgca tttttttcat gttccgttaa catgaaatag agaaagaaaa aataaagggg
     4321 gagcaataat atggtcgtca aaactgagac tttagaagaa aaggcaagag aactattgaa
     4381 ggaacgtggc gtaactgtag aagatatcgc acaacttgtc atgtttttac aaaaagacta
     4441 tatttctgat ttgactttgg cggattgtac tgaaagtgtc aacagcgtgc taacaaagcg
     4501 tgaagtacat aacgccatta tcactggaat ccaattagat atattagcag aaaagaaaaa
     4561 attgatgagt ccgttgcaac atattatcga agatgacgaa ggcctttacg gtatcgatga
     4621 gatcctagcc ttgtcaatcg tgaatgttta cggtacgatc ggctttacga attatggtta
     4681 tatcgacaag gtgaaacctg gtattttaaa agaactaaac agccacgatg gcgttcatgt
     4741 ccataccttc ttagatgata tcgtcggagc gatcgccgct gctgctgcca gtcgattagc
     4801 acacaaagaa ccagaaaaaa gaagtcgcct tactcaataa ttcaacaaaa aaagaaagaa
     4861 aacgtctatt tagacgagtt ctttcttttt tgtttgtttc aagctatttt ttcctaagaa
     4921 aagaaaaaca gcaaaaaata attctaataa tacaaaaaat aagataaagc tatgcatgaa
     4981 aaattcttct ggaagtacat tgatgattgg atcagtaacg ggatcgaata accagtcatc
     5041 gttgttgaaa aacgtttcat gaaaaaggat aaaaaagcga tcaaatccta tcatcatgaa
     5101 aaaaccgaaa acaactggca gtaacatgcc gatttgaaac ggacgaatca accgccaaag
     5161 ccgatcattc ttttgcaaat atttgataaa caaaatactc gggatcaaag tgatgaaaaa
     5221 cacagcataa tccactaaaa acaaaatttt cacttcataa aagtgatggg ctccgctagc
     5281 agaaactggg aaatctggca agctgaggat ggttttgaag ggattattga gataattcat
     5341 taggtgatca aaatttttca gtaacgtctc ttgatctaaa gaagtataat ccaaaatttg
     5401 caaatgtccg atatcaaata cgtatagtgg tcgaaagttg atcgtcacac caactgccag
     5461 actgatcagt gtcagaaata ggctgaacaa tccagcatat tccatccact gccatctcga
     5521 atttttcata ttagaagctc cattcatcca gtgattgttt cacataggta gctgggatag
     5581 gcaacccagg tacatcttct tcttttgtaa aacctgataa aacgagcaaa gtatctatat
     5641 tattgcgtat cccagcttgg atatctgttt catagttatc tccaaccatg attacttctt
     5701 ctttttgcag ccctaatact tcaacggctt tatccataat gatggcttct ggttttccga
     5761 tgtagatcgg cggcgtttgg gttgctgtct ctacaaaaga gatgactgac cctgctccag
     5821 gaagaaggcc gcgttcagta ggtatattct tatctgggtt cgttccaatg aaagtagcac
     5881 ctttttgaat agcaagtgtt gctttaacta ctttttcata ggtcagataa ttatctaagc
     5941 ctaccactac atagtcagga gcctcttctt cccatgtaaa tccggcagct aaaatcaagt
     6001 caatcaatcc tgcttctccg ataacataga cttttttccc ttttccatct gctttcataa
     6061 aatcgattgt tgccaaagta gctgtgtaga ctgtctctgg agctacatga atgtcaaact
     6121 catctgctag acgattagct actgtttctg gtgacttagt cgtgttattg gtaacaaaca
     6181 agaaaggtaa ttttcttttt tgcaattctt ccacaaatcg tttaccggcc ggtatgggtt
     6241 gtgtcccacg ataaatcgtt ccgtctaaat caattaaata tcctttatag tccattcatc
     6301 cactactctt ccctttttct gatcgtaaat gctgtttttg ttttttctac ttgtttaggt
     6361 tgttcttgtt tttttacttt ttcagaggtt tttgctgact ctgtttttcg atttttaata
     6421 accggttttt tgcgattaga tgcgactggt ccttttttct catcgatata cgcacgatta
     6481 tggttttttt tcttttttcg gcgctgagtt gtcttttctt ttttaccgcc gattctctcg
     6541 atgacaaaat acgcacaacc aaaattgcaa tattcgtaaa gatagtcttc caaggaatcg
     6601 attcgctgat ctggatgcgc ccgtcgatta tccgcttgga aaaatccttt taggcgtagt
     6661 tgttcataac cccaatctcc tacgatataa tcatatcggg ctagtacatc actgtaacgt
     6721 tctccaagct tttctgcatt aaatccttca cgataatcgg acacaagccg ataatgtctt
     6781 tcacctatca aaaaattact gtcatctaac agtgtcacta gctcgccttt gactttttct
     6841 gctttaggct cctcaacgat ttcttccaaa gcagctgtca gttcttcggt gactgttgtt
     6901 tcctgttcag ctgatgattt tttcttagca atttctgttt tatgctcaac gttctgctgt
     6961 tcattttgtt ttgcagacat gcgttcacca cctatctttt ttattatacc ttgtttttat
     7021 ttgattgggt agtgagcttt caagtaatct cctaccacac ttcggataaa ttcagggaac
     7081 aaaaattcat tttttccagc aatctcaatc gttggaaaaa atggaacaaa catcaaatga
     7141 tctacggtag taaaagtata atttttattc agatcgattg gcttgttctt ccaaaagact
     7201 tgatggttaa tagaatcata tgtgattcca ttatagacca tctgcccaaa aatcttaccg
     7261 cgaaatccca ttcccttcat aggatagtta cgtaaaaagt tacgattttt ttctatttct
     7321 aaaactaagc ggatcagatc atttccaccc aatgtgacat ttaacaagtg catcggatgc
     7381 ggcaacgcag tgtgaagctg atcctgattg acccgccctt ttggaatttc agttaaaaac
     7441 agaccactgt ttaatattgc tgcttccgtc tttcctcgtt ctttcaccgc ctcaaaggct
     7501 acttggataa acgaatgctc attgaagata tctagactca gagaaaatgg caaatctgct
     7561 acttcttttt cttttaataa ttcatgccca cgttctagat aactatttat ttcttgttca
     7621 tcttctgtaa aagctgtcat tgtttcggta gggatggctt tggctgaatg cttaaggatt
     7681 tttttctctt catcgatggt taaatggact tcaccgacat actgcccgta ctttccagcg
     7741 gctgctattt ggacatcatt gattacgtga ccttctctga ataaatgatg ggtgtgcgat
     7801 cctaaaataa catcaattgc tggtatttct tttgcaattt ggaaatcatc ctgaatcccg
     7861 agatgactca tcaaaactaa aacatccact tgtgaacgta aatcttcgac taattctggc
     7921 aaaatatcat aaggattccg tatgtcccag ccattcggat tataagtaag cggaaatgga
     7981 gcagtaaatg caattagtcc gattttcgtc tggtgtttcg ttgtaatgat tttatatggt
     8041 ttcgtccatg ccggtggctg gagcgtattt ttatcgaata agttatccag gatgatatca
     8101 aactcagcat gatcgtataa atgattcaac tcttctttgg aatttcctac gccttcattg
     8161 tttcctatcg tcgctgcatc ataaccaact tcattcatca gcaagatatt ttcctgtcca
     8221 ttcgttgctt ctgacaatgg atgccaacga tccacaaaat cacctagatc tactgcaatc
     8281 gttgtcgttt tttctttctt ttcgttttct cgtttacgtt gatccaaaaa ccttctgatc
     8341 cttggccaat tttctaaatg agaatgcaga tcattcgtat gtaaaatcac tattttttcc
     8401 atgtaccctc tcgcctctct ttatctttat cataccatag gcagcttttt cttccattga
     8461 aacaagctct atttcagagt ttttcctgcc ttgaagcaaa ttgtataagt acaatattcg
     8521 ataaatcagt ctggctaaaa aatcaggaac attcattttt acagtactat aaaaataaaa
     8581 aaaacagacc cgaaagtctg ttgtcgtttc attataatca gacacatggc ggaacctgct
     8641 tagtgctgct tccttccaga cctgacacgc ttcacaagcc caccattgtc ctagcctttc
     8701 ggcaaagaat agtatacctt atttttcatt cgttgaccag tcttttttta atttcttttt
     8761 tcgctggcgg agctttcgga aaaagtcagt caaaatcatc ccacattcgt cttctaaaat
     8821 gccaccttct acatatgctt ggtggttaaa ccgctcgtcc tccaaaagat tcatcaaggt
     8881 accggctgtt ccgccttttg gatcatacgc gccgaaataa acttctgcta cacgagacaa
     8941 aatcatcgcg ccgctgcaca taggacaagg ctctaaggta acaaaaagtg cagcatcttc
     9001 caagcgccag cttcccactt tttcacaggc ttccttgatt gcaagcattt ctgcatgtgt
     9061 agtagcatct tgcgtggttt ctctaaggtt atatcctctt ccaatcactt ttccatccaa
     9121 tacaaccact gctccaatcg gaacttcatg aagcatttct gctttctttg cttctgccaa
     9181 tgcaaggcgc atccattttt ctttttctac tgcatcaaga tttgtattta ccaattaccc
     9241 ttccctgcct ttgttttttc ttgttttact ttagcatatg ataaaaaggc tgtaactaac
     9301 ttttttttct atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg ataaattact
     9361 tgctctttac ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg atatcctttc
     9421 ttttgcaatt gatgatgctt ttatctggat caaacaagat tcattatctc aacaagaaac
     9481 cagccttttg aaagcgttat tccctgtaat caacgatcaa aaaaaacacc catggtatcg
     9541 ctatcttttt aaagatgctt cttgtgttca agaaaaaagc tttcgtttga tccagcttca
     9601 cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa gatactattt tggaattatt
     9661 tcgctattct gctgatttct ttttttatac agacacagat gcgctcttag tcgaacaaat
     9721 aaaccgtgat tatcttgatg gtactgaaat caacggtatt tttctatctt tagatgctga
     9781 ttttgatacg acaacgtctg tattcgttgg ttctttccat tcacctggca ccgacctcgt
     9841 ccctttgttt gctgaagaaa gaaaaatttt tttaacagag caaaacaatc tttatactca
     9901 cagccgtact ttttctatgc aagatgtggc tttacattac tatacaaaag atactgtaaa
     9961 agatagtaag ttgatcaaaa gttacataga actgatcaaa caaaatgaaa tggaagtgat
    10021 cattcaagaa ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa cactgtttct
    10081 tcatcgtaat actctaaaat atcgaatcga aaaatttcaa gaacagtctg gtttcaatct
    10141 aaaaaaagcc aacgatttac tgttttgcta tcttttattg cttcatgaac ataattaaag
    10201 atagattaaa ggggttgtga caataatttt gtcacaaccc cttatttcga atgaatagtg
    10261 ttcaaaagct agtttctctt acatcatttt ttcaacaaaa tgcattagcc ggtcttttac
    10321 atcttcggtt gaatctttga ttgaatcacc ataaacagag attttctttt ttcctgattt
    10381 gatttttgct aacattccca tcaaagaatc caagtcacta tctgaaagac catctacgat
    10441 ttctagtaat ttttcatttc catcaaattt gtcatagacg aattttttta ctttcgtccg
    10501 agtagtcata tgatggatct tttccattac ttttcccgat acaacgactg ctgtcgctac
    10561 actggcagcg gctgctacgc ttaatccgat tgttactttt gttgatgttt tcataagtat
    10621 cagctccttt ttatctatct ccatcatagc atagacaaaa aaataaacga actaatccac
    10681 tcagtgaaat ctcaatcctt tagggaaaaa gttctttact gttcctttta cttgtttccc
    10741 aaatccgaca ggtatcttat ctaatactag caaaacccat ccttgattcc catcacgttg
    10801 aaatgtttct cctgctgtgt aagattgcca ttccttttga gtgatcggca agcaaggtat
    10861 attctctatc ttttttgtag caagcgctaa tgcatagcta ggttcaaatc gattcttttt
    10921 gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag aaggggctaa
    10981 ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt cttccagtag cttcataatg
    11041 aaaatcattt gaaaactccg tccagagttt ttcctgctct tttgtcattt gcactttact
    11101 tttttttcgt gtcttctttt ctttatgcat ctgattttgt ccatgaaaag tcagctttgc
    11161 aacaaaatgt ccttcaccct gatctttatg aggccatata cgtattgttt tttccaaacc
    11221 agctacgctt ccccattcag aacgtcctga actaacggat tgagtcaaag gaatctcctc
    11281 aatcgttact ggataatttt ctacaagcca gctaatgatc tcttcatttt cttctggtgc
    11341 aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt agcattttta ttgctgaaga
    11401 caaaatctct tgctgacgtt tttgacagta tagcggagat tcttctgtcc actcttttat
    11461 tgcatttgga tcttttcgga acattccttc tcctgaacaa ggagcatcaa ccacgatccg
    11521 atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt gcatgatttg tcactattgc
    11581 gttcgaaact ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt tcggaaagat
    11641 ctcattcgtc actaataacc ctttcccctt catttgagca gctaattggg ttgatttacc
    11701 accgggagcc gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag cagctgttcc
    11761 aacgatcatt gcacttggtt cttggctata ttcataccca gcttgatgga ggaaagactt
    11821 tccattgaca gtgccaagaa atccctcatt gctgtaggga gcgggttgta attcttcgct
    11881 atggtaagtt tggaccatgt ccaatcctgc cggttttagt ggattccacc gaaatccttt
    11941 tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc ctaaaagaag
    12001 acgatatttt ttgataaatt gctgtggtaa cgttgtcgct tcctccttca ttccaatgtt
    12061 tcgttagaca ttttacaatc ttttttacga aaaataaagg tggctgatct actttttcat
    12121 gttgctgcct aaggtgactg ttcctttaag atattttcca agtattcatc cgtcacctcg
    12181 tttttatccc aaacagcgtt catcatttca agaaaatctt gagaagaatc atctgagcgc
    12241 aaacttccca atgcattaag ccataccaat tcaagcgact tttcttcttt gatcttcgtg
    12301 tgacctttga catatgagaa aaatggccgc ttaaatagcg aatccatgtc taaatcagat
    12361 tttgcaatga tttcacgaat actttcatgg atagaaaaaa aatctttcaa taagtagaca
    12421 atgcttaaaa catcatcgga actgcattca gctaagtagt tttcaaaata aacagtgatt
    12481 tcttctaata tcttctcttt taacccgctg attgatttga aatgtgtata aatcgggtat
    12541 gtagaacaac cgcagaactc tgctaactct cttgcagtaa ttgacttcat tccatattct
    12601 tccacgtata ccatactttt gaacagcaaa tattctttac tataatttgt tactctcccc
    12661 atcattctta cctccgtctc ttttcatcta cccataaata aataacataa ataattctaa
    12721 aaatattatt gataattatc aaaacatatt tagttatatc cattaaaaat caatttttcc
    12781 gccttaaaaa caagaatatc ttgacaaaat accctaggaa ataaacaata gaataaacaa
    12841 ggtaacaaaa aaattaaata acgttatata aaaaagaagg cgacactctg tgagaataac
    12901 acagactgaa atgactttcc actcacagtt tttgtctaac tatatacaaa aacagaataa
    12961 aaaggaggta tttatgctaa ttgaagcaag aaaaaaagag ttacttgttc tacaaatggt
    13021 cattcaaaaa aataattgtt ctttacaaga attatccaat gacttacata ttccaaaacg
    13081 gacaatcaaa gaactgatcc gaaaaataaa tgtatcactc aagcaatttt tattgattga
    13141 tgaatttatt tattctaatc ataaaggaga gatccaaacc aaagaaaccg atcaaactaa
    13201 aaagttagct gttttttcta agttaaaatt atgctaccta aaagaatcca atcgttttaa
    13261 ctatttgatg ttactaatca attatccaca aactgctgta ccaaaaaaat atttacttga
    13321 acaactttat atatcgccct cctatctcga aaaactgaca aaacagctca atcattcctt
    13381 aaaaaaattc catttacgga tcctatctgt aaaaaattgc tgtactcttg aaggaaatga
    13441 gttatctatc cgtctctatc tttacttttt tctttcagat acatttggag gtaacgattg
    13501 gccattttcc tcacttgaat taaatgcact aaaaatgaac agtacagatg agcaaataga
    13561 aaaaatttca aaacaagaaa aaaatatcta cttgctgtta actttattca tattacgaaa
    13621 caaacaacag gcatattttc caaaaacttc ttctgaaatc actgaattga tggacttatt
    13681 gcagcataca catgattatt cagaatactt tacttctttt tcagtgagtc gcccttgtga
    13741 agagacagaa acagagagag cctactttaa ttattttttc tattgttttt ttccaaatca
    13801 aatctctttt cgacaaaaac aggagattgg ttttttattt tccacaagca aaaaccttat
    13861 ttgtcgacaa gtcggtcaat tgatgactta cttaactcaa cagtttcctc tcaaccattt
    13921 agaaaataaa ataccaattt atttttattt tcttgtgttg gcagttgttt ttttcgaact
    13981 gttgggtgaa tccagcagta gtttttctac actttgcttt ccagaagcca cgtccaatca
    14041 ttctagttct tttatacagg aaaagaaatt aagagaaaaa atcgatacaa ttttttcaaa
    14101 aaatggggaa agaaaccaaa tcagtcaata ccttagtcaa ttgatccaac acttactaca
    14161 aacagaaacg aaagagcagt tactcatcca tttgcagata accaaacagt ttacaggaaa
    14221 ttatcatctt caaagccatt tgcagcagtt atttcgcaaa gatgctgtta aaattaccgc
    14281 atattcagaa catgctgatt tgatcattac agatcagcat gagaaaattt cagatgaaac
    14341 ggagactttc tatctggata caacgcacaa tacagtgtta tttggcaagt taataaaagc
    14401 ggttcatgaa ctgtatatga aaaaaggtta tgagaacaaa acaaagagga tgtgacaaaa
    14461 ttcttgtcac atcctct
//
LOCUS       DACECR010000073        14269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.073, whole genome shotgun sequence.
ACCESSION   DACECR010000073 DACECR010000000
VERSION     DACECR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.073"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(299..1756)
                     /gene="ascB"
                     /locus_tag="IWO17_002022"
     CDS             complement(299..1756)
                     /gene="ascB"
                     /locus_tag="IWO17_002022"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0290885.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(1753..3648)
                     /locus_tag="IWO17_002023"
     CDS             complement(1753..3648)
                     /locus_tag="IWO17_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0290886.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(3706..5481)
                     /locus_tag="IWO17_002024"
     CDS             complement(3706..5481)
                     /locus_tag="IWO17_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0290887.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(5531..7027)
                     /locus_tag="IWO17_002025"
     CDS             complement(5531..7027)
                     /locus_tag="IWO17_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0290888.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(7221..8843)
                     /locus_tag="IWO17_002026"
     CDS             complement(7221..8843)
                     /locus_tag="IWO17_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0290889.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(9171..10484)
                     /locus_tag="IWO17_002027"
     CDS             complement(9171..10484)
                     /locus_tag="IWO17_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0290890.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(10729..10953)
                     /locus_tag="IWO17_002028"
     CDS             complement(10729..10953)
                     /locus_tag="IWO17_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290891.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(10973..11341)
                     /locus_tag="IWO17_002029"
     CDS             complement(10973..11341)
                     /locus_tag="IWO17_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0290892.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(11537..12247)
                     /locus_tag="IWO17_002030"
     CDS             complement(11537..12247)
                     /locus_tag="IWO17_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290893.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(12265..12762)
                     /locus_tag="IWO17_002031"
     CDS             complement(12265..12762)
                     /locus_tag="IWO17_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0290894.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(12799..13251)
                     /locus_tag="IWO17_002032"
     CDS             complement(12799..13251)
                     /locus_tag="IWO17_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0290895.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(13386..13991)
                     /locus_tag="IWO17_002033"
     CDS             complement(13386..13991)
                     /locus_tag="IWO17_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0290896.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
BASE COUNT         4197 a         2978 c         2306 g         4788 t
ORIGIN      
        1 atcagaggtt gtgaaaaagc cgtcctgcat caagtaataa gaacagccaa acaaaaatag
       61 ttcctcatat tttgtcgttt tttgagctaa tgccgatggt cagctgattg tacaccgttt
      121 attgagaggt cgtgagcctg ccgttgaact ccgagtcaca aggagcaatt tcaaaaaaca
      181 gcttctcatg ttttatgact atggtgactt gtgatcgagg agttggcagg agaatactgt
      241 ttattcgtaa agaaggggct gtgacaagac ttttgtcaca gtcccttatt tccttttatt
      301 ataggatttc gccatttgat tggatgactt gtctgtacca atcaaatgat tttttcttta
      361 accgtttctt tgtgccattc ccttcgtcat cttgatcaac atagatatat ccataacgtt
      421 ttgacatttg tgaagtagaa gcgctgacga tatcaatgca gccccacgac gtataaccaa
      481 ataaatctac tccatctttg atagcttcct tcatctgctc aatatgctgc ttcaaatagt
      541 caatgcggta atcgtcttga acggttccat cttcttctac tttgtctata gctccgagtc
      601 cgttttcagc tatgaacagt ggtttttgat acctatcata taattgattc aaagcaattc
      661 tcaaaccctt aggatcaatc tgccagcccc attcacttga tggaagatac ggatttttaa
      721 ttcctccagt tattaagttt ccttggaccc cttcccaatt ttctggatga gcactgactg
      781 cactagacat gtagtagcta aaagaaacaa aatcaacagg gtaggctgct aagatttctt
      841 catcacctac ttctattttt aagttgacac ctttttcttc aaacattcgt tgcgtatacg
      901 ctggataata tcctcttgct tgaacatcac taaagaacaa agaagttctt tgctgctcaa
      961 ttgttttttg aatatcatcc ggatcacaag aatacggaac aagaagctga tagctgatca
     1021 tacaaccgac ttgtgaacct ttgataattt catgacaagc ttttgtagct aaagcacttg
     1081 ctataaactg atggtgtgca ccttggtatt tcgcttgttc caaatgggga tggttttctt
     1141 caattactcc tacgctaaca aaagggtgat gttttacaca gttgatctca ttgaaagtca
     1201 tccaatattt tactttattt ttatagcgtt tgaatactgt ttcagcgaaa cgaacgtaaa
     1261 actgaataag ctttcgatta ggccatccac catattctgt aaccaaatat aacggcattt
     1321 cataatggga aagagaaacg actggctcaa tcccatattt tagacattcg tcaaacactt
     1381 gatcataaaa aaataatccc gcttcattgg gtgtttcttc atcaccatga ggaaaaattc
     1441 ttgtccatgc gatagacatc cgatagcatt taaaacccat ttcagcaaat aaacggatgt
     1501 cttctttaaa gtgatgatag aaatcatttc cgtgtctttt aggataataa tacttgctgt
     1561 tgggatcctg cgccgctttt acttgctgat gcgtcatcat atgccattga tctgtccatt
     1621 tttctttagg taaatcgctg tcgaatgtat acacatcaga cacagataat cctttttcat
     1681 ctacatccca tgcaccttca cattgattgg ccgctgtcgc tccgccccat aaaaatcctt
     1741 ctggaaattt tttcatatcg atccccctaa acttgctagt tttcttgttt gattattttt
     1801 cttatgacta gtaatgatat ttttgaatct actactattt gtaacaataa ccattgtggt
     1861 cggatcatat ccctctttga ttaattcttg caaatctact tccatcaata aatcgccttt
     1921 acgtactttt tttccatcag atactgcact ttgaaagtat ttgccattta aattcactgt
     1981 atcaataccg atatggatca gtatctctgc tccttcttca ctgactaccc caattgcatg
     2041 tttcgttgga aaaactgttg aaacttcccc gtttacagaa ctgtagattt ttccgtccgt
     2101 tggaatgatc gcaaaccctt ctcctaaaga tccactagca aatgttggat cgtttacttc
     2161 agataaatca attatttcac ctgctgcagg agtacttaac actatatcct ttgaggattc
     2221 ttcttcgatt tcatccttgt agccgaaaag aaaagttagc atacaggaac tgatcatcgc
     2281 taagaaataa ctagcaacta gccaaacaaa tccttcacca atataaatcg ggaaagacaa
     2341 aatacttctt gtagcaactg ccaaagcacc ggctcctgcg gcaccagcta ctccaccaaa
     2401 tatagctcca catatacaag caatgtaaaa tggtcgttta tatttaagag tcacaccata
     2461 aacagcaggt tcagtgattc caaataatgc tgtgataaat gcagataaag ataaactttt
     2521 tactttctta tttttcgttt tcaaaaatac cccaagtact gctcctgctt gcgcagctac
     2581 ggcagtcata catgctggac ctagcgtatc acgaccgtag aaagaaagat tgttggtcat
     2641 tactggtaca agtccccaat gtaacccaaa aatcactaac acttgccaaa atccgccgat
     2701 aacagcacca gataaaacag ggctaaagtt ataaacagct aaatatccag ctgcaatcag
     2761 ctgacttgca taatctgcaa ttggacctac aactaataaa ataattggga ccatcacgat
     2821 gatacaaatg aatggtgtca ataaatttcg aatactttcg tgcaagcttt tgcgaatctt
     2881 aggctcaata taagaaagaa cccatacagc taaaattgct ggaataaccg tacttgtata
     2941 ccttgctaga atcacgggca tccctagaaa tcgcaaggtt atgctatcac tatatgcact
     3001 gacaatcgta ggataaacga gagctgctgc aataaccata gcgatgaaac gatctgtatt
     3061 gaactttttt gcagcagtat atgccaaaaa tataggcaag aaatagaaaa atgcatcagc
     3121 tgctgcattc aaaatgatat aagttcctga ttcagtagtt aataaattca aactcgtaca
     3181 taaggcgagt actcctttga tcaaaccact tcctgccaag acgcctaaag tcggggcaaa
     3241 aacagcggcg atcatgtcta taaaagagtt taaaatgcct tttttctcag ttcctctctt
     3301 tccactagat gcatcatctt ctagtttcat ttgtttggat aatacgtcat acacttcatt
     3361 tactgcattt cctactacga cttgaaacat tcctccagct tcgactacag tgatcacacc
     3421 ttctagttgc tctaccttct ccttatctgc cttcttatta tcttttaggg taaagcgtaa
     3481 ccttgttgca caatggtaaa cattgctgac attttcttcg cctccgacaa atcggacaat
     3541 atctttcgct aattgtgaat agtccatttt tacttccccc ttgttaatta atgccgtagc
     3601 aatccttctt acaagataag tatagttctt agaaaacggt ttctccattc aattagtacc
     3661 ccttctaata gcgaacgaaa ctgtacaata gtgtgtatgg tttacttagc cgagcaaatc
     3721 actcactgct tgaaagaaaa caagtatatc gtttctctta tagtcaaagg agacaaaaaa
     3781 aacacgcttg atcttccttt gctgatgtat aagtggtatc tcaagctgat actctgtgat
     3841 ttttgtttct acttggcttt tgataagaca gcacaacaaa gtctcggcat ttaacggaag
     3901 cataactagg tcagaactag gagagattag ttgctgaatg gaatctatct ttttatggat
     3961 ttttatttga ttttcttctt tgtagtaatg atcaagcagt tttgttttct ttttttcttc
     4021 aactatggtc agcttttcac gcaaaatctg ataaatctcc caagattcaa actcatctag
     4081 aacactaggt agataaatca aatcatccca ctcaaaaata agctcttgct cagtagtcaa
     4141 aattgcatca tattccgtta ttttgtttga ttgatttttg aaaagataca ctggttctat
     4201 ccggcattct ttgatccaca tgttcatact ttgttgaatc tgttttttta gtgtattcaa
     4261 aatactaatt ggctggttac taacaatcag tatctcccct tgttttcgaa aaggattcaa
     4321 cgcttcggca agatacaaaa caaaataagc gctttcagct aaattgggac gtaattgaaa
     4381 atgtattggg aagaaacgcc gaattaaata aatttctaaa ggataacggg agatgatctt
     4441 ttgtacaaaa tgattcatgg tattatgtcc ggcatccatc ctcgttttca tttgtttgat
     4501 atgggttagc aaaaattctg tttcatggaa caataacgaa acaggaagat ctaaaaaacg
     4561 aataatcgtt tgctcaaatg ctcttagatt cttctctatt tctttgtttt tttcatcatt
     4621 aatcatcaaa taatttaact ctaatagtcg attttcaatt gcttgttttt cttgattgga
     4681 aaaagaatag ggtagttctg atgataactt ttttgtgagg gtctctacca tcggaataaa
     4741 taatgtatac ctgtttgttt tttctaaaag ttcttctctt cgaagaatgg tcaaaacgag
     4801 ataccgagaa aatcgtatga agtcctctcc tgaaacaata taattatatg atataagaac
     4861 ctcttttaat gtcttttgga cagtcggcat gatattggaa aaaattgtac tgatttgttg
     4921 aggaaacctt gcgtgttgga tcaccgtttc tgttccaaca agagataaga aaattctttt
     4981 tgaattttct tcagcaatga tttttagtcc ttttgtcgac gaaacttcca gttctaattt
     5041 aggattgcgc tcgatccaac gcaaaatcgt ttttatttgt aatgatacgg ctgtttttga
     5101 aagaaagaaa atgtctgcta tcttttgcat actgacatac cctttttgaa acaataagta
     5161 cgtcacaata gttgaagctc ggaaatccat gctggaatag attttatgtt tcgtcacctc
     5221 ttgtatgatt tgctgtttag tttcagattc aattgattct aaaaaataac ctgaacgttt
     5281 atttgactgg ataatgattt tcttctcgta gatagcaagc atatttttga tttcagtttg
     5341 gatcgttttc ttgttggtac ctaaagcttc agctaattct tcgcttgtta tccaatgatt
     5401 cttatttctt aatactacaa gaatttggag ttgtcgttta tttaagtcca tttgcttaca
     5461 cctcctcttt gttataaaca tacaggaaaa acctctcaaa tgacaatagt cacttgcaga
     5521 ggtttaaaaa tcatttgaag aaagatctat attcaaggtt tttaccatta gactggataa
     5581 tttgtttata atattcaaaa ctatcttttt tcaatctcct aaagcttccg ctcccatcat
     5641 tttgtctatc cacataaata aagccatatc gcttcctcat ttcccctgtt cctgccgata
     5701 cgatatcgat tggtccccac cataagtaac caatgatttg gcaaccatct tttattgcct
     5761 cagcaatttc ctgaagatgt atttttagat actcacaacg ttttggatcg tggattcgat
     5821 tcaattcatc tggtgcttca tctagtccaa tgccattttc aactatgaat aatggtaatt
     5881 cataacggtc tgtcaattca ttcatgacat aacgaaaccc tagaggatca attggccaac
     5941 tccaaggttc gggagatttt tccttcaaaa agggattatc gaaaccattt gctccgccag
     6001 tatctacgcg catttctgct tcttttttat agacagcgct acgataataa ctaaatgcaa
     6061 tataatctgc cgtgttagct tgcaagcact gttcgtctcc atctaacatt actggtgcac
     6121 agtcttcttc cttccatgtc ctgtaaatat aagctggata atgccctcgg cacatgacat
     6181 cggagaaaaa ccatgaattg cgtttaaatt gcagtgaacc aaatacattc tctggatcac
     6241 agtcatatgc ataagtcgct aatgcagaaa agctacacat aatacccatc ttagcatttg
     6301 gatcgatttt ctttaataat tgagtagcac gggcgttcgc tagaaacata tgatgacagg
     6361 cttgataaat cacttttttt gttagtgtag caatgggttc aagtttatct ctagcaggga
     6421 atgtaaccaa tccagcagct gcataaggca tacgaaaagc attattgatc tcattaaagg
     6481 tcatccaata tttcactttc ccttggtagc gttcaaagac ggtctgcgca taaaattcaa
     6541 aaaagtcgat taattttcga ttttgccacc ccccatactc tcctaccaat gctaaggggg
     6601 tttcataatg actaagagta attactggct cgatatcata tcgatttaat gtattgatta
     6661 gtctgtcata aaatacgaga ccagcctcat ttggcttctg ttcgtctcct cttggaaata
     6721 ttcgtgccca tgagatgctt gttcgaaaag cttttaaacc taactccttc agtaaacgaa
     6781 tatcctcttc aaatcgatga taaaaatcaa tcgcttcatg agaaagataa tggagtgatt
     6841 cgtctaaagc aatctcccaa atcttttttt cttcattcca ctgaatacta ggatgctggt
     6901 tcatgatccc gataagcgta tctgtcacat taggtaattt gccgtcttcc aaccatgcac
     6961 cttcacattg attagcagct actgctccgc cccataaaaa attttctgga aatatttcct
     7021 gatccataac tatctctcct cgctatcaac aatttcttac ttgaagtata gctcgattca
     7081 tcaaaacaag atattctaac tatttcggca gaagagaaga aaaaaattga atgctactct
     7141 tcatttttaa tatttatata taacaaatac ataagacgat ccccgaaaga aaacactcgt
     7201 taaccatcta agcaaacgat ttattttact tccaacacaa atgcttcata aggtcgtaaa
     7261 tcaactttgc cgtctggtaa ctgtttcaga ggataatttg acacaataac ccttaaagct
     7321 tcacgtggaa tagtgaacgt ctgcttttca ctggacatat tacaaataac gagccattgc
     7381 tggtcttcat atatgcgaga ataggcaaag acatgctcag gcgtcttttc cactaattca
     7441 tagtctcccc agacgatgat gtcatgtttt ctacgcaaat cgatcaattt cttgtacata
     7501 taaaaaacag aatcttgatc tgctaacgct tcttctgcat tgatttcttt ataatttgga
     7561 ttgactcgaa gccatggaat tccagtagta aacccagcat gttcacgggc gttccattgc
     7621 ataggcgtcc tagcattgtc tcttcctttc gccttgattg attccaagat ttcctctttt
     7681 gtaaatccag aagaaatcct ttcgttatac atattgattg tctcaatatc ttttgcttcc
     7741 gcgatatcat cgattgggca attcgtcatg cctaactctt ctccttgata gatataaggg
     7801 gtgcctttca tcatatggag caagatagca aacatcttgg cactttcttt tcgatacaca
     7861 tcgtcatttc cccaacgaga aacaattcgc ggaagatcgt gattattcca aaataaacta
     7921 ttccatcctt catcacctaa agatgtttgc catttggata atactttttt cagttcagag
     7981 atactcaagg gtttcaaatc ccatttgtct ttgccttctt gctgatctaa tcctatgtgt
     8041 tcgaattgga agaccatgga taattcattt cttttaggat tggaataaag tttagcgatt
     8101 tcggtagttg ctccccatgt ttcgccaaca gtcatcagat ttttgtcacc aaaagttgct
     8161 tgattcattt cctgaagata ctcatgcagt ttaggtccat tacttgtgat ttctttatcc
     8221 ggttctttgc caatcatatc gatcacgtcc atacgaaagc ccccaatacc tttatcgatc
     8281 cagaaattca tcatttgata aatctcttgg cgaacctgtt tatttttcca attcaaatca
     8341 ggttgacgct tgctatacaa gtgcagaaaa tattgccccg ttttttcatc caactgccaa
     8401 gcagaaccac tgaatattga gcgcaatcta ttaggctctt ttccattgac cggatcacgc
     8461 caaatataat agtcccgata aggattttct ttatttttac gggcctcaat gaaccacgga
     8521 tgttcatcag aagtatgatt caccaccaga tccatgatga tacggatatt tcttttcttt
     8581 gcttcttcaa tcagttcttc catttcttcc atcgtaccga attctgccat gattgcttga
     8641 taattactga tatcataccc attgtcatca ttcggagact cgtatactgg agaaagccaa
     8701 attgcatcaa tacctaaata cgccagataa tccaacctgg aaataatccc ccgcaaatct
     8761 ccaataccat cttgatttga atcttgaaaa cttcttggat agatttgata aacaactgaa
     8821 tggtgccacc atttttgttc catgattttc ctcctataaa tggatgtttc tattttttgc
     8881 ttaatcattt attttagtct ctctcactaa aaattgtaac gctttcaaat agctattgca
     8941 ccttgttatc ggtaacagaa taaaacaaaa aaacagctga ggaaattccc tcaactgttt
     9001 tttgaaagga ttgatttatg cagggaagaa tgctgatttc acaacttctt tataaacggt
     9061 gttcactcag ctgtcccttg gtattagctc aaaaaacggc aaaatataag aaactatttt
     9121 ttcgtttttt tctctaatac tcaggccaga acgctgattt cacaacctct ttatttcaca
     9181 ttcaatatcc acatgaatcc cacaaataca aagaacagac catacatgat tggagatact
     9241 tcttttcctc ttttagctgc taacatggta attggataga agataaaacc taatgcaatc
     9301 ccatctgaaa tactataagt caaaggcatg ccgatcaaga tcaagaatga agggatcgca
     9361 atctctaagt ttccccattc gatttgtcgc aaagattgtg ccatcaatac tcctaccata
     9421 atcaatgctg gtgcagttac ttgtgaagta acaactgata gtaatggaga aaagaacaag
     9481 cctaacaaga agaaaatccc tgtagtaata gctgtgatac ctgaacgtcc tccaacggcg
     9541 ataccagctg atgattctac aaatgcacca acaggagaag taccaaataa ggaacccgca
     9601 agcatagctg ttgaatcaga agctaaagct ttccctacac gaggcatctt gttgtctttc
     9661 atgaaacctg cttggtttgc taatccaact aaagttcctg ctgtatcaaa gaaagtcaca
     9721 agcaagaatg tcagcactac gacccacatt tgaagagaat tgatgtcccc tacatgtttt
     9781 aatgcaacta aaaatgttgg tttcaaactt ggtgctgcgg caacaatttc agaaggcatt
     9841 ggaatcaaac ctgtgaccag acctaaaatc gttgtagctg ccatcccgat gaagatgcca
     9901 cctggtacac gccgaacaag caagatagca gttacgacta atccaaaaat cgtcaaccaa
     9961 gtagaaccaa cgttgagtga tcctaatcct actaatgtag attcattcga aacgatgatc
    10021 ccaccttcgc tcagaccaag gaaagcgata aatagaccga taccgccaga aatcgcatat
    10081 ttcaaatctg ctgggatcgc atcaatgatc atttcccgca gcttgaagat tgtgatcaaa
    10141 ataaaaatca aagaagcaac gaatacgccg gctaatgctg tttcccatgg aatccccatt
    10201 cctaaacaaa cagaataagc aaaaaaagca ttgatcccaa gagcaggtgc cgttccaatt
    10261 ggataacggg ctaatacccc cattaaaaca cagccgatcg cactggctaa tgcagtcgca
    10321 gtaaacactg ctccttcatc cattccagaa gcacctaata cagttggatt tacaaataag
    10381 atataagcca ttgagataaa tgttgtaaat cctgccagta cctcacgttt taccgtggtg
    10441 ttcaattttt ctatttcgaa gtaggaaagt attttttcct tcatcttcca tccccctaaa
    10501 tccgaacatt ataaataaag tccgttaaaa tttcgattta ttaataaaac aacgtgtttt
    10561 atcatatact aaaacaaaac ataagtaaac aaaaaattcg gattttaagc aaaatccgaa
    10621 cgttccatct gattaaatcc ctttatctca aagttcatga acattcactt tggattacaa
    10681 gaatttcttt attgtgttaa taaagtttct tcattctagt ctcaaaaatc aatagtgtga
    10741 tttgatttta ccatttcgtt tctgttcttc tttgatgaat tcttttgtat cgacaggatt
    10801 cagattattt agtccgccaa aacctgtatc gaccattttt ccccagcctt ttgtcttctt
    10861 ttttcctgat ttgagattct ttgctcgaag cttctcacta tgttttgctc gccgatactt
    10921 caaataacct tctaatcttc ccattagatc cactgttttt cctccttttt attcaatcag
    10981 acgactcagc ttttcctata acaaaataat tgccttcagg gtcagaaaaa ttgaaagtca
    11041 agatgccatt gttatctgtg atttctcctg gttcttccat gttctcgtag atagtttcaa
    11101 attcatccgt aaaaaatact aaagaaggcg gtggtcctaa tacctctggt gaaaacttct
    11161 gcacaaatac ttttgggaat acgcctaatt ctaattcttt tgagatgctg agtacaatac
    11221 tcttaaattc ttctggcaat tcgattgttt cagaaatttc tgcaccaatt ttttctacaa
    11281 agaatcggca aatcatctct acgtcattga cataaagcat cacacgtgtt ttttgtatca
    11341 tcatttgacc cttctttcta tttgattttc tttcctgtct ataaactgat tccattatat
    11401 cacgaaagcg caaacacctt gtgtttgata ctattcaaga aaaataaagc aatagtttct
    11461 aggattgctt tcctccacaa acttttttta caaaaaaaaa acggcaatga atgccgctct
    11521 tcttatttcg attcttttat aattctgctt ctgattcatc tttggttagt gtgtgtctaa
    11581 cactagttac gccaaatgtt tgatcgattt tttccttcaa cgcttcgatt gcctgtgcgc
    11641 cacgtgccgc ttgttcagca ttgatggatt caatgaccag caccgctgct tgtgtttctt
    11701 ccgtcagcat cgttctttgg gcttcacgcc agttgagaca gcgacagatc gctccttgtt
    11761 catcatagta gatgatttct tccggaagtg ccggagcatc ggaatctgca cctaaaggca
    11821 ggaatgattc gccaccttgg gcttttccta aatgcagatt tcctgcgatc ttttcgatat
    11881 cttctccgcc gcaaggtacg ccaaaagaca aggaaatact attatagata tctacaagcg
    11941 gattgattgg gaaaaattcc cgttcttgat ttgcacgttt cagtaaagct tcaatagaag
    12001 aacgtgcacc ttttttagtc ttgaatgtgg taaatgcctg acgccactga gcaatgactt
    12061 cgttttggct gaatgtttct tctgtaaggt aatttcttgc ttcttttgct gcctcattca
    12121 ataattgatg atacgtttct tcactttctt ttgttacgtg gttatcgata ccatttacgg
    12181 ataagatttc gatggttgct tcaggaaata cttcccagaa attggtatcc actattactt
    12241 ttttcatctg ttcacacctc ttgattagaa taattgcttg atgaatagat agtctgtttg
    12301 gggatcatct cccatataaa aaacatgttc gctgtatttg ataaatcctt ttttacgata
    12361 aaaagcgagc gcaacatcgt tgtgttccca aacaccgagc caaatggctg ttttttgctt
    12421 ttcttttgct ttttcaatag ctgtatctag taaaaatgta ccgatacctt gatgtttaaa
    12481 tttttttcgt acatagattc gctcgatttc taaaaaatga tcagccattt cctcagattg
    12541 actcgttcct gtatttagtt tcaggtaacc cgcaggctct tgatcgatac tcactaggaa
    12601 aaaatcggtt tctttatctt ttagctcttc tgtaagtttt tctagtgcat aagcttgtgt
    12661 caagtaatca gctaaatgct ccggtgaatt atcttttcca aatgtatctg taaatgtttc
    12721 gatactaatt tgttgtagta agtctacatc tgaaggcgtc atttttcgaa tttcgtattt
    12781 attcacggct tcttcctttc aatagatcct tttattgcct tttttgacaa aattccaatc
    12841 ttcgctaaca ttcttgcata ctttatttaa aagatattcc aattgttttg cttcttcgtc
    12901 agaaagacca tttaatgcga ctgcattgga gtagttgttt tctcgaatga tgaaaggata
    12961 gatttctgct ccttttttag tcggatagag tttcttgatt tttttatttt ctttatcttc
    13021 caaacgttcg atcatcccat tggattctaa tttcttgatt gctcgggcag tcgttgtacg
    13081 atctactttg atcatttctg ctagtttttc ttggatgatt ccaggatttt cgcaaatacg
    13141 tactaaataa agatactgtc ctcttgttaa atcgacttct ttgaattcga tattgctaat
    13201 agaatctagc gctcgggcaa tcattccgat gtctcgaagg atttcagcca tttcatccca
    13261 cctttatttt gtttgtaaga ctagttcacc tctttttgtt gcaaatgcaa ctattttcaa
    13321 taaaaaaatc aagttctttt ttaataacta gtaaagtata ttcttttctt cttttacgaa
    13381 aaagctcaat cacaatagat ctggtttttg ttttctaaaa taagtaactg tttcttcgtt
    13441 gttcgctttt ctgatagctt tggcaactct tctatactaa aaaatcccat gttggaagtt
    13501 tcattattct caataaactt ggcctcttca ccgtggatcg cacaagcaaa aacaagttta
    13561 taatactgaa aaagctgtgg aatatctttt tgtttattcg tatcaaaaac tgctcgcaac
    13621 tgagtagttt ctactgttaa tcctgtttct tcatagactt ctttacgcac attttcttca
    13681 ggagacaatc ctatttcagc aaatccacca ggaagtgacc attctttcgt ccgtttatct
    13741 tcgattagta ggaccttgcc atcttttttg atataggcac gaacatctac ttttggtgtt
    13801 ggatgtcctt ctccaatcgt tgtgagcgct tttatttctt cgaaagtttc ttttcctatt
    13861 tcactgatca gttccaatga aatcgtccgg atctcctcaa aacgctcttt atcaaataca
    13921 tcttttccat aatatagacc agcttgagaa agtgcgagta gtcttttgta ttgagccaac
    13981 ttcttgttca ttctcttccc ttctttttta gcaaatgtat gactatgtaa tcaagtatag
    14041 aagactggtt taattacggc aaataaatat aatgaaatct gcaataaata aaagaatgtt
    14101 aaaaaactcg agaacaaaag acattcgact tctctggggc tgtgaataaa tggcgttcaa
    14161 aagcttgcat ctgcggtaat aagccccacc aaacgaaaaa tatgacgagc tattcttgtc
    14221 tggctgttct tattaattga ggcaggacag ctttttcaca acctcccca
//
LOCUS       DACECR010000074        13743 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.074, whole genome shotgun sequence.
ACCESSION   DACECR010000074 DACECR010000000
VERSION     DACECR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.074"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(27..683)
                     /locus_tag="IWO17_002034"
     CDS             complement(27..683)
                     /locus_tag="IWO17_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0290897.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            876..1154
                     /locus_tag="IWO17_002035"
     CDS             876..1154
                     /locus_tag="IWO17_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290898.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1173..2381
                     /locus_tag="IWO17_002036"
     CDS             1173..2381
                     /locus_tag="IWO17_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0290899.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2529..2894)
                     /locus_tag="IWO17_002037"
     CDS             complement(2529..2894)
                     /locus_tag="IWO17_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ0290900.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3041..3814)
                     /locus_tag="IWO17_002038"
     CDS             complement(3041..3814)
                     /locus_tag="IWO17_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0290901.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3988..4848)
                     /locus_tag="IWO17_002039"
     CDS             complement(3988..4848)
                     /locus_tag="IWO17_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ0290902.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4848..6266)
                     /locus_tag="IWO17_002040"
     CDS             complement(4848..6266)
                     /locus_tag="IWO17_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0290903.1"
                     /translation="MKIVGVTACPTGIAHTYMSVEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6323..6772)
                     /locus_tag="IWO17_002041"
     CDS             complement(6323..6772)
                     /locus_tag="IWO17_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0290904.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7217..7480)
                     /locus_tag="IWO17_002042"
     CDS             complement(7217..7480)
                     /locus_tag="IWO17_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ0290905.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7579..8874)
                     /locus_tag="IWO17_002043"
     CDS             complement(7579..8874)
                     /locus_tag="IWO17_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ0290906.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8871..10142)
                     /locus_tag="IWO17_002044"
     CDS             complement(8871..10142)
                     /locus_tag="IWO17_002044"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0290907.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10307..11176)
                     /locus_tag="IWO17_002045"
     CDS             complement(10307..11176)
                     /locus_tag="IWO17_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ0290908.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11468..13078)
                     /locus_tag="IWO17_002046"
     CDS             complement(11468..13078)
                     /locus_tag="IWO17_002046"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ0290909.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3912 a         2894 c         2342 g         4595 t
ORIGIN      
        1 cgctgatttc acaacctctt tgttcatcaa aagataatat tgtcttctaa caagtccata
       61 ttcaaaataa cgatatgatg gtctttaacg gtgataatac catctttttt cagttgtttt
      121 aaaattctgt ttacactgct agcagttgag atgccacaaa atttggcgat ttcttcattc
      181 gtgatcgaaa aatcgatcag catcccttct tctgtccgca caccgaataa atcatataat
      241 tcgtaaagtt gcgtacaaac agctcccagc tttccgttta atagcatttg ctgcattttt
      301 ttcatcgaat aaagcagacg atagcggtaa tactctttta catagtgttg tagctttggg
      361 tcttggttga tattttgcca aaagcgcaca cgattgattc gataaaactc tgcatattct
      421 gattcaactc gaatattata cggcgaatcg atgaattgtg aatattcgtc ttttaaaaga
      481 gagacgattt caagatcttt gatatagcga aggttaaatt ctctcccatc atttgaaata
      541 acacttgctt ttaccgttcc ctctttcaag atatacgtat gggaatcttc catcccttcg
      601 aatgtcaaat atttatgata tcctttgcgt ataatcggaa aatcattttt atccaaatat
      661 tcctttaaga ctctactatc caatgatgct cccccatcca aactttcatc taaaaagatc
      721 atacagtact ttaaaacttt ttaaagtcca aaggtatttt actaatgata ttaacatttg
      781 ttaagtacct ttttccatca cttatgcgat ttttttcctt ttttttattc gttatactgc
      841 agaagtaaaa ttttttaaga atagagggaa aaaacatgaa ttactggatg aacacaatca
      901 ttaatcgatt agaaacagca tatcaaacaa gatttgatat gaaagcttct ttagtctttt
      961 taaatgatgc gtatcaaaac tcgatcgaat tgatcaaagc agtggatgag aatccaacaa
     1021 acgaatgcga agaattttta aatctgttta tgtctacaag agatttattt attcgccaac
     1081 ttgttgatcg ctatccatcc aattatcacg atgtagaagt tcaaatacaa aaattaaaag
     1141 catattcagc ttaaggggaa tgtataacat acatggaaaa gaaattattt aataaatatt
     1201 ttattggaat cacattgatc aattttattg tctacctgat ttattatctg ttgatggtca
     1261 tcatggcagt cattgcgcaa gacaccttac acgccagctt gagccaagca ggttttgctt
     1321 ctgggatcta tatcatcgga acattgctgg cacgcttatt catggggaaa cagttagaac
     1381 tatttggtcg gaagtttacc ttaagaggcg gcgcgatctt ctatctcctt tcaacattag
     1441 catatcttta tacgccaact atcggggcat tgtatttagt tcgattcttg aatggattcg
     1501 gctacggtac agtttctaca gctacaaatg ctatcgttac tgcatatatc cctgcatcta
     1561 aaaaagggga aggcatcaat tattatggat taagtactag tttagctgcc gctgttgggc
     1621 catttttagg aatgattttg ctgaatatga ctgattttcg tttcattatc tggttttcaa
     1681 ttgtactggt cttctttgtt acgattgcct gccttgtctt tcctgtaaaa aatattccat
     1741 tatctgaaga acaaaaaata tctcttcgct catggaatat caatagtttt attgaaaaga
     1801 aagcactttt cattacagga attgcctttt tgatgggtct ttcttattca agtgttttat
     1861 catttttatc ttcttataca aaagtaatcc acttagttgc tgctgggtca tttttcttcg
     1921 tcgtatatgc tttggtcatc acatttacac gtccattgac tggaaggatt ttcgatgtca
     1981 aaggagaaca atacgtgatg tatcctagct atttattctt aacagcagga ttatttttac
     2041 tgagtgtaac gacgaatagt gtgacattgc ttatctctgg agcattagtc ggcctaggtt
     2101 atggtacgtt tatgtcaaat ggacaggctg tttgtttaaa aatcgtccgc gaacatcgaa
     2161 tcagtattgc tttatctacc tactttattg gtttagacct aggcttaggc gttggtcctt
     2221 atttgatggg gatgctaaaa tctcttactt catttagagg attgtatgtg attgccggag
     2281 tcattccatt gatatgtaca gcactctatc ttgttaatgg ccgaaaacat agtcatgaag
     2341 cagagggcga attgattgaa tcattgaaaa atgaaatttg atcttgatga atagataaaa
     2401 aaaaccgttc agatctgaaa ataatctgag cggttttttg agtaaaaata gtttatgatt
     2461 attcttaatt aaacatctat ttatttttaa agctttcatt tatcgataag aatagtttaa
     2521 ctgttgattt attccatttt ccgttcatct ggtacgatgc cttgacttag caaccacttg
     2581 tggaagaacc attctgccac accgatcaat agagcaggaa tcaatacttt catccaaata
     2641 tcgactgtat cattcataaa atatagatag agccacaaga taactgcagc tgaaccagca
     2701 tctgcgatcg tcgctgcgat gttccctact ttacgtaaga tcaacagatc ccctagcaca
     2761 tagatcatga ctacaataat taccccaaca atcgttgaat ccataaagct aacaccgtat
     2821 aaacctgtca gcacgaccca cagaatcgcc cagatcatga ttgccttcac taccaacgcc
     2881 ttcacatgtt tcattttctg cactcctttc tcctggataa aaaagtaaga ttatctaaca
     2941 agataacctt ttcattatta taccatcggc tgagagaagc gagcaaagat aaggtcctat
     3001 ctaaaaatga ggaagccact tagaacattc acgtaataat ttatccgtac agacattcga
     3061 tatggtgttc ctttactttc gcatactgtt cttttgtcag ttctttactt gtcaccagat
     3121 aatcgatttc atgaaaagca atggcttgat gcatgaattt atgtgcaatt ttttgctgtg
     3181 taactaaaac ggcaagccgg tctgattgtt ttctaaaagc ttttttgacc attgcctctc
     3241 cttcatgagt ttctgttact ccttggctga aatctagccc gcgacaactc attatcgcta
     3301 aatccgcata atatgacaat gcatcgttat aggcttttga accgttgatt gttgctcgtt
     3361 tcggaacaat tgcccctccc aatacagata cttttgccgt tgtatattca ctcaacatag
     3421 cagctgtgac gatgccattc gtaatgatct gcaaaccatc aaatcttgct aggtttctga
     3481 ctaagtgtaa agaggttgta ctggaatcca agaataagac catatcatct cgaatcaaag
     3541 aagaagctgt atctgcaatc ttacgttttt ctgtttcttc ttctaatttg gtgatcttca
     3601 tcaggtaagg cacgtcaatt tttcgattgt caataagcat ggcgccaccg cgaactctac
     3661 ggatcaatcc acttttttcc agaaaaacaa ggtcacgttt aatcgtggct tcgctataat
     3721 ttactaactc gatcaaatcg gtgactcttg caaattggtt gttttttaga taattcacaa
     3781 tctcctgctg tctattctcc acttcattca acataaaatc accccattta ctttatatag
     3841 taacaggaaa aaagaggttg tgacagaagt gattggcttc gagaaataag aagaaatttt
     3901 tcgaaaaaac tacttctgaa catcgtgtgt ggttaagagg gtatgacaag actattgtca
     3961 taccctcttg accacacatt tttattttta gtataaacta gccttgccta ctgtgttcaa
     4021 aatttccatt ttatgtttga ccacttcttg taccttttta ttagctgctg gataaattgc
     4081 attcggctcg tacatcgatg gattttcact caatatttct ctcaaagcat tgaaaaagac
     4141 gcttttaaga tctgagctca aattcacttt ggcaactccg tattttactg cttcgctgac
     4201 ttctgcgtcc ggattacctg aaccgccatg taaaacaaat ggaagatcga tttgttggtt
     4261 caattctttc agtaagtcga tatttaattt tggagttctg tttttcggat acaagccatg
     4321 cgctgttcca attgctacag caagtgtatc gattcctgtt tctttcacaa aaatcttcgc
     4381 ttgatcagga tctgtataaa tgattgtttc agcacctcct tcagcagaac cattattccc
     4441 aatcgttcca agctcagcct ccactgagac atttaccgca tgagctaacg caaccacttt
     4501 tttcgtaatc tgtacattgt cgtcgaatga tttcaaagac gcatcgatca tcacggaaga
     4561 atagccgttt cgaatcgccc gtaagacatc ttctatcgta ccgccatgat ccaagtggat
     4621 aacgatcggc agtttacttg tataagcata ggctcttact gccgcaacaa actcatcact
     4681 taaataggcg atttcatcag gatgaatttc taagattact ggtgcttcta atctctctgc
     4741 ttcttcgatg attgctttca tcatatcata gctgcaaata ttgaaggctg gtactgcaaa
     4801 ttgattctct ttagccactt tcaataactc tttcattgta tataacatta cgcttccacc
     4861 tcatttgtta gattttcatt tttcgtccac ttgtcttgtt ttttctcttt cactgttttt
     4921 ttcaatagtc caagcataaa ggctgtagtg actgtacctg atgcaattgc caagacatac
     4981 ataggtaaat tgttcataac atttggaatt agcatcaccc aaatccctcc atgaggtgcc
     5041 attgaagtaa ctcctactgc catggaaatc cctgcagcga ctgctgaacc tgtcatcaaa
     5101 ctaggtatca cgcggatcgg gtcagatacc gcaaacggta tagcaccttc tgtaatatac
     5161 gaagcgccta aaatccagca tgattgacct gaagtccgtt cttctttggt aaatttatgt
     5221 ttgaataata aagtggctaa agcaatacct aggggaggtg tcattcctgc agccatacaa
     5281 gcagcaatcg gtgcatttac tccagctgac atcaaaccga tcgagaaagt cgaagtcgat
     5341 ttattgatcg gcccacccat atcagctgcc atcatgatcc cgataagtaa tccgaacaat
     5401 agcccggatg tatttcccaa gctattcagc caatttgtca aagcatccaa cacccatgtg
     5461 atcggttgtt caatcacaaa taccatcaaa agcccaataa ttaagatcga taaaaagggg
     5521 acgacaatca gctgataaat agcagaaaat tcttttttta cgctaatttt atttgccaga
     5581 aaatcacaga tataccctgc tagcacgcca ccaatcattc cgccaagaaa acctgaacca
     5641 cctgtagagg ccagtaatcc ggcaacaatt cctggggcaa aacctgcttt tccagcaatc
     5701 gatgtagcaa tccccgcgcc taacgcagga accatcatgg caaacgctgt atctcctccg
     5761 atccttgaaa atgcagctgc taacacatta tagtctgctg agttgggatc agccgctgta
     5821 atcccaaagg caaaactcag tgcgatgatg attccgcctg cgatcacaaa tggaagcatg
     5881 taattcacac cattcatgaa atgattatag attgtcggct ttttctcatt tgcagctggt
     5941 tctttttcat tagacacaac gaatacgcca atatctctca atgcttcgtc tattactact
     6001 tgcggttctt tgattgctcg gcttgtagag acacgcttta cttttttccc ggcaaatcgg
     6061 tccaattcga tttctttatc tactgcaaga atgattgcat ctgcctcttt gatttcttgt
     6121 tcagataact gattttctgt ttttacacct tgtgtttcga attttgcttg atggtttaga
     6181 tttcttgctg ttacttctaa tttttctacc gacatataag tatgagcaat tccagtcggg
     6241 caagcagtta caccaacgat tttcatgatt attttcctcc tttttcttca ctatcgatac
     6301 tcggacaaag actctactat tttcattctt gactaatctg tctgatgata tctgctggcg
     6361 tagtggcttc ttttattccc tttctaaaat ctgcattcat caatcgtctg gagagtgtgc
     6421 tgagaatctc tagatgcata tctgctgatt tttcaggaac tacgatggca aaaatatatt
     6481 caattggcat ttcatctatc ccgctatagt ccgttaattt ggctgtatga aaaaaacaca
     6541 gtgctggttt tgtcacatat ttgctttttg catggggaat cccaaatcca tcttctattc
     6601 ctgtggagat ctcctcctct cgtttgaata gtgccttaac gactttttga acatttctta
     6661 ccttatttgt ttttttcaac acatccccta ttcgctcaaa taatgatcct tttgaatcta
     6721 tcgatggatc gaaaacaatc agttcttctg cgatcaattg atcaaacctc ataaaaaacc
     6781 tctcccccac aaaaaatgtt aacgttttct tggaactgag gtcagtatag catcgctagc
     6841 tgtaccgttc aatattatga atgaacagag gaaaaagacc catcatccgc gtgtataagt
     6901 cattaaaatg aacgatctag ctcacaaaca atcgcaagtc tataacaaaa caaaaagcct
     6961 tccttgaaag gaaagcttag cttctaattt taggtaaatg gttttttact tcgaaataac
     7021 ccctcttcat ttcgttaata taaggtgttt ttatcaagtg gataattaaa actatatact
     7081 agtttttcat tgttttgtgc attatgatcc tacgactttt tcagcatcaa acgaagtttt
     7141 aaattggcaa aaaacataaa aaaacagact atccctatca gatagtctgc taaacgttga
     7201 gttatcagcg tttttattat tccgcagcgt ttgcgtcttt gagaccgtat tttttgttga
     7261 aacggtccac acgtccgtct gcttgtgtga atttttgacg tccagtgtag aatggatgtg
     7321 agtcagaagt tacttcgaca cggatgactg ggtatgtatt accgtcttcc cattcaactg
     7381 tttcttgtga agtttttgtt gaacctgaca agaatttgaa accagttgtt gaatccatga
     7441 atactactgg gtggtaatct ggatggatgt tttctttcat gattttttcg ctcctttgcc
     7501 ctgctccatt tgctgaacca gagttgattt gtcgatttac aacattaaca tcttaacatg
     7561 tttcagtcaa ggttgcaatt atcttttcag atttctggtc ggattttttt taccaaagga
     7621 gacatcttga aattctttga aaaattgctg attgtttttt gttttacgca aaaagcggat
     7681 cagttgttcc gtatattcta atgaatctcc agtcatattt tttcgaagtt tccaaatttc
     7741 ttctaattct tcacttggca tcaataattc ttcttttcgg gtacctgatt ttttgatatc
     7801 gatggctggg aagatccgac gttcagataa ttcacgtgaa agttgtaatt ctagatttcc
     7861 agttcctttg aattcttcat agatcacgtc atccatacgg cttcctgtat cgactaaagc
     7921 ggtcgctaaa atcgtcaaac tgccaccttc ttcgatgttt cttgctgccc cgaaaaattt
     7981 cttaggttta tacaatgcgg ctgggtcaat cccgccactc aatgtacgtc cgcttggtgg
     8041 aacgactaag ttataggccc gagcaagtct agtaatacta tccatcaaga taacgacatc
     8101 tcgtttatct tcgactaaac gcattgcgcg ttccaatact aattcagaaa ctcgagtatg
     8161 gttttgcggc tgcagatcaa atgtagaaga taccacgtcg cctttcacgc ttcgttctag
     8221 atccgttact tcttccggtc gttcatcaat cagtagaacg attaattcta cttccggata
     8281 gttttcggtg ataccatttg cgatttcttt taatacagaa gttttccctg cttttggcgg
     8341 tgcgacgatc aatccacgct gaccaaaacc gattggagaa aagatatcga tcattcgggt
     8401 agatagtctt tgcggtgttg tttccagttg aagctgtctt tgcggataaa gaggtgttaa
     8461 ggctggaaaa tgcggacgtt cttttgcttc ttccggatct tttccattaa cgctttccac
     8521 gtgcatcaaa ccataataac gttccgattc tttaggtgga cgggcttttc ctgccacttt
     8581 gtccccattt ctcaaactga atcgtctgat ttgtgaggca gaaatataga tatcttctgc
     8641 acttggtcca taattgattg gccgtaaaaa accgtaacca tcttgaccaa cgatatccaa
     8701 tattccttcc atataataaa atccttgttt ctctgcttgt gcacgaatca cagctagaga
     8761 taattccttt ttattcatct tactgtaata aggaatttta aattctttgg cataactata
     8821 aacttctttt aacgttttgt tctctagttc tgccattgtc aaataatcgc tcattcagct
     8881 cccacttcct ctatcatttc aattgatgcc cctaaggctg ttagtttctc gataatatga
     8941 tcataaccgc gtaagatata atctacattg taaatcgtcg ttgtcccttc tgccaatagt
     9001 ccagcaatca ctaaacaagc acctgcccga agatcagaag ccacgacttc tgtgccgtga
     9061 agtttatttg gaccattcac gatgatcata tttccttcca cggtaatatc agcgcccatt
     9121 cgtgctagtt ccggcacatg gttcacacgt ttcgcataga tcgtatcgat gatttctgaa
     9181 gtgcctgtag tcatcaatag taacgcagta agtggctgct gcagatctgt ggcaaatcct
     9241 ggataaggat aggtcatgat gtttgcgggt tttaattctt tcgaggggta aacttcgatt
     9301 tcatcttcgc tgattttcat atggacacca atttcctgca attttgctat aaagctttcc
     9361 aaatgttcaa agatcacatt tttcactttg ataccatttc ctactgcagc tgctaatgct
     9421 agataggttc ctgcttcgat gcggtcagga atcatgaaat gccgacagcc atgtaatgtt
     9481 tcgactcctt cgatacggat cacatctgta cccgctcctc gaacttttgc ccccatatta
     9541 ttcagtaaag ttgccacatc tatgatttcc ggttctcgtg cagcgttttc aattactgtc
     9601 tgtccttttg cttttacagc agctaacatc acattgatcg ttgctccgac tgaaaccacg
     9661 tccatgaaaa tccgattgcc acgcaatcct ttattttctg tccgtaaata catggcacca
     9721 tgttcatttg tcacttcagc acctagagct tcaaatcctt tgacgtgtag gtcaatcggt
     9781 ctagggccta aataacagcc acctggcagg cctactaccg cttcgccaaa tttaccaagc
     9841 agtgttccca taaaataata agaagcacgc aaactattga tttttccgtg cggcatcgga
     9901 atcgatacga cttctcgagg atcgatttct agtatgttgt tggaaaaatg gacagttgcg
     9961 cccatgatct ctaaaatttc aattaatgaa tgaacatctt gtatgtccgg cacaccttcc
    10021 aagatcactg gggaatcagc taagattgca gctggaatca atgcgactgc actatttttt
    10081 gcaccgctga tcgttacttc accagctaaa ggtcggtttc ctttgattac gatttttttc
    10141 atctgtttcc accttgttta ttaatctttt ttcatggata tgcttgtatt ttatcataga
    10201 taaaaaaaag aaacgagcat ggtctgccgt tttaagtaaa aaagaatgga gagcgacttc
    10261 aaaagtcgtt ctccattcct atatatcgta tctgttatgt ggatgcttac gctttgttag
    10321 ctgaaccgaa ccagtcgata tgagatttag catcttcaac gattgctgct ttacctggag
    10381 ctaataattt acgagggtcg aatcctttac cttctttatc tttaccagct tcgatatatt
    10441 tacgagtagc tgcagcaaat gataattgga attcagtgtt aacatttact tttgagatac
    10501 ccattgagat agctttttcg atttgatctt gaggaatacc tgatccgcca tgtaatacta
    10561 aaggcatgtc tggacctaca gcatcagcaa ttgcttgtaa gtggtcaaaa gctaatcctt
    10621 tccagttttc tggatattga ccatggatgt taccgatacc gcaagctaag tagtcgatac
    10681 ctgtagcaac catttgtttg cattcttcaa tatcagctaa ttcgccgtta ccgatgattc
    10741 cgtcttcttc tccgccgatt gaaccaactt cacattcaac tgagatacct tttgcatgtg
    10801 ctttttcaac aacgtcttta gctaatttca agttttcttc aaatggtaga tgtgaaccat
    10861 cgaacattac tgaagtatag cctaattcga tacattccaa agcagcttcg taatcaccat
    10921 ggtctaagtg gatagcaact ggaacagtga tgttcattgc ttcaactaaa tcagtgatta
    10981 aatctttaca gattttgtaa ccacccatgt atttagctgc acccattgat gtttggataa
    11041 gtactggtgc ttgttttgct tcagccgctt caaggattgc ttgagtccat tccaagttgt
    11101 ttgtgttgaa tccgccgaca gcgtaaccgc ctttacgagc agcttttaaa aattcagctc
    11161 ctgatactac tggcataata aaattcctcc taagttttgt aaaactaaat ttgttaagac
    11221 aattcttaac caacgaccct attttaccaa acatttcggt agttttctac taaaatcaat
    11281 gaaaatgaaa attttcaaga agttttttgc aaaaaataaa atatccaata gcgctttaat
    11341 cgaaaaaagt cttttgcttt ctatcttttt tcctacaaat aataacaaat cagaaaaaga
    11401 gaaaagtgac aagaaaattg cttatcccct tctcttgcta cttttctctt tctatcaagt
    11461 tcaaaaatta ttttgtttcg tgatctgcta atgatgctcc gacaaatgct ttgattaatt
    11521 tttgcgggcg atttggacga gaaatcaatt ctgggtggaa ttggcatccc acaaagaatt
    11581 ttttctctgg cagctctacg atttctacta aacggttatc tggtgagaca cctgaaaaga
    11641 ctagcccttg ttcttcaaac aattgacgat atttgttatt gaactcatag cggtgacggt
    11701 gacgttcttg cacaacatcg gctccatcat atgcttcagc cgtcttcgtt ccttttttga
    11761 ttttacatgg gtataatcca aggcgtaaag ttccacccaa attctcgatg ttttcctgat
    11821 cagccattaa atcaataata ttgtttgctg tatctggatt cgtttcagct gatgacgcat
    11881 cttctaatcc aacgacgtta cgtgcaaatt cgacacaagc catctgcata ccaagacaaa
    11941 tacctaaaaa cggaacgtca ttttcgcgag cataacgaat cgcttcgatt ttcccttcta
    12001 ctccgcgatc accaaatcca ccaggaacca agattccatc tgcgtctttc aaacgttctt
    12061 ctacattttc tctcgttaat tcttgtgctt tgatccaatc gatctcgatg tctgaattat
    12121 aggcgaaacc agaatgtttc aatgcttcaa ctactgagat atatgcatct ggtaactcga
    12181 catatttacc gacaagcgca atccgtactt ttttcttcaa gttcaagaca cgttcttcta
    12241 attctttcca ttctgtcatg tctgcttctg gtgtatctag ttttaaatgg tcgcaaacaa
    12301 tttgatccat tccttgtgct tgtaaaacta atgggatgga gtaaagtgta tctacatctg
    12361 gcgactcgat aactgcttct ggttcgacat cacaaaattg tgccaatttg tttttcacgc
    12421 tttgagaaac aggttgctct gtacgtacca ccaaaatatt cggttggata cctaaaccac
    12481 gtagctcttt tacactgtgc tgcgttggtt tagttttcat ttctccagca gctttcaagt
    12541 aaggaatcaa tgttgtatgg atatacataa cattatctgt accgacatct gctttcattt
    12601 gacgaagtgc ttcaaggaat ggcaaagatt ctatatcccc tactgttccc cctacttctg
    12661 taatgatcac atctgaatca gtcattttag ctgctcgcat gattttttct ttgatctcat
    12721 ttgtaatatg aggaataact tgaacagttg ctcccaagta ttcgccctta cgttctttac
    12781 gcaaaacttc agaatagatt tttcctgtcg tcacgttgga gtatttgttc aaattgatgt
    12841 caataaaacg ttcgtagtgg cccagatcca aatctgtttc agcaccatca tctgttacga
    12901 atacttctcc gtgctgatat gggctcatcg tccccggatc cacgttgatg tatggatcaa
    12961 atttttggat agttactttt aagccgcggt ttttcaataa gcgtcctaaa gaagctgcaa
    13021 caatcccttt tccgattgat gaaacgacac cgccggtcac aaaaatatat tttgtcataa
    13081 gttcaaaaac tcctttttaa tttatgtgca ggaaaagtct cagacaataa acaaagctcc
    13141 ctattcttca ataagaatag ggagccgaaa ttctgtaaac ttctgaccct ctaaaagggt
    13201 gcccaaggtg tataatacaa gctgactaag ggaaagtcaa gtactcaaat gaatactatc
    13261 cgcttacaat tttttctttt tcgatatcct ttgagaaatt atttcttaag tcctttatga
    13321 gtgcaaaaaa aatttaaccc acataataag cttgatataa acatagctaa cgttaaacct
    13381 aagcaaatca gaaaatcgtt actgaaaaat tttaagtgaa ataatagata aaaaaataaa
    13441 acaaacatta tgatagataa tatcccacct aacaaagaac tactttttcc tacttgcagc
    13501 aacagtttat atagcttatc gtagaaacaa tctttttctt tcacccttat ttacctcctt
    13561 tacgtaaaaa acgtagtcca cagttctcgg atttttggca tatcttttct attaaataat
    13621 atcaaaaaca aaacaattcc actaattttt tcaaagatga cctttttttc aacaaaagtg
    13681 ccttgtaggg attcattgaa ggtaacgaat cacataagta aaaaataagg agatgcgaca
    13741 aaa
//
LOCUS       DACECR010000075        13705 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.075, whole genome shotgun sequence.
ACCESSION   DACECR010000075 DACECR010000000
VERSION     DACECR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.075"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(18..710)
                     /locus_tag="IWO17_002047"
     CDS             complement(18..710)
                     /locus_tag="IWO17_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0290910.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            705..971
                     /locus_tag="IWO17_002048"
     CDS             705..971
                     /locus_tag="IWO17_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0290911.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1147..1716)
                     /locus_tag="IWO17_002049"
     CDS             complement(1147..1716)
                     /locus_tag="IWO17_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0290912.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..2113)
                     /locus_tag="IWO17_002050"
     CDS             complement(1790..2113)
                     /locus_tag="IWO17_002050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290913.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVITDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2267..3079)
                     /locus_tag="IWO17_002051"
     CDS             complement(2267..3079)
                     /locus_tag="IWO17_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290914.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3433..4281
                     /locus_tag="IWO17_002052"
     CDS             3433..4281
                     /locus_tag="IWO17_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ0290915.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPRPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4426..5367
                     /locus_tag="IWO17_002053"
     CDS             4426..5367
                     /locus_tag="IWO17_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ0290916.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLKKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFTHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5357..7528
                     /locus_tag="IWO17_002054"
                     /pseudo
     CDS             5357..7528
                     /locus_tag="IWO17_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7640..9811)
                     /gene="copB"
                     /locus_tag="IWO17_002055"
     CDS             complement(7640..9811)
                     /gene="copB"
                     /locus_tag="IWO17_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ0290917.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9831..12017)
                     /locus_tag="IWO17_002056"
     CDS             complement(9831..12017)
                     /locus_tag="IWO17_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ0290918.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="IWO17_002057"
     CDS             complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="IWO17_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ0290919.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12239..12679)
                     /locus_tag="IWO17_002058"
     CDS             complement(12239..12679)
                     /locus_tag="IWO17_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ0290920.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12753..13292)
                     /locus_tag="IWO17_002059"
     CDS             complement(12753..13292)
                     /locus_tag="IWO17_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0290921.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3850 a         2877 c         2440 g         4538 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttc
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctctcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagtcat tcttggtaaa
     2281 gtacttttgt cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagca aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattca atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgggttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg
     3301 aaataaatag tgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgagaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacttct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct agacttgtat
     5041 ttttctttat ggcatttgaa aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcacacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggtttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagttacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattta ttacattgac cgtggattac gactattggt acgcaccctt
     5761 tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta
     5821 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     5881 catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     5941 tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga
     6001 ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg acacactcca
     6061 tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg
     6121 tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaagaag gaaatggcta
     6181 ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc
     6241 acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttataga
     6301 agacaaagaa aacattacat tgaccctaaa aaaacaagtt ttcttacctt atccttatgg
     6361 aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga
     6421 aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga
     6481 aaggttagtt tttacgagag aagaattaaa aatcagtcag ttaaaagctg atgatttaga
     6541 aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc
     6601 ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tctaacagta
     6661 tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata ctccaaaaga
     6721 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
     6781 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
     6841 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
     6901 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
     6961 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
     7021 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
     7081 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
     7141 cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa
     7201 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
     7261 tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc
     7321 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
     7381 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga
     7441 taaaaaaacc tatttagaaa taacgcatat aataagaaaa atggtataca aaaaaattct
     7501 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat
     7561 gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg
     7621 tcacaacctt ttttcattat tatctgattt tcaatgtcaa agcatttaga gctacaacta
     7681 ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata ccaattggcg
     7741 caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc caccagaggt
     7801 tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg
     7861 gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc cctgcaccga
     7921 ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca tcacccacca
     7981 tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta tcatctggta
     8041 ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg gcagcgtttt
     8101 tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt cggataaatt
     8161 cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga tgattttcta
     8221 aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat gcttgttcta
     8281 cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt tgttttttga
     8341 ctgttgcctc tagcccgact cctgaaagat tttttaggtt ttcagcttga taaggcttga
     8401 tttcatgttc tttcaaataa ttcataatgc caacagccaa tggatgattg gcattcgctt
     8461 ccaaggctcc tagatattga aggatttcat ttttattgaa cgcatcatca agtacttcga
     8521 cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc atgatcacat
     8581 ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg tttttagctg
     8641 ctattgatgt ggaacgagca acaactaatg ggatagctaa acctagagca tgaggacagg
     8701 cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc aagaacaacc
     8761 aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat tttgccactt
     8821 tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc atttccataa
     8881 ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc gttccatccc
     8941 cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct cttgattcgc
     9001 cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca tctgttggca
     9061 ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc aatgaaactg
     9121 tctcttcttt tccatctttc gtcaagcgtt tcactgattc tggcagtaat tctgctagtt
     9181 tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga cctagtagca
     9241 taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta tgtggattga
     9301 gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc cccatcgcga
     9361 tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca cttaaaaatg
     9421 gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa tcagaaccag
     9481 gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga atgattggga
     9541 tagctaggat aagcgatagc caaaattttt gtttgaaatt ccccatgtgc atggaatgat
     9601 ccatgccgcc gtgttcgtga tccatgtgca tcgaatggtc catttgtttg ctgtttgcta
     9661 tatctccatg gttatgtccc atgtgcatgg aatgatccat ctgactatgg tcatgtttca
     9721 tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct tttttatgct
     9781 catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc ttactttttc
     9841 atgtgtcgat ttaagctcag ggagttcaac aatacactga cggaactaaa cgccatcgct
     9901 cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc aatcgtatta
     9961 tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaacct agacaactcg
    10021 attgtttgtg caatggatgc caaactgcta ttcatcagcg tcacatcggc tgtttccatc
    10081 gcaatgtccg ttccacttcc cattgccata ccgacatcag ctaatgctaa ggccggtgca
    10141 tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag tttttctacg
    10201 taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc aataccaact
    10261 tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa aacatctaat
    10321 cctttattct ggagctgctt gattgcttgt ttgacttcta gtttgatttg atcagccacg
    10381 gcaatcaacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt tccctgctgt
    10441 tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc aattaatcgt
    10501 ttacgtgtac ccgcaaaata gcgtactccg ttaatcgtac cgctgattcc agcacctggg
    10561 tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc gtattccacg
    10621 attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat agataggact
    10681 tctttagaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt tccagtttta
    10741 tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc tttaattaaa
    10801 attccatttc gtgcaccaac acccgttcct accataatag ctgttggagt cgctaaacct
    10861 aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc gagttgccaa
    10921 tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa tacgatcgga
    10981 acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct cccttgagca
    11041 tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg tgtaacttcc
    11101 atgtgaagta gaccattcgt attgattgtc ccgccaaaga gcgtgtcatc cggattcttt
    11161 tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct tcctgaaata
    11221 attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc accaacgact
    11281 acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc ggtttttgtt
    11341 tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt atgttccaaa
    11401 tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata aagatcttgg
    11461 gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt
    11521 gctaccaata catccatatt tggtgctttc gtcttgattg catggtacgc acctttataa
    11581 aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac gattggaaaa
    11641 tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat tgacaacatc
    11701 aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt caaatacgct
    11761 tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc accatagccg
    11821 atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt gacacttgct
    11881 ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt cagctctttt
    11941 tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt atccattttg
    12001 gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg attgcttgac
    12061 aaatcttttc ggattgaact tttgtttcat caaacttgac cgtaccgttt gcttttttca
    12121 aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct actcttgctg
    12181 cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt tcttcctcct
    12241 attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc gtatctactg
    12301 gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc aaagtggctt
    12361 cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg gaaaaaagat
    12421 tttccgtcgc actgcgaact gtttccatct ccgagacagc tggatgataa atgaattttt
    12481 ttccttcttg ttctgtccag atcacttctt tttttaccaa acgacccagc aaagttttga
    12541 tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct tgggctgtcg
    12601 tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcactg atctttactg
    12661 gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat cattatctta
    12721 tgaatacaga ttacacttgt aaacaaaaac tgtcaattat ttttgctctc tattttcaat
    12781 ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt atttcttgca
    12841 ttagctactt ggatcagctg ctgtattttc ggttcattgt tttcttcaaa atgccagata
    12901 aaagataaaa attctaagta ttccctgttt agtttctccg aaaatgattc aggcatatct
    12961 ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg gtagatggcg
    13021 agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct ttcctctaat
    13081 gtagaagtat aatttccttc aacgatccat gacggattag ataccataaa caattgctgt
    13141 tcttgcaaaa accacttttt tgcctgaata ctgtagtctg ttgtatgcca taaaaggtct
    13201 agcggcagta gcggaaagtc ggtgatttct ttgatcttcc ttgcgagggt cgattttccc
    13261 gacccacttt gtccaatcac gatgatcttc attcttctcc ctcacttctc tttggaattt
    13321 ggttcgatca taccacttat aacgccgcaa aaaaacaagg gactgtgaca aaagtcgtgt
    13381 caaagtccct tgtttcgaat aaatggtgtt caatcagctg accttcagca ttagctcaaa
    13441 aagcggcaaa atatgaggag ctatttttcc gtttgtttct ctaatactca ggtcaaatcg
    13501 ctgattttac aacctcttcc ttaaacggtg ttctcctgcc aacacctcgg tcacaagtca
    13561 caatagtcaa caaacatgag aagttgtttt ttgaaattgc tccttgtgac tcggagttaa
    13621 acggcaggct cactacctct ttcttaaacg gtgttccaaa gcttgcatct gcggtaataa
    13681 gcccagccaa acgaaaaata tgagg
//
LOCUS       DACECR010000076        13546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.076, whole genome shotgun sequence.
ACCESSION   DACECR010000076 DACECR010000000
VERSION     DACECR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.076"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(74..1030)
                     /locus_tag="IWO17_002060"
     CDS             complement(74..1030)
                     /locus_tag="IWO17_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ0290922.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1134..1736
                     /locus_tag="IWO17_002061"
     CDS             1134..1736
                     /locus_tag="IWO17_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0290923.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1824..3323)
                     /locus_tag="IWO17_002062"
     CDS             complement(1824..3323)
                     /locus_tag="IWO17_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ0290924.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3339..3863)
                     /locus_tag="IWO17_002063"
     CDS             complement(3339..3863)
                     /locus_tag="IWO17_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290925.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4161..4910
                     /locus_tag="IWO17_002064"
     CDS             4161..4910
                     /locus_tag="IWO17_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ0290926.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4901..5686
                     /locus_tag="IWO17_002065"
     CDS             4901..5686
                     /locus_tag="IWO17_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0290927.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5655..6371
                     /locus_tag="IWO17_002066"
     CDS             5655..6371
                     /locus_tag="IWO17_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0290928.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6473..7492)
                     /locus_tag="IWO17_002067"
     CDS             complement(6473..7492)
                     /locus_tag="IWO17_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ0290929.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7511..8569)
                     /locus_tag="IWO17_002068"
     CDS             complement(7511..8569)
                     /locus_tag="IWO17_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ0290930.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(8790..9938)
                     /gene="nagA"
                     /locus_tag="IWO17_002069"
     CDS             complement(8790..9938)
                     /gene="nagA"
                     /locus_tag="IWO17_002069"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ0290931.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(10014..10850)
                     /locus_tag="IWO17_002070"
     CDS             complement(10014..10850)
                     /locus_tag="IWO17_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0290932.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(11034..12251)
                     /locus_tag="IWO17_002071"
     CDS             complement(11034..12251)
                     /locus_tag="IWO17_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0290933.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            12634..12717
                     /locus_tag="IWO17_002072"
     tRNA            12634..12717
                     /locus_tag="IWO17_002072"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12668..12670,aa:Leu,seq:gag)
     gene            complement(12957..>13546)
                     /locus_tag="IWO17_002073"
     CDS             complement(12957..>13546)
                     /locus_tag="IWO17_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290934.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         4097 a         2767 c         2329 g         4353 t
ORIGIN      
        1 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
       61 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
      121 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
      181 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
      241 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
      301 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
      361 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
      421 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
      481 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
      541 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
      601 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
      661 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
      721 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
      781 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
      841 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
      901 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
      961 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
     1021 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
     1081 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
     1141 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
     1201 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
     1261 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
     1321 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
     1381 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
     1441 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
     1501 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
     1561 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
     1621 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
     1681 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
     1741 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
     1801 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
     1861 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
     1921 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
     1981 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
     2041 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
     2101 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
     2161 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
     2221 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
     2281 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
     2341 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
     2401 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
     2461 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
     2521 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
     2581 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
     2641 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
     2701 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
     2761 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
     2821 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
     2881 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
     2941 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
     3001 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
     3061 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
     3121 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
     3181 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
     3241 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
     3301 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
     3361 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
     3421 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
     3481 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
     3541 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
     3601 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
     3661 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
     3721 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
     3781 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
     3841 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
     3901 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
     3961 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
     4021 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
     4081 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
     4141 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
     4201 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
     4261 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
     4321 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
     4381 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
     4441 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
     4501 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
     4561 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
     4621 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
     4681 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
     4741 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
     4801 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
     4861 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
     4921 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
     4981 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
     5041 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
     5101 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
     5161 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
     5221 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
     5281 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
     5341 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
     5401 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
     5461 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
     5521 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
     5581 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
     5641 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
     5701 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
     5761 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
     5821 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
     5881 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
     5941 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
     6001 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
     6061 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
     6121 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
     6181 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
     6241 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
     6301 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
     6361 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
     6421 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
     6481 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
     6541 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
     6601 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
     6661 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
     6721 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
     6781 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
     6841 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
     6901 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
     6961 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
     7021 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
     7081 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
     7141 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
     7201 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
     7261 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
     7321 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
     7381 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
     7441 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
     7501 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
     7561 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
     7621 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
     7681 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
     7741 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
     7801 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
     7861 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga aatagccacg
     7921 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
     7981 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
     8041 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
     8101 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
     8161 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
     8221 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
     8281 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
     8341 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
     8401 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
     8461 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
     8521 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
     8581 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
     8641 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
     8701 aacttatgta aagtgatttt tactagaaca caaaaaaaac cttaaaacca aacttaagaa
     8761 gtttagtttt aaggttaact taaattatat tatgcgtgga aacgttccac gccatctaga
     8821 taagttgctt ccaatgtcat atcagggttc aatacgataa agtctgcatc gcgtccttga
     8881 gcgatttttc cacatgtatc atcgatttta cagctgattg ctggaatcag actagccatc
     8941 atgactgctt gttctggtgt tgctaatccc cagtctacaa catttttaat tgcttctttc
     9001 aatttcaata tacttccagc taagttgcca gattctagac gtgctgttcc ttcttttact
     9061 actactggaa attcgcctaa attgtaattt ccatcaggca tcccaccggc catcatacag
     9121 tctgtaatta atgcgacatg atcatgtcct gctttttcca ttaggattcc cgcagcattt
     9181 ggatgtacat gatggccatc acaaatcaat tcagaaaaga catgttctaa agataataac
     9241 gcacccacca tacctggttc gcgatgattc aatccacgca taccattgaa agcatggaca
     9301 aatacacttg cgcctgcttc aacagctttt tgcgcttctt ctagtgtagc attactgtgt
     9361 cctagagaaa caactactcc ttcttcagtc acttgtttga caaactcttc tacaccttga
     9421 cgttccggtg ctaaagcaat ttttttgatt aaaccacctg aagcttcttg ccattcatgg
     9481 aaagtatcca aatcaggatc cccgaaataa cttgggtttt gtgccccttt gtgttcttct
     9541 gtgaagaaag gaccttcaaa atagattcct ttgattttag ctcctggtgc ttcctgatac
     9601 attttgccga ttgtctcagc tacatctttt aaacgttctt tgcttgacgt caatgttgtt
     9661 ggtaaaaatg aagtcactcc acaagataac agaccttcag acatcacttt gatgccttct
     9721 gcatcattat ccattacatc atgattcata tagccgtgaa tatgtgtatc taccaaacca
     9781 ggagcaatcc atttgccgct ttggtcaatg atgttgactt ctccctcagg tagttctttg
     9841 gtgtattcac caaaaagtcc atcggtaatt tcaagatagc ctgcatgttt gacacctgac
     9901 gctaaaaaga atttgtcagc atagataaaa gttctcattc tattctctcc ctttgttttt
     9961 ctacctctaa agtactctta tttagagaag aagtcaagaa tggtctacac catttacaaa
    10021 ttttttgaaa tttgtttcac agcttgtgct aagccttcat agaatgctgc tctttctggt
    10081 tcttctttac gcttgtatat taagctttta agcacaaata aatcattgag gtaatagtag
    10141 ctattcttct cttgagccca atagattcct tgattcacct gttctaacgc ttcgtcgatt
    10201 tcattttgat ggaataagaa atttccataa ttataaaaaa tttgcgaaaa ttcacttttt
    10261 gatcgttcat cagctgattg atgcagcagt tggatgcttc gactcaaata gtagcgtgct
    10321 tcagccgttt ttcctgccac accatatatt tttccgatac aactaatcag ttgtatttct
    10381 gtgtcagaaa catggattct ttctttttgg ctagagtagc ttaatgcttc tttcaaataa
    10441 taaagggact gagagatatt ttgacgcaac gtgaattcgc aaacaccgag ataataataa
    10501 tagcgttgaa agtcagttgc ttgatacaga ttttcagaaa tatcagaaga ttttagtact
    10561 tgttctaact tccgataatc acgtttttgg aaacaactat ccaacagttc aaataattta
    10621 tcgtttgtcc gagtagtttt tcgagagaga ttcatcacat gatcaatcgt tacccctagc
    10681 cgttgacaaa tctgcatcat caccaataca ttcggcagtt cctcattatt ttcgatccga
    10741 cttaatacac tttgtgaaca aatatcatgc gaaagcattt tttgcgtcat tttttgttct
    10801 tttcgaatct ctttaatgat cgttccaaag gactcgatta acgtttccat aagtatcctc
    10861 cccaaaatat gcgtattcgc attctattcg tattttactt taggaagtcg caaacttcaa
    10921 gaaaagactt gcaactaaat aatatacttt agttatttaa gaaaactcca ctagaaagag
    10981 agcctcagtg tgtttgtctg tggccctcct actgaaaaat ttggttttta aaatcatttt
    11041 tggcgatagg ttgctaggaa ttctctcatt ttcttagctg tttgtttcaa atcatccatt
    11101 ttaggaagat aaacgattcg aaaatgatct ggctggttcc aattaaatcc accaccatgt
    11161 acaagtagaa tatgatgttc atgtaaaaag tccaaaacga atttttcatc atccaaaata
    11221 ttgaatcgct ttgtatcaat ttttgggaat atataaaaag cagcttttgg ttttacggct
    11281 gaaagaccag gaatatcatt gattgcatta tagatatatt ctctttgttc atagatcctt
    11341 ccgcctggca ggagcagctc atcgacactt tgataaccgc ctagcgcggt ttggataatt
    11401 tgctgagaca atacattgga acacaaacgc atggaggaca acatattcag cccttctata
    11461 tagcctttga cgtgtgactt atctccactt aggaccatcc agcctactcg aaatcctgct
    11521 acacgatgag atttggatag accgttcagc gtaacaacga acaaatcagg cgctaatgta
    11581 gcaatcggaa tatgttctag accatccatc actaagcgat catagatttc gtcggaataa
    11641 ataatcaggt tattttgtcg agctacttca acgatttctt ctaataattc ttttggataa
    11701 agtgccccag taggattatt aggattaatg ataacaatag cttttgtccg actcgttatt
    11761 tttgatttga tatcatcaat atcaggatac cattctgcct cttcatcgca aatataatga
    11821 acaggtgtgc ctccagctaa tgcaatagaa gccgtccaca acggatagtc cggcattgga
    11881 accagtactt catctccatt gtccagcaac ccctgcatac acatcgtgat caattcgctc
    11941 actccgttgc cggtgtagat atcattgatc gtcacattcg gaaaatgttt cagttggcaa
    12001 tattgctcaa ttgcttttct agcagaaaag atccccttgg aatctgagta gccttctgag
    12061 tcacgggcat tcatgatcaa atcacgaatg acttcgtttg gtgcttcaaa tccaaaaggt
    12121 gcgggatttc ccgtgttcaa ttttaaaata ctaatgccct cttcttgcat ccgctccgct
    12181 tcttctagaa cgggaccgcg aacatcgtaa ctgacacctt ctaatttgtt ggatttttca
    12241 aattctctca tcacttagcc aatccctcat ttcaaaaagt tagaactttc caaatttttt
    12301 gttatttact atttcaatat catactccat cctagaaaat tttgccactt atttaggaaa
    12361 aacgtaagta ttaaaaaaac acaaacaagc gttgagaact taattgtcat gatagtcatt
    12421 ccatagattc gctgcccaat ccattgcaat tccacaaatg attcctagca taaaaccaag
    12481 caaaaaacgt ccgaatgcaa gtccaatcaa tacacctaag atcattccca gtcctgaaaa
    12541 ttctccgtca tatttctcca tcttaataag tcctcccatt tttgagcttg acaactatgt
    12601 aaaacctcgc tatcattagt gtgttattat tacgcagatg tgttggaatt ggcagacaag
    12661 catgattgag ggtcatgtga gcgttagctc gtgtgggttc gagtcccatc atctgcacta
    12721 caactcgtga agttaatttt gcttcacgag tttttttgtt tgaacgtttc tttatgccat
    12781 tatatacata tcactgtatc ctttacaaaa agaacgtaca atcatgtaaa cagcctgagg
    12841 tgagtatatg aaggtcggaa acaagctact cgtagaaact tgcaagtgtt aaaacaatta
    12901 atcgaaaaac gagtaaaatc cctcacctaa tactaaagcg agggattttt tgtatattat
    12961 ctgtttatca ccgttattat aaaggtacaa ataccaagca cggttaatcc aaattgcata
    13021 aattcatttt tttgtacact catgcttttc attagctctt taactgccat tagtactaag
    13081 atggcgataa caacataaag tatatagtac gtccgcattc cggaagccca tactgtggga
    13141 gaaaatccca taattaacct tgaacaaaat cccattatca tcaaaataac taaaaaaata
    13201 gaagttttct tattgtcaaa acttaaataa attccaacaa ttaaacatag taataatgcc
    13261 aagactacta aagtagctat ccaagtacct ggataaaaaa gtgacagttt cgttcctaac
    13321 tctgtaaaga gattattcag gttactacta ctccaaatca ttgccctttt atttccattg
    13381 acgtaagtaa agctttgacc cattgtatta ccaaaataaa taatcaggtt taagaaaaac
    13441 ggtaatgcag tcaatatacg cacataatag ttttgacatt tcctatatgt tagaaggaaa
    13501 attaagaaaa atagcaataa aaataagata ttcatgtcta aaaaaa
//
LOCUS       DACECR010000077        13344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.077, whole genome shotgun sequence.
ACCESSION   DACECR010000077 DACECR010000000
VERSION     DACECR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.077"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(90..533)
                     /locus_tag="IWO17_002074"
     CDS             complement(90..533)
                     /locus_tag="IWO17_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0290935.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(728..2068)
                     /locus_tag="IWO17_002075"
     CDS             complement(728..2068)
                     /locus_tag="IWO17_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0290936.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2350..3246
                     /locus_tag="IWO17_002076"
     CDS             2350..3246
                     /locus_tag="IWO17_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0290937.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(3389..4330)
                     /locus_tag="IWO17_002077"
     CDS             complement(3389..4330)
                     /locus_tag="IWO17_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0290938.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4456..5577
                     /locus_tag="IWO17_002078"
     CDS             4456..5577
                     /locus_tag="IWO17_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0290939.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            5835..7337
                     /gene="lsa"
                     /locus_tag="IWO17_002079"
     CDS             5835..7337
                     /gene="lsa"
                     /locus_tag="IWO17_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0290940.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            7404..7931
                     /locus_tag="IWO17_002080"
     CDS             7404..7931
                     /locus_tag="IWO17_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0290941.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            8143..9204
                     /locus_tag="IWO17_002081"
     CDS             8143..9204
                     /locus_tag="IWO17_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0290942.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9207..10103
                     /gene="murQ"
                     /locus_tag="IWO17_002082"
     CDS             9207..10103
                     /gene="murQ"
                     /locus_tag="IWO17_002082"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0290943.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            10117..12099
                     /locus_tag="IWO17_002083"
     CDS             10117..12099
                     /locus_tag="IWO17_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0290944.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12169..12615)
                     /gene="nrdI"
                     /locus_tag="IWO17_002084"
     CDS             complement(12169..12615)
                     /gene="nrdI"
                     /locus_tag="IWO17_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0290945.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            12826..>13344
                     /locus_tag="IWO17_002085"
     CDS             12826..>13344
                     /locus_tag="IWO17_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0290946.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVA"
BASE COUNT         4354 a         2418 c         2804 g         3768 t
ORIGIN      
        1 attcacggtg ttccagaagt agcttcttct tatttcttga agttgatcac ttctggaaca
       61 tcctcttttt ttatttggat ggataatggt cattccattt ctttgatcat ttttttcatt
      121 ttgtcgattt cttcttcttg tgcctgaatg atttcttccg ctaatttttt tacttcagga
      181 tcttttaaat cagctcgttt acttgtcaag atagcaatag agtgatgagg gatcatcgcc
      241 ttcatccacg ccgtatcgtt gacagttgtt tggcttcgta ccataaataa ggaaccggca
      301 aaaacaagaa gactagcggc taaaatcata gcatttatct ttttgttttc atacattttc
      361 cacatgaaca acaacatgat gactgccatg actgcaccca tcatcaacgc catgaataca
      421 cgtgtgcgac tgaagaagat atgatcaagc gcataaacat tcaaatacat cataccaaac
      481 atgactaccg tcgaaacacc aatcatcaac aaaaacttcc aatacttttt catttaaaac
      541 aaactccttt ttcttcattc ctttcttcta tcatgtcatg agcgtggaga acaatcaatt
      601 aatctgcaca gatggttttt gaacaccgtt tattcggaaa aaagaactgc aacaattttt
      661 gtcgcagctc ctttttttca atagctccag atattcaata taaatagaat aaaattctct
      721 attggtttca ttcattcgtc aagacgaaag catcattctt tttttgataa ctgaaataaa
      781 tgaacaggta aagcaaaccg gtcaatacag ttaccccaat ggagttcgtg aataggatac
      841 caaggaaaat caaccataag actgctaatg caaaaaagct gattcctttg tcccataacg
      901 ttcctttcct tagtgtcagt acaaaggacg cagcactgat aatgatccag atcaataata
      961 ctgtatagga aagagaacct gccagatagc caaataattt gtcaccgaca aaataagaaa
     1021 gagccacacc tagataaaga acactggtac aaaacagcac agcacgttgc ggtacttgat
     1081 gcttgtttaa aacagctagt tttctagagg aacctttccg atctttcaag cggaagaaca
     1141 aaattctcga agaagcatat acacctgaat tgatagacga gaacagagca agaacaatca
     1201 cgaaattgac gatatctgcc gcaaatggaa tattcatttt ttcaaatacc atcacaaaag
     1261 gactaacgtt cgtgcctgct aaagtattcc aaggaaaaat aatcaaaagc aaaaataaag
     1321 gaataatgta aaaggaaatg attcttccca tgacgccttt gattgcttta ggaatcgcac
     1381 gtttaggatc atctgcttcg cttactgtga tagcaatcaa ctcagaaccg ccgtaagaat
     1441 agataactac aagcaaagaa ctgatgattc ccttcatacc gtggggagta aaaccaccat
     1501 gatcagttaa ttgttgaagg gtagggaaaa caccagtacc caacatttgc tgaccgacaa
     1561 gataagcagc aaaaatgatc aataggataa tgacactgat tttagcaaag gccagccaat
     1621 attctgtctc tgcgaataat cgtaccgaat aaagattgat gatagtagtt aaaacagcca
     1681 acataaaaac aaatacccaa gctgggatgt gggggaacca caactgtaaa aaggaagcag
     1741 ctgcaacagc ttcagcaatg atattgataa tccacatgga ccagtagacc caatcggtaa
     1801 aatcggcagc atgagcacct agatatggac gaatcaatcc agataatccg tggtgcgtat
     1861 caccaccact taaaacaatt tttcccagac ctttcatcac gatatataaa attatgccgc
     1921 caatcaaaaa agcgatcaat acagaagggc cggcgattcc aatcgcttcg ccacttcctt
     1981 taaatagacc tgcaccaatc gctccaccta aagctagcat ggtgatatgt cgggagctaa
     2041 gttgtttttt caatttttta tcttccataa ataacctctt tcttttaaat tcacaatcaa
     2101 aatgcatagt aagaataaat agtaagaata aatagtaata ataataatga ctaatgataa
     2161 caaattttca gataattaca actattcaga aaacttttgg gcatatttta atggaagaat
     2221 gatttatttc atcgtatttt catagaaatg ttaaattttt ataaaaataa taggaagcgt
     2281 tttactggct ttttactatt taaacaggct atcataggaa aaagtgacag agaaagtgag
     2341 gcggaacaga tgaacaaaaa cgacgtattg ctaattgcga aaaataaatc actttatttt
     2401 tttccttcca tccgctcttt agatgtttgg tccggaaaag atttactaca gtcggaattc
     2461 attaaatcag ctcgacaaca gttggcaaat tatcagctga tcgttttttt agattatgga
     2521 tttgatatgg agatggctcg acgcgtgcgt ccatttacca atgcaaaaat cgtgttattt
     2581 ttttggaacc actttaaagc agaacacgag gcgatgctga agcaggcaca agatgaaccg
     2641 gcaatcgatg agatttatca ttttgatttt ttagaagcga aagaacttgg actaaaacat
     2701 aatagttcct tttattcgaa acatatggat ttttcttcta gcgaaccaaa gacggatcta
     2761 ttttttggtg caacaaataa cggcagaaaa gaacgggcag aaaaatataa gcaggaattt
     2821 acaaaaagag gtgttacgac caattacttt attcttccgc ttcgaggaaa tgaacaacct
     2881 ggatacctta cttatgaaga atatctgaaa aaaaccgggg aatcgagggg gattctagaa
     2941 ctgttgcggg aagggcagca aggagtgact ttgcgaacgt ttgaatctat gtattttcaa
     3001 aagaaactag tgacagataa tcaagccata gctcattatc agttttatca ttctgaaaat
     3061 atttttttgc ttcaagaacg tgatttagat gaattaccgg aatttttgca tacgccattt
     3121 caaccaatcc catcagaact gttggaattt tttgatgcag aaaattgggc aaaacgtttt
     3181 ttcactactg actccgctat ttatgaacaa tatgaatacg atctttcctc tgttaaaaat
     3241 aaataaaagt gccaaccaat catagatgaa aataaacaag cgcaattctt atggcaacat
     3301 gagaattgtg tttttctttt tgaactttca tgggggtaaa aaaatgagaa ccagctgctg
     3361 atccagctga ttctcatcag agagcctttt attctgcgcc aaggattcct tcttttgcat
     3421 ggactaattg ggtcaaaaag gcacaatatg gtgtagcaat atcatatttt tgtccttttc
     3481 gccaaacggc accattgatg tagtcgattt cagtcaaacg atggtttttg atcaggtctt
     3541 ggtacataga tgggtaatgc aacccgatgt tgtcaggatc ataagtagaa gcaatatgtt
     3601 ggtacacttc ttcttgatct aatgtgattc cttcttttgc tgcgatagaa gcaaactcag
     3661 atacaatcgt tcgaaccatg ccctctgccg ctttttgcgc acctaattca gcaatattac
     3721 aatccaatat cgtgcataat ccatttaatg ttccgtttac acaagctttt cgccaaatgg
     3781 aataacgaac attatgacta tagattggat tcaattttgc ttcttgaaag acttctacaa
     3841 ctttttttgt aaatgcttcg ccttctggtt ccaagttctc tagttcgatt tctccatcac
     3901 ctaataatgt gacttttccg ggaccagcta gaccagctgt ccacatcgtg atcccaaata
     3961 agatattttt ctttggtaca tatttttcca gtacatcttc atgacctaat ccattcagta
     4021 aacacaaaac ataagtattc tcagtaataa tggattggat cgaacgaaac atatcatcta
     4081 attgattcgc ttttgtcaag gctacgatca aatccacgtg tttgttgctt tcgatgattt
     4141 cttctggtga atagattggc agttttgcaa ctacttcttt gccattaaag tcagcaatca
     4201 atccatttgt acgaatggct tcaatgtgag caggccagcg atcgatcaag acgacctcat
     4261 ttccgctttg atgcaacatt aatccgattc ggctgcccat tgctccggct ccagctattg
     4321 cgattttcat gttttttctc tcctttattc aatgagttcc cttttagtat atagaaaaac
     4381 agaagaaaat agcattgttt tgtttgaaaa aagtgaaaaa aagtgtctta cttaagaaga
     4441 aagtgaggga tatagatgga tattttggcg gcaatcgatt catttaaagg aagtgcttct
     4501 tcatttgaat taaatcaggc agttctaaat ggttgggaag accgtaaagg aagaaaaatc
     4561 aatgtgccga ttgcagatgg cggagaaaat actgcagaag caatctatta cgcgctcggg
     4621 ggaacctggc gtacgattga tgggatggat ctcttatttc gtcagcgaaa aatcagttat
     4681 ttattgacga actggcaagg aaaaaatacc gcagtcattg aatctgcaca agtattagga
     4741 ttagatttac ttgataaacc aacggatcaa accatccgac ttgcttctag ttatgccttg
     4801 ggagagctta tcaaagatac actgcttcaa gaagtggaac aaatcattgt cactttagga
     4861 ggaagcggat gtagcgatgg tggtctagga ttactccaat ctttgggtgc ttctttagaa
     4921 gggtacacag aaggaaatcc tttgttatct actagaaaac ttcatttgga tcgaaggaac
     4981 gttcttgttt ctaaagtaaa agtgatcatc gctgcagacg tgaccaaccc atatacaggt
     5041 caacaaggag ctgcttggca atttggaaga caaaaaggag gaacggatac gacgttagct
     5101 ttcttggata aacaagctga aaagattgct cagcagataa aagatgtttg ggaggtggat
     5161 ttagatgaca tcccaggaag cggagcagca ggcggtattg gcggagccct ttttttgtta
     5221 ggagcgaaga tggtctctgg ttttgagttg gtcagccaaa tcactggatt agaaaaacag
     5281 atcgaacaag cggatgtcat cattacagga gaagggcgga ttgatcagca aacaatccac
     5341 ggaaaagttc catatggcgt agcagtgctt gcaaaaaaat atcgaaaacc aataatcgct
     5401 atttgcggaa gtagagaccg agagctggaa ggactctttg attttctccc cattgtctta
     5461 agcattcaat taggaccaat cgatttagag gaagcaatgg aacacaggca aacattagaa
     5521 aaaacagaaa tcttaggaca gacactcagc agatttttca acattctccc gaagtagttg
     5581 acaaaaacaa tttctttatg ctatcgtaat ttcaaatata ggacaaagtg agggatccgt
     5641 gcattgaaaa attaagtcga ttgatgaaag ctgcattcag actgtacctt gtggagacaa
     5701 gggagcagtg tgcagatttt cgggattggc ttggttcttt cgatgtatgg gtctttttgt
     5761 gtgccccaac ccttgcatcc tctttagcca gttctgaaaa taggactggt ctttttttac
     5821 gaggtgatgg aataatgtct aaaatcgaaa taaaaaatct gacattcggc tatgacagcc
     5881 aaggcacatt attatttgaa caagccaatc taaactttga cacacaatgg aaactaggac
     5941 ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tatcctgcaa aacaaactcc
     6001 cttatcaagg gcaagtgatc catcagcaag cgtttgtcta tttcccgcag caaacaaaag
     6061 ataaagagcg tttgacctat tatgtgttaa atgagattat ggattttgag atatgggaaa
     6121 ttgaaagaga gctccaactg atgcaaacag atcctgaaat cttatggaga gaattcagca
     6181 cactatcagg aggagagaag acaaaagtcc tactggcact cttatttgtg gatgacactc
     6241 atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggcaga aaacaagtag
     6301 cggcttactt gaaaaagaaa aaacaaggat tcatcgtggt gagtcatgat cggggattta
     6361 tcgatgaagt agtggaccat gttttagcaa tcgaaaagag ccaactggaa ctttatcaag
     6421 ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa atagctaaaa
     6481 atgaaaaatt gaaaaaagaa gtcagcaggc taaagaaaac agcagctgaa aaagcagaat
     6541 ggtctcgttc ccgagaaggg gacaaaacaa agaaaaaagt cggattcatc gatacggaat
     6601 ctaggcgagt gaataaagga gcagtaggcg ctgatgctgc acggacgatg aaacgatcta
     6661 aagcaatcgt gaatcggatg gaaacccaga tcagtgagaa agaaaaatta ttaaaagata
     6721 tcgagtatat cgatgctttg acgatgaata gccaagtgtc tcatcataaa cggctgttaa
     6781 gcgtagaaaa tcttcaatta gggtatgaaa atctgttatt cgagccagtt cattttacga
     6841 tcgaacctca tcagcggatg gcaatttcag gtcctaacgg cgcgggaaag tcatccatta
     6901 tccattatct tctggggaca ttcaacggca aggttgtagg agaaaaatcc cagcccaaac
     6961 atttgaccat tagttacgta cgccaaaatt atgaagacaa tcgagggaca ttggcggaat
     7021 tcgcgaagaa aaaccgagta gactatcaag catttttgaa taatctccga aagcttggga
     7081 tggaaagaga tgtttttcat aataaaatcg aacagatgag tatgggacaa cggaaaaaag
     7141 tggaattggc taaatcttta tcccaaccag ctgagctcta tatatgggat gaaccattga
     7201 attatttgga tgtcttcaat caagaacaat tagaacaact gattttgaac gtgaaacctg
     7261 ctatgttact agtggaacat gatcaaacct ttctagataa agtatctact gagattattt
     7321 ctcttgagaa aatctaaacc ttgtaatctc aaagacaaca aggtatggtt aaggagaact
     7381 ttgtcaacga aggagagaat cacatgcttt atgtaacgag acacggagaa acaacttgga
     7441 acgcacaagg attagtttgc gggcgagcag atgtgccgct gactgaaaaa ggacagatgc
     7501 aggcacaggc actagcagaa aaggtagtag atttgcctgt tccaatcacg aaaatcatcc
     7561 attcccctct tcaacgagca agagatacag cacaagcagt agttgatcgt ctcaatttgc
     7621 cgatgaccgc tgatgagcga ttggtagaaa tggattttgg ggattatgac ggactgccaa
     7681 gcaaagatga aaactttcaa aaagcgcgtt tagcgtttgc tgtacgtttc ccaaatggag
     7741 agtcggtctt agatgtgtac gcacggatcg ttcctttatt gaaagaatgc atggaagacg
     7801 aagaaaatgt ctacttgtta gtctgtcata atgccttgat aagagtgatc aatgcttatt
     7861 ttcatccaat gcctaatgaa gggttcttta cattcatggt agacaataca gaattaattt
     7921 cttatgagta attagacttt tgtgttattt aaataaaatg tttcaaggca ctgcttttgc
     7981 ttagcagtgc cttttttgtg gctgtgtcaa ttcttttggc attataaatc caatttttag
     8041 aaataaaatt taattatttt aacaaaagta ttgaaatttt gtttctttag atatatactc
     8101 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8161 tttatgaatg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8221 ggctttgtcg gtattttcac ttccatgcat ataccggaag atgatgtttc ggcttatcaa
     8281 aatcgattga tcgaactagg aagcgaagcg aaaaagaata aactagagtt gatggtcgat
     8341 atttcaggag aggcattgaa acgagctggc ttttcattag aagatctaac acccttgaaa
     8401 gcaattggtg taacagggtt aagaatggat tatcatattt ctaatcagca gatcgccgac
     8461 tggtcacatc aattgaaaat cagtttgaat gccagcacga tcacgcctaa tgatatcgat
     8521 gagttgaaag aagcagcggc tgatttctca cagattgaag cttggcataa ttattatcca
     8581 cgtcttgaaa cagggttgga caaagaatgg tatcaggaaa aaaatcaatg gctcaaaact
     8641 caaggattcc ttgtacaagg ctttgtacca ggagatacag agttgagagg tccactgtat
     8701 caaggattac caactttgga agaacatcga gggatccatc ctctggcagg tgctcttgat
     8761 ctattagcaa gtaacacgga tattgtttat atcggagatg caggtctgtc agaacacgtt
     8821 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8881 gatgaagttt tttacgaata tatattagga aaacatacga atcggcaaga tgatgcaaga
     8941 gatgtcatac gaagcgcaga tgcgcgtttt cgagagattc cgccaatccc agtaagaaat
     9001 acagctaagc gtatgaaagg atctattact ttagataacg aaaagtattt gcgctatatg
     9061 ggtgagatcc aattaactaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagcc
     9121 aaagtcatca aagaagaact accgctgatc aaccaaatca aagctggtat gaactatcaa
     9181 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9241 aacgcagaaa ccaagaaact tttggattag atgaaatgag tatttctcaa gcagtccaac
     9301 tcatgaacaa agaagatcag aaagtggccg ctgctgttga aaaagaactg ccaagtatcg
     9361 agccagtgat cgccaacacg attgaatcct ttaaaaaagg tggtcgcttg atttatatgg
     9421 gagctggaac gagtggtaga cttggtgtat tagatgctgc tgaatgtgta ccgacattcg
     9481 gtgtagaacc ggaaatggtc ataggcctaa ttgctggtgg agaagaagca atgactgtag
     9541 ccgtcgaagg agccgaagat tcttatgaat tagggcaaga agatctaatc aagctgaatt
     9601 taacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgattg
     9661 gtggattaga ttacgcaaaa tcgattggtg ctgttacagg aacgatttct tgcaataaac
     9721 aagcagaaat cagcgagcat gcggatttcc caatcgaagt cgatgcagga ccagaatttt
     9781 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa actgattttg aatatgattt
     9841 ctactatcag tatgatcggt atcggtaaag tattcaataa cttgatggtc gacgtcaaac
     9901 caacaaatga aaaactaatt gaacgctcaa aacgaatcat catgcaagcg acagaagctg
     9961 actacgaaac agctgaaaag tactttgagg aagcagatca aaatgtcaaa ctggctatcg
    10021 tcatgatttt gacaaatagt gataaagagg cagctcaagc aaaattagct gaagcaaacg
    10081 gttttgtaaa aggaacctta tgagggggaa aagaccatgg caaaaaacga attgactttg
    10141 gaagaactgg caaaacaaat ttatcaagga gcaggcggta tgggcaatgt tgaaagcatt
    10201 gtccactgca tgactcgtgt acgtatgaag gtcatcaatg atgatcttgt aaatgtctcg
    10261 gaattaaaag cgattccagg agtacttggc gtagtagagg atgaacagct ccaagtcatc
    10321 atcggacctg ggaaagtgaa caaagtagcg caggaaatgg ttgacttagc aggcgtgaaa
    10381 ttaggagaaa aacttccttc tgctagtgca actacagcaa gttcttctgc atctggaaaa
    10441 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaaccc
    10501 tctaaaatca aatcggtatt aaaagacatc tctaatatct ttgtaccaat gatccctgca
    10561 tttgtcggca ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagct
    10621 ggaacactag acgcaggaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10681 gggatgttta gctatctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10741 acgccaacgc ttggtggagt tatcggggct gttgtgatgc tgacagggat gaatccagaa
    10801 gcaccactga aaaacttgtt taccggtgaa gctcttgcaa ctggacaagg cgggatctta
    10861 ggtgttatct tcgcagtttg gttattgtca atcgttgaga aaaaactgca taaagtagta
    10921 cctgatgcgg tagatatcat cgttacgcca actctttccc tgattgccat cggtctgatc
    10981 gaaattttct tcatcatgcc tttagcaggg ttcatttctg acggcatggt cggtgggatc
    11041 aactgggtac ttggtgttgg aggagcattt gccggcttcg ttctaggtac attattcttg
    11101 ccaatggtca tgttcggact acatcagatc ttgacgccaa tccatgtaca aatgattgat
    11161 gaaactggtc gtaccttatt gcttccaatc cttgcgatgg ccggagcagg acaagtgggt
    11221 gcagcattgg cattatggat ccgctgcaaa aaagacaaag aattaacaga aatgatcaaa
    11281 ggggctttac cagtcggaat cttaggaatc ggcgaaccat tgatttacgg agtaacatta
    11341 cctctaggac gtccatttat tactgcttgt atcggtggag gtatcggtgg ggcagtgatc
    11401 ggaatgatcg gaaatgtcgg agcaatcgct attggacctt caggagccgc tttgattcca
    11461 ttgatctctg atggtaaatg gttaggctat atccttggtt tattggctgc ttacgctggt
    11521 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaacta
    11581 gcgaccgaaa cagccacagt aaatgaagta cctgaaaaag cagtacctca agcttcagct
    11641 gaaagtgtga ctatcttatc atcagttgct gacggaacag ttgtttcttt agataaagca
    11701 agtgacccag tttttgcaca aaaaatgatg ggtgaaggtt actttgtaga accagaaaac
    11761 ggacatatct attccccggt tactggagta ataagttctg ttttcccaac aaaacatgct
    11821 atcggcatta cgacaccaaa tggcttagaa atcttgcttc acatgggcat caacacagtt
    11881 gacttaggcg gtacgccatt cgatgtgaaa gtaacagaag gacagcaagt gacacctgat
    11941 acgcttgtag cagaagctga cttagaagca atcaaagcag cagacaaaga aacctccatg
    12001 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12061 gcgaaagcaa aagcacctgt gatggatgtc aaagcttaat aagaatagaa acaatagaga
    12121 acataaaaag aagaagccga aatttatttt tcagcttctt cttttgtgtt atttattttt
    12181 ttctgccaaa atttgataaa cgcgtgtcac atcagatggt gttccccgta attcataatc
    12241 tgccaagaaa ggaaagccga atctttgtgc gtattgtttg gctgtcagac aatattgatt
    12301 gttgaagttt ttattaccgc taccgactac accaaggcaa agtttatgat tgtcttcgta
    12361 ttccagatat tcgcgcatcg tttcggtcaa gatttctgtg tcaccgttgt ctaacccatt
    12421 cccgccgtct aaataagtag gaacgaaagt aaaaaatggt tctgtttctt tcgtaaaatc
    12481 actattttca tggatttctt ttaaagtaac ttcaggattg acaggatctt ctgcatgcat
    12541 tttttcagca tattctgcta gatgtttggc aaaagcacgc gtatttcctg aaatcgatat
    12601 atataaaatg ttcatggatc atcccccaat tctttccctt tagtatagca tctttttaag
    12661 tttttttggt ggtgtaattt ggaaagggac taggcgcttt gcttgtataa tgagaaaaaa
    12721 gtcggtagaa ttatatttct attaaagttt ttattaatta agtaaaaata aaaaaggttt
    12781 gttatacttt ttgataaaag cggttttact ttggaggatg cttacatgga tacagagcaa
    12841 cagaaaaaag gaattggttt gattccactt gctgccttag tgattggttc agcgattgga
    12901 ggtggcgtat ttggtattat gacggacatc tccggatcgg ctggtggccc agctatcttc
    12961 agctgggtac tcgtaggaac agcaatgttg atgctctcct tatcgatcaa taatatcaat
    13021 aaaaaacgtc cagatttaaa aggcgggatc tttagttacg cagaagaagg ttttggccgt
    13081 ttcgctggtt ttatcagcgg ttggggttac tggctcacat gctggttagg aaacgtggcg
    13141 ttcgcaacat tgttgatgag tgcattaggt tatttcttcc ccgtattcga aggtggtcaa
    13201 aatgtggcat cgatcgtgat gagtacgatc ttcttatggg gatatgcatg gctagtgaat
    13261 cgtggagttg aaaatgctag ctttgtcaat gctatcgtca cattttgtaa attagtgcca
    13321 ttatttttat ttattgtagt agct
//
LOCUS       DACECR010000078        13272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.078, whole genome shotgun sequence.
ACCESSION   DACECR010000078 DACECR010000000
VERSION     DACECR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.078"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(365..2000)
                     /locus_tag="IWO17_002086"
                     /pseudo
     CDS             complement(365..2000)
                     /locus_tag="IWO17_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2323..2802)
                     /gene="rlmH"
                     /locus_tag="IWO17_002087"
     CDS             complement(2323..2802)
                     /gene="rlmH"
                     /locus_tag="IWO17_002087"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ0290947.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            3230..4519
                     /locus_tag="IWO17_002088"
     CDS             3230..4519
                     /locus_tag="IWO17_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0290948.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4558..5163)
                     /locus_tag="IWO17_002089"
     CDS             complement(4558..5163)
                     /locus_tag="IWO17_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ0290949.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5368..5832)
                     /locus_tag="IWO17_002090"
     CDS             complement(5368..5832)
                     /locus_tag="IWO17_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0290950.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5854..6174)
                     /locus_tag="IWO17_002091"
     CDS             complement(5854..6174)
                     /locus_tag="IWO17_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0290951.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6318..7397)
                     /gene="pepA"
                     /locus_tag="IWO17_002092"
     CDS             complement(6318..7397)
                     /gene="pepA"
                     /locus_tag="IWO17_002092"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ0290952.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7597..7947
                     /locus_tag="IWO17_002093"
     CDS             7597..7947
                     /locus_tag="IWO17_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0290953.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            8170..9117
                     /locus_tag="IWO17_002094"
     CDS             8170..9117
                     /locus_tag="IWO17_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0290954.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9174..10592)
                     /gene="pepV"
                     /locus_tag="IWO17_002095"
     CDS             complement(9174..10592)
                     /gene="pepV"
                     /locus_tag="IWO17_002095"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ0290955.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10741..11586
                     /locus_tag="IWO17_002096"
     CDS             10741..11586
                     /locus_tag="IWO17_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ0290956.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11590..12030
                     /locus_tag="IWO17_002097"
     CDS             11590..12030
                     /locus_tag="IWO17_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ0290957.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            12080..12274
                     /locus_tag="IWO17_002098"
     CDS             12080..12274
                     /locus_tag="IWO17_002098"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290958.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12410..13096
                     /locus_tag="IWO17_002099"
     CDS             12410..13096
                     /locus_tag="IWO17_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0290959.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
BASE COUNT         4078 a         2572 c         2433 g         4189 t
ORIGIN      
        1 tttctcagaa acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt
       61 aaaatagcga cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct
      121 ttgtgcttaa attcaaagat aattttctca ataaagattt ttgattccag taagattcct
      181 ttactccttt tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc
      241 cattaaaaat agcccaattc tctataaaga agattaagct tcattattat aatataaagt
      301 ttaaatttgg tttattactt actttttctt attctctagt aagctaatat tgaaaaaaat
      361 gtaatcagat cattattttt atatgttgta acttcctgat aagaaaacta attaaaagaa
      421 agaggtaact acataaacta attaaaaaag ctaacttcat agttggagga taaatggaag
      481 aaacttcaat gattgtttct ccttgtgttc cttcaatcga cactagtcct attatcaatc
      541 tctctttttt gtccatttac ttttgcgact gttccccaat aatcaaatac aggaagcgtt
      601 attttttgtc ctttagattc atgtactgta tagactattt tcgaatccat cacttgataa
      661 ggcatagtat gccattcctt gccaattctt acttcatgat tctgcaaatg ttctttgtat
      721 tgtaatgctt ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt cgtgtaacga
      781 ataagatttt cttgattcat taccaagtga tctctctgag tgacaacttt atgttcatag
      841 tttgcttttg ttgcagcaga tacagccaac agtaataaaa caattgccgt tatattaact
      901 acttttcttt gcttagctga tgtgctcttc atgaaaacga acgataaaat caacgagcca
      961 aacagtactt gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat gggtgtattt
     1021 tgtaataaag accatggaaa aaatttggtg gataagagcc aagagactac cgcactaagg
     1081 taaatcactt tttctttgcg attaaatcct ttaaaaaaga agagtggaca gatcaaagaa
     1141 ataataccaa tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa
     1201 ctatccgaga catttaatgc ttggtgttgt aaggtcgttt tatctggctc attaatcgat
     1261 tgaaccatga tttgtcggaa catcggatac caaaagtatg ctgttaaaag aatcgtcatt
     1321 cctactgcct ttgacaactc cacaatcaac cggatgcttt ctttttttct tgtttcactt
     1381 actacaaagc cgcctaagac tagtaagcca acggctatta cactcataaa agtggaaaga
     1441 acatgtgtat aaatgagtaa gctcatactg actgctaatg gaattgcaca tggctttttc
     1501 tcataaatca gtgcgtaagc ataatacata actatcggta taaccgtaag tgcaatcgct
     1561 tcagcaatag cagaacgatg ataaatatca atcgtacggt acccataaaa tgtatataaa
     1621 cagctaaata taaaagccgc atcgattctt tttaaaaatt ttagtccata atgaaacatc
     1681 agcatgatag tacaaacatt caatagccaa acatataaga tataactaac gattaaatta
     1741 ttacttatcc aataaaaaat gactgccgga aaaaaggtca agtagggata aaaataattc
     1801 actccgctcc cataatgatt aaacgtcgta aaattgattg gaccagaaaa aattgaagat
     1861 aaccctttga ctctagcaaa atggaaggca atatcccccg atgtagaaat agtatgaaca
     1921 tttctaaaat aattgattga ataaagtcca gcaataattg ctaggagaat ggacaatatt
     1981 atttttttct ttacttgcat tacttaatta tatactcctt tcatggattg ctttatctag
     2041 tataacttaa attttaaata aatcgttatt tatttttact ctcacttaaa aaagcactaa
     2101 aattataaga gagggaggta atggtctgaa caccaagaat tgttccaaca tcgaattgct
     2161 taactaatga gttcaaagct taattatgtg gctcttatat ttaccgagcg tggattgcga
     2221 tctctttagc gaaacaaaca tctttcataa aaaaaacagc catatccgct ttcttggata
     2281 tgactattta ctactacttt actatctttc attcttcttg tctcacttat gataaggcgc
     2341 ccccgcattg atccggaagg cacggtagat ttgctcgaca aggatcagac gcatcaattg
     2401 atgtggatac gtcatttttc caaaagagat ctgagcattg cttcttttca tgacggcttc
     2461 gctgagtcct aaagagccgc cgataacaaa aacaaggtgg ctttttcctt ggatacctaa
     2521 ttggtcgatt tgtttagcaa aagattcact tgtaggattt ttcccttcaa ttgctaaggc
     2581 atagacgtat tcttgttctt tgattttcgc taagatccgt tccccttctt tttcttttac
     2641 ttgtctcatt tgcgcttcgc ttaaattctc cggggctttt tcgtcaggaa cttcgatcaa
     2701 ttcgattttt gcataggcat tcagccgttt gacatactca ttgatacctt ggatcaggta
     2761 tttttctttt aattttccta ctgagataat tttgatattc atgttttctc tcctttggtc
     2821 ttcatgctca ctttgtttat tataatcaat tttttcgtct atatgtttat tttggctatt
     2881 tttgttaaaa agatttcccc aacagttatg cacaacatcc acaggtttat ccacagaaaa
     2941 cagtgttatt ttaccttatt tcatcacatt cattaggcaa acatacgttt tataagaatt
     3001 tatccacttt atacacaggc tgtggatatg ttttatccct tatttttcca atcctatccg
     3061 cgtagtttta cacagtatct gtggaaaaag aaaatcaaat attttcatct gaaaacaatg
     3121 atttttccta aagtttttca taaaaagttc acagtctcaa agtagactaa agtcaacaaa
     3181 taagactata ctaacagtag ataaatgaat gtaattggag gaggaacaca tggatagaaa
     3241 aaatgtgaca ccaaagatga aaaaaagtaa aaataatagc ctctggcgta agttaggtct
     3301 ggggcttgtt ggagggatcg ttggaggact actgacagct ggcatttttt atgcagcgat
     3361 gggcactggc aacaatagtt ctactgctac aagcggcaat cagaattctg ccggtgaaac
     3421 agttgtggaa aatgtcaaag tcaatgtaga ttctgatatc accaaagcgg tagataaagt
     3481 tcaagacgct gtcgtttctg ttatcaactt acaaagtcaa aaccaatcaa gcggttttgg
     3541 cggactattc gggcagcaag aagaaagtag cagctcagat gatagtaatt tggaagctta
     3601 tagtgaagga agcggcgtga tctacaaaaa agacgggaat actgcttatg tagtcacgaa
     3661 taatcacgta gtagatggcc agcaaggtct tgaagtcttg atgaaagacg gtacgaaggt
     3721 aaaagctgaa ttagttggaa cagatgctta ttcagattta gcggtgttga agatcaattc
     3781 agacaaagtc gaaacagttg cctcttttgg tgactcaagt gctctgaagg ttggtgagcc
     3841 tgcgattgcg attggttccc cactaggatc tgaatatgca aattctgtca cttcaggaat
     3901 catttcttcc ttgaaccgac aagtgacaag tacgaatgaa tcgaacgaaa cagtcaatat
     3961 caatgcgatc caaacagatg cagcaatcaa ccctggaaat tctggtggtc ctttagttaa
     4021 tatcgaagga caagttatcg gtatcaactc aagtaagatc gcaagtactt ctgaatctac
     4081 ttctaatgta agcgtagaag gaatgggatt tgctattcca agtaacgatg tagtgaatat
     4141 catcaatcaa ctggaaaaag acggaaaagt gacacgtcca gctctaggta tcacaatggt
     4201 cgacttatct gccgtttcta ctcagcaaca agaacaaatc ttgaagattc cttcatctgt
     4261 aactaacggt gtcatcgtga caaatgtcca aacagctaca ccggctgaaa aagctggatt
     4321 gaaacaatac gacgtcatta ctaagatcga tgacaaagaa gtttcttctg gtgtagaact
     4381 tcaatctgct ttgtataaga aaaaagtggg agattctgtc aaaatcactt actatgaagg
     4441 ctcgaaagaa aaaacaacta ctgttgaatt gacgatcgat caatcggcat taaaacaaaa
     4501 tcaaaatagt gaaaattaat aaaaaagagg ttgtgacaaa gacttgtcac aacctcttta
     4561 ttttggaaat gcttctccga ctacggcatc ctcaggcaat actaagattc ctttttttgc
     4621 tggagcattt ggcaaatgca attcacgagc ggaacagatc attccatagc tatctacccc
     4681 tcgcaaagtt cctggccaga tcatcattcc atctggcatc atagctcctg gttttgcaac
     4741 gacaactttt tgtccagctt cgatattggg tgcaccgcaa acgatttgca atactgtacc
     4801 attgtctact tctgtctcag taatcgataa atggtcagaa tcaggatgtg ggacgcatgt
     4861 ttttacgtac ccgacaacga tcttcggttc atgatcagct tcaagctgtt cttcgaatcc
     4921 tgcttctttg atcaatcgat tcaatttcgc taattcctca tctgttaagc ttacttggcc
     4981 gttcccttcg attgttagat gatcggagat atggaaaaag ttccaagcaa caacttgacc
     5041 ttgttcatct ttgatccgag caacattcca tttgcgttca acagtgtttt ctgttccttt
     5101 atcgtcggct ataataacca tcaaggtgtc tccgacgtgt gctttgttat aagcaaaaat
     5161 catgttcttt cctccattat cctttgctga gatttatctt aacaaaaaat tgcattcact
     5221 gcatgaaatt tttttcatgc tttatttccg aagaagcctc ttctggaaca ccatttattc
     5281 gtaacaaggg actgtgacaa gacttttgtc acagtccctt tactgtttta gttaattgat
     5341 taaaaaggtc gtacaagttc cacttgctta attacttggc tgttcttctt cgtcagaaac
     5401 gatcaagaca tcacaaggtg aatgacgtat cacgtagctg gctacacttc ctgtcataaa
     5461 gcgttcgact gcatttagac ctgactgccc aaccatgatt agatcgatct catatttttt
     5521 tggcaatttg actgccatgg attcacgaac agaaccatat gcaataattc cttctatatt
     5581 tgtgtaatca acagatttcc catattcttt acattcttcg atcatttcct tggctgtttc
     5641 agtttgctga tcgactagat tttgatcaat aggtgcatag cccataaagt tataaaattg
     5701 ctgatcgatt acgtttgcta catatacttt cccattatta cggcgagcca cttctaccgc
     5761 ttttttgaaa gcagcaagtg cttgttcact accgtcgata cctactaaaa tattcttata
     5821 ttcttgattc atcttgaccc ctcctaacag atgtcagcct tttgttacac tttctaggaa
     5881 cgattcgatt tcctgttttg ttttacgatc tttattcact agacgaccta attcttttcc
     5941 gtcttggatc acgacaaaac ttgggatacc aaaaatgttc cattcagcgg ctacatctat
     6001 gtacttgtca cggtctactt caataaattg ccaatcagaa aagtcatttt caatttcagg
     6061 catttgcggt ttgataaaac ggcagtctcc gcaccagccg gcagtaaaga aaaatacact
     6121 ctttccagtt tctacgtagc ctgctaattc ttctaaacta tttgggataa tcattctttg
     6181 tcctctactt tctgtttatg tatttttcaa ttaaattata cacctttctt tcgaactaac
     6241 aaacattgat cctcttttta taaaaaaaat ctggcaaaaa gcaatctttt gccagatttt
     6301 atagtttcac tatcgcatta ttttccgtat acgatcgtat ttactgttga tttatccaaa
     6361 cctttcagtg tttgaatcaa catttctcgt gcagcttcaa aatcgcgaat actgaacatc
     6421 gtttgatgcg tatggatata acgtcccact acgccaatca ctgtacttgg gattccttcg
     6481 ttttgtgtat gagcagcacc tgcatctgta ccgccttttg aaacaaagta ttgataaggg
     6541 atattgtttg tttcagcgat atccagcaag tactcgcgca aacgaggaag catgatcaaa
     6601 cctggatctt ggatacgcat caatgtacct tctcctaaat gtccgaatgt gccatttttc
     6661 gtcactgtat catctgctgc agagcagtct actgcaaaga acagatctgg cttgaattta
     6721 ttgacagaag ctttagaacc acgcaagcct acttcttcct ggacattcgc gcctgcaatc
     6781 aatgtatgac ctaatgtttc attttgcaac gcttctaaag cttctaatac cattgtacag
     6841 ccatagcggt tgtcccatga tttactgata atattctttc cgttggctgt tttgatcgtt
     6901 tcagcgtaag gaacgattgt gtctcctgga aggacaccgt aactcattgc ctcttctctt
     6961 gattcaaacc cagcatcgaa caaaatatcg gttacttcta ctgatttttg tccgcttgtt
     7021 cctcgcaata aatgtggggg aacggaagaa gaaatacatg gataattctt accagtagag
     7081 gttttaagtg taaaacgctg agcagaaaca acgtaaggat tccagccgcc tagaggtacg
     7141 actttaaaaa gcccgttttc ttggatttgt gtcagcatga atccgacttc atccatatgt
     7201 gctgcgacca ttacacgggg tgcatccgct tcttcatgat ggcgcagacc aaaaatgccg
     7261 cccaaaccat ctaactgtaa ctcatctacc agcggttcca tatgactttc catataagcg
     7321 cgaacatctt gttcaaaacc gcttgttcct tgtaattctg ttaactcttt gattcgttga
     7381 aatgtttttt cttccatatg ctattcgtct aaatttcaga ctaaactcct ctcagactta
     7441 tattgatctg cggataaaat atgatttttt ctgcagaccg atcgtattta ttatagctta
     7501 ttttttctgg tagcggttat ttatttcaaa gaaaatatga gagtgtggta aaataagcac
     7561 aaggagtaac aagtgagaag aaggaggtat gtccacatgt atgaagaaag cgaactaagc
     7621 tattttaaag gcgggttagc agttggaatc ggtctaggat tattgagcgg ggtagcttca
     7681 acgatttggt atcatcgcaa aaaagtattg tcagcagacc aagtacttga aaatatcaag
     7741 tcatcttttc taaaagaagg tgcaatcgaa ggttcttgga tctcttttga aaaggaacct
     7801 attaggaaat tcgccgttcg gtcgagtgct tatcggggag gtctttctcg aatagaagat
     7861 ggcgaaactg tttattatga atttttagca gatgctcata caggaaccgt attagaaatt
     7921 tcaagaaaaa aaggtgcaac agattaatac gtcaaaaaag tgagagaacg aatacgcacc
     7981 cactattcaa aagagaggta tcatgtcctt cttttgtagt gggtggtttt ttagatagag
     8041 ctttccaaac atatattcca gtattttctt tttaaataaa tgatatttag cgggaaaaac
     8101 tagcatttcc tttgattatg aggtattttt aatttagctt ttgttttcta tagttaagga
     8161 gagtgaaaaa tggaacaaca agaaaaaatt gctgttctga ttccttgtta caatgaggaa
     8221 gcaacaattt caacagtcat tgcagacttt aaacgagaat taccagaagc agatatttat
     8281 gtctatgata ataattcgac cgatcgaaca tacgaattgg ccgttcaagg tggagcaatc
     8341 gtaaaaaaag aacctcgtca aggaaaagga aatgtgatcc gccaaatgtt ctttgatatc
     8401 gatgccgact attatttaat ggtagatggc gatgatactt atcctgcaga agctgtacat
     8461 ggtttgctcg agaaattacg ttctggtgaa gcagatatgg tcattggtga tcgcctatca
     8521 aatggaacct actttgatga gaataagaga cctttccacg attttggaaa taaccttgtt
     8581 agaaatacaa ttactagaat gtataaaaca aaaattcttg atgtcatgac cggctatcga
     8641 ggattcaatc gtatctttgt caaaagtttt cctattatga gttctggttt tcaaatcgaa
     8701 acggaattga cgatccatgc tttagataaa aaattcaagt tcgttgaaat cccaatcgat
     8761 tatcgtgatc gtcctgaagg cagcgaatca aaattgaata cattctctga cggattcaaa
     8821 gtgatcatga tgattattaa aatgtttaaa gattataagc ctttgctttt ctttgggatt
     8881 tggaccattt tcttcttttt atttggattg gtcacgggga ttcctgtgat tcgcgaatat
     8941 atgctgacaa gctttattac acgcatccct tctgcgattt taagtactgg attgatgact
     9001 ttagcacttc tttcattagt aacagggttg attttggata cagtcgtcac gaacgctaag
     9061 aaagaatatg aattgaatct atatcatgtt tatcatgaat accacagtag aaagtaaaaa
     9121 aaggatcaag actgcaaaaa taatttgcaa ttcttgatcc tctttttaat taattatttc
     9181 gccaaacgat agatggcatc tgcatagatt gctgttgctc tgaacaagtc atcaagactc
     9241 ataaattcgt ttgcttgatg cattgtatca gtatatcctg ggaacatcgc tccataagca
     9301 acgccacgtt tcaacaaacg tccgtaagtt ccgccaccga tgatttgttc gtagcctttt
     9361 tctccagtgt ggtcttcata gacttgcaat aatgtagcca ccaatggatc atccattggt
     9421 acgtaatgtg gttccattac gcgagcacct cgagtaactg ttgcgccttc ttgccccact
     9481 gtttgttcga tgtcactttg caatccgtct actgttacac cttttggaaa acggaaattc
     9541 atattgatga agttcgcttc ctctgtacca ttttcttcaa aagcaaatag tccagcattc
     9601 attgtcaatt tgcccatttt ttcatcttca taagcgactc ctagtttttc tccataaaaa
     9661 tcttcatgga cataagcagc tgctacatgg ataaattgtt tagcagatcc taggaattca
     9721 tagttatcta agaacaatgc caagaaagaa cctgcattga taccagattg tgggcttgca
     9781 ccatgtgcgc ctttaccaac taattcgatt tttacatacg tattgtccgc ttcaatcgtt
     9841 ccgcttactg gatttgcttc gatgaattgg taaaatgctt cttccatcgc gatagctgca
     9901 tctgcacttg gtacttgaag tgctgcagtt gctgttcctg gcaccatatt ttcgcgaagt
     9961 ccggaaacaa aagatttcaa gacataatct cctgcattgt cgcctttgaa gcgtaaagct
    10021 aaagaaacat ttcctttttc accattgatg attgggaatt cagcatctgg tgagaaacca
    10081 aaatctggtt cttcttcatg ttcgaaataa taagccatat cgccccagcc gctttcttca
    10141 tcgctgccga ctacgaagcg aacttttttt gatagtttca agtcaagttc tttaatgatc
    10201 ttcatcgcat agtaagctgc catacttgga cctttgtcat cgcttgatcc gcgtgcaaag
    10261 atctttccat ccttgattac tggttcataa ggatctgtgt cccagccatc tcctgcagga
    10321 acgacatcca tgtgtccaaa tatccctagc gtttcgtctc cttcgccaag atcaatatgc
    10381 cctgcatagt tatcgacatt ttttacttta aatccatcac gttcgccata agctaacata
    10441 tgtttcaatg cgtcgcgagg tccaggtcca aatggtgcat ctggtgttac ttttgagtca
    10501 tcgcgctcgc tgttgacacg taaaagattt ttcaagtctt caagcaagtc ttctttacgt
    10561 gcttctactt ctttttgcca atcaattgtc ataaagttaa tcctccactt atttaagtct
    10621 tcgtgttcat gataccacaa attacagaaa aaaatagttc aaaaaatctg acaattcctt
    10681 ttcttatctg tttttctcta aaaaaaatat acgctataat gagtaaaagg aggtttttag
    10741 atgctgataa acgaacaact agtgaaaaga gtcatcacac ctcgtccaaa aatttcacat
    10801 aaaggaacat ttggacgtat cgttttgatt ggcggcaatg aacaatatgg cggagcaatc
    10861 attatgagtg ctttagctgc cgtacacagc ggagcgggtc tcacgactgt agtaactgat
    10921 gaaaaaaatc ggagtgctct tcacagtcat ctgccagaag caatgattgt agtttggacg
    10981 gatactgacc agtttctttc cgtaataaag caagcagatg tactcttgat tggtcccgga
    11041 ttgggtactt cttctaagag tttggaacgt ttcaaacaag tactagaaaa ccaatcaaaa
    11101 gaacaatggc tggtgattga tggctctgct ttaagtttac ttgctgctaa tcatcttttg
    11161 ctaccctatc ccgaacaaac tgtacttaca cctcatcaaa tggagtggca acggttgagt
    11221 tctctaccga tacaagaaca acgggataaa gaaaacaaaa agaagcaaca ggaaatcggt
    11281 gctgtgctcg ttttgaaaag tcatgagaca aaaatttatg gtcaccagca cttctacacc
    11341 aatcctttag gttctgctgc tatggcaacg ggcggcatgg gtgacacttt agctgggatg
    11401 atcacaggat ttttagccca gtttcctcaa aatgaccaaa cgattgcagc agctatttat
    11461 cttcatagtt tcattggaga ccagctggca aaagaacaat atattgtttt gccgacacaa
    11521 atcagcgaag cacttccgaa atggatgaat tatttttgta aaaaatcaat ggaaaacgag
    11581 gcatgagtca tggagaaatt tacaggttat gtgcgagaac ctttttatta tgaaacagat
    11641 caaatgggga ttattcatca cagcaattat atccgctggt ttgaagaggc tcgggtagcc
    11701 ttattggtac atctagggtt tccctataaa aaaatcgaag aatctgggat catcatcccc
    11761 gttctggaag taaattgcag ctataaagaa atgatccatt acggagaaaa agtgagcatc
    11821 catccaacta tccaaaagta tacggggaca cggttggatt ttagctacga aattgtggga
    11881 actgaagaca aaaaattaaa aactactggc agtagtaaac actgtttttt actttctgat
    11941 tcgcaacgat tagtgaagct gagcaaagtc aatccggagt tagatcagtt attccaagcg
    12001 tacgcttctt tggatgatgt aaattcttaa taaagaccgt ctaagcattt ttcttgtatt
    12061 gtgtcgagta gttcgtataa tgaaaagaaa aaagcaggtg atccccatgt atgtcgttaa
    12121 agtaatgcac ggttacatcg ataaaactgg ctgtcgcact cgggaaaaaa acttagacaa
    12181 tctgctcatt ttcaaagaca aaaaagaatc cgaagctttt gctaaaagaa tcggcggtcg
    12241 tgtcaaaccg atccaagagg tacgaccaga ttgattcaat atttttcttt tcgaggctct
    12301 aataggcatg cctatcaggg ctttttttgc agactgtgtt tttatgccca cacgatttgt
    12361 aagcgattta tgttataata gattatatct gcatagaaag gaaggttaaa tggttatcaa
    12421 taatttgcaa cagcaagctt atgaatctat acgtaaacaa atcatttatt ccgagttagc
    12481 acctggaagt aaaatctccg ataaagtgtt agaagaacaa ctagctatcg ggcgtacacc
    12541 gattcgagaa gcactgatcc aactgcgcaa tcaagagctt gtaaaaacca ttccacagtc
    12601 cggcacttat gtttcaaaaa tcgatatccg atctgcttca ttagcccgtt atacgcggga
    12661 aaagttggaa aaaccgatcc tgcaagaatg cagtgcgaaa atgaccaagc aagatcaagc
    12721 taaactagaa aaaatactag aacaaaccga tcaggcagta atcgctcaaa ataaaaaagc
    12781 tttttttgat ttggatactg attttcaccg aacttgttat gaaattgctg gaaaacgaga
    12841 aatttgggat tggctagaaa gttatagtac gcacttgaac cgtttcagat ggttacgttt
    12901 gacgatttca gaattagact ggggtcgagt attagatgaa catcagacaa tgcttcaatc
    12961 aatgattgat cataactttg atgaagtcgg atttctttgc acgatgcatt tacatatgat
    13021 tatcgaggag caagaatacg tgattcataa ttaccccgat tattttgaag atattcagga
    13081 cagacccatc aaataagagg ttttggaatc agcgttttga cctgagtatt agagaaagaa
    13141 acggcaaaat agttcctcat attttgccgt tttttttgag ctaataccga gggtcagctg
    13201 attgcacacc gtttattgag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    13261 gccaagtaaa aa
//
LOCUS       DACECR010000079        13235 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.079, whole genome shotgun sequence.
ACCESSION   DACECR010000079 DACECR010000000
VERSION     DACECR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.079"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            170..1039
                     /locus_tag="IWO17_002100"
     CDS             170..1039
                     /locus_tag="IWO17_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ0290960.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1248..2273
                     /locus_tag="IWO17_002101"
     CDS             1248..2273
                     /locus_tag="IWO17_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0290961.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2312..3262
                     /gene="manA"
                     /locus_tag="IWO17_002102"
     CDS             2312..3262
                     /gene="manA"
                     /locus_tag="IWO17_002102"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ0290962.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3356..5059
                     /locus_tag="IWO17_002103"
     CDS             3356..5059
                     /locus_tag="IWO17_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0290963.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5061..7094
                     /locus_tag="IWO17_002104"
     CDS             5061..7094
                     /locus_tag="IWO17_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0290964.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7105..7545
                     /locus_tag="IWO17_002105"
     CDS             7105..7545
                     /locus_tag="IWO17_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0290965.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7745..8872)
                     /locus_tag="IWO17_002106"
     CDS             complement(7745..8872)
                     /locus_tag="IWO17_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0290966.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8875..9552)
                     /locus_tag="IWO17_002107"
     CDS             complement(8875..9552)
                     /locus_tag="IWO17_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0290967.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9816..11582
                     /locus_tag="IWO17_002108"
     CDS             9816..11582
                     /locus_tag="IWO17_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ0290968.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11597..12034
                     /locus_tag="IWO17_002109"
     CDS             11597..12034
                     /locus_tag="IWO17_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0290969.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12047..13198
                     /locus_tag="IWO17_002110"
     CDS             12047..13198
                     /locus_tag="IWO17_002110"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ0290970.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4494 a         2300 c         2733 g         3708 t
ORIGIN      
        1 gaacagcttt ttcacaacct ctttttataa gagtttttac tcattaattt acaaaaaaca
       61 agcaaaatgc gaattttctc acattttttt taacaaaatc aaagttattg cttgaaagaa
      121 gaagaatcca tcgttaaaat aaagcttgac gtagagggga gcaagaaata tgaaagattt
      181 tcaattgggg gctgccacct atttaaaaag tgtagccatt cgggtgaaag accgagataa
      241 aatgattgat ttttacaaga acgttgttgg ctttgatttg aaaagagaag aaaacgaatt
      301 agccatttta ggaacagaag aagctggaag tgaaatgttg ctgctggaag aaagtcctag
      361 agcgaatgat cacatggggg tgatcaaaaa gctgaaccgt ttttcattga ttatccctag
      421 cacagaagaa atggcagata tcctttgtcg tatccgtaaa atgaattatc caatcaaaga
      481 agctttagaa gatcatggac gtatggggat tttgctcgta gacccagaag agaatgaatt
      541 ggaaatttac catgaagaga ataagaacga ccaaagagaa attccagaaa caatggatca
      601 agaagcatta ttaagaaaat ctgaagaaaa attcagtaaa ctatcaacag gttcttattt
      661 tgaaaaaatc catttaaatg ttactaactt aacaaaagaa cgccaatttc ttaatcatgt
      721 acttggtttg aaggtacata atgaagaaga gggcatccat gtattgaatg aaggagattt
      781 ccatgttggt ttgacagaag ctagcggagg cgcaattgat ttgcctacag atgaagtgat
      841 tggacttgat tttctgaaat tcatgatcga tactgaaagc atggaaggac tgattgctca
      901 cttgcagcaa ctatcagttg atttcttctt agacaaaaag cacacagtac tcacaatcta
      961 tgatgctata ggaatcgaat ggtggttcgt cagtgagcca aaagaaaaga ataaaactga
     1021 cattcgatgc gacgcttaac tttttctttc ttaacgattg tcgcatgtct cgtcatttta
     1081 tgaacggtca tactatcgct tattctagag atagaaagag aaaatagtat accgggggaa
     1141 actgcgcggt ttttcgcgca gtttttttgt gtaaaaaagg ttgattcctc atagattaag
     1201 ctttaagctt ggactgtaag aatctgatag aaaagcagga gaaaaagatg tatccacaca
     1261 cgaaacagct tattttgaaa aagatggaag aaggatttta tccaggtgta gcagtcgcct
     1321 tcataaaaga aaatcaagta gaaacattta cagccggctt tgcccaagtc gttcctgaaa
     1381 agcagctgat gacagaagat ctgttgtttg atgcagcctc actgacgaaa gtcgttgcaa
     1441 ccacttcatt agttttacgt ctattagaag atggaaagat agaactcgat caaccgcttc
     1501 ataaatatct tcctgaattt gaaaatcaga caatcacttt gcgtcattta ctgacacata
     1561 cttctgatat caaaacatgg ataccaaaca gagataagct atctcaggaa gaattgatcg
     1621 ccgcttattt gaagctagaa ccaggtgagc aactaggaaa agaagtaaaa tatacagatg
     1681 ccggtacgat tcttcttggg ttgatgctag aggaaattta ccaagaaact gcagtggaaa
     1741 tctttcaaga acaagtattg gaaccattag gaatgatgaa cagcttattt cttccttttc
     1801 cttccaaaaa aatcgttccc acagaacaac tgccttcggg tgaggttttg cgtggacaaa
     1861 ctcatgatcc gaaagcaaga gttttggctg aacatgcagg taacgcgggt ctctttattg
     1921 atttgaaaga cagcattact tttgtccaaa tgtatttgaa caagggaaaa atccgtaatg
     1981 gtcgtttttt agaagaagag acgattgatt ctttactgca agatcaaaca ccgaaaaaaa
     2041 atggctatcg ttctttagga tgggacttaa aaaaggatgt agaagacggt catgcgattc
     2101 tttttcatac tggttataca ggtacgtttt tcttgattga tataaaaaca caagaagcgt
     2161 tgatattttt gtcaaatcgc gtacatcctg tcgatcatcg tgcggagtat ctcatatgtc
     2221 gagatgaaat tgttgaaaac tttttgaaag agaaagcggt attaccgaaa taatgctata
     2281 atagctatga aaaatgagag gagtttgaat tatgcaggaa cctatgtttt taaaaccagt
     2341 ttttcaagaa aaaatttggg gaggaagtcg tttacgttca gtctttggtt ttgatatccc
     2401 taacgataaa atcggagaag attgggcgat cagtgcgcat cctcatggtg tcagtgtagt
     2461 agaaaatggc gaattcaaag ggaaaagatt agatgaacta tggtcagaac acaaggaatt
     2521 gtttggccat ccttcagaac cagtgtttcc attattgatc aagatcttag acgcagaaga
     2581 tgatctatct gtacaagtac atccagatga tgcctatggg atgaaacatg aaggtgaatt
     2641 aggaaaaact gaatgctggt atatcatcga tgctgaacct ggtgcagaaa tcatctatgg
     2701 tcatcatgca aagacaagag aagaactagc tgagatgatc aaagatgggc gctgggatga
     2761 ccttttgaag aaagtaccag ttaaaaaagg tgatttcttt tatgtaccaa gcggaacgat
     2821 ccatgctatt ggtaaaggaa tcatgatttt agaaacacag caaagttctg acacgactta
     2881 ccgcgtgtat gactacgaca gaaaagatgc gcaaggacaa acaagagagc tgcatatcca
     2941 acaatcgatc gatgtaacaa ctgtccctgc gaaaacacca gaattacaaa tcaaagaagt
     3001 ccgaaaaggg aattcatcga tcgtcactta tttagaaact gagtttttca atgtatatga
     3061 gtgggatatt aaaggaatca catctttcaa aaaacaagcc ccttatacat tgatgacggt
     3121 aatcgaagga gcaggagacc ttgtcgttga cggaaaaaca tatccattag aaaaaggaac
     3181 aagctgtatt attccaaatg gcgtttctga gtgggcggtc caaggtgaac tagcaatcat
     3241 tgcgactgaa ccaggcaaat agttcccaga tagctgaaag cagcagacgg tttttcgttt
     3301 gctgcttttt ctacataaaa tccgtccatc aataaataga agtatggtga aaaagatgga
     3361 atacaaatgg aaagcaaaca acagcagtcg cgtgtctatg aaacaactga gaaaagctgg
     3421 acatcactta aatgaaatga tcatgccaaa tctaagtctg tttatcgctt ggggaatctt
     3481 gtcattagca acaaaaggtg tgtctggcga tttgcacacc acattagaag aggtatgcca
     3541 ttggatgatc cagctgcttt tacccatctt gatcagttat tcaggcggga aatcggtagg
     3601 gggacaaaaa ggggcagtcg ccggtgcaat tgcttcttta gggttgatcg ctcgttccaa
     3661 tgctccacaa atcgtaggag cgatgattat cgggccgctt gccggcatcg tatatgaaca
     3721 atttgtttct agattccacg tgaaattcaa ggctggatat gaaatgcttg tcagcaacct
     3781 actagtagga ctgactggta gcgttatttg tataattgga atcgttttta ttgaaccagt
     3841 tttaaatagt gttcacttgt tgctcgcctc cgttgtgtct tggctagtca caatgaatct
     3901 gttaccactt gtcagtctga tcttagaacc tctaaaagta ctatttttca ataatgcaat
     3961 caatcatggt gtattgacac ctttaggaat cgaattctca caaacagtcg ggtactcttc
     4021 cttatttttg atggaggcaa atcctggacc tggtttaggg atcttacttt ctatcctttg
     4081 ttttgcagaa aaacaggaaa aagtaaatgc gagtggagca ttgatgatcc atgcgattgg
     4141 cgggattcac gaaatctatt ttccatttgt cttgctgcga ccgtatctat ttttagctgt
     4201 aatggctggt ggggcaagcg gcacgttgat atttcaatgg ttagatacgg gattagctgc
     4261 acctgtatcg cctgggtcac tactcgtgat ttttgccaat gcgccttatc cacaactagc
     4321 tggggtaagt atagggatag caatcagtac atttgtgacc ttcatttgtg cagcgatact
     4381 attgaaatta ccaaataaag aaaaagagaa caaaacggtc aaagaaaggg gagaaatctt
     4441 ggaaccatct atcattcatt ccgtcatttt tgcttgcgac tcaggtatgg gatcaagtgc
     4501 tatgggagcc gccctgctga aaaagaaatt agcagcagaa aaaatcgatc tttctgtcgg
     4561 atacagctct atctatgaat tgaataacca agtaaatcag ctggttatcg tgcaacatga
     4621 actagcgggt cttgcaagag aaaaagtacc tcaaagtcaa atctatgtac tggatcactt
     4681 tttggaaaca gatatcttgc tgtcacgaat aaaagaagcg atccaatttc caaagcggga
     4741 agaaaaaaaa gagatagcag atatcacaga acaaaaaaag tcgcctgcta atattgtatt
     4801 tttgtataaa aacaacagac gcggctcaca aactatgggc atgacgattt taagtcaatt
     4861 agcaaaaaaa gcgaaaaaac agtttgttat ctcaaaagaa tcaatagaaa aaatcaaaat
     4921 ccaagaagaa acgatttata tcgcacaaag agaactagct gtaactgaac agctagaaga
     4981 aaagatccct cggctagttc tagttgaaca ttgggtagct acgaatgaat acgaaaaact
     5041 cgtgaaggga gaaatataag atgtatttat cgcccagaga aaaacaatta ttagcagaat
     5101 ttctcagtag cccgtcacct gtatctatcc aaaaaatgat gaatttattg aaggtcagta
     5161 aacggactat ttaccgcgaa ttagataatc ttgaagtctc attgaaatcg gtaaatgctt
     5221 ctttgaaaaa agcagctaga ggctcatttg tcattgaggc atcagaagaa gtattagagc
     5281 aattaaaaga aaatacagat gggaaaagta atgacttatc cactgctaaa cgacaacacg
     5341 cgattttagc cgagttgtta ctgacgacag agccgttgag tcttgctcat ttcatggaat
     5401 catatcgcat cagtaatacc actttttatg cagatatcaa acagttggaa gaaagtattc
     5461 gccaactgcc tttggaaatc attcggaacc agggatacga gatcgaagga ccggaaaaat
     5521 accgccggct tctgacagcg aatgtattag aattagaaat caatgaatat gaactttttc
     5581 atagtatttc tttcgatgca tcactgaatt atttttttcg attcgttgat ccgcaacatc
     5641 tatctcttgc acgcaaagta atcggggaag aattgatcca aaaaaagacc aatctaagtg
     5701 atagaaagct tgaacatttg gttttgatgc tgacattgac gatggagcgt gtgactaaaa
     5761 atcatttact gacaaatgag acatataata gtctggcaaa caaagaatta ctacagacag
     5821 ctaaacgatt gttttcacgg atcgcttctg agacaaaaca actttatcca gtaaatgaga
     5881 tcgtcttttt tgcaggatta ttaaatgact tcacgaactc atttgatcag aatttctttg
     5941 aagagacgtt cgatacacag cttgcttatt tagtaaaaca actgattcaa gaagtaagtg
     6001 aagagacgaa tgtccatttt tatgaagatg aaactttgta caaaatgctg ttgactcatt
     6061 tatctggtgt gttttcaaga gccgttttac aagaagagac actgaccaat ccgattttag
     6121 aaaaaatcat ggttcaatac gaggaagttg cgaatgcaat ccgtaaagca gtagagcgga
     6181 tttttcaaga aaaaaaatta tccgaagaag agatcgctta tatggtcctt cattttgcga
     6241 attcattgga acgaagtccg aaagaaagca atatcagtat tgcaggattt tcgccaagtg
     6301 gattagcttc tactagcatg ttggaactac gattgaaaag gtattttccc ttcatccaac
     6361 aaattcattt ctatcggatt gctgatttaa aaaaaatcga tttacaagaa gaatatgatg
     6421 ttgttatttc tacatctatt ttaccaggat acggtggaaa atatatttta gtctccccgc
     6481 tactgctaga agatgaagtc aagcagctaa aagaagcttt ttcgcatatt gagaagaaaa
     6541 aaaggcaaaa aattacgtat aacagcaata acgagtggat gatagaagat acttatgagg
     6601 aaacaatgca tttcatggat caaatcaatc aattactcgg tcgttttttt atccaagaac
     6661 tggataatcc tgagacgatt tccgaaacga ttcgcagctt atttccttat tttcctgaag
     6721 gactagtaac ggatgaagag gtggttcatc gccggctgct taagcgatat gaacaagcac
     6781 ctatcggtat tcctcataca aatatggggt tattccatac atccagctca ttgatttcta
     6841 aaccgatctt ttgtattttc aatttgaaac atccgttgac cattgaaggg atggatagac
     6901 agccagtcga tttgtcccgt atgttagtta tgctggcgcc ttcgccaatc caagaaaaag
     6961 acgggattct tttaggaaag atcagtggag caatcatcat gaatgacttg aacaccgaga
     7021 tcttccgctc aggaaataaa gaaatcgttt atcagttgtt agcgaaaatc ctgattgaag
     7081 aaataaagaa atgaaggtga aaagatgata cagctaccag cagaaaaaat attgctcgat
     7141 caatcctatc aaaattggaa agaagcgttg accgagatcg gccagtggat gacccaacaa
     7201 gggggaatca aaccggcata tatccaatca atgatcgaaa gacaagaaaa agcgagtgtc
     7261 tatatcggga atttcgtagc cttgccacat gggactgaca aagatgaaga agtagtagaa
     7321 gaaggaatct atttagtcca agtgcctgat ggtgtggact ttggaacaaa agaggaacca
     7381 aaaatcgcga cactcttatt tgtagtggct ttaaaaaaag aacatcagtt agcggtgctc
     7441 caagatttag cctttttctg ttcagacatc gaccaagtaa tggcgctcag tgatgcgaaa
     7501 accattgaag aagtccaaat gatttttgaa catggacata tataaccaat gttcacagta
     7561 aaaaaagctg ccttgcatca agtaataact tcaaccaaga taaaaataag aggagctatt
     7621 tttgttggga ggttcttatt actgcggatg caagcttttg aaaactattt attctagaaa
     7681 accgtttatt tgaaataggg gacggtgaca aggtttttgt caccgcccct attatttttg
     7741 tgttttaata gctctttttt atatagatag tgcccatacc tatgaataat aaaacgatac
     7801 tccatatacc aagtgtcaag ataccattca tgaaagtaac ctgttctgaa gctagtggcg
     7861 agaaaatatc tcctcctatg atcacatttt gaaaatcaac ataagaagtt ggcaagtaat
     7921 gactgattga ttctatccat ccaatcgaag aaaacaagcg atactctgct aagaaaagac
     7981 aaagaagtgt acctaaaaga ttgactagag aatgttcaaa taaagcagat agaagaaaac
     8041 tgattgtact taaaaagagc acccacaaca acaggaacag aaagctctgg aaaagatact
     8101 catacatcgg aaggattcgt atctcctgac caatgatcgt tcctacagga taacggaagt
     8161 ctcccaaacc atttttgatt attactagta tagagacgat caaaaatggc atcacgagac
     8221 ttagcatagc tgcaccataa taaatggtaa aacggatgct atgtttcttt accatactat
     8281 ccggtaatag attgactaga gagattacct gctttctttt ttcataagag atgatggaag
     8341 aaagaaacaa ggcggttata cataaaatca aaaaactaga aatcattcct gaaaaaatat
     8401 tttcgtagta atttgctaaa gggagatgcg ctggcaagtc aaaaatagaa tatctttgga
     8461 tctgatgttc tttcatataa cggagcaact cgacacgttt ttgttgttca atgattggaa
     8521 ttccttgtct ttgaccagaa atcagtcgat ccagtatatc tttttcataa tgataagttg
     8581 cttcgaccat ttgctggaca ttccctgtgt tttttgcatg taagattgct tcgatcagtt
     8641 tgttgctttt ctctaaactt tccacaaatt ctttctcatt ctcatcttct ttcaacgttt
     8701 caatcaaacg ttgcatctct tcatagttat gattcagttc atcgaattct gtataagcaa
     8761 gtttatgatc aaaaaaaagt gggaatgcaa agagcacgac caacaataga ggaaataaaa
     8821 agtttacttt gttttttagt aagagattgc gttcaaatgt aagtaaagac atagttacac
     8881 ctcatataat ttacgataaa tagctaaaat ctcttccata tttgaggatt ctgtgacgat
     8941 ttctccattt tttagaaaac atgctttaga acaaatcgat tggatattcg cgatagcgtg
     9001 ggaagaaaga agaatcgtct tcccctcttt ctgccattgt ctgatgaggc tattcaagat
     9061 atcaatactt cctgggtcta atccgttcaa cggttcatcg aataacatga cagatgaatc
     9121 actgatcaag taagcagcaa gcaagacatg ttgtttcatt cctagagaca tttttccaat
     9181 cggcaaagta aggtactcag tcatcttcaa tcgttggaaa acagtttggc attctacttt
     9241 gctctgccaa tagtatttga tcaaagtcaa ataatcaaca gccgatagat agtcggataa
     9301 ctgattcgtt gattctagaa aaaagaaaga ccggcgataa tctgtataag cagtaggaga
     9361 gatgttgtga agggacaatt gatcataagt tgttggcagc agttgggcaa tgccattcag
     9421 caaagttgtt ttgccaaatc cgttcggggc aatcaaagca gctacttctc ctgattctac
     9481 taaaagattc aattgtcgaa tcacacgttt tttaccacgt ttcatgttca tgtttttgat
     9541 cagtaatgtc atagataggc ctccaatata aaatttaata acataataga atggaaaaca
     9601 tctaaattaa atgctaacaa aagttattgt ttgcatatta taaaaaaatt aaaataacag
     9661 ttttatttaa aattgaagtt ttctgatacc gaaatctttt tattttgctg acatactcaa
     9721 acctttggca caaagtttcg tgccaaagta cgactatttc tctttttagt aagcggatac
     9781 aattagagta tcaaaaggag agagggagag acaaaatgga acggacgatt cccaaaaaca
     9841 acatttcgct aaaagcgagg gtacaaaaat taggcagtac tttatcaagc atggtcatgc
     9901 caaatattgg agcactgatt gcctggggtg tattaacggc tctatttatc ccagatggtt
     9961 atttaccaaa tgaggctttg gctaccatgg taagcccaat gctaacttat ctgattcctt
    10021 tattgatcgg ttatactggc ggaaaagtaa ttgctggtga ccgcggatca gttgttgggg
    10081 caattgcaac gatgggtgtg attgtcggaa cagatattcc aatgatgcta ggcgcgatga
    10141 tcatgggacc tcttggtgga tatgccatca agaaatttga tcagctattc caaaaacgga
    10201 tcaaatccgg ttttgaaatg ctagtcaata atttttcagc aggtcttatc ggttttggat
    10261 tagcgttatt aggtttttct gcgattggtc cagttgtcga tgcattgact caagcaatgg
    10321 caaaaggtgt agagatcatt ttatctgccc atttgattcc attgacaagt atttttattg
    10381 aaccagcaaa aatcttattt ttgaataatg caatcaatca tgggattttg acaccattgg
    10441 gaactgaaca agtagcagag gctggaaaat ctattctatt cttgcttgaa gcaaatcctg
    10501 gtccaggact tggcgtatta cttgctttta ctttgtttgg caaaggagca gcaaaatctt
    10561 cagcaccagg agccatgatc attcactttt taggcgggat ccatgaaatc tattttcctt
    10621 acgttatgat gaaaccgtta ttattcttat cagttatagg cggaggaatg tcaggaagtt
    10681 tcattttcca attgatggat gctggactaa gagcgccagc ttctccaggg tcgatcattg
    10741 cgattctagc gatgacaccg ttcaattctt accttcctgt tattttgggc gtgattgttg
    10801 ccgcagctgt ttcgtttggt atttctgcag taattttgaa agcggatgcg aaagatacaa
    10861 atgaagactt cgaaaaatct gttcaagaga caaaggcagc taagcaagct tccaaaggtg
    10921 cccaaggtat tcctcaagga gctacattct ctggcgtaca aaaaatcatt tttgcttgtg
    10981 atgcaggaat gggatcaagt gcgatgggcg cttcgatctt gagaaaaaaa gtacaggaag
    11041 cagacttgcc tcaaacggtg accaatcaag caatcagcca actgacagat gactcgaata
    11101 cattgatcgt tactcaggtc gaactacaag aaagagcaaa acaaaaagca ccgagcgcgc
    11161 gttttgtctc agtagaaaac tttttgaatt cacctcgata tgatgaaatc attcaagaat
    11221 taagcagcaa gcagaaaaca tcagtttctg aaattgagaa tgagccagca gaagagtttc
    11281 tttcagcaaa catcaaacaa atcaaagaga ttctacttgt ccacgatgat cgttccggat
    11341 cagcaacaat ggggatgacc gtgctcaaag atatattaga caagaaccat ttaactatcc
    11401 cgttaagaaa agtcaatatc caagatttaa aagatgaacc tgattcactg atcgtagcca
    11461 aagaaagttt agcggaaaaa gtacagatgg ctgcacctca atccattcat ttatcagtca
    11521 acagcatggt caatccgcaa aaatacgaat cgattgcaca atatttgaaa cagacagctt
    11581 aagggaggaa aacagcatgc aaagtttgcc attcgacatg atcgagctca atcgatcttt
    11641 taaaaccaaa gaagaagcga tccgctattg cggtcagatg cttgtagaag cagggtgcgt
    11701 tgatgaggaa tatatcgatg ccatggtaga tagagacaaa atgttatctg tttatatggg
    11761 gaattttatc gcgattcctc atggaacaga tgaagcaaaa aaatatgtga aaaaatccgg
    11821 catctgtgtc gttcaagtac cagatggtgt agattttggt accgaagaag aagaaaagat
    11881 tgcgactgtt ttgttcggta ttgcaggagt cggagatgag catttgcaac ttgtacagca
    11941 aatcgcactt tattgcagtg atatggacaa tgttgtccag ctagcagacg cgctcagcaa
    12001 agaagaagta acggaaaatc tagcaattgc ttaaggagag agaaaaatga gagcagtaca
    12061 ttttggtgca ggaaatatcg gtcgaggatt tattggagag gttttggcag ctaatggatt
    12121 tgagattact ttcgtagatg tgaatcacac gatcattgat gctttaaatg aaagaaaaga
    12181 atacgaaatc gaattagcag atgaatcaaa agaaaaaatc actgtaaaaa atgtcaaagg
    12241 aatcaacaat caaacaaatc cagaaaaagt aatccaagaa atcgctgaag cagatttagt
    12301 tactaccgcc attggcccga atatcttacc ttttattgct gagttgattg caaaaggtat
    12361 ccaagaaaga gaaaaggaag gaagcacaac atctttagat atcattgcct gtgaaaatat
    12421 gattggcggt tcagcatttc tagaaaaaga agtgtataaa tatcttccgg aaacatcatt
    12481 tactgacaaa tatatcggct ttccaaatgc agcagtcgat cgaatcgtcc cattacaaca
    12541 ccatgaagat cctttattcg tccaagtgga acctttcaaa gaatgggtca ttgacgatag
    12601 ccaaagaaaa aataaaaaaa tccaattaaa aggggtttta tatgtagatg atctagaacc
    12661 atatatcgaa cgcaaactat ttagtgtcaa tactggtcat gcgactgtcg cttatacagg
    12721 tgccttgtta ggttaccaga ccattgatga agcaatgcaa gatgcgttag ttgtggcaca
    12781 attaaaatca gttttgcagg aaacaggaag tttgctgatt gccaaatggg ggtttgatgc
    12841 agagcagcat catgcgtata ttgaaaaaat cattcatcgc ttccaaaaca aaaatatctc
    12901 tgatgcgatc acacgtgtcg ctcggacacc attacgtaag ttaggttatc aagaaagatt
    12961 tacacgtcca gtgagagaac tacaagaaca taacttgact tgcccacatt tgacggcaac
    13021 aatggggatt atttttaatt actatgatcc tgaagatgaa caaagccgtc aattgcatga
    13081 aatgaagatc catgaaaact tggaacaatt gattcaagaa gtcacaggaa tcaatgatcc
    13141 gaaaacaatc gggaatatca agcaaaacgt gaaccgttat gccaagcaag tcgcttaaaa
    13201 taggatacga aaaaggggaa tgtgacgaaa ttttt
//
LOCUS       DACECR010000080        13130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.080, whole genome shotgun sequence.
ACCESSION   DACECR010000080 DACECR010000000
VERSION     DACECR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.080"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..332)
                     /locus_tag="IWO17_002111"
     CDS             complement(<1..332)
                     /locus_tag="IWO17_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="similar to full-length Gnd, these proteins seems to
                     have a truncated C-terminal 6PGD domainin; in
                     Methylobacillus flagellatus this gene is essential for
                     NAD+-dependent oxidation of 6-phosphogluconate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase
                     (decarboxylating)"
                     /protein_id="HAQ0290971.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQK"
     gene            496..1344
                     /locus_tag="IWO17_002112"
     CDS             496..1344
                     /locus_tag="IWO17_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0290972.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1990..2196
                     /locus_tag="IWO17_002113"
     CDS             1990..2196
                     /locus_tag="IWO17_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ0290973.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(2398..3399)
                     /locus_tag="IWO17_002114"
     CDS             complement(2398..3399)
                     /locus_tag="IWO17_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0290974.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHFDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(3404..5317)
                     /locus_tag="IWO17_002115"
     CDS             complement(3404..5317)
                     /locus_tag="IWO17_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0290975.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(5485..5991)
                     /locus_tag="IWO17_002116"
     CDS             complement(5485..5991)
                     /locus_tag="IWO17_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0290976.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            6151..6591
                     /locus_tag="IWO17_002117"
     CDS             6151..6591
                     /locus_tag="IWO17_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0290977.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(6617..7774)
                     /locus_tag="IWO17_002118"
     CDS             complement(6617..7774)
                     /locus_tag="IWO17_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290978.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(7777..8133)
                     /locus_tag="IWO17_002119"
     CDS             complement(7777..8133)
                     /locus_tag="IWO17_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0290979.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(8431..9351)
                     /gene="sgrA"
                     /locus_tag="IWO17_002120"
     CDS             complement(8431..9351)
                     /gene="sgrA"
                     /locus_tag="IWO17_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ0290980.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSMSSESSTGSTSSESSTGKVN
                     NELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSR
                     TLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9547..10386)
                     /locus_tag="IWO17_002121"
     CDS             complement(9547..10386)
                     /locus_tag="IWO17_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0290981.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10571..11458
                     /locus_tag="IWO17_002122"
     CDS             10571..11458
                     /locus_tag="IWO17_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ0290982.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEGAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(12052..12747)
                     /locus_tag="IWO17_002123"
     CDS             complement(12052..12747)
                     /locus_tag="IWO17_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ0290983.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(12731..13129)
                     /locus_tag="IWO17_002124"
     CDS             complement(12731..13129)
                     /locus_tag="IWO17_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0290984.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3969 a         2569 c         1983 g         4609 t
ORIGIN      
        1 tttttctgaa actcttctgc tcgacggatt gaatctttat aataagagtt tccgccttca
       61 ataataatgt ctcctggtcc gagttttgga aaaagttttc ttaacatgtt ttcggttatc
      121 tctccagcag gaagcattaa ccagatgatt tttttctgct caaaagtagc tagaaattca
      181 gagatctctt gataagcagg aatgtcattt tctcttgcct gactaacagc tgtacttgaa
      241 atgtcgagtc cttgtacttt gtgatggtgc tcttttaagt ttaaagctaa attcaatccc
      301 atttttccca aaccaattac accaatttcc atgatagcgc ctccatttct tataccctca
      361 ttatacaaaa aaattttttt gtaaatagat tttacaaaaa tattttttgt ttgatttttt
      421 cctactctcc ttgtatcttt aacattaatg agaacggaaa aaatgataat cttattatga
      481 aaatgaggaa ttctcatgag cgtctccata agactacaaa tcaaaacaat gtacaacgac
      541 ttcagcccaa aagaacaggc tatcgccgat tatattttag aaaatccaag caaagtaagt
      601 catagtccta ttagcaattt agcaagtgaa ctgggcattg ctgactctac tttttttcaa
      661 tttacaaaaa aacttggtta taatggattt aaagatttta aaatggccat gttgatccaa
      721 gaaaatgatc tatctgctat ttctatccac gaaaatattc agaaaacaga tactgagctc
      781 actatggcgc aaaaggtgtt cgagtctagt atttcaactt taaatgacac aaaaaagttg
      841 ttacaacaaa aggatctaaa aaaagcggct actattatca atgaatctaa acgtgtctat
      901 tttttcggtg ttggcggttc agagattgta gcaactgacg cttatcataa atttttgcgt
      961 tcccctatcg caacttctca cagcacggat taccatattc aattgatgga agcttcttta
     1021 ttgacggaaa aagactgtgc tgttttgatt tctcacacag ggcaatcaaa agaaacaatc
     1081 catattgctg aaaccgtaag aaaaactgga gcaaaaacaa tcgttattac tagccaagct
     1141 aattcttccc ttgctaaact tggcgatgtc gtttttattt ctatttctga agaaacagaa
     1201 ttccgatctg aagctttagc ttcccgtatt tctcaactaa gcattttgga ctctctatat
     1261 gtgattttga tgttttacaa cgaaaaagaa tcacgtcgtt ctatcgctaa agttcgacat
     1321 actatttctg aaatcaaaga atagcaccat aacagaaaaa gcgaaaaatc cataaaacaa
     1381 aaaaagccac taccaaatat ttggtagtgg cttttttggt tctgcaaggg tggtacagaa
     1441 catcaatagt cggtgggtta gaacgtatgt catcgcatct ttggagaagg ttgtattggt
     1501 attattcgag ttacttgtta gatgcgataa caaagttagt atacagtagt aaaaaattta
     1561 tcttttcttt tttttattat ttttgtttct tattttctaa aaaattaaaa aaaattttgg
     1621 tgaattaagt atttccttga taacactggc ttttggcctc attttaggtt atcatttgta
     1681 attttatttc acagttgata tgattgttat gtaaaataaa aaagtttagg atacatcgaa
     1741 tttttgggga aattcttttg ggggtatcct aaatcttttc tattatattc ccatattttc
     1801 tattattggt ctagacgtat gatggtaaac tttgtaccta tctgattgct gaatcattaa
     1861 ttgcttatca ttagccatct ttgtttcaag ttcgctagat gatcatttta ttcacatcct
     1921 aacaaacttg tcacatttat agattcttaa tctatttatc atacaattat aaaaaaggag
     1981 gtgctgctta tgaaagctat ccatatttct ttgctttttt tcgctagtgc attgatttta
     2041 tttgctattc atctttgttc ccagccttat atcaataaca acggaatatt agtggaacca
     2101 gcattctttt gcttaccact cggctttttg tcccttcttg cctccgcagg cttcggattt
     2161 gttgcttttt ttaaacgatc aaaacaacaa atctgactta taaaatttac gtaaaagaga
     2221 tcgtgacaat aatcgtgtga tattcccaaa tttcgaataa aaggtgcccc ttgccatctc
     2281 taactcctca gtcacaagtt acaatattca taaaccatga aaagcttgtt tattgaaatt
     2341 gctccttgtg acttggagct aagtgacaga ttacaacctc ttttgcatca taagacttta
     2401 ctccttgaaa aacattacta acttttgata tgtttcttgc gcttgtttat tgctgaaatc
     2461 aaactgatat tcatggttca aaacagcatt tttctccgca taatctactt ttgtgacttt
     2521 cacccccgct ttctcaaata cagaagttac ttcttccatt tgtttcgtaa acgtatttgt
     2581 atttccatcc gttaagaatg tgttttgcgt ccaacgatcc gcatgtctga ccaaggctaa
     2641 acttttgacc ttctctgatt gttcaaagtc cttattatca aagtaagccc atccacattt
     2701 ttcataaaga aagctagaga ttttatcact tacttgagta agtgcagaag gatccaacag
     2761 accagacaaa gaaataaact tcgtaatctg cttgttctcc aaagtcggat caaaatgatc
     2821 agctttttta atttcttcat tttgaagtgc taacaaatat tctccactaa tttgtgcacc
     2881 tgccgaattt ccaccaatcg aaatattcga ccaatctgct tgatctttta tctctttttt
     2941 agctgcttga atggcttcgt tcaattggat aactggtgta ggatgttttg cttccggtga
     3001 tcgttcataa tctatattta cgatgatttg ctgcgtgtct gaacatagat attccaaata
     3061 atcacgacaa tcatttttat cccctcctac atatgcgcct ccatgcatcc agaaaagaat
     3121 cggtaatttt ttcttttcca ctttcggtgt gtagatatcc atattattat ttttgtactt
     3181 tgaaggatag gatatttctt tttttatatc gatttgttcg attttttgct ggaaatcatc
     3241 tggtcgggca tatacttgtt ctttttcact tgtgtcaaat tgttttctaa caagcaagct
     3301 aatcggcttt ggtgtgattt gattaatcaa aaatacagtg actaaaataa ctacgatgat
     3361 aactggtact gcaatcacta ttcgcttgaa ccatttcatt catttaccct ctctttcttc
     3421 gccaaatcca tgtgataatc gtgctaacag ataaaaatcc acctgaataa atgagaagcg
     3481 aatgtttctt ttctccagtt ttcggcaaca ttcttttttt tgcatctgtc tttttttgta
     3541 ctttcactgt catctgaatc ggttgcgtat catcacttga ttgatcctct cgaggttcat
     3601 tctttccctt attgtctgca ttctctgtcg gatcttgctt ttttggtgga gctactggga
     3661 tttttttgta atagaaaata atagttggta tcgtctcatt aaaaatccct tcagtcggtc
     3721 cttcaacatt tgctaattcg tatccttcga atgtcttttg ctctgtcata taattttctc
     3781 cataagctcc gtaaagctgg atttcgtcct ggtctttgag tagactttta ttgttttcat
     3841 ccacatactt tacgtttatc tgatcatgga acactacttt caaaaagtcc gttacatgtt
     3901 tcgtgtaggc agcatggtca acttcctgtg cctttccatg ccctgctcct tctactaata
     3961 aaagatcttt ataccctcct gataactcat ataatttttg cgcgttagac acaggtacta
     4021 catcatctgc tgttccatgg ataatcaatt taggcaatga atctgacatt ccaatcgtag
     4081 gtagagcagc ttttgtatcc aaatgcatct cctgatcaaa atagtattgt ttcaaaaggc
     4141 ccataaataa atcttcatgc tcttgatccc caatgatgtc taactgattt tttatggcat
     4201 ccggtagtgc gggggctact gtctggctgt acgtaaaacg tagttgttca tcaattgatg
     4261 caaatccgca attttcaata attcctttga cattgacagg aatatctttc gctaatacag
     4321 acatcatcgt agctgctccc atcgaaccac cataaagtaa aatcttttga gatggccgct
     4381 ctcgaacttc ttgatttatc cagtaaagga cgtcatctga ctcatattgt ccataggtca
     4441 cataatcgcc tccgctatta ccagtcgctc tggaatcaac aaataataaa ttatacccgg
     4501 ccttataaaa atcattgtat tcttctgtaa cgatcccgtt attccaattc cctcgaaatc
     4561 cgccatggat catgactgtt ttatctgaac cttgatcgac ataatatgca tatacatcca
     4621 tcattttgcc atcttgtttg tttagtacag tttgatgaag ttgttttgta tttggataga
     4681 agcctgtgtt ttctgtaggc gttgtcccat catctgtacc tccaagcatc gacatcaaca
     4741 cactgttttt tgcaaaagcg atatcgtaca atagataagt agcaatcaga tgtttgataa
     4801 tctctttatc tacaccataa tcattaacag caagctcagt gactaataac tctattaatt
     4861 ttgacttgat gtcttctgtt ggtccattaa gtatttctgt catctgttta ccgaagtaat
     4921 tcaaatattc tttaccattg aaattttcac ctttaccgat atcatcattt tctgtcccat
     4981 tctctttgtc acttacttta gcagaagagg attcgtcgtt tgacattttt gtagctgttg
     5041 cttcttctgt ttgttggtta tctgtggtag cggtcgtttc ttgatctgtg ctagtaacag
     5101 atgtctcaac cgttgtttca gtatttgttt ctgtttgtga agtactcgat ttcgtttcat
     5161 ttccagcatc agtagtttct gtggtttcta cctcccgaga ttccgattcg gaagaaatgc
     5221 tctctccata tactgtccat tgcgggacgc ttgtacttac agcaaataag atgaccaacg
     5281 ctccctttaa tacttgtttt gttctcaagc tattcactcc tttttatgtt tctaggagaa
     5341 ttatatgtaa acgcaaacat tttttataca aaaaataaat ggtttatccc ataaaaatag
     5401 aacaggatta ttttctcatt ttacgtaata aagcagaatg gataaaccag tatccattct
     5461 gcttttttta aacgaagaaa caacttattg ctccaaaaat gtcagaaatc gtttatccca
     5521 aattttctcg taaaaagcga ttgtttcctt tgctttcata aaaggattat cataagttgt
     5581 cgttgctcct ctacacatca taagctggta tcctaatcca tgagcaaact tcacagtcgt
     5641 atctatacaa tactctgctt gtgccccaca taattcgatc gaatgacagt ccaattcagt
     5701 taataaaaca ctcaaattag tcttataaaa tgaattcgca tgggtttttc tcacaaaata
     5761 actgtgctca ggaatagata attccgacaa tagctcccat ggttttgttt gtggcactaa
     5821 ttgagcatct tcgtgttgaa cgaaaataac tggtttccca atcgtttcgt atgttttgat
     5881 tcttttattc actaatgctg tcactgtttc taattcgtgt atcggcgaag aatcaaaaca
     5941 tacccctttt tgtaaatcga tcactactat tgcgtcggct gtgtttgaca tacttgtgcc
     6001 ttctttcttt tttcttgatt taatagtatt tcttatctag ctagttccat tatacgtgac
     6061 aacattttct tattttacac ctctaaatgg cacttttcga ctatttcttc atgttattat
     6121 ttcataaata cttagctatt ggaggaatgt atgatgatta aattgtatac aggttctggc
     6181 tgctcgtctt caagacgcgc aaaacgttgg cttatcgtcc atgatatacc gtttatcgaa
     6241 cagaacttaa ttcgtcaacc actaaccaaa aatgaattta tccacttact aaaattgaca
     6301 aacaacggcg tgaccgaaat gctctctact cattctatta gttataaaaa tcttgttcac
     6361 agaatagatg aactcagttt gagtgaacta tatattgaga ttcgtacatc cccttcttta
     6421 ttgaaaaaac cgattatcta cgaccaatct gcaaaacttt tgactggatt tagcgaagat
     6481 aaaatcagga tgttcctttc taaaactaaa agaaagcaag agctcaacga actcttgctt
     6541 tctggtcatc ttgtcgattt taaagaaaat aatctttttg caatgtctta attttggcat
     6601 aaacaatctg cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct
     6661 cattcctgat ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc
     6721 cattccagta aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat
     6781 cgctaatgac aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag
     6841 tcatagataa tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa
     6901 tatagaatat gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt
     6961 gaaaaatcaa ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta
     7021 ccccactaaa aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc
     7081 tgtcttctgc tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat
     7141 acaaatggat cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa
     7201 tcttgaagtt tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt
     7261 gtactttatc tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca
     7321 taaaataaag gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt
     7381 caaaaaaagg ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa
     7441 atttgttcat ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg
     7501 aaattcttag taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt
     7561 tcgcaaattt ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat
     7621 tttcgtcgaa tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc
     7681 cttctatcgg attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat
     7741 taagttctct taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt
     7801 ttctaaggat tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc
     7861 ctctaaaagt atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat
     7921 catcttgttt tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc
     7981 tgcttgttga atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc
     8041 cttcaccaca tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg
     8101 cctttcattg atatcaataa attcttcaaa caaagtacat actccttttt atctattatt
     8161 tagacttatt ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag
     8221 agatactacc atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt
     8281 tcctgttatt tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac
     8341 aacttcgcca ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt
     8401 attcaatttt agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa
     8461 atagcttgct cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa
     8521 ggttctacta ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc
     8581 ttgatgatca attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct
     8641 ccctgcattt ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt
     8701 tacttttcct gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct
     8761 tcctgtgcta ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgttttctgt
     8821 gctactttca ctgctcgtac tctctgtact gctttcaccg ctcgtgtttt ctgtactgct
     8881 ttcactactt gtgctatccg tactgcttgg cggtccaaca actgtccaac tgcttggttt
     8941 caaattctcc gtgatcgtca tctgtccatc ataaaaaaca atcaaatcaa ctttatcttt
     9001 actcttgatc tcatcaatcg atagttctgc agaaatcatt attgtatact caataccagc
     9061 ggtccattgt acgtctttta aataaacttc cccaataact ttttctgaac ttccattacc
     9121 atcccaacca tatgttagaa cagcatgtac atcactagca ttcacgcttt tgtttggttt
     9181 gactggaata gtaatgactt gttgtgtcat gtcataaaca atccgatcag ttttcacagt
     9241 aaatgaaaga ttctcatttg cccgttcatt tgcagaagta ctgattccaa tcattgctgc
     9301 aattatactc agcccaagaa taacaaataa tacggtcgct gttttcttca tttctatcac
     9361 tctccatata attaccttct atttgataaa ggaattatat aaaatgaaaa tgatgagaac
     9421 acgacagtca gttgtgaaaa tctaacaaaa gatgtcgtgt attcgcttga atttgtttac
     9481 tttttcgttt aatcaatgac agacttaaaa aagcaagacc gatatcagcc ttgctttctt
     9541 catcttttat tgataaacag cagtgtattt cttcatccgc tttgttttat atgcatagag
     9601 taacaaacca acaatcaaga ctaacaagaa taatgtagtt gatatagatg cttcagcccc
     9661 aaagtttccc ccagtaatta ttgtattttg cgattggatc agagtggaga aaaaaccttg
     9721 ttcttgcgtt ccgctgactg gtatacccaa aatagctcct tgtgtccgat tccaagtaat
     9781 atgagcacca atagccgtaa ataaacttcc tgtactgtag ataagtgcgc tgaaaaagca
     9841 acctgctaaa aagatattga aaactgccag aaatgtcatg ttaggattca atccgtgcaa
     9901 aagagcaaat aatgcagcag atccaatcat tgctattaac ggaccaaatc taagcatctg
     9961 atgctgaaaa aagccacgga ataaaagctc ttctgatgcc cccactgcaa taaataaaat
    10021 aaaactcgtt atcagcggca caatgtttgc gtttcctgaa tctaaccgat acgcccccgt
    10081 caaatataat ggactagtaa taagcagaaa cactccaatt ccaacaacga gacctcccgc
    10141 taaatacccc cagtcttttc ctcgaacaga aacaccataa tcttctattc tttttccttc
    10201 gaccttacta gcaagaaata aagctagtaa aaaaacagct aaatgtccga ccgtaccggc
    10261 aattgctcca atccattcac tatttccaat aaacatatcc gcaagtgcat tattgacaag
    10321 aattcctaaa acaaaaccaa ctaagactat taaaacagat ccaatcaacc gaataatcct
    10381 gatcatcttt catccctcct ttatattaag tttaacaacc cttttctttc ttgtctcgtg
    10441 aaaactatgt aaatgttctc catcatagag agaatagatt tttacgaact aagaatatct
    10501 acagcaacct tacgaaaatg aaagactgtc tccctaattc ttctgttaga ctgtttttaa
    10561 taaggaaagg atgggaaatt ttttgaaaaa acaactacta attatgtgcc tttcagcatg
    10621 tgcaatttta ctattctcag gttgtagcaa gtccaatgaa acgattgcaa cgtttaaagg
    10681 tggaacactt acagttcagg atttttatga taacccaaaa gttaaatcaa ataatcaaac
    10741 actattaaaa aaaatgattg tcttcaaagc atttgatgat gtatacggaa aagagatttc
    10801 ttcaaaggaa gtaacaaaag aatataatga acaaatcaaa aaactcggtc caaactacaa
    10861 ggaacaatta aaagcagttg gccaaacaga agagacatat aagaaactct ttaaacagat
    10921 gctggctttc caatacggtt tgaaagccaa tgtaaaatta acagataaag atctagacac
    10981 tgcttggaaa gaattctatc cagaagttag tacacagatc attctatttt cgactaaaga
    11041 aggagcagat aaagcaaaaa aagaagcaaa tgaaggagaa aatttctcca agcttgttca
    11101 agcttatgga aaaaataaaa cattaaagga aactgatgga aaaatgaatt ttgattcaac
    11161 caatccagag attcctactg aagtaaaaaa agcagcattt aaactaaaaa acggggaagt
    11221 ttctgatatc attcctgtaa cagatccaac gacttatcag caaagctatt atctagtaaa
    11281 aatggtaaaa aaacaggata aaggttctaa taaagacaaa tacaaatctg agctagaaaa
    11341 aattgctacc gaagcaaaaa ctcttgatac ggaattcatg gataaaacaa tccgtaaagt
    11401 gatgaaaaaa gacaatgtaa ctattaaaga tccttatgta aaaaatatat tcaaataaac
    11461 aaatttgacc caattgggga tgtggcaaaa tttgagaagt gatttttgga aattccttct
    11521 tatttttgag gcaaaccact tttgccacaa cttcatcggg gccttttttt atttgtacca
    11581 aaacaaatca ctggatttgt tacaatcatg aggatataat ttaaaaaagt tttttatttc
    11641 tatcacatga atcaatgcgc cattaagtag attctttcat gcttccctat tttttcactt
    11701 gccgtatcgc ttcaaaaaca actatcataa actcaaactc gactgaaaag ttattttaaa
    11761 aaagactaaa aatatccctt ctggacttgt tcttcttatt ctgttatact agagaaacac
    11821 gaaagatatt tttatgcgac acttttcatt tgtttttatg aaatttattc aaaactggcg
    11881 taacttcaga gttacaagga tgtattagag gttagggaat acatcgttta aatgagaaat
    11941 aagtaatagt tagtaatagt cacccaaact ttaaattaat gaatattgaa tcgtcagata
    12001 gcctcctcga gttttcgaga aggctatcgt ttattttatg gagaaattga ttcagaagta
    12061 atcagcgacc ttttttagtt caccaatcgt ttctgcttgg aaagtttgca cccaatcttc
    12121 ttggaagccc ctcactgctt tatcaatcga gggcatctct aatacttgat ctagtagctc
    12181 tttacctaac gttatcgcat gagctccaga tcgaatggct gtagtgactt gttcacaatt
    12241 tttgaaacta gctgctaata tttttgttgg tctttgcgta cgttcgatct cagtccgcaa
    12301 cgaatcaatc actttcatag catcaatgcc ttgattttgc atcctgttat aataaggtgc
    12361 aatataatct gctccattat taatagctaa atacccttgt aattctgtgt aaatagctgt
    12421 accggtaatc aaataacctt cttttttcaa ctttttgatc gcttgaaggc ctgcttgatt
    12481 caccggcacc ttgataaacg tatcttttcc cagttcttct atgataactc gagcatcctc
    12541 tatcatttct tcttccgttc ttcctattgt ctgaatatgg ataggaatct gtccgattat
    12601 ttttttgatt tctttcatat gcgagaagaa gtttgacgct ccttcttttt tcagaattgt
    12661 aggatttgtt gttacgcctg ttaaattcaa aaactgttga caagaataaa tactttttgt
    12721 atttgctgta tcaagcatga tttccatata gctttccccc attctgatcc tcttgatcga
    12781 ttttaatgat tgtccccttt cttaaatgcg gcatttcctt atcttctaca tacattgttc
    12841 ctggaagttc cggctcatga gatccatcaa attttatcgt gatatgtcct aattcattca
    12901 aattagtttg gacaacatct cctactgctg tgattacata catttgttta tcaaaacaga
    12961 gcttcatatc acaagttatt tcttttctta cctgattcaa ttcaatctta taacaataat
    13021 ctgttaatgt ttccggtgct tcatctccaa ataaaatcat tattttttct ttcctgaata
    13081 cttctgcttc tataccaaca tctgttacaa ccgtttcaaa gatcgtcatc
//
LOCUS       DACECR010000081        12957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.081, whole genome shotgun sequence.
ACCESSION   DACECR010000081 DACECR010000000
VERSION     DACECR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.081"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..912)
                     /locus_tag="IWO17_002125"
     CDS             complement(<1..912)
                     /locus_tag="IWO17_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ0290985.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1340..1903
                     /gene="efp"
                     /locus_tag="IWO17_002126"
     CDS             1340..1903
                     /gene="efp"
                     /locus_tag="IWO17_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ0290986.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2389..3648
                     /gene="lysA"
                     /locus_tag="IWO17_002127"
     CDS             2389..3648
                     /gene="lysA"
                     /locus_tag="IWO17_002127"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ0290987.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3635..4435
                     /gene="dapF"
                     /locus_tag="IWO17_002128"
     CDS             3635..4435
                     /gene="dapF"
                     /locus_tag="IWO17_002128"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ0290988.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4438..5319
                     /locus_tag="IWO17_002129"
     CDS             4438..5319
                     /locus_tag="IWO17_002129"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ0290989.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5316..6032
                     /locus_tag="IWO17_002130"
     CDS             5316..6032
                     /locus_tag="IWO17_002130"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ0290990.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6038..6733
                     /gene="dapD"
                     /locus_tag="IWO17_002131"
     CDS             6038..6733
                     /gene="dapD"
                     /locus_tag="IWO17_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ0290991.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6749..7750
                     /locus_tag="IWO17_002132"
     CDS             6749..7750
                     /locus_tag="IWO17_002132"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ0290992.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7747..9108
                     /locus_tag="IWO17_002133"
     CDS             7747..9108
                     /locus_tag="IWO17_002133"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ0290993.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9068..10219
                     /locus_tag="IWO17_002134"
     CDS             9068..10219
                     /locus_tag="IWO17_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ0290994.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10194..11363
                     /locus_tag="IWO17_002135"
     CDS             10194..11363
                     /locus_tag="IWO17_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0290995.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11538..12917
                     /locus_tag="IWO17_002136"
     CDS             11538..12917
                     /locus_tag="IWO17_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0290996.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         4316 a         2260 c         2828 g         3553 t
ORIGIN      
        1 gtaaatgttt tttgataaat agcggtctgc tgcgtctgga aaaatcgtaa cgacacgatg
       61 accaggtggt aaacgacgaa tttctcttaa tgctgctgca aaagccgcac cactcgaact
      121 tccaactaat agtccttgtt ctcgtgctaa ctgcctagta tagttaaacc cttctacatc
      181 agagattgtc tcgatctgat tgattgaaag gggagaaaga aacggcggaa tgaattctac
      241 accgataccc tcaatttcgt gaggggctgg atcaccacca tttaacacag agccctctgg
      301 ttctacaccg attattcgaa tgtcgggata tttttctttc aaaaaggtag aagttccagc
      361 aaaggttcca ccactcccaa ttcctgctac aaaactatgg atattttctt tgatttcatg
      421 aaagatttct ggccctaacg tatgatagta agcagcagga ttatctttat tttcaaattg
      481 aagaggcaga tagctgtttg atatctcctc tgcaagtttt ttacttttgc tgatggcacc
      541 gatgattcct tgctcactag gagaatggat gatctttgct cccaatgctt tcatcaattg
      601 ttgtttttct aagctaaact tctctggaac aacaaaaacc gttcttaatt tgtatgtgag
      661 tgcggccaga gcgatcccaa ttcctgtatt tccggcagtt ggttcgatga tcgtcgtttt
      721 ttcattgatt tttccttggg caaatccttc tttgatcaaa taactgccca atctatcttt
      781 gatacttcct ccaggattta aatattctag ttttgcataa atagcagaac ctttaggcat
      841 agaatagtgc tgattgtcaa agcgataaag cggtgtggaa ccaatagctt ctgtaattgt
      901 cgagataatc atgtgggcct cctaaaaaaa gataataaaa aaaggagctg actattaaat
      961 cagctccttg aaagacatag agaaaaattg agtagataaa aagataagtt acctggtact
     1021 cattctatga agggcaatta atagacatga caaatagtat gctgctttta tgcaccatac
     1081 aacagcaacg attcaattgt ttttggttgc tgactgtcat gtgtatccct ccaagtaaaa
     1141 tttttattaa cgatagcatg aaaaaaaata aaagtcaaag gcttgaaaga aaaaaatatt
     1201 tcgatagcat cgtttacccg tgtttatttt tctgacaaat aggcatgaga ccttaaaaga
     1261 tagagaatac tctagttttc tggccggttg tctgatacaa taaagttgat tatgataaag
     1321 aaaagaagga gcaaaaataa tgatctcagc aagtgattta agagccggta tgactttcgt
     1381 acaagacggc aaactgatta aagttctgga tgcaagccac cataaacctg gtaaaggaaa
     1441 tacagttatg cgtatgaaac tgaaagatgt ccgttcaggt gcaacatacg acacaacgtt
     1501 cagaccagaa gaaaaatttg aaaaagcaca tatcgacaca aaacctgtac aatatttgta
     1561 cacgatggat gatacagctt tcttcatgga tttggaaaca tatgagcaat atgaaattcc
     1621 tgtagaaaca gtagaagaag aaatgaaata tatcctagaa aacatggaag taaaaatcca
     1681 atttttcggt agcgaagtca tcggcgttca attaccaaca actgtcgtat tacgtgttgt
     1741 ggaaacacaa ccttccatca aaggtgcaac agtcactggt tcaggtaagc cagcgacaat
     1801 ggaaactggc ttagtagtca atgttcctga cttcatcgaa acagatgaat tgttggaaat
     1861 caatacagca gaaggttctt atgtaaaacg tgcgtcaaaa taattcgcta acggattgat
     1921 aagaatttga aaacagagct tgacctcttg aaaggcagat catcggatct gcctttcaag
     1981 aggttttttg tggaatcagc aaattgaaaa ataaaaaagt atttgatttt atttatattt
     2041 tgaaagatga atggcaaaag ctagagaaag aaaaaactgc acttatttat gaagaaattg
     2101 aaatgattca taaaattgaa aatatccgaa ctattccgaa ctatattgaa gcgaaaatat
     2161 tttttcatca ctaaatagtg ttgaatttat gtttttatgt ataaatagct tctaatagtt
     2221 cgggtatttt gtttttataa ccatttttat tcttattttt aaaaaaatat ttcgaaatat
     2281 actagacaat tcagaaaatt ttgtttaata ttattccaat aaaattcaaa tctttatcaa
     2341 atgaaattcc aataaaaacg gaaaaaaagt gaaaagagga atgaaaaaat gacagaattg
     2401 ataattggcg gagtcacagc aagcacattg gcagaaaagt ataagacacc tctgtatgtg
     2461 tatgatgaag aaaagatgag acaaacgatg ctgtcattca agcgaggctt tgtatcgaaa
     2521 cgttttgata caaaagtgct ttacgcctct aaagcattcc aaacgattga gatgctgcat
     2581 ttagttcgtg aatatggatt cgggctagat gttgtcagtg gtggggaaat ctataccgct
     2641 ttgcaagctg attttcctag agatactatc tacttccatg gtaacaacaa gacgccagag
     2701 gaactgatct atgcacttga aaatgatttg ttgcatattg ttgccgataa tttgatggaa
     2761 gttgaattat tagcagaatt aagccaacgc tatttaaaaa aaatctgtat catgcttcgg
     2821 ttgaatgtcg gagtagaagc acatactcat gaatatatcg tcactgcgca tattgattca
     2881 aaattcggca tgagctatga aagtaaagag tgccaacaag cccttcagtt gataaaagaa
     2941 agcagctatc tagagttaga aggatttcat gctcatatcg ggtcacaaat ttttgatgtt
     3001 actgcttggt tagctgaaat cgacaaattg acgaattact taaccgattt tgaccaacct
     3061 ctttccttga atcttggcgg agggtttggg attcgttata cacaaaatga tcagccgatc
     3121 ccagtcgaag aggcactaaa aaaactgatt gaatatacgg aagaagcttt ggaaaaacga
     3181 aaactgacca ttcgtcaatt actgatcgaa ccgggtagaa gtattgttgg agaagctggt
     3241 acaacactat acacgattgg tttcatcaaa aagacaccac ataaaaaata ttactttgta
     3301 gatggcggga tgacagataa tatccgtcca gctctgtatc aggcagaata cgattgtgat
     3361 ttagcagcaa aactgtcgga agaaaaaaca gagaaagtga cgattgcagg aaaaatgtgt
     3421 gaatcaggcg atgtggtaat taaagaagct tatttgccga aagcagaatc gggtgatctt
     3481 ttagctgttt attccacggg agcttatgga tactcgatga gcagcaacta caatcgagcg
     3541 acacgaccag cagttgtttt tgtcaataat ggccaatcaa gactgattgt tcgcagacaa
     3601 gcattttctg atctactaag gggggaagtt tcctatgatc cttcataaat ataatggctg
     3661 cggaaatgat tttatcttat tagattacgc agaagacaca gattatagtc aattagccgc
     3721 ttctttatgc acaaaagaaa aatatgatat agacggattg atcgccgtaa aaatcgatcc
     3781 gttggaaatg atctattaca acaaagatgg cagccgcgcg ccgatgtgcg gaaatggcat
     3841 tcgctgtttt gctcgatacg taaatgatca gaacatgatg aatgaacagg aattcaacgt
     3901 agagacatta gcaggcacaa gaaaaattac ggtgttggat ccctcacctt tttattgcag
     3961 cgtagatatg ggagtgccag atgattctcc ggaaaaaatc aaagctaatc aaacttatcc
     4021 aataaaaaac caggaaatag agatcaatgg tgaaaaagta acgatcacta gtttattttt
     4081 aggaacgatt cataccgttg tttttgttga aaatgcgcgt gaggaagtct ggagaaaaag
     4141 aggtgaactg atctgtcatc atcctttgtt caaagaaaaa accaatgtca actttgtaga
     4201 agtagtcaat gaacaagaat tgatcgtccg tacctatgaa agaggagtag gttggacact
     4261 tgcttgcggt acaggttgct gtgcctctta tgtggttgct aaggaaactg ggaaaatttc
     4321 agcagatcag gtagttgttc acttggaaca aggagacttg atgatctctg gtgaggatgc
     4381 gatacaaatg gctggcccag cagttcatga atggacaaga atattggagg aatgaagatg
     4441 ataaaaggtt cgatcgtggc gctgatcacg ccgatgaatg aagagggatc agtagattat
     4501 gctggtttgg aaaaattgat ccaattccat ttagatgaac aaacagatgg attattagtt
     4561 ttaggtacaa caggagaatc ttctacgctc acacaatctg aggaggagca gatccttcaa
     4621 ctgacagtaa agaaagtggc tggccgtgtg ccggtgattg caggagctgg aacgaataat
     4681 acgaaagaaa cgattgaaaa agcgaagcat tttgcttctt tgggggcaga cgcacttttg
     4741 gtcatcaccc cttactacaa caaaacaagt gatgcaggac tagctgctca ttttacagca
     4801 attgcagaag cctcaccgat tccgcttatt ttgtataatg ttccttctag aacaggcatg
     4861 tcgattccaa tccatgtatt agtaaattta gcagaacatc ctaatatcat tggtttgaaa
     4921 gaagcctcag gagatatggc ttatgtgatg gatgccgctc ggttgattgg agaagaattc
     4981 ttcctttaca gtggaaatga tgatttgatc ctaccagtaa tgagtgtagg aggaagcggt
     5041 gtaataagtg tatgggcgaa tatccaacct aaaatagtcc atgaattagt aaaagatacg
     5101 caggacggaa gatggcagca agcaaaagaa aagcaattaa atgcacttga actgatccat
     5161 gccttgttca gtgagaccaa tcctattcct gtcaaagcag ccatgtcatt attagactta
     5221 ccttccggac ctctacgcct gcctttagtt tctctttcaa aagagaaaaa gaaacaatta
     5281 gctcagcttt tgttaaaaga aaggacggtt gaaaaatgaa aatcggactg attggcagtg
     5341 gacagatggg aaatagagtc gaagaagttg cacaagaaag aggagatacg gtccttcttt
     5401 tacggacggc acctaaagaa gcaactacgc ttgcttttac ggaactgcca gaactgctta
     5461 ttgatttttc tcatccagat aatttagaga aaatcctttc gtatagctta tcatatcaac
     5521 ttccgttagt cattggcacg acaggttata cacaatcaca gttccaagaa ataaaagaac
     5581 atgccaaaca tgtacctgtg atgtatagtg ctaatttttc gttcggcatc atggtgatga
     5641 atcaactaat caagcaagca gctgcaatgt tgcaaggctg gcagatcgaa ctgattgaaa
     5701 aacatcatag tcgtaaaaaa gatgcaccat ctggtactgc aaatatgctg cttcaaacaa
     5761 tccaagaagt gcaaaagctg cagcccgtat acaactggca agggaaaaaa agagaagaaa
     5821 accaaattgg tgtacacagt attcgtgcag gttctttacc tggtgaacat gatgtattgt
     5881 ttgctgctac tgatgaaata tttacgatca aacatgaatc tttttctaat cgaatctttg
     5941 ctgcaggcgc agtagaagca gcggactggt tgaaagatca gtcaccaggt tattacaaat
     6001 tagaagattt attaatggac aaaggggtgt aaaaaacgtg gaagctcaag aaatcatccg
     6061 ctacattcaa acggcatcta aaaagacgcc tgtgaaagtc tatctgaatg taaccgagcc
     6121 aatcgaattt aaagaaagta aagtttttgg tgaaggaagc agcttcgttg tttttggtga
     6181 ctatgagacg attgctccgg ttttagaagc tgaatcagaa aaaatcattg aaatcgaaat
     6241 cgaaactgct gcaagataca gcgcagttcc tttgctagat attaaaaaaa ttaatgctcg
     6301 tatcgaacca ggtgctatta tcagagatca agtgtcaatc ggcaacaatg ccgtgatcat
     6361 gatgggagcg attatcaata ttggtgcagt aatcggtgaa aacacgatga ttgatatggg
     6421 tgctgtctta ggtggacgag cgacagtggg caaaaactgc catattggtg caggagctgt
     6481 cttagcaggt gtgatcgaac cagcatcggc caaaccggtt atcgtagaag acggcgtact
     6541 ggtaggtgcg aatgcagtga ttgttgaagg tgtccatatc ggaaaagatg cagtcgtggc
     6601 agcaggagca gtggttttag aagatgtggc agcagaaact gttgtcggtg gaatacctgc
     6661 acgtgtatta aaaataagtg atgatcagac aaaagaaaat acagcattga ttgctgcgtt
     6721 acgcgaatta tagaggagat ggagaaaaat gcgcgaaata gatacctgta ttgtgggggc
     6781 aaccggatta gtcggccaaa cgatgctcaa agtgttagaa gaatatgatt ttccagtagg
     6841 acgattgaaa ttattagctt caaaacgttc agccggtaaa gtttgtgtat tcaagaaaaa
     6901 gaaacacact gtacaggaat tgacagaaac ttcatttgaa ggatatgatt tagcgctatt
     6961 ttctgcaggt gcttctgtat ccaaagagtt tgcgccaatc gcacgaagtc agggactatt
     7021 tgtcatagat aattcctcag catggcggga agattcggat attccattaa tcgttccaga
     7081 ggtcaatctg gaagactata cattgaatcg gctgattgca aatccaaatt gttcaaccat
     7141 tcaagcggta cttccgctta aagctttaca agaagccttt ggtatccagc gagtcaatta
     7201 ttcgacctat caagcagttt ctggttccgg gcaaaaaggc attgaagatc ttcatcgtac
     7261 gcaaaatgac gaggaaccag ttttgtatcc ttataatatt agccaaacgg tgattccgga
     7321 aatcgatcag cctttggaaa atgggtatac aaaagaagaa cagaaaatga taaatgagac
     7381 ccgtaagatt ctgcatcagc ctgctttgcc agtatcagca acctgcgtac gtgtgccaat
     7441 cgaaaatgga catggagtct ctgtggcagt tcaactgcaa aaagagttta cgacggaaga
     7501 aattagagag tgtctcagcc agtttccagg gattcaatta gaagatgatc tagcagcaca
     7561 taaatatccg acgagcattc ttgctagagg aacagatatg gtttatgttg ggaggatcag
     7621 aaaagacact tctttagaga acggattgtt gttctatacg actgccgata acatacgcaa
     7681 aggagccgct gcgaatgccg tacagattgc tgcagcttta atgcagaaaa tagcggaggt
     7741 ggcaagatga gtaatacgct gaaagtagcg aaattcggcg gaacatcgat gtctcatgaa
     7801 gagcaattca aaaaagtccg tacaattgtt ctttcagatc ccaatcgcca agtgatcgtt
     7861 gtttctgctt taggcaaacg tacgtcagat gatgacaaag taacagactt actttatttg
     7921 atttatgctt accttcagca tcatgttgat tggacacccc tttggaaaag gatttgtgat
     7981 cgtttcattc aagtaagaga tgttcttgcc ctgcagttac ctattgaaga tgaactagca
     8041 cgcatacaga aacaactgga agacgaaaag ataactgaag attatttagt aagtagaggt
     8101 gaatatctga ctgctcaact gatggctgag tatcttggtt accagtttat tgacgcaaaa
     8161 gacctgattt tctttgacta tgatgggaaa atcgatcagg aaaaaacagc acaggaaatc
     8221 acaaaagcat ttaaaagata tccgaaaatg gttgttccag ggttttacgg ggcgaaccct
     8281 gcaggaaaag aaaaattatt gggccgtggg ggaagcgaca ttaccggcgc gatactggcg
     8341 aagatgttgc aagcggaaag ttatgagaat tggacggatg tttcaggaat catgatggct
     8401 gatccgcgaa tcatcgatca tcctaaaatc atcgaggaaa tctcttttag agaactgaga
     8461 gagatggctt atatgggggc taacgttctt catgaagcag ctgttttccc agttcaagag
     8521 gctgatatcc ccatccaaat aaaaaatacc aatgatcctt tcgctcaagg gacaaaaata
     8581 tcgaatcacg aaatagaaaa ggaaaatgga ctgacaggaa tagctggacg caaggatttt
     8641 ctttccatca ctttattcaa acgtcatatg tccgatgaaa tcgggtttat ctggaaagca
     8701 atgagcattt ttgctaaaca tgagatatca atcgaacata ttccttcagg gatcgacaat
     8761 atcggagtga tcgtctcagc agaagcgatt gccgataagt tatttctcat cacgaaagaa
     8821 ttaaaagaaa cacttggcgt ggaagaaatc gtcgtgatcg aagacttagc tttgatctct
     8881 gtcgttgggg gtccgcataa agaattgatt ggattatctg ggaaagtgtt atcgatattg
     8941 aacaaattag aaattcgcac atcgatctta tcacaaggag cacaggagtt gaatctgatc
     9001 atcggtgtac cgaataatca atatgaaaca gtagtgaagg gtatctatga agggatggtg
     9061 aatacggatg ctgaaagaat atcgaatggc actccatcaa attcctgaaa tcgggtttca
     9121 tgaattcaag acaaaaaaat atttatatga acaagtaaaa aacagcgagg ccgttatcca
     9181 tgaaatcggc gaaacaggac tactccttta ttttgataat cagcaagaag aaacgattgc
     9241 ttttcgaaca gatatcgatg cactgccagt caaagaagaa acgaatctct cttttgcttc
     9301 taagcatgaa ggttatatgc atgcatgtgg tcatgacgga catatggcga tgcttttagg
     9361 gttgactgac tttataacta aacacattga ggatcttatt tataatgtag tactgatcta
     9421 tcagccatca gaagaaattt ccggtggcgc acaaagcgtt attcagtcag gattgttgga
     9481 taaatatgaa gtacaagcaa tatttggctt tcatctatgg cctggactag ctaagggaga
     9541 agttttctct cgaccaggac ctttgatggc acaaagcagt gaaacagaca ttatcattaa
     9601 aggccagtct gcacatattg cttctagtga aaaaggaatc gatagtctgg aggcagctgt
     9661 acgatttttg aagaatgttt atgattttga ccggcaatta ccggcagagt ggaatcatct
     9721 aatgaaattt ggccaaatcc aaggtggaac gatccgaaat gttttagctg gagaagttat
     9781 tgtatccgga agtatccgtt cttacagttc agatatgcaa aattatttga agaggggatt
     9841 agaccattta gcagaacagt ttcactctac agctactatt tcttttcgct ataatgacgg
     9901 ctatccagct gtaatcaacc atagaggctt atatgcagca ctgttagatt caaacaaact
     9961 acatgaatta gatgctccag ttcttcaagc cgaagatttt ggcatatata cgcaacaata
    10021 tccatgtgta tttttctttt taggtgtcgg tgatacccct gctttacatg atccgacgtt
    10081 cgattttgat atggatgttt tagaaaatgg tgtagaatgg tacaaaacga ttctttttac
    10141 agaaaatctt cttgtaaaaa tcgatagcag ttcaaaagag gagcgagtaa ataatgatga
    10201 atttatccaa tcgatttagt cctaggcttt ctagaataga agtatcaaaa atacgacaat
    10261 tcgatcagca aatatcgtcg ataccagatg tgatcaaact caccctggga gaaccggatt
    10321 ttccaactcc tgaacatgtc aagcaagcag gaatagcagc gattgaagaa gacttttctc
    10381 actataccgg aatgagagga ttagaggaac tgcgggaagc ggcttgtata tttcagcaac
    10441 agcgttatgg attaacttat gatccgcaaa cggaagtttt gacgacagtt ggtgcaacgg
    10501 aagcgattgc ctctgcgttg ttatctgttt tggaagaagg agacaaggtt cttattccag
    10561 cgcctgctta ttcaggatat cagccattag tggaactagc tggtgctgaa ttgatcccga
    10621 tagatacttc tgacacaggg tttgtctgtc agccggaaca atttgaacga gcatttgaac
    10681 aatatggatc agctgtcaaa gcagtcattt taaactatcc taacaatcct actgggacga
    10741 cattgtctgc aaaacaattg aaagcaatcg ctgaggtttt aaaaaaatat ccggtatttg
    10801 ttataagtga tgaagtatat gctgaattga cttatagtgg aacgcatatg tccatagcta
    10861 cttatcttcc agaacaaaca atcgtgataa gcgggttatc caagtcacat gcgatgactg
    10921 gttggcgagt gggctttatt tttgctcaaa aagcccttat tgacgaacta attaaagtcc
    10981 atcaatatct ggtgactgcc gctacaacga tgtcgcaaaa agctgcggct gaagcgctaa
    11041 taaatggggt gaaagatagc gagaatatga aagagcaata tcaaacacga agagactatc
    11101 tggtccagca attggcgcct ttaggatttg aagtgaccca gccaaacggt gcattttact
    11161 tgttttgcaa actcccagct gccattcaaa ctgactcttg gcagttttgt gtagaattag
    11221 cggaacaggc aaaagttgcg tgtatcccag gaattgcatt tggcccagaa ggagaaggtt
    11281 atatccggat ttcttatgct agcagcatgg aaaaattaca tgaggcctgc aaacggatca
    11341 aagacttttt aacagaacag tgattcatag attttatcaa aaaaacaatc gttaattttg
    11401 cggtatattc tgcaaggaga cgattgtttt ttaaatttag ttcaaatgaa cgcgtttctt
    11461 ttgattcata actaatgtaa tgttaagttt aaattgttta gaatgatttt aaaagaatat
    11521 tcaaggaggt ttcatcaatg gaaaaaatca aagttgtcat tgtaggtgca tctcatggag
    11581 ggcatcaatc gatcttggaa ctattgacac gatacggtga aaatgtagat atcacgctat
    11641 ttgaagcagg agactatatt tccttcatgt catgtggtat ggagctgtat ttagaagaca
    11701 aggtaacaga tgtaaatgat gtacgtaatt tcagaccaga aaactttccg caatccaatg
    11761 ttcatatttt aaataatcac gaagtaaaaa cgatcgatgc cgataaaaaa acagtaacgg
    11821 ttactagcgc atcagataac gaaacaaaag aatatcctta tgacaaattg attttgagtt
    11881 ctggtgtcaa accaaacaat ctgccagtac caggagcaga tctagaaaat gtctacttga
    11941 tgcgtggata caattgggca accaaaatca aagaaaaatt aaatgatccg gctattaaaa
    12001 aaatcgctgt cgtaggtgcg ggttacattg ggatcgaagc agcagaagca agccgaaaag
    12061 ctgggaaaga agtcgtattg cttgatgtga tcgatagacc tttaggcact tatttagatg
    12121 ctgagatgac agatatttta gaacaacacc taaatgaaaa cggtattgaa gttttgacaa
    12181 atgcaaaaat cgaagctttt actggtaacg gaaaagttga ggcaatcaaa acaagtgaaa
    12241 aagaaatccc agcagatcta gtcattcaag ctgccggtgt gaaggcaaat acagaatggt
    12301 taaaagggat cgtcgactta gatgaacgtg gctggatcca aacaaatgaa tacttgcaaa
    12361 cgaatcttcc agatgtgtat gcagtaggag atgctacact tgcttattct atcccagcac
    12421 gcaaaaaaat gccgatcgct ttagcgactg ttgcacgtag agaagcacgt tatgtagtaa
    12481 aacatctttt tgaagaagta ccaaacaaac cgttcggcgg agtcgtaggt tcctctgcat
    12541 taagtgtctt tgactatcat tttgcagcaa gcggattgaa tagctttaca gcagcaaaag
    12601 caggagtaga tgtacgagtg tcttattatg aagatacgtt acgaccaaaa tatgtaccag
    12661 aagaacaagg aaatccaaaa gtgtccatcc aattattctc ggatccaatg acccatcaat
    12721 tgctaggagg agcagtatta tctacctatg atgttacagg tcaaggaaat gtattggcac
    12781 tagcgatcca aaacaaactg actttagaag aactagcaga agctgacttc ttcttccagc
    12841 caggctttga ccgacaatgg agcatgttga atctagccgc tcaacaagcg ttaggagaag
    12901 aaccatttgt agaataagaa aacgaaatat ttgaaaagag gttgtgaaaa agctgcc
//
LOCUS       DACECR010000082        12891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.082, whole genome shotgun sequence.
ACCESSION   DACECR010000082 DACECR010000000
VERSION     DACECR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.082"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(260..979)
                     /locus_tag="IWO17_002137"
     CDS             complement(260..979)
                     /locus_tag="IWO17_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0290997.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1028..2674)
                     /locus_tag="IWO17_002138"
     CDS             complement(1028..2674)
                     /locus_tag="IWO17_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0290998.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2853..3443
                     /locus_tag="IWO17_002139"
     CDS             2853..3443
                     /locus_tag="IWO17_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0290999.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3535..5040
                     /locus_tag="IWO17_002140"
     CDS             3535..5040
                     /locus_tag="IWO17_002140"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ0291000.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5124..6476)
                     /gene="celB"
                     /locus_tag="IWO17_002141"
     CDS             complement(5124..6476)
                     /gene="celB"
                     /locus_tag="IWO17_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0291001.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6476..6994)
                     /locus_tag="IWO17_002142"
     CDS             complement(6476..6994)
                     /locus_tag="IWO17_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291002.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7010..7336)
                     /locus_tag="IWO17_002143"
     CDS             complement(7010..7336)
                     /locus_tag="IWO17_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ0291003.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7349..9328)
                     /locus_tag="IWO17_002144"
     CDS             complement(7349..9328)
                     /locus_tag="IWO17_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0291004.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9349..9663)
                     /locus_tag="IWO17_002145"
     CDS             complement(9349..9663)
                     /locus_tag="IWO17_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ0291005.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9831..10124
                     /locus_tag="IWO17_002146"
     CDS             9831..10124
                     /locus_tag="IWO17_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ0291006.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10202..11314)
                     /locus_tag="IWO17_002147"
     CDS             complement(10202..11314)
                     /locus_tag="IWO17_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0291007.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11701..11880
                     /locus_tag="IWO17_002148"
     CDS             11701..11880
                     /locus_tag="IWO17_002148"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291008.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12074..12244
                     /locus_tag="IWO17_002149"
     CDS             12074..12244
                     /locus_tag="IWO17_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0291009.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12668..>12891)
                     /locus_tag="IWO17_002150"
     CDS             complement(12668..>12891)
                     /locus_tag="IWO17_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0291010.1"
                     /translation="TFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTG
                     ETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4075 a         2586 c         2187 g         4043 t
ORIGIN      
        1 aggttgtgaa ttcagcgttt tggcctgagt attagggaga tcgtgtcctt actatctaac
       61 tctgagtcac aaggaacaat ggggactgta acaaaagtct tgtcacagtc ccttattccg
      121 aacaaacggt gttccagaag tagcttctac ggaaataagc cgaaatttca caaaaaagcc
      181 atccgaaagt cagacggaca agtctgcttt tcagatggcg ctacaactac tctgcttatt
      241 cttttagcta tccattcaat tatttttctc tcacttgttc tagctcttct ttggtcatgg
      301 gtcgatattg ccctttattc aaatctttag gcagccaaaa ttttcccata cggattcttt
      361 ttaaatataa gacttctttt cctactgctt tgaccattcg cttaacttgg tgaaactttc
      421 cttcatgcaa aatcagacgg ataattgaag tttctgtctc ttgatctact tgatcaattt
      481 ggagttttgc cggcaatgtc tgctcatcgc catcaataat cagtccttct gcaaataaac
      541 ggatatcttc ttctgtcatt ataccgctaa cctcagctag atattctttc tccacatgtt
      601 ttttaggtga tagtaactga tgggcaaaca ccccgtcatt cgttaaaatc aagaggccct
      661 ctgtgtcttt atctaatcgg ccgactggaa aaagatcttc ccgataatcc tggtcttcca
      721 ataaatcgat tacggtttga tcatgattat cctgagtagc agaaatgact cctgcaggtt
      781 tatgcaaaat gtagtaaaaa tctttttgat acgtaatgat ttctcctaaa taagtaaccg
      841 catcttcgaa ttcatttact tgcgctttat cgcttttcac cacttgtcca ttgacttcga
      901 tcgttccttt tttgattaat cctttgactt ctttgcggct gccaagtccc atatctgcta
      961 aaaacttatc taaacgcatg cgtttccccc atttcaaaag agcatcaatc tagctcttct
     1021 gattcactta ttttattttt aatttatgtc gtaaacgcgc tattcctggt cctagtagtc
     1081 tttctgccaa gcggattttc aaagctaaat aaccatacac taaagcccct atacctgcta
     1141 ctacaagaat caacaataga gactggaatt tgctatcttc gtttaaaaaa gcaccaaaaa
     1201 tcatctttgt gatccacgaa gccaaaagca taatcaaggt aagaataaag atcagcaatg
     1261 tcctgcgcca gacaaaactt ggtttaaagc gggctacttc atggattctc tttaagatca
     1321 aggtacaaga cacagcaaat ccgatcattg tcgccaataa agggccgtat acttcaaata
     1381 aacgaatggc tgggtattga agaaggagtt tcactagaaa cccaataacc aaataattaa
     1441 ttgccgcctt gttttcaaac attccttgca gcatgttgga aactagcata tatagtccta
     1501 ggaagagtcc tacaaaactt gcttggatca gtacattact tcctagagaa tccggtgtat
     1561 aaaataatgt atttagcgga taggcaagta acataacacc aaatgtcgcc ggaaacataa
     1621 aaaatgaaaa caactgtaaa tttccgcttg tcaattttgc cagacctact cgatctttca
     1681 acgtaacagc ttctgtaatc aacggtaacc cagtagacgc gatcgatgtt gccaatgcaa
     1741 taactaccat cgtcaactta tctggattgg cactaaaaat cgaaaaaaga tccagtaact
     1801 gtgcattgga atactcggtg gatgcagaca tgatcttcat aaaagtgaac tgatcgacta
     1861 atttgaagat cgttacccct gaacccacaa taataaacgg aatcgcttct ttaacggtat
     1921 ctaacaacaa ttccttcgtt gcaatattta ttttgttttc gctgtagtga ataaacgccg
     1981 atgtatacgc ttgatgtttc ttcaaaaaat acaaaaggac aagaatactt gctagcatac
     2041 cgataaatgc cgcgaaagtt gattgcgtga ccgccgtcac atagtcacca tccatgactt
     2101 tcatgatgat gaatgtagcc aatagcatat aaaaaatacg tgccacctgt tctacaatct
     2161 gcgacagcgc ataaggcatc atttcttgat tgccttgcaa ataccctcgt atcacactca
     2221 tacaaggaaa aaccaataca gcgatactca aagaacgcat cgttggaatc agttcttctc
     2281 cgccaccaga agcatgagct agccaaggag aggcgatgta catgaacaaa gcaaacaaca
     2341 ggcctaaccc gcccatcatc tgtaatgcac ggataaataa tcgtcgtgaa gtgccatatt
     2401 cattcaatga attatagcgt gcagtttgtt ttgcaatcgc agcaggtatt cctgctgtag
     2461 agatcagtag aaacaatgca tatatattgt atcccatatt gaataatcca ttggcagcct
     2521 tagcattttc gcccatccaa gcataccagg gaatgatata gacagcgcct agcaatcttg
     2581 aaatgatatt actggcggtc atccaagcag agcctcgtgc cattttttct tgcggtgtta
     2641 attgatctaa tgctggtttt ctttgattat ccatcggtca gattccaact ttctacaaaa
     2701 cattactatc attttaattg gtaaaacaga aactggcaat cgattttata taacttttat
     2761 aagaacaatt gtttttttga attcggtttc gtttttttgg taaagtaaag aaatatacag
     2821 ccttttatgg ctaacattta taggagatat caatgacaca agaaataaaa aaactacccg
     2881 ttatattttt aggtcttttc tttatcacag ttggattgaa ttggttcttg ctccctcacg
     2941 atatagcttc cgccggagtg ggcgccatcg gacatttagt tgaaacggcc ttttctatcc
     3001 aacgctcagt tactgtttgg agcatcaatc ttatgatgct gcttctagcc gctgtcctat
     3061 taggaaaatc agtatttttt aagactgtag cgggaagtct gctttttcct gttgttttag
     3121 agattgttcc aaaagtggaa atgatctcct cacatttctt ttcactgatt tcaggaagtc
     3181 ttttgttcag tcttggggtt tacacattgt acacagtcgg ggcctcaaat ggcggtataa
     3241 cgataccgcc gattattttc cacaaatttt ttcggcttcc aatgccgcaa ggacttttac
     3301 ttaccaatag tcttatcgtc tttttgaatt attatgtttt tggattgttg gagacactat
     3361 tcgttctcct atctattaca gtcagttcat tgtttatgaa aatactcatc cgcttgtccc
     3421 ctgtgtcaag aaaatttgaa taaagctgga ttttgtcata gcggaaacaa acgatatatt
     3481 aaaaagagtg tgctataata aaccaagaaa ttgaataaag cgagggatca atcaatgagc
     3541 ttctctttag aagacatccg tcatttacta ttaaaagaaa atctattaaa agaatttgtt
     3601 tcaacaagag gctggcatct tgatgtacca gaaaaagctg aatttcttca gttaagttat
     3661 gattcaagaa aagctgatgc ttctactttg tttttctgta aaggaatgaa tttcaaagaa
     3721 gaatatttgg attcagcaat cgaacagggt attcaatatt atgtatcaga acaaccatac
     3781 gaaaattcag cagtaggaat catcgtaacg gatatccgta aagcaatggc gcttcttgct
     3841 atggcttttt acgactatcc gcaaaataaa ttgaaagtga tcggattcac tggaacaaaa
     3901 ggtaaaacaa ctgctgcgta ctttacaaaa gcaatcttgg atcacacaac aaataaaaaa
     3961 acagcattgc tttctactat gaatacaacg ttagatggca aaacatattt caaatcccat
     4021 ttgacaacgc ctgagtcatt agatttatat cgtatgatgt cagaagcagt tgaaaatggt
     4081 atgactcatc tagtgatgga agtttcttca caagcttaca aaacacaacg cgtatacggc
     4141 ttgactttag atgtcggaat cttcttgaat atctcgcctg accatatcag tccgatcgaa
     4201 catccgacat ttgatgacta tttctattgc aagcgacaat tgatcttgaa ttcaaaaaca
     4261 gttgttttaa atcatgaaag tgactatttt gatttactta gggaaacagc cgatctattc
     4321 cagattccaa cgatcactta tggtcgttcg gaagaaagca attatcaagt gatccgttca
     4381 gataaaggta ctcacggttt tactttatct tctcatgaag atcgcttagc tatctgcgat
     4441 actgcttatg atattttgct ggctggagga ttcaaccaag aaaatgcagc cagtgcgatc
     4501 attgccgcag cactaacagg tgcctccagt caagatgcac aagacggatt aaaagaagct
     4561 cgtgtaccag gccgaatgga tcaattgatc caatccaatg gagcgcatgt ctatgtggac
     4621 tatgcacata attatctaag cttgaaaaca ttgctgaaat ttgccaaaaa tgaacatcca
     4681 gatggccgtg taattgttgt attaggcagc ccaggaaaca aagcaatctc ccgtcgacat
     4741 gattttggaa aagtgctatc agaaacagct gacatcgcct ttttgacggc agatgaccca
     4801 gcatttgaag atccgaaaaa aatcgcggaa gaaatcaatg aagcaattac taatccagat
     4861 ttgatcgttc attatgaaat ggatcgtcct gaagcgattc gacgcgcttt agcagaaagc
     4921 gctccgcaag attcagtcgt tattgcggga aaaggcgtgg atccctatca aaaaatcaat
     4981 ggtgtagatg aaccttatga aggggattat gcaatcgtga agcggttgat tgaagaataa
     5041 tggtatttat atactagtgg tttcccattt ctaagaaaaa aagggactgt gattttttgt
     5101 cacagttcct tttttcttag aaatcatttt aacttttcac tagctaattc tgctgccttt
     5161 tctctttctt catttaataa tttattatca tagaatttga taaatgggaa ccagataatg
     5221 aatgcgatga ttgcacaaat gactgctaac acagcagctt tccaatcacc accactacca
     5281 acaaatgctc cgatcccgac tggtgaaggc cacggcatct gtgcaagcat cggtttgacg
     5341 atttccagcc gaatggcaaa ataagcgagt gtagcagaag ccatcggagc taagaaaaac
     5401 ggaaccgcta aatagggatt gtagacgatt ggcatcccga aaatgattgg ttcattgata
     5461 ttgaataatc ctggtacaag tgaggctttt cctaacactt tcagctgatc agatttcgcg
     5521 aggaatgcaa taaagatgat caatcctaaa gtcgcacctg atccaccaac ggttacataa
     5581 gaattgttga attctcctgc caacggataa tttgcgcctt gtgcgttagc tgccatatta
     5641 gctaatacga tcggggtcaa gaaagaagtg atgatattcg ctccatggat ccctacgatc
     5701 cacaatgcat ggatcaagaa ataaatcacc atgacaccaa gccatgtatt cgtcaactgc
     5761 gtaacaaacc caaatgggat agcaatcatt ttgaagatat ccgttcctag tgcaactaat
     5821 aatccattga tgatcatcac cacaaacgca atgacaaatg ttgggatcag agcagtgaaa
     5881 gaacgagaaa ctcctggcgg aacaacatct ggcattttta tgacccaatt tctcttgaca
     5941 cacatacaat acaattcgac cgcaataatc gacataataa tagctgtaaa gattcctgaa
     6001 gttcctaaac gttcgacaaa agagcccatc ctaataccat ttactacagt ttctgtctca
     6061 ttgatagaag caatcaagct gattttacca tcagtcatga tcaattcagg gatacacata
     6121 aagaatgcaa aaacagatag taatgccccc gtgagcggat taacatttaa tttttgttca
     6181 tctcgttcaa ttgacgttaa ttcataagca aacacaatgg caaagtaaat agataaaatc
     6241 cccatcgtca ttgtattgac gatcatgtac aagtcactaa ctttgaaaaa agttgcatta
     6301 aaaaatcctt ctaaaaatgg gaacggtaat ggcaaaatat ttaaaaccaa aaacatcgag
     6361 cctacaatcg tgaaaggaat agaagccatc cccgctgcca tgacagcccg aacgatttta
     6421 agttgcgcaa ttttccccat aggtcccatc aggtatttct gcaaaaaatc aaacattagt
     6481 tattctcctt tcctaaatat aacgcttttt catattgttt aatacggata tactgttggt
     6541 cttggttgag tgaaattttc tttaaacgat actgtataaa tgcactggca aaaataccgc
     6601 ttacaacaag aatgaaaaca aataactgtc ctccattgtt atttgccata aaaggataca
     6661 agaatgtaaa tcctgaggta aacaacactg gaatcactaa taaattaaag acactctgga
     6721 acaaaaaata ccactttgtc catggaacgt tcgtttggtg ttttcgataa agctttactt
     6781 gttctcctac tgcaggcaaa atagctaaaa tcagaatcat cggtaaacta cttcctaaac
     6841 ttttagctga tagaagcaga atcatccatt gcatgttcat gaaaaagaaa aaggcagtca
     6901 ggtaacgtac gattagataa cgattgaaat acatgttttt caaactaagt tgcttttttt
     6961 ctaattcgat tttccttttt actgtatcct gcacaatgat ccttcctttc tatttatttt
     7021 tttgatacaa ataattcatt tcctgtgcaa cttccaataa tgtcatcgtc gtcatcagat
     7081 gatcttgtgc atggaccatg ataatttcca tttctatttt tgtcccgctg gcatattctt
     7141 tcagaagttg agtttgtgac tgatgcgcat gaagaatctc ttcatttgct tgtcgcaact
     7201 tctcttctac ttcttcaaac atctctttac gcatcgagcg aaatgcttca tgtatcagtg
     7261 tccgtgcatt tccgctatgg aaaatgatat caaaagaagt cacttgtagt gtttcactgt
     7321 ttattttttc agccatctgt tttcctcctt attcgatcag tttgataaat ttactgctga
     7381 aattctcaaa aataggttca gctaagattt cctgctgtac ttttagctgg tcaactaacc
     7441 taacgattgc tttagtcacg accgtaattc cttcattttc gacataagaa ggcgaaacta
     7501 aaaaaacaaa atgaatttct tgatattgat cccactgcat cccagaagga atcaatgcaa
     7561 cggctacctt tgtttcgatt ccaactggaa cagcgggatg tgggactgcc actgtatcac
     7621 tgaatatgat ttgtcccata ttttcacgtt gctggatctg ctttcgcatt tctcttatat
     7681 atccttcttc ttcatttatg ctcagctttt gcagcaaatc atgaagaatt tcttcctttt
     7741 tcggttcgta cgtataagtc ttgaaaaatt tttcagacaa ctgttcggtg taaatctgct
     7801 ttttttgctg tagagaaaat gctggtatcg gtctatctga ggaatgatag ggtatagatt
     7861 ctccaactac ctttcgtatt ttacgaacat catcttcatt caaaaaaata ctgacatgta
     7921 aaactggaat tttgaaaaac attgtcgaaa gatcaataga agaaataatc aggtcgattc
     7981 cttttaaagt tgtttcattg atctcataat agccttttac acttaccact gtaatatttt
     8041 taccgaattc actgactacg cgattcttta atagttgggc actgccgtaa cccgttgcac
     8101 agataatcaa tgcatgtagt ttatgcgcag ctctttcttt ttccaatgcc gccatgagat
     8161 gcaaagccaa ataggcccat tcatcttcat tgatggtata gtttttcaaa gccggcatgt
     8221 tgctgaaatg cctttttact gcgtcaaaga ctttgggatt ttctttcttt atctcctcag
     8281 tcaatgggtt ctcaatgcga atacctcggc tcaatcgaat catcatcggt gacaaatgat
     8341 cgagtagtcc attgaaaagc tggagatcct ctactaatga aaaatgaaat tcctgagaca
     8401 atcttttcag aactttcgct aattcctttg atagttcctg atcattttga ttttctttgt
     8461 gattcgactt agccatgaga tgtaatgtca aataagcaat ttcttcttcg ggaaacgata
     8521 cacctgcgat tacctcaata cgttgaacga tccgctgcgc cacttgatac tctactcgct
     8581 tagaaacttc ttgttcgatg cctaattcct tgatttcaaa cccttctcgg agccgtttga
     8641 tccccaaagc caagtgtaaa gtcagattct gtatgataaa atccgaaact tttaacttag
     8701 cttctcttgt ttcatcgaga atgataatga ctagctcttc aaaactgatg tctgggaaaa
     8761 aacggctttg ccctaaatat tctttcaatg aatttccata atggtttcca aaaaaagtat
     8821 ccatgataaa atgccgtcga tttctttctt ccccttcgat ccatgtccct tgaccatgct
     8881 tagaaacgat ttttagtgaa tatggaatca atcgctcctt gatttcttgc atgtcctttg
     8941 tcaaactgga acgactgata taaagctctt ctgccaaatc atccataaaa agaatggcat
     9001 cttccaaaag caattggttc aatatatact tttgtcgatc ttcggactca taaaatcctt
     9061 gcggttctct agcatatcca ggtactattt cttgccgact caaaaacaag tcaaatgtca
     9121 gtctttgtaa aatatgcagt tggtagccat aacctggttt agcaatgatc ttacctccgt
     9181 ttttttctac taactctttg atccgctgca agtaattacg gattgtccga tcagataata
     9241 acaattcaga agctaaacgt tgactggtga catactcctt cttgtgatca atcaaatatc
     9301 tcaacagcaa tttttccttc tgtttcatta tcatccactc ctattccatc atatgttctc
     9361 ttcgatcacc tctgctaact tctggatgcc tgtagggatt gggacatacg cttggaaagg
     9421 gatactaaca acaggctttc caacagattc tcctaatttt ttgaatttat ccaaaaacat
     9481 cgtcgtttgc gggctgatca gaaataaatc aaaatcacta ttcttaatca tattatcgcc
     9541 ttctgtagct gaaactgcat ctacgagtac ctcttctctt ttttgcttaa agaaatccgt
     9601 tgtctttgag gccatcatcg aggatgacat tcctgccgca caaataatca atgctttttt
     9661 cataaatcaa tcactccttt tatttttata aaccaagcat atcttgtaag cgttttctat
     9721 tcaaaatata tatttccgta gcattgcgga aaaaatgtgt atcatatctt gaataataat
     9781 ttctggcacc gtgatatact agtgatagaa ttttttcagg aggggattta gtgagtatca
     9841 cagtgaatat tttgtataca agtacaaatg gacaagccag aaaatttgca gaagaaatgg
     9901 tcgaaagaaa cattgtcaaa gcgattcgtg cagaagaagg aaacgagaaa tatgactatt
     9961 actttccact agaagatcca gaatctcttt tactgatcga ccgttggaaa gatgaagaag
    10021 caatcgaaaa gcatcacaaa tccgaaatga tggctcaaat aaacgaactc cgtaaaaagt
    10081 ataaattaaa aatgaaagtc gaacgtttca ctgatttacc gtaaaaagat acacatggaa
    10141 tgatagcaat ttatcatcca tgtgtatctt cactttttcc ttatttttta ttcgatctca
    10201 tctaatgatt caaaccgtgt ttttctctta tttttcggat aagtatttca gtcaccccaa
    10261 tatacatgat tcctaaacat accccaagga tattctcaaa aatgcggatc gttactgctc
    10321 ctggaacccc gaataaactg gcagtgattg ccaaagcacc aaaagaatta aaaactgttt
    10381 tatatctata ggtagaacaa acacctaaag ctagaccacc taatataccg acccagttaa
    10441 aaggaataaa ttggctgata ccaataaata aaacacatcc aatcaatgag ccaatgattc
    10501 ggtctactgc tcgttctttg aaaccaatcg acatcaatcc atagctagat acaatagaag
    10561 aaaatgcaaa ccctgcccac atcaatcgct gaaaagggac atactctcct gcaacgatca
    10621 aaagactaat gccaaatgca tagatgagca tccaaatttt ttcctgcgaa tacatgcctt
    10681 tcaaaaaaat ctctttaaac aagcttttgt ctttgttctt ttctcgatgt tttctgtaaa
    10741 ggagaactga aaaccaacaa aaaaacaaaa ataataagct acttgtttgt gtaaagaaat
    10801 tcttgttcaa cgaatcttta ggtaacgaat aaacaataaa gagatatgaa aagccgtaca
    10861 aagaagcatt ccccattttg ggatcactag cggttgccat taatatgctg cttagtaaaa
    10921 taaaatgaat caacatgccc ataactgacc atttgatcag ctggacatac ggtgctacta
    10981 atagtgagaa catgacgatt gctaaaccta tgatcgaatg ggaaatctta taaccaaagt
    11041 caacaaaacg aatactcatc aacatacaga ataaaaccac agcgtatggt ttatgcttcg
    11101 attcaaataa tgttgtaatt ataatgataa aacaaatggc aaacaatacg atgaataatg
    11161 atcgtaaaaa caatgaacaa atatacatgc acttttcttt tttagaagct gcttctcgta
    11221 ttttttgttt taaaataaac ggatctagtt gtagagactg ataaaaatcc acctgttgtc
    11281 gtcctttctt tttactttta ttttttacag tcatctatga caggttgtaa atactatgga
    11341 acatcatatg gcgttgtcat tttcttgtca actatttttc ctattcatgt ttttataaaa
    11401 aaacatatcg ttttcttgta tgattaaatc accacaaaat aaacaactga gagaacgata
    11461 tgctttatga atggttctat caaaaaaatt ctgagatcaa tagacaaaaa tttgatgatt
    11521 acagaggtct cttacgaaac cttacagaag aaaaaaacat tgatcgttgc tgcagttctc
    11581 ttccctgctc catgtgtctg tagaaattgt ggttctgctg tggtagatgg aaataggaaa
    11641 ttaatggtgg tgaaaaacaa gaagaaggaa accattgtcc actttgaata atataatcat
    11701 atgccaatgg ttatacgcct aaaaaagcag cgctatacct gtaaaaactg tcgaagccat
    11761 tggaacgctc aaagttattt tatccgtcca aggcactcga tttcaaatca tgtcagacat
    11821 aaaatcactt ctttactgac tgaaaaagtg tcgttatttt ttatttcgaa aagttgttag
    11881 gtatctttga caaccgttat tcgcacattg aaagagttga gaagctattt accaaagtct
    11941 tccaaaagaa ttctgtcttg agtgttgagg gttgtgaatt tcgttctcac gtttccgttg
    12001 aggataaact gagttttatt tccgcggatg gcgagacaga aaaattaatc gagaaaattt
    12061 acctacacaa aaaattcctc gattaacaaa ctatttcctt agctgcacta atcctaaaga
    12121 aatagaattc ttagttacag acatgaacgc cacctacttt cagctcacca aacatgttct
    12181 accaaatgcg aaattggtga ttgatcgatt acttggtttt tccacgcctt tcaaggaggc
    12241 gtaaccttta ttcacgagtt ggttgaagct ttccgagaca aaagacccga cttgtttttc
    12301 tccttattga caaagagcca ttaaaactag agaaaaaatt acaactgatc gctgattatt
    12361 tgtagaaaga aaatcaaagt attacccaat aaacttgagt cattaggcaa aaaacactgc
    12421 gaaactcaag aatgattttc gcagtgtttt tgtatatttt ttattaacta gtattactac
    12481 aaaggtttat ttttcacttc tcaacgcagc acaaaattgt ctttacttat ttactggttg
    12541 ggttcaacct tctctactcc gatactaata tttgtttgta taccccttct aaaatcggta
    12601 gtttccattc aattgatggg tactagttga ttttaaaaac tatgcttatt caatttttta
    12661 ttttagctca tatatttcgt ttctttctaa attttatact gatgaaaaca agtagcacac
    12721 ccaatattga caatataaac tggctaatga tggtttctcc agtttttgga agatgcttat
    12781 ttccactgct tgttggattg tttcctctta tatctgagct atcagcaact tttctattct
    12841 cttttgttaa aaagatcgcc acttcttgtt ctttttcgat cttgaaagtt a
//
LOCUS       DACECR010000083        12891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.083, whole genome shotgun sequence.
ACCESSION   DACECR010000083 DACECR010000000
VERSION     DACECR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.083"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            224..658
                     /locus_tag="IWO17_002151"
     CDS             224..658
                     /locus_tag="IWO17_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0291011.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            759..1850
                     /locus_tag="IWO17_002152"
     CDS             759..1850
                     /locus_tag="IWO17_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ0291012.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            1880..2557
                     /locus_tag="IWO17_002153"
     CDS             1880..2557
                     /locus_tag="IWO17_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ0291013.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            2889..4184
                     /locus_tag="IWO17_002154"
     CDS             2889..4184
                     /locus_tag="IWO17_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0291014.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            4174..5634
                     /locus_tag="IWO17_002155"
     CDS             4174..5634
                     /locus_tag="IWO17_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0291015.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            5770..7266
                     /gene="glpK"
                     /locus_tag="IWO17_002156"
     CDS             5770..7266
                     /gene="glpK"
                     /locus_tag="IWO17_002156"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ0291016.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            7280..9103
                     /gene="glpO"
                     /locus_tag="IWO17_002157"
     CDS             7280..9103
                     /gene="glpO"
                     /locus_tag="IWO17_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ0291017.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            9104..9820
                     /locus_tag="IWO17_002158"
     CDS             9104..9820
                     /locus_tag="IWO17_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0291018.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            10126..12055
                     /locus_tag="IWO17_002159"
                     /pseudo
     CDS             10126..12055
                     /locus_tag="IWO17_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            12181..12735
                     /locus_tag="IWO17_002160"
     CDS             12181..12735
                     /locus_tag="IWO17_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ0291019.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
BASE COUNT         4316 a         2142 c         2914 g         3519 t
ORIGIN      
        1 gccattttca caaaattttt cttcatactc aaaactaaac gactttttca caacctctga
       61 ttcacggtgt tccagaagga gttttgtcga aaacaagccg aaattctttg aagatggcta
      121 cttctttttt tcaaatctag acagaggagg cggacattat tccgtgtttt ttgacaaaaa
      181 ataaaaatca tactatgata tgtacgccaa tggaggggat gaaatggaaa agccaattga
      241 aaaagcgtta gatgaatgtc ttttctttag cgtgaaaaag ctagatcgca tgttgaataa
      301 attagctgat gaagcgttta gaaggacagg tctggcacca acttatggtt ttattttatt
      361 gatattaaaa gaaaaagatg gaataccaca aaaagatatt gcacaaatgc tctattccgc
      421 cccatcaacg attgctcgtt ttgttgaaaa attagaatat aaaggatacg tcaaaacagt
      481 tagcgaaggt cgcttgtcat tagtttatct tacagaacaa ggacgccaat ttgctaagga
      541 agtcgatggt agctgggaag aacttcatca gtcatacaaa gcagttttgg gaaatgaagc
      601 aagtaaagaa ttagcccaga caatgaatga tgcaacagat aagttacaaa aaaaataaaa
      661 ttttttaagc aaattagttg catgtacaac taatcgagtg gtttaatgat agatgattct
      721 cgataggtta atcagagcga attatgggag gaatgacaat gtcacaattt gatcaatcaa
      781 ttactttacc ttcaggagtt caattgaaga accgattgat gctgtcgcct atgacgacac
      841 aactcagctt tttcaatgga gtgatcaccg aagatgagat cacttattat aaacaacgtt
      901 ctaaaggtct tggtgctgtt attactggtg ctgcaaatgt ccaagatatc ggaaaaggct
      961 ggccaggtga actcagcatc gctcatgatg aaatgattcc tcgattaagt gagctagcga
     1021 aagcaatcca aggagaaggc gcaaaagcga ttattcagat tttccacggt ggacgaatga
     1081 caagtcgagc aactttatct ggagaacagc ctgtatctgc aagtgcagtt gcagcaaaac
     1141 gtcctgatgc ggaaacacca cgagcaatga gtgaagaaga aatcgttgaa acaatccaag
     1201 catttggcaa agccacacgt agagcgattg aatcaggatt tgatggaatc gagttgcatg
     1261 gagcaaatac gtatttgatc caacaatttt tctctccgca ttcgaataga cgagaggatc
     1321 aatggggcgg tagcttggaa aatcgttatc gttttattga agaattatta acaacggttt
     1381 tctcagcggt tgaacaatat gctaaaaaac cattcatcgt agggtatcgt ttctcaccag
     1441 aagaatatga aacaccaggt atccgttttg aagacacgat ctggcttctt gaacgattac
     1501 gtgagtcaaa actagattat ctgcatgttt cattaaatat gtatgatcga atagctcgtt
     1561 ctgataaata caacgataaa tcgattcttg aagtggtcca taacacgatt caaggaaaga
     1621 tccctttagt tggtgtcggt gggatccgca atcgcgaaga tgtagagcag gtattgaaat
     1681 atgcagaact tgcagcaatt ggacagcaaa tgatcgtcga tcctgactgg gcagagaaat
     1741 tattggaaaa ccgtgaggat gaatttgtaa cgaaaccttt tgaagaagca tataaagagc
     1801 tttatttgcc aagcccatta tataatttct tgaaagcacg ttatcaataa cgcaattttt
     1861 atagaaagag gatgggaaaa tgaaagtatt gatcgtatta acaaatgtag aaaagtacga
     1921 aaagatcgag cgtcctacag gtttgtggtt aagtgaactg actcattttt atgatgtatt
     1981 agtaaaaaat gggatcgatg cagattttgt cagtcctaaa ggcggatatg tacctttaga
     2041 accgcaaagt gttttgaata tggatgaaat cgattgggcc tattatgaag atgaagattt
     2101 cagaaatcgg gccttgggaa ataccttacg tcctgatgat gtggatgcaa atgattatgc
     2161 agcggtttat tatgctggtg gccacggaac aatgtgggat tttccagaag caatagatct
     2221 tgggaaagtc gcttcacaaa tctatcgaaa tgaagggctg gtctcagctg tttgtcacgg
     2281 tgttgtagga ttgttgccag ttacagatga gtctggaaaa tctattttag ctggacgtac
     2341 agtgactggg ttcagtaatg aagaagagga agcaaatggt acaacgaatg aagtaccatt
     2401 cttagcagaa gatgctttga aagaaaaagg agcaaactat cagtctggag cagctttttc
     2461 agaagtggtt caaattgatg ggcggcttct tacagggcaa aatcctcaat ctgctcatca
     2521 attaggcgaa gcagttgttg aagcattaaa gaattaaaaa taaatgatgt gtctattcta
     2581 actattacat tacacacgct aaaactcgtc ttttcgttct tttctgtttc ctatttggac
     2641 agaggtacgt aaaaggcgag tttttttctt ttataagagt acattttttg aaacatctct
     2701 tcagaaatga tactcttcaa ttaggtccac tagtacatta ttgagaagca gacctagaac
     2761 acggtatgat ggagagggtt gattctgctg catcgctatg tatcataagg ttgaaagcga
     2821 gttatgaata ctgtgttttc tgattgagaa gttagcagta gaacacctta taagaaagag
     2881 gaatgcagat gaacattgta attgtcggcg ggtcgtttgc agggattcat gcggctattt
     2941 atcttcgcca attgatgcca acaagtgaaa tatatttgat tgaaaagcaa tcgaagttag
     3001 gatggattcc cagcgggttc aatttgatat taaaaggaaa ggttaccagt gaaaaacaac
     3061 tttcttggat cacgaaagaa gaactgactg accgctatcg aatccatgtt tacacagaaa
     3121 agacagtaat aggattgaaa gaaaaaaaag tcgttttagc aggaggaaaa aaattggatt
     3181 ttgatcgttt gattctagcg actggttcaa atcaaaagtt tcgaaatatt tctgcagaat
     3241 caacgcttat tcacccagtt aaaaagatac aaaacgtaac ggctcttcag caaaagatca
     3301 tgaatgcaaa gaaaattgca attattggtg caggacaagc aggaatcgaa atagctgaag
     3361 ggttggcatc aagaaaaaaa caaatccatc tttacgaaag ccgaaagtca attctttttc
     3421 gttatctaga tcctgaaatg acagaaccgt tagtaaagga aatgaaaaat caagggcttt
     3481 cacttttttt agaagaacaa gttgtgtctt tgaccgaaaa tgaaaccgct attgtagaga
     3541 cggaaaaaag aaaggagaac tatgatcttg ttttgctggc taatcattct cgtcccgaca
     3601 atcagatgtg ggaagaacaa ttaacattga atgatgatgg aacgatctgg gtagatgact
     3661 acttgcaaac gtctcaaaaa gacgtttatg cgattggcga tgcgatccaa gtaacttttc
     3721 gtccaacaaa tgaaaaaatg tatgtttctc tagtaaataa cgctgtccgt acagcacgaa
     3781 atgtcagcaa gacaattagt ggctcacaga caaaggatca gggaacctat cgtccagtcg
     3841 gcaatcagtg gtttggctat ttcttaggaa gcgtaggttt gacagaagaa gagagcattt
     3901 tttatcccca aaaaattgaa acaggatacg tagactacaa gcttacagca ataaatcaaa
     3961 gcaaagttaa actcaaattt ctatttgatt ctgaaaaaaa gattcttgga gcacagctcc
     4021 aaagcaaaaa agagatgttc caccttcttg accaactgac aattgcagta gaagaaggat
     4081 ggacgtttga acagttagaa gaacatgagc tatttttcca gccagaatac cgtgtgccag
     4141 atatggtgtg gtcaaaggtg gcgaatactg cctatgaaga ttgaagcatt attagaaaga
     4201 aaagagcagt tacagatcct gattttgcga aaccttgttc tgcaaggggg aacagcctct
     4261 atcaatggtt tgagagagca tgtccaactg tcaaaagcct cgtttgatca atatttggaa
     4321 gatattgaat tgattggccg aatgatggaa aaaaaagtag aagtacagcg aaatgagtat
     4381 caggcgctgc ttgtgttgga cgaagatgta agtttggaaa aaattctatt gtttttattg
     4441 caggaaagct tgaagtttaa gatgctcgtc tatttattag aacatcagca agtatccatc
     4501 gttcgattag cgacagcgtt taatatcagt gagtcttctg tttttcgaaa gatcaaagaa
     4561 ttgaaccagc tcttagagga atttggatta caaatcaaaa acggtcaatt atacggagaa
     4621 gagctacaga tacgatattt ttattatgaa ctattccaat atatcccgga agatcagcga
     4681 ccgctttttt tgcaaaatac accagaaaaa agaccgttca ttctaggatt ggaccgtgta
     4741 ttagaaacaa cgtttacggc atcagcagaa gcacagattg cttgctggct cgggattacg
     4801 aaaaaaagac tattgaatga aaaaagtacg tacgcaactt tgaaagaaaa gaaattactc
     4861 tatcagtcgg accggcttta tcaagcaatc gatccaatca tcgtgatgca tcttagtcga
     4921 acagcagcag aactgaatgt atacgaaaca atgatgtttt acagcttttt tgtttctttt
     4981 tcgatagtag acgaagaaat attttatcag tatgatttga cacggagcaa aaagttacca
     5041 accgctgttt tagatactta cattcgggaa acgatgttat ggcattatcg tcctcgtcgt
     5101 ttgaaaatca aagaagaaaa ggcagtaggc taccaaattt cccagatcaa caatgaatgg
     5161 tttttctttg ttggaaagat cgaagtctat gagcgtgatc ggcttttgga acagcagcag
     5221 aaaatgctcg ggcattcctt aacacagtta ctggcgaaat tacaggaaac agcggtgcag
     5281 caattgccga ggaaaagagc tgaagatagt gagcttagtt acctgatgat ccagtatgcc
     5341 aatgttcttt tgatgatcga tttttatatt gcaaaaccag tagtgattgg aatcgattta
     5401 gaaagtctgc cgatttatcg tattgctttt caacaatatc tgatcagaga actgagggga
     5461 atcggcggta ttgagattgg aagttatgaa gaaggtaaag aatatgattt ggtgatcaca
     5521 ttttgccaga gaaacaaaaa acagtgtgaa tattatctgt cagaatttgc gtcaccttac
     5581 gacattattc gattgaaaag aagaatagag acgctgaaaa aagaaaagaa ttgattaaaa
     5641 aatagcttca taataaaaaa ctgtcaaaaa aagaaaaaaa gaataaaatt ttgacagttt
     5701 ctttttttaa aacagtagta tgataagagt agttaaagaa agcgtttgca tataatggag
     5761 ggaatcacaa tgacagaatc aacgtatatc atggcgattg accaaggtac aactagctca
     5821 cgagcaatta tctttgacaa gaaaggaaga cacatcggca gttctcaaaa agagttcacg
     5881 caatatttcc cgcaagaaag ttgggtggaa catgatgcaa atgaaatctg gaattctgta
     5941 cagtcagtta ttgccggagc gttcatcgaa tcagggatca aaccaaatca gattgctggg
     6001 attggcataa cgaaccagcg tgaaacaaca gtaatatggg aaaaagatac aggacgtcca
     6061 atctatcatg cagttgtttg gcaatccaga cagtcagctg gaattgcaga taacttaaaa
     6121 aatgaaggct atcaagaatt tttccacgaa aaaacagggt tagtgatcga tgcctatttc
     6181 tcagcaacga agatacgctg gattcttgat catgtagaag gcgcgcaaga acgagctgaa
     6241 aaaggcgaat tgatgtttgg aacgatcgat tcttggttag tatggaaact gaccgatgga
     6301 caagcacatg ttactgatta ctcgaatgcc agtcggacga tgttgttcaa tatccatcag
     6361 ctagattggg atcaggagat tttagattta ctaaatattc ctcgagtgat gttaccaaaa
     6421 gtcacttcta actcagaagt ttatggttat acgcaaggtt atcattttta tggaagcgaa
     6481 gcaccaattt cagggatggc aggagaccag caagccgcat tattcggaca aatggcattc
     6541 gaaccaggca tggtcaagaa tacttatggt acaggttcat ttattgtcat gaataccggt
     6601 gaaaaaccgc aattatccaa aaataattta ttgacgacaa ttggttacgg cataaatgga
     6661 aaggtttact atgctttaga aggaagtatt tttgtggctg gttcttcgat tcaatggctg
     6721 agagatggac ttcaaatgtt gcaaaaagct agcgattcag aagacgcagc gaaacgctct
     6781 acaagcgaag atgaggttta tgttgttcct gcattcgttg ggctaggtgc tccttattgg
     6841 gatcaggcag cacgaggggc tatgtttggc ttgacacgtg gtacgacaaa agaagatatc
     6901 attaaagcta ctttacagtc gattgcttat caagtgagag atatcatcga tacgatgcaa
     6961 gatgacactg gaatcaagat tcctgtattg aaagtagatg gcggtgcagc aaacaatgaa
     7021 tatctgatgc aattccaaac agatatcttg aatgtaccga tccaacgagc agaaaacttg
     7081 gaaacaacgg cgctaggggc agcattttta gcaggattag ctgtcggtta ttggaaagat
     7141 acagacgaga tccgcgaatt ttatgaagct ggcaagctat ttgaagttca aatggaggaa
     7201 gagcgccgag aaaaacttta caacggttgg aaaaaagcag taaaagctac ccaagcattt
     7261 gaataaggag gaaatccata tgttttcaaa caagacaaga caagatagca ttcaaaaaat
     7321 gcagcaagaa gaattggatc tgttgatcat cggtggcgga atcactggtg ccggtgtggc
     7381 agtccagtca gcagcatcag gaatcaaaac aggattgatc gaaatgcagg attttgcaga
     7441 agggacttcc tctcgctcga ccaaacttgt tcatggcggt attcgttatc tgaagacatt
     7501 tgatgtggaa gtagtagctg acacagttgg tgaacgtgca gtcgtacaaa gtattgcccc
     7561 acacatccca aaaccagatc caatgctttt gccaatctat gagggcgaag gagcaacaac
     7621 cttcaatatg ttctcggtca aagtagcaat ggacctttat gacaaactgg caaatgtgac
     7681 aggaaccaaa tatgagaatt atacgctgac accagaagaa gtattggaaa gagaaccgtt
     7741 tttgaaaaaa gaagggctaa aaggcgcggg tgtctatcta gatttccgca acaatgatgc
     7801 ccgtttagtg attgataata tcaaaaaggc tgccgaagat ggggcttatc tagtaagtaa
     7861 aatgaaagcg gttggctttt tatatgatgg cgatcaaatc gttggcgtca aagctcgtga
     7921 cctgctgaca gatgaagtga tcgaaatcaa agcaaaatta gtgatcaata cgagtggtcc
     7981 ttgggtagat aaagtacgaa acttgaattt tacgcgtcca gtctctccta aaatgcgtcc
     8041 aactaaaggg atccatttag ttgtagatgc gaaaaaactg cctgtaccgc aacccacata
     8101 cttcgataca ggaaagcaag atgggcggat ggtttttgct attccaagag aaaacaagac
     8161 ttactttggt acgacagata cggattacca aggagatttt acggatccga aagtcacaca
     8221 agaagatgta gattatctat tggatgtgat caatcatcgc tatccagaag caaatatcac
     8281 attgaatgat atcgaagcaa gctgggcagg acttcgtcca ctgttgattg gtaattctgg
     8341 ttctgattac aatggtggag ataatggatc gatttcagac aagagcttca ataaagttgt
     8401 cgatacagta agtgaatata aggaaaacaa agtttctcgt gctgaagtag aagacgtgtt
     8461 gaaccatttg gaaaacagcc gtgatgaaaa agcaccttct acgatttcca gaggtagttc
     8521 tttagaaaga gaaccagatg gtttgctgac tttatctggt gggaaaatca ccgattaccg
     8581 taagatggca gaaggagctt tgcgattgat acgccaatta ttaaaagaag aatacggaat
     8641 agagacgaaa gaaatcgact ctaaaaaata tcagatttca ggtggaaact tcgatccaac
     8701 taaattagaa gaaacagtga cagaattagc aaaagaagga gtagcagccg gtttagagga
     8761 agaagatgct acttatatcg ctgattttta cgggactaat gcccgacgta tctttgaatt
     8821 agcaaaagaa atggcacctt atcctggttt gagtctcgct gagtcagctc ggttacgtta
     8881 tggtttagaa gaagaaatgg ttttggcccc aggcgattat ctcattcgtc gtacaaatca
     8941 tctgttgttt gaacgagatc agcttgatga gatcaagcaa cctgtgatcg atgcaattgc
     9001 tggatatttt gaatggacag aagaggagaa ggcgcaacag actaaacgtt tagaagcatt
     9061 gattgcagaa tcagatctgc gggaactaaa gggggagaaa taaatgaaca gtgatatgac
     9121 tcaaatcatg ggggagttta ttgggacact gatcctagtt ttgcttggag atggtgtgtg
     9181 tgcggctgtg aatttaaaca aaagtaaagc acaggcttct ggctggattg tcatcgcatt
     9241 tggttgggga ctcgctgtaa caatggctgt ctatatttct ggattcatgg gcccagctca
     9301 tttgaatcct gcagtctctc tagctatggc gatgacaggg gcaattagtt ggaatcttgt
     9361 cgttcctttc atcattgcgc aagttttagg agcttttgca ggggccattc ttgtttggct
     9421 ttcttatttg ccacattgga atgcaacgaa agatgaaagc gcgattttag gaacgtttgc
     9481 aacaggtcct gctatacgga attatccagc aaatgtgatc acagaattaa ttggtacatt
     9541 tgtattagtt cttgggttgc tagcttttgg acaaaacgaa tttgcacctg gaacgaacgt
     9601 attcgctgta ggtggattga tcttagccat cggtctttca ttaggagggc caacaggata
     9661 tgcaatcaac cctgctcggg actttggtcc tcgattagca catgcggtat tgccaatagc
     9721 taataaagga acttcagatt gggcatattc atgggtacca atcgcaggtc caatgatcgg
     9781 agcaattatc gcggtaggtg tctattcatt aatggtgtaa atctataaaa catactaaag
     9841 tttgacaacc aaaatgtgtc ttgctatagt taagtggaaa ataaataaga cctagaagaa
     9901 tggggtatgt tttgtgagtg tagctggtat ctaatcagta agtcgaatag ccaataaaca
     9961 aagtttattt ggtgtgctta tgaaacggta cctgtcagga aaacaatatc tttgtattct
    10021 aataaggata ggacatgact tttttaagct gtgttctatc cttttattta tctctaggcc
    10081 cttatttatt ttgggacagg tatctcttca agaaaaggag agaatatgga caacgaaatg
    10141 attagtcaat tacaatttga tcggatcaaa aaggaaatcc aagcaagagc aattggaaac
    10201 tatagcaaaa aacgaatcag cgatatgacg gtctcgacaa acctacaaac tgtcttggat
    10261 cgtcaagaag aaacaagaga agcacgcttg atcttagaaa gcagtcagca tgttccattt
    10321 atggggttgc cgaggatcga tgcactgaca gaacaagtca aaaaagggtt gattctgcag
    10381 ccaaccgatt tgatcgaata tgcggatttt cttagaagca gtcggatgat cacaaagttt
    10441 tttgataaga atcaatacca gacacccttg ttgtttgctt atagcaaaca tcttcctgat
    10501 ttgataaatg tagaagagtt gattgaccag aaaataaaaa ataataaagt cagtgacgac
    10561 gcgtcacgta atttgcgtaa agtacgcaaa caattacaac taatcgaaaa agaaatccag
    10621 tcaaaattac tgaaattctt gcgtcatccg aaaaacaaag aaatgatcca agaagcaatg
    10681 attgttcaaa aaggcgaata ctatacgatt cctatcaaag ccagctataa aaataaaata
    10741 gatggaacga ttatcgatga atcaaataaa agaacgacgg tttttattga accaactgtg
    10801 atttctaaat tgaatgaaca ctatcagttg ttaaaagctg aagaaatcag tgaagaatat
    10861 caagtcctgg cggcattgac tggtgctatc gcagaaaacg aagaagtgat cgatttattg
    10921 atcgaaacga tcacggtgtt agatatcatc tttgctcggg caaaatttag ccgagaaatc
    10981 aatggaagca cacctagaat caataaatcg gaacatatca ttatcaagca aggcagacat
    11041 ccatttttac ctgagcatgc ggtaccattg gatgttgaaa tcggtaaaga ctatcgaggc
    11101 ttgattatca ccggagcaaa tgccggaggc aaaacagtcg tgttgaagac agtcggttta
    11161 ttgactttga tggcgatgtt cggtatgcaa gttccggcaa aagaagggac agagcttgct
    11221 gtctttgatg agattttcgt cgatgtcggt gaccaccaaa atttagaaaa tgcgttgagt
    11281 acattttctg gtcatatgca aaacatcgct gctattttga aaaagataaa aaggaatacg
    11341 ttggtacttt tagatgagat tggcagtgga acagagccaa atgaaggggc tgcactagcg
    11401 atcgcaatca tggaatctat gtatgagcaa ggggcattga tcattgccac gacccattat
    11461 ggagaaatca agaaattcgc cggagatcat gaagattttg tgccggctgc tatggctttc
    11521 gatcgggaag cacttcgtcc taaatatcag ttgcgggttg gaagaaacag gagaaagcca
    11581 agctttgtgg attgctcata aaatggatat gtcgatgaaa ttgatccatc aagcagagcg
    11641 ttacttggcg gaaaaagcct atcggacaca gaaaaaagaa tttcttccta agacagcagt
    11701 tacaaatagg aaggagaata aaaaagaacg tcagctcttt gcaaaaggtg atcggatatt
    11761 tgtcaatgaa taccaaaaag aagcgttagt ttatgaagat ataggtgaag atacgattga
    11821 tgtatattta gacaaaaaaa taatccacgt gcctcgtcag cgtgttcgtc tggtgcgttc
    11881 agcagaagat ctgtacccga caggttacga tttagacagt ctgtttatcg attataaaac
    11941 acgtaaacgg cagcgtgacc tagagcgagg atcaaaaaaa gctcataaag tactagtaaa
    12001 agaaatgcgc aaacgacagg aagaaagaag agtaaacgat gaaaacagca agtgattatc
    12061 tcaaattagc tcataatatc agtgtctgta aagaaaaata aaaacagtga aatcattggt
    12121 tttattcccc gtgtaagcta aaataaaaag aaaagagtgc tgaggaggta gggcgataaa
    12181 atgaaattaa gcacagactg gcgtaaagaa atacaaacga ttccgaatct tttatcgata
    12241 ttccgtattt ttttgctgcc gatttatctt tattttgtgc tgagacagtc attttatata
    12301 gcaggagcgg tcattgttgt ctcaggtttg tctgattatc tagacggggt cattgctcgg
    12361 agatataatc aggtaacaga cttagggaag gtgcttgatc cgtttgcaga taaattgaca
    12421 caactttttc tgattttgtc tatggcatgg tatcgaccgt ggctttggct gttgtttggt
    12481 ctattcttga taaaagaagg ctttatgttt gtagctggat taatcgggct atctaaaaat
    12541 atcaaattaa gtggagcgaa gtggtatggc aaagtagcga ctgctgtcat ctatgttggc
    12601 atgatccttt tgctgctttt cccagaatta ccaacgctgt gggtgagagt catttttgcc
    12661 gtcatcactt acggtctttt acaatctttt gtcttgtatg ctgtcgagta ccgaaagatg
    12721 tttcaaagaa aataaaaaag gggtgtgacc tatgtcgcat tccttttttt gataaataga
    12781 ctcccagtag ctacttctcg ataataagcc aaccgaacca aaaacaggag aagttgtttt
    12841 tgaaattatt tcttgtgatt cggagttaaa tggtaggctc acaacctctt g
//
LOCUS       DACECR010000084        12843 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.084, whole genome shotgun sequence.
ACCESSION   DACECR010000084 DACECR010000000
VERSION     DACECR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.084"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(48..1112)
                     /locus_tag="IWO17_002161"
     CDS             complement(48..1112)
                     /locus_tag="IWO17_002161"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ0291020.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(1109..2194)
                     /locus_tag="IWO17_002162"
     CDS             complement(1109..2194)
                     /locus_tag="IWO17_002162"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ0291021.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(2207..3184)
                     /gene="mvaD"
                     /locus_tag="IWO17_002163"
     CDS             complement(2207..3184)
                     /gene="mvaD"
                     /locus_tag="IWO17_002163"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ0291022.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(3177..4121)
                     /gene="mvk"
                     /locus_tag="IWO17_002164"
     CDS             complement(3177..4121)
                     /gene="mvk"
                     /locus_tag="IWO17_002164"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ0291023.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            4443..5096
                     /locus_tag="IWO17_002165"
     CDS             4443..5096
                     /locus_tag="IWO17_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0291024.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            5181..6086
                     /locus_tag="IWO17_002166"
     CDS             5181..6086
                     /locus_tag="IWO17_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0291025.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(6088..6720)
                     /locus_tag="IWO17_002167"
     CDS             complement(6088..6720)
                     /locus_tag="IWO17_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0291026.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            7040..7564
                     /gene="infC"
                     /locus_tag="IWO17_002168"
     CDS             7040..7564
                     /gene="infC"
                     /locus_tag="IWO17_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ0291027.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            7636..7836
                     /gene="rpmI"
                     /locus_tag="IWO17_002169"
     CDS             7636..7836
                     /gene="rpmI"
                     /locus_tag="IWO17_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ0291028.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            7889..8248
                     /gene="rplT"
                     /locus_tag="IWO17_002170"
     CDS             7889..8248
                     /gene="rplT"
                     /locus_tag="IWO17_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ0291029.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            8500..9837
                     /locus_tag="IWO17_002171"
     CDS             8500..9837
                     /locus_tag="IWO17_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0291030.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            9885..12014
                     /locus_tag="IWO17_002172"
     CDS             9885..12014
                     /locus_tag="IWO17_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ0291031.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            11989..12678
                     /locus_tag="IWO17_002173"
     CDS             11989..12678
                     /locus_tag="IWO17_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291032.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
BASE COUNT         3897 a         2446 c         2729 g         3771 t
ORIGIN      
        1 tacttccgcc gtttgtacgt gtttagagcg tgagacaaaa gtgttcttta cttttgtccc
       61 acgctctttt ctgtgcgtct tccatacttc actaagtcga tccctcttgc ttcgcaccaa
      121 tccttcactg gtccggaaaa gatcagagat tgttggtgta gggccgcggt attcgttgcg
      181 ccgaccatag tataaagcag acgaagctct tcttgccatt gcttcatcag tataatagtt
      241 tcttctacgc cgtgattcat cagatgcgtc agtacggttc ctgaagtacc cacggcattt
      301 gcgccgagac aaagggcttt aaatatgtca taggcattgc ggatacctcc ggaggctagg
      361 atttccatag aagtgtctgc ttcatttgct tcaagcaatg aagagactgt cgattgcccc
      421 cagtcagcta aataactcag ttcccgcttg gagcgacggg cgttttcgat ctgggtgaag
      481 cttgttccgc ttcgaccgct gatatcaact gtatgaactc ctaaagcagc tagatcattg
      541 agcgtttctc ttgtcatgcc aaaaccgact tcttttacga tgagggggac gtcaatggct
      601 gtttgagttt cttgaataag tactttccaa ttggtaaaat cacggtctcc ctcaggcata
      661 accaactctt gaggtgcatt caaatgaatc tgtaaggcat ctgcgtgaaa caatcggata
      721 gcttctttgg ctctttcgac agaagtccct gcaccgatgt tggcaatgat tttcccgtga
      781 ggatattctt gtcgcataat cgtataagta tcggctaaag aagggacttt caaggcagcg
      841 ctgactgaac cagtggcaat catcaaatct gcttctcttg cgacgattgc aagatcttgg
      901 ttgatttttt ttgttttttc gcttccgcca gtcatcgcat tgatataaaa tgggctgctt
      961 agtgtaaacc caactgcctg tgtacttaga tcgacatcag aaactgctat ttgcggcaaa
     1021 gggttatgga tgatccgtac gaagtcaaat tcgttttttt gtttatcatg aaaggcttta
     1081 gctagtgaaa catgttcgtc ttttcgattc atcggttttc ctttctttga tcgctataga
     1141 catgtaacgg cagtggagtg atttctgctt tttcccatgc actcattaaa ggaagaatgc
     1201 cagatttctg gtcaacaatt acgattccgc aatcgccccc acctgcacca gaagattttg
     1261 cggctccttc atactgttca gctaaattac acaatttgtt caaagcaggc gtttcgatga
     1321 cgaccccagt gattgcagat aaatcatgca gtaattgtcg gttttttcga atcatctgtt
     1381 ggatcaacgt tacattattt tctttaaacc ctttgatcat ttcgttgaca cattctgtac
     1441 tattttttaa gaaaagctga taagccacca ttttatcttc tctcgaacgg tgaacttgat
     1501 cgaccaaatc agaagtagag gcagggctac ccgtccaacc aatcaataaa cgtaaatctt
     1561 caggagcaat caatggctca atggatagac ctggccaatc cagggcaagt aactcactga
     1621 tagaatgctg agtttcttgt tcttggagcc aaggatggtc gaaggttgag aaagcgatcc
     1681 agccaccata acagctagca gcgatgtcgc cgcaggaacc attatcttga actgctaaat
     1741 tggctagtgc tgcaatcttg aaaatctcca actgagataa attcaacgcg taaaaaacat
     1801 tcaaggcttt gaccgttgca acggtcacgg caccgctaga acccaatcca tatttccggc
     1861 catttgaact gtctaactca cttgttactt tcagatcata aaaggataaa agaatgtttt
     1921 tttcttgtgc atacttttca gtcaagcgaa tagcagcaag gatataatga aaaggatttt
     1981 cccgaatatc taaaactaat tctccgttgc gtcttgtcca acgtacaggc atcccactat
     2041 attgtgcaga ttggatactt ccgacttttc gtgcagattc tacagtgact gtcacgaatt
     2101 gatcgactgc agcgataact gctggatggc ctgtttcaac aactgcatat tctccggcaa
     2161 tatagagttt gcctggtgca gatacttcaa tcataaaaat ctccctttat tcaataatcg
     2221 caattcctgg accagcatgt gcagtaatta cttgttcttt gctaaataat ttggtaaatg
     2281 tttcttgtac ctcagataaa tggctgtttt ccactaaaac tttgacatta gggccagcat
     2341 ccatcgtaaa gtagcatgga atgccttgct ttctcaattg tcgcaccgca tccatcgctt
     2401 ttaagctgtc aggagaccag taagtgaatg gaggctgggc agctaaagtc gttccatgca
     2461 tttttagtcc gtttctttcc atcgtttctc cgagaagttg gaaatctttt gttttgattg
     2521 cttgtttcaa ttgatataga tccccttcaa cggaatctaa ccagccttga taaaaactgg
     2581 atgtttcgac tgtccgacgc atcccatttc tgctggacac ttcttttttc tggtcgttga
     2641 tcaaaacgaa aaccattgct aaatcgtctt cgaaagaatc ggaagggact ggcttagcgt
     2701 aagaacttaa gtcgtcatgg cctttttccc attcgacgaa accaccgaaa atacttcggc
     2761 aagctgaacc agacccgcgt cgagcaaatc ttgaaagaga gagatcatct aatccaagct
     2821 taagagcagt attgcaagcg cctgctaaag cagctagccc gctggcagag gaggctaatc
     2881 cagctgcggt aggaacgaaa ttctgactga ttactgaagc ttttagtgag aggccggctt
     2941 ctttacgaaa aaggtcaaga aactgactga ctttttttgt tgctttttcg tcttgcaagg
     3001 tgccatctag ataaaattca tctaccatat agctatcaga aaagatgact tctgtctctg
     3061 tataaaatgc atccaatgtt aacgaaaggc tgttgttcat tggaaggatg agttcttcat
     3121 ttttcttacc ccaatatttg attagagcaa tgttggtata tgcgcgtgct ttgcctttaa
     3181 acataggtat gtactcctaa tccttgtatc caagtttcag cagcacccgc atcttcaagt
     3241 gcttgactga tttcttgggc ttttgttttt gtttgtgcta atgcaatcat acagccaccg
     3301 cgtccaccgc cagttaattt ggcacccaat gccccggtat cttttgctgt ttggatcaat
     3361 agattaagaa aatcattgct gattgtcagc ttttccagaa gtgattgtga ttcatccatt
     3421 gtctgtgcca acgtttctgg tgaattttca atgattgctt gttttgcttg cttcgtcaag
     3481 tatcctaatt tttggatcat ttgtccagtt tcatgaggct gggtttcaaa gaggtgagcg
     3541 acatctttga cggcttctct tgtttgtcct ttgattcctg tatcagcgac aatcaaaaaa
     3601 gcatcgatgt tcaaagaaaa gtagtcgaaa ggatgccctt ttgtaaaata aatcggctgc
     3661 aagctgctag tagcggctgc atcgattcca ctaggattac catgggcgat tttttccgaa
     3721 agctggacat tttctaatag tatttcacga gacaaaggaa atgctaagta atcataaaaa
     3781 gcacgagtga ctgctgtggc gactgcagcg cttgatccca ttccacgttc agcaggaatc
     3841 gtgctttcaa tggtcagttg caagtttttt tggatgtcat gctgtttttt taagtgaatg
     3901 aataattttt tgatattttt taatgcatga ggagcttcgg ctaaatttcc tgaatagtag
     3961 gaagaaacca gttgatcgtc cattgcatcc agctcttcaa ggaatgcggt gacttttgtt
     4021 gcatagaaag gaaaggcaat cgctggttct ccataaacaa ccgcgtgctc gcccatcaat
     4081 atgatctttc cgcttgattc tccttggcca tagtttgcca taatctaact ccttctcacg
     4141 taaaagtgat ttttaagcaa aaaataaagc gcccaaaacg agttgcgctg cttcacgaat
     4201 ttttttgtcc actaagggtc tcaaaaaaca ttcctccttt attttactaa aaccatctca
     4261 gaagtaaaac taagaatacg ttttctaaag aaaaaacact tttttcttaa gaattttttg
     4321 actttttttc ttctttgact agaaaaaaat acaattgaca ctactagata ttgggggtat
     4381 atttaaaaag gttggagtta aacacaatat ttagtgatga agtgagctgg aggtaaatga
     4441 gcgtgatcgt tttagcaggt acaattggtg ccggaaaatc aagtttaaca gaaatgatgg
     4501 ctgaacactt tgatagtcag gcattttatg aatcgattga tgataatgaa gtcttgccct
     4561 tattttatgc aaaccctgaa cagtatgctt ttttgctgca aatttatttt ttgaataaac
     4621 gttttgcaag catcaaacaa gcaatgaaag atgacaataa tgtattggac cgttcgattt
     4681 atgaagattc actgcttttc catctaaatg cagatctagg aagagcaaca gaaacagaag
     4741 ttcgtgtgta tgatgaactg ctggaaaaca tgatggaaga acttccctat gctgcccata
     4801 aaaaacatcc ggatttatta gttcatattc gtgtatcttt tgatacgatg ttggagcgta
     4861 tcgaaaaacg tggtcgttcg tatgaacaat tgtcctttga tccaagtcta tacgattatt
     4921 acaaagaatt gaacagacgt tacgatcaat ggtatgaaga atacaaagaa agtccaaaaa
     4981 tccaaatcga tggagaccgc tataattttg tggaagatcc tcaagcaaaa gaagaagtct
     5041 tgaaaatgat cgaagaaaaa ttagcagaga ttcgccaaac caaagtagcc atttgattta
     5101 cagttgctca aataaagggt aaacctttat ttgagcgact tttttcttta tactggaaac
     5161 aaatgtcagg aggatagagt atgcgggaaa aacactattt taatcaagac cgtaattttt
     5221 ggatcaatgt acagcaaaac gatgtgctag ctgttaaaaa cttaaaagaa gaatatggca
     5281 ttagtgacga gatgctgact tattctctag ataaaaatga acgggcgcgc gtagagtacg
     5341 attcttttga tgaagcgtta ctgcttgtat caaatgttcc gcaccagcaa aaagtcgata
     5401 atcattatga aactagtcct atcgctttta ttttaaaaga agacggtttg tttacattta
     5461 ccacgccgaa taccgagtac gtgattcgac taattcgatc gcttttggaa cgaatgccag
     5521 acatgagtgt atacagttta ttgtttcgta cattgttttt gatttctgat tccttttttc
     5581 ctttaatcga ggaagtgaat agtgaacgtc agcggcttaa tctgaaatta agagaaaaaa
     5641 caacgaacaa gaatctgctg caactttcag atttagagat cggtttggtt tacttagtta
     5701 caggaacaaa acaaaatgta gtacttttag aacaaatcaa ggcattggct atttatcgca
     5761 agttgtcaga aaaggaaaaa gaagagttgg acgatgcgct gatcgaagca cgacaagcag
     5821 ttgaaatgac gaacttagct tcgcagattt tagaccagct ctctggcact tataataatt
     5881 tgctgaataa caacttgaat gacacgatga aatttttgac ggtctggtcg ctgatcttga
     5941 ccgttccaac tattgtgaca ggattcttcg gaatgaatct ccagttaccc tttacccatt
     6001 ccgttttcgg atggggaatc gccttgatca tcagtttggt cttatctatt tggatgctta
     6061 ttgcactgtg gcggcggatt cgttgatcta gtctttgatc tgcaaaccaa gggcttgggc
     6121 gatcacaagt gcttgctctt ggttgacgat tagtcccgcc aaagaatctt gagataaagc
     6181 gattgctgtg aagaaattag tcgtaaagtc taatcccttc agagaagttc gaaaccagtt
     6241 cgaaccggtc aactgattgt cagacaaaaa aaggtttttc caagtgacct cataaaattc
     6301 gctgtcttct aattgacaat gatcgaagga gacagctttc aagtttgtac tactaaagga
     6361 agccatcgaa gcaatacaat cagtgaaagt acagtctcga agataacttt cggcaaaatt
     6421 cgtacctgtt aatttacatt gatgaaacac gacttggtgg aaactgccac caatccactc
     6481 cagattggaa aaatcacatt tttcaaaaat gacattactg cattcaaaat gacgtaaaac
     6541 tgttttttgc atcgtaagtt tttggatatg actctctctt aataccatat ttttggcatc
     6601 gagatagctt tggtcacccc cgttgatcag aagaccagag aagatctcct catcttctaa
     6661 ataaaactct ccctttgtaa gaagaggaag agaaggtgga ataggttcga tttttttcat
     6721 gtgggttcac ctcgtttttc tttagtatag caaagaagcg ggaatcactg ctagaaagtc
     6781 tgctaatcta atcattttca gaaaaggaag acaaactgta tgaaaaacat tgacaaaaag
     6841 attagttttt gctatactat gatggttgag aaataaaagc agaagcaccc gcttctcgcc
     6901 ttagttgata atgtcactgg gctagatagt tttcactggt tctattaggt gaagtacgcg
     6961 cgggttctgt aagagaactc gtttttttat tgccgtttat tcggtaagct tttctttctc
     7021 tcataatatt tggaggtgaa tgaccatagc aaaggatatg atggttaacg acggcattcg
     7081 tgcacgagag ttacgattga tcggttcaga tggtgagcaa ttaggagtta agacaaaagc
     7141 agaagcattg aatattgcag aacaagcaaa cttggatctt gtgttagttg cccctggtgc
     7201 gaaaccacca gttgcgcgaa tcatggacta cggtaaatac cgtttcgaac aacaaaagaa
     7261 agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta aaagaagttc gcttaagtcc
     7321 tacgatcgac ttgaatgact tcaatacaaa acttcgtaat gcacgtaagt tcttggaaaa
     7381 gggcgataaa gtgaaagctt ctatccgttt caaaggccgt gccattaccc ataaagaaat
     7441 tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg gatattgcaa cagtggaaca
     7501 aaaagcgaaa atggacggac gcagcatgtt cttgacgctg gcaccgaaaa acgacagtaa
     7561 gtaatttgaa tgactaactg gctgttggac agccgatagt cggataacga aagattttga
     7621 ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg gatcagcaaa acgtttcaaa
     7681 cgtactggta aaggcggatt gaaacgcttc cgcgcgttta caagtcaccg tttccacggc
     7741 aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca tggtttctag tggcgacttc
     7801 aaacgtattc gccaacaact agcaaaaatg aaataagaag tacaaataac agaatctgaa
     7861 gatttatttt tattaggagg aattatacat ggcacgtgtt aaaggtggaa cagtaactcg
     7921 taaacgtcgt aaaagagtgc taaaattagc aaaaggttac tacggttcaa aacacacttt
     7981 atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac tacgcatatc gcgatcgtcg
     8041 tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt atcaacgcag cagctcgtat
     8101 gaatggcttg agctactcaa aattaatgca cggtttgaaa gttgctgaaa tcgacatcaa
     8161 ccgtaaaatg ttagctgact tggctgttaa cgatgcagca gcatttactg cattggctga
     8221 ccaagcaaaa gaagctttgg ctaaataagc taaacaatta taaagaggaa agaagataac
     8281 gaaaagttat gttctttcct ctttttttat ttacaatcct ccatcaaatg aaacaggaca
     8341 ataacgtata agtctaaaat agcataatta aggtttttta attgaaaact gaacatttat
     8401 ggtcaccctt taatgaaagc gctaactaca taagttagaa tgagtttgac tgatatatta
     8461 gtttgcttat cagaaaaaca aaatggacgg aggaataaca tgagtgatat tattattggt
     8521 attttgttag ttatctcatt ctttttcatg gtctggtact gtgtaaaagg ctacaacttg
     8581 atggtcggct ttgccatcat ggctactgta tggatgggat tagcgctagt cggaaacacg
     8641 ttttcaccaa acccggcgat ggaagggcaa ggagtaattg atgtgttgac gcatatctat
     8701 acgacggggc cagctgagta cgccaaatcg atcttggtaa atgtcttctt tggtgccttt
     8761 ttcgggcgtg ttctagtaga tagcggaatc gcagctaccc tgattcgaaa ggtagttgaa
     8821 cttggagggg acaagccaag aatcacgatg agcttactat gtattgtaac cgctgttata
     8881 ttcatgtcga tgacaggaat cggaccagtg atttctattg cagttattgt gttgccaatc
     8941 ttgatgtcat taggtatttc agtaccggtt gcactgttct catttatggg ctcgattatg
     9001 gctggaatct ttgctaatat cgtcaatttc aaacaatatc aaacgattta tgctggtttc
     9061 aatccagcgg ctgaaagcta tacttataat gactatttcc aaattggtat gattgggatg
     9121 attgtaagct tagttgtggt actaacggtc gctaatatat caatgaacaa aaagaaacgc
     9181 tatgcaatgg cagcaaacgt accagctgaa ggtggcgacg caccaatgat ctcatggtta
     9241 gccgtattat taccagtttt aggggtcgtt cttttagatc ttccaatcat cttaggtttt
     9301 attctagcag gtatctgggc attgctgttt actggaaaat tgcgcggagg ttataaagaa
     9361 atctgtcgtc agtttgcgaa attatttaca gatggcgcag tagatgttgc gccaatggtt
     9421 ggtttcttga tgaccttagc gatgttcaat aattcagctg cgtatgcttc tccttatttt
     9481 tcagctattt ttggagattg gataccgcaa tcaccattag tcttggcaat cgtatttgct
     9541 atattgacac cactaggatt tttccgcggt ccaatgaatc tagtcggttc aggctcagct
     9601 attttagctg tcgtattagc tgttaatcca acgatgtcgc cagccttttt gttcccgtta
     9661 tttgcaatca caacgattgc tcctcagcat ttagatatta cacaatcttg ggttgcttgg
     9721 ggattaggtt atacaaaagt aacttctcga gaatatatga agaaatccat cccaactggt
     9781 tggatcatcg gagcgatctt atgcctgatc acgttcttat tgtacgggaa cgcctagtaa
     9841 acaaaacgtc gataaaaacg attagatgaa tggaggaaaa gaaaatgaaa cgttttgtgc
     9901 gaatgggaat tgatgttggc ggtacacata cgaaagcagt cgcaatcgat aatgcgacgc
     9961 acgaaatcat cggaaaatct tcagtgaaaa caacccacga tgatgtccgt ggagtagccg
    10021 caggtgtcgt tcagtctttt caaaattgtt tgctggaaaa taatatcagt ccagaagatg
    10081 ttgtgtttgt tgcacattct accacacaag cgactaatgc actgattgaa ggagacgtag
    10141 caaaagtcgg cgtaatcggg atggcaaaag gtggattaga aggtttttta gcaaaacgac
    10201 aaactagatt gaacgatatc gatttaggga ataagaaaaa aatagagatt gtcaatgcat
    10261 tcttacctgt caaacactta aatgtagacc gtgtttcaga aacgatttct tctttagaaa
    10321 gagaaagagc ggaggtcttg gtttcttcta tggcatttgg agtagataat ggggaaccag
    10381 aacgagttgt ttatgaagca gcatcggtaa aatcgatccc tacaacgatg gcttctgaca
    10441 tcacaaaatt atatggattg acaagaagaa ctcggacagc agcaatcaat gcatctattt
    10501 taccaaaaat gctagatacg gcaacctcta cagaagattc cgtacgagaa gcaggggtca
    10561 atgtttcttt gatgattatg cgcggagacg gcggagtaat ggaaatcaat gaaatgaaaa
    10621 aacgacctgt tttaaccatg ctttccggtc cagcagcatc agtcatgggc tctttgatgt
    10681 acttgagagc gtctaatggt gtttattttg aagtaggcgg aacaacaacc aatatcgggg
    10741 tcattaaaaa tggacgtcca gcaatcgatt attcaattgt aggtggccac ccaacatata
    10801 tctcttcatt agatgttcgg gttctaggtg tggcaggcgg ttcgatggta cgggctaatc
    10861 agtcaggaat catcgatgtc ggtccacgtt ctgcacatat tgccggtcta gattatgcag
    10921 tatttacgga gacagaaaaa atcaaaggtc ctaaggttga atttttctct cctaaagaag
    10981 gagaccctgc tgattacgtc aaggtagtga tggaagacgg agaagaagtg actattacaa
    11041 atacatgtgc agccaatgtc cttggattag tgcaagaaga acatttttct tatggaaatg
    11101 tcccatctgc acgaaaagcc atccaagcat tagctgatta ttgtcatacg acggttgaag
    11161 acatcgcaga acagatcatg gaaaaatcct atgctaagat tgaaccggtg attttagaat
    11221 tggcagacaa gtatcacttg gagaaagatc agatttctct tgtaggtgta gggggcggcg
    11281 ctgcctcatt gattacttac tttagtaata aaatgggtgt gaaatattcg attcctgaaa
    11341 acgccgaagt gatttcttct attggtgtag cacttgcaat ggtccgagat gttgtagaac
    11401 gtatcatccc ttcgccaagt aaagaagata tacgatcgtt gaaaaatgaa gcgatgaata
    11461 aagcgattga atcaggcgct acaccagaat cgatcgaagt ccacgtagaa atcgatccac
    11521 agacttcaaa agtaacggct atcgctactg gttctacgga agtgaaagca accgatctaa
    11581 ccaaggagat cacaacagaa gaagcactag agcttgctgc agaggatatg cgattaaata
    11641 aaaatgaagt ttgcttattg gaaaatacac ctttctttta tgtatgcggc gaacagaatc
    11701 gttcaaaaaa tgctggaagt cttcggatca tcgatcaaaa aggatttatc aaggtccaac
    11761 gtggacatgc ttcatgtatg aaaacaactg cagccaatta tatgacagct gtcgaacaac
    11821 tttgggaaga tatggcagtt tatcaaactg aattgattgc ccgaccagaa ttttatctct
    11881 gcttaggagc aagagtctct gattttacag cgacggatct ggaacaacta cagcttttga
    11941 tggatctgga agtatctact atggaaccgg aggaagaagt gatcgtcgtt gccggaaata
    12001 tcaaacaaac ataagttaga agagttacta aacgaattac aacagatccc cgaagaaatt
    12061 tggggattct accagtttcg gcgagattta ttttggaaaa agattccttt gtccaagcag
    12121 aaaattctta tacaacaatc aatcgattgt ggtatcgaaa cggcatgtag catcaaaaaa
    12181 aagtaccctt ttgccgatgt aggagaaatt tgtgaacaaa tggctgttcc aatcgtttcc
    12241 tgtgaatcag agcaaataaa cgaacgaatc acttttgcaa cttatgcaga agatgaaggg
    12301 attcgattga tgacggagcc gttagaaaaa ttaaaatgtt caggactagc ttcgatctct
    12361 aaagaaacag cgcaagcttt gattattgga catgaactat ttcaccacat cgaagcttct
    12421 gttaagggaa tttatacgca aagcgaaaaa attgtgctgt ggcggttgcc tttttataca
    12481 catcaatcaa acattcgtgc attgagcgag atagcagcga tgagtttttc aaaagagatg
    12541 aatcaaagcg atttttcgcc ttatgtatta gaggcagtct tactttggcc atataatgaa
    12601 attcgtagcc aagggatctt agaagaaata aaaaaaatag aaaaaagatg tacggagtat
    12661 gatttagatt acaaataaag agactgtgac aaaagtcgtg tcacagtcct ttttttccga
    12721 ataaacgatg ttctcctgtc aactcctcgg tcacaagtca aaatattcaa caaacatgag
    12781 aagctgtttt ttgaaattgt tccttgtgac tcggagatga acggcaggct cacgacctct
    12841 tgg
//
LOCUS       DACECR010000085        12739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.085, whole genome shotgun sequence.
ACCESSION   DACECR010000085 DACECR010000000
VERSION     DACECR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.085"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWO17_002174"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWO17_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0291033.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IWO17_002175"
     CDS             complement(322..891)
                     /locus_tag="IWO17_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ0291034.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IWO17_002176"
     CDS             1077..1979
                     /locus_tag="IWO17_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0291035.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWO17_002177"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWO17_002177"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ0291036.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWO17_002178"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWO17_002178"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ0291037.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWO17_002179"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWO17_002179"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ0291038.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IWO17_002180"
     CDS             8547..8732
                     /locus_tag="IWO17_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ0291039.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IWO17_002181"
     CDS             complement(8794..9990)
                     /locus_tag="IWO17_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0291040.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IWO17_002182"
     CDS             complement(10160..11596)
                     /locus_tag="IWO17_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0291041.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IWO17_002183"
     CDS             11889..12698
                     /locus_tag="IWO17_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0291042.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4013 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaatttt
//
LOCUS       DACECR010000086        12670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.086, whole genome shotgun sequence.
ACCESSION   DACECR010000086 DACECR010000000
VERSION     DACECR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.086"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWO17_002184"
     CDS             6..305
                     /locus_tag="IWO17_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0291043.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWO17_002185"
     CDS             378..1739
                     /locus_tag="IWO17_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0291044.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWO17_002186"
     CDS             1761..2021
                     /locus_tag="IWO17_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291045.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWO17_002187"
     CDS             2035..2787
                     /locus_tag="IWO17_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ0291046.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWO17_002188"
     CDS             complement(2881..3486)
                     /locus_tag="IWO17_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0291047.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWO17_002189"
     CDS             complement(3539..4513)
                     /locus_tag="IWO17_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ0291048.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWO17_002190"
     CDS             complement(4891..5670)
                     /locus_tag="IWO17_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0291049.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO17_002191"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO17_002191"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0291050.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWO17_002192"
     CDS             7161..8558
                     /locus_tag="IWO17_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0291051.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWO17_002193"
     CDS             8639..9079
                     /locus_tag="IWO17_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0291052.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWO17_002194"
     CDS             9227..9553
                     /locus_tag="IWO17_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0291053.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWO17_002195"
     CDS             9554..10303
                     /locus_tag="IWO17_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291054.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWO17_002196"
     CDS             complement(10383..10838)
                     /locus_tag="IWO17_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0291055.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IWO17_002197"
     CDS             complement(11265..11540)
                     /locus_tag="IWO17_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291056.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IWO17_002198"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IWO17_002198"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ0291057.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4055 a         2175 c         2587 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACECR010000087        12369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.087, whole genome shotgun sequence.
ACCESSION   DACECR010000087 DACECR010000000
VERSION     DACECR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.087"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="IWO17_002199"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="IWO17_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ0291058.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="IWO17_002200"
     CDS             complement(738..1460)
                     /locus_tag="IWO17_002200"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ0291059.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="IWO17_002201"
     CDS             complement(1596..2477)
                     /locus_tag="IWO17_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ0291060.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IWO17_002202"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IWO17_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ0291061.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="IWO17_002203"
     CDS             3717..4196
                     /locus_tag="IWO17_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ0291062.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="IWO17_002204"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="IWO17_002204"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ0291063.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="IWO17_002205"
     CDS             5497..5625
                     /locus_tag="IWO17_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0291064.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5629..6174
                     /locus_tag="IWO17_002206"
     CDS             5629..6174
                     /locus_tag="IWO17_002206"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0291065.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6629..8320)
                     /locus_tag="IWO17_002207"
     CDS             complement(6629..8320)
                     /locus_tag="IWO17_002207"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ0291066.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="IWO17_002208"
     CDS             complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="IWO17_002208"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ0291067.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9802..10821)
                     /gene="argF"
                     /locus_tag="IWO17_002209"
     CDS             complement(9802..10821)
                     /gene="argF"
                     /locus_tag="IWO17_002209"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ0291068.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="IWO17_002210"
     CDS             complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="IWO17_002210"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ0291069.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3407 a         2546 c         2214 g         4202 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttcacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaatcca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacaaga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
     6481 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
     6541 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
     6601 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
     6661 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
     6721 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
     6781 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
     6841 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
     6901 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
     6961 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
     7021 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
     7081 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
     7141 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
     7201 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
     7261 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
     7321 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
     7381 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
     7441 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
     7501 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
     7561 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
     7621 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
     7681 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
     7741 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
     7801 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
     7861 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
     7921 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
     7981 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact acggtttgca
     8041 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
     8101 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
     8161 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
     8221 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
     8281 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
     8341 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
     8401 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
     8461 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
     8521 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
     8581 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
     8641 ctagtcgttt cataaaaaaa aaatccagat ggcctaaagg agaagaaata ccacctggat
     8701 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
     8761 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
     8821 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
     8881 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
     8941 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
     9001 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
     9061 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
     9121 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
     9181 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
     9241 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
     9301 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
     9361 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
     9421 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
     9481 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
     9541 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
     9601 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
     9661 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
     9721 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
     9781 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
     9841 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
     9901 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
     9961 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    10021 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    10081 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    10141 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    10201 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    10261 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    10321 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    10381 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    10441 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    10501 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    10561 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    10621 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    10681 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    10741 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    10801 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    10861 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    10921 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    10981 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    11041 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    11101 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    11161 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    11221 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    11281 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    11341 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    11401 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    11461 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    11521 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    11581 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    11641 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    11701 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    11761 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    11821 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    11881 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    11941 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    12001 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    12061 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    12121 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    12181 atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca
    12241 ttaatattcg ataattaaat aattaaacgt ttttctattt ttaagcgtaa tttattcatt
    12301 ttttattctg tatacacttt caaaaaaata tttattcatt gaaaaaattt ttttgaataa
    12361 attaaaaaa
//
LOCUS       DACECR010000088        12171 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.088, whole genome shotgun sequence.
ACCESSION   DACECR010000088 DACECR010000000
VERSION     DACECR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.088"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            592..1920
                     /locus_tag="IWO17_002211"
     CDS             592..1920
                     /locus_tag="IWO17_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0291070.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="IWO17_002212"
     CDS             2122..2955
                     /locus_tag="IWO17_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ0291071.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="IWO17_002213"
     CDS             2971..3708
                     /locus_tag="IWO17_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ0291072.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="IWO17_002214"
     CDS             complement(3863..4831)
                     /locus_tag="IWO17_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ0291073.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="IWO17_002215"
     CDS             5063..5896
                     /locus_tag="IWO17_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ0291074.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="IWO17_002216"
     CDS             5930..6769
                     /locus_tag="IWO17_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0291075.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="IWO17_002217"
     CDS             6795..7355
                     /locus_tag="IWO17_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0291076.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="IWO17_002218"
     CDS             7554..9275
                     /locus_tag="IWO17_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ0291077.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="IWO17_002219"
     CDS             9440..10582
                     /locus_tag="IWO17_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0291078.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="IWO17_002220"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="IWO17_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ0291079.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4148 a         2042 c         2527 g         3454 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt g
//
LOCUS       DACECR010000089        11757 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.089, whole genome shotgun sequence.
ACCESSION   DACECR010000089 DACECR010000000
VERSION     DACECR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.089"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(258..2333)
                     /locus_tag="IWO17_002221"
     CDS             complement(258..2333)
                     /locus_tag="IWO17_002221"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ0291080.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2335..3252)
                     /gene="glyQ"
                     /locus_tag="IWO17_002222"
     CDS             complement(2335..3252)
                     /gene="glyQ"
                     /locus_tag="IWO17_002222"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0291081.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3636..4448)
                     /gene="recO"
                     /locus_tag="IWO17_002223"
     CDS             complement(3636..4448)
                     /gene="recO"
                     /locus_tag="IWO17_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ0291082.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4591..5490)
                     /locus_tag="IWO17_002224"
     CDS             complement(4591..5490)
                     /locus_tag="IWO17_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ0291083.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5505..5906)
                     /locus_tag="IWO17_002225"
     CDS             complement(5505..5906)
                     /locus_tag="IWO17_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ0291084.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5884..6360)
                     /gene="ybeY"
                     /locus_tag="IWO17_002226"
     CDS             complement(5884..6360)
                     /gene="ybeY"
                     /locus_tag="IWO17_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ0291085.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6377..8569)
                     /locus_tag="IWO17_002227"
     CDS             complement(6377..8569)
                     /locus_tag="IWO17_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ0291086.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8593..9564)
                     /locus_tag="IWO17_002228"
     CDS             complement(8593..9564)
                     /locus_tag="IWO17_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ0291087.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10269..11648
                     /gene="brnQ"
                     /locus_tag="IWO17_002229"
     CDS             10269..11648
                     /gene="brnQ"
                     /locus_tag="IWO17_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0291088.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3196 a         2451 c         2138 g         3972 t
ORIGIN      
        1 aattcttgac gcaaccactg ttttcttgac tagggcaatt tacgattaaa aagtaaataa
       61 aacattaata agataacaat taatcgattt tatttttatt tccttaattt tcataggtat
      121 tttcaaaagt tacacaaaaa ataaaataac aaaaaaaatt aacattgaat taataatagt
      181 tatttatatt tataacaaat aaaccagaga aacagcctat tttgtgcctg ttctctggtt
      241 tatttacata ttagaaatta ttttgtgatg atttgatcta aacttgctag tgacaaagcc
      301 atggcagcaa tttgacttaa ttgcgttaat cggttcttac gtactgctgg ttcttctgcc
      361 atgaccatcg tctgatcaaa ataattttcg atcagtgggc gaaggcttac aagctgatca
      421 taattttcgt tgatcgtttg gtcagcaaag cttgttttca ttgcctctac tgctttgtgc
      481 agttctttct ccgcttcgtt ttcgaacagt tcagggtcta cttctgattg tttttcaagc
      541 tccgcttttt tcgctaagtt cacgacacgt gtcaaagctt ccatcgatgg tttgaagtct
      601 tcatctgcaa gatgctgctt gaacaattgt gctgctgcaa atacttttgt cagatctttt
      661 tgttctgcgt tcaaaacagc ttcgataaca tcatgacgga tattcttcgt tgtcaataat
      721 tggcgcagac ggcctttgat aaagtcgatc acctgttgtt gtcccgctga caagccaatg
      781 ccaaaacgat ccgtatcttt actgataact tcactaatcg tttcttgcag aacagacaaa
      841 ggaaaggccc attttttcga ttcgatgatt cgaacgacac catatgcttg acggcgcaaa
      901 gcatatggat cgtttgagcc acttgggatc atcccaactg caaagaaaga aaagatacta
      961 tctaatttat cagcaatcgc taaaacagaa cctactgtag tctcaggtag atctccttca
     1021 ctggatatag gcatgtaatg ttcacggatt gctgttgcta cggctggttt ctcgccttta
     1081 agcagagcat atttttctcc cataatccct tgtagttcag ggaattctcc taccatgttg
     1141 gttactagat caaatttata gatttcagca gcgcgttgca gatccatcaa ttcttcctca
     1201 gatagtccta cttgttcgcc tatgatgcga gcgatgactt gtacccgttg cattttctca
     1261 tagatgctac cgattttttc atggaaagta acagttttta atttttcaac gtattggtcg
     1321 attgaagctt tcttgtcttc attataaaag aattccgcat cttctaaacg agcagtcaat
     1381 actttttgat tgcctcgtac cacattttcc aattttaccc gatcgccatt acgagcagaa
     1441 ataaaatgag gaagcaacat accttgatcg ttccgtactt cgaaataacg ttgatgttct
     1501 ttcatagaag tgactagtac ttcttcagga acagaaagat atttttcctc gaagtctcca
     1561 gaaaagacag ttggatattc gactagattg ttgacttctt ctagtaaatc ttcatctaga
     1621 tcaatgatcc agtgattttt ttctgctaat tcgtttgctt gtgtaacgat catttcttgt
     1681 cttttttgcg gtgaagcaat aacaaattgt tcatttaatt tttcttcgta ttcttttgca
     1741 tgcgcaaatg ttacatcttc gcctaagaaa cgatgacctc ttgaagctct gtctgttttg
     1801 acatccaaga tctcaaacgg gattatttcg tcatctaaca atgccacgat ccagtgaata
     1861 gggcggatat attcaaaatc gtattctgcc caatgcatcg ttactgggaa agtgaggctt
     1921 ttaatgacat ctgctaaacc tgtcaatact tcttttgctt ctttgccgtg agtgtatttc
     1981 gttacataga catattccac gccttttaat tctctaaaag tgatagcatc tgtcgtcaga
     2041 ccttgtccgc ggacaaatcc ttcagctgct ttcgtccagt ttccatctgc gtccaaagca
     2101 atttttttgg ctggtccttt gacttcttct tcgctgtccg cttgtcgttc tggaatattt
     2161 gttactttga ttgctaaacg gcgcggtgtg gaaaatgttt cgatcgtatc ataagtcaat
     2221 tggttttcat ctaaaaattt tgctgttttt tcttctaatt gtttcatgct tggtgtgact
     2281 acatgagcag gcatttcttc cagcccgatt tcaagtaata agtcttttgc catcttattc
     2341 agcctccttc acgttttctg gatttgctgt gtttttattc aataaaggga atcctaattt
     2401 ttcgcgttct gccacaaaga ttttggctac agcacgagcc atgttgcgga tacgggaaag
     2461 ataacctgct cgttctgtta cagatactgc accgcgagca tctaataaat tgaatgtatg
     2521 gctgcatttc aaaatataat catacgctgg atgaacaaga ttttcatcga tacagcgttt
     2581 agcttctttt tcaaaacggt caaaattgct tagtagtaag tcttggtcgc ttacttcaaa
     2641 agaatatttg gaatgttcat attctggttg tttgaagatc tcgccatatt tcacgccatc
     2701 agcccattct aaatcataga cactttcaac ttcttggata taagaagcaa gacgttctaa
     2761 tccataagtg atctcagaag taactggttt acaaggtagt cctccgactt gctggaagta
     2821 cgtgaattgt gtgatctcca tcccatcaag ccatacttcc cagcccacac cggcacaacc
     2881 catcgatggg ttctcccagt tgtcttcaac aaaacgaata tcgtgttcta atggatcaat
     2941 tcctaaaagg cgcaagcttt ctaaataaag ctcttggata ttttcaggtg aaggtttcat
     3001 tacgacttga aattgatgat gctgatacaa acggtttggg ttttctccat aacgcccgtc
     3061 agctggtcgg cgggatggct ctacataagc agcattccat ggttccggtc cgattgcccg
     3121 taagaatgta tatgggctca tcgtacctgc tcctttttct gtatcataag cttgcatcag
     3181 catgcagccg tttgatgacc aaaatttttg taatgttaaa atcatatctt ggacggtcaa
     3241 ttttttactc atctgttttg cccctttcaa tttttaagcg ttgctgtcaa taagaataca
     3301 ggaaagaaaa cgacaaaaag tccctatgtc tgattacaga catagggacg aataatttcg
     3361 cggttccacc ctacttccga ataaagattc ggcagcttca tttatcgtac tcgcaagtgc
     3421 ctcttcaaaa atcgtttggg tccggcttcc actctcccga actcgctaaa ccacgtgttt
     3481 tttactgatc ttggtcaatg tacgtattca ttttgcagtt ttcttgtttg tataatagat
     3541 cctctcagtg aacgatctat ttaatctacg gttaataata gacggaagtc cctgtatttg
     3601 tcaaatagtt ttgatttttt tgttgtatct ttcatttatt cttctttttc aggtatctct
     3661 ttttcaggca tatttttctc tggcgtcttt aatacgtgtg cccaagactt catttgatca
     3721 atgaactttt tgctttttag atgaatacct acatattcat catacaactg gtcaatgagg
     3781 tcacggattg cctgcttcgt ttcttctttc aagtggattc cactgatctt atcataagaa
     3841 atagccgaaa acattcgaac gaaataaaca gcgcgaggat ctgcatgata acggtgttcg
     3901 tccattggcc agtgtttctc gcatatcaca ccgccatatt tggaagaata gtcaaatttt
     3961 ccttttgttt ccccgcatac gacacagtgc gtccaaattg gcgcgattcc gaagcgttgc
     4021 agtatttgaa cttcaaagat attcgtgatg atttctgcat catctccttg gtccatccgc
     4081 tgcagagctt gttgcgtaaa ttggaataaa tgcgggtcat attgatgatc ttcgattgct
     4141 gcatctacta gatttagcaa atacgtacca taaccgctca aaaagatatc ttcttggatg
     4201 ttccgaaacg catggacgtc tttgacactg cttaaaaacg acaaaccttc acttcgaaaa
     4261 ttaccgatat agactgcttc ggtaaagggg agcaaagccg gagctaatgg ctgattttgc
     4321 cgatggattc ctttgatata aaacatcatt ttgccagaag attccgtaaa gattttgact
     4381 aatttatctt tttctttgta attacggcta gataaaatca agcctttcga ttctgcaagc
     4441 tggctcatat tctccctcct aaaaaataaa tctggaaaag cagattctgc cgatccagat
     4501 tatttatgtt acgatcttcc gctgtttttc tcaacgtacg gatttttatc cagtttattt
     4561 ctatacctaa cctataagaa gtccagaata tcagtactcg tcttttcggt agccgaagtc
     4621 ttgcagatag acttttttgt ctcgccagtc tttttggact ttgacccaaa gttctagaaa
     4681 tactttgtct cctaaaagat tttcgatatc ctttcttgct ttcgttccta cttgcttcag
     4741 catttttccg cctttgccga taatgattcc tttttggcta tctctttcga cgatgatcgt
     4801 ggcctggata tggattttat cattctcgtt tcgcttcatc gaatcaacaa cgactgcgac
     4861 agaatgaggc acttcgtctc gtgttaaaaa caagactttc tcacggatca attcagaaac
     4921 gatgaagtat tcaggatgat cagtcacttg atcatctgga aaatattgtg ggccctctgg
     4981 catttcactg actaatacat ccattaagcg ttcgacattg tttccttctg tagcagagat
     5041 cggaatcact tgtgcaaaag gcatctgcgt cgaataatct tcgatgattc ccagcaactc
     5101 gtctggatgg atcttatcga ttttattgat aatcagataa actggagaat gcacgttttt
     5161 tagacgttca ataataaaat cgtctccccg gccgcgtttt tgatcagcac tgatcataaa
     5221 taagactgca tccacttctt tcaacgcact gtaagctgct tctaccataa aatctcctag
     5281 acgatgcttt ggtttatgga ttcctggtgt atcgataaaa atcagctgag cttctggtgt
     5341 agtatatact ccttggattt tatttctagt tgtctgtgcc ttgtcactca taatggcaat
     5401 tttttgaccc acgattcggt tcaataatgt ggattttcct acattcgggc ggccgacgat
     5461 tgcgacaaac cctgatttat gttctggcat atacgttctc ctttttaatt aaacaacaaa
     5521 tgatatattt tagggacaaa taaaatcaac ccaatgatga cagcaaaaca agcggtcact
     5581 aaaactgctc cagctgccat atctttgatt tttttcccaa tcggatgaaa atgaaaatcc
     5641 gtaaacatgt ccactacatt ttcaaaaaca gtattgatga tctctactat ccaaacgaga
     5701 aaaacagcta aaagcagcca taaccactct atccgatcaa gctggaaaat cacgcccata
     5761 aataaagcaa ggataccgaa cgcaacatgt ttcctcatat tgcgttcttc ctcaaaaacg
     5821 gttttgactc cttggacagc aaattccacg gacatcagga aatgtttgtt tttctctacg
     5881 tttttatctt ttgagtccat aggcatccaa gatctctttc tgtaaaccaa acatttcttt
     5941 ctcatcttct ggtgtcatat ggtcataccc gttgatatgc aagaatccat gtaccgctaa
     6001 aaagccaagt tcccgatcat atgaatggcc gtaagcttct gcttgttcag cagcacgttc
     6061 aatagagatc atgatgtctc ctagtacacg aggcaatgga ttctctccat cttcaaaaat
     6121 cactgggatt tcgtcttcgc cttcttcttc cagagcaaaa ctgatgacgt ctgtcggtgc
     6181 atcttttcca cggtaatcac gattgattac ttggattgcc gcattgtcca tgaaagtaac
     6241 agacatttct gtttcctctg gcagctttaa ataagatgct gcaaattgca gcaagtcatc
     6301 gatttctttc attttctctt ctgagacttg gtttgtttca tccataaagg taatatccat
     6361 tatacatcgc ttctttctat tcttcttgtt cttcctttat cttctcaact tctgttttca
     6421 ttttaggata tttgatccga gaatggaagg tgctacttaa accgctgatc aatgattttt
     6481 ccacttttcg gatttctttc aaggtcagtc cactatcgtc taattgacca tcggaaatac
     6541 gctcttcgat aagattatgg acaaaacttt cgattttttc gctcgtcgga tgatccattg
     6601 cccgtactgc agcctcacaa ctgtcagcaa tattgacgat ccctgcttca cgactttgcg
     6661 gttttggtcc aggataacgg aattgctctt ctgttacttc cggattgcgt tctttcgctt
     6721 tgatgtagaa ataacgcatt agcgtggttc catgatgctg tttgcaaata tcgatcacca
     6781 tttgcggcat attataatct tccaatattt tggctccatc tgtcacatgt ccaaagataa
     6841 tatgtttact gtcttctggc aataagaaat tgtgcgggtt ttccgctcct gaaggcaagt
     6901 tctcaacgaa aaagctagca tgtttgatct tgccgatatc atgataatag caagctactc
     6961 gagttagcaa agaacgtccg ccgatttctg ctacagcgtt agcacttaga ttagctacca
     7021 tcatactatg atgataagta cctggtgctt cttccagcaa ctgtttcaat aaaggatgat
     7081 ttggattgct gagttcattt aatacaatta cactgtcatc attgaccatc aattcaatat
     7141 atgggtgtag accgatcgtc agcaagaatg agaagagtgc accagtgaac ccgcagacga
     7201 gcattaacca agtcgttgca tcaccaaagc tcattccttg atagacgatc aaaatgacat
     7261 ccatcatcac aggaaaaata atgacccaca tggcagcaga taatccttgt tctgagatcc
     7321 gtttgcgtct gacaatcgtt gccatgagcc cagagaaaac ataagacatc aagataatcg
     7381 ttagaaagtt cgtgccaatc gaatcataaa aaatgaaaag tgcggatact gcttggaaaa
     7441 taccagccat gatccctgct cttcgattca agaaaaagct taaaacaagt ggtgcaaaag
     7501 cagcgggata aaacagcggg atataagtag cttgttccgt ttggaataat tggaaaaatt
     7561 tcatcagcac tatgctgact gacatagctg tcacatagaa aagcacgtat tttgttcttt
     7621 ctcctgcatt ttggaattga agtgaattat aaatcagtac gccgatttga agcaaaacag
     7681 ctaaaaccat ggcaattagc ggaaaaatcg aagttgtttg attagtcagt cctagtaatt
     7741 ccaatttttt catagctgaa gcatcgattt gattaccttc acgaacaatg atttctcctt
     7801 ggaagatcat cactggttgt acagcttctc gtgcggattg tttcaattct tctgtctttt
     7861 tctcattcaa tacgtcattc actacgatac cttgatccac taagtagcga atcatttgtt
     7921 gttgtgctgg tgtgacattc aaatattgga tttgctcttc tgcttcttgt ttaaaagctt
     7981 ccaattcact ttcacgaact tgtttgctca tctgttcgtc aatgagcttc agactctcat
     8041 ccctcacagt atggatctca gtttcagtca tttcaaatat cgtcgaataa aaattgttcg
     8101 gtagtttttg ataaaacgca acattttctg cattcacatt ttcaaaattt ttcttcagcg
     8161 cggcgatccg ctcttctgta gtaggctttg ggatattttc atcttccttt gccttatcaa
     8221 tcttttcgtc ataagatttg ttgatggcat cattcgtctt atcgatcaat tcaaatagtt
     8281 gcttgatccg attgttctgg tcatctgcaa gatctttttg gtaagtgtat tctggagtga
     8341 ctgcttctgc tgccagtttt cttttttgtt cggtttcttc cgtgttctcg atcgttttgt
     8401 tcgcacggat actttcttct gctacttggc cttctctgaa atcattggcc ttttgtttga
     8461 cactactaaa tgtaacgccg cagataatga taaaaaacaa gaacagcagg aaaggcaatg
     8521 ctgcctttcc aagtttttct tgtagtttct gcagtgtttt attcaacatg tgcttatcct
     8581 cctaagcaaa gcttattctt gatggagatc tgccttttca taagctttga tgatttcggc
     8641 aactacaggg tgccgaacaa cgtcacttgc ttcaaaatta acaaaatcga ttttcttgat
     8701 ttgctttaat gttcgttctg catgaacaag accacttgtt gtccctcgag gcagatcaat
     8761 ctgactcgta tctccattga caatcatttt tgattgataa ccaagacgtg tcaggaacat
     8821 tttcatctga gcaactgtcg tgttctgtgc ttcatccaaa ataacgaatg catcatccaa
     8881 tgtccgcccc cgcatgtaag ctaaaggagc aatttcgatc acgcctcgtt ccatcaatcg
     8941 gttcgtatga tctaatccaa aaatttggta taaagcatca taaacaggac gcagataagg
     9001 atctactttt tcttttagat ccccgggaag aaagcctaga ttttcccctg cttccacagc
     9061 aggccgcgtc aaaatgattt tttgcacttc accttttttc aaggcagcga ttgctaatac
     9121 tactgcaaga aaagtttttc ctgtcccggc tggtcctacg ccaaatacga tatcatgttt
     9181 ggctaccgat tcaacatatt tcttttggcc gctgtttttt acccgtatag gttttccatt
     9241 acggtctttg acgatctctt cctcatacat ctcaacaaaa taatctaatg tgcctttttg
     9301 ccccattttc agtgccgtca cgacatctgg tgtacttaca tggattcctc gtttgatcaa
     9361 ttcctgcaat gtccgaatga ctgaggcagc caaggaaatc tgggattgtt caccaatgat
     9421 ctgaatgatt tctccacgtg tatggatggt tgtttctgtt gtttcttcaa tcagttttat
     9481 atgcttgtcg tgtgcaccta aaagcatgct gacatcatca gcagccgtca atctaatctc
     9541 taaagaacta gactcttccg tcaaaaatag acatcccctt tttttcgatt cattctttcc
     9601 tattttacca tactcagaag aaactatata gcaaataaga aaaggttcta aaagagttgc
     9661 ttaaccataa gtaccaagaa acgttaaaaa atagctgctt ccattctcta aaggtctcgt
     9721 ttgaataaaa aaagctgatc atgtaagcta tgcataagat agaggacaag tattccgtct
     9781 aacttagcaa gtattctctg ggtatttttc ttgtttcaaa aaaagaaaac aaaatatttg
     9841 aaatattctt gcatttcaaa aaaaacaggt gtattataac gtcaacaaat taatatacag
     9901 caaagcgtcg aggaaaacag taggctattc agcgatttat tcccagtgag tcatgtaagt
     9961 gagaaatgac aataaatcga atagacgaac atcatttccg agtcaatgtc gaaaacgtag
    10021 atcattgtgg ctgcctccat tatcaggcta gcggataact ttatcgaaaa gcgtccgtga
    10081 atgaagaagc ttttttaagc gaaaaaaggt ggtaccgttc tagaatctag agcccttttc
    10141 ccaagtggaa aagggctttt tttatatttt aaggtggtgg ttttcatgga atctgatatt
    10201 agtggtattt ctagtttctg caacgtttat tttgttactc aaaatgataa ttattggagg
    10261 aaaaatttat ggaagaaaca aaaacttatt catggaagca gttattgatg gtcagttcac
    10321 ttatcttcgg gatgttcttc gggtcaggta atctgatctt tcctgtccac ttaggtcaaa
    10381 tggctggatc aaactggatc agcgctggtg taggatttgc catctcaggc gctcttttcc
    10441 cattattagc tatcttagct gtagtagtga caaaaagtga tggtctgtat gacttagcta
    10501 aacctgtcgg aaaatggtat gctgccttgt tcttgatcct tgttcattta acgatcggac
    10561 cattctttgg tacaccgcgg acagcagcaa ctgcttttga aatggctgct aaaccctttt
    10621 tgcctgaaaa atatgattca ttaggtatgt ttttattctc tgctcttttc tttttacttg
    10681 cttatttcct tactgtacat ccaacacgtt tggtcaaata tgtcggaaag tatttaaact
    10741 ccttgtttct tgcattatta gctatcattt ttgtcgtttc tttattccaa cccatgggga
    10801 atctaaacca atctgctgca gattcttatc agacgaacgc tttgacaaat ggcgttttag
    10861 aaggatacaa cacggttgat gcagtagctt tacttgcttt aagtgtcact tttgtccatg
    10921 cagttcgtga tcttggtttt aaagataaaa aagtcacaga attaacagca aaagctggta
    10981 gcctgagcgt tgcgttagaa gtcatgattt attttggtct ggtccttctc ggtgcgatga
    11041 gtttgaataa gatgtcttta tcagaaaatg gcggaactgc actttctcag atcactaatt
    11101 tttatttagg taacttcggt gctagcttct taggggtaat ggtcacatta ggtgtcttca
    11161 ctactgctat gggactcgtt gtttcttttg cacaagattt ccataaactg ttccctaaag
    11221 tcagttatat gacatggctg cggttaacga cttttgtctc ttttgtcgtt gcgaatgccg
    11281 gattggataa tatcatccaa tggtctttac cagtattgat gcttttgtat ccattgtcac
    11341 ttgcgttaat cttactttcc ttaacagcaa aattcttcca aaagagtcca tttgtctatc
    11401 aagtaacgat gttcttttct gccgttccag ccgtattgga tatgttggca aattcgcctg
    11461 cattagtgag tcagcaaact gtcgtagcaa gcttactagg attttatcat cattatgttc
    11521 cttttgctgc attaggtctt ggctgggtcg tcccaacgtt gcttggttac gctggcagct
    11581 tgctctttta ttatgtatat cgcgtatcag gatataaaca agaagcaagt gagttaccag
    11641 aagaataacc aaatcaaaca caatttaaca taagagatat taaacaggaa aaaaaacagc
    11701 tagaagaaag aataagattc tttcttctag ctgtttttta gttctttttt tacgaac
//
LOCUS       DACECR010000090        11731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.090, whole genome shotgun sequence.
ACCESSION   DACECR010000090 DACECR010000000
VERSION     DACECR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.090"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(284..2068)
                     /locus_tag="IWO17_002230"
     CDS             complement(284..2068)
                     /locus_tag="IWO17_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0291089.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(2068..3819)
                     /locus_tag="IWO17_002231"
     CDS             complement(2068..3819)
                     /locus_tag="IWO17_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0291090.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(3961..4185)
                     /locus_tag="IWO17_002232"
     CDS             complement(3961..4185)
                     /locus_tag="IWO17_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ0291091.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            4436..6022
                     /locus_tag="IWO17_002233"
     CDS             4436..6022
                     /locus_tag="IWO17_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ0291092.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(6088..7863)
                     /locus_tag="IWO17_002234"
     CDS             complement(6088..7863)
                     /locus_tag="IWO17_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ0291093.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8074..8976)
                     /locus_tag="IWO17_002235"
     CDS             complement(8074..8976)
                     /locus_tag="IWO17_002235"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ0291094.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            9255..11600
                     /locus_tag="IWO17_002236"
     CDS             9255..11600
                     /locus_tag="IWO17_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0291095.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT         3557 a         2403 c         2186 g         3585 t
ORIGIN      
        1 acccaaaaag ccgtccatgg aaccatggga gactcatcag gtttagcttg catcaggcaa
       61 tcactatcca agttgtatcg ttttctttat attatcgaaa gcaaacgctt ttgtcaacgc
      121 tttcttagtg gttattagcc gcttttgact agctgtcgtc gtctttttta aatcgggtga
      181 ctacgccact aacgctcaca gatgaagaat ctgcgaagct ctgttttatt ttcactctag
      241 cgcttagggc aaaatgctac tatgctactg tcacaacctc tttttacaca ccactgcttt
      301 gcagtttgta catatctgca tacaaaccat tttgcgctag aagtgtttca tgattcccac
      361 gctctactat atgacctttg tctaaaacta aaatcaattc tgcatcccga atcgtggaca
      421 gccgatgggc aatcgctatc gttgtccgtc cttgtcgcat ttttgccaat ccttcttgga
      481 tcaatccttc ggtttcagta tcgatatttg ctgttgcttc atccaatact agtattttag
      541 gatctgtcac gattgttcgc gcaaaagtga tcagctgtcg ttgtccacta gaatagctgg
      601 caccgtgttc gatcactttt gagtgatatc cttttggtaa ttcatcgatg aacttgtttg
      661 cttgaacaaa ctcagctgca tggcgaatct gttcgtctgt gatagctgga ttcatcatcc
      721 ggatatttcc tgcaatatct ccataaaaca agaaggcatc ttgcaagacc aatcccattt
      781 tcttacgtaa ctcttccatt ggatattctc gaatgtcatg atcatcaatc aagatttggc
      841 cttcataaaa ttcgtaaaac cgcatcaata cgttgatgat cgagcttttt ccgcttccgg
      901 tgtgccccac taatgcaacg gtctcacccg gattagcgac aaaagagata ttgttcaata
      961 cattgtgctc tccatcataa gaaaagctta cattgcgaaa ctcgatctta cctgaagtaa
     1021 tcgcttgatc tgctccagga ttttgttgcg gtgcatattc ttctgtatcc atgattttca
     1081 aaatacgact accggcaacc atcccatctg taaaggtgct caaaaagtcc atcatttgag
     1141 acattggatt aaaaaattcc tggacatagg tcgtaaatgc atagatcagt ccaacttcta
     1201 cgggagaatg caacgcatcg atcccaaaca aagtcaacgc caacgcaatc gccaatgcat
     1261 agagaaaatt gatgattgga cttaacagaa tcgaattcgt tttgatcatc gcatagcgcg
     1321 tgtctaaata ctcgttgttc acttgttcaa attctttagc taaacgagct tcttggcgaa
     1381 actgctggat aatacgcatg ccggaaatcg attcattcaa tttcgtgttc agctgactta
     1441 atttttcccg catatttcgg taaatacgag aactgaattt ctggtaatac cagatgacga
     1501 ttcctaaaat cggcaagaac accaaagtcc acaaggcaat ttctccattg atttgaaaca
     1561 tggcaacgaa agaagaaacg actgcaaaga tcccagtcaa taccatcaaa aagacatacc
     1621 aaaattcaaa tagcgtttct gtgtcattcg tcactcgaga aacggtcgat cctgccggtg
     1681 tctggtcaaa ataacgcata cctagtgtat gcagcttttc aaataatctt acccgaacat
     1741 actgataggt cttcaatgaa gccatggaat acaaatacca ttggaaaaac caaatgatac
     1801 ttttgactat gactccaaac aagtacacac cagcaaaaaa caaaataact tgtgtcgtag
     1861 ctgactgttt agccagatga tcatccataa aagtttggat gatccttggc aataggacat
     1921 tgatcacaga cagggcaaac gcaaataaga tagcaacgat gaacgttctt ctaaaaggtt
     1981 tggcgaactt catcagtcgt ttgatgatct tcttctgttc tttaagtgga atcgattttg
     2041 tccattccga ttgataattt tcatccatca ggcctcgcct ccttctatct tcgcttctaa
     2101 ttgctgtttt tcccacattc gttcatacca gccacctaat gcaagcagct cgtggtgggt
     2161 tcctcgttca atgattttcc cttgatcgac aacaatgatt tcttttgcat gcatcacgct
     2221 gcttaaacgg tgagctgcaa tgatggttgt tttctctttt cttatttctt ttaaattatg
     2281 caagatagct tcttctgttt tggcatctac tgcagaaagt gcatcatcta agatcagcaa
     2341 ttcaggattt ttgatcaatg ctcgagcaat cgaaatcctc tgtttctgtc cgccagataa
     2401 agagactcca cgttctccaa ctaaggtatc ataacctttt ggcaattcct tgatttcatt
     2461 gttgataaat gctaaagaag ccgcatgctc tacttcttct tctgaaagtt ttggattaga
     2521 aaaacggata ttatctcgaa tggtcatcga aaacaggaag tgatcctgag gcacataacc
     2581 gattgatcct aataaagcat tcaatgaata ttctttgatc gaacgtccgc caaattggat
     2641 tttcccttga taatcatcgt attcacgcat cagtaatttg atgattgtcg tttttcccgc
     2701 accagttttc ccaacaatac ctaatgtttc tccttcttgg atcgcaaatt tgatttgttc
     2761 taatgctggt gattcttctt tcggatagtt aaaagaggaa atagctacat tcagctcgcc
     2821 ttttgccggg gtctgcactg cacccagttg ttccacgata tgcgtctttt catgcaaaag
     2881 ttcattgacg cgatcataag aagcatttcc tctttccaat acgttgaaca atcgaccaat
     2941 cgcaaacatc ggccaaacaa gcataccgat atagctaata aatgagacca tctgcccgat
     3001 tgagatcgtg ccttccaaga catactttcc acctaaaata attgttgcaa cataagacag
     3061 accgataatc aaggtaatga atggatcaaa taaggagtct aaaaaattga ctcgtttgtt
     3121 tttaataatt gcatcttcaa tcttttcttc aaaatccgcg agatcttctt tttcttgtcc
     3181 aaatgtttta atgactttga tcccagtgat actttcttgt gtcttatcat tgatactaga
     3241 aaaagcttct tgcgaatcac gaaaagcatc gtggagtttt gaccccagca ctcgtgaagt
     3301 aacggctagc aaaggcagag gaatcagcgc aatcagtgtt aagcgccaat caataaacaa
     3361 aatcatcgca atgatcgttg ttccgcccgt aatcactgaa tctgcaaagg tcaagattcc
     3421 agctcctgct acattctgga ttgcattcaa atcattcgtt gcatgagcca tcaaatctcc
     3481 agtccgatat ttttggtaaa agagattgtc catttctgta aaatgtttga atagctgctg
     3541 ccgaagttct ctctccaacc gagctgcact tccccaaata ttcgtccgcc agatataacg
     3601 aaaaagatat tgtaagaaag ctgcaaggat caataataag atccaccagg aaatgatctt
     3661 tattgataaa ttcttatcgg cgatctcatc aatgatgatc ccaatcactt ttggaggaac
     3721 taactgtacc aaggccactg cgaataggga gaaaatccca attaaataat gtttcttttc
     3781 ctgacgaaaa aaccaaccta attttctaaa aatcgacatt ttactcctcc tttgtcttta
     3841 tcaagagcat aggattgtct gtgcttgtct tttttgctga atagtctgaa tagtctaaca
     3901 aattacgaaa taaaaaacgg actgaaaaaa tcagcccgca aaaagtcttt gggtcagaac
     3961 ctattttttc ccttggttct tcatttgctg catcatctgg cgaattttct tctcagatgg
     4021 tttttgcccc attgacatca tcattgatcg caacatgtcc tcattaacgg gaggattttt
     4081 ttctaaatag tctttcatat atttacgcgc taggaaaaag ccacctactg cgccgattag
     4141 caaagcaata atagcaataa gtacgacaat acctgttgat accatataaa tctctccttt
     4201 ctatttccga attgctctca atgatttatt ttactaaagt ttattgaaaa aagaaaggcg
     4261 tattctttag tttcatgcaa aaaatagtgg tttttttaga gaagaacaaa atttctttca
     4321 ttaaccagca agacacacca cgaaaaaaaa ttaggcatac cgaatctttt tctttaattt
     4381 tttctttctg aggtgtagaa tgtaatcgta gataaatagg aaaggtggtt ttttcttgaa
     4441 taatcaatcc aacaaacaat ttcataagca tcgacttatt gaacatacaa atggtttatc
     4501 tttagctgaa gtaaatggaa cagtggatgt accaaaggga aaaagttttt ttaaaacact
     4561 tcttgcttac tctggaccag gtgcattagt agcagttggt tatatggatc caggtaactg
     4621 gtccacttcg atcactggtg gacaaaattt ccaatattta ttgatgtcag ttattttgat
     4681 gtctagtcta attgctatgc ttttacagta tatggcagca aaattaggaa tcgtgaccca
     4741 aatggatttg gcccaagcaa tccgagcacg gacaagtaag acattgggtg tcattttatg
     4801 gatcttgact gaattagcca ttatggcaac agacatcgca gaagtcattg gtgcagcaat
     4861 cgccctctac ttactttttg atattccatt ggtgattgcc gtctttatta ctgtattaga
     4921 tgttttcgtt ctcttactac tgacaaaagt cgggtttcgt aagatcgaag cactggttgt
     4981 ctgcttaatt ttcgtcattt tattcgtttt tgtctatcaa gttggcttgt ctaacccaga
     5041 ttggcacagt ttatttgctg gctttatccc taacagtcat acattctcag actcaccggt
     5101 catcggcggc gaaactccgc tgactggagc tttagggatt attggtgcaa cagtcatgcc
     5161 gcataactta tatcttcatt cagcagtttc acaaactcgg aagatcgatc accaaaacga
     5221 agaagatgta gcacaagctg tacgtttttc tacttgggat tctaatatcc agttgtcaat
     5281 ggcttttcta gtcaatgcac tgctattgat catgggtgtc gctgtcttca aaaccggttc
     5341 tgtccaagat ccttcctttt ttggtctgta tgaagcttta tctgatccat cagctatgag
     5401 caatggtatt ttgattgctg tagcaaaatc tggtatcttg tccactttat ttgctgtagc
     5461 acttcttgct tctggccaga actctactat tactggtacg ttgaccggtc aagtcatcat
     5521 ggaaggattc atccatatgc gtatcccgat ttggctaaga agactggtca cacgattgct
     5581 ttctgtgatt cctgtattga tttgtgtttt gtttacaagt acaaaaggaa cgatcgaaga
     5641 acatgccgct ttgaataact taatgaatga atcccaggtg ttcttggctt ttgcgttgcc
     5701 attttcgatg attcctttac tgatgatgac caacagccaa gcggagatgg gtagccgatt
     5761 taaaaattca ttcatcgtaa aacttcttgg ctggttctct gtgattagtc taacttactt
     5821 gaacttacga ggattaccag gacagatcga aagtttcttc ggcgagcaag ccacttccgc
     5881 tcaactagca ttggctgatc acatcgctta tgtaatcatt gttcttgtcc ttctcctgtt
     5941 ggcatggacg attggtgatt tatataaagg aaacaaaaaa tatgaacagc ttttgcagga
     6001 aacggtagaa gaaatcgaat aaaaactgat ttttccacac aaagaccctt gatagttagg
     6061 ttaaaaatct agctaccaag ggtcttttta tttagaagat aaagcttttt tcagatttaa
     6121 tttactgaaa actagcatgc ttgttactac gatgaattgc gttaatggca cgcctgcacc
     6181 agtgtagaca cttaaaaaat agtccttttt ttctggcgca taagaacaaa accatgagac
     6241 accgaaaaga aaaaccaatt gtgtcagtat taatccggtg accacaatca aagaggaact
     6301 ataataggca cgatcagctt ttgcaaatac ggttcctagt atcataagta atatccctgc
     6361 tattgaagca atcagcacgg gaatagccag aacttggtaa atttttatga tgacattcgc
     6421 gacttttgct gattttgtta attggaaatt aggtgttctc ggttcattac tatcgatcca
     6481 attctccatt agcggaaggt ttaataattc ttgagcctgt ttgacttgtt cttgcgttcc
     6541 gaaactataa tttcctcctt gatggaaatc tttatatcgc catgcataag cgtatcctga
     6601 ttgaaggaaa tcgaatacga ttggcagatc actcatcgtc tttccatctc ctgatcccat
     6661 cagatagaac tcttttttct ttttgatggt tcccttgtga taggcttctt ctaattcttg
     6721 gttgactttt ttccaaaaat cattggtttc ttttgcattg ttttggtaat aaccagattc
     6781 gcttaccaca tctcgcaatg cccaaaagat gatatctccg ggtaattctt ttacttttcc
     6841 accttgagtc cacgcgtgag tcgtatataa ttcgtcaata cgggtagaaa cagtatggaa
     6901 tgtaggagat gcggctaatg ctttatttag tgcagcatgt gagacccata tctcactgtt
     6961 ttctttattc aagttcgttt catcatctat tcggatcagt tgtttcatca aacgtccaaa
     7021 ttctccactg gaacggtcat tgatggttgc aataccgtat gttgtctcat ttttatattc
     7081 gatccactga tagacgatgg aaaagccaac tataggaagt aaaaacaatg aaagtttcag
     7141 ccattcccgt ttcgagagaa gaaagagtcg tttttttaac ttctttactt ctttgaaaga
     7201 aaagtcttgc cctagcaaga tctggacgaa actgataccc aaagctacta cagtaaaagg
     7261 aagaagccag atagaatctt cgcgaatgaa ccaaaagaaa ggaaaagcga cagaaagccc
     7321 taaaaaccat ggcagcaatt ttctgctaga accgtatttg cggtaataaa tagctaataa
     7381 acaagcgata atgatctcaa cagccggaat cacgagggca ttacgatatg ttcgagtgga
     7441 atattcatgt gtaaaactaa tcggagaaaa caaaaaataa aaatagatac ttaaaagaag
     7501 atacttgttc ttcacaatcg gtttaaacgc gagtgcaacc aatatactgg caaaaatact
     7561 gaacatgccg aacaaaactg cataagaaag atgcagttta tgtgcaagta ctaaaaataa
     7621 gctgtaggag attcctttgg aaagtgttgt agtcgtatac tcccctagcc atttaccatc
     7681 caaaatattt tgagcatagt gaatcagcag ctgatcatca taggtcgccg acatatctgt
     7741 tccatagatt gctttactat ataaatacaa ccgaataatt gtaaataata cggctatgcc
     7801 aagaataaag tatgtatgtt tcttttttag ttggtcgatc aaggggttta aaatgttcag
     7861 catcttttcg tcctttcaat cacgttttgc tacttgtttg tgaaactttt cgttttttac
     7921 ttttctatca gtataccctg tttgtctgaa tttaaaaaga gggggcaaaa ggagcatggc
     7981 gaaaacgaat gcaaaaagca ctcctttgtt tcttaaacaa aaaaggacaa ggcagactgc
     8041 ccaatccttt tttgttgctg tcaggtagaa aacttaaagt gtgacttctt ctgctaataa
     8101 ctcagggaac acttcgggat cgaatgcttg cgctttgaac atggaaattt cttttccata
     8161 tggtgcgtaa gctttctttt tatctttacc gacatatggg gtttctaata ttttcgaaat
     8221 gttgtcaaac attggatcat ggacgatttt gttcaatgtg tcaaatccaa ttgttccaaa
     8281 accgatattg gcatgccgat ctttatgaga accttgtgga tttttagaat cattgatatg
     8341 cagtacttta agccgatcta atccgatgac cttatcaaac tcttccatta cacctgaaaa
     8401 atcttctttt atattatatc cggcatcatt cgtatgacat gtatcaaaag taacggataa
     8461 tttttcattc aattttactc catcgatgat ttgcgctaat tcctcaaatg ttcgaccgat
     8521 ttccgtacct ttccctgcca tcgtctccaa agcgatctgt gccgtctgtt cttttgtcaa
     8581 aacttcattc aacccctcaa taattctagc aatcccagct tctgcccctg ctcctacatg
     8641 agcgcctggg tgaagggtga tttgagtagc acctaaagct tccgcacgga caatctcttc
     8701 tctcagaaat tgtacagcaa atggaaacat ttctggtttg atcgtatttc ccaaattaat
     8761 gatataaggc gcatgaacga cgatatttgt tagtccgttt tctgccataa atgcttttcc
     8821 ggcatcaata ttcatctctt cgatagcttt tcttctagta ttttgaggag cgcctgtata
     8881 gatcataaag gtcgaagatc cgtaactggc tgcttcttct gccgcgccta acagcatttt
     8941 ctttccgctc atgctgacat gtgagcctaa taacatggat ggattcctcc ttgaaaggca
     9001 attgtttcga acaattcctg tatattgata agatgatgta caaagaaaat ggtttgtttt
     9061 tctttgccct gttctccata gtaactgaaa ctttccctaa agaaaagctt aagaactctt
     9121 tcataaaaaa gatcatttcc tcacgtttta tctcattcga aaaagaaatt cctgtcaaaa
     9181 acagccttgt ctgttttttt gtcctattat ttgctataat tagcgaaata aaaccattag
     9241 ttgaggtgac ccttttgcca cgcaaaaata ctcgaaaaaa acagaataga agaaaaaaag
     9301 aaaaatggtt tgttcctaag attattttta gagtgtttca atcattgact gtttttatta
     9361 ctgttcttct tttgttgttc gcagctctag gtatcggaat aggtgctggc tactttgcct
     9421 atcttgtcga agacacacaa ttgccgacta aaaaagaact gcagacagaa ctaggaaaca
     9481 ttacggaaac gtcaaagatt gtttatgcag ataatacaga gatatcaaaa atccagacag
     9541 atctgatgcg gacgactatt tcatctgata agatttcgcc tttattgaaa accgcgatta
     9601 tcagtacaga agatgaatac ttcgataaac atcaaggtta tgtccctaaa gctgttttac
     9661 gagcactagt ttcagaagcg actggcatcg gctcgtctgg aggatccact ttgacacagc
     9721 aattagtcaa acaacagatc ctaaccgatg aaacaacatt caaacgtaaa gcaaacgaga
     9781 tcgttctttc tgcacaagta gaaaaacact tttcaaaaaa tgagatcatt actacgtatt
     9841 tgaatgtctc accatttggt cggaataata aggggcaaaa tattgctgga gtccaggaag
     9901 ctgctagagg aatctttggt gtagatgcaa aggatgtgac tctgccacaa gctgcttata
     9961 tcgctggttt gcctcaaagc cctatcacgt atagtccata cacgaataca ggcgcgctaa
    10021 aagaagatct ttctgccggt cttgccagaa aagattttgt tttattcagt atgtaccgtg
    10081 aaggacaaat tacgaaagaa caatacgaag aagcgaaagc ctatgatctt acaaaagact
    10141 ttctcccaca acaaatcgcg gaacagactg acagagaatt cttgtattat accgtgatga
    10201 acgaagcaac aaggattatt gcacaacaat tggcagaaaa agataatgct gatatgtcag
    10261 attcatcggt cagtgaagct tattatcaaa aagcacagca aacgatccaa aacaaaggat
    10321 atacgatcca ttcgacgatc gataaagata tctatgctgc attacaaacc ggcgtagaaa
    10381 attatggcta catgttagat gatggtgctg gtagccaagt ggagactgga aatgtattga
    10441 tggataacag aaccggacgt atctatggct tcgtgggtgg tcggaattac agccagaatc
    10501 aaaataacca cgcctttgat acagaacgtc aagctggttc atccatcaaa ccggttcttg
    10561 tttacggtcc tgcaatcgat atgggacttg tcggatcaga atcccgtgta tcagattatg
    10621 caacgacttg gcaagaagga aaaaatgctg gtgagaaaat cgtcaatgcg accaatgaag
    10681 ggtcgaacac cttccaaacc gtccgtgagt cactggaatg gtccaacaat atcccagctt
    10741 atcacttata tcaagatgtg ttgaattccg gtggatcgaa acaatacgct tacgagcact
    10801 acttggcgaa gatgaactac ccagcaaatg ataattgggg cgttgaatcg gctcctttag
    10861 ggacagtaga tgtaacgacg cttcaacaga caaatggatt ccaggcctta gcaaatggtg
    10921 gagtctacga agaaggctat atcattgatt ccatcacaga taatgcaggt aatgtgattt
    10981 ataaacatga aagtaattct gtccgtattt attccgaagc aacggcatcg atcatgaatg
    11041 acatgatgcg tagtgtgatc aatgaaaaaa tcactacacc attcaaagat gtcatctcaa
    11101 gcttaaacgg aaatctagga aatgcagatt gggtcggaaa aactggttct accaatgaat
    11161 accgagattc atggctgatc gtctcaactc catctatcac tatcagtagt tgggcaggac
    11221 acgatgacaa tactggaatg gacagccaag caagaatacg ttcggctaat tatcttgcca
    11281 atctaatcaa tcaagcttac caagcaaacc ctgagatttt cggcactgat gagaaatttg
    11341 acttatcatc cgatgtgatg aagaaaaaag tatctgggtt cacaggacaa acagctggaa
    11401 aagttactgt caataaaaaa tccatccaaa cgccaggaaa aacagtggag tcactttggg
    11461 caaaaaatgg tccgaagaaa agcgccttta aattcggcgt aggcggtact gatgaaaact
    11521 atacagatta ctggaacacc gcttcagctt atgctagagc gaatccagag accaaagaat
    11581 cagacgataa agatgattga tctaatagat taatcagaat taaagggagc tgcgacaaaa
    11641 atcttgtcac agctcccttt ttccgaataa atggtgttca aaagcttaca tctacgatat
    11701 taagacaaat ttatgtaaaa atgtgaaaaa c
//
LOCUS       DACECR010000091        11693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.091, whole genome shotgun sequence.
ACCESSION   DACECR010000091 DACECR010000000
VERSION     DACECR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.091"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..401)
                     /locus_tag="IWO17_002237"
     CDS             complement(<1..401)
                     /locus_tag="IWO17_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291096.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLN"
     gene            complement(394..795)
                     /locus_tag="IWO17_002238"
     CDS             complement(394..795)
                     /locus_tag="IWO17_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291097.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            complement(785..1138)
                     /locus_tag="IWO17_002239"
     CDS             complement(785..1138)
                     /locus_tag="IWO17_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0291098.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            complement(1128..1439)
                     /locus_tag="IWO17_002240"
     CDS             complement(1128..1439)
                     /locus_tag="IWO17_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291099.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            complement(1436..2263)
                     /locus_tag="IWO17_002241"
     CDS             complement(1436..2263)
                     /locus_tag="IWO17_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291100.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLSFVNKKKCSYAYVKAIKAVVR"
     gene            complement(2273..3433)
                     /locus_tag="IWO17_002242"
     CDS             complement(2273..3433)
                     /locus_tag="IWO17_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0291101.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(3433..4119)
                     /locus_tag="IWO17_002243"
     CDS             complement(3433..4119)
                     /locus_tag="IWO17_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ0291102.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            complement(4082..5260)
                     /locus_tag="IWO17_002244"
     CDS             complement(4082..5260)
                     /locus_tag="IWO17_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0291103.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            complement(5280..6974)
                     /locus_tag="IWO17_002245"
     CDS             complement(5280..6974)
                     /locus_tag="IWO17_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0291104.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            complement(6952..7266)
                     /locus_tag="IWO17_002246"
     CDS             complement(6952..7266)
                     /locus_tag="IWO17_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ0291105.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            complement(7369..7650)
                     /locus_tag="IWO17_002247"
     CDS             complement(7369..7650)
                     /locus_tag="IWO17_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291106.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            complement(7655..7999)
                     /locus_tag="IWO17_002248"
     CDS             complement(7655..7999)
                     /locus_tag="IWO17_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0291107.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(9186..9653)
                     /locus_tag="IWO17_002249"
     CDS             complement(9186..9653)
                     /locus_tag="IWO17_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAQ0291108.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPSSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKLESNKIIIQFASSLELVAFK"
     gene            complement(10226..10513)
                     /locus_tag="IWO17_002250"
     CDS             complement(10226..10513)
                     /locus_tag="IWO17_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291109.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKEPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAVIRIENTMDCDKELAKSKANIAVVRLVDMKAI"
     gene            complement(10513..10707)
                     /locus_tag="IWO17_002251"
     CDS             complement(10513..10707)
                     /locus_tag="IWO17_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291110.1"
                     /translation="MKAYTIKFYGNKTFVYILSGLRLDFRGYSKDDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEA"
     gene            complement(10710..11096)
                     /locus_tag="IWO17_002252"
     CDS             complement(10710..11096)
                     /locus_tag="IWO17_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291111.1"
                     /translation="MKSLQQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLES
                     EDLRQIEKSIQLNDNQQIVFDFLKNEFKRNDLQYTLWSFTEDVYERLEIGVSSLPYVK
                     AWGKLKEKQQFEVLTAFAQWGLEQEE"
     gene            complement(11093..11632)
                     /locus_tag="IWO17_002253"
     CDS             complement(11093..11632)
                     /locus_tag="IWO17_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0291112.1"
                     /translation="MNKQELIDKLASLVGSIEDFKGISTFHDGKYAGLEHALELIKQL
                     DEPQKPFVPKFVADYIEEYRGGGATLYEMMDSSEDEVYTWLFGNPRIKTENERQLLFA
                     QAYAVGYEVEKEPLYEVIIGNLFLVKKFNNRNDSCFVNKFELCWWEKSAYQLTEPEIK
                     AIDERYWPFAVPVEEVAEG"
BASE COUNT         3349 a         2432 c         1727 g         4185 t
ORIGIN      
        1 ttatttaatt tatcgaattg acgaatcaga agttctgtaa ttggatcaag tgcaagacct
       61 aatcctcttc tcataaattc gtccggctga tttcttttag aagtacctat ccccaaatca
      121 ggatatttta aatactcaaa ttttcttgta ggtctaatga tgaaacccaa attaatgtat
      181 tgagtcttaa gtggacgact attttttgcg tgttggtgcc ctcttcttaa atctgcttca
      241 gaaacaggaa ttttttctgt aatcctatcc actgcaatag ccgaaccttt tgatttcaat
      301 gcttcgttaa tcagtcgttc gctctcgctt gaatagcgtt ccatccgcac aagaagttca
      361 tcatgtccat ttatttttag ctcccaacta tttttagcca tgacaatcac tcttcaataa
      421 tcgtctaaac gtaaatacca attgatcgat atagcgatct tggttctcta gttttaaatg
      481 attgggatcc attctctgaa aacgaatcga acgattttga atcaatgaaa taatatctag
      541 tgaatctcct gttaaatctt ctctattttc tgaatagaaa gttagatata gattttgacc
      601 cacgctatat tttggctcag tgatcatttc tatttctcct gtttcgagaa tgaagtaatt
      661 aaaatcatca ggtagctcat cctcgcctac ggagtcttga aagagtttga ggccaaaatg
      721 ctcttctaag gaagttttga tagcagaaat ttgcttattt aaacgttctt tttctttaga
      781 attatcaatc accatattca cccacacttt caagataaaa atagatataa aaattatcgt
      841 aatcggcata gataacgttg taacgcatac tatcgattac gataaaatat tgatctttat
      901 taaatttctt ggcgattgga tgaaatggag tctttacttt cttagttaat ttcgatccca
      961 tcgcatccat agctgttata tcactatctc tcatggaaag atttctaaat tttaaagaag
     1021 tgatttctgt atcttctaca ccaatctttt ttcctagttc atttcttttg gtagtttgcg
     1081 tcaaaatctt taaccaacca tcgttgaatg tttcttcgag tctacgatta ttcgccattc
     1141 acatcacctg caatatattc ttgtagcgca taatgttgaa tgaaacctaa taactcacta
     1201 gcgaaatttt gttcaaactc atctaaagca cgattccagt cgtatctaca tctttcgatt
     1261 agcaatccgt attctaagct ttcaggagaa aaagaaagtg ttgtactcac tttactttga
     1321 agataaacag catttttagc tatcatcttt ttaattgact catcttcttc gttccaggta
     1381 acgtaaatat tatccttcac agctattagc aattcttcag tcacttgttc aggcgtcatc
     1441 taaccaccgc cttaattgct ttaacatatg cgtaagagca ttttttcttg tttacaaatg
     1501 ataaatcttc atcgtttgtt gttactccag cattgtgtaa gatttttact tctttaactt
     1561 tttctattgg atcagtagca aaacaaaagt ctaattcctc gtgaacttta ggaccaatat
     1621 tgaaatacat catgttccaa agctgtgccc acatctcggc tgtccagatt tgtatatttg
     1681 ttttttgccc tctaaggtag cgatatagcc gattagaatc cagataaacc tttttccaat
     1741 aattcgcttt agggcggtta ataacccact gtgcgcctcc tgaattagtg tttatagttt
     1801 ccaaagattc tactgtaaca tttacaatgt ttgccatatc ttttagaata ttttctccgt
     1861 tttcacagct tctaatataa tcaagactta gataactaca gcagtcgcta caataccaaa
     1921 catcatcttt agaaggcaat ttgcgcaaat taattctttt attgaaaatg acatccgaat
     1981 cgatatagaa atatcggtcg tcctcacgcg aatgatcttc ttctaaatat ttccaccata
     2041 aatatggttt aatcgaagga atatactctt tgtcgtcccg cagatcatcg tacacatgaa
     2101 cttcaacacc atattccttc tcaaaaaaaa taggaatctg atcatcgtgt ctgctgaaaa
     2161 gcaatacgat atctttgatt cctagtttct tcagattagt taaacaaact tcaagctccc
     2221 atttaaaccg attgattgcc ggctgacaaa gaatatactt cattctgatc acctacgctt
     2281 gtgttgtagt tgttgtggtt gttggttttg tagttgtagt agtagttccc aaagcgctaa
     2341 tatctaatac aatgaaacta tcgttacgtt taggttgacc gtttgcatat tgtttagcta
     2401 gataaatgcg ttcgtcttca acaaaatggt attcatctga agcttcaatt tttagtgtag
     2461 atcctacacc catgaagtaa tctgaggcta ccccaataac tgcttttcct tctggcacag
     2521 ccgttgactg caaatctgaa actggtactg gcaatacttg tacgtattct ccattagcag
     2581 ttagtacagt cttagctggg aatactttag accagtaatc agttggattc acaattagga
     2641 ccacatcaga aggattcaca ttacgataaa tcggatcatt cacaccttcg atattgaatt
     2701 ttgatagtcg cgccatcaaa ccgcccatag ttacagcatc taaagctgta ataggttctg
     2761 cttttttttc agcatattct ccgctagttt gtttgctcat gtcacgcatc attccgactg
     2821 gcatatcttt accagtacca tcaacaattg cttgttctaa tgcaattctc aatgattcta
     2881 ctaaaacagt acggacataa cgatctaacc atactggacc taaatcaagc attgccttac
     2941 atacaggaat ataacctgat agcttgaact gcttcatgtt aattacatca aagccattat
     3001 ctaaaacttt tttaacagct tcgcaaagtt taccccacca tgctggattg actccacgtg
     3061 acacaatcca ttctgttaca ccagttgtgt taacaaaagt aattttttgc aatagtggat
     3121 gagattgttc taaatcttca aatacacgtt caaatacagt agctggcact aattcttcga
     3181 cccctgcaaa accttcgttt ttcactactt cgttatagaa ttttgtttct tgtgtagtta
     3241 atacacgctg accacggttc attaatacta attgatcttg attttttgct gttgcttctt
     3301 ctaaaatttt atcctgaatt tccttagata agcttaccat agctgcgcta aaagattctt
     3361 cgttaccatc tttaaaagct ttcatcaatt ggtcgcttgc agctgttaca ccttttaaat
     3421 ttttaactgt cattatttta catctccttg tccaaatgtt ttatttaatg ctgctgtaaa
     3481 tgcagcaatt ttttctgctc ttttttcttt aacgtcattc aaaatttctt caacgctttg
     3541 ttctttttta gcttcagtac ctgagctatt ttctgcatcg ataatttcat cgaccaatcc
     3601 ataactcaaa gctgtttctg catccataaa cgattctttt tcaagaagtt cttgcaatgc
     3661 ttcatctgtg ccattgaatc gtgttttata tgaagccttt accgatttat caattgattc
     3721 cagttggtca gcaatcgtac ggaagtcatc gacatttcct tctccgtatg tggaagcgcg
     3781 gtgaatcatc aattgtgcat tgttgtagat ttttatagta tcgccagcca ttgcgataat
     3841 tgaagcagca ctagcggcta agccgttaat cacaacgtta acttttgctt tatttgactt
     3901 aagtaagttc ccaatagcaa tcccttgaaa tacgtctcca ccgtttgaat taattactac
     3961 ttcaatttct tcttgatcac ctagactatc caaaatattt ttgattccct tgtcagtatt
     4021 cccttcaaag aaccaactag aaccaataaa tccctgaata aaaatttgcg gtactgcgcc
     4081 ttcattcttt actgctagaa atgttttcat tgtcgtcatt cgcctcacct cctttcgata
     4141 cttgttgatt gtttttagtt ataaatattt catctgccat cgccttatca gagcgatcat
     4201 ttccaacgcg ctctcttcct tcgttgattg taaatactcc atttctaatg cctacatcaa
     4261 tagcgtcaac caaatctttg aagctagtaa tcttgatcat agttgtatcc acacgtacaa
     4321 aattccctga caagtattct tctacttcat agagactagc gttaaacgca tcctgaataa
     4381 gttcagcaat cggtatgatt tcgaacatta aaaaagcgtc cacttgatcc gataacccac
     4441 tcatgtctcc ctttagtagg tttttcggaa cgtgaaacgc tgctgctgtc atctcaaaga
     4501 tgtcgtctat taagtttttt atatctcttg aattgctttg gaagtttccg ctgaaatctt
     4561 ctaatgtgta ctcattttgt aattgaaata ccgcacctgc attatcagct tccataaaag
     4621 ccttaaattg tgatgtcatc attttattga tttgatcttg tgttgtattg tcttgcggtc
     4681 ggaataaatt ccctttcagt acgtatctac gagcgttaga gcgcttgtaa acattcatgg
     4741 cactagaaat gagtttccca tacgcttgat aatacgcatc gactagttgc ctaatttgtt
     4801 gatctgcgta ttttatatag ataacatcac tttctagaaa ttctctatca aggactatgt
     4861 tgttaatttg cacttgagaa aacacatcat ctttcaatgc atattctgtg acatcccaac
     4921 tatccgcaat aaatatttcg ctagaattat tagacggaga aacgatcaat acttcattgt
     4981 agaatattaa tctcctgatc agtttttttc taaattctgt tgcattattt ttcttattag
     5041 gagctacatt cagcctatag taaagatcat tctttttatt ttttccatct tcatatgact
     5101 tgaattccgc tttactcatc gcatttgcaa tcaaatcaat acaagtttca atcgcaaatt
     5161 ttcgatacac aaaatcaact tgcaatttac aaaagtattc ttctaaagga accgttgctt
     5221 tttttgtgaa gtatcctacc gccttttgaa aaatccccac tttctcacct cctttcaagt
     5281 tagaatacta gaggagtaaa tccagttcct gtattttcta ctgagctatt tgtgactgtt
     5341 acaggagcag aatcataaat atcatctaaa aaattcaaac catgaaggaa tgaaaaaaag
     5401 ccatccgttt ttctagtttc aggttctatt ttttcatagc gtatatttcc attagaaata
     5461 tgctcttcat atacattcat gcaataccaa cgcataatcg catcgtcacc aaaaaataaa
     5521 cgttgattaa taaaaaggtc atcaaccaga tcttttaaca taccatgtgt aacagatccg
     5581 cttcgaacaa tttccacagt aaaacctgct tcttctaaag cgggcttcaa tatttttgca
     5641 cggtacatat ccatagcgat ttttttaata taatatttat tactcatttc aagaaaccaa
     5701 cctacaatat aatcagcttc tatatttttt ccatgaacga tctgtgattt tccttgatct
     5761 atagaaatat ctataacctc tcgtttgatg ttttgtaatc gaagggctga ttcgtggata
     5821 aaagtatgtt gtgtaaaata aacatcttta tcgtattttc ctagcaaccc aacgctggca
     5881 aaatctcgtc tatcagcaaa atcgactgtt cctatcactt catccatttt ttcaggaaat
     5941 tctttttctt tcgtatgcag aacatcatca tatgaagcaa cagcaaatcg tgtatcttcc
     6001 ataggtctgt tcattcgttt ggtcatgaac gtaagtctta aaccagcatt acgttgcatt
     6061 tgagagtatt cttgaaacat tttccgtttt aaatctgcat tgtaattaat agttggacaa
     6121 gctttttccc acatgtcggg atcatcaact tcattatcgt tatccaagcg acaaataaat
     6181 ggaaacaaac tagaaaattc tgctccatcc ttgtcaattc caagttctcc agaaagaatc
     6241 atttttgatt cttctataat gtcatcaagc ggaccaccac gaacatgacc attagttgta
     6301 tcataaaatt ctctataatc tcgaatttta ccaccaccag aagtagccac atttatcatt
     6361 gaataatctt cattttcgtg aatttcatca aagcggtttg cacctggtcg cttcccatct
     6421 tttgttctag catttgccgt gttataacga agtttgctgt ttgtagcgat attttgaata
     6481 acttccttcg tagctttaaa tactttttta tctaaatcag gatgatcttt aattacttta
     6541 aatacatcat caaaactagt ctttgcttgg ctttcattat tggcatagat atcaatatca
     6601 taatttttaa taccgtgttt agcggttagt agaaagaagt tgttccaaga agcaaaacca
     6661 gttttaccat taccacgtcc cattaatgaa agatatctat tgaacactag tgttttatct
     6721 tttttccatc gaacaccata aataaaacat tgtagaaatt tttcccacgg aattaattcg
     6781 aatggaaagt attgtgctgg tatattgatt gaatcctcta ccatctgctt atcgaagtaa
     6841 atatcttctc tagtaaagac tctttcttct agataatttt ttagcaataa ttgctctttg
     6901 cataccttga tagtgccttc ttctatagct ttgaaccaat tttcaatatg cttataactc
     6961 aggaattgat tcatttgctt cacctaccaa ttcaggagta atggcaagtt tatccaacat
     7021 caatcccatt tgtttgttga cagaaacaag caacgctact gattcattct ttttaccatt
     7081 ctccagtcta atgccgttct cggatatatc ttcttccagt gatatcgccg tttcccataa
     7141 actgatataa cgatcaacat tatctaagaa tggctcaata tttgttttct gactttccaa
     7201 ttggcttatt aaagagcggc gtaatttttc tctgtagcga ttttgagaca attcgttttt
     7261 aaacatttta gccctccttt catgataaag ttcgaaaaaa tctcttttcc tgacagcccc
     7321 ctccgtttca tcacccccaa aaaatttgcg atttatttta agggggggtt atctcaccat
     7381 cggaatgaaa gcttcagcga agtcaatgta ataattaatc tcttcaatgc tatatccaaa
     7441 aacatttttt attctttcaa cgttattatc tttattcaac gcttctctta cttgattcac
     7501 tttgtattta ctacaacagt tatctgataa cagatcccta atacctacat agcgaacgta
     7561 tatcaaacgt ttaatcaatc cttgtgtata agatgaatac tcttcaatct tctctgtgtc
     7621 atactctctg ccattgtcat tgatgatcat gcacttacca cctttcactt gcatcgaagt
     7681 tagcgaagct ttctatcttc ttctcttgtt tatccaatgc tgtaagatat ctgccatgaa
     7741 cttcattatg atgttcaaca cataaacaaa taagattatc taaatctaaa gctaagtcag
     7801 gtctatcctt gacttccttt atatgatgaa cgttctctac tctatgatac ttacctagtc
     7861 ttctacactc ttggcattca tagtgatctc gtttcatcgc tttctcccta agcctgcgcc
     7921 atttaggaga ctgatagaac ttaaccaaac gatcttctct tatcaactgt aatagccatc
     7981 tatagaattc ctcagtcatg acccgtctcc tttcgcaatc ttatttaatg cttagctatt
     8041 cttttgccat acaacggaat agcttcattg ttttcctttc gtttatatgt atcactcttt
     8101 attggtcttc tatactttcg tactatctca ccgttaccat tttgcacagt gattacttca
     8161 tacttctgtt ctaagtattg tggtctatac attgttgtta cctcctttgt gcaaaaataa
     8221 aaagaccact caacgagtga tctcatatgt aatgcacagg cagggacgtt tccgatcctg
     8281 tgcttgagtc atttgacgat tcatttgtac caaatcccaa aactcaatct aacctaacca
     8341 actatgtaat agcaacctac accgattcca tcgattacta tcgacctcgc cttgctcgtg
     8401 tactttgagc gcccatttcc aaccctcagt tgctaatgaa tactgaaaca gaataccgtc
     8461 tctgttcatg accaacgcga cagtacaaag ctttgtcgca agcaggttat cgtattccta
     8521 ataaccccaa ctaatatttc tctcctaacc tacacctgag agtggcgcac gtctgcgcta
     8581 gtattttatg ccttttaact gctagtggca tcccgactag tcagccaacc aatcggcatc
     8641 cgataagatt ggttgtttcc gtaggttcct taagtcactg gcaatgaatc gaacattgca
     8701 tggttgccga agcattgacc tagcacgcat gcttagcgtc taccctttcc gccacagtga
     8761 cactataaaa ttattcttgg ctgctactat tttttatttt gcccattttt aaatccaatc
     8821 atatagacat taagacagag cgcaaaaatt gaaattatta atggaatcat ttctcttcac
     8881 ttacctttag ttatcgtgtg aataattaaa aaaacaatag acagcaacgg atgatagata
     8941 ataagaataa tttagaagga gttgaaattc acatccttat tcttaatatt tccgctgctg
     9001 tctatcgaag cttaattaaa cgatgaggga gatttcctcc cttacatttt attttgtctc
     9061 agacctatca ctaatctttc gacactatca taatacaaca ttgaataggt aagtgattgg
     9121 tataaaaagg gtataaaatg gaaaccaaat gggtaataaa agggtataaa aaatgtaaaa
     9181 actggctact tgaaagcaac cagttctaac gatgaagcaa attgaatgat aatcttgttt
     9241 gattctagct tgacagattc ttcactcgtg ttatttcttt gagcagttac ataaatgggc
     9301 agaccattga tataacgatc atagaatatc ttcttgcgcc tttcagtcac atcaggctta
     9361 tgcggatgct gtatcgctga atagcctcga acaaacaatt tatgcagata ctcaaattct
     9421 tcttgtgctt cttctttatc gatcagcatt ctttctgctt caaatatatg atcagctgta
     9481 gaagatggaa ccaaggaata agatgctgtc acttttggtt ctcgaggttg acctatccta
     9541 catctagctg acagatatgc tgaaagaaaa acaccaacat tatgttttgt gcgatccata
     9601 tccacatctt ttgcctctgg tgtctcatac ttctttacat cgaaaagtac catccattga
     9661 ttcctccatt tatgatataa taattgtgtc agaattatta attaaggtcg gaggaatccg
     9721 gcttttttta tacccatgct tatgctaagc ttttcaagta gcgaggttgc actcgttacc
     9781 catatacatg ttgagctatc tggcggaaaa cagatggctc actatttcaa tattctgcta
     9841 aggacagcca gtggtcggct gtctttttta tattttaatg agaagcctta cttatttttt
     9901 tatctttatt acgaaaaatg atattatcta ttgataataa agcgtatagg agatggttga
     9961 gtgactatgt ggagcatgct gttattttgg attcccgttt gtattggtat cgtcgcattt
    10021 tgttactttg tcaaacactc tagaacaaat aagctcctca tgttatcttt tttacctata
    10081 gtatttttta ttgtacaaat tgttaaatat acctatattg aatttatgtg gtaggtttat
    10141 ttatctctgt ggtctttttc ataatgatac tttcctattt ttataaaaaa taaatttttc
    10201 tcttagaagt acattttttc gctgtttata tagccttcat atccactaat cgaacaactg
    10261 caatattcgc tttgctcttc gctagctctt tgtcacagtc catcgtattc tcaatacgaa
    10321 taactgctga atgattgtag acgtgttcta catatccacg aaatggatag atgaatcctt
    10381 ctgcttcgca gcgaaccatg tcaccgactt tgaattttgg ttccttacgt gttttagggt
    10441 tctttgtcgg catatctagc attaaaccgc cgatgccgtg gctactagcg taaaatccgt
    10501 cttttagttt cattatgctt cctcctcaat cgtccaagca tttgtaaatt tctctaagct
    10561 tattagttca tcttcattta aatatccgta accatataag gattctaaaa ggtcgtcttt
    10621 agaatatcct ctaaagtcaa gtctgagacc gcttaggatg tatacaaagg ttttatttcc
    10681 ataaaacttg attgtgtatg ctttcatttc tattcctcct gttctaatcc ccattgagcg
    10741 aatgctgtta gaacttcgaa ttgctgtttt tctttcagtt tgccccatgc ttttacataa
    10801 ggtaacgagc taacgcctat ttctaatctt tcatagacat cttctgtgaa actccacaac
    10861 gtatattgaa gatcatttct tttaaattca ttttttagaa aatcaaacac gatttgctgg
    10921 ttgtcgttaa gttgaattga tttttctatt tgtcttaaat cttccgattc aagtcgtaga
    10981 tgactgattt gagtcatttt agcttgctca acaattggaa accccaatcc caaactatct
    11041 tctaaatttt ggagttcgct ttctttttca gatatgagtt gttgcaaact tttcatcctt
    11101 ccgccacctc ttccactggc acagcaaacg gccagtatct ttcatcaatt gctttgattt
    11161 ctggctctgt tagctgataa gcagattttt cccaccaaca taattcaaat ttattaacaa
    11221 aacaggaatc atttctgtta ttaaacttct tgacaagaaa taaattccca atgataactt
    11281 catacaaagg ctctttctcg acctcgtagc ctacagcgta tgcttgagca aataacagtt
    11341 gtcgttcatt ttctgttttg attcgaggat tcccaaaaag ccatgtataa acttcgtctt
    11401 ctgaactgtc catcatttca tataaagttg ctcccccgcc tctgtattct tcgatataat
    11461 ccgccacaaa cttcggcaca aatggcttct gcggttcgtc tagttgtttg attagttcta
    11521 atgcgtgttc taaacccgca tattttccat catgaaaagt acttatacct ttaaagtctt
    11581 ctatgctacc tacaaggctt gctagtttat caatcaattc ctgtttattc atcgctgtcc
    11641 tccatgtatt cgtctaatat ctctttatat ttctctacaa atttgaaacg atc
//
LOCUS       DACECR010000092        11679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.092, whole genome shotgun sequence.
ACCESSION   DACECR010000092 DACECR010000000
VERSION     DACECR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.092"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(93..1004)
                     /locus_tag="IWO17_002254"
     CDS             complement(93..1004)
                     /locus_tag="IWO17_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0291113.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1405..1983)
                     /locus_tag="IWO17_002255"
     CDS             complement(1405..1983)
                     /locus_tag="IWO17_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0291114.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(2003..2671)
                     /locus_tag="IWO17_002256"
     CDS             complement(2003..2671)
                     /locus_tag="IWO17_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0291115.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(2686..3756)
                     /locus_tag="IWO17_002257"
     CDS             complement(2686..3756)
                     /locus_tag="IWO17_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0291116.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(3927..5291)
                     /locus_tag="IWO17_002258"
     CDS             complement(3927..5291)
                     /locus_tag="IWO17_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0291117.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(5288..6847)
                     /locus_tag="IWO17_002259"
     CDS             complement(5288..6847)
                     /locus_tag="IWO17_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0291118.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(6903..9383)
                     /locus_tag="IWO17_002260"
     CDS             complement(6903..9383)
                     /locus_tag="IWO17_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291119.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(9367..9702)
                     /locus_tag="IWO17_002261"
     CDS             complement(9367..9702)
                     /locus_tag="IWO17_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291120.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(9716..10750)
                     /locus_tag="IWO17_002262"
     CDS             complement(9716..10750)
                     /locus_tag="IWO17_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0291121.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(10840..11520)
                     /locus_tag="IWO17_002263"
     CDS             complement(10840..11520)
                     /locus_tag="IWO17_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0291122.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT         3348 a         2371 c         2025 g         3935 t
ORIGIN      
        1 cttaaacggt gtttaaaagc ttgcatctgc gataataagc ccggccaaac gaaaaatatg
       61 aggagctatt tttgtttggc tgggcttatt tattactgaa aataagtggt caatgcttgt
      121 gtcaaacttg cagcaatctg ctgctgataa ctgtctgtta ctaattcctt taaatcacta
      181 tcattattca tataacccat ctcgattaaa atagaaggtt gaagattttc tcgaagaact
      241 tgatagttcc cagtggagat tcctcttgaa tccagaggca gatcagtgaa ggagtcagca
      301 attgtctgtg ctaatgtttc ctcttgtcca tagtaataat atgtcgtgat tccagttgct
      361 tcattcgcat attccgtaga gtctgcgtgt aaactgataa agacatctgc ctttttttct
      421 tcactgtagt cgcatatatc tccaagctgt actaattcat ctgtagtatg ggtcaaataa
      481 accgttgctc cagcatcctc tagcgccttt tttactttta tggctgtact taatgttata
      541 tctttttctt ctgtttgttc atcattgctt aatgccccag tatcatttcc gccatgccca
      601 ggatctaata caatcactgc atcagatagc tcttccggtt ttgtttgtat attctttgtg
      661 actttggttc catcttcgag ggctaaatat ccagtatagc catcatttgt cttgatctga
      721 taagcatttt ttgtttcaga aagaaactcc gccagctctc cgccgtttat agttgcaagt
      781 ggggtagtta gcgagtcatc tgtatatagt gtacggtcat tttttccgat tttgattaat
      841 gtctcttgtt ctgtatcatc aaatgattcc tttgtctttg taaccgtact gttcgtactg
      901 ctgaccgttt gtccaactat ctttttttga ccaaaaacat ctatttgaaa acagataata
      961 atgataatga ttgcaaatac aaaagctcca attatttttt tcattcttga ctctcacctc
     1021 gtagatccag actaagggcc aaaacttaaa atcagcttaa gtaagcaaaa aaggggctgt
     1081 aaaagatata accgtagaaa ataacattac tgctaaaatg atttttttca tttttggcat
     1141 atcctgaaat ctccttttga ctattcgtgg tgattcatct acccaataaa tagtttcctt
     1201 cattctacaa acaattaatt tcttcgttat taaattaaaa cacttcctgg aacaccgaca
     1261 taataaataa aaatataccc tgataatcat gagagaataa caaatttgta tttatgaaaa
     1321 actacctata taaagaagca agatataacg atcttcgtca tatcttgctt cttcacctaa
     1381 ctattttctc cctatataac agctttacgc ttctcctaat acaccattca aatataaatc
     1441 gatcaattgc gccacattta gcggttcttc cacaggtctt ttcgaccgag tgatgtaagg
     1501 tgctaacact aagaaaaaat gccttgctgc gatttctttt gaaatcccat ttcttagcac
     1561 agattggttt gctgaaaaga tttcttcaaa aggttcacga taatttttct tccatagaaa
     1621 gaaaagatga tctcttgttt cctttgaaag attgctttca agatcgtgaa gcatcagaaa
     1681 caaatcgacc agatgtgtct caatcaaaaa ctgcgccatt tttgtcaagg ttacttgaaa
     1741 tcccgtttcg cttgtttgaa cgatttctct caaagctttt ccaacatctt ttaagtggtt
     1801 ttctaaaact gccgtatata gtttttcttt atcaccgaaa taatgataga gattgggctg
     1861 ggtgatccca actttttgtg cgatttctct tgttgaagtg ccgagatatc ccttggacat
     1921 aaacaagtca gtcgcttctt tcaaaatttt ttgctgtgta tctttgttgt atcgatggac
     1981 catttttcca cctgcttttt ggttattctt tttctttcaa tacaccatct tccatcataa
     2041 atattctatc gcatttgtca atcagccgtg tatcgtgagt aaccatgatc gttgctttat
     2101 ttttgtcttt tgattctttt gccaagatat cgactacttc tcgtgccttt tctgaatcca
     2161 aactagcagt tggttcatct gctagaataa tggacggatc attgtacaat gcacgagcga
     2221 ttgcgaccct ttgccgttct ccaccagata gttcttctgg atacttgttg atcaacttat
     2281 ccacgccaag ctgctcaaat aattcgtttg cttctttttg acgatgttca tgtttaatct
     2341 tatctactaa taataactgc ttttttactg tcagaaaagg aaccagattg gaagcttgca
     2401 aaataaagcc aatttcttta aaacgtagtt taacgcgttc gctttctttt tgattactga
     2461 attcttgatc attgataaat acagagccac tgctaggcgt ctgcaatccg cctgccaaag
     2521 tcaaaaaagt acttttacct gaaccagaag gaccgatgat tgcaacaaat tcccctttat
     2581 cgatactcat ggttgtttct ttcaatgctt taactacttg gtcgccatct tggaaacttt
     2641 tttctacttc tttaaattct attgctttca tgttcttcct ccttcttatc caattgcctt
     2701 caacggatcg atttttacga ttgtccgaac agaaaatagt gcgcctaaca cagctactac
     2761 taacatcaac aagctaatga cgccaaaaaa cagccaattg ctttggaatg gaaccgcatc
     2821 aggtagaaca agggatgttc ccactgttcc taatagacca agaagaatac caacaaatga
     2881 taaaatgaat gtttgtgcca ctacagaacg cgcaataaat cctcctgtga tcccttgtgc
     2941 tttcattaca ccaaagatat taatcttttg catcgtcagt acataaatga aaatcccgat
     3001 cacaatagcc gcaataacga tcaaaaatcc gatcataaat ccaaaagtca atacttgcgc
     3061 attatatcca ggtaattcat tgatgaattt agaaattttg atctgttcta aatcttcagg
     3121 taaattactg attttacctc tgacaactat tgcgttaatc cgtgcatttt cacttgtatc
     3181 ttctttttcg aagcggattt cttggtaagc attgatggtt gtatagagaa ctggtgagac
     3241 attgaatttg gcatgatccg taaagccagt tagtttaaac gtcttgtcac taccagataa
     3301 cttaactgta tctcctatgg ctagaccata ttcttctttc aaactaatat cgcctactgc
     3361 ctcatcatcg ttatcaaaag cttttccttc aacaagattg ggcatgatga attgattttt
     3421 atcgataccg aagaagctaa cattgatttt tccttcttct gtactgtctt tactagtcac
     3481 gactccaggt gtttgggcaa gatacgccac tttcccacct tcgacttctt ctgctatctt
     3541 cttagtaatc atggacatcc ctagatttga atttgcatct ttcgaaagca caatcgaatc
     3601 tgcctcccac ttgtcgactg ctgttcgatt atcttgagct aagccataag ctaacccagt
     3661 caaaaaaaat accaggtagg cgatcaaaaa catcacccct gcaactaacg cataacgtaa
     3721 ctttgaatgc ataatttcat ttaatgctaa aaacatttaa ctcctcctcc tgaaataatc
     3781 attttataca acaattttat cacttgataa atttttggcg aatattatgc tcaaaaaatt
     3841 cttccgctat ttcattatat gaatttttta ttctcgattg attaatattt attatatccc
     3901 cacacaataa aagaccgtga tatttctcac agtcttttat ctactctatt gctatttgct
     3961 ctcttagcta gtactttaaa agctaactca ttgaggtagt gaaaacgaga ggtgtattca
     4021 gacagaaatc caaaatcctc aactgctagc tgtggaatcg tagttttgtg tttcacgata
     4081 tccgcaagca ggttcaaatc tgataataag aattcttggc ttatcagctg tccgccaatt
     4141 atttctaatg tatccggatg gtagatcagc tttgcattta ctgttttgtt ttcttcgtcg
     4201 tattgtggaa atagatcaaa ttctcctgaa cacgaaccgg tattttgttc caagaatggc
     4261 gcttcatctt ctgtcagccc tacagttcct aaaaagtagt ctataatgac tgctgctgtt
     4321 atcttttgtc tgaggtcgta agcggttggc tgaccactca aagttagcgc tactgctctt
     4381 gccatatgta cagctcgagt aactaaaggt aaaaagagat gccgtttatt tttgtcaaaa
     4441 ctgactggga cgatatctcc aatcgcatag atgtccggat cacttgtctg cagatattca
     4501 ttgactatca cagtctcatc ctcatacaaa gccacttttt cttttaacaa tgtcgtatta
     4561 ggacgagttt gggcagaaaa aatgaccata tctgccttta ttgtttgaga cagaagttgt
     4621 acggatacta ctttctcttc tgattttttc caatcgatca agaattcatt tggataaaaa
     4681 tttatttgct tagtttgcag ctgattttga aaactgttga tcatatcctt atcgaaatat
     4741 cttgataaaa cattcggcat ataatcaact agatgaataa tgtatccttt atttctcaaa
     4801 gcatctgcta attccaatcc aatgtaacca gcaccgaaga tcactacttc ttttgcttct
     4861 tttaaagcgg caagtacttg cttagattgc actagatttt tataggtgta gacattttca
     4921 gcttcaattt gtttatacaa ggtgacagga gaggaacctg tcgctacgat taatttatca
     4981 taggttatgg tctcgttttt tccttctttg gatgcgagga tgagctgatg tttgtctgga
     5041 tcaagttcag ttactctcca ttcggtgatc agttttgccc cactttcttg aagagtccgc
     5101 atgttatggg caacttcagc taaactacgt atctttcctt ttaaataaag gttgattcca
     5161 tttgaaacat agcctagtcc atctttatga tcttcatcga tcagtaaaac ttctgctttc
     5221 ggttgaagtt ttcttagatt tagggcagct gtgatgcctt catgggaagc accaatgatc
     5281 acgattttca tgttttattc atccttaaat tcaatttttc taaataacat tcttttaaaa
     5341 attggttcaa ataggggaaa tcataattat attcgttgct agtgaataca aaaactttag
     5401 cggacttctg tgacgggtac tctttcaatt ggctagtcaa aatgatatca tactgctttt
     5461 ctttctcgta ttcgactaaa tcatatttga tcgtattcag caattgttcc tggatgattt
     5521 ctttcacgat atccgccatt agcctttcat aaggaagatc ataagcaatt tgcagacgta
     5581 attctaactg cttgatcagc gtccgtaaaa tagatgcata gcgagcaaat aattcaaagc
     5641 gggcattttc ctcgtctact tcagtaaaat caacaatagc aaatgtttgt ctgaccaatc
     5701 tttcacataa aaccaggaat tgtttatctt ctacttcagt taaccgctgt tttaatcctt
     5761 cttggccaaa aatattcatc catcctctaa aataggcaaa atgagcgtga ttttgcatca
     5821 ctgtcaacgc aatcttttgc gaaatctctt ctggaaaaac ataaaaagag aaatattctt
     5881 ctatcgattg tttaaacgtt cgattcaatt gagctactac gtcgtctttg tatttttcgc
     5941 ttatcaaaaa ctcctggaca tgcgagttat gtgtatctaa tgtaaagtta ctgatcatga
     6001 aaagataaaa aaccaacgtt tcattttgat tccaaggaaa ggcatattga aagaagtaac
     6061 gggtcaaaaa tgtatggagg tccaataaaa attgtgtcgt ttgaaacaat ccaacaggaa
     6121 gattgctata atctggtttg gattttttcg tacagcgcat tcgcttcttc ataatggaaa
     6181 gccatatttc aaatttcaat cgtccttctt ctgtaatagc aaaactaaac tctttttgca
     6241 gcagatctaa aaaagtgttt cctgataaat cactgtattc ttttttgttt tcaagatagg
     6301 gacgtccaaa ccaaaataat tggaaataaa aataacgaat ttgactttct ggaccggtca
     6361 gccgcccgct ttttattttc agatgaaact catttaaaga atcatttaat tccttcactc
     6421 tgcgagacaa agtagccgaa ctgatattaa gttccatgac aagtttctgc cgatcaaatg
     6481 agccattttc caacagataa agaagtatct gatattttgt agaatagcgc aagtaacaat
     6541 gataataaac atcgactgct gctatatttc catgtctgat taataaaata ttcccttctt
     6601 ggataacaat ttccaattgt ttgatatttt ccaagttagc ttgtaaataa aacagatctt
     6661 ctttcaaggt agcccgatca actgataagc gggtaagtac gttattaacg gaaacctctc
     6721 ctttttcctc taataaaagt tggaaaagtt tgatttgccg ttgttcattt ttttcaagca
     6781 aactgaaaag ttccaagtcc actccttcaa gcatttctga ttttaacagc agaagtttcc
     6841 ttctcatcct tgaaaaagag gatctgtgac agttatcaca gatccataca aattctaatc
     6901 acttagctgg ttggcccagc tgtcagttcc catgtgacca tgccttgata cgttgcgcca
     6961 ggttgagcat tcgtactgct atttacctct aagagtaaac catcggtatc gggaaagtca
     7021 acgagaacct ctcctttagc tgttccatca ctctcataaa tcaacgtgtt ttgaccttgc
     7081 tgaatcaatt gctttgatcc agaatgattg aagtataatt ttgttccgct taattcttga
     7141 ccttgatcat tcttaaataa agaagtcaat gtacctgtca gttgccaagg agacgtgctt
     7201 ggaagccgag tatcccgcac atcaaaagaa aaatcgcctt ttgattcata ataacttgtc
     7261 ttcaacggtt tggggagatc gttaccaaat gtaaaatcat ctggtacatt aactaatgtt
     7321 aattcttgtt tggggacaag aattgtttta gcagatgttt ggacaactgg gttaggaagc
     7381 ttagcaccat ctgctaatgt aatattgctg gaaagtacag ctgcctttgt cagtatgctg
     7441 acggattgta aagaagtatc tacagtattt aattgatagt tgtaagtaag atgaatggta
     7501 tctcctattt tgctcaatag atctgctggg acagaaactc cttgttgaat gccggtatct
     7561 gcgatagttg taccatttaa ttttaaggaa ccattgatat aagtagtatt ttcaggaagt
     7621 gagatcgtga tttttttgtc ggttatcgca tccccagatt ttgttaacgt gaatgtcaca
     7681 ttttcttgaa tcgtatctcc tgatagagca gacaggggat ctcctgtctc aggttgtctg
     7741 ctaaggtttt ttagctgact atctgcactc acagatggaa aattagccac tgttatttga
     7801 ggaagagtgg tgattccagc ttgttcagac tgccatttgt acgcaaaaga cggatacatg
     7861 ttagtaagta catcgtccac caagcgataa tcaaaataat attgccctga agccaatgca
     7921 ggtagtgtag aagtagctgt atttacaccc ccgttatttg attggatcgt ttggaaaagt
     7981 tgatctgtcc atcctgtcgc taatttttgg gttcctttcg aataaagcct gtaatggatc
     8041 gaaccctttt ttagttcagt tgctgaccaa ttggccgtga cttggtacga tgcagaagaa
     8101 aggagctttc cccactgact atcggctgtt aagtttgttg gtaaaaccgt tccgtctagc
     8161 ttgtactgct ccaatacctt taaagcaaca ggagaaagca atccgccttg cgcatctttc
     8221 ccactgtaca cgatattggc tgtctgattc aatccacttg tgcttgctgg agtatgagtc
     8281 agtattgctc cttgtgtaac gttttttcct gtaaaatagt tgactacgcg atcaaagcga
     8341 ctaaatggca actgatcaaa acgtccttca gtcagcccca tcgtcaatgt ccgactattg
     8401 gtagagcctg gaaggttctg gttatttcca tccacgtatt ctgtggtatc agcaaatgta
     8461 ttatattttc ctgttgaatc acttggtgag atgtaactat tccacgtatt tatctctgca
     8521 cttgcagaaa atgcatatgc tcctggattc aagtttccgc cagttaattt aggataaaat
     8581 ctacctgcag ctggagctaa aatcgaattg aacatacgat cttggataat attattcgaa
     8641 tcataaaaat caacagaaaa aacggccttt ttagacatag aaggttgtaa tttacccttg
     8701 gtcaaaattt tgtttggact cgtatcatat ttatctccgt tttctgtcat cttcgcgaca
     8761 ttagctgtaa catcaaattg aagcgggata tcgtccacgt tgaattcgcc tcgcgtaata
     8821 atagcaataa tatctgtcaa tagagtattt gtatcccacg accccatccc aaaacgcaag
     8881 gtatggcttc ctgctgataa taaacttccg ctagtaacct tgatgggggc accggcaata
     8941 tacaagtagg cgttgttcca gtcaattgct tttaatacat cactgcttga tacatgttcc
     9001 ggcaaattga tgatcgccac aaatgcaaat ccttgtgcag tgatattcac caaatcaagt
     9061 acatttgtta gtttcacatg gctttcccaa tgaaaacgga ccgtgccacc tttccaagga
     9121 tcatttgaac catcgttcaa ataagtagca gtcaatttag gcaattggtt agccccagtt
     9181 agttggacaa gcttcccgcc attgactgca ctacgtccac catttggagg tacgatattt
     9241 tcatacgtgt atggatcata agaaatggca ggaggcgttg ctccaacagc ttctgccgaa
     9301 ggtgaaaaga taaatatact taaaacaaaa agcgaagtaa aaaagaagga gcgaaaaaat
     9361 gaaaatttat tttgtagcaa catgttcatc ctccttttcc tttcttctcc ataacaacaa
     9421 ccacgcaaga agccataatt ctataccaat gatcaaataa agaaaacgga tcttttgttc
     9481 attcgtctcc gggaatacgc catgttgatt agattcttta taaatcggta gctgtgtctg
     9541 ctgttttgat gcgccattga ctgaaccact tggatgcgga attggactta gatcataaaa
     9601 tgtaatcgta cctgaacttt tggtatctgc ttggactggc tcttgccagc tgaagaacag
     9661 caaacttgca ataatccctc ccaataagat cgatgttttc aacgaaatca cctcatcatg
     9721 ttgtttctcc ttttttcttt ttcttgtgga cccacaaaaa cagcaaaatc aaccataaca
     9781 ctagaactgc agcaaccaat cctgcataaa gccatgggaa agattgttct tgtactgcaa
     9841 aaacggaatt ttcatttact ttttgtgctt tttctttttt gatcgtaaag ttttgtgtaa
     9901 atcgccattc attactgtcg cctgattttg ctatgatttc tgcgcggtaa tcgccggctt
     9961 ttgctttatc accagctaaa tcaagattca agtgaaaatt cgaatttggt gcaactaaaa
    10021 atgcattaga cgtattttca atataaagct gattttcata aaagatcttg ctcgtaaata
    10081 tcaaattgct gataatccta ggctgaggat tttggaaacg aagctgaaca gttggctttg
    10141 cgttgacctc gtccaaatct cctgataaaa gtttcagatc cggttctatc gtctcttgac
    10201 tttcttgtaa aacgactgcg agattataag caatacgatt tttgatttgt gctccttctg
    10261 tagcattttc tgtttcagct tcttctttag gtgaaacatg aatacccgct aatacacgtc
    10321 ccttaaagct ttttgctggg attttcactt ctataggtac agttgtttct gcatgtgctg
    10381 atacattgat ttcctttttc gcaatgagca aaacgtcacg gacatcaaac ggcaatgtct
    10441 catctaacgt tgggtttttt tgactataat cgatcgttcc accatcactc gttaccgccg
    10501 gattgacaga gacctcaaat gttcgttctt catctgaaga attgctgata attacttcta
    10561 gtgtctgatt ttgttccggt gccactaata aatcaaaata accgattgct ttatctattt
    10621 ggttctctgg aaataccggt ttgattccaa agtctcctgc tgaatccttt gcttggctga
    10681 ttgacggaaa aatgaaaaat agacttaaaa tagaaaaaaa cgaaacaaac agtcgtttac
    10741 tttgattcat tgtccaccac atcctttcaa caacaactgc ggtaaaaaat ccttcaccta
    10801 tagtattttc accgcagttt gttgaattta tttcatactc tatgctactg ggccagccac
    10861 gaggttccaa gtcaaatcag ccgtgaatgt tccagctgct gctttcggca ctttcagtgt
    10921 gatatttgct ggatcataaa cagaaagcca tgttcccaga ccttgatctt ttgctgctgt
    10981 aaatattggt gtcgctgcac ttgctttact caacccagtt acagtttgac tagttggtcc
    11041 agtggattcg ttatctgacg tacttttaac agtggatgtt ccaaaatcta attcagcgcc
    11101 ttgcaaaact tgtgaaccgt ttacaaaatc tgaaaccgaa actgtcacgt tccacccttg
    11161 tgcttggcca tctgcaccta cacctcgacg atctgatact tggagataag gtgtatcgtt
    11221 ttttgaagcg tctacttgat acgtttttgt tccgctttca atctcgtgtg taccaaatgg
    11281 caattcggga actacatcca gtgttaaagg acctgtctgg ccagttgctg gattttccgg
    11341 atctgctggc tctactggtt ttgaaggatc agtagggtta agaggctcca cggtatttgt
    11401 tggttcagta aaggtaatat ctccttttga agtcgcttga ccctcagcaa aagctggtgt
    11461 tgccaagccg cctagaagaa gtgttgaaag taataatgtc ccaatcatgt tctttttcat
    11521 aataaatcac tcctttttct tagtgaccta agtatagtag agacagtatg caaaaaatcc
    11581 tcatgaattc atccgcttac ttgaaattat gacccttttt gtataaaaga gaaaaaaaca
    11641 ataaaatcgt ttacatgaat attcttcctt ttttattaa
//
LOCUS       DACECR010000093        11667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.093, whole genome shotgun sequence.
ACCESSION   DACECR010000093 DACECR010000000
VERSION     DACECR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.093"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(133..2031)
                     /locus_tag="IWO17_002264"
     CDS             complement(133..2031)
                     /locus_tag="IWO17_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ0291123.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(2192..3454)
                     /locus_tag="IWO17_002265"
     CDS             complement(2192..3454)
                     /locus_tag="IWO17_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0291124.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(3447..4346)
                     /locus_tag="IWO17_002266"
     CDS             complement(3447..4346)
                     /locus_tag="IWO17_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0291125.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(4491..6269)
                     /gene="uvrC"
                     /locus_tag="IWO17_002267"
     CDS             complement(4491..6269)
                     /gene="uvrC"
                     /locus_tag="IWO17_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ0291126.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(6451..6765)
                     /gene="trxA"
                     /locus_tag="IWO17_002268"
     CDS             complement(6451..6765)
                     /gene="trxA"
                     /locus_tag="IWO17_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ0291127.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(6852..9212)
                     /locus_tag="IWO17_002269"
     CDS             complement(6852..9212)
                     /locus_tag="IWO17_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0291128.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(9327..9875)
                     /locus_tag="IWO17_002270"
     CDS             complement(9327..9875)
                     /locus_tag="IWO17_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ0291129.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(9933..10370)
                     /gene="zapA"
                     /locus_tag="IWO17_002271"
     CDS             complement(9933..10370)
                     /gene="zapA"
                     /locus_tag="IWO17_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ0291130.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            10572..11489
                     /locus_tag="IWO17_002272"
     CDS             10572..11489
                     /locus_tag="IWO17_002272"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ0291131.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
BASE COUNT         3309 a         2476 c         1971 g         3911 t
ORIGIN      
        1 tcatgtttgt tgaatagtgt gacttgtgac cgaggagttg gcagaagaac accgtttatc
       61 cggaaaaaga gactgcgaca cgacttttgt ctcagtctcc agtctatgga acaatgttca
      121 ctggaattct tctcaatcgt cctttcgata gtccaacagc cttttgagct gggtaatctg
      181 ctgctgcagt tccatacctt ttgttgtatc acgattgagt ttgcgtggta gttgctgttc
      241 gattactttt ttcacatttg tttggtcgga tttttttgca aattgttgtt caataggcaa
      301 aaacggataa tgtgtcacga tttggaaacc ataagaattg cataatagtg aataaccggc
      361 aattcctgtc gttttttgat aagctttgga taaacctccg tcaatgacaa atacttttcc
      421 atttgctttg atcggtgact cccctttttt tacttttact ggcgtgtgcc cattaatgat
      481 tgctgaccct tcttcttttg tgtcgaattc tgctaaaatt ttttcactga accactcttc
      541 atctcgcaag tgataataag gattgctttt ctcgacgtgc gtatgaggct ctgtgagaaa
      601 ataacgttca aaagtcgcca ttttactctt gccgaataaa ggcgaaaatg gcccactcca
      661 cgcataccaa atcaaatcgg ttgctaaatc ttcttttgca gtcagatctt ttgcgctttc
      721 agttatctgt ttttcaaaaa agcccagcag ttcttttccg cgataagatt ggtctaattc
      781 aaatgctaag agctgtccct tctcgtctac aggtatacag ccatgaaaca gcacatgctg
      841 attgttcgtg agatacattc cccctttttc gatcaaaaac gtcatatgtt tttgcattct
      901 caaagagtgc tggaatgaat acatcaaact atcaatcact ttttgttcct cttccaagaa
      961 tttttttgga tcatctggct ggatcgtttg aaaacaagtt cctttcaact catagagctg
     1021 accttctaaa tcaatcgttg atgcttcata gtcgattttt tccagcaatt tacgatcgtc
     1081 cattttaaat tcaggtcgac gatccaataa ctgatgttct gatttgaatt gaaggataga
     1141 aagtgcctga tggatttttt ctaattcttc ttgctctctt tcggatagat ttttactgtt
     1201 tttaggcgca aatactggat tcttctgata agtttgatga gcaaaagaaa acaatggacg
     1261 taaattcaac ccatattctt tctctaattc aaataaataa ttatagcgtg ctgcaatccg
     1321 taaaagtgtc agtaaacatg cctcagagcc gcaatacgca ccaatccaca aaatatcatg
     1381 atttccccat tggatatcta cagaagaaaa atccatgatt cgatccatca ctttggctgc
     1441 ttgagttcct cggtcaaaaa tatctccaac gatatgcaaa tgatcgatga cagatttttg
     1501 gatcgtctgt gcaagtttcg ttaaaaattt ttcttcttcc ttcatcaaaa caagttgttc
     1561 aatgattttt tttacatagt ctttttttcc attgatacgg tcatcgatat acaagagttc
     1621 ctcaatgata tagctatatt ctttcggcaa tccttttcga acttttgacc gggtatattt
     1681 gactgtcgta aaccgtaaaa gatcgatcaa atgatggatc agctgcgatt tatcttgttc
     1741 tttgtaccaa tcagtcgtta atgcatattc tggataagcc gtaaaaatag tgaattggct
     1801 gatatccgtt tccgtcattt gttcagaaaa aaggtctttg atcttttctt taagatttcc
     1861 tgaaccgata cgcagaatat gatcaaaagc aggaaattcg ccatgtatat cactcaaaaa
     1921 taactctgtt ccttttggaa gctgcaaaat tgcttcgaga ttgatgattt ccgtgatgac
     1981 atcctccgtt gaggggaatt tttctgaaag taaatctaaa taacggtcca ttaaaacgcc
     2041 tccaaaaatt ggtatactca atattataaa gttgttatac actattttca atgaatatat
     2101 atccaatata gtaaaataaa taaaataggt ctatacaaaa agaagctggg aacaatagat
     2161 gtccagcttc cttctgtatt ttaggattgt tttatgcttt ttttcgttca ttcctcataa
     2221 ttgaaatcga ttgtttcaat gaactccata atccgccttc gctataaacg agtacattcg
     2281 atttgtacat gtttgcagac aagatcatca ataagaatgt tacggctaca agtaccaaca
     2341 aggaaatcaa tgctccaccc ataccgactg tctcatttgc taagcgaatc ggcataatat
     2401 atgaagataa gaatggaata tacgaagtga tccgaataac gatattgttt ggatctgaag
     2461 ctcctagtat caatccaagc atgtatccgc ctagagatag ataagtcact ggcaaaattg
     2521 cttttgctgt atcttctgct ttgttgacca aagagccaca taaagctgct aaaacagaaa
     2581 aaatcaaaat gccaaagatc atgaatagaa gagaatacca caagaatggt ccaatcaagt
     2641 tatccaatga gataccgtct aatatatttt tgaccatttc catatctttg aattgactgt
     2701 atcctatacc aaaaataata ccgtataatg ccatttgcgt taatgcaaca agtaaaacac
     2761 ctgttaattt tccgtaaaaa tgcttttgtg cagtcgtact ggacaggatc acttccatga
     2821 tccgcgtgcc tttttcagaa gcgatctctt gtgcaatgat ctgcgcatac gtcaaaataa
     2881 taatgaacaa gacaatcgtt gctacataac ttacgacgta ttggactgtc gagttatctt
     2941 ctccgactgt catttttcct tttgaatcaa aattgacttt ttgtcgagtc aaacttgctt
     3001 gttggctcaa gctagctact tcttctggtg aaatgcctaa ttgactcgct cgaagcatcg
     3061 attgcaagcc agtcagttgt tgttgtatca gtagttgtgt actttgtcct aatgaatttt
     3121 cgctataaag catcccattt atcttatcat tctcagttga gactactaaa tacgcatcga
     3181 ttttttcttc cgaaagctct ttttgtgctt tttcttcaga atcaactgct ttgaactcaa
     3241 aatcatctgt tttgacttgg ctcatttgct gactgatttg ctcattatca gccaatacgc
     3301 cgattttatt gacatcacta ttttgttgag caaagtttcc tgctacataa atgataccca
     3361 gcatgataaa agggactaat atcatgatca agaaagaaat cgacttaaca ttttttaaat
     3421 agacatcttt ggtaataatc caaaacttac gcatcgattt cacctgcttt caatttgaaa
     3481 atctcttcca aagttggcgg ttgctggtta aacataggaa tgtagccgaa ctgagtcgca
     3541 cgagtaaaga tttctttccc agcttcagga tcttctaagg taatctctaa cgtattgtct
     3601 tcatgaagga tcactttttt cacgccttcg atttcttcta cttcctgttg gcttaaacca
     3661 gattccaaga ataacttcgt acggccaaat gattcacgaa tctcatgtac ttttccattc
     3721 aaaaccattt ttccgtttcg caacatgatc aaatgatcgc agattttttc tacattatcc
     3781 atattgtggc tagaaaaaat gacacatgaa ccatgttttt tcaattccaa tattccatct
     3841 tttagcaatt cagcatttac cggatctaac ccgctgaatg gctcatccaa aatgatcaat
     3901 ttaggttcat ggattaacgt ggcgatcagc tggacttttt gctgatttcc tttagacaaa
     3961 gattttactt tatccgtctt tttccctttt acctgaaatt tttccatcca aaagtcgatt
     4021 tttggttcaa tctcttttct tgatttccct ctaagttctg caaaataaag cagttggtct
     4081 tgaatcgtta ctttcggata aagtccccgt tcctccggaa gataaccgat gacatcataa
     4141 tctttctccc caagatgatg gccattccat aacacttctc cttggtcttg tgtcaaaaaa
     4201 tccaagatca aacgaaaagt cgttgtcttc ccagccccgt tttggccaat tagccccatg
     4261 atttttccat cggggatttg gaaagagaca tgatcgacag ctgtgtagtt cccaaaagtt
     4321 ttaacaagat ccttgacttc taacatttct tcactccttc ttcaagaaat cacatatatc
     4381 cttagacacc agacgtaagc gatttattat ttaccaacaa aacgtaaacg ctctttttgt
     4441 tgtacataaa atgataggac actttacttt ctttgtaaag tgccctttcc tcattcctga
     4501 tgtaatttat caaatacgtt ttgtgcgaca tttttcggca gtccgatttc ttgtaattct
     4561 tctgtcgtgg ctgctgtgat atttttcaac gatttgaact ctttaagcag catttttttt
     4621 cgttttggtc ctaacccttc aatttcatct aatcgggaag caaaactgtt tttactgcgt
     4681 agctgccgat ggaaagtgat tgcgaatcga tggacttcat cttggattcg ctggagtaag
     4741 aaaaactctg gtgaatttcg ctcaagcggt actacagcca aatctgtacc gaatagcaac
     4801 tcatttgttt tatgtttatc gttttttacc atgccagcaa taggaatatc gattcctaat
     4861 tgattttcta agacatcttt tgccacatct acttgtcctt ttcctccatc gatcaagatc
     4921 aaatcaggta aaggcaaccc ttcgcgaatc actcgcgaat atctgcgata gatgacttct
     4981 ctcattgatg cataatcatc cggtccgttg actgttttta ttttatattt ccgatattct
     5041 tttcttgccg gttttccatc aatgaatacg accatcgcag ccacaggatt cgtccccatg
     5101 atattagagt tatcaaaaga ctcgatacgt attggggtcg ggatattcat cgcgtttcct
     5161 aaacgttcaa ctgctccgat cgtccgttcc tgttttctgg caatcagatc aaaccgttca
     5221 ttcaaagcaa ctgaggcatt cttactcgcc aattcaacta attttttctt ttctcctcgt
     5281 tttggttgca gcactttcgt agctaacaaa gcttctacac tgggcttgtc gatcgtatca
     5341 ggaatcaaga cttctttagg aatgaaatgc tcattttcct gataaaactg accgaggtaa
     5401 gtcaagaaat cttcttctgc ttcgttatag aaaggaaaca ttgaaacatc tcgttcaatt
     5461 agctttcctt gtcttacgaa aaacacttgg acacacatcc atcctttatc aatcgcataa
     5521 ccaaatacat ctcgatccag taaatcagta tttgtcattt tttggcgagt catgattgtc
     5581 tcaatggcac ggatctggtc tcggtattct gcagcttttt caaactccat gttttcagca
     5641 gctttttcca ttttctgatg gatctctgac tcgatttttt cgtgtcctcc gtttaaaaag
     5701 tttttcactt catcaacgat tttcgtatag actttcggat ctacgtcgaa ataataagga
     5761 cacaggcatt gtcccaaatg atagtaaaga caaggctcct tcgtctgact aggtttgcat
     5821 tttctcagag gaaataatcg atcgaggatc ttctttgtct cattcgccgc accgacatcg
     5881 ggatatggtc caaaatacag tgctttgtct tttagtacct tacgagtaat caccaaacgg
     5941 ggatattttt cattcgttat cttaaggaaa ggataggttt tatcatcctt taacataatg
     6001 ttgtatttag gatcattttt ctttatcaga ttgatctcta agagcaatgc ttcgatattc
     6061 gattctgtaa cgatgtattc aaagtcttcg atttcactaa caagccgttc cgttttcgta
     6121 ttatggctac cagtaaaata tgagcggaca cgatttttta atattttggc tttgccgaca
     6181 taaatgatcg ttccattttt atctttcatc aagtaacagc cgggctgatc aggaagtaaa
     6241 gctaatttgt ttttgattcg ttcgttcatc tggtactccc cttcatattc aattaaagtt
     6301 attatagcat gtctgtcaaa aaaacaatcc agcctgacaa cgtaattata ctcaaaaaat
     6361 attaatttga ataaccttaa ctcaaccgca aaaaaaccga ggacggaact ggattcagtc
     6421 ccaccctcag cttatttttg ctgtaagttt ttataaatat tgatcgatca tttgacgtag
     6481 ttgttctttt gaatgaacac caacagcttt ttctactact tgaccatctt ttttcaacat
     6541 taatgttgga atgctcatga taccgaattg ttgaggagtt tctggatttt catccacatc
     6601 cattttagca attctgaatt cttcttcatc atattcttgt tccaactgat ccaaaatcgg
     6661 tgcttgcata cgacatggac cacaccaagt tgcccaaaaa tcaatcaata caagtccttt
     6721 atctgtttct tcattaaatg tcttatctgt aattacttgt gacatatcaa agacctccat
     6781 ttttaaaatc tatgactagt atagcatttc tatacttgga tagcgaacca tctgcttacc
     6841 tttagtaagc tttacttaaa tttaacaatt gtcgcaccat ttccgccttg gtttgccggt
     6901 gcaaactcaa agcttttgac gctccgatgg tttttaagat aatcagtaat tcccttgcga
     6961 agtgcgcctg ttccttttcc gtggacaata gtcacttgag gataaccagc tagaatggca
     7021 gaatccaaat attgatctac ctcacttagc gcttcttcgt atcgttttcc tcgaaggtcc
     7081 aattgattcg gcacgtgact actagattca gatcgtacag catggataac tcgttgtgtc
     7141 ggctcttttt gaggagcaac aggcgtcagc tcgtcttcgg acacattcat ttttaatata
     7201 ccgatttcta cttgccactg attcccgttt ttacgtaaaa gcgtccctct ttggccgtaa
     7261 gtcgtaacca acacttcgtc tcccggtttc aacgtttttt gttctttcgc cttcttcaat
     7321 actttatttt tcttcaaagt ttcttcttga tgtagatttg ctaattgggt ctttgcatcg
     7381 atcaactgat gctctttcac atttccttgc ccaatctgct gttgcatttt acgaatatca
     7441 gcaatgattt tttctgcttt ttcttccgcc tctgacacga cttcatttgc ttttttcttt
     7501 gctttttcca tttctttttc acgttcttca aagaaatagc tgtaagcttc tttcaaatca
     7561 tcgtgaagtt cttgtgcctc agaaacaaaa tgacgcattt ccagatattc ggtctctgcc
     7621 attttccggc gattctctaa atctgtgatc atttcgttta aatcttggct ctcatcattc
     7681 atcaactgct tcgcttcatc aataacttct gtatcaagac ctaatcggct agagatttca
     7741 aacgcattgc ttcgtccagg aacgccaatc agcaaacgat acgttggact taatgtatcc
     7801 acatcaaact ccatgctggc attaatcgta tttgcccgat tatatccata aactttcaat
     7861 tcaggataat gcgttgttgc catcacataa gcagactttt taccaagatc atctaagatt
     7921 gcgattgcta gagcagcacc ttcttgcgga tctgttcctg caccgagctc gtcaaataat
     7981 accagacttt tttcattgac ttttgataaa atatctacag tgttcgtcat atgagaagaa
     8041 aaggtcgata gactttgctc aatcgactgt tcgtctccta tatccgcaaa tacttcctca
     8101 aaaataccga tttgactttc ttcacctgca ggaatcggta atcctgcttg ccccatcagt
     8161 tgtaacagac ctaaagtctt caacgtgatc gttttcccac ctgtgttagg tcctgtaatg
     8221 acgatggctt gatagtcttt tccgattgtg atgtcattag aaactacttt ttcctgatcg
     8281 atcagtggat gtcttgcttg tttcaagacg acatgattgt cttcactgat tcccggcacg
     8341 atggctttta cttctttacc aaatcgtgct tttgcattca tcagatccat tttaccaatc
     8401 acataagtat tgtggatgat ttcttgacga tggggaacaa gttcagcaga aagttcactt
     8461 aaaatacgtt gaatctcatt tcgttcagcg atttggtatt gacgcaaacg attattcaaa
     8521 tccaccactt gttttggttc aataaataaa gtctgacctg aagcactttg atcgtggact
     8581 acaccgccaa atacaccgcg gtattcttgt ttgactggga taacgtagcg gtcatttcgc
     8641 atggtgatga tcgcatcact taaatacttt gcatttttcc cacggacgat cccatctaac
     8701 tgttcacgaa cagcttgttc acttcgccga atgttctgtc ttatgctctt taactcagga
     8761 gaagcgtcat cagtcacgcg tccgtcttca tcgattgctt cctttaaacg gcggctcaaa
     8821 acagggatcg tgactaactg atctgcccac atatacaaac gtgcaaattc gatttcactg
     8881 tcagaaagat catcaatgaa acgattcaat tcgcttgtcg tagacaatac tctggaaact
     8941 tgtgcaagct ccactccatt caagtctgct ccgatctcaa tccgtttcat atggggacgg
     9001 atattttcaa ttttaggtat agggatcccg cctcgcagac gttgcacttt caatccatcc
     9061 tcagtttctt gaagccagtt tgtgatcgtt tgtttctcac tcactggtac caattctgcc
     9121 agttcttctt ttccttgtgc agtcactaca aattgtctga ccatctgttt gactttttca
     9181 aattccaatg tttcaaggat tcggttattc attttatcac cttatctttc actatgaaaa
     9241 aagagctgtg ccggacatac gctacgcagc cttcttaaat aaactactct gtacaaaaaa
     9301 cgaaatattt tccagcacta ttttccttaa ttcaaaatat tcgttaccca aagctcatac
     9361 atcttgttgg taagaaatgg cgttctctca acgataaaac gagcgaaact atgctcgccg
     9421 aattgatttt ggaccacatc taatggaaca aaggcaagta actgcaaaat caaaaatata
     9481 gtaacgtaag ccatgattag tgacaggatg cctcctgcaa tccagtctac ctgtctcaag
     9541 atcggcacat acgtcaatcc atggacaaaa acccccacaa atcgggtaag cagccagcca
     9601 attagcaaaa taatcagaaa agcagttcct gcataaaaag cttcatctag gtgaaaggca
     9661 aatacctgat caaaaaatgc taactttgat gttggggtga cagctgggta tggaatgtat
     9721 aattccaaat gacttgctaa tggtttgtaa aaatgctggg cagcaataaa tgacaataca
     9781 tatccaatcg cgaatatgat ttgcaaagca aacccgcgac gtgctccaga aaaaaacgca
     9841 attagcaata aaaataaaat cagcaaactc agcatgctac gttcctttct cttctaactt
     9901 ttttaaattt ttcttatttt gataaaagga tcttaattag aagatttttg tttaatttgt
     9961 ccttttcgtt tctcgtttaa aatctgttgt gcttccacat gatttttaat ggtatgatct
    10021 gcttgaccat tttctttcaa tacttcccga gcttcgttct cgattgcttc aatgcgtttc
    10081 acacgatttt caagctctgt aaatttgatc atttttttct ttaattctgc tgtttcttct
    10141 tccagctcca atattctttc ttgtttcttc aattgatccg aaagcgcatt gacagccatc
    10201 aagattgctg cttgttcatt atccatttgt ggtgataatt gtttcaattc tgctaactgt
    10261 tcattgatca gcttcgttac gatatccata tgatgcttgg tttcccgtcc gataatagta
    10321 tatgtttggt tggcaatcac cgctttgtag cgtgtttttt cgtgcgccat acgaatcatt
    10381 tcctcctatt tgatgaaagt tttatttagt gtaccatgaa gttggttctt tcaccgttct
    10441 ccacaaatat ttttcacata cttaactatt atatgataaa ctttaaaaaa atgctatgtc
    10501 tcgcaatctc ttagaaaacg aaaagtcgtg agttgcggta tagttttatg tataaaaacg
    10561 aggagaattt tatgagcaac gtcattatca aagcaacaaa cgaccaactt agtcaaatga
    10621 aagcatacta tcgctcttat ttgttaaata aacagattcc ttatactagc tttgctgcaa
    10681 aaaaagacgg gacgaccatt accgcttata catccggaaa agtgatgttt caaggtacga
    10741 atgcagcaat agaagctgat aaatgggggg aggcacagga taaacaagcc aagccagcaa
    10801 aaacagcctc ttctttgcca aaaaatatca gtcaattatc cgttttaggt agtgatgagg
    10861 tcggaaatgg cagctatttc ggtccgctaa cagtatgtgc tgcttatgtg aaacgtgatc
    10921 agcttgcaga attacgtaaa cttggtgtta gagattcaaa agagttaaaa gatagccaaa
    10981 tcatccagct tgctgacatc ttaaaaaaaa ccatccccta taaactgctt gtcttatctc
    11041 cagaaaaata caatgagatc caaccaaaat acaatgccgt tcggatgaaa gtcgctttgc
    11101 acaatcaagc gatccatttg ctgctaaaaa aaatcgagcc agaaaaacca gaagcgattt
    11161 tgatcgatca gtttacgccg gaagcaaatt ttaaaaaata tgcgcgttta gaaaaaaatc
    11221 aattacaaga aaaactgtac ttcgttacta aaggagaaca atatcattta gctgttgctg
    11281 ctgcttctat catcagtcgt gccagctttt tggaagagtt agacaaagca tcagctgaag
    11341 ctggaatcac ccttccgtca ggagctggaa cgaaatctga ccaagtagcg gcaaaactat
    11401 tggagaaagg cggcatgcct atgcttgaaa aatatgccaa acttcatttt gccaatacag
    11461 aaaaagcttt gaagctacta aaaaaataat ggaactgtga caaaagtcat gtcacagtcc
    11521 cttgttccga ataaacggtg ttcaatcatc tgatcctcgg tattagctca acaaacggca
    11581 aaatatgagg aactattttt ccgtttgttt ctctaatact cagaacaaaa cgttgatttc
    11641 acaacctttt tcttaaacgg tgttctc
//
LOCUS       DACECR010000094        11591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.094, whole genome shotgun sequence.
ACCESSION   DACECR010000094 DACECR010000000
VERSION     DACECR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.094"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            107..970
                     /locus_tag="IWO17_002273"
     CDS             107..970
                     /locus_tag="IWO17_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0291132.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1099..2721)
                     /locus_tag="IWO17_002274"
     CDS             complement(1099..2721)
                     /locus_tag="IWO17_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0291133.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2970..3491
                     /locus_tag="IWO17_002275"
     CDS             2970..3491
                     /locus_tag="IWO17_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291134.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IWO17_002276"
     CDS             complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IWO17_002276"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ0291135.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5572..6390
                     /locus_tag="IWO17_002277"
     CDS             5572..6390
                     /locus_tag="IWO17_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ0291136.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6493..6690)
                     /locus_tag="IWO17_002278"
     CDS             complement(6493..6690)
                     /locus_tag="IWO17_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291137.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6859..7437
                     /locus_tag="IWO17_002279"
     CDS             6859..7437
                     /locus_tag="IWO17_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0291138.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8482..8985)
                     /locus_tag="IWO17_002280"
     CDS             complement(8482..8985)
                     /locus_tag="IWO17_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0291139.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9344..10369
                     /locus_tag="IWO17_002281"
     CDS             9344..10369
                     /locus_tag="IWO17_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0291140.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNLMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10692..11570
                     /locus_tag="IWO17_002282"
     CDS             10692..11570
                     /locus_tag="IWO17_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291141.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         3589 a         1972 c         2257 g         3773 t
ORIGIN      
        1 ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct
       61 ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt
      121 aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt
      181 atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa
      241 ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa
      301 tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata
      361 tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt
      421 gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat
      481 tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga
      541 agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga
      601 cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag
      661 tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga
      721 caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat
      781 cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga
      841 gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt
      901 aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat
      961 cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa
     1021 cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt
     1081 tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt
     1141 ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc
     1201 aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga
     1261 gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt
     1321 cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca
     1381 acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa
     1441 caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata
     1501 ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg
     1561 taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc
     1621 acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac
     1681 cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt
     1741 tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc
     1801 aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat
     1861 ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa
     1921 ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc
     1981 cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc
     2041 aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac
     2101 aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat
     2161 atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg
     2221 tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa
     2281 ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc
     2341 attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa
     2401 atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc
     2461 catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac
     2521 ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga
     2581 taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt
     2641 tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact
     2701 cacatcattt acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt
     2761 cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt
     2821 tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg
     2881 atttattagt ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa
     2941 ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct
     3001 cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg
     3061 gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg
     3121 aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa
     3181 taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag
     3241 tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat
     3301 ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca
     3361 atattttagg aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga
     3421 cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg
     3481 catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt
     3541 tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt
     3601 cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg
     3661 aagcatagcg cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat
     3721 ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta
     3781 aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag
     3841 gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg
     3901 taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata
     3961 caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat
     4021 tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca
     4081 tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta
     4141 gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg
     4201 tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga
     4261 catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct
     4321 ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt
     4381 ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga
     4441 caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc
     4501 gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg
     4561 cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt
     4621 catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag
     4681 gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt
     4741 ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg
     4801 ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg
     4861 ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa
     4921 cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta
     4981 ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga
     5041 aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt
     5101 gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca
     5161 aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg
     5221 catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt
     5281 cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt
     5341 aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt
     5401 ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta
     5461 tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt
     5521 ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa
     5581 tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt
     5641 atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta
     5701 aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta
     5761 gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt
     5821 gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat
     5881 tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt
     5941 gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt
     6001 gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta
     6061 ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa
     6121 ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt
     6181 gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt
     6241 gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag
     6301 cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt
     6361 atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata
     6421 gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa
     6481 tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag
     6541 ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag
     6601 gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa
     6661 gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact
     6721 atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact gatttttact
     6781 tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg
     6841 gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc
     6901 gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag
     6961 gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg
     7021 atttattgga acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc
     7081 ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg
     7141 atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc
     7201 gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag
     7261 aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat
     7321 gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc
     7381 gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat
     7441 gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc
     7501 gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact
     7561 gattactagt tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt
     7621 agttggtgtc gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg
     7681 taatctgatg ttctatctat ttaagaaaag catgtttaca gcagtatgat tctgttgtaa
     7741 acgtgttttt atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa atagatagtt
     7801 agtttttcca cagtggataa cttcgtaaga gataaaaata aaaaattagt caaagaaggg
     7861 cgtatagatt tcttttgata ggagatatac aacagaaatg aataaatatt aacgtgtttt
     7921 caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacact
     7981 tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta
     8041 ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca
     8101 gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat
     8161 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
     8221 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
     8281 taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt
     8341 ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt
     8401 tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac
     8461 ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc
     8521 tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt
     8581 tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt
     8641 attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc
     8701 tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa
     8761 attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg
     8821 taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta
     8881 agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag
     8941 aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat
     9001 ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac
     9061 caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg gtttctttaa
     9121 caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc
     9181 ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat
     9241 gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat
     9301 cttggttcag ctttttttcc aattactagg aaggagggat agattgttca agacttattt
     9361 tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt
     9421 tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt
     9481 agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat
     9541 gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt
     9601 tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg
     9661 attattccat atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac
     9721 gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt
     9781 cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac
     9841 aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc
     9901 tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca
     9961 taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc
    10021 gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc
    10081 attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata
    10141 tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatct
    10201 gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata
    10261 tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac
    10321 ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata
    10381 tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa
    10441 aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa
    10501 aaattttata tttattctat caaaaaattt tgtttatgat ggtaactgaa taaactttcc
    10561 tgagatggtc aatgctttcc ttgaaagtct tacagatatt ctttcttcat agaaagttta
    10621 agttttatag ttagtgtaca ataaaagtat gtttatacca tatggtgtcc ggataatcag
    10681 tcatttaaac aatgaggttc aaagatatga aaaggggaaa tttgatgaag aaaataagca
    10741 atgaaatcca aattctggca gaaactttaa ctcttcccaa agtcgttata gagcaaatga
    10801 ttgtacaagc aaaaaacatc cttgatgagg tgtttgagca atatagtcat gaactaatgg
    10861 atcctaataa agcaaaggcg actgcaaaga aaatagacaa agtctatgaa gaggaacctt
    10921 ttgcagcatt agctatttat ctatatgcag cttctcaaag ttggaaaaaa atttatcaac
    10981 cattgaagat tccaagaact atctatttgg caacaatgaa tgcttttact agatttattc
    11041 atgaacattt ccaagtaaca ggaagctatc gctttgaccg tggtttttgg atttggcatt
    11101 acactagtgg cttgatcttt cggattggtg aattggagta cgaaatgaca tattttccga
    11161 aagggtacac ggatctaaga ttagaaggaa agcagtgcct ttctcttcat attccttccg
    11221 atgcagattt atctcagaat aagataagag atagttacga aagtgccatt cgtttttttg
    11281 aactttattt tcctgattac cattaccaag tgatgtacac agatacttgg ttactatcac
    11341 ccaatcttac taaatggttg aaaaaagagt caaaaatctg tctttttgca gccgattatc
    11401 gattattatc agttgatgaa caggacgata gtggagtccc ttggatcttt ggacgagtag
    11461 atgcccaaat acatgattat ccagaaagta cgagtttaca gcgacaagca aaagagcaat
    11521 tgcttgcagg cgagcacatt ggctctggct taggtattct tacttcataa gtttagtact
    11581 tcgaaatagt t
//
LOCUS       DACECR010000095        11494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.095, whole genome shotgun sequence.
ACCESSION   DACECR010000095 DACECR010000000
VERSION     DACECR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.095"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..232
                     /locus_tag="IWO17_002283"
     CDS             <1..232
                     /locus_tag="IWO17_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291142.1"
                     /translation="IAQQQAEQARQQSAAEQASQQAQAAAPEQNNEQTVYVTPTGSKY
                     HTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(394..1566)
                     /locus_tag="IWO17_002284"
     CDS             complement(394..1566)
                     /locus_tag="IWO17_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0291143.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(1772..2587)
                     /locus_tag="IWO17_002285"
     CDS             complement(1772..2587)
                     /locus_tag="IWO17_002285"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0291144.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(2737..3183)
                     /locus_tag="IWO17_002286"
     CDS             complement(2737..3183)
                     /locus_tag="IWO17_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0291145.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(3180..4184)
                     /locus_tag="IWO17_002287"
     CDS             complement(3180..4184)
                     /locus_tag="IWO17_002287"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0291146.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(4224..4553)
                     /locus_tag="IWO17_002288"
     CDS             complement(4224..4553)
                     /locus_tag="IWO17_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0291147.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(4679..7006)
                     /locus_tag="IWO17_002289"
     CDS             complement(4679..7006)
                     /locus_tag="IWO17_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0291148.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(7849..8142)
                     /locus_tag="IWO17_002290"
     CDS             complement(7849..8142)
                     /locus_tag="IWO17_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ0291149.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(8545..9723)
                     /locus_tag="IWO17_002291"
     CDS             complement(8545..9723)
                     /locus_tag="IWO17_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0291150.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(9827..10141)
                     /locus_tag="IWO17_002292"
     CDS             complement(9827..10141)
                     /locus_tag="IWO17_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0291151.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            10248..10463
                     /locus_tag="IWO17_002293"
     CDS             10248..10463
                     /locus_tag="IWO17_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291152.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(10763..11005)
                     /locus_tag="IWO17_002294"
     CDS             complement(10763..11005)
                     /locus_tag="IWO17_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0291153.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(10992..11420)
                     /locus_tag="IWO17_002295"
     CDS             complement(10992..11420)
                     /locus_tag="IWO17_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0291154.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         3261 a         2249 c         1980 g         4004 t
ORIGIN      
        1 aatcgcacag caacaagcag aacaggcgcg acagcaatca gcagctgaac aggcctctca
       61 gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa actgtctatg tcacaccgac
      121 cggttcaaaa taccatacac ataaatgtgg aaatggtacg tatacccctg ccactttaag
      181 cgaagctcaa gctcgtggtt taaccccttg ctccaaatgt tatggaaact aattcaaaag
      241 ttatactata ataataaaat tcttctgaaa cataaaaaaa tagaagatgg cgttccagat
      301 agtaaaccaa aaataacagt ttacacgtcc atttcgtcag cttctctctt ttttcattta
      361 gtctgttccc tctgcttttt tggacatctt cattcattct ttctcaatca aatagtacac
      421 ctctttttct tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa actgtttcga
      481 atgtttatgt tttacatgga ttttaagcag gagctcctca tgttgcaagc gatagttcct
      541 gccttttaaa tcagaaaatc tcactagtcc gtattcatca gaagctttca caaatggaac
      601 tttttctcta gtaatatagt gtttgccgtt gagcgttttt acagaaaaga ccattccttc
      661 gattggtcgt ccgtttgtgt tctttacata aaaagataaa ggatagtttc gcttagcgat
      721 ggccaaagaa tctaaataag cgatgagtag ccaaactaag ccgattgatc caagtagtat
      781 accgataaac caaataaaaa atttccattt tttccattga tcaattcctt ggatcgcttt
      841 ttttgcctct tttgcttgat aaggaatacg atgccccgtc acaagcagtc gatgtgtatt
      901 gaccatataa ggagtacagg taagcaaagt cacgagatct ctgccttttt ggatacccaa
      961 agaatctact tcatcaggta agaccacttg gattttttct acttgatacg ccaatgtctt
     1021 tccattgatc tggatgaaaa actggtctcc ttttttcaac ttaggcaaat ctgtaaataa
     1081 ttttgcttct gggagtccac gatgcccaga aagaacggca tgggtgtttt tcccgccagt
     1141 cggataagaa ctttcttcca ataatgacgt tcctttttgc aaaaatattt ccgttgtctg
     1201 atcgaatata ggtaagctga cattgatttt tgggatttcg atgactccaa gcatatgttc
     1261 cataaaaaag gcattactag gtaaatcttt taatgctttt gcatcaactg cttggttgaa
     1321 actgcctatc cctggtgata cgttttgctc tgccaactgt tggtttttct ttgtcatttt
     1381 ttcctgctgt ttttttattt cctctgaatt cttattcgat gcttgctttt gataatggat
     1441 gatcatttgc tgggcaagaa aatcattcag tgaatcttct acaaaaggat aagaaaagat
     1501 cccgatccca acgactaaaa tgattgccat gaagatgtcc agtattctta ttctgctttt
     1561 attcatcatg tccacctatt ttctaatatg tatatgaaga ggttgtgaaa tcagcgcctt
     1621 gacctgagta ttagcgaagc aaacggcaaa atagttcctc ctattttgcc gtttgttgag
     1681 cgaataccaa gagaccgacc acttgtcaca acctttctcc ccatttgaat cactatcgtt
     1741 tatttatcgt atgatttttg tttccaatag tttaagcttc tgtttcttga tggcgttttt
     1801 tgaaatagat cactgctcct ccaactaaag cagtacctac caagatgaat acgatgatcc
     1861 ccataccacc agttgaaggc aagattcctt taggttggtt atcgatcgtt gtttcatcag
     1921 aagttgttgg atcatcatct actgtaaatt cagtgtacgc agcttcattc ttaggtaatg
     1981 cgtagccttc tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc agcccttcga
     2041 ttgaaaattt cccttctgaa tcagaaatca atttcacttc ttcccagtca gaacctgtga
     2101 cttcatctga ccaagcatat gggtattctc ctgaggcaag gtttgctatt tttttcatta
     2161 atttaacagt tggatctttt gctcttagaa tagaaaattc tgcatcggct aatttttctt
     2221 gcgtgttcac atcttgtttg atgaagtgga aatcaccgta gttttggaca ttttctacag
     2281 taatcgtgtt atccgtagtt gtattttccc gattgactac accgtttgga ttcccaagat
     2341 tggcttcatc tgcgccatgg atgataccgg ttgtttgttg caattttccg ttttttacta
     2401 taaaagtagt ttctttttgt ggattggtta attgataacc atctggtgct tttgtttcaa
     2461 ctaaagtata attgccgtct attaatccat tgacactgat ggatccttca gtatcagttg
     2521 tgaatgtcaa cgctgactca gcaggatcat agagagcgac accagtgttt tcatcaaaac
     2581 cggtcacgaa ttgattctct gaatttttga tctggaattg cgcccctgct aacttatcta
     2641 cccctgaaaa gttcccagct ttctggattt tgatattgct ttctttgatg acatttttag
     2701 catataggtg aatcgtgtct aaaggtttgt cagggtctag ggtaagctta acacgatatt
     2761 tggcgacgtt tcgacaccgg gctttggtgt ttctacaaag acgtatacag catctttccc
     2821 atcagattta cctggaagag tcaggttagc taatccttca ccagaagtcg ttacacttgc
     2881 gaagggtgta gcgtctgctg gaactgctgt gtctttactt ggatagtacg tagcagtgat
     2941 gtcatatacg ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg tcaattccgt
     3001 tccgttgttt atttgatttt ctggtgctga agtgaatttc aatttatgca aattaaccgt
     3061 aacattttct ggtgtaggtg atacggctgc agaaacctct gcagttccta ggccgagaac
     3121 gaaaggaagc agcatgatag cagttcctaa aaacgtccat atttttgttt tgatgttttt
     3181 cattttattt ccctcctatt gtttcctttt tgttgttttt tataaaacca agctgtccct
     3241 gttgaaagga ctagcaataa ccctaaaatt gagaaacctc caagcatctc ttcgcttgtt
     3301 tttggaaagc ttcctccatt tttttcattt gatggaccat ttgtctgcat ctgaactggc
     3361 gtttttccat tattcgtcgg atttgctttt ggttcgttga tgatttccaa tggtatagga
     3421 tgaagcggat cataactgcc tttttcaact tcaaagagaa tggttgattg actcaatata
     3481 tatcctttag gcggtttaat ctccactagt tcgtaacttc caaaagacaa tccatggaca
     3541 gaaaatgttc catctttact ggaagtaaaa atcgttgctc ttgaatcatc tttggctacc
     3601 caatgattta tcccgttttc ttgcgctaag tattggtcat tttttttgac tagaaattga
     3661 gcatctgcta gtttttgttt cgtttgccga tccactttga caaattgttt gcctcctgtt
     3721 tggatggcaa gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac atcgttatca
     3781 aatgtcgttt tttcactgtt ttctttcaat gtcatttggt aacggatcgt taattttttt
     3841 ccagcaaacg aactaagatc ttttccagaa tctgataaga caaaaccatg ttttattttt
     3901 tgtgtgtgaa atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt ttctggttct
     3961 agccataact gaggatcagc tgtatctttg atcgtatagg ttcctatctc gttgatgttc
     4021 tcaggaattg ttacggtgat gagataaggg actttatccc catctgcaaa atctgtctgc
     4081 ttattctcta ctgtttttgt taatgtgagt gcagcaggta ttttttgttc gctctttgga
     4141 tacaagtgga tggtcgtcaa tttatgatta gatgaatctg tcacaggaag cacaacgaca
     4201 aatggtgctg atttttcttt tacttaatct gaagtcttta tttccgtaaa tcggtaaaca
     4261 gcgtcacgac cttgctcatc tttgtccgat gcactaaaag aagcaatgcc ctcttcctcg
     4321 tctactgttt ttgtcacatt ttctgccagt attttttcac tatcactttt ttgtgctagt
     4381 tttctttgtg cgtcttccac actacttcct tctgaccgca gcttgtaaaa ttcatccgag
     4441 acatcatata tagcgaaagt aacgccgttc aaccctttta ctccttgaaa gttatccggg
     4501 atatttattc cgtcgttttg actgatactt ggcatcttat cgctttcgaa cattcgatta
     4561 tgcaaagtaa atgtaatagg tgattctcta gctgacacag tcggagaacc tataaaaaag
     4621 accagtagga taaaaaagag aacagcaaat atatccacac gtacattttt tgtttttttc
     4681 atcgctgttc acctgctttt ttcttacgat ggataaaata aaagaagcca gaaaaaataa
     4741 agatcagtcc taaaaagaca aagggcagta gacctatccc acctgtcgaa ggaagcaaga
     4801 gagtcggatt gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa ttattgttta
     4861 cacttccttc gcctaaacta atcgttacta tttgtgatcc aatatctgtc cggctgttaa
     4921 ctgttagggt ataatccaat gattcgccta agacaacacc ggtaacgtgg agatttccaa
     4981 ccgaaatcga gtgactttgg gcatcataat taaaggcagc gtcatcgaca gtaatcactg
     5041 cgttagcttt atagatggta taagttcctg atgtgttggc tatatcagta aatgacaact
     5101 tgggatttgc tgctttgact ttgtctattc cgctattcgt tagtttaatt gtatagctgc
     5161 cagcattaac catatttttt gctgattgtg catctgctgt gtaatccata ctgctaagtg
     5221 ccacttgtac tggagtaagc gaactagtga caccctctgc tagcttgaca tggattgacg
     5281 ggacacctgc ttttccatca tatgtcttac tgccactgag ctctaccgca ccgctgattt
     5341 gcttaatcgt atagacatca gatgtctgca cgatagtaaa taaatagttt gttctggcag
     5401 ctagtttagc tagtccgctt tttgtcaatt gccaagacgt ataggttcca atttcagtag
     5461 gcgcagttac ttcaactccc ttttcattca tcgctttgaa atcagttgga ttccatgtga
     5521 tttgatcaat actgagatca ttgtttaaaa gggatgttga ttctgtttta tcattaaagg
     5581 catatttaac gatcggtaca acagaaagac catcatagta cttactgcca tttgcattga
     5641 ttgatactgt tgcatttgtc ttataatata ggatgacttc agaaatctgt tttccatttg
     5701 cgtcctttac gattacattt ccatttgtat ctaaactagc taaaacactc attgctgatt
     5761 ggttctttgt ttctatcgct gaaacagctg taaaggtata tccgttgata gtaggcgtat
     5821 tagtcgtcag gtcttgtgca gcacttccgt aagtaagatt cattatttta gaaggagcaa
     5881 tcatctgtcc aaaatagtct tgatacttaa tagtgattgg tgtttgtttg cctgtatagg
     5941 tcaccgtatc gacgttactt tctagggctg tgaaagaacc gtttgtttgt gcaaaagaat
     6001 agcccgttac tgctgttgga gtgataacca ctttgtcacc cacatggatt ccagataatg
     6061 tagtcccgta cgttccgatt tttgctgctc caccattgac tgtataagaa aaagttcctg
     6121 tccgattcgc attgacctga accgttgctg cttgataggt tggaaaattt gaaacatttc
     6181 ccttaccatc aacgatctgc ccatttttga tcgagtaccc cgtgaatgga atatcatttg
     6241 ctaactgtat accgattgaa aaagtactca tacctcctcc ggcaggaagc cctccccatg
     6301 ttgagggtgt aaaagaataa cttttatccg cattgggtgt aactgaatcg atcgtatttc
     6361 cagcggtagc cacccccata tagatacttt ttgggaatgt gatagtagta ccatttactg
     6421 tggagccaat aaacagatta tggatatctg gacggttatc aataaaagcg attgctttgg
     6481 ttacgccttg taacgcagtt ttatccgcac tcatcgatcc gttaaccgca acaatgactc
     6541 catctttggc atatatcact gtatatccat ctggtatggt atgtccgttg aaattcgcag
     6601 tggccgcaac agtactaaat gtctggcttc tacctgtttc tgaataagct ttctcccctg
     6661 ctgcttttgc tgctgtgatt tctttttctg tcggatcaat cagttgagtc gaggatgtgg
     6721 ttgaggttgt ctcgttgttg ctagactgat tggtgctttc tatggactgt atttcagaat
     6781 agggtggact atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt tctgtataaa
     6841 cgtttacata aatacttgat aagctcccta agaaagtaaa aatcaagatg atcgaaatca
     6901 taatgggatg taagtatttt cttttagata cagctttgag tctagaaaca aaatgttttt
     6961 gacatatacg ttggatttta ttttgagttt tcttaatcat tttcatccct cctctcaata
     7021 aaaaatcatc aaataatcct ttacacgtta ttctaaattt aatttttatt attttattga
     7081 tgtatagaaa gtttggggct ttttttctct ttttattcta aggttatttt agtcttgtcc
     7141 tttaatacaa agattttatt tttaaataaa aaatactctt tttttacgat tattgaaaaa
     7201 aaaagataaa acttgattaa ttgaagtatt gcttaaattt ggaagctatg agggaagaat
     7261 aaaagagtta ggacgggatt atctactatt tttctttgtt ttaaatattt gttatctttg
     7321 aacataaaag agaaatgtgt cgactgcagt gttgtgtccc tcatcacgaa taaacggtgt
     7381 tcaactagct gatcttgtat tcacttaaaa aatggtaaag tacgagttgc tattccccat
     7441 tttcaatttt ctaacgctca ggacaaaata ctgttttcac aacctcattg gaataaaagc
     7501 atatatattg tttcaagaac gaaatcttgt tatcttttta aagggcatct gtccaaaatc
     7561 taattttagg aggtacgttt tggtatttat tcgtacaaat aaaagagaat tatctttatt
     7621 catgtaacgc atccaattga ttttttcatg tacgctcctg gaacgatttt actgacgatt
     7681 gcttatttaa ctgcgaatcg ctcattagct tttatcatag ctatccatat tttgaataac
     7741 gttcttggct ttgtattata gatacagatg acaaaaaaag ttcaagtatg cggtgtagaa
     7801 ccaatatcgc atacttgaac ttttttcaat gagtataaat taaaacttct aattttcaat
     7861 tcccatcatc tgatatgccc gaccgtctga acttcttttg aaaaagccaa atgtgattag
     7921 ataacgtctt tgttcgacat aatcgccact agaaaatacg ttcagcagta tcgaattaac
     7981 ctctgtttct gtataagtca ctccatggtc aaagcgtttc aagacttctt tcaataggat
     8041 gtatttcttt tttgttgaac ttggtacttg ttttactttt ccatcattca tgaatgtgtc
     8101 taaaactttc atcttttctt gctccgtata agtataaacc atcttctttc tccttttgat
     8161 tctaagaata ctccatttat gttagaatat tctggatttt ttattaaagc ggttactctt
     8221 aagaaagata ttatcatagt gctctttttt ttactactac ttataggtag atttattcta
     8281 atccattatt ctcccctacc aataattaaa gaaaaaactg gatcagggtg ttcaaattac
     8341 atgctaagaa aaaagaaccc ttgaatacca agggttcttt ctcagtctga taacaatgat
     8401 taacgacgga tttctttgat acttgtgaaa agtcgttaaa gtattgatat aaaaggattt
     8461 ttattacctc tgactacata ttgactaatt tgttaaaaaa tcgtttttca agaatcctgt
     8521 aataccagtg ctttcaccgt ttttttaact ctctaaagaa ctaaaagcaa tcagaggttt
     8581 taccttttca tttgacgtta agtggcgata aattttcgaa gttgtttggt cagtatggcc
     8641 taagcgatct ttcaagactt cccagctaca gtgttccgct actaagcttg ccattgtatg
     8701 acgagctaaa tgagtatgaa atggcttatt aattcctgag cgattcacgc accgtttgac
     8761 gtgatcattt acatactctc gacgataagg aacaccacgt ttactagaat caagatacac
     8821 aaacaaatat gtttcttcta taataaacct gtaatgttgc ctcatctcac ccaaagcctt
     8881 aatcaactgt tgtagctttt ccataactag aggagttact agaactcgtc tagcagaact
     8941 aactgtctta gtagtttcaa ttcgaaaatc cttactctta cctttaactg ccgtttgctt
     9001 atagaacaga atttcatttc ttttgaaatc tatatctcca ggctgtagag caagcgcctc
     9061 gctgactcgg cagccagtgt aaaatagaac atggaaaagt acagacatcg gtaattcctc
     9121 atgaacaatg ccataattca aaaaagttct caattcatcg acagtaagaa acttgtcctc
     9181 aatattattt atggcttctt ttaaatcagt taccgttaac tgatattttg gcattctcac
     9241 agacttcatt ggattttcac ttatcaaatt ctttctttga caatatttaa aaatcatatt
     9301 tagaacttga ataagtgatt gtaaactatt tttggcatag ccaacatttg ttccattaac
     9361 gccacattcc tttaaggtaa aaagcaattc ttgaaggcac tctggtgtca aatcagacaa
     9421 ctttttatca ccaatccatc gaacaataat ttttaattgt tcctctcgct tatcataggt
     9481 tatttccttt tgttcattta acgaacggta atattggaac cattgctcac ctacaacact
     9541 aaacttgtca ttgcttgcaa caattttttt cttaagcctt tgttgtttct ccatttccag
     9601 ataagcgagt tgcgcctcac gttttgaatt aaagccacgt cttgttgttc gatatgcttg
     9661 aaagtaccat gtgatcctac cagtagaact gatttgcttc ttgaatcgat ctgttttttc
     9721 catttattga ttcctcctat tttgaaggaa cccaacaagc agaaaccctt tggaaatctt
     9781 tctatctatg tcatcattgt accgcatctt agaaagaaaa tgtaacttat aaagatcgaa
     9841 gtagtcgcaa tttttctcga tagtcattcg aatcactctt tctcatccat tctaaaacat
     9901 catcacgaaa atagtgtcct cgaatcccgt accaacaggg aggaaactca ccaagtttta
     9961 ctaaacggtt cagtgtacta tcacttatct gaaatagtcg ctttaaatca gtatttgtta
    10021 ataccagagg taaatgactg actgcaagca atttttccgt gtaatcttcc agtttttttt
    10081 gtaaaattga atagaactct atttccagag aattaacatt tgttttaact tccaactcca
    10141 tcacccttct ctctcaaaat caaaacgacc accacggtac cccttaaaat tgtaaaaaaa
    10201 cactccagta ctccatcaaa acggactaga aaagaggaat ttttaatatg aaacaaggaa
    10261 ttgcagacat aaaaataatt aaagaaattc ttgaaaaatc tacggctaat gcaatagcat
    10321 tcggaacagg aatcaacttg agtacagtaa aaaaacttaa aagtggagag agagctgaag
    10381 aaaaattaaa tcttgccgat gctattaaaa tcacagaatt tggcatgaaa aatatgccaa
    10441 ccaaaatcga aatatggaaa tagtagtttt tctgatctat cagctgtttt gccaaagcac
    10501 ataggaatct cacctccccc cagtaatggc gggccgattt ggttacggaa gtatcattat
    10561 cgtagtcgcg attctcatat taaaatttgt cgcttaaaag ctaaccttgt catggcgtaa
    10621 ctagagaatc aaaatctaca tgtactgata gatttgctat tcatttgtca aagagcgcta
    10681 ggctattttc agcctatgga aatcctttat tttgacccga taaaaaactt ccatagaatg
    10741 aaaaggaaag ggaaaaaata ctttaacgag ttgtatcaaa ttcaagtatc ttctcaatca
    10801 attttgtttc cagttgtcta cgcatatatt catctacata cataaccatt cctttttctt
    10861 cttcataaac tggtttcatg gctaaccgtg aaatataatt ttgatatttt gacagaacga
    10921 tggacatggc aattgaatca ccagaagctg cggctttgat tgtcgttagt ggtatgaatg
    10981 gtatatatcg tttattttgc ttcatatttg acacccaaat actttcctag ctcttccaaa
    11041 gccatttgtc gttggtacca aacactgccg agacttttcc caataatatc gccaatttca
    11101 cgatcattaa gaccgatgaa gaaggacata aaaatgattt cttgtctttg actaggtaat
    11161 gctgcaatcg catttgccaa ctcctcattg gaaataaaga ttttcatacc aaggacaata
    11221 aaagtaccac tactattcaa atttggttcc aaataataga actctgtacc taaccctttt
    11281 tgttccaata tttccattga tatttgccgt tggcgaaatc tagcgtactt cttttgaata
    11341 tttaacgcct cattgcagac tactttctta caaaaactgt caaacgtatg ttgaatgtgg
    11401 gtttcctctg agtcgcccat aatattctcc ccctcaaaaa attcattcaa ctcgttttac
    11461 ttctatccct ttccattatt actacaatct agga
//
LOCUS       DACECR010000096        11235 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.096, whole genome shotgun sequence.
ACCESSION   DACECR010000096 DACECR010000000
VERSION     DACECR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.096"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            369..998
                     /locus_tag="IWO17_002296"
     CDS             369..998
                     /locus_tag="IWO17_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291155.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFGLIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(1122..2231)
                     /gene="ugpC"
                     /locus_tag="IWO17_002297"
     CDS             complement(1122..2231)
                     /gene="ugpC"
                     /locus_tag="IWO17_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ0291156.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            2440..2862
                     /gene="mgsA"
                     /locus_tag="IWO17_002298"
     CDS             2440..2862
                     /gene="mgsA"
                     /locus_tag="IWO17_002298"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ0291157.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            3185..3721
                     /locus_tag="IWO17_002299"
     CDS             3185..3721
                     /locus_tag="IWO17_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0291158.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(3761..5524)
                     /locus_tag="IWO17_002300"
     CDS             complement(3761..5524)
                     /locus_tag="IWO17_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0291159.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(5527..7242)
                     /locus_tag="IWO17_002301"
     CDS             complement(5527..7242)
                     /locus_tag="IWO17_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0291160.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(8021..8245)
                     /locus_tag="IWO17_002302"
     CDS             complement(8021..8245)
                     /locus_tag="IWO17_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291161.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            8445..11213
                     /locus_tag="IWO17_002303"
     CDS             8445..11213
                     /locus_tag="IWO17_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0291162.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
BASE COUNT         3545 a         2057 c         2186 g         3447 t
ORIGIN      
        1 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
       61 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
      121 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
      181 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
      241 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
      301 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
      361 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
      421 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
      481 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
      541 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
      601 cattgtcaca tcttgtgttt gggttgattg gggcttggta cttgcaaaag catccgagta
      661 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
      721 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
      781 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
      841 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
      901 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
      961 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
     1021 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
     1081 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
     1141 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
     1201 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
     1261 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
     1321 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
     1381 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
     1441 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
     1501 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
     1561 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
     1621 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
     1681 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
     1741 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
     1801 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
     1861 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
     1921 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
     1981 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
     2041 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
     2101 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
     2161 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
     2221 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
     2281 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
     2341 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
     2401 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
     2461 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
     2521 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
     2581 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
     2641 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
     2701 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
     2761 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
     2821 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
     2881 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
     2941 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
     3001 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
     3061 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
     3121 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
     3181 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
     3241 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
     3301 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
     3361 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
     3421 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
     3481 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
     3541 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
     3601 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
     3661 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
     3721 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
     3781 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
     3841 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
     3901 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
     3961 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
     4021 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
     4081 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
     4141 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
     4201 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
     4261 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
     4321 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
     4381 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
     4441 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
     4501 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
     4561 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
     4621 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
     4681 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
     4741 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
     4801 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
     4861 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
     4921 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
     4981 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
     5041 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
     5101 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
     5161 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
     5221 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
     5281 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
     5341 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
     5401 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
     5461 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
     5521 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
     5581 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
     5641 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
     5701 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
     5761 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
     5821 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
     5881 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
     5941 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
     6001 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
     6061 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
     6121 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
     6181 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
     6241 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
     6301 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
     6361 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
     6421 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
     6481 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
     6541 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
     6601 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
     6661 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
     6721 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
     6781 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
     6841 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
     6901 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
     6961 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
     7021 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
     7081 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
     7141 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
     7201 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
     7261 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
     7321 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
     7381 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
     7441 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
     7501 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
     7561 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
     7621 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
     7681 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
     7741 ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa aaagaaaaca
     7801 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
     7861 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
     7921 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
     7981 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
     8041 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
     8101 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
     8161 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
     8221 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
     8281 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
     8341 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
     8401 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
     8461 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
     8521 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
     8581 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
     8641 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
     8701 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
     8761 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
     8821 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
     8881 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
     8941 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
     9001 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
     9061 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
     9121 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
     9181 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
     9241 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
     9301 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
     9361 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
     9421 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
     9481 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
     9541 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
     9601 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
     9661 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
     9721 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
     9781 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
     9841 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
     9901 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
     9961 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    10021 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    10081 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    10141 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    10201 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    10261 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    10321 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    10381 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    10441 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    10501 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    10561 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    10621 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    10681 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    10741 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    10801 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    10861 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    10921 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    10981 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    11041 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    11101 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    11161 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    11221 caagttgttg ccact
//
LOCUS       DACECR010000097        10937 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.097, whole genome shotgun sequence.
ACCESSION   DACECR010000097 DACECR010000000
VERSION     DACECR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.097"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..154
                     /locus_tag="IWO17_002304"
     CDS             <1..154
                     /locus_tag="IWO17_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0291163.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(235..1218)
                     /locus_tag="IWO17_002305"
                     /pseudo
     CDS             complement(235..1218)
                     /locus_tag="IWO17_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1613..2455)
                     /locus_tag="IWO17_002306"
     CDS             complement(1613..2455)
                     /locus_tag="IWO17_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0291164.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2780..3196
                     /locus_tag="IWO17_002307"
     CDS             2780..3196
                     /locus_tag="IWO17_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0291165.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="IWO17_002308"
     CDS             3193..3663
                     /locus_tag="IWO17_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0291166.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="IWO17_002309"
     CDS             3677..4450
                     /locus_tag="IWO17_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0291167.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="IWO17_002310"
     CDS             4464..5276
                     /locus_tag="IWO17_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0291168.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="IWO17_002311"
     CDS             5330..6295
                     /locus_tag="IWO17_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0291169.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6432..9155
                     /locus_tag="IWO17_002312"
     CDS             6432..9155
                     /locus_tag="IWO17_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0291170.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9168..9800
                     /locus_tag="IWO17_002313"
     CDS             9168..9800
                     /locus_tag="IWO17_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0291171.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10005..10232
                     /locus_tag="IWO17_002314"
     CDS             10005..10232
                     /locus_tag="IWO17_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAQ0291172.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10301..10567
                     /locus_tag="IWO17_002315"
     CDS             10301..10567
                     /locus_tag="IWO17_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0291173.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10557..10826
                     /locus_tag="IWO17_002316"
     CDS             10557..10826
                     /locus_tag="IWO17_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0291174.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3626 a         1742 c         2137 g         3432 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcaa gatgcagcga ttaaatgact atctttatca actaacattg cttttgaaat
     6481 ctacgccttc agataattat tcagctcgca gtatttcgca agctctacag ttgaatagaa
     6541 gtacaatcag tagctactta aatgaaggtg ttcgtgaagg gcttttcata aaagtaaaaa
     6601 gttacccggt tttattttta catagaacag ctcttgaaga actacatatc acactgaata
     6661 actccgaaat agaatcaatt gaaagcttgt taacaatttc tcaaaaaccc gcattagatc
     6721 aagtaattgg ctctaaagga agtttgaaag aagcaattga tcaaattaaa acggcagtct
     6781 tatatcctgg aaaagggttg cctttattat tgatcggcgc tagtggatca gggaaaacct
     6841 ttctagcgaa taaaatatat gaatatgcag tagaagaaaa ggtcattcac acatcggcac
     6901 ctttcataga ttataattgt gcacaatatg tgagcaatcc agagttacta tcctctgctt
     6961 tatttggcta cacgaaagga gcatttaccg gagcaagcca ggagcatact ggtttgctag
     7021 aaaaagccga tggaggagta ttgtttttag atgaagtgca tcgcttatct gaagaagggc
     7081 aagagaaatt atttacattt atggatacgg gtgaattctc tcctatgggg gataatagta
     7141 ttcggaaaaa agccgatgtt cgattagtct ttgcaacaac agaaaatata tatacgactt
     7201 tcttaccaac ttttcttagg agattgccag ttattgttaa tctacctaga tttcaacaac
     7261 gtccgtcgtt tgaacgtctg tcattgattg atgaattttt tgtttctgag agccaaattt
     7321 tagctaagga actatctgtt tctgatgcat tgattcattt cttaatgaat agtgattttg
     7381 aaggtaacgt ggggaatatt aaaaatatta tcaagtatgc ttgcggaaac gcatatatcc
     7441 accagaagag tcagcaaaca attcaagtta aattattgga tatgccttta gagtacagtt
     7501 ataaattcaa ggagcaacta aataaaccca gaaagaaaag agcagatcga atctactatc
     7561 cagaagaaac acgacaagtg caactagaaa gaaacaatca gctttttgtt caattttttg
     7621 accaattact tactggattc tctgaagtga ttgaaaataa caaggatatt aaactatttt
     7681 tagaagaaat ggttaccaag gtaacacaag taatggatac gcttattttt caagaggatt
     7741 acgaaaaaga gcagtcttta tacgccattc taaactatca tattcgtcaa agtattgatt
     7801 ttatgcgaga aagctacggg tttgaacaag atggtaaccg ggtagtcgct ttggccaact
     7861 atctatatgt caaggataat caagtgattt ttgaaaatag tcttcaagaa aagaaaacca
     7921 aagatcagct taacgaattt tttgctaatg ttcttgaaac cccttattgg tatgcaaaaa
     7981 aaatcttaag ccttttatct gagtgtttag atcaacctgt gtatgaagag gacattttct
     8041 ttattacgct atatttttat agtttgaatg tcacgaatgt taaaacaggt attaagagta
     8101 ttgtgctagc tcatggttat tctacagcta gtagtatggc gaatgttgcc aaccgtatgc
     8161 tgaataagaa cgtttttcaa gcttatgata tgcctttaga tattacatta gagaaaattg
     8221 aagcaaaatt gattcaattc cttggagatt atcgaacaga ttcaggcttg attttattgg
     8281 tagatatggg ttcactaaac caactaggcg gtttattagt gaatcatcta aatggaccac
     8341 tgttaatcat tgatcatatc aatacaccgt tattattgga agtcggccaa caagtattaa
     8401 acaataaaag tatcaccgaa ataggagcag aaattgaaac aagtcttact ttcaaaaagc
     8461 agttactact tcccaagaaa aagaaaaaga aagccatcat tacttgttgt tatacaggga
     8521 ttggtagtgc gacccagatt caggagattt taacgaaatg cttgaaagat gcagttagag
     8581 aattagtaat tattccgtat gattacaaaa agctgttaga aaacaagaat tttgagactc
     8641 cttttcaatt atacgatgtc atcatgattg taggtacaga agatcccaaa attaatcaga
     8701 ttccatatat tggattagat cgcctgatta atgggggaag cattggcgaa tttgttcatt
     8761 acttggaaaa agagctgact attgaagtga acgatttaca aaaagaatta attttcaatt
     8821 tttcaattag taaaattgtc gaaaatttaa ccattttgga tccagataag gtgctatctt
     8881 ttgtacaaaa agcaattaaa gaattatcga agttattaaa ttttgatttt tctaataatc
     8941 agctgttttt gttgtacctt cactgtagtt gtatgattga acgaattgta cgtaaagaaa
     9001 gtgtggatga gcaagaggac attgaagaat atatgcaagc aaatggagaa ataatggata
     9061 aaattcatag cgcttttttt gagattgaaa gggaatacac aattgaaata ccagatttag
     9121 agttgcgatt attgagcgat attgttcaag gataggagtg aattcgaatg acaatgggag
     9181 taatttttga tatggatggg gtactcatag atagcgagac attttatttt gagagaagaa
     9241 tgaacttctt tcaagaactg gagatagatc caggatcaaa ccagattgat gattatgtgg
     9301 ggaaaacaga agctgcaata tgggaaacat tagttcctga ggacaaatca ttaagagagg
     9361 aactttatcc gaaatatgta gattatcgaa aaacgcatcc aattgatttc gctaaagctt
     9421 tacgaaaaga agtgaaaccg ttacttgact tcctcaagaa gcgggatata ccattggcac
     9481 tggcatcttc ttctccagct gtcgagatag agagtatgct cattcaatgt gaacttactg
     9541 aatatttttc ctttgttata agtggtgagt cattaaaaca aagcaaacca catcccgaaa
     9601 tatatttaaa agcgaaacag caattagact gtgacttctt tattgcagta gaggattcaa
     9661 cgattggtat taaatcagca aaggcagcaa atatctatac ggcggcacta aaacaaccaa
     9721 tgcaaatcga tcagagtgaa gcagacatac aaattgaaag tctacaagaa ctggaacaaa
     9781 ttgttttaga aaaaggttag tgaattaatg gaaataaggc tatatatctg tgctagctat
     9841 tttgactaac caaaaaaact tgctataatt caggtgtagt aaaaggtgct ggtaacacct
     9901 tttacgaaaa tgcttcggca gtgataatgg gattggcttt ttaagttttt tattttgttc
     9961 gcaatcccta aggtcatgtg aatgatccta gggattgcta gaatattttg caacagaacc
    10021 aaaatacgaa tagttatcaa gcagccatat tgactgcggt caatttagga ggggatactg
    10081 atactactgc tgccgtaact ggtgctcttg cgggtattct ttacgggggt gaagtgatcc
    10141 ctgaaaattg gagaaaatcg cttcaaaatg accagcttct taaaacgatc agtcagagat
    10201 tttgcgatac cgtgctatct caaagaattt gaataatgga tatgatgcaa atcttgtttg
    10261 tactaacgtt gtattgacaa aaaggatttt atttagtaca atgttagtac aaatgatgaa
    10321 cggaggtaga atcatgtctc aatcaaccat tacatttagg attcccgacg atgaaaaaga
    10381 actggtatta gaatatgcaa aagcacacaa ttcttcattg accgaacttt atcgaaatgc
    10441 tgtactggat aaaatcgaag atgaaatcga tctacggact ttacggagtg caattgaagt
    10501 ttccaaagag aaaaaagaga gtggtatttc tcaagaggaa atggagaaac tgctaaatga
    10561 attataaagt catctacatg ccgcaagccc agaagcaatt aaaaaaaatg gacagaaacc
    10621 aagctaaaat aattgtcgcc tggatcagaa agaatttaga aaacacgact gacccgcgtc
    10681 agcacggaaa aggtttgact ggaaataaga gtggggaatg gcgctatcga attggaaact
    10741 atcgaatatt ggctaatatt tgtgattctg aaattgaaat ccagatattc tcaattggac
    10801 atagaagtac aatatataaa agataaccgt ttttggaacg aagtagagtt tcgaaagtca
    10861 atgtataatc ttttgaattt tgcataatac cacaggtaga aactacccga tactatcggt
    10921 tggtttctgc tttttta
//
LOCUS       DACECR010000098        10662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.098, whole genome shotgun sequence.
ACCESSION   DACECR010000098 DACECR010000000
VERSION     DACECR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.098"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            334..2280
                     /locus_tag="IWO17_002317"
     CDS             334..2280
                     /locus_tag="IWO17_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0291175.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2283..2738
                     /locus_tag="IWO17_002318"
     CDS             2283..2738
                     /locus_tag="IWO17_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0291176.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2752..4080
                     /locus_tag="IWO17_002319"
     CDS             2752..4080
                     /locus_tag="IWO17_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0291177.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4114..4398
                     /locus_tag="IWO17_002320"
     CDS             4114..4398
                     /locus_tag="IWO17_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0291178.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4400..4915
                     /locus_tag="IWO17_002321"
     CDS             4400..4915
                     /locus_tag="IWO17_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ0291179.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4931..5593
                     /gene="rpe"
                     /locus_tag="IWO17_002322"
     CDS             4931..5593
                     /gene="rpe"
                     /locus_tag="IWO17_002322"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0291180.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5600..6172
                     /locus_tag="IWO17_002323"
     CDS             5600..6172
                     /locus_tag="IWO17_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0291181.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6359..7879)
                     /locus_tag="IWO17_002324"
     CDS             complement(6359..7879)
                     /locus_tag="IWO17_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0291182.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8122..8307)
                     /locus_tag="IWO17_002325"
     CDS             complement(8122..8307)
                     /locus_tag="IWO17_002325"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ0291183.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8291..8473)
                     /locus_tag="IWO17_002326"
     CDS             complement(8291..8473)
                     /locus_tag="IWO17_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291184.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8714..8932
                     /locus_tag="IWO17_002327"
                     /pseudo
     CDS             8714..8932
                     /locus_tag="IWO17_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9427..10026
                     /locus_tag="IWO17_002328"
     CDS             9427..10026
                     /locus_tag="IWO17_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0291185.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10090..>10662
                     /locus_tag="IWO17_002329"
     CDS             10090..>10662
                     /locus_tag="IWO17_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291186.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         3525 a         1674 c         1990 g         3473 t
ORIGIN      
        1 ttaggactca attgttcata gatgagcatt tgtaattaag ttatattata ataaaaaaat
       61 acgaatttta gtgacgccat tatattgttt ttatttttca attgtaatat ttcatattac
      121 ttccataata aggagtattt tctattggaa tatcttagct cttttaagga tttctccttt
      181 tttgtttctc attttttaag attatttgga tttcctttac aacaaacttc cacaagtatt
      241 attgacatag atgtaattga gggaaggggg ttccagtgct agaataaata atgaaaagag
      301 gtaacaaatg ctagcgttaa gaggtgaaca agtttgattg aaagacatgt aaatttatta
      361 aaattattaa gtcgacaaga agactttaaa cccgcttctt tttttagtga taaattggca
      421 gtgtcgacca agaccattta tcaagatatt gaattgttgg aagattatgt agttaattac
      481 caagtaacaa ttgagcgatt accttctttg ggtatcaaac tcataggtga taggaaaaac
      541 gttgaacatt tgatttcatc ggtagaaaat tatcttgaaa aagatattta ttctccagaa
      601 aaaagacgtg tcgagatcat taaagaagtt ttgtttggtg ataagcctgt ttctttaact
      661 catttgtctc aaagttttat ggttagtaaa acatcgttat acaatgacct gagaattatc
      721 aataaaatta ttggcagtgg agaagttaag ttgcagtcat ctgcagaagg aattagtttt
      781 actggtaatg aacaagcaat tcaacgagca atcaagcaac ttgtattcta ttatgcgaat
      841 gacatggaat caaaaacatt ttttcaagtt ttaaatatgt tgtttgattc aaaagttatt
      901 agtgtcattc atgatttgct attcaatgat tacagtgaac tgactgaaaa ggtttctgat
      961 tactatgttc ggtcattgat ttctacttta gttatccaat gtaaccgttt actgaaaggt
     1021 catcatttaa ctgttgaaga agaattcctc ttcaatagca ttcgctatat ggaaacgtac
     1081 attgtggcaa atggtttaat tgaagaattg aaggcaaaat tgaatcttcc attttatgac
     1141 gcagataaag aatatctatg tcgtcaacta tttgcacatc gtgtgacaaa taatttaaaa
     1201 accagcaatg aagagtacgc tatgagcgtt cataaattga ttgaaagaat gagtgagatt
     1261 gaaaaaattg atttgactca aaatgatcga ttatatcaat ccttattgta tcatgtgcca
     1321 gcaatggtcc ttcgtttaaa aaaaggtatt aaaattcaaa atccgttatt agaaaatatc
     1381 aaatctcaat attcagaact ctttagcata gtatggtacg ccatgtccat ccttgaagaa
     1441 gaatttgatg tgactttaac ggacgatgaa gtttccttaa ttttgattca ttttcagatt
     1501 gctattgaag atcagtccaa agcaaataat atcatcatta tttgccaata cggtatttct
     1561 tctgcgcaat taatttacaa taaagttcga cggtttttac cagcaaaaga taatgtagaa
     1621 atatcaactt tagataagtt tcatttagct gaaattgggg aagttgattt gattatttcc
     1681 tctctttcct ttgatgcagg tggtataccc ttagtaaggg tatctccatt ggtaagtaaa
     1741 acagattata tgaatattat ggaagcttat actaaatatt taatagaaga accttcgcga
     1801 tttattcaaa atgaactgat tcctaaagcg gttaaagttc ccaccatatc taaatttgtt
     1861 caatcatctt tagtaaaaac aggagtacag gtggaaagca aagaagaatg tttggatttg
     1921 atgatttccg aactagaagc caattcatta gttactgaag agtttcgtgc ttctatcttc
     1981 tctagagaga aaatggggaa tactaattta gaaagtggtg ttgctttacc gcatgcgaat
     2041 ccatcaatgg tcattgaatc caacattttt attgtagcat taaaaaatgc gattaattgg
     2101 ggcgatcgaa aagtcaaatt agtcattatg gttagtttag ctgaagaaga tgtatctgag
     2161 attagaggag tatatgaaga actttaccaa tttattgggc aaaaagaaat ggtcgatcgc
     2221 ttgttacaag ttcatgatag cgaaaaatta ctaagaattt ttcaacaaaa aggagaataa
     2281 aaatgtatct agataaagaa ttatgtttat ttcaattaga agtgaaagat caaaaagaat
     2341 tgtttcaagt aatgtctgaa caattaaaaa acgctggttg cgtgaaagat agtttcttag
     2401 aaggtatcac caaccgtgaa caagaatttc caactggatt agaagtaaat caaattgggt
     2461 ttgctatccc acatacagat tccgcacatg taaatagctc tcaaatctgt tttgcctcat
     2521 taaaggaacc actggtattt tcagatatga cagataaatc tcatgaaata cctgtacgtt
     2581 tagtctttat gttggcaatg tcacaacctc atgaacaaat tgatacacta caaaacttag
     2641 ttagcctatt tcaaaatgaa gaaaaagtaa atgaattact tgcatgtacc acgaaagaag
     2701 catttattga gttagtacat tctgcaggag tttattagga ggaatagaga gatggatatt
     2761 gtattaaatg cctttgaatg gtttattgga ttagggtcaa acgtcttttt accactgatt
     2821 atttttgtaa tcgggatatt atttggatta aaacctggta aagcatttgt ttccggcgtt
     2881 actgttggta tcggttctat tggtttagga ttagttttaa gtttattatc agatggttta
     2941 ggaacagcaa ttcaacaaat gggggaacaa tacggtggtg gcctgaatat cctagatatt
     3001 ggcgtaggtg ttggtggacc tttggcttac tctacatcat tgggaatttt aatgattcca
     3061 atttcattaa ttcttaactt catatttgtg gttttgggtt ggacaaaaac attgaatgtt
     3121 gatatttgga acttttggtt cgcaattttc ctaggtatga ttactcaatc tgttacggga
     3181 aacttttggt ttggcttaat tggcgcgatt gtcgcagtaa ttttacaatg gttcttagca
     3241 gatcgtacac aaaaaactgt tagcgaattt ttcggctatc caggaatctc tatcacacat
     3301 atgatgggat tatcaggagt actatttgcc aaaccagtaa attgggtttt tgatcgtatt
     3361 cctggattta ataaaataga tgcagatgca gaaacattaa caaaacgttt cggtgtttta
     3421 ggtgacacag ttgttattgg cctaattatc ggtattgtag tgggtattat ggcaggctat
     3481 gacgtagcag gaattggaaa cttaggaatt atgactgcgg cagtgatgaa gattatgcct
     3541 aaaatggtag caatgtttat ggaaggttta atgccaattg cagaagcggc gaaagaattt
     3601 acgaataaaa agttaaatgg tcgccaagta aacattggaa tggatgcagc gttaactgtg
     3661 ggtcatccaa ctattatgtc aacttctcta ttgcttgtac caatctcatt attgctagca
     3721 ctaattcttc caggaaataa agtattacct tttggagatt tagcattctt tgctttcgcc
     3781 atctgtttaa tgattcctta ttttaaagga aatattgtga gatcattagt aggatgttcg
     3841 ttgtatgttg ttacaacatt atatatggca acatggttgg caccaattat cactgatgtg
     3901 tttacattag caagtttcga tgtggggaca gcaggattag taactatgtt gttatgtgga
     3961 ttatggccag tagggttgtt tgttttatta gcatcttact ttggtgtaat tggaataggt
     4021 ggattaggag ttgttttagt aggtgcttct atctacatga acaaagtgaa gaaagcttaa
     4081 tcataaaatt aaaataagaa agaagagatc aatatgaaaa aattattagt tatttgtgga
     4141 gcaggtcatg ctacttctac aattgcagta gcaaaattat caaaatggtt ggaagaagaa
     4201 ggattaacag atcaagtgaa aatttatcaa tctaaaattg ctgatgagct aaataaaatt
     4261 gatgaatatg atgctgtagt aagtacaaca attgtccctg atgtaattaa agctaaagta
     4321 acgaatggct tgggcttatt aacaggtatt ggtacagatg ccatttatgc agatgtaaaa
     4381 gaaagattag gtgtttaata tgcgttttca tcatgacact ttgactattg aaacagtttc
     4441 agggcgtcct tcgtatcatg atattcgaaa agaactttta gatacagttg atacagcagg
     4501 tattgagaat ggaatgttag taatttcgag cccgcataca acttgctcac tcttttttga
     4561 agaaacgatg catgacacaa acttttttgg tgatgattat ttacatgtag acatcaacaa
     4621 tgtaatggaa acgattattc caaaaatgac gagtgaaaat caatacaaca gtccggggcc
     4681 aaaacacatt gattttggaa caagattatc tgatcccaat taccctgcag aaaaatgggt
     4741 catgctaaat accgatgcac atttacgttc atctatttat ggaaccaact caatgacttt
     4801 aattgttcaa gaaggtaaac ttttattagg agaaatgggc cgtgtctatt ttgtcgattg
     4861 ggatcaacta agagagcgaa ctagaacaat caatctcatg attatgggag aataaacggg
     4921 ggaatagaga atgacaattg ctccatcatt gttaaatgca cctatcgcag aagttaaaga
     4981 gttatttgaa atcttaaaag aaaagaatgt tgattatttg catattgatg taatggatgg
     5041 ctgttttgta ccagatatgg cttttggacc aagtgttatt aaagatattc gtccactaac
     5101 agacgttgtg tttgatgttc atttgatgat tgatacacca gaaaaaattg tcacaagatt
     5161 tgtagaagca ggagcagata ttgttacctt ccacattgaa gctaccaaac acgcaatgaa
     5221 ggtcattcag ttagtcaagg aagcaggagc aaaagtaggg attactttta atcctgcaac
     5281 accagtcagt tttattgaac cagtattatc tttagtcgat caagtattag taatgacaat
     5341 caatccagga acagagaata agcattttat tcaagagaca gtagttaaaa tcgaacagtt
     5401 agatgtaata cgcaaacaaa atgattatac gtatgatatt gaagtagatg gcaagattga
     5461 taatcaaacg attaaagttt gttctaaagc aggggcggat atttttgtat caggcggttt
     5521 catcttcaat gggaaaaata ccccgcaaga acaaattgat aaactaaatc aatcattaga
     5581 ggaagtgaag taaaagatca tgaccaataa agaccaaaat aaaaactatt tagaagcgat
     5641 tatcttagaa ttacaaaata actcaaaacg tattaattct gtagagttag aacaatttag
     5701 tgagaaaatt gttcaagcta aacacatctt tctagctggt gcaggacgct ctggaattgc
     5761 catgcaagca tttacgaatc gcttaatgca tttaggtttt tcagtcagcc ttgtcggtga
     5821 aattagttca ccacattcgc aacctggaga tttattaatt atctgttctg gctcaggtga
     5881 aacaggtagt ttaaaaaatt tagcggccaa agcaaatcaa agtggtgtaa gcattgcttt
     5941 agttaccatg aaaaaagact cgtcgattgg tcaattagca gattctgttc ttgtattacc
     6001 aggtattacc aaagatgaaa atcaacgaga agaagatact ttttcacaac ctatgggttc
     6061 aagttttgaa caattagcat ttctcacctt tgatagttta gtgttatctt taatggataa
     6121 aacaggtgaa agctcagaaa cgatgtttgc tcgacatgca gattttgagt agtagatttt
     6181 aaagataaaa aaccattctc taaatgctaa ttattcagca aatatgagaa tggttttttt
     6241 atttgattag acataactta ataattcagt agttttgagc gtaagcgtgt catagaatgg
     6301 tacctaaaca caaatagccc gatttgatac actcaaatca ggttatttgt ggcttatatt
     6361 atttacagat ttcttgtatc tgctgattcc atggcaaata agcgcgtagc gcttcaggtg
     6421 tcatcgaaga cagattggga atctctgtaa aaagatgatt taaatatttg cggatgttta
     6481 acccattcgc aacagctgtc tggataatac tcaatataat tccatttgat ctagcgcctt
     6541 ttaagctttt agaaaatagc cagttttttc gaccgatcac taactctttg atggcacgct
     6601 ctgctcggtt atttgaaagt tccaagcgtc catcaagcaa cacaatctga agtccgtctt
     6661 tatgattgac tgcgtattga accgcctttc caagctgcga ttttggcaaa taagcaaaac
     6721 tctcacacca atgaaaaaat tcagtcatca caggctgcaa cgtttccttc ctcgctacaa
     6781 acctttcttc tgcgctaagc ttattaaagg tccgttcgat tcgaaacatc ttgtcacaga
     6841 agtctaatcc tattttggca tgactttttg gggacggttg tttacccaat gcttcaaaaa
     6901 actttctgcg aacatgcgcc caacaataaa cattgtttac cggcagcttt ccataggccg
     6961 cgtaaccatc agtatgcaag aaaccgctaa atgattctaa aaaacttttt ggctcatcaa
     7021 cggaacgcga atttgcgtgc tcgtagagca caacttgttt ttcttcgctt ttacctgaag
     7081 catataacca gtaaaaggtc ttttctttat gagtattcaa tacccgatag cttgtctcat
     7141 ccgcgtgtaa gacctcctga ttgcttaatt cttgatgaag gcaattgtag attggctcta
     7201 aaaccatttc acttaccttg atatgccaat tagtgatttt tttacgatca agagggaaac
     7261 caagtgcatg ccattctttt tcttgtcgat atgccggcac ctttttaaca aatttttccc
     7321 aaatactctg tgtcacaatt gaagaggaac ctagactatt attgatcaac ggtttaggaa
     7381 caggcgcttt tttgattttt gtttctccta atgcttcaca ttctctgcaa tgataggcat
     7441 gttggtagat gacttgtttt tctatatggg caggaacata caatggctct tctctaagcc
     7501 ttgtcgcacc gatatcatgt aaagtatgac cacagtcttc acacaaacaa tcggcacctt
     7561 ttaattcata atgaatagga acttcaggca gaccttctaa tgaaagtgct ttgagtccct
     7621 tgcgttttct gcgtttaaaa gaagataccg taatgggttc ctggctttct tgtccagtgt
     7681 gctctggttc attaaaagtt gagtcgtcta ataaatcgat ttgcccatct acgggtggtg
     7741 tttctttttt tcgtccaaac aagcgtcgat tcatttcagc aatttgttct ctaagaagag
     7801 tattttcctg ttgctggatt tcaagcaact gtgttaattg cttgatttgt acaagaagtt
     7861 cagattccat ttttgtcatt tttacaccac cattctagcg attttcttac atctattata
     7921 ccattttatg acaacgaaaa acagacaaaa tccttgattt aacaaggttt tcgtctaata
     7981 tatcgcacct tttttcgttt cttttagcgt acatttttct tcgattgaaa ggccagtcag
     8041 taagacttct tttctggaac gcggccattg aaggtaccca ctttcaaatc ttttgtacat
     8101 taatacaaat ccattttgat cctagtaaag agccttgaag cgatctttct tcgtaccgca
     8161 aaataagaaa agcgcttgat cgtaaacatc aaggtcatat tcttctataa cgaaagctgc
     8221 tagtccatca attccttttc gaagatctgt ttttccacaa acgataaaaa tatgtttcaa
     8281 tgcagttgca tcaatgagca ttggcattca actcttttaa caaggtatac aaaagatatt
     8341 tatccattcc attataaata aatatttcct tatcttccga tttgattctg caagctagtt
     8401 ttggcttggt gtcaatttgc gatttttgag ccgcacttga aaattgaact ggtacaatat
     8461 cttttttatt cattcgtttt tctccttcta tatttgatag tttaattata gaagaaagat
     8521 aatcggcgta cgagatacta tcctatgaca cgcttacatt taatttaata ttttgcctca
     8581 aattagccac actttttata gaactaagag gaaaaatgaa gttttcttcc tattaatata
     8641 gttgtttgaa atcttaaata tagcgtttta cgatgtaatg ggaagtatgg tgaataaatc
     8701 aactatgttc gtacgaagca tttactcaac taatttaatc gaatctttca ataaacaaat
     8761 caagaaatac agccaccgca aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt
     8821 agtctcgtct tttgatactt acaaccaaaa attcctaggt cgcagtcata aaggtttcca
     8881 acaggccgaa ggcgagctcg aacaaatgct gagtcaaccg atggagaatt agaaacaatc
     8941 tacagtaggg aagaaccatt gacacaaaat tattgacgct cccaactaaa agtgtcaaaa
     9001 taatgatgaa atcatttagt tcttataaat tagaaaagag aaaataaata ttccatatct
     9061 actttattgg atccgtaaca tgactaaatt ctgaatactt tttaaagctt atcatttggt
     9121 aaaactgaat ataagttaat tatatagact gaaaattttg aattttcaga atagaataac
     9181 ttaaaagtac tgacaaaatt ttttaaaggg gcaatattaa tgttctgaga taatcaaatc
     9241 ctaccgaaaa tatcaaaaat ccaatgtgac acaatcagat tgtgtctcat tcgagggaat
     9301 cacacaatcc cacgtggctc taggcagaat tgaatttttg cttggggctt tttccttata
     9361 ttgattttga atgagactta ttatatgtta taatgcaatt aagtatcatt ggagagggcg
     9421 aagaaaatga agacggtaga acctattcga gacaaaaaaa agatcgatgc gatgaaggca
     9481 atcttggctt cgggaaaata tggccaacga aatttggttc ttttttcgat tgggatcaac
     9541 accgcctatc gcatttctga tttgcgtcaa ttgaagctgg ccgacgtgct ggaaatttca
     9601 cgcggtcgcg tggtggtcaa agatcgatta gcgatgaagg aacaaaaaac agctaaacat
     9661 aattcggtct atatttccaa caaattgcga aaaacgattt tggagtacgt tcaacaggaa
     9721 tttcctgatc aactccttca gcaagatttt gacggttatt tgttccctag tcgaaaaggg
     9781 acgaatgaac cgctcaaccg ccaaagtctg tggcgaatca ttcatgatgc tggcacggca
     9841 atcggattga aagacattgg cccacattcc atgcgaaaaa cgttcggtta ctttctctat
     9901 aaacagggga ccaaaacaga aatcattcag tccttgttga accattcttc tcagcgcgaa
     9961 acattgcggt atattggtat tacacaagaa gacaaagata cagctgtaaa aagcttagat
    10021 ttataatata aaagttaagt ttaaacatgg atagttttaa attagtagat tgagtgttgg
    10081 gaggaaagaa tgagtacatt atatattata ggaaacggtt ttgatttaag tcatggttta
    10141 aaaactagat acactgattt ttcaaaatgg gttgaaaaaa gtcatagtga aatattcgat
    10201 aaaatagaca caatgataaa tgaaaataat gaggtgagaa gtacagctga tgataagata
    10261 ggttggttta attttgaaag tagtttgcat gagttaattc aaagtccagt ctttgaagat
    10321 tatgtagaag agtctaagga ttatctcata ggttatggac atgaagattg gtcagattct
    10381 gcccatcatg attttcaaaa tatggtttat gattatgttg tttctctaga agaattacaa
    10441 caacttttca aggaatggat acgcaaagaa atacagccga caatagaaca taaaattcca
    10501 aaataccaat tttcgtccga tgtactattc tttacattca attatacaga tgttttggaa
    10561 attctataca agcaaccgaa agaaaacata tatcatattc atggcagtta tgacgatata
    10621 ataatcggcc actccgagaa ttttgacaac tttataggag at
//
LOCUS       DACECR010000099        10370 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.099, whole genome shotgun sequence.
ACCESSION   DACECR010000099 DACECR010000000
VERSION     DACECR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.099"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            282..521
                     /locus_tag="IWO17_002330"
     CDS             282..521
                     /locus_tag="IWO17_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291187.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="IWO17_002331"
     CDS             complement(883..1143)
                     /locus_tag="IWO17_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291188.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="IWO17_002332"
     CDS             complement(1328..1825)
                     /locus_tag="IWO17_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ0291189.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IWO17_002333"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IWO17_002333"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ0291190.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IWO17_002334"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IWO17_002334"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ0291191.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..5209)
                     /locus_tag="IWO17_002335"
     CDS             complement(4547..5209)
                     /locus_tag="IWO17_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0291192.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5219..6034)
                     /locus_tag="IWO17_002336"
     CDS             complement(5219..6034)
                     /locus_tag="IWO17_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0291193.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6296..6739)
                     /locus_tag="IWO17_002337"
     CDS             complement(6296..6739)
                     /locus_tag="IWO17_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ0291194.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6873..7211)
                     /locus_tag="IWO17_002338"
     CDS             complement(6873..7211)
                     /locus_tag="IWO17_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0291195.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7199..7576)
                     /locus_tag="IWO17_002339"
     CDS             complement(7199..7576)
                     /locus_tag="IWO17_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0291196.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7800..8993
                     /locus_tag="IWO17_002340"
     CDS             7800..8993
                     /locus_tag="IWO17_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0291197.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9159..9581)
                     /locus_tag="IWO17_002341"
     CDS             complement(9159..9581)
                     /locus_tag="IWO17_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0291198.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9976..10170
                     /locus_tag="IWO17_002342"
     CDS             9976..10170
                     /locus_tag="IWO17_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291199.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            10167..>10370
                     /locus_tag="IWO17_002343"
     CDS             10167..>10370
                     /locus_tag="IWO17_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291200.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQA"
BASE COUNT         3166 a         2049 c         1710 g         3445 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
     5041 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
     5101 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
     5161 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
     5221 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
     5281 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
     5341 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
     5401 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
     5461 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
     5521 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca
     5581 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg
     5641 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
     5701 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
     5761 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
     5821 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
     5881 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
     5941 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
     6001 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
     6061 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
     6121 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat
     6181 ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc
     6241 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     6301 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     6361 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     6421 ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     6481 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     6541 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     6601 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     6661 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     6721 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     6781 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     6841 tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc
     6901 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     6961 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     7021 cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg
     7081 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     7141 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     7201 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     7261 gtaaacgttc caaacactct tgcatttcag caatttcttt cgtcaaaagt tcactttctt
     7321 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     7381 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     7441 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     7501 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     7561 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     7621 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     7681 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     7741 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     7801 tgacaacgta tcagcgaaag gtattattat cgacatcggc gggaattgct ttagagaata
     7861 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     7921 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     7981 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     8041 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     8101 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
     8161 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     8221 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     8281 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     8341 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     8401 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     8461 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
     8521 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     8581 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     8641 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     8701 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     8761 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     8821 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     8881 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     8941 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     9001 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     9061 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     9121 gctttttcaa ctgctaattt ccttctatct ggcggcgact attctttgac gtccgtcatt
     9181 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     9241 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     9301 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     9361 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     9421 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     9481 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     9541 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     9601 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc
     9661 aatagtgcat aaacctgtta ttatttctct ttttttccta agtattctat tgatatgaat
     9721 catttaatcg tttattatat aaatgtcaat aatctaataa taaaataacg tacatatttt
     9781 attattgcat acttaataac agaactatag caaaaaatcc cgtgtatcaa cacgggatta
     9841 gattaagttt cctcaatcac tattcataac acaattatta taacaagtaa gtaaacaatt
     9901 gaaaaggaca tttattgaaa acggttgttt tttacttaag gaggaaattt agcgaaaaaa
     9961 ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg ggtgtggtgc tactacgtct
    10021 actactcaaa gcacaagttc agatcaggct tccagcagca gtaccacgat ctccgaaagt
    10081 acggcagatt catcgaaaga acaagaacaa attgaaaaga aaaaagaaga gaaacaaaaa
    10141 gcggaaaaag ctgcactaga aaaaaagtga cagaagcgga atctgctttg tcacaagctg
    10201 aagcaaatcc tacaaaagaa aactatgcaa ctgcttcagc agctatccaa gcgctacctg
    10261 aaaacaaaca agagttgaca agccgtcttg ctgcaattga ttccacaatc aaagcaaaag
    10321 aagcagaaga agaacggaaa aagcaagaag aaattgcacg acaacaagca
//
LOCUS       DACECR010000100        10085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.100, whole genome shotgun sequence.
ACCESSION   DACECR010000100 DACECR010000000
VERSION     DACECR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.100"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWO17_002344"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWO17_002344"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ0291201.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IWO17_002345"
     CDS             complement(1172..1867)
                     /locus_tag="IWO17_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ0291202.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IWO17_002346"
     CDS             complement(1880..2596)
                     /locus_tag="IWO17_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ0291203.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IWO17_002347"
     CDS             2926..3534
                     /locus_tag="IWO17_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ0291204.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IWO17_002348"
     CDS             3859..4182
                     /locus_tag="IWO17_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0291205.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IWO17_002349"
     CDS             4364..5734
                     /locus_tag="IWO17_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ0291206.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IWO17_002350"
     CDS             complement(5864..7165)
                     /locus_tag="IWO17_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0291207.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IWO17_002351"
     CDS             complement(7519..9510)
                     /locus_tag="IWO17_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ0291208.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IWO17_002352"
     CDS             complement(9654..9920)
                     /locus_tag="IWO17_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0291209.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
BASE COUNT         2955 a         2094 c         1829 g         3207 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta acccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgt
//
LOCUS       DACECR010000101         9614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.101, whole genome shotgun sequence.
ACCESSION   DACECR010000101 DACECR010000000
VERSION     DACECR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.101"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            154..2421
                     /gene="gshAB"
                     /locus_tag="IWO17_002353"
     CDS             154..2421
                     /gene="gshAB"
                     /locus_tag="IWO17_002353"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ0291210.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(2510..2962)
                     /locus_tag="IWO17_002354"
     CDS             complement(2510..2962)
                     /locus_tag="IWO17_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ0291211.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            3112..4062
                     /locus_tag="IWO17_002355"
     CDS             3112..4062
                     /locus_tag="IWO17_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0291212.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4055..5014
                     /locus_tag="IWO17_002356"
     CDS             4055..5014
                     /locus_tag="IWO17_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0291213.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            5011..5766
                     /locus_tag="IWO17_002357"
     CDS             5011..5766
                     /locus_tag="IWO17_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0291214.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            5789..6742
                     /locus_tag="IWO17_002358"
     CDS             5789..6742
                     /locus_tag="IWO17_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0291215.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(7386..7682)
                     /locus_tag="IWO17_002359"
     CDS             complement(7386..7682)
                     /locus_tag="IWO17_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ0291216.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            8037..8393
                     /locus_tag="IWO17_002360"
     CDS             8037..8393
                     /locus_tag="IWO17_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0291217.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            8403..9302
                     /locus_tag="IWO17_002361"
     CDS             8403..9302
                     /locus_tag="IWO17_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ0291218.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         3003 a         1639 c         1956 g         3016 t
ORIGIN      
        1 ttgcagctcc ttgtttgaat gattgttttt aacatagctg tgtttttgat gccttcattt
       61 ttatacttat ttgtaagcgg ttctcaaaca atttcgttct ttttcttaga ctttttgatt
      121 ataattagat tcatatgaat tggaggtcac ttaatgatga attttaagca attattattg
      181 catgtcaatg cgcgcccttt tatcgaccaa gctcgatttg gtatagaacg ggaaggacag
      241 cgggttgatc ttgcaggaaa tctagcaaaa accgatcatc cagcaatctt tggcgatcga
      301 tcctatcacc cctatatcca aacagatttt agcgaaacac aaacagagat gatcacccct
      361 gttaccgatt ctattcccga attatttcag tatctcgctg ctgtttatga tgtgactgct
      421 cgttctatac cgaaagaaga gatgatctgg ccattaagta tgccacctgc cttaccggaa
      481 aaagacgaag agatcattat tgcaaaatta aaaaatttcg aagatgtctt gtatcgacgt
      541 tatttagcaa aagaatacgg gaagcgaaaa cagatggtaa gcggtattca tttcaatttt
      601 gaatttggcg atgagttgct aagaacattg ttcagccatc aggaagaatt tcaagatttt
      661 tccgaattca aaacagaact ttatttgaaa acagcgagaa actttatgcg ctatcgctgg
      721 atgatcactt atttattcgg tgcttcacca atgagtgaga agaactactt tttagatgaa
      781 tcacatccgc aagaacctgt tcgcagtatt cgcaacagtg cgctgggcta tacgaatcat
      841 ccaaatgtga aagtttccta tgcttctatg aaacagtatt tagcagatat cgagcgaatg
      901 atcgaagaag ggaaactttc ggaggagaaa gaattttata caccgcttcg tttccgaggc
      961 ggaaagaaag tcgcagattt agcaacaaca ggtgttcgtt atattgaatt gcggaatatc
     1021 gatttgaatc cttatgcaag attgggtatc aatccagagc aagttcggtt cttacaatta
     1081 ttcctgatgt acatgttatg gacagaagaa aaagaagact gtgaccaatg ggtagcagaa
     1141 ggtacgactc gaaataacaa agtagcacta gaacaaccat cagatcaaac agaatttcat
     1201 caagaaggta gagaaattct tgaaggaatg aagcaaatgc tggttgaatt ggattggttg
     1261 gattctcttt atttagtaga ggaagcattg actcaaatgg atcatcctga acaaacgtta
     1321 gcagcgaaac tctatcaaga agcacagcta tcgagtcagc aagaagttgc tgtggcattg
     1381 ggacatcaat attataaaga aagccatgaa cgtccctatc agttggccgg ttttcgcgaa
     1441 atggaacttt ctactcagat atttatgttc gatgccatcc aaaaaggtgt ccaagtcaaa
     1501 gtattagatg agtcggatca atttttgcgt ctgcaattcc aagaccatgt agaatatgtg
     1561 aaaaatgcaa acatgacaag taaggacagc tatatcgtcc cacttatcat ggaaaacaaa
     1621 acagtaacga aaaaagtctt aaaagaagct gggttccggg taccaggagg cgcagaattt
     1681 tcatccatgg aagaagcggt aaaagcttat ccgagatttg ccgagcaggc atttgtgatc
     1741 aaacctaaat caaccaatta cgggttaggt atcacgattt tcaaggaagg agctagtctg
     1801 gaagattatc aagcaggttt agccattgct tttcgtgaag acagttcggt tttagtagaa
     1861 gaatttatgc cgggaacgga atatcgtttc tttgtgatcg acggagaagt ccaagccatc
     1921 atgttgcgag tccctgctaa tgttattggt gacagtatcc gaacagtgaa agaacttgtt
     1981 gaagaaaaaa atagtgaccc tttgcgtgga acgaatcatc gtgcaccttt agaattgatc
     2041 caattaggtg aattagaaca attgatgttg aaagaacaag ggttgacgat cgaatcagtt
     2101 ccccaagcca atcaaattgt ttatctaagg gagaactcaa atatcagtac aggtggtgat
     2161 tcgatcgata tgactgatga attttctgaa gcttacaaaa aaatcgccgt ttctgcggta
     2221 gaagcactag gagctaagat tagtggtatc gatttgatta taccagataa agaaatcgat
     2281 cctacaactg ataaaaaagc atatgggatc atcgaagcaa atttcaatcc agccatgcat
     2341 atgcatgttt atccttttgc aggaaaaggc agaagattga cgatgaatgt attgaaattg
     2401 ttatatcctg aagtatttta aaagaaaaag cttttgacag attgtcctat ttgatgtcaa
     2461 tctgatcaaa agcttttttt gcatgtaaaa atttatgtgt tttttcgttt taaaataaac
     2521 gatcccagaa gctttttttc ttttttggtt tttctttttc agctggcgaa gagttttctt
     2581 ctgggtattt tttttcgata tcaatcacag gttcatttac tgcttctttt gctgttaaga
     2641 gcaatccgaa ttcctcagga tttgcagtga cgtaatcgtt gactacagtg aaacggctat
     2701 tgtttttcgt tgctagctgg atatattggt tttggacaga aaggggtaga gccccattca
     2761 agagtatgga agcatctgga tgttttttga gttcttttaa aaagttttct ttgttttttg
     2821 gttggatcat ttcagcaaca gtcatactaa gatagcagcg ttccctgaat gtccctaaat
     2881 atttatgctg ttcttcaggg ttcactaaag gtgtaccata aagtccctta tccaaatggc
     2941 tttttacatc atcttgtgcc ataaaaaata ccctccttct tctctttgtc tttcttacga
     3001 ttatagcata ttcccaataa tacctaaaac atatgaaaaa gcggttgctt ttgttcaacg
     3061 attcacttaa aataagaatg aaaatcattc tcaattagaa aggtggattg gatgcgctac
     3121 ggacgactag tagcattatt agttatttta agtagtattt cattgttcat cggagtatcg
     3181 aatgtacaat tgtcagaact gctccagtta gatgggcaaa gcttacaaat cttgctagtg
     3241 actcgtttgc cacgaacgat ttgtttgatc cttgtgggag cgaccagtag tatttgtggg
     3301 ttgatcatgc aacatttgac ccaaaataaa tttgtttcac caacgactgc gggcacgatg
     3361 gatagtgcgc gtttaggtat tttagtggct atgattttcc tacctggtgc atccttgatt
     3421 gtccgatcct tgacagcatt ttgctttgct tttgcaggaa cattgctttt tttatccctt
     3481 acgcgattgt tcctccagaa aaatcaattg atgcttccgt tgattggagt catgttaggg
     3541 aatatcattg gttcagttgc tacttttttt gcttatcaat tccagttggt acaaaatatg
     3601 acttcatggc ttcaagggaa ttttgcgact gtgatacgag gaaattatga gttattgtat
     3661 gctgttgttc cattgttgct cttggtttgg ctatttgctt accgatttac gatcattggt
     3721 ctgggcgaat cgtttgcagt aaatctggga atagattacc agaagatgca gtttttaggc
     3781 attggcttag tatcattggc tagtgcagtc atgttgatca tggtaggaag cattcctttt
     3841 atcggagtga tcattccgaa tcttgtctct agaatatatg gagatcaagt gcacaaaaca
     3901 attggcgtaa ctgctttagc aggtagtttg ttcttagtat tttgcgatct tgtcgctcgc
     3961 gtgttgattt ttccttatga aataccagtc agtgtgatcg ttggaatcat cggtggggct
     4021 atcttccttt atcttctgat aaggggacga cgatatgctt aaaaaaaagc catctatttt
     4081 tattggtatg cttgttcttg ttttatttgt tacaggaatg tacttgacga tccatacata
     4141 tggaaactgg cattttgcat gtgcattgag gagtaagaaa atttttgcat ttatccttgt
     4201 ctcgctagct gtcagcagtg ccacaatcag ctttcagaca attacgcata accagttttt
     4261 gactccttca gtattgggac ttgagtcgtt atatgtcctt gtccaaacgg tccttttctt
     4321 ctttatcgga ggagtgacga tgcttcagca gacaggtaca gtttttttct tattgaacgt
     4381 tgggctgatg gtcgggctga gtctattgtt atttttcttt cttttgaaaa aaatgagagc
     4441 gaatcttttt ttattactga tgtcagggat gattttaggt acgctttttg gtagtgtcag
     4501 taccttttta caagtcatca tggatccgaa tgaatatgat ttgcttcaag gcagattatt
     4561 tgcaagcttt ggcaatatcc agagtcagta tcttttgcct gcttctttga tcattatcgg
     4621 ctgctggtac ctgttatttc gatatcgaca tgtgctagac gttttccatc tcggaaatga
     4681 acaagcagta aacctcggga tagacacaga tcgttttgaa tttttgattt tatgttgtgt
     4741 cagtcttttg acagggacat caacggctct tgtgggtcca gttacttttt tagggtttat
     4801 tgtcgcaaat atcagttatc gtgtcttttc tacttatcgt catggtcagc tatttatcgg
     4861 aagctttctg gtagctatgc tgtttctgac aggaggacag ctcgcagtag agcagatttt
     4921 cggctggaat acaacagtga gtgtcgtcgt tgaatttgtc ggaggaattt attttgtcac
     4981 aaaattgatt cgcgaaagga agagtatcct atgattgatt tacaaaaggt caataaactg
     5041 taccatggaa aatcagtggt taaagatgta cagttaacga ttcctgaaaa gaagttgact
     5101 gcttttatcg gtccaaatgg tgctggaaag tcaacggtgt tgtcgatgat cagtcgtttg
     5161 ataccaaaag atacaggtga aatctattta gaccataacg aagtaaaggc ttggaaatcc
     5221 aatgaattag ccaaaaaatt atcgatccta cgacaatcga atgaaataaa tgtccaaatc
     5281 accgtgaaag aattagtaga atttggacga tttccttata cgaaaggaaa attgacgaca
     5341 aaagacgagg aaatcgttca ggaatcactg gaacacattg ggttagaaga aatagcggat
     5401 caatcgattg acactctttc aggcggtcaa ctccaacgtg cgtatattgc aatggtgcta
     5461 gcgcaagaca cggattatat tttactggat gaacctttga acaatctaga tatgaatttt
     5521 gcggttcaaa tcatgcagat tttaaaaaat ttagtacaag aaatcgggaa aacaattgtt
     5581 attgttcttc atgatatcaa ctttgctgca agttatgcag acgaaattgt tgcaatgaaa
     5641 gatggacagt tatttacgca aggaacgacc caagaaatga taaaaaaaga tgtattggat
     5701 gaattatacg aaatggacat acggatttgt gagattgaag gccgcagatt ctgtctttat
     5761 ttctaaaact aacattagga gagaagagat gaaaaaatta gtagtaggta tcttgattct
     5821 agcatcattt ggtttagcag catgtggaaa tagcaacaac accagtcagg cagacacaaa
     5881 aaaaagcagc acacaaactg aaactacatt gacgataaca gatagtaatg gagaccaaat
     5941 cgaggtgcca aataatccag aaaaagtcgt cgtattcgac aatggttctt tagatacgat
     6001 ggatgctctt ggtgttggag ataaggtagt tggagcagct acaagcagcc ttcctgagta
     6061 tctttcatcc tataaaaaag tagaatcagc aggtggaata aaagaaccag acttagaaaa
     6121 aattaatcag cttcagccag atctaatcat catttctggc cgacaaagag attttcaaaa
     6181 agatctaagt caaattgctc cgaccatttt cctttcttta gatgcgaaaa atccatggga
     6241 atctttccag caaaatgtca ctgctttagg ggagatcttt ggtaagcaag aagaagctaa
     6301 aactcaatta gaggaattat ctagtgcaat cgatcagacg aaaaagaaag cagaagctac
     6361 tgacaaaaaa gcactagtca cacttgtgaa tgaagggcaa ttatcagcct acggatctgg
     6421 ttctagattc ggtttcatcc atgatttatt tggctttgaa caagcagatg atcaaatcga
     6481 agcatccact cacggtcaaa gtgtttctta tgaatatgtg ctggaaaaaa atccggatat
     6541 cttatttgta gtagacagaa caaaagctat tggtggagac gacagcaaag atgatatttc
     6601 tgccaatgaa ctggtagcac aaacgaatgc tggtaaaaat cagcagatca tctcccttga
     6661 accagatgtc tggtatttaa gcgggggcgg tttagaatca atgaaactga tgatcgaaga
     6721 tgtgaatcaa gcatttaaat agcataaata acagaggatt aaactctcat actatgtaac
     6781 aataatgttt cttacattct taaatagcaa gagcctatcc tcaaagaagt tatcaaaaac
     6841 catccgtaaa atcgtattga tgattttacg gatggttttt atttatttga gaaatctttt
     6901 ttcaacgtga attcattatc tacatcatac gggttgattc tagaatgggg agtggcaaaa
     6961 aatagtgtga tccatttttt cagcatcaag atcgcctcct ttgatttgaa tcaaaggtac
     7021 aaagcgaaac ttagataaaa cttaaattta gatattttta ttttgtaccg atctcttctt
     7081 taaacgatgt tccatctgct gcttctcggc aataagtcca ctagactaga aaatatgaga
     7141 agctattttc tgtccagcgt tcttattact caaaacaaat cgcagttgtc acaatctctt
     7201 ctaaacgatg ttccatctgc tgcttctcgg caataagccc actagactag aaaatatgag
     7261 aagctatttt ctgtccagcg ttcttattac tcaaagcaaa tcgcagttgt cacaatctct
     7321 tctaaacaat gttcaatcag ctgaccttcg gtattagctc aaagcactga tttcacaacc
     7381 tctatttagg catgtgtctc agcaagcggt tgatagttga tgatctgcat ttttcgataa
     7441 aaatcggcgg gaagcttact ccaccgtttt agtaattgat agaaataact ctcttctgct
     7501 ttgattttat actcgcttaa tatcaatcca aaactattcg ttttttttga agctaatttt
     7561 ttataaatat ccaatgcttt ggttttatct tcattgacaa agagtgtttt tacttcaata
     7621 taagaaccct cgatattttg tgtttcttcg ataaaataga tgttttttat atattccttc
     7681 atagtgattc ctccaaagaa gtcttttatt tgataagttt actctaccga agagttgtta
     7741 ataatccgtg aacaaaaagt aattcttttt ccaatagttg ttaaaggttt tttatttttt
     7801 atagtatttt ttgttattta agaaaggaat aaggttaaac aaataacagg ttgctttttg
     7861 ggtctttttc cgttcaagaa actgaacgaa gaaaagggaa agttgtctct tttgtgataa
     7921 gagaaatcat aataaacgaa tgacttactt tccaataaat tgagaacagt cagaaccaga
     7981 caaattagtt gcatctgatg aataaagtga ttattctatg acatgaggtg atacagatga
     8041 aattagaagt gacaaaagaa gcacaagaag tattgaagaa taaattagaa gcagacgatc
     8101 aacttttatt agatatcgaa aacggtgacg gtccatttgc agaaagccaa gtcagttgcc
     8161 aattggatac ggcatttcga ttgatcattg tcaaaaaaga tacacaaaca gatctaagtc
     8221 tttattctgt agtggctgat acaccagttg gaccaatcaa aatcaaagac agtgcaatac
     8281 tttacatgga tgatccatcc acacttgctt tagaaccaac ttataatagt ttacaattaa
     8341 aaggaccaag cggcttgaga aaaggcaact tgcaagttgt tcgaaaagac tagagggata
     8401 aaatgggaaa attggcgatc atcagcgacc tacatgtgga catcaataaa ttcggcgaat
     8461 cagagctgat gcagctttgg caagttttgc aaaagcacaa gatcactcgg cttcatctag
     8521 ccggagatac ggctaataaa gtagaccgtt gcctagctgt tgtcgaattt ttcgtcaata
     8581 aaggattatc tacgacattt catttcggca atcacgagtt ggcagatgtg cccaatgaac
     8641 agatgatgga ccattttcca gacccacatt ttttaaatga acggtatatt gctttaaatg
     8701 agcagaccgt tcttttaggg atgaatggct ggtacgacta tcagttttcc gaattgaaag
     8761 attacgaggc aatcgtacga atgaagcgtt tgtattggta tgatcggttc atccagcgtg
     8821 ggaaaacaga tctcgaggtc aatgcagaaa tgcttgcagc tgcaaaaaat ttgctagacc
     8881 agctggaagc aaaaaaactt gaggtgattt tatccactca tttcgttcct aaacgggcat
     8941 ttatcatgta tcaatctgct ccttatgagc gttggaacaa attaaatgca tttttaggat
     9001 cagaatcatt cggagaatta cttgaccgtt attcaaatat caaacaagta gtttttggcc
     9061 atacgcatcg tcgctttgaa gataaatcca tcggtcacac gatttacagc tgcagacctt
     9121 ttggttatta ttatgaatgg cagctgaccc gagattttgt cttgaaagaa cagttgctag
     9181 acagctatga tcctatgaaa ttacgtgtcc tcttgcgtgc ccatcaccca gcttttcagg
     9241 cctatcaaac acaacatttg caagaggaat ttgaacaagc gatgacaatc gttccgtatt
     9301 gagacagcat tagtaatgtg taaaagaaaa taccttgtaa aacataagga taagtaagag
     9361 cagtagatcc atttatcgtg gatatgctgc tttttttgcg gaaaataatc agcgaatgtt
     9421 tatttgatcg gatgatttgt aactcgcaga gtactagatg caagcgtgta ttcttttttt
     9481 gaacgataat cacaaaaaaa aatataatat acatttttta tttaattata attaatattg
     9541 attgaagtct aggcagtcaa gtcgagagtg taaaatattt tgtgtaaatg aaaaaatcca
     9601 tacaaaaaag gaag
//
LOCUS       DACECR010000102         9071 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.102, whole genome shotgun sequence.
ACCESSION   DACECR010000102 DACECR010000000
VERSION     DACECR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.102"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..528
                     /locus_tag="IWO17_002362"
     CDS             <1..528
                     /locus_tag="IWO17_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="similar to full-length Gnd, these proteins seems to
                     have a truncated C-terminal 6PGD domainin; in
                     Methylobacillus flagellatus this gene is essential for
                     NAD+-dependent oxidation of 6-phosphogluconate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase
                     (decarboxylating)"
                     /protein_id="HAQ0291219.1"
                     /translation="GARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGASGSGHF
                     LKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEIMEDQLN
                     EHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFSAKVVSA
                     LRNGFGGHEIVEKGE"
     gene            530..1531
                     /locus_tag="IWO17_002363"
     CDS             530..1531
                     /locus_tag="IWO17_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0291220.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1528..1812
                     /locus_tag="IWO17_002364"
     CDS             1528..1812
                     /locus_tag="IWO17_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0291221.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            1845..2642
                     /locus_tag="IWO17_002365"
     CDS             1845..2642
                     /locus_tag="IWO17_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0291222.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            2656..3489
                     /locus_tag="IWO17_002366"
     CDS             2656..3489
                     /locus_tag="IWO17_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0291223.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3507..4364
                     /locus_tag="IWO17_002367"
     CDS             3507..4364
                     /locus_tag="IWO17_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0291224.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4386..4895
                     /locus_tag="IWO17_002368"
     CDS             4386..4895
                     /locus_tag="IWO17_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0291225.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            4932..5333
                     /locus_tag="IWO17_002369"
     CDS             4932..5333
                     /locus_tag="IWO17_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291226.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5445..5798
                     /locus_tag="IWO17_002370"
     CDS             5445..5798
                     /locus_tag="IWO17_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291227.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            5832..6275
                     /locus_tag="IWO17_002371"
     CDS             5832..6275
                     /locus_tag="IWO17_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0291228.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6292..7377
                     /gene="uxuA"
                     /locus_tag="IWO17_002372"
     CDS             6292..7377
                     /gene="uxuA"
                     /locus_tag="IWO17_002372"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0291229.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7370..8083
                     /locus_tag="IWO17_002373"
     CDS             7370..8083
                     /locus_tag="IWO17_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0291230.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            8835..9035
                     /locus_tag="IWO17_002374"
     CDS             8835..9035
                     /locus_tag="IWO17_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0291231.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3039 a         1431 c         2017 g         2584 t
ORIGIN      
        1 ggagcacgac atcaagcctg cttaatgatt ggaggagata aagaaacttt tctatccgtg
       61 gaacaattat tcaaagatgt agcaattgaa agaggatatc tgtatgctgg tgcatccgga
      121 agcggacact tcttgaaaat gatccataat ggaatcgaat atggaatgat gcaagcaatc
      181 ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt ttgacttgga acaagtcgca
      241 cgtgtttgga atcatggttc ggtgattcgc ggctggctga tggaaatcat ggaagatcag
      301 ttaaacgaac atcccaattt aagtaattat cggggaatcg tttctgcatc aggtgaagca
      361 aaatggacgg ttgaaacagc gctagaaatg gctgttccag ttccagtaat tgcaactagt
      421 ttgttcatga ggaatctttc acaggaagaa gatagttttt cagcaaaagt agtttcagca
      481 ctaagaaatg ggtttggcgg acatgaaata gttgaaaagg gtgagtaaaa tgccagatta
      541 tcatatagca atcgttaatt ccagcagttt cggaagagtc tttccacacc acttggaaag
      601 attaaagtca gttggagacg ttcatttctt tactgttgat caagagattc caggaaaaga
      661 actagcggag ttacttaaag gattcaatat cattattgca agtgttacgc cattttttcg
      721 acgagatttt tttgaaaaca aagatgaact gctattgatt tcccgacatg gtattggtta
      781 caacaatatt gatttaaaag cagcaaaaga acaccagaca atcgtatcta tcatacctgc
      841 tttagttgaa cgaaatgctg tagcagaaaa taatataact aatttgttgt ccatgcttcg
      901 acgcacgaca caagcacaag caagagtcaa ggacgatcga tgggaagatc gggcggagtt
      961 cgtcggtagg acgctgttta acaaaacagt tggtgtgatt ggtgcaggaa ataccggaag
     1021 cggagtaatc gaaattttgc gtaacggttt tcgctgtgat gttctagctt atgatcctta
     1081 caaatcagat ttatacatcg aaagctttgg tgcaaaaaaa gtagaacttc aagaattact
     1141 ggaacaatca gatatcattt gtttatgtgc gaatttgaca gaagagaact atcacatgat
     1201 ttctgataaa gaagtcgatc agatgaaaga aaatgtttat ctatctaata gtgcaagagg
     1261 cgctctagtt gatgaagaag cgatgattaa tggtctgaaa agcggaaaga tcgcaggata
     1321 tgccacagat gttttagaag aagaaccggg acgtcaagat catccgtatt tagcgtttga
     1381 acaagtagtg attacgccac atatatcggc ctacacaagg gaatgtttag aagcaatggg
     1441 tgaaaaatgt gtcagagatg tcgaacagat cagtcagcac cttcttcctg aacgttctgt
     1501 ccagacaaag agcaactata taaaaggatg atgaagaaaa tggtgaacgg tttaaaagtg
     1561 aaaacaggcc ctcaatttta tttatatgaa gaaggtggta tttcaaaagt ttctgatctc
     1621 ttgaaatcat acggcgcgaa aagagtactg gtcacccacg gaacggtatc atgggaaaag
     1681 gcgctaccta aattagtatt tttgaatgac gaaacgattc aattttttta tcatcgttat
     1741 tctggagaat gtagttatgc tgaagccaga cgaatagcta caatcatcaa aaaaatgaaa
     1801 tcgatttcgt agttggcgtt ggcggaggaa aactgaccga tctagtgggt tatagtgcca
     1861 atcttgcaag aatcgatttc ggagtcatcc ctactttagc tagtaattgt gctccatgga
     1921 cacctcttgc tgtcatgtat aaggagaatg gacaagcaga agggaaaaca gaacactttt
     1981 acagacaagc tgcttttttt ataacagatc cccaattagt gattgattcc cctgttccat
     2041 atttcattgc cgggttagca gatacactag caaaatggta tgaatctgag ctcatcttag
     2101 aacaagattt tttgcaagat gaatcatttt tgaagctagc acaagatact gcgaagatct
     2161 gcaaagaaga gattttgaat cattctgcta aagcaatcca agatatggca caaagaaaac
     2221 tgacatcgga gttcaaacag ctttcagaga ttatctttgc tgttgctggt ttagttggag
     2281 gattaggaga taaatacgca agaaatgtgg cagcacatgc tatgcatgac gctatcagta
     2341 aatatattcc ggaaagccat cgttttctac atggagaaaa agtagcgtat gggattttct
     2401 atcaactggc actagaaggt cgctgggaaa aaatagacga gttatatcct ttgtatcggg
     2461 aattagaact gccggtttct ctagaacaaa tgggattatt tccgaaagat aaacagataa
     2521 tagataaaat ggctgaattt attgattcga aagagaaagt gcatttgatt cctatagagg
     2581 taacacagaa atcactgata cagtcaatga tggaactgga aggttatatc agaaagatct
     2641 gaaggaggag gacacatgga aacattaagt gtagtacaaa gtttgttgat tgctttatgg
     2701 gtagccgcgg ttatgtcccg ctggttaggt ggaggagcca cgttgacctt acgtttttct
     2761 cctttgatga ctggattagt cgtcgggttg atcatggggg aagttgcaca agcgatgatt
     2821 gttacagccg ctttgcaaat gatttatatg ggggtttttt ctccaggagg atcaatgcca
     2881 gctgaaccat ctattgcagc agctatcgca gtaccagtcg cattattagg ggatctgtcg
     2941 ccagaagcag cgattgccgt tgcggtgcca gtcggattgc tcggaagtta cttgtatcag
     3001 tttcgttttt tcatcaatac ttttttaggt aaatatacag acaaagctgt agaagatttg
     3061 aatacaaaag ctatggcccg atcaattatt ctttatccaa cggttgcctc atttttatta
     3121 ttcgttccgc ttgttttcat tgctttatat ttaggggcgc cattgatcgc tgatgtaatc
     3181 aaagctttag agggaacagt cgttatccac attcttgaag tagtaggtgg agggttagcg
     3241 gcaatcggta tcgcgacaac tgtatacgtt atcggtcgaa aagattatct ggtctttttc
     3301 ttccttgctt attttatgag tgttgttttg aaatctttag atattacgat ggttacttat
     3361 gccatttttg ggattattat cgctttgatc tttgtccagt cgcaaaaagg gaaacaagta
     3421 cagcctgcca caagcaataa caattcagca tcttttgatg acgaagatga ctatgatgac
     3481 ggattttaat ttaggaggaa tgggaaatgg ctgaaataat ggataatcaa acaggtgctc
     3541 ctgaagaaat cacgaaaaaa gatgtaacaa aagcctatct gagatggcat tttgcaaacg
     3601 aaattcctca ctcttttgaa cgttacttgg cgccatcact actatacgga atgatgccaa
     3661 tcttgaaaaa actttataaa gatgaagact ctttaaaagc agcatataaa cggcagttac
     3721 tgttcttcaa cactcaatta agctggggtg gcggtgtgat tactggccta atgtcttcta
     3781 tggaacagca aagagcagaa gaagagtatg aagaaaaaga gatcttgatg caggatgact
     3841 tgatgtataa cacgaaagcg ggattgatgg gggcactagc aggaatcgga gatgcaatcg
     3901 attcaggaac cgtccaatat atttttatcg caatcgcggt accttgggca caacagggaa
     3961 gtcctttagg cgcacttttt ccttttatct gctttgggtt gtatcaggtt ttactagggg
     4021 tatttttcgc aagacaagct ttcactatgg gccgtaatgc tacaggccta aagcaaaaca
     4081 ctggtgttca gacagcgatt gaaatgctat cagttttagg gttgtttatg atgggggtgc
     4141 ttgcgggaaa ctatgtaaaa gtagaatctt cattgaaatt tgcgatatcc ggacgtgagt
     4201 ttgttatcca ggaaattctt gatcaaatcg tgccagggat attaccttta gctgtcgtaa
     4261 tgggtgtgta cttgttctat acgaaaaaag gattgaaagt cacacaagca ttattatggc
     4321 tgactggaat attaattgtg ctagcaggaa tcggtatttt ataaaaagaa gggaacgtga
     4381 ataagatggg tgtagtcaat ttagcgcgtg tggatgagcg tttgatccat gggcaagtaa
     4441 tggtaacgtt atctcaaaaa agtggagtga attccatttt tgttgtagac gaagttgtag
     4501 caaaagataa atttatgaga gatctttaca aaaatgcagg aagccgtaca ggacaaaaaa
     4561 caattgtcat tacaccagag aaagctaaat tctattggga tgaatatcaa tttaaagaat
     4621 acaattgtat tttgatcgca aaaacagtcc aaacgattta cgacttagta aaacacggaa
     4681 tccccatgga agaattgaat attggaggga ttgctcaaaa aaatcctgat acagatcttt
     4741 tagtgacgaa atctgtttat ttaaataaat cagatgctga aaaattgaaa gaaatgaatg
     4801 aacaatatgg cgtaaagaat atttattttc aagccactcc atctgcacct aaaacaggat
     4861 taaaagatgt attgaacaag tttgatttgt cataaacaaa tgcttagaaa aaagataaag
     4921 agggcgaaaa aatgaagaag aacgagctgt ttcgtgattg ggaatttcgt tatcgctata
     4981 tttaccgtaa gcgccgtaca aaaaagagta aacagcgttt tctttcagca ttagtatcag
     5041 atatttacag tatgagaaca gatgtgacag tcattgctta cgatacgcta gcctaccgat
     5101 caaaaaatat ttatgttgga gatatcgaaa aagcagagaa agttatctgt acttattacg
     5161 atacacccgt tcatgcatta ggaagttatt tcatgtttga ttggaaagac caaaggaaaa
     5221 aaacaatcta ctctatatta ttgtccttca ttttgttgtt cagtttaggt tggtggggaa
     5281 tgatgatata taacaaaaat cctcatcacg tattcgatct gctatccgta taaacaggta
     5341 tcactgtact agcatttggg agctactttt tcttattggg aaaagcagct cgcggatggc
     5401 ctagcagaca aacttttatt cgaaatactt catccatatt aacgatgcta gaaatgatcc
     5461 gtacgattga tgatccgagt gtagcgtatg cgtttgttga tgaaggctgt tatggagaaa
     5521 agggcttaga cagcgtgaga tcgggtatga aaaaagaggc tattcttttt tatctagact
     5581 ctgttggggc agatacaccg ctacagtttt cgggaaatta tttttcaaat aaagaacagt
     5641 ggctgaaaca agttgataag ttgaaagaaa aaaacgtcaa ttatattttt agtgcaagaa
     5701 aaaagcaagc gcagttcttc tatttgacca aaacagattt acgagggaaa acatttaatt
     5761 ggcaaaatgc caatcagatt attgctcttt ttcgataagt aaaaagtgca aaacaaggag
     5821 tgagaagact aatgttggaa atcatgatcg ttacacatgg taagttgagc gaaggactga
     5881 aagatgcggc ggaagtaatt gtcgggacat cgagccatat tgaaacaatg tgcttagagt
     5941 ctggggagga catccaaaca ttcggagaga aggtcaaaga aaaattaact atgatcaaca
     6001 cagcagaacc catgctagta tttgttgatc taattagcgc aagtccgtac aatcaagcgc
     6061 tcttggcagt caatgaactg actgaagaaa agaaagcagc cacttatgtc atagggggcg
     6121 tgaatctgcc aatgttgtta gaagcaatca atcatcagat gcttcacaca ccgatagaag
     6181 aagcggtgga acaaattgtc aaacaaggaa aagataattt aactgtttgg catgcttcag
     6241 cggaagaaaa aaaattagat gaggacgatt tttagaagga aggctgaaaa gatgaagtgg
     6301 ggatttagat ggtatggtga aaaaggggat tcgatcccat taaaatatgt tcgccaaata
     6361 ccgggaataa atgggataat tggaactttg ctgactaaaa tgcctggaga tgtttgggag
     6421 atttcagaaa ttgaggagtt gaaagcatct gttgagagcc aaggactgga actgcttgga
     6481 attgaaagtg tagcgatcca cgatgcaatc aaggcaggga cagatgaacg agattactat
     6541 attgataact atatacgaac aatcaaaaac ttgtctcgat gtggggttcg attgatttgt
     6601 tatagcttca aaccaatttt tggatgggcg aaaactgatt tgtttaatga aaacaacgat
     6661 ggaagctatt ctcttttata cgatcagaaa atcgtcgatc agatggagcc aggagaaatg
     6721 tatcaattga tacacagcca gtcaaaggga ttcaaattgc cgggttggga agaagaacgg
     6781 ttgaaaaaat tcgctcagct ggaaaaactg tatgaaggaa tgaccgaaga agaattattt
     6841 gaaaatctga cttatttttt agaaaaagtg attccagtgt gcgaaaaagc agatgtaaaa
     6901 atggctatcc atccagatga tccgccatgg gaactttttg gttttcctag agtgacgaaa
     6961 aatcttggcg acttgaagaa aattctaaat gcagtagatt ctccatataa tggactaacg
     7021 ctgtgcacag gatctttagg ggcaaatccg gaaaacgatt tagttgaaat catccatgag
     7081 gtaggttcgc gcatccattt tgtccacttc agaaacgtgg agtttttagg agaaagaaag
     7141 ttcaaagaaa cggcacatcc ctctacagaa ggctcccttg atatgtatca tattatgaaa
     7201 gcgttggtgc aagaagggtt cgacggtcct atacgccctg atcatggtag aaccgtgtgg
     7261 ggtgaagtgg ctatgcctgg atatggcctg tatgatcgtg caatgggact cacttacatg
     7321 caaggtctat atgaggcgat aagtaaggaa aaaccactgg gagagcatta tgaataaaat
     7381 caagctcttc atctttgata tggatggcct tttattagaa actggacgtt tagcttatcg
     7441 tgcttatgta aaatcagcac aaaagtatga ttatgaaatg cgtaaggaag tctattattt
     7501 actaactggc caaacagaaa tggcgataag agaacaaatg ggcattttgt acggagaaga
     7561 tgttccatat atcaaatggc gagaagcaat caatcaatat aaagaaaaaa tcgtgaaaga
     7621 agataagaga gtatatacga aaaaaggagc agaagaaatt ttatcttttg caaaagaaag
     7681 aggaatccat acaatcgtgg cttcgtctaa tacacgggta aaagtagaaa tgtatttaaa
     7741 aatggaaaat ctatatgcct tgtttgatca aatcatttca ggggatgatg taaaaaaagg
     7801 aaagccggaa ccagagatct ttttgaaagc ttgttcaaaa atgaatattc ctccaagtga
     7861 agcacttgta tttgaagact cgattgcagg gatcgaagca gcaaggcgag cgggcatttt
     7921 gtcttttctc atcaaagatc atttgatagg gttgcccgat cataatggga agcataagtt
     7981 gaaagtagat gtaagtttat atgaatccaa taaagcagac tatgaatttt atgacctgca
     8041 tcaagcaaaa cgatttttag aaagtaaagg gttgtatctc taataaagag gactattttc
     8101 tagtcctctt tatttattgt cctgcacgac tgagggaaca gatgaaaaag aagtaatttt
     8161 ttattttgaa gtatctacct tatttgtagt gaacaattta gaacaaacac aaaacgcatc
     8221 tactgttatc taccagtaga tgcgtttaaa gagaagaacg agaatcgtcc atatgagggt
     8281 ttccactcct tatgcagatt tttttaattc ttgcactgct tgttcgatcg taacacctgt
     8341 cgctacatga ttggtatttt ctttgtcaaa aacgacaaat agattcaaat ctgtatccaa
     8401 ggttactcga tattgtatat ctttgttata gaatgtcata gccattctcc tttctttaaa
     8461 tacttgctga aaggcaacga acgcgttgat aacgaaattc catttataag tataccacca
     8521 attgaaaggt tttcatagtt aagaaatttt aaagtcaaaa gttgcagata aaagcaaata
     8581 aacaagaaaa caaaaagtga atcaatcgcg gttattcaac tttttcttcg attcgaagac
     8641 aatagaagac aatgaatttt atatttcatt gcattatcct tgtgttatta gaaaaagcat
     8701 gatatattaa atgagtaatc tattttgaaa cgtaatttga gcgatatatc tacatagata
     8761 aaactccttt tgcctatcaa atatttattg caacgaaaaa acgtacattg atacgtatca
     8821 ggaggaattt atttatgaat aacggtacag taaaatggtt caacgcagac aaaggttttg
     8881 gctttatcac tggtgaagat ggaaatgatg tatttgcaca tttttcagct atccaaggtg
     8941 acggcttcaa aactttagaa gaaggccaag cagtgtctta cgatgttgaa gatggtcaac
     9001 gtggccctca agcaacaaac attgttaaag cttaataaaa attgactttt aaccgtcttt
     9061 tcgaaagaaa a
//
LOCUS       DACECR010000103         8555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.104, whole genome shotgun sequence.
ACCESSION   DACECR010000103 DACECR010000000
VERSION     DACECR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.104"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(44..1510)
                     /locus_tag="IWO17_002375"
     CDS             complement(44..1510)
                     /locus_tag="IWO17_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ0291232.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1897..2493
                     /locus_tag="IWO17_002376"
     CDS             1897..2493
                     /locus_tag="IWO17_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0291233.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2551..3033)
                     /locus_tag="IWO17_002377"
     CDS             complement(2551..3033)
                     /locus_tag="IWO17_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291234.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3139..3336)
                     /locus_tag="IWO17_002378"
     CDS             complement(3139..3336)
                     /locus_tag="IWO17_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291235.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3631..4389
                     /locus_tag="IWO17_002379"
     CDS             3631..4389
                     /locus_tag="IWO17_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ0291236.1"
                     /translation="MKKKTFKKLLFLGGTTALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4449..4877
                     /locus_tag="IWO17_002380"
     CDS             4449..4877
                     /locus_tag="IWO17_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291237.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4923..4995
                     /locus_tag="IWO17_002381"
     tRNA            4923..4995
                     /locus_tag="IWO17_002381"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4956..4958,aa:Gln,seq:ctg)
     gene            complement(5145..5516)
                     /locus_tag="IWO17_002382"
     CDS             complement(5145..5516)
                     /locus_tag="IWO17_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0291238.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5731..5916
                     /locus_tag="IWO17_002383"
     CDS             5731..5916
                     /locus_tag="IWO17_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291239.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(5999..6187)
                     /locus_tag="IWO17_002384"
     CDS             complement(5999..6187)
                     /locus_tag="IWO17_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291240.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6211..6474)
                     /locus_tag="IWO17_002385"
     CDS             complement(6211..6474)
                     /locus_tag="IWO17_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291241.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6632..6889
                     /locus_tag="IWO17_002386"
     CDS             6632..6889
                     /locus_tag="IWO17_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ0291242.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6960..7151)
                     /locus_tag="IWO17_002387"
     CDS             complement(6960..7151)
                     /locus_tag="IWO17_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291243.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7292..7612)
                     /locus_tag="IWO17_002388"
     CDS             complement(7292..7612)
                     /locus_tag="IWO17_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291244.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7635..8069)
                     /locus_tag="IWO17_002389"
     CDS             complement(7635..8069)
                     /locus_tag="IWO17_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0291245.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8404..>8555
                     /locus_tag="IWO17_002390"
     CDS             8404..>8555
                     /locus_tag="IWO17_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0291246.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNS"
BASE COUNT         2708 a         1687 c         1357 g         2803 t
ORIGIN      
        1 ggaactgctg caaataagca attcctgttt ttatgttcat tgattaatga atagagccca
       61 ttagtttttt gattggttct ttcacagtaa atagcaagat accaccgacg attgctactg
      121 caccgataat accgaagtaa gcagattctg ttcccggtgt gtagaagcgt gcaatttgtg
      181 cattgattgc ttgtgaagaa gcatcagcta agaaccaaat agcgattgtt tgtgattcaa
      241 atgctcgtgg tgctaatttt gttgtcacag acaaaccaac tggtgaaaga cacaattcag
      301 caataaccac gatgaagaaa cttcccaaca accacaatgg acttactttt ccacttgtac
      361 cataaagcat acccggcaac atcaatagca agtacgataa tcctgcaaat aaaagtccaa
      421 gagagaattt cactactgta gatggttgac gttcaccaag tttcgtccac atcgttacga
      481 atactggtgt cagaataaag atgaaaattg gattcaatga ttgataccag cttgcttgga
      541 tcgttacacc aaatagcgta gattgtgtac gttcactagc aaataaagct aagatagatg
      601 aaccttgttc ttgtaatgac cagaaaatga ttgctgcaag gaataatggg atataagccc
      661 atactcttgg tttttcttca gcagttacat cttttgaagt cagcattttg ataaagtaat
      721 acaacggtaa gataatcccc aatacactga ttgtattgac aaaaaagtca attgtcagat
      781 gaccagttag ttgtgccccg ccgaatactg cagcgataac aacaagtgcg atgatcaagt
      841 tacggatgaa tcctttacgt tcttcagttg tcaaaggatt cggcggtgtt gtacccactc
      901 cgtctaatgt cttgcgtcct tggatgatgt attgtaataa tccaaagaac atcccaatag
      961 cagctaaaga gaaccctaaa tgatagttat attcttgacc gattgtacca acgattagtg
     1021 gtgctaataa tgcccctaag ttgacaccca tgtaaaaaat cgaaaatcct gcatctcttc
     1081 gcaagtcagt ctttgaataa agatgtccga ccatgccaga aacatttggt ttcaaaaagg
     1141 cggttccgat aataatcaat gccatcgaaa caaacaatgt acttacacca aatggtaaag
     1201 ccaagacgat gtggccaagc ataatgaaaa ctccaccgaa gaatacagtg cgttttgctc
     1261 ctaacactcg gtcagatacc catccgccga cgatactaga catgtagatc aaagatccgt
     1321 agatcgacat gatagctaat gctgttgctc gtggtaatcc aagtcccccg ttggcaactg
     1381 tatcgtacat ataatagata agtaatgcac gcataccgta ataactaaat cgttcccaca
     1441 tctcagtgaa gaacaaagtt gacaatccct tcggctgccc gaagaagccc ttgtcttgct
     1501 gtttttccat aatctatacc ccaattcttt aaattttcag aaaatattat acatctttac
     1561 cgaaaaattt aactaaaaaa cttatttaag aaatataaac taattttcta taaaagcaag
     1621 taatttttaa tggcaatatc ataaaaaaaa gaagatagag atttaataat tatatatcag
     1681 ctgttttttt caaataagca aaaattccta aaacacaaca atgaaagcga taactaagaa
     1741 aatccttaca taacgatttt tcttattaaa attgtttttt gaacttcgct tattttttgt
     1801 gagcatatct gtttacttac tttttgtaag gtgctatact tcatttcgta atcaattaaa
     1861 aaaatgcaaa acgcaaatca aaggagaaat tttattatga caacttttac tgacactttg
     1921 aaaaaccgcc gttcaatcta tcatctagga cgtaatgttt cactttctaa tgaagaatta
     1981 acaactttga tcaaagaagc aattaaagaa agcccaactg ctttcaatgc tcaatcaaca
     2041 cgcgcagtta tccttttcgg agatgcacat gaaaaacttt gggaaatcac tgaagaagca
     2101 cttcgcccat tgactccagc agaagctttc ccaaatacac aaaacaaatt agctggtttc
     2161 aaaaatggtt atggtacagt attattcttc aaagatactg acgtggtaaa aggtctacaa
     2221 gaacaatttg aattatatgc agacaacttc ccagattggt cagaacaatc aaacggaatc
     2281 gcaacagcta atacttgggt agcattagtt gataaaggat tgggcgcaaa cttgcaacat
     2341 tacaacccag ttattgacga agcagttgca aaagaatgga atatcccttc aaactggaaa
     2401 ctacgttcgc aattagtatt cggttcacca gaaacaccgg ctggggaaaa agaatacatg
     2461 aacgatgccg atcgcttccg cgtatttgga taattttatt tgaaataaaa aacagaggtt
     2521 gtgacaaaca tcttgtcgca acctctgttt ttattctgga tcaatcataa tatcaatctc
     2581 tgcctcatcg tttaagcgtt gggacaaaaa tggaactaat tcttctaatg atgcagaaaa
     2641 tttcagcatc ggtcctcgcc atccgtttcg gaaaaaattc atttttacga ttttagaacc
     2701 ttttggctga tataatacaa ttttattgat ctcagaaaaa ggaacacctt ttttgtcaaa
     2761 gctattcaaa acaagtcctt cttctgtgat tcctctactg tccaacaaaa aactaataaa
     2821 aatcattgct gacaagatcc cttttgccaa ttcatctgct gtaggatgac tgatgagtgt
     2881 ataccaaatg actagtgaca ctaagaaggg tatcaccaag tagacccatc gatttcgcac
     2941 agtccgataa ataatttctt ttcgaaccag ccaaagaaat aaaaacatca caacaacgaa
     3001 taaaaacaat agaaccgtaa agaatgttac catgacaact cgcctcgctt tttcttctcc
     3061 agaaaatagg aaaagaatac tgatttacct tctcaaagaa attggacttg cagcacgccg
     3121 cacgtccaat tgttttcttt attcaaatgt atcatatttt gcaacaacca tgcttgcttt
     3181 ttcatctaat aattcttggt cctctgcagc acattgggca actttgacca cggcgcagtt
     3241 tgtcattttt gatacaactt caccaatgac cgtttttgta aaaccaattg gtttgcatgc
     3301 atattgttct cctaaattaa tcatttcttc cgtcatcaaa atcctcctaa actgctggaa
     3361 cttttctcca cattaccact gctatttctt tggagattta actcattgat ttttatttac
     3421 agttaaaaat caaccttaca ggcattatag cgtactgttt tcgttttcac aattaaaaca
     3481 gctgttgtat ttagaagaat ttttttcaaa aaattaaaac atattcctta aaaactaatt
     3541 atttcctaca tattaaaata taatacttcc atttccaata aaaatacatt tataatagga
     3601 aagataaaca gctgaaaagg gagatttcaa atgaagaaaa aaacgttcaa aaaactattg
     3661 tttctagggg gcacaactgc tcttttatta agcggtacaa gttattacct acatatccat
     3721 tctccagaac atacaatgaa cactgcacaa aaaagttcaa cacaaaaaac gaaaaagaca
     3781 aaagagaaag cagaagtttt actagatgtt ccattagaga gccagtttga ttctccttct
     3841 ttagaaaatg gctgtgaagt cacttcgctt tctatgcttt tatcttttta tggttatgaa
     3901 acaactaaaa accagttagc tgagcaattg gattacgtcc ctgttttcaa tgcagatggg
     3961 actcatggcg atcctaatga aggatttgtt ggtgatatca gtggcggaga ctgggctatg
     4021 gtcgtctatg ttcccccagt tgcctccctt gctcagcaaa tcgtccaaac ggattatcac
     4081 acttttccaa aaaccgatgc tcaactcgat gatatcaaaa aagcactatc taaaggaaat
     4141 cctgtctgga cttctgtgac tatcaatttt gaagtcccag atgaaactga ttttatgact
     4201 tggaccacaa ataatggaga agtaagagtc actcctcttg ttcatgcttg tgtagtcact
     4261 ggatatgaca aagaaaatat ttatgtcaac gatccctatg gtgtaaaaaa tcgagcagta
     4321 ccgatcaacg atttttcggc gattttcact gcaatgggtg gacagatgct aactttggag
     4381 aaaagctagt aattcaaaca aaaatccctt atactcaaga taccaaacct ttgaagggag
     4441 cgtagataat ggaaaaattc acacataaaa aaatggatcc aaatgaaatt ccgattattt
     4501 ttgtccgtga ctgcaaggga aatgttcaag gtaaagtctc tattaatgag tggaacgagc
     4561 gtcgcagacc tgctaccctc aatgaactgg aaatcaaatt gtatcgtcaa tctcttgttt
     4621 attacgcaga tcaagagtac gagaaagcca ccgatttatt gaaatttcta attgctcgaa
     4681 cagagtatac tcgctttgaa tatattgaac gtttagcaaa catctatcat attatgaatg
     4741 aacctgtcaa agaatatcag ttattggaca ccgtcttgtc agtagcagaa ctgattgccc
     4801 ttcctgctgg attagaaaag aaactagtac gccgcctttt acgagtaaag caacaactat
     4861 ccgatcaaga aaaataactt gcgttttttt aggaaagtgc tatgattatt ttgttgaata
     4921 aatgggatgt agccaaactg gtaaggcact tgactctgaa tcaagcaatt gtaggttcga
     4981 accctgccat cccaatttga tgtgcttgct gaaaaaatga accccctgta aaagaacaat
     5041 ctttttgcag ggggattatt gtattatttt tactacttgt atttagttta cataattaaa
     5101 ttatgaataa taaaatcggg cgtattctta atacaaaaac tgatttaccc aataattgct
     5161 aatgctttct ccggaacttt ttctctagga acttcctcat acgtttctat gtgtgctcct
     5221 ttattgctaa ttttcaaata atgattttct tttaacacag aaagaccatt aaattcaacc
     5281 gttcgttctt tcccattggc atcagcagct ttctgcacat accttgctgt accatattca
     5341 tttacttcct ctggtttttg tgtctttaca tagacctctc ctttttcaac taatggattt
     5401 agctgatcaa gtataccagg gatttctcca taacttcctt cagtataata tttcagtcca
     5461 aatagcaaaa taccactaat cgccgccagt ccaatcaaat atttaatcac tttcatcttt
     5521 ctacctcctt gttatatatt cagtatatgg gtcagttaaa gaaattccat ccgtcccaag
     5581 ttggagattt ttctcctact ttagaagcga gaatcgagtg tttttttagt aaaaatgatc
     5641 gttatttgat attattgacg tgtggataaa tcagtttttc tcgttttgtc catgtaaaaa
     5701 atcatgttga tagcaaagga tgtgttcaaa atgaatcaag aagaattatt tcaaaaagtc
     5761 aaggaaatga ttaagaacgg caatttcgat ggcgcaaaac gatttatcga agaacataaa
     5821 gaacaattag gaccttataa agagaaagcc caaaatcttt taaaagatgt aaatatcgac
     5881 agtgtcaaaa acaaatttaa aaatctattc aaataagtaa aactttcact tttgtaaaat
     5941 caaaggagga caatgctgct gcatcgtcct cctttggttc ttttctgaat acttcatact
     6001 aaaataaacg tgtcggatcg taatgataag tcacattgcc gttcagtgag gcttttccca
     6061 ccacatattt acgattctcc aacttcagcc acgcttctcg aaaagccatt aattcttctc
     6121 tttttacatc aatcttttcg atttttccat cgatcaaatc ttgaataagt tgttcatatg
     6181 gattcatttt tttcctccat ctgctgtcaa tcaataagtt gtaaaaatag gttcatatgc
     6241 ttttttggta tctagatctt ctctgacttt gactgtcgta taaataattt tttctactgg
     6301 gatactataa taacttcctt caccattatt gaatcggtga acgtctttct ttgctaaaat
     6361 accatttatt ttattcgtca gtacacgttg ttcgacactg ttacttacaa catagtaaat
     6421 cacccgaata ttattgatca aatgcaaatc gatttctaac actacatgat ccatgaaaat
     6481 cccactcctt ctttcacttt gattatagca agtttctcct ctaatttcaa aatcgataca
     6541 gattttaccg gataaatcca caaaactgct tgcgccattt cgcacaaagt gttattttgt
     6601 atcatggact ttatttatcg gaggcaatag tatgatcgcg atcggtcaat ttgtttttta
     6661 tattcctttt tttattatga taagcattct tttctattac ataaaatgga caaaaaagaa
     6721 gttttcagtt ttactggcct ctttacctgc tgtatatttt acttatcaaa ttttttcttt
     6781 taggcattgg gaaacaacgt ctgtattaat aacacacatc atcgaattga cgctttcagt
     6841 aatttttctt attatttgga tctatttttt atataagaat cagaattgaa tccctcgcat
     6901 gtttaaaaag aaaacgatta aagatgcagc aaatttctgc cgatctttaa tcgttttttc
     6961 tattctgtta taccaaaaaa atgtttgaat cggtgaagga tcgttccttg ttttaattct
     7021 ataaattctt gtgcgatcgg gtacttttct tccaaatttt ctttgttctt ttcgatcagg
     7081 cttttcgctt cttcatattt tttatctgca catagtgctt ttacttcctg taatactttt
     7141 cctgcttcca tttgcctcac cctcttgcca ttttattgct ttaagtttta tttaagttat
     7201 acgcattatt ctagtttcat cccaaataac cttttttcat ttttactcct cgaactaatc
     7261 agttttctaa aggctgattg gttcgtcttt tttatttttc ctccatttca ttctgctctt
     7321 ctgttaattt atctaatgct ttgattgatg ctgcggctct tgcataatca tagctgtata
     7381 attccataaa tttatcaggc agaacaaacc acgctggatg ataaccgtta gctgtgacag
     7441 ggaatccttt atcttgttgg ttatctccat cccatctcag agcaacacct ttttttcctt
     7501 tccactttac taaagcgatc gagtatccct tctttgtttg tgtgccgtct tcaataattg
     7561 ctaaaatttc tattttttct tttggcgcac ggacttcttg aggacgaacc attttctatt
     7621 cctcccttat ctcattactt cacgaccaac acactgcaag gggcatgtga tacgacataa
     7681 gaagcggtcg atccaactaa tgcttgttgt atagcacctt ttcctgtcgc tcccataacg
     7741 atcaaatcaa tattttcttg tccagggatg acattggcaa tcaaatttct tggatctcca
     7801 gtttcaacta ttgcgtggat ctcagcaatg ccgtactctt tggcatcatt gatttttttc
     7861 agcatggcta cttctgttct ttgtttttca tctgcataga tttttgaaaa ggcataggca
     7921 ctatgggtca gctcaacttt gttgatgatt gacacgataa ataagtgggc ttcattgtcc
     7981 tttgctaagt caagggcttc agcgaacgct ttgatcgatt gtcttgatcc gtcaactgct
     8041 accatgatgt tctggtatct tttactcatt ggaaccgcct cctttttctt ttattgtaac
     8101 atctcaagca aaaatacaaa aaatatacgc aatatgtaaa taaaatgatg aacaaaagca
     8161 atttctttca agttatttat acatttcttt tctcatcaga acacctgttc taatcccatt
     8221 atttctgcat tatgtgaaat tcctcatcat ttttcacatc gagcgaacat cattctgtta
     8281 caaaaaacgt ggatttgtca taaattgtca tgcttctttt acctatgaaa tattttcacg
     8341 tgttaaagta ttcctcgttg aacacaacaa ataaaacttt ttaacgaata aaggagattt
     8401 ttcatgacat cacttaaaac tttacttttt ggaacaactt tggctgccgg cgctgcattc
     8461 ttcatgggaa cgactgctca tgcggacgaa gcttataccg ttcaatcagg tgatactcta
     8521 tcaacgattt ctcaaaaata cgttggtgat aactc
//
LOCUS       DACECR010000104         8493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.105, whole genome shotgun sequence.
ACCESSION   DACECR010000104 DACECR010000000
VERSION     DACECR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.105"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IWO17_002391"
     CDS             complement(<1..173)
                     /locus_tag="IWO17_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0291247.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IWO17_002392"
     CDS             complement(593..1714)
                     /locus_tag="IWO17_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAQ0291248.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /locus_tag="IWO17_002393"
     CDS             2042..3451
                     /locus_tag="IWO17_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ0291249.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IWO17_002394"
     CDS             3448..4176
                     /locus_tag="IWO17_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0291250.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IWO17_002395"
     CDS             complement(4304..5215)
                     /locus_tag="IWO17_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0291251.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IWO17_002396"
     CDS             complement(5232..6239)
                     /locus_tag="IWO17_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0291252.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IWO17_002397"
     CDS             complement(6236..8365)
                     /locus_tag="IWO17_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0291253.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IWO17_002398"
     CDS             complement(8370..8477)
                     /locus_tag="IWO17_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291254.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2682 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DACECR010000105         8335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.106, whole genome shotgun sequence.
ACCESSION   DACECR010000105 DACECR010000000
VERSION     DACECR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.106"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            264..1457
                     /locus_tag="IWO17_002399"
     CDS             264..1457
                     /locus_tag="IWO17_002399"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ0291255.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1587..3059
                     /locus_tag="IWO17_002400"
     CDS             1587..3059
                     /locus_tag="IWO17_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0291256.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3142..3930
                     /locus_tag="IWO17_002401"
     CDS             3142..3930
                     /locus_tag="IWO17_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ0291257.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3997..4665)
                     /locus_tag="IWO17_002402"
     CDS             complement(3997..4665)
                     /locus_tag="IWO17_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0291258.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4780..6492
                     /locus_tag="IWO17_002403"
     CDS             4780..6492
                     /locus_tag="IWO17_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0291259.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6495..8267
                     /locus_tag="IWO17_002404"
     CDS             6495..8267
                     /locus_tag="IWO17_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0291260.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2551 a         1391 c         1828 g         2565 t
ORIGIN      
        1 gaagatactt tttgagtatc tttttctttt tatctgaaag aatgatttta aaaagcttag
       61 cagagttatc tttatggatg attgtgctaa gcttttttgg acagttctaa tgtaaaatat
      121 ttttatattt tttaagcaaa aaaatgaaaa aatacgagca gaaaactgtt ttatctgcta
      181 tacttggttc gttgcaagtt cccgaaagga tccgcaggac ggagatgcct tgtaaccgaa
      241 tttaactagg gggaatcatt ataatggtag aaagacactt atttacatca gaatctgttt
      301 ctgaaggaca tccagataaa attgctgacc aaatcagtga tgcaatcttg gatgcaattt
      361 taaaacaaga tccaacagca cgagtagcgt gtgaaacgtc tgtaacaact ggtcttgttt
      421 tagtatttgg agaaatatct acgacggcat atgtagatat tcaaaaagtc gtacgggaaa
      481 caataaaaga aattggttat acacgtgcaa aattcggatt tgatggagat acagcagctg
      541 tattggttgc gattgatgaa caatctcctg atattgctca aggagttgat gaagcacttg
      601 agatccggga tgaagataaa aaagacgtat tagatgagat tggtgctggt gaccaaggtt
      661 taatgtttgg gtttgctgta gatgaaacac cagaattgat gcctttacca atcgctttga
      721 gtcatcgtct ggtacgacgc ttggcagatt tgcgtaaatc aaatgaatta acgtatttac
      781 gtccagatgc aaaatctcaa gtaacggttg aatatgatga tcaaggacaa ccggaacgcg
      841 tagatacaat cgttatttca acacagcatg atgatgcagt agataatgaa acaattcgtc
      901 atgatgtcat cgaaaaagta gtgaaggaag ttattccagc tgaattatta gatgatcaaa
      961 cgaaatatta tatcaatccg actggccgat ttgtcattgg tggtcctcaa ggggatgccg
     1021 gattaacagg aagaaaaatc attgttgata cgtatggcgg ttatgctcgt catggtggcg
     1081 gtgctttttc tggtaaagat gcgacaaaag ttgaccgttc tgccagctat gctgcacgtt
     1141 atattgctaa aaacattgtt gcagcagggc ttgctcgcaa agcagaagta cagctagctt
     1201 atgcgattgg tgttgctcaa cctgtttcga tctcaatcaa tactttcggt actggaacgg
     1261 ttccggaaga agaactgatt gctgcagtga gagaaaactt cgatcttcgg ccagcaggaa
     1321 tcatcgagat gcttgatttg cgtcgtccga tttacaaaca aacagctgct tacggtcatt
     1381 ttggtcgtac agatgtagat ttgccttggg aacatacgga caaagtagat gcgttgaaag
     1441 caagtttagc aaaataagtt atattcagct ggaacgatcg ccaagtggtg gttgttccag
     1501 ctttttttat ggcagttaac ctcttgaaaa agatagtaat tatttatcta tttttagatc
     1561 ctgtcataat gaaagggagg aataagatga aaaaagaaac aaatgttact ttggtcacag
     1621 tgagtatttt cattgcaacg ttcatgacgg ctattgaagg tacaatcgta acaaccgcta
     1681 tgccaacgat tgtcggttca ttacatggaa tggaaatcat gaactgggta ttttcgattt
     1741 atttgctgac aaacgctatg cttactccga tttacggaaa acttgcggat aaagtgggac
     1801 ggaaaccaat ctttattttt gggacattat tgtttattat cggttcgtta ttttgcggtt
     1861 tatctcaaag catgattggg ttgattgctg ctcgtgcgat tcaagggatg ggtgctgggg
     1921 caatgatgcc agtagctttg acgatcatcg gcgatcttta ttctattgaa aagcgagcaa
     1981 aagttttagg tttgaatagt tcagcctggg ggattgccag tgtttttgga ccgcttgcgg
     2041 gaggattcat cgttgaaacg atcagctggc actggatctt ttttatcaat gtgccgatcg
     2101 gaattctatt aattattttg atttggctat atttaaatga accaaaaatc atacatgagt
     2161 ctaaaccaat ggatattttt ggtagtttta gtttgatggc catgcttgtc tcattgctgt
     2221 tagggttcca attattagga gacgaaggaa tgagtccgct tgtttctgct ttgctgctag
     2281 gtagtatcgt aatgttaggt atttttgttt ttgccgaaaa aagagctaaa gatcctgtca
     2341 tcatgatgca tttattcaaa aatccactat ttgttgtggt caacttggct gcagcattgg
     2401 ctagtggttt tttgatggga atcgatgttt atatccctat gtggatgcaa ggtgtattag
     2461 ggctgaatgc agcaattggt gggctggtat tagcaccatt atcactagtc tggatggtcg
     2521 gttctttcct tgcaagcaag tggatgactc aaatgaaaac tcattgggtc ttgagaattg
     2581 gactactaat cgcagctatt gggggcgctt ggttgtatct tatgcctttc agaagtaact
     2641 atttatggtt ttttgctata tcaacggtca tcggtatagg cttaggtgtg accatgacta
     2701 cctcgactgt cagtgcacaa aatagtgtat ctcgtgacca gcttggtgta gcaacttctt
     2761 ttaatacgtt agttcgaaca attggtcaaa cagtcatggt ttctattttt ggtttattga
     2821 taaatggggt aactaaacga gaacttgcag cagcaaattt aacaaatgat gcagatatca
     2881 tgaataaact ggtgaatcca caaacggcta aatatattga tgcggatatt ttgttaccat
     2941 taagaaaaat tctttatgat gggttacatt ccgtttatct agttggcttg ttactcgtga
     3001 tcattgcttt ggtcttaacg cttttcgttc atgaaaaaag tggtaaaata aaacagtagt
     3061 ttcggttaag atatgtacgt agccgaatca ttcaaattta aagttattca aaaataactt
     3121 cttactaagg aaggcgaaaa tatggcattt attcaagcaa atatttattc gaatgtgtta
     3181 gaaatggaag tcaatctaaa tgtgatacta ccgcaaacaa caaaaaaggt gattggcacc
     3241 agttcagagc aagagacaac ggatatacct gttttatatt tacttcatgg aatgggtggc
     3301 aatcatagtg tatgggaaag acgtacgtcg attgaacgtt atgtagcaaa ctacggatta
     3361 gctgtcatca tgccatctac tgatcttggc tggtacacag atacagttta tgacatgaag
     3421 tattggacat tcattgcaga agagctacct gagatttgtc atgaattatt cccgcaatta
     3481 acaagaaaaa gagaaaaaac atatgccgct ggattatcga tgggcggata tggcgcgtta
     3541 aaactaggcc ttgcaaaatc agaaaatttc gcagcagtaa tctctctttc aggcgctgtt
     3601 tctgtaggaa atcgaatgga tgatcttttg atggttcgta aagcatcttt ttgggaaggg
     3661 atctttggac cattggatca aatcgaaggt tcaaaaaatg atccgcaata cttattgaaa
     3721 gaactagtaa aaagcggaaa accagttcca agattttata tggcttgtgg ggaatctgat
     3781 tttctttatc aggcaaatca ggaaatggta tctgctatgc aggaacaagg cttggcggtc
     3841 acctttgaac aaggacctgg cgatcataac tggatcttct gggaccaatg gattcaacgt
     3901 gcattggcgt ggctaatgga agaaaaataa aaaaagagtc ttaaaaagat agctcctata
     3961 gagatcacaa acatagtgat ctctatagga gcttttttac tgctcgtttt ctcttctttt
     4021 attttgttgg cataagcctt caatataggt ttgtgcactc gttagaaaga gtgctttttc
     4081 attcaattct gcatgggact gcatactttc tttaaatccc tctaattgat tttcaactac
     4141 ataattacgc ataaatagaa ctgtttcttt caaaaattca tttccgtcca taattttttg
     4201 tcgaagctgc tgttcatgct gctgatccat taactgtcca atcaataaat tgtggagaga
     4261 ggcaatggcc tgatgacttt gggtgactga aaatccttca cgaacaagta attggatcat
     4321 ttgctccaaa tgttctaatc gactcggaaa agcgggcaca gtattgattt ctaattctgc
     4381 cccacaaggg aaacgtgtat aaaggtcata gtaattttcc atatactgca gcaactgttc
     4441 attccaaggc aaagttggat ctggaagcgc taattcttct tccattgttt cagccatttt
     4501 ttgcaagagc tgttgtttgt ttttgaaata ccaatataaa gcaggtgctt gaacatgaat
     4561 tctgtttgcg atgtttcgca tggaaagctg cgaaagagct tgtgtttcag ccaggatatc
     4621 aaaggcggcc tgaatgatga ttgcttgaga aagctttatt tccattttat caacctcgtg
     4681 tattgcatta tctttttaaa catggtaaat tgttagtcgg tctaatttta cagtgttaaa
     4741 ttagtcctgt caatttaaac aaaggggtgt aatcaacgaa tgaaattaat cttacgacat
     4801 gcgaaaaaat ataaagtagc tgtttttatt tccttgctgt ccgtagcagt catggttgca
     4861 gcagcattat ggcaacctaa gttactacag caagtattgg aagctattat tacagaagat
     4921 aatgatgaaa tgaaaacaat tggtatttac ctgatcagtc tggctttgct aggattagcc
     4981 gcaggtgtaa cgaacacgat tttctctgca aaagtagctc aaggcgtaag tgcagatatc
     5041 cgagaagaag catttcgaaa gatccagaca ttttcatttg gaaatatcga acagttctct
     5101 gctggaaatc ttgttgttcg tctaacaaat gatatcacgc aaatccaaaa tttagtgatg
     5161 atttctttgc aatcactttt tcgaattcca ttcttattta ttggtgcctt cattttagct
     5221 atgatcacga tgccgcaatt atggtggatc atcgtagcgc ttatcgttac ggtatttctg
     5281 attacggcac tttcatttac aaggatgggc aagcacttca tgatcattca aaaactgatt
     5341 gatcgtgtga atagcattgc aaaagaaaac ttactaggga ttcgtgtagt gaaatctttt
     5401 gtacaagaag aaaaccaatt gaagtcattc agcaaagtaa gtgaggactt aacaaaacac
     5461 aacatcatag tcggtacatt attttctgtg atgatcccgt ccttcatgct ggctgccaat
     5521 ttggctgtgg ttggggcgat cttttttgtg agcgatcttg ttaaagatga tcctacgtta
     5581 attggtggaa ttgcttcatt catgaactac ttgatgcaga ttatgatggc aatcattatt
     5641 ggcggaatga tgatgatgat gacttcaaga gcagccgtat cgctgaagcg gattgcggaa
     5701 attttagatg ctgaaccaga tcttacttat ttagacgttc cagaacaaga gttgacgggt
     5761 tctgtcactt ttgaccatgt ttcttttcgt tatcctggtg aggatacaga tacgcttaaa
     5821 gatatcagct tttctatcaa acctggagaa atggttggta tcgtcggggc gactggtgcc
     5881 gggaagtcaa cactagcaca attgatccct cgtctatttg atccgacaga aggcaaagtt
     5941 gaagtaggtg gtgtagatct tcgtcaagta aatgaaaaga gtttaagaca aacggtttcc
     6001 tttgttttgc aaaaagcaat tttattctca ggaacgattg ctcaaaactt gcgccaaggg
     6061 aaaaagaatg caacagaaaa agagatggaa catgcttctt ctattgctca agcaaaagaa
     6121 ttcatcgaaa agttatctga tcgttacgaa gcaccaatag aagaacgtag cagtaacttt
     6181 tctggtggtc aaaagcaacg tttatccatt actcgtggcg ttatcggtaa tccaaagatc
     6241 ttgatcctag atgacagtac gagtgcactg gatgctcgct ctgaaaagct ggtccgagaa
     6301 gcactcgatc gagatttgaa gggaacaacg acgattgtca tcgcacaaaa aattgcttct
     6361 gttgtcaaag cagaccgcat cttagtattg gatgaaggca gattagtcgg cgaaggaacc
     6421 catgaggaat tggtagctgg aaaccgtgtc tatcaggaaa tctttgaaac tcaaaaagga
     6481 acggaggagt aagtatgact gatctaataa aagcaagtaa atttttctat cactatttga
     6541 agcggtacaa actctccttt ttctttatct ttatcacagt gatcattgct acctatctac
     6601 aagtaaaggc acctcaatac atcggggaag cttttcaaga attagctaat tacgtaggag
     6661 gcttgatgca aggcgtagac gataaaagca aatttgtttc tgtaatatgg aaactcttgc
     6721 tgttttatgt actagctagt gcagccaact ttatctatag tattttgttc acacaagtag
     6781 tagggaagtc aacgaaccga atgcgtatcg gtctattcaa caaattggaa aaattgacga
     6841 tccgtttctt tgattctcat caagacggtg aaattttaag tcgttttacc agtgacttag
     6901 ataatattca aaatagtttg aaccaagctt tgcttcaagt catcacgaat gctgttttac
     6961 ttgtcgggat cttgatcatg atgttccgcc aaaatgtaga gttagcttgg gctacaattg
     7021 cttccactcc agtagccgtt ttgatagctg tcttgattat ccgcaaagca cggaaatatg
     7081 tggatcttca gcaagatgaa gtggggaaac taaatggtta tatggatgaa aaaatcagcg
     7141 gacaacgtgt catcatcaca aatggcttac aagaagaaac gattgaagga ttcttagagc
     7201 ataatcaaag tgttcgcgaa gcaaccttca aaggacaagc ttattctggt ctactatttc
     7261 cgatgatgca aggaatgtca ctggtcaata cagcgatagt tatctttttc ggcggctggt
     7321 tagcattaaa tggtgatttg gaacgttcgg tggcattagg cttagttgtg acattcgtcc
     7381 agtattcaca acaatattac caaccactga tgcagatttc atctggatac agcatgatcc
     7441 aattagcgat tacaggggca agacgattga atgaaatatt tgatgaaaaa gatgaagtca
     7501 aaccagaaaa cggtaaagca tttgatggtg ttgaaaaagg tcttgcgttg cagcacgtag
     7561 attttggtta ttctccagaa aaactagtct tgaaagatgt ctcgatcaat gtggacaaag
     7621 gagaaatggt tgctcttgtt ggtccaacag gatcggggaa aacgacgatc atgaatctgc
     7681 taaaccgttt ttacgatgtc aatgatggtt cagtagtcat cgatggaata gatatccgag
     7741 attatgatct tgatgcatta cgtagacatg taggaatcgt tttgcaagaa tctgtattgt
     7801 tctccggaac gatcaaagac aatatcacat tcggcaaacc ggacgcttca gatgaagaag
     7861 tcatcaatgc agcaaaacaa gcgaatattc atgacttcat catgacgtta gaaaaaggtt
     7921 atgatacaga gatatccgaa gaaaataact tattcagtac aggtcaaaag cagttgatca
     7981 gtattgctcg aacgatcatc acaaatccat ctttattgat tttggatgaa gcaacaagta
     8041 atgtagacac ggtaacagaa gcaaggatcc aaaaagcaat ggatgaagca atcaaaggaa
     8101 gaaccagctt tgtgattgct caccggctga aaacaatctt gaatgcagat agaatcgttg
     8161 tcttgcgtga tggggaagtc attgaagaag gcagtcatca tgagttatta gcagaaaatg
     8221 gattttatgc ggaactttat cataatcaat ttgtatttga aagttaaatt aaaagaacct
     8281 cgttataacg gggttctttt tatatatttt atgttgtttg tttttataag gggca
//
LOCUS       DACECR010000106         8311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.107, whole genome shotgun sequence.
ACCESSION   DACECR010000106 DACECR010000000
VERSION     DACECR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.107"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(71..1024)
                     /gene="menA"
                     /locus_tag="IWO17_002405"
     CDS             complement(71..1024)
                     /gene="menA"
                     /locus_tag="IWO17_002405"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ0291261.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1037..2140)
                     /locus_tag="IWO17_002406"
     CDS             complement(1037..2140)
                     /locus_tag="IWO17_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0291262.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2230..3165)
                     /locus_tag="IWO17_002407"
     CDS             complement(2230..3165)
                     /locus_tag="IWO17_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ0291263.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3467..5401
                     /locus_tag="IWO17_002408"
     CDS             3467..5401
                     /locus_tag="IWO17_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0291264.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5858..6274
                     /locus_tag="IWO17_002409"
     CDS             5858..6274
                     /locus_tag="IWO17_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ0291265.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6288..6854
                     /locus_tag="IWO17_002410"
     CDS             6288..6854
                     /locus_tag="IWO17_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ0291266.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6877..7863
                     /locus_tag="IWO17_002411"
     CDS             6877..7863
                     /locus_tag="IWO17_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0291267.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8018..>8311)
                     /locus_tag="IWO17_002412"
     CDS             complement(8018..>8311)
                     /locus_tag="IWO17_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0291268.1"
                     /translation="SSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNF
                     NLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2457 a         1602 c         1678 g         2574 t
ORIGIN      
        1 aggagctatt tttatttggc tgttcttatt acttgatgca gggcagtttt ttcacaacct
       61 cttcatgctg ttaaatggct tgcataatga ttgttagtaa tagccccaat acataactgg
      121 cattgaataa gaccatattt ttgatggaat agctgaaact gataggctgg ggtaacgttt
      181 gcttgaattg atccaaatgc ttcttcacaa ttggcaaggt caagaaggtg accaagatcg
      241 gccattcata gataccgctc aacaacccca ttaaaatcac gaaatagcaa gcatacatca
      301 atagctggaa taagactgtt cccgcaggac gtccgatata aaaaactagt gtatagcgat
      361 gattttcaat atcacggtca agatcccgca aattattagc caacatgata ttggcaatcg
      421 taaacaccaa gggtaaggaa gctaatacga ttgctgctat agcccaaaga tttcctacca
      481 aagcaaattg tccagatgaa aagttgactg tcagatcata gacatctgaa ccgaatgtat
      541 tgacataaac gaccatggca aaaattccaa ggcccatagt caccccactg aaaatttccc
      601 ctaaaggcat tcgagataaa ggaatcggtc cgaatgtata gaatactcca ataaaacaac
      661 agataaatcc catcaaaagt aaaagccaac ccgtattgat cgttagaata acgccgataa
      721 caaaaacaaa tgcaatcata gctaaaatca tgtttcgaat caatacagga gatagatttc
      781 tgcggccaat gacattttct tcatacttat atacttcaga atgagccttt tcataatcca
      841 tgtaattatt gatagcggta gtcgctaaat caaaaactag cattccaata aaaaataaaa
      901 aagtgtttag ccaatgtatc tcatgaaaaa aagcgataga gaataatacc cctaccgcaa
      961 aaggaaatac actagccacc tttgttcgta tttcaactac ttctaaaaag atttttagtg
     1021 tcatggttcg tactccttat tttagttcag aacggtcacc catcgtgtaa tgggagtcct
     1081 ccgataattt gaaggtgtct ttgattggat cggtcacata tactttatcg tcttttgtca
     1141 cgaagatcgc ttccgtcccg ttattcaatt cgctttcgac gtattccagt ccttttttca
     1201 cacccattga aaagactgct gtagacaacc cgtcaccatc aattgattta tctgttatga
     1261 tcgtgacact tgcgatatca ttgtcgaacg gataacccgt ttttggatca aacaaatgat
     1321 ggtatttttt gccatctact tctaaataac gctcgtaaat tccagaggta actaatgttt
     1381 tgttccgttc tttaatagaa cctaaaacag atccgcgagc catgttcgga tcttggatgc
     1441 cgactgtcca gtcttggttt tcgcctcttg ggctgtggcc taagacatag acattaccgc
     1501 ccaagtcaac gatggcagtg gtcactcctt gttttttcaa gactttcact acttcgtccg
     1561 taatgtaacc tttggcgatt gcacctaaat cgatgatcat tcctttttct tttagataaa
     1621 cagttttttc tttgtcattt agttcgatct tatgataatc cactaatttc aatgcctgat
     1681 cgatttcttc ttgggaaggt tttctggcgt catcaaaacc gattcgccat aattgtgtga
     1741 ttgcaccaat cgccatatcg aatccgcctt gagaatcttg gctatattcg taggcccgct
     1801 tgactaacgt gtagacgtca tcagaaactt ttactggttt gatcccagct tgctcattca
     1861 cttcatcgat ctcagaacct ttttgattga tcgtgatttt atcaccaagt tcttttactc
     1921 gatcgaaggc aggtttcaat gctgattctt tgccttcatc atacacacgg atacgaacgt
     1981 aagtccctaa caagaactgt tcatcagaat atggatcttt caatatcttg gtagctgctg
     2041 aactttcagt ggttgtttta tttgctgagt tgctgcagcc agttagcacc ataaaagaga
     2101 tgcccagtgt gattaaggaa aatagtagtt ttttccgcat ctttttcctc tcttcatttt
     2161 gaaaggtgag acgactgcct aatctggcta tcgtcccacc ctccaacttt tattagtatg
     2221 attatgtgat tatttttctg ttacgatatt atctacttcg attgtttgag tgtcgccttt
     2281 ttcagctgca ttcactaatt gttgtgcgta catgatgaat gaatgtgagc tgtgtgtagc
     2341 acctgtaaca acttctacat tagcaggtga gccatcttct tcgcccattg ctttgacgaa
     2401 ttcgtcgttc agagtaggga tgtaagtttt tggttctgta ccgctttttg ctttcatatt
     2461 ttcattatat tctgtatcat cttgttttga tttaccgtct ttgtcgatat aatcaaagtt
     2521 tgattcagtt actttgccgc cagcaactgt catttcgaat tgcacacggt agccgttaga
     2581 gtagttcttt tcttcaagct tgtaagtgcc gtctttcaaa tcagcaccat tgtcgatttc
     2641 gattgtatct gtgttaccag cttgagcagc ttggatcaat tgttgtgcgt agttttggaa
     2701 gctttcagat gagtgagtag ctccagttac gacttccaca ccgcttgcgc tttgtgcttt
     2761 taagaattgg tcattcaact caggaatgta tttttctggg tttgtgccag attttgcttc
     2821 catgtttttg ttgtattctg catcttttgt ttttgattcg ccgttttcat tgacgttatc
     2881 atattttgat tcagtgattt tgccgtcttt tacaaccatt tcaaagactg cacggtaacc
     2941 atttgaataa tttttttctt ctaatttata tgtgccgtct tgcaaatctc cgccagctac
     3001 aacttttgca gatgattctg ttgtgctttt tgcagtcgaa gattgtgctg tagatgattc
     3061 agaagaagag tttgagcttc cgttgtcatc tgcgccgcaa gcgcctaaca ctagagctga
     3121 aaatgctaat acggcaaatc cagttacaaa acgttttttc ttcattttaa taccccacca
     3181 ttccatttct agtccacaat ttttctttgt gattcattat acattctaat ctttttagcc
     3241 tataagtcaa ctatttttga taaataaccc atgtaatatt tcaaacgttt tctttcagca
     3301 aatttcgtga aaaaacgctt tattttaaaa tttgaaaaaa atccaaaagg agttagacaa
     3361 aagaacagaa catctatata atatatggag attgtgtaat aagtatcaaa ctaaaagaat
     3421 tatcatatat tcattatata gatagtctat aaggagcgga tgatcaatgg caaagcaaaa
     3481 tattgtcgtt gtaggagcag gatatgccgg tgtttcagct acaaaatact tggcaaagaa
     3541 actgaaaaaa gaagatgtag caattactct gatcgatcgt cattcgtacc atactatgat
     3601 gaccgagttg catgaagtag ctggcggacg tgtagaacca gaagcaatcc aatacgatct
     3661 gcagcatttg tttgctcgtc aaaagaatgt acaacttgtc acagacactg taatcggtat
     3721 cgataaagaa cgaaaaatcg tcaagaccaa attaggttcc tatccttttg atcagctgat
     3781 ccttggaatg ggcggagagc cgaatgactt tggtacacct ggtgtcaaag cacacggatt
     3841 caccctttgg tcatttgaag attctgtaag gattcgggaa catattgaga agacagtagc
     3901 aaaagctgca ttggaaccag atgatgaact gcgtaaagcc atgctgactt tcgttgtctg
     3961 tggttctgga tttaccggta ttgaaatgat tggggaactg atcgactgga aaaatcgcct
     4021 agccaaagat tataaattag atccaaacga aatcacactg atggtcgtag aggcaatgcc
     4081 gacgatcttg aatatgttgg atcgaaatga tgctgcaaaa gctgaacgct atttgcgtaa
     4141 gcacaacgtg gaattattat tgaatgcacc aatcgtagaa gtggctgctg atcatatcaa
     4201 attgaaagac ggacgcaaca ttccgactca cacactgatt tggacagctg gtgtcaaagg
     4261 aacttctgac gctgctgact ttggattaga atcagcacgc ggacagcggt tgatcgccaa
     4321 tgaatatatg caggcaaaag gctacgaaga taaaaacatc tatatcattg gtgatttagt
     4381 cttttatgaa gaattcccag gaacaccgac accgcagatc gttcaagctg ctgaacaaac
     4441 aggacacaca gccgcagcga atatcgtggc ttcgatcaaa ggcggagaaa agcataaatt
     4501 taaaggaaac tatcaaggat ttatggtttc tattggtgcc aaatgggggg tagctaacct
     4561 atttgataag atccatttaa gcggattttt agctatcgta atgaaacata tcgtgaattt
     4621 gaaatacttc tttgatattc gttctggtta ttatatgttc caatatttaa tgcatgagtt
     4681 tttccatatc aaagatgatc gcaacgtgac tcgaggacat tcttctcgtt acggcaatgt
     4741 cttatggagt gtgccacttc gtattttcta tggtttagtt tggttcgttg aagcttccaa
     4801 aaaaatcatt gggaatggtg aagtgttcaa accaagtact tggtttggtg aaggatcatg
     4861 gttcactgga aacagtgtgt tcccttggac atgggaatac aatgtaacag atgttgccag
     4921 tggtgcttct gctgctggtg atgcaaccac agctgccagc ggagcagggg caagtgccgg
     4981 agaagcagcg actcaagctg cacattttgg tttgagttat gcatacggtg aagaaccaat
     5041 ggctgtgatt gagaagtcac cagactggtt cacaaagatc atgcaagtga tcatgccaaa
     5101 caatgatgta gcaatgttct tccaaaaatt catggtattc tttgaaattg ccttggcatt
     5161 agcattgatt gctggattat tcacatggct atgcagtgcg acaacgattg tattggtgat
     5221 cacgttctgc ttatcaggca tgttttactg ggtaaacatt tggttcattt ttgttgcttt
     5281 cgcattgatg aacggttccg gacgtgcgat tgggttggat cgctgggtaa ttccatggct
     5341 acaacgtaca ctaggtaatt ggtggtacgg tagaccaagt tcccagtacg gcaagaaata
     5401 aatagataaa gaacgttatt tgaatgcaag atttcaatag aaaggggctg agacaaaccg
     5461 tcacagcctc ctttttatgt aaaattaaag gaataagatg cccgagataa gcctccatga
     5521 gcaagacaca ggtgtcacaa cctctgttac aacctcttgt tataaacggt gttccagaag
     5581 tagcttcttc gaaaaataag ccgaaatttc acaaaaactt gaaaagtaat tttcgtaaat
     5641 tccttcttat ttcttgaagt tgcccacttc tgtcacaacc tcttgttata aacggtgttc
     5701 cacctgctgc ttctcggtaa taagcccaaa gaattagaaa acatggaaag ctgttttcta
     5761 ttcttcgttc ttattactca aagcaagaca caggtgtcac aacctcttgt ttttatgcta
     5821 aaattgggtg agttaatctg gaaaaaggag aacaaaaatg agccttaaag aatttgctga
     5881 aaaaagtctg ctcaaacctt gggatgtttt tattattttg cttttgactg ttctttcttt
     5941 tttaccagtc atgatttttg gtcttcagca aaacaccgca acagcagaaa aagaagcggt
     6001 actgcgtgtg gatggcacag aaatcaaaac attccctttg atggctggag aagagagcaa
     6061 cacatatact tacaaagatg aacacggaga ttataacttg atcgagatag atggcgatcg
     6121 agtacggata aaagaagcag attgcgatga ccagatctgc gtacgtcgag gctgggcaac
     6181 gaaaaatggc gaaaccatcg tctgtttgcc acataaatta gtgatcgaag tccgttcaac
     6241 agatggaggt gacgaagaca gtctgattta ttagaccttc acacaccatg agtaaattac
     6301 aaaaaatgat ttatatttcg ctacttgttg cacaaggagt gatcattggt ttattggaaa
     6361 atatgatccc ttatcctttt tcttttgcgc ctggtgcaaa actaggctta gccaatctaa
     6421 tcacgatcgt tgccattttt accatgaaaa aacgagacag cttcttacta ctttggctac
     6481 gactattcct gactactttg ctaggcggaa cgatttccac attcctttat agtatgagtg
     6541 gtgctttgct tagttatttt ggaatgttat tggtcaagca attaggacca aaacgtgtca
     6601 gtatcatcgg aattagtgcg gccggcggtt tcatgcataa tgtcggacag cttttgatgg
     6661 cttctttcat tgcacaatca tggacagtca tgttgtactt gccagtcctt tcagttttag
     6721 ggattttatc agggattgca atcgggatcg cagcgaatta tcttttacga catgtgcaga
     6781 cacttcaacg attccaaaaa gattatgagc agggaaccaa acaaaaatgg agcccaatgt
     6841 ttaccaaaaa ataatcgtca cacaaggagt ttttttatga agctacatcc aatgtgggaa
     6901 aactatcctg ctttaagtaa agaattgaca gaaacactaa aacggatgga agaaactgtc
     6961 cgcctgaaaa acaaacctgt cgaacaagca atcaaagaaa cgatccatgc cggcggaaag
     7021 ctgttgcgtc ctgcctatca gctattgttc tcacaatttg gaccagagca agatcgagac
     7081 aaagccatcg ctttggcagc ttctatcgaa atgctccata cggccacact gatccatgat
     7141 gatatcgttg atgaagcgga tctgcgacgt ggtcagccta atatccgtag tcagtttggt
     7201 aatgtcgttt ctgtctatgc aggagactat ttatttgtct gctgttttaa actattgtct
     7261 gattactcca cttccttgaa aagtctccaa ttgaattctc gcagtatgga aaaagtattg
     7321 aatggcgaat tagggcagat ggatgatcgc tataattatg aactttctgt aaaagaatat
     7381 ttggaaaata tttcaggtaa gacagctgaa ctgttccaat taagttgttc tgttggcgct
     7441 tttgaaagtg gaacaagcga gcgcttcgca aaaaaagcag gagatatcgg actttctatc
     7501 gggatggcct ttcagattat cgatgatatt ttagattata caaaagagag ccaggaaatc
     7561 ggcaaacctg tactagaaga catgcgtcaa ggggtctatt ctttaccgtt gatctactct
     7621 ttgcaaaaaa acaaaccaaa gctactacct tatttagaga agaaagcggc aatgacagaa
     7681 gaagatgtcg acgccgtacg caaaatcgtt gaacatacag gaggcgtgga agaagctagg
     7741 aagttagcgg cctcttacac agaaaaggct ttgaaggaaa taaagaaatt acctgccacc
     7801 tctcttcgaa caaaagagaa tctcttttcc cttacccaac tgattttgga tcgcaaagac
     7861 tgaataggga aaggttcgat acgtaaataa atttctcaga aggaaatcta cgtgtttttt
     7921 gacaaattga attgtagttc ttgtatttat gataaaaagc aaaaataaag agcaacgtcc
     7981 gttagcgacg ttgctctttt ttatcttgaa tattttttta ccttttcgat ttcttataac
     8041 tcataaaagt gattcttaaa ccgattacaa gtaatataac gcctgcgagt gttatccaat
     8101 tattcgattg acttccagtt ttaggaagtg ctctttttga tttgtttcca ttgtcattgc
     8161 ttgttttatt agttaagtta aagttcgtcc tgttatccga acttgttttt gagaaggaga
     8221 cacttgcatg acgtgtatcg cttgtggtag acgacgtgtc tgtactatcg cttgaagttg
     8281 atgaatctga gctatcactt gtggtagagg a
//
LOCUS       DACECR010000107         8264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.108, whole genome shotgun sequence.
ACCESSION   DACECR010000107 DACECR010000000
VERSION     DACECR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.108"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(84..722)
                     /locus_tag="IWO17_002413"
     CDS             complement(84..722)
                     /locus_tag="IWO17_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0291269.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(845..1477)
                     /locus_tag="IWO17_002414"
     CDS             complement(845..1477)
                     /locus_tag="IWO17_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0291270.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1828..2127
                     /locus_tag="IWO17_002415"
     CDS             1828..2127
                     /locus_tag="IWO17_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0291271.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            2622..4787
                     /gene="acm"
                     /locus_tag="IWO17_002416"
     CDS             2622..4787
                     /gene="acm"
                     /locus_tag="IWO17_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ0291272.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(5059..5544)
                     /locus_tag="IWO17_002417"
     CDS             complement(5059..5544)
                     /locus_tag="IWO17_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ0291273.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            6024..6665
                     /locus_tag="IWO17_002418"
     CDS             6024..6665
                     /locus_tag="IWO17_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0291274.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            6684..7319
                     /locus_tag="IWO17_002419"
     CDS             6684..7319
                     /locus_tag="IWO17_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0291275.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            7337..8152
                     /locus_tag="IWO17_002420"
     CDS             7337..8152
                     /locus_tag="IWO17_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0291276.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         2960 a         1263 c         1576 g         2465 t
ORIGIN      
        1 tccattgata catctgtctt tttgtttaaa ggaaataaag tatgctttct tagcaataga
       61 gcatgtatta tttctataaa aatttaggag aataaaacga ctagcaatat agctgtgaaa
      121 tcaaatatca catgaactgc ccaacttgta atgatcgagt tgcttttaat ggcagcttgg
      181 ttgaataaca atcttgccac cccttgtatc aaaaggatgt gtgctacagt gtgtaaaata
      241 tttccattat cataagtact gtaatggata aatccaaaga tgattgctga caagaaacta
      301 gcgacaggaa tgctgaactt actagaaaaa atagcgagaa aatacactga gaacaattct
      361 tctccaagta tcatgatagg caaaataata aataataacg ggctgaaagc ctcatttatt
      421 ggattgcctg ttaaacccca tttcaaaatt tgttgcaaaa ataccgagac tgcggcactg
      481 attactacat tgataaaaaa atatttggct atatttttga caggagcgac aggtttcgag
      541 aacatttttt tcgtatcttt tttaccaaaa acaagtgtaa atgctataaa tccagtgata
      601 gttaaagaaa aaatgctaat aatggatggc gcaaatagca accctaacaa accgagtaca
      661 acgacaatag tgcccaatat ccctttttta agttctattt tttttcctaa aatgttattc
      721 atgataataa ctcctactat tcagtttttg aagatagagt cagtatacat gaagagttaa
      781 aataggcaat gtttttttat ggatatttgt attgctaaaa atttatctta tatattcatt
      841 ttgattatac tacttttatt tgtaaaaatt ttctagcaaa accaagtgct tgcaactgat
      901 taagcgtaca attttttaga tgttccgagc gtacttcaat catttcaaag gagttgctac
      961 ttaaatcgat attttttaaa tcagtatcaa aaaagctagc attttctaaa tcattagtag
     1021 aaaacattat tttagtgagg gaacagtcta caaaatctgc gcttgataag acagagtgct
     1081 caaaggtact ttcttttatg tttgaaccag ataaatttac atagtccatt ttacaattct
     1141 cgaataacgt gttcccaata taactttcca tcaatttagt acctgttaat ttacaattga
     1201 caaatttcac gcgatgcaac atgctttttg tcaatataca gcctgaaaaa tcacagtttt
     1261 cgaatataca gtcggtaatc tccattcttt gaaaatcatt ggaagcaaaa aatgcatact
     1321 tgaaaataga tttgtaaata attggatggc agattatttg gttttcgatg acctcttttt
     1381 gtgaaaggat attatacagt tcaaaatctt cgttttcata gatatgaaaa aaggatcctt
     1441 tattcaagct tgcttcatta actttaggaa aaatcatatt taattacctc cattttcata
     1501 aagtcatatt aacaaaagct aaaccaactt acattagcag aaaatgagat aggttttcgc
     1561 ctttttcgat aacgatagtg tcgctatctc ttgaaacgtt cttagaattg ccctgagaga
     1621 cacaaattag gcgtaattct aataaaaatc ttagaataaa aatttttact gatttaattt
     1681 agaatgtttt taaaataaaa atcgttgcca taacagtgta ttgaaaggtt ttaacaatcc
     1741 agtaatgatt taaaaaaatc tagatgtagc gtatactttg tttatagaat gtagtgggaa
     1801 gaaatatatt tttgggagga aatcgtgatg tctaaaagtg aagaaataga aaacatggta
     1861 ctaaaattat atgatctcgt tttaaatcca gagatcaaag aaaaggaacg tatacttttg
     1921 atagacgcaa agacaggctt agaaaaaggt cagtattatc ctaaagtcat aaataatttg
     1981 gaacgtagtt tacgtccgtt agcaatcagg ggagaattgt ctaaaccagt atcaccgttt
     2041 tatatggaga tatcaacaat aggaaaattt gaaaaagaat taggtcgagg aatggcttcg
     2101 gcaccgatta cttttgggca tttataaaaa cgagttattc atctcaatga attaaaaagt
     2161 atagtcaatc tcataatgca ggctaggttc atttatttga acagagtctg tattttttta
     2221 ttctaaataa agaaacagat gtttctttat attgtatttt atcctttaat taacacaaaa
     2281 acagctagta taaactaata atgttaatta aaagtgtatt ttatgaaact atcgttattt
     2341 attaatgttg catttttgta agttaaaaat tggaaatata gattgaaaaa atgaaagcaa
     2401 taagtaaaat aaaatgaata tatgcgaaaa aataatttac attaggaaaa accttgtaaa
     2461 aacagttttt tggcatattt attttttata aatatttgta aaataactat caatttttta
     2521 gttaaaagat tggaaatgag tgggggagga attgaagaaa ggatttttga gagatgatat
     2581 agtaggtttt atttttagtt aaaatagggg agatagaaaa aatgaaaaag tgtagaaata
     2641 ttattttatc aatgttattt attattacta atataacatc actgattcct gttcatgtct
     2701 atgcggatgc aggcagagat atcagcagta atgtcacttc gttgaccgtg gacccaacga
     2761 atattacaga tggaggaaac ataaaagtaa aattctcgtt tgacgaaaaa aaacaaaata
     2821 tccaaccagg tgattatctt tggatcaatt ggccaagtga aggaaatatt cgcggtgaag
     2881 gattccaaaa agaaatccca ttaatgattg aaaataaaaa tgttggaact ttaacggtca
     2941 gaaaagattc tgcgcaggtt gtgtttaatg aaaatattaa aaacctagac tcagtagaag
     3001 gttgggggca atttgagata caggccagaa acgtaaccga taccggtgaa gaaaatacgg
     3061 gaccattcac cgtgacgagc ggtgataaaa cagctactgt aaatgtgaca aagccagctt
     3121 ctggttcttc ttctagcgtt ttttattata aaacaggaga catgctacct gaagatacta
     3181 agcacattcg atggtttttg aatattaaca ataacggcac ttatgttgaa caaccagtta
     3241 aaatcagtga tgaaatccaa agtggacaaa gattagatcc aagtacgttt gaaattaatc
     3301 aaatacactt aggtgaacag aaggtttata gaggagaaga gggaatccaa caatttttac
     3361 aagatttccc cagcgctaca tttaattttt cagtaactga taattacatt gaaataacta
     3421 taccaaaaaa ctttgtcaat cttaggaaaa taatggtgag ttataagacg atcatagaaa
     3481 acccagaaca aataaatttt gagaatcatt cggaagcttg gtttaaagaa tttaataaac
     3541 ctgctgttga tggagaaagt ttcaatcata cagttaaaaa tatttcagct tctggtggag
     3601 tcaacggtac agttagaggc gaattgaaga ttttcaaata tatcaatgac acagagattg
     3661 gtatcccaaa tgtgactttc gaattaagac gagcagatga acagcctatt caaggacagt
     3721 ctagtatcct gttgacttcg aatgaacagg gagaagctag tatcaaaggc ctgcaagtag
     3781 gagactatgt tgtaaaagaa aaagaagcac ccaattggat tgattttgat ccactaagtt
     3841 caaatgaact caaattctcc ataaacgaaa atgatacaga gggagtcagt ttacctattt
     3901 ataacaagaa aaaggttacg aacatcacag caactaaaat ttggaacggt ggaaccactc
     3961 caagaccgag tatttatttt aaattattca gagcaagtac aaacaattgg gaaccagttc
     4021 ctgatgcaga aacaaaacga ttagacaatg gaataacttc agtgacttgg gaagatatcc
     4081 aacagtatga tgatagtgga aacgaatata cgtttaaagt gcaagaagta gatcaaaacg
     4141 gaaatgatta tgtcccttca ggttatcaaa aaattgaaaa cgggctagtc gttacaaatg
     4201 agaataaaga agtcatttca gtctcaggac aaaaaacatg ggaagacaat gagaaccaag
     4261 acggcaaacg accgacgacg atcacagtaa atctattagc cgacggtcaa ccgatccaac
     4321 acaaagaagt aagtgaaaaa gatgattggc gctatcgttt cacaaatctg ccaaaataca
     4381 gagacgggca agaaatcatc tatactgtaa cggaagacaa cgtgcccgaa tacagtacga
     4441 cgattgacgg gtataacatc aaaaacagtt atacaccgga aaaaacaaat atatctatac
     4501 caggtagcca tataacaccg tggaaaaatt ttccagagaa gacagagaga gagaaaatta
     4561 gaaatctatt ctctcaaaaa ttgcaaatat ttgctcatac aggagaatcc agcgaacaaa
     4621 gaagcaatta tattagtaag agagctgtac ctaaacagat aacaaataaa tcaaaaagca
     4681 ttcttcctaa aacaagtagt aaaaaatcta gttttgctgt aatcattggt ttattgatag
     4741 taactatctg tgctgggata ttcttacaaa aatataaaaa agtttagtta gaatataata
     4801 ttttttgtta aatatgactg aaatcaaaag aataaaattc cgatttataa tcgaaaaaag
     4861 aagcattttt atatatgctt cttttttcgt agaatcaata aatatggatt tggctaacta
     4921 tggaagaata ctcgtattag ttttcagcgt tcatactagt tgttacattg tttgattggc
     4981 caaaagaatt tctgatatat tttgtgctaa ataaagaaaa agacgcttga attgggaaat
     5041 aagaaaacta ctctatcatt acaaattttc ataaactggc agatgttcta agtaaacaag
     5101 tatcttttca aagtcggaaa aatccataat caacgtagtg gtattgatat ttggatgaaa
     5161 gcctacggta tcttgacgat caatagcagc atcgatcacg acttggattt ttttctcttt
     5221 gtcgtgcatc aatgctaatg gagtcactgt acctgctgga acgcctagga ggtcaggaag
     5281 ctgttcttca gaaacaaaag ataagcgctt ttcttttaat tgttcagcta gttgttttaa
     5341 atcggcgtgc ttttcatcag gcaagatgac aagatagatt tgttttcctt ttcttgattt
     5401 caataataga ttttttactt gcggaccagg tagggaaaaa gggacatttt tgactgacgt
     5461 aatcgcagga tgatcgactt tttgaaaaga gatacccagt ttttttagta gttcataagc
     5521 ttcttgttcg gttgcacgac tcatcgcgca tcaccctcct ttttttatta gtgtatcata
     5581 aaaaagagtt gacaaatgaa aaataatcat gtaaattaca cgtaaagtta aaacaaatga
     5641 catcgaaaag aagagtagcc gttaggtgtt tttagagagt ctctggttgg tggaaagagg
     5701 cagcagttaa ccgtgaaaca catcttggag tcgattttct gaagcgtata gtaggaaaat
     5761 ccggggagcc cgttacagct gcggtcgttg ctagatgacc agataaggct ggcttttccg
     5821 tgaggagtcg gtaaaaaaag gtggaaccac gtgattaacg tcctttgatc ttcggatcaa
     5881 aggacttttt gttttcatct gaacggcaac gactgtgtga ggttttttct gcaaggaaaa
     5941 aataaacaga ggtggaacca cgtataatac gtcctcttgg catttgccaa gaggattttt
     6001 ttattttaaa ggaggaaaag aagatggagt atttgattga agttatgccg caattattag
     6061 taggagcact cacaacattg aaattatttt tatttacctt gattggctca ttaccattag
     6121 ggattgtttt agcattagga ttgtctttgc attttaagcc gctacgctac ttgcttcaaa
     6181 catatgtatg ggttatgcgg ggaacaccgc tgttgttgca actgattttt attttttatg
     6241 ggttgccgac gattgggatc gtttttgatc gtttcgaagc agcactcatt gcctttatct
     6301 taaactatgc agcttatttt gcagagatct ttcgtggagg gatacaatcg attcctcaag
     6361 gtcaatatga agcagcgcaa gttttgcaat taagtccctt tcaaacggtg acgaaaatcg
     6421 tcatcccaca agtgacaaag attgttttac cttctgtagg gaatgaagtt atcaacttgg
     6481 tcaaagattc ttccttgatc tatatattag gtttaggtga cgtaatgcgt gctgggaaaa
     6541 tcgcgatgga acgcgacgcg actttactgc ctttgctagg cgtggcaatc atctatctta
     6601 cattgactgg tattctaaca atcataatga aacaagttga aggaaaatta aattattacc
     6661 gctaactgat aggaggaaga aatatgattg aattgaagaa agtgaacaaa gcatttggtg
     6721 aaaaaaaaat aatcaaagat ttgaatctaa tcattccaga tgggaaaaca ttagctatcg
     6781 taggtccttc cggtggcggt aaaaccacgc ttttacgtat cttggctggc ttagaaagtt
     6841 ctgacagcgg agaattttat ttagacggtg taccgttcaa cccattcgag cttaaaatga
     6901 aagaacaagt tgtaggagtc gtattccaag acttccagct atttccacat ctgaatattt
     6961 ttgaaaacat taccttagca ccaaaaatgg tattgaaaca gacaaaagaa gaatacacca
     7021 aaaaagcaga agaattagcg gcttcattag ggattagtga tttattgaat atctatcctt
     7081 atcagctttc tggcggacaa aaacagcggg tagcgattgc cagagcattg gctatgaatc
     7141 cacgggtatt ggcttatgat gaaccaacaa gtgctttaga tcctgaatta cgccagcaag
     7201 tcgagcgttt gatcctatca ttgaaagaag atggggtaac ccaaattatt gtcacccatg
     7261 atttagtatt tgcagaaaat gtcggtgatc aattactaaa agtttcccca attcattaag
     7321 aaaggagaag atagagatga aaaaaaccag ttttctattc gctttgatcg caggtcttct
     7381 tctttttgca ggctgttcga acaaaaaaac ttcagcagat aattgggcga ctatcaacga
     7441 agagaaaaaa atcgttatag gtttagacga tacatttgtt cctatgggat ttagagataa
     7501 agacgggaag ctcacaggat ttgatattga tttagccaaa gcagtctttg aacaatatgg
     7561 gattacggta gactttcagc ccattgactg gtcaatgaag gaatttgaac tgaacaatgg
     7621 aacgatcgat ttgatatgga acggctattc caaaacgccg gcaagagaaa agaaagtgca
     7681 atttacaaaa ccttatatgg aaaacgatca agtgctgatc acacctaaag aatcaggaat
     7741 cacaaacttt ggacaaatga ataataagag attaggtgcg caaaatgggt catctggtta
     7801 tgatgtattt acgaaacaac cagaagtatt aaaagagata gtcaaaaacc aagatgctgt
     7861 tttgtatgat agttttaatg aagcattgat cgatctgaaa agcagacggg tcgatggtct
     7921 gctgatggat aaagtatacg cagactatta tttgaaacaa agaaacgaac tagaagactt
     7981 caacataaca aaaggttctt atgagagtga ggattttgca gttggtgcac gcaagtcaga
     8041 tagtacatta gtgaaaaaaa tcaatgaagc cctagatcaa ctagagaaaa caggtgaatt
     8101 ccaaaaaata tccgataaat ggtttggcga agatgttacg ccgagaaatt aacaaatagg
     8161 ggactgtgac aaaagacgtg tcacagtccc tttttccgaa taaacagtgt tctcctgcca
     8221 actccttgtg actcggagct gaatggcagg ctcacaacct cttg
//
LOCUS       DACECR010000108         8134 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.109, whole genome shotgun sequence.
ACCESSION   DACECR010000108 DACECR010000000
VERSION     DACECR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.109"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(322..1461)
                     /locus_tag="IWO17_002421"
     CDS             complement(322..1461)
                     /locus_tag="IWO17_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0291277.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(1575..2159)
                     /locus_tag="IWO17_002422"
     CDS             complement(1575..2159)
                     /locus_tag="IWO17_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAQ0291278.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(2286..2717)
                     /locus_tag="IWO17_002423"
     CDS             complement(2286..2717)
                     /locus_tag="IWO17_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0291279.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(2735..3055)
                     /locus_tag="IWO17_002424"
     CDS             complement(2735..3055)
                     /locus_tag="IWO17_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0291280.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            3787..4044
                     /locus_tag="IWO17_002425"
     CDS             3787..4044
                     /locus_tag="IWO17_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAQ0291281.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(4015..4323)
                     /locus_tag="IWO17_002426"
     CDS             complement(4015..4323)
                     /locus_tag="IWO17_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291282.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            4401..5174
                     /locus_tag="IWO17_002427"
     CDS             4401..5174
                     /locus_tag="IWO17_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ0291283.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIRK
                     HGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSST
                     DSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPKA
                     DGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            5189..5392
                     /locus_tag="IWO17_002428"
     CDS             5189..5392
                     /locus_tag="IWO17_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0291284.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            5408..5728
                     /locus_tag="IWO17_002429"
     CDS             5408..5728
                     /locus_tag="IWO17_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ0291285.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            complement(5910..6692)
                     /locus_tag="IWO17_002430"
     CDS             complement(5910..6692)
                     /locus_tag="IWO17_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291286.1"
                     /translation="MDISDTPYYQKLIASSKLIGETIAPSLDVVNAMQPAIEQAKKLI
                     AIQDTFIASQAILNDSLIQIVEKRNKSIANMIPPSLFAIQKQLESPSFNIAKKMAATM
                     PKPYFSDISKITERFAVDMSKYDFRIGVATQELEKAFSSEPEVSTKNLLFKDTSNPSD
                     EFEAKFFDILNEQRKVLDDSIKLFEEINTAKNKDITNEPSEKQQASPAFYKDKMWYLE
                     QTGAALIGLVVTEIVNITIGVDPNNTISLALFLRYLLQFLIN"
     gene            6743..7450
                     /locus_tag="IWO17_002431"
     CDS             6743..7450
                     /locus_tag="IWO17_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ0291287.1"
                     /translation="MQELVILKNKEAVTTSLQVAETFRKEHRAVLKAIDDLKDGLAQK
                     YADLFWEDVYIHPQNKQAYRIVYMNRDGFTLAVLGFNNTQKVLEFKLKYIEAFNEMEK
                     AIRKNTAFDTIEDMMIYQLEEMKDVKKDVSMLKNTMRISGQQEFEIKQKGNTKVMEVL
                     GGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRM
                     EIDQLNRQQRLFGDENE"
     gene            7628..7969
                     /locus_tag="IWO17_002432"
     CDS             7628..7969
                     /locus_tag="IWO17_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291288.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            7962..>8134
                     /locus_tag="IWO17_002433"
     CDS             7962..>8134
                     /locus_tag="IWO17_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ0291289.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHY"
BASE COUNT         2807 a         1326 c         1397 g         2604 t
ORIGIN      
        1 tcaaatttaa accataattg tgtttttcct tccatcagca taaaagttca cctcaaaaag
       61 agtatacgaa cgaatgttct ttttgtgaag gcggaattta atatactgaa taatagaaaa
      121 caagtacttg tgccaaatta tgtgccaaaa aaattcgaat tcaacaaaat gtaaacaaat
      181 agaaaacctt ctatattaag atttcctgca ataacaaaca caacagaaga cgtgtaatag
      241 ttagtcagga acgtacaaat aacccctgta tcctttgtgg tacaggggtt tgttttgtat
      301 ttgtgccaaa aatgtgccaa actagttatc atttttatat aaactatcga acacagtatt
      361 gattttttct ttatcctgat cttcaagctc tcgaacaata tgaagataag tagacattgt
      421 agtttctaaa ctgctatgtc ctaaacgttt cgatacgcta agtatattta ctccttgata
      481 aagtaaaata gatgcatgtg tatgccttag tccatgcaaa gtaaattgct tttcaatccc
      541 taattctttt aattttttgc gcaaatattt gctgacggcg tttgatgaaa ctaatccatt
      601 ttttaagttg aaaaaaacaa aattatgagg gtttttaact tcaaaattct cgaataattc
      661 tttttgattg attttaaact tttttaagag atcaatcgta tgtttatcaa tggaaatttt
      721 cctatttgaa gtttcgtttt ttgtttttcc ccattcattt aatttataat tccatgtttt
      781 attaatagtg atgatttgat cttcgaaatc gatatcttcc catgttaatc ctagaagttc
      841 agcaaatcgc attccagtag caccagcaac taacacgagc ataggagaag agtactgcgc
      901 agaaaggttt tcttctacga gtttcattaa agatttaaat tgatcataat ctaaatattt
      961 atcttctttt ggcttcaatg aatcttttcc tttgattact gcttttctag ttggatcaaa
     1021 cggaattaat ccttcttcta cggcatcttt caacgatgct ctaatatggt tattaaactt
     1081 aataacggtg gattttacat ggtctttcgc atacttattt aaaaagcgtt gatatccaac
     1141 tctatctaaa tcagaaatca ataccgctgg catatatttc tttatgttca ttaacgtatc
     1201 ttcgtatttt ctataagtga taggagatac tgttccttct ttataaagtt gcatccaatc
     1261 ctcaaagtaa tctgataata atagattttt gcgatcccct ttaaggcctt tgttcaagtt
     1321 atattctaat tcgttggcgg catcttttgc ttcgcctttt gtcttaaatc ctgattttct
     1381 tatcttcgtg tattttccgt catctttttt gtatgaaatt tcgtattgcc aactatttcc
     1441 acgtttgact aatcttgcca taattgaatc tactctcttt ctctgataca ataggcacta
     1501 taaagaagcc tactgtatag gtttgttttt cttagaacac gcttgctttg gacggtgggc
     1561 gtgtttttta tttattatac agttgcttta aaaatagctg ctttttcaaa tgatactaaa
     1621 ggagagaagt ggatttctat ttctcccttt tggtttaacg tgaaatgagc agttacatcc
     1681 atttgttttc ctggagcaac agatcccatt gtattttcgt tggcatatgt ctctgatttt
     1741 ttgtcatctg gtccatatac ttccacatct gtacctacag ggatatctga atcgccatca
     1801 ttttttacgg tataagtaat ttttactact tgtgcaggtt ggttttcttc aaattgattt
     1861 ctttcatcag ttagttctac actatttagt gtatattcag catcaccaac ttttacagtg
     1921 tcaccaatct tatagaaggt atcgcttttt tcttctttag aagatgaagc agaggttgaa
     1981 gatttagtta ctttttcgcc accattatca cttgctttat tactatctga tccaccattt
     2041 aaagcagaac caataattat aattaaaatt actgctaata cccaaaacca tacttttttg
     2101 taaaaaggtt tactaacttt atacattttt ccgtcttgac ccataacttt ttttgccatt
     2161 taaatattcc tcacttcttg ttataatata tttgcgatct cagaaatgag gtatgagtcc
     2221 gtgtgccagc acgggctttt ttaatgtttt gctgaaatcg gtttttttaa taattcttga
     2281 tgtattcata catattgcct tgcgtaagaa tattttttct taaaatagca ttggcagaca
     2341 gcataacaag tgtatctgag cttattctga atagaataat attccataaa tttttcgagg
     2401 ttaaactgag attcatcagt cagttcgttc tcaatataga tatttaataa aattagaata
     2461 gctattttat ctgcttctgt ttcaaatttt gagtgaaaag ttgtagaagt atcgtacaaa
     2521 actgaaaatt caaaatgaga ggcgctgaaa tgtgcaagtt catgagataa atgaaaggct
     2581 tctgcagttt cgccatatag attttcattc aaaaaaatga ttctaggctt cggataatag
     2641 aagccaggct ctttcatttc catgtaaact actttcaaat tgtattcgct caacatttct
     2701 ttcaatttca aatacataca acccatcact ccaactattc attttcctct aaagctttag
     2761 caattgcaat cgctttacgc attgtctcct tagatatttc ttttccatca aaagaaaaaa
     2821 cagtatcgtt ttctgataaa tccacatgtt taggggtttc tttttcttcg cgccccagaa
     2881 gatagtctac agagacatcg aaatagtcag caatttcttg caaacgttct gtagaggggt
     2941 ttgagttctt aagtctatat aagacgtttt taccatagcc taggtcttct tcaactttat
     3001 ttaatgattt tccctgtttt ttcgctaact cttttattct ctcaaacgtt atcatatcaa
     3061 tattcctttc gttcaagaaa aaatatttaa ctttatttgt taaaattcgt tgactatttt
     3121 aacaaaaggt gttaatatca ttcttgtaaa caagtttaac aactaaaaag acaacaaaaa
     3181 acactattga ttaataaatg ccaaccgcca agaaagcttt taaaatcaat gtttatatgt
     3241 cttatttaac tatgttctga ttttaacact gtatgttaat ttagtcaacg cagtttttta
     3301 aaaagttgtt aaatttgttt acgaatataa aagaaaggag agaagaatat ggaaaaaaca
     3361 gtctcggcaa aaatcgaaga tttgaaatta gacatactga aacaagcaaa agtggcgatg
     3421 gaacacgcgg tagataaaga agactctgcc atggttgcag ccatagcaga gattttagct
     3481 cacgtttagt catcgttttt acggtcttct ggaagctgac catagataac agcataatga
     3541 atatctaaat aagcttgaac aatttctttt ggcgaaattg attcgccttt agtagatact
     3601 tgtgattcgt gatttagcca tgcaacaact aaatccgcag cgatttcaaa cggcgttaca
     3661 ctttgtgtca gttgccataa agaatttcac aatatgtatg gtcgtggtaa taataccaaa
     3721 gagcaatttg aggaatttat ggaaattaaa aaatacaaaa aatcatttat taaaggaggc
     3781 taaacaatga acaaggaaat gagccaaaca atcaaggtac aaaaaatgat agatgatcta
     3841 acacatggaa ttgatagcca agccgataaa atcatcaagg aattgcaagg acaaaaagtt
     3901 aaggatgcaa aaatgcttct caagactatc aactttgaaa tgaatccaac aaaaagaaag
     3961 ctcgccgatg tgttggaaga aaagttggct tccgctatca acgagcaaga attactattt
     4021 gaaaccgata cttttaacag ctgatgtttt atagatgaca gtttcatccg aatctatatc
     4081 atcgccgaga gaaaagaatg ttgcttttga caaaaagccg ataagaccta caagcggtct
     4141 tgatgttgca agagtagata ccccaatatc accttgcaag gtattcacgt aaatttgaga
     4201 agaatagaac attccatctt tttcttcgat atttgacgca tcatcgtatc caacgaccaa
     4261 agtatcggca aatattttga ttttttctcc atcaagcata gtaattacaa attctggttt
     4321 cataaatttt cacctcctta acaattattt cagcctgtca cactgataag gaaattatac
     4381 caaagaaagg aatgagaaat atgaatacac cacaaatttt taatttcgag caaaacgaag
     4441 ttcgaactat tttagtaaat gatgaaccat attttgtagg aaaagatgta gcagacgttc
     4501 taggatattc gaatccgcaa aaagctattc gagatcatgt ggacttggag gataagacgc
     4561 agaacgattc gttcaccgtc aatggaacag cagttgtttt gattaacgag tcaggccttt
     4621 acagtttaat cttaaaatca aaacttccct ctgccaaaaa atttaaacgt tgggtaacaa
     4681 gtgaagtgtt gccagcaatt agaaaacatg gaggttatct aactccagaa aaagtagaag
     4741 aagctttgct taatccagat acaatcattc aattagcaac tcaactaaaa gaagaaagaa
     4801 ctggaagatt aatcgcagaa caaaagattg cagagtacga acctaaaatc tcctatttag
     4861 atagcatatt atcttctaca gattcagtaa caattagtca gattgcagca gattatggga
     4921 tgtctccaca acagatgaat aaattacttc ataaactagg tattcagaaa aaagtcggta
     4981 accaatggtt attgtgcaaa aaacacatga accaaggata cacaaaatct catacaactg
     5041 agatcccgaa agccgatggt ggcactaaaa ttgtaatgaa taccaaatgg acacagaaag
     5101 ggcgtctatt tatctacgaa ttactaaaaa aagaaggata ttaccctcaa atggatttag
     5161 aggaaattgg ttagaaagga gttttagtat gactgacatt gcagaaatca ctcaacgaga
     5221 tagagaaaaa atcaaagaat atgtcgaaag ttcaaagttc ttaacttaca ccatgctcgc
     5281 tgaaagattc ggcattagca aaagctactt atctttaatt ttaaacggta aaaagacttc
     5341 tgcagaagca aatagaatca tagattcgat tattactatg tacgaattgt aaagggagga
     5401 acagctaatg caatatctag aagcgaaaat cccagttcca gaaggctatg taattatctc
     5461 ccaagtggat tatgaggagt taaaaaaagc tgatgatact ggtagatgga tgacgttgcc
     5521 agaagtgcta gaacggatta acagaaaata tgattggttt acttctagag ttttaaagaa
     5581 cccaagatat agaaatatta tcgatatcga aaaaaacaaa aatggatttg tctattatcc
     5641 agttgaagga agagacacat atctattttt aagaagtaaa acacttgaat ttttagaaac
     5701 aaatttttcg gaaatcttaa ggaggtaagc ggatgggtaa atttaataga gcactagtat
     5761 tcagcgcacc actaattatc tacgctttag gtctttgggg aagcagacaa gcattaatcg
     5821 gcacaattgt atatatggtc tggatcttta tggggcttga tgaagctgaa tatagagcga
     5881 aaaagccagt cgggagggac tgacttaacc taattaatta aaaattgtaa caaataacgc
     5941 aaaaataaag ctaatgaaat tgtattgtta ggatctactc caatagttat atttacaatt
     6001 tcggttacta ctagtcctat caaagctgcc ccagtttgct ctaaatacca cattttatct
     6061 ttgtagaaag caggtgaagc ttgttgtttt tcagatggtt cattagttat atctttgttc
     6121 ttggctgtat ttatttcttc aaaaagcttt atagaatcat caaggacttt cctttgctca
     6181 ttaaggatat caaaaaactt agcctcaaac tcatcagatg gattagaagt gtctttaaaa
     6241 agtagatttt ttgtactaac ttccggttcg gaagaaaatg ctttttctaa ttcttgggta
     6301 gctaccccta ttctgaagtc gtacttagac atgtcaacgg caaatctttc tgtgatttta
     6361 gagatatcag aaaaataagg ttttggcata gtagctgcca tttttttagc aatattaaaa
     6421 cttggagatt ctaattgttt ctgtattgcg aacaaagaag gaggaatcat attagcaata
     6481 gatttgttac gtttctcgac aatttgaatg agagaatcat tgagtattgc ttgcgaagct
     6541 ataaaagtat cttgaattgc aattaatttt ttagcttgtt ctatcgcagg ctgcatagcg
     6601 ttaacgacat ctagagaagg agcaatggtt tcaccgatta attttgaaga agctatcaat
     6661 ttttgataat aaggagtatc tgaaatatcc aattatatac accacctttt tacctaaatt
     6721 ataccaaaag ggagagaaaa aaatgcaaga attagtaatt ttgaaaaata aagaagctgt
     6781 gactacgagc ttacaagtcg cagaaacatt ccgaaaggaa catagagcag ttttaaaggc
     6841 gattgacgat ctaaaagatg ggcttgcaca aaagtatgca gacctatttt gggaagatgt
     6901 gtacatccat ccgcaaaaca aacaggctta tcgaattgtc tatatgaatc gtgatggctt
     6961 tactcttgct gtattaggat tcaacaatac tcaaaaagtt ttagagttca aattgaaata
     7021 cattgaagcg ttcaacgaaa tggaaaaggc aattcgcaaa aatacagctt ttgacacaat
     7081 tgaagacatg atgatctatc aattagaaga aatgaaagat gttaaaaaag atgtttccat
     7141 gcttaaaaat actatgcgaa ttagcggaca acaagagttt gaaattaagc aaaaaggaaa
     7201 tacgaaagtt atggaagttc tagggggaaa agaaagccga gcttatgaag aaatcagcaa
     7261 aaaagtattc tcaaaatttt ggtctgaatt taaacgtacc ttttcaatcc caagatatgg
     7321 cgagttacct cgtaagagat tcgatgatgc tgtttcattt attgaaatgt ggttaccaga
     7381 aactgcgatt cgcatggaaa tcgatcaact gaacagacaa caaagacttt ttggtgatga
     7441 aaatgaatag agctgaagcg ctaagaatcg ggacggtaat tgctaatcgc tggtggagac
     7501 acaataaacc aatcatccta agccaacaac atattgacaa gcaaaaagca tggcaacaaa
     7561 taaaaaagtg actccgccgg caagcaaaga gtcacaaaga aaacacatca taaggagatt
     7621 ttagcatatg gaaaaagaac tttccactct agatcaatat ttgactgatc ctagttgggg
     7681 caaatcgaat atcaaggaaa caaataatcg aaaaatcaga cgaaatcttt tgacggatga
     7741 agaactagca tgtgatcaag acgatttggg aaattttgtg agtatttggg atcatgtcta
     7801 tcttatccat ttatcgagga agtccaaaaa acctgaatat atctatgtca tcgaagatgg
     7861 cttgattgat gcgctagaag agtatgacag agataacttg attgatatct cttattacgg
     7921 atcaggtaag aaatacattg ctgaaatgga ggcagaattt gatgagtgaa aacaaaggga
     7981 caacgacttt tgaaaaactt tttagtcgca agttaaataa aatactcaag aaaaaaggaa
     8041 attttgatta tttatcttgg gctcacgcat gggagattat gaaaaagaat gatccacagg
     8101 caacggtaac tattaatgag tataaacact acag
//
LOCUS       DACECR010000109         8097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.110, whole genome shotgun sequence.
ACCESSION   DACECR010000109 DACECR010000000
VERSION     DACECR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.110"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(310..807)
                     /locus_tag="IWO17_002434"
     CDS             complement(310..807)
                     /locus_tag="IWO17_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0291290.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(847..1656)
                     /locus_tag="IWO17_002435"
     CDS             complement(847..1656)
                     /locus_tag="IWO17_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0291291.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1990..2847
                     /locus_tag="IWO17_002436"
     CDS             1990..2847
                     /locus_tag="IWO17_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0291292.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2840..3742
                     /locus_tag="IWO17_002437"
     CDS             2840..3742
                     /locus_tag="IWO17_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0291293.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3775..4125
                     /locus_tag="IWO17_002438"
     CDS             3775..4125
                     /locus_tag="IWO17_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0291294.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4159..5097
                     /locus_tag="IWO17_002439"
     CDS             4159..5097
                     /locus_tag="IWO17_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0291295.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5175..6068
                     /locus_tag="IWO17_002440"
     CDS             5175..6068
                     /locus_tag="IWO17_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0291296.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6106..6669
                     /gene="amaP"
                     /locus_tag="IWO17_002441"
     CDS             6106..6669
                     /gene="amaP"
                     /locus_tag="IWO17_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0291297.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6683..6871
                     /locus_tag="IWO17_002442"
     CDS             6683..6871
                     /locus_tag="IWO17_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0291298.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6884..7786
                     /locus_tag="IWO17_002443"
     CDS             6884..7786
                     /locus_tag="IWO17_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0291299.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7818..8060
                     /locus_tag="IWO17_002444"
     CDS             7818..8060
                     /locus_tag="IWO17_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0291300.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
BASE COUNT         2804 a         1414 c         1586 g         2293 t
ORIGIN      
        1 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
       61 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
      121 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
      181 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
      241 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
      301 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
      361 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
      421 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
      481 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
      541 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
      601 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
      661 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
      721 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
      781 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
      841 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
      901 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
      961 gctgtaattc aacatagcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     1021 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     1081 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     1141 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     1201 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     1261 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     1321 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     1381 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     1441 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     1501 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     1561 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     1621 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     1681 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     1741 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     1801 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     1861 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     1921 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     1981 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     2041 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     2101 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     2161 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     2221 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     2281 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     2341 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     2401 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     2461 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     2521 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     2581 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     2641 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     2701 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     2761 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     2821 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     2881 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     2941 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     3001 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     3061 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     3121 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     3181 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     3241 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     3301 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     3361 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     3421 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     3481 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     3541 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     3601 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     3661 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     3721 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     3781 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     3841 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     3901 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     3961 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     4021 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     4081 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     4141 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     4201 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     4261 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     4321 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     4381 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     4441 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     4501 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     4561 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     4621 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     4681 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     4741 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     4801 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     4861 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     4921 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     4981 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     5041 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     5101 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     5161 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     5221 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     5281 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     5341 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     5401 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     5461 acgtgaaagt tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     5521 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     5581 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     5641 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     5701 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     5761 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     5821 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     5881 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     5941 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     6001 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     6061 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     6121 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     6181 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
     6241 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
     6301 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
     6361 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
     6421 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
     6481 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
     6541 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
     6601 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
     6661 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
     6721 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
     6781 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
     6841 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
     6901 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
     6961 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
     7021 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
     7081 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
     7141 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
     7201 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
     7261 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
     7321 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
     7381 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
     7441 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
     7501 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
     7561 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
     7621 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
     7681 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
     7741 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
     7801 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
     7861 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
     7921 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
     7981 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
     8041 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttct
//
LOCUS       DACECR010000110         7980 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.111, whole genome shotgun sequence.
ACCESSION   DACECR010000110 DACECR010000000
VERSION     DACECR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.111"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="IWO17_002445"
     CDS             complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="IWO17_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0291301.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1229..2332
                     /locus_tag="IWO17_002446"
     CDS             1229..2332
                     /locus_tag="IWO17_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0291302.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2525..3592)
                     /gene="rlmN"
                     /locus_tag="IWO17_002447"
     CDS             complement(2525..3592)
                     /gene="rlmN"
                     /locus_tag="IWO17_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0291303.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3822..6269)
                     /locus_tag="IWO17_002448"
     CDS             complement(3822..6269)
                     /locus_tag="IWO17_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0291304.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6458..7894)
                     /locus_tag="IWO17_002449"
     CDS             complement(6458..7894)
                     /locus_tag="IWO17_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0291305.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2214 a         1736 c         1359 g         2671 t
ORIGIN      
        1 ggtacacggt gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca
       61 agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct
      121 ctttttatta ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt
      181 tgtcccagcc atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga
      241 attcatacgg atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat
      301 acaggtccgt ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg
      361 tgattcctcc tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt
      421 ggttctttcg tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt
      481 attgagggat tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga
      541 ttcgttctgg attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg
      601 gccaagtcag tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa
      661 attcactgat tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa
      721 aaaacgaaat gttctcgctc aaaaatcttt agctaccaat cagtatctct attctttcat
      781 tatttatctt acttataaaa taaaaaaatt atacagaaac actttattca aaataggaaa
      841 gatcaggaaa gccatcaatt ggaggaaaaa actctgtttg ttcatcatga tatagattat
      901 cttttgcttt aaaaaaaacc aattatgaat tgacaaataa aacctgtttt catataataa
      961 tcttaatttt tagaattttt agataattac aaaaaagcaa agggaatcgg tagaaatact
     1021 aagaggaaca aaaattcatt attgtcgttt tcaatgtttt tgtacttgtt cgtttttagg
     1081 atacttattg atgatttcaa ggtgttcatg ccaccttatt catcacttat cctgtcttag
     1141 gttcacctct actccatcta cagccgcagt cagatcgatc actctttaaa attttaaccc
     1201 gtttcaacga agaaagaagg aaatgaatat gagtcaaagt gttttcttac caaattatac
     1261 cattggaaca gatgcgtatg aaaaagcaaa tggaatcgtc aaagaatatg ggacaaaagt
     1321 agtgctgatt ggagggaaga ctgcactttc aaaggcagaa aacaaaataa aagtagcttt
     1381 gacacaggaa atagaggttg tcgccagtct ctggtacggg ggtaattcaa cttatggaaa
     1441 tattgaacag ttgatccagc atgaaagtgt ccaaaaagca gacgtcctgt ttgcagtcgg
     1501 tggtggccgt gcttgcgata ctgtaaaagt cattggtgaa aaactagcta aaccagtctt
     1561 cacctttccc actattggtt cgaactgttc gcccgtcact gcggtatgtg tcatctataa
     1621 cgaagaaggc gtaatggacg gactcttctt tccttcaaaa ccacctgtac acaccttcat
     1681 tgatacagca attattgccg aagcacctga taaatactta tgggcaggta ttggcgatgc
     1741 tttatccaaa gaagtcgaat ctactttttc tgctcgtggc gatgatctag gctacacgga
     1801 tagattagga atccaaatcg gaaaaatgtg cattgatcgt ctcttgactt atggtacgca
     1861 agcattgaaa gatagtgccg acgatcgtcc ctcatatgca attgaacaag tcgtttcgga
     1921 tatcattgtt actaccggat tggtttctgt tttagttaac aataactata attctgctat
     1981 ggcacactca ttctattacg gtacgactgt tctagaaaaa atggagggat accttcacgg
     2041 agcatctgtc agttatggtg tactgacctt attgatgatg gatcagcagt tcgatttgtt
     2101 tgataaaatc tatgtgttta tgaaaaagaa ccaacttcca gtatcgttaa ccgacatgaa
     2161 tctcaatgtc acgaccgatt tagaagcaat cttagataaa gccatggcaa caaatgatat
     2221 taaacatgtg ccttatgaaa tcacccgaga aaaatttaaa caggccattc ttgacgtaga
     2281 aacttatcat gctacacaag aaagccagca aggatataag aaaatcagtt aaataattgc
     2341 taaaaccccc atcaagcagg ctgtcatcaa attgaggaca tttctttgct tgatgagggt
     2401 ttttcttgtc aaaaaaagct ttatagcttt cgctatagaa tcttttctct tatttaagtt
     2461 gaacagttcc ttaattaaga aaactgctcc ttttatggta attgaaaaaa cgagtaggat
     2521 acgattattt ggcaactgtt tttttcattt gcttgcttcg caattgtccg caagctgcat
     2581 caatatctgt accatgttct ttacgtatca cacagtttac gccgtttttc ttcaatacat
     2641 cataaaatct caatacatca gcttttggac tgcgagaata ttgatcatgt tcactgactg
     2701 gattatatgg aatcaagttg acatatgcta attttttctt atcttttaat agatctgcta
     2761 attgttgcgc atgctctggt ctatcattta cttgattcaa catgatatat tcaaacgtga
     2821 ttcgacggtt tgttttctct aaatactcat ccacagcttc catcaatttc tcaattggga
     2881 aacttcggtt gatccgcatg atcgatgttc gcacctcatt attcggcgcg tgcaaggaaa
     2941 tagccagatt gacttgcaag ccgttattag cgaattcctt gattttgtgt gctaatccac
     3001 tagtggaaac tgtaatgtgc cgagcaccaa ttgccaatcc tttggcatca ttgatgatat
     3061 gcaaaaagtc cattacattg tcgtaattat caaatggctc accgatcccc attacaacga
     3121 catgtgaaac gcgttcgcca agattgcgct catcaaaata atgttggacc atcatgatct
     3181 gtgcaacaat ttctccagca gtcagatcac ggttcttttt gagcagcccg cttgcacaaa
     3241 acgtacagcc gatattacat cctacttgtg tcgtcacaca aactgacaag ccatattctt
     3301 gtctcatcag tacagtctca atcatgtttt tatcagggag ttcaaacaaa tattttactg
     3361 taccatccga agcttcttgg acgaccatct gttttagagg attgatgata aaatgctccg
     3421 ataatttttc gatcaattgt ttcgatagat tcgtcatttc tgtgaaatta cttacacgtt
     3481 tttgatacaa ccattcccaa acttgggcag ctcggaattt tttctcgccg ttttctataa
     3541 accaattgat taattcttca ttcgtcaagc cataaatgga ctgtttttcc aaagtatttt
     3601 ccctcttttt ctcaaagttt cgctacatac tatacatgat tcatttcctt aatgcaatac
     3661 ttcacaaata aatcgaatag aactttaatc tcacaaaaac taaaaagaca agacatcata
     3721 ccgtattaga aaagatggaa aaaaaccatt ttttcttttg gtatcatcat gccttgtctt
     3781 tttattggtt attcgttttt caacccttca tcacacaagt tttacatgtc attatcgtcc
     3841 ggcaacgttt ctgatgtttc ttcctgctca atgaatactt tccgaggctt gcttccttct
     3901 gacgggccaa caactccatg agcttccagt tcatctacta aacgtgctgc tcggttgtaa
     3961 ccgatccgaa atcttctttg gagaagggaa atacttgccg tttgcatttc aacgacaagt
     4021 gcttttgctt cttcaaacag ctcatcttgt ggctgttctg gagcacctgt cgtttcaact
     4081 tcatctgtcg gcatcatttt ctcttcataa tgagcttctt gctgatctgt aacaaattga
     4141 acgacccgtt ctacttcgtg gtcagaaatg aatgctcctt gcacacggat cggtttattc
     4201 tcacccattg gcaagaatag catatctcct cgccctaaga gcttttctgc cccattagaa
     4261 tcaatgatcg tccgagaatc tgtaccgctt gataccgcaa atgccattct ggaaggtaca
     4321 tttgctttga tgattcctgt gataacatcc acacttggac gttgagtagc taggatcata
     4381 tgaatccccg ctgcccgagc catttgcgct aaacgaataa tcgcatcttc aacttcattg
     4441 ctggctacca tcatcaaatc agctaattca tctacgatca ctacgatgaa tggtaaggtt
     4501 ggatgtttcg ttccgttttc caagtttttc tgttgaacaa attcattgta accagaaata
     4561 tttcttactc cggttgccgc gaatttttca tatcgttctt ccatttcttg tactactttt
     4621 tgtaaggctt gcgctgcttt tctagggttc gtcacgaccg gcgtcaacag atgaggtatg
     4681 ccgttgtaca tattcaattc aaccattttc ggatcgatca tcatcagttt tacttcatgg
     4741 ggcttagcac gcattaatat acttgtgata atcccattaa ttgctactga cttcccacta
     4801 ccagtcgaac cagccacgag taagtgaggc atcttcgaaa gatcagccgt ttgtacccgt
     4861 ccagaaacat cacgtcctaa aggtacttct agtaatttat ctgggtggtt tggttgtgct
     4921 tcgatgattt ctctaaagga aaccatactg atctttccat ttggtacttc aataccaatc
     4981 agtgatttac ctggaatcgg tgcttccatc cggacatctt tcgccgctaa agcaagcgcg
     5041 atatcatctg ttaagttcac aattttactt acttttaccc ctacagccgg ctggacttcg
     5101 aattttgtga cggatggtcc taagctggct ttgacgacct tggcatcgac accgaaactt
     5161 tggaaagttt gttctaatac gccgatattt ttttcaattt ttttatactc atcactttga
     5221 tccacagttg gtatagaatc aagcaaatca actgtcggta attcatagtc ttgattttcc
     5281 tgttctgctt caatctcaaa ttctaatgag gtaccgtcat cctctggcaa ctcatctgaa
     5341 aaaccttctg ctgtattggt ctgttttggt gtatctggta ctggtttctc tagattcgca
     5401 ggatggatat tttcttgaaa actgtcgatt ggaacaaaac ttagctgctc cggctctgta
     5461 ttttctggtt cctgccattc atcagcagta ttttcccttt gctggctcac cgcttttttc
     5521 ttctcatatt ctgcagcttc tttttctgca gcttcttttg cagctaagcg ccttgcttct
     5581 ttcgcttctg cgcgctgttt cattctctct tcctttgccg cttgctttcg agcttgcttg
     5641 gcaggatcac cttccaataa ttcctgtact cgttctccaa ttgattggat accatttaac
     5701 agctgatagc cttccagatt actgaacagg aatgctcctc ctgccagtaa tagcactgct
     5761 gctaaataac ttccaggctg tgcaatcaag aagtaagtaa aatgataaag gattgctcca
     5821 atcatcccac caccaacgtt attgtctact tgactttgtt ttatatcact ggctaacaaa
     5881 tcccatgtcg tccccatgat atttggttcc ccagtatgga gtttgccaaa taaatgggca
     5941 tgtaacaata ataagatacc gaaataaaag agtatgccgc caaaccagcg tcggttttta
     6001 ccaattgaaa actctgtatt cttaatgaca atccaaaatc ctaatacggc taataaaaga
     6061 cagaggattt ggtaagtatt tcctgcaatg atccgaaagc cgtttgcaat caatattcct
     6121 aaaaaaccta attttaaaaa gccgaagaca gcaaataaaa tcaaggcaaa cccaacacca
     6181 atcgtcagca tttgttcctg ctgctgtttt tgctttttcg tttttttcgt ctttttcctt
     6241 tttttagtag gccgtttctt ttgtgccatt tattatccct catttcatca agtttcatta
     6301 tacaaaaaaa agtaagggaa ctaaagtttt gtttgaaaga actgacgctt tctttcattt
     6361 ttcagcttat tttacagttt ttcttaattt ctaaatgatt ttatcaagaa aaggcacggc
     6421 catatccagc cgtaccttct tgttttaagt atgtgaacta aaggttctct ccattttctt
     6481 caatcagttt ttggtaccag taataactgt ctttcttata tcgttccaaa gatccttctt
     6541 gtgtttcgtc ttgatcaaca tatacaaatc catatcgctt ttgataaccg ttcagccagc
     6601 taagcaaatc tgtataagac caggtgcaat agcctaggac ttctgctcca tccgaaattg
     6661 cttctttgat ggctttgacg tggctttgta agtaattgat tcgatagtcg tcatggattt
     6721 gtttatcttc tgtcaacttg tcatactctc ctaagccatt ttccgtaatc agtaccggca
     6781 tgcggtaacg gcttgtgata cgacgcagtc cgatccttaa tccttctggg tcaatctccc
     6841 agtcccaatt tgttcgttcg acaaatggat tctcaacttt tttatacatt ccaggaactc
     6901 cagtttcttc tgatgaacct ttttttcctg tcgtattcat ttttccagca ccaactccgt
     6961 ttaatggatt gaatgcattc gtcgccgttt gatagtaatt gatcccaaga aaatctggtt
     7021 tagcagattc tagcaattct gcatcgccag cttcaatcgt tggtgcccat cctttttctt
     7081 ctaagaacct catggcagca atcggatatt ttccccacat atagacatcc atccaataat
     7141 tggccatcag gtcttctgca ttttcagcag ccagaacatt ttcaggatta ctgtcaattg
     7201 gataatttgg cgtatacgca aagcttggtc cgatttttcc aggcatttct aactcgtgaa
     7261 atttattgat gacagatgca tttgctaaat tagcaatatg gttaacttca tacattcgtt
     7321 tcgggtctgt cacacctggc ggatgtgctg ctaacaaata acctaatgaa gtgaagatat
     7381 tttgttcatt caaactaatc cagtaatgga ctttcccgcg gaatgcctca aataatgttt
     7441 ctgcataatg agtgaaatct tcaatgatct gacgtgattc ccaaccatta tattcatctt
     7501 gcaatgcttg tggaagatcc caatgatata aagtcaacat aggttcaata tcgttttcaa
     7561 tcaattcgtc gatcaatctc tcataaaaag cgagcccagc ttgattgact tctcctcgtc
     7621 cattaggaaa aatccgtgtc caagctacag agaagcggta agctttcaga ccttgttctt
     7681 tcattaaagc aatatcttct ttataacgat gatagtggtc tactgccaaa tcaccgttcg
     7741 ttcctttaaa tgtctttcca ggtatccgta caaatcgatc ccagactgat tctcctttcc
     7801 catcttcttg ccaagctcct tctacttgat acgctgcaga agcagagccc cacagaaaat
     7861 cttttggaaa tgaggtaagt tctttgtgtt ccattcatct cttcctttcc tataaccttc
     7921 aatcgttcct cttttagaat aacacgaaac cccttccaca acaaaataaa attatgtaaa
//
LOCUS       DACECR010000111         7976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.112, whole genome shotgun sequence.
ACCESSION   DACECR010000111 DACECR010000000
VERSION     DACECR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.112"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(3..1847)
                     /locus_tag="IWO17_002450"
     CDS             complement(3..1847)
                     /locus_tag="IWO17_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291306.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            2059..2577
                     /locus_tag="IWO17_002451"
     CDS             2059..2577
                     /locus_tag="IWO17_002451"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0291307.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            2705..3388
                     /locus_tag="IWO17_002452"
     CDS             2705..3388
                     /locus_tag="IWO17_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ0291308.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            3697..3770
                     /locus_tag="IWO17_002453"
     tRNA            3697..3770
                     /locus_tag="IWO17_002453"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3731..3733,aa:Arg,seq:tct)
     gene            3929..4288
                     /locus_tag="IWO17_002454"
     CDS             3929..4288
                     /locus_tag="IWO17_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ0291309.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            4411..6042
                     /locus_tag="IWO17_002455"
     CDS             4411..6042
                     /locus_tag="IWO17_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ0291310.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(6104..6745)
                     /locus_tag="IWO17_002456"
     CDS             complement(6104..6745)
                     /locus_tag="IWO17_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0291311.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            7027..7335
                     /gene="rplU"
                     /locus_tag="IWO17_002457"
     CDS             7027..7335
                     /gene="rplU"
                     /locus_tag="IWO17_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ0291312.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            7362..7706
                     /locus_tag="IWO17_002458"
     CDS             7362..7706
                     /locus_tag="IWO17_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ0291313.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            7719..>7976
                     /gene="rpmA"
                     /locus_tag="IWO17_002459"
     CDS             7719..>7976
                     /gene="rpmA"
                     /locus_tag="IWO17_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ0291314.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKK"
BASE COUNT         2529 a         1320 c         1638 g         2489 t
ORIGIN      
        1 ttttatacag gattcgggta atttcgagag agttgaacag tgatccgatc aatgaaagta
       61 tctttttcct gcgttagtgt tcctgtgtaa cgatccgcta cgtctttcat catcattaag
      121 tcaaaacgtt cctgatcatc taattcttct gtgatgcctc gactaaatag gtttgtatcg
      181 taatgtgcac tatgttcgca acaaatgacg aaatgttcct cttgttcgtg tatcgacaaa
      241 taaatttttc gatacagtgg attttcgatg gcatgagttt ctctaaagga atgctctaaa
      301 gcgtggatca aaagttgagc cagatcgtca tcgttgatag gaagttcttc gggaagatct
      361 gcttggaagg ttatctgaat attccgtttg gcagctaact tctggtaacg cgctaagata
      421 agatttgtca cctggtggtt gcaaaaagaa cacagcattt cgaaattttt tgcttcttct
      481 aatatctttt ccacatagtt tttagctttc gtttggtccg tatgcattaa atcttgcaga
      541 ttctccatct cttggacaaa ttccaggcga agtgtattta cttcttggat atgctgtcga
      601 agctgctcat aatgttgctc caacaaatca tttttgattt ttaagaaatc tacttgtctc
      661 cgatctttgt cggtatttaa gatgtgatcg attagattgt agctgataat aacgattaga
      721 atgataatat ataaaatcgt agaccaattg atcgttgttt gatagatacc taatgccgtt
      781 tggatgtaaa tcaagcaatg gataatggct accaagacga tccaaggtgt acaagctaca
      841 taaaaacggt tattcttata tgcttcgcca atgcatgcaa gtgaagtgac taaagtgctt
      901 ataacactag caatattagc aatcggcatt aattcaggaa gttctgcagg agtaaaagtt
      961 gcataaagca gaacgccgat aaaaatcaaa agttggaacg tacaaaaaat tccgtaccat
     1021 tttttatagt aaaacattcg gctccaatag tagcagatga tgaaagctga cgttaagata
     1081 ataaataaat gagataaaaa agaatgaacg aatggttcaa aaagaatacc acttaaaata
     1141 tcttctgata cagcttcaaa tcctaatgcc aaagcaaagc aacttaagag aataagtgag
     1201 tattcaagac gtttttgttt atacatcaag tagatcgtat agaaaaaggt ccctatagcg
     1261 ataaaaacag cgattatcat cgtcaatact tgcggcactg attgtgaata aataaaacta
     1321 atcaatggtc cagttgttcc tagataaact ttcggtggca gtccagcata gttctgataa
     1381 ggagatgaga ctgtaagggt caattttttt ccgatataat ttgctggtaa atctatcaca
     1441 gccaatgaga gtccgggatt gctttcttcc tgataatctt tccgttggta taaaatcttt
     1501 tcgcctagat atacagtaat ccattgatgg tttcctttga ttagtaaatt tgcttcgtcc
     1561 aacggttttt caagtgtcgt ttccataatg aatgtttcgt ctttatccac ggtaggaatc
     1621 gtacgagtgt acttgctgtc aaacttaaaa tcaggatcat cttttgtata gaaaacccac
     1681 gaatctttta tttctttcat ttgattgctg tccgcaggag aaaaaataaa ataaaagaaa
     1741 taagcaattc ctgttaaaaa taacaatgat aatataatga ttatgctcca ctttatacgt
     1801 aaataaaaag attctttttt attatatatg ttattaaaaa aagacataaa ggtattcctt
     1861 tctaaaactt gtaatgttag ggtaaacgtt ttttaaacta acgtcaatta tttataataa
     1921 aaaatgagat aaagatgagg aaatagaatt agaaacatat aattattaaa gggaaacatt
     1981 tttacagcta taatttgaaa aaaatttatc gttattatat gattaagaag gattgaataa
     2041 agaaagaagt tgaaaacgat acctgattac aaaaaaataa cttttataag ttctataacg
     2101 ttgattatat ttttaattct tattcttctc gtcgtcttgg ttccccgaat ttttggtttg
     2161 actccttata ttattgagga caaagaaatg agtccatatt atgcacaagg aaatctgata
     2221 tatgtacgac cgaaaaatac aaatgagatt ttagtcggag atattattac atattatgaa
     2281 aattcaggaa aaaaaatctc gacgcgtcgc gtaatagccg ttgaaaacaa ccacgaagat
     2341 ttttatacaa aagctgatac aaagacttat attgagcctg gagtagtaaa aaagagaaat
     2401 attatcggta gtcccatctt tcagattcca tatattggac tagtcctttc aaaaacggca
     2461 tttgcatgga ttcaggggtt attctttacg attgcttttt gcctaacagg aatcacagca
     2521 tggaatacct ttgtagagtt gaaaaagaag cggagacgaa cacagaaatt tttgtaagaa
     2581 tgttaaaaac ataaatgtat tccagagatc caacgactct tctgaacatg aaacacgaga
     2641 aaacactcat gcagcttgta gatttagtca gttgttcgta taatgtgtga agaggaggag
     2701 tagtatgcgc aatgaaatga tgcatcgtcc tgtagtcaaa gaagaactat tggattttat
     2761 gcgtacccag caaaaaaaat taccgggtga gctaggcaaa ctggaagaag aagctcatgt
     2821 agcagaagta ccagttattc cccatgaaac agttgtattt ttgaaatttt tacttggaca
     2881 gcttcagcct gaacgaatct tggagatcgg agcagctatc ggtttttcat caagtgtcat
     2941 ggccacgaca atcggagaaa acgctcatgt tacgacaatt gatcgtttcg atgtcatgat
     3001 cgagaaagct aaaaaaaact atgaacgtct aggactgaca gataaagtga ctttgcttga
     3061 aggtcaagca gcagatatct tgccggagtt gtctggtccg tatgacttta tttttatgga
     3121 tagtgcaaaa tcaaaatata ttgaattttt acctgaatgc ttacgtttgt taagaaaagg
     3181 tggcgttttg atggtagatg acatcttcca aggaggaacg atcttgctcc cagatgaaga
     3241 gattccccgt ggcaaacgag ctatccatcg taaattaaat gaatttcttc gtgttgtgat
     3301 ggatcatccc gatttgacat cgacaatctt gccattaggt gatggtgtga tcttaatgac
     3361 aaaggaatca gaaacaatcg agttgtgata catgattatg gcagcctgta gataatcgat
     3421 tgaatcgagg gctcaattcg aacttgatgt aaaagtttct atatttgtaa gtcaattaaa
     3481 ggatacagga aatctaaata aacaggagta gcatgaagtc aatgtagctc ctgttttcaa
     3541 ttgataacct tagaacagct gaaggagaat gtctcgtttt aaaagattga tttagtagtt
     3601 gggccaaaat aattcattat ttgctaatta aataactatt tcgtattgac agaagcaatt
     3661 taattatcta taattattga tgttccgctg attcttgtca tagtagctca gctggataga
     3721 gcatccgcct tctaagcgga cggtcggggg ttcgaatccc tcctgtgacg ctaaaaaacc
     3781 gttagaaact gaggtttcta acggtttttc tttgtttttt gtgccaatat tgtttgtttt
     3841 caaaggcaaa taaaataaaa aaacatcctt atcgagtaca attataagga gcaaaaaaat
     3901 aaaactcatc agaaaagagg aagaagatat gacgatacta tctagaattt tagtgactct
     3961 agttgcaatt gaattttttt atatcatgta tatcgaaacg gtgagaactg attctgacac
     4021 gacaagtagg gtttttaaaa tgagtaaaga agagttaagc agaaaatctg ttcaaacatt
     4081 gtttaagaac caaggagtgt acaatggact attaggagtg ggactacttt atggagctta
     4141 tctttcttcc gcatctaaag aaatcacaag tatgctatta atatctattt tttttgttgc
     4201 attatatggc agtttgacta gcgatcgttg gattttactc aaacaaggtg ggctagctct
     4261 gttggcattg ctttctattt tcttataaat atagaaaaaa taagaattta tctaacagga
     4321 aactttgtga aaaatattac tttatctcat gaaatatgat attctaatga atggtatttt
     4381 agaaaatttc atgaaaaagg agttagatag gtgttcaaaa attatgtttc ctcactaaaa
     4441 aaagaatttt caggttatga tctttcaaga tttcagaaag atcttttggc tgggatcaca
     4501 gtagctgctg tagcattgcc tttagccttg gcttttgggg tatcaagcgg agctgatgcg
     4561 gccgcaggtt tgattacggc tatcattgcc ggtatcgtta ttggcggttt atcaggcggt
     4621 ttttatcaaa tatcaggtcc gacaggtgcg atggcagcta tcttgatgtc tattgcggca
     4681 acaaagggaa tgcaaggtgt attgcttgct acttttttag cgggtctatt tttacttgca
     4741 gcaggacttt tccgattagg aacactgact tcttttatcc cagcacccgt aattacgggg
     4801 tttacttcag gtattgctat cattattgca ttaggtcaga tcgataattt atttggtgtt
     4861 cactcggaag gtacaaacgt tattgaaaaa ctaagtagtt atcaaacgat aggatttccg
     4921 ataaacacag cctctttgat catgggaggg ctagtcattt tagggatgtt tatctatcca
     4981 aaaaaatggg cgaaacgaat gcctagttcg ctgctggcta tcattttggc aacagcagtt
     5041 atgctattgt tccatttacc agtagcaact gtgggtaaga ttccacaaac attgatcagt
     5101 agtaatcgtt tgaatatggg agattttagc ttttctgcct tgcaacaagt ggcagttcca
     5161 gctattagca ttgctttatt aggaatgatt gaaagcttgc tatgtggtgc ttcggctggc
     5221 cgaatggcaa ataagccctt agatagcaat caagaattaa tggcgcaagg aattggaaat
     5281 ctgttgcttc ctttctttgg cgggattcct gctactgcgg ccatcgcccg tacaagcgtg
     5341 gccatcaaat ctggtgcaca aactagatta gctggtatga tacatgcagt tgtcttactg
     5401 ctctccatgc ttgtttttgc gccagtgatg tcacagattc caatgccagc acttgcaggt
     5461 gttttgattg tgacggcttg gcgtatgaat gaatgggaaa cgatcaagga gctatttact
     5521 aataaatatt ggtcagcagt tctattgttt atccttacga tgatttgtac agttatcttt
     5581 gatttaagta ttgctatcgt gatcggggtg atcggcggct gtgtattttt tgttgtcaaa
     5641 agcgctgcta tcacgatttc attagaaacg atcgattggc aaagaatggg gctgccagaa
     5701 acggaaaagc tgaataactg gacagtagtg tatattagtg gtccactgtt ttttatgtct
     5761 tcagaaaaac tcaaaaataa attggaagaa ctaaatcatc acgatggtgt cattttctcc
     5821 atgcgaggcg taccaagtat tgatttgacg gcacgtgcag tttttgatga atttcaagaa
     5881 aaagcagagc aaagaggaca aacgattatc tatacttcct tgcagccaga agtagaaaaa
     5941 caactttctc acttgtggga acatacattc acgcatcaac atcaaacagt tgctcatgcg
     6001 ttgacagctt tacatgcgaa atatactgca attagccatt aaaacaatga aaaagaacag
     6061 acaagactgt aaactgaaga ttttatggtc ttgtctgttc tttttaatat tgctgtaaaa
     6121 gtgaagctag tacttgccag tcttgatgga taacaggtac aagggagcga ttgtagaaaa
     6181 tagctgctgg atgaaaaagt ggaagaattc gatagttttt ttctgaccat tcgtaggcat
     6241 ccttttgttc tgttaattgt aagacaggcc ctgtatagag ttgaccatga tttttggtaa
     6301 ccgtatattc tttgccaagt aaacgttgaa gaccaatgtt tccaagtgtc gcaattaatt
     6361 ttggctggac cttcatcagt tcgtaatcca atattggtgc atgcgcaaat acttcggaac
     6421 gagtaggtgt ccgatttgga taaacagttt ctgactgttg tgtccgttta ttgatccgat
     6481 gagtcacgcg atacgggcgg ctccttacag cactggtgat atacacctct tctcgtgtta
     6541 agccagttga agaaagtgcc tgcatcaatt cttttccagc ttgaccgcta aaaggaacat
     6601 gcgtatcaat ctctgttttc cctggggctt ctcccacgag catcagttta ggatgaagag
     6661 gtccttgccc agcgataaat ccttctacag gacggccgtt cattcttaaa gaagcttctt
     6721 gtatcagttc ttttgggtat tccatttatg ctcctccttt ttcttttatt ataagaataa
     6781 aagaaacaca atgcgactat tttatttggt agaactgtga ggatagaaaa gcagaagtat
     6841 tgcttgaatt tttgttttac tttttgattt taaatttagt tgactaacgg ttgctgttct
     6901 ggtaagctag tactgttgta attgtggact ccacagctac aaccgctcag aacaagtaat
     6961 aagcacagca tatgctgtca cttcggatgg cgagtctaag tatatgagaa ggaggtgctt
     7021 tatagcatgt acgcaattat taaaactggt ggtaaacaag taaaagttga agtgggacaa
     7081 gcaatctatg ttgaaaaact tgacgttgaa gcaggagaaa aagttgtttt tgacgaagtt
     7141 atcttagtag gcggagaatc tacaaaagta ggcgctccaa ctgttgcagg tgcaactgtt
     7201 gaaggtactg ttgaaaaaca cggcaaacaa aagaaagtcg ttactttcaa atacaaacct
     7261 aaaaaacatt cacaccgtaa acaaggtcat cgtcaaccat atacaaaagt ggttatcgac
     7321 gcgatcaacg cttaatagcg tttgagaaag aaggcctata tatgatcaaa ggaacattta
     7381 agcgaaatga cgcaggtcag atcgtttcat tcactttgac cgggcatgct gatgctggtc
     7441 cttatggaag tgatatcgtt tgtgcgggtg tttctgcgtt agcgatcagc actgtcaacg
     7501 ggattgcctc attagccggt tttgagccga ttgttgaaat gaatgaagaa gaaggcggtt
     7561 atctttatac cgaagtcact tcaggaatga cacaagaaca aaacaatatt gctgagattc
     7621 tattagaaaa tcttttatta ggattacaat cgatagaagc tgaaaactca gaatatatcc
     7681 aaattaaaac aattaatgat aaataggagg tgcagaccat gttgttatct atgaatctac
     7741 aattatttgc ccacaaaaaa ggtggcggtt ctacatctaa cggccgtgac tcagaatcaa
     7801 aacgtctagg tgctaaacgt gctgatggac aaacagttac aggtggatca attctttacc
     7861 gtcaacgcgg tacaaaaatc tatccaggtg taaacgtggg tatcggtgga gacgacactt
     7921 tatttgcaaa agttgatggc gtagtacgtt tcgaacgtaa aggccgtaac aaaaaa
//
LOCUS       DACECR010000112         7761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.113, whole genome shotgun sequence.
ACCESSION   DACECR010000112 DACECR010000000
VERSION     DACECR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.113"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            147..1094
                     /locus_tag="IWO17_002460"
     CDS             147..1094
                     /locus_tag="IWO17_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0291315.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            1106..2026
                     /locus_tag="IWO17_002461"
     CDS             1106..2026
                     /locus_tag="IWO17_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0291316.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            2067..3524
                     /locus_tag="IWO17_002462"
     CDS             2067..3524
                     /locus_tag="IWO17_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0291317.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3869..4483
                     /locus_tag="IWO17_002463"
     CDS             3869..4483
                     /locus_tag="IWO17_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0291318.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4476..6200
                     /locus_tag="IWO17_002464"
     CDS             4476..6200
                     /locus_tag="IWO17_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0291319.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            6197..7648
                     /locus_tag="IWO17_002465"
     CDS             6197..7648
                     /locus_tag="IWO17_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0291320.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2618 a         1291 c         1543 g         2309 t
ORIGIN      
        1 ccgttttttt ctctaatact caggccaaaa cgctgatttc acaacctctc ctatactttg
       61 tttcattttc cctatacttt gtttatttat tctcattatt gaaagcgcta taattacgtt
      121 gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat cattttttta
      181 tgaatctatg gcgttataaa gcacttgtcc tgatggctgt accgggtatg ctttggatga
      241 tttttttctt ttacatacct gtaatggcga atgtcgtagc attcaaaaat tttcatattt
      301 cagcagatgg gttcttggct agcttgaggg aaagtccttg ggtgggttgg gaaaatttca
      361 aatttttatt ttcttcggat caagcatttc tcatcactaa aaatacaatt ttatataatg
      421 tagcatttat ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt atgagcgagt
      481 tgagaaataa acgactcgtt aaagtttatc aaacgatgtc cttactacct tattttctat
      541 cttgggtcat tatcagttac tttgtttacg cttttttaag tcctgacaaa ggaatattca
      601 atcagtggat cactggacat ggaggagaag ctgtcaattg gtataatgaa ccgaaatatt
      661 ggccgtttat cttggtgttc attggtacat ggaaagggat tggttataac agcattattt
      721 actttgcatc tgttatggga atcgatccga cttattatga agctgctatg gttgatggag
      781 ctagcaaatg gcagcagatc aaacatgtga cgataccaca acttattcca ctaatgacga
      841 ttttaacgat tttagccgta ggaaacattt tccgagctga ttttggcctg ttttataaca
      901 ttcctaggaa ttccggtgca ttatatgatg tgactcaagt tttggatacg tatatctaca
      961 atggcctgac atccacagga gattttggta tgacggctgc tgcaggactt taccaatcag
     1021 ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt gatagcgatt
     1081 ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg
     1141 ctctttcaat aagacggcta attttttctc taatataatg attgcactct ttgctatttc
     1201 ctgtattttg ccatttatat ttgttatcgc catctctttt accgatgagt ccatattgtc
     1261 gactcacggg tacagttttt ggccagaaaa gttcagtata tttggttata cgtacttgtt
     1321 cgaccagatg caggataaaa ttttccaagc gcttttcgtc actgtacttg ttactgtttt
     1381 aggtacactg atcaatagca cggctacttc tttatatgct tatgcgattt cacggtcaaa
     1441 ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt tctcaccagg
     1501 gatggtggcc aattatcttg ttatgacaaa tcttcttcag ttaaaagata cgatttgggc
     1561 attgatcttg ccaatggctg tcagtccctt caatattatt gtgatgcgca cattctttaa
     1621 acgttcggtc tctgattcga ttattgaatc ggcacggatc gatggagcta gcgaactgcg
     1681 aatatttgtt cagatcgttt taccgttagc tgttccaggt attgcgacaa tcagtttatt
     1741 tgccgcattg ggttactgga atgactggtt caatgcttta ctatacatcc aagatgataa
     1801 gctagttcca ctacagtatc ttttgatgaa aatccaaagt aatattcaat atctgacaca
     1861 aaattcagga gcaggaagcc aattgacagg cggattagcc tcagttccag gagaatctgc
     1921 cagaatggcg atcgtagtca tctcgacgtt gcctattgcg atcagctatc catttttcca
     1981 gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa acaaaaatat
     2041 aaatcataaa attaggagga agaaaaatga agttatggaa gaaactagcc tttacaggtg
     2101 tttcagcgtt gttgttggga acattagcag catgcggagg ttctggttct aaagaccagg
     2161 cagaagctag caattcggac actttgcaga tgtatcaaat cggagataaa ccagataatt
     2221 tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa agagatcggt gtaaaagtca
     2281 atatcaatta cattggctgg ggcgattacg aaaagaaaat gaacgtcatc atttcttccg
     2341 gggaaaatta tgatatcgcc tttgcgaaca actatgtttc caatgcccaa aaaggagcct
     2401 ttgctgattt gactgaatta gcgcctaagt acgctgaaaa agcttataat gatttagatg
     2461 aagcatatat taaaggaaat ctggtcaatg gaaaattata tgctttccca gtcaatggaa
     2521 atgtctttgc tcaacaagta cttactttta acaaagcttt actagataaa tacgatcttt
     2581 caattgatgg aatccaatct tacgctgatg cagaaaaagt cttgaaggaa ttccatgaaa
     2641 aagaaccaaa cacggcagct tttgccattg gacaaggctt caaagtccaa gggaactttg
     2701 attatccttt agggaatacg ttaccatttg ctattgatct ggatggagat gattcgaaaa
     2761 tcatcaacca atacgataat gaaacgtata tcggactgtt acgtacaatg catagctggt
     2821 acaaacaagg actcattcca agtgacgctg caacgagcaa taaggattat ccactagaag
     2881 gaaatacttg gctaatgcgt ggagaaacac aaggaccgta tgattatggc gatacaattt
     2941 tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat tactgttcca ctaaaatcta
     3001 cagctcaagc acaaatggca aattttgtcg tatcaaatac ttcaaaaaat aaagaaaaat
     3061 cagtagaact tctagggctg cttaacagtg atccagaatt attgaatggg cttgtttggg
     3121 ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga tgataaaatc aaattgctag
     3181 atggttatca gccaaataca catatgtctg cttggaatac aggaaacaac aagattctat
     3241 atacacaaga gtctattacg gatgacatga ttgccaaacg tgatcaatcg attgctgatg
     3301 cagaaacttc accgatttta ggattcagct tcaacactga cagtgtcaaa acagaattaa
     3361 gtaatatttc taatgtcatg aatcaatatt tagatggatt gaatacaggg actgttgatc
     3421 cagatgaaac attaccgaaa ttgaaagacg ctttggacaa ggctggctat gataaggtat
     3481 tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga ataataatga ataaagacaa
     3541 aggggttgtg acaaaagacg tgtcacaacc tcttgttccg aataaacggt gttcaaaagc
     3601 cagtttctcg gtattaagct aaatatatgt aaaaatgtgg aaagccatat tcacaaaatt
     3661 tttcttacta ctcgaaacta agcggctatt tcacaaactc ttggtaaacg gtgttcaatc
     3721 agctaaccct cggtattcgc tcacaaaacg acaaaatatg aggaactatt tttccgtttg
     3781 tttctctaat aatcaggtca aggtgctgat tgcccaacct ttttttgtat aatgagaaag
     3841 tataaactta atgtttggag gagaagatat gttagggaag gttttggaag agttatttat
     3901 ccggatctgg gtcattataa aactaacctt gtatttctgg atatatacat ttgccggcgg
     3961 gatcatcttt ggattaggtg cggcatggaa aacagtcaat gaactatttt atttgtacgg
     4021 attcgagtat aaagaaatca caataaaaag aggctggaat atatataaaa gaaatttttt
     4081 aagaggcaat ctgctattta gtttattttt atctggtact gctttattga gttacaattt
     4141 atatttatct gttcaaatca aagggctcat ttttttagct attgatttta ttttactttt
     4201 tgccttgctc tgcctatttg ctacttatca atacagtatg attttagata gtgaatatac
     4261 gatctctgtt cctaatctgc taaagctaag ttttatttcg gttttttcca gcttttcgtc
     4321 ttttctaaaa ataattattg gtagcggtat aattttaggc gtgacttggc aattcaaagg
     4381 attgatcctg tttggtgtca ttggtctttt gacggtttgg aatggtacga tgacaacaca
     4441 ttggcgtgaa gaattagata aacagttgga aagttatgaa taagtttcgg attctatttt
     4501 caaaaggaac cttactaaat cgtctgttgc ggaaatacgc ggttatcatg atcggtgtaa
     4561 cgatgactgc taccgttatt tttagtatcc atacttggga acaaaatcaa aaacaggcag
     4621 aaaacatgac aagtgatgcc gttcaaagca caagcagaat gctgaatgat aaaacaacct
     4681 tgagtaggat catcaaaaac cagcttgtag ggaattccga aaagatcgaa aatgtgacga
     4741 cctacttaac aaaacccata gatcagtatc tgatgtatgt atacgaacag caaaacagta
     4801 ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct ctacgcaaat tatgaagaac
     4861 taagtgcgat atatatcgtg ttgaatcaac taccggagta ttatgaatct tctagagaaa
     4921 acaaaggtgg ggaaataaaa aaggggacac caaaattact agacgccttt tatatcaagt
     4981 tgcctatcac acaaggagga gctgagtcag gaagtatttt tattggtttt caaaagaaag
     5041 aactagatat gattttagaa aatctaacgt cattcaatgg actctcttta tacatgattt
     5101 ctggaacgac aaatcgacta tacacattcc atgataataa aatagctaaa gaaacgtttg
     5161 aaagacaaga aatgatcatc acagagagtc tgaagaaaaa ttcacaattg ccgattgacg
     5221 atctggaaaa aaacaatttt gttcaacacc aagaactgtc tgatgattat cgtatattag
     5281 ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa aaatcttgcc ccgctgatca
     5341 ttggtatcat tattttagac aatatactgc ttatctttct ttataaaaca ttcaaacgat
     5401 attcacagca agtatctatc gtcatgcagg ccatggatca aacagcagca gggaatttag
     5461 agacgcggat cgacataaca aacacggaat atgaactgaa ggaactttca atcggtatca
     5521 acgaaatgtt ggatagcatc aatcagtttg tagaagacat ctacaaatta gaaatcaaac
     5581 agcaagacgc acatatgcgg gcgcttcaag cacagatcaa tccacacttt ttatataata
     5641 cattagaata tatccgaatg tatgcaatca gcgaaggaag tgaagaatta gcagatgttg
     5701 tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca agagaaaacg atcactttaa
     5761 aagaagaact ggacttttgc gaaaaatatg tctatttgta tcaaatgcgt tatcctaatc
     5821 gagtagccta tcattttatg atcgatcctg atttggaaaa gatcgaagta cctaaatttg
     5881 tgatccagcc attagttgaa aattatttta aacacggtat tgattttact cgattcgata
     5941 atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt aaggatcatc atcaaagata
     6001 atggaaaagg gatgactgaa aaacggctaa aacagattga agaaaaactg tctcatccta
     6061 aagtggaact tcacggttct atcggtctgc aaaacgtgaa tgaacgttta cgtgccagct
     6121 tcggcagcag ttattatatg tcgcttgaga ataatgagac aggtggactg accgtttcta
     6181 ttacattcaa ggagggatga gcgtgtatca agtgttattg gtagatgatg aatacatgat
     6241 tttgaatggg ttaaaaaaga taattgattg gcaaagttta ggctttcaaa tagtagcaac
     6301 agcagagaat gccaaagaag gactagctgt acttgaacag cgacagattg atttagtagt
     6361 aacggatgtg acaatgccag aaataaatgg actggaattt attgaagcgg ctcaaaaaga
     6421 acgtcataat tttgaattta tgattttatc aggataccaa gaattcgatt atttgaaagg
     6481 aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc gtgaataagt tcgaattggt
     6541 tgaaagcctc aagaaaatca aaacacggtt agatcagcaa aatgaacaaa aaaatcaaca
     6601 agaaatctat caagaactgt tattcagtca atggttgaat gaagaattgg atgaaataag
     6661 tgaagaagaa ataataggac aaataagtga gaagcagcat aggatagtat tgattcaact
     6721 ggcaagaact catgatactc ttatcaatag atggttgcaa gaacggcaac aaatctttta
     6781 ctatcaacga aattacggag accgtgtatt gatcacgttg ttgttggagg cagatgaagt
     6841 tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat caggaatggc tgatcagtat
     6901 cggggaagag acagattctt tggacaaaat ccctgaaaca ttccaacaag cgaaagacaa
     6961 tctacagttg catcaatttt atggagataa agacgagcat gttttttacg cagtacaagc
     7021 agaaacaaaa gatcaaacca tcgatttttc aagtttcaga cgcgtgctgc aaaataaaca
     7081 attggaagtc gctcaaaaaa tgatcactga tttttttgaa cagtttcagt tagcagccat
     7141 gatgccggaa gatatacgat attccagttt tttactgttt atggagattc aacgggaatt
     7201 gattgatctg gaagatgagg aatatttaca aggaatcgaa aaaatccatc aggcaaaaac
     7261 ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa aagcatcaga gacagaaaaa
     7321 gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac cagcattatc aagaaccact
     7381 tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc atgtatctag gacagctgtt
     7441 caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac catttacgta tggaaaaagc
     7501 gaaacaactg cttttgcaca gtaatcaaaa catcaatgag atagcaagtg agattggcta
     7561 caacaacacc acgtatttct ctaaattatt caagaaaatc gtcggcagat cccctaaaga
     7621 gtaccgagaa aacagtggag aatcataaaa gaaaaagcca atgtaatgaa ataacttata
     7681 aaaaattgtc tagaaatcta attttgggct gttcttatta cttgaggcaa ggcagctttt
     7741 tcatgacctc tcgataaacg g
//
LOCUS       DACECR010000113         7630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.114, whole genome shotgun sequence.
ACCESSION   DACECR010000113 DACECR010000000
VERSION     DACECR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.114"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(85..252)
                     /locus_tag="IWO17_002466"
     CDS             complement(85..252)
                     /locus_tag="IWO17_002466"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291321.1"
                     /translation="MKDIQSGNVQETIDKWKGKHYRFTEAQKQALEYYQKLEESLRFN
                     DEARKYLKELL"
     gene            complement(268..1362)
                     /locus_tag="IWO17_002467"
     CDS             complement(268..1362)
                     /locus_tag="IWO17_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291322.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSK"
     gene            complement(1441..2085)
                     /gene="pcp"
                     /locus_tag="IWO17_002468"
     CDS             complement(1441..2085)
                     /gene="pcp"
                     /locus_tag="IWO17_002468"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0291323.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(2284..3228)
                     /locus_tag="IWO17_002469"
     CDS             complement(2284..3228)
                     /locus_tag="IWO17_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0291324.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(3230..3916)
                     /locus_tag="IWO17_002470"
     CDS             complement(3230..3916)
                     /locus_tag="IWO17_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0291325.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            4389..4709
                     /locus_tag="IWO17_002471"
     CDS             4389..4709
                     /locus_tag="IWO17_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0291326.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            4870..5775
                     /locus_tag="IWO17_002472"
     CDS             4870..5775
                     /locus_tag="IWO17_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0291327.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            5772..6545
                     /locus_tag="IWO17_002473"
     CDS             5772..6545
                     /locus_tag="IWO17_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0291328.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(6722..>7630)
                     /locus_tag="IWO17_002474"
     CDS             complement(6722..>7630)
                     /locus_tag="IWO17_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0291329.1"
                     /translation="AISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVF
                     VGIEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPA
                     MGYVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNK
                     NGAPTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQR
                     KEWVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHL
                     DKILLIGVTMVGVIAMVLFATGMLHV"
BASE COUNT         2291 a         1570 c         1356 g         2413 t
ORIGIN      
        1 aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa
       61 cgcttctgtc ccagcctcta ttttttatag taattccttc agatactttc ttgcctcatc
      121 attgaaacgc agtgattctt ctaacttctg ataatattcc aacgcttgtt tctgagcttc
      181 agtaaaacga taatgttttc ctttccattt gtcgattgtc tcttgcacat ttccagattg
      241 gatatctttc actactagtt gccattttta ttttgagact gtacggtctt tcttatcagc
      301 agattgtata gcttctcgtt ctttacgcaa tacttttaaa aaaccatttc tgactatccg
      361 aggaatgctg tcgaagaacc cttctgtaaa accatagcga caatattgct tccaaataaa
      421 tagatagatt tttggttgga tgaatcgttg gtttttcgca aaatattcca tctgagcagt
      481 gacttctggt gttcgttgac gttttgtctt tctcaacacg gaaataaatg tttcaaaata
      541 agcattagga tgtactggca cttcttcata tacgatggat tgccaatctt tacgtaaaaa
      601 tgcgcgatag ttgatcaaga attcttctac atcctttact aattcttctg caaagtcgtc
      661 aaatagttct ttttttatct gtcgcttttt tgactgccca ttgatgtgtt cacttatttt
      721 cttgatttca ttttccaatg cttgataatc cgctatgtaa atcggcattt ctacttggcg
      781 aaattctgcg agccgctgag ctaattttat atccggtaaa tatggctgtt tcatccgacg
      841 attttggtac aagacaaaac agtatttttg ccatagtcct tcaggaaaat cgatattctt
      901 ctgcttaagc cataataatt cgtctaaggc cagatcaact tttgtcattc cctgatggat
      961 cgaagctgcc actgtcggag agacatgcag gtgatcggca aagtggttga tgcctagttt
     1021 taggatcttt ccggtttttg gcgatttaac gatgtattgc acttttaatt ctctaccaca
     1081 ctcacaatac aatttttgct ggcttcgttt tgttcgaaaa aacggattga tcttgaagtc
     1141 agaaaacacc caatctgttg cagctaaata atgttcttcc ataaaacgag agctgattag
     1201 atatcggcgg tgcttatcga ctagttcccg ctgttttttc gttaattttg catagatctc
     1261 ccaacgttga ctgcttgtgt attgtttaga aattttgatg ggatagcttt tgctgatttc
     1321 ttttggaaac cattcttcaa agacggattg ccattcttcc acggctgttt ctttcctttc
     1381 taattacaaa aagctggcta ctaacataac gttagcaacc agccagtgtt tcttgctaaa
     1441 ttagtgagtt gttccggcat ttccggaaat atcttcttta ttttctactg ctgcttcaac
     1501 tgcataagtc aaagaagaag caatgtcttc taaagacata gaaggcgtac ccactggacg
     1561 atcgatcact tgatcgggtg agaatggcac gtggatgaac ccacctcgaa ttgccgggaa
     1621 ttgtgtttcg atcaaatgaa gcaaacgata catgatatca ttgcagacaa atgttcctgc
     1681 tgtataagag atgtacgctg gtagaccatg atcttgtaca ttttttgtca ttgctttgat
     1741 tggcaatgag gtaaagtaag cggctggacc gttttcttct aaagccgtat cgaatggctg
     1801 atttccttca ttatcaggga ttcttgcttc tgctagattg attgcgacac gttcgaacga
     1861 gactgctgac cgcccacctg cttgtccgac acagatcacg acatctggtt ggtgctgttg
     1921 gatggcttct ttgactactt gactgctttt ttcgaatacg gtcggtactt ccactttgat
     1981 gatttcagca ccgctgattt catctggcat tttttttact gcttcatagg ctggattcac
     2041 tttgtcccca ccaaatggat caaaacctgt tactagtact ttcatctgtt gttcctccgt
     2101 ccgattgatt taacatatgg ttatctttta tcataccatt ttccttgaac agcgtaaata
     2161 aaggaaacaa tagtttaaat aacataacag atcaaactta atatattctt ataaaaaaat
     2221 agtgttccac tacaatgtta gaagaggttg cgacaattat tctgtcacaa cccctctctt
     2281 ctattattta aatagaatca tataagcaat ttggaaaact agcataaata acgcaataaa
     2341 caactggttt ttgatcactc cgtatttgtc tttcatctcc agcatcgcta caggcacgat
     2401 attgaagtta gcagccatcg gtgtcagcag ggtcccacaa aaaccgcaag tcaatgcaac
     2461 cattcctatt aacgctggat tcgctccatg actaaatacg aatggtgcac cgatcccaac
     2521 cgtcatcacg gtgatggccg caaatgcatt ccccatgatt accgtaaata tcaccatgcc
     2581 aagagcgtaa atgatgatgc cgatgttcac atttccttct ggaacgatcg tacctactgc
     2641 attggaaatc actgtcccaa ctccagcgct tgtaaatacc gcacctagag aagctaaaag
     2701 cattggcaac atgcttaacg gccctactgt gcccagcata tctgctgcat catctaaaaa
     2761 tgtacttggt cgattatctc gagaaaagaa catcaagatt agcatggctg caaaaacacc
     2821 gacgcccacg ccgactaaag cacctaaatt ggtaaataga gcaaacaaga tcgcaaagac
     2881 accgatactc aaagctggaa taaaaatttt cattcctagt ttgtctgaca ttttttccat
     2941 atattctttt gatggaagcc ggctttctcc tttgctgact ttcttagcaa ttgctggtat
     3001 ggtcatcgca aagatcaagc caccgttcac gattgccgga agccatcttc ctcctgcaat
     3061 aactacccct aagaccgtcc agaaaaaggc tgacccataa cgatgaggat tggttttatc
     3121 tgttaaattt cttactccag tgtaaatcaa tacgagtccc atcacgatat aaaggatctc
     3181 cagtaattta tcgcctaata aaatatcagg atttgtaaaa aagtccatct tattgcccct
     3241 ttcctggata atatttcttc gctaatttct tttctttaag atagaagtag acacttgcca
     3301 caactaaagc cgtgatagcc actggaactt ccactaaagc aagctgtccc aatgttacgt
     3361 catatcctag atctttcagt gttgattgga ctagcaacgc ccctgctcct ccgatgaata
     3421 gtacttggcc aaagaaccag cagacatttt ccatagctga agccatacct tttagttctt
     3481 cttcatgctt aggatggact tctaaccctt ttgattcgat ggtccccaat gccatcggta
     3541 aaatgattgg acgaacaaat ccagcaactc caccaaaact cacgttaaaa gccgcaaaga
     3601 tcattcgaaa gatcccataa atatctacaa tcataccagc agatactttc ttgaatcgct
     3661 tgatcaagga agccgctgat tctttcagac catttctttc aagcgttcct gttgccaaca
     3721 taatgatgat aaaaatcgcc atcccgcgat tgtttacaaa acttgtacct aaaagttcta
     3781 acattccttg aatgccaaga ccgccagtca aagctgtaac aactaaagca accattacga
     3841 tcaatatcga gtctaattta aatgcaaacc cgagaataac aatcaagacg cctaaaaggc
     3901 taagataatt ttccatatat tttcctccat aaaatgacaa tattttttta attataaagc
     3961 aaaacacata aaaaatatag ttgttttctc acgaaataga cactattttt aaatattttt
     4021 cgttattttt ttattatata attcacctta cttattgtaa gataaagctt tttttaattt
     4081 attgcctgct aatatataaa aataaggtct agttactgtt tttttaaatg attaataaat
     4141 aacaactcct ttctatgaaa ttgcaaagca ttcatcttct tttctgctaa cgatatatcc
     4201 gtcaatgctt gttacacttt actaaaaagt atgagattgc cgatatctgg ataggtgtgc
     4261 tatactacag aaaaagatat acgatctgcc accgggtagt aaaaaagagg atgatcattc
     4321 cattaggtct ttgaagggat gattctcctc tttttgtgta ttttttatat tgaaggagga
     4381 acgtatgtat ggcatggatg gaattaatca tagcaggaat tttggaagta ttctggtcaa
     4441 cgatgatgaa atggagtgat ggtttttcta aaatcaatta ttctttttac acagtgattg
     4501 gaatgattgc tagtttttac tttttgtcaa aggcaatcaa aagtttgcct atgagtctct
     4561 cttatcctat ttggacaggg attggcgcag ttggttcggt cattattggt gtggtcttgt
     4621 ttcatgataa gctgaatctt tccacttggt tctttgtcgg gttgctgctc atcagcatca
     4681 ttggcatcaa aatcactagc ggtcactaga tacttagaca agcagacaag gactgatcag
     4741 aaaaagcggg gctttttatt tgatattcct ctttctatga aaaaacaaga aaataagact
     4801 aatgacctag cgacttctct actcatcagg tgtatgcttg tactatctat agaaaaggag
     4861 tgaatatttt tgaaaagtct ttcggtccaa gaattatcca agaactttgg tcacaaacaa
     4921 gccctcagcg atgtcagttt tagcttatct cctggcaaaa tcgttggatt agtaggtcct
     4981 aatggcgctg gaaaaacaac gatcatgaaa gctattttag gactgatcca ttactcagaa
     5041 ggaacgatcc gtattggtga aaatgagatt tctccctcta gtcataaagg attagaacaa
     5101 gtaggagcat tgatcgaata tccgggtatc tatccattct taacaggtta tgatcatctg
     5161 aagttattct ctgaaacaaa cgatgtatct gctatcgaca ctatcgtgaa acagctcaag
     5221 atggaaaaat acattcacaa aaaagcgaaa tcctactcgc tgggaatgaa acaaaaattg
     5281 ggaattgctc ttgccttact gaaccatccg tctatcgtta ttttagatga gccgatgaac
     5341 ggtttggatc cacaagcaac aagagatgtg cgaaaaatca ttaccgactt ggctactcag
     5401 ggcacctctt tcttgatctc cagtcactta ctcagcgagc ttgaaaaaat ggtcgatgag
     5461 ctgatcctga tcaatcaagg aaaaatcatc aaacaatgta caatggctga attgaaccaa
     5521 acagcgcaag atttcatcgt tatctctacg acaaatgatc aagctgctcg aacgatcttg
     5581 acagacgctg gatatccctt ggaagatcct gataaaatca agcttctaaa aaaaaacgaa
     5641 gagttattag gaactgttat ccagttattg tctgcagaaa atgtccatgt gacagatgtc
     5701 cagcatctga cgaatgattt ggaatcttcc ctattggaaa ttctggattc agtaaagggg
     5761 gaagaagaaa aatgacgttg cttataaaac aagaattatt taaattgatc aagaaaaagt
     5821 caactgctgt tttatctgtt ctattagttg ctttgctcat aggaacagca cttctagcca
     5881 aaaaatacac gacgatcatt gatccagttg aaatgactgc tcagcttttc agtgccacat
     5941 cttggattgt ctttatcatg attgctgctg caagtacgat catttcaatg gaatcacaat
     6001 atggtacatt gaaaaatctc ctttatcgaa agtattctcg aggagaaata ttagtaagta
     6061 aatggatcac attagttatt tattctattt atttatacct tttagctatt attgtcaccg
     6121 tgctgatgaa actgatcctt ttcccttcga tttcctttac ggagaagatt tctactggtc
     6181 agacactgat ccaaagctta ttcacttata ctttgggtag ttatatcggt ttatggctaa
     6241 ttttaagttt agtgttgatg attgcttgtt ttatcaatag ttccggtgcc tccatctcag
     6301 cagggatcgt cttttatttt gcttcatcaa tcatatctgg tatcctcatc gcactcattc
     6361 aaaaatggga gtggatcaaa tggaatccaa tcagtatgct gaatctgcaa aaccaaattg
     6421 gcaacgaaga gattatgaag caattgactc atctttctac taatcaaatg ctgtttggca
     6481 atttagctta cattgctttg ttcttagcat tgggatacct cgtattcaag cgaaaaaatg
     6541 tatgatggtt cattaacaaa gaagttgtga cagaagtggc cagtttcaag aaatttcggc
     6601 ttatttttag aagaagctac ttcttgaaca ccatttattc aaaaaaagaa gcgatgacac
     6661 gaatgttgtc accgcttctt ttttgaataa accacaggct tcacagcctc tctttatact
     6721 tctatacgtg aagcatccca gtggcaaata aaaccattgc gattactccg accatcgtca
     6781 caccgataag taaaatctta tctaagtgga tatgttgtac aggttcgttt tgttctttac
     6841 gtgctttgag gtaaaaataa atacctggta agaaaagaac aagaacaagc atcacttcat
     6901 taaaggctac aactagcatg gcccatactt gaaaagcact tgctaaaaac cctactaaaa
     6961 attggaccca ctctttccgc tgataagaaa gcttcatttg gtacagccct aagaaaagcc
     7021 acgtgaagaa aatcgctgct gtaccaagtg tataagcaaa gttatatgca ctgtcagtga
     7081 ataataatga aagtaaaaag acattcgtac ataacgtcgt caatacgata gcataagttg
     7141 gtgccccatt tttgttgagt tttccccatg ctttaggcaa aagtccgtcc tcagccatat
     7201 tccccattgt ttcaatcggc aagattgtcc atgagagcca aaccccaact aaagaaatga
     7261 tcaaaccgcc gttgatcacg accgcccccc atttaccaac catcatttca aatacatatc
     7321 ccatagcagg ttgcgctttg atcgtttgca gctgattcat cgtcattgtg ccatatggaa
     7381 gcatggaaac caagacgtaa ataatgatca aaccgaatat tcctagaatc gttgctttcc
     7441 caacatccga ttgttttttt gctcgtgaac ctacgacaga agctccttca ataccaacga
     7501 atacccagag catcaccatg atgcaactca atacttgact agaaaacggg atcggctgcc
     7561 cattcgcatt caatatgata tcgctagaaa agttattaat gaattgttgg atcgaaaagg
     7621 tgctgatagc
//
LOCUS       DACECR010000114         7630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.115, whole genome shotgun sequence.
ACCESSION   DACECR010000114 DACECR010000000
VERSION     DACECR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.115"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(113..637)
                     /locus_tag="IWO17_002475"
     CDS             complement(113..637)
                     /locus_tag="IWO17_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0291330.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(630..1103)
                     /gene="tsaE"
                     /locus_tag="IWO17_002476"
     CDS             complement(630..1103)
                     /gene="tsaE"
                     /locus_tag="IWO17_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ0291331.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1226..2206)
                     /gene="pta"
                     /locus_tag="IWO17_002477"
     CDS             complement(1226..2206)
                     /gene="pta"
                     /locus_tag="IWO17_002477"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ0291332.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2359..3039)
                     /locus_tag="IWO17_002478"
     CDS             complement(2359..3039)
                     /locus_tag="IWO17_002478"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0291333.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3165..4034
                     /locus_tag="IWO17_002479"
     CDS             3165..4034
                     /locus_tag="IWO17_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0291334.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4060..4575
                     /locus_tag="IWO17_002480"
     CDS             4060..4575
                     /locus_tag="IWO17_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291335.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4622..5185)
                     /locus_tag="IWO17_002481"
     CDS             complement(4622..5185)
                     /locus_tag="IWO17_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0291336.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5240..5419)
                     /locus_tag="IWO17_002482"
     CDS             complement(5240..5419)
                     /locus_tag="IWO17_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291337.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5496..6005)
                     /locus_tag="IWO17_002483"
     CDS             complement(5496..6005)
                     /locus_tag="IWO17_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291338.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6101..6481)
                     /locus_tag="IWO17_002484"
     CDS             complement(6101..6481)
                     /locus_tag="IWO17_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0291339.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6811..7560
                     /locus_tag="IWO17_002485"
     CDS             6811..7560
                     /locus_tag="IWO17_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ0291340.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSRGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKLTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2171 a         1582 c         1356 g         2521 t
ORIGIN      
        1 acaccgttta ttcaaaaaaa atggactgcg acacgacttt tgtcacagtc ccttcgtgtg
       61 tttttctgcc ttctcaaaag gatgacagtt tctcactctt ttcctatttc ttctattcaa
      121 tcaaataact cattttatat acatctaaaa attcacctag atctgttctt gcccctctag
      181 gcatgaccgc ttcgatttgg aagcccattt ttcggtaaag gtgtacggct ctttcattac
      241 gaacttgaac gtcaagctcc aatcgaaaaa gtacacccat ttccttcgcc caactgatga
      301 tttcttctag cataagagtg cctagtccca ttccccaata ttcctgcaga atactgatac
      361 cgacttctcc cacgtgagac aaacgaaatt gactgtctgc cttgacagat gctgttccaa
      421 taatcgtccc ttcatagatt gcaagcagca ataaattatt attgctttcg tatatataat
      481 ccaactcttc ttctaaggtt tctggtggta acaggagttc tgcttcatcc aaaactaaaa
      541 attctgtctc cttattaact tgtcccatca tcaaaagaat gtcttttgca tctcctggca
      601 ctgcttcccg caagatgatc ccaacttctt cactcatgat tttcagccca tttttctaga
      661 attcgctgtt tgaaggtttc actttgctcc ccgtatgaga ttagtttcac ataccgcttt
      721 tctaaatctg tatcactttt ttctaaaatc agttccaaat aatcctccgg cagtgcttct
      781 tctaatagat ttccccattc aacaacggaa agcccatcac cttcaaaata ttcatctaac
      841 cctaaatctg ctccgctagc tgctacccga taaatatcca tatgatacaa cggaatccga
      901 ccttgactat attctctaat aatcgtgtat gtcggacttt tgatcatttg cgttatccct
      961 aatccttggg caattccttt cgtcaacgtt gttttacccg cacctagatc accagtcaat
     1021 atcagattgt cacctggcat tgcggaatga ccaattgcct ctgcgaattg ttctgtcatt
     1081 gttaatcctg atagttcgat catttttcat cttctcccgc tcttctttct ttttgccagt
     1141 atatcgcgta atgaagtttt tttaaagaaa aaatgaacta gtcaagaaca gcagctgctg
     1201 tttttggcta gttcgcgtgt atttgttaat caatcaatgt ttgtgctgct gtgataatcg
     1261 ataatttata tacatcttct tcatttgctc cacgagacaa atcagaaacc ggtttgttca
     1321 agccttgtag aattggtccg attgcttcaa agttaccgaa acgctgagca attttatagc
     1381 cgatatttcc tgactgcaat tctggaaata caaagactgt tgcttgacca gctacttctg
     1441 aatctggcgc tttcagttgt gcaactgaag gaacataaga agcatcaaat tgcaattcgc
     1501 cgtcgatcat gtattgtggc gctagttttt tagcgatttt agttgcttca actactttgt
     1561 ctacttctgg cgatttcgct gagccttttg tcgagaagct catcattgct acttttggtt
     1621 cgatatcgaa caattctgct gtttttgcag aatctacggc gatttcagcc aattcttgcg
     1681 cagttggatt cacgttgatc gcacaatccg aaaagacata tttttcttga tctcttccac
     1741 gtaccatgat catcgctccg cttgtccgac tgatgccagg ctttgttttg atgatctgaa
     1801 gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc agagaccatt ccgtctgcaa
     1861 tccccatata cgtaagcatc gtaccaaagt aattcacatc ttttaaaatc ttttctgctt
     1921 gttctttgga tgcctttcca tttctacgtt caacaaaagt atccaccatt tcatcccact
     1981 tatcgtaatt atcagggctg atgatcgtaa agctagatgt tttgattcct ctggcatgcg
     2041 ctgcatttct tatttcttct tcgttcccta ctaaaatggg ttccacaagt tcttctgctt
     2101 tcaaccgggc agctgcgcct aaaatacgag catcagtagc ttccgggaaa acgattttga
     2161 tcccacggcg aacaatttta aatcttaaac tatcaaacaa ttccacaaga tgacctccag
     2221 acgtttttat atgtattatc atacactatt ccagaaaaaa agaacagttt attttatctg
     2281 tatcagtcaa aaatgctgga aaaattaagt ttttcacata caaagcgaac tgcactataa
     2341 caggaaagcg ctgttttatt acactgtttc aggcagttgc caatcaatcg gttcttctcc
     2401 taacgcaagg agcgcatcat tcgttttcga gaagggtttg gaaccaaaaa agccacgact
     2461 tgcagataac ggactgggat gtggagatgt tatgatgata tggcgactct tatcgatcat
     2521 tttgattttt tcttgtgccg gttttcccca caagataaac acgattggtt tttcccgttc
     2581 atttaatttt tcaatgatcg tgtctgttaa tctttcccaa cctttcccgc gatgagaata
     2641 cgcttgccct tcccgaacgg taagaacggt gttgagtaaa aggactccct gtttagccca
     2701 tgacactaga tttccatgtt gaacaggtgc gattccaaga tcatcataaa gctctttata
     2761 gatatttctt aacgatggcg ggatctttac gccaggttgc acagaaaaag ataagccatg
     2821 agcttgattt tctccatgat aaggatcttg tcctaaaatc accactttaa cttcttcata
     2881 aggagtcaat tccaatgctt catagatatg atacatatct ggatggatct tttgggacgc
     2941 atattctttt tttaaaaatt ctcttagatg cagataataa tcttttgaaa attctgagga
     3001 caaaacttct tgccaactgt tatgaatgat ttctttcaaa aactccacct cctaataaaa
     3061 agaataactc acattttctt caatcacaag ggacaatctt caccctcagc acttgattaa
     3121 tatggtaaac taggaacaag aagcagaaag gaacgataat cattatgatc aaattaattg
     3181 catcagatat ggacggcaca ttattagatg cccatatgag tgtttctcct gaaaatgccg
     3241 aggcgatacg ttttgcaaat gatgccggtg ttgagttcat ggtcgctact ggtcgaaact
     3301 atcaagaagc tcgcgcagct ttagatgagg taggaatcga ttgtgcgatg atcactttga
     3361 acggggcaca ggtatttgat aaagaaggaa attcattgtt taccgtgtcg attccaaata
     3421 atcaagcaat ctctgtttta gatattctag atgctaacgg tatttactat gaagtagcaa
     3481 ccaacgatgg tctttattct gaaaatcaag cgaaacgaat tgaaagtttt gcttccatgg
     3541 tggctaccca tttacctcac cttacgtata aaatggcgat tgccatggcc tcagctaagc
     3601 tggagttatt gcacatcaca tacgtggaca gtattcgtgc tattttggaa gaaaagaatc
     3661 tggaagtttt aaaaattatt tgttttcata cagaagggcc agctgtatta ggtccagttg
     3721 gaaagcagat ttctctttta ggcgatctag ccgttacttc ctctggtcaa aacaatatcg
     3781 aagtgaacca taaaaacgct caaaaaggaa ttgctgttgc ccatgtcgct cacgagcgtg
     3841 ggatttcttt agaagaagtc atgacgattg gcgataactt caatgatgtc agcatgctgc
     3901 aagtagcagg agtcagtttc gcaatgggaa atgcagaaat cgaagtaaaa gattatgcca
     3961 aatatacaac ggacacaaac cttgaatctg gcgtaggaaa agccatattg cgcgcgattg
     4021 cagaagactt gtagttttat ctagaaaaga gggatataga tgtcagaatt tttcattcag
     4081 gaaaagcagc ttggacgtgc aactcggaca attgtcaaaa acgaaaaagg acattccctg
     4141 tttttgatgg ttggtcgctg gggaacaaag ggcgatgcat tgtctcttta tgccatgaat
     4201 ggcgatctcg ttgctcacat caagcagata agcctgacat tcggtacgcg ttttgagctg
     4261 tataaagagt tcgaaaaggt gggaaccatg cagaaaatat tcaattggcc cggtgacttt
     4321 tattatattc gtagacttca ctggacagtc caaggtgata tctataatca tcaatatcaa
     4381 atccatcatt tcaatcagca aatcatgaaa atggataaag caacactttt ttcaggagat
     4441 tactatgtat tggatgtctc aaatgatgaa gatgctccag tttgtatttg tattgctgcg
     4501 gttttagatt actggctcta ccgtaaagac aaaagtcgcg ataagcctgt ttcttaccga
     4561 cttaacttaa gttaaaaaga tgtacctgaa atgcggaaat acgcatttca ggtacatctt
     4621 tttagatatt cttttgcata tgatcataaa gatgaccact gatcatccgc tgaccgacaa
     4681 ctttgaagtc atggcgttcg tataattttt tagcctttgg atttttttca tccacactta
     4741 gaccgatgac ggagcgatta gctttcttcg ccaatttagg taatgcttct aataatcgcg
     4801 aaccgatgcc ttgtcctcta aaatcttctg atacactgat agagtccaaa taccattcgt
     4861 tgggaaacgt ttctttatca atgaacaaac gtgtagacgc atctagctga tatttctcta
     4921 agataggtac cagtggctga tcgatgattg attcttcttc atctgtataa ccgtaagcaa
     4981 ctcctgcaac tttaccgtct atttcttcta cgatacctcg tgaataactg taacgataat
     5041 ttggatcagt gattgcttct tctaaaatct ctaaagtctt ttgttcccct gtctgcttca
     5101 aaaatggcag ttccatatct tttagaatca ctaagatcaa tggggcgatt tctggagcat
     5161 cttcttttct cgcaaaacga atcatagttt cctctccttt tctccattct aacatgcaga
     5221 aaaagaaatc gcaaaatcgt tattttaaaa aatcttcaat cttttcagta atgaaataac
     5281 catgagtaac gattcttgcc gcttcagttc ggcaataagg gcagacgact ggcatttctt
     5341 tatcattatg ttcgactaca ttattgactt cttcgtatgc aaattttttt ccgcaattag
     5401 ggcatttcat tttttccatg ttatctcaac ttcctttcat ctttactctc ttagcttact
     5461 cttatattgt aaaaaagaga agcaatatcc ttcttctatg cagatatcca tacctgacgg
     5521 tcctgttctc catctattaa tcggacttta taataaggac gttcatcttt gactagaagt
     5581 gtccactctt tcacttttgc ttttttcccg tagtagctga ctgcttcctt catcgctgct
     5641 tgcggttgtt tgaactcttc cgttttttct atatcaaaaa gatgttctgc ggccttttcc
     5701 gtgtcatttc gtacggttgt tttcccagtg gatggattga ctgctatgac ttgtccttca
     5761 caaaaaaact ggtattcata tgttgcattc gtaggattct ccaaatcttt ttcaactgca
     5821 aaatgaatag cagatatgtt atgttctttt gtttcgtttt ttacgattgc tgctgctttt
     5881 ttcaatgaga tccgatattc ctggtcacta atatcatcca tgtgaaaagc ggcattgagc
     5941 agtcccgtca taccggtaat ccctattacg atgagggcag caacaccaaa catcattttt
     6001 ttcattcttc attctcctgt ttcttttttt taaagaatat caagaaatga acgaatgaaa
     6061 aatcgaatga aacgaaaaaa aagtgacaat tttgttatct ttattcgtgc agttctaatg
     6121 gcaatccgtc tggatcaaag aaaaacgtca ttttttttcc tgtaaaggta tcttctctaa
     6181 tcggttctga ttcgatccct aaggactgca attctgcaat tgtttcttca atatcgtcta
     6241 ctttgaaagc aagatgacgc aagccacaag cttctggata gtttggtcgt tgtggcgcat
     6301 tagcaattcc aaaaatctcc aattcagcac ttcctagttt cagatctaat ttatagtcat
     6361 ctcgttcttt gcggtaattt tctctgatta cttcaaatcc taatttatta acataaaaat
     6421 cacgtgattt ctgataatct gaaacaatga tggctatatg atggatcgtt gtaaggttca
     6481 tatgagcact cctattcttc gtttaagctc ggatctatat taatctccag ttccattgta
     6541 aatgtgtcag attgaaaaaa atagctgttt ttccttttta taagaaaaaa ggcaaaaaaa
     6601 acagaaagta ggtcctccaa gacactcttc ctgttcccaa tataataaat gaaagtaaga
     6661 aattatattt tcaagaaaac aaacagaaaa ttatcataac acaaattatt tataattgaa
     6721 agactagttt tttttcaaga aaagcataca atagattgtg ttagtaaata cagaacacac
     6781 tacatttagt gtcttaagga gtcctcatac atgaagtcat attttcagtt tttagctcat
     6841 cagctaaaag gctggccgca acaaaattat tatcttttct ttttcagtcg tggctgccag
     6901 gtcatgactc ttgtcaatag tcccatcact tggctgtccg ttattacatt tatcggtacc
     6961 acgcttggtg tattgtgtat tttatccatc aatgcggcaa aatcagtcaa cggtatttta
     7021 ggtattttgt ctgcgatttg ttttattatc gttgggtttt cagcaaagaa ctatttaagt
     7081 atcggtgaac agattgctta tatgatcaca ttggatatcc ccgtattgtt aagtaaagaa
     7141 tggaatcata atatggcttc aaaaatccga aaattaactg gaaaaagctg gtttatcgca
     7201 atcatcagta ctttccttgt ctatcttgtt tcaggattcc tgattggtcg ttatacagat
     7261 gatcctcggc cgtgggtaga tgccattgct ttttctatta gcttgactgc tggtatcatc
     7321 agtttcttgc gttacaacaa ccaatattac tggtggttag cttcagggct tgctcaaatg
     7381 gtcctttggt atatttcatt tacgcatgga tctgcaagtt tggcgatgtt cgtgaacagc
     7441 tctgtttact tgctgaatga tgtccttgca tttaccgttt caccttggta taaccaaaaa
     7501 gaacgtgaac gtatgcaaaa aatggaagaa gaatactaca acgcacaaca acacgcataa
     7561 aaaaactgag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata
     7621 aacggcggaa
//
LOCUS       DACECR010000115         7607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.116, whole genome shotgun sequence.
ACCESSION   DACECR010000115 DACECR010000000
VERSION     DACECR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.116"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(54..2756)
                     /locus_tag="IWO17_002486"
     CDS             complement(54..2756)
                     /locus_tag="IWO17_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0291341.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(2769..4058)
                     /locus_tag="IWO17_002487"
     CDS             complement(2769..4058)
                     /locus_tag="IWO17_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ0291342.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            4208..5254
                     /locus_tag="IWO17_002488"
     CDS             4208..5254
                     /locus_tag="IWO17_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0291343.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            5256..7409
                     /locus_tag="IWO17_002489"
     CDS             5256..7409
                     /locus_tag="IWO17_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0291344.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         2139 a         1559 c         1381 g         2528 t
ORIGIN      
        1 taaaatttct ccaccatctg aaaccgcaca cacaaatttt gttccgcctg cttctattcc
       61 gccatacatt cgttttcctc ctcgaatccg attgtccgaa tctcataagg ttggattatt
      121 gggacaaccg cttgcttcgt tggttcttca agcagattca atatgacagt ttttccattg
      181 tctttagtta tttccaattt ttctaaatga ctagacatat tgaaacctct tactactact
      241 ttatcactaa acttgctgcg tttcaaagct gtaattgcaa acgtttcact ttttatcgtt
      301 aaatattgtt gttttgaaat caatgttcct tcatgatgtt tgatttgttg agtggaaaaa
      361 ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt tttcttctgg tccatgtagt
      421 tcaatgctgt aattgaaccg atgctcacct agacactgtg cttctggtgt tggaaaatat
      481 ccccaatcgc ctagttctcc cacactgcga agtagcgtca ctgcgatttg tccgtcttct
      541 gttacttcat attcattcaa accaaagttt ccgactgtta caccgtactc ttcatcatga
      601 atattgacaa atgcttgctg atgttgcgga ttcgtggggt tttcccaact tttagacaca
      661 tggtttgggc gcttcaccac ttcaaaaata ctatctgctt cgtgatgttc cacatgtaac
      721 tttgtaggaa acaagacacg taagcgatga tctttcatct gattgtcgag agtcgtttca
      781 aagtcgattt ttttactatc ttttctgatt gtcatgattg tttttatttg aaggattcgt
      841 ttttctttgg atcgttctgc ttttcgataa cgaaattcca caaccatctg ctgttctttt
      901 tctaaacgct cgtctgcaga aacagggatt tccatttcct gtagaatact tatttttgct
      961 atttccggtg tatctactaa tacttttact tcagagtttt ctttgttact ggataggatt
     1021 ggctgatcac agaaaggttg tttaaagata tactcatttc ctatatcgcc gatatcttca
     1081 aaaatatgca gattattcaa ctttcgtttg tttttgcgat cttccattgt aatggtacca
     1141 tttttttcaa tcgtcaaatg tagagggccg ttttcgatga ttcgtccact ctgatgaatc
     1201 attgtttcct tttcttctgc tttcgcttcc ccttctacaa gcgcgaatgt ttcccaagaa
     1261 aatgctggca tgttttctaa tggtaaagtc actttgatca ttcttgccat atatggcaca
     1321 cggaaacggt ctttcggcaa atcataacca aattggacag tttcttcaga taaaatagct
     1381 ggtatagtgg ccccactttt tgtttctaca tgatacttac gtttaggcaa attttctaac
     1441 tcttgatata gttgttcagg tattccttca gcgaatcttt ttctttctaa ttcgatggtt
     1501 atttctgctt caccagtttt atccattcct gcagtattta caatgacaaa tggaaagctg
     1561 tcttttggga atccggtcgt atcgattgct gcggtcagct gttctaagga atcttgtgca
     1621 agatactttc caacttcatc tgctttttca aaacgaggta tcatttcccg atggacagaa
     1681 tcgactgaac aaccgcaaat actgtcgtga ggatgatttt gcattaatgt cttccaagca
     1741 taatctaatt gatcatgggg atagtttcca ctaacttcat aagccatcgt cgcaagtggt
     1801 tctgtaatat tttctagctg tcgctggacc ttggtattcc attgttttaa gtaaacccga
     1861 gcagatgctg tattggctaa agtataccag ccatccgttt cttgacttgt caattctcct
     1921 tctactgagc ctaagtcttc tggcacatct ttttgaactg cttccagata gtcagtaaaa
     1981 ttagaatgaa taaactcgta atctggaaac agttcatttg cgagatggat tgcttttgat
     2041 atatcttttt gtaccggttg atgatcaaca ccattcatca tcaataagtg gttcgtagag
     2101 gcatattgtt ctgcatccgc taatttttgc ttccaaaagg caagagctgc ttctttttct
     2161 actggaatct cattcccgtt gctataccaa ttggcaaata gcagaccaaa gattgctgtt
     2221 tgatcaggtc ctttccacca catctctgag tattgggaag aatagttttc tgcttccaaa
     2281 acttgattat caaagccaat tggttttaca ccacggccaa acgctgctgc tgaaatacca
     2341 gcttgtttca ttaattgagg cgtctgtccc atatttccaa atgtatcggg aaaatagcct
     2401 aacatgacag gggttcccca tttacggctt tcttccatgc caatcagcat atttctcacg
     2461 ttcgattccg agctgataag aaaatcatct tgcaagatat aaaatggtcc aattctcaat
     2521 tttccttcat tgattgcttg ttggattgcc tgtctttttt ctggccttac ttgaagatag
     2581 tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt tgaaggacgg atcaacttca
     2641 aatagctcta gtagatcgtc tatcaattca atcagtcgca tgtgatgctg ttcgtaagcc
     2701 atgtaccatt cacgatccca gtggctatga gaaatgatat atactttctt tttcatttac
     2761 gatacctcct acttttctac tctaatatca aagtaatcca tgactaattc gcaaaacatc
     2821 atatttgccc aagaaaacca ttctctcgta taattttcag gattgttcac atcgaatcct
     2881 tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta aattcaatat ccgctctttt
     2941 tcagatttgt ctgacgttgt cattccttcc atagctaatg caatcggcca aacgtatttt
     3001 tctggcgtat gggaggatcc aattcctttt gcgtactttc cttcatagaa atatggattt
     3061 tctttactca acaatgttct tcttgtttgc agataatctt cctcttgttc agaacaatat
     3121 cccaagtaag gtgcggcgat cagattgggc acattactgt catccatgac tgtggcgttt
     3181 cccattccat ctacttcata tgcataaatc gtttgccctt tttgattaac cgttcgtccg
     3241 tgattctgaa tcccctcttg gatctctttc ttcaatttct gtgtttgctg tacaatttct
     3301 ttatcatcga gtatcgtttg aaaaatttct tccacataac ctaacacgac caccgcaaac
     3361 atattggatg gtaccagata tccataacgg caagtatcat cgctaggacg gaaaccagac
     3421 catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt cattgatcag cgtatcttct
     3481 tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt catgatcttg ctctgttttg
     3541 aacacgtcca agattttttt gataccttca ataaaagatt gattgaattg atcagttttt
     3601 cctgtatttt tgtaaagaag ataggccaac tgtattgggt agcataaaga atcaatttca
     3661 tattttcttt cccagatcca atcgttcatt tttgtatgat ctgtttggtg gccagcaccg
     3721 ttagcttctt cgttgaaggc attcgcatat ggatcgatat tgatataaaa aaattgcttt
     3781 ttgactaacc cgctgatcat gtcagctaat tcctcatctt cttttgcaat cactaaataa
     3841 ggacgaactt gtgcagtaga gtctcttaac cacatcgctg gaatgtctcc cgtcaataaa
     3901 aaggtggttc cgtcattttg ctttttgact gttgtcaaca gagtattggc aaatgctgct
     3961 gtaaaattct ttgcccagtc cgcatggata tccccacatt tttgtgtgat atcttccata
     4021 aatttctgca ccgattttgg tatatcttta taagccacta ataaaacctt ctttctattt
     4081 gatatatctt taaagattat acttcataca gccaattatg tctacgcttt caaaaaatat
     4141 ctttttattt actttttcat ccattatggt attatttgaa tatgatatat cattaagagg
     4201 tgtttatatg aatcaaccat tgtaccagac aatatttaat gatttgcaaa cggctatttt
     4261 aactggtgaa ttacctgtag acagccaact accaactgaa aaagaacttt cacgtaccta
     4321 taaagtaagc agaatcactt ccaaacgtgc attgactgaa ttagagcaat taggacttat
     4381 ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct tctccggaac agattcttgc
     4441 ttctcctaca aaaacacgac ggattctgtt tcttcttcca tatgtcgctg atttgtctgt
     4501 tggtgatttt agcaagggat tgaaccctgt gatgcaagat aaacagattg atgtcatgat
     4561 gacgacgctt gactttctac aaaataaaac agcaaaagag ctaatcaatg aatttgacgg
     4621 attgatttat tatgctcttg atacagaaca acatcttgac ttactttttg agctttcatt
     4681 gaaagaattc cctgtcgtca tattagataa aaaaatctat gagttacctt ttcctaccgt
     4741 tctttccgat aatttccaag gcggtgcgct tgctgcctct tatttgatcg aacagggaca
     4801 ttcaaaaatc gcttatttgt ttggtacgca gactcatcca caatccgttc gacagcgtta
     4861 ccttggttac cttgaagcat tggacaaagc tggtttgact ttccacactt cactggatga
     4921 caaaagcgcg acattagatg aattgctttc ttatatcact aagtatgaag taactgcttt
     4981 tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat ctcttgagac agcatggtta
     5041 tcatatccct gaagattttt caattatcgg atttgatgat atccaagcag cagctcttgt
     5101 tgacccgcca ctgacaacca tcgcacaaga ttttgaacag ttaggtaagc ttgcgggaga
     5161 tgctttggtc acttggttag agacaaaaga tcgaccatcc gatagtaaat ttccagtttc
     5221 attcatcaaa agacaatcaa caaaggagaa ataaaatgaa tatcggtcaa atagatacta
     5281 gacatgcaac agcgaatcag ccaaatttct ctaatgggaa ctgccagcct tatactggcg
     5341 ttccttttgg gatgaattat tttgcaccgc aaactacgga tcaaaacggc agctggtggt
     5401 ttcatcctga tgaccgcgtt tttcaaggct accgtcttac ccatcaacca agtccttgga
     5461 tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg aaaactacag gagaacaccc
     5521 ttttccatgc tcaaagttct taccgcccag aagaaagtat attttgcccg actcatctgt
     5581 cgatctgtca attacgttat ggcatctgtt ctacattgat acctagcatg tatggcggaa
     5641 tcttatctat tgattacagc aaaaatgaca gcggtttact tttatccttt ccgggacgcc
     5701 atcagctctt tgttatagat ccttacacag tggaaggaaa aatcacacat tttgctggtt
     5761 cagaagatcc tgatttttct ttttatttta tattgagatt tgaatatcct ttaacaattg
     5821 atgagttagc tgtttccaac aaaaatagcg actcgatccc gttgtatttt gaacagacaa
     5881 aaaaacaaac tatccgattt gggacatctt ttatcagctc ggaacaagca gcttataacc
     5941 tggaactaga acaaaaccaa agcaaagaga cttaccttgc tcacagtcaa gaacaatggc
     6001 agtcctattt tgatcgtata cagctcacac accatgatca acaacaattg cggacgtttt
     6061 atcataattt atatcgggtg tttttatttc cacaaacatt ttatgaaatt gatcctagtg
     6121 ggcaaaaaat ccactatgat acgactagtc gatctataaa acctggtgtt ctctatacaa
     6181 ataatgggtt ttgggatacg tataaaacgg tttatcctct ttattctttg attgctgtag
     6241 aaaaatacga agaaatgctg gaaggttttt tgaacagcta tagagaaagt ggctatttgc
     6301 caaaatggct gtctcctgat gaacgtggac ttatgcctgg gacattgatc gatgcggtca
     6361 ttgcagatgc tgctgtaaaa gatatccgac gagacttgat gcctgaattt ctaacggcaa
     6421 tggaaaaagg tgctacggtt caaagcaaac atccaaatta tggacgccaa ggaacagaag
     6481 attacttgac atatggctat gttcctaatc actaccacga atcagtcaat cacacattag
     6541 actatgctta tagcgatttt tgtattagcc aagttgccaa ggtactagaa gaagaggaaa
     6601 tgacgaagaa atatcagcag caatcattga actatcaaaa tatctttgac tatgagtcag
     6661 gatttatgcg ttcaaaagat aaagatggca agttcagaga accattttca gctaatcgtt
     6721 ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag ctttgctgtt tatcacgatt
     6781 tttcaggact gatcggaaaa tatggtggca gtgagagata taaagaaaaa ctcattgagc
     6841 tgtgcaatca gcccccaacg tttgacccag gaggatatgg ttttgagatc cacgaaatga
     6901 gtgagatggc cgcgatagaa tttggacaat tagctatttc aaaccagcca agttttcact
     6961 atccttactt gttcagttat ataggacaac ctgaaatggc acagccactg cttaaacagt
     7021 tgatgactca gaccttcgac gattctccaa tgggttaccc tggagatgag gacaatggaa
     7081 gtatggccgc ttggtatatc ttcaatagtc tgggatttta tcctgtcacg cctggaaccg
     7141 gtgaatatgt tatcggtatg ccattaattg acaaggctgt tttgcttttg tcaaatggaa
     7201 aggaattgac gattgaagca acgcctaatc ggccgcagca acaatttatc cataaaatca
     7261 aacggatgga caaagaatat acgaaattat attttaccca tcaagatttg ctgaaaggcg
     7321 gcacgatctc ttaccaatta gggatcatcc ctcaccctaa gaaatatcaa gcaaaagatt
     7381 tgcctttttc cttatcaaac tataattaac aagaggttgt gaaaaagctg tcctgcatca
     7441 agtaataaga acagccaagc aaaaatagat cctcatattt ttcgtttggt tggtcttatt
     7501 accgcagatg caagcttttg aacaccgttt attgagaggt tgtgaaatca gcgttctgac
     7561 ctgagtatta gagagacaaa tggcaaaata gttcctcata ttttgcc
//
LOCUS       DACECR010000116         7598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.117, whole genome shotgun sequence.
ACCESSION   DACECR010000116 DACECR010000000
VERSION     DACECR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.117"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(136..426)
                     /locus_tag="IWO17_002490"
     CDS             complement(136..426)
                     /locus_tag="IWO17_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291345.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="IWO17_002491"
     CDS             complement(544..804)
                     /locus_tag="IWO17_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291346.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="IWO17_002492"
     CDS             complement(941..1354)
                     /locus_tag="IWO17_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ0291347.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="IWO17_002493"
     CDS             complement(1810..2295)
                     /locus_tag="IWO17_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291348.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="IWO17_002494"
     CDS             complement(2434..2649)
                     /locus_tag="IWO17_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ0291349.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="IWO17_002495"
     CDS             2873..3673
                     /locus_tag="IWO17_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0291350.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="IWO17_002496"
     CDS             3692..5560
                     /locus_tag="IWO17_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0291351.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="IWO17_002497"
     CDS             5553..6044
                     /locus_tag="IWO17_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ0291352.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="IWO17_002498"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="IWO17_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ0291353.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..>7598
                     /locus_tag="IWO17_002499"
     CDS             6604..>7598
                     /locus_tag="IWO17_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ0291354.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2235 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctg
//
LOCUS       DACECR010000117         7574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.118, whole genome shotgun sequence.
ACCESSION   DACECR010000117 DACECR010000000
VERSION     DACECR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.118"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(592..1938)
                     /gene="gor"
                     /locus_tag="IWO17_002500"
     CDS             complement(592..1938)
                     /gene="gor"
                     /locus_tag="IWO17_002500"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ0291355.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2072..3313)
                     /locus_tag="IWO17_002501"
     CDS             complement(2072..3313)
                     /locus_tag="IWO17_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0291356.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3331..4254)
                     /locus_tag="IWO17_002502"
     CDS             complement(3331..4254)
                     /locus_tag="IWO17_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0291357.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4475..6967)
                     /locus_tag="IWO17_002503"
     CDS             complement(4475..6967)
                     /locus_tag="IWO17_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0291358.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6974..7441)
                     /locus_tag="IWO17_002504"
     CDS             complement(6974..7441)
                     /locus_tag="IWO17_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ0291359.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2087 a         1645 c         1217 g         2625 t
ORIGIN      
        1 cgagaaactg gcttttgaac accgtttatc agaggttgta aaatcagtgt tttgtcctga
       61 gtattagaga aacaaatgga aaaatagttc ctcatatttt gccgtttttt gagctaatac
      121 cgaaggacag ctgattgaac actatttgtt cggaaaaagt gactaactat gacacaattt
      181 ttttcacagt cccttttttc acttgatttg attcgtacca gcgaacttga cgagaacttt
      241 cttggtttgg taactatatg gtattctttt aacatcctcg tttgcttctt ctaatttatt
      301 tatacaaagt cacacttttt taaacatctg ttcttcgata catttctatt tcttttaaac
      361 atcgatacca atattttgaa cttaagagtg cataccaaat ggattcattt actcttcgtc
      421 agttacgaag ataatcaaaa tctccaactt ccctacttat ttcgttttga gaattatcga
      481 agcaatctta cataaataac taaaaaacaa aaaaccagaa aatcagtata ctgattttct
      541 ggttttacat ggtttgttca ataaaaagct gttggaactg ttaatattaa attatcgcat
      601 cgttacgaat tcttccgctc ctgttggatg gatggctact gtattgtcaa agtcttcttt
      661 agttgcaccc atcttgatag ccacggcaaa accttgcagc atctcatcga caccaacacc
      721 aatagcatgt aagccgacga ttctttcttc ttttccgaca cagatcaatt tcatttcgca
      781 tttttggcga taatcattta atgcaaagta cattggggta aagcgtgaac gataaacttt
      841 caattcattt tcgccatatt tttccaatgc ttcttcttct gtcaaaccaa ttgttgcaat
      901 tggtggatgc gtaaagacga ctgtcggaac aagtttatag tcaagataag aatctttctt
      961 tccattaaat aaacgttctg ataaacgtct accagctgcg attgcaacag gagtcagatc
     1021 taatttaccg atcacatcac caacagcaaa aatatgttca tccgttgtat tttggtattt
     1081 atctactttg ataaaccctt ttttgtccaa ttccacattt gtattttcca gacctaattt
     1141 ttcggtattc ggtacacgtc caccggcaaa aagtacacaa tcaccagtta aggtttcacc
     1201 attttcaaat agcaccgtat attcgtcatt gtttttagta atttctttag caatatgatg
     1261 tgcgtaagtt tttattccag cttcttggta catttggatc aaattgtccg aaagcatttt
     1321 atcaaacgtt ctaagtggtc gttcttttcg atatgcccac atgacttcac tacctaaacc
     1381 acaaaataca cctgaaagtt cagccgcaat gtatccagca cctaaaacga ttgctctctt
     1441 aggtaactct gttaattcaa aaaatccatt tgaatcaatt gcgtattctc caccaggaat
     1501 ggtcatttta cttgcacggc ctccagtagc gatcaagatt ttaggagcac gataggtttc
     1561 accgtttact tcgatcgttt gactatcgat aaaagtagcg taacctttta tagcctcgac
     1621 atgattgcta tctaatcctc tttgataagc accgtgtaga aaatcaatat atttttctcg
     1681 tctttctact agaccggcga aatcaacatt ttttaaatct gcttggatac cgtaacttga
     1741 ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc cacataactt ttttaggtac
     1801 acaaccgacg ttgacacatg ttccgcccaa ttcattcgct tcaatcagca aaacttttgc
     1861 gccatgcatc cctgctctgt ttgctgaagc aataccgcca gagccgccac caattacgat
     1921 gtaatcatat atttgcattt tgttcctcct tattgaagag atcaatcaaa acctttattt
     1981 aacgatcctt tttctacaga aaacaagtgt aacacttttc ttgaattttg tatgcgaaga
     2041 tgcttaaaac agggatatat cagctttttc cttatttacg ttttcggatc atatctccag
     2101 gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat tggatttggt gctcggtcaa
     2161 tggattcgcc ttcttcatta tgcataactt caacggtttg actgaaatga ctgaatcctg
     2221 gaccataaaa ttcgatttca tctcctacag aaaacaagtt tctctgacgt atcgtagcaa
     2281 tctgtgttgc ctcatcataa gataatactt caccgacaaa tttatatact gggattttac
     2341 gacgttcacc gaataattgt tcgttttcag ttggtgtatt gtagtaaaaa cctgtagcta
     2401 attctcgttg tgctactttc cataactcgt cgatccattc ttgcttgcag acatagtttt
     2461 caggatcttc catataactg tctacagccg ctttatagac atttgacacc gtagatacat
     2521 aatggatcga tttcattctt ccttcgatct tgaagctgtc tacgccgttt tcaatcaatt
     2581 ctggaatatg ttcgatcatt gacatatcca ctgcactcat cgagaattct tcttccactt
     2641 cgcctgtatc agttaaactg tttcgttctt gaccaaaagg catatcgaac aatccatact
     2701 tccagcgaca agattgagaa catccgccac ggtttgcatc ccgcatcgac atatggttgg
     2761 acaatgtgca tcgaccagaa taagagatac acatggcacc atggataaat gcttcgattt
     2821 cggtatccgt atttttacga atctctgcca cttcctccat tgaaacttca cgagctaaaa
     2881 cgacacgttc caacccttct tctttccaga attccaatgt ctcaaaatta gttgctgaag
     2941 cttgagtgga taaatggatc ggcagacctg gggcttctgt cgcacaaatt tcgatcaaag
     3001 ctggatcaga aacaatcact gcagaaatac cgacatcacg caaactgcgg aaaaattccc
     3061 ctgctccatc ttcatttcct tcatgggtga ccatattagc agcaacgtat accttcgcat
     3121 catgttcttt tgcaaaagca actccttcag ccatttcttc ataagtaaaa ttacctgcac
     3181 ggcttcttaa accatatgca ttcccgccaa tatatactgc atctgctcca taatggatcg
     3241 cggtttttaa cttttccagt gttcctgctg gcgctaggat ttctggtcgt ttcaattttc
     3301 tttttactgt catttctttc atcatactcc ttatttgata gcagaaggat caatataaaa
     3361 gaatcctgta tctaattcac ggttttcagg atgcaattta ctgatttcgc tagtcatttt
     3421 ttccgctttt tcttgatccc agctaccttc taaaatttgt ttttttgctt cggcgaatag
     3481 tcgagtgatg tttacgaatt tttcgcctgg agtaaataat ccgtccaatt tccatgtaca
     3541 gtaatgatgc tgataaagtt cattcaattc attcgagaga ttcagatcat tgctggcaaa
     3601 aatatgtgtg ccatgactat cttcataaat agaataatgt gtctcttctt ttttaggttc
     3661 cgagataaaa agacctcttt cacgatcttt ttgttcgtct tgtttcgtat agttatagta
     3721 attctgtagc agtggacgtt tggattgatg gatgcatgta gcaccataca caagggtttc
     3781 tacaggaata tcgagttttt cttccatcgc cttcatctct tcaaaaggaa cttcacgagc
     3841 aagtactgct ccaactgcgc cttttttcgc ccagaaattg atttgacgtg cgctcgttac
     3901 taatgtttca gcatcataaa tatacggtat acgcaattca ggattttttt tcatcgtata
     3961 aataatacct gtatcaccaa ctgtgatcac atctacgttt atttctttta aaaaagccaa
     4021 atattcaggt acatttttca ttttttcagg atgaatgatc ccatttacag caatattcgc
     4081 ttttttacca tacttgtgtg ctaattctac caattgtcgc tgttcttctc gtgtaaatga
     4141 agcaggcaga cgcaatccaa attgctcttc accgaaatat agtgtatctg cacctgcagc
     4201 aagcaactgt tctgcttgtt caatagtttc tactgtggtt atgagttcga tcataatttt
     4261 ctcctcctac aacttcataa tagtagcata gctctaaaaa ctttccaata cttcttctat
     4321 ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa aataaagttc gatatattct
     4381 gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac aacctcttaa tcaagtctta
     4441 ctattataaa aattcagcta cacaggccaa gtaactatag ggaacctact gtttcctttt
     4501 taggtccgcg aacattcaat gtgatcttac cttttgaagc acctactgtt acgtggtcac
     4561 ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg atcttcaatt tctttttgaa
     4621 gtgcgcgacg gattggacgt gcaccatatt caggatcaaa tccagctttc ccgatcacat
     4681 cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc tttcaatcgt ttgataatcg
     4741 ctttgctcat gatttggacg attgtatgga tttcatcttt actcaatgaa tggaagacga
     4801 cagtctcatc gattcgattc aagaattcag gtcggaaagc tttctttaac tcttctaaga
     4861 tacgtttttg catggctttg tgatctttag taacatcttg gacattgaat ccgacatttt
     4921 tttcttctcg aatttgtgtc gctccgatat ttgaagtcat aatcatgatt gtatttctaa
     4981 agtccacttt tctaccttta gaatctgtta aatgaccatc atccaatact tgcagaagta
     5041 aattgaaaac gtctggatgt gctttctcca cttcatctaa gagaatcact gaatatggtt
     5101 tttgacgaat tttttctgtc aactgtccac cttcatcata ccctacgtat cctggaggag
     5161 acccgattaa tcggcttgta ctgtattttt ccataaattc tgacatatca acacggatca
     5221 gggcatcttc acttccaaac atgacttctg aaagtgcttt ggccaattct gtttttccga
     5281 caccagttgg tccaaggaac atgaatgaac caattggacg atcaggatct ttcaatccgc
     5341 ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc ctcatcttga ccaacgacac
     5401 gttcatgcag aagtccttcc agttctaaca agcgttcgct ttcttttttc tccaactgct
     5461 gtagagggac accagtccac tccgatacaa ctgtagcaac atcttcttct gttacacgat
     5521 cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac tttttgcagc tttttacgta
     5581 gtgctttttc ttgtctgcgt aattgagcag cattttcaaa gtcttgtttt tggatcgctg
     5641 cttccttttc ttgtactagt tcatcgattt ctagtttgat cacagttgat tttgttaagt
     5701 gatcggtttg atccaaacgg actttcgctg ctgattcatc gatcaaatca attgctttat
     5761 cgggtagttg acgagaagtg atataacgaa cagaaagatt gactgctgct cgtacagctt
     5821 catctgtgat ctccactcca tggtgctctt catatcttgt acgtaatcct tgaagaatca
     5881 cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg agcaaaacgt cgctctaatg
     5941 cagaatcttt ttcgatatat ttttgatatt catctagggt cgttgctccg atcgtttgta
     6001 attctcctcg agctaacgcc ggtttcaaga tattcgaagc atcaattgct ccttctgctc
     6061 cacctgcacc aattaaggta tgcaattcat caatgaataa gattacttga ccatcattat
     6121 agatttcgtc gatgaccttt ttcattcgat cttcaaattc accgcgatat ttcgtaccag
     6181 caaccaaaga tcccatatca agcatcatca ggcgcttttc ttgcatgtct tctggcactt
     6241 cacctaaaat gattttttga gctaaccctt cagcaatggc tgttttgcct acaccaggtt
     6301 cacccaccag tacagggtta ttctttgtcc gtcggctaag aatctggata agccgtttca
     6361 cttctctcga acgaccaaca actgggtcca gcttgttttc ttttgctaat ttcgtcaaat
     6421 cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt ttgccgttta tcttgtacat
     6481 ttacatttct acgacgattc attccttttc cagcagaatc accagctagc cccatttttt
     6541 gtttcagaac ttgacgggct tttgaaaggc tgatacctaa gttgatcatg attcgagtag
     6601 ccatgatact ttcatctttt aataagctca tcaaaatatg ttctgttcca actttttgag
     6661 aaccagtttt cttcacttcg ctgctagcta aagcaaaaac ttgtttcatt cttggtgaat
     6721 atggaagaaa gactccttta gggtaagaac ggataccacc ataaccaatc agatgttcaa
     6781 tttcttcata aatttccgtt tcattcaaat ccatttcacg taatactttt ccagcaattc
     6841 cattttgctc gaccactaag gctagtaaaa catgttctga actaactgat tgatgtttca
     6901 accgttttgc ttcttcttgt gcaatttcta atactgcttg ggctctttca gtaaaaagtt
     6961 cttccatcta atctcacttc ccatcttcat aacttaaacg ttccagaaat gctcggagaa
     7021 tccgagcacg tgtatgatct tcatattcat tgaaattcaa tgtgtttttt gcaattgcac
     7081 tcaacatcaa gtttccttct ttttttgtaa ttatttggtc ttcataaagt ttttgaataa
     7141 tagcaaaagc atttttctca ctgattgtat catcaaataa ctgatttatc tgctcaagta
     7201 attgtttttt atctgaaatc cttacttttg caattcgtat ataaccacca ccaccacgtt
     7261 tgctttctac tagataacca cgttggatgg taaaacgggt attaatcaca tagttgattt
     7321 gtgaaggaac acagttaaag aggtctgcca tttcaatcct tcgaatctcg atcatttcac
     7381 tattttccag aattttcttt atatatgatt caattaaatc cgaggtattc tgatgtgcca
     7441 tcttattcat tccctttgac taatattgac cttaattata acgaatttga taatagaaag
     7501 aaagaagaac gctttttgtt aaataaatcc tttagaatca acaaaaaaaa atacggaata
     7561 tactattccg tatc
//
LOCUS       DACECR010000118         7530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.119, whole genome shotgun sequence.
ACCESSION   DACECR010000118 DACECR010000000
VERSION     DACECR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.119"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            34..1175
                     /locus_tag="IWO17_002505"
     CDS             join(34..262,262..1175)
                     /locus_tag="IWO17_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0291360.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1410..1862
                     /locus_tag="IWO17_002506"
     CDS             1410..1862
                     /locus_tag="IWO17_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291361.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1876..2565
                     /locus_tag="IWO17_002507"
     CDS             1876..2565
                     /locus_tag="IWO17_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291362.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2690..5041
                     /locus_tag="IWO17_002508"
     CDS             2690..5041
                     /locus_tag="IWO17_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ0291363.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5121..5516
                     /locus_tag="IWO17_002509"
     CDS             5121..5516
                     /locus_tag="IWO17_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291364.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5841..6458
                     /locus_tag="IWO17_002510"
     CDS             5841..6458
                     /locus_tag="IWO17_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0291365.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6499..6798
                     /locus_tag="IWO17_002511"
     CDS             6499..6798
                     /locus_tag="IWO17_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291366.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6832..6999
                     /locus_tag="IWO17_002512"
     CDS             6832..6999
                     /locus_tag="IWO17_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0291367.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7044..7514)
                     /locus_tag="IWO17_002513"
     CDS             complement(7044..7514)
                     /locus_tag="IWO17_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0291368.1"
                     /translation="MEFREGAFDFQTSQQNPSLDRLGRDYEDIKNTVAFMKQKKVALK
                     ILDAQFLDFNTGNELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKG
                     RPIEYSINSTDPQKRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2591 a         1139 c         1509 g         2291 t
ORIGIN      
        1 gaatctaact actaagaaaa ggacgtgtcc acgatggcaa aagccaagaa aaaatttgat
       61 gaagacttta agaagatgat cttagattta aaccagtctg gccaatcggt cgaagaactg
      121 gcagcgcaat atggtatcgc tacacaaacg atttatcgtt ggaagaagct tcatacaaag
      181 aatgtagcaa ccggcatgac tgaggcagaa atcctagcga tgaaaaagga aatggcccgc
      241 atgcaggagg aaaacactat cctaaaaaag gctttaacca tattcgctca aaagtaaaaa
      301 tgagtgatgt cttcgaattt attcaatcaa atgttcatga tcatgacgta aaacaaatgt
      361 gtaccgtttt ggaagcaccg aaatccagct actatgactg gaagaaaaag aaaccttcca
      421 agcgccaatc agaaaacaaa cgcttaaacc gcttgatttc aggaatctac tttgaaaaca
      481 agggtatcta tggtgcgcct aaaattcata agattcttgt tggccgtggc gagaccttgt
      541 ccttaaagaa agtacaaata ctgatgagaa aattaggctt gcgttcagtc accctcaaga
      601 agtacaagcc tggcaaacaa gcgaaagtta aaagtgaagg acgtaaaaac ctgttaaacc
      661 aagattttac cactacgtcg atcaatcaaa aatgggtgac agatattacc tatattcata
      721 ccctaaaaga tggctggacc tacctctcca ctattcaaga cctccacaca aagaaagtca
      781 tcggttggaa gtttggcaag cagatgacga aagaattggt tatcgaaacc cttgacaatg
      841 cgctcttaaa ccaaacaccc tccgagaact tgatcatcca ctcagatttg ggttctcagt
      901 acactagcga agcctatgag gcaaagctga atgaattgaa cattcggcac tccttcagtc
      961 gtaagggctg tccctacgat aatgccggta tcgaatcgtt tcacgcctca ctcaaaaaag
     1021 aagaagtcta tccatcaaaa gcgtatgaaa actttgaagc tgcttattta gcgttatttc
     1081 aatacattga aggattttac aatcgcaagc gaatccatag tagtatcaac tatttaacac
     1141 caaatcagat ggaggagttg gcattgcaag cttagttcct agctaccact tacatttttt
     1201 tggcgagatt tccacagggt ttcaacttca tcgcttcgat cagcggtcaa acggttttgg
     1261 tttgaccgct ggtcgaaacg atgagatact cacccgctgg aatctcgaaa aaattctcac
     1321 aaatcgtgtc cgaggtattg actcaaatcc accaattatc ttatcgtaga tatatattat
     1381 aaatttaata agaaagcgag gtgagaatcg tgaaaaaaac gatactaagt attttttcag
     1441 tatttacaat attaattggg ggctattttc ttttgaattt agattctgtt aattcaaacg
     1501 ctcaaataaa agatcaaaga gtagctgaac ataataataa aaaaatgagt ttgaccaaaa
     1561 atatctattc tatgtcagat gaagaattgt ataaagaatt tggtgattta tccggggaac
     1621 agttagaaga aagtattcca ggaattagtg actcagataa aattgttgtc ttgtctgctg
     1681 gtgggcaatt aatgggagat ggcaaatccg aattagttag ttataaacaa aatcacagac
     1741 agtttattga tgttaatact gattccaacg ctatttcgat taaggatcta aaagagcttt
     1801 taaatagtaa aagaaatgaa ttaaagcaga taaaagaaac taattcagtg gaattagagt
     1861 aaaggaagat tcattatgaa aaagattttt ggtgtttctg ttgggctact tatgatgtta
     1921 acatttggaa atgcagtata tgctgctgag aaagaagtta tttcagatcc agagcagtac
     1981 gcctcacgat actttgctga ttttgaagaa gaagataaag tacttgcatt gccagaaggt
     2041 ggttttttaa aaggggaagc taaaattgta tctatggata atccagatat aattcaaggt
     2101 tcatataatt cagaaactga tcctgcttca gtttcagtga cagaagcaaa aaaaattgtt
     2161 gctgaaaaat tcaaaaatga tattgaaact actccaatta tgcctcgagg agcatttcca
     2221 cccgatgttg gtactagcgg acaccttctt gtactagcag gaggaggaag ctataaatca
     2281 cagccattta gtggtgcggg atggcgtttt ggaggtatgg gtgtaaaacc agctgatggt
     2341 acaggcaact ttttacgttg gaaatcctac attgacgatg gaagagtagg aaatgtaaat
     2401 caagccaacg caactaaatc tggttctatt caaggaatgg cattgtatgc tcgtgaagga
     2461 ttcaaatact ttgcaccaaa cgggcctggt tggacaatga atataggtat ttattacact
     2521 tataatcctg ttgcaggtac gtattatgtt gttcaaaata tttaataaaa cagcacaaag
     2581 agatttcttt gtgctgtttt attattttta gttgttgaca aacactaaaa tataaaatat
     2641 aatcaacaca taaacgaacg tatgttcggc aagaaagcag gggattttta tggatgagat
     2701 ggttttaagt actcagaaat ggctaaacaa gaaatacagt aatgttacag ggtttgataa
     2761 ggttccagaa aatgggcgaa cagggtggcc tacaatctat ggacttattg aaggactaca
     2821 agtagaactt ggtattacta atctagtagc taattttgga cctaccactg aaaaaatgta
     2881 cgataatcag gttactccta aatggggcaa aaatctacct aaaaatattg tatttcttat
     2941 tcaaggtgcc ttttggtgta aggggattaa tccaggtggc ttcgatgggg tatatactcc
     3001 atatttagat actgctgtaa aagagttgca gactgatgca gggtttaatg gaagcgctgt
     3061 aactggagtt ttggatgcga aatgggccaa agcgttattt gatatgagtg cttttgttct
     3121 tgttcccggc ggagatagta aaattagaaa aatgcagcaa tggttaaatg taaattattt
     3181 agaatacacg ggaataatgc cttgtgatgg tatttatcaa agaaattcaa atcaagcatt
     3241 aatttttgca ttacaagctg aactaggata ttctcccagt gaagcaacag ggtcttatgg
     3301 taatgggaca actagtaaaa cttatcctgt gtctgaaggt aactcgggaa actatgtaag
     3361 aataattcaa tatggattat atgttaatgg ttattttgaa gacggtacat tcgatggtat
     3421 tttcactaaa tatatgggtt tagaagtact ggcattccga aaatttatgg tattgcctga
     3481 atatactgaa atagcagatg ttgttgtaat aaaaggacta ttgtctagcg ctggagatat
     3541 tagaagatct gcagatggcg cagatacttc tacgcaatta actagatctc aaatccaaac
     3601 attggttgat aatgatataa aaatagttgg tagatatctt actggtactg ttggaattgg
     3661 aaaagatgaa cgaaataagt atctaactac agaggaattg aataatattt tttctaaaaa
     3721 tttatctgtg tttcctatct atcaagatgg aggtgcagca ttagcttatt ttacttatga
     3781 tcgtggctta tcagatggta agaaggctat agatgcagca aaaaatctaa atataccatt
     3841 aacaacagtt atttattttg ctgttgatct agacatgtta ggggaagaaa ttcttgcata
     3901 tgctggagaa tattttaagg gggtatctgc tgtaatgtca tatagcgggt atcaaacggg
     3961 tgtttacgga acaagaaatg tttgttctca aataatcaat aacgggtatg cttcgtttgc
     4021 atttgtttca gatatgtcga ctggttatag tgggaattta ggttttccta tgcctagaca
     4081 atggtctttt gatcaatttg ttgaatttac cataggaagt ggcaacggag ctgttggtat
     4141 cgatttaatc gctacatcgg gtagagatag cggatttaat gagttatcaa atgatactaa
     4201 taatgataat tacattgcca aatacaatca gaaggttata aatcagatgg tacgtgcgta
     4261 tcaatatcta tcacaagctg gtcttgataa tccttggaac cctcatttga cattttatag
     4321 atatgtaaat tattctggtt tgtcatggga catcatttct tctcctgtaa cagagcatga
     4381 tagaaaaatt tatgatgaat ataggaataa attaactaat agcgagggcc tgtataatta
     4441 ttttatagat cctaatactg gttcaataat aggtttgcct cacttaattg tgacgttaca
     4501 atcacagatg tttctagccg acacaatcaa tgatagtgtt tctgactttg caggttggtt
     4561 aggagattta atgacttgct ggggtgaagt taagaaatta ggaatacaaa tggagcaagg
     4621 tgtatttgca ttagtaggta cggctgcagg tggaacattt agtctagaag atttatacca
     4681 agatgttgac gcggtaaatg tttatacctt agcaaaaaga aattctgatt caacagcttt
     4741 atcggcttta aaaacgtact attgtgataa gggtgctatt gatagattta atcaatttgt
     4801 taagaacaga tttggttctt cagataaaat cgcttcagta acaaatagtt tattagaaaa
     4861 tgacgaaaca gttgatatct taggtgtagg gataatttca tttttcattg ataaaatagt
     4921 taataattca cttgggacag attttactta tcttgatttt attggagatg aggggaaagt
     4981 tgcaaatgga ttttctgaaa aaattattca atttcaagaa aatagaagta aacttaatta
     5041 agaatcgttt actatttaaa cagtgtcctt ttccttttta aatatatacg ataaaataag
     5101 tatatgaatt gtaaaaggac atgatgatta tggaagaaat agaatggaat aatctgactc
     5161 aaaataataa aaataattct ggatgtggaa ttatctttat gtttgttata gttttgggaa
     5221 tagtagtact tttaataact gtcaagatag tagattactt tgcgaatact cctgaagaac
     5281 aaatacagag aaaacttcct gagtttgaaa gagtaccacc ttatgatcca agagataatt
     5341 catttcaagt aaaaaaatta aaagagtctc caaatgaaat gtggtttgaa gttacagttg
     5401 aatcaagcga cttcaaagaa aaaaagaaag aatacaatta tattgtaaag gcaaaagtat
     5461 caaaaagatt tatttttaac cctaaattag aaatatatag tgttgtaata gaataatagt
     5521 gttaaaaaaa ttgtaaaagt taaagaagtc aaattctgat gtgaaaaata aaatatacca
     5581 tggaagacat aaaagaaaaa ctggataact agtttattta atggcagacc gttaaggcct
     5641 gccattttta tgtattttag cggaataaat ataatcatgc gggagtgtaa acttactgtt
     5701 tcaaaaagta tactttttga catgccttga ttttctttgt ttatgtatgg cataataacc
     5761 acataataca atttgaaacg tttctgattt aatctgattg ttttgaaaca attttagtga
     5821 aaaaagggta gataaaaacg atgagaaaat ttttttatat ccgggtcagt agttatgatc
     5881 aatcgatcga acgacaactg atttccgcga aggaattagg aatccctgaa gaatatatat
     5941 ttgtagaaaa agccagcggc aaagatttta aacgccctga atatcaatta atgaaacgga
     6001 tgtttcgaaa aggagattta ctctatctac aatcattaga tagactaggc agaaataaac
     6061 aaatgatttt agaagaatgg cgagaattaa ctaaggaaaa gcagattgat atttctgtgt
     6121 tagacatgcc tctgttagat actgctagtt acaagcaact agatgggtta gagacgctta
     6181 tctctgactt aattctgcaa ttactcagct atatggccga agacgagcgg aaacgaatta
     6241 atagccggca aagagaaggc gtagaagctg cgaaacaaaa gggtgtaaaa ttcggcagaa
     6301 agaaaattgc tttacctcct gaatttcctg cgatctattc tgactggaaa gcaggaaaga
     6361 taactgcagt ctatgctatg gatcaattgg gcatgaaacg aaataccttt tatcgacggg
     6421 tgaaagaata tgaacatacc ttgcaccact tttcttgata gatataacag ctattagacg
     6481 ggaagggaga gtttgaaaat ggacttaacg aatcaaaggg tcgtttttac tggaacgctg
     6541 cagcaaatga cacgaaccca agccatcggt ttggttcact ctttaaacgg ccattgtcaa
     6601 tcaagtgtta cttcaaaaac aaatttcctg gtttgtggtc agtattctaa gtcgttgttt
     6661 gatgattctc tctatacaaa aaaggaacaa accgcgcggg acttgattgc gttaggtcat
     6721 gagattacca ttttatcaga agtcgaattt tatgcgttaa ttagtcaaca gttgaaagaa
     6781 tggcaacggt atttataatc gttagtaaaa tttacaagga ggaaaatttt tatgaagcga
     6841 aaatcttatg atcgttgggg cattgaaata actagtttta atcgaaatat tgcttttcac
     6901 gtggagtgtg actgtgggga attagcaaaa agtgtttatc aaaaaaatta cttttactgt
     6961 cctaaatgcc atcgccgata cggggtacat ggaacataga aacgaaatag tgcggtagaa
     7021 aagcgaatga atgtagtata tttctacaaa tcattcgctt tctttatttt ataaatagtt
     7081 ggtcgagaga tcccgttttc ttttgcaatc tctgcaactg gaatgccttg ctttaacatg
     7141 tccaccacgg ttttaaaaac taagcgtttt tgcggatctg ttgagttaat agaatactcg
     7201 attggtcgtc ccttatatcg gccggctttt tgagccaata atactccttg ccgctgacgt
     7261 tcacgaattt tctcacgctc gttttgtgca atataactta gcagtgataa aaacatatcg
     7321 aacatagcgg tatccaggag ttcatttcca gtattaaaat ctaaaaactg tgcatctaat
     7381 atttttaacg ctactttttt ctgtttcata aaagcaactg tattttttat atcttcataa
     7441 tctcttccta aacgatctag tgatgggttc tgttgcgaag tttgaaaatc aaacgcgccc
     7501 tctctgaact ccaaatatct taggctgtta
//
LOCUS       DACECR010000119         7511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.120, whole genome shotgun sequence.
ACCESSION   DACECR010000119 DACECR010000000
VERSION     DACECR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.120"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(2..2167)
                     /locus_tag="IWO17_002514"
     CDS             complement(2..2167)
                     /locus_tag="IWO17_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0291369.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            2427..4721
                     /locus_tag="IWO17_002515"
     CDS             2427..4721
                     /locus_tag="IWO17_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0291370.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            4714..5379
                     /gene="pgmB"
                     /locus_tag="IWO17_002516"
     CDS             4714..5379
                     /gene="pgmB"
                     /locus_tag="IWO17_002516"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0291371.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            5372..6379
                     /locus_tag="IWO17_002517"
     CDS             5372..6379
                     /locus_tag="IWO17_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0291372.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            6401..7420
                     /locus_tag="IWO17_002518"
     CDS             6401..7420
                     /locus_tag="IWO17_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0291373.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         2340 a         1639 c         1410 g         2122 t
ORIGIN      
        1 cttacatttt aatggagccg atcactttac cagctgtcac tgtacctaat tgcttgatca
       61 ctacttcttc tactttatcg tttgtaaata cgatcatgat cgttggatct ttttcttcgt
      121 tttggatcac atcaagatcc atctcagcta atttcgttcc tgttcctact tcttggcctt
      181 ctttgacagc aatctcaaaa gctggctgat tcatttggac tgtatcgatt cccatatgga
      241 tcaaaacttc gataccatct ttcgtttcca gcccaattgc atgtttgcta gggaaaatac
      301 ttgtcacttt tccagcgatc ggcgcaacaa cttctcttga tgttggaata acagcaaagc
      361 cgtcacccat cattttttgt gagaataccg gatcttttac ttcagataaa ggaatcactt
      421 ctccagaagc tgcggaaact agttcttgtt ctatacctaa gtattgctta ctacctgctt
      481 gtccatttcc tgtagaaacc atttctggct ctggaatctc tgctcctgat tgcagcagat
      541 cttcgatatc tgatttcaag acatctgctt ttggtccata aactgcttgg actccattgt
      601 ctttgacgat caatcccatc gcccctgctc gtttccaggc ttcttcagtc cctacttttt
      661 ctcgatcatt tacacttacg cgtaaccgag tcatacaagc atcgacatct ttgatatttt
      721 cttttcctcc cagcaaatga atgatctgga ccacttgctg atctgctgtt cctgttccgc
      781 tgttagatac aacggtttct tcactgtcat tgtcgtaatt tccgttacgt cccggtgtag
      841 caaagttgaa ttttttgatc aagaaatttg ctaagaaata cgtaacaaca ccaaaagcga
      901 tcacacacaa cacgaagttg ataagatctc cacctagacc cgctttgata gctaacggtg
      961 tccgagtcag caattcgata ttcccaaatg aatggacccg caatggcaag atatctgcca
     1021 tcgcaaatgc agccccttgg atcactgaat aaatgacata taatggcaca gcagcaaaca
     1081 taaacatgaa ctccaatggt tctgttacac ctgttagaaa gacagccaac gctgctgaaa
     1141 aatacattga tttatatctt gctttcttat caggatcgac attacgatac atcgcaaaag
     1201 ccattcccat caagatacct gaagaaccaa tcatttgtcc tactttgaaa cgagccggcg
     1261 tccagttatg caacacaaac tcatattgcg acatatcccc tgcaccttta agattcacta
     1321 aatcagtggc ccaagccaac cataacggat cttgcccaaa tacttgtgtc cccgcctgtg
     1381 cgcctgataa aatctcatac gatccgccaa gctgtgtata gttgatagga atcgtcagca
     1441 tatgatgcaa tccaaatggc aatagtaaac gttctaatgt tccaaataaa aacggtgcta
     1501 gaatcggtgc gctttcttgt gattctgcaa tccataaacc aaaattattg attccagcct
     1561 gtacgtttgg ccacaccaca gcaagaatca atgccacgat tgttgaccat aagataacaa
     1621 caaacggaac aaagcgcttt ccattgaaaa atgacaaagc atccggcaat tttctataat
     1681 tatagtactt attataagcc attgccccga caaatccagc gatgatccct acgaatactc
     1741 ccatattcaa agctggtgct tccaatacac ttgtaaaaaa tcctttaacc atgatcttcg
     1801 taccgaacag agtgtgggta aatgcatctt cattcgcaag catgtcactt gtgacaccaa
     1861 agatagcccc agtgatccga ttgattaaaa taaatgagat cccagctgca aatgctccgc
     1921 ctgctctttc ttttgcccaa cttcctccta ttgctaaagc aaataaaata tgaagattac
     1981 caataattgc ccaaccaata ttttcaatta ctccgcccgt tgtgacaagc ggcgtccaat
     2041 caggattgat caagggaatt gatttcccta tactgatcat caacccagct gccggcataa
     2101 ctgctaccac aaccattaaa gcctttccaa atttttgcca aaactcaaaa ctaaatatct
     2161 ttttcattcc gtttagctcc tttacgcaat cggtttcgtt aaacgtatta tagcgtgcgt
     2221 tttctttttt gtaaatgctt tattttttat ttttctgtct ataccattct ataaaaccta
     2281 taatacgtgt gttcccttct ataattttct cctaaaaatt tattttttta agcttttttc
     2341 tatgaaatat tttcttttgt ctattgactt gtgcaatcgg ttgcgtttat tatgaacgta
     2401 taaaacagct cattaggagg agaaaaatga aacagatcaa acgactattt gctattgatc
     2461 catggaaaat cagcacgaca caattagata aaaaaaatct tcgactgcaa gaatctttaa
     2521 caagtattgg aaacggttat atggggatgc gcgggaactt tgaagaaact tactcagggg
     2581 accaccatca aggtacttat ttggcgggtg tctggtatcc cgataagaca cgtgtcggct
     2641 ggtggaaaaa tggttatcca gaatactttg gaaaagtgat caacgccttg aactttatcg
     2701 cgatagatat ttcgatcaac gatcagctga tcgacttggc ttctttggaa ccagaagact
     2761 tttactggga attagatatg aaaaatggtg tattatctcg tacattcaca atcactactt
     2821 ctactaataa agtacgcatt tccttcgaac gttttctgag cattgtcaaa aaagaatcag
     2881 cttatatcta tttaactata gaaatgcttg aaggtagcgg aacagtcaaa ctcgtatcaa
     2941 aattggatgg aaatgttcaa aacgaagaca gcaactatga tgaacatttt tggaatgaga
     3001 ttgatcgtgg aactatagaa gaaatcggct atttgacgac taaaactatt ccaaacaact
     3061 ttgggattga acaatttact gttactgcag caatgcgcca tttcgttaat ggaaaatcag
     3121 tcatccctga ctatcacgaa gatactttag cattacatgg ttgcttgtct gcagaattat
     3181 ctcaaggtga gacatggtca gtggaaaaag aaattatcgt tcttactagt agagatattc
     3241 cagaagcgca gcaagcagaa aaagctgtct cagaattaac gaaccagaca aacaattata
     3301 tccaagcaaa agaagagcaa acagcggctt gggcagaacg atggaagctt gccgatgtgg
     3361 tcatcgaagg agacgaagaa gcacaacaag gtattcgatt caatttgttc caactattct
     3421 ctacttacta cggcgaagat gaacgattga atatcggccc caaaggattc acaggagaaa
     3481 aatacggcgg agccacttac tgggatacag aagcgtatgc tgtccctctt tatcttgctt
     3541 tagccaaacc tgaagtaact aagaatctgc tgaaatatcg gcataatcaa ctgccgcaag
     3601 caatccacaa cgctcagcaa caaggattaa aaggcgcgct ttatcctatg gtcaccttca
     3661 ctggtgtcga atgccataac gagtgggaaa tcacattcga ggagattcac cgaaatggcg
     3721 cgattgccta tgcaatctac aattatacga attacaccgg cgatacggaa tatttgaaaa
     3781 atgaaggact ggaagtttta gcagaaatcg cacgcttctg ggcagaccgt gtccattttt
     3841 caaaacgagc aaataaatac atgatccacg gtgtgaccgg cccaaacgaa tatgagaaca
     3901 atatcaataa caattggtat acaaatacga tcgcagcttg ggtcttgcgt tacacgcgag
     3961 aaaattattt aacttatcaa aaagaagctg ctatcactat cagcgaagaa gaactagcca
     4021 aatggaaaga tatcgcagag aacatgtact tcccagttga cgaagaatta ggaatctttg
     4081 tccagcatga cacattctta gataaagatc tcatgcctgt atccgatatt cccacaagcg
     4141 atcttcctct gaatcagcat tggtcttggg acaaaatcct tagatcttgc ttcatcaagc
     4201 aggcagatgt actacagggg atttatttct tcaatgatac ttttacaaaa gaagaaaagc
     4261 aaaaaaactt tgatttctat gaaccaatga ctgtccatga gtcttccctt tcgccaagta
     4321 tccattcgat tttagcagca gaattaggaa tggaaagcaa agctgtagaa atgtaccagc
     4381 gaacagctcg cttggatttg gataattaca ataacgacac agaagacggc ttgcacatca
     4441 cttccatgac tggtagctgg ttgtcgattg tccaaggatt tgctcaaatg aaaactgccc
     4501 atgatcagtt gagattcgca ccatttctgc caaaaacttg gaaagcttat tctttccata
     4561 tcaattatcg cggacgttta ttgttcatct cagtcacaga tgaacaagtt actttcgagt
     4621 tattagaagg ttctcctcta gaaatttacg tctatgaaca gtcttattct ttagcggatc
     4681 gtctagaaat aacgctaaga aaggaggaag caaatgttta aaggggtatt gtttgattta
     4741 gacggtgtca ttaccgatac tgctgaattc cattatcgtg cttggcgaaa gcttggtcag
     4801 gaaatcggca ttacgatcga ccacacattc aacgaacagc taaaaggcgt cagccgagaa
     4861 gactcactct ctttgctttt agcttatggc ggaaaagaac actctttttc aaaggaagaa
     4921 tttgcagaat tagcgaaaag aaaaaacgat tattatttag agatgatcca gacgatcgaa
     4981 ccaaaagatg tcttccccgg ggtcgtgcct ttactggatt ctttaaaaga agaaaaaatc
     5041 aagattgctc ttgcttctgc aagtaaaaac gggccattcc tacttgaaaa aatgggtctt
     5101 acaccttact ttgatgctat tgccgacccg gcagaagcaa caaacggaaa acctgcgcca
     5161 gatatcttcc tccttgcagc aaaagctgtc ggactaaaag cagaagaatg tatcgggatc
     5221 gaagacgcgc aagccggtat ccaagccatc ctctcaagtg gcgctcaacc aattggcgtg
     5281 ggacgtgctg aagaccttgg agaggagatc ccgcttgtcc ctgatacgac ttttcttacg
     5341 atcgattact tgaaagaaaa gtggcatgac catggataat ccagagatct ccgtcgtacc
     5401 agttccagag actgaaataa agaaaatcac attgaagaac gactacttag aagtcgttct
     5461 tctcaattac ggtgcccgcc tgcaccaaat cttcgcacca gacaaagaag gaaaatcaga
     5521 aaatgtttta ttgtcttacg atcgtttcga agatgttcta acagacaaaa gcttttttgg
     5581 agcaactgtc ggacctgtag ccggaaggat ccgggatgct tcttggggtg agcatagatt
     5641 ggagaaaaac tgcggatcac accatatcca cggtggcacg aaaggctggt ctttccaatt
     5701 ttgggaagta gaagttttca aaactccatc atcaataggt gtggtctttt acttaaaaga
     5761 tgaattttct acctatccag gaccaatcac tgctacgatc acttaccgtt taacaaaaaa
     5821 cgaattagag atgacaacct ctgctagtag ccttgttgaa accatctgca atccgaccaa
     5881 ccatgcctac ttcaaccttt caggaaatgg aacaagagac atctatgaac atcagttaac
     5941 ggtcttttta gatggtgtgc ttgaattaga tcaagaaaaa ctgcctactg gaaattggac
     6001 aaaaaaagaa aaactgccaa tcgactttag aaaatcccca acactgcaag agatccttgc
     6061 atgttatcca gatggactag atgatgtatt tcttttgcat catcctaggc tttccaaaac
     6121 ttccttacaa ttattcgaga agcattctgg tcgccaaatg actatagcaa cttctaataa
     6181 aagtatggta ttattttcta cgactggatt cgaagctgat ttctctgtca atggaaaaca
     6241 gatgcattca aactacggat tagcaatcga accgcaagaa atccctgata tcgttcattt
     6301 ccccaaattt ggatcgatca acctccatcc tggtcaagaa cggatcagtc agacgattta
     6361 tcggttctct gcacaataac aagagaaagg tgtttttatc atggctatta ccgtaaaaga
     6421 tgtggcgaaa aaagccggtg ttgccacctc gaccgtctct cgtgtcatca acgatcatcc
     6481 tagtatttcc gagacaacaa agaagaaagt ccgaaaaatt atggatgaac taggttatgt
     6541 tcctaacatg actgcccgca atctaggaaa acgcatttcc agtgccatcg gcgtaatctt
     6601 accccctctt gattcaaaag agcggatcgg taacccattt tatcttgaga tcatggaagc
     6661 aatcaacgaa gaagcgcgga attatgatat gactacagca atcgctactg ctaaaaattt
     6721 tgaattgctg ttagagaacg tccagcgtat gcatttacaa aaacaagtag atggttttat
     6781 tctagtgtat tcagatagtc aagatactgt cattgattat ttataccaaa accacatccc
     6841 tttcactttg atcggacagc cttatcatca tgagaacgaa atcgtttatg tagataatga
     6901 taaccagctt ctaggtaaac aagcaacaga atttttgatc cagaatggac ataagaatat
     6961 cctgtttgtg acaaacacga ctcatgaaag tttgtatttc gaacgatatt tcggttatca
     7021 aaaagcaatg atgatggctg gtcttcctgc gttatccagt gtagtactag agcatccgga
     7081 ggattatctg tcctttgaga aaacgttgaa agaaaacgaa gccacagcat tggtggtcat
     7141 tgacgatatc ttcgcccttc gcacgatgca gttggcgcaa atgtatggct atgtcattcc
     7201 agagacactc tccgtcatta gcttcaacaa ctcgatcttc tcaacattaa cacaccctta
     7261 ccttacaagt atcgatatcg atatctccga attaggacgt gtcgctatgc aaaagctgcg
     7321 cgaattgatc catgaagaat tgagcagtgg cgtacgtttg gtcatccctc atcgcttgat
     7381 caaaagagag acagtcgttc ctttagaaaa cccatactaa atagctgact cactaaatac
     7441 gctgaccatt tatcctatgg tcagcttttt atgtaaaaga agatactact tctaacacat
     7501 tgtttatctg t
//
LOCUS       DACECR010000120         7495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.121, whole genome shotgun sequence.
ACCESSION   DACECR010000120 DACECR010000000
VERSION     DACECR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.121"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            47..899
                     /locus_tag="IWO17_002519"
                     /pseudo
     CDS             47..899
                     /locus_tag="IWO17_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            996..2882
                     /locus_tag="IWO17_002520"
     CDS             996..2882
                     /locus_tag="IWO17_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0291374.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFETPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(2923..3825)
                     /locus_tag="IWO17_002521"
     CDS             complement(2923..3825)
                     /locus_tag="IWO17_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0291375.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            4055..5458
                     /locus_tag="IWO17_002522"
     CDS             4055..5458
                     /locus_tag="IWO17_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0291376.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            5458..6891
                     /gene="ascB"
                     /locus_tag="IWO17_002523"
     CDS             5458..6891
                     /gene="ascB"
                     /locus_tag="IWO17_002523"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0291377.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            7036..>7495
                     /locus_tag="IWO17_002524"
     CDS             7036..>7495
                     /locus_tag="IWO17_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0291378.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAV"
BASE COUNT         2396 a         1259 c         1694 g         2146 t
ORIGIN      
        1 tttttgtttt ggatacagcc ctatatatca tggaaaggag caaaacatgc tgattgagaa
       61 agtactgaat aacaacgtcg taataaccag ggatgaaaga aatcaggaaa tgatcgtgat
      121 gggaaaaggt ttggcattca aaaagaaagt gggtgacccg atttttgact catgcatcga
      181 caaaaaattt cagttgacaa atgacgggct ttcacaacag ttccaagaat tggcacaagc
      241 gattcctctt gagtacttag aaatttcttg tgaaatcatt gaatatacaa aagaggctct
      301 acaagtccag ttgaatgatt ctgtgtatat tgcattgacg gatcatttgt atacggctat
      361 cgaacgtcac aaaatgggaa tatttgtacc aaatgtattg ctgtatgata tcaaacaact
      421 atttcctaaa gagtatgcca tcgggaaaaa aaacagtgaa gaaaatctcg gataaatacc
      481 acatcaagtt atcagaagat gaagccggat ttatcgcatt gcatttagtc aatgcacaag
      541 gcattcaaag ccaacaagac aatacctacc tgttcactat aacaatacag gatattctaa
      601 atatcgtccg ctattactat aagattacat ttgatgaaga ttcagtccat ttttttcgat
      661 tcgtcacaca cttacgtttt tttctgacac gcgtcctaga tggagaaaaa tcagaagtgg
      721 gagaagtttc tgaggaattg tttcaggtta ttcaaaaaaa atataaggat acgtttggtt
      781 gcgttgagaa aatagcagac tttttagccg agacgttcaa ctaccagatg tccacggacg
      841 aaaaaatgta tttaacgatc catactgcgc gtttagtgca tgagaatcgt tgtaaataaa
      901 taaaaaaagg aggataaatt aacaagaacg tatgaaataa gggtagtgac attcagttgg
      961 ctaaaccttc cactaataaa ttggagggaa tagaaatggg aaaataccaa gaattagcga
     1021 gaaaaatcgt agaaaatgtc ggcgggaagg aaaatatcaa tggtttgaca cattgtatca
     1081 caaggcttcg tttcaaatta aaaaacgaag aaaaagcaaa tgatgagatc ttgaaaaata
     1141 tggacggtat tgtaacggta atgcgagcgg gagggcagta tcaagttgtc atcgggaatc
     1201 atgtgccagt cgtgtttgaa gaggttataa aagcaggaaa ccttactttt gatgaggctg
     1261 tttcaacaga aaaaatgaga ccgtttgata tgcttattga tggaatatcg ggctgtttcc
     1321 aaccattcct tgctatccta gctgctgacg ggatgattcg tggactgaca gcattccttg
     1381 tcttcttagg cgcgtttgac cgcggttctg gcacatttgt tatgtttgat aatatcggtg
     1441 attctgtatt ccaatttatg cctgttatta tcggtttaac agctgcccgt aaatttaaag
     1501 taaatgaatt cgtagggatg ttgcttggcg cagcattaat gaatcctagc ttgtcgctgg
     1561 aagctttgag tggtgcagca gaagcaccac taacgaccat attttctggc actatttttg
     1621 aaacacctat ttatcaaaca gtattcggca ttccctggat tgcgcgtaac tacgcttctt
     1681 ctgtcattcc cattatcttt attgtgttgt tagctagcca aattcagaaa ccaattaaaa
     1741 agttagtacc tgagatgatc gcgaatttct tcgttccatt cttcaccgtt cttattacaa
     1801 tgcctttagg ttttttactc gttggaccag tattcacatt cgcaactgat attttaatgg
     1861 ctggatttga aacactttta gcactttcgc cagttattta tggagcaatc gtaggcttct
     1921 tctggcaaat tctggtgatg tttggtctcc attgggcaat cgtaccaatg ggattgatgc
     1981 aattttctgt gaatggttgg caaaatatta tgacacctgt tgcagtagtg tcttttggac
     2041 aaactgcagc acttaccgca ctctacttta agcttagaaa tccaaaagac aaagcaattg
     2101 caataccagc cattgtttct ggtattgtgg ggattacaga gccagctatt tatgggttta
     2161 cattgccacg taaaaaaata tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt
     2221 atagtgggtt aatgaattta acttcttgga atcaaggtgg actaggcatc tttacaattc
     2281 ccaactatat tagaccagat ggtgatttaa cagatgtgat taatgtattg attgggatcg
     2341 ccattgcgat ggtggtatcc tttgcgctga cttttttctt ttggaaggag gaagcagatg
     2401 aaacagatga tagacagaaa agatcaggta aagaaattgt aaaaacaccg atacaaggac
     2461 aaatcgctcc actgaatgca gcaaaggatg cagcttttgc acaaggaaca ctaggtcgag
     2521 gaatcttgat ctaccctgaa aaaggagaag tacgtgcccc ttttgacgga acagtcatgt
     2581 ccctgtttcc gacgaaacat gcaatcggga tggtttctga agcaggtctg gaattgctga
     2641 ttcatgtcgg attggatact gttcagttgg aagggaaata ttttgaaagc ttggtgcagc
     2701 aaggagaaag agtgaaacaa ggcgatctgt tgcttcgctt cgacaaaaag aaaatcgaag
     2761 aggaaggata cagtttagaa acacctgtga ttgtcatcaa ttacatggat tatttggata
     2821 ttttggaaaa tcaaggggaa acggtcgggc cagacgatgc acttatcacc gcactgacct
     2881 gaacggttag gtgaaagaat ggactaggct taactattct ttttagtcca cttctttatt
     2941 ttcaaaattg aaaggctctt tcatgatggc tgagctgata gggcgtttat caaatttttg
     3001 tcctacagaa attagatgga ctaaattctg ttgataattt tctgcaaaaa cacaactata
     3061 taacacgtct agaagataac aaatcgatgt attgatcgta aaattaccaa ttttcgaata
     3121 cagtcgttcg cgtgtcgtaa gtcgcaaagc ggctgtactc attgaagata gcgttgaatc
     3181 accgatactg gtcaaagcta aaattggcac atgattttgt ttcaatattt ctgcggtacg
     3241 aaggatcaca ttgttttcac ccgtataaga aatcagaatc ccacacatat cacttgtgca
     3301 attgtacgct tcatagaacg attcaccaaa agtggtgaca atcgtgacat ccattttgat
     3361 gcgcttcatt ttcagcatga aatcttgaga aatcaaagta ttcgagttac tggcgaaaat
     3421 cacgattttt tttgcttgaa gcattagttg tttggctttt tgcagttggt catgatgaat
     3481 gagagaaagt gtatcttgaa tcgtcatctg ttctaaagaa gccattttat ttgcgatcgt
     3541 cataataccg tctgtggtgg aaaaaggaag attcgcatct acgtcttgaa aattcccttc
     3601 aagataggct gtttcagcta aaaaagcatt acgaaaactg acccagccat caaaacccat
     3661 tttttttgct acacggatga gtgtggacgg gtggacataa catcgctcag caatctcttg
     3721 gatggtcatt tcgccaagtg ctggagaatc gcttaagaga aactgaatca cttcattttc
     3781 tgaaggagaa aaggagattt ctttgatttt ttcacgtaat aacatgtaga taccttcttc
     3841 ttgttttttt acgtaaatta taactttttt gaaaatgatt tgtaaacgga tttatgttat
     3901 atagcttaat aaagacattt tttgacaaga tatttacaaa aatccagaag aaaacactaa
     3961 cttttgtaaa tgttactgga aagaaacgaa tttctattgt ttgttttgtt tacaaaaaag
     4021 ataataaaag tgtaaaaagt aaaggagtga gaacatggca aaaggaatca aaatcgctac
     4081 gattggtgga ggcagcagtt acacccccga gttgatggaa ggatttatca aaagatatga
     4141 ggaattaccg atacgagaaa tctggctcgt agatgtagaa gaaggaaaag aaaagcttga
     4201 gatcgtcagt cagatgtctc agcgtatgtg ggatgcttct ccgtatgaag tcaaaatcta
     4261 tgcaactctg gatcgggaag aagcattgaa agatgcggat tttgttacga ctcagtttcg
     4321 tgtcggtcaa ttagatgcac gaatcaaaga cgagcggatc cctagttatt atgggatgat
     4381 tgggcaagaa acaaatggtg ctggcgggat attcaaagct tttcgtaccg ttccagtggt
     4441 tttggcaatc gttgaagaca tgaagcgttt atgtccaaat gcttggctga ttaatttcgc
     4501 gaacccaagc gggatggtga cagaagcagt tatcagatat ggcaaatggg ataaagtgat
     4561 cggattatgc aatgtgccag taatggcgat gatgacagaa ccaacaatgc ttggcaagaa
     4621 aaaagaagag ctgatctaca agtttgccgg tctaaatcac ttccattggc acaaagtagc
     4681 agataataaa ggaaatgacc gcacggcaga attgattgat cgtttatacg atgaagacaa
     4741 tggattgccg aaaaatatct ttgacatccc gtttttcaaa gaacaattaa aacaaatgaa
     4801 catgatccct tgtggctacc accgctatta ctaccgtgaa gaagaaatgc tggaacatgc
     4861 tttagaagaa tatcaaacga ttggtacaag agcgcagcaa gtgaaagaaa cagaagcaga
     4921 gctatttgaa ttatacaaag atccagaatt agaccataaa cctgaacaat tgcaaaaacg
     4981 aggtggcgct tattattcag acgctgcctg tgaaacgatt gcttcaatct atggaaataa
     5041 agaaacacag atcgtggtat ctacaaagaa tgatggagct gttcctgacc tgccggcaga
     5101 ttgtgtagta gaagtcactg cctatgttgg cgcacaagga gcgagaaatg tagcgtttgg
     5161 ttctcttcct gctgctgaaa gaggctggtt gcaagtaatg aagaatatgg aattgctgac
     5221 gatcgaagca gctgtgactg gtgattatgg gacagcttta caggcgttta cgatcaatcc
     5281 gcttatacga tcaggaaaga cagcaaaacg gatcatggat gagttgttta ttgcacacca
     5341 gcagtattta ccgcaattca aggaaacaat cagccggtta aaagcagaag gaatcgaagt
     5401 ccaagatgaa ctggcaagag aattatcaag caatacacat gtaatggagg aagtatgatg
     5461 ggattcagaa aagatttttt atggggcgga gctacagcag ccaatcaatg cgagggcggt
     5521 tatgatgaag gcggacgtgg gttagcgaac gtagacgttg tgccaatcgg agaagaccgt
     5581 tttcctgtca ttactggaaa aaagaaaatg tttgaatttg atgaaaatca tttttatcca
     5641 gcaaaaattg cgatcgatat gtatcatcat tataaggaag atattgcttt atttgcagaa
     5701 atggggttca agacttatcg gatgtccatt gcttggagtc gtatttttcc aatgggcgat
     5761 gaaaaagagc caaatgaatc tgggttgaaa ttttatgagg atgtctttaa agaatgtcaa
     5821 aagtacggga tcgagccgct tgtgacgatt acacattttg attgtccgat gcatttgatc
     5881 aaagaatatg gtgcgtggcg ttccagagaa ctcgttggtt tttatgaaaa tctgtgccga
     5941 gtcatcttca atcgctataa aggacttgtc aaatattggt tgacattcaa tgagatcaat
     6001 atgatccttc atgcaccatt catgggcgca gggctgtatt ttgaagaagg agaaaatgaa
     6061 gaacaaatca aatatcaagc ggcacatcat gagttggttg caagtgccat cgcaacgaag
     6121 attgctcatg aagtagatcc ggagaacaaa gtaggctgta tgctggcagc aggagcatat
     6181 tatccataca cttgcaatcc tgaagatgtt tgggcttcta gaaaatcaga tcgagaaaat
     6241 tatttcttca ttgatgtcca gtcacgagga gaatatcccg cttatgcatt gaaagaaatg
     6301 gaacgaaatg gcattcagat cgaaatggaa cctggggatg ccgcattgct gaaagaacat
     6361 actgttgatt ttatctcctt ttcttattat tcttctcgag tttcgactac tgacaacgaa
     6421 ctcttggaac agactgcagg aaatatcttt gcttctgtca aaaatccata tctggaagca
     6481 agtgaatggg gttggcaaat tgatccatta ggcttgagaa taacgatgaa tgatatttat
     6541 gatcgttatc aaaaaccact atttattgta gaaaacggct taggtgcagt cgatacgccc
     6601 gatgagaatg gttatgtgga agatgattat cgaatcgact atctggcagc acatatccaa
     6661 gcaatgaaag atgcagtaga attagatgga gtcgatctat taggctatac gacttgggga
     6721 tgtattgatt tagtatcagc tggtacaggt gagatgaaga aaaggtacgg attcatttat
     6781 gtcgatcgtg acaacgaggg aaatggcaca ttagcccgtt cgaagaagaa atcattcaac
     6841 tggtataaaa aagtaattga ttcaaatgga gaaaatcttt cgaatgattg atcaatgatt
     6901 tataaaagtc tattgttttt cagcggattt ttcgagtata ataaagctat tcgagaaacg
     6961 caagcagaac aatagacttt ttttcgcatt ggctgtaaag tatgcttact tgattaagta
     7021 aaggagaatc tacatatgta tgatgtaatc gttattggcg caggtcctgc tgggatgact
     7081 gctgctttat atgcttcacg ttcaaactta tccgtattga tgatcgaaca aggcgcccct
     7141 ggcggccaaa tgaacaatac ggcagaaatc gaaaactatc ctggtttcga ttcaatcatg
     7201 ggtcctgatc tagcttataa gatgtatgaa ggtgtttcaa gatttgggac agaaaatgct
     7261 tatggaatcg tgcaagatat caaggatcac ggcagccaca aagaagttat ttgtgaagat
     7321 aaaacttacg aaggaaaaac agtcattatc gcaacaggct gtatccaccg taaattaggc
     7381 gtacctggcg aggaagaatt tgctggacgt ggcgtttctt actgtgcagt ttgtgatggt
     7441 gcgtttttcc gtaacaaacg tctagtagtg atcggaggag gagactcagc agtag
//
LOCUS       DACECR010000121         7454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.122, whole genome shotgun sequence.
ACCESSION   DACECR010000121 DACECR010000000
VERSION     DACECR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.122"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            273..1751
                     /locus_tag="IWO17_002525"
     CDS             273..1751
                     /locus_tag="IWO17_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0291379.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2028..4088
                     /locus_tag="IWO17_002526"
     CDS             2028..4088
                     /locus_tag="IWO17_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291380.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4171..4740
                     /locus_tag="IWO17_002527"
     CDS             4171..4740
                     /locus_tag="IWO17_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0291381.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4915..5721)
                     /locus_tag="IWO17_002528"
     CDS             complement(4915..5721)
                     /locus_tag="IWO17_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291382.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5859..7373
                     /locus_tag="IWO17_002529"
     CDS             5859..7373
                     /locus_tag="IWO17_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ0291383.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         2674 a         1227 c         1452 g         2101 t
ORIGIN      
        1 aaagagcagg acggtggccg tcctgctctt ttttgtggtt gtttacgcaa atggcgcaaa
       61 tcttgtacat tttaacacaa taaaacaaaa aatatagaat atttcctcat aaatttttcc
      121 attttttgca atattctcaa aaaaaagata aaaaatgaga ataactgatg aaaaaacaag
      181 cataatccat cataaaatca gtataggtga aaagatgaac tttcatctgc atatcaagta
      241 attggtatga ggcatttttt gaggtgtagg atatgaagtt ggattacctt atagaagagg
      301 aagatagaga aaaactgctg ctagctagat atttagaatt tgagaaaaag aattatgtag
      361 tgctgcctaa gttatatgaa acattaggtt tgtctaaatt taaaacaaga aattatatca
      421 tagaattaaa taaagaattt actgaattca aaacaaagcc agagatccaa atttcagaag
      481 aagcagagat ccatgtcgaa cgtctgaatt tgacggatat caaaaattta agaagaatct
      541 actttcaacg atcaaaaatt gctcgactgt taacggagat cgtggattca aaagtaacga
      601 tcgaaaaatt tgcagagaat cattttttaa gcatatcaag agcctatgta aaaagaagag
      661 aattagtctc ttttcttgaa cagcatcaaa taaagttaaa gaaaaacata ttagttgggg
      721 aagaaataaa catacggaac gtattatttg gggtatattt cggggtcttt aatggttttt
      781 ctatgccatt tagagaaagc gtcatgaatg aagcaaagag tatcgtgcaa tattttgaat
      841 atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa attcaatttg ctaaccacta
      901 ttagtttgtt gagggcctat gggaatcaac caatcggaaa acgtttcttt tcagatgaat
      961 actttaagag ctcagaagga atgaagatca tcaaggattt atcacatatt tcttttcttc
     1021 atatccctaa agagcagatc attgatgaga tttcttatat catgttgttt ttgatcacgg
     1081 aagaagaatt tgaattagat ggcgcatttg gacttgcgga agctcatttt catgatatcc
     1141 acatgattag tcaaaaaatg tcagtggaat tgattgatca attaccattt tcgattgaaa
     1201 tctctgaaga agaaagacta tatatacgaa aagaacttcg tatgggtatg atgcatgtca
     1261 atcgaaaaaa ttggatattc gactttgaga tgactagctt tacgtctaaa aagcagctac
     1321 agtttttctt cgagacgtat ccaatattca gtacagcaat ccaaaaggtc atacaagaga
     1381 ataaacgcta tttcttggga aaagatgaac gtttcatgat tcgcttattt tatgattatc
     1441 tctttttatt ggtcggtatt tgtccggtat catattttga agcaccgatt catgtctgta
     1501 ttgatttttc gcaaggatct gcttataccg attacattat ttctcaagtg aaaggattta
     1561 aaaattacaa tattgtgatt gagagtcgaa tcacttctcg tacgaatatt tatttatctg
     1621 attgtttact tgaaaaattt aataaagaac agataatttg gaaaaatcca ccgactccag
     1681 atgactggga atattttgga aataccatca tcagagaaaa aaatagacat ttaactgaaa
     1741 aaatcagcta gtatttatgg atggcagctt tgtctcacat atttattagc tgatttatgt
     1801 ttttttcaaa gaaaaggtaa ctagatataa ctagatataa ctagatatat tacttaacaa
     1861 aattaaaaag tacataagtt attattccga ttttcttgaa acaataaaaa atcaaaaaaa
     1921 atactctcct aatagtccat cttcttttga ttttatttat ataatgaaat catcttatga
     1981 aaattactac ttcatgttta gacaagagta gggaggacta agaataaatg tgttcaaaaa
     2041 gacgttcaaa acaactaaat aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg
     2101 aaaaacacta cgttaagttt atgatgcgca cgatgctgat cagtttatct gtaggtgtct
     2161 atatgcatgt acccgttcag gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt
     2221 ctgagaccac tgattcccct atcactatta ctcatacttt cactgatgca aatggaaaac
     2281 agctacttcc tgcattgact tcctataata aagtcagtta tcctccaacg ataaaccact
     2341 actggttaat tcctaacaaa acattatgga catcggaaac cggaagcagt gtgccgcttt
     2401 ctatcttttc cgatatgact gatcctacaa aaggtgatca gttaaaacag atcactgata
     2461 ttttgaatca tgctttgaca gatataaagg acggtggaag cgcacaagtt tcttatgtat
     2521 atgaaaaaga ttattcacag tttgaaagaa agaaaatcga tgttctggca gggacaaaag
     2581 tttcgatatc tgatctggta gaaaggctga agaacgcaga tggaacacaa ggagatatgt
     2641 ccaaagtaat ttcaaacaat gggaatatcg tggatacgac taatacagga acatctacta
     2701 tttccttgtc ctattttgat ccaattgctt taaaaacaat gactgcatct gctgtgatcc
     2761 atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct tcacttaaga acaggagaga
     2821 gttacgcccc taaagatctt gttgaatatg taacaaaaag cgatggaact tcaggaaata
     2881 aagaagaaat caaaatgaac gggacagtag atactacaac tccgggagaa tatcccgttg
     2941 tattaactta tatagactcg aaaacatcaa atgtggcaga aacaacagct gtagtttatg
     3001 tagaagcatc tgctgcttca tcgactgtca tcactcattt tatagatagt caaacaaata
     3061 aagaacttca agcagaaaca catatgagta gtgtaaatcg tattcctgat ttgcgcctac
     3121 ctgctgaatt ttctgatcga gtgccaaata tagatttatc gacttatgaa caaaatggga
     3181 aagtcacttc tatttctgat tggatgcaag caaacggatt agacagaaaa agtcactggt
     3241 cggagatcct tgaagtactt cactcagaac tgatgaataa taggaagcaa tcaggagggg
     3301 aaattacctt tacttatgtt tatataccag acaatcgttc attagaaggt catgatctta
     3361 ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat ccaatcagca acaaattcct
     3421 taggtaaacc cgtaccgtta aaggatgtat cggttacaat caatgaagta ccattggagg
     3481 atggttattt gaccaaacaa acaggtgatt ttacgattat gtatagttat catgacccgt
     3541 attcactcca agaactccat gctcagtcac tattacatgt tttaccatta aacaaacaga
     3601 tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc agaaaaaaaa caacaaatgg
     3661 cccatgcagt ttctccaaca gggaaagtaa aacaaacaag aagcctagag ttagcaagaa
     3721 tatctagaaa aatctcagaa tctaataaaa cgataaggca agaagctcat ttggccccac
     3781 aagtttctgg attggaaaat gaaattttag agcctaaaat aaaacaacaa tcagaaaaag
     3841 aatttgttgc taaacagtcg gaagatcaga aggaatcaga tgaccagaag gaaacgaaga
     3901 aaaaacgctt gtcaactatt gattccaccg atgagcatct gcagccgcaa gtaccacttc
     3961 ctagtccggg aacacccgca cctggtggag gtattccttc ttctgcttta ggagatttca
     4021 tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa tgaatttgac gcagatgact
     4081 ggttctgatt tatatctcta tctgttatag tcaaataagt agctgagtca tatgttattt
     4141 ggcataatgg agacgaggag ggatagaaaa atgttagata aaacgttacc ttacgcagaa
     4201 atctggatga caagaccact tcaacaaaat gttccaatca cgcctttagc ggaaggtttt
     4261 gaatttcgat tttatcaaaa agaagatgaa tctgcttggg cggagattga gacttccgtt
     4321 ggggaatttc aaacgacaga tgaagcactt gagtattttc aacaaaactt tgctccttat
     4381 cctgaagaac ttgaaagaag gatgtttttt gtagagacaa aaaaaggaga aaaagtagca
     4441 acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt atccggtttt tcattggtta
     4501 gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg
     4561 caagaatttc ctaccctcca tacggacgga ccaatctatc ttcacacaca gacatggagc
     4621 catcaggcga ttgctcttta taaaaaaatg ggttttactt ttattgctga aaatctagac
     4681 ggcacaccta atccagatta tgaaaaagtt atgcaggttt taagtaaatt agataaataa
     4741 cgaaatggtg aaataagtcc tcagtattgg gataaaaaac gaaatagtca ttcctcatat
     4801 cttacttttt tgagttaata ctatcggata actaatggca tgccatacat tcaaaaaaga
     4861 aggggcagtg acaaaagtat tgtcactgcc ccttctcttg ttttttagca tgttttatcg
     4921 tttgattttt ttaggaataa aattcttgat gaacgtaaat ttttggtaat cgtttcgact
     4981 atagaggtaa ccaaatacac tgaaaacaac tgctccaata aaattaacaa ataaatcctt
     5041 catagtatcc atgattccca cgtctaagta accattttgg atgattgttt tatctgtttt
     5101 accatctgca ttttgacttt caatgattgt ccgagtgata tctggaacat gatagacagt
     5161 gtttccgtct ttacttaact ccacactatt gatttgctgg atgatcgtat ctttttgcat
     5221 atcgaattgc agtaatttat ctgctgaaaa ttccacaaac tcccacataa caccaactgt
     5281 catcgaaaaa cagaaggaaa caactgctag taataaagga gtcaagttca tggtttttgt
     5341 atttttattg agcaaataaa caagggaaaa gccgacaccc ccagctaaaa atccgctgaa
     5401 tgcatgtaga aaagtatccc acattgaaaa atagccataa aaatcactta actctcctaa
     5461 aactgttgaa ctaaaaataa aagataacaa aatgatttct agcatatttg gaatctgtat
     5521 ctttaatatc ttttccagaa aataagggat ttcaaataaa attaatgtca gtaataaaag
     5581 cattgtattt ccccagtgtt ggttccagac ctgatccact agcataataa cgacgatgat
     5641 acgtaataaa atatagattc cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa
     5701 aaaggttttc aaatgattca taaaataact ccttcatgta tatccttaat tatactaaga
     5761 agcgtgtgtt taccaaagct atatgactta aagaaaaaca gagttgatat tgtgaaaaaa
     5821 atcacttaat atagtataag gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt
     5881 tattaacagc cgttgcactt cttgctatga gcgcatgcgg cgcagatcag aaaaatacag
     5941 cgcagacaag ttctagtacg aaggaatctg ccgaagtatc atcaggcgct tctgaacaag
     6001 tatatacaga tccaaaagaa atgaaagaat cttatgatgt cgtgattatt ggagctggtg
     6061 gtgcagggat ggcagcagct atcgaagcaa aagatgctgg gttgaatcca gtaattttgg
     6121 aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc aagcggtatg aatgcttcag
     6181 aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa tgatgccttc tatgaagaaa
     6241 cgctaaaagg tggtggagga acgaatgata aagaaatgct tcgatacttt gtcgatcact
     6301 ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa actggacaac ttaacgatca
     6361 ctggagggat gagtgaaaag aggacacatc gtccgtctga tggctcggct attggtggat
     6421 acttagtgga ggggttattg aataacgtcc atgagcggga aatccctatt tttgtcaacg
     6481 cagatgtgac agacattaag aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc
     6541 aaggagaaga accaaaagag gtcaccggaa aagcaatcgt agttaccaca ggcggttttg
     6601 gtgccagcaa agaatttatt gaaaaatatc gtcccgattt agctgattat attacgacaa
     6661 atcaagaagg atcaactgga gatggtatca ccatgattga aaaattaggt ggacaaaccg
     6721 ttgatatgga taaaatacaa attcatccaa ctgtgcagca ggatgagggg gtcttgattg
     6781 gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca agaggggaaa agatttgtca
     6841 atgaaatgga tacaagggat aaagtatctg cagcaattac agcattgcct gaaaaatcag
     6901 cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc
     6961 aaaaaggata tgtcacaact ggaaagacaa tggaagaatt agcagaaaaa atcgatttac
     7021 caaaagaaac actagcaaaa acagttgatg gatggaatac ggatgttgcg gagaaaaaag
     7081 atgctcaatt caaccgtgcc acagccatgg aacatgactt atcgtctccg aattattatg
     7141 cgatcaaaat cgcaccagga atccattaca cgatgggcgg tgtgaaaatc aatacgaaca
     7201 ctgaagtatt agataaagaa aaccagccaa ttagggggtt atatgctgct ggtgaagtca
     7261 caggtggctt acatggaaac aaccggatcg gtggaaattc agttgcggag atcattattt
     7321 ttgggcgaca agcgggcata caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa
     7381 cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta ataccgaggg tcagctgatt
     7441 gaacactgtt tagt
//
LOCUS       DACECR010000122         7368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.123, whole genome shotgun sequence.
ACCESSION   DACECR010000122 DACECR010000000
VERSION     DACECR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.123"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1152)
                     /locus_tag="IWO17_002530"
     CDS             complement(<1..1152)
                     /locus_tag="IWO17_002530"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ0291384.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1337..3778
                     /locus_tag="IWO17_002531"
     CDS             1337..3778
                     /locus_tag="IWO17_002531"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ0291385.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(4108..4812)
                     /locus_tag="IWO17_002532"
     CDS             complement(4108..4812)
                     /locus_tag="IWO17_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291386.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(4999..5244)
                     /locus_tag="IWO17_002533"
     CDS             complement(4999..5244)
                     /locus_tag="IWO17_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291387.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(5261..6112)
                     /locus_tag="IWO17_002534"
     CDS             complement(5261..6112)
                     /locus_tag="IWO17_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0291388.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            6278..7282
                     /locus_tag="IWO17_002535"
     CDS             6278..7282
                     /locus_tag="IWO17_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ0291389.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         2320 a         1324 c         1523 g         2201 t
ORIGIN      
        1 tattttgtag taacgaatat cattttcgat ctttgtaatc gaaaaaggta catctgaagt
       61 atattctgca cattctccgt gttcaggaag cgtctcactg aaaatcgttt catattcgtc
      121 aacagtgatc cgtgttcgag aatctagcat ctcttgatga gattgtgcga agagatattc
      181 ttgatagttt tcttccagat aacctgtaaa aaattcactt actgcaccag aaccgtagct
      241 gaatagaccg atgcgatctc ctggctgtag tgatttcgaa ttttccagta gagaagttag
      301 ccccaggtat aatgaaccag tgtaaagatt accgattcgt cggctgtaac ggatgctttc
      361 ttcatagcga gccataagac gttcctgatt ttcttcgtct gtttggtcta atacgctttg
      421 caatgccttt tttcccatct tagtatacgg aatatggaaa gcaatcgcct gataatcttc
      481 gagtcctcga cccgacaatt ctttatgtcg attccaaact ttttggaatg attcgatata
      541 cgtagaatta gataaaggac catcaacaac aggaaattcg ctataatctg gacgccagaa
      601 atcatagata tcttctgtca gaaatacgct gtcgtcttca atcgataaaa tacgcgggtt
      661 ttgagtgatc atcatcgcaa cagcaccgac accttgcgtc acttcaccac cgcttgccaa
      721 gccgtaacga gcaatatcac ttgctatgac tagtactttt cgttctggat gatttttgac
      781 atattctttc gccatgtgca aagctgcggt gccaccgtag caagcctctt ttacttcaaa
      841 agaacgagca aaagattgga ttttcagtaa atggtggata attaccgcac tcgcttttga
      901 atgatcaatc ccggattctg ttccgacgat gaccatgtcg attagctcac ggtctttttc
      961 tgttacgatt ttgctggcag catttgctcc tagtgtaatg atgtcttcat ttgcacgatt
     1021 gactgccatt tggtcttgac caatccctat atggtatttt gcaggatcat ccccacggct
     1081 ttctgccaac tctgtcatat ctaaatataa attaggaata aaaaaggaaa gacgatcaat
     1141 ccctattttc atttcaacac ctctttactt atgataaaaa acttacttta ctaaggtatc
     1201 ataaaaagat taaaagaaat ataaaaaaga gaatatttaa ggattgttaa aatttaatct
     1261 tagaaaaaaa taactggaac aagagctatt tgtttttaca gcaaagaatg gtatattttt
     1321 gtaggaggtg gcttacttga aagaagtcgt tatgatagat gctgcaagaa cgccgatagg
     1381 aaagtatcgc ggaagtttaa gtccatttac tgcagttgaa ttaggcacac ttgtgactaa
     1441 aggattatta gataaaacaa aactgaaaaa agataaaatc gatcaagtaa tatttggaaa
     1501 tgtcctgcaa gctggtaatg gtcaaaatgt tgccagacag attgctttga acagcggtct
     1561 ccctgtagat gtcccagcaa tgacgatcaa tgaagtttgc ggatctggta tgaaagcagt
     1621 gatcttagca cgccagctga tccaattagg tgaagcagaa cttgtgatag ccggcggaac
     1681 agagagtatg tctcaagcac ctatgctgaa acagtatcag tcagaaacaa atgaatatgg
     1741 tgaaccaatt tccagtatgg tcaacgacgg attgactgac gcattttcaa atgcacatat
     1801 gggattaacc gcagagaatg ttgcaacaca attttctgtg agcagagaag agcaggatcg
     1861 ctatgccttg tcgtcccagt tgaaagcagc acatgctgtc gaagccggtg tattttctga
     1921 ggagatcatc ccagtcaaga tttctgatga agacgtgtta tctgaggatg aagcagttcg
     1981 tggaaatagt acattggaaa aactgggcac gttacgtaca gtattctcag aagaaggaac
     2041 tgtaacagca ggaaatgctt ccccgttgaa tgacggtgcc tctgtggtga tccttgcatc
     2101 caaagaatac gcagaaaata ataatctgcc ttatttagca accatcaaag aagtagcgga
     2161 ggtcggtatt gacccaagta tcatgggaat cgctccaatc aaagccattc aaaaattgac
     2221 agatagatca ggaatgaatt tatcaacaat cgatcttttt gagatcaatg aagcatttgc
     2281 agcttcttct atcgtagtta gccaagaact gcagttagat gaagaaaagg tcaatatata
     2341 tggaggagca atcgcattag gccatccaat tggtgcaagc ggtgcacgta tcttgactac
     2401 attagcttac ggattgcttc gtgaacagaa gagatacggt attgcttcat tatgtattgg
     2461 tggaggttta ggcttggcag tcttgttaga agcgaatatg gagcaaacac ataaggatgt
     2521 gcaaaaaaaa aagttttatc agcttacgcc atctgaacgg cgcagtcaat taatagaaaa
     2581 aaatgtattg acacaagaaa ctgcgttgat ttttcaggaa caaacacttt ctgaagagct
     2641 ctccgatcac atgatcgaaa accaagtgag tgaagtggaa atcccaatgg ggatcgcgca
     2701 gaattttcaa atcaatggca agaaaaaatg gattccgatg gcaacagagg aaccatctgt
     2761 cattgcagct gcaagtaatg gtgcaaaaat ctgtggaaat atctgcgcag aaacacctca
     2821 gcggctgatg cgagggcaaa tcgttctatc aggaaaatcg gagtatcaag cagtgatcaa
     2881 tgcagttaat catcgaaaag aagagctgat tctttgtgca aatgaaagtt atccttcaat
     2941 cgtgaaacgc gggggcggtg ttcaagatat ctctactcgg gaattcatgg ggagtttcca
     3001 tgcatactta tctattgatt ttttagtgga tgtaaaagat gcaatgggcg ctaacatgat
     3061 caactcgatt cttgagagtg tggcaaacaa gttacgggag tggttccctg aagaagagat
     3121 tctattcagt attttaagta attttgctac agaatcattg gcttctgcct gttgtgaaat
     3181 cccattcgaa cgcttaggga gaaacaaaga aatcggtgaa caaattgcca aaaaaatcca
     3241 acaggcaggg gaatatgcga agcttgaccc ttaccgtgca gcaacgcaca acaaagggat
     3301 catgaatggt atagaagctg tagtggcagc tacaggaaat gatacacgtg cggttagcgc
     3361 ttcgatccac gcctacgcgg caagaaatgg attataccaa ggattaacag actggcagat
     3421 caaaggggat aaactagttg gaaaactaac ggtgcctttg gctgttgcca cagtaggcgg
     3481 agcttccaat atcctgccaa aagcaaaagc atctttagct atgttagata ttgattcagc
     3541 caaggaacta gcacaagtaa ttgctgcagt agggttagct caaaacctgg ctgctttgcg
     3601 tgcattagtt acagaaggaa tccaaaaagg ccacatggga cttcaagctc gttcattagc
     3661 gatctcaatt ggggctattg gtgaggaaat cgagcaagtt gcgaaaaaat tacgtgaagc
     3721 agaaaagatg aaccagcaga cagctataca aatattggaa aaaatccggg aaaaataaaa
     3781 aagcaatatc tttagaagaa taaagcaaga tttacagtta ttttatttac tgcagatctt
     3841 gcttttttct ctatcagaaa atatgtttat tattagaatt ttttatagtt aaggttaggc
     3901 atcttaagaa atcaggcaac tccaagatat cttcaggtag aagagaagtc gcctaacatt
     3961 gtaggatcat aggtgatttg ttggacaggt caagctgata tttgagcctg atgttagaag
     4021 gaatttcttt ttacttgctg tagaaattat tatatgacta aaaaggctgg gattttaaat
     4081 cccagccttt tatgacaaat agaattttta tcgatcagta atcgctgtcg ctgcaaattg
     4141 ttcgatcaaa ggaatcaaag tcaatttgac tttgtatttc cgttcgagtg ctttttcgat
     4201 aacgaactca ttttcagtag tcatagaaat agttgctaat ttacgaggtt catcagcatt
     4261 tttttcatta ataactactt catgttcatt ttgttctgtg taaaatttag gatactcagc
     4321 aacggctttt gaaaaatcag caaaacttaa agcggcattc gtatcagttg agcgtgtatt
     4381 ttcagttgtg tccgcttttg gtttttccgt tttttcttct ggcacactga ctgtttcttc
     4441 tgtaactggt gtcgcaacag cttgcgcagt tggttcatcg tttttcaatc gttgatcatt
     4501 ccagtaagac acgatttcaa taagaagatt gtcacggaat cgttcgaaaa tcccgttttc
     4561 tcctgaacga attctcttta atacagtatt gaagttgtca tcgattggca cgttgatccg
     4621 tgtatcttca tttgttttcc cagctaatcc gatcgttaag atttgacggt ttttgggatc
     4681 aagcatgact tgtaacggca aaccataacg tcgcaagctc tcttcttcta gagaacggaa
     4741 taattgtcct cttacaccat ccaatgaagg acggaattga tttaataatt tttgtttctg
     4801 ttttttcttc atatgttctc tccttcgctc ataccctttc gattataaga aaaaaacagc
     4861 caaatgaaaa ggagtaatgc aaaaaaactt gcgtatacgc aaattacaac agaaaaattt
     4921 gcgtattttc ccgttttttg aaagaaaaat aaaaaatgga acggattatg tatatataca
     4981 taatccgttc cattctttct attttttcgg tatcaaggca aaaacaggta gttcaggtga
     5041 ataaatgact tcatgttcgt gccagggttt gaccattagc gaatgatcga accgtttttt
     5101 cagttcttcg atgatttgat catgtgtcca gtttcttgca ggaaaatgtt ggattttctc
     5161 tgcatcgatc aaaaagacaa aatcggaatg aaaagtctgg gtaacttctt ccgagagaga
     5221 attcaagcgt tcgattggat ccataaaatc acctttcgac ttaaaattgg gtagtatctg
     5281 ggtcttttgc tttgccgact acaccatcta actgatcgat ttgtttcatt tcttcttctg
     5341 ttaattcaaa atcaaataat tcagtatttt ctttgattct gcttggtgtc actgacttag
     5401 gaagaggaag gaattcgtgt tgcagcgacc aacgcaaagc aacttgtgca atcgtcttgt
     5461 catgtgcttc agcgatttgt ttcatttcag gcacatcgaa gattttacca gtgcctaatg
     5521 gactatatgc ttcgaggatc atatcatgtt tttttgcata ctcaactaca gttgtctgta
     5581 attcgcctgg tgcaaggaaa acttgattga ccatcggcat aatttttgct gtttttgcta
     5641 gtgtatctag atggtggggt aagaaattgc tcacaccgat tgctttgatt ttacctgctt
     5701 catacagttc ttcaaatgct cgccatgtat ctgcatttgc ttgttcccaa ttatcacgaa
     5761 aagcaacagg gtttggccag tggatcaaaa acagatctaa ataatctgtt tggagcttct
     5821 tcaatgattc ttcaaaagaa gacatcacaa gttcatagct gtggtttgca ttccataatt
     5881 ttgttgtcaa gaatatctct tctctaggaa ttccactttc cttaatcgct tggccgacgc
     5941 tttcttcatt cttgtatcct tgggcagtat cgatatggcg ataacctgca gccagcgctt
     6001 ctttaacaga ggaaacagcg acatccccat caggtgtttg ccatgtgccg aatcctacta
     6061 ccgggatttc gtaaccattt gatagacgat aagtgcttgt taatgaattc atactttcag
     6121 ctcctttttt attcgttcaa ctataagtgt agcgcatttt tcgaaaaaaa gaatccattt
     6181 tgtttctact cttgaaattt ttgtttaaaa gaaaatgatt ttgattctgg tatttttgat
     6241 tttttttccg taaactaaga cagaagggaa tgagaaaatg gatatctatt taaaaaaagc
     6301 aattctgcat attattgaca gagaaactgg ttctccagtc ttttcacaaa aagagctgga
     6361 tctgacaaaa gaatatattc gtgattttct tcaaaagaag atccaaaaaa tctcttctgc
     6421 tcaaacaaaa actggacaac tagcagaaga aagcatgatc gttgaatcac ttaaaatcgc
     6481 tcaagaaaat tttgtggaag caagtgaagc aatcgttcag cggtggttcg atatctacca
     6541 agaaagtgaa gaagcaccta gtgcagatgt atttgttgtc ttgtatgaat tagatacagt
     6601 gatgtactta tctttattga aagtcaatta tcgtgaggct tacactcact ttgttgaagc
     6661 agacgaagca gggatcgaca accgcttgat tttgaatcga gcaattctag gggcgaaaac
     6721 acaaaaagca gatgaagcat tcgctgtgaa tttgactgat tggacctacg aattaattga
     6781 aaaaaaatat gagttttctg gcaaaaaaga gctttatttt tctggaagag tgatcgaaag
     6841 tgttcctgca ccttctttag aagaaaatgt aaaagtgatc caaaaagtag ccaagcatct
     6901 tggaaaaaaa tttgagacag aagaatttga tatcatggct gatttgaaag aagctgtcta
     6961 tgatacgata gaagaaaaag gtcggttgga tcatgagatg attgcggaaa aagtcttcaa
     7021 ggaaaacatc acggcaaaaa tggcttttaa agaagaagta caagagcaag gatttgtccc
     7081 agaagcacct cctgtaaaag aagtacaaga aatttctgag aaaaaatttg gaaaacaaaa
     7141 attcaagcta tcaaacggga tcgaactgat cgtaccagta gatgtatatc gcaacccaga
     7201 tttgattgaa tttgtgaata atccggatgg tacgatatcc gtgatgatca aaaatgtgga
     7261 tgaagtattg aatcgtttat aaaaatgact agaatattag gtgttcaagt accagtgatc
     7321 agttaaatga attgtgtcaa gagaaggatc ggaaaagacg aatataac
//
LOCUS       DACECR010000123         7314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.124, whole genome shotgun sequence.
ACCESSION   DACECR010000123 DACECR010000000
VERSION     DACECR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.124"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            234..554
                     /locus_tag="IWO17_002536"
     CDS             234..554
                     /locus_tag="IWO17_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291390.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHILHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            1006..1206
                     /locus_tag="IWO17_002537"
     CDS             1006..1206
                     /locus_tag="IWO17_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0291391.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1353..4142
                     /locus_tag="IWO17_002538"
     CDS             1353..4142
                     /locus_tag="IWO17_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0291392.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKATLKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4189..4716
                     /locus_tag="IWO17_002539"
     CDS             4189..4716
                     /locus_tag="IWO17_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0291393.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGYESGDEVKTIKGI"
     gene            4737..5927
                     /locus_tag="IWO17_002540"
     CDS             4737..5927
                     /locus_tag="IWO17_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0291394.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5967..7265
                     /gene="asnS"
                     /locus_tag="IWO17_002541"
     CDS             5967..7265
                     /gene="asnS"
                     /locus_tag="IWO17_002541"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ0291395.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
BASE COUNT         2446 a         1338 c         1557 g         1973 t
ORIGIN      
        1 tcaagaaata agaagagatt gacaaaaatt atttctcaaa tttttgtgaa atttcggctt
       61 atttccgaag aagctacttc tggaacaccg tttaaaaaag aggttgtgac gtggtcacga
      121 cctctttttg acgattatga tacaaaaaac atcatttatg aacaaaatgt gaataacggg
      181 cgttcttgag atatgatttt aattgggaaa aacttatcga taaggaggtc tttatgaaaa
      241 aaagactgtt atggtgtttg ctgtttctat ccctaagttt aacaagtcag ccagtttttg
      301 ctgaagaaac acagcggtct cctattacag gcacacgtct gtacaaaaat gtttatgtac
      361 agacaacaga tcttgattct gcgattcctg aaacagaaga acggtctttg cagccaagat
      421 ttggttactt ttggtactgt ccgacctgtg attatgaaag ttgctggcat atcttacatt
      481 ctacagctgc agcttttgct tttactcata ctcagaaata tgatggacat catcctgtta
      541 tttatgaggt atagatatga gcaaaaaaga aaaactaatc tcgttgatcg tattggtttg
      601 ccttgttctt ctggtaactg tcataggcta tcagaaatac catgagaaca tcagacctac
      661 taaagaatct tccccagcct cgaccgttga aacagaaccg ataagtagtc agaaagaata
      721 atttttttag tttaaaaaag aacatcagag caaaaaaaca ccaactgaac aatatgtcgt
      781 ttattttaaa aaaagttcgg tttttcgctt tatgtaatcg ctgagaaaac tttttttaat
      841 aaaaaaagtc gaaaaactct agcttttttg taaacggtat gatatactag aggagtagtt
      901 tttgttcggt aatctgtggt agcttgtttc aaataaacat cttccagata tacaccattc
      961 aagtaattgc aaaatatgtg ttgatacaca aggaggattt ttattatgga aacaggtaca
     1021 gtaaaatggt ttaactcaga aaaaggattt ggattcatca ctgcagaaaa cggaaacgat
     1081 gtattcgttc atttctcagc tatccaaggc gacggattca aaactttaga agaaggccaa
     1141 gcagtaactt tcgatgttga agaaggccaa cgcggacctc aagcaacaaa cgttactaaa
     1201 gcttaattaa cctatactaa aaaataaacg cccagacatt tgcgtgtctg ggcgtttttg
     1261 tctattttct ttaaaaagcg atgagagtat ttttctgcac tttgtagtat aatttcaaaa
     1321 gaacattaga ggcaggagaa gaggaagata ttttgaaacc aagaacttat gcagtcgttg
     1381 atatcgaaac gactgggacg aatccaaaag aagatcggat catccaattt ggctgcgtca
     1441 tgattgaatc aggtcggatc acttcacgtt tttcgatcga tattcatcca gggcggaaga
     1501 tttcaaaaca aatccagcat ttaacaggga tcacgaatca acgcgtccaa aaagcgcctt
     1561 atttcgaaga tgtcgcccaa acgatttaca atcttttggc agacaccata tttgtcgcgc
     1621 ataatattta ttttgattat aattttttga accatgaact catgcgatgc ggacttccta
     1681 gtttgaagat accaggcatc gatacagtag aacttgcaca aattttcttg ccgacggaac
     1741 caagctttcg cttagctgat ttgtcagaaa gccttggatt tattcatgag aatcctcatc
     1801 aggcagatag tgatgccgaa gtgacaggtc aacttctttt gctgatcgaa gaacggatga
     1861 gaaaattgcc aatcatcacg ttagagcaga ttgcgcgatt aagtagacac acaggtatgg
     1921 atacgagtcg tttcatttat catgtgattg aagaaatgaa agaaaaacca gagccacttg
     1981 atccctcgtt agaaatcgtc gatgggctag ctttacagaa aaaagaagtg gaactattca
     2041 catccgtcca ttatggcgag cggacatatc caagaaaaaa acaagcaaaa gaaaaaatgt
     2101 ttggtaaaac actgatgtat cgaaaagaac agaacagact gatgaatgcg gtctacgacc
     2161 atttcacaaa agatgaatca aaagatttga tgatcgaagc agctacagga atgggtaaaa
     2221 caatcggtta tctgctacca ttgagttatt tggctacacc tgaaaaaccc gctgtcatca
     2281 gtaccgtttc gctagttttg cagcagcaga tcatagaaaa agacattcca ttgctgaatc
     2341 aattgctaga tcagcccata caacctgtaa ttatcaaaag ctatcgccac tatatcgacc
     2401 tgcaacgatt taaaggaaca cttgtggaac cgccagaaca aaaacaatat gctttgtatc
     2461 aaatggcggt cttagtttgg ctgacgcaaa cgaagaccgg tgatttggat gaattacatt
     2521 taacaaattt gaaccattca ttctttgcag atatcgcgca cagaggtatt ggatttttag
     2581 caagaaatca gtcattttac gaacaagact ttgttcgttt cttgcaaaag aaaatcaggc
     2641 aaagtaattt tttgattgtc aatcatgcgt ttttagtaca agaaacacag cgaaaagaac
     2701 cattgcttcc agctagtcca tttttgttga tcgatgaagt acatcatttg ccggaaatcg
     2761 ctgaaaaagt aaatgacaaa cgattaagta ttaactattt ccataaacaa atccagcaat
     2821 tagaagaacc aaatcagctg tttgaccgaa tacaaagcta tttaccgaaa gaacatgaag
     2881 ccttacgctg gttatcgatt tatcgagaag aactttctgc gctatgcgaa gcacagattt
     2941 gtatggcaga aggattaatg agtttagtga gcggcaagca acccaacgga cagtatccag
     3001 aagaagtcat gatgacaaaa gagatgttgg atcagctgcc actggaatct gggacagcca
     3061 ttgatcacct gcagcttttt tatcaggaga tccagcagct acagtcacaa atgcgtctga
     3121 cttttgagat tatccaagaa agctggacaa accagatgcg gatcgattta gcaagtttat
     3181 atagcctatt tgatcaatta gacaagcagt acttgctgat ggatcaatgg ctggaagact
     3241 ggcgagatca tttgatccat tggttcgttc ctgggaacac agcgcagcag atgatttttc
     3301 atctccatga ttttcaagcg gctatgttgc cgtcgactgt ttggtatcct cgatatgatc
     3361 gtattcttta tattggagga acgctgaaga tcggccctaa tcgacactat ctctctaaga
     3421 ggcttggcat tgaagcagca cctcttaaag tgattgcctc tccttatgat tatgagaaac
     3481 aggcaaggat ctatgtgcca aaagaggcac ctgccgtcca tcaagtcagc aatcaagcat
     3541 atatcgatta tttagagaca gcacttacag gtctgatcga acaagaagat cggccaatac
     3601 ttgtgttatt cacttctcat gatgttttgc agaaggtcta tcagaaaatg catctgtcta
     3661 tgctagaaaa aggacgagaa atactagctc aagggtttgg cggaagccgg gagaagcttt
     3721 tgaaacgctt cattttgtca gaaaaaagcg tattgtttgg cgctgacagt ttttgggaag
     3781 gtgtagattt gcctggagat gctttgcaga tcgttgtagt cacacgttta cccttcgata
     3841 atccgcttcg cccgatggtc aaagctagga acgcttattt agaatcagaa gggatcaatc
     3901 ctttttacca ggaatccgta cctaaagcaa ctttgaagtt acgccaagca ttaggacgct
     3961 tgataaggac ggagacagat cgtggcgtat tgcttatttt ggatcgaaga ctagttactg
     4021 caaaatatgg tgcacggatc atccatgcat taccgaaaaa agtaccgatc aaagagctag
     4081 ctatggagga aatctatcag gatatccatg aattcttaaa gaaaaatgaa gaaggaagtt
     4141 agaggagctg tcttttttca taattaaaat ctgctataat gaaaaggaat gaagataaag
     4201 gagcgacgac ccttgaagaa aaaacagaca aaggaaaaaa cgattaatcg cgtgttactt
     4261 ggcttgatcg tatttttatt agcggtcatc gtgatcagct cgatttttta cttgcgttcc
     4321 agtcgtccgg cggtgcaggc gaagaaagaa gcagtagaaa tcgcaaagaa atatgcaaat
     4381 cttgaggaag ccgatcattt ttattggttt actcgaaaga agacatactt ttcactgaca
     4441 ggtacaaatg ataaaggaaa agaaatcgcc gttatcattc ctaaatctgg tgaaaaagta
     4501 aaagtattgg atcaaacaaa gggactgact gaaaatgaag caaaacagac aattgctgcg
     4561 gcccatcctg aaatccaaat cgaaaaagct gctttaggga tgtatgatga tcagcctgtg
     4621 tgggaagtag taggaaaaga aacaaatggg aaactgaatt attacctgat tggctatgaa
     4681 tctggagatg aagtcaagac aatcaaggga atttaaaagg taaaggaatg aatgggatgg
     4741 agttatcaaa aagaacaaaa cgattagaac cgtctgtcac tttggcagct gcggcaaagg
     4801 ccaaggcatt aaaagcaaaa ggcagagatg tgctgagttt gactgtggga gaaccggatt
     4861 ttgcgacacc agaaaacatc caagaagcag caatcgaagc aatccgcaat gggaaagcta
     4921 gctattatac accaacagca ggaatcccag agcttcgaca ggcaatcgtt gattatctga
     4981 agaaaaacta tggacttgtc tatgaacctt cacaaacagt cgtaacagac ggagcgaaat
     5041 ttgctttgta tacccttttc caaacgatcc ttgacccgca agatgaagtc atcattcctg
     5101 tgccttactg ggtcagttac ggtgagcaag taaaactggc agaaggactt cctgtctttg
     5161 tgaaagggga agaatccaat agctggaaag ttactgttga acagctagaa caagcacgtt
     5221 cagaaaaaac aaaagccgta atcatcaatt ctccatcgaa tccgactgga atgatttata
     5281 gcaagaatga actgcaggca atcggtgaat gggcagtaaa aaatgatatt ttgatagttg
     5341 ctgatgatat ttatggccgt ttagtttata atggcaatga gtttactcca attgcaacga
     5401 tttcagaagc aatcaaaaat cagaccatca tcatcaacgg cgtgtctaaa acatatgcga
     5461 tgacaggctg gcgcattggt tatgcggtgg gaaatcctgt gatcatcaat ggaatgatcg
     5521 ctgttgcttc ccaatcaact agcaatccga cagcagtcag tcaatatgca gctgttgaag
     5581 cattgaaagg tgagcaagat acagtagaag aaatgcggaa cgcctttgaa gaacgcctga
     5641 atacgttata tccgcttgtg gcagaactgc cgggtgtttc tcttgaaaag ccgcaaggag
     5701 ctttctatct ttttcctaat gtaaaagaaa cgctacgcat gtgtaagtat gaaaatgtca
     5761 cacaatgggt agaagatctt ttagaagaaa caggggtagc gcttgtgact ggcgaaggct
     5821 ttggggcacc agaaaatgtc cgcatgagtt atgccactga tcgcgctacg ttagaagaag
     5881 cggtacgcag aatcaagcaa tttattgaga gtaaaagtca aaattaatcc tgaagaaatt
     5941 caaggaatat aaaaggagag tcatacgtgg aacaaattca aatcatcgat tcgaaaaacc
     6001 atgtgggaga aacagtaaca atcggcgctt gggtcgcaaa taagcgatca agcggaaaaa
     6061 tcgcattttt acaattaaga gacggaacag cctttttcca aggtgtagtt gtaaaaagcg
     6121 aagtgtcgga agaagttttc caaacagcta aaaatctaca ccaagaaaca tccgtgttag
     6181 taactggaga aatccgtgaa gacagtcgtt caaaatttgg ttatgaaatc ggaattacaa
     6241 atgtggaagt ggtaagcgag agccacgatt atccgatcac accaaaagaa cacggaacag
     6301 atttcttgat ggatcatcgt catttatggc tgcgttcttc aagacaacat gcgattatgc
     6361 agatcagaaa tgaaatcatt cgtgcaacgt atgaattctt taatcaacat cattttgtca
     6421 aaatcgatcc gccgatcttg actggttctg caccagaagg cactacggat ctattcgaaa
     6481 caaattactt tgatcaaaaa gcttatttat ctcaaagtgg ccaattatac atggaagctg
     6541 ctgctatggc tttcggaaaa gtcttttcat tcggtccgac tttccgtgct gaaaaatcaa
     6601 aaacacgacg tcatttgatc gaattctgga tgatcgaacc agaaatggct ttcactcatc
     6661 aagaagaaag tttagaagta caggaacaat atgtagcatt cttagtacaa aatgttttag
     6721 atcattgcga atatgcatta gatgtactag gacgagacaa agaagtctta aaaacttata
     6781 caaaattgcc attccctcgt attacttacg atgatgcaat cgaattactg aagaaaaatg
     6841 gatttgatga catcgaatgg ggcgaagact ttggttcacc tcatgaaacg ttcattgcga
     6901 attcattcga acagcctgtc tttatcctaa actatccaaa agccatcaag ccattctaca
     6961 tgaaaccaca tccaacaaga gaagacgtgg taatttgtgc agatatgatc gcaccagaag
     7021 gttacggtga aattattggc ggaagcgaac gggcaacaga ctatgatttc ttgctagaac
     7081 aaatccgtaa tcacggtttg gatgaaaaag aatattcatg gtatcttgat ctacgtaaat
     7141 acggttctgt tccgcactct ggttttggtc taggccttga acgaacagtc acttggattg
     7201 ctggtatcga ccatgtacgt gaagctattc cattcccacg tttgttgaac cgaatttatc
     7261 cataagaggt gatgaaaaag ctgtttagtt tcgagtatta agaaaaattt tgtg
//
LOCUS       DACECR010000124         6852 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.125, whole genome shotgun sequence.
ACCESSION   DACECR010000124 DACECR010000000
VERSION     DACECR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.125"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(45..1022)
                     /locus_tag="IWO17_002542"
     CDS             complement(45..1022)
                     /locus_tag="IWO17_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ0291396.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="IWO17_002543"
     CDS             complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="IWO17_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ0291397.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            complement(2595..4019)
                     /locus_tag="IWO17_002544"
     CDS             complement(2595..4019)
                     /locus_tag="IWO17_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0291398.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(4283..5260)
                     /gene="guaC"
                     /locus_tag="IWO17_002545"
     CDS             complement(4283..5260)
                     /gene="guaC"
                     /locus_tag="IWO17_002545"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ0291399.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            complement(5517..6722)
                     /locus_tag="IWO17_002546"
     CDS             complement(5517..6722)
                     /locus_tag="IWO17_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0291400.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
BASE COUNT         1933 a         1526 c         1161 g         2232 t
ORIGIN      
        1 aatttaaatt gataagccat gactttgttt cgtcccttct tttcttaaaa ttccgcgatt
       61 tcttttactt tgtcttcgat atcttttgca tttggcaacc agacattttc tgcttgtcca
      121 aatgggaaga ctgtatcagg tgcagaaact cgtccgattg gggcttctaa tgaaaggata
      181 gcgcgttctg agatctcaga aactacttgc gcggcaacgc ccgcttgtcg ttgtgcttct
      241 tggacaacaa ctacgcgacc agttttttcc acggattgaa tgatcgtttc tacatctaat
      301 ggcgcaactg tacgaagatc aatgatttca acagatatat tttcttttgc aagattgtct
      361 gctgctttga ttgcttcacg caccattgct ccgtatgtga taatagatac atctgttccc
      421 tcacgagtaa ctgctgcttt atccaaagga acttcgtaag cttcatccgg cacttcttct
      481 cggaatgagc gatatagttt catgtgttct aggaaaacaa ccggatcgtt acttctaata
      541 gaagaaatca atagtccttt tgcatcataa gggttggaag gaatcactac tcgaatacca
      601 ggagactgag cgatcaaacc ttctaaatta tccgaatgca attctggtgt atgaacaccg
      661 ccaccaaatg gcgaacgaat tgtgatgggt agatttctag tgccgctcat tcgatatctt
      721 gtacgagcca tttgagctac tacttcgtcc attgcttcga aaataaaacc aaagaattgg
      781 atttcaggaa ctggacgaaa tccttctaac gcaagaccga aactcaatcc tgcgattcca
      841 gattcagcta aaggagtatc gaatactcgg tcttctccga acttttcctg caatccttca
      901 gttgcccgga aaactcctcc gtttttccca acatcttcac caaaaacaac gacattctcg
      961 tcattttcta gttcaagtgc taaggcatct gtgattgctt ggatcattgt tttttgtgcc
     1021 atgattattt cgactccttc gcttcataaa ttgcaatttg ttctttgatt gattgtggag
     1081 atacttcgaa catgtttttc aagaaatctg aaaccttttg ttttggtact ttgtcggctt
     1141 cagcaatagc tgctttgatt tcttctttag ttgcttcgat gacttgttct tctttttctt
     1201 cagaccaaag tcctttttcc gtcaaatatt tacggaagcg gatcaatgga tcttttttct
     1261 gccaatcgtc atctgtgtct tttgtacggt agcgggttgg atcgtcacca gatagtgtat
     1321 gtggtccata acgataagtc agtgtttcga ttaggactgg tccatttcca ctgatcgccc
     1381 attcccgtgc cattttagat acagtatata ctgcaagcgg atccattcca tcgacttgaa
     1441 taccaggaat cccagctgcg acagcttttt gtgcaagtgt ttttgctgca gtttgttttt
     1501 cacgaggtgt agagatcgca tacccgttgt tttggatata aaatacggca tttgcatggt
     1561 aagcgccagc gaaattgatt gcttcgtaaa aatcaccttg tgatgaaccg ccatcaccag
     1621 tataagtgaa gacgacattt ttcttgtttc gtttcttcaa acctaatgca acaccggctg
     1681 cttgtacata ttgtgcaccg atgataattt gtggtggtag agctttcaat tcttccggat
     1741 aagaattacc tgcagcatgt cctcgagacc ataaaaaagc ttgagataaa ggtaaaccat
     1801 gttttactag ttgaggtaca tcccgataac ctggaagaag aacatcttct ttatcaaatg
     1861 cgtaagcact agccagctga cttgcctctt gtccagcagt tggtgcaaaa aagcctaggc
     1921 gtccttggcg gttcagcgct gttgaacgtt ggtctaatac acgagaccat accatatgag
     1981 tcattaattc aacgagttca tcatcggata aatcaggtac taaatctttg ttaactacgt
     2041 ttccatcttg atcaagtgct tgatatactg ggaaatctgc attgacttca tctagcagtg
     2101 cttggaagtc gatcggtgct tttttgtttg ccatttgtca cacattcctc tctcatctat
     2161 aagaaaatct cttcattctg cgattgaaac agtgaataaa actgtatcac tatttaaccg
     2221 tcttcattaa taaattatca tggatttatg agaaatacaa gctaaaagca caaattttca
     2281 cgatgaaagg aaattatgcc aatccgcata ttaacagcat tagtgaataa aatcacaata
     2341 aggaaagcgt tttatatttc atctgcattt tttctatttt ctaatcaaac ttacaaccat
     2401 ctgtttctat ttaaaaacgt tggtgttata cagttttgtt gcaaacattt attttattta
     2461 cacatcgtga acggacgtac aaataatggt tttttcttag tttctttata tcgtattctt
     2521 tttccgaaaa aaaagaaaca gcttgaaatt taaatccaag ctgttatatt ctttaatcta
     2581 tttactttgt gaaattattc tgttaaccac ttttctagaa ttttcatctt tgaataaaac
     2641 aacggtattt ggttttcatc cagcctactg taattgattc tgattccatt tatatcttgc
     2701 ccaaaaagta gacttggtgc aattccaaga cccatatcta ctactttttc tgctagttcg
     2761 gggtctagtt ttttcccatc ccaagtaatc caggcatagt aaccacctaa aattgaatga
     2821 agcttgaaaa acgattgtga atctccccaa gcgcttatca gtcggctttt ttctttcaac
     2881 ttttttctta catcttgcat tttcaaatca attgcttcat taaatacatt agccactgtg
     2941 atttgtgtaa ggatattcat cgaaaactcc atcatttctt gcgcttgagc tagttgcgag
     3001 ataaaagtct caggtcctat cacccaaccg attttcgttg tttttcctaa aaccctagat
     3061 aacgaaccga cgtataacac attatccgga tcaatcgttc gtataggaag gacttcttct
     3121 tggtttagaa atgaaagatc cgtaaataca tcgtcttcaa taattggaag ccgatactgt
     3181 cgacacaatt ctactagctt ttctcgtcgt ttttgactca tcacttttcc tgtagggttt
     3241 tgaaaattcg ggttgataag cactgctttc aatttatttt tatggatcga ttctttcagt
     3301 ttttctaagt cgatcccttc ttcatccatc ggtatcccaa ataaacgcgt tcctgaagct
     3361 ttgaaaagag gtaatcggta aaaaaaagaa ggtgactcta cagctatccc ctctccagct
     3421 gaaagaatcg tttgtaaaat caaaaagatt gcttgctgac cgccaccagt cacaagaagc
     3481 tggttggggg aaaatgccaa ttcaaattta ctttccaaat accgtttgat tttgttacgt
     3541 aacggagtat agcctaaaag atccatttct ttttcttccg ctaagatttc ttcaagagaa
     3601 taagaaggaa actcaaaatt cggcaaaagc tcatctggca tctcgctagc gtataaatca
     3661 agaaacgatg ttttttggat ctgctttgct tctcttattt tttgctcata ccagtccgtc
     3721 tgctcataac gaggagagat catttgccgc caatcgattc ttggaacagc aaatttcccc
     3781 cattcagtct gattaacaaa ccatccgctg ccacgtttgc tcgttagtac cccaagctcc
     3841 ctaagctctt ccaatgcatg gacaacggtg gaacgattca caccaaaaag ttgccctagt
     3901 tttcgttctg attcaagttg acttccagga ttgatccttc cgttttcgat ttctctcata
     3961 atctgttcca taatttgacg ataaatcggt ccttgttttt tctctacttt ttcccacata
     4021 agcttctctt ttctattgtc tattttttct tttgatttta ccgaatttac gagaaaaaag
     4081 aaaaactttt tttgcaaaac ataagggact gtgacaaaaa tcttatcaca gtcccttatt
     4141 ctgaataatg atgttcaaaa gcttgtatct gcgataataa ttccagccaa acaaaacata
     4201 taaagaacta tttttgtttg gctgttctta ttacttaatg caggacaact ttttcacaac
     4261 atcctaatat tttatttatt ttttagattg tgtctccatt aaaaatcgaa tttttcacga
     4321 tgacatagtc aacttttcga atggcttcca gatctcttcc tccagcataa gaaatagatg
     4381 attgaaggtc ttgttgcatt tccactagtg tatctttcaa agatcctttg tgttggatcc
     4441 agattttctt tccttcaaca tttcgttttt cgcctttttg gaattctgaa gcagaaccaa
     4501 agtattcttt atacacgatt ccattttcga cttttgtttc gccaggagat tcctcgtgac
     4561 cggcaaacaa tgaaccaatc atgaccattg tcgcaccaaa acggacagat tttgcaatat
     4621 cgccatgtgt tctgatccca ccgtcagcga tgatcggctt gcgagctgct ttcgcacacc
     4681 atctaagagc tgccagttgc cagcctccag tcccaaagcc tgtcttgatt tttgtaatac
     4741 atacttttcc tggaccaatc ccgacttttg ttgcatctgc gcccgcattt tccaattcac
     4801 ggacggcttc cggtgtacca acattaccgg caatcacaaa agtttcaggc aaatattttt
     4861 taatatgctg aatcatatca ataactgagt tagcatgtcc atgcgcgatg tcgatagtga
     4921 tataatcagg atttagcgat tcttctgcca acttctctat aaaggaatat tcctctttct
     4981 tgacgccaac actgatcgag gtaatcaatc cacgcttttt catttttttg ataaacggaa
     5041 ttcgagctgc ttcatcaaac cgatgcatga tatagaaata accattttcc gctaagaatt
     5101 ctgcgattgt ttcatcaata atcgtctgca tgttggcagg cactactggc attttgaatg
     5161 tatgtttacc taatgtgacc gttgtatcac attcagaacg gctatttacg atacatttat
     5221 taggaattaa ttgaatatct tcgtaatcaa atactttcat tacattatcc tcctgatgtt
     5281 cacatccagc aaaacacgaa cattattaat ttaatcaagg ctttttttag cgccctatgt
     5341 aatttaactc aattaagcaa aaaaatcaag agcaaaaaga gaatttatac gaatggtaaa
     5401 aaaacatata ttatttttag tctatacaaa cgaaacaatg aatgttcgct tttagcgaaa
     5461 aaaagactgt gacaaatgtc ttgtcacagt cttttttctg aataaccggc gttctcctac
     5521 gttgttcgtt ttttcttatt atacaagtct agcacaacgg ctaaaagcaa gatcaatcct
     5581 ttgattgcct gctgccagtc taccccgacg cctaaaatcg acatgccatt gttcaataca
     5641 cccatgacca agccaccgac aatcgcaccc atgattgttc caatgccgcc ggaagtcgaa
     5701 gcaccaccaa aatatactgc cgccattgca tccagttcca tcgatgtacc tgcttgaggt
     5761 gttgccgcat tcaatcgagc agctaaaact aatccagcta aagctgccat gactcccatg
     5821 ttgacgaata cccagaacgt gattcgcttt gttttgatcc ctgatagttc agccgctttt
     5881 cgatttccgc ccgtcgcata aatctgtcga cctgctactg ttttatttgt caaaaaccca
     5941 tatgctgcaa cgatcaaacc aagaatgatc aaaataatcg gatacccttg ataagaggca
     6001 aagatataag ttattcctag tacaacgact gctgtaaata ccgcctttac caagaaagct
     6061 gttggagatt ccacttcaaa taaatgattt ttccgacgat ttcttgttcg ccattgacca
     6121 acaactaaca cgatcgtgat gatcattcct aaaatcaatg tcaagaagtg aaggctgctt
     6181 ccttcgctta agtctggtaa ataacccgta gacatttttt ggaaaacagc tggaaaaggt
     6241 gctagagatt ggcctcctag aacaacttgt gtcagtccac gaaacatcag caatcctgct
     6301 aaagtcacga taaacgccgg tatccctgca taagctaccc aaaaaccttg ccatgcgccg
     6361 atcagcccgc ctactgcaag acatgcgata actgtcatcc acgggttcat ttggcgattg
     6421 accatcatca tgcccgcaat tgcaccaaca aacgccatca ctgaccctac cgacaaatcc
     6481 acatgaccta agagcacaac taataacatc cctgcagcta gtactaatat atggctattt
     6541 tgcagaacaa ggttagttat attcaatgga cgccagaaaa taccatccgt catcaattga
     6601 aacgcaataa gtagtcccgc taaaatgatc accatgctgt agcgaccaaa aagatcagct
     6661 gctctacttt tcaaatttag ttttttctct tttttatccg taagattttt tgcctgttcc
     6721 aacttaaccc acctcttctt tcgtcattaa tttcatcaag acttcctgat ctgcatcttc
     6781 tcgcgagact tctcctgtaa tacgtccttc gttcatcgta tagattcgat cacacatccc
     6841 taatatttca gg
//
LOCUS       DACECR010000125         6842 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.126, whole genome shotgun sequence.
ACCESSION   DACECR010000125 DACECR010000000
VERSION     DACECR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.126"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(198..1316)
                     /locus_tag="IWO17_002547"
     CDS             complement(198..1316)
                     /locus_tag="IWO17_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291401.1"
                     /translation="MAKKDKELLSQLISEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FSKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1789..3096
                     /locus_tag="IWO17_002548"
     CDS             1789..3096
                     /locus_tag="IWO17_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0291402.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKVSGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            3214..4413
                     /locus_tag="IWO17_002549"
     CDS             3214..4413
                     /locus_tag="IWO17_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291403.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRVIFSSNNYHIF
                     RAGLYACQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4440..5708)
                     /locus_tag="IWO17_002550"
     CDS             complement(4440..5708)
                     /locus_tag="IWO17_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ0291404.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DTRLSHPWLLYLLPVSGALFAYLYVYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(5871..6518)
                     /locus_tag="IWO17_002551"
     CDS             complement(5871..6518)
                     /locus_tag="IWO17_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ0291405.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELLEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
BASE COUNT         2070 a         1412 c         1227 g         2133 t
ORIGIN      
        1 caaacaaaaa atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag
       61 ctttttcaca acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa
      121 aacggaaaaa tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac
      181 tgatttcaca acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca
      241 tctttttgat ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca
      301 ttcaacactt gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag
      361 tttgacgtag cgataaataa atgtgacact tttccttctt ttgcagctgg aacgatatca
      421 actaactgat cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct
      481 gcgagttcac ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca
      541 ggagaagcag aaatcgcaac gtctgcacaa tagtaaggat ttttggcgat ttttttgaac
      601 atcgtctgat tttctggtag agcaaataaa taaatcggcc attgttctgg attttcgaaa
      661 gaatccttca agtacgtatc aactgcttgg taatagttga cccaatcgat ctctacttct
      721 tcatcttttg gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta
      781 gaagagaagt tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc
      841 ggtgcccctt ctggtagaga tacttctttt acttgtttgt tgctgacttc atacaatttc
      901 atggagtcgc ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata
      961 atagccagta aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa
     1021 cgatggatat aggtttggtc agcagttata atgaccgcta cactcgtagt tgcactacgc
     1081 caaaacgaag gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt
     1141 tcggaatatt ttttctcaaa acgtttcttt gcttctttcg aaaaattttt gaatttgatc
     1201 tgatcttttt ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt
     1261 cctttagcgc cttctgaaat caattgtgac aataattctt tatccttttt tgccataaac
     1321 gtacccctcc taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt
     1381 ttccatacaa taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct
     1441 atttttttaa gttatcttca taatttcctc aattatttag ccccagtctt caactattct
     1501 tcatgaaatg ctgataaact aagaaaaaga ataaaagagg tgtatcttat gcatttgatt
     1561 atgattttca gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta
     1621 gaggcggaaa cacagcctgt tcctataaaa gtacgaaaaa atgaccgcta accttttcat
     1681 aacgcgcgtt cttatcctaa agtttttttc agaaacattg aaactctctt ccttctctga
     1741 taaaatttta atttatagcg aaacaaatta aatacaagga gatcggatat gtccataaaa
     1801 aaaacacgtc tagtcctatt attggctagt ctcattcttt gccttgcttc ttttctacca
     1861 gctgttgctg tgcaagctga agatacgttc aaagtaaatg caaaagcagc ttttgctgtg
     1921 gatgcgcaaa gcggtaagat tctttatgat caagatggag aaaaaaccat ggggatcgct
     1981 tcaatcacaa aaatcatcgg tctttacatc gtccttgatc aagtaaaaga aggaaaacta
     2041 tcatgggatg ataaagtttc gatttcagat tatgcagaaa atttgagtat caccccagat
     2101 ttatcgaatg ttcctttgca taaagaaaac acctacactg tcaaagaatt attcgactct
     2161 gcaatcatcc agtcagccaa tgcttcaatg gtggccttgg cagaaaaagt ctctggcagc
     2221 gaagctaaat tcactgaacg aatgaaagaa caattgaaag attggggaat caaagatgca
     2281 acgatcgtta atgcatcagg attgaacaac tcttatcttg gcgaaaaccg accagaaggc
     2341 actggagaaa acgacgaaaa tcaaatgtct gctcaagatg tggccatcgt tgcccgccat
     2401 ttgatcttgg atttcccaga aatactggat gtatcaagta ctacgaccca aatgtttggc
     2461 gaaaatacgc aatcaccagt ggaaatggtc aactggaact ggatgctccc aggattcgtt
     2521 aattacaagg aaggcgtgga cggattaaaa actggtacga ctgagcttgc aggtgcttgt
     2581 ttcgttggta cgatcactaa aaacgatcaa cggatcatca ctgtcgtatt gaatgcggac
     2641 ggccatgcgg aaaacccgag tgcccgcttc aatgaaacag accgtttgat ggactactgc
     2701 tatgacaatt ggtcagaaaa agagctcgga aaagctaacg caagtattcc tagcctaaaa
     2761 accattgatg taaaagacgg aaaagaatca acagttcctg ttgtgcttaa aagccctgta
     2821 aaagtatggg tacgaaacga tatggatact ggcaaattaa cgatcacgcc aacacttgac
     2881 aagaagcaag tcactgacaa cgagatccaa gcacctatgg ataaaggtac aaaaatcggt
     2941 accgctacga tcaccttggc agacgataaa ttaggttatt tagaagataa caatgcgcca
     3001 tcgacagaga tcattacgga taaaacagtg gaaaaagcga atatgttcgt gattggctgg
     3061 cgacgcgtcg cagaattttt cggcaacttg ttttaattga acaataagaa aagcatcttt
     3121 ccatcggaaa gatgtttttt tgctttttct agtttaataa cagctttttt catctatttt
     3181 cgttatactg tataagaaaa aggagttgaa tttatgaatc taaccagaga tctgatcttt
     3241 ttagttctct atcttattgg ggtattctct atcgtttatt ggcgaaaaca gaagaaggat
     3301 ccttccttgt tcttttacgg cagtacgtgg acatttacgg taatcttaac ttattttgtc
     3361 gtgttggaaa ttttttattc gaccttttat tttttgattc cattgttgtt ctttagtatc
     3421 tttgcttttt cttatttcac agaaaaacga aggctgctga atggtctttt attcaatgtt
     3481 ttcctcatca gtttcggcat ctacttgttt gtcttgctgt atgaaacgca aaatattttc
     3541 cttggcggac tgatcgcctt gatcacgatt ccattgctac ttgtcctttt atttggtatt
     3601 tatggattga ttgtttttct tttttggaac ggggtcacag ttttacgtag agaatcgcat
     3661 tcattagcta atcttttaac gttgatacta gccatttttc ttacactgtt tcttgttttt
     3721 gactttttct tgttaaaata tttgccgcaa tggataaatg ctttgttttt ctgcgttccc
     3781 ctgatcttga tctatctatt tatcgtattc tacaactttt tgactgtctc attcttgtat
     3841 cagttcaacc ggcctcgata caatcaagac ttcatcgttg tgttaggcgc tgggttgatc
     3901 aatggagaaa cagtttctcc tcttctagcc aagcgaatca ataaggcgat tgctttttat
     3961 cgggctcaaa gccgtgctac attaaatccg cccatcttat tgatgtctgg cgggcaagga
     4021 gcagacgaaa aagtaccaga ggccattgcg atgaaacaat atgccatgga acaaggaatc
     4081 cctgaaagag acattcttgt cgaaaccaat tcgacaacca ctttggagaa catgctctat
     4141 tcaaaggaaa tcatggatca gcaaatgaaa gggccatacc gcgtgatctt tagttccaac
     4201 aattaccaca tttttagagc cggtctttat gcgtgtcaag ccaaattgaa agcaaatggt
     4261 attggtgcaa aaacagcttt ctactacttg cctaatgcgt ttttaagaga atacatcgcc
     4321 atcttgatga tgaacaaaaa aagacatatg atcatttgcg gattgctttt cgcaggctcc
     4381 gcattcttat cgctgattac tttacttttc taaaaaaaca aggtgtgaca aaacatatgt
     4441 cacaccttgt ttttttcttt cattctctta actattggca aaaagagtat cccttttttg
     4501 accgctactt gctgagcaga ataaataccg ctgttgcctg aacacataaa gctgatcaag
     4561 cagatcataa agaagtatac tgcagcttgt gaaccgaaca attcgatccc catcacaaag
     4621 caggcaatcg gtgtatttgt cgcaccagaa aatactccga taaagcccaa tcctgcaaga
     4681 aaaggaacag aaacatggag caaaggcgct agtgctgcac caagcgttgc accaatttca
     4741 aacaatggtg tcacttctcc cccttgatat cccgcgccta aagacaaaac tgtgaagaat
     4801 aacttcccaa taaaatcata gacatgtgcc tgtccattaa atgattcttg aagtaacgga
     4861 agactcaatc cgagataacg ttgtgtttgc aaaatcaatg ctgctgccac aactacagct
     4921 ccacctatcg tatttcgcca aacaggatga ggaatccatt gtgcatatcg agcttttaaa
     4981 aagacgatcg aacggctgaa tacccagcca atcaaaccaa aagcaatccc agcaacaaaa
     5041 actttgaaga acagcaacat actccattca ggaatcacac ccatttggta atgtagatga
     5101 gaaacaccat agctttctgt catgaaattt gcaaacagtg cagcaaagaa gctagggaag
     5161 atcgcttctg ttcggacttt tccgattgcc aacacttcta atccaaataa tgttccagct
     5221 aaaggtgtgc caaatacaga actaaaaccc gcactgattc cactgataat caagatttcc
     5281 cgttctgatt tatccaatcg gaacaaacgt gctgtattat ctgctaacgc tccccccatt
     5341 tggactgctg ttccttcacg gccaacagat cccccaaata aatgggtagt tatggttcca
     5401 aacagtgtaa gtggaataag tcgcaacggg attttttctt ctccaccatt tccttgatcg
     5461 atcactaagt tgtttccacg actagagaga ccgccgtgat acacatagag ataagcaaaa
     5521 agcgcaccac taactggtag tagataaagg agccaaggat gtgatagtcg ggtatctgtc
     5581 acaactgtca agctatttaa gaaaaaagca gataagctcc ccatgaatat accgagaatc
     5641 gctgtgatca ccagccactt cagcatatac actaaaatgc gcattggact cggtaattgt
     5701 ttttccataa aaaattcctc ctcaataaaa aaacgcctac taaagaaagg ggctgtcccc
     5761 agactttagt aggcgtcatt agctatcttt cgttagcatt taaaggcgaa caccatcacc
     5821 tattcacttc cattaaaaga tagcatattt ttctgtaaaa aacaataaaa ttatcgttct
     5881 ttctttacac gttcagcaaa ttcatagaaa ggagccaatt tcgttaactg ttgattacat
     5941 tggtcactat catgacaaat ataattcccc tttttcgtat acgtgccatc agaacctgct
     6001 ttcgttgtag aaagaaaaag cgaaacattc gatgttttct gacagattgc acagattccc
     6061 ttatttatcg ttggtgataa agtaccacta atccctttta atttttcatc ttcgtaatag
     6121 accatgaatt ttttctgcgt acctggatca ttccagccga tgtacgtatg ttcttgcaag
     6181 tcgatttcat tccaagctgg ttgtttgagt tttttcactt ttcggaataa tttctcgatt
     6241 tgtggtttgc taggctgcat aaacggctga acgatttgct tcagctcctc caatgctctt
     6301 tctgcttgcg cttttgtcag ttttacgtct aaaactgtcg ttaaaaatgt atcgatttct
     6361 agcagttcgt gatctaatat gtctcgaatc ttttcagctg ctagtgcctg aactgtttgt
     6421 accgtttttg gatcattgac agatgagtac gcatttaata actgttctac ttctcttttt
     6481 ataaaaaaat attggtatgg ttttattgtt tgttccatga ttttaaagtc ctttcttcta
     6541 accaaagtgc atactttgta gaaaaaagtg atcttctgca aagtatgtgt tacctgaaga
     6601 tcccggactc ttttaaataa gatacgtttt gcatcttatc atcaccccct ttaatgctta
     6661 ttcttctatg tacatgattc attcgataaa taaaagtaaa ccgtatcttg aacagatagt
     6721 caactgcaaa cagaaaaaag gactgtgaca aaagtcgtgt caaagtccct tgtttcgaat
     6781 gaatggtgtt caaaagcttg catctgcggt aataagccga aacttcacaa aaatttgaga
     6841 ag
//
LOCUS       DACECR010000126         6734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.127, whole genome shotgun sequence.
ACCESSION   DACECR010000126 DACECR010000000
VERSION     DACECR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.127"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            933..1607
                     /locus_tag="IWO17_002552"
     CDS             933..1607
                     /locus_tag="IWO17_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0291406.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKSLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            1604..1945
                     /locus_tag="IWO17_002553"
     CDS             1604..1945
                     /locus_tag="IWO17_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291407.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            1975..2334
                     /locus_tag="IWO17_002554"
     CDS             1975..2334
                     /locus_tag="IWO17_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ0291408.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            2538..2717
                     /locus_tag="IWO17_002555"
                     /pseudo
     CDS             2538..2717
                     /locus_tag="IWO17_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            2859..4940
                     /gene="cadA"
                     /locus_tag="IWO17_002556"
     CDS             2859..4940
                     /gene="cadA"
                     /locus_tag="IWO17_002556"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0291409.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            5251..6717
                     /locus_tag="IWO17_002557"
     CDS             5251..6717
                     /locus_tag="IWO17_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0291410.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2231 a         1094 c         1438 g         1971 t
ORIGIN      
        1 ggaagaggtt gtgaaatcag tgccttgacc tgagtattag agaaacaaaa cggaaaaata
       61 gcttctcata ttttaccgtt tttttagcta atatcgaggg tcagctgatt gaacaccgtt
      121 tatatcaaga ggttgtgcgc ctgccgttca gctccgagtc acaaggagta atttcaaaaa
      181 acagtttttc atgtttgatg actattgtga cttgtgaccg aggagttggc cggagagcaa
      241 cgtttattta aaaaaaagaa ctgcgaactc tcgcagttct ttttttaaaa tacgggataa
      301 aggaagtttc cagcagacaa gagaagaaag caaaagaaat tgctatttga ttcacaaggt
      361 tctataacga aatcgcaatc aatcaaacat atacaaagaa ggttattcaa atggaaatct
      421 gatttgatga tgcttctgct gttgaaataa agcagcatct agtacctgaa aacaaactaa
      481 tagtaacatt cgaagatgga gtaggtccgt attttcagca tgcaatgatc catatgtaag
      541 tttaattcat ccttgaaatc atcggaccag ataaagttat gaccaatcta tcccaagcaa
      601 tctagaagag atattagtaa aagaacattt actggaagtc ttagatgagc ctataaatat
      661 atgttctgat ggcgtcatca gtggtctgat tgacggaagc ttaggtgtga tcgatttctc
      721 tggaccaaac aacgaaggaa tcaaggggaa tccggcacgt taagaaaaaa agtataaaaa
      781 actagacact tgatgagcgt tcattcaatt gattgaaggg actaggctgg tgtcattttt
      841 tatacatata ttttctcttt cgtcttgata gggcttatgg gataatataa ggaagaaaat
      901 catcgtttta ttcaaaaagg agtttttgac aaatgaaaaa acgtatatta tttatcggtg
      961 acagcattac agacgcagga agagaccgag cagaagcaac tgatctaggt aaaggttatc
     1021 cacttttgat tgccggaaaa ctaatctccc aatatccaac gatggaattt gaatgcatga
     1081 accgtgggat aggaggagac aaaattgcag atctgcaagt acgctggcaa gaagattgtt
     1141 tagctttaca acctgactat gtatcgattt tgatcggaat caatgatacc tggcataata
     1201 ctagcgatca ggctatattc ggaactgaga aatctgcgca gcaatttgaa tccgtttatc
     1261 gaaaatcact aaatgaactt caaacagcgg aaatctgcaa catcatcttg atggagccgt
     1321 ttgtacttcc tataaccgac gaccgtcatg gctggcgtaa agacttagac cctaaaatcc
     1381 aaattgttcg taaacttgct aaggaatatg gcgcattgct ggttccttta gatggcatat
     1441 tcaatgcttt agggattgct tatggttttt cgaaatatac aagagaagat ggggtccacc
     1501 caacgattgg cggacatgag atcattgcac aaagctggat agatgcaatt gccgcagtcg
     1561 atttttttag tggagataaa aatgagcgaa agaaaagtga gaaatgatgg aaatacggtg
     1621 ggcagtaaaa gaagatttac ctcaaatcaa tcagctgatt actgatcatc cagaatcatt
     1681 cgtaaaagca aaaacggcag ctgacctaaa tgataagaat aaactattgc tcgttgctgt
     1741 aacaaaagaa aaacggatta tcggatttgc gcaagcggac gtgcaacagt cagaagaacg
     1801 ggtggatcag cgaccatcag gaaaactaat ccaacacaca ttagtaacaa aagaaaatac
     1861 ggaagaaatc caaaaaaaat ttgcagaata cttgatgctg gaagctaaaa aaagaagaat
     1921 aaaatcaatt tttgcccagt tttaatcaaa aacagtaata gaaggagaaa tacaatgagt
     1981 tgtttaggaa atgtcatctg gtttatattc ggcggactaa tcggtggtct tagctggtta
     2041 gcagcgggga tactttggtg tatcacgatc ataggtatcc caatcggtat gcaatgtttt
     2101 aagatcgcct ccctttcttt tgcgcctttt ggcaaagaag taatttatca ccagtctggc
     2161 atgtcattga tcgttaatat catctggctg atcgtttcag gcctaccaat tgctctcgca
     2221 catgtcgtca gcggcatcct tctatgtatc acgatcatag ggataccttt tgccaagcaa
     2281 tctttcaaac ttgctcgatt ggcgttgatg ccattcggtg cagaaattgt ataaaaagag
     2341 aataataaag gttagaaaac gcatagagtc aggtgggata catgtatttc acctgactct
     2401 atgtgttttc ctgtataagt tctcccatgt gacttttacg tcttttattt atatgggatt
     2461 actgtttatg gaaagttgaa ataattttca ttaaagaact ctttctatat ttttgaaagc
     2521 gtagtggagc ttcgaatatc tgggaaggac attttgcagg gattgaaaaa agatattgtc
     2581 ctatcgaaaa attaatgaat ttagacaaat tgccagcaac aggcttcaaa cttagttgct
     2641 atcctgtcaa gatcaagaag gcaagtgcgg gttggataag agcaggggca atgatcgaag
     2701 agaaaaagaa agaataagcc aaaacatctg tatggaagaa attttccgat gcagatgttt
     2761 ttcatttttt ctttcggata ccttgacata tgaataacca ttcatttata attagttatg
     2821 aagagatatt catatgttga ggcgaggagg tcattaaaat gaaaagttac agattagaag
     2881 gtttagattg tgcgaactgt gcaatgaaaa tcgaaaaagg agttcagaag atcaatggag
     2941 tcaaggaagc aacggtaaac ttcacttctg gaaaactaac gattgacgca gaggaagatc
     3001 atttggctac gattgaacaa gaaacaaaaa aagtagtgaa ggaattagaa ccagatgtca
     3061 aagtaacaga aatagataag gaaaaagtat ctgaacatgg aaatgaaaaa gagagaaata
     3121 cgctgttccg gattttattt tcccttgcag gtatcgcttt attgctatta tttgatttta
     3181 atgaaccaat tcgcttgata gggtatttat tgatttatct gctgatagga tatgatgtag
     3241 tgaagaaggc agtgatgaat atcgtcaaag gaaaaatctt tgatgagaat tttcttatgt
     3301 ctgttgctac gattggcgct atgttgatcg gtgaatatcc agaagccgta gcagtgatgc
     3361 tattttatca aatcggcgag tatttccaag gattggcagt cagtcattca agaaaatcga
     3421 ttcgcgaatt gatggcgatc cgtccagaaa cagctcacgt gcagacgacg gaaggattga
     3481 tgaccgtgaa ccctgaagac gtgcagatcg gtcagtttgt acttgtgaag cctggtgaac
     3541 gtgtaccgtt agatggaacg atcatcgaag gagaatcatt agtcgatacg tcagcactta
     3601 caggagaatc agtccctaaa tctgtatatg ttggtgaaac agtattaagc ggatttatca
     3661 ataaaaataa accattttta gttcaagtag aaaaatctta tgaaaattct actatcagca
     3721 aattattaga attagtagaa aatgccagca gtaaaaaagc accagctgaa aatttcatta
     3781 ctaaattcgc gcgctattac acaccagtcg ttgtaggatt ggccgtttta ttagctgttt
     3841 tacctccact tgttgtttct ggagcggcgt tttctgaatg gatctatcga gcattgacat
     3901 ttttagtgat ctcatgtcca tgtgcattag tgatttccgt gccacttagt ttctttggcg
     3961 gtatcggagg cgcaagtaaa atcggtgtat tagtaaaagg ttcaaattac ttggagttgc
     4021 ttgcgcaaac cgagacagtg gtattcgata aaaccggcac attaacgaaa ggtgactttt
     4081 cgatccagac gatcgataca aaaatcgatc caaagaaatt catgcaatat attgcgagtg
     4141 cagaacagtt ttctacacat ccgattgctc aatctgtttt agaaggctac gcaggtccgc
     4201 ttttaccaac aactaatatc caggaatttg caggtgaagg gatcttagct gaagttgatg
     4261 gcaaacaagt attagcaggt aatcacaaat tgatggaaag atttgaaatc tcttttcctt
     4321 taagtcaaga aatcggtaca ttgctttatc tagcgatcga tcaaagttac agtggttatc
     4381 ttgttattgc agataccttg aaagaagatg ccgtagacgc attggttcaa ttaaaacagg
     4441 ctggtgtgaa gaacacagtc atgttgacgg gcgactcgaa aaaaattgct gatcatatcg
     4501 ggaagcaagt aggcgtggac aagatatata gcgaattgct tcctgaagat aaagtgcagc
     4561 ggttagagga aatcttacaa agaaacaata aaaagaccgc atttgtcgga gatggaatca
     4621 acgatgcacc ggtgctagca agagcagatg taggaatcgc aatgggtgga ttgggaagcg
     4681 atgctgcaat tgaagcagca gatgtagtca tcatgaatga tcaaccatcg aagattgcag
     4741 aagcaatcca tttagcaaag aaaacattga aaatcgtcaa acaaaacatt gtctttgcta
     4801 ttgggataaa aattttggta cttgccttag gtgcttttgg ttttgcatca atgggagatg
     4861 cagtatttgc agatgttggg gtgacagtat tagctgtttt gaatgccatg cgaagcttgc
     4921 atgtaaaaaa taaacgttga tcaatgaaga ggacgaatga acgtcctctt ttttttatat
     4981 ccttttctta atgaaaaatg taaagaaaat agataggatt ataaaataac taatgttttt
     5041 aattttgcag aaaattcaaa atagcttgaa attcaataag gtcaatgaaa taaaagaaaa
     5101 taacttattt ttttgatgga aaaaatatga aaaaacgatg tgtattaaat aaaagaatac
     5161 acacataaaa taacactgtc ttgtaagttt gttaaaaatt gaatatgatt acatctggat
     5221 aacaaatttt tatcataaag gagagaacga atgaacaaca aacaattacg ctggtttact
     5281 gttgcgttaa ttgcatttaa catggtttgg ggcatgggga atgtagttaa taactatgct
     5341 cagcaaggag tatcagttgt cacctcttgg ctattgattt tggccattta ttttattcca
     5401 tacgcattga tcgttggaca attaggatca gccttcaagg atagtaaagg aggcgtcagt
     5461 tcatgggtcg aaaatacgac atctaataaa cgactggcat actatgccgc ttggacttac
     5521 tgggtcgtcc atattcctta tttggcacaa aagcctcaag caattctgat tgctggtggt
     5581 tgggcagtcc aaggaaatgg aaatatcgta aatacgatgt cagtacaaat ggtagcagtt
     5641 atttcactta ttatatttct tgccttttta tatttatcaa caaaaggact ttctactttg
     5701 aaagtgattg gcggactggc cggaacagcg atgttcgtca tgtcgatctt atttattctt
     5761 ttagctgtaa cggcaccgtt catcaatcca acgatggaat ttgccacacc acatatgaat
     5821 caagtgaaaa catatattcc aacatttgat ttcagttatt tcacaaccgt ttcgatgcta
     5881 gtatttgcag ttggaggggc ggaaaaaata tccccttatg tcaactctac aagaaatcct
     5941 gcaaaggaat ttcctaaagg gatgatcttc ttagcagcaa tggtagggat ttgtgctgta
     6001 ttaggctcag tcgcaatggg tatgctgttt gctagtgata atattcctaa agacttgatg
     6061 gcaaatgggg catacagtgc ttttcaaatt ttaggtaatt actatggtat cggtaactca
     6121 ttgatgatca tttacgcatt gacaaacatg attggacaaa tctccgcatt agccttttca
     6181 attgatgcac cactaaaaat cttgttagca gatgcggatc cagaatttgt accaaactgg
     6241 ctacgtaaac gtaatcgtaa aggtacattg gtgaatggtt atcttctgac aggtatacta
     6301 gtaggaatca ttattttgtt gccgattttt ggtatcaaag atatgaatga attagtaaaa
     6361 tggttgacga acttgaactc agttgtcatg ccaatgcgtt acctatgggt gttcttggca
     6421 tatatgatgt tgaataaagc ttataagaaa ttcaattctg aatataaatt tgttaaaaat
     6481 ccaaaaatcg gttttatctt tggtgcttgg tgctttgcct tcactgcgtt tgcatgtatc
     6541 ctaggaatgg tgccaaaagt agattatact gctgatccta aagcatggtg gttccaatta
     6601 tttacgaata tcatcacacc aatcgtcttg atcttattag gtatgatctt gccagcaatt
     6661 gctcgtcgag acaaaaacaa agaaatcact gaaccagaaa cagtggttca gccttaaaat
     6721 agaaatactt gtta
//
LOCUS       DACECR010000127         6275 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.128, whole genome shotgun sequence.
ACCESSION   DACECR010000127 DACECR010000000
VERSION     DACECR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.128"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..226
                     /locus_tag="IWO17_002558"
                     /pseudo
     CDS             1..226
                     /locus_tag="IWO17_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            352..1323
                     /locus_tag="IWO17_002559"
     CDS             352..1323
                     /locus_tag="IWO17_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0291411.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            1463..2776
                     /locus_tag="IWO17_002560"
     CDS             1463..2776
                     /locus_tag="IWO17_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0291412.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            2819..3685
                     /locus_tag="IWO17_002561"
     CDS             2819..3685
                     /locus_tag="IWO17_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0291413.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            3682..4530
                     /locus_tag="IWO17_002562"
     CDS             3682..4530
                     /locus_tag="IWO17_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0291414.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            4544..6016
                     /locus_tag="IWO17_002563"
     CDS             4544..6016
                     /locus_tag="IWO17_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ0291415.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            6033..>6275
                     /locus_tag="IWO17_002564"
     CDS             6033..>6275
                     /locus_tag="IWO17_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0291416.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1955 a         1079 c         1363 g         1878 t
ORIGIN      
        1 tcttgaaaaa ttaattaaag acttagataa agccattgac gacttagtcg tggtgctacc
       61 ggagtatcaa tgtttaacca gtatcccagg tattggtcct gtttaggctg ctggaatact
      121 cgctgaaatt ggtccaattg aacgtttttc tgatgaaata aaattgacaa agtatgccgg
      181 cctatattgg cgacagagtc aatccggtaa atctgaatac gaaaattttt ttacgaaaag
      241 cttcacgtct aatatctatt ttaaaataaa aatattcctg ctaatttgga atcgtttcct
      301 tcttttggta taataatacc tgaaattctt ttgagaggag ttgttcgtga agtgaaaact
      361 actatgaagg atgttgctgc gttagcagga gttggtgtgg gtacggtatc tagagtaata
      421 aatggagtaa aggtgaaaga ggttaccaaa gaaaaagtaa tggctgcaat tagtgagctt
      481 aattatgagc ctgatgaata tgccagaggt atgaaaacga atagtagcaa tacaatagct
      541 cttattttac ctacgatttg gcatcccttc ttttccgagt ttgcctacta tgtagaggaa
      601 gctctcagcc aaaaaaatta taagcttttt ttgtgtaatg cgaacggcag tgcggaaaaa
      661 gaaaaagaat atatccaaat ggtcaagcag agtaaagtag acggaattat aggtattacc
      721 tattcagaca ttgataaata tgtatcatca aatttaccat ttgtcagtat agaccgccat
      781 ttttcggaac aggtttctta tgtaacggca gataattatt taggtgggca aattgccgct
      841 caagaactac ttactcgtaa ctgtaaaaac ttgtgctatg ttgggggtgt ttcaccttac
      901 cctaacgaaa caaacaatcg taaagctggt ttttacaata tttgtgagga aaataaaata
      961 gctgtgaagg tattggatat gcctgaacca attagcaatt tagaggaaca attgctcgct
     1021 tttttttcgg ataatcctga aattgatagc atttttacca tcaatgattt catggctttg
     1081 gatgtgataa aagcccaaaa aaaactaggg aaggaagcac ctaaggattt tcaaattatt
     1141 ggttttgatg gagtcaagct atcgttgcaa aaagactatt tactatcaac gattgtacag
     1201 ccgatagagg aaatggccaa ggtttcagta gaaacactca tggcgatgat tttaaatgaa
     1261 gaaccgccta agcgtgtggt agtaccggtt ttctttcacg ctggagagac gactaaagag
     1321 taaaaaaccg gtttataacg atttttttga gataggttga cagaaagtga tttcaatgat
     1381 atcttaaaac caattaattg gaaacgttac caattaattg aaaaataaaa cggaaacgtt
     1441 tcaaaaaata ggaggaacat cgatgaaaaa gagaatgtta gcaattgcag caattttagc
     1501 attaacagct ggaggacttg caggatgtgg cagtaaggag aaaagttcag gtagcagtga
     1561 tccaggagaa attaagattt gggttcagtt ttctgatgaa acgcctgaag gaaaagcttg
     1621 ggcagagatt gtgaacaatt ttaatgatga atacgagggt gaatataaag ctgttactga
     1681 gtatattcct cgtagcggaa gcggcggtgg atatgaggat aaagtaaatg ctgcaattac
     1741 aaccaatacc cttcctgatg ttattacgtt agatggaccg aatactgcag cttatgctca
     1801 ttcaggagtc attgctccat tagatgatta tttgaaagat gcggacatgg atgatgtatt
     1861 agacagcatt attcagcaag gaacctacga tggcaaaatg tatgcgtttg gtttctcaga
     1921 gtccagtgtg ggtgtctatt acaataagtc gctgttcaaa gatgctggaa ttacagaaga
     1981 tcaattgccg acgttagaag atccttggac ttggacggaa tttcaagcaa tttgccaaaa
     2041 gctgactgat aaatacaaaa cgcctgcaat tgatatgcaa atggcgtcgg gagacgaaat
     2101 gctgacctac gcatatacac catttatatg gtcgaatggc ggcgatgtcg ttaatgctga
     2161 tggaaccaaa gccgaagact attttaacgg taaaaaatcc gttgaagcgt tggaatttat
     2221 tcaagaattg gttgaaaaag gttacaccac aatcagtcct attgaaaaag gatttgaaac
     2281 tggcaaatat gccatgatgc tgagcggttc ttggacaatg gcagatttag aggaaaacta
     2341 tcaagagatt gattatggta ttttgcctta ccccgtttca gatgcaactg gggagttagt
     2401 ttcaccaacc ggaagctggc agttagcaat gactgagaaa acagagaaaa aggatgcttc
     2461 tgcggccttt attaagtttg caaccagtac agaatctagt cgtattatga gtttagggaa
     2521 cagtgttcta cctatcagaa attcaacagt tgatttaatt aaagatgaag tgtcggaagg
     2581 aatgcaggta ttaattaagc aaaaccaact ttctggacat gcccgcccag tggttgtagc
     2641 ttatccgcaa gtatctagag cattccaaca agcagttcaa gatattagct acttcgagga
     2701 aaacccagat gtacaaaaag tagcagatag tcgcgctgct gaaatgcaac aggcaattga
     2761 tacttcacta aaatagaagt ttctaaaggg ggccaatcaa gtgttaaaaa aaccgacaat
     2821 gaaagacaat ttgataggtt acggctttct agcgccagct ttgctgctgc tgacgatttt
     2881 tctgatcata cccgtaatca tggtaattta ttatgccttt actgattatt atttgttaac
     2941 gccagatgct agacaattta tcggcttaga aaattttaag cgactgttcg ctgatccaat
     3001 ttttgtaaaa agtatttgga acacggctaa atttgttgtt tggattattc cggtccaaat
     3061 aggggcagct ttaggaatgg cacttattat caacaaaaat cgtaaaggaa atatcttttt
     3121 taaagtagcc ttttttgcgc cagtcgtgat gtccttagtc gttatttcga ttttatggtt
     3181 gtacttgtta aatcctaatg aaggtctggt taatacgcta tttgcaaaga ttggaattgc
     3241 ttcgcagcct tttttgacta gtcccaaaca agcaatgtat acgcttgttt tcgtatcggc
     3301 atggcaaggt gcaggatatc agatgctgct gtttttaggg ggaatgcaaa atattccgca
     3361 agacgtttat gaggcagccg aaattgatgg tttttctaag tttcagcaat ttcgctatat
     3421 caccatgcca ttattaaagc ccacggcgat ttttatttta cttaccacat tgatttcggc
     3481 ctttaaacta atcattcagc caatggtaat gactcaaggt ggaccaatga attctactat
     3541 gacaatggtt tattacattt accaaactgg atttacagac cgtatggtag gctactccag
     3601 ctccattgcg ttaatcttta ccactatgat tggtttaatt acaattgctc aaagaaaatt
     3661 ggtaaaggag gatgatccag catgaccaag aaatttggac cgctaatgat cgtagaatat
     3721 tgtttgctgt tccttttagc ggcattattc attttcccga tgctgtggat ggtagtttcc
     3781 tctatgaagc ctgaagcaga tgtctatcaa aatttagctt ccttcaaagc ttttttacct
     3841 agtttgaatc cagctaattg gtttaaaact tatcaagagg ttatttcccg gttcagtgtg
     3901 ggaacttatt tattaaacag tattttttat ggtttaacct ttgcctgtgg gtcaatcatt
     3961 gtaaactctt tggcgggttt tgcctttgcg aaggttaatt tttcaggcaa gaaaattatc
     4021 tttggagctt tgttagctct gctgattgtc ccgatggaga cggttttaat tccgcaattt
     4081 accatcgtta attcactagg cttagtgaat actcgcttgg cagtgatttt accagcgatg
     4141 gccagcgcgt ttaatatcta tctgtttaga aatttcttta tcgcgattcc agaagaaatc
     4201 attgaatcag cgaaattaga tggtgcaaat attgttaaaa ttttctttca catcatgctg
     4261 ccaatgtcta aaccagccat agccacagta ggtgtcttat cctttatctc cagctggaat
     4321 gattatattt ggccgttaat ggtcttaact gacagcagca aattttcaat gcaagtggca
     4381 attactacga tcaatactac acagccagtt tatatcaatc aagtgatggc tgttttaacc
     4441 attgcaacga ttccactgat tattgtttat atcgttgcac aaaaatatat tctgcaggga
     4501 cttggcggtt ctggaacggg tataaaatag gagtgaatag tgaatgttgc aaaaactttt
     4561 aaaggccaat gagtttattg agaaaaatgc tcaaacagtc aatcaagaat accggaataa
     4621 atttcatttg atggctccga ttggctggat gaatgatcca aatggttttg tttattatca
     4681 agaggagtat cacttgtttt atcaatatta tccttacgac agcatctggg gaccaatgca
     4741 ttggggccat gctaaaagca aggatctaat tcactgggag catttgccag tagcactggc
     4801 accaggtgaa agctatgaca aggaaggctg tttttccggc agtgccatag aaaaggatgg
     4861 caagctgtat ttgatttata caggacacaa tgttgtggaa gggcaagttc gacaagttca
     4921 gtgtttggca gtttcagaag atggcattca ctttgaaaaa tatgccggaa atccaatcat
     4981 cgcagaagat cacttagctt ctgttgcaac gaccgaagat ttccgcgatc ccaaagtgtt
     5041 tcagcgaaaa gaagactatt ttactgtagt cgctactaaa actgcagatg ctcgtggaca
     5101 aattgtgatg ttccattcca aagatttact aacttgggag ttttattccg tattgctgga
     5161 gggaactcca gaacaaggca ttgtatggga atgcccagac ttgtttgaac tagatggcaa
     5221 agaagtcttg attctttctc ctattcaaat gactccacaa ggcaatgctt accaaaatac
     5281 gaactcaact gttgctttca tcggtactgt cgattggcaa acaggcaaat ttctagtaga
     5341 gaattttcat gaaatcgatg gcggtttaga tttttatgca ccccagacct gtgaaaacga
     5401 agcaggccaa agaataatga ttgcttggat gcaaatgtgg cagcgtaaaa tacctacgaa
     5461 tgatctgggc catggttggt caggttccat gaccttgcct agagagctga gggtggctga
     5521 taatcaattg ttgcaacggc cagtttcatc aatttatcaa gtaattgatc cgcaggaaag
     5581 gattgaatgg gcgaagactg aagagaatcg cttcttactg aaggacttag tatatgacca
     5641 gcactattta cgcttggaaa tcaatttaaa acaggcaacg aaactagaac ttcactatgc
     5701 gaagggaccc gaccatacat tactgcttaa ctatgatgtt gaacgggagt gctttactgc
     5761 ttccagggaa cacatgccgt atgatttatc aggagctgaa aaggaagtgt tgaaaaagcg
     5821 cagtgtgacg attcctttgg aaaataattg tctaaccatc gaaattttcc gtgatacttc
     5881 agctattgag ctttttctaa atgatcgtct gactatgagt acaacttttt atgagacaga
     5941 aaaaggacag gattttcttt tgctggcaga cagtgaagta accttttcat tggaaagcgg
     6001 cttaattaat ctatagaagg gagagctcag tgatgattta tggttcaatc gaagcaggcg
     6061 gcacaaaatt tgtgtgtgcc attagcgatg aagaaatgac gatcaaggaa agagtgagct
     6121 ttccgacaac gacacctgag gaaacaatac ctttagtcat tgacttcttc aaacaatacc
     6181 aagcagattt agcagggatt gggattggct catttgggcc tatcgatatc catcgcgatt
     6241 ccgccactta tgggtacatt acctcgaccc cgaaa
//
LOCUS       DACECR010000128         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.129, whole genome shotgun sequence.
ACCESSION   DACECR010000128 DACECR010000000
VERSION     DACECR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.129"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IWO17_002565"
     CDS             725..811
                     /locus_tag="IWO17_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0291417.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IWO17_002566"
     CDS             complement(932..1621)
                     /locus_tag="IWO17_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291418.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="IWO17_002567"
     CDS             complement(1866..2780)
                     /locus_tag="IWO17_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0291419.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IWO17_002568"
     CDS             complement(2762..3142)
                     /locus_tag="IWO17_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0291420.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IWO17_002569"
     CDS             complement(3592..3879)
                     /locus_tag="IWO17_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0291421.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IWO17_002570"
     CDS             complement(3899..4123)
                     /locus_tag="IWO17_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAQ0291422.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IWO17_002571"
     CDS             complement(4763..5296)
                     /locus_tag="IWO17_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0291423.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IWO17_002572"
     CDS             complement(5317..6159)
                     /locus_tag="IWO17_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ0291424.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACECR010000129         6019 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.130, whole genome shotgun sequence.
ACCESSION   DACECR010000129 DACECR010000000
VERSION     DACECR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.130"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..142
                     /locus_tag="IWO17_002573"
     CDS             <1..142
                     /locus_tag="IWO17_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0291425.1"
                     /translation="MEFNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFC
                     LA"
     gene            complement(256..1386)
                     /locus_tag="IWO17_002574"
     CDS             complement(256..1386)
                     /locus_tag="IWO17_002574"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ0291426.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(2035..3237)
                     /locus_tag="IWO17_002575"
     CDS             complement(2035..3237)
                     /locus_tag="IWO17_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0291427.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3297..3500)
                     /locus_tag="IWO17_002576"
     CDS             complement(3297..3500)
                     /locus_tag="IWO17_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0291428.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3642..4874)
                     /locus_tag="IWO17_002577"
                     /pseudo
     CDS             complement(3642..4874)
                     /locus_tag="IWO17_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            5037..5849
                     /locus_tag="IWO17_002578"
     CDS             5037..5849
                     /locus_tag="IWO17_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0291429.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1794 a         1240 c          959 g         2026 t
ORIGIN      
        1 aatggaattc aaccaagtca atcaaggatt catctcatcc gttgcgtcca aaagaaacca
       61 tatccctaga aaatcactaa attaccaaac accattagaa gtttttttga gttacgtaaa
      121 tggaaagttt tgtctcgctt aatttgacaa ataatacaaa taaataatat aaaaggtaaa
      181 taaaaaagag tggatttctc cactccatga aaagccgact ttttgggacg cttttttttg
      241 ccaagcctca catttttata gccaaacttc ttttgcgact tgctgcaata attggatttt
      301 cttccattgt tcttcttctg ttaaatggtt tccttcttct gttgaagcaa acccacattg
      361 aggactgata cttagatttt ccaatggtac aaattgttgt gcttctttta ttcgttcttt
      421 tattttctcc gtatcttcca aatctggaaa cttagaagta actaatccta aaacaacttt
      481 actttctttg ttactccaat gtcttaaagg agtaaaatct cctgctcggt cagaatcgta
      541 ttccaaaaag aaaccatcat aatttgttga aaacaactct tctgcaatgg gttcatatcc
      601 accggaaaac agccaagaag aagcgtgatt cccgcggcag atatgtgttg tgatcgtcaa
      661 atcttctggt ttgtcttcca gtgcgttatt gataatctct gtacaaatat gggccatttc
      721 tttcggatca attccttttt gaagaagtgc ttctctttta gtttgatcag ctagaaacgc
      781 ccaatttgta tcatcgaatt gcaggtaacg gcaacctttt tcataaaaag catgaatcgc
      841 tgctttgtaa gtagaggaca agtcctcata aaaatcagat agatcaggat aaatctcctt
      901 gatttttgaa gaaccatcat tagataggat ctcttgtcgt aaaatcatcg tgggactagg
      961 gatcgttgct tttgcaacaa aagtatcatc tgtttccact acttctttca gaaaagcaaa
     1021 atcttttaaa aatgggtgat ttggattgaa agataattta tctactaccc gaatattata
     1081 agctggtgct gcttttccgt ggaatgtttg attatatcca gtttctggaa catagccttc
     1141 gataccattt agttcttcta aaaaatcaag atgccaaaat ccacgccgaa attcaccatc
     1201 tgtaattcct ttgatgccga ctgctttttg gtcttccact aattttttta tttcctggtc
     1261 ttcgattgca cgaagttctt cttttgaaag taagccttct tgaacttttt ttcttgcttc
     1321 tttgatcgat tgcgggcgta agaaacttcc gacatgatct actgtaaatg gtctattttt
     1381 gctcatttca ctcactcttt tctttgtttt ttgtaaaaaa aaatcctttt actagcaaat
     1441 actagtaaaa ggacgattat actcgtggta ccaccttttt tcagaaaaaa cattctttct
     1501 ctcagcctga caataaaatc agtggtcttg gtaacgaaag actgttcgtt tttgcctacc
     1561 tttcgacaaa aaatcctcta aggccatttt cgacagtcaa cttctagctt tttacaccaa
     1621 ccaaagcctc tcttgaacca ttttctgtct actttccttc tcatcggata ctattagaat
     1681 aaaattagaa tagcatgttt gaaagaaaag tcaataggga ataagaacaa acaaaaacta
     1741 atttgttatt cttagtaatt tgtttgtgga atacaataaa gcttttctaa tatgataatt
     1801 gttcagtcgt cctttcatct tagatcactt ccttaaaatg atcatacccg tttcaaccac
     1861 ataaaaaagg acttaatttt gaaaaatttc ccatcaaaac taagtccttt ttattatagt
     1921 tatgcaaatt tgcttatttt ctcgctattt ccgatacatc ttgaacagga gcaattcgac
     1981 tgaaaacact ggtgcaaaag catttatttg tcttatggta gaaccgtgga aaactcagac
     2041 tggtaataaa ttgtcatttt ttgaaagaat agcgccctct tgaacattat caatcgcact
     2101 taccacttgt tgatctgact ttgcatataa gtgaccgtaa gttcctaaag tgatttgaat
     2161 attcgcatgg cccaatcgtt cttgaataga tagcggttga atattctgct caattagaaa
     2221 tgcaacatga gaatgtcgta gcgcgtgaag cttaatcggt ttgacacccg cttttttagc
     2281 agcttctttt aaccagttcg caaaagaata aggtggcgta aatgttccgt ccaactgagc
     2341 aacatattca aaatttccaa tacgtttttg caagtcatac cactgtctga gttttaagga
     2401 aagcttcttc cccaacccaa tcgttcgata agcacattta gttttgggag tcgaaaagta
     2461 ccaatcatct tttgtattat aaatcaatgt cttctcaatc gttatcgtgt ttttctcaaa
     2521 atctacattc gaccaagaca aagcttcaag ttctccaatc cgaatgccag tataaaagag
     2581 catttcatac acaatcttcc gatggtgggt ttgaatctta tcatccttca attcattcat
     2641 cactttttga aactcactgt gttcccaaaa ctcaactttc ttattctcag aaggaagcga
     2701 agaaacatct tcaacgggat ttttagcaat caccccctct ttaacagccc gatccaatac
     2761 aatttttagg tgccctcgaa tccgattgat ataagatttt gataacgttt cgccattaac
     2821 ttttttccgt gaattacgtg actctgtctg gcttagttca aataaccaat cctcaatatc
     2881 gtgacttgta atatctcgaa cacgaaaatg atagaaaaac tgcatacgct tttcgaaaat
     2941 gaattgcgca ttctcatatg tttgactttt gacctgtctc tcgtaccatt ttacaaacct
     3001 atccttgtaa aactcttcga aagtcatttg attggtagaa cacagttgat taggaattgt
     3061 cttgttaaaa tcatgtgttt ccatgtactt gtcataagca attttggcat ctttttgtga
     3121 agcataacct cgtttagtct tctgaacctt tcttccattt tcatctatac ctagatacgc
     3181 tcggaagaac catttttttg tctttttatc ttggtaaata tttttaattt ttggcatcgt
     3241 tccattcctc cctgtacttg agaaaagcta ctttaatagt accgcgcttg tactgtttat
     3301 tccgcaactt gaatgtgaaa aagatgttca attactcggg atggcaccac accaacttgg
     3361 cgattattat aaaaatcaat accttcaatc cttgcaagat atgccttgct ctctttaatc
     3421 attcgtgcag cttgactagg tttaaagccc atctctatca aaattgcacg atttaccatg
     3481 aaatcacatt ttgtttccat tctctcacct cctgattctt tactcctttt aaaataatcc
     3541 tctttataat ctattgtttc tcattcgatg gttgtgttat actcgtcttg tcgaggcgga
     3601 gtacgttgca caacttgttg tgtaagcagt actttgcttt tttagtagct tgttttctca
     3661 tttgatatac tttgtaattt tcgttcataa tactcaacct cctcctgata atactccggg
     3721 tctgccttca tattttggag aagtttgatc tgtcttggat tcatttctcc ttgttcaata
     3781 atcaaaggca acaaggaaga atgaaacgtt tgatcgacct gtgcaataaa aactaatgat
     3841 ggtgaaacac catgaattaa ccaactcata gttttctcaa aactttgagg aattgtatct
     3901 aaagaaatgg tgattgcgcc ataattgcta ataaaggctg cccatttggc atcaactttt
     3961 gcacctagat catttggatc acggtcataa aaacaaattg ctccattaag taactcaaag
     4021 aacagtttgg ttaaatcatg ggtttttgaa ataatctttg ccaagctttg cgctttctct
     4081 tttcgatacc gtagttcaaa gcgattcttt acttttgcct cttctaaagg aattcctcgt
     4141 cgcttccgtt gttcatggtc cttctgataa aagcaaaaac gacactgact cttctttgag
     4201 cctaaataca aggtctcacc atttgattca ttgtttatga tatccgtacc gccatgagat
     4261 tcactcactt gaaaagtggt ccacacatgg cctagtatta ccttatgctt aagttctgga
     4321 atgctgagac tccccacaaa atcatctaaa gtaaaatcaa tccgtgtaaa gttcccctga
     4381 taatctaata ccttacgaaa aaactgttgc caatttgttt ttcgtgtttt tagccatctt
     4441 gctaagtgtt tacagccttg acctgaaaac tccatcacag ttcctttaac cgcatcgtca
     4501 atggaatacc gaatcgtgat tacttgattc cacacgtatt ggccaatata gccctttggc
     4561 gcataatcca gttcctcaaa acgttcaatg ggcatttcaa taatctcttc aattagcttt
     4621 aaatgttgat tcgagaaaaa gtgaatggtt agataatcaa tcaaaacacg gttaactgga
     4681 acgacctctt cttgcaaact atccaacaca agattcaatt tttcttcact taattgtctc
     4741 gttccttttt ctactttcga taataactat ctcgaaatcc ccactttttc tgctaaatat
     4801 cgttgtgtcc accccctttc ttctcgagaa actcgaatgg cttcaccgag atcatgttga
     4861 ttaacttggt tcatcgacaa acctccttct gctatttact tgtcaaagaa cctattgaac
     4921 ttattttaag tacattttta ttgtacgcct gtatttattt taagtcaata tgttttgact
     4981 ttttttaagt acacacgtat actaaattta agaacttatc ctgaggagga gttttcatgg
     5041 agatagacaa gcaagcagtc ggcaatcgaa ttcggcagat tcgccaagaa ttaaagttga
     5101 gtatggagaa atttggcaaa ctaattggtg atttaccgcg aagtacagtc aataactggg
     5161 aacgtgggat caaccttcca aaaaccgaaa cccttcacca aatcgcagag ataggtcatg
     5221 tcaccaatga atatcttttg tatggtgatc aagagaatca atacatttta gaaatgctcc
     5281 aaaaaaaggc aggtaagctt gatcctaaga ttgaaggtct catagtagac gaaatcaaac
     5341 acgctgacct tgttagtgaa aaagaaatgg atcgaatgat tgaatttttt attacaaatc
     5401 tcattccacc aactgaacaa gaccagttta ctttccaatg tatcgatgaa aaaaagcagc
     5461 tttatcttgg ttcaactaat tttggtaagg aagcccagct ctatttgcaa catgataatc
     5521 aaaatcatat tctgcacatg atgcccttta ccttctccaa ctttagtgtg gatcgcatac
     5581 tcgtctattt agcaaatcaa aaatcctatt cctattttgg gaaacattta cccaaggaac
     5641 taggtgagaa agccatcctt ctatactcaa ttaatcaatt gaccagcgat gtgcgaattg
     5701 ccccgttggt ctattcagaa gaaaccgctt cttatcagta cacagaggac aatcaatatc
     5761 tactagaaca gcaatatctt tatctaccat tcgtgatgga agtcgagaaa gaacgcctgt
     5821 taaatgcagc gtatccatca tcaaaatgag gtgacaaaat tgataagcac gtgacaaaga
     5881 aaatactgct aggacactcc ccaatgggta gttgtcacag tttcctgttc tttgttagcc
     5941 ccctgttaga accgggggct ttattcctgc agtccggcta cgccttcctg caatagacaa
     6001 aactccagat cgggtaatt
//
LOCUS       DACECR010000130         5936 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.131, whole genome shotgun sequence.
ACCESSION   DACECR010000130 DACECR010000000
VERSION     DACECR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.131"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..94
                     /locus_tag="IWO17_002579"
     CDS             <1..94
                     /locus_tag="IWO17_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ0291430.1"
                     /translation="VLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            106..576
                     /locus_tag="IWO17_002580"
     CDS             106..576
                     /locus_tag="IWO17_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0291431.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            593..1372
                     /locus_tag="IWO17_002581"
     CDS             593..1372
                     /locus_tag="IWO17_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0291432.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            1359..2180
                     /locus_tag="IWO17_002582"
     CDS             1359..2180
                     /locus_tag="IWO17_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0291433.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            2197..3246
                     /locus_tag="IWO17_002583"
     CDS             2197..3246
                     /locus_tag="IWO17_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0291434.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            3259..4263
                     /locus_tag="IWO17_002584"
     CDS             3259..4263
                     /locus_tag="IWO17_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0291435.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            4896..5915
                     /locus_tag="IWO17_002585"
     CDS             4896..5915
                     /locus_tag="IWO17_002585"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0291436.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         1961 a          972 c         1296 g         1707 t
ORIGIN      
        1 tgttttgatc cagcaggtgc taactgattc aaaaaaaatg atccagtttt gtaacgagcg
       61 gctcaatcag aaaatagaag aagacgaatt ttaggaggaa gaaaaatgat tgtattgaca
      121 agagtcgacc atcggttatt acacggacaa gtagcttttt catggacaca aacaataggc
      181 gcagactgta ttttgattgc taatgatgat gtacctacga atgagatacg aaagacaacg
      241 atcaaattag ccaagccaca aggagtcaaa ctagtgatca aatcaattga tgactcgatt
      301 gccgcgttga aaagcggggt gacagataaa tataagttat tcatcgtagt ggaatcgatc
      361 gaggatgcat ataagctggc agaagcgtat ccagaaatca aaaccatcaa cttaggaggg
      421 atcaaggcaa aacctggtac tcgcagcatc ggaaaagcgg taaatatttt accagaagag
      481 gaggaacgct tgaaacgatt aattgacaaa caaatagaag tagaaatccg tcaagtacca
      541 gcagataaaa aagtggatgt aagagaagtt ttgtaagaaa ggaagaacct caatgattgt
      601 acaagcagtc ttattaggaa ttgttgcatt tgtcgcacaa tcagaatatg cactagggac
      661 atcgttgctt tctcgtccga ttgtgacggg gctgtttaca ggaatcgtgt taggtgatgt
      721 aaaaactggg atcattatgg gcgcaacttt agagttagca tttatcggat cgttctcagt
      781 aggtgcttcg attccacctg atgttgtgac cggtgggatt ttagggacgg cttttgctat
      841 tacagctgga gccggtacag aaaccgcgct tttactaggt ttgccgattg ctacgttgac
      901 attgatattg aaaaatattt acttagggct tttgattcca attatgaacc ataaagcaga
      961 caattatgca gaagaaggaa attataaagg agtcgagcat atgcacttgc ttgccggtat
     1021 cggtctttcc ttgatgcttg gtgttgtggt aatggtttct tatatggtgg gaagcaatac
     1081 gatcggaaat ctattaaata tgattcctga cttcgttcaa aaggggttgt cagtagctac
     1141 agggttgatt ccagctcttg gttttgcgat gcttgctcgt ttgctgatca acaaacaagt
     1201 tgcaccatat tttttcttag gttttgctat agcttcttac ttagagattc ctgtgaccgg
     1261 agtagctatt tttggtgcga ttttagcagt tgtggtagtc aatattatga atgtccgtca
     1321 actaccactt caaacaactg caggggaggt agaagaagat gaagaattct gagatcaaaa
     1381 acagcaaaat aacgaaaaaa gagctaaatc acgtattttg gcgttctttt caaatggagt
     1441 tttcttggaa ttatgaacgt cagatgaata tggcttatgt ttatgcaatg attccgattt
     1501 tgaaaaagtt atatcagtcg aaagaagaaa tggcagcagc tttgaaacgg catctagagt
     1561 tttttaacac gaccccacat atcgttacac tgattttagg aatcacggct gcaatggagg
     1621 aagaaaataa ggatgatcct gaatttgatg tgactgcgat cgatagtatc aaaacatctt
     1681 tgatgggacc tcttgcaggg ttaggagatt ctttcttttg gggaactttg cgattgattg
     1741 ccacaggtgt gggtacctct ttagccatgc aaggaaatat tttaggccct atcttgttca
     1801 ttttgatttt caatattccg cacattctgt ttcgctatct agcaacaggt tggggatatc
     1861 gcttaggaac gggttttttg aaaaagatcc aggcaaacgg tatgatggaa agtttgacat
     1921 taggcgcgtc tattattgga ctgatggtcg ttggagcaat gactgccacg atgatcacca
     1981 tcaatattcc tttaaaaatc ggttcaggag aaaatgctgt gactgttcaa agtatttttg
     2041 atgatatcgt tccaaatatc ttatgtcttg gagcatttgg catagtgttt tatctattga
     2101 agaaagaagt gaaaccatta acgatcttga tcggactagc tttctttgga atttttggtt
     2161 cgtggatagg gattttttaa aaaaggagca gagaaaatgt ttacgatgca agattatatc
     2221 tatgaagaaa aggaagtact aagtacgatc ttgaaaaaga atgatttttc gacaagagaa
     2281 catatgaaaa aaacagtgaa tttactgatc ttagcaacgg gttcatctta taacgcttgt
     2341 ttagcagcga agccagcatt ggaatcttat ggtgatctta ctgtcgatat tcaagaacct
     2401 ttttattttt tcaattatgg aaaactttct ccttcaatcg atactgtgat tgctgtgtca
     2461 caaagcggga aaagtgcttc tactgttgaa gcagtaaaaa tgatacaaaa acaagggtta
     2521 cctgttgttg ctatcacaaa tgacgtgcaa agtccacttg ctttagaagc tgcacaaatt
     2581 attgatttag gtgccggggt agaatcagta ggcttcgtta caaaaggcta ttctgcgact
     2641 gttttacagt tactactctt agggatagga attggaataa gtaaaaataa aatcagcaaa
     2701 aaaatagaac aagactacat gcaacagttg cgcaaaataa tcaaccattt accagcgatt
     2761 attcaaaaaa cggaagaatt ttttgatgga tatcaaagtt tatttcgttt agcacagcgt
     2821 ttcgtcacca ttggatatgg tccaaactgg ggaacagcta aagaagcaga gacgaaattg
     2881 actgaaacaa tccgcgttcc gtcacaagga tttgagttag aagcttatat gcacggccca
     2941 tacctggagg cagatgcttc gcatttgcta ttctttattg aaggggaaag tgtaaataag
     3001 gaacgttcgc aaaagttgca aagatatatg agtcggtacg ttggagagac tctcaccatt
     3061 accacaaaaa aagcaagaaa cgagaaaaca cttggattag caatagaatg tgatgaatat
     3121 ctgtctgtat tagcgcttgt cgttccgttt caactattcg cttataaaac cgcagtagct
     3181 aaaggtattg acttgaacaa aaagattttt gaagactttg atacagtctt gaaaagtaaa
     3241 ttataagagg tggaaatcat gctgaaattc aatgaagaag aacaaatcaa agcaataaaa
     3301 ggcgcactag ctttacgtcc gcaagtagaa gaaatcatcg ataaacttta tgcggaaaaa
     3361 tttgacgctg tctattatct aggaatcggt ggaacgtatg cttcctcgat gcaagctgtc
     3421 acttatatga atgggaaaag caacttgcct gtctttgtac agcatgctgc agaatattac
     3481 actactggaa acaaaagatt aacaaaagat tctatcgtta ttttgtcctc tgttaccggt
     3541 acaactcaag aagtagtgaa agcagtagaa caaatcaaga aagtaggagc gacactgatt
     3601 ggatttatcg ataaagcgaa cagtaagttg tctcagctat gcgattttgt ggtcacttat
     3661 ccagctcctg gaacagaaca aatcaaattt tttatggcag cagatcgatt gatgtatttg
     3721 aatggagaat tcgaagctta ttcagaatac tatgaacagc tcgaacgtta tttaccaaca
     3781 gggttagtgg aagcagagaa aaaagcagat gcatttggct tatcatttgc agaaaaacat
     3841 cgacgtgata gtatgcatta ttttattgga gcagggaatc aatggggagc tgtttattca
     3901 tatgccatgt gttactggga agaacaaagc tggcttccat cgaaatctat ccatgctgcc
     3961 gagtttcttc acgggacgtt agagatcgta gaagaaacaa ccccagtcac attattttta
     4021 ggcgaagatg aacagagagt gttatctgaa cgtgtggcta aacttttgcc aaaaatttgc
     4081 agcaattata cattgataga cacaaaagac tatccagtag aaggtatcag tgaaaaatat
     4141 cgcggacgtg ttttgtcttt cttattgatg catgtagtga cacaacgcat cgatgctcat
     4201 gtggagcagt tgaactgcca tccattagag atacgccgct actatcgtca gtttgattat
     4261 taggatagac tggataaaaa gaatataaaa aataaagaga gcagcgataa ctgctccctt
     4321 tattttttta aaatattggc atcaattacg ttataaaaca cattttttgc attatctaca
     4381 gttcaactta taatctgttt aatgggacat gtgggtgcca accatttttt gttatgcaat
     4441 agtactattt agttgttgca tggatccgta gtaaaagtcc aagtacaacg agttgcgcca
     4501 gtttacatag cgttttgtat ccagaatgtt gactaaaaca aaagttccta tgtcaaccgt
     4561 caacagagac aatctgtccg ttgcaggacc tgacagattt agaaatgtcc aatctgtctc
     4621 ttatgtattt taaatcaata ctcactagga gaagtttcct aacctcctaa tagtaatgct
     4681 ggtagtaaca ttgtcaaaac tgcttcctct taggaacaag caaagcataa cgagtgtaaa
     4741 aataaaatgt agactattgt aacatatcat tgattatttc aagtaaaaac gataaaagaa
     4801 gtaaacaaac acttaaaaac aatggaaatt agtcccaact ttaagaaatg tatactataa
     4861 tggaaattat caattattta tttggaggaa tatccatgaa acaaaaaatc gcagttcttg
     4921 gtcctggatc ttggggcact gctttagcac aaacattggc agaaaacggg catgatgtcc
     4981 gtatttgggg aaatgtacct gaacagattg atgaaatcaa taagtatcat acaaatcagc
     5041 attttttacc tgatttgacg atcccagaat cgattatcgg atataaagaa ttggcagaag
     5101 cagtggataa cgcagatgct attttatttg ttgttccaac aaaagcgatt cgttcagtcg
     5161 cacaggaact tattggtaaa atgaatacta agccaatcat tatacatgca agtaaaggac
     5221 tagaacaaga tacgcataaa cgaatctcag aagttttggc tgaagaaatt ccagaagaaa
     5281 aaagacaggc aattgttgta ttatctggtc ctagccatgc tgaggaagtg gcagtacatg
     5341 atatcacaac aattacagct gcaagtattg atcaaaaagc agcagcctat gtacaaaaac
     5401 tgttcatgaa tgattatttc aggatttata caaataatga tgtgattggc gtagaaactg
     5461 gtgcagcact gaaaaatatc attgcaattg gtgccggcgc tatccatgga ttaggattcg
     5521 gagatgatgc aaaagcagca atcatgactc gaggattggc agaaatcagc cgtcttggtg
     5581 tcgcgatggg tgccaatcct ctgacattta tcggtttgag tggtgtgggg gacttgatcg
     5641 ttacttgtac aagtgttcat tctcgcaatt ggcgtgccgg taacttgtta ggacagggac
     5701 ataagctgga agaagttcta gaaaacatgg gaatggtcgt tgagggtgtg gcaacgacaa
     5761 aagcagcagt agagctagct caacagctag gtgttgagat gccaattact caaacaatct
     5821 ataatgtttt gtataatgga gaagatatca aaaaagcagc aaaagaaatc atgttacgtg
     5881 acggaaaaat ggagaatgaa ttttctcttc gttaaataga tgatcaatag ttaatc
//
LOCUS       DACECR010000131         5724 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.132, whole genome shotgun sequence.
ACCESSION   DACECR010000131 DACECR010000000
VERSION     DACECR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.132"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            183..638
                     /gene="tnpA"
                     /locus_tag="IWO17_002586"
     CDS             183..638
                     /gene="tnpA"
                     /locus_tag="IWO17_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ0291437.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            903..1001
                     /locus_tag="IWO17_002587"
                     /pseudo
     CDS             903..1001
                     /locus_tag="IWO17_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1224..1454)
                     /locus_tag="IWO17_002588"
     CDS             complement(1224..1454)
                     /locus_tag="IWO17_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ0291438.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1684..2502)
                     /locus_tag="IWO17_002589"
     CDS             complement(1684..2502)
                     /locus_tag="IWO17_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0291439.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            2663..3352
                     /locus_tag="IWO17_002590"
     CDS             2663..3352
                     /locus_tag="IWO17_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0291440.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            3366..4868
                     /locus_tag="IWO17_002591"
     CDS             3366..4868
                     /locus_tag="IWO17_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0291441.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            4881..5357
                     /locus_tag="IWO17_002592"
     CDS             4881..5357
                     /locus_tag="IWO17_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0291442.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(5454..5564)
                     /locus_tag="IWO17_002593"
                     /pseudo
     CDS             complement(5454..5564)
                     /locus_tag="IWO17_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1950 a          837 c         1035 g         1902 t
ORIGIN      
        1 acttaaaagt tttagaccct tttattttct taatgacttc agtcagtttt gtgcgtaaag
       61 cgtgcgaaac aagaaaaccg cgcgtttcta cgcgtggaag aagtatcttt agcgataaaa
      121 actcctttcg atatactagt gagtggctac caaccgctca tcagatcgaa aggagttttt
      181 tcatgtcaaa tgacgataaa agtttagcat atactcgttg gaattgtaag tatcatatta
      241 tttttacacc aaaatatcaa cgaaaagtaa tctatgggca attacgaaaa gatcttggaa
      301 aaatacttag gaagttgtgc gaaatgaaag atgtggaaat catagaagca catgcaatgc
      361 tagatcatat tcacatgctt gttcgagtac caccaaaaat atgtatatcc agttttatgg
      421 ggtatctgaa aggtcgtagt gcggtactaa tccacgagca gcacgcaaat ctcaaatata
      481 aatacggaaa caaatccttt tggtcgaaga gatactatgt gagtacagtc ggattgaatc
      541 aaaagacaaa tgaaaaatac atccgtgaac aagaagctga tgatcgcatt cgatatatta
      601 taagtaaaag agagtacgta gaaccattta aaaaataaag agaagtaagt gagtgaagat
      661 gagcagttgg cggtctttta aaaatcccct tttaaggggc agcgagtgtc gaccccttct
      721 agggggatta aaaatacacc cgtcctcacg ggtgtatggg ttactgatag acttgatttt
      781 aactggtgtc agaaacttat gtccagaaag ggtgatgaaa ttttggcaat ttacggctat
      841 tcaagaatta gcacaagcaa ccaagattac aaaacacaaa ttcaaacatt agaaaatgct
      901 ggcgcagaaa taatcttcct agaaaatcac tggattatcg aacacctttg gaagtatttt
      961 tgagttacgt aagtattgat gatctgtcta acttaatttg acaattaaga gtatatttac
     1021 ttcagatggt aaggcaacaa aataaaatac aataggcaga tgaactaata aataaaatct
     1081 gtattaaata cagataaatt aatataaaaa tcaaaaggat cataaacttt taacaagtga
     1141 tgaaatgggt ggaaattgat tcttatagac agtttgtctc ataacattga agtattttgc
     1201 aattttagca ttgaaacact tttttaatct tcaacgttgt tcttataaac aagatgattg
     1261 ggaatctttt gaattaacac tgtatagctt agaacaccct ttatagaaaa cattcgctta
     1321 gcaatcttaa taattccttg tgcccgatgt cgtttagatt cagtaggttc ctgctcatcg
     1381 atcatggaaa aaatttgaag gatcagatcc ttaataaatt tatttaagag aggatgatcc
     1441 attgcttcat ccatactcga taaactagtg ataatgagct acacgttttt ctttcaagtt
     1501 gatcaatagc ttccacgata aaaatatctt tagataaaat caagaaccgt aggaactaca
     1561 gggatatttg gtcaataaga gccaccgcta ttagtaaaaa ataagctatc aagtatgctc
     1621 tcatcccaag aattgcctac ttcaatcgtt aaaaaccgtt aggtttagct aagtgggggt
     1681 agttcactca atacttttat caataataga gcgcaaaact ttttctctta attcaactga
     1741 atcaatatta ttgttttgtt cataaccatg aactaataga tcacaaatat aaagttgtga
     1801 tatttttcca gctaaagatc caccattaag aaattcttca atggctgttt gtaaaactaa
     1861 gtctgcagac tttcctatag gtgaatgaat atagttagtt atagcaataa tttttgcgcc
     1921 atttttttta gctattttca atgaatcata agtatctttc gttttcccag ataaagaaaa
     1981 aatgattact aaatcattaa ctgttaataa agaagcgact tgggcttgaa aatgagggtc
     2041 tagtacagct tttgcttgaa ctcctacgcg aagaaacata ttttctaaat caagactagt
     2101 atttccacta gatcccactc caaatatata tatgtttttt gattgagtga tcattttaat
     2161 ggcttcattc aatttttctt cgtctaaaag cctaatagtg gaatagagag cttctgttaa
     2221 actattagca atttcatcat agtactttac actagtcgga tgttcttttt tttgactaaa
     2281 gtcttccttt gcaatttcaa tttttaaatc agaaaaacca gaaaaaccaa ttttttgaca
     2341 aaagcgaata atagtagcat cgccaacttt tgtgtttttc ttaatatcac tcatcgtact
     2401 ataaataata gttttcccag aatttaaaat aaaatcagcg actttttttt cagacttcgt
     2461 taaaaatgga taattttctt taattaaata gatataactc atacaaactc ctctacaatt
     2521 tcaacttttc tccattatac tccaaaataa taaaaaaaac tcctaaatac tccattttta
     2581 tctttacttt tatgaaagcg gatgctatat tgtttttgga gttgttatcc aaaaataata
     2641 aaaaattgga gtgtttagga taatggataa aaagaggttt ttagatctta tttcaaaagg
     2701 tttgattgtt tcctgtcaag cattacctgg tgaaccattt tacaaagagg aagaaagttt
     2761 aatgccttat tttgctttgg ctgctcaaag ggctggagca gttggtatac gtgcaaattc
     2821 tgttcgagat attattgaaa ttagaaagtg tgtatcatta ccaatgattg gaattattaa
     2881 aagagattat ccaccagaat ctccattcat tactgccact atgagagaag tagatgaatt
     2941 agtagaaacc ggtgttgagg taatcgctct tgattgcact cttagaaatc gacatgatgg
     3001 actatctata aattctttta ttactaaaat aaaagaaaaa tatcctcatc agttatttat
     3061 ggcagatatt tctacatttg aagaaggcaa aaatgcatat aaagcaggaa tagattttgt
     3121 aggaactact ttaagtggat atactgagga aagtaaaaaa caagatgggc cagatataga
     3181 tttgattaaa aaattggtag aagaagggat ttgtgtaatt gctgagggaa ggattcatac
     3241 tccagaacaa gcaaaagaga taaaaaaaat aggagttgca ggtattgttg tgggtggtgc
     3301 tattactcga ccacaggaaa ttgcatcaag atttgttaaa gcattaagtt agggggagaa
     3361 ataaaatgtt taaaaaattt tcacaattag gacgcgcttt catgttacca attgctatat
     3421 taccagtagc cggattacta ttgggactgg gaggagcgtt aacaaatgag agtgctataa
     3481 atgcttatcc atttttagat caaccgtggt tgcatacaat tttaagtatt atgagttatg
     3541 ctggaaatgc agtttttgca aacctagcac taatttttgc aattggtatt gctgtaggac
     3601 ttgcaaatgg agataaagga acagctggtt tagctggtgg agtagcttat ctagtatata
     3661 ctgcaactat tagtgggttt cttgaacttt tttcagcaaa agatgcaaca ctagatacag
     3721 gtgtagtggg atcaattgtt gttggtggta ctgttgcatt tttacacaat cgttatagaa
     3781 aaattgaatt accacaattc ttaggttttt ttggaggatc aagatttatt ccgattgttt
     3841 ctagtttagc tgcaatcatt attggttctt tcttttattt aatttggcca ccaattcaaa
     3901 atggtttagt agttgctggt gaacaaattg cccaaatggg tagtttagga acttttttat
     3961 atggtttttt attgcgttta acaggtgctg ttggactaca ccacaccatt taccctttgt
     4021 tctggtatac atctttaggt ggtaccgaaa ctgtagctgg atcaacaatt gctggagccc
     4081 aaaatatttt ctttgcccaa ctagctgatc caaaccacac tggtctgttt acttatggta
     4141 ctagattttt tgcaggaaga tttgcaacaa tgatgtttgg attaccagca gcatgttatg
     4201 caatgtatcg tgcaattcct aagaaaaatc gtaaaaagaa tggtgggctt tattttagcg
     4261 gggcccttac atcattttta actggaatta ctgaaccagt tgaatatatg tttttatttg
     4321 ttgctccttg gctttatgtg attcatgcat tcttagatgg attatctttt tattttgctg
     4381 atatcttaaa tattcgtgta gggaattcct tttcaggtgg tttgatcgac tttttattat
     4441 tcggtgtact tcaagggaat gacaaaacta attggttaaa attaattcct tttggtattg
     4501 catgggcatt tatatatttc tttgttttta cattctttat caaaaaattt aaggtagcta
     4561 ttcctggaat ggaagaagaa tcagaagttc attctgaaaa agtaataaac aaaaatacat
     4621 cattacatga agagtctctt atcattattg aagctcttgg tggaaaagaa aatattgagt
     4681 ctgttgaagc ttgtgcaact cgtttgcgtg ttgctgttac gaatggggag attattcaaa
     4741 aacaaacaat aaaagacact ggtgcaactg ctgtcttcga agtgaagggt ggcatccaag
     4801 ctgtttttgg agggaaagct gacttattaa gtcaagaaat caatcaaata ttaggaaaag
     4861 ataattagga ggtttttaat atgtttgaaa ttttcaaaaa gaaaaaacaa gaaacttttt
     4921 atgcaccttg ttccggaaaa atcatttcca tcacagaagt aaatgatcct gtcttttcac
     4981 aaaaattact aggtgatggt tttgccgtca tacctgatgg ttcaaaaatt tatgctccat
     5041 tggctggtcg agtaacaaat atatttccta ctaaacatgc tattaatata aaaagcaact
     5101 caggcattga ttatttgatt catattggaa ttgatacagt tgaattacaa ggagagcctt
     5161 tttcgatttg tgttcaagaa aatgatgagg ttactcctga aacctcgttg gttgatgtgg
     5221 attttgacaa aataataaaa tctggtaaag atacttctgt tatagttgtt tttccggaga
     5281 aaaatcagtt caatgattta ataatagata atggtcgaat tgctcatggg aattcatgtg
     5341 gaaaaatcac tttttaaaaa agtgtatata aaaagctata aaataagcta aataggatat
     5401 ttgtcaaatg aagttggtga acagaaagaa ctgttttctt tctgttcatt tttttatttt
     5461 attagtaatg attccccatg ccaaatcatg atccgtaatt taaagttata aaagcttcga
     5521 aatccattgg catttcgttg gaataccttg atatgattat tcaaatataa aatatagaga
     5581 gaagagagag agggaagata tttataaagg gcaaagttat gtttactcaa cttaggaaat
     5641 agaaatagat caatttatat tctcttgtca atcgaatatg aaccttagca ctatatacca
     5701 aatatagtaa aatgctgaaa gatg
//
LOCUS       DACECR010000132         5681 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.133, whole genome shotgun sequence.
ACCESSION   DACECR010000132 DACECR010000000
VERSION     DACECR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.133"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(27..779)
                     /locus_tag="IWO17_002594"
     CDS             complement(27..779)
                     /locus_tag="IWO17_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0291443.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1143..3809)
                     /locus_tag="IWO17_002595"
     CDS             complement(1143..3809)
                     /locus_tag="IWO17_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0291444.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4150..5601
                     /gene="cls"
                     /locus_tag="IWO17_002596"
     CDS             4150..5601
                     /gene="cls"
                     /locus_tag="IWO17_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0291445.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         1649 a         1193 c          991 g         1848 t
ORIGIN      
        1 tataataaaa tcataaaaag aacccttcac gagtcgtgaa aggttctttt tttatctgcg
       61 gaccaaagga gcagtctata ggtcagctta gtcgtactaa ggatttctcg tttttcactg
      121 aatagtttgc gaatgtacct ggtcacaaca ggatcttttg cgtacgcgtc atagtatagc
      181 ttcatcaatt cttcagtaat tgattcctgt gtaaaataag taaaatcttt tttcttcact
      241 ttgaatcctt catcaattaa gtatgtttca aattgatcca ttacaactgg cgatgtatta
      301 ggatcttctt ctatcattcc tagttgcttt tcgataggat aaaaaacatc atcgattgat
      361 tccactactc tgattatcac gcaccatttg cttgactttt tcatcaattt ttctaattga
      421 ataagatgta ccaatgctgg atttgctgct gtaaaaacca tttcatatgt ctcatttgat
      481 tggataaatt cttctaacga ctgtttgata aaggaaacat ttggcaactc atttttttct
      541 gcttcttttt ttgcattttc tagcattcct gaagaaaaat cgatcaaatc atagcgatta
      601 atttcttttg ctaaggaaag aaatctgcca gatcctccag caacgtctgc tgctgtttca
      661 gcaggcagga ctttctccgt tttcagataa ttttttacat cttcagcaat agttacttgc
      721 gattcttgct ggatttcata atattcttca gcaaattcat cccattcttt ttcattcatt
      781 tgatttcctc taattttttg ttatatgttt tcacttttcg gaataaatca ttctcctctt
      841 ttatctattc tatctctttt gatgaataat aaaatgattg gctttccgtc tatcataaat
      901 aaaaaaaggg actgtgacca aagtcgtgtc aaagtccctc tttccgaata aacagtgtcc
      961 aatcagatga ccctcagtat tagctcaaaa tgctgatttt acaacttctt ccttaaacgg
     1021 tgttctcctg ccaactcttc ggtcacaagt cacaatattc aacaaacatg agaagctgtt
     1081 tgttgaaatt gcaccttgtg actcagaatt aaacggcagg ctcacaacct cgttgattcg
     1141 ttttatttat ttccttgtac tcgtacaatg atcaatttag tgatttccat caaaacaatt
     1201 gtcacaagtg cgattcccat acatgtaaag acttctgtca atccaaatgc tgatgggata
     1261 gagaagactt ctttcagtcc tgggatcatt gttaagcagt aaagcgcgct acatacaact
     1321 acagctagta agacacttct gttagagaag aaccctgctt ggatagctgt ttgtgtgttt
     1381 gaacgtgctg ggaatgtttg caatgtccga caccaaatca atgttgaaaa tgacatggct
     1441 acaccgaatt caggtgaatg ctgcatgcca agccattgag caaagataac agcaatccca
     1501 atcagcacac cacgatacgt tacagaaatc aatgttttgt ccgtaaagat tcctgaattt
     1561 ggatctcttg gcgggcggtt catcgtagca ggttcagctt tttctgtacc taatgcaatt
     1621 gctggcagtg aatcattgac taaattgatg aacaacagtt gtaatgctgt aaatggattg
     1681 gaccagtcag caactaaggc aaagatgatc gcaatgatcg cacctaaatt tcctgcaaat
     1741 agataagaaa tagctttctt gatattatca aacacattac gtcctacttc tactgcgctg
     1801 acaatagaag caaagttatc atccgtcaat accatcgctg ctgcatcttt tgccacatct
     1861 gtacccgttc ccatagcaat tccgatgtct gcttgtttca atgctggtgc gtcatttaca
     1921 ccatcacctg tcatagctga gatatgacct ttattttgcc aagcacgaac gatacgaatt
     1981 ttattctcag gtgaaacacg tgcataaaca gtgacttttt ctaaaacttc gtttagttga
     2041 tcttctgtca attcatctag ttccgtcccg gtcaaagcca ggtttccttc ttcaaaaatc
     2101 ccgatttgtt ttgcgatagc aacagctgtt gttttgtggt caccagtaat catgattgtt
     2161 ttgattcctg cacttcttgc ttctgttacc gcctgagtca cttcagcacg tggaggatcg
     2221 atcatagcaa gcaaaccaac aaggatcaaa tcgtcttcat cttcaaatga taaatcaaaa
     2281 ttatcaagtg gacggtaagc aaaagctagt acacgtaatg cgcgtgttga aaaagcttca
     2341 ttttgctctt ggaatttttt catcaattca tccgtcatcg gaacgacttc accatttagc
     2401 aatacttttt tactgcgttg gaagacgata tctggtccgc ctttaacaaa caagtaattt
     2461 ttgttatcaa ttgtgtgtcc agtagacatt aattttcgat ctgagtcaaa aggaagttct
     2521 gcttgtcttg ggtagttgtt tcggatttcc tgataaggct ggtttaattt ttcactaaag
     2581 tcgataaacg ctacttccgt agggtcgccc aatttagtac cgtcttcact gatcgatgca
     2641 tcattcgcta aaacactgat ttcgatcaag cgacgttcgt cagctgtcca ttttgaaggg
     2701 tcatttgtga aatccccagt atcgccatta cctaaaaagt agtcaacaac tgtcattttg
     2761 ttttgtgtca atgtaccagt cttatctgta cagatgatac ttgtcgaacc aagtgtttca
     2821 actgctggaa gcttccgaat aatggcatga cgttttgcca ttttattcgt tcccaaagat
     2881 aacacgattg ttacgataga ctgtaatgct tctggaatag cagctacggc tacggctacg
     2941 gcaaacatca ctgcgctgac gatatctgca gtcatatctc ctgtaccttc tccaagataa
     3001 atacgaaaca gttggatacc taaaatcaat aaagacaatg ccaaaatagc caaacttagt
     3061 tttttactga atttatccag acctcgttgt aacggagtga cttgctcagt tgttgattcc
     3121 aaaaggccag caacttgtcc gatctccgta ttattcccag ttgaagtcac taagaattta
     3181 ccgcgtccgt aagttacgat cgttccactg aataccatat ttgtacgatc tccgattggt
     3241 accgtacttg tgatatccgt tacttctttt tctgcagaag tcgactctcc tgtcagcata
     3301 ccttcatcta cttttaatga tcccgcatct agtaaacgtc catcagcagg tacataatcg
     3361 cctgcgtcta acaacacgat atctcctacc acaagttcgt ttgccggaat cgttttttcc
     3421 ttaccatcac gaatcactcg agcatcgggc gctgaaaggt ttttcaacgc ttctaaagaa
     3481 ccttctgctt tttttgtttg gacaacactg attatagaat tcaacatcaa tactgcaaaa
     3541 ataactaaga attcgatgta gtcacccatg accatctgaa tcagtgcgac aatcagcaat
     3601 acgataacca ttgcatcttt gaaagtttct aagaacaatt tccaagttgg cttcttcggc
     3661 ttttctttta gctggttcaa gccatctctt tctaattttt ccttcgcttg cgtggtgctc
     3721 aatccatcgg ttgagctctg cgtttgctgc atcaacgagt cgatcgtttc tttatagacc
     3781 gcttgttgtg cttgttcctt gttttccaaa tttcatcccc cattttttga tcttggccct
     3841 agtcctgtct gaaaaaaaag agacctaaga caaatacgca tttgtcttag gtctcaccat
     3901 ttcatacaat accagaaaac catttggtta acttactgtt gatattatcg caacaaacgt
     3961 tgccggttac tcccctaagg agatattcaa ctgtactaat atcctacctt attaacctac
     4021 aaaaagcaac tactttagcc cgatttacct acattttcat gaaaggagtg aaaagtatat
     4081 tcaccttggt aggcttcttt cttctatcat gctatactaa tgttattgta tgtaaggagg
     4141 aacatttttg tggtatctag tattataacc gccctttatc tattaaatgc acttattgct
     4201 ttggtggcta ttttgatcaa accccgagat gtagcagcca tttgggcatg gctcttagta
     4261 ttgtttgccc ttcctggggt gggctttgta ttatatttat ttttcggacg tggattaacg
     4321 gataagaaaa aattctatct ccgacaaagt gacttgaaag aattagaaaa ctttcagtct
     4381 tttagaggag ataccattga acattacgat cctgacatgg gcgataaaga caaacagcaa
     4441 tttgttgact tcttctcttc attaaatcgt atgccgctga caagaatgaa ttctgtcact
     4501 cttctcacag acggacaaga gaaattggat tcactgcttc aagatctaaa aaaagccaaa
     4561 cattcgatcc atatcgaata ttacgcattt gtgacagata atatcggcca gcaagtctta
     4621 catgttttag aagaaaaagc cgcagaaggc gtggaagttc gaatattata tgatgcattt
     4681 ggctctcatg gcacaaaagc aaaagatttc aatcgtctaa tcaaaaatgg tggacatgtc
     4741 catacatttg ttacctcaca aagggcatta cttcgtttcc gattgaatta ccatgatcac
     4801 cgaaaaatcg ttgtgatcga tggaaagatt agttataccg gtggtttcaa tattgccaat
     4861 caatatgtaa atacaacaaa aaaattcggc tattggcgcg atacgcatat acggattttc
     4921 ggtgccgctt cttctttgct ccagcttcgc ttcttaacag actggaacgt ctcggtacct
     4981 gaagaaaaaa aggtcggcta tcatttgaat tatttcttta aaaaagcaga tcgagatgaa
     5041 tctaagcttg ctgatacatc catccagctt gtttcaagcg gaccgaataa cgaaagggaa
     5101 caaatcaagc tttcatttat caaattgatt acttctgcta aaaaacgtgt ttggatacag
     5161 acaccttacc ttgttcctga tgaaagtgtc attgctgctt taaaaatcgc aactgcctct
     5221 ggtgtagatg tgaaaatcat gattcccaac aaaccggatc atccttttat ttatcgagca
     5281 acacaatatt atgctcggca gctgatcaag gaaaatgtac aaatccttgt ctatgagaac
     5341 ggcttcctcc atgcaaaaac attgataatg gatgatgaaa tctgcatggt aggttcagca
     5401 aatcaagata ttcgtagcta ccgattgaat tttgaaacaa gtgctgtcat ttacgatcct
     5461 gagtttttag aagaacttgc tactcagttc aaagaagatg agacacattg ttcatccatg
     5521 acaactgaaa cagtcaagga aatgtctaac tggctattat tcaagcaaca aatttctcga
     5581 ttattttctc caatcctata atttttttat tcaagtagag aaaaatatac taagattaaa
     5641 gggactgtga caaaaatctt gtcacagtcc cttcttccga a
//
LOCUS       DACECR010000133         5362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.134, whole genome shotgun sequence.
ACCESSION   DACECR010000133 DACECR010000000
VERSION     DACECR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.134"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..142)
                     /locus_tag="IWO17_002597"
     CDS             complement(<1..142)
                     /locus_tag="IWO17_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0291446.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="IWO17_002598"
     CDS             complement(183..437)
                     /locus_tag="IWO17_002598"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0291447.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="IWO17_002599"
     CDS             complement(548..802)
                     /locus_tag="IWO17_002599"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0291448.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="IWO17_002600"
     CDS             complement(995..1270)
                     /locus_tag="IWO17_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ0291449.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="IWO17_002601"
     CDS             complement(1242..2195)
                     /locus_tag="IWO17_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0291450.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="IWO17_002602"
     CDS             2807..4300
                     /locus_tag="IWO17_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ0291451.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="IWO17_002603"
     CDS             4435..4731
                     /locus_tag="IWO17_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0291452.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..>5362)
                     /locus_tag="IWO17_002604"
     CDS             complement(4755..>5362)
                     /locus_tag="IWO17_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0291453.1"
                     /translation="PSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEFGH
                     LEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFKSI
                     TFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVD
                     ESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1720 a          910 c          927 g         1805 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct
     4921 ctacgtaaca atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca
     4981 gcgaaataaa tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt
     5041 ccacaatcaa aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt
     5101 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
     5161 gataatcgct caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
     5221 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
     5281 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
     5341 ttcatcggta agtcagaagg at
//
LOCUS       DACECR010000134         5341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.135, whole genome shotgun sequence.
ACCESSION   DACECR010000134 DACECR010000000
VERSION     DACECR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.135"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(62..280)
                     /locus_tag="IWO17_002605"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IWO17_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IWO17_002606"
     CDS             complement(546..740)
                     /locus_tag="IWO17_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291454.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            2092..3132
                     /locus_tag="IWO17_002607"
     CDS             2092..3132
                     /locus_tag="IWO17_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0291455.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3956..4288
                     /locus_tag="IWO17_002608"
     CDS             3956..4288
                     /locus_tag="IWO17_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ0291456.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="IWO17_002609"
     CDS             4907..5248
                     /locus_tag="IWO17_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0291457.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1770 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttt tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattaa
     4861 tataaagcat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa ggggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgacgat taataaaaag gtagtattac cttaggcatt gagtaaggaa
     5341 t
//
LOCUS       DACECR010000135         5211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.136, whole genome shotgun sequence.
ACCESSION   DACECR010000135 DACECR010000000
VERSION     DACECR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.136"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            68..1186
                     /locus_tag="IWO17_002610"
     CDS             68..1186
                     /locus_tag="IWO17_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0291458.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1314..2690
                     /locus_tag="IWO17_002611"
     CDS             1314..2690
                     /locus_tag="IWO17_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0291459.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3091..4671
                     /locus_tag="IWO17_002612"
     CDS             3091..4671
                     /locus_tag="IWO17_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ0291460.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4869..5093
                     /locus_tag="IWO17_002613"
     CDS             4869..5093
                     /locus_tag="IWO17_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ0291461.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT         1769 a          840 c         1078 g         1524 t
ORIGIN      
        1 aaaaaaagaa taaaagaatt tttaacgaat ataatatata ctagtaagaa agaaaggagg
       61 aaatctattg tttgaaaaac ttagacagtc taaactaatg ttttggtcgc ttgaattatt
      121 gatcatagct gcattgatca tgatttcatc agaaataaac tttattttcg aaccgatcgg
      181 gacatttttt tccacgttat ttgcacctgt actgattgca ggctttcttt actatttatt
      241 gaatcctatt gtcaagctgt tgatgaaagc aaaaatcaaa agaatatggg ctgtcgcaat
      301 tgtgctgctc cttttagttg cagcaattat ttggatttta cttagtgtca tcccaagtct
      361 agtacagcaa atttcctcat tggcatctaa tatgcctgat ttcatcaaac aggtagaaag
      421 ctggttaaaa gaggtagccg aattgccttt gttcaaggaa gtagatatca ataaatattt
      481 tgagcaatta gacatttctt acgggacaat cattcagcaa ttccttagtg gagtatccaa
      541 tagtttaggt tcgatcgttg gaacgatcgc ttcggcaacc gtagtaatca ttacagcacc
      601 attcatcttg ttctatatgt tgaaagacgg agaaaaacta gtgccaaaca tccaacagtt
      661 tttcccagaa aaaagaagaa accaaattgt ggagttattg gggcaactga ataaaacatt
      721 ggctaattac attagcggac aagcaatcga atgtttattc gttggtacct tcacattttt
      781 aggatatatg gtaattggtg tggattatgc tttcttattt ggggtcattg cgggattgac
      841 aaacctaatt ccatatttag gaccttatct aggactagca cctgcatttc tagtaacagt
      901 cttcaatgat ccggtaaaag cattactttg ttgtgtggtt gtgctaatcg tccaacagtt
      961 ggatgggaat atcatttatc ctaatgtaat tgggaaatca ctgaaaatcc atccattaac
     1021 gatcattatc gttcttctag tagctggaaa tattgccgga ttgcttggta tcttcttagg
     1081 tgttcctttt tacgcaatct gcaagacgat catttcctat ataatcaaaa tcgtcaaaga
     1141 agacaagcaa aatgaaaaca agaagaaaat tgctacgacc tcgtaggtag ttttagtaca
     1201 cttgttttta ttgcaaagaa aattgctttg tgataccatt ttaacgtggc tatagtgcca
     1261 cgtttttttc tatcgtaaac taaaaattaa ttttataagg agagaatgcg attatgaatg
     1321 ctgaccctga gagtcagtcg ctgttagcgc agattttatt attgatcgtt ttgacattga
     1381 tcaatgcttt tttagcagct tccgaaattg ctgttgtttc gatcaataag aatcgaatcg
     1441 agcaaaaagc tgatgaagga gatgtgaaat ctcaaaagct tttaaaaata ttgcagaatc
     1501 caaataattt cctatcgaca atccaagtag gaatcacttt ggtcaacatt ttgtctggtg
     1561 cttcactagc taatacttta tcggaacggt tagctccggt attaggtggc ggcacagcgg
     1621 caaaaaatat tgcaaatatc attgtgctgg ctattttgac ctatgtatcg attgtttttg
     1681 gagaattgta ccctaaacgt attgctttaa ataaatcaga agaagtcgca aagttcactt
     1741 cagggctgat acgcttgatt ggtgtagtag ctaagccctt cgtctggctg ctttctgctt
     1801 ccacaagttt attggcgcgt atcacgccga tgacttttga tgatgaagat tcaaaaatga
     1861 ccagagatga aatgcgttat atgcttgaaa acgaaggcgt attgaataat gaagaattag
     1921 aaatgctcca aggggttttt tctcttgata cgaaagttgc ccgagaagtc atggtcccac
     1981 gaacggatgc atttatgatt gatatcaacg acactgtgga agaaaatgtg aatgaagttt
     2041 tatccgaaaa ctattcaaga atccctgttt ataacgaaga caaagataaa gtagtcggaa
     2101 tccttcatac aaagaattta ctaaaagctg cccataaatt cggctttgat aatttagata
     2161 tcaagaaaat catgcaggag cctttattcg ttcctgaaac ggtctttatt gatgatctgt
     2221 tatatgaaat gaaaaagacg cagaatcaga tggctatttt attagatgaa tatggcggcg
     2281 tcgttgggtt agcaacacta gaagaccttt tagaagaaat cgtcggagaa atcgatgatg
     2341 aatcagatga agttgagaat ctttatgaaa aaatcgatga acacgaatat atcatccaag
     2401 gacgtatgct gattgatgag ttcaatgaag cttttgacag tgatctgcat atgagtgatg
     2461 tagatacgat ggctggttat ttgattacag cattaggcat gattccggat gaaggcgaaa
     2521 agttatcgtt tgatgtcgat aatattacgc tggtttctga agaaatggaa ggttctcgaa
     2581 tccttaaaat ccgtgtcatt tttcatgatc cggaagaaac agaagcggaa cctgaagaag
     2641 aacgaagata cttcagaaaa gaattcgaag atgacgagcc aagaagataa ttggaaactt
     2701 ggttatgaaa gacgtgttgc ttgaataaac aagcaacacg tcttttcttt tagtcagctg
     2761 acaaaaaaat atttgactct tatggtttgg tggctttttc attcgctgaa tccatgctat
     2821 actgattcag ttgtagtagt tgacaaaggt tgtgaaaaag ctatcctgca ttaagtaata
     2881 agagcagcca agcaaaaaca gcttttcgta tttttggctt gggtgggctt attgctgata
     2941 ggctgtctct agaacgccgt ttactcggaa aaagagactg ctacacggct tttgtcacag
     3001 ccctatatga aattgtcttt ctttaagaga tcggacaatc tgtgcctaga gaaaatataa
     3061 ataattgaat ggaagaaatg gagttaggtt atgaataatc caaaattaaa gcaacaagtg
     3121 gacaatcgca gaacttttgc gatcatttcc catccggatg ctgggaaaac aacgattaca
     3181 gaacaattac tattgtttgg tggggctatc cgtcaagccg ggaccgtaaa aggcaaaaaa
     3241 acaggtaatt ttgccaaatc agactggatg gaaatcgaga aacaacgtgg gatttctgtt
     3301 actagttctg ttatgcagtt tgattatcaa gggaaacgag taaatatctt ggatacacca
     3361 ggacatgaag atttttctga agatacttat cgtactttga tggccgtgga tagtgcggtc
     3421 atggtaatcg atagtgcaaa agggatcgaa gcacagacaa agaaattatt ccaagttgta
     3481 aaaaaacgtg gcatcccgat atttactttt atcaacaaac tagatcgtga tggtcgtgaa
     3541 ccattggaat tgttagaaga attagaagag ctattagata tcgaatctta tccgatgaat
     3601 tggccaatcg gtatgggtaa agggctagaa ggtctttatg atatctacca taatcgtgta
     3661 gagttttatc gtccggaaaa ttatcaggat gaacgattag tagaattgga tgaagatgga
     3721 ctgcttcccg aaaatcatcc tctaactgaa aattccttat atgaacaagt cttagaagaa
     3781 gtagaactaa tcaaagaggc tggagatgca tttaatcaag aaaaaattgc tcgtggcgat
     3841 cagacaccag tattttttgg ttctgcattg actaacttcg gtgtacagac ttttttagag
     3901 actttcgttg aattggcacc agctccttat ggacacaaaa cacaagatga tcagatggta
     3961 agtccttatg aggaagaatt ttcaggtttc gtattcaaga tccaagcaaa tatgaatcca
     4021 gcccatcgtg accgaatcgc ttttgtccga atctgttctg gaacattcga acgaggaatg
     4081 gatgtctggt tggaacgtac aaataaaaaa ctgaaattaa gcaatgtcac acaatttatg
     4141 gcagattcga gagaaaacgt cgaaaaagca gtagcaggcg atatcatcgg tgtatatgat
     4201 acaggcaatt atcagattgg ggatacattg ttcgaaggaa aactaaaagt tgcttatgaa
     4261 gaactccctt catttactcc agagctgttt atgaaagtga ctgcaaaaaa tgtcatgaaa
     4321 caaaaatcct tccacaaagg aatctatcaa ttagttcaag aaggagcgat tcagctttat
     4381 aaaacatatc taacggaaga atatatcatt ggagctgttg gtcagctaca attcgaagta
     4441 ttccaatatc gtatgttaaa tgagtataac gccgaagtaa tcatgtctcc aatgggacac
     4501 aaaatcgctc gctggatcga tccagaagat ttggatgaga agatgagttc aagtcgcaat
     4561 atcttggctc gtgaccgttt cgaccaaccg ttgttcttat tcgagaacca atttgcagaa
     4621 cgctggtttg cggataaata tcccaatgta aaattaaaaa gtttgatgta aagaagtctt
     4681 gacacaagct gccctgcatc aagtaataag aatagccaaa caaaaacagc ctctcatatt
     4741 ttgtttagag ccgtgacgga ttttccgtga cggctctttt tactatatta aaatcataga
     4801 aatgtctaat caaaaaaata taaaaattat tttgcgtaca tatgattaaa gaaaggagag
     4861 tgtgacacat ggaaacagaa tggcaaagac gtgaaagaaa aaaacagaaa aaagaagaga
     4921 tgttacgatt atgtcgatta ttagatgtaa aagactggga caaaaacgag caaattgcac
     4981 aaaaagtacg atcaatcatc cataaagagg aaaaagttaa aaataaagac acgcgtagaa
     5041 ctgaaaatga aaagagaaaa gtacaaaaga aaagagaaaa atcaaaaagc tagaaagaaa
     5101 aagaggctga gacagaagcg tttaactcca agaaataagc tggaattcac aaaaatttga
     5161 aaagcaattt tcgtgaattt cagcttattt ccgaaggagt tgcttctgct t
//
LOCUS       DACECR010000136         5189 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.137, whole genome shotgun sequence.
ACCESSION   DACECR010000136 DACECR010000000
VERSION     DACECR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.137"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            137..748
                     /locus_tag="IWO17_002614"
     CDS             137..748
                     /locus_tag="IWO17_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0291462.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="IWO17_002615"
     CDS             821..1750
                     /locus_tag="IWO17_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0291463.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1908..2645
                     /locus_tag="IWO17_002616"
     CDS             1908..2645
                     /locus_tag="IWO17_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAQ0291464.1"
                     /translation="MLISCGARLAPFDIRELRELMSEDELELDKIGDRKTALFVIISD
                     TDDTFNFVVSIMYSQLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIAT
                     IRSREISASIILQAQSQLKAIYKDHADTIVGNCDSTLFLGGKEKTTVKELSETLGKET
                     IDLYNTSETRSNQKSFGLNYQKTGKELMSQDEITVMDGGKCIYQLRGARPFLSDKFDI
                     TKHKNYKLLEDYDKKNLFDVEEYLKQR"
     gene            complement(2693..3871)
                     /locus_tag="IWO17_002617"
     CDS             complement(2693..3871)
                     /locus_tag="IWO17_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0291465.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3894..3977
                     /locus_tag="IWO17_002618"
                     /pseudo
     CDS             3894..3977
                     /locus_tag="IWO17_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            4098..4499
                     /locus_tag="IWO17_002619"
     CDS             4098..4499
                     /locus_tag="IWO17_002619"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291466.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4706..>5189)
                     /locus_tag="IWO17_002620"
     CDS             complement(4706..>5189)
                     /locus_tag="IWO17_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0291467.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1639 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttt
//
LOCUS       DACECR010000137         5157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.138, whole genome shotgun sequence.
ACCESSION   DACECR010000137 DACECR010000000
VERSION     DACECR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.138"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(117..1079)
                     /locus_tag="IWO17_002621"
     CDS             complement(117..1079)
                     /locus_tag="IWO17_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0291468.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1081..2520)
                     /locus_tag="IWO17_002622"
     CDS             complement(1081..2520)
                     /locus_tag="IWO17_002622"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ0291469.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2705..4651
                     /locus_tag="IWO17_002623"
     CDS             2705..4651
                     /locus_tag="IWO17_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ0291470.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4915..>5157
                     /locus_tag="IWO17_002624"
     CDS             4915..>5157
                     /locus_tag="IWO17_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0291471.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1660 a          872 c          853 g         1772 t
ORIGIN      
        1 tctaactttt gggggtcact acaagttgac aactcctttt tgtttacaaa tcaattggat
       61 atctacaaat tgtattttct aacgaatttt atagacatta aaaaattaat ttaacgttaa
      121 atacttcctc cagaaagtat tgagattggt aaaatataat ccttatttgt cttttcgcct
      181 ttaatttttt tcagaagaat atcaacaata agacttgcaa tttcatcaat tggttgtctt
      241 attgttgtca attgtgggaa aaatttctca ataaaagagg taccatcgta tccaataatt
      301 tttaaatctt ctgggatatt aagtttaagt tgatgagcaa ttttcatagt gagaatggct
      361 gttaaatcat cagaaacaaa aataccatct ggtttgtgat ttataatagt tgactttatt
      421 tccatttcac gacggatagt agacagatta ttaggaattt tgaaaatttt tccatttgga
      481 ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag aatccgtgtt atcatttcct
      541 gtaatcataa tggtattttg acaaccattt ttttgtaaag tcttagctgc aagttttccc
      601 ccttcaaagt tatctgaaga aacaatggaa acattaggag ctaaatttct atcaaaagca
      661 acaataggtg ctctcacacg ctcataatca gcaattccaa gattatggct tgatgagatt
      721 atgccatcaa cttggttggc ttcaagcatt tcaatgtatt ctctctcttt agcaggatca
      781 ttttgactat tgcaaataat cgttttatat ccatgtttaa ataactctat ttcgagatac
      841 tcaatgagtt cagagtaaaa gatattacta atattaggaa aaataagacc aataagctga
      901 ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt tataaccaag agttttcata
      961 gcttcatgaa ctttttgttt tgttttttct gataaatatc ccttattatt aatgacgcgt
     1021 gaaacggttg ttgggctaac tccagctaat tcagcgacat cagttaattt tgctaccatt
     1081 ttaatatttt aattcaaagt attttccagt aggttcgcct gatttgattt gaataccatt
     1141 ttggtgattt tcagggaata cacgactagt aaacactttt tctcctttat taataaaaat
     1201 ctcaatgatt gagttatcta caaaaatgtt agcagttgtt tctttagtat caattgagca
     1261 ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct gctttgctgc gatctaaaat
     1321 aatttttcct tctttggtat ctacaatcaa gctaagtcca tttcctttac tatcagagaa
     1381 aagaagtaac tccgatttct gatttggagg aaaagttagt tcaagctcat aagtattatt
     1441 tgtttctgct tttgctgtaa attgttcaga actagcacgt aatgatgtaa tagcttctac
     1501 tggatattgg taaagtttac catcttttat tgaaagttct tttacaagac tcattgctcc
     1561 ctgataatca tacttatcag ttggataatc gatatcaggt agtccaatcc aactgacaat
     1621 cagagcacga ccatcaggag tattgaatcc ttgagtcgca tacgcttcaa atccataatc
     1681 aagattttga atttctgaag cgcccacaag ttttactttt tctatgtcaa gcgattgaca
     1741 gatcttaaaa gtattcggat agatattccc ataatttagc tcagatttat ccaacccttg
     1801 aggacaataa atgagaacag gttttttgtc tacaaatact agatttggac actcaatcat
     1861 gtattctgaa ccagtgccgc caaaatcaag gttgccaact tcctcccatg tttccacgtt
     1921 attgtcatcc gctttgtaaa gtttgataaa gcctgatttt tctaaatttt gtgctccaac
     1981 aatcgcatac aattgtcctt cataattaaa catttgtgga tcacgaaaat gatcagttac
     2041 atcagcaggt ttatgaataa gaacattctc acctttcgta atgttgtagt ctttatccat
     2101 ccatgctccg atttgaagtg tatcacgttc ccaattttca tttcgaacgt ttcctgtata
     2161 gagtaagaat agattcccct ctatttcata tgcagaaccg gaataagcgc cgtggctatc
     2221 tcgttttgtg tctggaagaa gttctgctcc tgttacatga aaatgaacta aatcatctga
     2281 ttcattgtgt acccattctt tcaacccatg agcagctcca aatggccaat tttgataaaa
     2341 aagcgtatat tttccgttaa aaaaagaaaa accattagga tcgtttaaca atcctgtttt
     2401 aggttcgata tgatagctag cacgccaagg tgatcgtttt acattatcct taattttatc
     2461 catctcatct ttagtccaat cagaataagc acgatatcgt atttctcgag gtaagttcat
     2521 attttactcc ttcaatttat ctttttacaa ctagtatggt aaacgttttc cgttgaaaaa
     2581 tcaatcattt catttaaaaa aataaactat atgacaaacg tttgacatat ttttaagata
     2641 tgctaaaata aatttgcatt tcagtgaacc gttttcacgt aaaaataaaa aaggagtgat
     2701 tcgaatgaat tacagtaaag tagcttccga cgtaatacaa gcggtcggaa aagataacct
     2761 tattgcagct gcacactgcg ctacacgtct tcgtctcgta ctaaaagatg acacgaaagt
     2821 gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc acatttaaaa ttgatggaca
     2881 atatcaagtg attatcggtg caggtgatgt taatttcgtt tatgacgaac ttatcaaaaa
     2941 aactggactt tcggaaattt caacagatga tttaaaacaa attgctagta aagataaaaa
     3001 attcaacccc attatggcat taattaaatt attatcagat atttttgttc caattatacc
     3061 tgcccttgtt gcaggtggtt tattaatggc attacaaaac tttttaacag ctgctggact
     3121 ttttggacca aaatcaattg aagaaatgta tcctgcgatt gaaggtattt cctcaatgat
     3181 ccaactgatg tccgctgcac cattcatgtt cttaccaatt ttagtaggta tttcagctgc
     3241 taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt ggtatgatca tgacaacacc
     3301 agatcttggt ggctcaactg aatattggaa tatttttggt tatcatgttt cacaaacaaa
     3361 ctatgcttat caagttattc cagttttagc agctgtttgg atattatcta tattagaaaa
     3421 attctttcat aaaaaattgc catcatctat tgactttaca ttcacgccat tattatcagt
     3481 aatgattact ggatttttga ctttcacagt tattggccct gtaatgttat tgttgtctaa
     3541 tggaattaca gacattattg tttggctata taacactact ggatttattg gaatgggtat
     3601 ttttggagga acatattcac ttatagtcat gactggttta catcaatcat ttccagctat
     3661 tgaaactcaa ttactttcag cgtggacaaa tggtatcgga catggagact ttatctttgt
     3721 agttgcatct atggcaaacg ttgctcaagg tgctgcaaca tttgccattt ggttcttaac
     3781 taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt gtctcagcac ttcttggaat
     3841 tacagagcct gccttgttcg gagttaactt aaaataccgc tttccattct tttgtgcttt
     3901 aattggttca ggaattgcta cagcagtagc aggattactt aaagtagttg ctgtttcact
     3961 tggttcagca ggtttcttgg gatttctttc aattaatgca acatctattc cattttacgt
     4021 attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt acttacttct acggtaaaac
     4081 aaaagctgct ggtatcttta aagcagaaga aattgctgaa caaacaccag ttaattcttc
     4141 aaaagtagca acaacagatc caatatctac tgatgttgaa tcaacacaaa caaaagtttc
     4201 atcagaaaca attgttagtc ccatttctgg taaagtgatt agtctttctt cagtaaatga
     4261 tccagtcttt tcatcagaag ctatgggaaa agggattgcc attaaaccaa atggaaacac
     4321 ggtccattct ccagtagatg gtactattca actcacgttt gaaactggac atgcttatgg
     4381 tattaaatca aataatggtg ctgaagttct aattcatatt ggtattgata ctgtatcaat
     4441 gggaggaaaa ggatttacac aaaaagttac tactaatcaa aaagttagaa aaggcgatat
     4501 tcttggagca tttgaccgtt caatgattga gaaagaaggt cttgatgata caacaatgat
     4561 tattgtgacc aatacttcgg attattcaga agtaaatata gtagctgaaa atacagtcac
     4621 tgagggtact gatttacttg aaatcaaata aatatatttc cctgagtaaa gatggaattt
     4681 tctttccaaa atcaaataat atagattaat caaatatttg agaataacat tttatttaat
     4741 cttttcatat atcaattgta tgaaaaatta ttagaatttt aaaaaatgat aaaaattcaa
     4801 aaagaaaaaa tagtagttat ttacttgtca tgttactaaa taaaaaaata atttaaatat
     4861 gatatatagt catttattta gtgatttcat tctaaaataa agggagaaat gtctatgcta
     4921 tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg ccattggcga tgaagaaatg
     4981 acgatcaagg aaagagtgag ctttccgaca acgacacctg aggaaacaat gcctttagtc
     5041 attgacttct tcaaacaata ccaagcagat ttagcaggga ttgggattgg ctcatttggg
     5101 cctatcgata tccatcgcga ttccgccact tatgggtaca ttacctcgac cccgaaa
//
LOCUS       DACECR010000138         5137 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.139, whole genome shotgun sequence.
ACCESSION   DACECR010000138 DACECR010000000
VERSION     DACECR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.139"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..447)
                     /locus_tag="IWO17_002625"
     CDS             complement(<1..447)
                     /locus_tag="IWO17_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0291472.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
     gene            complement(754..1851)
                     /locus_tag="IWO17_002626"
     CDS             complement(754..1851)
                     /locus_tag="IWO17_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0291473.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1959..3986)
                     /locus_tag="IWO17_002627"
     CDS             complement(1959..3986)
                     /locus_tag="IWO17_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ0291474.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4131..4862)
                     /locus_tag="IWO17_002628"
     CDS             complement(4131..4862)
                     /locus_tag="IWO17_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0291475.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1456 a         1040 c          813 g         1828 t
ORIGIN      
        1 caactttctc ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa
       61 tactttataa gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagat
      121 atatttttcc tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg
      181 agataacaga gctttgctag aacattttcc attaatttcc agacatatat ccaattcact
      241 gacggttact cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc
      301 gatcgttttt ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga
      361 ctctgataaa tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc
      421 tttgcttaaa aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc
      481 catgcattca ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc
      541 agcgattctt gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg
      601 ttttatttgg cctttctcgc gatcaaacct gttattagaa ttatatcatt tactagtatg
      661 catttttatt aataaaatgg tatattttat attgttataa aaagagccca accatcctac
      721 aataataaaa atttactatt tgttatctta accttacttt tctttctctt ctttttttct
      781 caaaacaaac aaaagtatgc ataataaaat aaacaataag aaaagtgaag ctactaagat
      841 ataaatccaa ttattgtctt ttttaatcgt aacatccgtc gtgttcaatt ccttggctac
      901 ttttccgtct attgtaaaat ctttttcaaa tacccattga ttggtatagt tgatcgtagt
      961 atttcctttt tttctcgaaa attttccatt ttcattttca tttcctaaaa cagtcattgt
     1021 aaggtgatat tcacctgggg ttagcggttg accatttaaa gcagttggaa aagaaaaatt
     1081 tgtattcggc gcaatctgca atccttcttt ttcctcttga tataaaactt cagaatgtcc
     1141 tttttttgta atttttgttt caataactac ttgattgata taggtttttt gatcattttg
     1201 aacgtttgct aagatgacat ttcttgcatt gatttgatct ggtttaactt catgaagagt
     1261 aaggttcgga gcaacttcgt tcagtgtctg ttgcatcaac aaagcaataa tataggtgta
     1321 ttcattctca atcgataagc tttgatcctt agtttctgtt tggtcctttt ccgtctggat
     1381 ttcttggaat gtgatcccac cagccaatac accatcaaat cgctcattcg gcatcctgac
     1441 atgaaaagca acggtttgga cacttttagg ttttagcgtc actatctgtg gatagctcac
     1501 ataatctacc agatcaaaga tcaggctcat gtctttttca atctcgtttt ttccgtattc
     1561 aaccatcaca tttgaattgg tggttgcgct attcacagaa atatcgattt tcacttcttt
     1621 ttctgtatca tttctcaatt ccgcttttac ttctacttcc tgatccggat caagttgaag
     1681 atcgaagtaa gtttttctct tgtcgatttg attctctgaa gttatcggcg tcactgaaaa
     1741 attaaatgca ctagctgaca ctgatatatt gttaaaaagt gcccctaaaa taactaggag
     1801 tgttataaat aattgttgtt ttggtctcaa gaaaatcgct cctttaatca tatgtaagag
     1861 aagactataa caaaatcttg tctctgtggg acacaagagc ctatggtacc tgtgcccttc
     1921 ttgaaactca ataattttgt tatattcttc tcttcctgtt aatttcctgg gacatctgca
     1981 agtatccaag atagtttccc ctcaaaggtt ccgattcttt ggttttctac aggaatattt
     2041 attctgattc cacgatgagc tgtagaatct aaaagagagg acaaatcgct ttcacccttt
     2101 tgatcacttt cgaaaatcgt aacagcggat gtccctataa ccgttgtatt ttcagaggta
     2161 acaaaggaaa gagcttctgg aagctcaaaa tctccatttg taagaacttt ctcttgtttc
     2221 aattgtaatt tccaacttcc aaaagcccgt tcatctataa ttttcaaccc acctgaaaac
     2281 tgtccgaatt cacttaatgg tctactggat attttatgtt ttccaaaatt gacatattga
     2341 ggagcagttg agagataaag tttgcctttt ctgtaataaa aatagatatc ctctgtattt
     2401 tcggagaaga tgccttctgt attcgctgaa tccgagtcaa aaacataccc ttcaatatca
     2461 ataggagttt ctttatactt ctctcctatt ttcccagtga ttgttttcat ttcagcaata
     2521 tcaatctgac tgtctttatc tttataataa acatggatat ctatctcctt cagctttgcg
     2581 atctttaggt ttatttcatt cgattgctca atcaaattcc cgttttcaag gtattcaaaa
     2641 tgcatagtga gtactttttc aggaattact tcctgcaaag ttgtctccaa ttcgattatt
     2701 aacgtatctc cagcaataaa ataatctgtc agatttagcg tgactcgatt tccggttatc
     2761 aagatatctt tggcatcaat ttgatttcca ttgatcaaga tatgctcggg aatagttaat
     2821 tcttgtggga ggtcgatact agtctgcatt ttcttatatt caagaaaatc cccctcgacc
     2881 ttcaatgtat ttctgatagt tatcttatcg tttcctccag cttcagcgat ggccccataa
     2941 tcactatcgt tgacttttac atcagctgtt gatgtgacat tcgatggaag tcgcgtgaca
     3001 gcgacagcat tttcttgaaa aaaagtacct gttgaagaag taaaccccca atttacttct
     3061 gtagaaccaa actgcctaat gagatcaggt ataatccgag aagagcgacc gacaccttcc
     3121 aaatcataag taagacttct ttgctccgcg tcccatttaa ttattaacaa gcgccatgtg
     3181 ccattagaaa gaaatgtagg agctaccgtc acgccagtat gctctccaga ataattatta
     3241 ttatttggtt taggcgtgac aaaagcgaca tgtccatgcc ctctattatc agcagttatc
     3301 tctcgatcca ttcgatcaga agaaccacta tttttatacg tatcaaactc aatcgagagc
     3361 gcattcgcaa tgtaaggttg gccagaacga gtagaatatg agccaattcc catacctggc
     3421 gatccaataa cttgactggg attactagcc attctaggat cgccagtcaa tgtaaaggtc
     3481 atgccatctc ccgctctatc tcgttgattt cctaagtata aataagattt aaatgtaaaa
     3541 tccgagagta aactaatctt cttatttgac caaatagctc ctctttgaga cctttctgct
     3601 gggttcaact gcagtaactt cccattttcc aatatggacg tgctagtacc gataggtgtt
     3661 tgaaaaattt tatcgataga aataatattg ggatcgtcag gcagctcaac cgcagaaatt
     3721 ggtgcatctt ctagtccttg tttgtccgaa ccctcagctt ctacgttgtc agtggtctct
     3781 ccactactcg aaatacttaa atcctcactg ctttcttgtg tctggttgga ttctattaaa
     3841 ctgtcgaatg tcgaagtgag tttgctttct tcgtctcttc tatttccatg ctccaactga
     3901 ttcaattcca aagagtaccc tatgatcgga aagatattag tcactaagag actgcttaaa
     3961 atgagcacga tcctcctctt tttcaatata tcttttcctc ctgatcgtta aattcctaga
     4021 tgaactattc gtatccaaac aaatttagcc tagcctttga caatttttgc tcgattacct
     4081 cgcaagacaa tatgactacg tatttatttt taacagttct tttcagtaaa ctagttgtta
     4141 ggtgtatctt ctaatgtcca aactaattta gtggaatatt gttgcgctaa cttgacagat
     4201 ttaccgggta ccgaaagctg taccgcatct tgattttcat tttcatttgc accaaatcga
     4261 tagacccaag tccccacacc tgttcctgat ttagcattaa ttgctaacgt attgtttcct
     4321 cccggtgtca acgttaattc agaattcacc gtttgcggcg tatatgttgg atcaaccaaa
     4381 gaaacagcct gccctttcgt aaatttcagc tgtgctccaa ctagttcatc cccactggca
     4441 gttttaaact gttctggttg agagacactt aatttccacc cagataaggt acttctccta
     4501 tctgtcactt ggacatatgt cggttttcta gacccatcgt tcatcacatc agcagctgca
     4561 taatacgttt tatccgcagt agaaattttt tgagtcccaa atttgaattt tgatccataa
     4621 tcaatcgata atgcccctcc tgttccaggg cttggaggat ctgatggatc gacaggttta
     4681 ttgggatcag gattctctgg atcaacagga ggagttacac cctcatctcc tgcttcaaat
     4741 gtaatcgttc cttctgtgtt atattccttt gggtatactt cagttgcaga aacaaaagtc
     4801 gtacttgcta ccatgctgaa taacatcatt gctgctgtta atacgatttc ttttttcatc
     4861 attgacattc ctctttcttt atttactaaa catatcagtc gattggatta atccatagca
     4921 aaattctgtt ttacgatact tatgaaataa aagaacatag acctatattt ggtcctatgt
     4981 atcgattagt tataaatgat tatcagcccg atttgatttc tagtttttat tgcaacttga
     5041 tgcaaacaca atctagaaat acagctgtca tagcctttaa aataattcgt aagtccccct
     5101 cctatctatc aaaatgtgac ttgacgagag tgtaaaa
//
LOCUS       DACECR010000139         5052 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.140, whole genome shotgun sequence.
ACCESSION   DACECR010000139 DACECR010000000
VERSION     DACECR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.140"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..424
                     /locus_tag="IWO17_002629"
     CDS             <1..424
                     /locus_tag="IWO17_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0291476.1"
                     /translation="VHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGV
                     KARNVKTGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFA
                     IGDVREKDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            638..2365
                     /locus_tag="IWO17_002630"
     CDS             638..2365
                     /locus_tag="IWO17_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0291477.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            2438..2731
                     /locus_tag="IWO17_002631"
     CDS             2438..2731
                     /locus_tag="IWO17_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0291478.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(2826..3686)
                     /locus_tag="IWO17_002632"
     CDS             complement(2826..3686)
                     /locus_tag="IWO17_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ0291479.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            3778..4206
                     /locus_tag="IWO17_002633"
     CDS             3778..4206
                     /locus_tag="IWO17_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0291480.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            4368..4544
                     /locus_tag="IWO17_002634"
     CDS             4368..4544
                     /locus_tag="IWO17_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ0291481.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            4571..5017
                     /locus_tag="IWO17_002635"
     CDS             4571..5017
                     /locus_tag="IWO17_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ0291482.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         1709 a          903 c         1074 g         1366 t
ORIGIN      
        1 cgtccaccgt agagatgaat tacgtgcaca aaagatcatc caagaccgtg catttgcgaa
       61 tgaaaaaatt tcctttatgt gggatacagt cgttgacgaa atcgttggga atgaaatggt
      121 agtgacaggt gtgaaagcac gtaatgtcaa aaccggcaat gagtttgatt tggcagcaga
      181 cggagtattc atctatgttg gactggatcc attaactgca ccatttagag aagcagggtt
      241 gacgaacgaa gctggctgga ttccaacaga caacgaaatg aagacgaata taccaggcgt
      301 ttttgccatc ggagatgttc gtgaaaaaga tttgcgacaa atcactactg ctgttggtga
      361 tggtgggatc gctggacaac aagtgtataa atacttggaa tctttagaag aaacaactgc
      421 ataagtttaa aaagagtgga agaacggttt caattgttct tttactcttt tttttcgcgt
      481 gaaatctact gaatccgatt tatgcgaata cttgcaaccg tgcttgataa aagaatcctt
      541 tcttgattta agcgtttttt ggtcctcata cttttatatc taataagtga atgctatact
      601 tattaagaac catatttcag aaagaggtgt tattttcatg tcttgggaac aagtgtatga
      661 acaatgggta catgaagaaa atttagaaga aaacttaaaa aaacaattaa atgatctggg
      721 tgaagatcct gaaaaacttg aagatgcttt ttatgcaccg ttggaattcg gtactgcagg
      781 catgcgggga atcttagggc ctgggatcaa tcgaatgaac atctatacgg taagacaagc
      841 aacagaagga cttgcacgtt tcatggatac acaaggcgaa gaaacaaaac gccgaggtgt
      901 cgctatcgct tatgactcac gccatcaatc accagaattt gccatggaag cagccaaaac
      961 attagcacat cacaatatcc catcttatgt attcgaaagt ctacgtccaa caccagagct
     1021 ttcttttgca gtccgtcatt tgaagacgtt tgcgggaatc atggtaactg catcccacaa
     1081 tccagcagct tacaacggat ataaagtcta cggcgaagat gggggacaaa tgcctcctgc
     1141 agacgcagat gcattgacga aatatgtccg tgaagtcagc aatccgttaa aagttgaagt
     1201 attatctgac gaagaagctg aacatagcgg attgatcacg atcatcggag aagaagtgga
     1261 tgccgcttat ctagaagaaa tcaaagcagt aacgattgat catgaattgg tggaaacaat
     1321 ggggaaagat ttgaaacttg tgtacacacc acttcacgga acaggaaaaa tgttgggcga
     1381 acgtgcgttg aaacaagctg gattcgaaca attcgtacta gttccagaac aagctgtcgc
     1441 agacccagat ttcactacag tcaaatcacc aaatccagaa gaacactctg cttttgagta
     1501 tgcgatacga cttggcgaga aagaaggcgc tgatttgctg attgcaacag atcctgatgc
     1561 agaccgttta ggtgcagccg ttcgtttgcc aaatggctcc tatgaagtat tgaccggaaa
     1621 ccaaatcgga gcactgtttg ctcaatatat tttggaagca caccaatcgc gaggcacttt
     1681 acctgaaaac gcagcggtat tgaaatcgat cgtttctagc gaattaccta cagcaattgc
     1741 ggaacactat ggggctacca tgttcaatgt attgacagga ttcaaattca ttgctgaaaa
     1801 aattcaacaa tttgaagaag atcattccca tacttttatg ttcggattcg aagaaagtta
     1861 cggttattta gttaaacctt tcgttcgcga caaagacgcg atccaagcat tgctcttatt
     1921 agcagaagta gcagcttact ataaaaaaca aggcaaaacg ttgtatgacg gcttgcaaac
     1981 gatttttgaa acctatggct actacgcaga aaaaacgatt tctgtgacaa tgagcggaca
     2041 agaaggttct gcgaaaatct ctgctttgat ggaaaaattc cgtgaagaag caccaacaga
     2101 atttgccgga gcaaaagtca tccaaacaga agacttcaaa gagttgacga gaacagaccg
     2161 tgaaggaaac gtagaaaaac taacgacacc gccatctgat gtattgaagt atacgctgga
     2221 agaaaatggc tggatcgcta tccgtccttc aggaactgag ccgaaaatca aattttacat
     2281 cggtgtaaaa ggaaactctt cagaagacgc agctgaaaaa gtagccgctt atgaagcgat
     2341 tatccaaaag atgacagaag aataaaaaaa cttgagactg atggaaaaag tggtaaactt
     2401 tagaagtcag tcttttttca agttggaggt gaatggattg ccagacacgc aaaaaacaaa
     2461 agtagagaaa gtcagccaac tattcaaaat gttgagtgac cccactcgtc tcaaaattct
     2521 gctgtatctg aaagacgggg aacaaaatgt gacagcaatc acccaagcag tggaaatgga
     2581 acaatctgct gtatcgcatc aactgcgttt attaagagaa aaccatgtcg tcaagtccca
     2641 tcgtgaggga aaagcgattt tgtatagtct tgatgattct cacgtactgg atattttgaa
     2701 ccagacactc aaacatgtgg aacatcaata acgataggtg caaatagaga caaactggac
     2761 tgtgtcaacg aatcttcttc gctgacacag tccagttttt tttaagaaaa agacaagaaa
     2821 aactgttagt ctgtttcttc ggtggtttca gttccataat aactatgatc ttctaggtct
     2881 aaagcattca aaataagcga agccgtttct tcaatagata aagatgccac attgatcact
     2941 acacatccta gtttttcata aagatcattc gcaaaatcga gttcagctct aattttttcg
     3001 atatcggaat aagcagtatc agggtttaat ccgtaagagc gcatccgttc tttacggata
     3061 ttatttaaaa tatctgggtt attcgttagt cccacgattt ttttaggatc gatttcccac
     3121 agctgtttgg gaatatgtgc ttggggaatc aaaggtaaat tagcaacttt taaattttta
     3181 tttgctaaaa acaagctcaa aggcgttttg gaagttcggg atacgcctag caaaacgaca
     3241 tctgcttcta aaaaaccacg aggatctttc ccatcatcgt attttacagc aaattccatt
     3301 gctttgatcc gtttgaaata gttctcattc aataaatgga gggcaccagg ttgccgaatt
     3361 ggcttgatac cagttaaacg ttcaatttct gatatcggtg gcgtcatcac atcgaaacta
     3421 aataattgat gttcttcaca aaaagatcgt gttatctcga tcagttcttg attgattaat
     3481 gtatgcagga caagtgcttt ttcttctaat gcatcttgca atgcctgcct taatgttttt
     3541 tcatctgtca cgaacgttct gcggaatagg ttgaactcaa cggtaggata ctgagccata
     3601 gtagcttgcg ctagttttgt cgcagtttct ccagcagagt cggagatgac aaaaaaagta
     3661 acgatttctg tagaatcgga agacatggat aaaacctcac ttgcattttt tttatgttta
     3721 ttataccata ctctagaatg cgtacttcgt aatcattttg atttggagga ataagtcatg
     3781 aaaaaacaga cattgatcga acaatcatta gaaacattga aaaataacgg ttttaaatat
     3841 actaaaaaaa gagaagcatt gttaacttat ttagtgaagc gtaaccgcta cgtatctgct
     3901 aaagaagttt ttgactatat gaatcaggaa ttcaaagggg taagttatga tacgatttat
     3961 cggaatctcc atgattttgc agagatcgat ttactggaag aaaccgattt aaatggagaa
     4021 atgaagtttc gttttcattg ctgtcagggg gaaatcggcc accaccatca ttttatctgt
     4081 acagtgtgcg gaacaacgaa agaaatccat atgtgtccga tgaattattt cgaagaacag
     4141 ctaggtggat gcacgatcga agggcataga ttcgagattt tcggaagatg tgaaaattgt
     4201 caataaggat ttaaataaaa taacatttga taatttaaaa aaaaagcaag atttccttga
     4261 cggtcaaaac agcattagat ataatattta caggacagtc ttgttttttg agatatattt
     4321 caataacaat tacggttact ttttaagctc ggagggaggg aattaacatg tcaaaaacag
     4381 tcgttcgcaa aaacgaatca cttgacgatg ctcttcgtcg cttcaaacgt tccgtgtcaa
     4441 aagcgggtac tttacaagaa tctcgtaaac gtgaattcta cgaaaaacca agtgtaagac
     4501 gcaagaaaaa atctgaagca gctagaaaac gcaaaaaatt ctagttctgt gatgaattgg
     4561 agttggtagt gtgtcactac taagtagatt aaacgatgac atgaaagcag cgatgaaagc
     4621 gaaagataag gaatctttac aagtcatccg aatgatcaag tcatctatac aaaacgagca
     4681 gattaaagaa ggtcacgact tgacagaaga agaagaacta acggtgttgt ctcgcgaaat
     4741 gaaacagcga cgtgattctt tgcatgagtt tgaagaagca ggacgtgacg atttagctga
     4801 aaaagtaaaa agtgaaattg taatagtcga aaaatatatg ccggaacaac tttccgacga
     4861 agaaattcga caacttgttc aagaagcaat cactcaaact ggtgcttcat caatgaaaga
     4921 atttggcaaa gttatgggtg caattatgcc taaagtcaaa ggaaaagcag atggaaacca
     4981 agtgaatgcg attgtgaaag aactattaca atcttaattt tatcatttaa actgcggaca
     5041 gtgattgtcc gc
//
LOCUS       DACECR010000140         5004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.141, whole genome shotgun sequence.
ACCESSION   DACECR010000140 DACECR010000000
VERSION     DACECR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.141"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..104
                     /locus_tag="IWO17_002636"
     CDS             <1..104
                     /locus_tag="IWO17_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HAQ0291483.1"
                     /translation="LLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            792..1613
                     /locus_tag="IWO17_002637"
                     /pseudo
     CDS             792..1613
                     /locus_tag="IWO17_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1756..1980
                     /locus_tag="IWO17_002638"
     CDS             1756..1980
                     /locus_tag="IWO17_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0291484.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            1995..2864
                     /locus_tag="IWO17_002639"
     CDS             1995..2864
                     /locus_tag="IWO17_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0291485.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2845..3579
                     /locus_tag="IWO17_002640"
     CDS             2845..3579
                     /locus_tag="IWO17_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0291486.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            3612..4520
                     /locus_tag="IWO17_002641"
     CDS             3612..4520
                     /locus_tag="IWO17_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ0291487.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4517..4765
                     /locus_tag="IWO17_002642"
     CDS             4517..4765
                     /locus_tag="IWO17_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAQ0291488.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
     gene            complement(4768..>5004)
                     /locus_tag="IWO17_002643"
     CDS             complement(4768..>5004)
                     /locus_tag="IWO17_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0291489.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
BASE COUNT         1690 a          757 c         1084 g         1473 t
ORIGIN      
        1 ctttgctatt agttatggat ttgcaaagtc accttattga acaatacaac ttaaaagtta
       61 cacatgacat tttagaaaga ttgttgaatt acattagtga ataggagagt aacgttattc
      121 acagtattgg gtactgaaaa ttaacagcat acctcagttt ttcaaaaaac agcgaagcct
      181 atggatatgt tcccgtctac cgatagtgca aaaagtgtcg tggatatgtt tccgagaacg
      241 gacatacaca aatgacattc attctgtcct ctcaatccat gtggaagcag tatgtctgtt
      301 aacaagaaaa taattattgt tcgtcctgac gagttgttag actcgaaaag gataaacatc
      361 atttttagaa ccggtagccg atttttggct agcggttttt tgggctcttt ggcaaatagt
      421 ggtgattgaa tctcgcgcta tcctatggaa aaccgccttg tgtgaggggc tgcgagaccg
      481 cttcgatcct agaattgctg aagcacgaat ctagcgtcac tcacatttca tcttttgacg
      541 tagctgagac aaggtgtttt tcttcgatcc gctagtggtt aatctctttt tggtgcaaat
      601 atggtttaga atttctttgc atggcttaag ggagtggaga atcggatatt agtaggattt
      661 acatccacag ataaaaatag gcttgatttt aacagcgaaa ctaaaatacc ctacttaaat
      721 gaagtgatag attagatgtt gggttgttag gagatgctta aaaatcctat gcaatcaata
      781 gtttttagtg tttgttgact tgatccctaa atcgcctgag tactaaacgt tgagtgtgtc
      841 tgcttactgt cactagccag taagtgagac tgtagttaat ctctcatttt ccccagtatt
      901 atgttgaaaa tagccatgaa gcgattattt ctaaagagtt atttttgcag gctcaagaag
      961 aaatttatcg gagaagtaat atctacacag gagaaggcaa gaacaaaaga atttatagta
     1021 gcaagtacgc tttaagtgcc atcaccttct gtggatattg tggcgatatt tataggagaa
     1081 cctattggaa tattcatggc agaaaagaat ttgtctggcg atgcgtgact agaatcgagc
     1141 agggtcccga ggtatgtaag aaccgaaccg taaaagaaga tgaactctat ggtgcggtaa
     1201 tgaccgcaat taataagcta cttgctggtg gcaacaatat gataaagaca ctggaagaaa
     1261 atattcatgc tgtgattgga gaaaccacag aataccaaat ttcagagatt aataccctac
     1321 tagatgaaaa acaaaaagaa ctaatcaagc tggccaataa aggtcaagat tatgaatacc
     1381 tggtagatga gatcgatgag atgcgagata agcgtcagac tcttttagta gaagatgcct
     1441 ccctcagtgg cgagaacgag cgcatcaatg agttgattga atttatccgc aagaacaaat
     1501 tccgcacctt agaatatgat gataagcttg taaggaagat aatccagaac gttaaggtct
     1561 atgaagacca cttcgtcata gccttcaaac caggtatcga aatggaaata tgaaaaccag
     1621 aagtaaatag cccatgactc tgaagtagag ttgtgggttt tcttttaatt gctgattata
     1681 ataatatttt aatgactatt gcatttatca accaaatggt ttatgttatt cttagcataa
     1741 acttggaggt gctgtatgac tacggcagaa atgattaaag aactgtgtga gcaaatgaat
     1801 ataagtgttt ccgaacttgc tagacgtatt ggccagactc cacagaattt taataaaaaa
     1861 ttacaacgag aaacagtaac cttggatgag ttgaaggcca ttgctgatgt gctaggtgtc
     1921 aagtttgtgc aggcatttat tttaccagat ggagatgaaa taaaaatatc taacgaataa
     1981 aggaggatgt ttatatggta ggtaatgtta ttcaaatagt aacagagaaa ttatcatctt
     2041 tgccttttat agaaggcatt gtattagggg gctcacgtgc aagaagcacc catacagaaa
     2101 attctgatat agatatcgga atctattaca attcagactc atttgacctg acagcgatta
     2161 atcaaattgc tacagagttg gatgatgaga atagaaacaa ccttgttgta cctcccggag
     2221 cgtggggtga ttgggttaat ggcggtggat ggttagttat aaacgggtgt catgttgact
     2281 tgatattacg tgatataaaa cgggtggaac aaataatcaa agatacggag caaggaattg
     2341 ttactgccaa ttatcagacc gggcatcccc atggttatat aagtgctatg tatcgtggag
     2401 aattagcgat tagtaaaata ctatatgcta agaacgaaag cttatgcgaa ttaaaaaagc
     2461 aggcagaaat ttaccctact gctctaaaga aaagcttgat gaactttttt atatttgaag
     2521 ctgaattctc tttaatgttt gtaaaagcaa atgcgggggc agaggataaa tattatattg
     2581 caggccatgt ttttcgtatt atttcatgct taaatcaagt actatttgca tgtaataatg
     2641 cttattgcat taacgaaaag aaagctataa aactgcttga aacttttgaa tataaacctg
     2701 aaaaatatgc cgaaagagtt aatcatattt ttgaagtact cggtctttct ctttttgaat
     2761 gctacgacat gaccgagaaa ctttataaag aagtgaatga aattgtatcg gagataaata
     2821 actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa tatatttttt
     2881 caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg agaatgggag
     2941 actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt aggatgcggc
     3001 tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt aggtgttgat
     3061 atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca gattgaatat
     3121 gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt aatactaagt
     3181 tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat atataggatg
     3241 ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac tgctcatgga
     3301 acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt ggacaattat
     3361 tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa atatcataga
     3421 acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa tcagattgtg
     3481 gagccacagc cgccagagaa catgatggat attccgggga tggcggatga aatgcgacgc
     3541 ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa aaataaacga
     3601 ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct ttagcagaac
     3661 aggatgaacg tattcgaatt gtgacccttg aggggtcacg cgcaaatatt aatataccta
     3721 aagatgaatt tcaggattat gatattacat attttgtaag tgatatagaa ccgtttatat
     3781 ctaatgatga ctggcttaat caatttggga atataataat gatgcaaaag ccggaggata
     3841 tggaattatt cccacctgaa gaaaagggat tttcctatct tatgctattt gatgattaca
     3901 ataaaattga tcttacctta ttgcccttgg aagagttaga taattaccta aagggcgata
     3961 aattaataaa ggttctaatt gataaagatt gtagaattaa aagggacata gttccgactg
     4021 atatagatta tcatgtaaga aagccaagcg caagggagta tgatgattgc tgcaatgaat
     4081 tttggaatgt aacaccttat gttattaaag gattgtgccg taaggaaatt ttatttgcta
     4141 ttgatcattt taatcagatt gttcgccatg agctgctgag aatgatatca tggaaggtcg
     4201 gcatcgaaac aggctttaaa ttaagtgtag gcaagaacta taagtttatt gaaaggtata
     4261 tatccgagga tttgtgggag aaacttttgt ccacctaccg gatggattcc tatgaaaaca
     4321 tatgggaagc attatttcta tgccatcaat tgttcagggc ggtatccggt gaggtggcgg
     4381 aaaggcttca ttatgcctat ccggagtatg ataggaatat aacaaaatat accagggaca
     4441 tgtataaaaa atacactggt aaaaccggct gcctggatag cacatatgcc gctgatatag
     4501 aagagaggcg ggaacagtga ttacagaaat gaaagcaggg cacctgaaag atatcgataa
     4561 acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa acgagaattg
     4621 gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag acgaagagga
     4681 tgaagaggat gaggaggcag attgccttga atatattgac aatactgata agataatata
     4741 tcttttaacc cgtggtgcta gttaatttta atatcctaaa gaatatctca aagttcctcc
     4801 aaaacgtcgt tgtgctcgac ttaatagaac actgtaagcc agtaatatgc tttcaaatct
     4861 actctctagc cctttaacag aacgtgagtt gaaattttga caacctaatt tttctaagga
     4921 agaaaaaaca gtctcaactg tttttctttg ttttccaagt aagctgtggt ctagtttttc
     4981 tgatttatcc atgtttttac gtgg
//
LOCUS       DACECR010000141         4774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.144, whole genome shotgun sequence.
ACCESSION   DACECR010000141 DACECR010000000
VERSION     DACECR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.144"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            102..275
                     /locus_tag="IWO17_002644"
                     /pseudo
     CDS             102..275
                     /locus_tag="IWO17_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
     gene            272..1246
                     /gene="lacD"
                     /locus_tag="IWO17_002645"
     CDS             272..1246
                     /gene="lacD"
                     /locus_tag="IWO17_002645"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0291490.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDTTIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGILCGR
                     ATWAKGVAPFVTEGEKKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(1425..2375)
                     /gene="yidC"
                     /locus_tag="IWO17_002646"
     CDS             complement(1425..2375)
                     /gene="yidC"
                     /locus_tag="IWO17_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0291491.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(2971..3246)
                     /locus_tag="IWO17_002647"
     CDS             complement(2971..3246)
                     /locus_tag="IWO17_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ0291492.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            3353..4117
                     /locus_tag="IWO17_002648"
     CDS             3353..4117
                     /locus_tag="IWO17_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ0291493.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            4240..4743
                     /locus_tag="IWO17_002649"
     CDS             4240..4743
                     /locus_tag="IWO17_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0291494.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
BASE COUNT         1509 a          870 c          992 g         1403 t
ORIGIN      
        1 ttggtggtcc cttaaaaagc ccctcttaag aggataagca agtgatgagg ccttctaggg
       61 ctgccaaaaa ccacccgttt tcacgagtag atatttacta ttttattttt gcgttattta
      121 ttccagattc agaagcagga acgactcatt tgaaattatt gtctctcgtt gcacgatcat
      181 tgatgcgaaa agaggttaca gaacagttga gaaaagcaaa atcaaaaaca aaaatagcca
      241 caatattaac taaagaaata ggtgaagata tatgaagaag atgagtatag aaaagcagga
      301 acgtttgaag cgattgacgg atgaagctgg aattattagc gcgttagcaa tcgaccagcg
      361 tggtgctttg aagaaaatga tcaaccaata taaagaagct acatcagaag acatcatcca
      421 atttaaacag atggtttctc aatacctgac tccctatgcc tcagctattt tacttgatcc
      481 agaatacggc ctgcctgcag cagcgcttcg agagaaagaa tgtggcttgc tgctagctta
      541 tgaaaaaacc ggctatgata cgaaaatgcc gggaagatta cctgatttat tgaatatttg
      601 gtcggtgaaa aggttaaaag aagctggtgc ggatgcttgt aaatttttat tgtactatga
      661 cgtggatgaa ccagcagaga ttaatgtgca aaaacaaata gttatagaac ggattggttc
      721 agaatgcgaa gcagaaggca tacctttctt tttagagatc ctttcttatg atacaactat
      781 tggcgatccg tcttctcttg cctatgcaaa agtaaaaccc cataaagtta ttgaagccat
      841 gcgagaattt tcaaaagaac gttatcaagt ggatgtattg aaagtagaag tgccagtgaa
      901 tatgagattt gtggaaggat atgggcaaga gacttgttat agtcaagcag aggcatccgc
      961 gtatttctat gaacaatcga aagtgactga acaaccgttt atttttttaa gtgcgggagt
     1021 caatgctacg ctatttcaag atacattacg ttttgcaaaa caatcaggtt cgacattcaa
     1081 tggtatctta tgtgggcgag caacatgggc gaagggagtg gcaccttttg ttaccgaagg
     1141 cgaaaaaaaa gcgagtagtt ggatgttaga agaaggaaaa caaaatatta aggaattaag
     1201 tacattatta aaagaaacag caacacctgt ctacgttgat agataagaaa aagttgtttc
     1261 ttcataaaaa gatgctgctg caattagata gcgatcaatc gatgattcaa attaaacatt
     1321 cacttaaata aaaatatcga ccagatttct ttttcaaaaa tcttggtcga tatttttaat
     1381 tgctaaaact gttaatgaat ttttattaca ctcttattta ctatctactt ccgcttctgc
     1441 tgctttcctg cgttgcggcc tttgttgttt gtactgtttt ttggttgttt gatttgtttt
     1501 gttgcttgtt tgttgtcagc tggctttgct gattctgtca cgtcttttct tggagtgacg
     1561 acttgttttg gcgggttttg tttcaactct tctgccactt gtgctttcaa acgtggtttc
     1621 atcataacgt ttgtgatgaa tgtttgcaga cagctgaaca caccaccgac tacccagtaa
     1681 agtgtaacac cggctggcgc actgatcgac atgaagacga tcatagcagg agagacgatc
     1741 agcatcgtac gcatcgtctt tttctgttct tcaggaatac cgattgttga aatatatcct
     1801 tgaagcagat aagcgacccc tgcaatggca acaagcacca tacttggtga gcctagatcg
     1861 atgccgtaaa aagatgattc acggatacct tctgtgtagc gagccgtaaa atacaaggca
     1921 gagaagatcg gcatttggat caatagaggc agacagccga ttccgccggt catactcaat
     1981 ccgttttcgc gatatacttg ttgcatttct gcttgtgctg ccatttgttc ttcacgtgtt
     2041 gttgcagctt ttagcttttg ttgtgctgct tcaacttgtg gttttaacgc ttgcattttt
     2101 tcagattgga tcattgtttt tttcgattga ctgataccta gtggcaagat gatgatacga
     2161 acgatcacag tcattgcaat aactgcccag ccgtaagacc aattgaaatt ctcaactaga
     2221 tatgtaatcg cttggcccat aggatggacg agtactcgat aaacgattcc tgaagtgtca
     2281 gggtttccgt ttgaatccat ccgcacacag ccagataaaa agagcaggat actgacaagt
     2341 ccggatccca ataaccagtt tttccattta ttcatagaat acttcctctt ctataatttt
     2401 tacacaaact taactatact aaaaaagcgc atggaattca atcaacttca tagaaatttc
     2461 atataaatat tctttctttt tcgtagcgaa aaattttgaa aaaagacaaa aaatcaagaa
     2521 agaaatactt ggaaagaaaa gagtgattcc aagtgtttct ttcttattta aaaaactaaa
     2581 aactttttta ttctgcccga aatagaggag tcaaatgaac tcactgcaag tgatagatca
     2641 gcttttttac tacctgtctt taatacttga ggcaaagcgc ctcaaaaaca accttatcga
     2701 cggtgttcaa aagtcagttt cttggtatta agacaaattt atgcaaaaat gtggaaagcc
     2761 attttcacaa aattttcctt aatactcaaa actaagcgac tttttcgcaa ccagtcctta
     2821 atgcttgatg cagatcagct ttttcgcaac cttattgacg gtgttttatc agctgcctct
     2881 cggtattaag tcattacatt tcaaaatatg agaagctatt ttgaaaatgt ttctccttaa
     2941 tactcgaggc aaagcgcttc aaaaacaacc ttatcccacc acatcaaatt tctttcgttc
     3001 ctcaatcatc ggatcttctt ggacatccac atactgcact cgtccacttg ggctcggaga
     3061 cttcttgact tcttcgataa atttttggat gatgtcgtca tttccgattg cttcgattgt
     3121 gactgagccg tcgtcttcgt tttttaccgt tccggtcacg cctaactgat cagcaaccat
     3181 cttggtcata tagcgaaatc ccacgccctg gacacgtcct tggacattca ttttaacttt
     3241 gcgcatgttc gtcacctttc tttcctgttt tataaatcaa tgataccgat ttttttagga
     3301 tgaaacaagc tcaagcacct attccatgat ataatcagtt gaggtgagga atgtgaaaga
     3361 gatccaatca gcaaaaaatc caagaatcaa agaattaaaa aaactacata aaaagaaata
     3421 cagagaagaa aaggcagaat atcttttgga agggttccat ctgatcgaag aagctgcgtc
     3481 gtctgatgca gaaataaaag aactttttgt gacacagaga ggtatgaaag aatggggaga
     3541 ctggctggct gtaaacaaag agatttctac ttttctagtt tcagatgagg tcatgaccgc
     3601 tatgtcagat ctacctacac cgcaaggaat cttagcagtg atgacgttaa catctgaaga
     3661 tacgccagat ttttcaggag gctggctttt actagataat gtacaggatc ctggaaatgt
     3721 gggaacgatg atccgtacgg cagatgcgtt tggtatggct ggtgttttat taggtaaagg
     3781 aacggcagat atttatagta caaaggtgct gcgaagcatg cagggaagca attatcattt
     3841 gcctgtgtta caaaaagatt tggcagatgt aatccctgta atgaaaggca aagggtttac
     3901 ggtgtacggt acagaattaa atgaagaagc acatgcatta gacaaagtag aaaaaacaga
     3961 ggatttcgct ataatcatgg gaaacgaagg tcagggagta agtcaggaaa tcttgtcttt
     4021 gactgatcag aacgtatata tcccaatgaa aggaaatgcg gaatcattga atgtcggagt
     4081 tgcagcaggg atcgttcttt atcatttttc tttatgatta aggaactatt aagctttagc
     4141 tattttatct agagcgacta gaataatccg aacactggtg gtatacttag atcaatttca
     4201 gaagaaaatg aaattcaaaa aggtggcaga tcgataatta tgacagaaaa atggcgtgaa
     4261 gacgaagaat atatgtctta tgtacaagat cttttagaaa cagaagcagt gaaaaaatta
     4321 gcgaattaca cacagcatgt tcattccact cgtttggagc attccatcag tgtttcttac
     4381 tatagttatc tgctcgcaaa aaaatggggt ggcaatgcta aagcaacagc gcgtgctgga
     4441 cttttgcatg atctgttcta ctatgattgg cgtacgacga aatttgacga gggaacacat
     4501 gcgtatatcc atccaagaat tgctgtaaag aatgcagaaa aaatcaccga tttgtcggat
     4561 cttgagcgag atatcatttt gaagcacatg tggggtgcaa cgatcgcacc gcctaagtac
     4621 aaagaaggat atatcgtgac attcgtggac aaatactgcg cagtcaaaga agcggctcag
     4681 cccatgtctg caagtatgcg taagagatgg caacgttact ttgggaaaga atcctcgatt
     4741 taacaggtaa aaccaataat agaccatatt gagg
//
LOCUS       DACECR010000142         4734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.145, whole genome shotgun sequence.
ACCESSION   DACECR010000142 DACECR010000000
VERSION     DACECR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.145"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            25..696
                     /locus_tag="IWO17_002650"
     CDS             25..696
                     /locus_tag="IWO17_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0291495.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="IWO17_002651"
     CDS             755..1195
                     /locus_tag="IWO17_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291496.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="IWO17_002652"
     CDS             1207..1875
                     /locus_tag="IWO17_002652"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0291497.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="IWO17_002653"
     CDS             1939..2562
                     /locus_tag="IWO17_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ0291498.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="IWO17_002654"
     CDS             2618..3004
                     /locus_tag="IWO17_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ0291499.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="IWO17_002655"
     CDS             3217..4608
                     /locus_tag="IWO17_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ0291500.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1577 a          782 c         1007 g         1368 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaa
//
LOCUS       DACECR010000143         4710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.146, whole genome shotgun sequence.
ACCESSION   DACECR010000143 DACECR010000000
VERSION     DACECR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.146"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            106..357
                     /locus_tag="IWO17_002656"
                     /pseudo
     CDS             106..357
                     /locus_tag="IWO17_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="IWO17_002657"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="IWO17_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0291501.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1765
                     /locus_tag="IWO17_002658"
                     /pseudo
     CDS             1654..1765
                     /locus_tag="IWO17_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="IWO17_002659"
     CDS             1786..1869
                     /locus_tag="IWO17_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAQ0291502.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="IWO17_002660"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="IWO17_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0291503.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="IWO17_002661"
     CDS             complement(2994..3857)
                     /locus_tag="IWO17_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAQ0291504.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="IWO17_002662"
     CDS             complement(3859..4131)
                     /locus_tag="IWO17_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0291505.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="IWO17_002663"
     CDS             complement(4148..4363)
                     /locus_tag="IWO17_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAQ0291506.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4710)
                     /locus_tag="IWO17_002664"
     CDS             complement(4455..>4710)
                     /locus_tag="IWO17_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAQ0291507.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc
//
LOCUS       DACECR010000144         4648 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.147, whole genome shotgun sequence.
ACCESSION   DACECR010000144 DACECR010000000
VERSION     DACECR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.147"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..783
                     /locus_tag="IWO17_002665"
     CDS             <1..783
                     /locus_tag="IWO17_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0291508.1"
                     /translation="LSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDNY
                     GYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLTV
                     GTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIEA
                     RTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQQ
                     FVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
     gene            complement(854..1711)
                     /locus_tag="IWO17_002666"
     CDS             complement(854..1711)
                     /locus_tag="IWO17_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0291509.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            1922..4093
                     /locus_tag="IWO17_002667"
     CDS             1922..4093
                     /locus_tag="IWO17_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0291510.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            4161..4502
                     /locus_tag="IWO17_002668"
     CDS             4161..4502
                     /locus_tag="IWO17_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0291511.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
BASE COUNT         1521 a          861 c         1026 g         1240 t
ORIGIN      
        1 ttaagtatcc ctaccacaac accagaagaa acattggatc aggtatttga attttttgat
       61 cgttatgaat tgaagggaat cgggattggc tcattcggcc cgattggtat cgacaaaaca
      121 aaagataatt acggatacgt tttggcaact cccaaaaaag ggtgggcaca attcgatttt
      181 cttggcacaa tcaaggaacg ctacaatatt cctgctgctt ggacaacgga tgtcaatgct
      241 gctgcatatg gggaattgaa gcaaggagca gcacaaggaa aaaatagttg tatttacctt
      301 accgtgggaa caggaatcgg tgcaggtgtc gtgatcaatg aagaaatttt ttcagggatc
      361 gcacatcctg aaatgggaca tatttgggtg aaacgccatc cagatgacca ttatgaaggt
      421 acttgtcctt atcataaaga ttgtttagaa ggattagcag caggaccgtc tattgaggca
      481 cgcacaggga tcaaagggca agatctgccg caagatcatc ctgtttgggc tattcaggct
      541 tattatattg ctcaagcact gatcaactat acgttgacgt tagcacctga aaagattatt
      601 ttaggcggtg gggtaatgaa tcaggatcat ctccttcaaa aaatccgtca gcaatttgtt
      661 gaattgatgg gtggttatat ggaaacacct caagtaagtg aatatatcgt tcgttgggga
      721 ctgccgaatg aaagtgggat tatcggtagt ttgctgttag cagaacacga acatcaaaag
      781 taagcagaca aattacacaa aaagaacaga gtcttatcat tatctgctga taagactctg
      841 ttctttttta tttctattcc cataatccag gtaaagcttt accgactatt ttttcttgag
      901 taaatggatg gatcatgtgc agttctaatg catggagcat cagtcgttca gctgttctat
      961 tttggtagag agggtctcca acgatcggat gtcccttatg agctaaatga actctgatct
     1021 ggtgtgtacg tccggtttcc aaaacacaat agacttgtgt ttgttcagaa agtactttgt
     1081 tgacagtgac atgtgtcaag gcgctctgac catttttatc atctacgacc ctttttctcc
     1141 gatcatggcg atctcgtcca attttattgg tgatggtcat atctttggca atttctcccc
     1201 atacgatggc ttgatatctc cgatagatca ttttttgttc caacattctt ccaagtatcg
     1261 gcaagacaaa tggatttttt gcaaataaaa cggcaccgct tgtttctttg tccaagcggt
     1321 ggacaacgta aggtcgttgc tgtttagtgt cgagataagc agctaaatga ttgagtaagg
     1381 tactgttttc tgtcggttca tttggatgag tcttcattcc tgcaggctta tccactataa
     1441 tcaaatgctc atcttcatat aaaacacgga tgtgcttcgc atctcctaat tgaacttctt
     1501 gataattata atctgtttct tcgaataaca atgtgatctg gtcattgccc tttacttcat
     1561 gatggaacaa agcaggttgt tgattgatcc aaacgttttt acgtatacgc aaaaagtgtc
     1621 gaacctttct aggaactaac cattcctttt caagcaagtc gcgaattgtt gtttcaggat
     1681 ggttcatagg taaagtgatc gtgattttca caagtgtact cctttcagtg caatcgcaga
     1741 ctaattgtac catttttaaa acatatttaa gaaaaaactt acatttttct gttcattctt
     1801 atgttactct acttgcacta acgtaaaaaa acatattgaa ctacaactta agcaggaaga
     1861 aaacactctt tcactatggt aaaataagac gattgaatca aatagacagg agaagaagca
     1921 aatggatttt caaagcattt tagaaaagat aaaagctgct ttgatcagtt tttggcgttg
     1981 gatcagacca tatctcggac aattccatcg atggcgaaag cgcatctgga aaaaatatca
     2041 tgtgaacaaa ttgattttgc tgattggttt gatctgtgtc ttagtgacaa gtatctacct
     2101 ctttatttta gcaaaacaag caaatgtcga aacgttaaaa tcaggattaa gccaatcgac
     2161 agtcatttat gataagaaaa acgaagaagc tggcacactg tacgcccaaa aaggaacata
     2221 tgtagaatta aacgcgattt ctccctatat ccaagatgca gtaatctcga cagaagatag
     2281 gaatttttac gagcatcacg ggtttgatat caaagggatt gcccgtgcag cagtccgaat
     2341 ggtcttgtct ggcggaacat cagggggcgg aggaagtacg atcactcagc agcttgccaa
     2401 aaatgcgtat ctgacattag accaaacgtt tgatcgaaaa gcgaaggaat tatttttggc
     2461 aatcgagatc gaaaagaagt acagcaaaga agaaatcttg accatgtatc ttaacaatgc
     2521 ctattttggc aacggggtat ggggtgtcca agacgcttcc agaaagtatt ttggagtaga
     2581 tgcaacgaat gttacgctgt ctgaggctgc tactttggca ggaatgctga aaggtcctgg
     2641 tatttataat ccgatcgatc acccaaaaaa tgcgaatgat cggaaaaaca cagtactaag
     2701 tgtaatggaa gaaaacggaa aaatcacaaa agaacaagaa caagcagaaa gccagacaga
     2761 tatcagcgcg tatcttaatg acacatacga taattcagat tctggttatc gttatcctta
     2821 ttattttgat gcagtgatcg atgaagcagt taacgagtat ggtcttgatg aagaagatgt
     2881 catgaataaa ggctataaaa tctatacagc tttagatcag gattatcaaa agcaattaga
     2941 agctacttat caaaacagtg cattattccc agcaaatgct tcagatggtg ccatggtcca
     3001 atctgcttca gtagctttag atccgaatac aggtggtgta caagcactag taggacgaag
     3061 aggagatcat gtctttagag gattcagctt tgctacacag atgcgtcgtt caccaggatc
     3121 aacgatcaaa ccattaagcg tatatacacc agcactggaa gctggataca agccttccag
     3181 tatcttgaaa gacgaaccgc aaagttatta cgatgcgcat aattttgacg ggacatacca
     3241 aggagaagta ccgatgtatc aagctgttgc acaaagtttg aatttgccta ctgtatggct
     3301 tttgcatgag atcggtttac aaaaaggata tgataaagca gaagaattcg gcctgccttt
     3361 agcaaaatct gataagtatt atggcttagc ccttggtgga ctggaaaaag gagtatctcc
     3421 attgatcatg gcgggtgctt acagtgcatt tgccaatgat ggtcagatgt atacgccgca
     3481 tcttatcacg aaaatcgttg attcgactgg agcggtgatc gtcgataaca caaacaaaaa
     3541 accaaaacag gtcatatcaa aggaaacagc cgatgaaatg accagcatgc ttcttggtac
     3601 attttcaaat ggaacagcgg ctgctgcgaa tcctgcaggt tatacgatag cagggaaaac
     3661 aggaaccaca gaaactaatt tcgatgccac aaaagcaaat gaccaatgga tgatcggtta
     3721 cacgccagac gtagtgattt cgacatggat gggttacgaa gaatccagca agctgcacta
     3781 tttggaaggg acaagtggaa atgttgtcgg gaaagtcttc aaatcagcgg cagaaggcat
     3841 ccttccgtat acttcaaaaa caggatttag tgtagcagat gcctatgcca caggaggaca
     3901 agtggtagct gcagatcaag ttggaaacac aggaaattct aatggagagt ctggaaattg
     3961 gcaagataac ttgaatcaat atggtgaaca agcaaaagaa gggctgaaaa acttcggcga
     4021 tatggtgaaa gaaggcgttc agggaattgg tgaagcggct aaagacctct ggcggaaata
     4081 tcaaggagag tagccccttt gaaaacgaat aaccccatgt tacaatagag agtaactact
     4141 aaaagaaaag agggcataac atgtcaaaca tctacgatag cgcgaatcaa atcgaacgtg
     4201 agatccgtga attaccagaa ttcaaagcgt tgaaagaagc gtacgagaaa gtacaagcaa
     4261 acgaagaagc acacaaactg ttcaaagact tccaagcgat gcaaatcgaa ttacaagaaa
     4321 aacaaatgag tggacaagag ttcagcgacg aagatgcaat gaaagcacaa gaaatggctg
     4381 tgaaaatcca atcagaagaa gtaatcaatg acttgatgca aaaagagcaa ggcttcagta
     4441 caatcatcaa tgacttgaac cgtatcatca tgacaccagt tcgcgacctt tacagcgaat
     4501 aaaactattt tatgaaacaa cagagggtgt gaaagagacg ttcggccttg agcgatgaca
     4561 aaattgcttc ttattgccga taggctgtct cttgaacacc atttaccaag aggtcgtgag
     4621 cctaccgttt aactctgagt tacaagga
//
LOCUS       DACECR010000145         4590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.148, whole genome shotgun sequence.
ACCESSION   DACECR010000145 DACECR010000000
VERSION     DACECR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.148"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            148..1137
                     /locus_tag="IWO17_002669"
     CDS             148..1137
                     /locus_tag="IWO17_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0291512.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELIESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="IWO17_002670"
     CDS             1134..1553
                     /locus_tag="IWO17_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291513.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1562..1870
                     /locus_tag="IWO17_002671"
     CDS             1562..1870
                     /locus_tag="IWO17_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291514.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            1874..2236
                     /locus_tag="IWO17_002672"
     CDS             1874..2236
                     /locus_tag="IWO17_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291515.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2458..3864
                     /locus_tag="IWO17_002673"
     CDS             2458..3864
                     /locus_tag="IWO17_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ0291516.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1598 a          651 c         1042 g         1299 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc gaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgatcgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gctatcgtcg atttctgacg atcagcaaca cgaaaaatca gtgggtaatg agttgcgagc
     2101 tgatgaaaaa tcagcaccaa gcgaacggaa ttaccctaac gaatctgacg gaatattcgg
     2161 agggataccg aatagaaaaa gtcagatgag tgagagtgga tttgcgtcaa tgtttagtga
     2221 cgtaaattca cgataagcga atattttgca tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacggcga aaaccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgttactc tttgacaaag
     2401 agtaacagaa aggtttacac tctttttatt agcgacagcg cgaaggaggg gtttaacttg
     2461 gggtttagta tttcgttcaa aaagggaaca aagaatacaa gcatttctca taataatcgt
     2521 gagttaactg atagccaaaa aatcaatgac tggcataagc atattgattt cacaaaatcg
     2581 gatgaaaata tctatttgga acaaataaat attcgtgaaa aatatgaaga attattcggt
     2641 gaagccgtag aaaaatataa tgcaaagcag aaaagagccg atagaaaaat tgaggactat
     2701 ttagcaaagg ttcgcaagga taaaaaaatg gaaccacagc gagaatttat cgtgcaaatt
     2761 ggtactcttg atgattttcg tactactcgt gatgatggtt cttctactgg catatcggaa
     2821 caacaagcag aacaaaatcg agtgattgcc aacaaaattt tagttgagta tttcaaagaa
     2881 tttaaagaac ggaatcctag tttatccgtc tacaatgctg ttattcataa tgacgaagta
     2941 agtccgcacc tgcatttgaa tattgttcct gttgctgaag ggtataaacg gggtgttcaa
     3001 aagcaaccaa gttttaacaa agccttgcaa cagcagggtt tacgagcaga taaagagaat
     3061 ggtcgagtga tttggaataa ttttaggaat caggaagttg agtcaattga acgtttgatg
     3121 gcagattttg gttgggagcg tgagttagtc ggtactaaca aaatcaaaga tattcatgaa
     3181 tacaaagaaa tcatgagcga aatttccgaa ttgcgtcaaa cggccgtaaa agagcgtgag
     3241 agcgtttctg atgagttgag gtatatttgt aggcaaagag aagaaaacga gcgtgagaag
     3301 gctctggtgg cttctgagag agcgaagatt gaagaagaga agaaaaatat gctgaatgta
     3361 aaaaaagaga tggcggtcat tttccaaaaa gatatggttc ctttagaaag tttcaagagg
     3421 cttcctgatg gttcgtatca tttatctggg aatgattttg ttgatttgta tcaacgagct
     3481 acgagagcaa caaatgccaa gagaaatgct gataacatga gtgagcaata tttacagatg
     3541 cttgagcaaa atacagaatt aaatgagtat gtagctgcat tggaagaaga gcgtcatttg
     3601 tctggtagaa aacaattagc agatttacaa gtggaaaata aaaagcttaa agaagaaaat
     3661 cgctctttac gccagcgttt agagcgatta aaaaacgcat ttcaaaaatc gatcactcgt
     3721 ttgtctatcc gttttgggct tgcagaaaag gatatggggc tttctgagga attagaagta
     3781 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcataa attagagcgt
     3841 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
     3901 tgccgaggat aaggagttga cggctaaacc actagcctat taagctgaca tttgctaaaa
     3961 aatggccagt ccattttttg gccaacttag cgttagcgaa gtttgggggc gcccttttca
     4021 aagggtgcgg ttttatgttt ttggatattt gcatagctaa agagaagtgc tattattgag
     4081 ttatcaaata aataaactga aaaaagagca aaaaaataaa gtcgagcaac gctggacacg
     4141 ttctcgacct ttacggaaac taatcacact agctccgtaa ctcaaattca ttatacgaat
     4201 aatttcaggt ttcttcaaga ctagagattg ctggttatga attttttaga gttacgtgaa
     4261 ctctggaaaa ttctttccac caatctcttt tttctttgag gaggaactaa atttgagtaa
     4321 aaaaggcacg atcataaatg agtgggaaaa attaacagcc ccctctaaca ggcggttaaa
     4381 tgtaaaaact ttttacaccc ccttaaaaat gcagtggtcg attgatcgaa ttttatgtct
     4441 ttgggttttg gggttttaaa acgaaaaaaa gggggcgatt ttcgccctct tttttgatgt
     4501 tttttcttat cttgatacta taggcaacta tttctgcgaa gaaatcgggg ctatagtatg
     4561 ggtctatccc ttgtggctct aaggaagaac
//
LOCUS       DACECR010000146         4582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.149, whole genome shotgun sequence.
ACCESSION   DACECR010000146 DACECR010000000
VERSION     DACECR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.149"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..643)
                     /locus_tag="IWO17_002674"
                     /pseudo
     CDS             complement(1..643)
                     /locus_tag="IWO17_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(965..1186)
                     /locus_tag="IWO17_002675"
     CDS             complement(965..1186)
                     /locus_tag="IWO17_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291517.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(1284..1529)
                     /locus_tag="IWO17_002676"
     CDS             complement(1284..1529)
                     /locus_tag="IWO17_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291518.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1769..2383
                     /locus_tag="IWO17_002677"
                     /pseudo
     CDS             1769..2383
                     /locus_tag="IWO17_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2753..3754)
                     /locus_tag="IWO17_002678"
     CDS             complement(2753..3754)
                     /locus_tag="IWO17_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAQ0291519.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3913..4179)
                     /locus_tag="IWO17_002679"
     CDS             complement(3913..4179)
                     /locus_tag="IWO17_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0291520.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(4169..4525)
                     /locus_tag="IWO17_002680"
     CDS             complement(4169..4525)
                     /locus_tag="IWO17_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0291521.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1424 a          843 c          636 g         1679 t
ORIGIN      
        1 acaccgaaaa gccgaagagc gttccatttc ttcggttctt tttatatatt cctcgaatgg
       61 tctccatgcc cttaatcgtg gaagaggctg tacggagact ttgataaaat ttatttcgtc
      121 gtttaatagg tcgatggtct tgttctatta aattgttaag atacttcaca gttcggtgct
      181 ctgtcttagt atataaaccc acactctgta actttctaaa ggcggagcca agagaaggtg
      241 ctttatcggt cacaattgct ttcggctcac caaactgttt atggagtcgt tttaagaaag
      301 cataggctgc ttgcgtatcc cgtttctttc gtaaccagat atctaaggtt aagccgtccg
      361 catcaattgc acgataaaga taatgccaac gtcccttaat tttgatatag gtttcgtcca
      421 ttttccatga atagaaggat tgtctatttt tcttcttcca aagataatag aggactttgc
      481 tgtactctta tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata
      541 acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa
      601 taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt
      661 cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt
      721 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
      781 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      841 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      901 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      961 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
     1021 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
     1081 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
     1141 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
     1201 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
     1261 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
     1321 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
     1381 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
     1441 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
     1501 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
     1561 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
     1621 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
     1681 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1741 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1801 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1861 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1921 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1981 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     2041 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     2101 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     2161 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     2221 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     2281 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     2341 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     2401 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     2461 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     2521 tccttattta aaggcattta acattgacaa cttagtataa tatatcttat gtaaactaat
     2581 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     2641 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     2701 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2761 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2821 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2881 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2941 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     3001 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     3061 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     3121 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     3181 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     3241 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     3301 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     3361 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     3421 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     3481 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     3541 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     3601 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     3661 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3721 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3781 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3841 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3901 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3961 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     4021 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     4081 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     4141 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     4201 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     4261 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     4321 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     4381 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     4441 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     4501 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     4561 tatataacat aaaaattcga at
//
LOCUS       DACECR010000147         4501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.150, whole genome shotgun sequence.
ACCESSION   DACECR010000147 DACECR010000000
VERSION     DACECR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.150"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            161..391
                     /locus_tag="IWO17_002681"
     CDS             161..391
                     /locus_tag="IWO17_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ0291522.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="IWO17_002682"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="IWO17_002682"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0291523.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="IWO17_002683"
     CDS             1802..1978
                     /locus_tag="IWO17_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0291524.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="IWO17_002684"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="IWO17_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ0291525.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="IWO17_002685"
     CDS             2593..3060
                     /locus_tag="IWO17_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0291526.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="IWO17_002686"
     CDS             3145..4029
                     /locus_tag="IWO17_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0291527.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4146..4379
                     /locus_tag="IWO17_002687"
     CDS             4146..4379
                     /locus_tag="IWO17_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ0291528.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT         1534 a          685 c          985 g         1297 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 g
//
LOCUS       DACECR010000148         4433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.151, whole genome shotgun sequence.
ACCESSION   DACECR010000148 DACECR010000000
VERSION     DACECR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.151"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IWO17_002688"
     CDS             complement(543..2048)
                     /locus_tag="IWO17_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0291529.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IWO17_002689"
     CDS             complement(2045..2731)
                     /locus_tag="IWO17_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0291530.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWO17_002690"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWO17_002690"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ0291531.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1240 a          919 c          729 g         1545 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DACECR010000149         4425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.152, whole genome shotgun sequence.
ACCESSION   DACECR010000149 DACECR010000000
VERSION     DACECR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.152"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            690..1658
                     /locus_tag="IWO17_002691"
     CDS             690..1658
                     /locus_tag="IWO17_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0291532.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IWO17_002692"
     CDS             1669..2484
                     /locus_tag="IWO17_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAQ0291533.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IWO17_002693"
     CDS             2552..3262
                     /locus_tag="IWO17_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0291534.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="IWO17_002694"
     CDS             3298..>4425
                     /locus_tag="IWO17_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ0291535.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DACECR010000150         4416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.153, whole genome shotgun sequence.
ACCESSION   DACECR010000150 DACECR010000000
VERSION     DACECR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.153"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            59..913
                     /locus_tag="IWO17_002695"
     CDS             59..913
                     /locus_tag="IWO17_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0291536.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="IWO17_002696"
     CDS             1010..3259
                     /locus_tag="IWO17_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0291537.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4416
                     /locus_tag="IWO17_002697"
     CDS             3262..>4416
                     /locus_tag="IWO17_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0291538.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          625 c          806 g         1429 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actaga
//
LOCUS       DACECR010000151         4332 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.154, whole genome shotgun sequence.
ACCESSION   DACECR010000151 DACECR010000000
VERSION     DACECR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.154"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            63..296
                     /locus_tag="IWO17_002698"
     CDS             63..296
                     /locus_tag="IWO17_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291539.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(455..577)
                     /locus_tag="IWO17_002699"
     CDS             complement(455..577)
                     /locus_tag="IWO17_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ0291540.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            767..991
                     /locus_tag="IWO17_002700"
     CDS             767..991
                     /locus_tag="IWO17_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291541.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            1434..1640
                     /locus_tag="IWO17_002701"
     CDS             1434..1640
                     /locus_tag="IWO17_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0291542.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            1640..1891
                     /locus_tag="IWO17_002702"
     CDS             1640..1891
                     /locus_tag="IWO17_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291543.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            2072..2344
                     /locus_tag="IWO17_002703"
     CDS             2072..2344
                     /locus_tag="IWO17_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291544.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            2789..2968
                     /locus_tag="IWO17_002704"
     CDS             2789..2968
                     /locus_tag="IWO17_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291545.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            3027..3383
                     /locus_tag="IWO17_002705"
     CDS             3027..3383
                     /locus_tag="IWO17_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291546.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         1526 a          675 c          836 g         1295 t
ORIGIN      
        1 gatgatctac cgttttagtt tgagaaagaa ggagtaagca tggcaaaaaa attagtctga
       61 cgatggatct attgataccc gtaattgcat ttatagacgg gatttctttg ggcgtcactg
      121 aaaatccttt ggtaaataga attgcgaaag gatcactatt tcttgtattc gtgtatcttg
      181 tctgttctta tcttttaaaa agagcaggaa aggataattc cttttggcga ttgactaaag
      241 tcacagtagc agcgacagct gttatactta cggctattct accgctttat atgtagagtt
      301 aacgggaaat cgttgaactt cttgcgaaat attgctataa tgaacatgaa aaagaggagt
      361 tgcgctaaca acccctcatt aacagcaccg attaaagaac ggtggcagaa tattagttat
      421 ttggaaataa ccgctgacct gctaaagtag acggttattt ctttttgtca tttttaagca
      481 tatttacaat caaactaatc aaagcaatgg taaacatacc aaaaccaaga atggtttgaa
      541 ttgtctcata tgcggacaaa aggcttactc ctttcataga ttttagcact tacattcata
      601 agcaccacca cctttccaga agtcgccacc gtcttcaaac tttactgtct tgactattat
      661 agcatatcta aaatcgaata aaaattctca attttagctg tttttgctat aattttagcg
      721 aggtgatcgt gatggaacaa tatattggtt tactcgataa attagggtga tcaagacaca
      781 gccattgctt gtgcgattta cattacgcac gagtcatgaa ccaatcaatt gtgtagtggc
      841 caatatagaa atagcggata aattgctgat tttggaagaa ggcaagtata ctatttcagt
      901 gacaggtcac ttcaataaac gaaagcaact agtcattgaa tcaatgcaaa ttagaaaccc
      961 tgatcatttt acgcgatcga tgagcatata aaagaagagc tgcagtccta aatgaggatt
     1021 gtagctcttt ttgttttaca tagaaacagt aaaaatgaaa aggcctgttt aggaaggcac
     1081 aagcgccttc tcaattgaaa gacctttctc tcactccaac cgccaccgcc gttcgagaag
     1141 aaaggaagtg aacgctacgc gttcactaag ataaagggtc ttgcatagca agacaaaata
     1201 gaaaaaattt cctctcacca aaaggttcgc tcctgttcgt tatctcttta ttgatttgtc
     1261 agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg taaatggcac
     1321 gctgcgcgcc cttgtcttgt cgcttttcgt tgattcattc tggccaacaa atcaataaag
     1381 aaagaactaa cgaaaggaag taaaaccttt cctaagagga ggcagaatca atcatgcgaa
     1441 agaaatttga ttattggggt gttcccttca gcgaattatt cccaaactat catgcaccac
     1501 atactgtcga atgcgattgt ggagaaagag cgaaatgtat caaaagctat tgtttgtatc
     1561 aatgtccaac atgcgggaaa aaatatacct tgtcttatgg cgattatatt ttaattgatg
     1621 agaaaggaaa gaaacgctga tgaaaacaca acacaaatta ttgactgtaa aagaagtcgt
     1681 tcaaattttg gtggacaaag aagtcatttc tgaaaaagta gcagaagttt tacttattcc
     1741 gcggtactgg acgatagaaa cccctttagt agaggatgca ccgactatgc tatttgaaga
     1801 cctagtgaag attgcaggat atgaactgca ttatcaagaa aacttggaaa cttatgaaaa
     1861 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaaatatgg ctcaaaatta
     1921 ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg aggatatgac
     1981 gttcctatac aacgaaacac gtgttccaaa aaaacattat aaagaaaaat tgtcggtagc
     2041 tgtggaagaa atgattgaat aaggtgtcga aatgaatctt attgcgacgt actatcgaac
     2101 actggaagaa ctgaaaaagc aaaatgcaaa atggtttttc caagccttac tatgcttgga
     2161 agtaggtgtc aaaccaagca ctattaaacc ttctgaatac caagctttag aactaacgta
     2221 tgcgaaattt atcgaaacga aaaaagccaa gactgtttca agtgaatggt tagattattt
     2281 tgaaaatatc aataagtatg gcgcatatta caccatgaaa aaggaagaca acgaaaatga
     2341 ataaggtgat ttatcaacta acaacggatt ccatatcaac gatttgaaag aaaaaaaggt
     2401 caataatcat ttataccgaa aagtgagaat gtgtgaaaca aggacaaaaa aagcctagta
     2461 actaaggaaa tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg
     2521 tgcgaaataa acgggcaaag cccgataact taagagaaag aaggtgatcc atttgaacaa
     2581 gattattccc ttattgttag tactgtttgt tggcgaaata cttatgcgag atagtgcagc
     2641 tgtacagtta aacctcgctg tatttttggt aatactggtg ttttgtttga tcttttggcg
     2701 gtttgtatta gtcctcgtga gtctactatt caagatgatt gcagccatat ttttcatagg
     2761 gttatttcga tgaaaggagt gagtaccaat gccaattgca ataggaaata aacggttacc
     2821 cgtgacatta gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtatggcaa
     2881 aagtgaatct aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg
     2941 atttgatgta ccagcgctaa aaaagtgacg tcacttttta atttgatcta tataagtgat
     3001 atcactttta ccaaggaggg aattaaatgt taaaaactaa aaagaattcg tacaaattaa
     3061 agcttagtat actatctatt atttgtcttt taggcatgat aattagtggt catttgtata
     3121 gagtggcata tacgcagttt attgagtaca aggttcactc tttaggatta gctggggatc
     3181 gatacgttga atatgtccat tattcattaa aatgttcttc atatattctt ctttggctta
     3241 ttttttcatt gtttgaattg ctaaatttaa tagcaatatc ttcatatcac ttagataagc
     3301 taaataccaa atctagccgt tataacgaag tgtttaagga aaatgtgaag ttagaaagtg
     3361 acttaaacaa gctaaaaaaa taaaaagaag aaaacataag ttaactaaaa ttattttatt
     3421 aagaatgatg gaatataagg ataaggttac caacataacg atcattactt ctctgatatg
     3481 ctatggactt ctatcaatca gtattttgtc tatagattat ggagtaacta tactgcaagc
     3541 cttagttttg gctatgccta tgtatctatc tttgtttatc atgcatgaaa tatataagct
     3601 atttgtttta tcaaaaaatg tagaaatacg ggtaaatatt ttggtgaata gttatcaaaa
     3661 agaactcaaa gaaagaagac ggtggcggta tttattgaaa ggatgaaaaa gtaggatttg
     3721 acatactggt attcagaaag tgccgtcact tttaaaacaa tggaaaaggt ggagataaga
     3781 tgaagaaaaa acaaactaaa ctatcattag cagatattta acacagttaa ccgcaactga
     3841 agatggtgtt gttgaatttg aacaatctga aattgcggta gtagatgatc gttaccttaa
     3901 aatgccttac ttctttgacc aagcaaaggt catttgcctt tgtcactacg atggggtacg
     3961 tgattttagg cgttaaaaag tagtgattat atcaacttta cgagtaaata taaatttaga
     4021 catgaaactg aattatccaa tggaaaatca tggtttataa tctagaaaaa ttatagtaat
     4081 gattggatta tatatattct ttcgtatttt cgtacaactg tagcttgtca aaattgagaa
     4141 atttaaccga ttaggtactt tacatcgtta tttgggtaat ttctaaaaca tgaagcttac
     4201 caacgaataa actgagacta tcttattaat tgatcaatat aagaaaatat ctttgaggag
     4261 atgatgtact atgttttcgt ctatccacga ttggttaggt ggtcttttcg gcggtggagg
     4321 taaaggtttt ta
//
LOCUS       DACECR010000152         4224 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.155, whole genome shotgun sequence.
ACCESSION   DACECR010000152 DACECR010000000
VERSION     DACECR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.155"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(77..607)
                     /locus_tag="IWO17_002706"
     CDS             complement(77..607)
                     /locus_tag="IWO17_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ0291547.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            712..1263
                     /locus_tag="IWO17_002707"
     CDS             712..1263
                     /locus_tag="IWO17_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0291548.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1416..1889)
                     /locus_tag="IWO17_002708"
     CDS             complement(1416..1889)
                     /locus_tag="IWO17_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0291549.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2027..3499
                     /locus_tag="IWO17_002709"
     CDS             2027..3499
                     /locus_tag="IWO17_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0291550.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3496..4206
                     /locus_tag="IWO17_002710"
     CDS             3496..4206
                     /locus_tag="IWO17_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ0291551.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1323 a          758 c          819 g         1324 t
ORIGIN      
        1 atgaggaact atttttccgt ttctttctct aatactcagg tcagaacgct gatttcacaa
       61 cctctctttc acaaccttat tttactcgtt tataattttc atcgaagtat tttccgctac
      121 ggatatcatc tggcattcca tcgtatggtt cttcgctaat aacatcttca ttgtctatac
      181 ctgcatcacc catataagtg atcgtagcaa attcaggaac aagtaatttt ggataagttt
      241 catatttttc acgagctgct tccatttctg cgatatgttc gttttggaaa gtaacagtga
      301 tttctggatg ttcttcaacc catttaggga aaaagatcat tccgtgaagt ttcttttcat
      361 ttggcataaa gtccaatgcc acgtctgtcc ctgttggctc tgtccaagtg attttgaata
      421 cgccgtccgt caatttaacg atatcagctt cttggtcttt tacccaacgg cctttgacca
      481 ttccgccatg gatacggtaa tcaacagtat ggtcattttt ggcataccat tcatattccc
      541 aaccattgtc atacgtatag ataaagtgag ttccaataaa atcttccaat tctttaaatt
      601 ctttcatgtt taagcatctc cttataaatt aaaacatgta ataaactaca tgttttcgat
      661 tactcattta tgatagaatg gatttaaaga tttgtcaagg agggaaaact gatggcaaga
      721 aaaaatacac ttcgatatat tttgttaggg ttattaagta agaagaagat gacaggatat
      781 gaattgaatc aatcgttcaa aaatgaaata ggagaatttt ggcaagcaaa acatagtcag
      841 atttatcctg aacttgcaaa aatggaagag caaggaatca ttcagcatca agtagagatc
      901 acaggtgaaa aattggaaaa gaaagtttat gaattaacag aagaagggaa ggaacaattg
      961 gcagagtgga ttcatacacc caccaatgaa ctaccggtta atcgagacga atttgttctg
     1021 aaattgtatt tcgtcaaaga cgtgaatgat ccggcattag tagaaattat tcagcagcag
     1081 cgtgaccttc atgaagaaaa acgacttcat ctattagagc gaaaaaaaac gatctttcct
     1141 actgaaaaag aaaaacatga aaattacgga cactacctga ttttagcgca tgcgatcaat
     1201 cgggaaacag aatatgcaaa ttggctaaca gatgtattag aagaaagtaa ccgaaaaaaa
     1261 taaaaaaata tccgaacagt gatggagaat tcctttctat atgaaagaaa cgcagatcac
     1321 cgttcggata ttcttatgtt caaacaggcc ttttttcaag tatttatgta gcatctactt
     1381 attaaaaagc ttggtactta ggagattagt ggcgtttaga gttcttttaa caactgttcg
     1441 tagtcagctt tttcatcgac aggaaggagc ttgtaagtta attcgtctgt ctcgttgtca
     1501 tgaatgatgt acatcttagc attctcttct ttgaagttac cgatttgtaa aatcgtatag
     1561 cgttcgtcga tcaattcgat atctttgatt gcttttgaaa aaactttacg gacatggtgc
     1621 attttctttt ggcggatttc tgccttttct gcagcttttg acaaagtaaa tccgtcatct
     1681 aaatattttt tgatcagttc tacttttatg actgtaggta aacgatattt tctaggacaa
     1741 ttcgcatcag cttcgatcga acgaatgaat cccttttgtt cccaatacct taattgacgt
     1801 ggagacacac ctacgatatc acttaattca ctgatgccaa caaccaattg atcattttcc
     1861 aacaaacgtt taagggaagc cccaaccatt tttgtcactc cttttgtcct cttgcttccc
     1921 ttaattatat ccgtattaac ttatgtgtca agtaggttga caaatagcga atttaggact
     1981 aaagtattct agtagtttca gaaaaaattc agaaagaagg agaaatatga gtaacaacca
     2041 acccgtagat atttacggaa aaccatataa tcggacactt ttagttgttg tcttattaat
     2101 tggtacattt tgtaccgttt tgaatcagac cttattgacg acagcttttc ctacattgat
     2161 gaaagctttc gatatttcag cgtctgatgt gcaatggtta acgactggtt tcttgctagt
     2221 caacgggatc atgatcccaa taacagcatg gctgatcaat aaattcagtt caagaaattt
     2281 atatctttct gccatgacga tctttttgat cggaacgatt acttgcttta cagcacctaa
     2341 ttttagtaca ttactaatcg gtcgtttgat ccaagcttgc ggtgtgggag tatcgatgcc
     2401 attattgcaa aatatcatgc tatctatttt cccgccagaa aaaagaggat cagcaatggg
     2461 gatgtcaggg atcgttattg gacttgctcc tgctttaggc cctacattat ctggttatat
     2521 cattgataac taccattggc gtgacttgtt tggtatggta ctgccaatcg ttgttcttgt
     2581 cttggtttta gcattcttcc taatgaaaag tgttattcca ttgtcaaatc ctagcattga
     2641 tattttatcc gctattttat caacgattgg attcggtagt ttactttacg gattttctag
     2701 tgtaggagat gacggctggg gaagcttgaa agtaattggt ttcttagttg ttggtgtgat
     2761 tttcatcgcc ttgttctcat ggcgtcaatt acatttggaa catccattct tggaacttcg
     2821 tgtattcaaa tcatctacat tcacgattgc agctgtttta gctggtgtaa caaatatggc
     2881 gatggtaggg gcagaaatgg tcgtaccatt gtatattcaa aatattcgtg gagaatcagc
     2941 attccattct ggcttgatgt tgttgccggg tgcattgatc atgggggcta tgatgcctgt
     3001 gacgggtgcg attttcgata aatacggcgc gaaacgttta gccatcacag ggatgttcat
     3061 tttgacagcg gcaactgcac catttgcttt cttgacaaaa acgactccag ttttatatat
     3121 cgtgatcctt tatgcgatcc gtatgtttgg gatctccatg gtcatgatgc cggtaacgac
     3181 ttctggtatg aatgcattgc caaataactt gatcagtcat gggacagcgg taaataatac
     3241 attccgtcaa atcgcaagct cgattggtac agctattttg atcagtgtgt tgacgaatgt
     3301 gacaaaagca aatatcccaa gcaaatctgt attggataat acgccattag cttataaaga
     3361 tcaggcaatc aatgccacac tgtcaggtta ccatgccgcg tttatcgtag cgatcatctt
     3421 tgggattctt ggctttatta ttgccttttt attgaagaaa aaagaacctg ttctaatgaa
     3481 agcgggggat aatgcatgat tattggagtt ttgattgtca gcttgcttgc ttttgcttta
     3541 acgaacgtgt tcgctaaaaa aacatggcaa acctttttat ctttgatttt cggactaatc
     3601 ttcgtcgctt cactaagttt gatcgtcgct aatttgtcca atcactttgg aatggaaaaa
     3661 gtaacggaaa caaaaacaga aaaaattgtt tccagtgctg attcccaagg agcagatatg
     3721 cttttgtata aagctttagg gaatggaaaa gaaaaagtgt atctttaccg aacaaatgaa
     3781 aaacaagaaa aaccaaaagc aactggtacg gataacgaaa caaataaagt agaaaagaca
     3841 gacggcgatg ctgaaaaagt gacgaaaact acttattggg aatacaaaaa cgacatgtat
     3901 aaattctggt tcaatattgc tgataacaat cacgaatatg acagccgagt gaatacattc
     3961 aaaatcccag agacatgggt agaattaagt acggaccaag cagccaagtt agctgaatta
     4021 gtgaagaaac agcaaagtac gatggaaagt gaagcaaaag cttatgttca agacggtatg
     4081 gtaaaagcaa tgactgaaaa tccaaaaatg agcaaagcag agcaagaaca acgtacgaaa
     4141 gccttagctg ctgaattcca acaacaagca ttcgcaaaat tagtgaaaga agcaaaaggc
     4201 gaataatatt ttgaacacca tgta
//
LOCUS       DACECR010000153         4184 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.156, whole genome shotgun sequence.
ACCESSION   DACECR010000153 DACECR010000000
VERSION     DACECR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.156"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(2..1222)
                     /locus_tag="IWO17_002711"
     CDS             complement(2..1222)
                     /locus_tag="IWO17_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0291552.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1242..1847)
                     /locus_tag="IWO17_002712"
     CDS             complement(1242..1847)
                     /locus_tag="IWO17_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0291553.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1896..2828)
                     /locus_tag="IWO17_002713"
     CDS             complement(1896..2828)
                     /locus_tag="IWO17_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0291554.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3249..4109)
                     /locus_tag="IWO17_002714"
     CDS             complement(3249..4109)
                     /locus_tag="IWO17_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0291555.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1187 a          847 c          688 g         1462 t
ORIGIN      
        1 tttattttac agaagcactc gaaactgcat ctactgcagt ttcatcgtgt gtgctggcgc
       61 tggttactgc atctgttttt ccaccatgaa cagatgcact tgaaaccgca tcttcatttc
      121 caacaagttg ttgacccgtt tgttgtaaat accactcaat gattggtttg aaatcaagaa
      181 tgtattcatt gattccagca tactctccat tttcatcatg cattgcttgg taattatgca
      241 caatatattt ttctggcact tgttttggta catgaacacg taccgcatct gttgctccgc
      301 ttcgcagttg ttgaattacc cactctacat ttttatacga tttttctggg tgacagttgg
      361 ctaatggatt ccctacctga ccgggcacac gagcagctaa catctctttc gcttcttggc
      421 gcaaattata atataaaaat tggttattat tatctgcaaa agttaattcc atcggcatcg
      481 aatctaaaaa gtaattgatt tgatccactg tcaataaccc acgatttaat ttgacgtaag
      541 tatttccaga aacggcaccg actgcttcag aagctttttc caaccaatga tcatctgaca
      601 tatcaacatc gggtattgtc gtagcaattg ttcctgtcgc atgcaaatct tctggtggaa
      661 gctttttagg ccctgctaag gtagacacga ctttaatgaa ttttacaaaa cttgctaatg
      721 tactgcctaa aaagtcaaca gttcgtggat caatcaagcg atcttcttgg tcaaatgctt
      781 cttttgcttt tcccaataaa aattcatttc cgggcatcac taatgcattc acgcctggtg
      841 cgtccaaaat ttggcgcaag tgtaattgcg cgcgtgaagt tccttgatcg tagtaagaag
      901 cacccacaac cattacaggt ttattttcaa gtggatggat tttaaatgat agccattcaa
      961 gtgcactttt caatcctgct ggaaccgtat gattgtgttc tggcgtagca ataattaccc
     1021 catcagccat taagattttg cgatttagct gttgaatagc aggactattt gtttgatcat
     1081 catcttgatt gaacagaggt aaatctttga tttcaatgat ttctaaatta aatagttcat
     1141 gatattgttt ttggataaat gttaataatt tacggttata tgaaagttca gctgctgatc
     1201 cgacgatacc tattaatttc atctttgttt gctcccctcg attaaatcgt ttcccatgaa
     1261 aagctttcgg cttcacgttt atttgcttgg tgcgcatgtt gcaattgact agtgattgtg
     1321 acaaagagta agaaatcttt gaatagttca cgtaattttg cgacttgttc ttcatcaatg
     1381 agtcgtcctt gctcatcaaa ggcttggagt gaatgaccca ataaaaattc tgagcttggc
     1441 ataattcgtg cctttaactc tggtgaatcc aatatttggc gcaaatgtgc ttgtgcgcgt
     1501 gaagaaccta acgtgccata agacgctccg gtaatcatca ctggtttatc cacaaacgga
     1561 aaaataccat atgaaagcca atttaatgcg ttaatcaatg aagcaggaac cgcatggtca
     1621 tactctggtg tgctgatgat tacgccatct gcttgttcaa tttttccagc aatttcttta
     1681 acaacatttg gtagttctgt gttttcaggt ttgttaaata gtggcagctc cttgatttcg
     1741 acaatttcga tttctgcctc atttacaaaa taatttttca taaactgtaa taattcacgg
     1801 tttgttgacc gcgttgaatt tgttcctact aatccaataa atttcatctt cattctcttt
     1861 cctttctaac atcgatttct gactgttcgc acgtactaaa ttttttcaaa tccttttgta
     1921 tacaagaatt gattctcttt tgttacaact gctccctcga tacccggctg ttcttcaagc
     1981 gctttcaaca cttctaaaga agatgctcca aataaacgtg tagtccagat ttctccatct
     2041 acagaagaat cagacaaaat cgtcaggctt gctacgtcat ttttaactgg aaagccagtt
     2101 ttcggatcaa tgatgtgatg atacactgac gaaccatttt gtagataacg ttcgtagatt
     2161 ccagaggtaa ctaaagattg gttgtttagt ttaaccaccg caagcgactc ttcacgtgaa
     2221 cctttaggat ttcgcaaacc aatacgccaa gcaccatccg aacgtcttgg tgattcacca
     2281 aaaacaacga tatttcctcc taagttaact aaagcaaacg ctgcatttcg ctctcggcaa
     2341 tgtgtaatta atttatctgc aatgtaacct tttgctaacg cccccaagtc aatcatcatt
     2401 cctggtttgg ttaaaaaaac ggttttcatt ttttcatcta attgaatttg gtgtggatcc
     2461 gttagcagaa gtaactcagt tatctctgct tttgttggaa ccaatgcatc ttcaaatcca
     2521 atgtgccatt tttgtacaac cggaccaata gcaatattca gataactatt tcttgcacaa
     2581 ctatgtgctt ttcctaattt gatgagctca aaaagttcag gatgtacaat cacagcttgt
     2641 cgtccagcag cttgattgat ggccattaat tcagaagttg catcatgtgc gctgaagcga
     2701 tgttcataaa cgtgcaacca ttcgcctaac tctgctagta atcgactagg ctcttcatgc
     2761 ccaacttgta atgtaattgt tgtccccatc atttgccaga ttttcgtttc ttcgatcacc
     2821 tgattcattt tgtaccttct ttacttgaac tacggattac atgagtaatt agtttttatt
     2881 tttgttactg ttttcacaac cagatgatac gcctgctaga tttatttgtc aaccgatttt
     2941 atatattttt ttatataata aggtaatttt aatataaaat tttatattgt aaaaaaacaa
     3001 ataagggaaa caaacaacac atttttgtgt gttgcttatt tccctctaac tatcgttttc
     3061 tatttgatgg ttaacctact taaaataaat actgcaacga caaacggaga tcaagcatgc
     3121 tttgtaacgt attttacgat aattacgact ttattttatt actctatctt gctgccatac
     3181 ccgtctatcc ctaatcatct ttggtgaatt ttttcaaagt tagcataaca aaaataagta
     3241 ttttttcctt agttaatcaa tagtatcatt tttttcatat gatttaattc atcaattaat
     3301 tttttgtttt tattaatttt agcaaaagct agtgcatctt gaatcaattc gagcacttct
     3361 gccttcgact tatttttagc ctgtgcgttc ttcgctaatt gggcagcagc tcgtgctaag
     3421 taataagtca aatggttatc cgaacaaata tcgattgcgt aatttagtag tgcatcactt
     3481 gtttccaaat caccgtcttg cgcaaaaaaa acaccacaat gaaagacgat atttaacacc
     3541 cgccagatat cttctgttgt acgaatcgga tacgtataaa ttttttcaaa cactcgattg
     3601 aaataaactt ctgctttttc caactctttt tctctagcat agaccattcc aatacctgta
     3661 tatgccaata aacgaaacaa cgtgttggct gtttctgcca tcaacaactg atcaaaatgg
     3721 aacaacacat ccgtaattgg tgcattcgta aaaatcattt caaatccttt tagatagtag
     3781 taacgtgcaa tcagctcaga ctcgtcaatt accttgggat caatggctgc taaaatctgt
     3841 gtcaattgtt catattcact cgtaatcagt gaaaactctg ctttttctag ctgttcatca
     3901 agttgtgtat tgatcacgtt catcgaagaa aaaagttcaa ctaacggaag attgattcga
     3961 ttacataatt tgattaaaat ttttaacgaa gggacttgac cattgttctc aaatttactt
     4021 aatgttgctt gtgtacaaat tccttcagac acttcttttt gagataagtt ggcagccttt
     4081 cttgccgtaa taaatttttc aatgttcatt attttctctc caatcagacc tcacacgaaa
     4141 atcatttctg tctcgatttt agcatgtttt ctttgggaat gctt
//
LOCUS       DACECR010000154         4063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.158, whole genome shotgun sequence.
ACCESSION   DACECR010000154 DACECR010000000
VERSION     DACECR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.158"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            581..1270
                     /locus_tag="IWO17_002715"
     CDS             581..1270
                     /locus_tag="IWO17_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0291556.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1257..2465
                     /locus_tag="IWO17_002716"
     CDS             1257..2465
                     /locus_tag="IWO17_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0291557.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2759..4030
                     /gene="serS"
                     /locus_tag="IWO17_002717"
     CDS             2759..4030
                     /gene="serS"
                     /locus_tag="IWO17_002717"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ0291558.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1319 a          702 c          808 g         1234 t
ORIGIN      
        1 ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac aagtaattgt
       61 caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc aactcattta
      121 tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc gatagaatca
      181 agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat ttccaacatg
      241 tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac aaataataat
      301 atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca tttctagctt
      361 acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc gagagacgta
      421 aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc aactagatgt
      481 ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg cttttttttg
      541 ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt tgattgctga
      601 cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg ggtatgaagc
      661 agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc ctgatattga
      721 attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag tgaaagagct
      781 acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg atatggataa
      841 gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta atccgttaga
      901 agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg ccccagatca
      961 gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg aagttaaaac
     1021 aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct acttgcttgc
     1081 tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt ggaaacaaga
     1141 aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag ataagattga
     1201 agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct ataaaattga
     1261 cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact tcaaaagaaa
     1321 tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta ttaaatgttt
     1381 ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg aatgcgattt
     1441 gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg aacggccgga
     1501 attttattat tccctttttc tttctattag atattggtgt tttgtattgg cgcttgattc
     1561 gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac atcgcaaacg
     1621 gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga gtggtaacaa
     1681 gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt aaaatcgaaa
     1741 aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca ctcacgtcca
     1801 tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa gatcttttaa
     1861 agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg gacgacttat
     1921 ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc tttgacatga
     1981 tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa aaaaatatcc
     2041 aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc gaaaaacttg
     2101 tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga gcaacgaaga
     2161 tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa aataacggag
     2221 caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt gtagaagaat
     2281 ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa agcattgtgg
     2341 ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct ttcatcatgc
     2401 accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt attgacgaaa
     2461 gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca cagaacccag
     2521 tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag gataatttgg
     2581 aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa cagccgttat
     2641 gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc agactgattc
     2701 gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag gaaatcatat
     2761 gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat tgatgactcg
     2821 gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc gtcgtaagct
     2881 actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg agatcgccca
     2941 attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag aagtcggtgg
     3001 caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga aacaaattgc
     3061 aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg aagaggacaa
     3121 tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa aaccacactg
     3181 ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag tagccggtag
     3241 ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct acaacttcat
     3301 gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt atatcgtcaa
     3361 cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg ttttccaatt
     3421 ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta actattataa
     3481 tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa gccctgcttt
     3541 tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc ttcaccaatt
     3601 caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag aattggaaaa
     3661 aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc gtgtgatggc
     3721 cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag aagtatggat
     3781 acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag atttccaagc
     3841 acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata ctcatactct
     3901 gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa actaccaaaa
     3961 tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga atctgaaagt
     4021 tattaaataa agaggctgtg aaaaagccgc ttagtttcga gta
//
LOCUS       DACECR010000155         4041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.159, whole genome shotgun sequence.
ACCESSION   DACECR010000155 DACECR010000000
VERSION     DACECR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.159"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            179..775
                     /locus_tag="IWO17_002718"
     CDS             179..775
                     /locus_tag="IWO17_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0291559.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IWO17_002719"
     CDS             899..2698
                     /locus_tag="IWO17_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0291560.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IWO17_002720"
     CDS             complement(2976..3188)
                     /locus_tag="IWO17_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291561.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1188 a          689 c          712 g         1452 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat a
//
LOCUS       DACECR010000156         3923 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.160, whole genome shotgun sequence.
ACCESSION   DACECR010000156 DACECR010000000
VERSION     DACECR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.160"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(573..1130)
                     /locus_tag="IWO17_002721"
     CDS             complement(573..1130)
                     /locus_tag="IWO17_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291562.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IWO17_002722"
     CDS             complement(1309..1920)
                     /locus_tag="IWO17_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291563.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IWO17_002723"
     CDS             complement(2033..2290)
                     /locus_tag="IWO17_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291564.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="IWO17_002724"
     CDS             complement(2887..3165)
                     /locus_tag="IWO17_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291565.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="IWO17_002725"
     CDS             complement(3199..3450)
                     /locus_tag="IWO17_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291566.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DACECR010000157         3664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.161, whole genome shotgun sequence.
ACCESSION   DACECR010000157 DACECR010000000
VERSION     DACECR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.161"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..277
                     /locus_tag="IWO17_002726"
     CDS             <1..277
                     /locus_tag="IWO17_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAQ0291567.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="IWO17_002727"
     CDS             420..641
                     /locus_tag="IWO17_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0291568.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="IWO17_002728"
     CDS             657..1499
                     /locus_tag="IWO17_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291569.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="IWO17_002729"
     CDS             1562..3364
                     /locus_tag="IWO17_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAQ0291570.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3423..>3664)
                     /locus_tag="IWO17_002730"
     CDS             complement(3423..>3664)
                     /locus_tag="IWO17_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0291571.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1307 a          519 c          746 g         1092 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
     3481 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
     3541 gagttgaaat tttgacaacc taatttttct aaggaagaaa aaacagtccc aactgttttt
     3601 ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt tttacgtggc
     3661 ggaa
//
LOCUS       DACECR010000158         3644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.162, whole genome shotgun sequence.
ACCESSION   DACECR010000158 DACECR010000000
VERSION     DACECR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.162"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            154..417
                     /locus_tag="IWO17_002731"
     CDS             154..417
                     /locus_tag="IWO17_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0291572.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..689
                     /locus_tag="IWO17_002732"
     CDS             417..689
                     /locus_tag="IWO17_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0291573.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            766..1095
                     /locus_tag="IWO17_002733"
     CDS             766..1095
                     /locus_tag="IWO17_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0291574.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1085..1372
                     /locus_tag="IWO17_002734"
     CDS             1085..1372
                     /locus_tag="IWO17_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0291575.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            1656..2513
                     /locus_tag="IWO17_002735"
     CDS             1656..2513
                     /locus_tag="IWO17_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0291576.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            2514..3062
                     /locus_tag="IWO17_002736"
     CDS             2514..3062
                     /locus_tag="IWO17_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291577.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            3322..3644
                     /locus_tag="IWO17_002737"
                     /pseudo
     CDS             3322..3644
                     /locus_tag="IWO17_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1376 a          469 c          739 g         1060 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac gcattacgag tcacagtaat tacttgtaaa tacattatct cgtggtataa
      721 tgtatttact aagtaattac atactgttag aaatgaggga tttaaatgat tacaaaaaca
      781 agaaaacaag gcaattcaat catgttgaca gtacctaaag attttaatgt tcctaatgga
      841 gtcgaagttg aagcaaagtt ggtagagaat ggtattcttt atgaatttgt tgaaccacaa
      901 aaagaatttt atgattttag cgaagatatt ttgtctgata taattgcaga aggctacgat
      961 aaagatgaga ttcttgtaga atttaagaat agaaaaaaca aaatgcattc ttcctttaga
     1021 gatattgctg aagatacact tactaattct aaagtaatga ctaaagagga gttggcaaca
     1081 gaaattggct tatgaaatcg ttccttcaaa acatgtcatt aagtatttaa aaaagctaaa
     1141 agagaagcca ttgaaagaaa aatttttgaa tttaatttat gatgaaattg cgattgatcc
     1201 atataagggt gaacaaaaaa ctggggattt atcaggtatt tggtctagtg ggttcaaata
     1261 tgcgggcaca acatatagag tagcttacga gattatagaa aacaaggtta ttccagtatt
     1321 gttatgtgga acgcatgaaa acttttatga acagttgaaa aaattaatct agtgatttgt
     1381 tgtcgttctt aagaaggacg gcttcttttt atcgaaaatc tgattcgtct acatgactaa
     1441 agtcgcgagt attctctgct tctcgataaa ttaaataacg tcattacttt gaggaaagct
     1501 ataaatcaat atatttcagc ttttccttat ttatgtacta ccttttactt attaattata
     1561 aggtagtaca tatatatctt tttttggtag taactaaaaa taaatatgtt ataatagtat
     1621 caaatgaata ttgctaaaag aaaagaggta ggaacgtggt agcgaagaaa tatgtaatcg
     1681 gtaattttaa aggtggcgta ggtaaaagta cgtgcgcgca aatgttaggg tttgaatcag
     1741 cagttaatga aggaaaaaga accttgataa ttgacttgga tatgcaagga aatacttctg
     1801 atgtgatgaa cttaacgcat atgaacttca ctaacgaaga aggaggcgga gaaggtgaac
     1861 cgattgttta cgaaaatact gtgactgacg tgttgataaa caatcttcca cctaaagagg
     1921 ctgtctataa agtaatcaat aacctctata tacttccagc agacatgtcc tttgaaatgt
     1981 atgatgattg gatcaaagac caatataatg attcattatc ccaattcaaa tatatgaaag
     2041 aaaagctaga accattattt gatgagtttg acgtcatcta tttagatgtc ccaccttcta
     2101 tttctgtata tagtaaatcc gcgatgtaca ttgcggattg ggcaatcgtc gtcttacaga
     2161 cccaagtaaa atcgatgaga aatgcgatgc aatatcttga gtatatggac ttttttgtga
     2221 aagaatttga tacaaattta tatgttgctg gtgttattcc tttcatgttg gaaagcggtg
     2281 acgcagtaga tcaggaaatg tataaacaag cccaagagat ttatggggaa catttgatta
     2341 aaaatgttgt actcaaaaat gcgcgcttaa aacgatacga cggatcaggt attacgatgg
     2401 agaagacaaa gaaaggtctc ttaaaacaat gggatcgcaa aagccacgaa ttatttatca
     2461 gtattttgaa agaattggaa gaacacgaga attggtatgc ggaggtggag taattggctg
     2521 acatcaaagg acttgatatt tttgggaaca acaaaggact cgccaaaata aaaaaagaag
     2581 cagaagcaaa gaaaaagcag caagcaatta caccatctga aaataagcaa ttagaagtgc
     2641 accaagttca agaaataaaa acttcctcca atgttgaaag acagagtaga ggaagaacaa
     2701 aaaaagttgg cgcaccaatc agaaagtttg ataaggttta ttccaccaag catcctttaa
     2761 aactgtcgac tttgttaaat gcgacttcca gagttttagt agaaaagtat gagactaata
     2821 tgacaagaga tgaattgctt agaaaagctt tagatgagta tattaaacag aatcttacgc
     2881 aagaagataa gcaagatttg tttaatgacg tggtgaaaga actagacttg ttcagagaaa
     2941 aatatccaat tgttccagaa ttagatgatc gtggagaaat aataagaact accagtcaga
     3001 taaaacaaga taccgaagac tcactgcgca aagggtgggg aatgaataat aaagttagat
     3061 aataatcaaa gaagaactaa gtaactacct ttaaacggta gttacttagt tcttctttga
     3121 tttgcatgaa atagagggga tttatttttc tggttacgtg attaaaaaat ccaaaaaatg
     3181 agcataggaa cggttatgat tttagggtgt gttttaatga gatattgtga tttcattaac
     3241 aagtgtatga taatctgttg ttgagttagt aaataagtaa tagagctaac tctaaaataa
     3301 aactattagg aggagatgta ggtgaaaaag tttaaaaaga aaatatattt aggattggtt
     3361 gttggggtga gtatgtttgg gttcatggct catactaaac aagtaactgc agatactaac
     3421 caaaaagaaa ggattatgag ccaattagat gactctcaaa taagtatgta cccaatgagc
     3481 taattgtcag tttaaaggct acacaaagaa tttcaacaga gaaaaataaa ccgcaaaatt
     3541 tattgggtgt aaaagttatg gtggaaaaag atttgactct taggacaaat gaaagcgatt
     3601 gtaataagcg agatcaaggg ttctgttgca aagttttcca aaaa
//
LOCUS       DACECR010000159         3597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.163, whole genome shotgun sequence.
ACCESSION   DACECR010000159 DACECR010000000
VERSION     DACECR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.163"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(35..1336)
                     /locus_tag="IWO17_002738"
     CDS             complement(35..1336)
                     /locus_tag="IWO17_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0291578.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1565..3112)
                     /locus_tag="IWO17_002739"
     CDS             complement(1565..3112)
                     /locus_tag="IWO17_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0291579.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(3214..3567)
                     /gene="tnpB"
                     /locus_tag="IWO17_002740"
     CDS             complement(3214..3567)
                     /gene="tnpB"
                     /locus_tag="IWO17_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ0291580.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT         1027 a          711 c          676 g         1183 t
ORIGIN      
        1 ataagaacat ctaaggtaga attccttttc tactttagat gttttttgat tcataggtaa
       61 tcagattatt tagtaagaaa attctggtct tcattttccg aaagcttcga aagccataag
      121 cgactcgttt caatgctttg atgtgggtat gcagattttc tattttggca tttgaataag
      181 gcaattcgag ggctaaacga atgccggatt catagtttat aaaagctttt tttatagaat
      241 gcagttcttt tggaacggaa ttgggcatcg ttcttacaag tttaaagaaa gtgtccgcct
      301 ctttgtcgtg aaacgcttga agaatttctt gatagtaaaa ataggcgatt cgtaattcgt
      361 cagagatcga aagcagacga tccaccactt cactttcggt gagatacgcc caaccaaaat
      421 tgcgccattt ggtccatttg tcataaacaa gcttgtcctg tcgtttgatc agaagccgag
      481 acaacgcctt taattgtcga taatatttgg cttgtgttgg atcgtttcga tcaaagcttt
      541 tcatgattcg tacgcgaata tcctcaaatt tacgaatgag atgtttgaca atgtgaaaac
      601 gatccacaac gagttttgcc tttggaaaac attcctttat cagagaggca taagaaacat
      661 tcatgtccgt cacgatgacc tttaccttca atcgtgcgcg tcgttcaaat tgcataaaat
      721 aagaaacgag agcctttttc ttccgtgaag gaagaatatc cagtaatttt ccagtatcgc
      781 catccataca agaaaaggag tagggaccga cttgattttt cagtgaacga aattcatcca
      841 ttaataaaca acgcggtaac caatttcttt ttacttttgt catggggatc agagaagcga
      901 gtgtccgaga aacggtcgac caagaaacgc cgcaatgctt agcaattgtc gtcattgctt
      961 gtttttcagt tagctcttct aaaattttat agcgcacttg tttagcgata aagcagtttc
     1021 tatcgacaat gtaggtattc gccgtaaaat aggtgtggca gccacgacag tggtagcgtt
     1081 gtttcgctaa agcaagataa gtcagttggt tcgcacattt attaagttga atgagcgatt
     1141 ttttctttcc gttcttaacg atcatgcctt catgattcat ctgtttacac aaaggacagc
     1201 gacgaatttc tttacttaac gtcgcagaaa caacaagact cttgatattc ttaatgctct
     1261 tcgattccac atttgtgatg aaaatgtttt tatctttgat tccaagtagt agtttgatat
     1321 gatgaagatc aggcatacag aacgctcctt tgattgtgtt gtggtgattc aattctaacg
     1381 gagcttctgt atgttttgtt ttttcatgct caaaagggac cgatagaatg attttctatc
     1441 agtcctagaa attatagagc cttttttatt tggtcttgaa gaagaaaagt aagcgcccca
     1501 tagaacagta cctgtacgtc aaataacccg acctatagaa tagaatcggg aatgtttggc
     1561 gtttttagtt acgagaaaaa tgttgttgta catccttgtt ccaaggcaac agttgatcta
     1621 aagtttctgc tgaaagtacg ggaagatttg gtatcttatc aaatagaaac tctagatact
     1681 ttacaggatt caatccgtta agttctgctg ttttatatac actcagaatg attcctgaag
     1741 ttcgtgctcc ttcaagactt gtggaatgca accaattttt ccgtcctatc accagctctt
     1801 tgatatttcg ctccgcacga ttatttgtga gttctaaacg accatcttca aaaataggtt
     1861 caaaaccact tcgtagttta cacgcatatt gaacggctgc atccaacttg gattttgcaa
     1921 caggatctat cgttgccatc cactcataaa aagcaataaa taatggacgc agttcttgtt
     1981 gccgacagct taatcgcttc tcaggagaaa agtctttcca tttcttttct aggacaaacc
     2041 atttatccaa ttgtttgact gcttgagctg cgggaatatc cgtgtttttc tttccttttg
     2101 gaattgcttc gaaaaatttc cgccgaacat gcgcgaaaca atagacaagt cgtacacttt
     2161 ccacggcatt atacccgcta tagccgtcac agtgtaaata ccctgtaaac tccttgagaa
     2221 aagttttcgg aacatctcca gcacgacttt cgtcgtggtg ataataaacg atgggatgct
     2281 gactgtcttt tcctgtacaa aactgccagt aataggtatc tgttttggca ctttcaatca
     2341 cacgataact tgtttcatcc ccgtgtagaa tctcttcttg attaagatag actcttaatc
     2401 gttctcctaa agggtctaac gcgttttgca ctgctttaat atgccaattg gtgatattat
     2461 ctctcgaaat atccaaccca tgtgtttgtt tccaatagtt ctcttgccga taggcaggaa
     2521 ctttctgacc aaatttcatc cggatcgttt ccgccactaa actattggaa cctaagctat
     2581 tagaaatcaa aggtttagga acgatcgctt ttttaaaaga agaaagtcct tctttttcgc
     2641 agcttttaca acaataggta tgttgataat gggtgagaca atccaacata gcctgatgga
     2701 aacgaatttc ttctcgtact tttgtccgtc cgatatcttt catctgtgaa ccgcaatgtt
     2761 cacaaaattg ttcttccaaa ggaaggagat gttctttctc tgttataggt aagtgtttga
     2821 ttttgtctgc tttcgttcct acaactcgtt gatgtccttt tactggaata atggtggcag
     2881 ctgcttctgg aacacttaca gaaatttctt catcactaaa aagattcaat tgcccattcg
     2941 tttcctcaaa gagagtctct ttagagcgtc caaataattt gttggttaaa taattgacct
     3001 tttcattcaa tagttggatt tcagttgtaa gattctgaat ggtttgttgt tgtgcttgat
     3061 tggttttatg ggcttcttga agcaactgaa gcaacgtatc tgtagtttcc aaaatttttc
     3121 accaccttga atcgaactga ttttgttaca gaataccaca agaaacgcac aagaaatagg
     3181 ggttcaacac attttttgtg ttaacccccc agcttaaaaa actccttttt ttcctggcaa
     3241 aatagctcga ggttgatcga tacttaatcc ttctaacaac catcgaacct gttggttcgt
     3301 taaggttcga acttcgtctt tcgtccgagg ccattttaac ttgccgtttt ctatccgctt
     3361 ataacagaga agaaaaccat caccgtccca atagagaagt ttgaaacggt cttgacggtt
     3421 cccgcaaaat aaaaaaacgg catcttcgta aagctcgagt tgatactctt gttgaataag
     3481 actggcaaga ccatcaattc cttttcgcaa atctgttttt ccgcacacaa tataaatatg
     3541 tttggtttga agtaaatcat aaagcattgg acaacagttc tttcaaaata gcttgaa
//
LOCUS       DACECR010000160         3591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.164, whole genome shotgun sequence.
ACCESSION   DACECR010000160 DACECR010000000
VERSION     DACECR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.164"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(194..991)
                     /locus_tag="IWO17_002741"
     CDS             complement(194..991)
                     /locus_tag="IWO17_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ0291581.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(988..2172)
                     /locus_tag="IWO17_002742"
     CDS             complement(988..2172)
                     /locus_tag="IWO17_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0291582.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2197..3444)
                     /locus_tag="IWO17_002743"
     CDS             complement(2197..3444)
                     /locus_tag="IWO17_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ0291583.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
BASE COUNT          959 a          772 c          694 g         1166 t
ORIGIN      
        1 caagaggttg tgaaaaagct gttctgcatc aagtaataag aagagccaag taaaaacagt
       61 ctcttatgtt tttaacttgg ctggacttat caccgtagat gtaagctttt gaacgccgtt
      121 tatttgcaac aaaaggggct gtgacacgat ttttgtcaca gccctttttt tgcgaacatt
      181 catttagcaa tttttagaaa cttctgccgc caccgccaaa agtcccgcca ccgctagaat
      241 gggtagtact tccgccaccg ccgcttccag gaggtgggct tttggggatt cttcttgtcg
      301 tgacaaaaga atttgtcaga cgatcggttt tttcagttaa tttgatactg gatttttctc
      361 ggaatggata tttgtagatg cctgatttca attgataacg cgaaacagtc acaaaataaa
      421 agacaagact aaggataaga gccaaaacaa cagcaatgat gatttctact gtcgtcaaaa
      481 ccttatagcg tgtaattttg ccagtttctt cgtctacacg ataatgtcct cctggaactc
      541 cgtcttccac atatgttgct gatttagaca ggtaagcttt tgcagcattg taataggcag
      601 catttgtcat ctgatcataa acatcatcta agatcgaatc gattctgcta tcagtcaaat
      661 aatcgatcat attaccagaa gtcgaaatgt agatttcacg ctgtcccata tctaatagca
      721 atactgagcc attttgatca tttccgactg cgtctcttaa gtaattatca gcaaagtctc
      781 ttggttcttc tgaattagat gtagtcgtta cgatcaagac tcgtcctttg attttttcat
      841 tcagcttttc tgcctgttgt tccaaagatt ggatttgatc tgttgtgaat aacccagcct
      901 catcatttac cgtagctgta tctgcatgag tagccaccgg ccagccaaca cttactagca
      961 aaagaaagaa taaaaaaaat tttttcatca tatcaagtaa cctccgatca gaaagaggat
     1021 cgctagccca gcgaaaatcc caagagcggt cagtcccaat tttttctggc tgatcggcag
     1081 gacaccgctg acctttcctg tttgtccatt cattgcgtag tagtagactt ttttacttga
     1141 gtcattgctt cgataagtaa caagccatac cggcagtaat acgtaattat ttacttcgtt
     1201 tgtgattgaa gcagaagttc gcacaccggt caatgttgta tatccatttg cggtatcttt
     1261 taacagcttc tctgaatagt cttttaattc agattgaatc tgtgatttga ttgcctcata
     1321 ttcgatatct cttttttctg cttggaaccc ggctaaatac tggcttttaa aatcaattgc
     1381 tttatctaac gggaaaggtt gcacgccttc gaccatcttt tgttgcgtat tttttgataa
     1441 ggcatttttt actagttctt taaaagaaag cttgcctgct cttgaaacag caaattgctt
     1501 cgtttctgtg tattcgatgt cacctatccg ccatatgcgg ataactgtac cattcgcctg
     1561 catggcacca tctacttcag catctgtagc ccaataaggg aaatacacac ctgtcaactt
     1621 atcgatttga tccttattga aaaaagcttt gggaacaaac cattttttct tggtccaagc
     1681 gagaaatctg ttgattgctt cttctttctc gacagcaaaa ggaagtactt tatttggcaa
     1741 gaattctccg cttaaacgtc ccgatagtac gacgggatta tggcagtagt aacaataagt
     1801 cgcagcagtt gtcgcatcag tcacgatctc tgcaccacag ttgggacaaa gaaaaagatc
     1861 tatcgctgcg gtattatctg cagattcgtc cgctgtatct gaaaaaccgc ctgcctcgtc
     1921 aaccgctttg cgctcggctt cattcatatc ggcaagctgt tcttgggctg taaaagtaaa
     1981 atcttcggta ggtgaaggtt cttctttgct ttcttcgcca gccatacgcg cttctttttg
     2041 cttttgctca tattggctga cttcttcttc tgtatagata ttcaggcagt attcgcaatg
     2101 aaatttttgg tctttgggat caaaagtcaa cggaccgccg caattaggac atttatgtgt
     2161 aagaacatcc atcagctgca cacctttcta aataaattaa ttaacgggga ttcctttaaa
     2221 cggtttacca cagtttggac agaatttagg gatgccgttg gaaagatcca cgatctcatt
     2281 gcattcactg catcgcattt gtagcttagg ctgaacgttt tctacaggtt tggcagctcc
     2341 gcagttggaa cagaattttc cggaattttc tgtcccgcaa actggacatg tccaagtatc
     2401 cgcatttcct tgtgcagttt gttgattttg tttttctgct tgttgtttga tttgttcttg
     2461 gttattttga gctgcttgag aaagataaga accatttgca ttcattccca tacctacacc
     2521 cataaagccg gtcattgctc ctgcttcatt ttttccggct gcttccatac cacgtgcaat
     2581 ggaaccttgc acgtatcctt cacgtacgct aggatcacct agcatcgccc cttcgttacg
     2641 catattgatc agtttcacag aatcatcggt gtaagaaatg ctggctacag ccacagaaac
     2701 aatctccatc ccacgtaatt cacgccaaga atcatccaat gcagtatcca tgtatttgct
     2761 taattccaaa ctcttagatg gtacataaga aatccgttga ccatctgcag acatctgatt
     2821 gattgctgat tgtagcgctg ttaggaattc agctaaatac tgctcattga tatcattgat
     2881 ttccacttgt gttttgtttt tagggattgc gtttgtataa aataaaatcg ggtcaacgac
     2941 atgaatagaa taagtgccat gtgcccgtaa gaataattct gcgttataga aattgtcaaa
     3001 gtagttcaac ggcgcgcttg taccgaattt gattcccttt atttcttgga gattgatata
     3061 aaaaacttgt tgtttctgcg gtgtgacgcc gccaaatttg aatcggtcaa aagtttcagc
     3121 aattgcttct ttcagtgttc cattgaacat ggaaggagct gcgtcatttt taatggtgta
     3181 ataaccttcc tcagcggtat aatcgatgat ttttccacca tcgaccaaaa gcatcatcat
     3241 gtttggatag acatggacga ctgttccgtc tgttaaaaca tcttctgttc ctttacgatt
     3301 ggagccacgt ttgtcatctt tgcggacttt gacacctttt gtcatgaccg ttgtatcact
     3361 catattgtct ggttcgatga cttctagcca ttgatctgcc agaccgccgc caaccatact
     3421 tgttgctgct ttgataagac ccataaaaaa ttcctcctct gattcagcta gtgccaaaca
     3481 attattgttt atttattata ccataggaag atgtgaaact tctcggacta gagacggaat
     3541 tgaaatggaa aattgcgtat tgtttaaata cctgaaaaag ccgagaagga t
//
LOCUS       DACECR010000161         3509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.165, whole genome shotgun sequence.
ACCESSION   DACECR010000161 DACECR010000000
VERSION     DACECR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.165"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..272)
                     /locus_tag="IWO17_002744"
     CDS             complement(<1..272)
                     /locus_tag="IWO17_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HAQ0291584.1"
                     /translation="MNTPQIFNFGQQEVRTVLLNDEPYFVGKDVAEILGYERADNAVR
                     NHVDEEDRLMHRISASGQNRNMTIINESGLYSLILKSKLPSAKKFKR"
     gene            complement(398..619)
                     /locus_tag="IWO17_002745"
     CDS             complement(398..619)
                     /locus_tag="IWO17_002745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291585.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPREHQKNSFATG
                     EKKTQKRSEKVLRIIQFLLEKIRHFGNLF"
     gene            917..1264
                     /locus_tag="IWO17_002746"
     CDS             917..1264
                     /locus_tag="IWO17_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0291586.1"
                     /translation="MTTFERVKMLADKRKISIVELEEKLNFSKNSLYAWKKSKPSIDK
                     LNAVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREIL
                     SAMIEAYLEKKDN"
     gene            1300..1695
                     /locus_tag="IWO17_002747"
     CDS             1300..1695
                     /locus_tag="IWO17_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0291587.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            1749..2060
                     /locus_tag="IWO17_002748"
     CDS             1749..2060
                     /locus_tag="IWO17_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291588.1"
                     /translation="MIYTEFKEWLEKNTTGYETFIVKATNYQIEKNKNRPPKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            2072..3220
                     /locus_tag="IWO17_002749"
     CDS             2072..3220
                     /locus_tag="IWO17_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0291589.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
BASE COUNT         1356 a          526 c          564 g         1063 t
ORIGIN      
        1 cgtttgaatt ttttggcaga aggaagtttt gattttaaga ttaaactgta aaggcctgac
       61 tcgttgatga ttgtcatatt acgattttga cctgatgcac taattcggtg cattagccta
      121 tcctcttcgt caacatgatt tctgacagcg ttgtctgcac gttcgtatcc aagaatctca
      181 gctacatctt tacctacaaa atacggttca tcatttaata aaactgttct tacttcctgt
      241 tgtccgaaat taaaaatttg tggtgtgttc attttgctca ttcctttctt tggtataatt
      301 ttgaatagaa agcgaggtga aaatagtatg gaagaattta atatggatgt cgacgcctta
      361 ttcaaacaaa ccgtattcaa gactgttaat aaagaattca aaatagattt ccaaaatgac
      421 gaatcttttc caacagaaat tgaattattc gaagaacttt ctcagagcgt ttctgagtct
      481 ttttctcgcc agttgcaaaa gaattttttt gatgctctcg tggatgaatt tcatcaacaa
      541 cagcactaaa cttttggtct ttaaaattta aagttattaa agctcctttt gcggaaggag
      601 tttttttatt ttgtttcatg atgtttcctc cttttcttta aatatgtaag ctaacaaaat
      661 tagctaattt tgttgacagt ttctacaaaa ttttgtagaa taagtgcata gttaaataag
      721 cacagaatta ccctataaat taacattcta agtttcccga ccttgaattt gtttacttta
      781 ttaggtatct ttcttattgc ttgttagctt attaaattag cttacgaaca tatattacta
      841 taaagttttg tagatgtcaa cgattatcta caaagttttt aagatgtgtt ttaggcatga
      901 tcggagaatc attattatga caacatttga gagagtaaaa atgttagcag ataaacgcaa
      961 aatatctatt gtcgaattgg aagaaaaact taattttagt aaaaattcac tttatgcgtg
     1021 gaaaaagagt aagccatcca ttgataaact aaatgcagtt gctgactatt tccatgtttc
     1081 aacagattat ttgctaggac gcacagatga tcctaacgcg ggagttgcac cagaggaaag
     1141 aaaactaacc gtggaagaag ctttagcatc tgttatgagt agcgacggaa aaccgctcac
     1201 cgataatgat agggaaatac tatcagctat gattgaagca tatttagaga aaaaagataa
     1261 ctaaataagt aggtgagtca tttggacagt caaattgaaa tgataattaa tgaactcggc
     1321 gttaaggtag aagagcgtga aaaccttgat gccgatggcc attatgttgc ttgtatgaat
     1381 accatagtaa taaaagctaa tttatctaag tatagaagac aaagaacctt attacatgaa
     1441 ttaggacacg cttctaaaca tcatgataat tattttttat ataacttagc attctctctc
     1501 cattcaaaaa tggaatatga ggctgatcgc ttcatgattg aaaaattatt agatagatat
     1561 attgcaaagt ctgaattaga accacacaat atcaattaca tgaaatttat agaagataac
     1621 aatttaagcg ttcgcttcga accacttgtg aaagaattat taaaagctcg catctattgt
     1681 tatgcagctc tctaaaattt tttaagcaaa aaaagaacat atgttcaaaa atagaaaggt
     1741 gaacaaaaat gatatataca gaattcaaag aatggttaga aaaaaacaca accggatacg
     1801 aaacatttat cgtcaaagct actaattatc aaattgaaaa aaacaaaaat agacccccaa
     1861 aaaaacgctg ggatgataag aaaatagata aagctgtatt agaaatgtgg aaacaagtcg
     1921 tgactaactt gtatcaaaca attcgtaaag aaaaaggagt tccattaatt aacgggaagg
     1981 aaatatggct tgaatttata gaggaacaag gactgatcga atttttcaat gatagcatgg
     2041 cagaattaga atttgaatag gggtaatatt gatggcaatg ataaaacaat ataaaaagaa
     2101 aaatggcgaa aaagcatggt actttaaaac ttatctcggt attgatccgc taactggaaa
     2161 gaaaaaatat actactaaac gaggatttag aacacaaaaa gaagcaaaaa cagcactttc
     2221 taggttagaa ctagaattac aaaaaacagg aatgcccaca agtacaaata ctactttcaa
     2281 agaagcagca gaattatggc tagaaagcta caaaaaaact gtaaaagaaa gttcatattc
     2341 aaggactaaa ataatcttta ataaacatat atatcccaaa tttggaaata ttaagctttc
     2401 taaaattaat acggcatatt gtcaaaaggt agtaaatgat tggagtgaaa aaggaacttc
     2461 aaagcagtac cctcttttca taaactatat gaataaagtt tttaagtatg ctataaatat
     2521 tggtttaaca tctgataatc caacattaaa tttacttatt ccaaagccac aaattaaaac
     2581 agaaaagaaa ttaaaattat atacaaaaga acagttggaa ttatttctaa atgaagtatc
     2641 tcaagaacag aatccatatt ttaaaaacag agactatacg ctctttagac tattagcatt
     2701 cagcggatgt agaatcggcg aaatattagc actcacttgg gacaatatta attttaaaac
     2761 aaatgaaatg gccattaaaa aaactgtagc tcgttcagat aaatattata tatctgaaac
     2821 tcctaaaaca aaaaaatcaa atcgaataat ttatttagat gaaaaaacta taaagcaact
     2881 aaaattttgg aagctcgaac aaagaaagta cttatttcaa ttaggattta ctaaagctaa
     2941 ttatttgttt accaatgacg aaaataattt cacaattaat cagtcagtgg cagaaagata
     3001 caatatatat cgtgagcgtg ccggcttacc ttatatcggt ctgcatggtt ttagacatac
     3061 acatgcatca atgctatatg aggcaggcgc agatcacaaa gaagtccaag aaagaatggg
     3121 ccacgcaaat ataaaaacta ctatggacac atatacacac attactaaca gcaaaaaaga
     3181 agaaacaaca caaaaactaa caaattatat taacttctaa gaaagtatgg tcaaaagtat
     3241 ggtcaaaaaa atcccttact tagcaaaaaa agctccatcc cttgccacac aagagataga
     3301 gcagtttatt tattaagctt cttgagctac tggataaaca gacacttcaa ttatactgtt
     3361 tcctgataag ctctcaatgc ttctgaaccc atgatattag tgcttttctt ctattattaa
     3421 atatcttgta attttctatt ctttctaaaa agtatggtca aagtatggtc actattgata
     3481 gaatattaat agtagtagaa agctgatat
//
LOCUS       DACECR010000162         3344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.166, whole genome shotgun sequence.
ACCESSION   DACECR010000162 DACECR010000000
VERSION     DACECR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.166"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(112..732)
                     /locus_tag="IWO17_002750"
     CDS             complement(112..732)
                     /locus_tag="IWO17_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0291590.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(734..1603)
                     /locus_tag="IWO17_002751"
     CDS             complement(734..1603)
                     /locus_tag="IWO17_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ0291591.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(1626..2033)
                     /locus_tag="IWO17_002752"
     CDS             complement(1626..2033)
                     /locus_tag="IWO17_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0291592.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(2052..3341)
                     /locus_tag="IWO17_002753"
     CDS             complement(2052..3341)
                     /locus_tag="IWO17_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0291593.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT          929 a          736 c          586 g         1093 t
ORIGIN      
        1 aagctgtttt tttgaaattg ctccttgtga ctcggagctg aacgacaggc tcacgacctc
       61 tcggtaaaca gcgttcaaaa gccaattttt cgatattaag ccatttcttt gttacttatt
      121 gtcattgatt tctttttcta atttatccaa tagtggagtg taatccactt gagtatagtc
      181 tgttacatca aattcatata gtgttcctaa ctggaatttg tcatattttc ggtcggcgat
      241 gatctttttg aatgcttctt tcgtaatatg attggtccct ttcgttcgat cttccaatac
      301 gtcaccatag tgataatgat ccatgatata gctcaaatga cgatctgatt cacgatcacg
      361 tttgtaccga cgttgccata acacttcaaa gtctgctgtt aggcgaatcg tgatcacctg
      421 atatccgtat ctatctgcta attcttggag tcgtgctttt tgcttatcac taaaaggata
      481 ctcggacaca atcactttct ttcctgcttc catatacaaa tcaagtgctt tgtaatagaa
      541 aggataaact ttcgtccttt cttgttccgc ccgttcttcc aaattgttaa acccgatgga
      601 ttctgcatag tattccttga tttcatcgag cgtgatggca tacatatctg gaaatcgttc
      661 ccgtagttta ttcataagat atgtttttcc tgtaccagga ctaccagcca gaagaatgaa
      721 atactttttc atctcattta tttcctaagt taattggact ttccaagttc ataaacagtc
      781 caatcttatg cttgacaatt tcttttactg cttcatttgc tggaatcaca ttatggcgaa
      841 gagacttgtt gatttctgtt tcactaaagg tttctttagc tactttcgtc cagttgacta
      901 aaagctccgt tccaacattg aatttgcgga tcccgttatt gatcaattca ggataatctt
      961 cttctttcac acctgttcct ccatggataa ctagggggac atcaactact gcatcgattt
     1021 ctttcagcaa gggaatgttt acttctgttt ttgatttgaa ttgtccatga ttcgtaccga
     1081 tagcaatagc taatgcatcc acgcctgtag cttctacaaa tttcactgcg tcttccggtc
     1141 ttgtatatac cttgtcattt tcagcgacat ggattccttc ttctgtaccg ccgattgttc
     1201 caagttctcc ttctacggaa acaccgtgcg catgggcata ttctactact gcttttgtct
     1261 tcaggatatt ttctttgaaa ggcagatggg acccatcata catgacagaa gtatatccgc
     1321 tgtcgattgc ttctttgata tcatcaaagt ctcgggcgtg gtctaagtgc aagactgcat
     1381 caaccatttc ttcttctgca atagctgtca ttacattgac taacacatgc atgccgatat
     1441 atttagcggt atcgacactt gtttgaataa tgatcggcga acccatttct tttgctgcgc
     1501 ctagcatagc aggaagcatt tctaggttat gcgtattaaa agctccaact gtaaaattca
     1561 actcttctgc tgtttttgtt acttctttca atgttgtata catcttttat tgccacctta
     1621 tctttttatt tgtttccttt gatcgttact tgttttgcaa tttgtcgcat ctcatccatt
     1681 ttgcccatgt agtaatcgat cccttgatca tcaccagcac gagcagcatc tgcacgcttc
     1741 tggttataga ggctcatcag ttttttatac ccatctccga tcgtctgttt attttccaaa
     1801 ctcttttcta aatattggat ggcttgatcg ttcacataca attcagaaaa tttaagtcca
     1861 acttgttcat aaagtttcgc taatttatgc ttatcttggg tagtggaaat ctcttgtttc
     1921 aattgtgtaa tctctgattg aatcttttcg gtcgcttctg gcgatagggc tttttcttct
     1981 tttgccagaa cttttttcgg ttctttttct tttttcttaa aaaatccaaa catcattttc
     2041 cctactttca attaaaatgc tttcaaaact gggcttaaga tccaggcata taagagtgct
     2101 gcggctactg tatctacatc ggttgctgta gcattgttga atcccatcga attaaagatc
     2161 gttactagca aagcgggcaa taatgtaata aagaaaccat gagcaatacc gccaatgatc
     2221 gctcctcggc gtccgccaac tgcgttgcca aatatccctg ctgtccctcc ggcaaagaaa
     2281 ttggttagca taccaggtaa aatcatcgct aagccgaaca tgggcaaagt aaacatggca
     2341 ataatcgtac caatcgttgt cgtaatgaaa cctaaaatga cagcatttgg gctataagga
     2401 aagaaaactg gacaatctaa ggcagggatc gcatcaggaa ctaatttcat agctatccct
     2461 cgaaatgccg gcacgatttc acctaacagt agacgtacac cagctagcaa tacatacaca
     2521 cccactacga attggatggc ttgtaaaaag gcaaacatga tatagttttg atctcctgat
     2581 aattcagaag cgaatggttc gcctgcaaat aaaacagtga tgatatatag cgggaccatc
     2641 acgaccatga cagacaaata agtatcttgc agaaattcga aatgtgtcgg caacttgata
     2701 tcttcgatcg actttttatt ttcgcctttc tcaccaaaaa gcttagcaac accggcttca
     2761 aataagtacc cgatcgtaca aaaatgacct aaagcaatat catttgatcc agtgatttta
     2821 cgaatgatcg gttgcgctac tgccggcata gcaatggcga aacaagctcc gataaaacca
     2881 ccgaccaaga tcaatacgat ccctcgcaga ccggcaaaat agccaaagac tgtcgtcatc
     2941 gttgccatcc ataaaattgc ttgaccagtt aaaaaaatgt acttccattt tgtgaaacga
     3001 gccaaaataa tattgaatac aaaaatggct aagaacgtca gtgcaatatc tctacctaaa
     3061 cccaaatcat tcattgcttg accattgata gattcaatag aagggatgat cccttgcata
     3121 tgaaatcctt cagtaaagat tttcccgaaa taagttaaac ttccaacgat gatgctcgaa
     3181 ccggcactca acacttgaaa tcctaggagt gtctttaacg tgccagagat gacttgtcct
     3241 gctgatttct tctgcagtag tagacccaac atagcaatca atgcaatggt gatcgatgcc
     3301 tgagtcaaaa tattttcgat gataaaattg ataacgccca tgtt
//
LOCUS       DACECR010000163         3133 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.167, whole genome shotgun sequence.
ACCESSION   DACECR010000163 DACECR010000000
VERSION     DACECR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.167"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            208..1041
                     /locus_tag="IWO17_002754"
     CDS             208..1041
                     /locus_tag="IWO17_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ0291594.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1143..2843)
                     /locus_tag="IWO17_002755"
     CDS             complement(1143..2843)
                     /locus_tag="IWO17_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291595.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
BASE COUNT         1110 a          520 c          623 g          880 t
ORIGIN      
        1 gaagcagcag tttgaatact gtttagtgga gaggtcgtga aaaagctgcc ctgcaccaag
       61 tgataaaaac agccaaacaa aaaatagctc ctggtccatt gagcttatta ctgaggagtt
      121 agcaggagaa cactatttgc tggagaggtt gtgacaaaaa ttttgtcaca acccttttgt
      181 ttcgaaatga agacaaggag aaaaccaatg aaattcatga cactcaacac gcatagctgg
      241 cttgagccag aaccagagaa aaaattgcaa gaattagcag ataagatcct gttagaagat
      301 tatgaagtga ttgctttaca agaaatcaac caattactgg aatctgaaga agtagaacct
      361 gcgaagctaa tgaagttctg tccggtaaag aatcaagtac ctatccgtaa agataatttt
      421 gcctaccgtt tagttcagct tttaaaggaa cacggaaaag agtatttttg gagttgggaa
      481 atgagccaca tcggttatga taaatatgaa gaaggaaatg cactgcttac gaaaaaaaca
      541 ttggaaagtc aggtccttac cgtctcccaa tttcaaaaga aagaagatta tcagacacgc
      601 aagatcttga ttgggaaaac aaagatagat gatcaggaca tttttgtctc gagctgccac
      661 ttttcttggt ggacagataa aaaaagtggc ttttattttg agtggaaaaa cttggaaaac
      721 tactttttgg aaactcgcgt accacttttt ttcctaggtg atttcaataa tccagtagac
      781 tctcaaggtt attatactgt aagagaaagt tgccttcttc ttcaagattc gtatgttgtc
      841 gcgaatgaaa aaggaaaagc agctactgtg gaaaagaaaa tagatggttg ggaacaaaat
      901 acagaaaaat tgcgtataga ttttattttc gtccctgaag gaatgcaggt taaaaaatat
      961 caacggattt ttgatgggat cgatagtcca atcatcagtg atcactacgg ggtggaaatc
     1021 gaattagatg tgaacgaata aaaaatataa catataacat aaaaaaactg attaaaataa
     1081 aactctccaa acgattatac cgtttggaga gttttttgat cggatgtatt tatccctttt
     1141 gcctaagcac gtaaaagctt gtatctatga taaataaata aacatagcaa ggtaaaccag
     1201 ccaatcaaag taagagaaag aatgaaatag aaatagattg gcgtttgata atgaagcgtg
     1261 agctcgttga ttcctttttg ctgcttcacg attggggtcc cgatatctgt tacttcataa
     1321 tcagtcaatg tttttccatt ttgctgtagc acagtccgat cgtagacgat aacagggata
     1381 tgaacagttt tgtccccagt gtttttccac ttgattgtca aaagaccgcc tggatgtgtt
     1441 tttataaaag ttgtttcgtt ttctagtatt tcttcgttat accggtcgta tttattgtct
     1501 tttgtctcct tatagattgg cagatagtca ggtgtggttt tttggaaaac gaacaagctg
     1561 gtagacatat ctttgctgaa gaatgttttc cttgcttctt ccggcgtatc gaatagataa
     1621 gtatgtcgac tgacaaatgt ttcattttcc cacttttcca gatgctggta taagttctgg
     1681 atcgtctgtg ctgaaaaaat aagaatgcca aatatactta aaagacggaa ccacttttgt
     1741 tcagaccact ggttcagtga aagggcaagg taaagtagaa gcaatagcgt aaatggcacg
     1801 aaaaatcgaa atggaaactg gatcaaacta ataaaaggca catttttttc gatcaatgtc
     1861 gtccatggaa tcaagctagt agaaagaaaa aagaaaacaa gacttgtgta caacacgatt
     1921 ttttggaata aggtgttctt ttgttgaaag aaaataaaaa gcaaaaaacc tgataatatc
     1981 aatataggaa aggccttagg gtaatggagc caataactgc catccagatc tatcgccttt
     2041 tgactcattt ctctgttaac aaagggagga agaatcttat tcccactgta aacaatagct
     2101 aatcctgccc aaatatttgc tgtcagaaca aaataaagca agaccgactt aatcagttga
     2161 agcagtgctt ttcctttttc agtctggtta aaaaagtaat aaagataaaa agggatatac
     2221 atcaaagcta acatgatcga agacagtaaa tggatctgtg tcataagtcc cactgagaca
     2281 gcgactttga taacagggaa ctttttatga aggacgaaat cagcaatagg gatcatacat
     2341 aaaggataaa aacaagtccc ccagctagtg aatccttggt tgatagacca gtattgaata
     2401 gaatacgtac tcatgaataa aacggctact ggtacactta cccaatttcg aacattgaca
     2461 tatctcagta aaatcagcat agaaaatcct gaaataagat aaagaatgaa atttgaaaga
     2521 atctgataat gaaaccagct acctgcaagc aacacgatca atccttgtaa ataagcgaaa
     2581 aatgggccgt aaacggcatt cacgatcctg ccactttgct ggaatccata aagggaagga
     2641 aaataatgga aatccccata tttaatttgt tgcgcggcat catagaaccg attgtaatgg
     2701 aatggggcat cagcacccaa aattagttta tgtgtgaata aatgaggcat gagaaatgcc
     2761 aaagcaatga aaaaaattgc taaaaatggg ccatattttt tcaattgtcg aactcctttg
     2821 caaactttag ataaattcat catatatcct atgcgagagg taggcaagaa attagaaaat
     2881 tcatgaaaat atttatgaat tttacaagaa aaacataagt gaaagttcta aggagagagg
     2941 atgcgacata agttttgttg catctccttg ttttgaaaca gaatttttct taatactcga
     3001 aacaaagtag ctttttcatg acctctcggt aaacggtgtt caatcagctg cttctcggta
     3061 ataagctcac tagcctagaa aatatgagaa gctattttct gactagcgct cttattactc
     3121 aaagcataac gca
//
LOCUS       DACECR010000164         3057 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.168, whole genome shotgun sequence.
ACCESSION   DACECR010000164 DACECR010000000
VERSION     DACECR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.168"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            422..739
                     /locus_tag="IWO17_002756"
     CDS             422..739
                     /locus_tag="IWO17_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291596.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(830..1195)
                     /locus_tag="IWO17_002757"
     CDS             complement(830..1195)
                     /locus_tag="IWO17_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291597.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1403..1885
                     /locus_tag="IWO17_002758"
     CDS             1403..1885
                     /locus_tag="IWO17_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291598.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2082..2972
                     /gene="pstS"
                     /locus_tag="IWO17_002759"
     CDS             2082..2972
                     /gene="pstS"
                     /locus_tag="IWO17_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ0291599.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1065 a          460 c          565 g          967 t
ORIGIN      
        1 ttacgcttta aactctttta tatttgaatt cactaagaag cataccatat ttttgaaaaa
       61 aagtgagaaa aaaggcttat aattaggatt tagagtaatt aattagatgt ttattttata
      121 ggactgatat tatagattac aaatagtatc gtatttcgca atcttaaaat tatcttctta
      181 ctgtgaataa gataataaaa tttgtatttg tcctttttag gtaacttatt ttaatctata
      241 tacctttctg gatcaacgaa agtatacttt atatagtcat aacgccgatg gtaactatgc
      301 actgtaacga taatgctgct aaaatatctt tttacgaaaa acatcacaag gaaaaactta
      361 actatggtat tatctaaaca taattttttc ggtatttaat atttaattag gaggtaatat
      421 tttgcttttt tatagtaatt ttatcttaat agtagcgata ttattacttc tgaatatatg
      481 gatttttgat aggagtagaa atgcaagtat aggttttaga actaaacgaa gtctgtcttc
      541 taaaaaaaat tgggtatact cacaaactat tttttatgga ggaattgttt taattagttt
      601 actttcttca acactttatt ctttgaatat tatagatgtt tccacctcaa attcaatatc
      661 tattattgga atcattattg ctgcaattat tacacaactg tttttagtgt ttggagagaa
      721 gaaaagaagt aaaaaataga aaaactagtg atagttttgg tgatataaga aaaacatata
      781 tgtataaaaa gaagaaagta gcttctcttt gtacatatat ggtttgtttt tatggatgcc
      841 gagctttttt ataagataag gctaccgccc ataacgaccc aaggatttgt ggaactaagt
      901 aaatacctgc aagaagcaag atcagctggg tcggttttaa aggatgatag aagtaacgcc
      961 attcagtaaa attggcgcaa aataaatagg aagtgacagt agatataata ttggcaatga
     1021 ataaagatat aatacgcctg ttcctttgaa aataatatcc agcaaaaaga gataaaaata
     1081 tcaatgtgat gaacccgctt acggaatgat aacgaaaatc aagcagtaag gcgaaatgca
     1141 cataaggaac aaaagataaa agcaacggaa ggtttttcca gcaagttttt atcatctttc
     1201 atcaccacct aacaagtgtt attattttgt tagttttatt gtaaattgaa tcctgcaata
     1261 atcaattact ttttcattat ttctagatta aatgaataaa ctctcatgcc attatctcta
     1321 aaaaaaaagt ttttccccct aattttttta gatagaaaat tgtatagtat gatgagagaa
     1381 aaagtagttt gggggaacgt taatgagaag aaaagcagta atcgatcggc tttatttatt
     1441 tttttggttt tttcctatcg ttttgctacc ttttacatgg gtaaatgctg ttgtacaagg
     1501 tgcggagata ttaggcctga tccaaggaag cggatttgtg gtttctagga attttccagc
     1561 tttgattgtt ttctatttag tgataagtgt aggtctctta ttaccaaaat ctctgatcaa
     1621 tcactacaat tggcagctgc tttacttttc ttgcttatta gtatttacat ctatgttttc
     1681 tttgctgcct cgcttgatta tcggccctaa tggtttgagc gagagtggat tgaaggattt
     1741 agcttattcc tccatgtatt tcttgacgat caaaccatgg ttctatttag taattgcctc
     1801 atttattctt gcaacatttt tttacttctg gacagaaatc aggataaaaa gtgaacgatc
     1861 tgtatctata aaaaataact attgatggtg gatcatcagt aaaaaaacat atgagctgcg
     1921 taaaatgcat gtttctcata tgcttttttt attattatgt aaagaatgcg aaaaacgttt
     1981 acatgatttt tacataactt aactatattt ttacagcaac catacgaggg atttacagtc
     2041 gactgataaa ctttagttgt aaataagaaa ggagcaataa aatgaaaaaa gtattgttta
     2101 ctctaggtat gatgggaatt cttttagccg gatgcggctc aggatcagga aatacagatg
     2161 gctctgccac acaaaataat tcaagtgact caaatgaaca agtaaaaatc gttgcggtag
     2221 gttcgacagc attacagccg ttagtcgatg cagcacaaga aagctttgta caagaaaatc
     2281 caaattatca aatctctgta caaggaggag gaagcggaac tggtttgagc caagtcgaag
     2341 ctggagcagt tactatcgga aactctgatg tatttgcaga agaaagagac ggagttgatg
     2401 cttcaaaatt agtggatcat aaagtagcag tggtcgggat ggcaccaatc gtcaataaag
     2461 atacagatgt caaagatatc accaaacagg aattgattga tatctttaca ggaaaaatca
     2521 ctaactggaa ggaagttggc gggaaagatc aaaaaatcaa cgtcgtgaac cgtgcaaacg
     2581 gcagcggtac ccgcgcgaca ttcgaaaaat ggggactgga tggtgctacc cctgtgcagt
     2641 cccaagaaca agattcatca ggaacagttc ggcagctggt aagtcaaaca ccaggagcaa
     2701 tcagctattt agctttctca tatctcgatg attccacaca agcactaagt atcgacggtg
     2761 tagaaccaaa agaagagaat gtagcagata acagctgggg aatctggtca tatgaacata
     2821 tgtacacgaa cggaaaacct tcccctgaag tccaaaaatt cttagactac atgatgacag
     2881 aagaaatcca agaaggacct gtcaaagaat tgggctattt accaatcaca atgatggaag
     2941 tagaacgtga tcacgaagga aatatcaaat aactagcaga taaacatgcc acggtagtcg
     3001 ttactattaa ggaaaaatca actatgtttc cccacatata catttttcct ttaattg
//
LOCUS       DACECR010000165         2997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.169, whole genome shotgun sequence.
ACCESSION   DACECR010000165 DACECR010000000
VERSION     DACECR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.169"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(84..1031)
                     /locus_tag="IWO17_002760"
     CDS             complement(84..1031)
                     /locus_tag="IWO17_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0291600.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1277..1741
                     /locus_tag="IWO17_002761"
     CDS             1277..1741
                     /locus_tag="IWO17_002761"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ0291601.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1803..2846)
                     /locus_tag="IWO17_002762"
     CDS             complement(1803..2846)
                     /locus_tag="IWO17_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ0291602.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          989 a          593 c          574 g          841 t
ORIGIN      
        1 tttattcgga aaaaaggact gtgacaagac ttttgtcaca gtcctttttc atttaaaaaa
       61 atgtttactt catttgacgt atattattgg tgaggcttca attcatttgg cttcactttt
      121 tctgtttctc ttatgatata aacaggacgc tgtttcgttt ctaagaagat tttcccaata
      181 tatttcccga tgattccaag acataacaat tggattccac caatgaacag gaagatcgag
      241 accatggaag gccatcctga tgtaggatca ccaaacaaca atgcccgcaa aataatgaac
      301 aaaattgcaa taccagaacc gacacaagac aaagcgccga taaatgaagc aatcgttaac
      361 ggtgcatccg agaagttcac gataccatca atcgagtagt tgaataattt ccagaaggac
      421 caagatgttt cgccagccac acgttcgcgg ttttcataag aaatatattc tgttttaaat
      481 ccgacccagc taaataatcc ttttgaaaat cggttgtatt ctgttaattc taggacggca
      541 tccaccattt gacgagtcat caagcggaaa tcacgagcgc catcgaccat ttctgtatcg
      601 ctgattttat tgatcaactg gtagaatttt ttcgcaaaga aactgcgtat cgcaggctcg
      661 ccttcacgag tcgaacgccg ggtgccgaca caatcgatat cagaagtagt gatccggttg
      721 atcatctcag gaagcagttc gggcggatct tgcagatcaa catccatcac ggtcactaaa
      781 tctcctgtag aagcttgcaa tccagcgtac agagccgctt ctttcccaaa atttctggaa
      841 aatgagacaa agcggacata aggataagct gaggctagct tcttgattgc tgctaacgta
      901 ccgtcttttg atccgtcgtc aataaacaaa tattcgaact catgggcacc tttgaatttt
      961 tcaacctcat caaaaaaaag aggaacagtt ttttcttcat taaaacatgg gacaacaatt
     1021 gatactttca tcgtttctat cctttcaatc gtatgtttat atatggttta cattcaaaat
     1081 ttaataaacc ttaaataaca cctttaaaat ttgattttac gtaaaagaac aggtattctt
     1141 tttgtaaact aacgtattta gtttaccatg tttcctcggt ttatcaaact tttaagaaca
     1201 atttttatta gttggcaagt agttttggat agagaaaaag gaggaagttc cagtctattg
     1261 actaggagca aacaaaatga aaatacacac acctatcggc tttttatggt tagatgcaga
     1321 tgaatccgga ttgactgctg tatcttttca gccaatttca gaaagcgaat cagcgaatca
     1381 gcgaatttta acgcgcacac aagaacagtt tgaagaatat tttgcgggaa aaagaaaaga
     1441 atttgatgtt cctttatcta taaagagagg tacgcctttt cagcaaaatg tctggaaagc
     1501 tttgtcgacc attccttatg gagaaatccg aacgtataaa gaagtagccg ttgctgttga
     1561 tagtccgaaa gctgtgcgag cgattgggca ggcaaatcga accaatccat tgccaatcgt
     1621 tattccttgt catcgagtga tcgggcaaaa tggaaaactg acaggatata tgggaaacgc
     1681 aatagatggg gttgaaatca aacgccaatt gttgttgtta gaaggatatc tgcaaaatta
     1741 aaaacagttg tgattcggac attgttccat gcgagaatca caactgtttt ttcatgttca
     1801 ttctaaactt tgatcagcca aataaatagt aaagccatca ctgtttggag aagcccgaca
     1861 gctaatccat aagataagaa gcgtgtacct tctttcatga attttgcaaa gtctaaacgc
     1921 agaccaattg ccgctaaagc agtgatctca aaccagctgc tgataaaatg tgccgattgg
     1981 tcaataacta caggtaaaga aaacaagctg ttgaccaaac aaaagatcac aaaccctaat
     2041 acataccaag gaagcggtaa ttttcgtttt ttctgcgtat cagttggcgt ttcaaacaga
     2101 gctggttgtt tcttgtgcat gaatttttca aacagataga caacaacaac gagcatcata
     2161 atacgtagaa tcttaaatag catggcgaat tcgacaacag aattgtcaag taagcttgcc
     2221 ccagcgacta cttgaccaac agattggagt gtaccaccca aaagtgcact tttttcaatg
     2281 agatttccac caaacaactg taatccaaga aaagggagag tcaacatcat cacagtaccc
     2341 agtagattga ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg gatggaaggg
     2401 gcgattgaag caatagcaga agaaccacaa acagcgtttc ctccagccat catcaaagaa
     2461 gaagtatcgc tgaattgcag ttttttacca atcaagtaag caccgatgat agtgactgac
     2521 atttgcagta acacaaaaac aattcctttt acaccaattt gagcaatcgt ttgagctgtg
     2581 acagttgcac ctagtaaaaa aacagagaat tctaatagtt tgctttcagc aaattttgtg
     2641 ccgattgcta gaacaggctg tttaaaaaaa gtatttccta ataaaatacc caataaaata
     2701 gcaatagtcg ctccacctaa ctgtggcaga taaatagcaa gaagcctgct gaaggcagcg
     2761 atggcaaaag aaagcagtaa accgggtaag atttcgcgga ccgctgaatc ctttacagat
     2821 tttattggat gtgacaatga tttcatttga tcggttcctt tcgtcaataa atactaaaag
     2881 catactcgaa catagtagca ctaaaccagt attttgacaa aagaacaaga ggtcgtgaca
     2941 aaagtcttgt acgacctctt gtttcgaata aacggtgttc cagaagtagc ttctacg
//
LOCUS       DACECR010000166         2939 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.170, whole genome shotgun sequence.
ACCESSION   DACECR010000166 DACECR010000000
VERSION     DACECR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.170"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(21..956)
                     /gene="murB"
                     /locus_tag="IWO17_002763"
     CDS             complement(21..956)
                     /gene="murB"
                     /locus_tag="IWO17_002763"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ0291603.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1029..1949)
                     /locus_tag="IWO17_002764"
     CDS             complement(1029..1949)
                     /locus_tag="IWO17_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0291604.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2062..2814
                     /gene="xth"
                     /locus_tag="IWO17_002765"
     CDS             2062..2814
                     /gene="xth"
                     /locus_tag="IWO17_002765"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ0291605.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          824 a          594 c          509 g         1012 t
ORIGIN      
        1 attttaagtt ccattactta ttaattggct aaacttctgc ttgtttctga tattgcttgt
       61 ttttctccaa tgatccgcac ttctgtttct aaagacacat caaatttttc tttgattact
      121 tcttggatgt gcgcaatcaa ttcaatataa tctgtcgctg tcgcatgatc gatattgacg
      181 atgaatccag catgtttttc agagacttgt gcaccgcccc atttcaggcc ttgcaatcct
      241 gcttgttgga tcagctgacc tgtatagtgt cctactgggc gcttaaagac acttccgcat
      301 gaagggtatt ctaatggctg ttttgattga cgcaattcgg tcaactcatc cattcttgct
      361 ttgattgctt cataatcccc tgtttgcaaa ctgaatcgag cttccaatac gatagcacgt
      421 aattcttgga ctttactgtg acgataagaa aataccattt cgtcttttgt caaagttttc
      481 aatgttccat cttcaagcaa aagatccact tcagcaaaaa catctttgat ctctccgcca
      541 tacgcacctg cattcatata aaccgctcca ccgacacttc ccgggatgcc gcaagcaaat
      601 tcaaatccag tcaatgattc tgccaaagct gcataagtcg tatcaatcag tgaggcacct
      661 gcttccgcaa tgaccatcgt tcctttgaca tgaatctgtt tcatttcagt cagcatgatg
      721 actacaccgc gaatcccgcc atcttgaacg atcagattgc ttgcatttcc taataccatc
      781 caaggtatat catggattcg gcaataatcg atgatttgct ttacttcttc ttgttttttc
      841 gggaatgcaa ggacatcagc tggtccacct gttttcgtaa acgtatagtt catcaatggt
      901 tcatcaaaca atatagtaat cgtggctaag ctttcaacta tttcttcttt attcactgag
      961 gattttcctt tcttttctca aacgtcctca ttattctatc acgcttaata caatcttgcc
     1021 agtcttgttc aaagttgatg acggtagtga tctttaagca cttcctcaca taattcatga
     1081 cttcttacaa cttctttttg tttaagcaat tctgtttccg atttacctaa acggatctga
     1141 gaaaagaagg cctctaccat tggctcaaac ccgcgtttga ctagcgtgtt tgtccagtcg
     1201 ccaaattctt tcactttttt tccatcagca tcttgaatga caagctgggt cagatcgttt
     1261 acttcataag taccattttt acttgttact tgatattttt ctatattagc gccacttgct
     1321 aaatccatcg ttaaaatagc cgtttgattt tgtgtttcta attgtaaaat ggcatattcc
     1381 aaaaattcct ttgtttctct tatctggtat tttgttctca aaatgggttc atctaataaa
     1441 tacacagctg tatcaataag atgtaaaaac aaatcataaa ggacgaactc tgtcgtttct
     1501 tttgccgcta ttcgattttt ttgcaggtgg atcaaccgct tatctgggat acgtttcaat
     1561 tcttctacca taggagcaaa gcgccggttg aaaccgatca tcaaaattat ttggttctta
     1621 tcagctaatt cttgcaattc acgaacttca tttagatgga cactcagtgg tttgtcaata
     1681 taaacatgga taccattttc tagacatttt ttggctaatt cataatgtac ttttgttgct
     1741 gcgtggatca tacatgcttc gattttttct tctaaaagct catcgatcgt ttcatataga
     1801 tgttggaacc cgtactcttt ttgaattctt tcttttgttt ctttatttct tgttgcaaaa
     1861 taaaaatcaa gtgttccttg ttttttcgca taagttggta aatatgcttt ttctgcgata
     1921 ttccctacac cgattacacc aatcttcatc tactttcctc ctggatcatt tagttacttt
     1981 catcttacac cattttttac taaaatgagg tacaatagta tcgcaaaggt aaggaaatta
     2041 attcgaagga gtgtgctttt attgaaattt atctcttgga acgtaaacgg gcttcgtgcc
     2101 atcgtaaata aaaatttttt agaagttttt catgagttag acgccgattt tttctgtctg
     2161 caggaaacga aactccaagc aggacaaatt gatttagatc ttccaggtta ttatcaatat
     2221 tggaattacg cggaacgaaa aggctattcc ggcacagcga tttttgctaa aaaaccagca
     2281 ttgaatgcca cttacggaat gggtatcgat atccatgata cagaaggccg tctgattact
     2341 ttggaatatt ccgatttctt tcttgtaacc tgttacacgc caaattctca aagcgaatta
     2401 aaacgtttag attaccgatt agaatgggaa gaagcctttt ataattattt ggaaaatcta
     2461 aaaaaacaaa aaccagtcat cgtgtgtggg gatctaaatg tcgctcatca aaagattgat
     2521 ttgaaaaact ggaaaacgaa tcaaaaaaat gctggattca ctccagaaga aagagcggct
     2581 ctttctcgac tgttggataa cgggtttatc gatacattcc gttattttta tccaacccag
     2641 gaaggtgtct actcttggtg gaactaccga ttcaattcaa ggaaaaacaa tgctggttgg
     2701 cggatcgatt actttttgac gagcaaagat ttagagccag gactagctga tgcaaagatc
     2761 catacggaca ttatgggcag tgatcattgt ccagtagaat tagatttgaa ataaagaggt
     2821 tgtgaaaaag tcgtttagtt tcgagtatta agaaaaattt tgtgaaaatg gctttccaca
     2881 ttttgcataa atttggcata ataccgagaa actggctttt gaacactgtt tatcgagag
//
LOCUS       DACECR010000167         2912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.171, whole genome shotgun sequence.
ACCESSION   DACECR010000167 DACECR010000000
VERSION     DACECR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.171"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(7..>2912)
                     /locus_tag="IWO17_002766"
     rRNA            complement(7..>2912)
                     /locus_tag="IWO17_002766"
                     /product="23S ribosomal RNA"
BASE COUNT          612 a          879 c          600 g          821 t
ORIGIN      
        1 taacctattg gttaagtcct cgatcgatta gtatcagtcc gctccataca tcactgtact
       61 tccactcctg acctatctac ctgatcatct ctcagggatc ttactttctt aaagaaatgg
      121 gaaatctcat cttgaggtgg gcttcacact tagatgcttt cagcgtttat cccttcccta
      181 catagctacc cagcaatgcc cttggcagaa caactggtac accagcggta agtccatccc
      241 ggtcctctcg tactaaggac agctcctctc aaatttccaa cgcccgcgac ggatagggac
      301 cgaactgtct cacgacgttc tgaacccagc tcgcgtgccg ctttaatggg cgaacagccc
      361 aacccttggg accgactaca gccccaggat gcgacgagcc gacatcgagg tgccaaacct
      421 ccccgtcgat gtggactctt gggggagata agcctgttat ccccagggta gcttttatcc
      481 gttgagcgat ggcccttcca tgcggaacca ccggatcact aagcccgact ttcgtccctg
      541 ctcgacttgt tggtctcgca gtcaagctcc cttctgcctt tacactcttc gaatgatttc
      601 caaccattct gagggaacct ttgggcgcct ccgttacctt ttaggaggcg accgccccag
      661 tcaaactgcc catctgacac tgtctcccac cacgattagt ggtgcgggtt agagtggcca
      721 taacgcaggg gtagtatccc accagcgcct ccatcgaaac tagcgttccg atttctacgg
      781 ctcctaccta tcctgtacat gcggtacaga cactcaatat caaactacag taaagctcca
      841 tggggtcttt ccgtcctgtc gcgggtaacc tgcatcttca caggtactaa aatttcaccg
      901 agtctctcgt tgagacagtg cccaaatcgt tacgcctttc gtgcgggtcg gaacttaccc
      961 gacaaggaat ttcgctacct taggaccgtt atagttacgg ccgccgttta ctggggcttc
     1021 aattcgtacc ttcgcttgcg ctaagcactc ctcttaacct tccagcaccg ggcaggcgtc
     1081 agcccctata cttcatctta cgattttgca gagacctgtg tttttgataa acagtcgctt
     1141 gggcctattc actgcggctg atcgtatgat cagcacccct tctcccgaag ttacggggtc
     1201 attttgccga gttccttaac gagagttctc tcgctcacct taggattctc tcctcgacta
     1261 cctgtgtcgg tttgcggtac gggccgctgt tttctcacta gaagcttttc tcggcagtgt
     1321 gacatcagga acttcggtac tattatttcc ctccccatca cagcttgtcc ttaagaaaag
     1381 aagcatttga ctcctcttca gacttactgc ttggacagac atttccgatc gtctgcattc
     1441 cttagcctcc tgcgtccctc cattgctcaa acaaaaacaa cgggtacagg aatatcaacc
     1501 tgttatccat cgcctacgcc tgtcggcctc ggcttaggtc ccgactaacc ctgggcggac
     1561 gagccttccc caggaaacct tagtcattcg gtggacagga ttctcacctg tctttcgcta
     1621 ctcataccgg cattctcact tctaagcgct ccagcagtcc tcacgatctg ccttcaacgc
     1681 ccttagaacg ctctcctacc aatacaccta aaggtgtact ccacagcttc ggtaatatgt
     1741 ttagccccgg tacattttcg gcgcagggtc actcgactag tgagctatta cgcactcttt
     1801 aaatggtggc tgcttctaag ccaacatcct agttgtctgt gcaaccccac atccttttcc
     1861 acttaacata tattttggga ccttagctgg tggtctgggc tgtttccctt tcgactatgg
     1921 atcttatcac tcacagtctg actcccggat atgaatgaat ggcattcgga gtttatctga
     1981 attcggtaac ccgagatggg cccctagtcc aaacagtgct ctacctccat cattctcaat
     2041 tccgaggcta gccctaaagc tatttcggag agaaccagct atctccaagt tcgtttggaa
     2101 tttctccgct acccacacct catccccgca cttttcaacg tacgtgggtt cggtcctcca
     2161 gtgcgtttta ccgcaccttc aacctggaca tgggtagatc acatggtttc gggtctacga
     2221 ctacatactc aaacgcccta ttcagactcg ctttcgctgc ggctccgtct cttcaactta
     2281 acctcgcatg caatcgtaac tcgccggttc attctacaaa aggcacgcca tcacccatta
     2341 acgggctttg acttgttgta ggcacacggt ttcaggttct atttcactcc ccttccgggg
     2401 tgcttttcac ctttccctca cggtactggt tcactatcgg tcactaggga gtatttagcc
     2461 ttgggagatg gtcctcccgg attccgacgg aatttctcgt gttccgccgt actcaggatc
     2521 ctcctaggtg tcttccacat ttcgtctacg gggtttttac cctctttgac tgacttttcc
     2581 aagtcattcg actatctgaa agaactacca tattggagtc ctacaacccc aacaagcaag
     2641 cttgctggtt tgggcttttc ccgtttcgct cgccgctact cagggaatcg aattttcttt
     2701 ctcttcctgc aggtacttag atgtttcagt tctctgcgtc tacctcgaat gtgctatgta
     2761 ttcacacaat cgtaacatcc tattaaagat gttgggttcc cccattcgga aatctctgga
     2821 tcatagctta cgtacagctc cccaaagcat atcggtgtta gtcccgtcct tcatcggctc
     2881 ctagtgccaa ggcatccacc gtgcgccctt at
//
LOCUS       DACECR010000168         2823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.172, whole genome shotgun sequence.
ACCESSION   DACECR010000168 DACECR010000000
VERSION     DACECR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.172"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            949..1422
                     /locus_tag="IWO17_002767"
     CDS             949..1422
                     /locus_tag="IWO17_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ0291606.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="IWO17_002768"
     CDS             1431..1658
                     /locus_tag="IWO17_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0291607.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="IWO17_002769"
     CDS             2294..2665
                     /locus_tag="IWO17_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291608.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT          904 a          456 c          417 g         1046 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atg
//
LOCUS       DACECR010000169         2816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.173, whole genome shotgun sequence.
ACCESSION   DACECR010000169 DACECR010000000
VERSION     DACECR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.173"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            188..511
                     /locus_tag="IWO17_002770"
     CDS             188..511
                     /locus_tag="IWO17_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291609.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            675..929
                     /locus_tag="IWO17_002771"
     CDS             675..929
                     /locus_tag="IWO17_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291610.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            999..1244
                     /locus_tag="IWO17_002772"
     CDS             999..1244
                     /locus_tag="IWO17_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0291611.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1320..1685
                     /locus_tag="IWO17_002773"
     CDS             1320..1685
                     /locus_tag="IWO17_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291612.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1746..2309)
                     /locus_tag="IWO17_002774"
     CDS             complement(1746..2309)
                     /locus_tag="IWO17_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291613.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          834 a          493 c          432 g         1057 t
ORIGIN      
        1 catcataaat aaaatttatt tttgttactt ttcaactata gcacgaatga caaactctca
       61 caagtttcaa aatataagta aaataatatt gttattctta tttcttttac ttgtgtcata
      121 actattttgt tactttaatt tttatttgct tattttagaa aaaatgttat tcttttattt
      181 attctcaatg ttaggagtga ttattttgcc atttattgcg attttattcg atttagttgc
      241 ggcagcagct tattttctcc aattaaatta tcaaacctca ggaattctgt ttattggaat
      301 gatttttcaa ggtgtgatta cccttttgct tcttgtcatg atgctcagct ataaaggaaa
      361 aagatatgcc cgtattcaaa caaaagtatt tgttaaatat gtttccattc gctatgggat
      421 catcattctt tcctttttag tgaatgcgat cgtcctcttt ttatatgtat tgaattactt
      481 agatatcaat ccattgattt tttctcgata gataagacac attcagcgga acagttcctg
      541 cttatcagca gaaaactgtt ctgctttttc atttttcatt gatctattct tcttttattt
      601 tcctagcatt tcatttgtga tgtatgcaat aacatgttat cttttattat atgaatcgtt
      661 gaggagtccg ttacatgatt acgttggtga gagttttgtt ttgggtgcct gcggtcgcac
      721 tggtcgcaag tattgtttat cttatgaatt ggaacaaaga acggttttat ttagctattt
      781 tgactcttcc ggctatctac tttatgtgga aagtttttaa ttacaactac tttgaacccg
      841 atagtgtttt tatagaagaa ttatcaggac tggttttgtc tttattgatc gtcattcttt
      901 atttgatacg tttaaataaa aaacattgag tactattgat tgcatgtgta gctttctata
      961 gtcattataa ctattctact ataatgaaag gtgattaaat gagtatcgaa gaagctattt
     1021 tactggatac tttacgttgt cgtctgaatt tcttaagaac tggccgatct ttatctcaag
     1081 tagctgtttc tagcgcgtta agtattcctt tattcaaata tcgtaagtac gaagaaggag
     1141 aagaaattcc aagcatcatt gaattaatct atatcgctga cttttacgat atttccgttg
     1201 attacctaat tggtcgctca gaaacagcaa gcaaactgaa gtgatttttt ccagccttgg
     1261 tgctggtttt ttcatttaac gtctaaggtt aacaatctat atataaagga gacatttcta
     1321 tgcgattatg gcatgaaaaa ttgatttcaa agcttcctcg tcaacagctt ttaggacaac
     1381 atcgggaatg ctgtgcatta agaggaaatg gctggggaaa gaaacatgct actgtggatt
     1441 atgtttttca ctattctcct tatagattat tccaatatca tcagcttgtc atggatgaaa
     1501 tggaaaaacg tggttacagc ccagcaccag agtggaaaaa tcctatgtat cgtggcaaat
     1561 catgcgaagc attttttgat ttacctactg tagagcgcaa cgtgccgatt tatccggaac
     1621 acgatgaagt ttatctagca gaatgtttag caaatttgga gcaaaaagga atttatctta
     1681 cctaatataa ctagaaaagc cttctatctc ctcttacttc attaaaaagc tttaaccatc
     1741 ttagatcaag ctaaaatgtc gtaagtttta gggttatctt catcctcacg tgcaaaacta
     1801 aaagcgtact ctagttttgg ttcatcgcca acttccctag acataaatga acatctcctg
     1861 acgtggttta aaagtaccgc atcttcgacg tctgtatacg atgcacagca cagattgctt
     1921 cttttgatct ttcttttgat ttttctgttg cttcttctac accttcttcg attactacat
     1981 cgatgcttac aattgcttct gcctaatatt cccatgcatc gtcgatttaa gcctctgtag
     2041 tcactgtgat cgtttccaca ttttaactcc tctgtcgtgg cttcagttga ttttgtgatc
     2101 agttcagttg ctaagccaga gtcagaacaa cctgccattg ctagcgttaa tattgatagt
     2161 aaaaatagtt tttttatcat aaggttcccc ccctgaaaat agactaaaat aataacgctt
     2221 tctttttttg ttttcagacg aaaattaata atttctaaat attctttaaa aaagcttata
     2281 actattatgc cttttttaat atataacaca gttattttta aaaataagaa tgctaaatca
     2341 ctctacaata acggcgaaag cacaaaaaat aaaaaaatta ttacgttatt aaaaaaacag
     2401 atgaaagatc aggttttatt agatctcatt ctttcatcac ttgaagggat ttaatgaact
     2461 actttttcct ctttgtcgtt tttatatatt cgtattcctt caaacagtag gatagcctct
     2521 ttcaatgctt gatatttgaa ctgtccgtta cttaaaaatt tagatgtgcc gccctcttcc
     2581 tagaacatat tcttttttac tttttcatta attgacagac caatcgaacg aaactgtaaa
     2641 tttcgatagg ataatgattg gtcaaactca ttcattttca agacatctcg attcatattc
     2701 aagataggaa tctttttatc aacatcactt tattcgtatt ttcctctacc ataaaagctt
     2761 cacgctccac aaaattgttt tatacactac tattcagccc attcaacgac caatga
//
LOCUS       DACECR010000170         2784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.174, whole genome shotgun sequence.
ACCESSION   DACECR010000170 DACECR010000000
VERSION     DACECR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.174"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..135
                     /locus_tag="IWO17_002775"
     CDS             <1..135
                     /locus_tag="IWO17_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0291614.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="IWO17_002776"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="IWO17_002776"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ0291615.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="IWO17_002777"
     CDS             complement(2412..2663)
                     /locus_tag="IWO17_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ0291616.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
BASE COUNT          746 a          618 c          494 g          926 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatt
//
LOCUS       DACECR010000171         2740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.175, whole genome shotgun sequence.
ACCESSION   DACECR010000171 DACECR010000000
VERSION     DACECR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.175"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..221)
                     /locus_tag="IWO17_002778"
     CDS             complement(<1..221)
                     /locus_tag="IWO17_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0291617.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLR"
     gene            complement(235..1452)
                     /locus_tag="IWO17_002779"
     CDS             complement(235..1452)
                     /locus_tag="IWO17_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0291618.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(1442..>2740)
                     /locus_tag="IWO17_002780"
     CDS             complement(1442..>2740)
                     /locus_tag="IWO17_002780"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0291619.1"
                     /translation="VLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKL
                     GTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHM
                     VSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYN
                     LAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDD
                     SRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLED
                     GSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENI
                     QKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKK
                     IRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT          750 a          586 c          455 g          949 t
ORIGIN      
        1 cgtagcaata attcggtttt gatcgtttca aactctttat cggtcaaagg agaatcattc
       61 agttctgctt gattatttga gatgattttt tgacgtaaat tgctccaaag atcttcttct
      121 gattttagat ccggacgata cgtccactga ttatgtcctt ccccgaggac acgaatcaac
      181 tgttcttcga cttccgcttc ctcacggtga ctaattttca ttcttcttcc ctccctaaac
      241 aaacatcttt tgcaagaagc cttttttcgt ttccttcagc aaatctaact tacgttgatg
      301 aagagcgatc gtatcatcta gctgcttgaa gaaggcacca attttttgtt gttcatttat
      361 atccggtgga aactgaaatg tcctttgttc tataccacct ttagtcacat gtaccattgt
      421 cgttccgtta gttgattctt taattttctc tttatcagtc aatagccaag tatatagata
      481 ctgtttgtcc aattccttag ataaaaattt aagtttccaa atatgataat ggtagattac
      541 acgttcttca ttccacattt caggaccgaa gcttgttgcc cataaatata agagatctcc
      601 tttattagca tacttgtctt cctctaattc taagtttgaa taataccatc tatcattagt
      661 attaaaatta cctactctta aaaccctata ttttccagaa tcaagtaatt cttgttgttt
      721 aaaagctcta ccattcaaaa aaactaccac ttcccccaac ttccgctctt cccaatctcc
      781 cgtaaatcct ggaaaacgaa cttctggcac ttttgctccg tttttaggga acattttttg
      841 taagaatcct tttttcgttt ccttcagcaa atctaattcg tgctggtgaa gcatgatggt
      901 gtgatcaagt tgtttgaaaa atgaaccgat cttgatctgt tcgtcataag ttggaagtga
      961 gataggtgtc tttttaccac tatcaattgt ataggttccg acggttccaa tgcctgcaac
     1021 gtcacggata tacttctgaa ttttgttatt tatgaacgag tagtaaaaga agtttccgtc
     1081 aattttatgc tcattcttga accaaatgat gtttccatcc ttaaagtaaa ttgggttatc
     1141 atcagtgatt aataagggga cacctattga tccgacacca gttacaagca agtctccttg
     1201 agaaacttta cctgaatttt tcgaatattc attatatttt tcttctgaaa tgtaaagata
     1261 atcggatggt tcctcatttt ttgcagctga gacaatatca cgagcacgta aaaatctaat
     1321 acctgagtta gtccaatctg attgatgaat tcgcttcaca gaagtaacat ccatcatatc
     1381 tcctaactta cgctcttccc aatcttccgt aaaaccagga aaccggattt ctggtcgtgt
     1441 atcattgctc atgtttgaac acctccagcg caccttgtaa ccacgcttca ttttcaggtg
     1501 ttgtttgcag ggaagagata gcttctaaaa gttctttttc tagcacttgc tgttcttgtc
     1561 gtatcttctt gatgtcattt ccgacatgaa ccatatcaat tggttcttct tcttcgaaag
     1621 tatcgacata gcgtgggata tttaagttat agtcattttc tttgatttca tcaaaggttg
     1681 ccaaatgagc gtatttctcg acatcttttc tttcagcgta ggtttcgagg attttttgaa
     1741 tgttttcttc cgataattta ttctgatttt tccctttaac aaattcacga ctagcatcaa
     1801 tgaataatac atctcgagtt tgacgattct tcttcaatac aatcaccgtc gttggaatcg
     1861 atgtaccaaa gaacaagttc gcaggcatac cgatcacagc ataaatactg ccgtcttcta
     1921 ataatttttg acgaatgacc ccttctgcag caccacggaa taaaaccccg tgtggcaaga
     1981 cgatagccat agtccctgtt tccttcaaat gatagaaacc atgaagaaga aacgcaaaat
     2041 ctgctttgga ttttggcgct aatttcccgt agcgattaaa ccgagaatca tctaagaagg
     2101 tcgtatctgc tgaccaatta gccgaataag gtggattcat cactactgca tcaaatgtgt
     2161 aaggttcatc tgttggccag tccttattca acgtatcccc attgcggaga ttcatttctt
     2221 cggcatctac cccatgtaaa atcaagttca tttttgctag attgtatgtc gttgtattta
     2281 gttcttgccc atgatattta acgttatctg gatgagttaa gtagttacgt acatttaaca
     2341 tcaaggaacc tgaacccatg gttgggtcaa agacactaaa gaatcgtcgt tctttttgat
     2401 ccaacgtcac gatttgtgcc atcatatcag aaaccatatg aggcgtgtaa aattctccgg
     2461 ctttcttccc tgcttctgaa gcaaattgac tgatcagaaa ttcatacgca tccccgatca
     2521 catccccatc gtgacctaaa acttccacgt cattcaattt cttgataact tcggtaatcg
     2581 tcacatttcg ttgttgctca tctgttccta gtttttttga ttggagatcc acatcatcaa
     2641 ataacccatt aaattgattg taggtagatg ccaattgcac aaaggctttg tttagatcgt
     2701 tcaattggaa cgttgcttgt tttgcttgat ccgctaagac
//
LOCUS       DACECR010000172         2714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.176, whole genome shotgun sequence.
ACCESSION   DACECR010000172 DACECR010000000
VERSION     DACECR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.176"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            385..1518
                     /locus_tag="IWO17_002781"
     CDS             385..1518
                     /locus_tag="IWO17_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ0291620.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            1549..1869
                     /locus_tag="IWO17_002782"
     CDS             1549..1869
                     /locus_tag="IWO17_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291621.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(1898..2362)
                     /locus_tag="IWO17_002783"
     CDS             complement(1898..2362)
                     /locus_tag="IWO17_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0291622.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(2494..>2714)
                     /locus_tag="IWO17_002784"
     CDS             complement(2494..>2714)
                     /locus_tag="IWO17_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0291623.1"
                     /translation="SVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAV
                     NLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT          879 a          533 c          499 g          803 t
ORIGIN      
        1 cgtttagttt cgagtatgaa gaaaaatttt gtgaaaatgg ttcctcacgt ttttgcataa
       61 atttgactta ataccgagaa actgatccct tctgtcataa cctcttttta tttatgtctg
      121 tttctttttg aaaacaacta ttttttcaac gtttactctc ttgccaatcc aacatcatct
      181 aacggataca actatttcaa tgattaccat tacactaaaa aatcggaaac acacctctcc
      241 gaacacaaat acataagact atagaatgtt tcatttaaga aaaaaagcgt ttcattatca
      301 aaaaggtaag atctccttta aaatgaaatt atctgtagta ctaataatgc agacaatttt
      361 atgaatctga aaggagcatc tccaatgaaa gcagtcactt ggcaaggaaa acagaaaatg
      421 gaaatcagaa acgtagatga tccaaaaata ttagaaccaa cagatgcaat catccgtatc
      481 acagcaacag ccatttgcgg ctccgatctg catctttacc atcatggtga agcagttttg
      541 gaaaaagggt atgttgtagg acacgaacca atgggaatcg ttgaagaagt gggatctcaa
      601 gttcaaacat tgaaaaaagg gcaacgtgtg gttatccctt ttaatattag ttgtggtcat
      661 tgtcatttct gcctgaatca catggaaagc caatgtgatg aatcaaatcc agatcagtta
      721 tatggcgggc ttttcggatt tggcaagttg aacggtaatc attggggtgg tcaagcggaa
      781 tatctaagag ttccttatgc agactctact tcatttgttg taccagataa tgatcttcct
      841 gatgaaaaag ttttatttct atcagatatt ttgcccacag cttattggag cgttgaaaac
      901 gcaggagtaa aaaaaggcga tacagttgtg attctaggtt ctggtccagt cggtctattc
      961 gctcaaaaat tcgctgttat ggctggagca gagcgcgtaa tcgcagtaga tccagtacag
     1021 caccgtctag ataaagcggc taattacaat ggggttgaaa cttatttctt agaagacacg
     1081 gcacaagctg gaacagaatt gtatgaactg gctaaaggtg gcgcagatgt cattatcgat
     1141 tgtgtaggaa tggacggatt agagcctgtc aaagaaaaag cgaaaaattt agtaagctta
     1201 caaagcggca caatctctcc attacagatg gcatcgcaag ctgtcaaaaa atttggaaca
     1261 gtccaagtaa ctggtgttta tatgactcca gcttcttctt acccgctgca agaatttttt
     1321 atgcgggata tcgctgtcaa gcatggacag gcaccaatta tccatctaat gccaaaaatt
     1381 tacgcaatga ttgcagaagg actattcgat cctacacaaa tcattactca ttcgctgcca
     1441 ctatcagaag cagcacaagc atatgagatc tttgacaaaa aagaagataa aaatataaaa
     1501 gttgttttga aaccttgaat tcagtaaaaa atacaggtga ttttctttat gcccaaaatc
     1561 aaacgaaata cccatgagat cgaaatagga aaagaagaag atatcgaaat acgaggacgt
     1621 cgcttccgct tctatttcca aaatcgttac acattgatct cactggctgt cgatttatta
     1681 accgggattt tctatatttt tggaagcctt gcttccttaa ctaaaatccc cgatcgatat
     1741 ggcacctatt tttatttggc tggtgctatt ttcttgacga ttcgtcctat tctcagaatc
     1801 gtaaggaaca tttttattta cgacgaaaaa aaagcaatga agagaacaag caaaaaaaaa
     1861 gaggcatgag aatttttcaa atctcatgcc tcttttttca ccctatcgta tcactttgat
     1921 acagacgagc ggtcgtccca ctttcttcct tgaccattgt caaaaatgtc cactcgtatc
     1981 gttcataaaa cgtggtatga tctgtaatta agtaaagttt ttcgtaccca agttgatgca
     2041 tttcttttct gatttccgct aatatccgct tggctatccc ctgcttacgc ttttccggtt
     2101 caacaaacag ggcacaaaga tttggtgtca gatcagggcg gttatggaag tcgttttcaa
     2161 tgacgcccgc tcctccgacg atggtttctt tttcatctaa gaccacatac cattgaggaa
     2221 tactttcctt attagcgatt ccagtacgaa tactttcctc atacacaaca gcaggaactc
     2281 cccattttga tgaaaaccaa ttcgcgcttt gttttactaa ttctgggtgt tctcttaatt
     2341 tgatccagtt atatgtaacc aatgtttaaa cctccgacta aaataaaacg tttacaaaaa
     2401 ttatacaagt ctttatgaaa attagcaatg acaacaatag tgatgtaaaa aaaaaaacgg
     2461 aaaacaatca cttgttctcc gttttttgat taatcaagca tctaaagctt cttttcttct
     2521 ttcttggatc gtagctacct tgacgaacgg tagatagatc aaaatagaaa gaaccaaatt
     2581 gacagcggct agtacaccac ctgcaatact ttttgttgcc aatacaccgc caataattgg
     2641 tggagttacc catggaggca taacggtagc tgctggcact aaacctgtag aagtcgcaaa
     2701 gtaagcaaca ctaa
//
LOCUS       DACECR010000173         2613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.177, whole genome shotgun sequence.
ACCESSION   DACECR010000173 DACECR010000000
VERSION     DACECR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.177"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            316..570
                     /locus_tag="IWO17_002785"
     CDS             316..570
                     /locus_tag="IWO17_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0291624.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="IWO17_002786"
     CDS             571..888
                     /locus_tag="IWO17_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0291625.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="IWO17_002787"
     CDS             1447..2382
                     /locus_tag="IWO17_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291626.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
     gene            2476..>2613
                     /locus_tag="IWO17_002788"
     CDS             2476..>2613
                     /locus_tag="IWO17_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0291627.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          958 a          375 c          483 g          797 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagagaaaaa
     2401 tgtctaaaca tgttggtaaa tcaacaaaaa aacgcataga gtagtctcaa aactcccaag
     2461 aaatgaggac tacatatgcg ttcatcaaaa tatactgaac attatacaga tacttttata
     2521 acattcaagg aaattatgag aactgtttct aacatatacc acaattgtgt accagataag
     2581 attaaaaacc gaagaaatac tgatcaactg aag
//
LOCUS       DACECR010000174         2527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.179, whole genome shotgun sequence.
ACCESSION   DACECR010000174 DACECR010000000
VERSION     DACECR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.179"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..549
                     /locus_tag="IWO17_002789"
     CDS             <1..549
                     /locus_tag="IWO17_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291628.1"
                     /translation="IEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR
                     FEGVTGRKISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKC
                     ITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFK
                     NKYQIKGIKLNQETIKKLRIGG"
     gene            556..1407
                     /locus_tag="IWO17_002790"
     CDS             556..1407
                     /locus_tag="IWO17_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0291629.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            1419..1688
                     /locus_tag="IWO17_002791"
     CDS             1419..1688
                     /locus_tag="IWO17_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291630.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1850..2152
                     /locus_tag="IWO17_002792"
     CDS             1850..2152
                     /locus_tag="IWO17_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291631.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            2149..2310
                     /locus_tag="IWO17_002793"
     CDS             2149..2310
                     /locus_tag="IWO17_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0291632.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEG"
     gene            2313..>2527
                     /locus_tag="IWO17_002794"
     CDS             2313..>2527
                     /locus_tag="IWO17_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291633.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGL"
BASE COUNT          965 a          411 c          560 g          591 t
ORIGIN      
        1 attgaaattc gttttattga tcctagacgt ttcacagtac agcaacgaaa cttcatctat
       61 gcactcatag gcgatatttt catcgataca ggcatgccaa cggacttctg gaaggaattc
      121 ttttacttcc gctttgaagg tgtcacaggg cgcaaaataa gcctcaaaga tgaatcgaat
      181 acgactgtga gtgatgctaa cgtcttagca aatatcatct tagatttcat ctttgaacat
      241 catattcctt tcaaagaagg ctatgagatt ttaccagcga atcaagaata ttacttctac
      301 aaatgcatca caaaaagagt ctgttgcatc tgtggcaaaa caggagctga tatcgatcac
      361 tttgacaaag cgctaggaag acgaaagcgc aaagaagttg atcattcaga gtacacattt
      421 gcagcactct gcagaatcca tcacacggag aaacacaaga taggtgtgat caatttcaaa
      481 aataaatatc aaatcaaagg gatcaagtta aaccaggaaa caattaagaa acttagaata
      541 ggaggataaa aaatattggc tgacaacaaa cgatactact atttaaaact aaaagagaac
      601 tttttcgata gcgacgagat ggttctttta gaaagtatgc cagatggata catttattct
      661 aatattcttc tcaaacttta tttaagaagt ctgaaacatg aaggcaaact aatgtttaat
      721 gacaggattc cattcaattc tacaatgctt gcgactatta caagacactc tgtgggagtt
      781 gtagaaaaag cagtacaaat atttcgtgat ttacaactta ttgaggtatt agataacgga
      841 gcaatttata tgtctgatat acaaagtttt attgggaaat cttcaactga agctgatagg
      901 aagagaaaat acagaaaaga aattgaggaa gcaaaacaga atttaataac aagtggacaa
      961 atgtcggaca aatgtccgga caaaactaca ccagagttag agatagagtt agagaaagat
     1021 atagagttag agaaagatat agagaatgta acgccttcga aaaaatcgaa ggctaagccc
     1081 gtccgtcata aatacggaga gtataaaaat gttcttttgt cagatgagca gatggagaaa
     1141 ctcaaaatag aattccctaa tgactaccaa gagcgaatag aacggctatc tgagtattgt
     1201 gaatcatctg gtaagactta taaaaactat ttggcaacta ttcgaagctg ggcaagaaaa
     1261 gaaaaaagtg aatctaagaa cgcaagcagt ggatacaagc gcacaggaag acgagagaag
     1321 cttcctgagt gggcaatcga ccaagaagcc tatctcaaga aaaaagcgct agaacgagct
     1381 aatagacaat caaaagcacc attctaagag gtgggaaatt gaaaatcgat tatctagaac
     1441 taattaatga aatagcaaag tataaaactg gtgaggaaat agaaatcctg agagacgtat
     1501 atgatcaact cgaagaagct ggaatcgaag gaattaagaa tgatcgttcg agttggagta
     1561 agctcagata ctatttcgca ctttatatcg atacaacaca attaagaaat ttagcatata
     1621 ccaaattact atttgttgat tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc
     1681 aggtgtaatc agatggacct aaagacattt acagcacaga ttgaactaat gcatcaagaa
     1741 gctttaagac aaagtgtatc gtacgaagac aagtggctca acacgttcca cggcggacgt
     1801 gagagcgcac ttgatcaagt actcaaatta ctgaaaggag aatgtcggga tggataagaa
     1861 agcggcaatg cagcgaatta tcgaattgac ttattcagaa gattggcaaa atgacaaaga
     1921 agctgcttca gaagtgatga ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc
     1981 aagaccacga aaaatcgcaa tttggcacgg tgacaaactt ctagtgatag ggacagctga
     2041 acagttagca agtctcacag gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg
     2101 ctacacagac gctaagaaga gaacgtttag atacgtggag gaatcatcat gacaacagaa
     2161 gaagtgattc aaatgcgaat tcgaagcatt caacgtgaaa ttgacgatct ggaacggaca
     2221 aaggcagtga tggtcaatga aacggcgagg aaggcaatcg atttgcacat agagaattta
     2281 agaagggaaa ttcgtagatt ggaggggtaa ggatggataa gaaagcagca atgaaacgaa
     2341 tcatcgaact gacacattct gagaattggc aagaagacaa agaaatagtt gcagaagtcc
     2401 aaaagctcgg taaatcaatg tggactgaaa agcctaaacg gagaacgccg agaaagattg
     2461 caatctggca tggtgaccga attctagtaa caggtactgc tgaacagtta tctgaaatta
     2521 ctggtct
//
LOCUS       DACECR010000175         2484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.180, whole genome shotgun sequence.
ACCESSION   DACECR010000175 DACECR010000000
VERSION     DACECR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.180"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1015..1305)
                     /locus_tag="IWO17_002795"
     CDS             complement(1015..1305)
                     /locus_tag="IWO17_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291634.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1513..2204)
                     /locus_tag="IWO17_002796"
                     /pseudo
     CDS             complement(1513..2204)
                     /locus_tag="IWO17_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          459 c          348 g          943 t
ORIGIN      
        1 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
       61 gcataaagtg tttagaaaag aacattaaga atagttctat aataattctt gaactccatg
      121 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      181 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      241 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      301 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      361 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      421 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      481 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      541 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      601 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      661 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      721 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
      781 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
      841 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
      901 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
      961 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1021 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1081 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1141 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1201 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1261 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1321 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1381 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1441 actaaaaaaa agaggcagca gtatagatta ctgttgcttc tttactaatt taggtttact
     1501 tgttaagaaa gattatttac gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt
     1561 atcttccact tcatcttttg ctttttctaa cttttgaata tattgtttca agcttgagtt
     1621 ttcagcagtt gcttcgtcta atttcttttg tgtatctgtt aactgatttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2281 ttacatatgt aattttaata taaatactat caaatactca tttaatcctc cttttttatc
     2341 tatttaaaaa atctttttta tagagaaaaa tgcaggttaa tactttctta aataaatatt
     2401 tatggtgaac aaaaacaaaa aagcaagttt tattaaacgt aacccttttc atttcataga
     2461 ttactgtatt tattacaaaa aacg
//
LOCUS       DACECR010000176         2471 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.181, whole genome shotgun sequence.
ACCESSION   DACECR010000176 DACECR010000000
VERSION     DACECR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.181"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..221)
                     /locus_tag="IWO17_002797"
     CDS             complement(<1..221)
                     /locus_tag="IWO17_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291635.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITG"
     gene            complement(218..379)
                     /locus_tag="IWO17_002798"
     CDS             complement(218..379)
                     /locus_tag="IWO17_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0291636.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(394..753)
                     /locus_tag="IWO17_002799"
     CDS             complement(394..753)
                     /locus_tag="IWO17_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ0291637.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            complement(750..1601)
                     /locus_tag="IWO17_002800"
     CDS             complement(750..1601)
                     /locus_tag="IWO17_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0291638.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(1618..2448)
                     /locus_tag="IWO17_002801"
     CDS             complement(1618..2448)
                     /locus_tag="IWO17_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0291639.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
BASE COUNT          590 a          549 c          370 g          962 t
ORIGIN      
        1 aatccagtaa tttcagataa ctgttcagct gtacctgtta ctagaattcg atcaccatgc
       61 cagattgcaa tttttctcgg cgttttccgt ttaggctttt cagtccacat tgatttaccg
      121 agcttttgga cttctgcaac tatttctttg tcttcctgcc aagattctga cttggttaat
      181 tcagcaattc gtttcattgt cgctttctta tccacgctca ttcctccaat ctacgaattt
      241 cccttcttaa gttctctatg tgcaaatcga ttgccttcct cgccgtttca ttgaccatca
      301 ctgcctttgt tcgttccaga tcgtcaatct cacgctgaag gcttcgaata cgcatttgaa
      361 tcacttcttc tgttgtcatg atggaccacc tcgctaaaaa cgctcttcct tgaacgtatt
      421 ccgatatttt ttagctaata tcaacggcgc ttgatattga tgacagaaca actttgcctt
      481 gatcttaaag tcttttgtct gcattccttt gacatctacg actttgacaa gtttgccgtt
      541 tttataaaat gtgaagtcgg gaatatactc gatcttgcga tacttctttc cgtctagttc
      601 aaatttcggc atcagctcaa atctttcctg aagttttact ttccagccat ttgcttcagc
      661 ttgccacaag gctagatcgt aatactctgc ttctgcgata gaatcaaact tgatacctcg
      721 atgaacagtt tttttattac ggtatttatt catgcgatac tacctttcac tggttttatg
      781 cgcttgtctg ctgtttgttg gaatttcagc gcataacctt ctgaattctt aaatatccta
      841 gaaacaattc tttcaccgta ggcttctctt agttcaggac cagataagtt tgttgtgatg
      901 atcgttgcct tgttctgtct ggcttctaag agcgtgttta acgtgttgtt tgtaaactgc
      961 ctactatttg ataccccact acctaattca gctccaatat cgtcaaaaac caccaaatca
     1021 gttgttttaa tatcggctat aagcgatcct tcaatttctt ttctcagttc agtattgtta
     1081 taagaaaact ttatttgctc taataattct tgatagctta taaaaagtat tttcttgtca
     1141 taatttgagc gctcaagtat ttcccaagct gtggccattg ataagtggct ttttccgctt
     1201 cctgatttcc ctgatagaat gaaatgtgca ggatggttca gcagaacctc attcgtatag
     1261 ctttttgctt tttctaaagc gatttttgtt tcttgatcca ctacgtgata attttccatt
     1321 ttgcatttaa acaaagtttt atctgttaat accgaaccat tttgaaaaaa actcaacgct
     1381 cgtgctttta agctatcgtt atatatccgt tcggtctgta tatcctcttt cacacgtaac
     1441 gctttataac cacaactcat gcatgttggt ttacaacgtt ctgaaccatc cttattttta
     1501 gctcgccaac tatacaaagg ttcgctacat tctggacatt ttccgctttg cactaatact
     1561 cttcttatta gtttctccat agcatttgct aggctttcca tgtgatgcat ctccttttta
     1621 aattggcaag tcgtcatatt cactaggatt gctgtactgt agtttttgac tttgcttttt
     1681 atgattcttc ttgtctgctt tgatttcgaa tttgagcttc tcaaattttt ctctcaattt
     1741 cttagcactt ctaatatttc caaaccaaaa ttcatttgta ggtagccaat tgatcacata
     1801 ctcaatcgct tctatagatg ctttatctct ttcttccatc aacctgattg tgtctgccca
     1861 tttttcgata tctactttgt tcatttcttt tggaaaatct tcagttaaat tactttgcaa
     1921 ctttttagca aggcgtaagt gttcgtcaga atacttacct ttcttttctt ctttatctat
     1981 atctttatct tcttctatat ctttatctgt accgtcacgt gacgtcacgc taacgtcatt
     2041 ttccaatttg agacgttcct gtctctttct ttccctgtat ttacggtttc tttcagcatt
     2101 ttttagcctt actttatcca taccctcgat attttgatgt ttttcccaat tactgatggc
     2161 aattagtcca tcactgctta gatcaatcat gttgaaattt gccaatgtag ttagcgctaa
     2221 gcgaaccgta tttacgtttt tgccaaacaa tgtagcaagc atttcttcgg tataaggcat
     2281 gttcctctgg atatatatca gaccatcgtc gttagtcttt cctgctaaaa ctagtaatcg
     2341 aatccatata acgatgatgg catccgactc aggaacagct tggattaatc gtattttttc
     2401 atcgtcaaac atagtagttt taagtttgat ccaacttatc tcagccaaat ttatcctcct
     2461 atccttaact t
//
LOCUS       DACECR010000177         2418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.182, whole genome shotgun sequence.
ACCESSION   DACECR010000177 DACECR010000000
VERSION     DACECR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.182"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            5..1149
                     /locus_tag="IWO17_002802"
                     /pseudo
     CDS             5..1149
                     /locus_tag="IWO17_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="IWO17_002803"
     CDS             1320..1778
                     /locus_tag="IWO17_002803"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ0291640.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..2407
                     /locus_tag="IWO17_002804"
     CDS             1937..2407
                     /locus_tag="IWO17_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ0291641.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          810 a          454 c          455 g          699 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctttaactt aataaaaa
//
LOCUS       DACECR010000178         2406 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.183, whole genome shotgun sequence.
ACCESSION   DACECR010000178 DACECR010000000
VERSION     DACECR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.183"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..103)
                     /locus_tag="IWO17_002805"
     CDS             complement(<1..103)
                     /locus_tag="IWO17_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0291642.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
     gene            complement(177..458)
                     /locus_tag="IWO17_002806"
                     /pseudo
     CDS             complement(177..458)
                     /locus_tag="IWO17_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            920..2302
                     /locus_tag="IWO17_002807"
                     /pseudo
     CDS             920..2302
                     /locus_tag="IWO17_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          875 a          412 c          400 g          719 t
ORIGIN      
        1 acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa
       61 taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt
      121 cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac agaaccgaaa
      181 tgagtatagt atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa
      241 ttgatatctc gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat
      301 ggctcagaaa gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat
      361 atttcgattt ttctttcata ataagatctg ttttggtcct caaatttagc caataactca
      421 aattgattta ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt
      481 tcattaacaa aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg
      541 tgaacacgaa ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc
      601 atacgttctg gactatttgc taatagatta aaatttcagc atctatttct gaccataaat
      661 aaatgagctg ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaacttttaa
      721 gtttctgacc ccgtaactca cacttcaaaa tcttacttaa aaaggagtct gaaagaaaaa
      781 aagagtattc aaaatttttt tctaactagc tacatcacat ctatgcattg acactcaaag
      841 aaccagaata agtcatcaga aagcaagggg gtgcggacat aatcctggac gaaaaaataa
      901 acttaggagg aatgtccgtg tgttttccca caaagagcgt gtaagggcaa ttcagttatt
      961 tttaaaatac gattgctctt atgctgcaac cattcgagaa ttagggtatc cgtcgattgg
     1021 tgcactacgg aaatggtata aagagtattt ggaatccgga gagctccatc aggagcaccg
     1081 aaaaaaatca aagtattctg aagaacaaaa acgaatagcg gtgaatcatt attttgaata
     1141 tggtcaatgt tatgcacgaa ccattcgcat gcttggttat cctaatagag agtccttgcg
     1201 gcagcggtgt gaagaacttg caccaggagc tagaaaactc cgaaaaagtg cggtaaagtt
     1261 aacacaagat caaaaagaag ctgtgttgaa aaaattctat aaaccgcaaa ctaataggaa
     1321 aaatttagcc gaatctggag gaattagtag agttactcta tatcaatgga aaaacacggt
     1381 tcttggaaag gattttccat tgtgtatgac tttaaaaaat caggaaaaac aaaaagagct
     1441 acttttaaag gaagtagaag acttacaaaa acaagtacac caattacagc tcgaaaaagc
     1501 attactcgaa ggagcggctg aactgctaaa aaaagaaaaa ggcgtcaatc ttctttgtct
     1561 atcaaatcaa gaaaaaacga tattgattga tgccttacga aatcagttca ccttgaaaga
     1621 gcttcttcaa cagctccagt ttcctaaaag cagctatttt tatcaaaaac aagcacttga
     1681 gaaaccagat aagtattaca aagaacgtca aattaattat cacgattttc aatcataact
     1741 tctgtgcgta tggttaccgt agaattcacc aagctttaaa aaatatgggt aaaaagcttt
     1801 ctgaaaaagt tgtacgtaga ttgatgactg aggaaaatct atttgttaaa ttttctagac
     1861 gaaaaaagta ttcttcgtat gctggggaaa tttctcctgc acaccccaat ttattaaatc
     1921 ggaattttaa agcaaagaag ccaaatgaaa aatggttaac agatattact gaatttagaa
     1981 ttctcgcagg gaaaatctac ttatcaccac ttgtcgattg ttacgatggt gctattgtta
     2041 gttggactat tggaacaaag ccaacttgct tcactctatg tctaaaaaga gttattcacc
     2101 agataattca gcgtgcgaag gtttctttgg acgattaaaa aacgagatgt tttatcaacg
     2161 tgactggaag aatacaacaa tcaatcagtt tataaacaaa ttggatgatt atattcattg
     2221 gtataataat caacgaatta agttatcttt aggaaggcta agccctcttc aatatcggaa
     2281 gaagcaaaga tatatttgtt aaaaaggtcc aaaaaaaata tccgcacccc cttttttttg
     2341 taaaaaacta ttgcacaata caagtagcct acggctatcg aaatttccat aactttcgag
     2401 caagaa
//
LOCUS       DACECR010000179         2397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.184, whole genome shotgun sequence.
ACCESSION   DACECR010000179 DACECR010000000
VERSION     DACECR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.184"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            206..2068
                     /locus_tag="IWO17_002808"
     CDS             206..2068
                     /locus_tag="IWO17_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0291643.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="IWO17_002809"
     CDS             2068..>2397
                     /locus_tag="IWO17_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0291644.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DACECR010000180         2350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.186, whole genome shotgun sequence.
ACCESSION   DACECR010000180 DACECR010000000
VERSION     DACECR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.186"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..543
                     /locus_tag="IWO17_002810"
     CDS             <1..543
                     /locus_tag="IWO17_002810"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0291645.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1016..1588)
                     /locus_tag="IWO17_002811"
     CDS             complement(1016..1588)
                     /locus_tag="IWO17_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0291646.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1604..2209)
                     /locus_tag="IWO17_002812"
     CDS             complement(1604..2209)
                     /locus_tag="IWO17_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ0291647.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          741 a          467 c          359 g          783 t
ORIGIN      
        1 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
       61 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
      121 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
      181 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
      241 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
      301 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
      361 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
      421 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
      481 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
      541 taaccgtctc tactaaaatg tgtccaagat attgactcaa atccagtaat atcacaatag
      601 gaatgctgtt tattatatta gcagcagtgc tgatttttat gatagttatt tggttcttaa
      661 atgaaagatc taaagacaat caacaccgta gggatatgga gataaaatca atagagtcaa
      721 tagattattt atcgataata acactaaaaa agccacgttc ctaaacgtga cttacgctga
      781 acgatatgtg ttgtttggta gacgaacaaa tcgttcatta taaatatatg atggtatcct
      841 aatatagtaa gcgtggctag cataccttta attactttac cttttacagt taccatacac
      901 attttttgag cagaaatcaa gtaaataaaa aaatattata tttcacaaca tgctaataga
      961 ctcatatcta gaacgcatta agtatttacc tgtctggttc gctgagactt cttttttaac
     1021 tttcttcaat tttcttcttt attctaaaaa gcgtactgcg actgaaaccg gttatagctt
     1081 caacttcctt atacgattta cctgaagtaa gcagttcata tgcgtgcctc ttcttcggtg
     1141 tgattgttgc tttcggacgt ccttctctga aattaggatt attctttctt gcaaacattt
     1201 ttccttcttg ggtacgttcc acaatcatat cacgttccat ttctgctaca ctcaacaacg
     1261 tccgtgtcac cattcgcccc atggacgtat tttcaatcgt tcctagattc aaaaccttga
     1321 tcgttacgtt atcatcgagt aaaggttcaa tgatctctaa tgcttctctc gtgttacgag
     1381 caaatcgatc aagcttcgta acaactattt catctccagg cttcagacca gccaacagct
     1441 gattgaattt tggccgatcc cgttttgtac ccgtaaattt ttctgaaaaa atattcttct
     1501 tctctacgca gttctcaagc aactgttgaa tttgttcttt tgtactttga gaagcggttg
     1561 agacacgtgc atatccatat ttcgtcattt ggtccaccta cttttacaaa tcatctatcg
     1621 tatactcaga atacccttca tagaaatagc tgacatcgat tcctttcata tataactcgc
     1681 gatcgtcaat tttatcagtc agagcattgg aaattagata gcgtatttcc aaatcgttta
     1741 tgggacttcg ctccattgca gaaagatagt ctgctttatc gattaagttc caatctacaa
     1801 ctttttgcaa ttcttccttt aaaatgagat ccagccaaat tctagtactt cggccatttc
     1861 cttctcgaaa tgggtgagcg atattcatct ccacatattt cttgataatc tcttcaaaag
     1921 aagtttgtgg catctgatca atatgctgta acgaatgttc taaatacata actggtgcaa
     1981 atcgaaaatt tccttttgca atattgaccg atcggatttt tccggcaaaa tcgtaaatat
     2041 ccgaaaatag aaattcatga atttcagata aacctttaaa tgtaccaact tcgatttcat
     2101 ttatttttcc acaatcgtac aattctttcg ctttttgttt actgagtttt tcttcaacct
     2161 tagccaactc aacttgattg gttaatccca atttattctc taaaatcatg cgaaatctcc
     2221 ttaactttat gacataagtt tatgaaacac tctcaacccc attatatcgc gattttgttt
     2281 atgtgtcaaa aactattaag tttctgaccc ccgatagatt tttcaagtgt ctctattttg
     2341 cacagtctag
//
LOCUS       DACECR010000181         2340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.187, whole genome shotgun sequence.
ACCESSION   DACECR010000181 DACECR010000000
VERSION     DACECR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.187"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            133..345
                     /locus_tag="IWO17_002813"
     CDS             133..345
                     /locus_tag="IWO17_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291648.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            507..1025
                     /locus_tag="IWO17_002814"
     CDS             507..1025
                     /locus_tag="IWO17_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAQ0291649.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1032..1544
                     /locus_tag="IWO17_002815"
     CDS             1032..1544
                     /locus_tag="IWO17_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291650.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1815..>2340
                     /locus_tag="IWO17_002816"
     CDS             1815..>2340
                     /locus_tag="IWO17_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0291651.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIV"
BASE COUNT          892 a          298 c          385 g          765 t
ORIGIN      
        1 aaaaacacta tatattccga aaagtgagta tctccattca taaaataatc aacatattta
       61 aaaaatctaa ttagcttttt tagtcaatct aaagttcttt attctatgat ataatgttac
      121 tgtagaaagt ttgtggcggt ttggcaaatc caattgaagg agcgtggtgc ctatgggctt
      181 attagcttat cggacgtact ctgtagaaag gaaatgcctg atttgttctc attggatcaa
      241 atcattgcta ttatcggagc agcagcagta gttattactg ccgtagcgaa agctagcgat
      301 tcgattttag cttggttaaa gttcaaaaaa gaacaaaaaa aatagagccg tctcaacttt
      361 tggcgaggta gacgagctct aaagctttat ttacatgcca tccgctttga acggactaca
      421 ctgggcatgt gacagcatgc cctttcattt atattatatc tcattctttt atataatgaa
      481 agtaaataaa tcatggagga taaaatatgg ataaatttaa tagattaaca ttaataaatc
      541 agtttgaaat tttaaaagct ttaaatccta acgaagaaaa atactatgct gaaaaaatag
      601 aaatactaac agaaggttac gaatatcact atagtgaaat atttgaaaat atttctgatc
      661 cattgccaga agaagattcg cggtttgttc tagacatatt aaatatgtat agagatatta
      721 cttttagtaa aaataaatta aaagatataa attcaattga taattattat atacaattta
      781 aaggttttga ctttaatagc tctaccgaat ttaagttagc actttatgca caatttttta
      841 ttgaaaaatt aaataggttt caagaaatag ttgaagatag tgattttaac acttttaata
      901 gccataaacc gatgagaggt acttatataa aatttctcga aaattataat gatttaaaat
      961 ctaccaataa ttatcaattt ggaaacttgt cttatgagat gataagtaaa gttctaagtt
     1021 tgtgatgata aatgaaatta aatagtataa atgatttatt tgctattata ggtgttattg
     1081 ctaacatact tttttcgtgt ttgaatttta gatttactca aaagaacaaa aagaacaaac
     1141 tcagtataga acaaatgaaa aaagacattg aaactgattt aaaaaatgac cataaagaaa
     1201 gaagaaaaat tgaagaatat acttctttaa tggaaaagtg taaagcaact gatgcaattt
     1261 ttagcttttt aagaaaaagt gatgagataa tcacacgtga aaatatagat ttagaaaatg
     1321 agattagtaa acttgaatct atcttttccg atataaattc tcttaagtat ttaatcgtcg
     1381 aagaaaattc ggatagcttt ttagaatttg aaaaatatct aacagatctt atagataata
     1441 gaaataatga attaatgaga caaaacattg caagattcct tactaaatta gtttctgaat
     1501 ggaaaagtat tatttctcaa aatatggata aattaagata ttaaggatgt tagttttaaa
     1561 ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat acttaaagga
     1621 ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta tttgttgttt
     1681 cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga tttttcagcg
     1741 gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa tagattggat
     1801 aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat caaagtttag
     1861 ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct gaagacgatt
     1921 taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca gagctgcaag
     1981 agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt gatcgattag
     2041 caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa ggtgcttcta
     2101 ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta agagacttgc
     2161 taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg aaaaaaatca
     2221 aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat actggtaaaa
     2281 agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat gaaattgttg
//
LOCUS       DACECR010000182         2310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.188, whole genome shotgun sequence.
ACCESSION   DACECR010000182 DACECR010000000
VERSION     DACECR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.188"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            25..702
                     /locus_tag="IWO17_002817"
     CDS             25..702
                     /locus_tag="IWO17_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0291652.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
     gene            complement(839..1255)
                     /locus_tag="IWO17_002818"
     CDS             complement(839..1255)
                     /locus_tag="IWO17_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291653.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1258..1542)
                     /locus_tag="IWO17_002819"
     CDS             complement(1258..1542)
                     /locus_tag="IWO17_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291654.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(2242..>2310)
                     /locus_tag="IWO17_002820"
     CDS             complement(2242..>2310)
                     /locus_tag="IWO17_002820"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0291655.1"
                     /translation="CSLLVGLLSALFSYWLNNRNKK"
BASE COUNT          747 a          355 c          416 g          792 t
ORIGIN      
        1 ttaacagtca aatatcaaaa tgaattgaat ttagtaccgc tgaaaaattt caatgctaag
       61 cagatggatt tattttttgc attatgcgct cgtatgaaag atcgtggttt gaaaaaagtt
      121 agattttctt tcgaggaatt aaaagagtta agtgattata agatgactgc tacaaaagcc
      181 tttgtcggtg atttagaaaa gctctataaa gatatgctta atcttactta ccgtactgaa
      241 gatgaggatt ctattcgcta ctttgttttg tttactggtt ttgaaatcaa taaaacaaaa
      301 aagtttgttg aagtcagtgt taatccggac ctagaatata ttattaatgg tttaacagct
      361 gaatttagta gatttgaatt atctgctttc acgtcgttgc gttctacgta tgcaaaaact
      421 ctattccgac tgcttatgca gtaccgttct actggttatt atgtagttcg tattgaagag
      481 tttaggagct taatggatat cccagaatac tatcaaatgg gtaacattga tcaaaaagta
      541 ttaaatcctg cattacgaga actaaaaaaa tacttcaatg atcttaaagt cacaaaaata
      601 aaagcaaaaa agggcaataa aatcgccaaa ttagaattta ctttttcaag cctaacgtca
      661 gatttgccaa agatttctct gcgtaactgg ttagaagaat aaataaaaaa gaactcgccc
      721 cccttacatt aagcaagtag acaagtccct tacaaccgac taagattgct agtacattct
      781 agcatattgt atggggcttc taaataaaaa acagatagga ataactccta tctgtttttt
      841 atcgttttag aaaatttgtt gggtcgaaat ttctagaagt gactgtttga ctggtttctt
      901 tttgatgatt ctttggtcga tttcgatttg atggatttag tttagcttcg attttttctt
      961 tcacttcttg atatacttta gaatttgtgc caataagaac gcctgtatct tttagatata
     1021 aatcagcaaa ttctgcgatg atttcagtca tgatggaaat ttcttttccc atagcattta
     1081 gttttgcctg attgctggta gtggtcgtgt tggtatcggg ttcataactc atacataaaa
     1141 agcgaatggc ttccgagtag ttattgatat ctggattttt ttcgatgatg gaatctaaaa
     1201 cgtcaacatg actagggtac aaagtaacat gtttttggac agaagttttt ctcatggtta
     1261 tatcattcct ccgataagca tttcaatgat ttgattattt gtgtagacat actcgcttaa
     1321 tatttgagta ttttctgttt ggatagcaat aaatttatcg agcaataaat cgaattggtc
     1381 ttttttgtaa gtcgtaagtt gacggtcaaa atcactttcg attaaaactt ttagatattt
     1441 atttcgactc catttttctc ctgttttatt cgtgagttct tggcaacgtt tgtctattgt
     1501 tgcaacaaac tttggatcaa tattccgaac taaaatgtcc ataaaaaatc tccttttgca
     1561 attatgtata atttatgtat accatacaaa tactatattt gtatagctgt tttttgccaa
     1621 aatgctatac ataagctacc cacgatggcg aaaaagtgat atcaaatttt tttctgagaa
     1681 acgtgatagg cacagcctat ctacccactt tttttagaaa gtgggtacac gtttccttat
     1741 gctctttaaa attcgggggg gagtgctgca cgtgtaatcg ataaccaaat ttaacagacg
     1801 aaaaattgtt ggaatataaa aatatgacac ccgaaagaaa acgatcgttg gaaaaattaa
     1861 tgcaactagt gaacgaagac ggcggaattt cagacgcagc ttttgacgca tggagcgaat
     1921 ttcatggaga atataattaa gtgggtttaa atggctctaa aatcgcttct aagagttttt
     1981 tagcttggac caatgaaata caccaaaaac taaataaaac gtcttaaatc gaaaaatacg
     2041 agctctcaga gcgactttat ttgtaaatgt tgcatataag aaataattac ggattttaga
     2101 cttttgtagg atatttttga aataaatatt tatttctgtt ataatttgat tgcaaataag
     2161 tgaacacgaa acacacgaaa tccccagaga ggtcggattc tctggggctt tttttgtggg
     2221 ctgagtcgcc ttaattaagt tttacttctt gtttcggtta ttcaaccagt aactgaacaa
     2281 agcgcttaaa agcccaacaa gaagggagca
//
LOCUS       DACECR010000183         2259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.190, whole genome shotgun sequence.
ACCESSION   DACECR010000183 DACECR010000000
VERSION     DACECR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.190"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(148..2145)
                     /locus_tag="IWO17_002821"
     CDS             complement(148..2145)
                     /locus_tag="IWO17_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ0291656.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          574 a          505 c          411 g          769 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaa
//
LOCUS       DACECR010000184         2120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.191, whole genome shotgun sequence.
ACCESSION   DACECR010000184 DACECR010000000
VERSION     DACECR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.191"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..225)
                     /locus_tag="IWO17_002822"
     CDS             complement(<1..225)
                     /locus_tag="IWO17_002822"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734812.1"
                     /note="malic enzyme; oxaloacetate-decarboxylating;
                     NAD-dependent; catalyzes the formation of pyruvate form
                     malate; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ0291657.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLF"
     gene            complement(666..1985)
                     /locus_tag="IWO17_002823"
     CDS             complement(666..1985)
                     /locus_tag="IWO17_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ0291658.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          651 a          364 c          413 g          692 t
ORIGIN      
        1 aaacaatgtc cggttcgtat tgaatagatt cattaagaag atacgttttt ccaaatcggt
       61 ctgtttgctt aagtattgac cgtaagcttg gacagattgt tgttccaatg tttggacagt
      121 acttggaagc ataccagtga ttccgtattt tgcccgttct tcttttgtaa aagctgttcc
      181 tttatttaga aaaggattat ttaataagtt tagccctgta agcatgaatg attcctccta
      241 agttttaata caaacgaagt ataggagtca gatgaggata tagtcaaaaa gagcaggtat
      301 aataaatttt atttatatga gtaatagagt caaaagaaat caaaatagcg gtatttgttc
      361 tttttatatc aacaatcctt gcttgattac tatgcgtatc tcttatatgc taaagaaaga
      421 aaagtagaag aataaaggat atgttagata gataaataga atcctgaata attcatagct
      481 ttaattcttt ggcacatttt attgaaatat gtatcactaa tatccctatc aactgatttt
      541 gactaaaaag ttacttcaaa cattcactgt tcaaaaatat tgaggaataa attttgggtg
      601 taaggcattt tgagcatact tctccctgga taacttccca gtaaaaaaat cgttagattg
      661 ttggattaga tccattgact ggctcgcctc aggcgagcaa agaccctgta gaattcattc
      721 tgatacacat gataactaga taatttgaga tagactcgtc tacccgtttg gactaatttc
      781 ccagcaactt tcagaaactt caatcgaata gaatgaatgg tcattccctt ttggacttct
      841 tcaaagccaa tcgttcttaa gaagttgact aagttgtaag ctatcaagct gagcatcatt
      901 cggacatgat tctccaaaaa gcgaggactg tctgttttgt caaagtaaaa gccagctttc
      961 gcttctttaa tgaagttctc cattgtgcca cgtttggcat agatagaaaa gatggtatca
     1021 ggagaaacat tttctgatag attcgtcacg ataaattcat gtcgaaagag tagttcgccc
     1081 gcttcacgtg ttgagcgtat acacacgcga cgactttgtg accaggattg tgcttgataa
     1141 gtggtcgaga agtactgaac ttctcgttct tcccactttt gattatcgcc ataaagtact
     1201 gatttctcag ctatttgacc tagtctacga ttattcttca gtcgaataac ataatgactc
     1261 ttttttgatt cacacgaatc atacacacca ggtgtagcga acccgctgtc tccacgaacc
     1321 aagatgtcag tgtttggtag agagtgatta tagtgctcta ataaaggtgt gagaaactcc
     1381 tttacgcctt ttgatgtata ttgatttcct gaacgtagtt cagcttttaa gaagtcacca
     1441 gtcaatccgt caaaagctac caatggatgg taaccatagg tttggtagtg tgtattataa
     1501 ttcgtttgtt cttggtgacc aaatgtatct gaatgggtcg aatctaaatc aatgattaat
     1561 tccgtatcat tacggatgag gcgtgcttta tcgataagtt cttggttcaa agcttgaagt
     1621 tcatggatat tctcctcaga tagtcgatct aaaaatcggg aaagtgaaga ttgagaagcg
     1681 agttctttcc tatctaaaac agctttaaac acaggatctt gtctcaggag attagctgca
     1741 gaatcagctg agtaaccagc aattaattgc ataatgaact gctcaagtat ggataagttg
     1801 tcatgagtaa aatacgctcg ttcgtcttca atgtgaatgt gttgcttagc cagttcagaa
     1861 aaaccaaaga tgttcatcag ctctttcact aggacgagac ccgaatcact cgataattga
     1921 ccacctgtat gagaaatagt gatatttgaa ttgaatttta cttggttttt gtgtaagcta
     1981 gtcatgaaaa gaactccttt cttttggttg gtttagtcac tttaaccata gcagaaaggg
     2041 gttctttttt catcacttaa cgggtgaaga tgaaaaagta attgtaagct gccgtgaggc
     2101 agtttcacat ggttttaatt
//
LOCUS       DACECR010000185         2046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.192, whole genome shotgun sequence.
ACCESSION   DACECR010000185 DACECR010000000
VERSION     DACECR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.192"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..605
                     /locus_tag="IWO17_002824"
     CDS             <1..605
                     /locus_tag="IWO17_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0291659.1"
                     /translation="PKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVD
                     KKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEA
                     GTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQS
                     SKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            739..>2046
                     /locus_tag="IWO17_002825"
     CDS             739..>2046
                     /locus_tag="IWO17_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0291660.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFI"
BASE COUNT          798 a          254 c          375 g          619 t
ORIGIN      
        1 taccaaaagg aactacttat acttggaaag aaaaagtaga tgttagtaca gctggaaaca
       61 aaaaaggtac tgtagtagta acatatccag atggatctaa agaagaagta gaagttacta
      121 tttctgtagt agataaaaaa gcaccgaata aacctcaagt tgatcctatt acagagggtg
      181 accaaattgt tactggtaaa actgaaccaa atgcagaggt gacagttaca ttaccagacg
      241 gaagtcaaca ccatgggaca gctgataaaa atggtaattt tacagttaaa gttcctaaat
      301 tagaggcagg gacaaaagtg atagtaactg caactgatga atctggtaat actagtgaac
      361 ctactaatgt agttgtttct tccaacaaaa aagacagtgg aaaaaacggt agtaaaggta
      421 gtaagactga caatcaaggt agcaacagca atcaagacaa aaatagaggc aaaagccagt
      481 cctcaaaagt ttttcctaag acgggagaga gtgacagtaa tatctttact attagcggtg
      541 gtttaatact gttaggaacg ttaggattat tagggtataa aaatcgaaaa aaagagaatg
      601 aatagaaaat caatttctta agtcttctct gagaagagga agaatctttt tcttcctctt
      661 tttgcttttt tatgatggga aataagtgtt agttaaagat gaatatactt tttatagaaa
      721 ggaaaagaat gtaattaagt ggaaaaaata gtaattattg gttcaaacca ttcagggcta
      781 gccgcagctc gatttctaaa cttaagcgat tcaaattatg aagttattct aatcgataaa
      841 aaatcagaaa ttagttactt ggaatgtggg acatcgttat ttataaaagg agaagtaagt
      901 aatttagaag atctttttta tcaaaataga atgacactaa attctgagat aaaaaagtta
      961 tttctagaga cagaagttga caaaattgat attcaaaata aaaagattag ttgtgtaaaa
     1021 aaagatggta caagattctc tcaagaatat gacaaagtta tattagctac aggttcaaag
     1081 caaattatgc tagatatacc ggggaataat ttagctaata ttttttcgat aaagaatttt
     1141 ccaaatgcaa aagagatttt taaagctttg gatgatagta ctattaaaaa agtaggaatt
     1201 attggagcag gatatattgg agttgaaatt gctgaagcca taagaaaaag agggaaagaa
     1261 gtatatcttt tcgatgttgc agatagagtg ttgtcgactt attacgaccg tagtttttca
     1321 gataaagttg aagaaatact atcaaaaaat ggaatacatc tttgtctatc agaaagtcct
     1381 aaaaaatatt taggaagaaa aaagattgaa aaaatagtca ctacaagtgg tacccaatac
     1441 tctatggata tggtcgtaca agctatagga tttctatcta atagtccaat tggggaaaag
     1501 gaactcttga gagatgttag tggagcatat ctaactaatg aataccagca aacaaatata
     1561 aaagatatat atgctattgg ggattgtgct acaacttttt ttacgtccat taatagaagt
     1621 agtgttgatt tttctatttc taatgctcta agaacagctt atattgcaac tcaacatatt
     1681 aacaatcaag attttacacc ttctggttcc caatttacaa atggcttttc tatatttaat
     1741 tacaattttt acgcagcagg tttgaattta aaaacagcac gattattaag tgagtctata
     1801 gattatatag aaatagaaga tttcattaga cctgcttttt tgagaaaaaa tagtcgtgta
     1861 aaattaagaa ttagctttga aaaaaataca aagagaatag taggtgtaca actttgtagt
     1921 caagaagatt tatctggact attatcaatg ttttctcttg ctatagaaga acaaattaca
     1981 ttagacaaat tgaaaatgtt agattattta tttcatccta gttttagcca accctataat
     2041 tttatt
//
LOCUS       DACECR010000186         2034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.193, whole genome shotgun sequence.
ACCESSION   DACECR010000186 DACECR010000000
VERSION     DACECR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.193"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IWO17_002826"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IWO17_002826"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ0291661.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IWO17_002827"
     CDS             complement(1431..1823)
                     /locus_tag="IWO17_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0291662.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          452 c          342 g          662 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tact
//
LOCUS       DACECR010000187         2020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.194, whole genome shotgun sequence.
ACCESSION   DACECR010000187 DACECR010000000
VERSION     DACECR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.194"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            100..570
                     /locus_tag="IWO17_002828"
     CDS             100..570
                     /locus_tag="IWO17_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ0291663.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="IWO17_002829"
     CDS             792..1895
                     /locus_tag="IWO17_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0291664.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          546 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatact
//
LOCUS       DACECR010000188         2006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.195, whole genome shotgun sequence.
ACCESSION   DACECR010000188 DACECR010000000
VERSION     DACECR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.195"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(104..874)
                     /locus_tag="IWO17_002830"
     CDS             complement(104..874)
                     /locus_tag="IWO17_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0291665.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(867..1802)
                     /locus_tag="IWO17_002831"
     CDS             complement(867..1802)
                     /locus_tag="IWO17_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0291666.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
BASE COUNT          621 a          458 c          359 g          568 t
ORIGIN      
        1 ctgaccctcg gtattagctc aacaaacgac aaaatatgag gaactatttt cccgtttgtt
       61 tctctaatac tcaggtcaaa acgctgattt cacaacctct tttctaactt ctcaatcctc
      121 ttcctttatt gatcaaatac caagtaacgg cgtacaaaac aacgatgaat agcacaagta
      181 cgatcatcga tgttgctact ggcacatcga ttgttccaag gaatccgtaa cggaaaccga
      241 agatcatata aacgatcgga ttgactttcg aaacagcttg ccaaaatggc ggcaacatag
      301 agatggcata gaaaacgcca cctagataag ttaacggctg caagacaaat gtaggcacga
      361 tcgatacatc gtcataagac tgagcaaaaa tcccgttgag cagtcctgct aacgaaaaca
      421 agattgccgt catcaataat gtaatgatga ccatgaacca tgaatacaca tgaagtggta
      481 caaagaacaa ggaaatgatc gttacgagag agccgacgag aatactgcga cccactccgc
      541 caatcacaaa gccccaaatg atcacatgcg taggaacagg cgctaccaag atttcttcga
      601 tgtttttctg gaatttttga gagaagaagg acgaagaaac gttggcatac gaactagtga
      661 tagcagacat cataatcaaa ccaggcacga tgaattccat atatgaaaaa ccacccatgt
      721 caccgatccg accaccgatc atttttccga aaataacaaa gtacaaggat gtcgtgatca
      781 caggcggaac cagtgtttgg acccagatac gcaaatagcg gttcgtttcc ttgatagcta
      841 aactttttaa tgcagtaaaa taaagattaa acatttttct cctccgtatg attattttct
      901 tctgtgattt tcaaaaacag ttcttctaaa cgattcgact tattcctcat agaaagaact
      961 ttgattcctt gtcttgtcag ctgatcgaag attccgttca ccccttgatt tcgttcaact
     1021 tcgacagcga ttgtctgttc atcttcaaat gtacaagcgt atccttcaat cacaggtttg
     1081 gtctcatatg gcgctaaatc aaagataaat gtttcaaatt gcagtttgga tagaagctct
     1141 ttcatactag tattctcgat cagttcacct gattggatga tcccgatgtt gcgacaaagc
     1201 atttcagctt cttctaaata atgcgtcgtc aaaatgatcg ttgtaccatt ttcgttcaat
     1261 tctcggagaa actcccacat ctcgcgtctc aattcaatat cgactcctgc tgttggttca
     1321 tccaagatca gcaaacgtgg ttcatgcatc aatgccctag caatcatcag tcttcgcttc
     1381 attcccccgg aaagcatacg tgcacggaca tttctctttt cccaaagatt ggactgcttc
     1441 aaatattttt cgctgcgttt catcgcttct ttgcgagaga caccgtagta gcctgcttga
     1501 ttcacgacga tttgctggac cgtttcaaag ggattgaaat tgaactcttg cgggactaat
     1561 ccaatctgtt gctttgctcg gactagatcc gtatccagat cataaccgaa gacattcact
     1621 tttccagatg ttttattgac taaagaagta atgattccga tcgttgtaga cttcccagct
     1681 ccatttggtc ctaatagcgc atagaaatct ccttctttca cggaaagatc gattcctcgc
     1741 aatgcttcga ccccacttcc ataaaccttt ttcaaattat ttatttcaag cgcatttgcc
     1801 atttatttcc tatctcctta tagacatgat tctttattgt atcatggaat acagcagatg
     1861 aaaaaacaac ttggttccag cgaaaaatca ggttgctggg ctcaggaaaa tctcctaatg
     1921 aaagacatca atcggacgaa gtcagacaac cttttcagaa aaaacaaatc gaagataaag
     1981 gattactatt catatgtgca acaaaa
//
LOCUS       DACECR010000189         2003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.196, whole genome shotgun sequence.
ACCESSION   DACECR010000189 DACECR010000000
VERSION     DACECR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.196"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IWO17_002832"
     CDS             <1..378
                     /locus_tag="IWO17_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ0291667.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IWO17_002833"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IWO17_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0291668.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="IWO17_002834"
     CDS             1859..>2003
                     /locus_tag="IWO17_002834"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0291669.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DACECR010000190         1961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.197, whole genome shotgun sequence.
ACCESSION   DACECR010000190 DACECR010000000
VERSION     DACECR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.197"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            238..558
                     /locus_tag="IWO17_002835"
     CDS             238..558
                     /locus_tag="IWO17_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0291670.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            584..901
                     /locus_tag="IWO17_002836"
     CDS             584..901
                     /locus_tag="IWO17_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0291671.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            930..>1961
                     /locus_tag="IWO17_002837"
     CDS             930..>1961
                     /locus_tag="IWO17_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0291672.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVL"
BASE COUNT          608 a          337 c          409 g          607 t
ORIGIN      
        1 accgtttatt tgaaaaaagt tgtgacaagt tttgtgtcac aacttttttt gtgcacgtta
       61 aaagaaaaaa ggtaataaaa attaatttgt gtgtttagtg tgcgctttct ttttttagtg
      121 tgtatgcaac ttggtataga ccttaaatgt gatgatatca actttttaaa gcgcttttaa
      181 agttggcacg ctacttgctt atatacaagt gtaagtatag aaaggaacgt gtttaaaatg
      241 gaaaagaaaa caatcatgct tgtatgttca gctggaatga gtacaagtct cctagtcaca
      301 aaaatgcaaa aggcagcaga agcaaaagga atggaagcag acatctttgc tgtatctgct
      361 tcagatgcgg ataatcaact agaatctaaa aatgtagacg ttttactttt aggaccgcaa
      421 gtacgcttta tgcaaaatca atttgctgaa aagctagcac ctaaaggcat tcctttagat
      481 gtaatcaata tggctgacta tggcatgatg aatggtgaaa aagtactcga ccaagcagaa
      541 aaactaataa acaaataata aaaattatcg gaggatacaa taaatggaag atcaaaaaaa
      601 tctagaagcc atcatgggtc tgatcatgtt tggcggaaat gcaaaaagcg atgcaatgga
      661 agcgattgct gctgcgaaaa aaggtgattt cactttagca gatgaaaaaa tcaaagatgc
      721 agaggaatct ttagtccaag cacatcactc tcaaacagag atgctgacac aagaagcaca
      781 aggaaatcac gttgaagtca cgcttttgac ggtccacagt caagatcatc tgatgacatc
      841 tatagctttt acagatttag caaaagagat cattgatctg tatcgtcgat tgaacgcgta
      901 atctacagta aagaaaagag ggaagattta tggataaatt tgttgcattt atggagaaac
      961 attttatacc tgtagcatca aaaattggtg cacaacggca tttagttgcg attcgggact
     1021 catttatggt gagtatgccg ttaatgatct taggagcatt agcagtaatg atcaataact
     1081 taccgattcc cgggttccaa gaattgatga actcaatttt tggtggtgaa tcatggaaag
     1141 gattcggggc tgctgcttgg aacggtacat ttgccattct ttctgtcttg attgctttct
     1201 tgcttgctta tcatttagct aacggctacc gaaaagacgg tgtttccgca ggtgtgattt
     1261 ctttaggctc tttcttcgct ttaggcggag cattaggtat gagctcaaac ggtttgttta
     1321 ttgctattat tgttgggatt atttcaacag aaatctttgt acgtttaagc ggaaatgaaa
     1381 aattgatcat caaaatgcct gatggtgtgc caccggcagt tgcaaaagca tttgcttcat
     1441 tactaccagc aatgatcact atctctgcgt ttgctttagt tgctgcaatt tttgctgcat
     1501 tcggtgttga tgatatcgta ggttctttct atacagtggt ccaagaacca tttatgggac
     1561 ttgcaaactc ttatccatct gccttactgc ttgcatttat cacaccattc ttatggttct
     1621 ttggtttaca cggagcgaat atggtcgatc ctttgatgca aacaatcaat gcgccagcca
     1681 ttgaagcaaa cgttaatgca atcgcagctg gacatagcgc accgtttatc gtgaacaaac
     1741 ctttctttga cagctttgtc aacttaggag gaacaggtgc aactttaggt ttactgttag
     1801 ctatttattt agtaggacgt aaaaacaaac catatatggt tgtaacaaac ttgtcgattg
     1861 cgccaggtgt cttcaatatt aatgaaccaa cgatgtttgg gttacctatc gtgttgaacc
     1921 cgatcatgtt cattccattt attttgacac caatggtctt a
//
LOCUS       DACECR010000191         1936 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.198, whole genome shotgun sequence.
ACCESSION   DACECR010000191 DACECR010000000
VERSION     DACECR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.198"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..245)
                     /locus_tag="IWO17_002838"
     CDS             complement(<1..245)
                     /locus_tag="IWO17_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0291673.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQ"
     gene            483..1652
                     /locus_tag="IWO17_002839"
     CDS             483..1652
                     /locus_tag="IWO17_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0291674.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          689 a          323 c          300 g          624 t
ORIGIN      
        1 tcttgccata cctcctgaat ttgaccatca ggagtcgtaa tgaaaatagt atcattttca
       61 tctactaatt ctcttaagtt taaatattgt ttactagatg taaaattcga agctgctaat
      121 gacgagtctt cgcttttact ataaaatcct gttacttgta ttttatgttc ctggaaatac
      181 ttcccgaatg aacaacctac cttgccggct ccaataaaac caacgttcat aattatcacc
      241 tccataaaga agtgagacta caagtcccat tcttacgata tgagcttaaa aataaaaatt
      301 aaatcaaata tacagtatgt ttttttcaaa tatcatccat agttaattta tcataatatc
      361 ccatatttta aaataaaaga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
      421 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      481 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      541 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      601 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      661 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      721 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgattcat
      781 tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa atctccaaat
      841 taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac atgagtaaat
      901 tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat tattccgtta
      961 tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag gaagcagtgt
     1021 atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt actattgccc
     1081 caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa cgtgggttag
     1141 aagaagtttt attagtagtg acagacggat taagctgtat tgaagaaagt atccatagtg
     1201 tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt atctagaaat atcgctcata
     1261 aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt caaattggtt taccaagcga
     1321 attcaaaaga agaggcattg gatcacatcg actttatgac taggaaatgg aaaaagcagt
     1381 atccaagagt cgtcaattta ctcttgaatc ctgccctatt aaccttttat aatttccctc
     1441 acgccatcag acgaacaatt tattcgacga acctgattga aggctttaat aagcagctaa
     1501 aacgatatac tcgaagaaaa gaacaattcc ctaatgaaga atctctagag agattcttca
     1561 tttctcaatt taatcaatat aaccaaaaat ttttaggtag aattcacaag ggatttaaag
     1621 aaattcagga tacattagag tcgatgattt aactgtaatt aacagaatgg attttccatt
     1681 tacatataat tcttgacgct accataatct aaataaccat catattcatc tactacttat
     1741 ataattgagt tttattaaac tttttaagtt tctattctat caaatattta aacaatctct
     1801 gcatccgaaa agactacctc gtatagtttt actaacaata tttttattag cgtgttcaaa
     1861 tcctgatttc ttgtatcaat tattttctgt tcactttcaa taagaaaaaa gtctttactt
     1921 ccatggataa taataa
//
LOCUS       DACECR010000192         1894 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.199, whole genome shotgun sequence.
ACCESSION   DACECR010000192 DACECR010000000
VERSION     DACECR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.199"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..273)
                     /locus_tag="IWO17_002840"
     CDS             complement(<1..273)
                     /locus_tag="IWO17_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0291675.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPD"
     gene            456..1307
                     /locus_tag="IWO17_002841"
     CDS             456..1307
                     /locus_tag="IWO17_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291676.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            1328..1540
                     /locus_tag="IWO17_002842"
     CDS             1328..1540
                     /locus_tag="IWO17_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291677.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT          663 a          264 c          378 g          589 t
ORIGIN      
        1 atcgggtgtc catccttgaa caacctttct ttgaatatat tctgattgtt cctcaggtaa
       61 aacaagcggg cgtctaccac agtttgattt gtttttctta tactgttgat agtactctaa
      121 ggctgatttt ccttgcttga agaacaggta tactttatgg atcgtttgtc ttgaacgatt
      181 caagaaatgc gcagttttcg caacagattg atttattttg aaataagact ctatcattac
      241 aagttcatcc gttgtaagat gggtataggt catttgtact cactccttat aattctttgg
      301 acggaactat tttgagtgta gcacaaatga cttttttagt tgtctagctt aattttacaa
      361 tcggcgtact taaaaaattg gttcattgag caacctatcg ctgctaaagt agcaaaggaa
      421 aacgttaaag gaaagaattt agggaagaaa tcgaaatgaa atcggacgac tggattgaac
      481 acacctggct tcctctgaaa agaaatagca aacgaaagaa attttgggta ttgcaattat
      541 ggccattagg aaaactttgt agttacgggg cacgacttat tcttgattta accgtttact
      601 cgattggagt atttggaacg gcttattact tgataaatga tcccaatagg attgcagcat
      661 cagacatttt tacagtgcta atgacgttga tttttgtcat gctattgtct atttttactc
      721 aattagtcat gcaatttacc aggcaaaaat taacagcagg aaaacttttc aaaagcctat
      781 tcgatttaat tgttgtaaat gcaattctag cgactttaat aaaacaaatt gaacaatctg
      841 gaagaacaac aaaaggagtg aaggattttt tattgacgtt tattcacttt gtaaatggac
      901 acatgtattt gtttgaagtg ggtattattc tcttttttgt cgggtatgta tccacgaaac
      961 ttattaaacg ttcaattgaa aaagaaattg aaattgacct gattccgaca cttcttggtt
     1021 atgagtacaa tgttgatttt ggaagatatg acgaattgat caatacggat atcgttcaca
     1081 cgagtatcca tgaatctact gttaagggca tcagatctat tttaaaaaat ggccaggaaa
     1141 tgaaagcaat agatcgaaga aaagagatct acaattttta tgaagtcgaa gcaagcctac
     1201 gatatggcat cggaaaattg tggttagatg gaagtaatat tcgtcaaaac aaaccataca
     1261 ttttttggaa cagtagtgat ttagaacttc caatgaaaga taaataatta tggagaatcg
     1321 tttgaaaatg attcctctat acaggattgt gcttatagtt ggcatggagt taaaaacctt
     1381 aaagaaggag cagttattca aagtgaaaga acgtcataat cagttactat gtagtaaaac
     1441 gggtacgatg aacattgaat accgaacaga atatttagaa ttaacgaatc aaactgattt
     1501 tttgttgatt cgaaagcaac aagagcaaga aaaaaattaa tggctactta tagccaattg
     1561 attgatgaat gggaacaatt caaggaatct aaaattccag aacgtctcta aaagccttta
     1621 tttgtaaaga gcataaggaa acgtgggaac actttttaaa aaaagtgttc caataggctg
     1681 tgcctattac gtttctcaaa aataaatgtg ggaacaaaat atttaaaaat aggatagtga
     1741 aaggatagtg aaggatagta aaaggagggt agtaggatag caaaaaggat aggctccttt
     1801 tcgctatcct ttttaaattg gaggaattaa tatggaagta agaataagaa atgtggatga
     1861 gaaaatcgta agagaaattg atcgaagatg tgac
//
LOCUS       DACECR010000193         1877 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.200, whole genome shotgun sequence.
ACCESSION   DACECR010000193 DACECR010000000
VERSION     DACECR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.200"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            153..1313
                     /locus_tag="IWO17_002843"
     CDS             153..1313
                     /locus_tag="IWO17_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0291678.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            1329..1772
                     /locus_tag="IWO17_002844"
     CDS             1329..1772
                     /locus_tag="IWO17_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0291679.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
BASE COUNT          652 a          300 c          375 g          550 t
ORIGIN      
        1 aacaaaaaat atgagtagct atttttttgg gctgtactta ttacttgatg caggacagct
       61 ttttcacaac ctcatcggct ttcattttct atttttttcc tgaacccata taataaaaga
      121 aagaacaaaa aaacttgcaa gggaggacac atgtgaagca gagtaagcaa aaacagtctc
      181 gtcgactttt gtttcgtttg tttttattaa tatgtatcgt cgtagggtta ctgatcgctt
      241 tatatccatt ttatgtggat tctttaaata gttttattga tcaaaaacgt atcgaacaag
      301 tacaaaaacg gaccgcagct gaaattgaag tgcagcgaaa gaaaatggag gagaaaaacc
      361 aacgattaac tgaccaagga ttcaatcctg gtactgatcc tttcaatgaa aagaacagaa
      421 atgaaagtac aacatccagc caattagagg aatggctgat cggaagtatc aatataccta
      481 agatccaatt aaatatttca ctttatgatc ggctcaatgg taggatcctt gaaaatggag
      541 caggcgtctt gcaaggaacc tctttcccgc ttggtggaaa ttcgacgcat tcagtgatct
      601 ctgctcacag cggtttacct aatcgacggt tatttaccga attagatcgt ttagaaaacg
      661 gagacacgtt tattttgact gttttggggg agaaattggc ttatcaagtt aaaaatatac
      721 aagtggtact gccagatgac acgtctgttc taacgatttc agaaggagaa gacttagtta
      781 ccttgcttac atgtacgccg tatatgatca acacgcatcg tttgctagtg acaggacatc
      841 ggattcctta taacgaatcg gtaaaaaaag aagaaggaaa aggaaatcag gaaagaacca
      901 tgcggcaatg gtttattctt gcaggaacga taatagctgt agtcatactg ttagtgttta
      961 tcggacgact aatctatcag tatagattaa gtaaaaaagt gcttgatttt tcatttatta
     1021 ttagtgattc gtcagggaaa ccggtaaatg gtagttcatt cattttgaaa cataagaaaa
     1081 aaacacttac tagaaatggt gtgccgtttt cagtacaaag cgaccatcat gggaaagtca
     1141 agctggatca attgcctggt ggaacttatc gaatcgtttc agctgatgat cctaaagttg
     1201 ctgtttcttt tggtattaga aaagtaaaac aagagaagat atactttttt gaggggcgaa
     1261 aacttgtaaa agagcttcaa aaaaatggtt tttattttaa aatcaatgaa taagtaagga
     1321 gagaaaaaat gatccgcaca acaacaaaat tgtctgccaa agaactggaa tctgttctat
     1381 cgatttggct gcatgctaac ttggattctc atgaattcat tccatcaact tattgggaga
     1441 atcaagtaga atctgttagg aaagctttac cagatgcagt gatttactta gcagaaatag
     1501 atcaagaaat tattggtttt gctggggtga ttgatcagtt tatcgcagga atctttgtga
     1561 aaagggaata ccggcaacaa agaatcggta cgcaactaat gaaaaaaata aaagaaacca
     1621 ataaaacact gactttgaca gtctatcaaa aaaatcaatc agctattcat ttctatcttt
     1681 cacaaggttt tacaattcaa acacagcaaa aagatgaagc agtaaatgag gaggaacttg
     1741 tgatgaaatg gaatcaaaac gctgattttt gatgaacaga ataacgaaat aatgctccca
     1801 gcctaatttt ttgctgggag cattatttca ttttgaagtc actaatttaa ctgtgtgaat
     1861 atgcagttgt tacaatc
//
LOCUS       DACECR010000194         1751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.201, whole genome shotgun sequence.
ACCESSION   DACECR010000194 DACECR010000000
VERSION     DACECR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.201"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            384..1634
                     /locus_tag="IWO17_002845"
     CDS             384..1634
                     /locus_tag="IWO17_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0291680.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNDSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          577 a          335 c          309 g          530 t
ORIGIN      
        1 tttgtctaat agtttttatt caaaatgttt ctagattata aaatttattg aataacacat
       61 tcctaacgga atttgtttcg atattgtgtg atttctcaga gtacaatgga tgtatggaaa
      121 ggatccaacg ggaatttgct atttcctcct gtagcgtttg tctaccctgt ttagtctgtt
      181 acccgaacca ctgttatcac taccaactca ttagctgttt cgataatgga ctagactttc
      241 aaatagtagt ttggaaggca gcttctagct cttttatcac cgtattctat taggcgaggt
      301 tgtggctata cagtgagttt ctgggaaatc ccacagtatc gaagcatttt ccaaaattta
      361 tatcgaaagg aggtccttct tccatgaaat tatttgtcgg tattgacgtt agctctgaaa
      421 aactagatgt ttgtttttta acagacgaca atcaattgtc tatcttatct gaaatctctg
      481 ttgccaatga catcgaaggt gcttcattca tacgagaaac aattcttgaa ttcaatgaca
      541 gctaccactt tgatcaaatt gtgattggca tggagtcaac gtccatgtac agcttccatc
      601 cttccatgtt ttttcatgaa gatgagaaac taaagaagct caacacactt gtaacgattg
      661 aaaatccttt tcgggtgaaa caatttagtc gcatgtttga tgagaataaa actgatcgaa
      721 acgatgcatt aagaatcgct gatttcttgc gtattcaacg attcacaact tcccctatca
      781 aagaagagaa atacatggcc ttacagcgtt taacacgtac tcgctatcaa cttattaagc
      841 aattgattcg tacaaagcaa cattttcttg aaaatctaac gtacaaatgt aatacgcttg
      901 ctcgagaaat gcgagacgaa tcgacgagtc tttttagtgc aaccctcatt tcactcatga
      961 cggaagattt cacattggat gaacttgctg agcttccttt agaagctttc tgtgatttac
     1021 ttcaagaaaa aggaaaaggc cgtttcaagc aaccagaaaa aatcgctaaa gcaattcaac
     1081 gtgcaattac catgagttac cgcctaggtg ctcttgcaca agaatcaatt aatgttgttt
     1141 taagtgttct cgtacgagaa atacgagcac ttgaaaaaaa tatcaaagaa ttagataaag
     1201 ccatcgaaca agtaattgtt gtcattccag aatatcaatg cttaaccagt attccaggcg
     1261 ttggtaaagt ctatgctgcc ggtttaatcg ctgaaattgg tcaaatcgaa agatttgaag
     1321 atcagacaaa attggcaaaa tacgctggat taagttggaa agtgaaccaa tctggaaact
     1381 atcagtcgca aaatacacct ctgacaaaac aaggaaatcg atattttaga tactacttag
     1441 ttgaagctgc caactctgta aaaaattatc ttcctgaata caaagctttt tatcaaagta
     1501 aatataagga ggttccaaag catcaacaca aacgtgcact cgtcctaacc gcaagaaaat
     1561 ttacgcgtct ggtggatacg ctactacgta aacaccaact ctatacgcca ccaaggagtg
     1621 tgatagagaa ataacaatcc gttattgacg caaatccttg aatcaacccg aaaaaaattt
     1681 gattttgatc gggtctagtt tcgtgcgctt caaaacatat tactcaataa agaaaatctt
     1741 aaatttcatc t
//
LOCUS       DACECR010000195         1736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.202, whole genome shotgun sequence.
ACCESSION   DACECR010000195 DACECR010000000
VERSION     DACECR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.202"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(192..1484)
                     /locus_tag="IWO17_002846"
     CDS             complement(192..1484)
                     /locus_tag="IWO17_002846"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ0291681.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          498 a          366 c          303 g          569 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagagac agcctatcgg caataaggct aaacgtctct ttcactacct cttcaagtta
      121 ttattatcgt atagataaac acttcgactc gctctggtaa gaacgtcctg attcagtcgt
      181 cgtttttatc cttagatttg cgcccagacg ctttctaaga tgtttgtttg tgttctatca
      241 ggtccgacag aaaatgttga gatacgtaca ccaacaagct ctgagatacg acgtacatag
      301 ttacgtgcgt tttctggcag ttctgcaagt gttttgcatc ctgtaatgtc ttctgaccag
      361 cctggcaatt cttcatagat tggtttgcag cggttcaact ctttcaagct tgctggatag
      421 tggtagatta gttcgccatc taattcataa gccgtacaaa tttttactgt ttccaatcca
      481 cttaatacat cgatcgagtt taaagatagg ttggtaatac ctgaaactcg tttagaatgg
      541 cgcataacta cagaatcaaa ccagcctaca cgacgcggac gaccagttgt cgtaccgtat
      601 tctcgtccta cttcgcggat ttgttctcct gtttcatcaa ataattcagt tggaaatggt
      661 ccatcaccga cacgtgacgt ataggctttg cagacgccga caactttatt gatttttgaa
      721 ggccctacac cgcttccgat tgttacccca ccggctactg ggttagaaga agtaacaaaa
      781 ggataagttc cctggtcgat atccagcatt acaccttgtg cgccttcaaa caacacgcgt
      841 ttgcctgcat ccaaagcatc attcaagata acggaagtat ctgtgacgta ttgtttgatt
      901 tgttgtccgt attcataata ttcttcaaaa atatcgtcaa aatcaagtgg ttctgaatca
      961 aacattttta caaattgacg gtttttgtca tctagattca aacgaagacg ttcttcaaaa
     1021 atttctttat ctaataagtc tgcaatacgg atacctacac gagctgcttt atccatatag
     1081 gcaggtccga ttcctttaat cgttgtacca atcttatttt cgcccttagc atcttcttgt
     1141 aattggtcca atttgatatg ataaggcaag attacgtgcg cgcggtcaga aatacgcaaa
     1201 ttacttgttt ctacaccttt ttcatgtaaa taagctaatt ctttaaccaa tgatttagga
     1261 ttgactacaa cgccgtttcc gatcacactg atcttgtctt tataaaaaat cccagaagga
     1321 atcaaatgca gtttataagt cacaccgtta aacttaattg tgtgccctgc attgtcgcca
     1381 ccttggtacc gtgcaatcac ttcagcattt tcactgagaa agtctgtgat ttttcctttc
     1441 ccttcatcgc cccattgcgt acctactact actacagatg acatgtgaac acctcgttct
     1501 atattattta atcctgaacc attgtatcaa taatagatac atatttcaaa taaaatcgaa
     1561 tgatttttat ttttatttta taaaaacgct taaaaaacga actttatctt tttagaatgg
     1621 ttattaaaaa agaggttgta acagaagtgg tcagcttcaa gaaataagaa ggaatgtacg
     1681 aaaattgctt ctcaaatttt tgtgaaattt cgacttattt ttcgaagaag ctactt
//
LOCUS       DACECR010000196         1730 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.203, whole genome shotgun sequence.
ACCESSION   DACECR010000196 DACECR010000000
VERSION     DACECR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.203"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            146..1630
                     /gene="guaB"
                     /locus_tag="IWO17_002847"
     CDS             146..1630
                     /gene="guaB"
                     /locus_tag="IWO17_002847"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ0291682.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          600 a          303 c          399 g          428 t
ORIGIN      
        1 acaatctctt ttaatacaca cagatgtaaa cgctttatat tggtattcta tgagaaagct
       61 aacttgattc caagtagatc attgacttaa atgaaaaaat caattatttt aatagataaa
      121 ctaaattaga ggagtggtac taaaaatgtc caactgggaa acaaagttca cgaaaaaagg
      181 atttactttt gatgatgtct tattgatacc agcagaaagt cacgtgttac caaacgaagt
      241 ggatatgagc gtacaattag ctaaaaatat taagttgaat attccaatca tcagtgcaag
      301 tatggatact gttaccgaca gcaagatggc cattgctatg gcaagacaag gcggactagg
      361 tgtgatccat aaaaacatga cgatcagcca gcaagcggat gaagtaagaa aagtcaaacg
      421 ttctgaaagt ggcgtaatca ttgatccgtt cttcttaacg ccacagcatc ttgtagcaga
      481 cgcagaagaa ttgatgagca aatatcgtat cagtggtgtg ccaatcgttg aaacacttga
      541 aaacagaaaa ttggtaggga taattacaaa ccgcgacatg cgttttgtaa cagattatca
      601 catgccgatt gctgatgtga tgacaaaaga caacttagtg actgccccag taggtacctc
      661 tctaaaagat gcagaaaaga ttttgcaaaa acataaaatc gaaaaattac caatcgtaga
      721 taacgaaggt cgtttaagcg gattgatcac gatcaaagac atcgagaaag tgatcgaatt
      781 tccaaatgca gcaaaagatg aacatggtcg attgcttgta gcagcagccg ttggtgtaac
      841 aagcgataca tttgagcgtg cgaatgcttt attagaagca ggggcagatg caatcatcat
      901 cgatacagct catggacata gtgcaggtgt gatccgaaaa attcaagaaa tccgttcaac
      961 ttttgcagat gcaacattga ttgcaggaaa cgttgcgacg gctgaagcga caaaagcttt
     1021 atatgatgca ggtgtagatg tcgtaaaagt tggtatcgga ccaggatcga tttgtacgac
     1081 acgtgtggta gccggtgtag gtgtacctca gctcacagct atttatgatg cagcatcagt
     1141 tgctcgtcaa tatggcaaag cgattattgc agacggaggg atcaaatatt ctggtgacat
     1201 cgtcaaagca ttagctgccg gcggacatgc agtgatgcta ggaagtatgt tggcagggac
     1261 agatgaatca ccaggtgaat tcgaaatctt ccaaggacgt cgattcaaaa cataccgagg
     1321 tatgggctca ttaggcgcga tggaaaaagg ttcaaaagac cgttacttcc aaggtagtgt
     1381 caatgaagca aataaattag taccagaagg aatcgaaggt cgggttgctt ataaaggaag
     1441 tgttgcagat attattttcc aaatgatcgg cggtttgcgt tcaggaatgg gttatgttgg
     1501 ggcagccaat ctacagcaat tacgtgatga agcgcaattt atccaaatga gcggcagtgg
     1561 attaaaagaa tcacaccctc atgatgtaca aatcacaaaa gaagcaccaa actattcagt
     1621 tgaatcttaa aaacattaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct
     1681 ctaaacacgt ataaacggcg gaagtagaag caactccttc ggaaataaga
//
LOCUS       DACECR010000197         1699 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.204, whole genome shotgun sequence.
ACCESSION   DACECR010000197 DACECR010000000
VERSION     DACECR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.204"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            84..1292
                     /locus_tag="IWO17_002848"
     CDS             84..1292
                     /locus_tag="IWO17_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291683.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
BASE COUNT          645 a          210 c          277 g          567 t
ORIGIN      
        1 aattgaaaat taacgttaaa tcaaaaaaat atttacaaga tgtttattta agtagataat
       61 aaaagaaaaa agcaggtgaa attgtgacag cagaaattgg tattatgaat aaaagtggaa
      121 tcgtgttagc ttcagattca gcatctacga ttggagatag caaagtatat aatactgcaa
      181 aaaaattatt tactttggat tccatgcatt ctgtaggtat catgatatat ggtaatgctg
      241 aatttaatgg tattccttgg gaaataatca ttactcaata taagaaaagc attggtagtt
      301 ctgtttttaa tactttggaa gaatacgcgg ataattttat agaatttgtc aaaacagcat
      361 cttttattag aagtgaacag accgagcaag aacaaatgat tggggttttt caaaaaataa
      421 tttcaggttt atttgagagt atagaagtag atattaactt tttaataagc caaggtacac
      481 aaatagacaa agatgtccta gtgaagttat tacaaacaaa aatgaatacc aatttgtctc
      541 aacagtccca gacattcatt ttagatattg aaaaggcgtt atttcttagt aactatggag
      601 aaatattaaa agatattctt aatagtattt ctacaatgga aggtgtttca gaagctatat
      661 cagaggaaat tcaaagttac gtttatgaaa ttataattag agacgatgtt tattcttccc
      721 ctacaggaat agttatagca ggatatggaa gaatggatat tttccctaaa ctatattctt
      781 ataatatgtt tggatttgtt atgaatattc tgaaatactc agaatatgaa agtgctcaaa
      841 taggaaatga caatggttca ttaagatcta ctatattacc atttgctcag tctgatgtgg
      901 taaatactgt agttcaaggg gtagatccac aaattactaa ttatctgtcg agtcaagtcg
      961 atagttttga cgataacgga aaaaatacct atattaatat tataaagaat atttcagagt
     1021 ttcaacaaaa tcaattcata tttccgttac taaacatgat tgctttactc ccagttgaag
     1081 aaacagcaat tatcgcagag actttattaa atttaaccag ttttaaacga aaatatacta
     1141 cttctgtaga aacagtaggt ggcccaattg atgttttagc tattacacct aatgatggcc
     1201 ctatttggat taaaagaaaa cattattttg atattgataa taatattgga tacagattgc
     1261 gaaaggagag agcgaatgat tacaataatt aaacctcagg aaaaatcccc tgttttgaaa
     1321 tttaatgaga gcaatctagc ccataataaa atgagtataa ctgatacagt atttgatgta
     1381 atggttgagc aacaaaaaaa aattaataat aacaatagac aaagaaaaga aattaaccaa
     1441 tattaatgtt ttttctgttg cgttttttgc agtcataatt taaaacctcg ctcaatcttg
     1501 ggcgaggtct ttttttattt ttctgaaaag ttaaagtaaa taatagcata aaataatata
     1561 ttttacaaag aataagtaca atctagtttt ttgctattaa atgtgtaata ataaatatgc
     1621 catcacaaca tgaagaatga aatccattat tatctagtct agtccattct ttttgtttgc
     1681 agtatttgtg atggctttc
//
LOCUS       DACECR010000198         1664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.205, whole genome shotgun sequence.
ACCESSION   DACECR010000198 DACECR010000000
VERSION     DACECR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.205"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IWO17_002849"
     CDS             131..505
                     /locus_tag="IWO17_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0291684.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IWO17_002850"
     CDS             complement(619..1248)
                     /locus_tag="IWO17_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0291685.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IWO17_002851"
     tRNA            complement(1382..1454)
                     /locus_tag="IWO17_002851"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DACECR010000199         1559 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.206, whole genome shotgun sequence.
ACCESSION   DACECR010000199 DACECR010000000
VERSION     DACECR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.206"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..451
                     /locus_tag="IWO17_002852"
     CDS             <1..451
                     /locus_tag="IWO17_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HAQ0291686.1"
                     /translation="NLVTAARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSG
                     LVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVF
                     KGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            696..>1559
                     /locus_tag="IWO17_002853"
     CDS             696..>1559
                     /locus_tag="IWO17_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0291687.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKLNL"
BASE COUNT          614 a          184 c          316 g          445 t
ORIGIN      
        1 aaacttagtg actgctgcga gagataacga taggttaaaa cagcatgtta gaaatctcat
       61 gagtactgat atggcgagag aatataaaaa attaagtaaa gaacatgggc aagttaaaga
      121 aaaatatagt ggtcttgtag agcgatttaa tgaaaatgta aatgattata atgagttgct
      181 tgaagaaaac aagtctttaa agtctaaaat aagcgattta aagcgtgatg tgagtttaat
      241 ctatgaaagc actaaggaat tccttaagga acgtacagac ggcttaaaag cctttaaaaa
      301 cgtttttaag gggtttgtag acaaggtaaa ggataaaaca gcacaattcc aagaaaaaca
      361 cgatttagaa cctaaaaaga acgaatttga actaactcat aaccgagagg taaaaaaaga
      421 acgaagtcga gatcagggaa tgagtttata aaataaaaaa agcacctgaa aaggtgtctt
      481 tttttgatgg ttttgaactt gttctttctt atcttgatac atatagaaat aacgtcattt
      541 ttattttagt tgctgaaagg tgcgttgaag tgttggtatg tatgtgtttt aaagtattga
      601 aaacccttaa aattggttgc acagaaaaac cccatctgtt aaagttataa gtgaccaaac
      661 aaataactaa atagatgggg gtttctttta atattatgtg tcctaatagt agcatttatt
      721 cagatgaaaa atcaagggtt ttagtggaca agacaaaaag tggaaaagtg agaccatgga
      781 gagaaaagaa aatcgctaat gttgattact ttgaacttct gcatattctt gaatttaaaa
      841 aggctgaaag agtaaaagat tgtgctgaaa tattagagta taaacaaaat cgtgaaacag
      901 gcgaaagaaa gttgtatcga gtgtggtttt gtaaatccag gctttgtcca atgtgcaact
      961 ggaggagagc aatgaaacat ggcattcagt cacaaaaggt tgttgctgaa gttattaaac
     1021 aaaagccaac agttcgttgg ttgtttctca cattaacagt taaaaatgtt tatgatggcg
     1081 aagaattaaa taagagtttg tcagatatgg ctcaaggatt tcgccgaatg atgcaatata
     1141 aaaaaattaa taaaaatctt gttggtttta tgcgtgcaac ggaagtgaca ataaataata
     1201 aagataattc ttataatcag cacatgcatg tattggtatg tgtggaacca acttatttta
     1261 agaatacaga aaactacgtg aatcaaaaac aatggattca attttggaaa aaggcaatga
     1321 aattagacta tgatccaaat gtaaaagttc aaatgattcg accgaaaaat aaatataaat
     1381 cggatataca atcggcaatt gacgaaactg caaaatatcc tgtaaaggat acggatttta
     1441 tgaccgatga tgaagaaaag aatttgaaac gtttgtctga tttggaggaa ggtttacacc
     1501 gtaaaaggtt aatctcctat ggtggtttgt taaaagaaat acataaaaaa ttaaacctt
//
LOCUS       DACECR010000200         1472 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.209, whole genome shotgun sequence.
ACCESSION   DACECR010000200 DACECR010000000
VERSION     DACECR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.209"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>1472
                     /locus_tag="IWO17_002854"
     CDS             <1..>1472
                     /locus_tag="IWO17_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0291688.1"
                     /translation="RDRRFDVTLLINGLPIIQIELKQVTAKDGFYQAFNQIKKYAEEG
                     MFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKFLFSWRTKDNKKVENLYEFCKQV
                     LNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAIQALFIAANKHQSGYVWHATGSG
                     KTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTTEFTKFASEFNTGVSSGNAKANS
                     LIVGTGSAKELSETLLADANANVVIITTRQKLDAALKYAKKQEEKKGTNRFQKLMGQH
                     IVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQYG
                     EVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSPEQINRM
                     IDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTTSS
                     IDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDPD"
BASE COUNT          496 a          264 c          320 g          392 t
ORIGIN      
        1 cgggatcgac gttttgacgt caccttattg atcaatggct taccgattat ccaaatcgag
       61 ctaaaacaag taactgcaaa agatggtttt tatcaggcgt ttaatcaaat caaaaaatat
      121 gctgaagaag gcatgtttcg gaacaatatc ttttcgacct tacagttgtt tgtggtttcc
      181 aatgaacaaa cgactcggta ttttgcaaat gccttaccga aggatttgca tcctaagttt
      241 ttatttagtt ggcgaacgaa agataataaa aaggtagaaa acttatatga gttttgcaaa
      301 caagtgctaa atatcccaga tgcccatcgc ttgattgcgg attatacgat tgtcagtgaa
      361 gatcaagaca ataaaaccct catggtgtta catccttatc aagtgcatgc gattcaagcc
      421 ttgtttattg ccgcaaataa acatcaatca gggtatgtgt ggcatgcgac tggttcagga
      481 aagacgttga ctagttttgt atctactaag cttttagcac gaaaatcggg gatcgatcga
      541 acgatcatgt tagtggaccg caaagattta gataatcaga ccaccacaga gtttacgaaa
      601 tttgcttcgg agtttaatac tggggtttct tctggtaatg ccaaagcgaa tagcttgatc
      661 gtgggaacag gaagtgccaa agagttaagt gaaactctgt tggcagatgc caatgcgaat
      721 gttgtgatca ttacaacccg tcaaaaacta gatgcggcgt tgaaatatgc aaagaaacag
      781 gaagagaaaa aaggaaccaa tcgcttccaa aaattaatgg ggcaacatat cgtgtttgta
      841 gttgatgagt gtcatcgagc gttaagtgct gaaaatatgg aagaaatcaa aaaaatgttt
      901 ccgaaatcca cttggtttgg ctttacaggt acaccgattt tccctgagaa tcggaaacaa
      961 gccaaagggc aattggcacg tacgacccat gatcagtatg gagaggtctt gcacacctat
     1021 accatcaaga atgcattaga agatggctcg gttttaggat ttcaagtaga gcatgaaaac
     1081 accgtggagc cgacttcatt agagaataaa atctatcgga aattaaaaga agtagaaaca
     1141 tatgcagagt attcaccgga acagatcaat cgtatgatcg atcaaatgga gtctgtcaaa
     1201 aaagaaagtt atttggaccc tactgttttt gaagcagagg aacatatcca aaaagtcatt
     1261 cataaaatgt tccgtccaga caatgcctat acaaaatttg attttcgaaa tggccgtcca
     1321 acgaagtctg cgatcttaac cacgagttcc attgatatgg ccaaaaagta ttatcgggcc
     1381 atcaaagaga tgacgaaaga atctgattgg ttaataaaag agtttcccaa tcagcctatc
     1441 cgtgaaggac gaacgatgga ggatcctgat tt
//
LOCUS       DACECR010000201         1457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.210, whole genome shotgun sequence.
ACCESSION   DACECR010000201 DACECR010000000
VERSION     DACECR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.210"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..119)
                     /locus_tag="IWO17_002855"
     CDS             complement(<1..119)
                     /locus_tag="IWO17_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0291689.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSS"
     gene            complement(130..327)
                     /locus_tag="IWO17_002856"
     CDS             complement(130..327)
                     /locus_tag="IWO17_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0291690.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(343..585)
                     /locus_tag="IWO17_002857"
     CDS             complement(343..585)
                     /locus_tag="IWO17_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0291691.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            complement(620..757)
                     /locus_tag="IWO17_002858"
     CDS             complement(620..757)
                     /locus_tag="IWO17_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0291692.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            complement(759..1205)
                     /locus_tag="IWO17_002859"
     CDS             complement(759..1205)
                     /locus_tag="IWO17_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291693.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
BASE COUNT          385 a          319 c          230 g          523 t
ORIGIN      
        1 gagctacctt catttacacc taaattaaat tcattgttga tcgtataggc gaacccgtta
       61 attggcaata aaaaaagagc cattattagg ctcaatgata aaatgatttt cttcttcatt
      121 tttttcctcc tattttttta agttatatgc agacacacca gtgataacgc ctaaaaatgt
      181 tgctactgca ttgatagtga gtactgtcat atctgttcca ttccatccat acgcttttcc
      241 taacgtggct actaaaacag atgcagctgg taatactgtt aaaaccgtcc atttaatgac
      301 ttgataatac ttatcgggta aaatcatttc ttcttaactc ctttacaatt tagtcaagaa
      361 atagccaatg atcgtaatgc ctaaaccgat catgtaaccc cacgaccatt tattattggc
      421 tttcatttct ttgatatctt ctgcgttgtt tagcgctact gagtaggctt tgtctgccaa
      481 atcttttgca ctatcagctt tttctctaag tgattcgtag ttgtctaatt ttgtttcaat
      541 tcttactaat ctctccacaa cgtcttggag cgcttcttct ttcatgttcc accaactttc
      601 caacaaaaaa acgcatcact taagatgcgc tctcttcttt gctaataatt ctatctgctt
      661 cttcatctgt gatacatagt ggaacgaata gacgaacttg atcgtcagta aaacagcccc
      721 aatcatacat cattttcaca tcgctaaaac taaacatact actcacctcc ctttgaagct
      781 ggatttagtt gctctttaat ttctgaaatg tctttgctat tttgtaacga agcaagcatc
      841 atttttgaat tgatttgtgc taaactgtca gctttttctt tcaatgcagt attttcctgt
      901 ttaatcgcta catcgcttag catgagtttg gcatttagct gttttaggtt gccgttttca
      961 tgttcaagag cctcgtacat cgctttgaga ttgtttaaat cgttgtgatc taacgcgttc
     1021 gctaaaataa tccattggtt caatttagga tcaaacatct gatcagcaat cgttagcggt
     1081 tcgccatctg aacgaacccc ttcgagcggt ggctgatctg tgtaaggaac ggatacaagc
     1141 atgtcgtcca atacttttcc tgcatactct ccgccagtac ggccatattt ccaaatgttt
     1201 ttcatttatt tccctcccag taattgaatt tcggtttcca atttggaatc ggtggttcga
     1261 cttctgtgca ttcttccggt aaatgttctt catcattcac aatgatttgc tcgaatccgt
     1321 aaggttcaat tggtctatat gctgccttca tatcgattca cgcctttcta aatcgtgtaa
     1381 gtaattgcaa aggtataatc cgatccataa cttgagtttc ttcgccattt aatggctcca
     1441 tctgcaccaa tagataa
//
LOCUS       DACECR010000202         1456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.211, whole genome shotgun sequence.
ACCESSION   DACECR010000202 DACECR010000000
VERSION     DACECR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.211"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            108..1040
                     /locus_tag="IWO17_002860"
     CDS             108..1040
                     /locus_tag="IWO17_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ0291694.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="IWO17_002861"
     CDS             1112..>1456
                     /locus_tag="IWO17_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291695.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DACECR010000203         1455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.212, whole genome shotgun sequence.
ACCESSION   DACECR010000203 DACECR010000000
VERSION     DACECR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.212"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            5..1222
                     /locus_tag="IWO17_002862"
     CDS             5..1222
                     /locus_tag="IWO17_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0291696.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            1235..>1455
                     /locus_tag="IWO17_002863"
     CDS             1235..>1455
                     /locus_tag="IWO17_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0291697.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLR"
BASE COUNT          519 a          228 c          294 g          414 t
ORIGIN      
        1 aaacatgagc aatgatacac aaccagaaat ccgtttccca ggatttacag atgattggga
       61 acagaggaag ttgggggact tcgttgtaga ttatgttgaa aagacaagtg tacaaaatca
      121 atttcctatg ctaacttcat ctcaacaaaa agggatagtt ttgcaagaag actattttgc
      181 aaatcggcaa gtaactacag aaaataatat tggttacttt gtactaccta gaggatattt
      241 cacgttccgt agtcgaagtg ataatgatgt gtttgtgttt aaccgtaatg acattattga
      301 tagagggatt ataagctatt tttatcctgt atttacttta aaatctgctg attccgactt
      361 cttcttaaga cgtattaaca atggaatcca acgacaatta tctattcaag cagagggaac
      421 aggtcaacat gttctgtctc taaaaaagtt taaaaatata gtagcaatgt ttcctagtga
      481 ggaagaacaa caaaaaatcg gcaccttctt caaacaactt gacgacacga tcgctcttca
      541 tcagcgtaag ttagatttgc tgaaggaaac gaaaaaagga ttcttacaaa aaatgttccc
      601 tgaaaacgga gcaaaagtgc cagaaatccg gtttcctggt tttacggaag attgggaaga
      661 gcgtaagttg ggagaattag gtaaaactca atcgggaatt ggatttcctg atgccgagca
      721 aggtggttct gaaggtactc cattttttaa agtttccgac atgaataaca ttggtaatga
      781 gtatgaaatg agaaatgcaa atcattatgt atctaatgag caaattgagc gtaaaaaatg
      841 gaagccaata aaagatgtgc cagcgattat atttgctaaa gttggcgcag caataatgct
      901 taatagaaaa cgtttggtca caagcccttt cttaatagat aacaatacca tggcttattt
      961 atttaataat acttgggata tctattttgg taaaatattg tttgaaacta taaatttgcc
     1021 aagatactcc caagtagggg cattgccttc ttataacagt tctgatattg agaatatcag
     1081 tgttaaggtt cctgtaaagg atgaacaaca aaaaatcggt actttcttca aacagctaga
     1141 tgatacgatc gctcttcatc aacgtaagtt agatttacta aaagaaacga aaaaaagctt
     1201 cttacaaaag atgtttgtct aggagggaag aagaatgaaa attagtcacc gtgaggaagc
     1261 agaagtcgaa gaacagttga ttcgtgtact tggagaaggg cataatcagt ggacgtatcg
     1321 tccggatcta aaatcagaag aagacctttg ggtcaattta cgccaaaaaa ttatctcaaa
     1381 taatcaagca gaactgaatg attttccttt gaccgataaa gaatttgaaa cgatcaaaac
     1441 ggaattactg ctgcg
//
LOCUS       DACECR010000204         1441 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.213, whole genome shotgun sequence.
ACCESSION   DACECR010000204 DACECR010000000
VERSION     DACECR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.213"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            226..1101
                     /locus_tag="IWO17_002864"
                     /note="aph(2'')-Ia"
     CDS             226..1101
                     /locus_tag="IWO17_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ0291698.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
     gene            complement(1231..>1441)
                     /locus_tag="IWO17_002865"
     CDS             complement(1231..>1441)
                     /locus_tag="IWO17_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0291699.1"
                     /translation="FQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANR
                     LIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          585 a          128 c          243 g          485 t
ORIGIN      
        1 acaagatata ttaaattgat ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt
       61 attttagacc ctcataaaaa taatccaaga gcaataaggg cataccaaaa atctggtttt
      121 agaattattg aagatttgcc agaacatgaa ttacacgagg gcaaaaaaga agattgttat
      181 ttaatggaat atagatatga tgataatgcc acaaatgtta aggcaatgaa atatttaatt
      241 gagcattact ttgataattt caaagtagat agtattgaaa taatcggtag tggttatgat
      301 agtgtggcat atttagttaa taatgaatac atttttaaaa caaaatttag tactaataag
      361 aaaaaaggtt atgcaaaaga aaaagcaata tataattttt taaatacaaa tttagaaact
      421 aatgtaaaaa ttcctaatat tgaatattcg tatattagtg atgaattatc tatactaggt
      481 tataaagaaa ttaaaggaac ttttttaaca ccagaaattt attctactat gtcagaagaa
      541 gaacaaaatt tgttaaaacg agatattgcc agttttttaa gacaaatgca cggtttagat
      601 tatacagata ttagtgaatg tactattgat aataaacaaa atgtattaga agagtatata
      661 ttgttgcgtg aaactattta taatgattta actgatatag aaaaagatta tatagaaagt
      721 tttatggaaa gactaaatgc aacaacagtt tttgagggta aaaagtgttt atgccataat
      781 gattttagtt gtaatcatct attgttagat ggcaataata gattaactgg aataattgat
      841 tttggagatt ctggaattat agatgaatat tgtgatttta tatacttact tgaagatagt
      901 gaagaagaaa taggaacaaa ttttggagaa gatatattaa gaatgtatgg aaatatagat
      961 attgagaaag caaaagaata tcaagatata gttgaagaat attatcctat tgaaactatt
     1021 gtttatggaa ttaaaaatat taaacaggaa tttatcgaaa atggtagaaa agaaatttat
     1081 aaaaggactt ataaagattg attatataat atatgaaaag ctattataaa agacattagt
     1141 attaaatagt ttaaaaaaat gaaaaataat aaaggaagtg agtcaattcc agactcctgt
     1201 gtaaaatgct atacaatgtt tttaccattt ctacttatca aaattgatgt attttcttga
     1261 agaataaatc cattcatcat gtaggtccat aagaacggct ccaattaagc gattggctga
     1321 tgtttgattg gggaagatgc gaataatctt ttctcttctg cgtacttctt gattcagtcg
     1381 ttcaattaga ttggtactct ttagtcgatt gtgggaattt ccttgtacgg tatattgaaa
     1441 g
//
LOCUS       DACECR010000205         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.214, whole genome shotgun sequence.
ACCESSION   DACECR010000205 DACECR010000000
VERSION     DACECR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.214"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IWO17_002866"
     CDS             complement(150..1337)
                     /locus_tag="IWO17_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ0291700.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACECR010000206         1415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.215, whole genome shotgun sequence.
ACCESSION   DACECR010000206 DACECR010000000
VERSION     DACECR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.215"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..267)
                     /locus_tag="IWO17_002867"
     CDS             complement(<1..267)
                     /locus_tag="IWO17_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291701.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILG"
     gene            392..1177
                     /locus_tag="IWO17_002868"
     CDS             392..1177
                     /locus_tag="IWO17_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAQ0291702.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT          529 a          231 c          216 g          439 t
ORIGIN      
        1 ccccaaaata tcaacgattg tggcaatcag gtcttctttt gattcttcat ctgaaagtaa
       61 ttcacgatac aacattgttt gtttgcttac tgtgtcatat tcctctaagg attcatctgc
      121 taaaagaacc acttgttcca acaaacggtc ggataaatat ttatagaaaa tcagtcccaa
      181 taaataattt ttatattctg acgcatccat tttactacgg agattatctg ccgcactaaa
      241 taatttttga tttaattctg cacccaaaaa gatacactcc tatctcttat ttaaagttgc
      301 tatataccct cttattttag catactccct tttagaaaaa gcaaaaaaaa aagaaaaaat
      361 cttattcttg ataacaagct acgtgacgta tatgcctaac aaaaatagaa cttttgaatc
      421 aatcttaaat gaaggtttaa ccacagaacc ggcatccatt atttttgatg aagttatcga
      481 tgacgttgaa gatagtaagt atcataaaaa tttaaaagaa cttttttatt ttgcactcaa
      541 caatggacca agtttgttca ctggtatatc agtacctgat agtgataatc cattaattat
      601 ggcaaaaatt tacttaacaa aatggtgtaa taaatacatc agggatagga ataacccagc
      661 attaaaaaaa cctcttaaaa catttggcga aaaagatgct gctttaacta ctagagtagc
      721 agcaaatgct aatattgatg atcaaaaagt attgaatgac tacttgcgtg gacatttttt
      781 atatatgagc gctgagaaca tgaacggaag tattttagag gaatatttag ccgaagtgtt
      841 agaacctgaa ggttggattt ggtgcgccgg ctctgtatat cgagctgttg atttctgtta
      901 tttaggaacc tcaccaattc ttttacaagt gaaaaataaa tacaatacag aaagcagttc
      961 gagttcagct ataagagtag gaacaacaat tagaaaatgg aatcgtttaa ataagtcgac
     1021 aaaaatttct ggtttggata gccctatccc caattggaaa gcattaatag aaatgactga
     1081 agctagtaag gaactggcag ctaagttaac tgaaaatagt tatttagctt atattaatga
     1141 aaaatccaca cgagaattat ggacactcga tgactaacaa tatccttatt agtatctatc
     1201 cacgatacat ggaaaaaatt ataaacaaac aaaaaaactt tgaatttaga aatttctatc
     1261 ttacctcaga tccttataat atcttttgga tttacgaaac aatccccacc aaagctttaa
     1321 agtataaatt gattgtaaaa aatccaatta ctaaacttaa gaaaaatcaa cactactttt
     1381 taggagacga tgagtttgaa caattagttt aaaag
//
LOCUS       DACECR010000207         1360 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.216, whole genome shotgun sequence.
ACCESSION   DACECR010000207 DACECR010000000
VERSION     DACECR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.216"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(136..1284)
                     /gene="tnpB"
                     /locus_tag="IWO17_002869"
     CDS             complement(136..1284)
                     /gene="tnpB"
                     /locus_tag="IWO17_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0291703.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          390 a          241 c          216 g          513 t
ORIGIN      
        1 cctgacggtt cttaacgctt gaagtgggag cttcttggga atagagcata cttgatagcg
       61 tatttcttta ctaacagcgg tgtctcttat ttaccaagct atccccgtag ttcctacggt
      121 tcttgatgat tggatctaag ccaatcccat agaatgtagt cttagacctt cggtcagaat
      181 attgatacta gcattgatat cacgatcatg atgagtatga caaacaggac aagtccattc
      241 tcgaatttca agagatttct tgccatcttt atgtccgcac tctgaacaaa tctgactaga
      301 cggaaaccat ttatctactt tgatgatttt tcgtccatac cagtcagcct tgtattgtaa
      361 tttagtcaca aactttgacc aagaaacatc agaaatactt ttagctaatt tatgatttcg
      421 caacatacct tttgtgttta agtcttcaat acagataata tcgtgatttt tgatcatttc
      481 tgtactcaac ttattcagaa aatcagtacg ttgattcatt actttttcat gcaatctagc
      541 tactttgcgt ttttgttttt gataattttt agcttcaaat agattggtac ctttattttt
      601 agccaataaa gcacgcctag acaatttgcg ttgttcacgc tttagtttct tttccatttt
      661 ggacgtgaat ttattattat caattttttg tccatcagaa agaatcgcaa agtccataat
      721 acctaagtca ataccaattg tggaattagt tttaggtaat tcgataattt cttctttaca
      781 caacaaagaa atatagtatt taccgctaga atgacgtgat attgtagcag atttgataat
      841 cccttttggt tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat
      901 gaatttacta tcaatcaaag caattgtacc attttgatta ttcgttgtat aactctgaac
      961 aggatttttc ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt
     1021 gtatgcttta tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa
     1081 aaacggaaat tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc
     1141 atttttaact tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg
     1201 gacacaacca aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa
     1261 tttgtatgct tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt
     1321 gattttggat atacttctta atcacatcaa ttggtgcgcc
//
LOCUS       DACECR010000208         1313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.218, whole genome shotgun sequence.
ACCESSION   DACECR010000208 DACECR010000000
VERSION     DACECR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.218"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..260
                     /locus_tag="IWO17_002870"
     CDS             51..260
                     /locus_tag="IWO17_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291704.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="IWO17_002871"
                     /pseudo
     CDS             467..667
                     /locus_tag="IWO17_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          447 a          215 c          247 g          404 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aat
//
LOCUS       DACECR010000209         1277 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.219, whole genome shotgun sequence.
ACCESSION   DACECR010000209 DACECR010000000
VERSION     DACECR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.219"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            141..398
                     /locus_tag="IWO17_002872"
     CDS             141..398
                     /locus_tag="IWO17_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291705.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IWO17_002873"
     CDS             complement(630..1043)
                     /locus_tag="IWO17_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ0291706.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          487 a          159 c          159 g          472 t
ORIGIN      
        1 ttctgtccac gttcatatta ctccaaagtt ttcctcattc caaaagaaaa gcacagaata
       61 cacaaagaag cgtttttatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatccc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta aaatataagc
     1201 tatttgttta cttctgtaga atatatactt tgttgttaga taagttgcac atgtttatag
     1261 aatctaaata ataaatt
//
LOCUS       DACECR010000210         1274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.220, whole genome shotgun sequence.
ACCESSION   DACECR010000210 DACECR010000000
VERSION     DACECR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.220"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(127..462)
                     /locus_tag="IWO17_002874"
                     /pseudo
     CDS             complement(127..462)
                     /locus_tag="IWO17_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            644..1249
                     /locus_tag="IWO17_002875"
     CDS             644..1249
                     /locus_tag="IWO17_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0291707.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
BASE COUNT          463 a          204 c          204 g          403 t
ORIGIN      
        1 gaaaaattaa aaacacaaga atcgttgatt tattagtatt cttgtgtttt caattttata
       61 cattcaaaaa tagtatctgg aacatccgtg cgaaaatgca cggtaggaac caaaagacga
      121 acattctcaa taattctcta tatttaacgg acgtagtatt ccattcgata attctttttc
      181 atagcggaaa ctatatttgc caacaaaatt aatgtgatca ctaattaatg gaaattgctt
      241 gcgtaacact tcttcggaac agtcgaaccc ttccgtttta atttgttcaa agactttttc
      301 taaatatagt gaattccaat atatgatagc attagttact aatcctaatg cgttgagctg
      361 ttcctccata ccgtcgtaat aagtttgata aagtttgcct tttctaccat aaaatatatt
      421 tcgacataga gcatgacgag cttcaccttt atttaactgt tccgagctag gctctcctaa
      481 ataaacacca aaagaatttt tattgcgctt aatttcattc attcaaaacc tccaaagtaa
      541 attataagta gacttaaaat ttatttttaa aaatgctatg ataacagtaa aactacaagt
      601 aaattttaca tgtaaattat atcatttttt aaaaggagtt tttatggcaa aaattggata
      661 tattagagtg tcttcacaag atcaaaattt agatcgacaa ttggcaatga tggaagacct
      721 taacattgat aaattattcc aagaaaaagc cagtggtaaa gatatccagc gacctgagtt
      781 tcaaaaatta ctaaattata tccgagaagg tgattgtgtc attgttgcat cactggatcg
      841 cctaggcaga gattatgaag acataaaaaa tacagttacc tttatgaaac agaaaaaagt
      901 agcattaaaa atactagatg cacagttttt agattttaat actggaaatg aactcctaga
      961 tactgctatg ttcgatatgt ttttatcatt gctaagttat attgcacaaa acgaacgtga
     1021 gaaaatccgt gaacgtcagc ggcaaggagt attattggcc aaaaaagctg gtcgatataa
     1081 ggggcgacca attgaatatt ctgagaactc aacagatccg caaaaacgat tagtctttaa
     1141 aactgtggtg gacatgttaa atcaaggagt tccagttgca gaaattgcca aagaaaatgg
     1201 tctctctcga ccaaccatat ataaaataaa gaaagcgaat gatttgtaga atttatacta
     1261 tattcattcg cttt
//
LOCUS       DACECR010000211         1256 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.221, whole genome shotgun sequence.
ACCESSION   DACECR010000211 DACECR010000000
VERSION     DACECR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.221"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            82..>1256
                     /locus_tag="IWO17_002876"
     CDS             82..>1256
                     /locus_tag="IWO17_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ0291708.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     "
BASE COUNT          416 a          247 c          241 g          352 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatgg
//
LOCUS       DACECR010000212         1253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.222, whole genome shotgun sequence.
ACCESSION   DACECR010000212 DACECR010000000
VERSION     DACECR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.222"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..234
                     /locus_tag="IWO17_002877"
     CDS             <1..234
                     /locus_tag="IWO17_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ0291709.1"
                     /translation="KLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPKSDGGTKV
                     VMNTKWTQKGRLFIYESLKKEGYIPEIDLLEER"
     gene            237..449
                     /locus_tag="IWO17_002878"
     CDS             237..449
                     /locus_tag="IWO17_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0291710.1"
                     /translation="MSQDFILKVRVALATHNKSQAWLAEKLDISTAYMSDIMNGRRKP
                     DKQIKPIEAVLAELEKEKKHANNNSR"
     gene            427..711
                     /locus_tag="IWO17_002879"
     CDS             427..711
                     /locus_tag="IWO17_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291711.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVRKVLGIGDDKLTHWINLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            732..920
                     /locus_tag="IWO17_002880"
     CDS             732..920
                     /locus_tag="IWO17_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291712.1"
                     /translation="MSYTLQQEHQILRLIKQRRKQLQDDREALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
BASE COUNT          495 a          195 c          255 g          308 t
ORIGIN      
        1 aaactacttc ataaactagg tattcagaaa aaagtaggca accaatggtt attatgcaag
       61 aaacatatga gacaaggata tacaaaatct catacaactg agattccgaa atctgatggt
      121 ggaacaaaag ttgtgatgaa tacaaaatgg actcaaaaag gacgtttgtt catttatgag
      181 tcgctaaaaa aagaaggata tattccagaa attgatctat tagaggagag atagttatgt
      241 cgcaggactt cattttaaaa gtgcgtgtgg cgctagctac gcataacaag agtcaagctt
      301 ggttagctga aaagcttgat atttcaacag cctatatgtc ggatattatg aacgggcgta
      361 gaaaaccaga taaacaaatc aagccaattg aggcagtact agcagaatta gaaaaggaga
      421 aaaagcatgc aaataacaat tccagataat ttagtagttt cagaattaac tacacaaatt
      481 acgaatgctg tgcttaattc attggacgaa cgattgcacc ttatgaacaa atcagtagag
      541 cttcctccat atccaaacaa atcagaggta agaaaagttt taggaattgg tgatgacaaa
      601 ttaacacatt ggataaacct aggcttaaaa acacagcagt ggagcaagtt agacatcaga
      661 attgaacgat cggaactcca aagatttttg aaagaaaact ttgagttcta aaggcaaagg
      721 agaatgattt tatgtcctac acattgcaac aagaacatca aattctccgt ttgattaaac
      781 aacgcaggaa acaattacaa gatgatcgtg aagcgcttag aaaagccgat gagctatcag
      841 atagacaagc tgaactaatc gcttctgaac ttgaggattt gagaatgcta gaaataaaaa
      901 atagggagat tagattatga agaagacaga cacacttttt ataggattca tcctagggct
      961 attagtgata gtagtacacc aaagtattat cgggggaagt ttgttcgcag cattgatggt
     1021 tttaatcaat ctgcttgatt caaaagaaag gagcaactat ggcacgagaa gaagcgctaa
     1081 aaatcggtaa agtgattgct gataattggt gggcaaatag ccgtcctatt attttaagca
     1141 agcaacatat cgaaaagcaa aaagcatggc aacaaataaa aaagtgactc cgccgaccaa
     1201 agcaatgagt cactcaaaaa atcataacta aggagatttt aacatatgga aaa
//
LOCUS       DACECR010000213         1252 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.223, whole genome shotgun sequence.
ACCESSION   DACECR010000213 DACECR010000000
VERSION     DACECR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.223"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            52..>1252
                     /locus_tag="IWO17_002881"
     CDS             52..>1252
                     /locus_tag="IWO17_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0291713.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHI"
BASE COUNT          385 a          250 c          245 g          372 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat ca
//
LOCUS       DACECR010000214         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.224, whole genome shotgun sequence.
ACCESSION   DACECR010000214 DACECR010000000
VERSION     DACECR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.224"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IWO17_002882"
     CDS             200..742
                     /locus_tag="IWO17_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291714.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IWO17_002883"
     CDS             832..>1246
                     /locus_tag="IWO17_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ0291715.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACECR010000215         1227 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.225, whole genome shotgun sequence.
ACCESSION   DACECR010000215 DACECR010000000
VERSION     DACECR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.225"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..779)
                     /locus_tag="IWO17_002884"
     CDS             complement(<1..779)
                     /locus_tag="IWO17_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0291716.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIM"
     gene            complement(790..996)
                     /locus_tag="IWO17_002885"
     CDS             complement(790..996)
                     /locus_tag="IWO17_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0291717.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            complement(1129..>1227)
                     /locus_tag="IWO17_002886"
     CDS             complement(1129..>1227)
                     /locus_tag="IWO17_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0291718.1"
                     /translation="KRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT          342 a          259 c          202 g          424 t
ORIGIN      
        1 tccataatga agatatactc gtatttatag ttagcaatgt gccatccagc tacatgcaaa
       61 tttgcatttt cgatttctcc aaactgatca atgtgggcgt aattcgttcc atctgtcagt
      121 gtaggattag ctgcaccagc tcgtgttgga tcaatgacag gcttgttttc agaagttgtt
      181 ggattttcat cggtaaatcc atgagctaaa tcatatgcta atttttcttt acttacgccc
      241 atttcagaaa gataaccgta aggatctgta tgatcgcccc agatgttttg tgttacccat
      301 aaatgcgatt tgattcccgg ttggttataa ggagtgtcca acgttaatgg aataccatat
      361 ttcattgctg aatctctagc caattcaacg tatgccttgt agtttttctc aaacgttgct
      421 ttatcatgtg tgtgttgtaa ctcaatctgc acaggactgt tggcattagc atacgaacca
      481 gcaccgtact gtacataacc aggttgaccg acttgataaa caattccgcc gtctcccaca
      541 atataagcag tataagcgct agtccatgaa cgttgcatat actgcgcttc attgcgtcct
      601 gttgctgttt cattagccgt ttcatgcagt aaaatatact gattatttgc tacttgtgag
      661 ctaccttcat ttgcgcccaa attaaattca ttgttgatag tataggcaaa tccgttaatt
      721 ggcaataaaa aaagagccgt taacaggctc atcgcagtaa tagtaatttt ctttttcata
      781 tctatcctcc tatttatctg atgaaatttt cttatacgtt cgatttgata cacctaaaat
      841 actccctaga aatgctccaa aacctgtaat aatcatcacc gttatatctg tgtaccgcca
      901 gttaacacct tttccgacaa gaccgacaaa agtcgctaaa gcggggataa ctactatagc
      961 gatccatttc aaaatatcaa aggttctatt gttcatacta tccacctccc ttcattggtt
     1021 gggctacttt ctttttcaat cgcttgtttt cttctatcaa tatttctctt tctgaaagca
     1081 aggcatcaat ttgcccgtcc agttgttcaa ttttatcttt cttaattgtc aaattaagtt
     1141 agacagatca tcaatactta cgtaactcaa aaatacttcc aaaggtgttc gataatccag
     1201 tgattttcta ggaatattat ttcgttt
//
LOCUS       DACECR010000216         1225 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.226, whole genome shotgun sequence.
ACCESSION   DACECR010000216 DACECR010000000
VERSION     DACECR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.226"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..978
                     /locus_tag="IWO17_002887"
     CDS             4..978
                     /locus_tag="IWO17_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0291719.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          418 a          159 c          207 g          441 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact attactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tggactaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcctg
//
LOCUS       DACECR010000217         1214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.227, whole genome shotgun sequence.
ACCESSION   DACECR010000217 DACECR010000000
VERSION     DACECR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.227"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1214)
                     /locus_tag="IWO17_002888"
     rRNA            complement(<1..>1214)
                     /locus_tag="IWO17_002888"
                     /product="16S ribosomal RNA"
BASE COUNT          256 a          372 c          272 g          314 t
ORIGIN      
        1 tgtacttccc attgtagcac gtgtgtagcc caggtcataa ggggcatgat gatttgacgt
       61 catccccacc ttcctccggt ttgtcaccgg cagtcttgct agagtgccca actgaatgat
      121 ggcaactaac aataagggtt gcgctcgttg cgggacttaa cccaacatct cacgacacga
      181 gctgacgaca accatgcacc acctgtcact ttgcccccga aggggaagct ctatctctag
      241 agtggtcaaa ggatgtcaag acctggtaag gttcttcgcg ttgcttcgaa ttaaaccaca
      301 tgctccaccg cttgtgcggg cccccgtcaa ttcctttgag tttcaacctt gcggtcgtac
      361 tccccaggcg gagtgcttaa tgcgttagct gcagcactga agggcggaaa ccctccaaca
      421 cttagcactc atcgtttacg gcgtggacta ccagggtatc taatcctgtt tgctccccac
      481 gctttcgagc ctcagcgtca gttacagacc agagagccgc cttcgccact ggtgttcctc
      541 catatatcta cgcatttcac cgctacacat ggaattccac tctcctcttc tgcactcaag
      601 tctcccagtt tccaatgacc ctccccggtt gagccggggg ctttcacatc agacttaaga
      661 aaccgcctgc gctcgcttta cgcccaataa atccggacaa cgcttgccac ctacgtatta
      721 ccgcggctgc tggcacgtag ttagccgtgg ctttctggtt agataccgtc aagggatgaa
      781 cagttactct catccttgtt cttctctaac aacagagttt tacgatccga aaaccttctt
      841 cactcacgcg gcgttgctcg gtcagacttt cgtccattgc cgaagattcc ctactgctgc
      901 ctcccgtagg agtttgggcc gtgtctcagt cccaatgtgg ccgatcaccc tctcaggtcg
      961 gctatgcatc gtggccttgg tgagccgtta cctcaccaac tagctaatgc accgcgggtc
     1021 catccatcag cgacacccga aagcgccttt caaatcaaaa ccatgcggtt tcgattgtta
     1081 tacggtatta gcacctgttt ccaagtgtta tccccttctg atgggcaggt tacccacgtg
     1141 ttactcaccc gttcgccact cctctttttc cggtggagca agctccggtg gaaaaagaag
     1201 cgttcgactt gcat
//
LOCUS       DACECR010000218         1153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.228, whole genome shotgun sequence.
ACCESSION   DACECR010000218 DACECR010000000
VERSION     DACECR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.228"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(899..1144)
                     /locus_tag="IWO17_002889"
     CDS             complement(899..1144)
                     /locus_tag="IWO17_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HAQ0291720.1"
                     /translation="MIHVFSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEG
                     YKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          393 a          212 c          158 g          390 t
ORIGIN      
        1 atgttatcag gttcctagcc tttatttaag tattacccca cagacccttc catatagatt
       61 tttaaattac acactagttt aaactaaaac aaacaaggaa aaacttacca tttaatcaaa
      121 aaattagctc atatgaatat attagaatgt aaattagact ggtcaaaatc tagtcaaaaa
      181 agaggacgaa tacaggtagt cctctttttt gttattttga tattctaaat cattttttat
      241 tggattattc ccttgttcga tatagaccca aattgatcta tcaataatta tcaaactgag
      301 tcatatagag acaaaaattt caaaaatgac ctgttaaatg gtcatcgaat atcatttaaa
      361 atcaatacga cggagtcaga cacggagtca aaacaagccc gctgagagta aaaaatatac
      421 agtcccatag gtaaagtgcc acaggactgt atattttttt atttgagatt acctgaaatg
      481 attccattta atttttatct cattttttcc tactagttag tatcaaatac tctcctattt
      541 tctcaataaa ctattttggg ttacatggta tctgaaacag ctaaaacctt gtcattatag
      601 caaaaaatca ctttatatcc gtttcatttc agatacattt cacttaagcg gattccaaaa
      661 aaacaaaaga aaaaggaacc cctagttagc ctatgctttt tattggttcc tctcaaaaca
      721 cggtcatata acaacggggt ggggtaaatg aatgaaaaag gtggaggaaa cattattcaa
      781 tagctgtctt aaatacttcg ttataaaatg tgaacaaata aaagacatct atccctattt
      841 tctacacttc cacttgacta ccatctttga atcgtacaat tttcttaact cttctcgttt
      901 acctcaattc attctcaagt ggaatcaact catcatctaa cattttacgc catgctcgct
      961 tcatttcttg tgggtattta aatggcttcg cttctggatg tttggcttta tatccttcgt
     1021 aatccttact gttgataatt gctttcatat taggaattgg atcttcacct ggtagatatt
     1081 gcagattaga agcatgtaat tctttctcag aaacaaacca ctcattggaa aagacatgaa
     1141 tcaaatcatc act
//
LOCUS       DACECR010000219         1106 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.229, whole genome shotgun sequence.
ACCESSION   DACECR010000219 DACECR010000000
VERSION     DACECR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.229"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..999
                     /locus_tag="IWO17_002890"
                     /pseudo
     CDS             1..999
                     /locus_tag="IWO17_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          391 a          199 c          222 g          294 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      601 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      661 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      721 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt agctgaatca
      781 acttataagt cgttgaaagt agaatttgtc tatcaataca catttgaaac cttacaacaa
      841 ttggatttgg agttatttga ctatgtcaat tggtggaacc accttcggtt gcacggtaca
      901 cttggctacg agacaccggt tggttaccgt aaccagagat tggcgcagcg aatccttgat
      961 aatgagctcg gatgtgctaa cgctagcgag gcagtctaac tttaactgtt agctcctgcc
     1021 gaagatcgtc acatccgagg aggctcattg tcaaggacaa tcggaatagc atacggaaag
     1081 aagttctgca ccttataaaa tttgtc
//
LOCUS       DACECR010000220         1089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.230, whole genome shotgun sequence.
ACCESSION   DACECR010000220 DACECR010000000
VERSION     DACECR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.230"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            414..947
                     /locus_tag="IWO17_002891"
     CDS             414..947
                     /locus_tag="IWO17_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ0291721.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          350 a          203 c          200 g          336 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgttta
//
LOCUS       DACECR010000221         1061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.231, whole genome shotgun sequence.
ACCESSION   DACECR010000221 DACECR010000000
VERSION     DACECR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.231"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IWO17_002892"
     CDS             complement(36..992)
                     /locus_tag="IWO17_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291722.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DACECR010000222         1001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.232, whole genome shotgun sequence.
ACCESSION   DACECR010000222 DACECR010000000
VERSION     DACECR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.232"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            369..1001
                     /locus_tag="IWO17_002893"
                     /pseudo
     CDS             369..1001
                     /locus_tag="IWO17_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac c
//
LOCUS       DACECR010000223          989 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.233, whole genome shotgun sequence.
ACCESSION   DACECR010000223 DACECR010000000
VERSION     DACECR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.233"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            52..>989
                     /locus_tag="IWO17_002894"
     CDS             52..>989
                     /locus_tag="IWO17_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0291723.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTK"
BASE COUNT          355 a          180 c          189 g          265 t
ORIGIN      
        1 gtgctacact caaaatagtt ccgacgaaag aattataagg agtgagtaca aatgacctat
       61 aaccatctta caccaactga actcgtaatg atagaagctt atttcaatca atcacaacct
      121 gtttcaaaag tagccaatct gttacaacgt tctcgacaaa ctatttataa ggtctatcgt
      181 tttcttaaat ctggtggaag cgcaatgagc tactataaac aatataagaa aaacaaacgt
      241 aactgcggaa gacatccgat tgttttacct gatgagcaac aggaatacat tcaaaaaaag
      301 gttgttcaag ggtgggctcc tgatgtcctt attggtcgag cagagtttcc tattgcttgt
      361 tcggtacgca cactgtatcg aatgtttaaa gataaaacct ttgacactca cgatttacca
      421 atgaagggaa aaagaaaacc aaacggtcat aaagaaaaaa gaggaaaaca agggtttcgc
      481 cggtcaatcc gagacagaaa gacagactat acccagtttg atcatgaatt tggtcacctg
      541 gaaggtgaca ctatcgtggg tggaaaacat aaaagcgcgg tcatcacatt agttgaacgg
      601 ctatctaaag tcatcattac cttgaaaccc gaaggcagac aggctaaaga cattgaaaat
      661 aggctgaatc aatggtttcg ttgtgtccca aaaaacttat ttaaatccat cacatttgat
      721 tgtggaaaag aattctcaaa ttggaaaaag attagtaata ccaacgatat ttcaatttat
      781 tttgcggatc caggaacacc ttctcaacga ggattgaatg aacattcaaa tggcttatta
      841 cgtaaagatg gtttgtataa agctatggat tttaatggtg tatctgaaac ctttatccaa
      901 tcggtggcat cgaaacgaaa tcatattcct agaaaatcat tacactatcg gacaccaatg
      961 gaagtatttt tgagttacgt cacaaaaga
//
LOCUS       DACECR010000224          969 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.234, whole genome shotgun sequence.
ACCESSION   DACECR010000224 DACECR010000000
VERSION     DACECR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.234"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            236..937
                     /locus_tag="IWO17_002895"
     CDS             236..937
                     /locus_tag="IWO17_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0291724.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          349 a          164 c          154 g          302 t
ORIGIN      
        1 aaaataaatt atattatcgt tgttagcgtt tactatatgt attattgttg ttttttatac
       61 acaaatcatt cgcttcaaaa caaattattc aataccattg tatatttttt acataataac
      121 aaattatatt tgtaaagtat taaaacttgt ttctaatgtt ggtatgttaa aactttacaa
      181 attctttaaa aaaagtgaca ataaatatag tgaacaataa ggagaggact ttttaatgcg
      241 tgttaataaa atcgattttc acttttcaaa actcagatat caacctgatt ttgaacattt
      301 ctcggatgaa gagttccaac agatcaagga acatactgtt cttcgttctt ataaaaaagg
      361 acagatcctt ttcgacgatg gtgatcccag acaacggttg tatatcttga ccaaaggact
      421 cgttcgttta gaacgttatg atgaaagtgc aacttattac tactatgact atgtgaatac
      481 aaataacctt tttcctatgt caggaatgtt tggagaagag acctacactt atacagctca
      541 agcgatgact gatattgaaa cattctacct tcctgtttcc tactatgaaa aattagcacg
      601 aaaaaatccc gaacagttga tctatatgat acaaaaaatt tctaaagtag ttgaaataca
      661 tgaattgcgt atccaaatcg gattgacctc tagcgccttt gaccgggtaa aacaaaattt
      721 attcattttg aaagaagaat tgggcgtacc aacaaaagat ggtcatatca ccattcctta
      781 tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa ctgctggaca
      841 agtaatcaaa caactacgtg attccgggct gttagattat gacaaaaagc gttttacttt
      901 ctgtaagaca gctactactc cagctatgag agaataacgc gaacaaaaat aagcagtgac
      961 cctgataaa
//
LOCUS       DACECR010000225          932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.235, whole genome shotgun sequence.
ACCESSION   DACECR010000225 DACECR010000000
VERSION     DACECR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.235"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..798
                     /locus_tag="IWO17_002896"
     CDS             4..798
                     /locus_tag="IWO17_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAQ0291725.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKGPSEKRKLNISDYTTQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKREILLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISAINSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
BASE COUNT          389 a          108 c          144 g          291 t
ORIGIN      
        1 aatatgagta aacaatgtgc aaagtgcgga ggtaaaattg gcttaacttc atataaaatc
       61 aaagataaac aaattgtttg cggcaattgt atgaaaaaag ctggttacgg tatgactact
      121 ccttataaaa taataagatc tttgaattta gaagatttag ataagggtcc atcagaaaaa
      181 agaaaattaa acatatctga ttatactaca caaaacaatc atgatatagg taatgataga
      241 atgagaacta ctgaagagat ggtagaattt tgtcttaaat acggctatgg taaaggaatg
      301 actaaaaaat ggacaactca tcattttaac ttaatatcag atcaacttaa cagtgacgaa
      361 tacgtaatct tctgctttgt aggattgcat aactatatct cgtcaactaa gcatgacaat
      421 aattatgcat acgcattaac taataaacgt ttaattgtag ctcaacaaaa aatgataggc
      481 aataatgtac aaagtattat attagacaat ttaaataata ttagtaaaaa aagagaaata
      541 ctgttgggta cattaactat tgatactctt ggtaaagtat tgaatgttgc agttgataaa
      601 gatactgttg ataggataag tgattcttta aacgaaatta tttataattt aaaaagaaga
      661 aatatttctg ctataaattc caatacatca acaatttcct cagcttcaga aataagaaaa
      721 tataaagaac tattggatga cggaatcatt acagaagaag aatttaataa aaagaaacaa
      781 gaattattag atttataata tctatcaaaa aagatgagca tttaatttag ctcatctttt
      841 tctatttaca atacaaacat ttgttcgtat actctttttg aggtgaacat tatgttgatg
      901 gaaggaaaaa cacaattatg gtttaaattt ga
//
LOCUS       DACECR010000226          932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.236, whole genome shotgun sequence.
ACCESSION   DACECR010000226 DACECR010000000
VERSION     DACECR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.236"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>932
                     /locus_tag="IWO17_002897"
     CDS             <1..>932
                     /locus_tag="IWO17_002897"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0291726.1"
                     /translation="PEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVD
                     LQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGE
                     FYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQEL
                     NTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSAD
                     TTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIR
                     QKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKK"
BASE COUNT          294 a          176 c          206 g          256 t
ORIGIN      
        1 caccagagta tcttttcaac gtcttagcgg atcaagcaaa acaagcaacg ttccaattga
       61 acgatctaaa caaagccttt gtgcaattgg catctaccta caatcaattt aatgggttat
      121 ttgatgatgt ggatctccaa tcaaaaaaac taggaacaga tgagcaacaa cgaaatgtga
      181 cgattaccga agtcatcaag aaattgaatg atgtagatgt tttagaacac gatggggacg
      241 tgatcgggga tgcgtatgag tttctgatca gtcaatttgc ttcagaagca gggaagaaag
      301 ccggagaatt ttacacacct catatggttt ctgatatgat ggcgcaaatc gtaacgctcg
      361 atcaaaagga acgacgattc tttagtgttt ttgatccaac gatgggttca ggttcattga
      421 tgttaaacgt acgtaactac ttaacacacc cagataacgt taaatatcat gggcaagaac
      481 taaatacaac gacatacaat ctagcaaaaa tgaacttgat tttacatggt gtcgacgccg
      541 aagaaatgaa tctccgcaat ggggatacgt tgaataagga ctggccaaca gatgaacctt
      601 acacatttga tgcagtagta atgaacccac cttattcggc taattggtca gcagatacga
      661 ctttcttaga tgattctcgg tttaatcgtt atgggaaatt agcgccaaaa tccaaagcag
      721 attttgcgtt tcttcttcat gggttctatc atttgaagga aacaggtacc atggccatcg
      781 tcttaccaca cggagtttta ttccgtggcg ctgcagaagg ggtcatccgt caaaaattac
      841 tagaagatgg cagtatttat gctgtgatcg gtatgcctgc gaacttgttc tttgggacat
      901 cgattccaac gacggtgatt gtgttgaaga ag
//
LOCUS       DACECR010000227          832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.237, whole genome shotgun sequence.
ACCESSION   DACECR010000227 DACECR010000000
VERSION     DACECR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.237"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>832
                     /locus_tag="IWO17_002898"
     CDS             <1..>832
                     /locus_tag="IWO17_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0291727.1"
                     /translation="RDRRFDVTLLINGLPIVQIELKQVTAKDGVYQAFNQIKKYAEEG
                     MFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKFLFSWRTKDNEKVENLYEFCKQV
                     LNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAIQALFIAANKHQSGYVWHATGSG
                     KTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTTEFTKFASEFNTGISSGNAKANS
                     LIVGTGSAKELSETLLADANANVVIITTRQKLDAALKYAKKQEEKKGTNRFQKLMGQH
                     I"
BASE COUNT          286 a          152 c          171 g          223 t
ORIGIN      
        1 cgggatcgac gttttgacgt caccttatta atcaatggat taccgattgt ccaaatcgag
       61 ctgaaacaag taactgcaaa agatggtgtt tatcaggcat ttaaccaaat caaaaaatat
      121 gcagaagaag gtatgtttcg gaacaatatc ttttcgacct tacagttgtt tgtggtttcc
      181 aatgaacaaa ccactcggta ttttgcaaat gccttaccga aagacttgca ccctaagttt
      241 ttatttagtt ggcgaacgaa agacaatgaa aaagtagaaa acttatatga gttttgtaaa
      301 caagtgttaa atatcccgga tgctcatcgt ttgattgcgg attatacaat cgtcagtgaa
      361 gatcaagaca ataaaaccct catggtgtta catccttatc aagtacatgc gattcaagcc
      421 ttgtttattg ccgcaaataa acatcaatca gggtatgtgt ggcatgcgac tggttcagga
      481 aagacgttga ctagttttgt atctactaag cttttagcac gaaaatcggg gatcgatcga
      541 acgatcatgt tagtggaccg caaagattta gataatcaaa ccaccacaga gtttacaaaa
      601 tttgcatcgg aatttaatac tgggatttct tccgggaacg ccaaagcgaa tagcttgatc
      661 gttggaacgg gaagtgccaa agaattaagt gaaaccctgt tggcagatgc caatgcaaat
      721 gttgtaatca ttacaacccg tcaaaaacta gatgcggcgt tgaaatatgc aaagaaacaa
      781 gaagagaaaa aaggaaccaa tcgcttccaa aaattaatgg gccaacatat cg
//
LOCUS       DACECR010000228          823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.238, whole genome shotgun sequence.
ACCESSION   DACECR010000228 DACECR010000000
VERSION     DACECR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.238"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            55..369
                     /locus_tag="IWO17_002899"
     CDS             55..369
                     /locus_tag="IWO17_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291728.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            362..>823
                     /locus_tag="IWO17_002900"
     CDS             362..>823
                     /locus_tag="IWO17_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0291729.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEK"
BASE COUNT          287 a          118 c          192 g          226 t
ORIGIN      
        1 acgcgagaac gttgattcga aaggtcgtca atttaaacat tgggagaaga aataatggag
       61 gaactaatca caaaagtaga gcagtgggca aaagataagg gattggatca agctgattcc
      121 agcaagcaaa tgttaaaaac gatcgaagag attggggaag ttgccgcttc tctagctaga
      181 aaagatgaat atggtttaag agacggaatt ggagacgtag tagtaacctt gattatttta
      241 gctatgcaaa atgatatgga tttgtacgag tgtctgaacc aagcatacaa cgaaatcaaa
      301 ggacgcacag gaaaaatggt agatggtgta ttcgtgaagt cgagtgattt ggaggacagc
      361 gatgaataaa aaagtattaa ttgataaaca agcattgatt gatgaattaa tgaaaatacc
      421 tggtgtggga tctaatagtg acgctttaga aacgattaaa cgtttcccat cgtatgaacc
      481 gcagaagcca gttgtgccta agtttgtggc ggaactactc gactattatc gtcagtcaac
      541 agatgttgat ttattagctt tattgataac tttccatgat tggtattatc gcaaaactaa
      601 agacggtgag catgaagaag caattgattg gctagttgat catcctgaaa tttatatgcg
      661 tgcatggctg tttggctacg agctcgagaa agagccgttg tattgcgtaa taatcaatgg
      721 tagatatctt gtcaaagtat tcagcaatac aaacgttgtc atattagttc cagcagatga
      781 attttcacag tacgttactc aaaaatttca attaactgaa aaa
//
LOCUS       DACECR010000229          793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.239, whole genome shotgun sequence.
ACCESSION   DACECR010000229 DACECR010000000
VERSION     DACECR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.239"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..782
                     /locus_tag="IWO17_002901"
     CDS             <1..782
                     /locus_tag="IWO17_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0291730.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          244 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc ttt
//
LOCUS       DACECR010000230          736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.240, whole genome shotgun sequence.
ACCESSION   DACECR010000230 DACECR010000000
VERSION     DACECR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.240"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..734
                     /locus_tag="IWO17_002902"
     CDS             <1..734
                     /locus_tag="IWO17_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ0291731.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQ
                     YQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIR
                     WYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          318 a           94 c          122 g          202 t
ORIGIN      
        1 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
       61 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
      121 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
      181 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
      241 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
      301 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
      361 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
      421 agggaactga gggttcactg attctacatt cagatcaagg atggcagtat cagatgccac
      481 aatatcaaaa aaaattaaaa gagaataaca ttattcaaag catgtctaga aaaggaaatt
      541 gcttagataa ttctgtaata gaaaattttt ttggtgtgct taaatcagag tttttttatc
      601 gagaaaaatt ccgatcaatt gagatatttc aaagtaaatt aaatgagtat attaggtggt
      661 ataataacaa aaggataaaa ctaaagttaa atggactatc tccagtagaa tatcggaaac
      721 agtccattaa atagtt
//
LOCUS       DACECR010000231          719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.241, whole genome shotgun sequence.
ACCESSION   DACECR010000231 DACECR010000000
VERSION     DACECR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.241"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(94..465)
                     /locus_tag="IWO17_002903"
     CDS             complement(94..465)
                     /locus_tag="IWO17_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291732.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(467..>719)
                     /locus_tag="IWO17_002904"
     CDS             complement(467..>719)
                     /locus_tag="IWO17_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291733.1"
                     /translation="ITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFF
                     VIKTTSELFSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
BASE COUNT          236 a          119 c           84 g          280 t
ORIGIN      
        1 tcggcgttat gactatataa agtatacttt cgttgatcca gaaagataca aagattaaat
       61 aaaaaaataa caaacaaact cgcttgaaat tcattaggtg agtttgttta ttattttttc
      121 tgcttctcga ttcatcagcg ccatatctgt tttcgtaatg gtttcattag gaatatagtc
      181 agcacatttt cttttctcta acattctata cccactatta tgtattccta aggtattcaa
      241 ttccttaact gttgaaaaca aattccaaac ggtttggtgg cttccttttc cgctacttcc
      301 attataaata atatcataat tttctttcaa atactcatca gattttttaa aagcactgta
      361 gtatgctctt gaaacagcag ttcttctata agattcttta gaattatatt tatcatctga
      421 ttcaatttgt ttggaaatag ataaatattc attaaagtca aacattttat tcccccaaag
      481 ttgctactac agatttcccc atatatttat agtcagataa tacattatcg agagtatcca
      541 aaacatcgta atctgaggaa aacaattcag atgttgtttt gataacaaag aaatacaagt
      601 ttaaatcttc atcatatttt atattgattt cagcttttct ttttaaaacc gtatttatca
      661 catcaaaaat tttgccatca ttacttgaaa ttgtgttaat ctcatttttt gtagtaatt
//
LOCUS       DACECR010000232          697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.242, whole genome shotgun sequence.
ACCESSION   DACECR010000232 DACECR010000000
VERSION     DACECR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.242"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>697
                     /locus_tag="IWO17_002905"
     CDS             <1..>697
                     /locus_tag="IWO17_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0291734.1"
                     /translation="WSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGF
                     DAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLY
                     SEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNR
                     AYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIE
                     FYSENTTKLYDIDS"
BASE COUNT          246 a          118 c          150 g          183 t
ORIGIN      
        1 cctggtcact agcagacatt gagcgttata acggagacat caataatcgt ttggcacgaa
       61 aaagagcgga attcaaacaa tttgggaaac aaatcgattt agtaatcgta gtggatcgtc
      121 tattgacagg ttttgacgcc cctacgattc aaaccctatt tgtggatcgt aatctagagt
      181 atgcaggatt gatccaagcc ttttcacgta cgaaccgtac gtatcctgaa aaaacaaaag
      241 gactaattgt tactttccga aagccagcga caatggagaa gaatgtcgag gatgcgacaa
      301 aactgtattc agaagctaaa gaagaatcgg gtttgattta tccaagttac caagaatcca
      361 aaaaaagatt caagcaagca tatggtaagt taaaagaggt ttcatccatg gaatccattg
      421 atgaacatac gccacttgaa atccgtatcg agtacgtcaa agcatttcaa gaattgaatc
      481 gtgcgtatga atcgttagtg acttatgatg attacaatga tgacatggtg caatcttcaa
      541 ctttgaacaa tcaaattcaa cttttggaag aacaaattgg cgtgtacgag accgtgaagg
      601 gttcattgat ggaggaagaa ccagaaaaag agggagaaga tttttcaacg atcgaatttt
      661 atagtgaaaa taccactaag ttgtatgata ttgattc
//
LOCUS       DACECR010000233          693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.243, whole genome shotgun sequence.
ACCESSION   DACECR010000233 DACECR010000000
VERSION     DACECR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.243"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            15..284
                     /locus_tag="IWO17_002906"
     CDS             15..284
                     /locus_tag="IWO17_002906"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291735.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            414..>693
                     /locus_tag="IWO17_002907"
     CDS             414..>693
                     /locus_tag="IWO17_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291736.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDME"
BASE COUNT          270 a           95 c          166 g          162 t
ORIGIN      
        1 gaggtggtag aaggatgagc gacccataca ttactaaatt agaaaagttg tccgaagatt
       61 ttgagaagaa taaaaataaa ctaactaaag ataaacttat agaactggtt aggttggtta
      121 tcagaggagc tattacaaga gaaaaggcgc agcaaaacat agaaattttt gctcgtagtg
      181 tccgagatgg attagagaag aaagttaaac aaaatgaaca acagacaccg cagaataaca
      241 aaactaagaa aacgggaact gaatgtacta aagacaaagt ttgaaaaaga atatggaatt
      301 tcagcagaag aaacatataa agtggcaagt cagtgtgttg ctgatgcgag cggcgctatt
      361 cgtaagtttg gaatttcgat attaagtgat gatcgtaaat gggaggaaaa agaatgaaac
      421 taaaagacgg attttactcc agcagtcatg gtatcggcgg tttaatgcta gatatgccga
      481 caaagaaccc taaaacacgt gagaaaccaa aattcaaagt cggtgacatg gttcgctgtg
      541 aagcagaagg attcatctat ccattttgtg gatatgtaga acatctctat aatcactcag
      601 caatcattcg tattgaaaac acgatggaat gtgacaagtg gttagcgaaa agcaaagaga
      661 atttagcagt agcgagattg gtggatatgg agg
//
LOCUS       DACECR010000234          618 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.244, whole genome shotgun sequence.
ACCESSION   DACECR010000234 DACECR010000000
VERSION     DACECR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.244"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>618
                     /locus_tag="IWO17_002908"
     CDS             <1..>618
                     /locus_tag="IWO17_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291737.1"
                     /translation="SGKSLEALELEQEEIRRFQNCSLYPVYHGSAKSNIGIEQLIEVI
                     TNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKIT
                     EMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTV
                     EPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQM"
BASE COUNT          223 a          100 c          123 g          172 t
ORIGIN      
        1 tccggtaaat cattagaagc attagaactc gaacaagagg aaatcagaag atttcagaat
       61 tgctccttgt accctgttta tcatggaagc gcaaaaagca acatagggat tgagcagctt
      121 atagaagtga taacgaataa attttattca tcaacataca gaaagaagtc tgaactttgc
      181 ggaaatgtct tcaaaattga atattcggaa gaaagacaac gtcttgcata tgtacgcctt
      241 tatggcggaa tcctgcattt gcgggattcg gttagaatat cggaaaagga aaaaataaaa
      301 attacagaaa tgtatacttc aataaatggt gaattatgta aaattgataa ggcttattcc
      361 ggggaaattg ttattttgca aaatgagttt ttgaagctaa atagtgttct tggagataca
      421 aagctattgc cacagagaga gagaattgaa aatccgctcc ctctgctgca aacaactgtt
      481 gaaccgagca aacctcaaca aagggaaatg ttacttgatg cacttttaga aatctccgac
      541 agtgacccgc ttctacaata ttatgtggat tctacgacac atgaaattat actttctttc
      601 ttagggaaag tacaaatg
//
LOCUS       DACECR010000235          598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.245, whole genome shotgun sequence.
ACCESSION   DACECR010000235 DACECR010000000
VERSION     DACECR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.245"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(12..494)
                     /locus_tag="IWO17_002909"
     CDS             complement(12..494)
                     /locus_tag="IWO17_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291738.1"
                     /translation="MNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVSIPLLIFLTNQ
                     EQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGNALRAAHEINK
                     WFPEYKNFNFLNKLDLGISSFGKPFFLALCQMMLVKRNLRIIWTEQKEENLFLFCLFG
                     "
BASE COUNT          225 a          100 c           74 g          199 t
ORIGIN      
        1 gcaaaacatt tttaaccaaa taaacagaac agaaagaggt tttcttcttt ctgttctgtc
       61 caaattattc taagatttct ctttaccaac atcatttgac aaagagccaa aaaaaacggc
      121 tttccaaatg atgaaatacc taaatctaac ttattaagaa agttgaaatt tttatattca
      181 ggaaaccatt tattaatttc atgtgcagca cgtaatgcat tccctggagt tgtcaaagag
      241 aaaattaatt ctgctaagct tattattgaa aatactgata acttataatt atatcgtcca
      301 ttgacaatca aatataaact aactatactt gttagaacaa aaaaacaggc gttgacttgt
      361 tcttgattag ttaaaaatat caataaagga atacttaccc aatatatttt tctatttatt
      421 tcttctcctc ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa tgctactggc
      481 cactgatagt tcaaggtggt tgctctccaa ccggtttccc ccattactgt caatggtatt
      541 aaacagtata tagagcaaac caataataaa tttttaacat ttattttctc aaaaacat
//
LOCUS       DACECR010000236          591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.246, whole genome shotgun sequence.
ACCESSION   DACECR010000236 DACECR010000000
VERSION     DACECR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.246"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(316..579)
                     /locus_tag="IWO17_002910"
     CDS             complement(316..579)
                     /locus_tag="IWO17_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAQ0291739.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          224 a          103 c           78 g          186 t
ORIGIN      
        1 cttccttttt tgtatggatt ttttcattta cacaaaatat tttatactct cctgaatttt
       61 ttcatgttga aaacatcact aatcaaaggc aaattataat ttagaaatag aatgaaaaca
      121 ctaaatgaaa atagaacatt agaaaaaata gaatcaaaaa aacccaagac tagtgccgaa
      181 ataagggaaa caatcgaagc tacaaaaaat aatgatttcg ttgaacagga ccaatttatt
      241 tgtttccgat atttctcaaa aagaactttc tttgggaaag ttaaaggcga ctgtaaagtc
      301 gcctttagtc cttttttacc aagctgctgc atatttagct ccatattttt taattaatga
      361 tttggctgta gcaacaatac cagctgtaat taatccgcct ccaacaactg cagcaacaat
      421 agaaatgatt gtagcaaccg tgctaccagt attaattatg tcaataactt ttttagctgc
      481 ataagaagaa attccaatgt tagcacttag cattggtaca ccaacaaaga atcccgcaaa
      541 acctaaaatt ggtaaaacta ataataaatt ctttttcata ataattcctc t
//
LOCUS       DACECR010000237          580 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.247, whole genome shotgun sequence.
ACCESSION   DACECR010000237 DACECR010000000
VERSION     DACECR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.247"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(65..150)
                     /locus_tag="IWO17_002911"
     tRNA            complement(65..150)
                     /locus_tag="IWO17_002911"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="IWO17_002912"
     tRNA            complement(167..238)
                     /locus_tag="IWO17_002912"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="IWO17_002913"
     tRNA            complement(250..322)
                     /locus_tag="IWO17_002913"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="IWO17_002914"
     tRNA            complement(340..421)
                     /locus_tag="IWO17_002914"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="IWO17_002915"
     tRNA            complement(429..501)
                     /locus_tag="IWO17_002915"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="IWO17_002916"
     tRNA            complement(505..577)
                     /locus_tag="IWO17_002916"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          146 a          168 c          137 g          129 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg
//
LOCUS       DACECR010000238          536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.248, whole genome shotgun sequence.
ACCESSION   DACECR010000238 DACECR010000000
VERSION     DACECR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.248"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..>536
                     /locus_tag="IWO17_002917"
     CDS             51..>536
                     /locus_tag="IWO17_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAQ0291740.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YL"
BASE COUNT          194 a           84 c          108 g          150 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctg
//
LOCUS       DACECR010000239          530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.249, whole genome shotgun sequence.
ACCESSION   DACECR010000239 DACECR010000000
VERSION     DACECR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.249"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>530
                     /locus_tag="IWO17_002918"
     CDS             <1..>530
                     /locus_tag="IWO17_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0291741.1"
                     /translation="PENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEH
                     ENTVEPTSLENKIYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHI
                     QKVIHKMFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKE
                     FPNQLIREGRTMEDPD"
BASE COUNT          193 a          100 c          111 g          126 t
ORIGIN      
        1 cctgagaatc ggaaacaagc caaagggcaa ttggcacgta caacccatga tcagtatgga
       61 gaggtcttgc atacctatac cattaagaat gcgctagaag atggctctgt cttaggattt
      121 caagtagagc atgaaaacac cgtggagccg acttcactag agaataaaat ctatcggaaa
      181 ttaaaagaag tagaaacata tgcagagtat tcatcagaac agatcaatcg tatgattgat
      241 caaatggaac ctgtcaaaaa agaaagttat ttggacccag ctgtttttga agcagatgaa
      301 catatccaaa aagtcattca taaaatgttc cgtccagata atgcctatac aaaatttgat
      361 tttcgaaatg gcaggccaac gaaatctgca atcttaacca cgagttccat tgacatggcc
      421 aaaaagtatt atcgggccat caaagagatg acgaaagagc ctgattggtt aacaaaagag
      481 tttcccaatc agctcattcg tgaaggacga acgatggagg atcctgattt
//
LOCUS       DACECR010000240          529 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.250, whole genome shotgun sequence.
ACCESSION   DACECR010000240 DACECR010000000
VERSION     DACECR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.250"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            32..303
                     /locus_tag="IWO17_002919"
                     /pseudo
     CDS             32..303
                     /locus_tag="IWO17_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876065.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          198 a           81 c           93 g          157 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttaaatcta tattttgata tacaaattac
       61 agcttcagat actaagttgc ataaaccttt tccggacccc atacaaaagg caattgatga
      121 attgggcata aaaaataaga aacgatttat attggtgatt cattatatga catgcaaagt
      181 gcgaaagctt gtgggatagc atttggttta gctaaatggg gagccaaaaa tatagaaaat
      241 tttcaaagtg tagatataat tattgattct cctcaagaaa tacttgattt gttagaaaaa
      301 tagacaaaga gcgtgagata aggatagggt ctcacgctct ttgtttttcg ccatttaaag
      361 attgaaattt ttgccatcgc cgattgtaaa attaagctag acaactaaaa aagtcatttg
      421 tgctacactc aaaatagttc cgaccaaaga attataagga gtgagtacaa atgacctata
      481 cccatcttac aacggatgaa cttgtaataa tagagtctta tttcaaaat
//
LOCUS       DACECR010000241          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.251, whole genome shotgun sequence.
ACCESSION   DACECR010000241 DACECR010000000
VERSION     DACECR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.251"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..400
                     /locus_tag="IWO17_002920"
     CDS             <1..400
                     /locus_tag="IWO17_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0291742.1"
                     /translation="EPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYY
                     FVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNNKI
                     KLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT          168 a          110 c           97 g          153 t
ORIGIN      
        1 ggagccactc aaacggcatt atcatcgact gtttaaacga ccaattagtc aaacagaaat
       61 cgttgatgag ctgttaagct acaacgaaga attgcgaact gcgtatcatt tttgtcaatt
      121 attacgctat tatttcgtta aacgaggtac ggaaggcttt caagaaaccc ttaaaacaat
      181 ttcatctacg ttacctcagt cgtttaaaaa gaaattcacg atcttccatt gctaccaatc
      241 gggtatttca aatgccttta aggtgtccca ctcaaacggc gtcactgagg ggctgaataa
      301 caagatcaag ctgattaaac gaattgcgtt tggttatcgc aacttttata actttcgggc
      361 acggatctat ttacaacaag gtctgatctt tgaaaactaa aaaaatatgc ggcgcacaga
      421 acgcgccgca tatcggacta agttttattc aaatttgtct tcaccaacac gatttgacga
      481 agagccaaaa aaagttacga cagttctatg tcgtagctct ttttttcg
//
LOCUS       DACECR010000242          525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.252, whole genome shotgun sequence.
ACCESSION   DACECR010000242 DACECR010000000
VERSION     DACECR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.252"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            29..>525
                     /locus_tag="IWO17_002921"
     CDS             29..>525
                     /locus_tag="IWO17_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ0291743.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          192 a           97 c           86 g          150 t
ORIGIN      
        1 accaattcac agaaaagagg acttccctat gaatgatttt actacagaaa ttgtgcaaac
       61 tctagtcact aaaggcgatt taaatgaatt attccgttcg cacttagaaa aagcgataaa
      121 cacactccta cggactgaat taacggcttt tttagattac gaaaaatatg atcgcactgg
      181 ttttaattca ggtaattcga gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg
      241 tgaattaaca ttggaaatac ctagagatcg taatggtgag tttaaacaac aaactttacc
      301 agcctacaaa agaacaaacg atacattgga aaccactatt atccatttat tcgaaaaagg
      361 tgttacgatg tctgaaattg ctgatttgat cgaaaaaatg tacggtcatc actatactcc
      421 acaaaccatg tccaacatga ctaaagttct gactgaagaa gtaaatgcct ttaaatccag
      481 agccttaaat gataagtatg tcgctatttt tatggacgct actta
//
LOCUS       DACECR010000243          493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.253, whole genome shotgun sequence.
ACCESSION   DACECR010000243 DACECR010000000
VERSION     DACECR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.253"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            8..322
                     /locus_tag="IWO17_002922"
     CDS             8..322
                     /locus_tag="IWO17_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291744.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          164 a           58 c           75 g          196 t
ORIGIN      
        1 atagcttttg gatttttaca gtaattttat cttaataata gcaatattat tacttctaaa
       61 tatttggttt tttgataaga gtagaaatgc agggataggt tttagaacca aacgaagtac
      121 gtcttctgaa aaaaagtggg tatattcaca gactattttt tacggagggg ttatttcaat
      181 cagtttactt tcttcaacac tttattcttt taatgttata gacgtttcca tgtcaaattt
      241 tatatctatt attggaatcc ttatttctgc aattattaca caactacttc ttgtgtttga
      301 agagaagtca aaaaataatt aaagcaatta tgccttttag tacaggaatt attacttctt
      361 acaaatgtat agcgaatgtg aagcctgctg ttttaattgt tctagaaaaa cttttgagtg
      421 aacaattatt ttatgttagg atgaattaat ttctctagag tttataatta aaaaacttat
      481 ttgaaggaac atg
//
LOCUS       DACECR010000244          491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.254, whole genome shotgun sequence.
ACCESSION   DACECR010000244 DACECR010000000
VERSION     DACECR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.254"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(21..251)
                     /locus_tag="IWO17_002923"
     CDS             complement(21..251)
                     /locus_tag="IWO17_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291745.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT          164 a           68 c           64 g          195 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg t
//
LOCUS       DACECR010000245          476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.255, whole genome shotgun sequence.
ACCESSION   DACECR010000245 DACECR010000000
VERSION     DACECR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.255"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..>476
                     /locus_tag="IWO17_002924"
     CDS             22..>476
                     /locus_tag="IWO17_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0291746.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKK"
BASE COUNT          178 a           87 c           95 g          116 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggcattcaaa agaaatattt ctgaacgtaa aaaaga
//
LOCUS       DACECR010000246          472 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.256, whole genome shotgun sequence.
ACCESSION   DACECR010000246 DACECR010000000
VERSION     DACECR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.256"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(35..319)
                     /locus_tag="IWO17_002925"
     CDS             complement(35..319)
                     /locus_tag="IWO17_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ0291747.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
BASE COUNT          134 a          107 c           72 g          159 t
ORIGIN      
        1 agaggttgtg acacgtcttt tgtcacaacc tcttttattt ttgcttcact gtaactgcta
       61 caatatcacc gatttctttt ccgatgtttt tacgaatatc ttttcgtagt ccaaggatgt
      121 agcagactga accatctgga tttttcacgc ccatattcac gatactgcct tcatatggca
      181 cattgtcaaa agttgcttgg actttaactc ttccagcacc gaattctttt cttatatcat
      241 acggaaaaat aatatatgcc ccgcccttgc cgacttcgga agcatagatt tttgattcaa
      301 aagaataaat ttccctcatt ttatttctcc tattttttac atctagtata gcatagcaaa
      361 aaaaagaagg aactgtgaca aaagtcttgt cgcagttcct tttttcgaat aaacggtgtt
      421 ctcctgccaa ctcctcggtc acaagtcaca atagtcaaca aacatgagaa gc
//
LOCUS       DACECR010000247          468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.257, whole genome shotgun sequence.
ACCESSION   DACECR010000247 DACECR010000000
VERSION     DACECR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.257"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>468
                     /locus_tag="IWO17_002926"
     CDS             <1..>468
                     /locus_tag="IWO17_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0291748.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DACECR010000248          425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.258, whole genome shotgun sequence.
ACCESSION   DACECR010000248 DACECR010000000
VERSION     DACECR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.258"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(87..>425)
                     /locus_tag="IWO17_002927"
     CDS             complement(87..>425)
                     /locus_tag="IWO17_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0291749.1"
                     /translation="LERMAGRSLIDIKSPIITDMPKAPKHGNKAEDAIIQMMDIEAER
                     DAILAALMALSLISRQILYYSFCVPDSFSNYRISREVGYSERSIQRMKSEALIEFAEA
                     YKHGRIIAYK"
BASE COUNT          118 a          113 c           67 g          127 t
ORIGIN      
        1 agacctatca ctaatctttc gacactacca taatatcact ggtaaatagc taaaaaccgc
       61 catcattccg ccaaaaagcc gccaaattat ttataagcaa ttattcttcc gtgtttatat
      121 gcttctgcaa actctattag agcttccgac ttcatccgtt gtatacttct ttctgaataa
      181 cccacttcac ggctaattct gtagtttgag aagctatctg gcacacaaaa gctatagtag
      241 agtatctgac gactaatcag actaagagcc atcaaggctg ctagaatcgc gtctctctcc
      301 gcttctatat ccatcatctg aatgattgcg tcttctgcct tattgccgtg cttcggcgcc
      361 ttcggcatat ccgtaataat cggcgactta atatctatca aagagcgacc tgccatccgc
      421 tccaa
//
LOCUS       DACECR010000249          399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.259, whole genome shotgun sequence.
ACCESSION   DACECR010000249 DACECR010000000
VERSION     DACECR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.259"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          111 a           98 c           76 g          114 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttgg
//
LOCUS       DACECR010000250          394 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.260, whole genome shotgun sequence.
ACCESSION   DACECR010000250 DACECR010000000
VERSION     DACECR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.260"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..285
                     /locus_tag="IWO17_002928"
     CDS             <1..285
                     /locus_tag="IWO17_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0291750.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          117 a           73 c           92 g          112 t
ORIGIN      
        1 gaggttgcag aagatgatcc ttattgggaa atcgaagcct cctatattgc tcaatgtgcc
       61 tacaatacaa ccttgatgtt ttctccagat gtgatcattt ttggcggtgg tgtgatgaaa
      121 caacgccata tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa tgggtatgtc
      181 gaaacacctg agttaagcaa atatatcgtg accccagcat tagaagacaa cgctggaacg
      241 ctcggttgtt tggcattggc tcgagaagcg gtcttacatt cataaacgaa aaaaggctct
      301 tcgacattta gtgttggtag agaaagatac aactgaaaat ttagtcataa ccagttaggc
      361 agagtgctta actggttttt gttttacttg gaag
//
LOCUS       DACECR010000251          381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.261, whole genome shotgun sequence.
ACCESSION   DACECR010000251 DACECR010000000
VERSION     DACECR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.261"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          176 a           49 c           58 g           98 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
      241 aaaaaagtta ttgacatgtt actaacgaca tgataagata actgagtcgt taaaaaagaa
      301 ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
      361 acgataagtt atgataaatg a
//
LOCUS       DACECR010000252          374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.denovo.262, whole genome shotgun sequence.
ACCESSION   DACECR010000252 DACECR010000000
VERSION     DACECR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.denovo.262"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>374)
                     /locus_tag="IWO17_002929"
     CDS             complement(<1..>374)
                     /locus_tag="IWO17_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0291751.1"
                     /translation="IEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR
                     FEGVTGRKISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKC
                     ITKRVCCICGKTGADIDHFDKAL"
BASE COUNT          108 a           72 c           75 g          119 t
ORIGIN      
        1 agcgctttgt caaagtgatc gatatcagct cctgttttgc cacagatgca gcagactctt
       61 tttgtaatgc atttgtagaa gtaatactct tgattcgctg gtaaaatctc atatccttct
      121 ttgaaaggaa tatgatgttc aaagatgaaa tctaggatga tattcgctaa gatattggca
      181 tcactcacgg ttgtattcga ttcgtctttc aggcttattt tgcgccctgt gacaccttca
      241 aaacgaaagt agaagaattc cttccagaag tccgttggca tgcctgtatc gataaaaata
      301 tcgcctatca gcgcatagat gaagtttcgt tgctgtacgg tgaaacgtct aggatcaata
      361 aaacgaattt caat
//
LOCUS       DACECR010000253         9011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.guided.103, whole genome shotgun sequence.
ACCESSION   DACECR010000253 DACECR010000000
VERSION     DACECR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.guided.103"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(101..544)
                     /locus_tag="IWO17_002930"
     CDS             complement(101..544)
                     /locus_tag="IWO17_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0291752.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(739..2079)
                     /locus_tag="IWO17_002931"
     CDS             complement(739..2079)
                     /locus_tag="IWO17_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0291753.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2361..3257
                     /locus_tag="IWO17_002932"
     CDS             2361..3257
                     /locus_tag="IWO17_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0291754.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(3400..4341)
                     /locus_tag="IWO17_002933"
     CDS             complement(3400..4341)
                     /locus_tag="IWO17_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0291755.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4467..5588
                     /locus_tag="IWO17_002934"
     CDS             4467..5588
                     /locus_tag="IWO17_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0291756.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            5846..7348
                     /gene="lsa"
                     /locus_tag="IWO17_002935"
     CDS             5846..7348
                     /gene="lsa"
                     /locus_tag="IWO17_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0291757.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            7415..7942
                     /locus_tag="IWO17_002936"
     CDS             7415..7942
                     /locus_tag="IWO17_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0291758.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            8154..>9011
                     /locus_tag="IWO17_002937"
     CDS             8154..>9011
                     /locus_tag="IWO17_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0291759.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRN"
BASE COUNT         3002 a         1650 c         1808 g         2551 t
ORIGIN      
        1 cacaacctct gattcacggt gttccagaag tagcttcttc ttatttcttg aagttgatca
       61 cttctggaac atcctctttt tttatttgga tggataatgg tcattccatt tctttgatca
      121 tttttttcat tttgtcgatt tcttcttctt gtgcctgaat gatttcttcc gctaattttt
      181 ttacttcagg atcttttaaa tcagctcgtt tacttgtcaa gatagcaata gagtgatgag
      241 ggatcatcgc cttcatccac gccgtatcgt tgacagttgt ttggcttcgt accataaata
      301 aggaaccggc aaaaacaaga agactagcgg ctaaaatcat agcatttatc tttttgtttt
      361 catacatttt ccacatgaac aacaacatga tgactgccat gactgcaccc atcatcaacg
      421 ccatgaatac acgtgtgcga ctgaagaaga tatgatcaag cgcataaaca ttcaaataca
      481 tcataccaaa catgactacc gtcgaaacac caatcatcaa caaaaacttc caatactttt
      541 tcatttaaaa caaactcctt tttcttcatt cctttcttct atcatgtcat gagcgtggag
      601 aacaatcaat taatctgcac agatggtttt tgaacaccgt ttattcggaa aaaagaactg
      661 caacaatttt tgtcgcagct cctttttttc aatagctcca gatattcaat ataaatagaa
      721 taaaattctc tattggtttc attcattcgt caagacgaaa gcatcattct ttttttgata
      781 actgaaataa atgaacaggt aaagcaaacc ggtcaataca gttaccccaa tggagttcgt
      841 gaataggata ccaaggaaaa tcaaccataa gactgctaat gcaaaaaagc tgattccttt
      901 gtcccataac gttcctttcc ttagtgtcag tacaaaggac gcagcactga taatgatcca
      961 gatcaataat actgtatagg aaagagaacc tgccagatag ccaaataatt tgtcaccgac
     1021 aaaataagaa agagccacac ctagataaag aacactggta caaaacagca cagcacgttg
     1081 cggtacttga tgcttgttta aaacagctag ttttctagag gaacctttcc gatctttcaa
     1141 gcggaagaac aaaattctcg aagaagcata tacacctgaa ttgatagacg agaacagagc
     1201 aagaacaatc acgaaattga cgatatctgc cgcaaatgga atattcattt tttcaaatac
     1261 catcacaaaa ggactaacgt tcgtgcctgc taaagtattc caaggaaaaa taatcaaaag
     1321 caaaaataaa ggaataatgt aaaaggaaat gattcttccc atgacgcctt tgattgcttt
     1381 aggaatcgca cgtttaggat catctgcttc gcttactgtg atagcaatca actcagaacc
     1441 gccgtaagaa tagataacta caagcaaaga actgatgatt cccttcatac cgtggggagt
     1501 aaaaccacca tgatcagtta attgttgaag ggtagggaaa acaccagtac ccaacatttg
     1561 ctgaccgaca agataagcag caaaaatgat caataggata atgacactga ttttagcaaa
     1621 ggccagccaa tattctgtct ctgcgaataa tcgtaccgaa taaagattga tgatagtagt
     1681 taaaacagcc aacataaaaa caaataccca agctgggatg tgggggaacc acaactgtaa
     1741 aaaggaagca gctgcaacag cttcagcaat gatattgata atccacatgg accagtagac
     1801 ccaatcggta aaatcggcag catgagcacc tagatatgga cgaatcaatc cagataatcc
     1861 gtggtgcgta tcaccaccac ttaaaacaat ttttcccaga cctttcatca cgatatataa
     1921 aattatgccg ccaatcaaaa aagcgatcaa tacagaaggg ccggcgattc caatcgcttc
     1981 gccacttcct ttaaatagac ctgcaccaat cgctccacct aaagctagca tggtgatatg
     2041 tcgggagcta agttgttttt tcaatttttt atcttccata aataacctct ttcttttaaa
     2101 ttcacaatca aaatgcatag taagaataaa tagtaagaat aaatagtaat aataataatg
     2161 actaatgata acaaattttc agataattac aactattcag aaaacttttg ggcatatttt
     2221 aatggaagaa tgatttattt catcgtattt tcatagaaat gttaaatttt tataaaaata
     2281 ataggaagcg ttttactggc tttttactat ttaaacaggc tatcatagga aaaagtgaca
     2341 gagaaagtga ggcggaacag atgaacaaaa acgacgtatt gctaattgcg aaaaataaat
     2401 cactttattt ttttccttcc atccgctctt tagatgtttg gtccggaaaa gatttactac
     2461 agtcggaatt cattaaatca gctcgacaac agttggcaaa ttatcagctg atcgtttttt
     2521 tagattatgg atttgatatg gagatggctc gacgcgtgcg tccatttacc aatgcaaaaa
     2581 tcgtgttatt tttttggaac cactttaaag cagaacacga ggcgatgctg aagcaggcac
     2641 aagatgaacc ggcaatcgat gagatttatc attttgattt tttagaagcg aaagaacttg
     2701 gactaaaaca taatagttcc ttttattcga aacatatgga tttttcttct agcgaaccaa
     2761 agacggatct attttttggt gcaacaaata acggcagaaa agaacgggca gaaaaatata
     2821 agcaggaatt tacaaaaaga ggtgttacga ccaattactt tattcttccg cttcgaggaa
     2881 atgaacaacc tggatacctt acttatgaag aatatctgaa aaaaaccggg gaatcgaggg
     2941 ggattctaga actgttgcgg gaagggcagc aaggagtgac tttgcgaacg tttgaatcta
     3001 tgtattttca aaagaaacta gtgacagata atcaagccat agctcattat cagttttatc
     3061 attctgaaaa tatttttttg cttcaagaac gtgatttaga tgaattaccg gaatttttgc
     3121 atacgccatt tcaaccaatc ccatcagaac tgttggaatt ttttgatgca gaaaattggg
     3181 caaaacgttt tttcactact gactccgcta tttatgaaca atatgaatac gatctttcct
     3241 ctgttaaaaa taaataaaag tgccaaccaa tcatagatga aaataaacaa gcgcaattct
     3301 tatggcaaca tgagaattgt gtttttcttt ttgaactttc atgggggtaa aaaaatgaga
     3361 accagctgct gatccagctg attctcatca gagagccttt tattctgcgc caaggattcc
     3421 ttcttttgca tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt
     3481 ttgtcctttt cgccaaacgg caccattgat gtagtcgatt tcagtcaaac gatggttttt
     3541 gatcaggtct tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga
     3601 agcaatatgt tggtacactt cttcttgatc taatgtgatt ccttcttttg ctgcgataga
     3661 agcaaactca gatacaatcg ttcgaaccat gccctctgcc gctttttgcg cacctaattc
     3721 agcaatatta caatccaata tcgtgcataa tccatttaat gttccgttta cacaagcttt
     3781 tcgccaaatg gaataacgaa cattatgact atagattgga ttcaattttg cttcttgaaa
     3841 gacttctaca actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat
     3901 ttctccatca cctaataatg tgacttttcc gggaccagct agaccagctg tccacatcgt
     3961 gatcccaaat aagatatttt tctttggtac atatttttcc agtacatctt catgacctaa
     4021 tccattcagt aaacacaaaa cataagtatt ctcagtaata atggattgga tcgaacgaaa
     4081 catatcatct aattgattcg cttttgtcaa ggctacgatc aaatccacgt gtttgttgct
     4141 ttcgatgatt tcttctggtg aatagattgg cagttttgca actacttctt tgccattaaa
     4201 gtcagcaatc aatccatttg tacgaatggc ttcaatgtga gcaggccagc gatcgatcaa
     4261 gacgacctca tttccgcttt gatgcaacat taatccgatt cggctgccca ttgctccggc
     4321 tccagctatt gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta
     4381 tatagaaaaa cagaagaaaa tagcattgtt ttgtttgaaa aaagtgaaaa aaagtgtctt
     4441 acttaagaag aaagtgaggg atatagatgg atattttggc ggcaatcgat tcatttaaag
     4501 gaagtgcttc ttcatttgaa ttaaatcagg cagttctaaa tggttgggaa gaccgtaaag
     4561 gaagaaaaat caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt
     4621 acgcgctcgg gggaacctgg cgtacgattg atgggatgga tctcttattt cgtcagcgaa
     4681 aaatcagtta tttattgacg aactggcaag gaaaaaatac cgcagtcatt gaatctgcac
     4741 aagtattagg attagattta cttgataaac caacggatca aaccatccga cttgcttcta
     4801 gttatgcctt gggagagctt atcaaagata cactgcttca agaagtggaa caaatcattg
     4861 tcactttagg aggaagcgga tgtagcgatg gtggtctagg attactccaa tctttgggtg
     4921 cttctttaga agggtacaca gaaggaaatc ctttgttatc tactagaaaa cttcatttgg
     4981 atcgaaggaa cgttcttgtt tctaaagtaa aagtgatcat cgctgcagac gtgaccaacc
     5041 catatacagg tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaacggata
     5101 cgacgttagc tttcttggat aaacaagctg aaaagattgc tcagcagata aaagatgttt
     5161 gggaggtgga tttagatgac atcccaggaa gcggagcagc aggcggtatt ggcggagccc
     5221 tttttttgtt aggagcgaag atggtctctg gttttgagtt ggtcagccaa atcactggat
     5281 tagaaaaaca gatcgaacaa gcggatgtca tcattacagg agaagggcgg attgatcagc
     5341 aaacaatcca cggaaaagtt ccatatggcg tagcagtgct tgcaaaaaaa tatcgaaaac
     5401 caataatcgc tatttgcgga agtagagacc gagagctgga aggactcttt gattttctcc
     5461 ccattgtctt aagcattcaa ttaggaccaa tcgatttaga ggaagcaatg gaacacaggc
     5521 aaacattaga aaaaacagaa atcttaggac agacactcag cagatttttc aacattctcc
     5581 cgaagtagtt gacaaaaaca atttctttat gctatcgtaa tttcaaatat aggacaaagt
     5641 gagggatccg tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct
     5701 tgtggagaca agggagcagt gtgcagattt tcgggattgg cttggttctt tcgatgtatg
     5761 ggtctttttg tgtgccccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg
     5821 tcttttttta cgaggtgatg gaataatgtc taaaatcgaa ataaaaaatc tgacattcgg
     5881 ctatgacagc caaggcacat tattatttga acaagccaat ctaaactttg acacacaatg
     5941 gaaactagga cttatcggac gaaacggtcg aggaaagaca actttactga atatcctgca
     6001 aaacaaactc ccttatcaag ggcaagtgat ccatcagcaa gcgtttgtct atttcccgca
     6061 gcaaacaaaa gataaagagc gtttgaccta ttatgtgtta aatgagatta tggattttga
     6121 gatatgggaa attgaaagag agctccaact gatgcaaaca gatcctgaaa tcttatggag
     6181 agaattcagc acactatcag gaggagagaa gacaaaagtc ctactggcac tcttatttgt
     6241 ggatgacact catttcccgt taatcgatga accaacgaat catttggata tctctggcag
     6301 aaaacaagta gcggcttact tgaaaaagaa aaaacaagga ttcatcgtgg tgagtcatga
     6361 tcggggattt atcgatgaag tagtggacca tgttttagca atcgaaaaga gccaactgga
     6421 actttatcaa gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga
     6481 aatagctaaa aatgaaaaat tgaaaaaaga agtcagcagg ctaaagaaaa cagcagctga
     6541 aaaagcagaa tggtctcgtt cccgagaagg ggacaaaaca aagaaaaaag tcggattcat
     6601 cgatacggaa tctaggcgag tgaataaagg agcagtaggc gctgatgctg cacggacgat
     6661 gaaacgatct aaagcaatcg tgaatcggat ggaaacccag atcagtgaga aagaaaaatt
     6721 attaaaagat atcgagtata tcgatgcttt gacgatgaat agccaagtgt ctcatcataa
     6781 acggctgtta agcgtagaaa atcttcaatt agggtatgaa aatctgttat tcgagccagt
     6841 tcattttacg atcgaacctc atcagcggat ggcaatttca ggtcctaacg gcgcgggaaa
     6901 gtcatccatt atccattatc ttctggggac attcaacggc aaggttgtag gagaaaaatc
     6961 ccagcccaaa catttgacca ttagttacgt acgccaaaat tatgaagaca atcgagggac
     7021 attggcggaa ttcgcgaaga aaaaccgagt agactatcaa gcatttttga ataatctccg
     7081 aaagcttggg atggaaagag atgtttttca taataaaatc gaacagatga gtatgggaca
     7141 acggaaaaaa gtggaattgg ctaaatcttt atcccaacca gctgagctct atatatggga
     7201 tgaaccattg aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgattttgaa
     7261 cgtgaaacct gctatgttac tagtggaaca tgatcaaacc tttctagata aagtatctac
     7321 tgagattatt tctcttgaga aaatctaaac cttgtaatct caaagacaac aaggtatggt
     7381 taaggagaac tttgtcaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga
     7441 aacaacttgg aacgcacaag gattagtttg cgggcgagca gatgtgccgc tgactgaaaa
     7501 aggacagatg caggcacagg cactagcaga aaaggtagta gatttgcctg ttccaatcac
     7561 gaaaatcatc cattcccctc ttcaacgagc aagagataca gcacaagcag tagttgatcg
     7621 tctcaatttg ccgatgaccg ctgatgagcg attggtagaa atggattttg gggattatga
     7681 cggactgcca agcaaagatg aaaactttca aaaagcgcgt ttagcgtttg ctgtacgttt
     7741 cccaaatgga gagtcggtct tagatgtgta cgcacggatc gttcctttat tgaaagaatg
     7801 catggaagac gaagaaaatg tctacttgtt agtctgtcat aatgccttga taagagtgat
     7861 caatgcttat tttcatccaa tgcctaatga agggttcttt acattcatgg tagacaatac
     7921 agaattaatt tcttatgagt aattagactt ttgtgttatt taaataaaat gtttcaaggc
     7981 actgcttttg cttagcagtg ccttttttgt ggctgtgtca attcttttgg cattataaat
     8041 ccaattttta gaaataaaat ttaattattt taacaaaagt attgaaattt tgtttcttta
     8101 gatatatact ctagtcatag aaatgaagac ggatacagaa aaagagggat aatatgtttg
     8161 gcttttcaat atttatgaat gattctttgt cagaagagaa gaaacagtat atccgccaaa
     8221 tggcatccaa tggctttgtc ggtattttca cttccatgca tataccggaa gatgatgttt
     8281 cggcttatca aaatcgattg atcgaactag gaagcgaagc gaaaaagaat aaactagagt
     8341 tgatggtcga tatttcagga gaggcattga aacgagctgg cttttcatta gaagatctaa
     8401 cacccttgaa agcaattggt gtaacagggt taagaatgga ttatcatatt tctaatcagc
     8461 agatcgccga ctggtcacat caattgaaaa tcagtttgaa tgccagcacg atcacgccta
     8521 atgatatcga tgagttgaaa gaagcagcgg ctgatttctc acagattgaa gcttggcata
     8581 attattatcc acgtcttgaa acagggttgg acaaagaatg gtatcaggaa aaaaatcaat
     8641 ggctcaaaac tcaaggattc cttgtacaag gctttgtacc aggagataca gagttgagag
     8701 gtccactgta tcaaggatta ccaactttgg aagaacatcg agggatccat cctctggcag
     8761 gtgctcttga tctattagca agtaacacgg atattgttta tatcggagat gcaggtctgt
     8821 cagaacacgt tcaggagcaa tttgcttctt tccaaaaaga acagacagtc ttgctccata
     8881 cggaacctgt agatgaagtt ttttacgaat atatattagg aaaacatacg aatcggcaag
     8941 atgatgcaag agatgtcata cgaagcgcag atgcgcgttt tcgagagatt ccgccaatcc
     9001 cagtaagaaa t
//
LOCUS       DACECR010000254         4991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.guided.142, whole genome shotgun sequence.
ACCESSION   DACECR010000254 DACECR010000000
VERSION     DACECR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.guided.142"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..729)
                     /locus_tag="IWO17_002938"
     CDS             complement(<1..729)
                     /locus_tag="IWO17_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0291760.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKK"
     gene            1241..1876
                     /locus_tag="IWO17_002939"
     CDS             1241..1876
                     /locus_tag="IWO17_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0291761.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            2030..2107
                     /locus_tag="IWO17_002940"
                     /pseudo
     CDS             2030..2107
                     /locus_tag="IWO17_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            2352..2468
                     /locus_tag="IWO17_002941"
     CDS             2352..2468
                     /locus_tag="IWO17_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0291762.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            2484..4403
                     /gene="tet(M)"
                     /locus_tag="IWO17_002942"
     CDS             2484..4403
                     /gene="tet(M)"
                     /locus_tag="IWO17_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0291763.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            4522..4689
                     /locus_tag="IWO17_002943"
     CDS             4522..4689
                     /locus_tag="IWO17_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0291764.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(4749..>4991)
                     /locus_tag="IWO17_002944"
     CDS             complement(4749..>4991)
                     /locus_tag="IWO17_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0291765.1"
                     /translation="DPRYLTNIENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKS
                     TRRLQIEKYMDSFTDKELSLMESLASGINEARNIED"
BASE COUNT         1639 a          826 c          990 g         1536 t
ORIGIN      
        1 ctttttggtt gcctgttcct tttgtttttc atacaggcag aagtattttg tgcttgcctt
       61 tgaaccgata taaagggtgc ttgctaaatt acggtttttt ccgcttagtt ttccaccctg
      121 cacattttca taatttttac agcgacaatc cgcaccgcca tttctgtatt tttcagacaa
      181 aacgggaata tccaacagac cacacatatc attgattgcc aagtcaaaac gtcggataac
      241 accgtcacag tcaagacagc ggtttaaaaa tgaataccaa tctctgttct gtgcctgcaa
      301 gacatattcc atgttgcgtg agcctgcccc ttttaattcc aagaatacgc ccatgtgttc
      361 gtcatcagaa gccatgacct tgatttctcc gtaggcgtaa tgctctttat agccataata
      421 cccatagtca taatggataa aataatctgc tttcatgcca agcacctttc ggataatttc
      481 caatgcgtca gtcgttggat aacggacaga cagataatca acgagcagat aaaaaggctt
      541 atcgcagata taataattca gacaacgcag gataatattt tgtgtttcct tgctggcgtt
      601 ggcttttccg ttctcaaagc ggttgatact atatcttgat acatgggtaa gacttgcaag
      661 ttctccttgt gaaagaccgc ttgcaagacg tttgttcttc atttcaagtg caaattctaa
      721 attgttcatt gtattcctcc aatcccacaa tagccttgaa atacctgtaa acctctattc
      781 aaaaagtgtg atatgcacta tcttcttaaa acttctcatt actactcaca actttgggat
      841 tgtatggttt ttgacttgtc ttttgtgaaa tagtgccccc ctgttagata acgggggcac
      901 agacaaggct cgcaggctcg ccagccgaca aagcatttct tccccttgac acaaggtgtc
      961 atggcggtaa gcctgagagg cttccgccac cgtgtcagaa atactttgtc agcaagttct
     1021 gttttgcctt ttctaacaag ttccatttca accgatattc atatctacgg atttaatcag
     1081 ataggtattt ctttttgaat gtttttatat ttcattctat tgtgcttatt cgattacaag
     1141 aacatagtaa ttcttatatg cttatttgtc aatatctttt tttgccttta caaatcatat
     1201 taagcatgat agacttataa tagcgaagat ataacgagat atggagggtt atgcaatgaa
     1261 aataagccat ttaggaaata atatacagac cataagaaaa tttaggggaa tgaaacaaca
     1321 ggaacttgcg gacaaaatcg gtatcaatat gcagagcctt tccaagattg aaagagggtt
     1381 gaactatcct gcctatgaaa cgctggaaaa gataatggaa gtgctggacg ttacgcccaa
     1441 tgaattgttg tcgggagaat ggaagtatgt caatcaatct gaaaaggaag tctgtcagtt
     1501 tttaagagcc gaagaacgtc taaatgcaga actgaaacat ggacactatg ataacttctt
     1561 tgacagcgag gaagaatggc tggaatatga gttagaaaag ttacgggaat atattacaga
     1621 ctatatcaac gggaaaagca ttgaagcgtc cgacctttac ccaatcaaag aatttatcca
     1681 gcatttgaag tttcaaaaat tattagaccg ctatgatgat ttatacagta tggatatgtt
     1741 tggggaaagc atagagggac ataagtataa gacaccttat caagtcgtga aaatgataaa
     1801 cccgaactca aaagaagata tggaactgtt acgagaagta ttaaggaata acaattttga
     1861 tgatgaggac gagtaacttt tttcctcttg cgagaagata cagcaaacaa aaagcccgat
     1921 caagagtgca aagcaccacc aatggtggca aagctcttga cagggctttt tcttttctgt
     1981 tgtgggcaat caagaggaag aaagggataa aatctgattt gtggtaaatc aggcaaaggc
     2041 aacgcaggta tctcaatttg atttggtact tgaaaagaac gggagtaatt ggaagattat
     2101 agaataacaa atattggtac attattacag ctattttgta atcacgtact ctctttgata
     2161 aaaaattgga gattccttta caaatatgct cttacgtgct attatttaag tatctattta
     2221 aaaggagtta ataaatatgc ggcaaggtat tcttaaataa actgtcaatt tgatagtggg
     2281 aacaaataat tggatgtcct tttttaggag ggcttagttt tttgtaccca gtttaagaat
     2341 acctttatca tgtgattcta aagtatccgg agaatatctg tatgctttgt atgcctatgg
     2401 ttatgcataa aaatcccagt gataaaagta tttatcactg ggatttttat gcccttttgg
     2461 gtttttgaat ggaggaaaat cacatgaaaa ttattaatat tggagtttta gctcatgttg
     2521 atgcaggaaa aactacctta acagaaagct tattatataa cagtggagcg attacagaat
     2581 taggaagcgt ggacaaaggt acaacgagga cggataatac gcttttagaa cgtcagagag
     2641 gaattacaat tcagacagga ataacctctt ttcagtggga aaatacgaag gtgaacatca
     2701 tagacacgcc aggacatatg gatttcttag cagaagtata tcgttcatta tcagttttag
     2761 atggggcaat tctactgatt tctgcaaaag atggcgtaca agcacaaact cgtatattat
     2821 ttcatgcact taggaaaatg gggattccca caatcttttt tatcaataag attgaccaaa
     2881 atggaattga tttatcaacg gtttatcagg atattaaaga gaaactttct gccgaaattg
     2941 taatcaaaca gaaggtagaa ctgtatccta atatgtgtgt gacgaacttt accgaatctg
     3001 aacaatggga tacggtaata gagggaaacg atgacctttt agagaaatat atgtccggta
     3061 aatcattaga agcattggaa ctcgaacaag aggaaagcat aagatttcag aattgttctc
     3121 tgttccctct ttatcatgga agtgcaaaaa gtaatatagg gattgataac cttatagaag
     3181 ttattactaa taaattttat tcatcaacac atcgaggtcc gtctgaactt tgcggaaatg
     3241 ttttcaaaat tgaatataca aaaaaaagac aacgtcttgc atatatacgc ctttatagtg
     3301 gagtactaca tttacgagat tcggttagag tatcagaaaa agaaaaaata aaagttacag
     3361 aaatgtatac ttcaataaat ggtgaattat gtaagattga tagagcttat tctggagaaa
     3421 ttgttatttt gcaaaatgag tttttgaagt taaatagtgt tcttggagat acaaaactat
     3481 tgccacagag aaaaaagatt gaaaatccgc accctctact acaaacaact gttgaaccga
     3541 gtaaacctga acagagagaa atgttgcttg atgccctttt ggaaatctca gatagtgatc
     3601 cgcttctacg atattacgtg gattctacga cacatgaaat tatactttct ttcttaggga
     3661 aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac
     3721 taaaagagcc tacagtcatt tatatggaga gaccgttaaa aaatgcagaa tataccattc
     3781 acatcgaagt gccgccaaat cctttctggg cttccattgg tttatctgta tcaccgcttc
     3841 cgttgggaag tggaatgcag tatgagagct cggtttctct tggatactta aatcaatcgt
     3901 ttcaaaatgc agttatggag gggatacgct atggctgtga acaaggattg tatggttgga
     3961 atgtgacgga ctgtaaaatc tgttttaagt atggcttata ctatagccct gttagtaccc
     4021 cagcagattt tcggatgctt gctcctattg tattggaaca agtcttaaaa aaagctggaa
     4081 cagaattgtt agagccatat cttagtttta aaatttatgc gccacaggaa tatctttcac
     4141 gagcatacaa cgatgctcct aaatattgtg cgaacatcgt agacactcaa ttgaaaaata
     4201 atgaggtcat tcttagtgga gaaatccctg ctcggtgtat tcaagaatat cgtagtgatt
     4261 taactttctt tacaaatgga cgtagtgttt gtttaacaga gttaaaaggg taccatgtta
     4321 ctaccggtga acctgtttgc cagccccgtc gtccaaatag tcggatagat aaagtacgat
     4381 atatgttcaa taaaataact tagtgtattt tatgttgtta tataaatatg gtttcttgtt
     4441 aaataagatg aaatattttt taataaagat ttgaattaaa gtgtaaagga ggagatagtt
     4501 attataaact acaagtggat attgtgtcct gtatgtggaa ataaaacacg attaaagata
     4561 agggaagata ctgaattaaa aaaattcccc ctctattgtc cgaaatgcag acaagaaaat
     4621 ttaattgaaa taaagcagtt caaagtaact gtgattacag agccagacgc aaagacgcag
     4681 agccgataaa atgagattaa tacaatctca ttttatcggc tctttccgtt atgtatggat
     4741 tcttttaatt agtcttcgat gtttcttgct tcgttgatac cgctggctaa agattccatt
     4801 aaggatagtt ctttgtctgt aaagctatcc atgtatttct ctatctgtaa tcgtcgggtg
     4861 ctttttacca agttattagc aggtaagaaa aattcatcaa cggaaacatg aagtaacgat
     4921 acaaggtcat aaagaacttg tatgctgggg tgttgccctt tattttcaat attagttaag
     4981 taccgtgggt c
//
LOCUS       DACECR010000255         4991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.guided.143, whole genome shotgun sequence.
ACCESSION   DACECR010000255 DACECR010000000
VERSION     DACECR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.guided.143"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..729)
                     /locus_tag="IWO17_002945"
     CDS             complement(<1..729)
                     /locus_tag="IWO17_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0291766.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKK"
     gene            1241..1876
                     /locus_tag="IWO17_002946"
     CDS             1241..1876
                     /locus_tag="IWO17_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0291767.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            2030..2107
                     /locus_tag="IWO17_002947"
                     /pseudo
     CDS             2030..2107
                     /locus_tag="IWO17_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            2352..2468
                     /locus_tag="IWO17_002948"
     CDS             2352..2468
                     /locus_tag="IWO17_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0291768.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            2484..4403
                     /gene="tet(M)"
                     /locus_tag="IWO17_002949"
     CDS             2484..4403
                     /gene="tet(M)"
                     /locus_tag="IWO17_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0291769.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGHDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            4522..4689
                     /locus_tag="IWO17_002950"
     CDS             4522..4689
                     /locus_tag="IWO17_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0291770.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(4749..>4991)
                     /locus_tag="IWO17_002951"
     CDS             complement(4749..>4991)
                     /locus_tag="IWO17_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0291771.1"
                     /translation="DPRYLTNIENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKS
                     TRRLQIEKYMDSFTDKELSLMESLASGINEARNIED"
BASE COUNT         1638 a          827 c          990 g         1536 t
ORIGIN      
        1 ctttttggtt gcctgttcct tttgtttttc atacaggcag aagtattttg tgcttgcctt
       61 tgaaccgata taaagggtgc ttgctaaatt acggtttttt ccgcttagtt ttccaccctg
      121 cacattttca taatttttac agcgacaatc cgcaccgcca tttctgtatt tttcagacaa
      181 aacgggaata tccaacagac cacacatatc attgattgcc aagtcaaaac gtcggataac
      241 accgtcacag tcaagacagc ggtttaaaaa tgaataccaa tctctgttct gtgcctgcaa
      301 gacatattcc atgttgcgtg agcctgcccc ttttaattcc aagaatacgc ccatgtgttc
      361 gtcatcagaa gccatgacct tgatttctcc gtaggcgtaa tgctctttat agccataata
      421 cccatagtca taatggataa aataatctgc tttcatgcca agcacctttc ggataatttc
      481 caatgcgtca gtcgttggat aacggacaga cagataatca acgagcagat aaaaaggctt
      541 atcgcagata taataattca gacaacgcag gataatattt tgtgtttcct tgctggcgtt
      601 ggcttttccg ttctcaaagc ggttgatact atatcttgat acatgggtaa gacttgcaag
      661 ttctccttgt gaaagaccgc ttgcaagacg tttgttcttc atttcaagtg caaattctaa
      721 attgttcatt gtattcctcc aatcccacaa tagccttgaa atacctgtaa acctctattc
      781 aaaaagtgtg atatgcacta tcttcttaaa acttctcatt actactcaca actttgggat
      841 tgtatggttt ttgacttgtc ttttgtgaaa tagtgccccc ctgttagata acgggggcac
      901 agacaaggct cgcaggctcg ccagccgaca aagcatttct tccccttgac acaaggtgtc
      961 atggcggtaa gcctgagagg cttccgccac cgtgtcagaa atactttgtc agcaagttct
     1021 gttttgcctt ttctaacaag ttccatttca accgatattc atatctacgg atttaatcag
     1081 ataggtattt ctttttgaat gtttttatat ttcattctat tgtgcttatt cgattacaag
     1141 aacatagtaa ttcttatatg cttatttgtc aatatctttt tttgccttta caaatcatat
     1201 taagcatgat agacttataa tagcgaagat ataacgagat atggagggtt atgcaatgaa
     1261 aataagccat ttaggaaata atatacagac cataagaaaa tttaggggaa tgaaacaaca
     1321 ggaacttgcg gacaaaatcg gtatcaatat gcagagcctt tccaagattg aaagagggtt
     1381 gaactatcct gcctatgaaa cgctggaaaa gataatggaa gtgctggacg ttacgcccaa
     1441 tgaattgttg tcgggagaat ggaagtatgt caatcaatct gaaaaggaag tctgtcagtt
     1501 tttaagagcc gaagaacgtc taaatgcaga actgaaacat ggacactatg ataacttctt
     1561 tgacagcgag gaagaatggc tggaatatga gttagaaaag ttacgggaat atattacaga
     1621 ctatatcaac gggaaaagca ttgaagcgtc cgacctttac ccaatcaaag aatttatcca
     1681 gcatttgaag tttcaaaaat tattagaccg ctatgatgat ttatacagta tggatatgtt
     1741 tggggaaagc atagagggac ataagtataa gacaccttat caagtcgtga aaatgataaa
     1801 cccgaactca aaagaagata tggaactgtt acgagaagta ttaaggaata acaattttga
     1861 tgatgaggac gagtaacttt tttcctcttg cgagaagata cagcaaacaa aaagcccgat
     1921 caagagtgca aagcaccacc aatggtggca aagctcttga cagggctttt tcttttctgt
     1981 tgtgggcaat caagaggaag aaagggataa aatctgattt gtggtaaatc aggcaaaggc
     2041 aacgcaggta tctcaatttg atttggtact tgaaaagaac gggagtaatt ggaagattat
     2101 agaataacaa atattggtac attattacag ctattttgta atcacgtact ctctttgata
     2161 aaaaattgga gattccttta caaatatgct cttacgtgct attatttaag tatctattta
     2221 aaaggagtta ataaatatgc ggcaaggtat tcttaaataa actgtcaatt tgatagtggg
     2281 aacaaataat tggatgtcct tttttaggag ggcttagttt tttgtaccca gtttaagaat
     2341 acctttatca tgtgattcta aagtatccgg agaatatctg tatgctttgt atgcctatgg
     2401 ttatgcataa aaatcccagt gataaaagta tttatcactg ggatttttat gcccttttgg
     2461 gtttttgaat ggaggaaaat cacatgaaaa ttattaatat tggagtttta gctcatgttg
     2521 atgcaggaaa aactacctta acagaaagct tattatataa cagtggagcg attacagaat
     2581 taggaagcgt ggacaaaggt acaacgagga cggataatac gcttttagaa cgtcagagag
     2641 gaattacaat tcagacagga ataacctctt ttcagtggga aaatacgaag gtgaacatca
     2701 tagacacgcc aggacatatg gatttcttag cagaagtata tcgttcatta tcagttttag
     2761 atggggcaat tctactgatt tctgcaaaag atggcgtaca agcacaaact cgtatattat
     2821 ttcatgcact taggaaaatg gggattccca caatcttttt tatcaataag attgaccaaa
     2881 atggaattga tttatcaacg gtttatcagg atattaaaga gaaactttct gccgaaattg
     2941 taatcaaaca gaaggtagaa ctgtatccta atatgtgtgt gacgaacttt accgaatctg
     3001 aacaatggga tacggtaata gagggacacg atgacctttt agagaaatat atgtccggta
     3061 aatcattaga agcattggaa ctcgaacaag aggaaagcat aagatttcag aattgttctc
     3121 tgttccctct ttatcatgga agtgcaaaaa gtaatatagg gattgataac cttatagaag
     3181 ttattactaa taaattttat tcatcaacac atcgaggtcc gtctgaactt tgcggaaatg
     3241 ttttcaaaat tgaatataca aaaaaaagac aacgtcttgc atatatacgc ctttatagtg
     3301 gagtactaca tttacgagat tcggttagag tatcagaaaa agaaaaaata aaagttacag
     3361 aaatgtatac ttcaataaat ggtgaattat gtaagattga tagagcttat tctggagaaa
     3421 ttgttatttt gcaaaatgag tttttgaagt taaatagtgt tcttggagat acaaaactat
     3481 tgccacagag aaaaaagatt gaaaatccgc accctctact acaaacaact gttgaaccga
     3541 gtaaacctga acagagagaa atgttgcttg atgccctttt ggaaatctca gatagtgatc
     3601 cgcttctacg atattacgtg gattctacga cacatgaaat tatactttct ttcttaggga
     3661 aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac
     3721 taaaagagcc tacagtcatt tatatggaga gaccgttaaa aaatgcagaa tataccattc
     3781 acatcgaagt gccgccaaat cctttctggg cttccattgg tttatctgta tcaccgcttc
     3841 cgttgggaag tggaatgcag tatgagagct cggtttctct tggatactta aatcaatcgt
     3901 ttcaaaatgc agttatggag gggatacgct atggctgtga acaaggattg tatggttgga
     3961 atgtgacgga ctgtaaaatc tgttttaagt atggcttata ctatagccct gttagtaccc
     4021 cagcagattt tcggatgctt gctcctattg tattggaaca agtcttaaaa aaagctggaa
     4081 cagaattgtt agagccatat cttagtttta aaatttatgc gccacaggaa tatctttcac
     4141 gagcatacaa cgatgctcct aaatattgtg cgaacatcgt agacactcaa ttgaaaaata
     4201 atgaggtcat tcttagtgga gaaatccctg ctcggtgtat tcaagaatat cgtagtgatt
     4261 taactttctt tacaaatgga cgtagtgttt gtttaacaga gttaaaaggg taccatgtta
     4321 ctaccggtga acctgtttgc cagccccgtc gtccaaatag tcggatagat aaagtacgat
     4381 atatgttcaa taaaataact tagtgtattt tatgttgtta tataaatatg gtttcttgtt
     4441 aaataagatg aaatattttt taataaagat ttgaattaaa gtgtaaagga ggagatagtt
     4501 attataaact acaagtggat attgtgtcct gtatgtggaa ataaaacacg attaaagata
     4561 agggaagata ctgaattaaa aaaattcccc ctctattgtc cgaaatgcag acaagaaaat
     4621 ttaattgaaa taaagcagtt caaagtaact gtgattacag agccagacgc aaagacgcag
     4681 agccgataaa atgagattaa tacaatctca ttttatcggc tctttccgtt atgtatggat
     4741 tcttttaatt agtcttcgat gtttcttgct tcgttgatac cgctggctaa agattccatt
     4801 aaggatagtt ctttgtctgt aaagctatcc atgtatttct ctatctgtaa tcgtcgggtg
     4861 ctttttacca agttattagc aggtaagaaa aattcatcaa cggaaacatg aagtaacgat
     4921 acaaggtcat aaagaacttg tatgctgggg tgttgccctt tattttcaat attagttaag
     4981 taccgtgggt c
//
LOCUS       DACECR010000256         4163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.guided.157, whole genome shotgun sequence.
ACCESSION   DACECR010000256 DACECR010000000
VERSION     DACECR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.guided.157"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..527
                     /locus_tag="IWO17_002952"
     CDS             <1..527
                     /locus_tag="IWO17_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ0291772.1"
                     /translation="TNSYKDATAWLTGRYATAPNYGSTLNNVIETYNLTQYDTAPTST
                     SPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPNSGQPVYRLYNPYSGDH
                     HYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKTGTHHYTLSAKERDWLT
                     PMGWRYEGIGFYGY"
     gene            1014..1478
                     /locus_tag="IWO17_002953"
     CDS             1014..1478
                     /locus_tag="IWO17_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0291773.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            1462..2091
                     /locus_tag="IWO17_002954"
     CDS             1462..2091
                     /locus_tag="IWO17_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0291774.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            2445..3923
                     /gene="msr"
                     /locus_tag="IWO17_002955"
     CDS             2445..3923
                     /gene="msr"
                     /locus_tag="IWO17_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0291775.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3970..>4163
                     /locus_tag="IWO17_002956"
     CDS             3970..>4163
                     /locus_tag="IWO17_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0291776.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKK"
BASE COUNT         1413 a          695 c          852 g         1203 t
ORIGIN      
        1 atacgaactc ttacaaagat gctacggctt ggctaacagg tcgttatgcc actgcgccta
       61 attatggttc tacattgaac aatgtaatcg aaacctataa tctgacacag tatgatacag
      121 caccaacttc aacttctccg atgtatcgtc tgtataaccg tcatacagga gaacatttat
      181 atacattgaa tgccggagaa aaaaattatc tgccaactgt aggctgggaa tatgaaggga
      241 ttgcttggca agcaccaaac agcggacaac ctgtctaccg tttatacaat ccttacagtg
      301 gagaccatca ttacacaatg gcacaatctg agatcaactc tttaacaaaa atcggttggc
      361 gttatgaagg tctaagtttt tattctggtg gttccaagcc aatttatcga ctatttaatc
      421 caaatgaaaa aactggtacc catcactata ccttgagtgc taaggaaaga gattggctaa
      481 caccgatggg ctggagatat gaaggaatag gattttatgg ttattaaaaa gaggtagtga
      541 aaaagctgcc ctgcatcaag tagtaagaac agccaaacaa aaacagattt tcatgttttt
      601 tgtttggttg aacttattac cgcagatgca agtttttgaa caccgtttac tcaaaaaagg
      661 ttgtgacaac atcttgtcat aactttttta ttactgttta agcatactga tccaatttct
      721 aatattcgac gggattcttg caaaaggcga tttcggaaat ttttggcggt gtaaaattat
      781 ctactgcaga agagctatct tctatagttt gataagaatc aataatcttg gttttagcgt
      841 tgtgctttct ttatcatttt ttccgttcat tctacaaaaa atttcttttc tctatacttt
      901 tctattgaga gcgttttttt tattacacag ctgattcttt gacttttttc tttttttatt
      961 ctattattga caagtcaatc gttgacgagt caattattag aaaggaggaa attatgtctt
     1021 tagaagagat tagtcacctt ttatatcaaa tcaaattagc cgatcaaaaa atcgtccaga
     1081 tctttgaaga aaatatcggt ataagtctta ctagatacga gatcatgatg agactgaaag
     1141 aagaaaatcc attgctacaa agcagtttgc aagaaaagtt gcagattgat caaggagcag
     1201 ttactcgtca tctgaaggta ttggaagcaa atggctatgt ttcgcgtcag agaaatcctc
     1261 agaacaaccg ggaagtgtat gtagcgctaa cagaaaaagc ccagaagaaa atcaagcatt
     1321 gtgaaatgga ccataaagat acaggttccg tgctatcccc ggctttcagc gatgtagaaa
     1381 tgaaacaatt tctaacactt ttagataaat tgaataccca actttgcaat ataacaacag
     1441 aaacgaaaga ggttaataat aatggaacaa aaaaatgatt tttcaacaat tatgactggt
     1501 agaagatccg ttcgcgtata tgatgaaaca tacaaaatcc cacacgaaga aatgctggaa
     1561 atgatcaaag aagcgacgac tgcaccttct tctgtcaata tgaaaccatg gcgctttgtg
     1621 gtcgctgaaa gcgaggaagc aaaagaaatc ttgcgtcctt tgatccgttt caatacgaga
     1681 caaaatgata cttcttctgc aatggtcatg atttttggcg atatgcaatg ttacgaatat
     1741 ggcgaagaaa tctacaacca agcttacgaa tcagggaaga tgccaaaaga agtcagagat
     1801 caacaattag ctgcgatcat tccttattac aagagttttt caagagaaga aatgaacgat
     1861 gtagtcaaag tcgattctag cttagcggct atgcaattca tgttagttgc gcgtgatcat
     1921 ggctatgaaa caaatccaat tggtggattc gaagcagatc agttggcaga ggcatttggt
     1981 ttggataaag accgttatgt tccagttatt attttatcaa taggaaaagc ggtagaagaa
     2041 ggttacgagt ctgttcgttt agcaccagaa aagatcacta tgttcgaata aaaggttttc
     2101 agttactatt cagtagctat tgcttttttt gaatcgtgtg tggtaaccta ataaaaaagg
     2161 aggtcggaaa aatgactgca tcgatgaaat tacgtttcga acttttgaat aacaactaaa
     2221 taaattcaaa agacatgtat aaacaacaat ttcatggatg gagtatgtcc attttttcga
     2281 acgacagtag aataggtgaa agctatgaca gatttggtga tttagtcata gttatttcgt
     2341 tcagagtagg aaaagatgtg atgcagtttt tttgcaaaca gatttttctg ctctttttta
     2401 gtatcgataa ttgttgtttt acatgaaata ggagtgtaaa acatatggaa aatttagcag
     2461 taaatataac aaatctacaa gtcagctttg gcaaccagct agaactatct attgattctc
     2521 ttcgtgtcta tcagcaagat cgtataggga tcataggaga aaatggtgtc ggtaaatcga
     2581 ctttgctcaa actaatagcc ggtgaacttt ttcccgatca tgggaaaatc caaacagaga
     2641 tcaccttcaa ctacctgcct caattaacct atctcgctga agcaaaggac ctaaatttgg
     2701 aattagccag tcatttccag ttaagactgg aagaaacttc agagcggaaa tggagcggag
     2761 gggaagaacg aaagatcgag ttgatacgtc ttctttcttc ttatgaacaa gggatgcttc
     2821 tagacgagcc aacaacccat ctagatagaa aaagtattga tcgactgatt gaagagcttc
     2881 gttattatta tggtacgctg gtttttgtta gtcatgatcg ctattttcta gatgagctag
     2941 catcgaaaat ctgggaagta aaagacggag aaatccgaga gttttcgggg aattatagtg
     3001 cctatctcac tcaaaaggaa ttggagaaaa agactcagct acgagaagca gagtcgatca
     3061 tgaaagagaa aaaacgattg gaaaaatcga tccaagaaaa gaaaaaacaa gcggaaaagt
     3121 tggaaaaagt gtccagtaaa aagaaaaagc aacaaatcag actggatcgg ttgtcttcct
     3181 ctaaacaaaa agacagtgta caaaaagcca tccaaaagaa tgcgaaaaca ttagagagaa
     3241 gactccaaaa aataggagaa acaaccaagc cgcagcagat gaaacaaatc cgttttccag
     3301 taccaaaatc tcttgaactc cacagccgct atcccatcat gggacaaaat gtccaattgg
     3361 aaagaagtgg gagaacatta ctggtaaatg gtgattttca gttttcttta ggtaaaaaaa
     3421 tcgcgattgt cggtgaaaat ggttcaggta agacaacctt attagaacat atccgcaaac
     3481 aaggagaagg aatccttctc tctccgaaag taagctttca agtatatcag caaaagggtt
     3541 atcaaatgac atctgaagaa tccatcattc gttttgtcat gagacaaaca gagttttcag
     3601 aatcgcttgt ccgtagtttg ctgaatcact tagggtttgc tcaggaaact ttgacgaaac
     3661 cgttatgtac attaagtggg ggagaagcga cccgtctgac gattgctttg ctttttacta
     3721 agccaagtaa tgtgttgctg ttagatgaac cgactaattt tattgatatg gcaacgatcg
     3781 aagctttaga gaagctaatg caaatatatc cgggaacgat tttgtttact tctcatgatt
     3841 cttactttgt cgagcgtacg gctgatgaag tttatgaaat aaaagggcag aaaataaaaa
     3901 aagtacttac gagaaatttt taaatgtgct acgatgattt tcgtgacaaa tttaaaggag
     3961 tggtacagat tgcctacaaa taaaaaagaa ggaataattt ttacaacaat gatgtgtttt
     4021 ttgatggtct tggggatgag cgcgtataat ttatggctgc atcaagatct tcatttatca
     4081 agtttgttga ttggtctagt gcctggattc gtggtggctt tcattttgga tgtcttcatc
     4141 atcggtgtgc tggcgaaaaa aat
//
LOCUS       DACECR010000257         2598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.guided.178, whole genome shotgun sequence.
ACCESSION   DACECR010000257 DACECR010000000
VERSION     DACECR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.guided.178"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..677
                     /locus_tag="IWO17_002957"
     CDS             <1..677
                     /locus_tag="IWO17_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0291777.1"
                     /translation="NRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIKRVEQIIK
                     DTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIYPTALKKS
                     LMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAI
                     KLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESS
                     DERKQI"
     gene            658..1392
                     /locus_tag="IWO17_002958"
     CDS             658..1392
                     /locus_tag="IWO17_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0291778.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1425..2333
                     /locus_tag="IWO17_002959"
     CDS             1425..2333
                     /locus_tag="IWO17_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ0291779.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2330..2578
                     /locus_tag="IWO17_002960"
     CDS             2330..2578
                     /locus_tag="IWO17_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAQ0291780.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT          908 a          363 c          579 g          748 t
ORIGIN      
        1 agaatagaaa caaccttgtt gtacctcccg gagcgtgggg tgattgggtt aatggcggtg
       61 gatggttagt tataaacggg tgtcatgttg acttgatatt acgtgatata aaacgggtgg
      121 aacaaataat caaagatacg gagcaaggaa ttgttactgc caattatcag accgggcatc
      181 cccatggtta tataagtgct atgtatcgtg gagaattagc gattagtaaa atactatatg
      241 ctaagaacga aagcttatgc gaattaaaaa agcaggcaga aatttaccct actgctctaa
      301 agaaaagctt gatgaacttt tttatatttg aagctgaatt ctctttaatg tttgtaaaag
      361 caaatgcggg ggcagaggat aaatattata ttgcaggcca tgtttttcgt attatttcat
      421 gcttaaatca agtactattt gcatgtaata atgcttattg cattaacgaa aagaaagcta
      481 taaaactgct tgaaactttt gaatataaac ctgaaaaata tgccgaaaga gttaatcata
      541 tttttgaagt actcggtctt tctctttttg aatgctacga catgaccgag aaactttata
      601 aagaagtgaa tgaaattgta tcggagataa ataacttttt aaacgaggag agttcagatg
      661 aaagaaaaca aatatgatga taatatattt tttcaaaaat acagtcaaat gagtcgctcg
      721 cagaaaggac tggctggtgc gggagaatgg gagactttga aaaagatgct acctgatttt
      781 aagggtaagc gtgtgcttga tttaggatgc ggctatggat ggcactgtat atatgcgatg
      841 gaaaacggtg cttcctctgt agtaggtgtt gatatttctc ataaaatgct cgaagtagca
      901 aaaggaaaaa cccattttcc acagattgaa tatgaatgct gtgccataga agatgtggat
      961 ttcccagagg agagctttga tgtaatacta agttcgcttg cgtttcatta tgtagcagac
     1021 tatgagaatt taataaaaaa gatatatagg atgctgaagg ctggtggcaa tttagttttt
     1081 acagttgaac atcctgtttt tactgctcat ggaacacaag actggtatta taacgaaaaa
     1141 ggagaaatac tgcatttccc ggtggacaat tattattatg agggcaaacg gacagctatg
     1201 tttttggaag aaaaggttac aaaatatcat agaacactga ccacatatct aaatacactg
     1261 ctttcaaata gttttataat aaatcagatt gtggagccac agccgccaga gaacatgatg
     1321 gatattccgg ggatggcgga tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa
     1381 aagaagatgt aataatatag aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag
     1441 aaatgatgga tttagtactt tctttagcag aacaggatga acgtattcga attgtgaccc
     1501 ttgaggggtc acgcgcaaat attaatatac ctaaagatga atttcaggat tatgatatta
     1561 catattttgt aagtgatata gaaccgttta tatctaatga tgactggctt aatcaatttg
     1621 ggaatataat aatgatgcaa aagccggagg atatggaatt attcccacct gaagaaaagg
     1681 gattttccta tcttatgcta tttgatgatt acaataaaat tgatcttacc ttattgccct
     1741 tggaagagtt agataattac ctaaagggcg ataaattaat aaaggttcta attgataaag
     1801 attgtagaat taaaagggac atagttccga ctgatataga ttatcatgta agaaagccaa
     1861 gcgcaaggga gtatgatgat tgctgcaatg aattttggaa tgtaacacct tatgttatta
     1921 aaggattgtg ccgtaaggaa attttatttg ctattgatca ttttaatcag attgttcgcc
     1981 atgagctgct gagaatgata tcatggaagg tcggcatcga aacaggcttt aaattaagtg
     2041 taggcaagaa ctataagttt attgaaaggt atatatccga ggatttgtgg gagaaacttt
     2101 tgtccaccta ccggatggat tcctatgaaa acatatggga agcattattt ctatgccatc
     2161 aattgttcag ggcggtatcc ggtgaggtgg cggaaaggct tcattatgcc tatccggagt
     2221 atgataggaa tataacaaaa tataccaggg acatgtataa aaaatacact ggtaaaaccg
     2281 gctgcctgga tagcacatat gccgctgata tagaagagag gcgggaacag tgattacaga
     2341 aatgaaagca gggcacctga aagatatcga taaacccagc gaaccatttg aggtgatagg
     2401 taagattata ccgaggtatg aaaacgagaa ttggaccttt acagaattac tctatgaagc
     2461 gccatattta aaaagctacc aagacgaaga ggatgaagag gatgaggagg cagattgcct
     2521 tgaatatatt gacaatactg ataagataat atatctttta acccgtggtg ctagttaatt
     2581 ttaatatcct aaagaata
//
LOCUS       DACECR010000258         2358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.guided.185, whole genome shotgun sequence.
ACCESSION   DACECR010000258 DACECR010000000
VERSION     DACECR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.guided.185"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(89..1192)
                     /locus_tag="IWO17_002961"
     CDS             complement(89..1192)
                     /locus_tag="IWO17_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0291781.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            1745..2293
                     /gene="aac(6')"
                     /locus_tag="IWO17_002962"
     CDS             1745..2293
                     /gene="aac(6')"
                     /locus_tag="IWO17_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0291782.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          750 a          418 c          459 g          731 t
ORIGIN      
        1 agcttttttt gacaagaaaa accctcatca agcaaagaaa tgtcctcaat ttgatgacag
       61 cctgcttgat gggggtttta gcaattattt aactgatttt cttatatcct tgctggcttt
      121 cttgtgtagc atgataagtt tctacgtcaa gaatggcctg tttaaatttt tctcgggtga
      181 tttcataagg cacatgttta atatcatttg ttgccatggc tttatctaag attgcttcta
      241 aatcggtcgt gacattgaga ttcatgtcgg ttaacgatac tggaagttgg ttctttttca
      301 taaacacata gattttatca aacaaatcga actgctgatc catcatcaat aaggtcagta
      361 caccataact gacagatgct ccgtgaaggt atccctccat tttttctaga acagtcgtac
      421 cgtaatagaa tgagtgtgcc atagcagaat tatagttatt gttaactaaa acagaaacca
      481 atccggtagt aacaatgata tccgaaacga cttgttcaat tgcatatgag ggacgatcgt
      541 cggcactatc tttcaatgct tgcgtaccat aagtcaagag acgatcaatg cacatttttc
      601 cgatttggat tcctaatcta tccgtgtagc ctagatcatc gccacgagca gaaaaagtag
      661 attcgacttc tttggataaa gcatcgccaa tacctgccca taagtattta tcaggtgctt
      721 cggcaataat tgctgtatca atgaaggtgt gtacaggtgg ttttgaagga aagaagagtc
      781 cgtccattac gccttcttcg ttatagatga cacataccgc agtgacgggc gaacagttcg
      841 aaccaatagt gggaaaggtg aagactggtt tagctagttt ttcaccaatg acttttacag
      901 tatcgcaagc acggccacca ccgactgcaa acaggacgtc tgctttttgg acactttcat
      961 gctggatcaa ctgttcaata tttccataag ttgaattacc cccgtaccag agactggcga
     1021 caacctctat ttcctgtgtc aaagctactt ttattttgtt ttctgccttt gaaagtgcag
     1081 tcttccctcc aatcagcact acttttgtcc catattcttt gacgattcca tttgcttttt
     1141 catacgcatc tgttccaatg gtataatttg gtaagaaaac actttgactc atattcattt
     1201 ccttctttct tcgttgaaac gggttaaaat tttaaagagt gatcgatctg actgcggctg
     1261 tagatggagt agaggtgaac ctaagacagg ataagtgatg aataaggtgg catgaacacc
     1321 ttgaaatcat caataagtat cctaaaaacg aacaagtaca aaaacattga aaacgacaat
     1381 aatgaatttt tgttcctctt agtatttcta ccgattccct ttgctttttt gtaattatct
     1441 aaaaattcta aaaattaaga ttattatatg aaaacaggtt ttatttgtca attcataatt
     1501 ggtttttttt aaagcaaaag ataatctata tcatgatgaa caaacagagt tttttcctcc
     1561 aattgatggc tttcctgatc tttcctattt tgaataaagt gtttctgtat aattttttta
     1621 ttttataagt aagataaata atgaaagaat agagatactg attggtagct aaagattttt
     1681 gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac aagggggaag
     1741 acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc agctttctga
     1801 tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag aagtagaaga
     1861 aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt tagtaggatt
     1921 tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat tagttgtaga
     1981 aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag aaaaagaagt
     2041 agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc atggaacaac
     2101 gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca tccagaacct
     2161 tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag gtgtcttacc
     2221 aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta ttcctcgacc
     2281 aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga aataagaagg
     2341 aatttacgaa aattactt
//
LOCUS       DACECR010000259         2286 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.guided.189, whole genome shotgun sequence.
ACCESSION   DACECR010000259 DACECR010000000
VERSION     DACECR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.guided.189"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..537
                     /locus_tag="IWO17_002963"
     CDS             <1..537
                     /locus_tag="IWO17_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0291783.1"
                     /translation="RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAV
                     ITVSHDRYFLDKVADKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKE
                     PAEKKKLSYMEQKEWETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEA
                     QLEEKMARWEYLSEWVED"
     gene            549..1496
                     /locus_tag="IWO17_002964"
     CDS             549..1496
                     /locus_tag="IWO17_002964"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0291784.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            1516..2043
                     /locus_tag="IWO17_002965"
     CDS             1516..2043
                     /locus_tag="IWO17_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0291785.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(2106..>2286)
                     /locus_tag="IWO17_002966"
     CDS             complement(2106..>2286)
                     /locus_tag="IWO17_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0291786.1"
                     /translation="PLYHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFV
                     MLGAGFMYFSVLFFT"
BASE COUNT          789 a          397 c          501 g          599 t
ORIGIN      
        1 cgccggttat ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa
       61 ccgaccaatg atttggacat agcaacattg acgattttgg aagattattt tagaagtttt
      121 ccaggtgctg tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag
      181 ttattagtat tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat
      241 ttactgaagc aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc
      301 aaggaacctg ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt
      361 gaagatgaga ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat
      421 caaggagata atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca
      481 caacttgaag aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag
      541 gaaaaaggat ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc
      601 gtaaagaaga cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg
      661 atctgcaaaa agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa
      721 gcgaattgct ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa
      781 accatatttg ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac
      841 cagatatgac tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata
      901 aaaagcagag caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat
      961 acggcgatct tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg
     1021 gctttatcga tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa
     1081 gattgatcgt ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc
     1141 atacgatgtt tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca
     1201 gtggggatgt gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc
     1261 taattgcaca tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc
     1321 atctttatat caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat
     1381 ttccgacttt agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata
     1441 tccaattaga aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc
     1501 agaaaggatg agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga
     1561 aaagaagacc gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca
     1621 gaagcaaata cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg
     1681 ccgaatcgtc agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat
     1741 gtcatgcata gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc
     1801 atcgctggag ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt
     1861 acagtcatcc atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat
     1921 tggtcactga tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa
     1981 tttgaaacgt atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata
     2041 taaaaaagct atggatagac tagctgttat ttagagctgt cccatccata gctttatttt
     2101 ttttattaag taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac
     2161 aaatgccaaa caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc
     2221 gtgtaagaaa gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa
     2281 agagga
//
LOCUS       DACECR010000260         1521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.guided.207, whole genome shotgun sequence.
ACCESSION   DACECR010000260 DACECR010000000
VERSION     DACECR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.guided.207"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            266..1141
                     /locus_tag="IWO17_002967"
                     /note="aph(2'')-Ia"
     CDS             266..1141
                     /locus_tag="IWO17_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ0291787.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
     gene            complement(1271..>1521)
                     /locus_tag="IWO17_002968"
     CDS             complement(1271..>1521)
                     /locus_tag="IWO17_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0291788.1"
                     /translation="LFDSRFKTLQQNPFQYTVQGNSHNRLKSTNLIERLNQEVRRREK
                     IIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          610 a          139 c          257 g          515 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccgt acaagatata ttaaattgat
       61 ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa
      121 taatccaaga gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc
      181 agaacatgaa ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga
      241 tgataatgcc acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt
      301 caaagtagat agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa
      361 taatgaatac atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga
      421 aaaagcaata tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat
      481 tgaatattcg tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac
      541 ttttttaaca ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg
      601 agatattgcc agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg
      661 tactattgat aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta
      721 taatgattta actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc
      781 aacaacagtt tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct
      841 attgttagat ggcaataata gattaactgg aataattgat tttggagatt ctggaattat
      901 agatgaatat tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa
      961 ttttggagaa gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata
     1021 tcaagatata gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat
     1081 taaacaggaa tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg
     1141 attatataat atatgaaaag ctattataaa agacattagt attaaatagt ttaaaaaaat
     1201 gaaaaataat aaaggaagtg agtcaattcc agactcctgt gtaaaatgct atacaatgtt
     1261 tttaccattt ctacttatca aaattgatgt attttcttga agaataaatc cattcatcat
     1321 gtaggtccat aagaacggct ccaattaagc gattggctga tgtttgattg gggaagatgc
     1381 gaataatctt ttctcttctg cgtacttctt gattcagtcg ttcaattaga ttggtactct
     1441 ttagtcgatt gtgggaattt ccttgtacgg tatattgaaa ggggttctgt tgcaaagttt
     1501 taaatctact atcaaataag g
//
LOCUS       DACECR010000261         1504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.guided.208, whole genome shotgun sequence.
ACCESSION   DACECR010000261 DACECR010000000
VERSION     DACECR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.guided.208"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IWO17_002969"
                     /pseudo
     CDS             262..373
                     /locus_tag="IWO17_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IWO17_002970"
     CDS             394..477
                     /locus_tag="IWO17_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAQ0291789.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IWO17_002971"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IWO17_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0291790.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DACECR010000262         1348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E42804_2017
            SAMN12639868-rid9879953.guided.217, whole genome shotgun sequence.
ACCESSION   DACECR010000262 DACECR010000000
VERSION     DACECR010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639868
            Sequence Read Archive: SRR10023233
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741758.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741758.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:27:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,951
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 29 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 18 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639868-rid9879953.guided.217"
                     /isolate="E42804_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            45..296
                     /locus_tag="IWO17_002972"
                     /pseudo
     CDS             45..296
                     /locus_tag="IWO17_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            389..1183
                     /locus_tag="IWO17_002973"
                     /note="aph(3')-IIIa"
     CDS             389..1183
                     /locus_tag="IWO17_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0291791.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          233 c          322 g          369 t
ORIGIN      
        1 attttcctct aggaaaaaat taactagcac cacgggttat cttttatata gaagatatcg
       61 ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc gcttatcaat atatctatag
      121 aatgggcaaa gcataaaaac ttgcatggac taatgcttga aacccaggac aataacctta
      181 tagcttgtaa attctatcat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat
      241 acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg
      301 caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat
      361 actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa
      421 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
      481 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
      541 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
      601 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
      661 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
      721 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
      781 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
      841 ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct
      901 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
      961 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
     1021 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
     1081 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
     1141 ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag atttagatgt
     1201 ctaaaaagct ttaactacaa gctttttaga catctaatct tttctgaagt acatccgcaa
     1261 ctgtccatac tctgatgttt tatatctttt ctaaaagttc gctagatagg ggtcccgagc
     1321 gcctacgagg aatttgtatc gataagaa
//
